LOCUS       DAZPEP010000001        80192 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZPEP010000001 DAZPEP010000000
VERSION     DAZPEP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.001"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            102..386
                     /gene="pduA"
                     /locus_tag="ACHUT9_000001"
     CDS             102..386
                     /gene="pduA"
                     /locus_tag="ACHUT9_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HFR1346588.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            383..1195
                     /gene="pduB"
                     /locus_tag="ACHUT9_000002"
                     /pseudo
     CDS             383..1195
                     /gene="pduB"
                     /locus_tag="ACHUT9_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
     gene            1214..2878
                     /gene="pduC"
                     /locus_tag="ACHUT9_000003"
     CDS             1214..2878
                     /gene="pduC"
                     /locus_tag="ACHUT9_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HFR1346589.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTDGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGGFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2888..3556
                     /locus_tag="ACHUT9_000004"
     CDS             2888..3556
                     /locus_tag="ACHUT9_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HFR1346590.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3571..4089
                     /gene="pduE"
                     /locus_tag="ACHUT9_000005"
     CDS             3571..4089
                     /gene="pduE"
                     /locus_tag="ACHUT9_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HFR1346591.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4099..5931
                     /locus_tag="ACHUT9_000006"
     CDS             4099..5931
                     /locus_tag="ACHUT9_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HFR1346592.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5921..6271
                     /locus_tag="ACHUT9_000007"
     CDS             5921..6271
                     /locus_tag="ACHUT9_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HFR1346593.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6291..6566
                     /gene="pduJ"
                     /locus_tag="ACHUT9_000008"
     CDS             6291..6566
                     /gene="pduJ"
                     /locus_tag="ACHUT9_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HFR1346594.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6590..7012
                     /locus_tag="ACHUT9_000009"
     CDS             6590..7012
                     /locus_tag="ACHUT9_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFR1346595.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            7015..7647
                     /locus_tag="ACHUT9_000010"
     CDS             7015..7647
                     /locus_tag="ACHUT9_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HFR1346596.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7644..8135
                     /gene="pduM"
                     /locus_tag="ACHUT9_000011"
     CDS             7644..8135
                     /gene="pduM"
                     /locus_tag="ACHUT9_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HFR1346597.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8139..8414
                     /gene="pduN"
                     /locus_tag="ACHUT9_000012"
     CDS             8139..8414
                     /gene="pduN"
                     /locus_tag="ACHUT9_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HFR1346598.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8426..9433
                     /gene="pduO"
                     /locus_tag="ACHUT9_000013"
     CDS             8426..9433
                     /gene="pduO"
                     /locus_tag="ACHUT9_000013"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HFR1346599.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9430..10815
                     /gene="pduP"
                     /locus_tag="ACHUT9_000014"
                     /pseudo
     CDS             9430..10815
                     /gene="pduP"
                     /locus_tag="ACHUT9_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10826..11938
                     /locus_tag="ACHUT9_000015"
     CDS             10826..11938
                     /locus_tag="ACHUT9_000015"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0051144 - propanediol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-propanol dehydrogenase PduQ"
                     /protein_id="HFR1346600.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLEELL"
     gene            11935..13278
                     /locus_tag="ACHUT9_000016"
     CDS             11935..13278
                     /locus_tag="ACHUT9_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1346601.1"
                     /translation="MNVGLISCDDQTIRERVQAAGVVGAGGAGFPTHIKLQARADTFL
                     VNAAECEPMLKVDQQLMAQQASRLLRGVHYAMKATGASSGIIALKEKYQRAINALTPL
                     LPPDIRLHILPDVYPAGDEVLTIWMATGRRVPPAALPVSVGVVVNNVQTVLNIARAVE
                     QQYPVTRRTLTVNGAVAKPITVTVPIGMSLREVLALAGGATVDAPGFINGGPMMGSLI
                     TSLDTPVSKTTGGLLVLPNSHSLIQRRMQNDRSVLAVAKTVCEQCRLCTDLCPRHLIG
                     HELAPHLLVRAVNYQQLATPQLLLTALTCSECNVCASVACPVGISPMRINRLLKQELR
                     AQNLRYEGALNPADPMANYRLIPVKRLVTRLGLTPWYQDAPLSEQVPQPEKVTLLLRQ
                     HIGASAIACVQKGDRVVHGQCVGQIPHGTLGAPIHASIDGMVSDVTENAITLVRG"
     gene            13281..13835
                     /gene="pduT"
                     /locus_tag="ACHUT9_000017"
     CDS             13281..13835
                     /gene="pduT"
                     /locus_tag="ACHUT9_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HFR1346602.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13835..14185
                     /gene="pduU"
                     /locus_tag="ACHUT9_000018"
     CDS             13835..14185
                     /gene="pduU"
                     /locus_tag="ACHUT9_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HFR1346603.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14190..14633
                     /locus_tag="ACHUT9_000019"
     CDS             14190..14633
                     /locus_tag="ACHUT9_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFR1346604.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14734..15063)
                     /locus_tag="ACHUT9_000020"
     CDS             complement(14734..15063)
                     /locus_tag="ACHUT9_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFR1346605.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15235..16293)
                     /locus_tag="ACHUT9_000021"
     CDS             complement(15235..16293)
                     /locus_tag="ACHUT9_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1346606.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16491..16964)
                     /gene="sbmC"
                     /locus_tag="ACHUT9_000022"
     CDS             complement(16491..16964)
                     /gene="sbmC"
                     /locus_tag="ACHUT9_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFR1346607.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17083..18249)
                     /gene="dacD"
                     /locus_tag="ACHUT9_000023"
     CDS             complement(17083..18249)
                     /gene="dacD"
                     /locus_tag="ACHUT9_000023"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFR1346608.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18458..19885
                     /gene="sbcB"
                     /locus_tag="ACHUT9_000024"
     CDS             18458..19885
                     /gene="sbcB"
                     /locus_tag="ACHUT9_000024"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFR1346609.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19928..20155)
                     /gene="tsuB"
                     /locus_tag="ACHUT9_000025"
     CDS             complement(19928..20155)
                     /gene="tsuB"
                     /locus_tag="ACHUT9_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFR1346610.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20169..21227)
                     /gene="tsuA"
                     /locus_tag="ACHUT9_000026"
     CDS             complement(20169..21227)
                     /gene="tsuA"
                     /locus_tag="ACHUT9_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFR1346611.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21406..22764)
                     /gene="plaP"
                     /locus_tag="ACHUT9_000027"
     CDS             complement(21406..22764)
                     /gene="plaP"
                     /locus_tag="ACHUT9_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFR1346612.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22754..22816)
                     /gene="yoeI"
                     /locus_tag="ACHUT9_000028"
     CDS             complement(22754..22816)
                     /gene="yoeI"
                     /locus_tag="ACHUT9_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFR1346613.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(23031..23960)
                     /locus_tag="ACHUT9_000029"
     CDS             complement(23031..23960)
                     /locus_tag="ACHUT9_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1346614.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(24006..24830)
                     /locus_tag="ACHUT9_000030"
     CDS             complement(24006..24830)
                     /locus_tag="ACHUT9_000030"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1346615.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(24913..25167)
                     /gene="yoeB"
                     /locus_tag="ACHUT9_000031"
     CDS             complement(24913..25167)
                     /gene="yoeB"
                     /locus_tag="ACHUT9_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFR1346616.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(25164..25415)
                     /gene="yefM"
                     /locus_tag="ACHUT9_000032"
     CDS             complement(25164..25415)
                     /gene="yefM"
                     /locus_tag="ACHUT9_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFR1346617.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            25698..25748
                     /gene="hisL"
                     /locus_tag="ACHUT9_000033"
     CDS             25698..25748
                     /gene="hisL"
                     /locus_tag="ACHUT9_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFR1346618.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    25724..25847
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            25894..26793
                     /gene="hisG"
                     /locus_tag="ACHUT9_000034"
     CDS             25894..26793
                     /gene="hisG"
                     /locus_tag="ACHUT9_000034"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFR1346619.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            26799..28103
                     /gene="hisD"
                     /locus_tag="ACHUT9_000035"
     CDS             26799..28103
                     /gene="hisD"
                     /locus_tag="ACHUT9_000035"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFR1346620.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            28100..29170
                     /gene="hisC"
                     /locus_tag="ACHUT9_000036"
     CDS             28100..29170
                     /gene="hisC"
                     /locus_tag="ACHUT9_000036"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFR1346621.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            29170..30237
                     /gene="hisB"
                     /locus_tag="ACHUT9_000037"
     CDS             29170..30237
                     /gene="hisB"
                     /locus_tag="ACHUT9_000037"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFR1346622.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            30237..30827
                     /gene="hisH"
                     /locus_tag="ACHUT9_000038"
     CDS             30237..30827
                     /gene="hisH"
                     /locus_tag="ACHUT9_000038"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFR1346623.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            30827..31564
                     /gene="hisA"
                     /locus_tag="ACHUT9_000039"
     CDS             30827..31564
                     /gene="hisA"
                     /locus_tag="ACHUT9_000039"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFR1346624.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            31546..32322
                     /gene="hisF"
                     /locus_tag="ACHUT9_000040"
     CDS             31546..32322
                     /gene="hisF"
                     /locus_tag="ACHUT9_000040"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFR1346625.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            32316..32927
                     /gene="hisIE"
                     /locus_tag="ACHUT9_000041"
     CDS             32316..32927
                     /gene="hisIE"
                     /locus_tag="ACHUT9_000041"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFR1346626.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(33024..34001)
                     /gene="wzzB"
                     /locus_tag="ACHUT9_000042"
     CDS             complement(33024..34001)
                     /gene="wzzB"
                     /locus_tag="ACHUT9_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFR1346627.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(34147..35313)
                     /gene="ugd"
                     /locus_tag="ACHUT9_000043"
     CDS             complement(34147..35313)
                     /gene="ugd"
                     /locus_tag="ACHUT9_000043"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFR1346628.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(35563..36969)
                     /gene="gndA"
                     /locus_tag="ACHUT9_000044"
     CDS             complement(35563..36969)
                     /gene="gndA"
                     /locus_tag="ACHUT9_000044"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFR1346629.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(37133..38503)
                     /gene="cpsG"
                     /locus_tag="ACHUT9_000045"
                     /gene_synonym="manB"
     CDS             complement(37133..38503)
                     /gene="cpsG"
                     /locus_tag="ACHUT9_000045"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFR1346630.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(38608..40044)
                     /gene="cpsB"
                     /locus_tag="ACHUT9_000046"
     CDS             complement(38608..40044)
                     /gene="cpsB"
                     /locus_tag="ACHUT9_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFR1346631.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(40047..41270)
                     /gene="wcaI"
                     /locus_tag="ACHUT9_000047"
     CDS             complement(40047..41270)
                     /gene="wcaI"
                     /locus_tag="ACHUT9_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFR1346632.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(41267..41746)
                     /gene="gmm"
                     /locus_tag="ACHUT9_000048"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(41267..41746)
                     /gene="gmm"
                     /locus_tag="ACHUT9_000048"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFR1346633.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(41749..42714)
                     /gene="fcl"
                     /locus_tag="ACHUT9_000049"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(41749..42714)
                     /gene="fcl"
                     /locus_tag="ACHUT9_000049"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFR1346634.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(42717..43838)
                     /gene="gmd"
                     /locus_tag="ACHUT9_000050"
     CDS             complement(42717..43838)
                     /gene="gmd"
                     /locus_tag="ACHUT9_000050"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFR1346635.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(43864..44412)
                     /gene="wcaF"
                     /locus_tag="ACHUT9_000051"
     CDS             complement(43864..44412)
                     /gene="wcaF"
                     /locus_tag="ACHUT9_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFR1346636.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(44428..45174)
                     /gene="wcaE"
                     /locus_tag="ACHUT9_000052"
     CDS             complement(44428..45174)
                     /gene="wcaE"
                     /locus_tag="ACHUT9_000052"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFR1346637.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(45185..46402)
                     /gene="wcaD"
                     /locus_tag="ACHUT9_000053"
     CDS             complement(45185..46402)
                     /gene="wcaD"
                     /locus_tag="ACHUT9_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFR1346638.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(46377..47594)
                     /gene="wcaC"
                     /locus_tag="ACHUT9_000054"
     CDS             complement(46377..47594)
                     /gene="wcaC"
                     /locus_tag="ACHUT9_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFR1346639.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(47591..48079)
                     /gene="wcaB"
                     /locus_tag="ACHUT9_000055"
     CDS             complement(47591..48079)
                     /gene="wcaB"
                     /locus_tag="ACHUT9_000055"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFR1346640.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(48082..48921)
                     /gene="wcaA"
                     /locus_tag="ACHUT9_000056"
     CDS             complement(48082..48921)
                     /gene="wcaA"
                     /locus_tag="ACHUT9_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFR1346641.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(49014..51176)
                     /gene="wzc"
                     /locus_tag="ACHUT9_000057"
     CDS             complement(49014..51176)
                     /gene="wzc"
                     /locus_tag="ACHUT9_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFR1346642.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(51179..51622)
                     /gene="wzb"
                     /locus_tag="ACHUT9_000058"
     CDS             complement(51179..51622)
                     /gene="wzb"
                     /locus_tag="ACHUT9_000058"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFR1346643.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(51628..52767)
                     /gene="wza"
                     /locus_tag="ACHUT9_000059"
     CDS             complement(51628..52767)
                     /gene="wza"
                     /locus_tag="ACHUT9_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFR1346644.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            53076..53225
                     /locus_tag="ACHUT9_000060"
     CDS             53076..53225
                     /locus_tag="ACHUT9_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1346645.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            53426..55009
                     /locus_tag="ACHUT9_000061"
     CDS             53426..55009
                     /locus_tag="ACHUT9_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1346646.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(55283..57136)
                     /gene="asmA"
                     /locus_tag="ACHUT9_000062"
     CDS             complement(55283..57136)
                     /gene="asmA"
                     /locus_tag="ACHUT9_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFR1346647.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(57158..57739)
                     /gene="dcd"
                     /locus_tag="ACHUT9_000063"
     CDS             complement(57158..57739)
                     /gene="dcd"
                     /locus_tag="ACHUT9_000063"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFR1346648.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(57831..58472)
                     /gene="udk"
                     /locus_tag="ACHUT9_000064"
     CDS             complement(57831..58472)
                     /gene="udk"
                     /locus_tag="ACHUT9_000064"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFR1346649.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            58790..62107
                     /locus_tag="ACHUT9_000065"
     CDS             58790..62107
                     /locus_tag="ACHUT9_000065"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1346650.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(62217..63065)
                     /gene="alkA"
                     /locus_tag="ACHUT9_000066"
     CDS             complement(62217..63065)
                     /gene="alkA"
                     /locus_tag="ACHUT9_000066"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFR1346651.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            63199..64551
                     /gene="yegD"
                     /locus_tag="ACHUT9_000067"
     CDS             63199..64551
                     /gene="yegD"
                     /locus_tag="ACHUT9_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1346652.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(64564..66504)
                     /gene="yegI"
                     /locus_tag="ACHUT9_000068"
     CDS             complement(64564..66504)
                     /gene="yegI"
                     /locus_tag="ACHUT9_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFR1346653.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(66501..67262)
                     /gene="pphC"
                     /locus_tag="ACHUT9_000069"
     CDS             complement(66501..67262)
                     /gene="pphC"
                     /locus_tag="ACHUT9_000069"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFR1346654.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(67259..67918)
                     /locus_tag="ACHUT9_000070"
     CDS             complement(67259..67918)
                     /locus_tag="ACHUT9_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1346655.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(68139..68198)
                     /gene="ibsA"
                     /locus_tag="ACHUT9_000071"
     CDS             complement(68139..68198)
                     /gene="ibsA"
                     /locus_tag="ACHUT9_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFR1346656.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(68471..68527)
                     /locus_tag="ACHUT9_000072"
     CDS             complement(68471..68527)
                     /locus_tag="ACHUT9_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1346657.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(68798..68854)
                     /locus_tag="ACHUT9_000073"
     CDS             complement(68798..68854)
                     /locus_tag="ACHUT9_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1346658.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            69133..70374
                     /gene="mdtA"
                     /locus_tag="ACHUT9_000074"
     CDS             69133..70374
                     /gene="mdtA"
                     /locus_tag="ACHUT9_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFR1346659.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            70374..73496
                     /gene="mdtB"
                     /locus_tag="ACHUT9_000075"
     CDS             70374..73496
                     /gene="mdtB"
                     /locus_tag="ACHUT9_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFR1346660.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            73497..76574
                     /gene="mdtC"
                     /locus_tag="ACHUT9_000076"
     CDS             73497..76574
                     /gene="mdtC"
                     /locus_tag="ACHUT9_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFR1346661.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            76575..77990
                     /locus_tag="ACHUT9_000077"
     CDS             76575..77990
                     /locus_tag="ACHUT9_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1346662.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            77987..79390
                     /gene="baeS"
                     /locus_tag="ACHUT9_000078"
     CDS             77987..79390
                     /gene="baeS"
                     /locus_tag="ACHUT9_000078"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFR1346663.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            79387..80109
                     /gene="baeR"
                     /locus_tag="ACHUT9_000079"
     CDS             79387..80109
                     /gene="baeR"
                     /locus_tag="ACHUT9_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFR1346664.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        19327 a        21229 c        20486 g        19150 t
ORIGIN      
        1 tgttctctat taatttctct cattagccga ttaagtcagc cattcttttt cctcgctttt
       61 ttttatagtc atatcgtcaa aaaaatccag gcgaggtctt tatgcaacaa gaagcgttag
      121 gaatggtaga aaccaaaggc ttaactgccg ccatagaggc cgcagacgcc atggtgaagt
      181 cggcaaatgt tttgctggtc ggctacgaaa aaataggttc cgggctggtg accgtcatcg
      241 ttcgtggtga tgtcggggcg gtaaaagcgg caacagacgc gggtgccgca gctgcgcata
      301 acgtcggcga agtaaaagcc gtacacgtta ttccccgccc acataccgat gtcgaaaaaa
      361 tcttaccaaa gggaattaac ccatgagcag caatgaactg gtcgaacaga tcatggcgca
      421 ggtcatcgct cgcgtagcga cgccagagca tgaggttctc ccgaaaacca ctcatcaaat
      481 acgatagacg gctatggcag aaaaaagctg cacgctaaca gaatttgttg gcaccgctat
      541 tggcgacact gtaggtctgg tcattgcgaa cgttgatagc gccctgttgg acgcgatgaa
      601 acttgaaaaa cgctatcgtt ccattggcat tctgggtgcg cgtattggcg cagggccaca
      661 cattatggcc gctgatgaag cggtaaaagc caccaacact gaagttatta gcattgaact
      721 tcctcgcgat actaaaggcg gtgccggaca cggctcactg atcctcttag gtggcaacga
      781 tgtttccgac gtgaaacgcg gtattgaagt cgccctgaaa gaactcgatc ggacctttgg
      841 cgatgtctat gccaacgaag ccggacatct tgaactgcaa tataccgccc gcgccagcta
      901 tgcactggaa aaagcgtttg gtgcaccact gggacgggct tgcggcgtta ttgtcggcgc
      961 tccggctgct gtgggtgttc tgatggccga caccgcgctg aagtccgcca atgtggatgt
     1021 tgtggcatac agctccccag ctcaaggcac cagctacagc aatgaagtca ttctgattat
     1081 ctcgggtgac tccggcgcag tccgccaggc ggtcatctcc gcacgcgaaa tcggtaaaac
     1141 cgtgctttcg actctcggcg atacaccgaa aaacgatcac ccttcctaca tctgaattct
     1201 ctgaggcaga tttatgagat cgaaaagatt tgaagcactg gcaaaacgcc cggtcaacca
     1261 ggacggtttc gttaaagagt ggattgaaga aggctttatt gcgatggaaa gcccgaacga
     1321 tcctaaaccg tcaatcaaaa ttgttaacgg cgtcgttacc gaactggatg gaaaacccgc
     1381 cagccagttt gacctgatcg accactttat cgcccgctac ggcatcaatc tcgcgcgcgc
     1441 cgaagaagtc atcgcgatgg attcggtaaa acttgccaat atgttgtgcg atccaaatgt
     1501 caaacgtagc gatatcgttc cccttaccac agcaatgacg cctgcaaaaa tcgtcgaagt
     1561 ggtttcgcag atgaacgtgg tagagatgat gatggcgatg cagaaaatgc gcgcccgtcg
     1621 aacaccatca caacaggctc acgtcaccaa cgtcaaagat aacccagtac aaattgccgc
     1681 cgacgcagct gaaggcgcat ggcgcgggtt tgatgaacag gaaaccaccg ttgccgttgc
     1741 tcgctatgca ccgttcaacg ccatcgccct gctggtcggc tcacaggtgg gacgtccggg
     1801 cgtcttgacc cagtgttcac tggaagaagc caccgaactg aaactcggta tgttaggcca
     1861 cacctgttac gccgaaacaa tttctgtcta tggtacagaa cctgttttca ccgatggtga
     1921 tgataccccg tggtcaaaag ggtttctcgc gtcatcctat gcttctcgtg gcttaaaaat
     1981 gcgctttact tccggctccg gctctgaagt acagatgggt tacgcggaag gcaaatcaat
     2041 gctttacctc gaggctcgtt gcatttacat caccaaagcc gcaggcgttc agggtctgca
     2101 gaatggttcg gtaagctgca tcggtgttcc atcagctgtg ccatcaggca tccgcgcagt
     2161 attagcggaa aacttaattt gctcatcgct ggatctggaa tgcgcctcca gtaatgacca
     2221 gacctttacc cactcggata tgcgccgtac cgcacgtttc ctgatgcaat ttctgccagg
     2281 caccgacttt atctcttccg gtttttccgc tgtgccgaac tacgacaaca tgtttgctgg
     2341 ttcaaacgaa gatgctgaag acttcgatga ttacaacgtc attcagcgtg acctgaaggt
     2401 tgatggtggg ctgcgaccag tacgcgaaga agatgttatc gctatccgca ataaagccgc
     2461 tcgcgcttta caggctgtct ttgccggtat gggactaccg cctattaccg atgaagaagt
     2521 cgaagcggca acctatgccc acggttcaaa agatatgcct gagcgcaata ttgtggaaga
     2581 catcaagttc gcccaggaga ttatcaataa aaaccggaac agcctggaag tggtgaaagc
     2641 cttggcgcaa ggcggtttta ccgatgtcgc ccaggacatg ctcaacatgc aaaaagcaaa
     2701 actgaccggc gattatctcc atacctcagc catcatcgtc gatgacggac aagtgctctc
     2761 tgcggtcaat gacgtcaatg attacgccgg accggctaca ggttaccgcc tgcaagggga
     2821 acgctgggaa gagatcaaaa atattcccgg tgcacttgat cccaacgaaa ttgactaagg
     2881 gtgagaaatg gaaattaacg aaaaattact gcgtcagatt atcgaagacg tgctggcaga
     2941 aatgcaaccc agcgacaagt ccgtttcatt tcgcgcacct gtctctgcaa ctgtcccttc
     3001 cgcgccagat accggaaatt ttctgaccga aattggcgaa gcccaacaag gcactcagca
     3061 agatgaagtc attattgctg taggcccggc gttcggcctg gcgcaaaccg tcaatatcat
     3121 cggtattccg cataaaaaca tcttgcgcga agtgattgcc ggtatcgaag aagaaggcat
     3181 taaagcccgt gtaattcgct gctttaaatc gtccgatgtc gcattcgtag cagtcgaagg
     3241 cgatcgtctt agtggttcag gtattgctat cggtattcag tcgaaaggca caacggttat
     3301 ccaccagcag ggacttcctc ccttgtctaa cctggaactt ttcccacaag cgcctttgtt
     3361 gacgctggat acttatcgtc agattggcaa aaacgccgcg cgttatgcga agcgcgagtc
     3421 tccacaaccg gtgccgaccc tcaacgatca aatggcgcgg ccgaaatacc aggccaaatc
     3481 tgccattttg catattaaag agacgaagta cgtggtcact ggcaaaaaac cgcaagaact
     3541 gcgcgtgacg ttttaacaaa ggaaaccttc atgaataccg atgcaattga gtcgatggtc
     3601 agggacgtgt tgaaccgcat gaacagttta caggatgcag ctcctgtttc tgcggtcccc
     3661 aacgcatcaa tactgagcgc caaagtgacg gattacccgc tggcaaataa acatccggaa
     3721 tgggttaaaa cagcgaccaa taaaacgctc gacgatttca ctctggaaaa tgtgctaagc
     3781 gataacgtca ccgccctgga tatgcgcatt acccctgaaa cattgcgtat tcaggctgcg
     3841 attgccagag atgctggtcg cgatcgtctg gcgatgaatt ttgaacgcgc cgctgagctt
     3901 acctcagtac cagatgatcg catcctcgaa atctacaatg cgttacgccc ttatcgttca
     3961 actaagcaag aacttattgc cattgctgac gatcttgaac agcgttatca ggccaaaatt
     4021 tgtgctgcat ttgtccgtga agctgcggaa ctttatgtcg agcgcaaaaa actgaaaggc
     4081 gacgattaag gacgaactat gcgatatata gcaggcattg atatcggcaa ctcatcaaca
     4141 gaggttgcgc tggcaacgct cagtgcaact ggagagttgt cgttcgtgag tagcgccctg
     4201 gaggaaacca ccggaatcaa aggtacgttg cgtaacgtcc atggtattca ggaggcgctc
     4261 gcacaggcaa cgaaaaaagt cggcatcaat gttagcgata tttcgcttat tcgtatcaac
     4321 gaagcaactc cggtcattgg tgatgtggcg atggaaacca tcactgaaac tatcatcacc
     4381 gaatcaacca tgatcggtca caacccgaaa actccgggcg gagtggggct gggcgtaggg
     4441 ttcaccatca ctccgcaaga gttattaaca cgcccggcgg acacacctta catcctggtg
     4501 gtgtcatcgg catttgattt tgccgatatc gcgacgatga taaacgcctc cgtacgcgcc
     4561 ggatatcagc tgactggcgt tattctgcaa cgcgacgatg gcgtactggt caataaccgc
     4621 ctggaaattc ctttacctat tgtggatgaa gtcctctaca tcgatcgtat tccgctcggc
     4681 atgctggcag caattgaagt tgccgttccc gggaaagtta tcgaaaccct gtctaacccg
     4741 tatgggattg ccaccgtatt cgccctcaat gctgaagaga ctaaaaatat tgttcctgtc
     4801 gcgcgggcgc taatcggcaa ccgttcagca gtggtagtga aaacgccctc tggcgacgtt
     4861 aaagcccgtt cgatacccgc cggaaatatc gaactgcttt ctgccgggcg cacaacgcgc
     4921 gttgatgtag ctgctggtgc cgatgccatt atgaaagcgg tcggtgagtg cccaaaactg
     4981 gaaaacgtga cgggagaacc aggcaccaac atcggcggaa tgctggaaca tgttcgccag
     5041 accatggcgg aactgaccaa caaacccagc aacgaaattt ttattcagga tttgctggcg
     5101 atagatacct ctgtccccgt cagcgtaacc ggtggtctgg caggtgaatt ttcactggaa
     5161 caggctgtgg gtattgcctc tatggtgaaa tccgaccgtc tgcaaatggc gatgatcgcc
     5221 agtgaaatta agcaaaaact gcatgttgat gttcaggtcg gaggcgcaga agcagaagcc
     5281 gccattcagg gggccttgac tacccctgga accacacgtc cactggcaat tctggactta
     5341 ggtgcaggct ctaccgatgc ctccatcatc aatcagagtg gggaaatcgt tgccactcac
     5401 cttgcgggag cgggcgacat ggtaacgatg attatcgctc gtgagctggg cttaaatgat
     5461 cgttatctgg cagaagaaat caaaaagtac ccgttagcaa aagtagaaag cctgttccac
     5521 ttacgccatg aagacggtag cgttcagttc ttccctactc ccctatcccc ccatgtattt
     5581 gcccgcgtct gtgtagtgaa acccgacgaa ctggtgccta ttcccggtga tttgacgctg
     5641 gaaaaagtac gcgctgttcg tcgcagtgcg aaagagcgtg tcttcgtaac caatgccctg
     5701 cgcgcgctac gtcaggtaag ccctgccgga aatattcgcg acattccgtt cgtcgttctg
     5761 gtgggtggtt cttcgctgga tttcgaagta ccgcaattgg tcaccgacgc gctggcacat
     5821 tatcgactgg tcgccgggcg ggggaatatc cgtggtagcg aaggtccgcg caacgcggta
     5881 gctactgggc tactcatcgc ctggcacaag gagtcgattc atggaaagta aagagcgtgc
     5941 accagccatc gttgtcatgg agatcggtga ctgtatcact acatgggatg aggtgcttct
     6001 tggcattgaa gaggaaggta ttccctttcg cattcaacac attccgtcag gtgaagttat
     6061 cgatagcgcc tggctggctg ctcgtcaatc tcccctgttg gttggcattg cctgcgaccg
     6121 ggaagaactg attgtgcatt acaaaaactt accggcatca gcgccactgt ttacgctgac
     6181 gtatcaacaa gataaccata cccggcgcag catcggcact aacgctgccc gcctggtaaa
     6241 aggaattccc ttccgggaat tccactcata atccacagga gaaacgctgt atgaacaacg
     6301 cactaggact ggttgaaaca aaagggttag ttggcgcaat tgaagctgca gatgccatgg
     6361 ttaaatccgc caacgtgcag ctggttggct atgaaaagat tggttcgggt ctgattacgg
     6421 tgatggtccg cggtgatgtt ggcgcagtaa aggccgccgt tgatgcgggg agcgctgcgg
     6481 caagcgccgt tggcgaagtg aaatcctgcc atgtcatccc gcgcccacac agtgatgttg
     6541 aggctatttt acctaaatcg gcataacccc atttgctaag gagcacagcg tgaagcaatc
     6601 actgggatta cttgaagcca gtggtctggc tctcgccatc agttgcgcag acgctatggc
     6661 aaaagccgcg tccatcacgc tggttggcct tgagaagacc aatggctcag gctggacgct
     6721 catcaaaatc accggtgacg tcgcctcggt taatgcggcg atcgcgacgg gagccagttt
     6781 cgccggacag cgaaatggcc ttgtcgcaca caaagtgatt gcccgcccgg gagacggcat
     6841 tctgccccac acaactgaag cattgaccgt gacggttgaa acaagcaaaa caccagcaac
     6901 aaccgtcacc tcagagatta tcagttgtaa tttgtgtctt gacccggcat gtcagcggca
     6961 aaaaggtgag ccccgctcac tttgcctgta cgccaataag cgaggggagt agcagtggat
     7021 aaacaaacct tgcaggaaac agtgagccag attctggatg agatgcgcga gcgccctatc
     7081 ccactgggcg tttccagtcg ccatatacat ctttcatcag cggattattt gcgtcttttt
     7141 cctgaacaac cgattagcga gaaaaagccg ttaatgcaac ctggccaata cgctgctgaa
     7201 caggttgtta cgctggaggg accaaaaggc aaattgcaca acgttcgctt gttggggccg
     7261 ctacgcaatg caagccaggt ggaaatttcc cggacggacg ccagaacgtt agggatcacc
     7321 gccccgctac gcatgtcagg cgatctcagc ggcactccgg ggattcgttt gataagccca
     7381 tttgcggaac tggagatcac atctggcgtc attgtcgcgc aacggcatat tcatatgtct
     7441 ccgctggatg ccctgattta tggcgttacg catggcgata aagtggcggt tgccattgaa
     7501 ggtgatgagc gacgactgat attcgaaaac gtcgctgttc gcgtctctcc ggatatgcgt
     7561 cttgaaatgc atattgatac cgatgaggcc aacgccgcag gcgctgatag ccctgacgtt
     7621 tttgccacac tggtgtcagt gccatgaata acatgctgat gcaacgtatt gttgacgaag
     7681 tggtttttcg ccttaaacaa cgtgcgggaa aaacactcgt gctgactgtt tttcaattgc
     7741 gagacgccag cgttcaagag agcgttcatc agtatgcgtc attacaaatc cgatatgtcg
     7801 atctgccgct gctgcgacaa cttgcggaga acgaaacgtc agatcgagca tctatccaga
     7861 tacacgaagc gttagcctgg ggaatgcaca ttcaattgtc gttgcagcgt catttcctga
     7921 acgccatcga attgaagact ctcgcccgtc taccgctaag ctggtgcgac gaacaagggc
     7981 agccgatcta ccttcatcat aatcgacttt taagttatgc cgatatcgca cagttaagct
     8041 cgggaatact ggtgttacag cggaaatgtt gtgtgaccgc actggctcgt gaagcggcaa
     8101 ttacccgaaa tattcagtta atcaggcagg agtaacctat gcatctggct cgagtcactg
     8161 gcgcggtagt atccacgcaa aaatcacctt ctctgatcgg gaaaaaactg ctgctggttc
     8221 gccgggtcag tgctgacggc gaactgcctc tcacgccatt aaccggcgac gaagttgccg
     8281 ttgattcagt cggcgcaggc gttggtgagt tggtactcct cagcagtggt tccagcgcca
     8341 gacatgtgtt ttcaggccct aacgaggcca tcgacctggc agtggtcggc attgtcgata
     8401 cgctttcgcg ctaaggagct aaaagatggc aatttatacc cgaacaggtg attcaggaag
     8461 tacgtcttta tttaccggac agcgcgtcag caaaactcat ctacgtgtcg aaacctatgg
     8521 cacgctggat gaactgaacg cgacactcag tctgtgttat tgcgccaccg ccatcgaaag
     8581 ccatcgcatc ttactggaag ccattcaaca acagatattt tggtttagcg ccgaactcgc
     8641 cagtgaaagc gaacagccgt cagcgcaaca gcgttacatc ggtacggaag aaattgccgc
     8701 gttggaaaac gcgatcgata gtgcaatgaa cgccgtcccc ccagttcaca gcttcattct
     8761 gcctggtcga tgtgaagccg caagccgcat gcatttcgcc cgcacggtgg ctcgacgggc
     8821 tgaaagacgg ctggttgaac tgacaacgga aaccaccgtc cggaatgttt tgctgcacta
     8881 tatcaaccga ctttccgatt gcctgtatgc cctcgcccgc gtggaagaca acgttgctca
     8941 tcaaaacctg atgattcagg aaatcacaaa acgttatcac gaagccaacc acacaccagc
     9001 attgaaggaa cgcacaatgc cgctcacttt tcaggatctt caccagctca ttcgtagtgc
     9061 ggcaatgcgc gcggatgaac tgcatattcc cgttgtcatc agcattgttg atgccaacgg
     9121 aacagaaagt gttacctggc ggatgcctga tgccctgttg gtcagcagtg aactggcacc
     9181 gaaaaaagcc tggaccgccg tggcgatgaa aacggcaacc cacaagcttg ctgataccgt
     9241 gcaacccgac gcgccgcttt acgggctcga aagccatatg caaggaaaag tcgtcacttt
     9301 tggtggcggc ttccccctct ggcgtgacgg aaaattgctt ggcgggcttg gcatcagcgg
     9361 cggtagcgtt gaacaagaca tggatattgc ccaaagcgca atggcggcaa ttaacgtggg
     9421 agtaaaccaa tgaataactc tgaattagaa acactgatcc gcaatattct gcgtgagcaa
     9481 cttgtccccg ctacaccacg aacgcaaaga aacgctattt tccagactgt cgatgaagcg
     9541 gtctgcgcag cacactaggc ctttttacgc tttcaacagt gcccgcttaa aacccgtagc
     9601 gccattatca gcgccattcg cgaagaatta atgccccgcc ttaccgaact ggcagaagaa
     9661 agcgccaaag aaacggggat gggaaataaa gaagataagt acctaaaaaa caaagcggca
     9721 ctcgataaca cgccaggaat tgaagattta accaccacag cactaaccgg tgatggtggc
     9781 atggttttgt ttgagtactc tccgtttggg gtgattggct cggtcacgcc aagcaccaac
     9841 cccacggaaa ccatcattaa caacagcatc agcatgctgg cagcaggtaa tagcgtctac
     9901 ttcagtccac acccgggtgc caaaaacgtt tcgttaaaac tgattaccac aatcgaagac
     9961 atcgcgtttc gctgtagtgg cattcataac ttgatcgtta ccgtatctga accgaccttt
    10021 gctgccaccg aacagatgat ggcgcaccct aaaatcagcg ttctggcgat taccggcggt
    10081 ccggggatcg tcgccatggg aatgaaaagt ggcaagaaag tgattggtgc cggagcgggt
    10141 aaccctccgt gcatcgtcga tgaaacggcg gatattgtga aagccgccga ggacatcatc
    10201 aacggcgctt catttgatta caacctgccg tgtattgctg aaaaaagcct gattgtcgtg
    10261 gcatcagtgg cagattattt aattcagcag atgcaaagtt ttggcgcact tcttcttaac
    10321 tatgagcaaa ctgagaaatt gcgtgcaatt tgcctgccag acggtagcgc caacaaaaaa
    10381 ctggttggga aaagcccatc agccttgctg gaagcggcag gcctcccttt gcccgcaaaa
    10441 gcgccgcgcc tactgattgc tgttgtcgac gccgatgata gttgggttac ttgtgagcaa
    10501 ctaatgccaa tgctgcccat tgtgaaggtc aaggattttg acagcgctct aaccctggcg
    10561 ctgaaagtgg aagacggctt gcaccacacg gcaattatgc actctcagaa cgtctcacga
    10621 ctcaaccttg ccgcgcgcgt tatgcaaacc tcaatatttg tcaaaaacgg cccctcctgg
    10681 gcgggtattg gcgtcggcgg cgaaggcttt acgaccttta ccattgcaac accgactgga
    10741 gagggtacaa cgtcagcacg gacttttgcc cgctcacgac gctgcgtgtt aaccagcggc
    10801 ttttcaattc gctagagaaa cggaaatgaa tactttttca ctgcagacgc gtttatacag
    10861 cggaccagga agtctcaagg tacttagccg tttctctcat agacacatct ggattatttg
    10921 cgatgccttt ctggcacgct caccacttat tgacacgttg catcaggcgc tgcccgacag
    10981 taatcgtctc agtattttta gcgaaataac gcccgcccca actatccaga ccgtggtgaa
    11041 aggcattgcg caaatgcaga ctttacgccc ggatgtagtt atcggttttg gcggcggttc
    11101 tgccctggac gccgcaaaag caattgtctg gttcggtcgc cagtgtggca ttgaaattga
    11161 aacctgcgta gccattccta ccaccagcgg tatcggttcc gaagtgacca gtgcttgtgt
    11221 catcagcgat cctgagaaag ggattaaata tccccttttc gataatgcac tttatccgga
    11281 tatcgcgatc cttgatccat ctttggtcgt cagcgtgcca ccggcgataa cggccaatac
    11341 cgggatggat gttttaaccc atgcgcttga agcttatgtt tctcctcgcg ccagcgactt
    11401 cacggatgca ctggtcgaaa aagccgtgca aattgttttt caatacctgc ccactgcagt
    11461 gaaaaaaggc gattgtcttg ccacccgtgg caaaatgcat aatgcatcga cgcttgcagg
    11521 aatggccttt agccaggccg gactcggttt aaaccatgca attgctcatc aactcggcgg
    11581 acagtttcat ctgccgcatg gactggcgaa tgcgctcctg ctcacggcag ttatccgctt
    11641 taatgctggc gatccgcgca cagccaaacg ctatgcccgg ctggcgaaaa cgtgccattt
    11701 atgcccggac aacgccaatg acaccgccag cctgaatgca cttattcaac atattgagca
    11761 gcttaataca acctgtacgc tgccgactct tgccaatgcg ctgaaagaaa aaaaagcaga
    11821 atggtcaata cgcataccag atatggttca ggcggcgctg gcggatgcaa cgttgcgtac
    11881 caacccgcgt gcagctgatg cctccgcgat tgcagaactg ctcgaggagt tgttatgaac
    11941 gtcggattaa tctcctgcga cgaccagacc atccgcgagc gagtacaagc agcaggtgtc
    12001 gtgggtgctg gcggcgcagg ttttccaacc catattaagc tacaagcgcg tgctgacact
    12061 ttcctggtga atgcggcgga atgcgaaccc atgttgaaag tagatcaaca gttaatggcg
    12121 cagcaagcca gccgcctgct tcgcggtgtt cactatgcga tgaaagccac cggggccagt
    12181 agcggaatca tcgcgctgaa agaaaaatat caacgggcaa taaacgcact cacaccactg
    12241 ttaccaccgg atatccgcct gcatattttg cccgacgttt accccgcagg agacgaagtg
    12301 ctgaccatct ggatggcaac tggtcgccgc gttccacccg ctgcattacc cgttagcgtc
    12361 ggtgtggtgg tcaataatgt tcaaaccgta cttaatattg ccagagccgt cgagcaacag
    12421 tatccagtga cgcgccgcac actcaccgtt aatggtgcag tggcgaagcc aataacggta
    12481 accgtcccca tcggcatgtc tttacgtgaa gtcctggcgc tggcgggcgg cgcaacagtg
    12541 gatgctcctg gttttatcaa tggcgggccg atgatgggaa gtttgatcac atcgctcgac
    12601 acaccagtca gcaaaaccac tggcggcttg ctggttttac ccaactctca ttcactgatc
    12661 caacgtcgaa tgcagaatga ccgcagcgta cttgccgtcg cgaaaactgt ttgtgagcaa
    12721 tgccgcttgt gtaccgatct ctgcccacgt catttaatcg gtcacgaact ggcaccccat
    12781 ttattagtac gtgcggttaa ctaccaacag ctcgccacgc cgcaactgtt actgacagcg
    12841 ctcacctgtt cagagtgcaa cgtctgcgcc agtgtcgcct gtccggtagg aatttcacca
    12901 atgcgcataa accgcctgct taagcaggaa ttacgcgcac aaaatctccg ctacgaaggt
    12961 gccttaaatc ctgcagatcc catggcaaat taccgactga ttcccgtaaa acggctggta
    13021 accagacttg gcttaacccc gtggtatcag gatgcgccat tgtctgaaca ggtgcctcag
    13081 cccgaaaaag tgacgttatt gttacgccag cacatcggtg ccagcgcaat tgcgtgtgta
    13141 caaaaaggcg atcgcgtcgt tcatgggcaa tgtgtaggtc aaattccaca cggcacatta
    13201 ggtgccccca ttcatgccag tattgatggc atggtcagcg atgtgacaga aaacgcgatt
    13261 acgcttgtga gaggttaacc atgtcgaaag cggtaggtat tttagaactt agcagtatcg
    13321 cgaaagggat ggaaaccggc gacgccatgc tgaaaagcgc caacgttgag ttactggtca
    13381 gcaaaacgct atgccccgga aaatttctgc tgatgctcgg tggcgatgtg ggcgcggtgc
    13441 aacaggccat caacagcgga gcgtcccttg ctggtgaaat gctggtcgac agtcttgtcc
    13501 tgccgaatat tcatcccagc ctcctttctg caatcagcgg acttaaccat gttgaacagc
    13561 accgggctgt cggcgtagtc gaaacctgga gtgtcgccgc ctgcattgac gctgccgatt
    13621 gtgctgtcaa agctgcaaat gtcattcttg ttcgcgtaca tatggctttt ggcattggcg
    13681 gtaaatgcta catggtggtt gctggcgata tttcagatgt taataacgcg gtctctgccg
    13741 ccagcgtgcg cgcgggggaa aaaggtttgt tggtctatcg gtcggtgatt gcccgtccac
    13801 atgaagccat gtggcgtcag atggtggagg gataatgaca actcagcaca caactgaacg
    13861 gatgattcag gaatatgtcc caggtaagca ggtcacgctg gcacatgtca tcgccaatcc
    13921 agggaaggat ctgtttaaga aattgggttt accagacacc atttccgcta ttggtattct
    13981 gacaattacc cccagcgaag catctatcat cgcatgcgat atcgccacaa aatcaggcgc
    14041 tgtagagatc ggttttcttg atcgttttac cggtgccgta gtgctgactg gtgatgtttc
    14101 tgcgatcgaa tatgcactta gccaggttac ccgtacgctg ggtgaaatga tgcgctttac
    14161 cgcctgcccc attacgcgga cctgacgtta tgcaacgtat gatgctcatc ggccccagcc
    14221 agtgcggaaa aacgtcactc acgcaatgtc tgcgtggcga aaatattcat tatcagaaaa
    14281 cgcaggcaat cgtctggtcc ccgaccacca ttgatacacc aggcgaatac ctcgaaaacc
    14341 gcagcctcta cagtgcactg ttggtcagtg cctgcgaagc ggacattatc gcgctggtgc
    14401 ttaatgcgga cgcaccgtgg tcaccttttt cgccaggctt tacctcgtca atgaatcgcc
    14461 cggtcattgg agtagtaacc aaagcagatt tagccaacat ggaacaaata tctttggtga
    14521 aatgttggtt acgggaagct ggagcgcaca acgtgttagt aaccagtgcg gttaataacc
    14581 atggagttgc agaactttta gccttgctgc atacagaaga aggctgtcgc taatttcgac
    14641 aggttatcag atgggaatga tgacggtgat acacagaaaa gaatcaatga gcaatgaggg
    14701 ttgccgggca accctcattg aataaaacag gaattacttc gcttcgccgt ttttcatttc
    14761 gcccatagct ttcagctttt tggagatatc gcggcgttct ttggagagct cggcattttt
    14821 gatgatgtaa tcgtcaacgc gatcttcata gtcacctttc atgctggcga tgatgccctg
    14881 gattgcttca acgctcatcc caggcttgat gtaatcgctc aggttgtcca gcagcaggac
    14941 gcgcttctgg ttgtcacgga tctttttctc aacgtcctga atttcacgtt gcagtttgtt
    15001 cttacgacgg aacagacgaa caaattccag tacgtcctgg aatgaaggct tggtagtttc
    15061 catttttata cccctgataa tgtgagagtc agattcgttt aagcaaccgc tattcgttag
    15121 ggccaacatt actcatagct gccgcagatg acaatgcttt tatccctttt cactatcata
    15181 ccctttatcc ttgctgaatc gaagcagcag caagatgatt ctgaagttca ggaattattt
    15241 gcgcaaggcc cggcaaatca gattcgatag caactcaagc tgatgcgccg tttccatgtt
    15301 cagccacaca taaccataga ttggtgtttc cactaccttc aggtcatggt ggttattgag
    15361 caactgacgc agctcttcca cagcatcgtt caatttttcc gtattggcaa aaaccggctg
    15421 cggattacct tcgtacagcg catgaacaaa gctcagcagt atttgctgca tcatgtgctg
    15481 ggtatcacga agtttttgcg cgttcaataa cacgaaatgg ctggggcgcg tggcccagta
    15541 cgcattgatt tgcaactcca gcatacaaac cagattgcgg ttaatggtct ggataccttc
    15601 atatatcgat tttggaatac gggtttcttt gctggcgggc gcaatcagcc cacgcatttt
    15661 cacggcatcg gtcaggagtt tttgtagatg gctttccaga cgtgggcgtt cgagtaagtt
    15721 cggtgagaat gcagattgat agacccgatt atactcggtc agacttttcg ccagttgaat
    15781 gcgccagtgg atgaacgccc gttgtggcca gataccggta aacaacattg ccagtaaaga
    15841 gccgaggatc acatcgccgc ttcgccataa cgccgtatca atttcacctg tcggggaacc
    15901 aacaacaatt gccagcgtca ccccaatcaa taatccttga tacggtttct tgcccagcgc
    15961 cagccaaccg caaagaaaca tcgccgccgc gcaccagact aacatcagcg gtaacgaaat
    16021 taactccagt tgcagagcga taagacctaa aatcgaaccc aacaccgtac cgccaatacg
    16081 ttcgaaggca cgagggacaa cgttacccca gaacgagatt ggccccataa tcaccaccat
    16141 ggtgaccagc ggccaggtgc tttccgggat agtaaacagg cggataatga gaaaagtgag
    16201 caggaatgcc agcgcgaccc gagtaccatg cactatgcgg taatggcggt atacccggat
    16261 ttcaaacggg cttaatgact tatcggcacg cacgcagaaa ctccgtaatg aagattaaaa
    16321 gcgctcataa ctcatctgtc ggccagaagg cgcgcctctg aattatccga cggacaaacc
    16381 gggaacagtg taatttcccg gcaggagcga aaaaattgaa aggcgcatct tactcttttc
    16441 gctttcaaaa aaagagtggt catcgcgtta acacaccgcc ctgagatgaa ttagtgatgt
    16501 tttggctgca ccgcaacata catttcgata tcccagtacc catcttccgc gccattgttc
    16561 aaataaacct cgaagcaggg ctttggtaac atttcataag cactgtcctg caagaggcta
    16621 ttaaagaact gataccaggg tttagcaaaa tcatcaccga ctacacgagc caccgccacc
    16681 gcatactgac cacctgaaat ttctgtcaga atgacgccct cactgttttc gggaagcgta
    16741 aagttattcg gtaccgtcac aacggtgtcg cagcgtaatt tttcggcggg tgtttcatct
    16801 ggattgtcgt aatagacagc aacccactcc ttcggcacaa tatttttgtt atctacccac
    16861 atcatcaact gctcaaagcc tttctttacc gtctgttccc acgggccaac gagatggaaa
    16921 cctgcaacgg tacgtttctc ttcctgcgta atctcgtagt tcatgacgcc tccattaaca
    16981 ctgtttttat atacagcata gttgcaatat taaaaccaca aggaatgagt gttgattatg
    17041 cgagcagact cgcactcctg ccagtctgct gcaaaagaaa ggtcaggcct tatggtggaa
    17101 ataatcactc aggcgggaaa acatgccgcc ttccccgaca gattccaggg taaccagcgg
    17161 ccagtgcgcc acctgtttat cacggtcgta aagttcaatt tcccctaccc gctgatgggc
    17221 gctaattggc gcggtgagct ctttaccatc aagggtatat ttggctttga tatgtggaat
    17281 ttcggctttc ggtagcacca tccagaactc ttgttccgtt cctaggtcga tattttcttt
    17341 atcgccatac cagatgcgtt ccgttccgac ctttttccca cggtgcaaaa tttgcaccgt
    17401 agtaaagttt tgttgccccc aacgcagtaa ttttcttgcc tcttcctcac gaccttttgc
    17461 actgtcagcc cccattacca ctgcaatgag acgacgctgc ccatctacag ccgaagcaat
    17521 gagattaaac ccggcaccag aagtatgacc cgttttcagg ccgtcaacat tcatggtttt
    17581 atcccacaac aacccgttac ggttttgctg ggtgataccg ttccaggtga gacttttctc
    17641 actgtacata tgataaaact cgggctcgcc gtggatgata gcgcgagaaa gcacagctaa
    17701 atcataagcc gagctatgct ggccaggtgc atccagacca tgcactgttt caaaatgcgt
    17761 atccttgaga tgcagcttct cggcatagtt gttcatcatt tcaacaaact gccgttgccc
    17821 accggcaata tagtcagcca gagcaacaca agcgtcattt ccggaatcca caattaaacc
    17881 acggcttaaa tcacgtaccg atacgcgatc gccctctttc aaaaacatca gtgaagaacc
    17941 gacaaacacc ggattatctt tcgcccacgc atcgcgcccc acggtgacaa tatcgtctgg
    18001 cgtaatgcga tgactatcga tagcgcgatc cacgacataa cccgtcatca gctttgtcag
    18061 gctggcggga ttgcgctgtt gatgctcatt acccgcggtg aggatctgac cggtggtgta
    18121 atccatcaat acccaggacc cggcatgaat ctctggaggc tgaggtgaaa aaggaatgtt
    18181 ttccgccgca aaaccagacg ataagttaaa aacgaacaaa gaagcagcaa taataagacg
    18241 gcgtttcaac agcaaaccct caggagtttc aaatagctgt tctttttacg gaaatactta
    18301 tgaactggct ggaataaagt gcaagaaaat gtgactaccc tctcattttt atctgacatg
    18361 atctgttgcc actcgctgcc aaattgtggc gctaaagctg attagcacgg tgatatttga
    18421 tactctggca gacagcagaa ataacggatt taacctaatg atgaatgacg gtaagcaaca
    18481 atctaccttt ttgtttcacg attacgaaac ctttggcacg caccccgcgt tagatcgccc
    18541 tgcacagttc gcagccattc gcaccgatag cgaattcaat gtcatcggcg aacccgaagt
    18601 cttttactgc aagcccgctg atgactattt accccagcca ggagccgtat taattaccgg
    18661 tattaccccg caggaagcac gggcgaaagg agaaaacgaa gccgcgtttg ccgcccgtat
    18721 tcactcgctt tttaccgtac cgaagacctg tattctgggc tacaacaatg tgcgtttcga
    18781 cgacgaagtc acacgcaacg ttttttatcg taatttctac gatccttacg cctggagctg
    18841 gcagcatgat aactcgcgct gggatttgct ggatgttatg cgtgcctgtt atgccctgcg
    18901 cccggaagga ataaactggc ctgaaaatga tgacggtcta ccgagctttc gccttgagca
    18961 tttaaccaaa gcgaatggaa ttgaacatag caacgcccac gatgcgatgg ctgatgtgta
    19021 cgccactatt gcgatggcga aactggtaaa aacgcgtcag ccacgtctgt ttgattatct
    19081 ctttacccat cgtaataaac acaaactgat ggcgttgatt gatgttccgc agatgaaacc
    19141 cctggttcac gtttccggaa tgtttggggc atggcgcggc aataccagct gggtggcacc
    19201 gctggcgtgg catccagaaa atcgcaatgc cgtaattatg gtggatttgg caggagacat
    19261 ttcgccatta ctggaactgg atagcgacac attgcgcgag cgtttatata ctgcaaaagc
    19321 cgatcttggc gataacgccg ccgttccggt taagctggtg catatcaata aatgtccggt
    19381 gctggcccag gcgaatacgc tacgcccgga agatgccgac cgactgggaa ttaatcgtca
    19441 gcattgcctc gataacctga aaattctgcg tgaaaatccg caagtgcgcg aaaaagtggt
    19501 ggcgatattc gcggaagccg aaccgtttac gccttcagat aacgtggatg cacagcttta
    19561 taacggcttt ttcagtgacg cagatcgtgc agcaatgaaa attgtgctgg aaaccgagcc
    19621 gcgtaattta ccggcactgg atatcacttt tgtcgataaa cggattgaaa agctgttgtt
    19681 caattatcgg gcacgcaact tcccggggac gctggattat gccgagcagc aacgctggct
    19741 ggagcaccgc cgccaggtct tcacgcccga gtttttacaa ggttatgcag atgaattgca
    19801 gatgctggca caacaatatg ccgataacaa agagaaagtg gcgctgttaa aagcgctttg
    19861 gcagtacgcg gaagagatcg tctaatttga agcctccgcc gctggtacgg cggaggtcaa
    19921 tgttgagtca ggctttttga acggtgatac tccacgctgc atcgccaatc tgctgataat
    19981 cggtgatggc atgcccttct tctgccgccc actgtgggat tgcttcagtt gcctgggtgc
    20041 aatcaaactc aattaccaac tcatcgccgc tggccatttc tgccagtgct gcttttgctt
    20101 ctatgagtgg aaacggacag acctgcgtca ccacatccag ctttttaatc gccatcttta
    20161 caccttactt aatttgccgc agcagttgcc agtcgcgcct tacgctgcgg tcgaacatac
    20221 acaagccagg acgcagtcca gactccgaga atcataaata caaggccaat ccagccctgc
    20281 caggtcatca ttgcagtcat aaccagtcca ttaccgataa agcaaccacc tgcaatactg
    20341 gcaccgaagc ccatcagtac accacctaac ccgctacgta atgttgtttg tgcatcagct
    20401 gcgcgaacgc ggaactcacg gctcgctttg gctgcaataa atgaccccac gaagatccct
    20461 aacaccagga aaacgcccca gttaatgtat ttcacgtcac ccgcgaccag aaattgcaga
    20521 atattggccg ttggggaagt gattcctaac ccaaacatgc gtccggttgc ttcactcagg
    20581 ggccaggcta aaagcgcaat caaaccgatg agtacagcag tgacaaaggg atgccagcgt
    20641 ttttcaaaca gaatatgagc gatcccagtt cggcgcggcg gtaaggtcgc gactttgagt
    20701 tttggcttct tcaactcttt catcaccacc cagagcgtta tcaccagcaa aacggcaacc
    20761 aacggccaaa cagacaaatt aaaagtctca gcaatagagt tatgttcagt actgtagtgc
    20821 tgcaaggttt gatttaaacc actggcatgt ggagaacgca tcaccgcact catcaccata
    20881 taagtgaaaa gcgcgatcca actgccgatc aatccctcac ccgcgcgata ccaggtcccg
    20941 gtggcacatc cacccgccag aacaattccc agcccgaaga tatacccacc tataacagta
    21001 ccaagccacg ggaacgcctc ggcttcgtaa gtcagtaggc ccgcctgaat taacgcaaaa
    21061 acccccacgc tttgtaccga aattgcaatc agcaaggcgt aaaacatgcg attatttttc
    21121 gcgatataca tatcgcgaaa accacctgtc aggcaaaaac gcccacgctg catgacaaat
    21181 cccagcagag caccacaaat tagcccgctt aatatcattg aaaacataaa ttaattaacc
    21241 agatgaatgt taatgaggaa attattcatg actggtggaa tgcagaccaa taaccaaatt
    21301 ctctaaatta gaacaaatgg ttattaatga ggatttcgat aaaaaaaaat aaaaaaaccg
    21361 gagccaaata ttcattcgac tccggttgat tattagaaaa gatgattatg caacgtcttc
    21421 gtactgcgga actgggttac ggaagctctt ggtgacgcaa gccaggtaaa tcagaccgat
    21481 agcagcccaa atcagaccca gaaccattga gctttcttcc aggttaaccc acagcgcacc
    21541 aacggtcagc gcaccacaca tcggcaggaa cagatactgg aagtgatctt tcagcgtctt
    21601 attacgcttc tcacggatcc agaactgcga gatgaccgac aggttaacga aggtgaacgc
    21661 caccaacgca ccaaagttaa tcagcgctgt agccattacc aggtcgaagt tgattgccag
    21721 cagggcaatc gcgccaacca ggatgatgtt catcgccgga gtacgccatt tcgggtgtac
    21781 ataaccgaag aagcttttcg ggaatacgcc gtcacgaccc attacgtaca tcagacgcgc
    21841 tacgcctgca tgcgccgcca taccggacgc cagtacggta atggtggaga agatcagcgc
    21901 gccaacctgg aacgctttac ccgcaacgta cagcatgatt tcaggctgtg acgcatccgg
    21961 atctttaaag cgagagatat ccgggaagta cagctgcagg aagtaagttg caaagatgaa
    22021 gatcatgccg ccaatcagcg cggtcaggaa aatcgcacgc gggatcacgc gctctgcatc
    22081 tttggtttct tccgacaggt tgctgatgcc gtcaaagccg gtaaaggaaa agcacaggat
    22141 tgtcgccccg gtaatcatcg gaataacatg cgcatcgcca gaccagaacg gacgagtgct
    22201 cgccagcgta ccagcgcctt caccttcaaa tacgccataa acaaccatgc ccagaatcac
    22261 cgcgatcagc actacctgca acacgacgat tacagtgttg aagttcgcta cggatttcag
    22321 actacgcagg ttaaaggcgg tcataaaggc caccagcgcc accacaaaca tccacgatgg
    22381 gatggaaggc accagagctt caaaatagat tttcgccagc agaatgttga tcatcggcgc
    22441 gaacagatag tcgagcagag aagaccaacc caccataaag ccgacagtcg ggctaatgga
    22501 tttctgggcg taagtatatg cagagccagc agaaggatag cggcgaacca gcttcccgta
    22561 gctcagagcc gtaaacagga tcgcaatcaa tgcgaacgca taggctgtcg gcacatgacc
    22621 atccgtgagg cctgaaacga taccaaaagt atcaaacagc gtcatcggct gcatataggc
    22681 aagacccatc attacaaccg gaactaacgt aagcgtttta cgcaattcca cgcgagaggt
    22741 gtttggagta acgttatgcg acatggtcat tctcctttac ggtgataacc gtcgcgtaag
    22801 caaaaaattg ccccattttt ttggattcct cagcgacaac aactgtcgat ttttagtaaa
    22861 tatctatccg gtacgaagcc cggcctcttg gtatgaatga ttggtttgaa gcaaaaaata
    22921 accgacgctg atgaaacgtc ggtttttagt cattttttga cagcggcgca ttgtgcctaa
    22981 atggggggga aatgacaaga gaatgagaag cttgtcataa taatttttct ttaaatggct
    23041 gattttccgt catcagattt tgcataaaca ccggcgatcg cactatttgc taaaatttca
    23101 tcccgccacc agccgctggc aagccctgcg gtctgttcgt tccagccaat ccagacgggt
    23161 tcaaagagag tttgggcgac cactttcttc tcgattaacg cgccactatc gagaaaacgt
    23221 tgcaccagat aacggggtaa atagccgcag ccgaggccgc taatttgcag ctccagcttg
    23281 gttttaaaat cgaaaacggt aatggcttct tgttcgtcga gaagttgtga ggccgtagaa
    23341 gcgcctgcct gcgcagtgtc ccccaccaca atcgcccgat atcgcttaat gatacgacgg
    23401 tttaacggct cttcttcctg ggctaacgga tgatgaggcg cgaccgcaaa gacttgttcc
    23461 agatcgccca accgcgagaa accaaactca ctggaagatg gtggctcatg catcgcgcca
    23521 acaataatat ccgctcgccc ctgagtcagg gcatcccagg aaccaccgag tacgccattg
    23581 ataaacttca ggcgcgtcac gctatgatgt tgataaaaag cttcaatcag tggcgcaagc
    23641 agagaaaaag gaaacgtgtc gtccacgcca atcaccagct cgttttccca gccttcatgc
    23701 agtttgatcg cctgcttttc cagttctcgc acggtatgca gaacttcccg ccccttctct
    23761 aataacattt ttccggtacg ggtgaattta gcgcggtgac cgctacgatc aagtaattga
    23821 atattaaggt cgctttccag cttgtgaacg gtgtaactca gagcggacgg cgtcttgtag
    23881 agtttagccg acgccgccgc aaaactgcct tctttttcta aggcatcgag gatcatcaac
    23941 acatccagca gttgtttcat acacgccccc ttgcagtgca tgcggcgtcc tgctggcgtg
    24001 atcgtttact ccagcggcat taccagcgga tcgggatact ggtattcaaa tcccagttca
    24061 ttacaaatcc gactgccatc aataatctta cctttgccgc tgtccagact atttctgaac
    24121 tgcggaggtt ccagccccag taaacgggcc atctgcggat agaaaacatt acgcgcaggg
    24181 tgagctggcg cacatatatt atagatgtgt ccgcctttag gtgcctgtaa caacagagtg
    24241 atagcgccaa taacatcttc taaatggact aaattaacac catgttcacc atcaggcgcg
    24301 gtttttccgg caaagaagcg tccgggatga cgtcccggtc cgaccagccc cgcaagacgc
    24361 agaatatcga ccgaagtacc gggtaaattg tgcagccagt cttcgagttc ttctaatact
    24421 cgtccactgt tggtcaccgg attacgcggg gtggtttctt tcaccgtgcc ttgcgcgtcg
    24481 ccatagacag acgttgagct ggtaaaaata atgcgaggaa tacgatgggc cagcgcgcta
    24541 tccactaact cttgtaccgc ttgtaaatag aactcatcgc cggggccgct acgacgtgcc
    24601 ggaagcgtaa tgaccagcgc atcggcatcc atcagggcat ccagatcgtc agaatcgcaa
    24661 actaactcag gctccatgcg aagcagatag ctatcaatgc cactcattcg ggccgcttcg
    24721 acaccatctt gtgtggtttt actcccggtg acttgccagc ctcgcgctga aagtgacatc
    24781 gccagcggca tgcctaacca ccctaacccg acaattgcga cctttttcat ccgttatctc
    24841 ctgatacacc ttagatctat aaggctacgc tagcgtatca aaactgacaa ttcattctat
    24901 gaatgaatct gttcaataat gataacgaca tgctgcaatg agcagtgaat cgtcggtaac
    24961 cgcgtatacc agacggtgct cctctgtaat gcgtcgggac cagaaacctg acaaattatg
    25021 tttcaggggt tctggcttcc ccttaccttc aaatggcgtt ctgcgggtat ctttgataag
    25081 ttcattgatc tttttaacaa ttcgcttatc tgtttcctgc cagtacagat aatcatccca
    25141 tgattcctca gaccagatta gtttcactca ataatgtcct tttctgttcc tttgcctgat
    25201 ttcaggctat cgattgagtc catcaatctc cgggcgttag cgggggagcg cagtagataa
    25261 gccgtctctt ccagcgagtt gtattcttcg agtgacatca gaacacaagc ctctccattc
    25321 tgacgagtaa taaggatcgg ggcatgatct tcaacggctt tcatcattgt tgccgacaaa
    25381 ttctgacgcg cttcgctgta gctaattgta cgcatgtcaa tctcctcttt tgtacagttt
    25441 attgtacaat gatgggcgtt aattaactat ttattaatta gtttgtagat caaggtattg
    25501 tcagtgagac gaaaatccag gcttcgctat ttttggtgcc gtcagcttag aggacagtcc
    25561 tcttagcccc ctcctttccc cgctcattca ttaaacaaat ccattgccat aaaatatata
    25621 aaaaagccct tgctttctaa cgtgaaagtg gtttaggtta aaagacatca gttgaataaa
    25681 cattcacaga gacttttatg acacgcgttc aatttaaaca ccaccatcat caccatcatc
    25741 ctgactagtc tttcaggcga tgtgtgctgg aagacattca gatcttccag tggtgcatga
    25801 acgcatgaga aagcccccgg aagatcacct tccgggggct tttttattgc gcggttgata
    25861 acggttcaga caggtttaaa gaggaataac aaaatgacag acaacactcg tttacgcata
    25921 gctatgcaga aatccggccg tttaagtgat gactcacgcg aattactggc gcgctgtggc
    25981 attaaaatta atcttcacac ccagcgcctg atcgcgatgg cagaaaacat gccgattgat
    26041 attctgcgcg tgcgtgacga cgacattccc ggtctggtaa tggatggcgt ggtagacctt
    26101 gggattatcg gcgaaaacgt gctggaagaa gagctgctta accgccgcgc ccagggtgaa
    26161 gatccgcgct actttacctt gcgtcgtctg gatttcggcg gctgccgcct ttcgctggca
    26221 acgccggttg atgaagcctg ggacggcccg ctctccttaa acggtaaacg tatcgccacc
    26281 tcttatcctc acctgctcaa gcgttatctc gaccagaaag gcatctcttt taaatcctgc
    26341 ttactgaacg gttctgttga agtcgccccg cgtgccggac tggcggatgc gatttgcgat
    26401 ctggtttcca ccggtgccac gctggaagca aacggcctgc gcgaagtcga agttatctac
    26461 cgctcgaaag cctgccttat ccagcgcgat ggtgaaatgg aagaatccaa acagcagctg
    26521 atcgacaaac tgctgacccg tattcagggt gtgatccagg cgcgcgaatc aaaatacatc
    26581 atgatgcacg caccgaccga acgtctggat gaagtcatcg ccctgctgcc aggtgccgaa
    26641 cgcccaacca ttctgccgct ggcgggtgac caacaacgcg tagcgatgca catggtcagt
    26701 agcgaaaccc tgttctggga aacgatggaa aaactgaaag cgctgggtgc cagttcaatt
    26761 ctggtcctgc cgattgagaa gatgatggag tgatcgccat gagctttaac acaatcattg
    26821 actggaatag ctgtactgcg gagcaacaac gccagctgtt aatgcgcccg gcgatctccg
    26881 cttctgaaag cattacccgc actgttaacg atattctcga taacgtgaaa gcacgcggtg
    26941 atgacgccct gcgggaatac agcgcgaagt ttgataaaac cacggttaac gcgctgaagg
    27001 tgtctgctga ggagattgcc gccgctagcg aacgcctgag cgacgagcta aaacaggcga
    27061 tggcggtggc agtaaaaaat attgaaacct tccacactgc gcaaaaactg ccgccggtag
    27121 atgtagaaac gcagccaggc gtgcgttgcc agcaggtcac gcgcccggtc gcttcagttg
    27181 ggttgtatat tcctggcggc tccgccccgc tcttctcaac ggtattaatg ctggcgactc
    27241 cggcgcgtat tgcgggctgt aaaaaagtgg tgctgtgctc accgccgccg attgccgatg
    27301 agatccttta tgcggcgcag ctgtgcggtg tgaaggacgt gtttaacgtc ggcggcgcac
    27361 aggccattgc cgcgctggcg tttggtacgg aatctgtgcc gaaagtggac aaaatcttcg
    27421 ggccgggtaa cgcctttgtc accgaagcga aacgtcaggt gagccagcgt ctggacggtg
    27481 cggctatcga tatgcccgca ggcccgtcgg aagtgttggt cattgccgac agcggcgcaa
    27541 cgccggattt cgtggcttct gatttgcttt ctcaggctga acacggcccg gactcacagg
    27601 tgattttact gacgcctgac gctgatatgg cgcatcaagt tgccgaagcc gtcgaacgcc
    27661 agttagcaga actgccgcgt gccgaaaccg cccgccaggc actgaacgcc agccgcctga
    27721 tcgtgactaa agatttagcg cagtgcgtag agatctccaa ccagtacggc ccggagcacc
    27781 tgatcatcca gacccgcaac gcccgcgatc tggtcgatgg catcaccagc gccggttcgg
    27841 tatttcttgg tgactggtca ccggaatcgg caggtgatta cgcctccggc accaaccacg
    27901 ttctgccgac ttacggttac accgccacct gttccagcct cgggctggca gatttccaga
    27961 agcgtatgac cgtgcaggaa ctgtcgaaag aaggtttctc cgcgctggct tcgaccattg
    28021 aaacattggc cgccgccgag cgcctgaccg cccataaaaa tgccgttact ttgcgtgtta
    28081 acgcccttaa ggagcaagca tgagcaccgt gactattacc gatttagcgc gtgaaaacgt
    28141 ccgcaacctg acgccgtatc agtcggcgcg tcgtctgggc ggtaatggcg acgtctggct
    28201 gaacgccaac gaatacccca ctgccgtgga gtttcagctt actcagcaaa cgctcaaccg
    28261 ctacccggaa tgtcagccga aagcggtgat tgaaaattac gcgcaatatg caggcgtaaa
    28321 accggaacag gtgctggtca gtcgtggcgc ggacgaaggt attgaactgc tgattcgcgc
    28381 tttttgcgaa ccgggtaaag acgccatcct ctactgcccg ccaacgtacg gcatgtacag
    28441 cgtcagcgcc gaaacgattg gcgtcgagtg ccgcacagtg ccgacgcagg acaactggca
    28501 actggactta cagggcattt ccgacaagct ggacggcgta aaagtggtct atgtttgcag
    28561 ccccaacaac ccgacagggc aactgatcaa tccgcaggat tttcgcactc tgctggagct
    28621 aacccgcgga aaagccattg tggttgccga tgaagcctat atcgagtttt gcccgcaggc
    28681 atcgctggct ggctggctga cggaatatcc gcacctggct attttgcgca cactgtcgaa
    28741 agcttttgct ctggcgggcc ttcgttgcgg atttacgctg gcaaacgaag aagtcatcaa
    28801 cctgctgatg aaagtgatcg ccccctaccc gctctcgacg ccggttgccg acattgcggc
    28861 ccaggcgtta agcccgcagg gaatcgtcgc tatgcgcgaa cgagtgacgc aaattattgc
    28921 agaacgcgaa tacctgattg ccgcactgaa agagatcccc tgcgtagagc aggttttcga
    28981 ctccgcaacc aactacattc tggcgcgctt taaagcctcc agcgcagtgt ttaaatcttt
    29041 gtgggatcag ggcattatct tacgtgatca gaataaacaa ccctctttaa gcggctgcct
    29101 gcgaattacc gtcggaaccc gtgaagaaag ccagcgcgtc attgacgcct tacgtgcgga
    29161 gcaagtttga tgagtcagaa gtatcttttt atcgatcgcg atggaaccct gattagcgaa
    29221 ccgccgagtg attttcaggt ggaccgtttt gacaaactcg cctttgaacc aggcgtgatc
    29281 ccggaactgc tgaagctgca aaaagcgggc tacaaactgg taatgatcac caatcaggat
    29341 ggtctgggaa cacaaagttt cccgcaggcg gatttcgatg gcccgcacaa cctgatgatg
    29401 cagatcttca cctcgcaagg cgtacagttt gatgaagtgc tgatttgtcc gcacctgccc
    29461 gccgatgagt gcgactgccg taagccgaaa gtaaaactgg tggaacgtta tctggctgag
    29521 caagcgatgg atcgcgctaa cagttatgtg attggcgatc gcgcgaccga cattcaactg
    29581 gcggaaaaca tgggcattaa tggtttacgc tacgaccgcg aaatcctgaa ctggccaatg
    29641 attggcgagc agctcactaa acgagaccgt tacgctcacg tagtgcgcaa caccaaagag
    29701 acacagattg acgttcaggt gtggctggat cgcgaaggtg gcagcaagat taacaccggt
    29761 gttggcttct ttgatcacat gctggatcag atcgccaccc acggtggttt ccgtatggaa
    29821 atcaacgtca aaggcgacct ctatatcgac gatcaccaca ccgtcgaaga taccggcctg
    29881 gcgctgggcg aagcgttaaa aatggctctt ggcgacaaac gcggtatttg ccgctttggt
    29941 tttgtgctac cgatggacga atgccttgcc cgctgcgcgc tggatatctc tggtcgcccg
    30001 cacctggaat acaaagccga gtttacctac cagcgcgtgg gcgatctcag caccgagatg
    30061 atcgagcact tcttccgttc gctctcatac accatgggcg tgacgctaca cctgaaaacc
    30121 aaaggtaaaa acgatcacca ccgtgtagag agcctgttca aagcctttgg tcgcaccctg
    30181 cgccaagcca tccgcgtgga aggcgacacc ctgccctcgt cgaaaggagt gctgtaatga
    30241 acgtggtgat ccttgatacc ggctgcgcca atctgaactc ggtgaagtca gccattgcgc
    30301 gccacggtta tgaaccaaaa gtcagccgtg acccggacgt ggtgttgctg gccgataaac
    30361 tatttttacc cggcgttggc actgcgcaag cagcgatgga tcaggtgcgt gagcgcgagc
    30421 tgtttgatct catcaaagcc tgtacccaac cggtactggg catctgctta gggatgcaac
    30481 tgctgggtcg gcgcagcgaa gagagcaacg gcgtcgactt gctgggcatc attgacgaag
    30541 acgtgccgaa aatgaccgac tttggtctgc cgctgccgca tatgggctgg aatcgcgttt
    30601 acccgcaggc aggcaaccgc ctgtttcagg ggattgaaga cggcgcgtac ttttactttg
    30661 ttcacagtta cgcaatgccg gtcaatccgt ggaccattgc ccaatgtaat tacggcgaac
    30721 cgttcaccgc ggcggtacaa aaagataact tctacggcgt gcagttccac ccggagcgtt
    30781 ctggtgccgc tggcgctaag ttgctgaaaa acttcctgga gatgtgatga ttattccggc
    30841 attagattta atcgacggca ccgtggtgcg tctccatcag ggcgattacg gcaaacaacg
    30901 cgattacggt aacgacccgc tgccgcgctt acaggattac gccgcgcagg gtgccgaagt
    30961 gctgcatctg gtggatctga ccggggcaaa agatccggca aaacgtcaaa tcccgctgat
    31021 taaaaccctg gtcgcgggcg ttaacgttcc ggtgcaggtt ggcggcggtg tgcgtagcga
    31081 agaagacgtg gcggcgttac tggaagcggg cgttgcgcgc gtggtggttg gctccaccgc
    31141 ggtgaaatca ccagaaaggg tgaaaggctg gtttgaacgt ttcggtgccg atgccttagt
    31201 gctggcgctg gatgtccgta ttgacgagca aggcaacaag caggtggcgg tcagcggctg
    31261 gcaagagaac tcgggcgttt cactggaaca actggtggaa acctatctgc ctgtcggcct
    31321 gaaacatgtg ctgtgtaccg atatctcgcg cgacggcacg ctggcaggct ctaacgtctc
    31381 tttatatgaa gaagtgtgcg ccagataccc gcaggtggca tttcagtcct ccggcggtat
    31441 tggcgacatt aacgatgttg ctgccctgcg aggcactggc gtgcgcggcg taatagttgg
    31501 tcgtgcatta ctggaaggta aattcaccgt gaaggaggcc atcgcatgct ggcaaaacgc
    31561 ataatcccat gtctcgacgt tcgtgatggt caggtggtga aaggcgtaca gtttcgcaac
    31621 catgaaatta ttggcgatat tgtaccgctg gcaaaacgct acgccgaaga aggtgcagac
    31681 gaactggtgt tctacgatat caccgcttcc agcgatggcc gtgtggtaga taaaagctgg
    31741 gtatctcgcg tggcggaagt gatcgacatt ccgttctgcg tggcgggtgg gattaagtct
    31801 ctggaagatg ccgcgaaaat tctttccttt ggcgcggata aaatttccat caactcccct
    31861 gcgctggcag acccaacctt aattactcgc ctggcggatc gctttggcgt gcaatgcatt
    31921 gtggtcggca ttgatacctg gtacgacggc gaaaccggta aatatcatgt gaatcaatat
    31981 accggcgatg aaagccgcac ccgcgtcact cagtgggaaa cactcgactg ggtacaggaa
    32041 gtgcaaaaac gtggagccgg agaaatcgtc ctcaacatga tgaatcagga cggcgtgcgt
    32101 aacggttacg atctcgaaca actgaaaaaa gtgcgtgaag tttgccacgt cccgctaatt
    32161 gcctccggtg gcgcgggcac catggaacac ttcctcgaag ccttccgcga tgccgacgtt
    32221 gacggcgcgc tggctgcttc cgtattccac aaacaaataa tcaatattgg tgaattaaaa
    32281 gcgtacctgg caacacaggg cgtggagatc aggatatgtt aacagaacaa caacgccgcg
    32341 aactggactg ggaaaaaacc gacggactaa tgccggtaat tgtgcaacac gcggtatccg
    32401 gcgaagtgtt aatgctgggc tatatgaacc cggaagcctt agacaaaacc atcgaaagcg
    32461 gcaaagtcac cttcttctcg cgcactaaac agcgactgtg gaccaaaggc gaaacgtcgg
    32521 gcaatttcct caacgtagta aatattgcgc cggactgcga caacgacacg ctactggtac
    32581 tggcgaatcc tatcggacca acctgccaca aaggcaccag cagctgcttc ggcgacaccg
    32641 ctcaccaatg gctgttcctg tatcaactgg aacaactgct cgccgaacgc aaatctgccg
    32701 acccggaaac ctcctatacc gccaaactgt atgccagcgg caccaaacgc attgcgcaga
    32761 aagtgggcga agaaggcgta gaaaccgcac tggcagcgac ggtacatgac cgctttgagc
    32821 tgaccaacga ggcatcggac ttgatgtatc acctgctggt gctgttgcag gatcaggatc
    32881 tggatttaac gacggtaatt gagaacctgc gtaaacggca tcagtgagtt gcagggtaag
    32941 cggatgcgat gttgttgtcg catccggcaa aaaaaaaggg caaggtgtca ccaccctgcc
    33001 cgttttcttt aaagctgcaa agattacttc gcgttataat tacgcagagc gttacgcccc
    33061 agcacaatcc ccgcgccaac catgccgccc agcagcaccg ccagaatcaa ggtaattgcc
    33121 tttttcgggc tatcgcgacg aataggtaac gtcggtttca tcacataacg gtaagcatga
    33181 atatcaagat catcaacctt taagttttca atatccaaca ggttttgacg tgtctgatag
    33241 tagtttggtg agaacaccaa cggacgggtc gcctcatgct taatcatcga ctccagcgct
    33301 tcgctcccca aaaggaacaa cgtatcttgt gtcacatctt cagtctgctg aacctgtggc
    33361 tttgccacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg gatacgcaga
    33421 tctttctgct cctgcgcgac tacttcctgc gttcttaaag agtcctgcaa gtttttccgt
    33481 cccagagcaa tgttgtcttt gagatccttt tctagctctt gattcacttt atcatcaact
    33541 tgctgaatgt attgggccaa cttcatttgt gctccctctg cagtttgccc aacataagaa
    33601 acagtcaatg gtaattgctg gttcttaaca gaaggttcga tggtaagttt ttctggttct
    33661 tcctgattat ccagcgtttc tgctaatgca gagaaggcag aactgaagcg accaattaac
    33721 gtctcctgta aatccgatac tttcggtgca gcctgaccat agataacatt catggcattg
    33781 ttatagccag caatttgccc cacgtcgggc tgagtgataa ttgctgttga cgtccatttc
    33841 tccttcgcta ctgccaaata tccaatagcc agggcaatag ccacaatgac ggaaatgatg
    33901 attgtcatct tgccacgcca caactgcact agtaaatcaa tcaaatcaat ctgttccggg
    33961 tcatggtttt gcccagaaac attattattt tctactctca tcattaccct aactgacgga
    34021 aatttccaag tccgcatagt ttagcgataa tcctagtgaa tgctatagga taagtgataa
    34081 aaataatgaa ttattgggga ggtaacaaaa gacaatcagg gcgtaaatag ccctgataac
    34141 aagatgttag tcgctgccaa agagatcgcg ggtgtatacc ttatctgcca catctttaag
    34201 ctcttctgcc attcggttag agataatgac gtcggcttgt tgtttgaaga tggcgagatc
    34261 acgttccagc cgagagttga agaatgagtc ttctttcatc actggctcgt agatgatcac
    34321 ttcaacgcct ttcgccttga tacgcttcat aatcccctga atagaagacg cacggaagtt
    34381 atctgaaccg ctcttcataa tcagacgata aatacccacc acttgcggtt tgcgtgacaa
    34441 aatggcatcg gcaataaaat ccttacgcgt gcggtttgca tcgacaattg ccgagatcag
    34501 gttattcggc acagactgat agttcgccag taactgcttg gtatctttcg gcaagcaata
    34561 accaccataa ccaaacgacg ggttgttgta atggttgcca atacgcggat cgagacaaac
    34621 gccttcgata atctggcgag tattcagacc taaactttct gcgtagctat ccagttcgtt
    34681 aaagtatgcc acgcgcatcg ctaggtaggt gtttgcaaaa agtttaatcg cttctgcttc
    34741 ggtcgaatcg gtgaacaggg tcgggatatt ttgcttaatc gcgccttcct gtaacaacgc
    34801 agcaaaacgt tctgcgcgtt ctgaacgctc accgatgaca atacgtgaag gatggagatt
    34861 atcgtaaagg gctttacctt cacggagaaa ttccggagag aaaataatat tttcagtacg
    34921 atatttctta tgcatcgctt cggtaaaacc aacgggaacc gttgatttga tgaccataac
    34981 cgcataagga tttatctcaa ctacgtcttt aattactgat tctacactgg atgtattgaa
    35041 ataattagtt ttaggatcat agtcggttgg agtggcgatg atgacataat cagcatcccg
    35101 gtaggcttca tttttatcta atgtggcatt aaagtgtatt ttatctgatt gcaaaaactg
    35161 ctgaatttcc ttatcaacaa taggagatat ccgatcattc agcatagcaa cgcgtgacgg
    35221 taaaatatct aatgccacaa cctcatgatt ttgtgcgatt agaagcccgt ttgacaagcc
    35281 tacatagcca gtaccggaaa tggtgatttt catttcatgc tctcagaatt aacttaactg
    35341 tgaatcatga tgtttttagc atcctgataa gagctaaatg ttttaacgct acgttattag
    35401 ctggtcaacg ctacgaaaaa caattcagat tttctttata agaatagctg atatttatta
    35461 agttaatatt aagcagtgaa atttagtcta taagataaat ggcaaaaaaa aagcccggtg
    35521 caatatacgc cgggcctcaa ttttactgtt ggttaaatca gattaatcca gccattcagt
    35581 atggaacaca ccttctttat caatgcgctt ataagtatgt gcaccgaaat agtcacgctg
    35641 tgcctggatc aggttcgcag gcagaacagc ggcacggtag ctatcgtaat aggcaaccgc
    35701 agcggcgaag gtcggaaccg ggataccgtt ctgtactgca taagcaacga catcacgcag
    35761 cgcctgctgg tagtcatcgg caatttgctt gaagtacgga gccagcagca ggttagcgat
    35821 ctgcggattt tcggcatatg catcggtgat tttctgcagg aactgcgcac ggatgatgca
    35881 gccagcacgg aaaatcttcg cgatttcgcc gtagttcaga tcccagttgt attcttcaga
    35941 cgcagcacgc agctgagaga agccctgagc gtaagaaacg attttgccca gatacagcgc
    36001 acggcgaact ttttcgatga actcagcctt gtcgcctgct gactgagctt gcggaccaga
    36061 gagaacttta gatgcggcaa cgcgctgatc tttcagagaa gagatataac gtgcaaacac
    36121 agactcggta atcagcgaca gcggttctcc gagatccagc gcgctctggc tggtccattt
    36181 accggtacct ttgttagccg cttcatccag gatcacatca accaggtagt taccgtcttc
    36241 atctttttta gtgaagatat ctttggtgat gtcgatcagg tagctgctca gttcaccgtt
    36301 attccactcg gtaaaggtct gtgccagttc ttcgttagag agattcaggc cgcctttcag
    36361 gagagaatag gcttcagcaa tcagttgcat atcaccgtat tcaataccgt tgtgaaccat
    36421 cttcacataa tggcctgcgc catcagcacc gatataggta acgcacggtt cgccatcttc
    36481 agcaacggcg gcgattttgg tcaggatcgg cgcaaccagt tcataggctt ctttctggcc
    36541 accaggcatg atggacggcc ctttcagcgc cccctcttct ccgccggaaa caccggtacc
    36601 gatgaagtta aagccttcgg cagaaagctc acggttacga cgaatggtgt cctggaagaa
    36661 ggtgttacca ccatcgatga tgatgtcgcc tttatcgaga tatggtttga gcgaatcaat
    36721 agcagcatcc gtgcctgcac ctgctttcac cattaacagg atgcgacgag gcgtttccag
    36781 agattcaaca aactctttca ccgtatagta aggaaccagt ttcttgcctg gattttcggc
    36841 aatcacttct tccgtctttt cacgggaacg gttgaaaata gagacggtat aaccacggct
    36901 ttcgatgttg agcgcaaggt tgcgccccat cactgccata ccgactacgc cgatctgttg
    36961 ctttgacatt gtttactcct gtcagggggt ggtcaccgcc gagtgtgcgg cttaaatgtg
    37021 gtatagatgt taactcactc atagagatgg tgaatagcat tacctttgct ataccacact
    37081 gaatatctca ccttttccgt cttttaaaac ttatttatat gaaattactg aattacttgt
    37141 tcagtaactc aagaataagt tttgtctttt cttccatcag cggcacatca ccgcgagatt
    37201 ccacattcaa ccgcaccacc ggttcggtgt tggaggtgcg caggttaaag cgccagtcgg
    37261 caaaggtcat gctgatgcca tcggtgcgat ccaccgccag cgcctcgcgg ctaaaatgct
    37321 gttccacgcg gttaatcgcc tcaacgggtt gcgccagttt gctgttgatc tcaccgcttg
    37381 ccggaaacgc tgccatccgg tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca
    37441 ccagttcggc gaccagcagc cacgggatca tgccgctgtc gcagtaagcg aaatcacgga
    37501 agtaatggtg ggcgctcatt tcaccaccgt agatggcgtc ttccttgcgc atccgttctt
    37561 taataaaggc gtgtccggtt ttcgacatca ccggcgtgcc gcctgcggca gtcaccacat
    37621 caacggtgtt ccaggagaga cgcggatcgt ggatgatctt cgcgccggga tttttttcga
    37681 ggaatgcttc tgccagcagg ccgacaatgt agtagccctc aataaactgc cctttttcgt
    37741 caaacaggaa gcagcggtca aaatcgccat caaaagcaat gcccatatcc gcaccgtgtt
    37801 tgatgaccgc attgcgggtg tcgtcgcggc attccggcag tagcgggtta ggaataccgt
    37861 tggggaaatt gccgtccggc gtgttgtgca ctttgattaa ttccacgggt gcgccaaggg
    37921 ctttaaagcg ggcttcgatg gcgtccacca ccggacccgc tgcgccattc ccggagttaa
    37981 tcaccagctt gagcggcgtg aggtttttga cattgatata accgaacagg tgatcaacgt
    38041 aagcgtcacg cagattgatt tgctgatagc gaccgcgttt ggtttcatca acgggaggaa
    38101 agtcgttggc ttctgccaga cgctggacat cgcgcagtcc ggtatcaccg ctgatcgggc
    38161 gagccccttc gcgcaccagt ttcatgccgt tgtaatccat cgggttatgg ctggcggtaa
    38221 cttcaatgcc gccatccacg ccgagatgga acgtggcgaa atagatctct tcggtgccgg
    38281 acatgccaat atccagcaca tcgacgcccg catcctgtaa acctctcgcc agcgccagtt
    38341 ttaaggtttc gctggtgagg cggacatcac cgcctaacac aatggttttc ggtttgagaa
    38401 attcgccata ggcgcgacca atgcgccagg cgatatcttc attcagttct tcgcctaatt
    38461 tcccgcgaat atcataggct ttaaagcagg ttaatttttt catgtcgtta ccctttttca
    38521 ggcaatagtt ggccctgacc gaaacggcca attttgtttt tgtcgttatt tacatctcgg
    38581 tttcgcattc attgcctgat gcgacgttta cacccgtccg tagcgatccg cgaaacgcac
    38641 cacatcatcc tcttcgagat aagagccgga gcgcacttca attaaatcga gcggaatttt
    38701 ccccgggttt tccaggcaat gcatcgcccc cagcggaata taaatggact cgttttcacc
    38761 aagcagtttg atatcaccat caatggtgac ttttgccgtt cccgcgacaa ccacccagtg
    38821 ttccgcgcgg tgatggtgca tctgtaccga caagccctcg cccggtttca cggtgatgcg
    38881 tttcacctgg tagcggtcgc ccgcgtcgat agagtcatat ttgccccacg ggcgatagac
    38941 ttcgcgatgc acccgatgct catggcgacc atcggctttg atctgctcga ccactttttt
    39001 cacatcctgc accgcgttac ggtcggcaat cagcaccgca tctttggtct gcaccactac
    39061 cagatctttc accccgacgg tggttacgag gccagattcg gcgtacacat agctgttttc
    39121 agttttgtga ttaatcacat cgccgtggca aacgttgccc tcggcggtgt gggcgctaat
    39181 ttcccataat gaggaccagg agccgacatc gctccagccc gcatccatcg gcaccacaac
    39241 ggcatctgcc gtacgttcca tgaccgcgta atccaccgac tcttccgggc aggcgagaaa
    39301 cgcttcttca tccacacgaa taaaatcgag atccggatcg acggcgctca tcgctttttc
    39361 acaggcatcg agaatatccg gacgatattt tttcagttct tcgagatagc gtccggcgcg
    39421 gaacaggaac atgccgctgt tccagtaata ttcgccgctt gccacatagg cctgggcggt
    39481 ttccagattc ggtttttcga caaactgcgc cacttcaaag gccaccgtat cctgctcacc
    39541 cgcagacact tcaccgcgac gaatatagcc ataaccggtt tctggtagat ccggcacaat
    39601 gccgaaggtc accagcttgc ccgcttcggc atatggcatg gcattacgca cggcggcacg
    39661 gaacgcgtct tcatcggcaa tcacatgatc cgccgccaat accagcatta acgggtcgct
    39721 ctccgggcta tgacgttttg ccgccagcgc cgccagcgca atggcaggtg ccgtgtttcg
    39781 ccctgccggt tcgagaataa tgttctcggt aagtttgttc agttgacgca gctgttccgc
    39841 gacaataaag cggtgctgct cattgcaaat caccaccggg ctttcgcact ccacgccgtt
    39901 caggcggcag atggtggttt gcagcatggt gagatcgcct ttcaggcata aaaactgctt
    39961 ggggtaaagt acgcgggaaa gcggccataa gcggctaccg gagccacctg ccatcacaac
    40021 tggatagagt ttcgactgcg ccataattat ccccgaatat catttataaa ttgacgtaac
    40081 acgttctctt tatcgagtgt gcgttcggca tattcacgtg ccaccgtgtt gtgtttgggc
    40141 agcaggagcg cctgacggat ccccgccacc agcgcctcga ccgattccgg ttctacgcaa
    40201 accgcaatgc ccggaaaggt ttcgcaaagc tgccccagtt ctgtgtgggc ctctgcggta
    40261 atcaccgcgt taccgcctac cgccagaata ttggtcagtt tcgacggcaa tacggcatct
    40321 gccgcgccgc gtttttgcac caccagatgg caatcgccca tcttcagcag tgcgggtaaa
    40381 gcgtcatacg attgcagcgg gaaaaattgc atgttgcgca gtccacgctg ctgcgccatt
    40441 ttttccagcc gcgctttgcc gccgccctgc ccgacaatgg caaaaatcag cggttcatcg
    40501 cgcagacgat cggcagcttc aataacgttt tccagcccct gcttttcacc aatattgccg
    40561 gagtaaagaa tgattttttt gttatccggc aggtcaagct ggttacgaag ggcgtcaaca
    40621 tcggcatctg caacatgctg aaaacgggca atttccgacc agttggggaa gaagatgacg
    40681 ttttccgccg ccacgccttt ttcgatggct ttattcatca tcgaacgcga aatcgtggag
    40741 acgttatcga cgttatgcag tccgctacgt tcgaacgccg ttgccagctg tgccactttg
    40801 ctgcctttgc cttttccggc aaggcccagc cccagcatgg cgtccacttc gtaatcctga
    40861 atatgcagca cggtacgcgc accagaaagt ttcgccagca ggcgcattcc cggcgtgcaa
    40921 aacagcgttg gcaccacgcc gataatgcga tccggcttcc agcgacgttg cgccatcaga
    40981 ggaaagaaac tgctgacggc aaaactgccg agatgtaaca ggcgtttcag ggtgctcggc
    41041 tgttttggca cgtacagcgg gcagcgccac accgtggcgg ccccctcttc tcgtttgtac
    41101 cgccaggcgg aatagttctc gcccacctgc cactgcgggt agtaaggcgg tgcggtaata
    41161 acccgcacct catgaccttg tgccgccagc cattccacca tctcgccggt gtatttgccg
    41221 ataccggtta actccggcga gtagttaatg ccgtagacca gtattttcat aatccgggta
    41281 ctccggcacg cttctcagcg agaaaatagg cgcggctgtt agcgtgaaca tcgttactgg
    41341 cgagcagcgc gtccggcgtc agccagcggt aatcgtcatg ctgctcatcc ggcagtatca
    41401 gctcttcttc agcgactctg aagcgaaaac cgagcaccac atagtgagtg gtgaaatccg
    41461 tgccggagaa gttatcgtca taaaagtgct gccagacacc gtaaaactgg cctgctgtta
    41521 tcggcagacg cagccccagt tccgccatcg tcagccgctc gaatgcggct tccagcgttt
    41581 cgtctttctg cacgcgccct cccggcacaa accagtaacc ctgcgccggg cggttggttc
    41641 ttttgccaag cagaaactca ccgcgactgt tctcgacaat aaagtcgaga gagacaagcg
    41701 gggtggagcg cactaccgtg gcaaagtctt cctgacgtaa aaacatcatt acccccgaaa
    41761 gcggtcttga ttctcaagga accactggta agtgctggca agccccgctt ccagtgagat
    41821 ttcgtgatac cagccaagct gatgcaggcg cgtcacatcc agcagtttgc gcggcgtacc
    41881 atccggtttg ctggcatcaa aaaccacccg acctttgtaa cccaccactt tggcgatggt
    41941 ttgcgccagc tcgcggatag tgcagtcaac gcccgtgccg acgttaatgt gcgacagcat
    42001 cggctgggtg ttctccagcc agacttcgtg cgccagctcc atgacatgaa tgctcgccgc
    42061 cgccatatca tcgacgtgca ggaattcacg catcggtgta ccgctgcccc ataccaccac
    42121 gtccggtgca ttctgtgccg tcgcctcgtg gaagcgacgc agcaatgcag ggatcacatg
    42181 cgaattactc gggtggaagt tgtcatgcgg gccatacagg ttggtcggca tgactgagcg
    42241 gtaatcgcgt ccgtactggc ggttgtatga ttcgcacagt ttgatcccgg cgattttggc
    42301 aatggcgtaa ggctcgttgg tcggctccag cgtgccctgc aataactcgc tttctgccat
    42361 cggctgtttt gccagtttcg ggtagataca ggacgatcca agaaacagca gtttgttcac
    42421 gtcgttctga tgcgcggcgt gaatgatgtt gctctcaatc atcatgttct ggtagatgaa
    42481 atccgccgga taggtgttgt tggcaacaat gccgcccact ttcgccgccg ccagatagac
    42541 ctggtcaatg cgttcgctgg caaagaaatc atgcaccgcg cggctgtcca gcaggttcag
    42601 ctcgtcgcgg gtgcgtaata ccagttccac atcaccgcgc tgttcgagct gccgcctgat
    42661 ggcagaaccg accatcccac gatggccagc gataaaaatg cgttgtttac tcatgcttat
    42721 gactccagcg cgatcgccac gtcgtagccg tgagatttca gcagagagtg ttttttcgcc
    42781 gcttcgaggt cattagccac catttcagac accatctctc tgagggtgat ttccggtttc
    42841 cagcccagtt tttcgtgcgc tttggtcggg tcgccgagca gcgtttcaac ttcagccgga
    42901 cggaagtaac gcggatcaac ggcgataatc acatcacccg gtttaacgcc cggcgcgtca
    42961 tgcccggtga cggaaaccac aatgcccttc tcttcaacac ccgtgccttc aaagcgcagt
    43021 ttgatgccca gctgtgccgc tgccatttcc acgaactgac gcacggagta ctgaacgccg
    43081 gtcgcgataa cgaaatcttc cggctgttcc tgttgcagca tcatccactg catttttacg
    43141 tagtctttgg cgtggcccca gtcacgcagg gaatccatat tgccgaggta caggcacgac
    43201 tccagcccct gggcgatgtt ggcgattgcg cgggtgattt tgcgggtaac gaaggtttcg
    43261 ccgcggcgcg gggattcatg gttgaagaga attccgttac aagcgtacat gccgtaggat
    43321 tcgcggtagt taacggtgat ccagtaggcg tacagtttgg cgaccgcata cggagatcgc
    43381 gggtagaacg gcgtggtttc tttctgcgga atttcctgca ccagaccata cagttcagag
    43441 gtggaagcct gatagaaacg agttttcttt tccagaccga ggaagcggat cgcctccagc
    43501 aggcgcagcg tacccatcgc gtcgacgtca gcggtatatt ctggtgactc aaaagagacc
    43561 gcaacgtggc tcattgcgcc caggttgtac acttcatccg gctgtacttc acgcaaaatg
    43621 cgcgtcaggt tagaggtatc actcaggtcg ccataatgca gatggaattt cgggttgcag
    43681 gtgtgcggat cctgataaat gtgatccacg cgctcggtgt tgaatgacga tgcgcgacgc
    43741 ttaataccat gcacctcgta acctttttcc agcagaaact ctgccaggta agaaccgtct
    43801 tgtccggtta caccggtgat gagagcgact tttgacatgt cttattcctc tgtatttttg
    43861 aatttattca gtttcaacgc gttcgcgtat caccactgcg ggattccccc ggcaaaccac
    43921 atttgccgga agcgatttaa aaacactgct tcgtgcaccc acgacggtgc cgtcgccgat
    43981 tgtgacgcct ggggcaacaa agacatcggt tgccagccag catttctcgc caatcacaat
    44041 aggcgtggcg ttaatggtga aatgttgact tgcatggtcg tggctaccgg tgcataaata
    44101 acttttttgc gatatcaccg aatgtgcgcc aatggttatt tcaccgaggg tatataaatt
    44161 gacgtcatcg ccgacccacg cgtaatcacc taaggttaat ttccacggat aggtaatttt
    44221 tactgacgga cgaataacta cgttttttcc tatttttgct ccgaataaac gtaataaaaa
    44281 agcccgccag cgatacaata cttgtggcga ccaggcaaat attgttgcct gtactgccca
    44341 ccataattgc actttaatag cgttgccgcc ccggaaccct ttcggcaccg agaaaccgct
    44401 taaatcttgc attggttttc cttatattca gactttgtta tataaggctt tcgtctttga
    44461 ggtagtacgt tggcgtaaat gccaggataa ttcagcccaa aagccaggca catgtaatat
    44521 ttgtcgttgg acttttttcg cgtcagcaca caattccata ttattggtgg tagatacccc
    44581 acccatggaa aattcagaca ccaggccatt gagtttttta aatgcataac cggctttata
    44641 cattttggct gccagcgcgt agtcggaaga aactttatat tccaggtcat aacgccattt
    44701 tttcaagccg gatacaggga aaaatatcgc ctgatgactg gcgggcaggc tgtgataaat
    44761 ataccagccc ggtttggcgc tacgtttaat tttatgcccg tcgccaaaat ccagcagcgc
    44821 atcgccggtg atcatcatgt tatctttttg catttttaac ttacggacaa aatttgcggc
    44881 atcctgatga aaaatatcgc ccgaattgag aaacaacgcg aacttgcctt gtgccatcgt
    44941 aatgcctttg ttcatggcgt cgtagatacc gttatctggc tcgctgacaa agcgtaggtt
    45001 atagatacca ttgagatttt ccagatactc acgggtgccg tcattggaac cgccatcgac
    45061 aacaatccat tcgaagctga tatcttccgc ctgcgccaga tgcgccagcg aggcatgtgt
    45121 tttgactatc ccttcgaggt tacgaaacgc gacagtgatt atgctaagca acatgttctt
    45181 attcttacct cgtaatattt aacgcttttc gcaaaataaa tggacagacg attaaaaatg
    45241 catattccgg gctaaatatc gaaccggtaa aaaacagcga taccggagta aaaagatata
    45301 attgcacgcg aaaattgcgg ttatcaccaa aagcgttgat catcattttt atcactttcc
    45361 ccatgtacca cagcgataaa aacactgcga accaggaaaa ataaataatc agcagataca
    45421 aaccattgtc tatggttttt ccgacatccg caccgttaaa tattccgaat gatgcgacgt
    45481 attcataaag tgagccaaat ctgactacac cgtcaatatg ggtcaaagaa taaccgacca
    45541 tcaccagcgg accgacaata cgataatacg atgacgatcc ttccgtacct aaatcgccca
    45601 gacgggtgga aatataagga aacgcgatta ccacaccaac caggaataca gccagagaaa
    45661 tcaatgccaa cggtaacttt ttcttaatcg cctctttatt cagatattga aatgcccact
    45721 ccagcaaata aaacaggata aaagtcataa cccctgaaaa cgatccggat aatattatcc
    45781 ctgcgagaat catagcatcg gttttaggcg ttttgatacc aaactgtttg atgctgagcc
    45841 aaattgagat taatgccaga gcgaaaaatg ccggttcgaa ataaagtgct gtcgtgcgct
    45901 tgccgccgaa tttaatgaaa ttcagtacat agctgttgct gtaaatcaga tatttcgaaa
    45961 tcgactccat aatactgctg ccaccggtga ggataatttg cgccatctcc accgccgcca
    46021 gcgccaccac cagcccgatc accagataaa agaaacgtaa tatcttgcga tggttgtgtg
    46081 gcgaaatcgt tttaaagcga atactccaca ccatgccaat aatgatcaca atatagacaa
    46141 acagcatggt tgaagtgacg tatttactgg catccagcga ctgaccaaac agatagttaa
    46201 acgccgtgag tcccgcacca atccctaagg caatcatcaa ttttttaacg ctgatgcgtt
    46261 ctaaaaacag cagtaacaag acaggtaaaa aagtgacgat ggtgatgggg aaactttcgc
    46321 caagctgggc gattttgacg ttaaccagca ggtagatcag cggcagcagc aggtagctac
    46381 agattctgat agaagttgac atactcctcc agcatctgtt gtccactgta ggtggcgcgg
    46441 ctgcgttggc tgaactcagc cagcgtggta ccaaatatcg cctgcgcgat ttccggtttg
    46501 cttaactgca ccagttgcag cacgtcttct tcgtcgacgg ttttaccgcc ggatttttgc
    46561 aacacttccc gtgccgcgtc gctatgggtg gcaatcaccg gcacgccaat tgataacgcc
    46621 tcacacaaaa tcagcgggta gttatcgacg cgagaactga ataccagcgc atccatctga
    46681 ttgagcgcgc tcatcaactt gcgcttgtca gtttcaaagc cgtgattaac cacgttgcca
    46741 gcggtgaacg gcgagaactt accaaaggta tgcagttcga ttttgtcgcc cagcgccatc
    46801 atctcacgca ccagttgctg gttagtcttg ccgtcgtaac gcaagtcatg cgccaccacc
    46861 gcgattttcg gcttgccctg ggtttcgcgc accggaggca agtccgccag aatcgcttcg
    46921 gttgccatat caataccatt attgataatc cggcaacgcc ctggaccgta caggctattg
    46981 aaagcgtcag ccacatgctg gctgggggaa ataaactgac agcccagcgc cagcatctca
    47041 cggaataact ggcgtttgcc cgccaccagc tggtgtgcgc gatcaatctt caccggcgga
    47101 tagttattta aggtcgggca tttctggcag cccgttttcc agccttcgca accgtcggta
    47161 aaggcgcagc gtccggtaac actccagtgg tcgtgcagcg tccagaccag agtgacgtcc
    47221 ggtttatggt ttttcacttt ttcgcaaaag cgcaccacgc tcttaagatt tagccagtag
    47281 ctgtgcagca catgaaaatg caggaccacc ggacccggtg tgcgagtaat ggtgcgatat
    47341 aactcattga aattgccaaa cagatcgcga ttaaacagac gaaacagggc aatattcgcc
    47401 atcgcggtca tccgcggcgt atgtttgatg acctgcggat agttctgatg gctgacgctc
    47461 tctttgccgc ctttgccgta accgtagaca aaatgtgacg ccagcccctg ttgcagcgca
    47521 cgctggtgga gatctaacgc cacacctgct gccccgcctt ccgccagtcg cacattaaat
    47581 tgcagaatat tcatttaatt accttcactc gcgctttttc tcccaccacc agcgcgttgt
    47641 ccgggacaga gtcgagcacc acgctgcccg cacccacggt gacgttgtta ccaagcgtga
    47701 tatcaccaag aataatgacg ttggcaccga gttcgacgcc gttgccaatg tgtggacatg
    47761 ccatgttatc ggcaccacga ttgccgatag tgacgccgtg gcgaatggtg aaatcatccc
    47821 ccgctaccac gtttttattg atcactacgg cgtaaccgtg atggatagta aagcggcggc
    47881 caatggtcgc ggcggcctgg atttcataac cgaaaaagca ttcggtgata atgcgataca
    47941 gcaccagcag cggggccgcc cacagattgt tgaggacgtt ctttttgcgc cacaccgaac
    48001 aaaaatgagc aacacgatag gcaagaacca tgcagcacgg gcgtaaactc cagctgttgg
    48061 cacgcagatc ttccagcatc tttagcgccc ccgaatacca tcagccagac gtttgccgtt
    48121 acgcaccgac agcagcgtca gcaaagtgcg ccaggtcata cgtttattgc ggatctggta
    48181 gagggtaaac agctgatatt ttttgcttgc acggtcaaat ttatctttgt gctttcggta
    48241 aaagtggaag tacccggaga attttttcgg tgacgaggtg atctgcattt caccgtgatt
    48301 gatatgcagg atctgcgtcg cttcttccac tttccacggt tcgccgtact ccaccaccat
    48361 ccgcaggaag atgtcgtaat cctgtgctgc tttcagttcg gtatcgaaca aacactcttt
    48421 gaaacgccat gcccaagtaa agacctgatt gccaatgata ttgcgtttat agaacaggcg
    48481 gcgtgagtac ggcgatttgg gatacagtgg taagcttgcc ggttgggaat aaacttctcc
    48541 ctggcatacg tagtcgttag cgtacaaaaa ggcgtgagtg accagttgct gtttatgggc
    48601 gaggaagacg ctcagacggt tgggtgtcca ttcgtcatcg tcatcgatcc cggtaatgta
    48661 ttccccttgc gccagcataa tcgcctggtt acgtaccgcg cacgccccgc tgttaatgtc
    48721 gttgtgaatg taggtgatac gcggatcgtt gagggcagtg acgtactgtt gcagttgttc
    48781 ccaggaggtg gagcaatcat ccacgatgat catctcccag ttgctgtagt cctggcgcag
    48841 gaccgatttt atcgcccgaa tcgccagttg ttggcggttc caggtcggca tatagattga
    48901 aatcagcgga ttctctttca taggttgttc tcccggctta tgagctggtt tgcttgccgg
    48961 atgcggcgtg agcgccttat ccggcctacg gggcggtgcg aatgcaggcg tcgttatttc
    49021 gcatccgact tatattcgta ttcgtaatag ccataatcct gatacgcgct ggcgcggcgg
    49081 aagatggagt tcagaatcac ccctttcacc ggaataccgt tttgctcaaa gcggctcaga
    49141 ctggtttcca cttctttcaa tgtgttgacc gcatagcgcg ccaccattaa cgtggttcca
    49201 gcgtgacgcc cgacaattgc tgcatcggtc actgccagaa tcggcggcgt atcaatcaac
    49261 accaggtcgt agtttttact cgcccagctc accaattcgg caaagcgttc gctcatcaac
    49321 agttcagaag gatttggcgg cacctgaccg cgcgggatca ggtcaaattt ggcaatagag
    49381 gtcggtttag ctgcggtagt aatatcgccc tggccaatca gaatttccga caggccattg
    49441 acgttattgg tacccaacag ctcgtgggtg tagcctttgc gcatatcgca gtcgatcaac
    49501 aacacccgtt tattggtctg actgatcacc gccgccaggt tggcgcagac aaaggtttta
    49561 ccgattgacg ggctcacccc tgtcatcatc aacacattgt tctgcgcctg catcatcgcg
    49621 aagtgcagac tggtacgcag actgcggatg gcttcaatcg ccagatcggt tggattcccc
    49681 accgccagta gctggctctg tttatagcgt ttaatccctt tgatggtttt gacgctatcg
    49741 cgcgctttct gccattccga cagcgggatg ctggcataga cgctgatacc atgttcttcc
    49801 agcacctgcg ggctttcgat gccgcgatta aacaacgagc gcagcagcac acccacgata
    49861 gagagcatca ggccgaggat aattgcgccg aggataatca gtcctttctt cggtttcagc
    49921 acgccaggct gggtgattgc cgggtcaaca atgcgcacat cgccgacggt gctggcttcg
    49981 gtgattttca gctcctgctc tttattcagc agttgcatat agacctgctg accagactcg
    50041 acatcgcggg tcaggcgcac aatctcctgc tgggttttcg gcatcgccgt tacgcgaccg
    50101 ttaagtttgg ctttctcttc ttccagcgcc tgacgtttct ccagcagcgt gcggtacgcc
    50161 gggtgaactt tggtgtacag cttggagatt tccgcctctt taaaggtcag ttcgttcaac
    50221 tgcgcgtcga tgttcaccat cgaatcgagc accgctttcg cttccagcgg cagatcaaca
    50281 gaatctttat cctgacggaa ggcattcagt ttgttttcgg caacatcgag gcggctacgt
    50341 acttccggta actgttgcgc gaggaaagcg aggcttttcg acgcttccgc cgatttgcgc
    50401 tcaatatttt gttcctgata gttacgggcg atgctgttaa gaatgtcgcg gatctgttcg
    50461 cgatcttcac cggtataagt caggctcagt acgcctgcgt ctttgccgtt ctccgttacc
    50521 gtcaggctgt tttgcagttg gttgatcatc cccaacgtgg agtatttggt gacggtaaac
    50581 tcactgcccg ggcgggcgtg aatggcttca accatcagcg tgacgccttc ttttttcagc
    50641 atctggcccg cttgcccacg ggcgctaaag ccgccatcac tgctcagggt gtagttttta
    50701 ttgtccagca cattaagcgt aaacacctgg cccgccatct ctttcgggcg gttaaaggta
    50761 gtcactttca ccgtctcgtt ctggcgtccc atcagtcgct cccagcccgc cccgaaaatc
    50821 gggaacgtgt ttttgctcac tgcaatatcg aggtcgagat cgtccaccgt tttaccaagc
    50881 accagacgcg aacgaatcaa ctggatctcg gcgtccgatg caggcggttt gttggctaat
    50941 gccgaaccaa tgtcctgcac taacgaattg ccgctgtttt gctcgatttg taccagtgcg
    51001 tcggcactat aaatcggcgt tgcgaagaag gtgtaaacca cggcacagag ggcaaataca
    51061 gcggtgatgc caatcaccca ccagcgcgct tcaatgacgg tgccgaccag gcgaccaata
    51121 tcgatttcat cactgcccgt taccggagcg gcatgttgtt ttactttttc tgtcattctt
    51181 atacctgctc tgcgttcaat gcctgcgccc actggcgggc agaccgttca agtaatgtgt
    51241 acaccgctgc aaacgtttcc cggcttttgc gatacggatc ggggatttca cattcgttat
    51301 cccagtgacc aaacagcatc actttgccgc gcatctcagg tgccatctcg cataagcgtt
    51361 cgatatggcg cttttccatg gtcaaaatca ggtcatagtt gcgacacagg cggcggctga
    51421 tttgtcgtgc acagtggcct tccagagaca gttgatgttc tgcggcgacg ctgatagcgg
    51481 taggatcagc gcccttaccg accagcgcgc cgagtccagc ggactccact ttcagttccg
    51541 ggtgataacg ttgcagtaag cgttccgccg tcggggaacg gcaaatattg ccgacacaga
    51601 caactaatat gttgttaaac atgacgatta ccagttatga atgtcgctgg ctgtatccgt
    51661 catgtaacgg acaccgctaa tagttggcaa caactgattg atcagacggt tccagcggga
    51721 aaccggggcg gtggtgacat acaccacgtc ataaggttgc aggcggaatt ctgtcgccat
    51781 caccagcgac gtggcatcgg acatatccag ctggtagata ttggcaatct tgccattacg
    51841 cccgccctcg cctttcagcg gacgaatgac aaagatgccg ctggcgttgg aggtggtcat
    51901 gtcgatacct tcagcattac ccagggcttc agtcagggtc atgccgctaa agtccatttt
    51961 cagggtgctc tgtttcttca cttcacccat cacaaatact ttcagatcat cattacgcgg
    52021 cacgtagaga atatcgccgg gataaagcag tcggttctga ttgaggtcgc cgttttgcat
    52081 cagcgcctgc aaagaaatgc gttcttcacg accattgtgt gttagcacta cgttgcgcca
    52141 gtcagcggtg tcggtcaggc cacctgcggc gttgatggcg tcgagaatag tcagtggcac
    52201 gttggtgatc gcctgttgac cggatttatt cacctgaccg gagatatagg ccttttgtga
    52261 gcggaaggcg gcgatattaa cgtccacctg cgggtcagcg atgtacgtcg ctaagcgccc
    52321 ggtaatatca ctgcggattt cagcgagcgt tttcccgact acgtgaacct tgccgatata
    52381 cgggtaaaac atagtgccgt caggctgtac ccagttgccg gtgtcgctgg agctgcggta
    52441 ctgaccggct ggcgtggtga gttccgggtg atcccagacg gtgacattca gaacgtcgcc
    52501 cggcccgacg cgatactggt aattcgcgat ctcgctttcc agcgtcatat tggggcgcgc
    52561 tacattcggg cgtgggcgta attggtcaat caggcgcggg gtcagcggat aaacattcac
    52621 cattttgtcg agatcgaaat cagcgtcctg ctgtttgatg acgtctttgc ccatcgtcga
    52681 catattgctg cccggaagta ctgtgcaacc gcttatcaag gttactgaca ccaataatgg
    52741 catcaatttc attttggatt tcatcattgt ttatttatca ctttggcaga gtaattatcc
    52801 tgtgcactat taatagcaat atcgccatgc acatttacct tgcagttaat tgaataaaaa
    52861 tttaactggc atcagtccta aaaaaattga tttcatccgc aggctattga cagaataatt
    52921 cagactggtc tttcaggcat ccagacacgc taccgcccct ggctttttag ccaccaatac
    52981 actgatttag cttaattttt caaaccctct ctgcatacaa tcgttgatga gaaagggtta
    53041 ttgcggaaat taacttccga atataaggtg acattatggt aattgaatat tggctctcca
    53101 ataatgcagg aggaagtgtt acagctaacg gaatagcagg caaggtaaca attcggcaat
    53161 tggctatttt taagaattat attaagtgtc attcaatatg gtttttagga gtttctttag
    53221 gttgacaata tttaatattg cgcctccaca tgcgatattt cttaaataat gttttattat
    53281 taccactttt aattcaggga taatgtgagg ttattaccct caaataatat gagataaata
    53341 gtgctgcaac attgcatttt tgccccgatt tatccatgat cgaattgtga catttgtcat
    53401 acaacgaata ggttttgtac ttactatgga atggattgcc gatccgtcta tctgggccgg
    53461 gttaatcacg ctgattgtga tcgaactggt cctcggcatt gataacctgg tctttattgc
    53521 catcctcgcc gaaaaactac cgccgaggca gcgcgaccgc gcacgggtta ccgggctgct
    53581 gctggcgatg ttaatgcgcc tgttactgct ggcgtcaatc tcctggctgg tcaccctgac
    53641 tcaaccgctg ttcagcttcc gctcgtttac ctttagcgcc cgcgacttaa tcatgctgtt
    53701 tggtggtttc ttcctgttgt tcaaagccac gatggagctg aacgaacggc tggaagggaa
    53761 agacagcaat aatcccacac aacgcaaagg tgcgaagttc tggggcgtgg tgacgcaaat
    53821 tgtggtactg gacgccattt tctcactcga ctcagtgatt accgccgtcg ggatggtcga
    53881 ccatttactg gtcatgatgg ccgccgtggt tatcgctatc agcctgatgt tgatggccag
    53941 caagccgtta acgcaatttg ttaacagtca cccgacgatc gttattctct gcttaagctt
    54001 cctgttgatg attggcttta gcctggtggc agaaggtttc ggcttcgtca ttccgaaagg
    54061 ctacctgtac gctgccattg gtttctcggt gatgatcgag gcgctcaatc agttggctat
    54121 ctttaaccgg cgacgttttc tttccgctaa ccagacgctg cgccagcgga ctaccgaagc
    54181 ggtaatgcgc ctgcttagcg ggcaaaaaga agatgcggaa ctggacgccg aaaccgcgtc
    54241 catgctggtg gatcatggta accagcagat ctttaatccg caggaacggc ggatgattga
    54301 gcgggtactt aatcttaacc agcgtaccgt cagcagcatt atgacgtcgc gccacgatat
    54361 tgagcatatc gatctcaacg cgccggaaga agaaatccgc cagttgctgg agcgaaatca
    54421 gcatacgcgg ctggtggtta ccgacggtga tgacgcagaa gatttgctcg gtgttgttca
    54481 cgttatcgac ctgctacaac agtctctgcg cggcgaaccg ctcaacctgc gggtgttgat
    54541 tcgccagccg ctggtgttcc cggaaacctt gccgttgtta cctgccctgg agcagttccg
    54601 taatgcccgc acgcactttg cttttgtggt ggatgagttt ggctcggtgg aagggattgt
    54661 gacattaagt gacgtcactg aaaccattgt cggtaactta ccgaacgaag tggaagagat
    54721 cgacgcccgc catgatattc agaagaatgc ggacggttcc tggacggcga atggtcatat
    54781 gccgctggaa gatctggtgc aatatgtgcc gctgccgctg gatgaaaaac gtgaatatca
    54841 caccattgcc gggctgttga tggaatattt acagcgtatt ccaaaacctg gcgaagaagt
    54901 tcaggtgggg gattatttgc ttaaaacgtt gcaggtggaa agccatcgcg tgcagaaggt
    54961 gcagattatt ccgctgcata aggatggcga gttggagtac gaggtgtgat ggtatcggtc
    55021 attttaccca atagccgaaa ggaataagat gagggaaccg cgccagcggc gatttaccgc
    55081 cgggagcccg ggttgccagg gtggtggcga ttgagccacc ctggcacgtt caccggcttg
    55141 agcgttccat agtagcgagg aacatcaagt gaacggaaca gccttcgatc ttacatattc
    55201 agaatataat gatgccgcca cgaacgcctg atgcgacgct ttacgcgtct tatcaggcct
    55261 acaggactgg tcacctggta acctacatct tctccagcaa cttcttcaca tccttgccat
    55321 tgcgggaatc tttattccgc tcggcccagt cattcaggcg acgtttcgct tcgtcctgta
    55381 gatgtttgcg cagtaactga tccacttgca ggctgtaatt gagttgctgc cagttgccat
    55441 aaacccgcag cggtactggc gtttctttca ggaaatcaat cagtttactt tccccgttcc
    55501 agccacccac gacccgaata tcaaactggg tatcgcaggt ttgatctgcc agattcaaca
    55561 tgccttcccc tgtcagcgcc agcacaggcg attgaccttg catgtcgttt aacgtcacga
    55621 cgccatcctt caacgtcaaa tcggtggtaa agcggtcaag ccgcgtcacg ttatcaaagt
    55681 tttcagcggc cttcacatca ccgccattac gctctaccgc ttgctgaatc atctgctgga
    55741 agttcatccc ttccatgcgc gtgtcggtca tttcgacatg tgcctgtcct tgccagttgt
    55801 ggcggaatgc gtcggcatct atgtcagcac cggagaaatc accagccagt gacatttttc
    55861 cggtcaacga aatcggatag ttaaacgcct tcagaatggt accaatctca acgttttcca
    55921 gccgtggctg gaagtttatc cgcggattta ttgatgtcgc gtccagcgtg cccggcagtg
    55981 aaacctgtcc accgttaagt ttgccctgca gttgagtaat ttccagcaaa cccgacttgt
    56041 tggtcatttg cgtggcaaca tctgtaaaat tcattccgcg ccagcgcacg ttactggcct
    56101 gcaacaaaat atcagccgta aagccttgca gtccctgata ggccggttca tcaatacgcg
    56161 aagaaatgac cgggcgcggc aacgttgatt ggctctgccc ctgctgcgcg gcaccgtttt
    56221 caccattcgc ggtttcatta agcgggatga ggttgtcaag attcagttgc gggaattgca
    56281 gtctcagctg ccattccggt ttctctgtca gcgtgacctg tgcttgcccg ctcagcgtac
    56341 tgtcattggc ggtcaagctg atttggttaa atgaaaggcg tttatgtgac tcctgccact
    56401 gggcctggaa actcccctgc ccctgaatac cttgttttgg taaatcggca ccctgcaact
    56461 gccagttaat ttgttcaata gccgccgtta aatcatgcgg ataatcagaa gcatccaccg
    56521 taccgttaag ggatattgtg agatcgcgct gatcgcgatt aacccgcccg gagaactcaa
    56581 atgagccacg atgttgggga tcttgttcca tttgcaggcg gatattgcgg attgtcacct
    56641 gctcgtcatc ttcatgctgg aacaccagca cgctgtccgc caccttaaga ctggatatat
    56701 caaacgacca tccgcgatcg tctgacagat ccggcaaggt attgtcgcgc ggtgcaaccg
    56761 gagcgtcttc actgcgcacc gcttccgtct gcggcgtcag ttggatcact gcccctttta
    56821 gcatcacctg cttaacgctc agttgatgac tcagtagtgg taaaagcgcc acgtccagac
    56881 gcatgttgtc ggcgcgaacc agtggctggc ttgcgccctg ggcggtgaga gacattcgcc
    56941 cggagaggat actaagctgc ggccagacgt gccaacgcag tggcccgtcg agctgcaatt
    57001 gataaccgct acgtgcagca acttgcttga ccatatagtc gcggaaatca ttcggattca
    57061 ccagcaacac taacgcagat aacccggcca ccagcacgac caggagtatc atcagcgtcg
    57121 tcagaaatcg tctcatggta tcctcaatgg gcctgaatta gtctttatcg attcggctgg
    57181 ctaccgcgcc ctgctggttg cgatatttcg catcttcacg gcggttgtaa ggtcgcgccg
    57241 ccgggccgga aagcggctca aagctcagcg caccaattaa catgcccgga cgcagcgcca
    57301 gcggcagctt accggagttg tagaactcca gcacaatgca accagaccag cccggatcga
    57361 tacggtgcgc ggtgacgtgc accatcagcc ccagacgcgc caaagaggaa cgcccgtcca
    57421 gccagcccac cagatcggct ggcagcgtca ccgactccag cgtcaccgcc agcgccagct
    57481 ctcctgggtg aagatagaac gcctcgccct cgtcgagaac gatctcatcg ctcatcacgc
    57541 ggtcaagcgc ggcgctcact tcatctttgg gaccgctcag atcgataaac gctgccgtgt
    57601 gaccacggaa ggtacgaaat ttattgccca ggcgtacatc caccgtcgcg ccgttaatac
    57661 gctccactgg cggacgtggg ttgatcgaca aacggccttc atcaagccag gcttcaatat
    57721 ctcggtcaca cagacgcatg gcattttctc ctttcgcgca tcactccctt aacgccaatt
    57781 acgtcaaggg cataccaggt tatcactgaa cggtacacaa tttatcaagc ttattcaaag
    57841 aactgactta ttttcgcttt caatatatcg atcgcgatgc ggtttttccc gccgcgcggc
    57901 acgataatgt ccgcatattg tttagaaggc tcaatgaatt gcaggaacat cgggcgcacg
    57961 gttttttgat attgcgccat cacggaatcc attgaacgcc cacgctcgtt aacgtcacgc
    58021 ttgatgcggc gcatcaggca gatatccagc ggggtatcaa cgaaaatgga gaagttaagt
    58081 tcgtcacgca aacgcgcatc cgtcagcaac aaaatgcctt cgagaatgat gaccttcttc
    58141 ggctcaaccg tcaccgtttc tttcatacgc gtatgttcaa catagctgta aaccggcagg
    58201 tcaattgccg agccgcgttt caacgcttgt aaatgctcaa gcagcaggct gtgatccatc
    58261 gcgctgggat ggtcgtagtt ggtcttaacg cgttcttcca tcgacagatg gctttgatct
    58321 ttgtaatagc agtcttcggg aattacgccg atgtgttcat caccgacttg ctcacgtaat
    58381 tcacgataaa gggtactggc aataagactc ttgccggaag ccgatgcgcc agcgataccg
    58441 ataatgacgc actgatgaga ctgatcagtc atatatttag cgacctgatt aacctggatg
    58501 ttaggaaggg ggcgacgaag cgccaaacgc ggcaattata gggatttcat ccgtctgata
    58561 ccagtcgaat agcgttgccg cgcgctcaga gttaattgtt gacaaagaat tcccggtggc
    58621 aaattacgtt gatcagtttt atgtaaggta aaaaatgtta tacgcagttg cgcaaattat
    58681 ccgcctttac gtcactttat gagcaattcg catataaaat gtaaaacttt tgtactagca
    58741 taaacacaga aacgaatact ggcgacctgg tcttgcggat aaagcggtaa tgagcaaaca
    58801 atcacagcat gtattaattg ccctgcccca cccgctgctt cacctggtca gtttaggttt
    58861 agtctcgttt atctttaccc ttttctcgct tgagctttcg cagtttggca cccaactcgc
    58921 cccactgtgg ttcccgacgt ccatcatgat ggtggcgttt tatcgccatg ccgggcgcat
    58981 gtggccggga attgcgctga gctgttcgct gggaaatatc gccgcatcca tcctgctttt
    59041 ttccaccagc tcgctgaaca tgacctggac gaccatcaat attgttgaag ccgtggtcgg
    59101 ggcagtgctg ctacgtaaat tgctgccgtg gtataacccc ttgcaaaatc tggctgactg
    59161 gctgcgtctg gcactcggca gcgccattgt tccgcctctg ttggggggtg ttctggttgt
    59221 cctgctgacg cccggagacg atcctctcag ggcatttttg atatgggtac tgtcagaatc
    59281 catcggcgct ctggcactgg tgccgctggg attgttattt aaaccacact atctgctgcg
    59341 ccatcgcaac ccacggttgc tttttgagtc gctgctcacg ttagccatca cactgacgtt
    59401 aagctggctt tcgatgctgt atctgccgtg gccttttact ttcattattg tgctgttgat
    59461 gtggagcgcc gtgcgcctgc cacgaatgga agcctttttg atcttcctta ccacggtgat
    59521 gatggtgtcg ctgatgatgg ccgcggatcc ctccctgctt gctacgccgc gtacgtacct
    59581 gatgagccat atgccgtggc taccgttttt gctgatcctg ctgcccgcca acatcatgac
    59641 catggtgatg tatgcctttc gtgcggaacg caaacacatt tccgaaagcg aaacccgctt
    59701 tcggaacgcg atggaatatt ccgctatcgg tatggcgtta gtgggcaccg agggacaatg
    59761 gctgcaaacc aacaaagcgc tctgccagtt tctcgggtac agtcaggaag agctgcgcgg
    59821 actcaccttt cagcaactga cctggccgga ggatctcaat aaagatctcc aacaggttga
    59881 aaagctgata agcggtgaaa taaacaccta ttcaatggaa aaacgctact acaaccgcaa
    59941 tggcgatgtt gtctgggcgt tgcttgccgt ctcactggtg cgccacacgg atggcacgcc
    60001 gctctatttt atcgctcaga ttgaagacat taacgagcta aaacgcaccg aacaggtcaa
    60061 tcagcaactg atggagcgca tcacgctggc taacgaagcg ggcgggattg gcatctggga
    60121 gtgggagttg aagccgaatg tttttagctg ggataagcgg atgttcgagc tgtatgaaat
    60181 tcctccacat atcaaaccga actggcaggt gtggtacgag tgcgtgctgc cggaagatcg
    60241 ccagcatgcc gaaaaagtga ttcgtgattc gttgcaatca cgctcgccct ttaaactgga
    60301 atttcgcatt accgtaaaag acggtattcg ccatatccgc gccctcgcca accgggtact
    60361 gaataaagaa ggcgaagtcg aacgtctcct cggcatcaat atggatatga ccgaagtgaa
    60421 acagcttaac gaggcattgt ttcaggaaaa agagcgcctg cacattacgc ttgattccat
    60481 cggtgaagcc gtggtctgta ttgatatggc gatgaaaatt acctttatga atccagtggc
    60541 ggagaagatg agcggctgga cgcaggaaga agcgttaggt gttccgctcc tgacggtgtt
    60601 gcatattact tttggcgaca acggaccatt aatggagaac atttacagtg ccgacacctc
    60661 acgttccgca atcgaacaag atgtggtgtt gcactgtcgg agcggcggca gttacgacgt
    60721 gcattacagt attacgccgt taagtactct ggacggcagc aatattggtt cggttctggt
    60781 gattcaggac gtcaccgaat cacgcaaaat gctgcgccag ctgagctaca gcgcctccca
    60841 tgatgcactg acgcatctcg ccaaccgcgc cagttttgag aaacaactgc gtatcctgct
    60901 gcaaacggta aacagtacgc atcagcgaca tgccctggtg tttatcgatc ttgatcgctt
    60961 taaagcggtg aatgacagcg ccgggcatgc ggcgggcgac gctttgctgc gcgaactggc
    61021 gtcgttgatg ctgagtatgc tgcgctccag tgacgtgctg gcgcggctcg gcggtgatga
    61081 atttggtctg ctgctgccag actgcaatgt tgaaagcgct cgttttatcg ctacacgtat
    61141 tatcagtgct gtgaatgact atcactttat atgggaaggc cgggtacatc gggtaggtgc
    61201 cagcgccggg attaccttga tcgatgacaa caatcatcag gcggcagaag tgatgtcgca
    61261 ggctgatatc gcctgttatg cctccaaaaa tggtggacgg ggccgggtga cggtttacga
    61321 accacagcaa gctgccgcac atagcgaacg ggcggcgatg tcgcttgatg aacagtggcg
    61381 gatgattaaa gagaatcagt tgatgatgat cgcccacggt gtcgcttcgc cgcggatccc
    61441 ggaagcgcgt aatttgtggc tgatttcgct taagctctgg agttgcgaag gcgaaattat
    61501 tgatgaacaa acatttcgtc gtagcttcag cgatccggca cttagccatg ctcttgaccg
    61561 acgggtattc cacgaatttt tccagcaggc cgccaaagcg gttgccagta aaggcataag
    61621 catcgccctc cccctttccg ttgccggttt gagtagcgcc acgctggtga atgatctgct
    61681 tgagcagctg gaaaatagcc ctctaccacc acggttatta catctgatta ttccggctga
    61741 agcgatttta gatcacgcag aaagcgtgca aaaactgcag ctggcgggat gtcggatagt
    61801 gctcagccag gtgggccgcg atctgcaaat cttcaactcg ctgaaagcga atatggcaga
    61861 ttacctgcta cttgatggtg agttatgcgc caacgtgcag ggtaatttga tggatgagat
    61921 gctgattacg attattcagg ggcacgctca gcgactcggg atgaaaacca tcgccgggcc
    61981 agtcgtttta cccttagtga tggatacgct ttctggcatc ggcgtcgatc tgatttatgg
    62041 tgatgtgatt gccgatgccc aaccgctgga tttgctggtg aatagcagtt atttcgcgat
    62101 taactgagag agcgtttgcc cattgcctga tgcgacgcta acgcgtctta tcatgcctac
    62161 aaatcgctca ttccccagac cggataaggc gctcgcaccg catccggcga ccaacgtcat
    62221 gcttcgtctg gttgccagcc ttccgtatac cagatatgca acagcgcata agaacgccag
    62281 ggcttccagc gctcggcata acggcggatt tgtgccggtg tcattcccgg aaatcgccgt
    62341 ttaatcagat aatcatccgg cagaaaaaca tctttcgcct gccagccacg caaagcaaaa
    62401 taattcgccg tccagcgccc gatacccgga aaagtttgca gcgttttcat cgcctgctcc
    62461 acatcgcccg gaattgtcat tggtaagctg ccctccagcg ccgcatttgc cagatgaatc
    62521 agtgcctctg cccgtttcaa cggcatgcct aacgctttta atgcctgcgg gtcggctgct
    62581 gccagccgct gaggcgtggg gaagcagata tactccggaa aatcatccag ccgttcgcca
    62641 taaagctgta ccactttggc agtcaatttt gccgccatcg ccacgctcac cagttggcct
    62701 aaaatcgccc gcacaccctg ctcaaaagca tcaatacagc cgggtaaacg caatccgggc
    62761 cgccccgcgc ctaactttcc caacgcaccg ttaacaatct gcgggttaca ttgcagatca
    62821 agcaggcggc tcattttcgc cagacactct gcggcaacag gttctaaacc tgcacttaaa
    62881 tttatgtgca gagtatggcg ggctatatcc ggaatagcag tcactacgcc gcgatattcg
    62941 cccaccgcca gactacgggc ataataattg tccgcgaccg tttccacacc gctcaccgca
    63001 cgggcggcga gaaatcccaa catccacgac cagtcatacg gcggctgcca gttcagggta
    63061 tacatcgcat ctccttgtta atccgctttc agcataaacg ttattcagac gttacgcttt
    63121 gctttcatat tccggttgtc gcgacagcaa catttcgcta aagtcacgcc ccttcttcac
    63181 cggcatgggg attaattcgt gtttattggt tttgattacg gtacagcaaa ctgttcagtg
    63241 gcggtcatgc gtgacggtaa accgcaatta ctgaaaatgg aaaacgacag cacgctgctg
    63301 ccttcaatgc tttgcgcgcc aacgcgtgaa tcggtaagcg aatggctgta ccgccatcat
    63361 gatgttccgg cagacgacga tgaaacgcag gcgctgttac gtcgggcgat tcgttataac
    63421 cgcgaagaag atatcgatgt tacggcgaaa agcgtgcagt tcggtctttc ctcactggca
    63481 cagtacattg atgatccaga agaagtgtgg tttgtgaaat caccaaaatc gttcctcggt
    63541 gccagcggct taaaaccgca gcaggtagcg ctgtttgagg atctggtctg cgcaatgatg
    63601 ttgcacattc gccagcaggc gcaggcgcag ctgccagaag cgattactca ggcggtgatt
    63661 ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag caaacgccca ggcgcaagag
    63721 attctggaac gcgcggcgaa gcgtgccgga ttcagggacg tggtattcca gtacgagccg
    63781 atcgcggctg ggctggatta cgaagccacc ttgcaggaag aaaaacgggt gctggtggtg
    63841 gatatcggcg gtggtacgac tgactgttca ttgctgctga tggggccgca gtggcgttcg
    63901 cgtctcgatc gtgaagccag tctgctgggt cacagtggtt gtcgtattgg cggtaacgat
    63961 ctggatatcg ccctggcgtt taaaaacctg atgccactgc tgggtatggg tggcgaaacc
    64021 gaaaaaggca tcgccctgcc gatcctgccg tggtggaatg cggttgccat caacgacgta
    64081 cctgcgcaga gtgatttcta cagtagtgcc aacggtcgtc tgcttaacga tctggtacgc
    64141 gatgcccgcg aaccggaaaa agtggccctg ttacagaaag tctggcgtca gcgtttaagc
    64201 tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc tttcaagcgt agcggaaacc
    64261 cgcgcctcac tgccgtttat cagcaatgaa ctggctacgc tgattagcca gcgagggctg
    64321 gaaagcgccc tcagccagcc actggcgcgg attctggaac aggtgcaact ggccctggat
    64381 aacgcccagg aaaaaccgga cgttatctac ctgaccggcg gtagcgcccg ttctccgcag
    64441 attaaaaaag cgctggcaga acagttgccg ggcattccga ttgcaggcgg cgatgacttt
    64501 ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg tgtttcgtta attgaaaaat
    64561 ggctcagcag cggcttaaat ctgcctgcgc ctgagccagt ttttctgctg cctgacgtaa
    64621 cggttccatc aacggcatgg tccgtgctgg agcatgaagg cggaatcgct gcaactctgt
    64681 cgcgccgact gtcaacgccg attccagccg atgtcgtttg gcggtcattt tcgccattac
    64741 cgcttgtctg tctgctggcg ttatcgcttc attcggcctg aacacaaaac ggcgttcaca
    64801 gttcgctttc cagtcgataa ccgcctgggt cagatggtcg ccaaaccctt tcacttgctt
    64861 aacgccacga cgggtaacat ctgcggctgt ttcaatacca aaagaccgta acgccgcttt
    64921 acgcgcaggg ccaacgccgg gaatagaggc aacatcaata aaaaatccct ccagaaactt
    64981 ctgcttctgc cgttcccttg cagtgtcatg aagtgctgcc agagcgcgtt tctcttcttc
    65041 cggtaacccg agaatttcgt ccttcatttt ttcgagcatc gtccgtttgg cgataaaacc
    65101 ttccaggccg cctacctgct ggatctggcg gactaaatgg tcatagtcca ttttcgcacg
    65161 attaaatgcc tcacggcgtt gctgaacttc tgctttgtac gctttgcttg tcaaactgcc
    65221 aataatccag atagccgcca gcacaggaac caaaataata taacgcggtt ctgcctgaag
    65281 gccgcaaagc agcagcgata atgctgacag tgcgatctca agcagaatga tgtattcgcg
    65341 ccgtaacagg cctaaaggaa gcggcctgcc agtcggttgg aaatgatcgg gtaatggcag
    65401 ttgcaatgct ggcggtgcta ctgacgccat caccatcgcc cagacttttg ccagcacaaa
    65461 atccccgccg gtggtaatga cctcttcgcc gagatcaata aaatagataa caccttgatt
    65521 atccagcgca caccatgggc agtcagccaa atgaccggga taaacatgca ttgccgaaac
    65581 ggtacatttc tttaactgtt ggcgtagaga atccagtgct gatacccacg ctttagccgt
    65641 cggacgcccg gttgccacgc cactttccgt aaacgcctgc tgaaacatgg cttcaacatc
    65701 actcggtaac atcgatagcg gaatcgatcg tggcggcggt tttaaaccac gtcgctgatt
    65761 atctgacgcg taggcataac ggaaatgggt aatatccgtc tccagcgcat tacccgcatc
    65821 agagataagc ggcacaccag aataaggatg ccggccacca aacaagacgt gaaaaatcag
    65881 caacgcaagg ccaaaattat cgtgattttc tgtgcgttca aagccgacaa atgacgacaa
    65941 ggtttgtagc tctggcggcg taaaatgcga cacgccgact tcgcataaat gcagtgtgcc
    66001 attggcgtta atctgaaagg agtcgctatc aatcaacacc actttgctgt cgcgacctac
    66061 cataaaactg ttctggttta cgtcacccac gacgtgcccg tgctcgtgaa ccgtagcaaa
    66121 agatgaagca atattgcgcg caacatagag tagaaaatcc cacgcacaat gagggtaacg
    66181 ttgacgacga tgtgccgggc tatagatcat atgaatcggt tctttaccag aaacttttgg
    66241 catcataaaa ccgataactt ttccgcctcg tccaccgtga agcgttgcct gcggccaggc
    66301 gacataattc aacaattgcg cgtcagctgt cgcagccata aaggcaagtt tgtcctgttt
    66361 taaggcgggt ggcggcgtgt gataaatctt ggcgacgcta tcgacaaact cattgatatc
    66421 ataaaccgcg ccttcaccgc ctttgcccag ttcacggcct ggcgtaacgc actcaccagt
    66481 agcagtatat aaagtgggtt tcatggtaac cacagcgcca gtgccaacgt tttatcatca
    66541 tccgtacgct cgtttaccgc cgggctggac aaaaactgct ttaacaattc aggtaataaa
    66601 tcgagttgtt cctgcgttgc tgacgccagt ccattgaaaa acggggtaaa gaaaggcaca
    66661 tgaggagaat tatccagcat atttaacgcc agccgctgaa taccatccgt aaatgctgca
    66721 actttatgcg cacgcccagt gctggtgaaa gtttccagtc tggaaacggc atcttcgtcg
    66781 gtaatgaagt gcgtcatgtt ggcatattca cccaccatcg gcgtgagagg caattgcaga
    66841 ccgtgaccca aatctaccac cacgccgcca tcgccaatct gcataatcaa ggtgccgtca
    66901 ggcgatgaaa tcaatcccag aaacgtgcag gcaaaatcgc gaactgctaa tgctttggct
    66961 tctgcctcgg caaaaagtcg ttgacgaata gtcagtacca tatttgtggc gaggacatca
    67021 ttaagcccca attcaccgtc ctgtactttt tgcaccataa acgccatcgc ttcattgacg
    67081 gcgagcgtcg cgccttctcc tccctgcgaa acgctacctg cgccatcagc aaggaacatc
    67141 accagtaatg gttgctggtc gtttaaccag acaacctgca tctggcaggc atcctgacaa
    67201 ggcaaatcag cactaatgtg cgatgttcca acagcggatg cataaaccag acgccagctc
    67261 acactgacgt ccagcctttt ggcgcttcca gcacaacttc cgttcccggc gtggagcggg
    67321 aaaccgaacg cagcgaactg gataaccagc taaacagttc acggaattgt aatccctgca
    67381 gtggcaaagg ctgacgaacg ctgatttgtg ccagagtctt catatccgcc ccctgaacac
    67441 cgatggaaaa gaaggcaaac ttcttatctt cttccccctg aaacactttg ttggcggcag
    67501 cctgccactc atcggttgga gcgccatcgg tgatcaggaa aatccacggg cgataatacg
    67561 aaataccatt tgcgcggtac tcacgtttcc tctcttcaac catatccagg gctttggtaa
    67621 tggcggcccc cataggcgta tccccctggg caaacaggat gggcgggaaa aagttagcgg
    67681 cgctggtaaa gggttgttcc acatgcaccg ggccgaacgt cacaatgccg agttccactc
    67741 tttttaaagc cagcggatca gcaagcagtt catcacgaaa ggtaaccaat ccggcgttaa
    67801 gttcattaat aggtctgccg ctcatcgagc cagaaacatc cagcagcaaa atgcaggggc
    67861 aacgtggttc cgggttactg gcaaaatcgc tggtggcaaa cgtgatttgt tcgctcataa
    67921 gaaatccttt cttagagaat tatcgaggtc cggtatcata ccgattgcaa taatgaaata
    67981 agagcaaacg aagaaacttt cagaattagg cccgtcggcg cagaaaattt tgctattcgc
    68041 ctcgcaagaa cggagagccg tcgattgaca ctaagggcgg agtgacataa tttcaggagt
    68101 gagggttagg gagaggtttc cccctccccc tggtgttctt agtaagcctg gaagctaatc
    68161 actaagagta tcaccagtat gatgacgtgc ttcatcataa ccctttcctt attaaaagcc
    68221 ctcttctccg ggagagggtt tcccgtttca gcgtcccgct gaaatcgtcg gcttacctcc
    68281 tttcgccctg aatgcagtct atcgctaacg cgtgaacaaa atagtttcat ctgtgattat
    68341 tgcgtaacga ctcgcaaaga atatttgctg gcaggatcgc agactacaaa gcctgcggat
    68401 tgacaatctt atcgtgaagg cataatttca ggagtgaggg tagagcgggg tttcccccgc
    68461 cctggtagtc ttagtaagcg gggaagctta tgactaagag caccacgatg atgagtagct
    68521 tcatcatgac cctttcctta ttaaggcccc ttcctcggga ggggctttcc cgtttcagcg
    68581 tcccgctgaa atcgtcggct tacctccttt cgccctgtat gcagtctatc gctgacttgc
    68641 ggataaaata gtttccagtg tgattattgc gtaacgtctc gcaaagaata tttgctggca
    68701 ggatcgcaga ctgcaaagcc tgcagattga caatcttatc gtgaaggcat actttcagga
    68761 gtgagggtag agcggggttt cccccgccct ggtagcctta gtaagctggc cagctaatga
    68821 ctaagagcac cacgatgatg agtagcttca tcatgaccct ttccttattt atggcccctt
    68881 cctcgggagg ggctttcccg tttcagcgtc ccgctgaaat cggcggctta cctcctttcg
    68941 ccatgcaagc agtctatcgc taacgcgtag ataaaatagt ttcctgtgtt attactggat
    69001 gcgtgctcgc aaatgtgccc gtcattcaga cgattcccga cagtgtttca taattcctcc
    69061 atttttctcc cttattggct ggctacacta gtatcattcc gcgaaacgtt tcaggaagag
    69121 aaactcttaa cgatgaaagg cagttataaa tcccgttggg taatcgtggt ggttatcgcc
    69181 gccatcgccg cattctggtt ctggcaaggc cgcaatgact cccagagtgc agccccaggg
    69241 gcgacgaaac aagcgcagca atcgccagcg ggtggtcgcc gtggtatgcg ttccggccca
    69301 ttagccccgg ttcaggcggc gaccgccgta gaacaggcag ttccgcgtta cctcaccggg
    69361 cttggcacca ttatcgccgc taatactgtt acggtgcgca gccgcgtgga cggtcaactg
    69421 atggcgttac atttccagga aggccagcag gtcaaagcag gcgatttact ggcagaaatt
    69481 gaccccagcc agttcaaagt tgcattagca caaacccagg gccaactggc aaaagataaa
    69541 gccacgcttg ctaacgcccg ccgtgatctg gcgcgttatc agcaactggc aaaaaccaat
    69601 ctcgtctccc gtcaggagct ggatgcccaa caggcgctgg tcagtgaaac cgaaggcacc
    69661 attaaggctg atgaagcaag cgtcgccagc gcgcagttgc aactcgactg gagccgcatc
    69721 accgcaccag tcgatggtcg cgttggtctc aagcaggttg atgttggtaa ccaaatctcc
    69781 agtggtgata ccaccggaat tgtggtgatc acccagacgc atcctatcga tttagtcttt
    69841 accctgccgg aaagcgatat cgctactgta gtgcaggcgc aaaaagccgg aaaaccgctg
    69901 gtggtagaag cctgggatcg caccaattcg aagaaattaa gtgaaggcac gctgttaagc
    69961 ctcgataacc aaatcgatgc cacaaccggt acgattaaag tgaaagcacg ctttaataat
    70021 caggatgatg cgctgtttcc caatcagttt gttaacgcgc gcatgttagt cgacaccgaa
    70081 caaaacgccg tagtgatccc aacagccgcc ctgcaaatgg gcaatgaagg ccattttgtc
    70141 tgggtgctga atagcgaaaa caaggtcagc aaacatctgg tgacgccggg cattcaggac
    70201 agtcagaaag tggtgatccg tgcaggtatt tctgcgggcg atcgcgtggt gacagacggc
    70261 attgatcgcc tgaccgaagg ggcgaaagtg gaagtggtgg aagcccagag cgccaccact
    70321 ccggaagaga aagccaccag ccgcgaatac gcgaaaaaag gagcacgctc ctgatgcagg
    70381 tgttaccccc gagcagcaca ggcggcccgt cgcgcctgtt tattatgcgt cctgtcgcca
    70441 ccacgctgct gatggtggcg atcttactcg ccgggattat cggttatcgc gccctgcccg
    70501 tttcggcgct gccggaagtg gactatccga ccattcaggt ggtcacgctc tacccaggtg
    70561 ccagcccgga tgtcatgacc tctgccgtta ccgcgccgct cgaacgccag ttcgggcaga
    70621 tgtctggcct gaaacagatg tcgtcgcaaa gttccggcgg tgcgtcagtt atcactttgc
    70681 agttccagct aacattaccg ctcgatgtcg ccgagcagga agtgcaggcc gcgattaacg
    70741 ctgcgaccaa cttgttgccg agcgatctgc ctaacccgcc ggtttacagc aaagtgaacc
    70801 cggcagatcc gccgatcatg acgctcgccg tcacctcaac cgccatgccg atgacgcaag
    70861 tggaagatat ggtggaaacc cgcgtcgcgc agaaaatctc gcagatttcc ggcgtcggcc
    70921 tggtgacgct ttccggcggt cagcgtccgg ctgttcgcgt caaacttaac gctcaggcga
    70981 ttgccgccct cggcctgacc agcgaaaccg tgcgcaccgc cattaccggc gctaacgtta
    71041 actcggcaaa aggtagcctc gacggccctt cccgtgcagt cacgctttcc gcgaacgacc
    71101 agatgcaatc cgccgaagag tatcgccagc taatcatcgc ctaccagaac ggcgcgccaa
    71161 ttcgtctggg cgatgtcgca actgtagagc aaggtgcaga aaacagctgg ctcggcgcgt
    71221 gggcgaacaa agaacaggcc attgtgatga atgttcagcg ccagcccggt gctaacatta
    71281 tctccaccgc cgacagcatt cggcagatgc tgccacagct cactgagagt ctgccgaaat
    71341 cggtgaaggt gacagtgctt tccgatcgca ccaccaatat ccgcgcatcc gtcgatgata
    71401 ctcagtttga attgatgatg gctatcgcgc tggtagtcat gattatctac ctgtttttgc
    71461 gcaatattcc ggcgaccatc attcccggtg ttgctgtacc gctgtcgtta atcggcactt
    71521 tcgcggttat ggtgtttctc gatttttcaa tcaataacct gacactgatg gcgttaacta
    71581 tcgccaccgg attcgtggtc gatgacgcca tcgtggtgat cgaaaacatt tcccgctata
    71641 tcgaaaaagg cgaaaaaccg ttggcggcgg cgctcaaggg cgcaggtgaa atcggcttta
    71701 ccattatctc gctgaccttc tcactgattg cggtgttgat cccactgctg tttatgggcg
    71761 atatcgtcgg gcgactgttc cgcgaatttg ctattaccct ggcggtagcg attttgatct
    71821 cagcggtggt gtcgctgacc ctgacaccga tgatgtgcgc gcggatgctc agccaggagt
    71881 cgttgcgtaa acagaaccgc ttctcccgtg cctcggaaaa aatgttcgac aggataatcg
    71941 ccgcctatgg tcgtggactg gcgaaagtgc tgaatcatcc gtggctgacc ttaagcgtgg
    72001 cactcagcac gctgctgctt agcgtgctgc tgtgggtgtt cattccgaaa ggtttcttcc
    72061 cggtacagga caatggcatt attcagggca ctttgcaggc accgcaatcc agctccttta
    72121 ccaatatggc ccagcgacaa cgccaggtcg cggacgtgat tttgcaggat ccggcagtgc
    72181 aaagcctgac ctcatttgtt ggcgttgatg gcactaaccc gtcgctgaat agtgcacgtt
    72241 tacaaatcaa cctcaaaccg ttggatgaac gtgatgatcg ggtgcaaaaa gtcatcgccc
    72301 gtctgcaaac ggcggtagat aaagtgccgg gcgtcgatct cttcctgcaa ccaacgcagg
    72361 atctgactat tgatactcag gtcagccgca cccagtacca gtttaccttg caggccacct
    72421 cactggatgc gctcagtacc tgggtgccac agttgatgga aaaactccag caactgccac
    72481 agctttctga tgtctccagc gactggcagg accaagggct ggtggcgtat gtcaatgttg
    72541 atcgcgacag cgccagccgt ctggggatca gcatggcgga tgtcgataac gccctgtaca
    72601 acgcgtttgg tcagcgactg atttccacta tttatactca ggctaaccag tatcgcgtag
    72661 tgctggagca caacaccgaa attaccccgg gcctcgcggc gctggatacc attcgcctga
    72721 ccagtagcga cggcggcgtg gtgccgttaa gctcaattgc caaagttgaa cagcgttttg
    72781 cgccgctttc catcaaccat ctggatcagt tcccggtaac gaccatctcc tttaacgtgc
    72841 cggataacta ttcgctgggc gatgcggtgc aggagattat ggacaccgaa aagacgctga
    72901 atctgccggt ggatatcacc acgcaattcc agggcagcac cctcgccttc cagtcggcgc
    72961 tgggcagcac tgtctggctg attgtcgcgg cggtggtggc gatgtatatc gtgctcggca
    73021 ttctgtacga gagctttatt cacccgatca ccattctctc gacgctaccc accgcagggg
    73081 tcggcgcgct gctggcatta atgattgccg gtagcgaact ggatgtgatt gcgattatcg
    73141 gcattatttt gctgatcggt attgtgaaga agaacgccat catgatgatc gacttcgcgc
    73201 tggctgctga gcgcgagcaa ggcatgtcgc cgcgcgatgc aatctaccag gcttgtctgt
    73261 tgcgttttcg tccgatcctg atgaccaccc tggcggctct gcttggcgcg ctgccgctga
    73321 tgttgagtac cggggtcggc gcggaactgc gtcgtccgtt aggtatcggc atggtcggcg
    73381 gtctgattgt cagccaggtg ctgacgctgt tcaccacgcc ggtgatttat ctgctgttcg
    73441 accgcctggc attgtggacc aaaagtcgct ttgctcgcca tgaagaggag gcgtaagtga
    73501 agttttttgc cctcttcatt taccgcccgg tggcgacgat tttactgtcg gtcgccatta
    73561 ccctgtgcgg catactgggc ttccgtatgc tgccggtcgc cccgctgccg caggtcgatt
    73621 ttccggtgat tatggtcagc gcctcgctgc ccggtgcgtc accagaaaca atggcgtctt
    73681 ccgttgccac gccgctggag cgctcgcttg ggcgcattgc cggggtcagc gaaatgacct
    73741 ccagcagttc gctcggcagc acacgtatta ttttgcagtt tgattttgac cgggacatca
    73801 acggcgcagc gcgtgatgtg caggcggcga tcaacgctgc acaaagtttg ctgcccagtg
    73861 ggatgcccag ccgtccgacc tatcgcaaag cgaacccgtc ggatgcgcca attatgatcc
    73921 tcacgctgac atccgatact tattcgcagg gtgaactgta cgatttcgcc tcgacgcagc
    73981 tggctccgac gatttcgcaa atcgacggtg ttggtgatgt cgatgtcgga ggcagctcac
    74041 tgcccgccgt acgcgtcggg ctgaatccgc aggcgctgtt taatcagggc gtgtcgctgg
    74101 acgacgtacg caccgccatc agcaatgcca acgtgcgtaa accgcagggc gcgctggaag
    74161 atggcactca ccgctggcag atccagacca atgatgagct aaaaaccgcc gctgaatatc
    74221 agccgttgat tattcactac aacaacggcg gcgcggttcg tctgggcgat gtggcgacgg
    74281 tgaccgactc agtgcaggat gtgcgcaacg ccgggatgac caacgccaaa ccggctattt
    74341 tactgatgat ccgcaaactg ccggaagcca atattatcca gacggttgac agcatccggg
    74401 caaaattacc ggagttgcag gaaaccattc cggcggcgat tgatctgcaa attgcccagg
    74461 atcgctcccc caccattcgc gcctcgctgg aagaagtcga gcaaacgctg attatctcgg
    74521 tggcgctggt gattctggtg gtgtttttat tcctgcgctc gggtcgcgcc actattattc
    74581 ccgccgtttc ggtgccggtt tcgctgattg gtacgtttgc ggcgatgtac ctgtgcggat
    74641 tcagtctcaa taacctttcg ttaatggcgc tcaccatcgc tactggtttc gtggtggatg
    74701 acgccatcgt ggtgctggaa aacattgcac gtcatctgga agcgggaatg aaaccgttgc
    74761 aagccgcact gcaaggtact cgcgaagtcg gttttacggt gctgtcgatg agtctgtcac
    74821 tggtggcggt gttcctgccg ctgctgttga tgggcggatt gccgggccga ctgttacgcg
    74881 agtttgccgt gacgctttct gtcgccattg gtatttcgtt gctggtttct ctgacattaa
    74941 cgccaatgat gtgtggctgg atgctgaaag ccagcaagcc gcgcgagcaa aagcgactgc
    75001 gtggttttgg tcgcatgttg gtagccctgc aacaaggcta cggcaagtca ctgaaatggg
    75061 tgctcaatca tacccgtctg gtgggcgtgg tgctgcttgg caccattgcg ctgaatatct
    75121 ggctgtatat ctcgatcccg aaaaccttct tcccggagca ggacactggc gtgttgatgg
    75181 gcgggattca ggcggatcag agtatttcgt ttcaggcgat gcgcggtaag ttgcaggatt
    75241 tcatgaaaat tatccgtgac gatccggcag tggataatgt caccggcttt acaggcggtt
    75301 cgcgagtgaa cagcgggatg atgtttatca ccctcaagcc acgcgacgaa cgcagcgaaa
    75361 cggcgcagca aattatcgac cgtctgcgcg taaaactggc gaaagaaccg ggggcgaatc
    75421 tgttcctgat ggcggtacag gatattcgcg ttggtgggcg tcagtcgaac gccagctacc
    75481 agtacacgtt gttatccgac gacctggcgg cactgcgcga atgggagccg aaaatccgca
    75541 aaaaactggc gacgttgccg gaactggcgg acgtgaactc cgatcagcag gataacggcg
    75601 cggagatgaa tctggtttac gaccgcgaca ccatggcacg gctgggaatc gacgtgcagg
    75661 ccgccaacag cctgttaaat aacgccttcg gtcagcggca aatctcgacc atttaccagc
    75721 cgatgaacca gtataaagtg gtgatggaag tggatccgcg ctatacccag gacatcagtg
    75781 cgctggaaaa aatgttcgtt atcaataacg aaggcaaagc gatcccgctg tcgtatttcg
    75841 ctaaatggca accggcgaac gctccgctgt cggtgaatca tcagggatta tcggcggcct
    75901 cgaccatctc gttcaacctg ccgaccggaa aatcgctctc ggacgccagt gcggcgatcg
    75961 atcgcgcaat gacccagctt ggtgtgcctt cgacggtgcg cggcagtttt gccggaacag
    76021 cgcaggtgtt ccaggagacg atgaactcac aggtgatcct gattatcgcc gccatcgcca
    76081 cggtgtatat cgtgctgggt atcctttacg agagttacgt acacccactg acgattctct
    76141 ccaccctgcc ctcggcgggc gttggtgcgc tgctggcgct ggagctgttc aatgccccgt
    76201 tcagcctaat agccctgata gggatcatgc tattaatcgg catcgtaaag aaaaacgcca
    76261 ttatgatggt cgattttgcg ctcgaagccc aacggcacgg taacctgacg ccgcaggaag
    76321 ctattttcca ggcctgtctg ctgcgttttc gcccgattat gatgactacc ctggcggcgc
    76381 tgtttggcgc gctgccgctg gtgttgtcgg gcggtgacgg ctcggagctg cggcaccccc
    76441 tggggatcac cattgtcggt ggactggtaa tgagccagct tctgacgctg tataccacgc
    76501 cggtggtgta tctctttttc gaccgtctgc ggctgcgttt ttcgcgtaaa cctaaacaaa
    76561 cggtaaccga gtaaatgaca gaacttcccg acagcacccg ctggcaattg tggattgtgg
    76621 ctttcggctt ctttatgcag tcgctggaca ccaccatcgt taacaccgcc cttccctcaa
    76681 tggcgcaaag cctcggggaa agtccgttgc atatgcacat ggtcattgtc tcttatgtgc
    76741 tgaccgtggc ggtgatgctg cccgccagcg gctggctggc ggacaaagtc ggcgtgcgca
    76801 atattttctt taccgccatc gtgctgttta ctctcggttc actgttttgc gcgctttccg
    76861 gcacgctgaa cgaactgttg ctggcacgcg cgttacaggg cgttggcggt gcgatgatgg
    76921 tgccggtcgg caggttgacg gtgatgaaaa tcgtaccgcg cgagcaatat atggcggcga
    76981 tgacctttgt cacgttaccc ggtcaggtcg gtccgctgct cggtccggcg ctcggcggtc
    77041 tgctggtgga gtacgcgtcg tggcactgga tctttttgat caacattccg gtggggatta
    77101 tcggtgcgat cgccacattg ctgttaatgc cgaactacac catgcagacg cggcgctttg
    77161 atctttccgg ctttttattg ctggcggttg gcatggctgt attaaccctg gcgctggacg
    77221 gcagtaaagg tacaggttta tcgccgctgg cgattgctgg cctggtcgca gttggcgtgg
    77281 tggcactggt gctttatctg ctgcacgcca gaaataacaa ccgcgccctg ttcagtctga
    77341 aactgttccg cactcgtacc ttttcgctgg gcctggcggg gagctttgcc ggacgtattg
    77401 gcagtggcat gttgcccttt atgacaccgg ttttcctgca aattggcctc ggtttctcgc
    77461 cgtttcatgc cggactgatg atgatcccga tggtacttgg cagcatggga atgaagcgaa
    77521 ttgtggttca ggtggtgaat cgctttggtt atcgtcgggt actggtagcg accacgctgg
    77581 gtctgtcgct gatcaccctg ttgtttatga ctaccgctct gttgggctgg tactacgttt
    77641 tgccgttcgt cctgttttta caagggatgg tcaactcgac ccgtttctcc tccatgaaca
    77701 ccctgacgct gaaagatctc ccggacaatc tggcgagcag cggcaacagt ctgctgtcga
    77761 tgattatgca attgtcgatg agtatcggcg tcaccatcgc cgggctgttg ctgggacttt
    77821 ttggttcgca gcatgtcagc gtcgacagcg gcaccacaca aaccgtcttt atgtacacct
    77881 ggcttagcat ggcgtttatc atcgcccttc cggcgttcat ctttgccaga gtgccgaacg
    77941 atacgcatca aaatgtagct atttcgcggc gaaaaaggag cgcgcaatga agttctggcg
    78001 acctggtatt accggcaaac tgtttctggc gattttcgcc acctgcattg ttttgctgat
    78061 cagtatgcac tgggcggtgc gtatcagttt tgagcgtggc tttattgatt acatcaagca
    78121 tggtaatgaa cagcgattac aactgttaag tgatgcgctt ggcgagcagt atgcgcagca
    78181 tggcaactgg cgcttcctgc gcaacaatga tcgctttgtc tttcagatcc tgcgttcatt
    78241 tgaacacgat aattcggaag ataaacccgg cccgggtatg ccaccgcacg gctggcgtac
    78301 ccagttctgg gtggttgatc aaaacaacaa agtgctggtt ggtccgcgag cgccgattcc
    78361 acctgacggt acacggcgac ccattctggt caacggtgcg gaagttggcg cggtgatcgc
    78421 ctcccccgtt gagcggttaa cgcgcaatac tgatatcaat ttcgataaac aacagcggca
    78481 aaccagctgg ttgattgtcg ccctggcaac gttactcgcg gcacttgcca cttttctgct
    78541 ggcgcgcggt ttactggcac cggtaaaacg acttgtcgat ggcacgcaca aactggcggc
    78601 gggcgatttc actacccgcg taacgcccac cagtgaagat gaactgggca aactggcgca
    78661 agacttcaac cagcttgcca gcacactgga gaaaaaccag caaatgcggc gcgattttat
    78721 ggccgatatt tctcacgaac tgcgtacgcc attagcggtg ctgcgcggtg aactggaagc
    78781 tattcaggat ggcgtgcgca aattcacgcc ggagacggtg gcgtctttac aggcggaggt
    78841 cggtacactg accaaactgg ttgacgatct ccatcagttg tcgatgtctg atgaaggcgc
    78901 cctcgcctat caaaaagcac cggtagattt gatcccactg ctggaagtgg cgggcggcgc
    78961 atttcgcgaa cgattcgcca gccgtggcct gaaactgcaa ttttccctgc cagacagtat
    79021 taccgtattt ggcgatcgcg accgtttaat gcagttattc aataatttac ttgaaaacag
    79081 cctgcgctac actgacagcg gcggcagcct gaaaatctct gccgagcagc acgacaaaac
    79141 ggtgcgcctg acctttgccg acagtgcgcc aggtgtcagt gacgagcagc tacaaaaatt
    79201 atttgaacgt ttttatcgca ccgaaggttc ccgcaaccgc gccagcggtg gttccgggct
    79261 ggggctggcg atttgcctga acattgttga agcacataat ggccgcatta ttgctgccca
    79321 ttcgcctttt ggcggggtaa gcattacagt agagttaccg ctggaacggg atttacagag
    79381 agaagtatga ccgagttacc aatcgacgaa aacacaccgc gtattttgat cgtggaagat
    79441 gaaccgaagc tggggcagtt gctcattgat tatctgcgtg ctgcgagcta tgcgccgacg
    79501 cttatcagcc acggcgatca ggtactgccg tatgtgcgcc agacaccacc ggatctgatc
    79561 ctgttggatc tgatgctccc tggcaccgat ggcttgacgc tgtgccggga aattcgtcgt
    79621 ttttctgaca ttcctatcgt gatggtgacg gcaaaaatcg aagagatcga ccgcctgctc
    79681 ggactggaga ttggcgcaga tgactacatc tgcaagccgt acagcccacg ggaagtggta
    79741 gcgcgcgtca aaaccatttt gcgccgttgc aaaccgcagc gcgaattgca gcaacaggat
    79801 gccgaaagcc cgttgattat cgacgaaggt cgttttcagg cttcatggcg cggtaaaatg
    79861 cttgacctga cgcctgcgga atttcgtctg ttgaaaacgc tctctcatga accaggaaaa
    79921 gtgttctccc gcgagcaatt gctcaatcat ctttatgacg actaccgcgt agtaaccgac
    79981 cgcaccattg acagccacat taaaaacctg cgccgcaagc tggaatctct cgacgccgaa
    80041 cagtcattta tccgcgccgt ttacggcgtt ggttaccgct gggaagccga cgcctgccgc
    80101 atcgtttagt tttagcgaca tgactttgtt agccggagat ggcgttcaga ccagatccgg
    80161 caacattatc ccacgcatgg tcagcaaact ga
//
LOCUS       DAZPEP010000002        74589 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZPEP010000002 DAZPEP010000000
VERSION     DAZPEP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.002"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1015
                     /locus_tag="ACHUT9_000080"
     CDS             <1..1015
                     /locus_tag="ACHUT9_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1346665.1"
                     /translation="KISSDGCLKKEQMVVSAIASTPQLPMLGEKRLVSSVTKEDLLFV
                     RRDLLTGYQKLSNGKISSIKGRSVVTVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPL
                     KKSKIEPDPLTRDEFIRFIEACRHQQTKNLWIIAVYTGIRHGELVSLAWEDIDLKART
                     ITIRRNYTKLGEFTPPKTDAGTGRTIHLVQPAIDALKSQAEMTMLGKQHSVEVKQREY
                     GRSTVHKCTFVFSPQVIKQRQFSGPHYKVDSIRESWTSILKRAGLRHRKSYQSRHTYA
                     CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELLNKRLSESVPCMPHK
                     KVG"
     gene            1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUT9_000081"
     CDS             1051..1737
                     /gene="mlrA"
                     /locus_tag="ACHUT9_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFR1346666.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1797..1904
                     /locus_tag="ACHUT9_000082"
     CDS             1797..1904
                     /locus_tag="ACHUT9_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFR1346667.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUT9_000083"
     CDS             complement(1885..2616)
                     /gene="yehW"
                     /locus_tag="ACHUT9_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFR1346668.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUT9_000084"
     CDS             complement(2621..3547)
                     /gene="yehX"
                     /locus_tag="ACHUT9_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFR1346669.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUT9_000085"
     CDS             complement(3540..4697)
                     /gene="yehY"
                     /locus_tag="ACHUT9_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFR1346670.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUT9_000086"
     CDS             complement(4704..5621)
                     /gene="osmF"
                     /locus_tag="ACHUT9_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFR1346671.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUT9_000087"
     CDS             complement(5873..8170)
                     /gene="bglX"
                     /locus_tag="ACHUT9_000087"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFR1346672.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8366..10081
                     /gene="dld"
                     /locus_tag="ACHUT9_000088"
     CDS             8366..10081
                     /gene="dld"
                     /locus_tag="ACHUT9_000088"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1346673.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUT9_000089"
     CDS             complement(10120..11052)
                     /gene="pbpG"
                     /locus_tag="ACHUT9_000089"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFR1346674.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11226..11813)
                     /locus_tag="ACHUT9_000090"
     CDS             complement(11226..11813)
                     /locus_tag="ACHUT9_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFR1346675.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11983..12561
                     /locus_tag="ACHUT9_000091"
     CDS             11983..12561
                     /locus_tag="ACHUT9_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1346676.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12691..13452)
                     /locus_tag="ACHUT9_000092"
     CDS             complement(12691..13452)
                     /locus_tag="ACHUT9_000092"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1346677.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUT9_000093"
     CDS             complement(13505..14941)
                     /gene="mdtQ"
                     /locus_tag="ACHUT9_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFR1346678.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15165..15248
                     /gene="yohP"
                     /locus_tag="ACHUT9_000094"
     CDS             15165..15248
                     /gene="yohP"
                     /locus_tag="ACHUT9_000094"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFR1346679.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="ACHUT9_000095"
     CDS             complement(15621..16568)
                     /gene="dusC"
                     /locus_tag="ACHUT9_000095"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFR1346680.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKA
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16807..17205
                     /locus_tag="ACHUT9_000096"
     CDS             16807..17205
                     /locus_tag="ACHUT9_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFR1346681.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17202..17897
                     /locus_tag="ACHUT9_000097"
     CDS             17202..17897
                     /locus_tag="ACHUT9_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFR1346682.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            18027..18911
                     /gene="cdd"
                     /locus_tag="ACHUT9_000098"
     CDS             18027..18911
                     /gene="cdd"
                     /locus_tag="ACHUT9_000098"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFR1346683.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            19061..19780
                     /gene="sanA"
                     /locus_tag="ACHUT9_000099"
     CDS             19061..19780
                     /gene="sanA"
                     /locus_tag="ACHUT9_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFR1346684.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19783..20022
                     /locus_tag="ACHUT9_000100"
     CDS             19783..20022
                     /locus_tag="ACHUT9_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFR1346685.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20216..21454
                     /gene="preT"
                     /locus_tag="ACHUT9_000101"
                     /pseudo
     CDS             20216..21454
                     /gene="preT"
                     /locus_tag="ACHUT9_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21448..22683
                     /gene="preA"
                     /locus_tag="ACHUT9_000102"
     CDS             21448..22683
                     /gene="preA"
                     /locus_tag="ACHUT9_000102"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFR1346686.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="ACHUT9_000103"
     CDS             complement(22810..23820)
                     /gene="mglC"
                     /locus_tag="ACHUT9_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFR1346687.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="ACHUT9_000104"
     CDS             complement(23836..25356)
                     /gene="mglA"
                     /locus_tag="ACHUT9_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFR1346688.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKFRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="ACHUT9_000105"
     CDS             complement(25417..26415)
                     /gene="mglB"
                     /locus_tag="ACHUT9_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFR1346689.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26695..27693)
                     /gene="galS"
                     /locus_tag="ACHUT9_000106"
     CDS             complement(26695..27693)
                     /gene="galS"
                     /locus_tag="ACHUT9_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFR1346690.1"
                     /translation="MATVSRVLNNSTLVSADTREAVMKAVSELDYRPNANAQALATQV
                     SDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAEKERHAIEVLIRQRC
                     NALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDNLSGARMATRMLLNN
                     GHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGTPDMPGGEAAMVELL
                     GRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIPIARYTDPQLTTVRY
                     PIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQNAAAITNSTNQAM"
     gene            complement(27877..29034)
                     /gene="yeiB"
                     /locus_tag="ACHUT9_000107"
     CDS             complement(27877..29034)
                     /gene="yeiB"
                     /locus_tag="ACHUT9_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFR1346691.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(29051..29719)
                     /gene="folE"
                     /locus_tag="ACHUT9_000108"
     CDS             complement(29051..29719)
                     /gene="folE"
                     /locus_tag="ACHUT9_000108"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFR1346692.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29977..30813
                     /gene="yeiG"
                     /locus_tag="ACHUT9_000109"
     CDS             29977..30813
                     /gene="yeiG"
                     /locus_tag="ACHUT9_000109"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1346693.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="ACHUT9_000110"
     CDS             complement(30845..32836)
                     /gene="cirA"
                     /locus_tag="ACHUT9_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFR1346694.1"
                     /translation="MFRLNPFVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="ACHUT9_000111"
     CDS             complement(33128..34597)
                     /gene="lysP"
                     /locus_tag="ACHUT9_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFR1346695.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34802..35683)
                     /gene="yieE"
                     /locus_tag="ACHUT9_000112"
     CDS             complement(34802..35683)
                     /gene="yieE"
                     /locus_tag="ACHUT9_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFR1346696.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35782..36831
                     /locus_tag="ACHUT9_000113"
     CDS             35782..36831
                     /locus_tag="ACHUT9_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFR1346697.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36905..37762
                     /gene="nfo"
                     /locus_tag="ACHUT9_000114"
     CDS             36905..37762
                     /gene="nfo"
                     /locus_tag="ACHUT9_000114"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFR1346698.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37766..38854
                     /locus_tag="ACHUT9_000115"
     CDS             37766..38854
                     /locus_tag="ACHUT9_000115"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFR1346699.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38910..40160)
                     /locus_tag="ACHUT9_000116"
     CDS             complement(38910..40160)
                     /locus_tag="ACHUT9_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1346700.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="ACHUT9_000117"
     CDS             complement(40260..41201)
                     /gene="rihB"
                     /locus_tag="ACHUT9_000117"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFR1346701.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41331..42029
                     /locus_tag="ACHUT9_000118"
     CDS             41331..42029
                     /locus_tag="ACHUT9_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFR1346702.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42096..43346)
                     /locus_tag="ACHUT9_000119"
     CDS             complement(42096..43346)
                     /locus_tag="ACHUT9_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFR1346703.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="ACHUT9_000120"
     CDS             complement(43440..44378)
                     /gene="psuG"
                     /locus_tag="ACHUT9_000120"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFR1346704.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44366..45307)
                     /locus_tag="ACHUT9_000121"
     CDS             complement(44366..45307)
                     /locus_tag="ACHUT9_000121"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFR1346705.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="ACHUT9_000122"
     CDS             complement(45765..47456)
                     /gene="fruA"
                     /locus_tag="ACHUT9_000122"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFR1346706.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="ACHUT9_000123"
     CDS             complement(47473..48411)
                     /gene="fruK"
                     /locus_tag="ACHUT9_000123"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFR1346707.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="ACHUT9_000124"
     CDS             complement(48411..49541)
                     /gene="fruB"
                     /locus_tag="ACHUT9_000124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFR1346708.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49909..51090
                     /gene="setB"
                     /locus_tag="ACHUT9_000125"
     CDS             49909..51090
                     /gene="setB"
                     /locus_tag="ACHUT9_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFR1346709.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51087..51341)
                     /locus_tag="ACHUT9_000126"
     CDS             complement(51087..51341)
                     /locus_tag="ACHUT9_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFR1346710.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51496..52068
                     /gene="yeiP"
                     /locus_tag="ACHUT9_000127"
     CDS             51496..52068
                     /gene="yeiP"
                     /locus_tag="ACHUT9_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFR1346711.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52291..53757
                     /locus_tag="ACHUT9_000128"
     CDS             52291..53757
                     /locus_tag="ACHUT9_000128"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1346712.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53875..54861
                     /gene="yeiR"
                     /locus_tag="ACHUT9_000129"
     CDS             53875..54861
                     /gene="yeiR"
                     /locus_tag="ACHUT9_000129"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFR1346713.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54900..55613
                     /gene="lpxT"
                     /locus_tag="ACHUT9_000130"
     CDS             54900..55613
                     /gene="lpxT"
                     /locus_tag="ACHUT9_000130"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFR1346714.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            56025..56591
                     /gene="mepS"
                     /locus_tag="ACHUT9_000131"
     CDS             56025..56591
                     /gene="mepS"
                     /locus_tag="ACHUT9_000131"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFR1346715.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56772..58328
                     /locus_tag="ACHUT9_000132"
     CDS             56772..58328
                     /locus_tag="ACHUT9_000132"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFR1346716.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58410..60224
                     /locus_tag="ACHUT9_000133"
     CDS             58410..60224
                     /locus_tag="ACHUT9_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFR1346717.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60225..61319
                     /locus_tag="ACHUT9_000134"
     CDS             60225..61319
                     /locus_tag="ACHUT9_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFR1346718.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61319..62344
                     /locus_tag="ACHUT9_000135"
     CDS             61319..62344
                     /locus_tag="ACHUT9_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1346719.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62346..63935
                     /gene="yejF"
                     /locus_tag="ACHUT9_000136"
     CDS             62346..63935
                     /gene="yejF"
                     /locus_tag="ACHUT9_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFR1346720.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVHALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63939..64283)
                     /locus_tag="ACHUT9_000137"
     CDS             complement(63939..64283)
                     /locus_tag="ACHUT9_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFR1346721.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64616..65673)
                     /locus_tag="ACHUT9_000138"
                     /pseudo
     CDS             complement(64616..65673)
                     /locus_tag="ACHUT9_000138"
                     /inference="COORDINATES: protein motif:HMM:NF008314.0"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="ACHUT9_000139"
     CDS             complement(65764..66459)
                     /gene="rsuA"
                     /locus_tag="ACHUT9_000139"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFR1346722.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66609..68369
                     /locus_tag="ACHUT9_000140"
     CDS             66609..68369
                     /locus_tag="ACHUT9_000140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFR1346723.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68494..68778
                     /gene="rplY"
                     /locus_tag="ACHUT9_000141"
     CDS             68494..68778
                     /gene="rplY"
                     /locus_tag="ACHUT9_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFR1346724.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="ACHUT9_000142"
     CDS             complement(68917..69924)
                     /gene="yejK"
                     /locus_tag="ACHUT9_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFR1346725.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70106..70333
                     /locus_tag="ACHUT9_000143"
     CDS             70106..70333
                     /locus_tag="ACHUT9_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFR1346726.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70353..72113
                     /gene="yejM"
                     /locus_tag="ACHUT9_000144"
     CDS             70353..72113
                     /gene="yejM"
                     /locus_tag="ACHUT9_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFR1346727.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72188..72264
                     /locus_tag="ACHUT9_000145"
     tRNA            72188..72264
                     /locus_tag="ACHUT9_000145"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72222..72224,aa:Pro,seq:ggg)
     gene            complement(72367..74589)
                     /gene="yejO"
                     /locus_tag="ACHUT9_000146"
                     /pseudo
     CDS             complement(72367..74589)
                     /gene="yejO"
                     /locus_tag="ACHUT9_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18316 a        19661 c        18301 g        18311 t
ORIGIN      
        1 aaaaatatca tcagatggct gcttaaaaaa agaacaaatg gtagtgtccg ctattgccag
       61 tacacctcag ttgccaatgt taggtgaaaa aagactggtt tcatctgtaa caaaagagga
      121 tttgcttttc gtaaggagag atctgttgac cggttatcaa aagctttcta acggaaaaat
      181 ttcttccata aaaggacgct cagtggtcac agtaaactac tatatgacaa ccatagctgg
      241 aatgtttcaa tttgcaacag ataatggtta tacatcagga aatccattta acggtctgac
      301 accattaaaa aagtccaaga tagaaccaga tcccctcacc cgtgacgaat ttattcgttt
      361 tattgaggct tgccgtcatc aacaaacaaa aaacctgtgg attatcgctg tatacacggg
      421 tattcgtcac ggggagctgg tatcactggc ctgggaagat atagacctta aagcgaggac
      481 tataaccatc cgccggaatt atacaaaact aggcgaattc actccaccaa aaactgatgc
      541 tggtaccgga agaacgattc atctagttca accagctatt gatgctctta aaagccaagc
      601 ggaaatgacc atgcttggaa aacagcattc tgtagaggta aagcagaggg aatatgggag
      661 aagtactgtg cataaatgca cttttgtttt tagtcctcag gtaataaaac agcggcagtt
      721 ttccggaccg cactataagg ttgactccat cagggagtca tggacaagta tcttaaaacg
      781 cgcaggtctg agacacagaa aatcgtacca atccaggcat acttatgcat gctggtcact
      841 tgccgctgga gctaatccta gttttatcgc aagccagatg ggccacacaa acgcacaaat
      901 ggtattcaat gtttacggag catggatgaa agacaacaat cacgaacaga tagaactcct
      961 taacaaaaga ctatctgaaa gtgtcccatg tatgccccat aagaaagtgg ggtaaaataa
     1021 aaacttgtaa aatcagttag tttaccctta atccctgtca cgttacgcgc gtggcagagg
     1081 cgttacgggt tgctgaaacc gcaacggaca gacggcggtc atcgactgtt caacgatgcc
     1141 gatattgacc ggatccgcga gatcaaacgc tggatcgaca acggcgtgca ggtcagcaaa
     1201 gttaaaatgc tgctcagtaa tgaaaatgtt gatgtgcaga acggctggcg cgatcagcaa
     1261 gaaacattac tgacctacct gcaaagcggc aatctgcata gcctgcgaac gtggatcaaa
     1321 gagcgcggtc aggattaccc cgcccagaca ctcaccacac atctgtttat tcctctgcgc
     1381 cgacggcttc agtgccaaca accgactctc caggcgctgc tggcgatcct cgacggcgta
     1441 ctgatcaact acatcgccat ttgtctggct tcggcacgta aaaaacaggg taaagatgcg
     1501 ctggtggttg gctggaatat tcaggatacc acccgtctgt ggctggaggg ctggattgcc
     1561 agtcaacaag gatggcgcat tgatgtcctc gcccactcgc tcaatcaact acgccctgaa
     1621 ctattcgaag gccgtacatt gctggtgtgg tgcggtgaaa atcgaacctc cgcccaacag
     1681 cagcaactca ccagttggca agaacaaggc catgatattt tcccactcgg catttaatga
     1741 ttcgttaaca aatgcgcttt actgtacaat cctttcgtta acataaggag tgcaatatgc
     1801 gcatagctaa aattggggtc atcgccctgt tcctgtttat ggcgttaggc ggaattggtg
     1861 gcgtcatgct cgcaggttat acctttattt tgcgtgctgg ctaagcgcct gcaccagcct
     1921 ttcaaacagg cggtctgcga tgatcgccgc cagtgccacc agtaacgccc cctggatcac
     1981 atacgcggta ttaaatccgc taagcccgat gatgatgggc gtacccagcg tgctggcccc
     2041 taccgttgag gcgatcgtcg ccgtaccaat gttgataatc accgaagttc gcacgcccgc
     2101 cagaatcacc ggagccgcca gcggtagctc gaccttacgc agtcgctgac cacgactcat
     2161 tcccatacct ttcgcaactt ctgtcacgct ggcatcaatc gctcccagcc cggcaagtgt
     2221 cgcctgcagg acgggcagca caccgtaaag gatcaaggcg ataatcgctg gttgcagacc
     2281 aaagccgatc accggaacgg caatcgccag cactgcgaca ggcggaaaag tctgtccaac
     2341 ggcggcaata gtttccacca gtgggcgaaa ttccgcgccc cacgggcgag tgacagcaat
     2401 tccggcacca gtgccaatga tcaccgcaaa caaactcgaa attcccacca gccagaaatg
     2461 agccagtgcc agagctgcaa aactttcttg ctgataaacg ggtcgtggca gttgtgggaa
     2521 caaggcagca aacagcggct ggctgtaagg cagccagaaa atcagtgcca caaacaaagc
     2581 aatgagccag aacagcggat cgcgcaacat cttcatacgc ttacgcctcc tccagcagat
     2641 cctgaaaatg cagcgtgccg caaggctggc cctgcgtgtt caccaccggc agcacctcgc
     2701 atccccgcgc gacaaacagg gagagcgcat cgcgtagcgt catctcttct gccagtgcct
     2761 caccttctgc ccgttcttcg cgacgcacgt aatccgccac actacgtaac gaaagcaggc
     2821 gcacacccag ttcgctgcgc ccaaaaaact ggcggacaaa atcgtttgcc ggacgagtca
     2881 gcatcgtcag cggattgccc tgctgcacca cttcaccgtg atccatcaat accagatgtt
     2941 ctgccagccg tagcgcctca tcaatatcat gagtgaccag cacaatggta cgccccagca
     3001 aacggtgaat gcgcgtcatc tcttgttgca acgcgccgcg cgttaccggg tccagtgcgc
     3061 caaaaggttc atccatcagt aagacttgcg gatcggcagc cagtgcgcgc gccactccca
     3121 cacgttgctg ctgaccaccg gaaagctgat gcggataacg ctcacgtaaa tttgactcca
     3181 gccccagtag cgccattaat tcgtcgatac gatcgtcaat cctcgcccgc gaccattttt
     3241 gtaattgcgg cacggtagcg atgttttgcg ccacgctcca gtgggggaac aggccaatag
     3301 attgaatggc atagcccatc cggcggcgca actccagtac tggcagcgag cgaatttctt
     3361 ctccggcaaa gcggatcact ccgctgtcat gctccaccag gcggttaatc attttcaggg
     3421 tggtggattt gccggagcca gatgtgccaa tcagcaccga aaaactccct tcctgaaaat
     3481 tgagattgag atcgttaacg gctttttgtg cgccgaacag tttgctgaca tggccaaatt
     3541 caatcattac gtttcacctt cagcagtgcg ataagcaaat cgaacagcgc gtcggtaagc
     3601 accgccagaa caattaccgg gatcaccccc agcaacacta aatcaatggc gctgcttagc
     3661 agcccctgga aaaccagcgc accaaaaccg cctgcgccga ttaacgccgc aatcaccgcc
     3721 atacctacag tttgcaccat caccacccgc aggctgcgca gaaataccgg taacgccagt
     3781 ggtaactgaa catgcaggaa tcgctgcgcc ccgctcatgc ccatcgctct ggcgctctcc
     3841 agcacatcgc gcgggatctg gttcaaaccg actaccacgc cgcgcaccag cggcagcaag
     3901 gcatagagca ccagcgcaat cagtgcgggt gtcattccgg ttcctgctat gccgagcgtc
     3961 cccagccacg gaaaggccgt aaccagcgcg gcaagcggcg caatcaacag gccaaagagc
     4021 gccaccgaag gcacggtctg aatgacattg agcagagaaa aaattgcccc ctgccgagca
     4081 gtggaaaagt agcaccagat gcccaacggc acaccaatca ctaacgcagg cagcaccgca
     4141 ccaaacagca acgtcagatg ttgtgccagc gcgtcgtcaa acacatcctg acggttggcg
     4201 tattctttca ttagtgagag atcgttaagc gtgccggagt acagcaacca cagcggaata
     4261 atggcaatct gcatatgcaa caaccagcgc cacagcggat gcgtggagat tcggcggatg
     4321 gcatcactac aggccagcaa tgccagcgcc gcggccagcc agaaaccact gccgaggctg
     4381 gtacgcgcca gcgcactgcc attttgcgcc agttgggtcg ccgcctttcc tgctccccac
     4441 accagcaata cgaagacgaa ttgcgccaga atgagtgcaa aaatgctgcc ttttttagcg
     4501 ggaataaaac aggctgtcag ccaggcgcaa ccaacgccca ccagcatcca gatcgtttgc
     4561 ggccacagtt gccagagatg acgcccttca cccgagacta aacgattagg cgcgtaactg
     4621 ataaacggca gcgccgctgc gattgccgtc agcaacagca gcagcgccag aacaggatta
     4681 atacgtagat aagtcacggg aaattacttc gtccaccctt tttgtttcag gtagtcggca
     4741 gccacttttt tggcatccag tccttccact gcaatgctgg cattcagttg ctgcaatgtt
     4801 tttgcatcga ggctggcgaa gactggctgt agccactgtg ccatttgcgg atactctttc
     4861 agcaccgact cacgcaccac tggtgcaggc gcgtagatag gttgcacgcc tttcggatcg
     4921 cttaaggttt gcagccccag cgccgcgacc ggaccgtcag tgccgtaagc cattgcggca
     4981 ttaacgccag aagtttgctg ggcagcggct ttaatcgtta ccgccgtgtc gccgccagcc
     5041 agtgacagta actgatcctg atcgagttta aagtcgtagg ctttttcaaa cgcgggtaac
     5101 gcatcggcgc gttcgataaa ctctgccgag gctgccagtt tgaaggtgcc gccctctttc
     5161 agataacgac tcaggtcggc aagcgaagtg agtttgtttt tctctgccac atcctgacgc
     5221 acggcgatgg tccaggtgtt attcgctggt gcgggcgtca gccagattaa cttgttttgc
     5281 tctgcatcga gttttttgac tttctcgtaa ccttgctgcg cgtttttcca tgccgcatcg
     5341 ttttcatctt taaagaagaa agcgccatta ccggtatatt ccggatagat atccagttca
     5401 ccggaagtaa tcgccccgcg caccacagga gtcgttccga gttgcacttt attcaccgtc
     5461 ggtacgccgt ggctttccag cacctgcaag atgatgttgc cgagtagcgc gccttcggta
     5521 tcgatttttg aaccgacttt aacgggggaa gccgcctgta gcggcaggct cacggctgcc
     5581 aacataacca gtgaacctgc ccagaccttt gagagtggca tgatgctttc ctcattcttt
     5641 tactgttgtt ttcagcgaat taagagaaaa gcatagttga taatgacaga gttagcctga
     5701 gcggaaggat tcagttgcag aatcagataa atacttaaag ggcatatttt gcccggcgtt
     5761 tcttgccgga tgcggcgtaa acgccttatc cggcctacaa aggtcgaaaa ttcagcaagt
     5821 taagatatga acatcaggca aagggcgcaa acgtcgcgcc cttactaaag cattacagca
     5881 actcaaactc gcctttctta acgcgtgcgg aatcagtgcc gataaagaca ttgaacttgc
     5941 caggttcggc gtcatatttc atctgttgat tccagaactt cagcgcctcg atatcaatcg
     6001 ggaagctgac agtctgagtt tcgcccggtt tcagggtgat tttctcaaag cctttcagct
     6061 gcttcactgg acggctcatg gaagccgtca catcctgcaa gtacatctgt actaccgtgg
     6121 ccccttcgcg tttaccggtg ttcgtcacct gcacgctggc ggtcactttg ccgtcacgct
     6181 tcatggtcgg cgcagaaagt ttcacatcag agacggtgaa agtggtatag ctcagaccat
     6241 agccgaacgg ataaagcgcc ccgttagctt catcaaaata acgcgaagtg tatttgttcg
     6301 gcttgtcggc attatacgga cgaccggtat tcagatggct gtagtacacc gggatctgcc
     6361 cgacagaacg cgggaaggac attggcagct tgccggacgg gttgtaatcg ccaaacaata
     6421 catcggcaat tgcattaccg ccttcagtcc ccgcaaacca ggtttccaga atcgcatcag
     6481 cctgctgatc ttctttcacc agcgccagcg gacgcccgtt catcagcacc agcaccagcg
     6541 gtttaccggt ggctttcagc gccgcaatca agtcacgttg gctttgcgga atagtgatat
     6601 cggtacggct ggaggcctcg tgcgccatcc cctgtgcttc accgaccaca gccaccacca
     6661 catcagattg tttcgccgtc tgcaccgctt catcaatcat ctcttgcggc gagcgcggat
     6721 cgactttgac cgcttcttca tactgattca ggaaatcgat aatgcctttg tcactggtaa
     6781 cgttcgcccc tttggcatac agcactttac cgttttcacc gacggcattt ttaatcccgg
     6841 tcagtacggt cacggattga tcggcaacac cggctgcgga ccagctgccc atcacgtcac
     6901 gtttactgtc cgccagtggc ccaaccaccg caatggtggc cgattttttc agcggtaacg
     6961 tttcgagacg gtttttcagc aacaccaggc tttcgcgcgc cacttcacgc gcttctttac
     7021 ggtgcaggcg gctttcggca ttggtatcca ccgggtcaga ctctttcggc cccaaatggc
     7081 tgtatgggtc gttaaacaat cccatatcat atttaacgtt cagtacatgg cgggcagcat
     7141 cgtccagctc ttccatcgtc actttgccgg atttgatcaa cccaggcaga tacttcgagt
     7201 aatactcgtc gctcatactc atgttgattc cggatttcag cgccacgcgc accgcatctt
     7261 ccgggtctgc cgccgtgcca tgtttaatca gctctttgat tgcaccgtga tcggaaacgg
     7321 tgatgccttt aaagccccac tggtcgcgca gaacatcttt cagcagccag gaatcggagg
     7381 tggctggcgt gccgttcagc gagttcagcg ccaccatcac cgcgccgctg cctgcgtcca
     7441 gccccgcttt gtacggcggc atataatcat taaacaggcg ctgcggactc atatcgacgg
     7501 tgttgtactc tttaccgcct tctaccgcgc cgtatgcggc aaagtgtttg acgctggtca
     7561 tcaccgagta gcgatctgcc gggcttttac cctgcatcgc ttccaccatg gttttaccca
     7621 ttgttgaggt gagatacgta tcttcgccaa aaccttcgga agcacgtccc cagcgcggat
     7681 cgcgcgagac atcgaccatc ggtgcccagg tcatattcag gccatcatct gccgcttcat
     7741 aagcagagac acgtccgacc gttttcactg catcgaggtt aaaagacgag gccagaccga
     7801 ggctaatcgg gaacaccgtg cgctgaccgt gcagcacgtc gtaagcaaag aaaagaggaa
     7861 ttttcaggcg gcttaattcc atcacctgat cctgcatggc gcggatatcc tgacgggtta
     7921 cggtgttgaa aatcgcccca acctgaccgt ctttgatcat ctcgcggatc gcctctttcg
     7981 ggttatccgg accgacgctg attaagcgca gctgaccaat tttctcatca actgtcattt
     8041 tcttaagcag ttcggtgacg aacgcatccc gcgcttcggg cgttaatgga tggttgccga
     8101 ataaatcatc cgccagtgct ggctgcaggg ccagactcac cgcgattcct actgaacata
     8161 gccatttcat atggattttt cttcctctgt tgcccgacga ggcagcgaaa atggtgcaaa
     8221 aaccgtagtt tgccataagc atgatggaga gagaaaaaga atgctcagtt tattgtctga
     8281 attttcaaaa tattcactcg ctgaattgtc atacaaggcg ctattctagt ttgtgatatt
     8341 ttttcgccac cacaaggagt ggaaaatgtc ttccatgaca acaactgata ataaagcctt
     8401 tttgaatgaa cttgcccgtc tggtcggtca ttcacacctg ctcaccgatc ccgcaaaaac
     8461 ggcccgctat cgcaagggct tccgttctgg tcagggcgac gcgctggctg tcgttttccc
     8521 tggctcacta ctggaattgt ggcgggtgct gaaagcctgt gtcaccgccg acaaaattat
     8581 tctgatgcag gctgccaata caggcctgac cgaaggatcg acgccaaacg gtaacgacta
     8641 tgatcgcgat atcgtgatca tcagcaccct gcgtctcgac aagctgcacg ttcttggcaa
     8701 gggcgaacag gtgctggcct atccgggcac cacgctctat tcgctggaaa aagccctcaa
     8761 accgctggga cgcgaaccgc actcagtgat tggatcatcg tgtataggcg catcggtcat
     8821 cggcggtatt tgtaacaact ccggcggctc gctggtgcaa cgtggcccgg cgtataccga
     8881 aatgtcgtta ttcgcgcgta taaatgaaga cggcaaactg acgctggtga accatctggg
     8941 gattgatctg ggcgaaacgc cggagcagat ccttagcaag ctggatgatg atcgcatcaa
     9001 agatgacgat gtgcgtcacg atggtcgtca cgcccacgat tatgactatg tccaccgcgt
     9061 tcgtgatatt gaagccgaca cgcccgcacg ttataacgcc gatcctgatc ggttatttga
     9121 atcttctggt tgcgccggga agctggcggt ctttgctgta cgtctagata ccttcgaagc
     9181 ggaaaaaaat cagcaggtgt tttatatcgg caccaaccag ccggaagtgc tgaccgaaat
     9241 ccgccgtcat attctggcta acttcgaaaa tctgccggtt gccggggaat atatacaccg
     9301 ggatatctac gatattgcgg aaaaatacgg caaagacacc ttcctgatga ttgataagtt
     9361 aggcaccgac aagatgccgt tcttctttaa tctcaaggga cgcaccgatg cgatgctgga
     9421 gaaagtgaaa ttcttccgtc cgcattttac tgaccgtgcg atgcaaaaat tcggtcacct
     9481 gttccccagc catttaccgc cgcgcatgaa aaactggcgc gataaatacg agcatcatct
     9541 gctgttaaaa atggcgggcg atggcgtggg cgaagccaaa tcgtggctgg tggattattt
     9601 caaacaggcc gaaggcgatt tctttgtctg tacgccggag gaaggcagca aagcgttttt
     9661 acaccgtttc gccgctgcgg gcgcagcaat tcgttatcag gcggtgcatt ccgatgaagt
     9721 cgaagacatt ctggcgttgg atatcgctct gcggcgtaac gacaccgagt ggtatgagca
     9781 tttaccgccg gagatcgaca gccagctggt gcacaagctc tattacggcc attttatgtg
     9841 ctatgtcttc catcaggatt acatagtgaa aaaaggcgtg gatgtgcatg cgttgaaaga
     9901 acagatgctg gaactgctac agcagcgtgg cgcgcaatac cctgccgagc ataacgtcgg
     9961 tcatttgtat aaagcaccgg agacgttgca gaagttttat cgcgagaacg atccgaccaa
    10021 cagtatgaat ccggggatcg gtaaaaccag taagcggaaa aactggcagg aagtggagta
    10081 aaaattacgg atggcatagt atcgccatcc gtcattcact taatcgttct gtgccgtctg
    10141 ccccgccgcc gccatttggg cggctttttg ttttttatag ctcaacgctg ctgctggaac
    10201 aggcatcact ttaccggttt caatccaggt acgcaggcgg ctggcatcgg caaaatgggt
    10261 atatttgcca aacgcatcca tcactaccag cgccaccggt ttattattga taaccgtacg
    10321 catcaccaga caatggcccg ccgcattggt aaaaccggtt ttggttaact gaatattcca
    10381 gttatcgcga tacaccagat ggttagtatt gcggaacggc agcgtatacg tcgggttaga
    10441 gaaggttgcc atatcttccc gggtagtact taactgcccg atcaacggat attgtttgct
    10501 ggcaatgagc agtttggtta agtcacgggc agttgaaacg ttatgcaccg acaatccggt
    10561 aggttcaaca aagcgcgtgt tgttcattcc gagcgatttc gctttcgcat tcattgcctt
    10621 aataaaggct ttgtaaccac cgggataatg gtgtgcaagg cttgccgccg cgcggttttc
    10681 tgaagacatc agcgccagca acagcatatc tttacggctg atttcgctat tcagtcgtac
    10741 gcgcgaatag acccctttca tctccggcgt ctggctgata tccactttta gtttttcatc
    10801 cagcggcagt cgtgcatcca gcacaaccat cgcggtcatt aatttgctga tagacgcaat
    10861 cggacgcacc agatccgggt ggttcgagta gatcactttg ttggtattca gatcaacaat
    10921 catcgcgcta ccggaggcaa tttcaggttg tgaagcggtg gtagcggctg ccgttttcgc
    10981 aacggcctgc ggtgccaaag gcacagccag catcagggcc aggctaaata aagaaactcg
    11041 aaatttcggc atgatgagca ttcagatagt ggttcacgcg cacaggttgc gcaccgccgg
    11101 agtaaggatt tactgaggct agcgacgcca tcataacgag caaaaagtgc gatcgtcaaa
    11161 ggagaatcgt gaggaaatgc tgcattgctg acatttacgc cagcaatgca acgtcaaaga
    11221 actttctaga acaaacgata accgtagccc catagtataa cggttagggc gagcagcact
    11281 tccagtacca gcacgccaat cgccagcgtc gaactagaga agctaaggcc ttcctctttg
    11341 ttgatattca ggaagctcgg aataccaagg taaagcaggt agccggtgta aaacagcgcc
    11401 accgtgccga ccagcgcaca caaccagacc agtggataaa gcgccaccag accgcttaaa
    11461 aacagcggag ttgcaacgta gcccgcgaag accatgcagt gcgcaagtga cggacgctgc
    11521 ggataattac gcgccatcca ccagatgacc cgccccatta ccgcaacccc agccagcata
    11581 acgccataaa acaggacagc cagcgccagt ccggtaaacc aggataactt caggatagtg
    11641 ccatcgccaa aattccagcc aatctgtgta gtgccaatga aggcgcaaat caccggaatc
    11701 gccgccatca gcaaaacgtg gtgggtgtaa tggtgagaaa tcgtttcgtt ttcgcgatta
    11761 atcacctgca tttcacgatc gggatgggaa aacagtcccc agacatggct cataccgccc
    11821 ccttgttgtg agttcatgaa cctgacagtt caagtataag tcagcttgtg attatttttt
    11881 gttcgctcat gtaatttgca ccgtctgatg tacgtccttt ttcagcgtga atgatttcca
    11941 gggtgtatgc ttacaggcaa ccaaagggag acagactggc ctatggatct caatacactc
    12001 atctcacaat atggttatgc cgcgctggtg atcggtagcc tggcggaagg tgaaaccgtg
    12061 actttgctgg gaggcgttgc ggcacatcag gggctattaa agttcccgct ggtggtactt
    12121 tctgtggcgc ttggcggcat gattggcgac caggtgcttt atctgtgcgg gcggcggttt
    12181 ggcggcaagc tgttacgccg tttctcgaaa catcaggata aaattgagcg ggcgcagaaa
    12241 cttatccaac gccatccgta tctgtttgtc attggtacgc gctttatgta tggctttcgg
    12301 gtgattggcc cgacgctgat tggtgccagc cagctgccgc cgaaaatctt tctgccgctg
    12361 aatattctcg gcgcatttgc ctgggcgttg atttttacca ctattggtta cgctggtggt
    12421 caggtgattg cgccgtggtt gcacaatctc gaccagcatt tgaagcactg ggtctggttg
    12481 attctggtcg tggttctggt ggtgggcgtg cgctggtggc tgaaacggcg cgggaagaaa
    12541 aagccggatc atcaggcgta aaaccattgc cctgggtaag gcgttcgcgc cgcatccgac
    12601 atccggtgca ccatgcctga tgcgacgctt gtgcgtctta tcatgcctac caaaatttta
    12661 cccattgaaa taaccacgca cctcactgcg ctattctggg ttgaactgcg gattcgccaa
    12721 cataaagccg ccatccacta tcaacgactg cccggtggtg taatttgcgc cctctgagca
    12781 cagccacgct accaggctgg caatctcatg cgttgcgcca aaacgccgca gaggaatcga
    12841 aggctccgcg tcgggcttca cgtcgctgtc atccatgcca ttcattggcg tggcgatcgc
    12901 cccaggcgcg actgcgttca ccaaaatctt atgcctgacc agctccagcg ccatcgtttt
    12961 ggttaacccg ccgagcgcat gtttagcggc tgtgtaagcg ctggcatccg gcagcggcgt
    13021 atgttcatgt accgacgtaa tgttgatgat acgaccgccc tgcccttgtt tcaccatctg
    13081 gcgggccgca atttgtgagc ataaaaatgc accatcgaca tcaacggtaa aaatcttgcg
    13141 ccactcatcg aaagccatat caagaaacgg cgctttggtc attgcgcccg cattattcac
    13201 cagcacatca atacgcccca gccgttgaat gagtttctcc agcgcctgcg caccttctgg
    13261 cagtttaccg agctccagct gcacgatctc cgcacgtacg ccgtggctaa ctacctcacg
    13321 cgcggtatct tttgcccctt cttcatctga gtgccaggta atgccaatat caaacccctg
    13381 ctgcgccagt aataacgcgc actctttgcc gatccccgaa tcggaggcgg taataatcgc
    13441 aacctgtgcc atcaagttct ccacttaacg ctgaataaac gttaagtata gaaggcgcat
    13501 atcatcagcg tttgtaaccc ccgcccaacg caccagtgag ttgaatagaa gcatccagcc
    13561 actgcccttg caataacagg ccattggccc gctcacgcag cgcggggatt ctggcttcgc
    13621 tgacgcggga accagcaatg atgcccgcgt taaagcgcgc ctgcgcaaga cctaccacac
    13681 gcaaggcatc gcgctcaatt tgcgcctgat gctggttttt ctccgcaagt gtctgaacct
    13741 gactggctgc ccgcgccacg tcattcaccg cttcaaccac cgctttgttg tagctggcga
    13801 tagacaagtt gctttcggct tttgcgatat tgagattggc gttaagacga ccactatcga
    13861 aaatgggtag cgtcaggcct gccgtaacgc ccatttgctg cgcggaatga cggaacagat
    13921 cgcttaagtg caacgcatcc tgttgcagga aggccatcag gttgatgtca ggataaaatg
    13981 ccgcttttgc cgcatcaatg gtgcttagcg atgactcaac gtaccagtgc gccgcctgca
    14041 aatctgcccg ccgggccagt aaggagtacc ccagttcatc aggaagctgg cttgccactt
    14101 tcggcaacgc gaccggttta agcttcaatg actttgtctg gttatttgta agtgcgctta
    14161 accgtgcctc aataattttc attttccccg cgacatcgtt gagctgctgc cgggttttgc
    14221 tggcattaat atcggtttcc acaccttcaa ctgaagaagt aatcccgttc tgatatagct
    14281 ggcgatcagt cgcgataatg gtgttctgct ctttttctat ttgctgcaag accgtgttta
    14341 acgccgcctg ggtttgccac tcccagtaca ggcgggctac gctgccagcc agcaattggc
    14401 gggtttgctc gcgttccgcc gcccgtgctt taaccgtacc caggcgggca gtaacctccg
    14461 cccgattctt tccccagata tcgagatgcc agcccgccgt taagccaaaa gtaccgttgg
    14521 tgtaccacgg gccggtcgta cctgcggccg gatcgttcag agcaaacggc cccattaagc
    14581 cttctgccga cattttttgc cgctccatat ccgccgaaaa gtcgatctgc ggaccatcct
    14641 gagtggcaac tgccttcgcc tgggcttcag ctagctgaat gcgctgttca gccacctgca
    14701 tatccggtgc gttctgtagt gcattgttaa ttaatgaagt gagctgatta tcgtgatact
    14761 ccagccacca ttggctgtct ggccaaccat ttttcagcgc cgtgggtaat gcggtgtcaa
    14821 cttgtgcagc gggcgtttgc tggcttaacg cctggcgggt ttcatgcata ggcgcacacc
    14881 cggccagcat cagtaacagc ggaaaacagg cgatggctgg ataaaaggaa tcacgattca
    14941 tgggggaata atcaggtaag aaacggtgcg cggagattac cgtgtgttgc gatatatttt
    15001 ttagtttcgc gtggcaatac atcagtagca ataaaacgac atacccagaa aaatatagac
    15061 taagtaaatg atatcttccg atttatctta atcgtttatg gataacggca aagggcttcg
    15121 ttttttccta tacttattca gcactcacaa ataaaggaac gccaatgaaa attatactct
    15181 gggctgtatt gattattttc ctgattgggc tactggtggt gactggcgta tttaagatga
    15241 tattttaaaa ttaattaatg tcatcaggtc cgaaaataac gagaatattt cagtctctca
    15301 tcctgttgcg ctcctgtcat gtgcattgct tcatataatc actggcgcaa ggagcgcgca
    15361 gggggcggcc aatcgccgcc gccccctgca cccccgggct ctggcgaaca aaatcgccgc
    15421 tgcgcggtgc cctcggctta tcccttacgg ctaccgggtc gggcgcgagg taacatccct
    15481 gtaaaacgcg ccctcagccc acatccatgt gggctgcccc ggccttcagg gaacgcctcg
    15541 gcaattttga cgccaccaaa caaccgtgcg gcctattgat aaagagctaa cacattgtca
    15601 aaaaacatca ctatggtttt ttagagtttc tcgatatcaa ttgcctgaat agcccttgca
    15661 atatcagggg aattattcaa cacccgaaca tgctgaaata attccgttgc ttcatcgtat
    15721 tctttacgca aataactcaa ccactgttta atccgcgcaa cgtgatataa cccggtatcg
    15781 ccctgctttt ccagacgggt atatttttgc agcaaagcaa ccacctccgg ccacggcatt
    15841 cgcggttcgt tatattttac tacccggctc aggttgggaa tattgagcgc cccgcgacca
    15901 atcatcactg cgtcgcagcc gctgatcgca atgcattgtt gcgcgctctg ccagtcccag
    15961 atttcgccgt tggcaatcac cggaatattc agccgctggc gaatctcgcc aatcgcctgc
    16021 cagtcaatat gctccgcgcg gtaaccctgc tcttttgtcc gtccatgcac caccagctcc
    16081 gtagcaccag cctgttgaac cgcatcggcg atttcaaatt tcttctcacc gctgtcccag
    16141 cccagacgca ctttcacgct gacgggcaaa tgcgccggta cagcttcacg catcgctttt
    16201 gcaccctggt agatgagttc aggatctttg agtaacgtcg ccccgccgcc gctgccgtta
    16261 accgctttcg acgggcagcc gcaattgaga tccacgcccc aggaacctaa ttccaccgca
    16321 cgggcggcgt tctctgccag ccattgtggg aactgaccta acaactgaac gcgcaccagc
    16381 gtaccggatg gtgtccggct ggcgttttgt agctcagggc aaatgcgatg aaagactttt
    16441 accggcagca gttgatccac cacgcggaca aactcggtga tgcacagatc gtagtcgtta
    16501 acttcggtca gcaattctcg caccagagag tcaagcacac cctccatcgg agccagtaac
    16561 acacgcatat cttcacccgg aaaaaaatga ggcgctatgt tagcgcctat ggtcaacgga
    16621 ttaaaggtct gcaaagaggg attgctgcaa actgtaatcc ctctctatgc ttgatgtatg
    16681 cctgagttcg ttgcgcattg cgtaatgccg gggagcgaaa aaataagcgc tgatttctta
    16741 atgtgatcgg tagcacgttt tacgaattaa ttgtatgatg aatccatctc atctggggtg
    16801 ttgattatga gtaagacact gaacattatc tggcaatatt tacgcgcttt cgtcctgatt
    16861 tatgcctgcc tgtatgcagg cattttcatt gcttccctgc taccggtaac cattccgggc
    16921 agcatcatcg ggatgctgat cctgtttgtc ctgctggcct tgcaaattct tccggcaaaa
    16981 tgggtcaatc cggggtgcta cgtactgatt cgctatatgg cgctattgtt tgtgccgatt
    17041 ggcgtaggcg tcatgcaata ttttgatttg ctccgcgcac agtttggccc ggtagtggtt
    17101 tcctgtgcag tcagtacgct ggtggttttt ctggtggtga gctggagttc acaactggta
    17161 cacggtgaac gtaaagtcgt aggtcagaaa ggatcagaag aatgatggcg aatatctggt
    17221 ggtcattacc gctgacgttg attgtctttt ttgccgcccg caaactggcg gcacggtata
    17281 agtttcccct gcttaacccg ttgctggtag caatggtggt gatcattcct tttttaatgc
    17341 tgaccggcat ctcttacgac agctacttta aaggtagcga agtgctaaac gatctgctgc
    17401 aaccggcggt cgtcgcgctg gcctatcctt tatatgagca gctacaccag atccgcgcgc
    17461 gctggaaatc gatcatcacc atctgtttta tcggcagcgt ggttgcgatg gtgacgggga
    17521 cttccgtggc attgttgatg ggcgcttcac cggaaatcgc cgcgtcaatc ctgccgaaat
    17581 cagtcaccac gcctattgca atggcggttg gcggcagtat tggcggtatt ccggcaatca
    17641 gcgcagtttg cgtgattttc gtcggcatcc tcggcgcggt atttggccat acattgctta
    17701 atgcgatgcg tattcgtacc aaagctgcgc gcggtctggc aatggggaca gcctcgcacg
    17761 ccctcggtac ggcgcgctgc gccgagctgg attatcagga aggtgcattt agttcgctgg
    17821 cgctggtgtt atgcgggata attacttcgc tgatcgcacc gttccttttc ccgattattc
    17881 tggcagtaat gggctaaaat ttgcgatgcg tcgcgcattt ttgatgtaag tttcacgcgt
    17941 tgcataatta atgagattca gatcacatat aaagccataa cgggttcgta aactgttatc
    18001 ccattacatg attatgaggc aacgccatgc atccacgttt tcaaaccgct tttgcccaac
    18061 ttgcggataa cttgcaatct gcactggaac ctattctggc agacaagtac ttccccgctt
    18121 tgttgaccgg ggagcaagtc tcatcgctga agagcgcaac ggggctggac gaagacgcgc
    18181 tggcattcgc actacttcca ctggcggcgg cctgtgcgcg tacgccattg tcgaatttta
    18241 atgttggcgc aattgcgcgc ggtgtgagcg gaacctggta tttcggtgcc aatatggaat
    18301 ttattggtgc aacaatgcag caaaccgttc atgccgaaca aagcgcgatc agccacgcct
    18361 ggttgagtgg tgaaaaagcg cttgcagcca tcaccgttaa ctatacgcct tgtggtcact
    18421 gccgtcagtt tatgaatgaa ctgaacagcg gtctggatct gcgtattcat ctgccgggcc
    18481 gcgaggcaca cgcgctgcgt gactatctgc cggatgcctt tgggccgaaa gatctggaga
    18541 ttaaaacgct gctgatggac gaacaggatc acggctatgc gctgacgggt gatgcgcttt
    18601 ctcaggcagc gattgcggcg gcaaaccgtt cgcacatgcc ttacagtaag tcgccaagcg
    18661 gtgtcgcgct ggaatgtaaa gacggtcgta ttttcagcgg cagctacgct gaaaacgccg
    18721 cattcaaccc gactctgcca ccgttgcagg gggcgttaat tctgttgaat ctcaagggtt
    18781 atgattaccc ggatatccag cgcgcggttc tggcagaaaa agccgatgcg ccgttgattc
    18841 agtgggatgc cacctccgca acgctgaaag ctctcggctg tcacagtatc gaccgcgtgc
    18901 ttctcgctta agcctggtgc cggatgcggc gtgaacgcct tgtccggctt gccagccctc
    18961 tcctggtgtc gaaattcccg gcaaacagtt tgccgtttct tgcgcaaaac cagcgggtaa
    19021 agtagcctga tggaaatttt ccttagatcg agtctcctgc atgttaaagc gcgtgttcct
    19081 cagcctgtta gtcctgatcg gcttgctgct gttgactgtg ctcggcctcg atcgctggat
    19141 gagctggaaa accgcgcctt atatctacga cgaattgcag gatctcccct accgccaggt
    19201 cggcgtggtg ctcggcacag caaaatatta tcgtactggc gtaattaatc agtattatcg
    19261 ctaccgcatt caaggagcga ttaatgccta taacagcggt aaggtaaatt atctattact
    19321 gagcggcgat aacgcattgc aaagttataa tgagccgatg accatgcgca aagatttaat
    19381 cgctgctggt gtcgacccat cagatattgt tctcgattac gcaggctttc gtacgctgga
    19441 ttccatcgtg cgtacacgca aagtgttcga tactaatgat ttcattatta tcacccaacg
    19501 tttccactgt gagcgggcat tatttattgc gctgcatatg gggattcagg ctcagtgtta
    19561 tgccgtaccg tcaccgaaag atatgctgtc agtacgtatt cgcgaatttg ccgcccgttt
    19621 cggtgcgctg gctgaccttt atatttttag acgtgaaccg cgttttttag ggccgctggt
    19681 ccctattccg gctatgcacc aggtgccgga agatgcgcag gggtatcccg ccgtcacacc
    19741 cgaacagtta cttgaattac aaaagaaaca aggaaagtag ttatggatgt acagcagttt
    19801 tttgtcgttg ccgttttttt ccttatcccg atattttgtt tccgcgaagc atggaaaggc
    19861 tggcgcgcag gcgcgattga taaacgggtt aaaaatgcac cggaaccggt gtatgtctgg
    19921 cgagcaaaaa atcccggact ctttttcgct tatatggtgg catatatcgg cttcggaatt
    19981 ttatctatcg gcatgattgt ttatcttatt ttctatcgtt aatattttct cttttctgtt
    20041 aattattgag aataattatt acttcactca ataagctgcg gatataacat tctgaaccgc
    20101 agctatttgt gaatcctttc acagtttaaa tttccccaca cgcttagcct taatatcagc
    20161 acgcaatatt tatttagcgt tcgccttaat tactcatagc attaaggaag atcacatgcc
    20221 gcaacaaaat tatctggatg aactcactcc ggcttttacg cctttactgg cgattaaaga
    20281 agcctctcgc tgtttattat gtcatgacgc tccctgtagt caggcttgcc cggcacagac
    20341 cgatccgggg aaatttattc gctcaatcta ctttcgtaat tttaaaggcg ctgccgagac
    20401 aattcgcgaa aataatgccc tcggtgccgt ttgtgccaga gtgtgcccga cggagaaatt
    20461 atgccaaagc ggttgtaccc gtgccggtgt tgatgctccc attgatatcg gccgcttaca
    20521 gcgttttgtt actgattttg aacaacaaac cgggatggaa atttatcagc ccggtactaa
    20581 aacgctcggc aaagtcgcga ttattggcgc aggtcctgcc ggattgcagg ccagtgtgac
    20641 actgacaaac cagggttatg acgtcacgat ctatgagaaa gaagcacaac ccggtggctg
    20701 attgcgtaac ggcattccgc aattccggtt accgcaatca gtactggatg cagagatcgc
    20761 ccgtattgaa aaaatgggcg tgaccattaa gtgcaacaac gaagtcggta acacactcac
    20821 ccttgagcag ctgaaagcag aaaaccgcgc ggtactggtc accgtggggt tatcaagcgg
    20881 ttccgggcta ccgctgtttg agcatagtga cgttgagatt gccgtcgact tcttgcaacg
    20941 tgcacgacag gcgcaaggcg atatcagcat tccacaaagc gcattaatta tcggcggcgg
    21001 tgatgtcgcg atggacgtag ccagcacgct gaaagttctc ggctgtcagg cggtaacttg
    21061 cgtagcgcgt gaagagttag atgagtttcc ggcaagcgaa aaagagttta ccagcgcccg
    21121 ggagctgggc gtttcgatca tcgatggatt cacgccagta gccgtggaag gcaataaagt
    21181 cacgtttaag catgtacggt tatcgggcga actgacgatg gcggcagata aaattattct
    21241 cgccgtcggt cagcatgcca gactagatgc ctttgcggag ttagagccgc agcgtaacac
    21301 catcaaaaca caaaattacc agacccgcga cccgcaagtc tttgctgctg gcgatattgt
    21361 tgagggagac aaaaccgtgg tctatgccgt gaaaaccggg aaagaagccg ccgaggcgat
    21421 tcatcactat ttagagggag cttgctcatg ttaacgaaag atctttcgat tactttttgc
    21481 ggcgtgaagt ttcccaaccc gttctgcctc tcttcttcgc cggtaggcaa ctgctatgag
    21541 atgtgtgcca aagcctacga cacaggttgg ggcggtgtgg tgtttaaaac gatcggcttt
    21601 tttatcgcca acgaagtctc gccgcgtttt gatcatctgg tgaaagaaga taccggtttt
    21661 atcggcttca aaaatatgga gcagattgcc gagcatccgc tggaggagaa tctggccgcc
    21721 ctgcgtcggc tgaaagaaga ttacccggac aaagtattga tcgcttcgat catgggggaa
    21781 aatgagcagc aatgggagga gctggcgcgc ctggtgcaag aagctggcgc ggatatgatc
    21841 gagtgtaact tctcctgtcc gcaaatgact tctcatgcga tgggtagcga tgtcgggcaa
    21901 agcccggagc tggtagaaaa atattgtcgg gcagtgaaac ggggttccac gctgccaatg
    21961 ctggcgaaga tgacgccgaa tatcggtgat atgtgcgaag tggcgctggc ggcgaagcgc
    22021 ggcggcgcag atggcattgc ggcgattaac accgttaaat ccatcaccaa tatcgatctt
    22081 aatcagaaaa tcggtatgcc gatcgttaac ggaaaatcaa gtatttccgg atattccggt
    22141 aaagcggtaa aaccgatcgc cctgcgcttc attcagcaaa tgcgcaccca tccagaactg
    22201 cgcgatttcc caatcagcgg tatcggcggc attgaaacct gggaggatgc ggcggagttt
    22261 ttattgctcg gcgcagcaac gttacaggtc accaccggca ttatgcagta cggctatcgc
    22321 atcgtagaag atatggcgag cgggttatcg cattatctcg ccgatcaggg atttgattcg
    22381 ttgcaggaga tggtaggtct ggcgaataac aatattgtcc cggcggaaga tttagaccgc
    22441 agttatattg tctatccccg tatcaatctt gacaaatgtg ttggctgtgg acgctgttat
    22501 atttcctgtt acgacggcgg tcaccaggcg atggaatgga gcgagaaaac ccgcacaccg
    22561 cattgtaata ccgagaaatg tgtgggttgt ctgctttgtg gtcacgtctg cccggtgggt
    22621 tgtattgagc tcggggaagt gaagtttaag aaaggcgaga aagaacaccc ggtaacgttg
    22681 tagaacccgc gtcacatccg gcatttggtt cctgttgccg gatgcggcgt gaacgcctta
    22741 tccggcctac aaaaccacac agatcatgat gttatgtgcg tttatgaccg aatgcggacc
    22801 acattcacat catttcttac gcgcgtattt cagtgaatcc agcgctacgg cgaagataat
    22861 aatcgcccct ttgatgatgt actgccagta tgggtttacg ccgatatacg tcaggccata
    22921 gttgatgacg gtaaaaataa tcaccccggt caccacgcca atcaccgtcc ccacaccgcc
    22981 gctgaacgat acaccgccta ccacgcacgc cgcgatagca tccagctcat acataaagcc
    23041 gaggttgttg gtggcagagc cgatacgtcc ggcttctaac atcccgccaa aggcatagaa
    23101 cacgccagac aacgcgtaga tcatcagcag gttcaggccg acgttgacac cagatacttt
    23161 tgccgcttcc gggttaccgc caatggcaaa aatgttctta ccgaagcggg ttttgttcca
    23221 caacacccag acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag agagacggaa
    23281 actccccagc gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa aaccagaaat
    23341 tggcgacgcc ccgacaaagt cgtaatagag cgagttgatg ccatagacga tgatcatcgt
    23401 gccgagcgtg gtaatgaacg gcgtcacgtt gagataagcg ataatcagac cgttgatcaa
    23461 accgatcacc gcaccaatgg cacagacaat cagaataacc agcgcaatcg gcatcgtcgc
    23521 catttccggg aacactttgt tggcgttatc catggactgc aataatgtcg cagccaccac
    23581 tgccgccagc cctacctgac gaccagcaga aagatcggtc ccctgggtga caattaaccc
    23641 tgccacaccg agcgcgataa taatacgcac cgatgactgg gtgagaatat tacttaagtt
    23701 caacagactt aaaaatgttg ggtcctggaa aataataatc gccagcaaaa ctaaaagaac
    23761 gacgtaaata ccgccctctt tcaggtaagt aagaaaactt ttcttattta acgcactcat
    23821 gggaagcccc taatcttaaa ggtgcaaaga cgcaagacgc agaatttcgt tttgcgttgt
    23881 tgtttttgta tcgacaattc cggaaacgag accattgctc atgaccagaa tacggtctgt
    23941 tatccctaac aactcaggca tttcagagga gataataata atccccttgc ctttcttcgc
    24001 cagttcggca attaactgat aaatttcaaa cttcgccccg acatcaatac cgcgcgtcgg
    24061 ttcatcgagc attaatattt ctggttgcgt tagtagccag cgaccaataa tcaccttttg
    24121 ctgattacca ccggagagcg aaccaatttg cgtccgatga cccggcgttt ttacccgcat
    24181 cgaatcaatc acccactggg tatcgctttt catccgcgag ttatccagta aaccaacttt
    24241 atttttgtag ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca gataggcata
    24301 aattcccgtt gagcggcgct cctcagttac cagtgcaaat ccgtggttta tggcttcgtt
    24361 ggcattatgg ttattgatct gtttgccgtg caacgtaatg gtgccagccg atttctcgcg
    24421 aataccaaat aacgtctcaa caatatcggt acgtttcgcc cccaccagcc cggcaatacc
    24481 gaggatctcc cctttatgca gatcaaacga gacatcgcga atcgacggct ggcgcagtga
    24541 cgttaggtta cgcacctcga ggatgacttc gcccggcttg ttttctttgt caggaaaacg
    24601 ctgattaaga gaacgcccaa ccatcatggc gatgatcttg tccatcgtca gtcctgccag
    24661 cggttcggtg gcgatccact gaccgtcgcg caatacggta acttcatcac ataactggaa
    24721 gatttcttcc attttatgcg agatataaac aataccgcag ccgcgctctt ttaatttacg
    24781 aataatagtg aacagatgat tgacctcttt ttcggttaac gaagaagtcg gttcatccat
    24841 aatcacaatt ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct gcatttgcga
    24901 aacggataat gtgccgacgc gcgcacgcgg atcgatatca atatccagtt catcaaaaat
    24961 cgctttggtt tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg tgggatatcg
    25021 ccccagccac atgttatcca tcaccgaacg ttgtaatacc aggtttaact cctggtgtac
    25081 catcgaaata ccattttcca gtgcctcttt ggcggaatgg aaatcgatct ctttaccctg
    25141 gaataaaatg gtgccggagt ctttttgata aataccaaac aggcatttta ataatgtcga
    25201 ttttcctgca ccgttttccc ccattaatgc atggatagaa tgtggccgga attttaaatt
    25261 aacgttatca agtgccttaa caccaggaaa ggacttgttg ataccgctca tttccaacaa
    25321 gtattccccg gaagacggag tcgttgagct gaccatataa ttttaccttg ttggccatac
    25381 aataagggcg cagtaaacga ctgcgcccaa tcagtcttat ttcttgctga attcagccag
    25441 gttgtcttta tctacgccaa cataaggtac gcggaccact ttgttgtcga ttttccagtt
    25501 ggtgccatca gccgcacctt tgccatcggc caggtttttc gccagatcaa aggtcgcttt
    25561 cgcctggttg ttagcatcgt tcagtactgt gcccgccagt gcaccggatt tcaccagcgc
    25621 cagcgcttct ggcagcgcat cgacgccaaa caccggaatg ctggacttgt tgtgtgcttt
    25681 cagtgcttct accgcgccca ttgccatcgc atcgttgttg gcgataacca cttcgatttt
    25741 gttggcgttc gggccagaca gccaggcgtc catcttatct ttcgcctgag cggtgtccca
    25801 cattgcggta tctaactgta actgttcagt tttgatgcct ttatcgttca attctttaat
    25861 cacgtaagtg gtacgtgctt ctgcatccgg atggcccggt tcacctttca gcagtacgaa
    25921 ctgaatctga ccgtctttgt tcagatccca accctgattc gccgcccagt gtttagcaat
    25981 caaatcgccc tgaataatgc cggactcttt ggagtcagtg ccaacgtagt aggctttgtc
    26041 gtagctatcc agcgccttac gagacggttc tttgttgaag aaaaccaccg gcacgttttg
    26101 cccacgcgct ttctcaatca ccgtacccgc agctgccggg tcaaccaggt tgattgccag
    26161 tgccttcacc cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg actggtcatt
    26221 ctgagaatca ttcatcagca gctgaacatc tggcgcggct ttcgcatctt gctcaatagc
    26281 cttgcgcact acagacataa agttatcgtc gtacttatag attgttacac caatgcgagt
    26341 atcagcagcg tgtgcagcgg caccgaataa catgctggcc atcacagcag acagggttaa
    26401 caccttctta ttcatggtat ctccggtttt tcttatgcag ggtagtgctt gagataaatg
    26461 ctcggcgggg cagtagagtt aatgaagtgt tactgaacgc cgaagctcac ttttttaaat
    26521 tcgttcttcc atgcccggta acgctccaga aaacggcttt aattgttgtt atgacgctat
    26581 tacctcggtg aaagtgatta ttcactgtta catacgggtt acaacgttaa aacggtgcaa
    26641 tcatagctat cacattgtta agatactgtg aaatcactca cagattgaaa gcggttacat
    26701 cgcctgattt gttgagttag tgatcgccgc cgcattctgg cgcgttgcga cagaatggcg
    26761 acgcactaac gtcggcataa aacagtggct ggcacgagga tcaatattgc ctgctgcccc
    26821 ctgcaaggcc agttcggtgg ctaatttagc cattgaagca atgggataac gcacggtcgt
    26881 taattgcggg tcggtgtaac gggcgatggg aatatcatcg aaaccgatga ttgagagatg
    26941 taacggaatc gcaatgccat tatcttttaa tgctgtcagt gcgccagcgg ccatattgtc
    27001 gttataagca aatatagcgg taagttgtag attgcgcccc agcagttcaa ccatcgccgc
    27061 ctcaccgccc ggcatgtccg gcgtaccagt gccaatccag ctttccggcg gaataatatc
    27121 ctgctctttc aacgcactca tccagcctgc tttacgcatg gcgtcatctt caatgccgtg
    27181 actggaagaa agataaccaa tacgttgatg accgttattc agcaacatgc gcgtcgccat
    27241 tcgggcaccg ctgagattat ccaggcaaac gcaacgatgg gcgtaccccg gcacaacgcg
    27301 gttgattaac accataccgg gaatgttatc cataaattgc gccagttcat cgtcactcaa
    27361 tgcttttgag tgaacaatca acgcattaca acgctggcga attaacacct caatggcgtg
    27421 acgctctttt tccgcttcat gatagctatt gccgattagc acgtatttct gatgctgctg
    27481 agcgactaga tccaccgctt ttaccagcgc gccgaaaaac gcatcagaaa cgtccatcac
    27541 caccacgcca atggtgtcgc taacctgagt tgccagcgcc tgggcattgg cgtttggccg
    27601 ataatccagc tcactcacgg ctttcattac tgcttcacgc gtgtcggcac tgaccagcgt
    27661 gctgttattg agcacccggg aaaccgttgc cacagagacg ccagcctaac gcgctacatc
    27721 acgaatggtg atcatattca ctacctgtta tgagattgca gtaaatgact gcttgctggc
    27781 ggctattttg tcagcactga atacaggact tcgtgaatcg agtcacagca atggaaacgg
    27841 ttacagccgt tttgttaacg aatgtgatcc agatcgttat cttgatgttt tagatattgc
    27901 cgttccggca gcacgcaaag ttaactgacg ccagagccat tccaccggcc cctggcggaa
    27961 gtaacgcagc cagataacag agaagaggat attcgccagc cataccggaa taacaaacgc
    28021 cagcagctcc aggcggtcaa aatgcataaa caaaccgagg tggtaaaaaa gcatggtaca
    28081 aatcagcgtt tgcaatagat agttggtcag cgccatccgt ccgacgcagg cgatcgcaag
    28141 caccagctta aagcggctca attgcggcca gaagccataa aacagcgacg catagccaat
    28201 cgcctgaaac ggcgcactca gttcccgcgg catttgaagt aagaaggcac accagcgata
    28261 cgcccagtcc agctgccatt gcagggcgat ggcaggaagg ttaatggtca ccccaatcgc
    28321 caccagcaca aaaccagtac gacgatagtg acgtaagctg aactgccctt tcagccagcc
    28381 gctgcgcatc agcgccgcac caatgagcat catccctgcc agttgccagc catactgtgc
    28441 gcccagtgcc agtaaactgt tgccaacacc atcggcacga ttactgatcg cttcaacgcc
    28501 gccgtgaagc ttccagtatt tttcatacaa aatagccgat gcatccggcg tccaggtgcg
    28561 gctggtctgg ctgtcggaaa tcagccccaa taacagcaaa acgccaagcc ccaccagata
    28621 aagcatgacg cctgtattaa acaggctttt taccgatggc gcatcgcgca ccagccgcca
    28681 gcagattaag cccaccagcc cgtaagccag cagaatatcg ccgtcccaga acaataaacc
    28741 gtgaataaaa cccagcaaga ctaacagcgt taaccgcgac tggatccagc gtctgccacg
    28801 gggcagcaac atttgcaggc ccgcaccaaa cagcagcgca aaaagcgtga ggaatttcac
    28861 ctggccgatg agatcgagaa atgcccaggt ccatgcatcc tgcggcgtaa tagcgccgta
    28921 ccaggcggga ttgagataag ccgcctttgg tagcccaaag gcgctgatgt ttaatagcag
    28981 gatccccaga atggcgacgc cgcgaacaaa atcgagcgtt acgttgcgct ccatggttcc
    29041 tgccttttaa tcagttgtga tgacgcacag cgcgcagaaa ctcgtggcgc gtattctgac
    29101 tggatttgaa caatccacca agagaggtcg ttgttgtggc actggttgca tcgcggatgc
    29161 cacgcgcttt cacgcagtaa tgcaccgcat caatggagac agccacgtta ttggtgccca
    29221 gcagcgtttg tagcgcaata agaatttgct gcgtcagacg ttcctgcacc tgcggacgct
    29281 gggcaaagaa ctgcacgata cggttaattt ttgacagacc gatcaccgaa tctttcggga
    29341 tataagccac cgtcgctttg ccatcgatgg taacaaaatg gtgttcacag gtactggtca
    29401 gagtgatatc gcgcacggtg accatttcat cgaccttcat tttgttttca atgagggtga
    29461 ttttcgggaa gttggcgtaa tccagaccgg agaaaatttc atcgacatac attttagcga
    29521 tgcgatgcgg cgtttccatc aaactgtcat cagccaggtc gagattcagc agctgcatga
    29581 tttcggtcat atgaccagca ataaggcttt tgcgcgtttc gttatccatt tcatgcacgg
    29641 gcgggcgcag cggtgtttcc agtcctcgcg caactaacgc ttcatgaacc agggccgctt
    29701 ctttactgag tgatggcatt tatgatttct cctgcaggtg tgacgcctcc gccctgcgtg
    29761 ggggcaaagt tattaagctg atttacagcc tgattattgt gcgtgaggcg gcgcacataa
    29821 tccagtattc acagcgataa ttattgtaat tgccgctgac tttcatcagc agatgttaaa
    29881 acatcgttat gcaaatacgg aagtgaaagt tactcacagc acattgaata aacggtatga
    29941 tgaagaaatt gcaaacaaca caacaaggag ccacgcatgg aaatgctcga agagcaccgc
    30001 tgttttgaag gctggcagca acgctggcga cacgactcca gtaccttaaa ctgcccgatg
    30061 acgttcagta tctttctccc accccctcgt gatcacaccc cgccaccagt gctgtactgg
    30121 ctttccggat taacctgcaa tgacgagaac ttcaccacca aggcgggtgc ccagcgcgtg
    30181 gcggcagaac tggggattgt actggtgatg ccagacacca gcccgcgcgg cgaaaaggtt
    30241 gccaacgacg atggctacga tttaggccag ggcgcaggct tttatcttaa tgccacgcaa
    30301 ccgccgtggg cgacgcatta ccggatgtat gattatctgc gcgatgaatt accggcgctg
    30361 gttcagtcgc aatttaatgt cagcgaccgc tgcgccatta gcggtcactc aatgggtggt
    30421 cacggtgcgc tgattatggc gctgaaaaat ccgggtaaat acaccagcgt ttcggccttt
    30481 gcgccaattg tgaatccgtg cagcgtcccg tggggaatca aagcgtttag cacctattta
    30541 ggtgaggaca aaaatgcatg gctggaatgg gatagttgcg cgctgatgta tgccagtaac
    30601 gcgcaggatg cgatcccgac gcttatcgat cagggcgata atgatcagtt tcttgccgac
    30661 cagttgcaac ctgcggtatt ggcagaagcc gcgcgccaga aagcgtggcc gatgacgctg
    30721 cgtattcagc cgggatatga tcacagttac tacttcatcg cctcttttat agaggatcac
    30781 ctgcgcttcc atgcgcagta tttactgaag tgaaagtccg cccggttcgc cgggcatctt
    30841 ctcatcagaa gcgataatcc actgccataa agtaacgacg tccgtcttcg ttatagctgt
    30901 agtcgtcacg actgagatcc ttgtcgccaa ggttcagcac gcctgcgcgc agtttgacgt
    30961 ctttagtcac ctgccaggcc gcgccggtat tccagatggt ataaccgccc ggtgttttag
    31021 ccgtcgcgct gtcggcgcgt ttctgcccgg tatagtgacc agaaacatag aatgaccagt
    31081 cttccagcgc cagcggtttc cagtccagcg taccgttagc agtatggaac ggcagatcgg
    31141 atagcggttt gttttcgccg ttgctgacat cacgaccatc gttgtaggtg tagttgatcg
    31201 acagtttcca ttcatcgttg aacggaattt tcagttcggt ttccacgccc tgaatacgag
    31261 ctttgttaac gttgtagtag ctaaataccg gtatgcgccg tccgttagcg cccgtctcaa
    31321 aaccaacaaa gttttggtag cccggtgcag cgttgacgtc agacgtacgg ctgatgctga
    31381 tacgatcttt cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc
    31441 cttcttcacc catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat
    31501 ccgggctacc cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa
    31561 gttgcaacag agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt
    31621 cggtggcgtt ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat
    31681 cgtccatacg cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca
    31741 caaacagcgc gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg
    31801 catcgctaag tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg
    31861 tcagcggcaa cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt
    31921 tgccagggtt tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat
    31981 cccaacgccc attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg
    32041 agtcggaatc acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat
    32101 tcggtgtcca ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg
    32161 tttctccggt atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc
    32221 tgccgtaagc tttcattccc agcacaccat caattaatgg tccactggta aagaactgac
    32281 cgttataggt atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg gtaacggtac
    32341 ccgaccattt ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg
    32401 aaccgtacag cgacgacatc gggccacgga ccacttcaat acgttcgatg gaatcgaccg
    32461 ggatccagtt cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttcacgcgtt
    32521 taccgtcgac gagaatcagg gtatagctgc tgtccagacc acgaatacta acgcccttac
    32581 ggttatcccc ttcgttcgtc agttgtacgc caggcacttc tttgaggaca tccttcagat
    32641 tctgtaccgg ttttcgctgc agatcttcct gggtaatgac gctgatactg gcgggtgcat
    32701 ctttaaggtt ttgttcaacg gaagatgcag tgacaaccat cgtttcgcca tcgtcatcga
    32761 ccgctaacac aggccataca caagaaatag cggacaaaca cagcccgacc cgtacgaaag
    32821 ggttcaacct aaacattcca tatctccatg aggtaactac gaaaataaaa tgggtatcgc
    32881 tcacatcttc tttacgtccc cttgcgtacg gcagcatcgc ggtggactta tttgtatgca
    32941 ggtgatcatc ccagaaagcc ggaccagcct ctgatttgtc agcctttttt gattgtagcg
    33001 taacgataat gcaaacgata acagttatca atccaaatgt taaattttat atccgcatga
    33061 tttgtcaggg aaatggaatt gaaaaagccc tctcggttga gagggcttag caaggaaggg
    33121 aggaaactta tttcttatcg ttctgcggga acttcatttc gctgtagcgt acgaagtgag
    33181 ttcctttaat cagcttgtag ccgaaccaaa taatcaggaa cagcgggata ccaatatacg
    33241 ttgccgctac gccgccccag tcaatggtat ctttcaggaa cgcttcgtag ttctggccca
    33301 aagtgataat cagacacaga atgaatgcga agatcggccc cagtgggaag aaacctgaac
    33361 ggtacggcag atcgttaatg tcgtgtccct gcaatacgta accgcgacgg aagcgatagt
    33421 ggctaatggc aatccccagc caggcgataa aacccgtcat cccggaggtg ttcagcagcc
    33481 acaggtatac cgtctggttg ccaaacatgg aggtcaggaa gcacaaaccg gcaatcaccg
    33541 tcgtcgcata cagcgcatta cgcggcacgc caccacgcga caatttagcg aaaatgcgcg
    33601 gcgctttacc gtcacacgcc agggtgtaca gcatacgagt agacgcatac ataccggagt
    33661 tacctgctga cagcaccgcc gtcagaataa ctgcgttcat caccgccgcc gcagagagca
    33721 gacccgcgtg ctggaacacc aaggtgaacg gactgacgct gatgtcttta acatcgttac
    33781 gcagcaggct cggatcggta tacggaataa tcaggctgat aatcaggatc gcgaacacat
    33841 agaacaacag gattcgccag aatacctgac gtaccgcgcg tggaatgttt ttcgccggat
    33901 cttcggactc gcctgcagca ataccgatca gctcggttcc ctggaaagag aagccgacaa
    33961 tcatagctac gccgatcatc gccgcaaaac caccagcaaa cggcgcttcg ccgattgtcc
    34021 agttgctcca gcccgcaggc tgcgcgcctt tgaagatacc gataatcatc agcacgccaa
    34081 cgatgataaa gacaataact gtcgtgactt tgatcagtga aaaccagtat tccgcttcac
    34141 caaagccacg aactgagatg tagttcagca ggaagataac gccgaggaac aacgcactcc
    34201 agatccagcc cggtgtatcc gggaaccacc agctcatgac cagctgtgct gcaaccaagt
    34261 caacggcaat agtcaccgcc cagttgtacc agtagttcca gcccagcgcg aagccaaagc
    34321 cttcttcaac atagttctga ccgtaagtgg caaacgaacc ggaaaccggc atatatgcag
    34381 ccaattcacc gagactggtc atcaggaagt aaaccatcag gccaatcagc atatacgaga
    34441 gcaatgcccc gcccggacct gcctgagaaa tcgttgcgcc agaggcaaca aaaagacctg
    34501 taccgatgga accgccaatg gcaatcatcg tcaggtgacg cgcctttaat tcacggcgta
    34561 agcccggcgc ttctgtagtt ttagtttcgg aacccataca aaaatgctat ccatcttaaa
    34621 aatgaggcgc gattgtagca gacgattggc aatccttccg gcagaaatac acggttataa
    34681 gacaccttca tgatcgccca gggattataa gtaaagcagc caatctcttt tctggcgaat
    34741 gcatgctgag ggcgctgcgt cacgaaatcg acacagcacc agcatgttct tgtacagcac
    34801 cttaacgcgg tacattcgcg ggatcgcaat agtccagaaa gcgccgtagc gcgttggaaa
    34861 ggtgtttttg ccgatgatgt atacgccaca acgtacgcat caggcgcggg agagggaccg
    34921 caacttcact taatgtgcct gcctgcaatt gatcttcaat cacacgtcgc gacaggcaac
    34981 taattcccaa cccatgacgc accgcatgtt tgattgcctc ggagttacct aattccatcg
    35041 ccatctcaaa cttcggtaaa tgtgacagca acagataatc gacaatctcc cgcgtgccgg
    35101 aaccgcgttc acgcaggatc cacggcgcag cggccagctg ttctaaggtg accggaccac
    35161 gagccaacgg cgaagtcggc gcggcgaaaa ccaccagctc gtcttccagc cacggttcag
    35221 aaatgatttc agtgctgtgg cacggtcctt caataaagcc aatatcaacg cggaaatcca
    35281 gcaccgcttg catcacgtcc tggctgttcc caacgctaag ttcaatcggc aactgcggat
    35341 aatcatggcg ataacgggcg ataactgcag gcagaatgta gttaccgatg gtactactgg
    35401 catagatacg aatcgcgccg ttgtcttcgc gaaacagttg ttcgatttca accgcctgtt
    35461 caagcaatgc caacgcacgc ggatagagca gccgcccgtg ttcattaaca accagtcttt
    35521 tccccacgcg atcaaacagt tgcacgccaa gctgcccttc caggtcggtc aaggctgcgc
    35581 tcactgctga ttgcgacaac gccagcatca ccgacgcctg ggtggttgat ccacttttca
    35641 atacttctgc aaaaacttcc aactgccgga gggtgatgtg catagtcgct taccacttat
    35701 aaagattaat tataaatata taatcaattt tatttttaaa ccagttagtc gtaaccttat
    35761 gcccggttaa aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg
    35821 tggcatttta ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt
    35881 ggttccattc ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg
    35941 gggatggttt taggcaacac catctatccg cacatctgga aaagctgtga cggtggtgtg
    36001 ctgtttgcca agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg
    36061 ttctcgcaaa ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt gacgctgtcc
    36121 agtaccttcc tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc
    36181 agctggttga tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag
    36241 tcggtagtga aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc
    36301 gggaccgtcg cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt
    36361 ccggaaactt tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg
    36421 gcaggtcatg ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc
    36481 gtgatgatgc tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg
    36541 gcgaacagcg gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgtc
    36601 gttgccatct ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg
    36661 ctggatacct tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc
    36721 gcgctgaaaa aagctggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg
    36781 attgttggtg gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca
    36841 tcttgctcct gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc
    36901 tcgcatgaaa tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat
    36961 tcgcgccgcc gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg
    37021 gcgtgccgca ccgctcacga cgcaaaccat cgatgaattc aaagccgcct gtgaaaaata
    37081 tcactacaca tcggcgcaaa ttcttcccca cgacagttat ctgattaacc tcggacatcc
    37141 ggtcactgaa gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga
    37201 acaactgggg ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga
    37261 agaggattgc cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg
    37321 tgtaacagcg gtgattgaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga
    37381 acatctcgcg gcgattatcg acggcgtgga agataaatcc cgcgtcggcg tctgcattga
    37441 tacctgccat gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgaaaaaac
    37501 gttcgctgat tttgcccgta ttgtcggctt taagtatctg cgcgggatgc accttaacga
    37561 tgcgaaaagt acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat
    37621 cggtcatgat gcgttccgct ggatcatgca agacgaccgt ttcgacggca ttccgctgat
    37681 ccttgaaacc atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca
    37741 aactgaaaaa gcggtggcct gatcgatgaa cgacagggaa aagcagattc ttaaaatttt
    37801 acggcgtaac ccgctgattc aacagaatga aatcgccgat attctgcaaa tcagccgctc
    37861 acgtgttgcc gctcatatta tggatttgat gcgcaagggt ttgattaaag gcaaaggata
    37921 tatccttacc gagcaggatt actgtgtggt tgtgggtgca atcaatatgg atattcgcgg
    37981 aatggcggat atccgttacc cgcaagcagc ctctcatcca ggttctgttc actgttccgc
    38041 aggcggcgtc ggacgtaata tcgcgcataa cctggctctg cttggtcggg atgtgcattt
    38101 aatatcagct attggcaacg atttttacgg cgagacatta ctggaagaaa cgcgccgcgc
    38161 gggcgtgaac gtttcaaact gcattcgtct gcatggacac agcactgcaa cttatctcgc
    38221 tatcgctaat aagcaggaag aaactatcct ggcaatcaat gacacacata ttctgcaaca
    38281 gctaacgcct caactgctca atacttcacg ggacttgatt cgccatgctg gtgtggtgct
    38341 ggctgattgt aatctcacac ctgaagccct ggagtgggtc tttaccattg ctgatgaaat
    38401 tccgatgttt gtcgataccg tttccgaatt taaagcgaac aaagtaaaaa actggtacag
    38461 ccggatccac actctgaaac caacacaaaa tgaactggaa attttgtggg ggcaaccgat
    38521 taaagacgac aacgatcgca tcagagctgt aaattcgctg catcaacaag gtgttaaacg
    38581 catttttgtt tatctgaaag atgaatctgt attttgcagc gataaggatg gagaacaatt
    38641 tttgctgacc gcgccagcgc atacgacagt agacagtttt ggtgctgacg atggttttat
    38701 ggcgggcctg gtatatagct ttcttgaagg atacagtttc cgcgacagcg cccgttttgc
    38761 ggtagcctgc gcggcaattt cgcgcgccag cggcagctta aacaacccta ccctgtctgc
    38821 cgataacgcg ctttcattag tgccaatggt gtaacaatgt tgccggattc gacgcttgac
    38881 cgcatcttat ccggctcata aattgcgcac tacgctaaac caataaagaa tcctgcaata
    38941 gtagcactca tcaggttaga aagtgtcgcc gccgccagcg cgcgtaaacc aagttgggcg
    39001 atttccggcg cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac cccgatagaa
    39061 ccaaagttag cgaaaccgca caacgcgaaa gaaataatcg ccacggtttt agcatccaga
    39121 gtgccacccg tttgcagata tggcgagaaa ttgagataag cgacaaattc attgatcgcc
    39181 agcttctgcc caatcaaact cccggcaaga tttgcatcac tccagtcaac ccccatcacc
    39241 cacgccaatg gggccaacag gtaacctaaa atcgactcca gcgaagcatg ttcaaaacca
    39301 aaccagccgc caacaccgcc gataataccg ttaatcaacg caattattgc aacaaatgcc
    39361 ataacaactg tcgctacacc ggcggcgatt ttcagcccgg tcattgcccc tgtcgcagcg
    39421 gcttcaataa tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa agaaacctgt
    39481 gaagattccg tagccgggct taacaggcgg gcaaacaaga tcccgccagg gatcgccatt
    39541 aacgatgccg ccagcaaata ttccacaggt acgcccagtg cggcgtaacc aatcattgtc
    39601 gaaccagcaa tcgaggccat gccactacaa atcgctgtaa ataattcatt gcgattcaga
    39661 cgatcgataa agggtttgac gatcgccgga atttcgtttt gcccgaggaa aatggtggtg
    39721 accgcgacga atgactcgat cttgctgata tttaaagctt tctggaagat accgccgaga
    39781 atgcgaatta agatccccat cacaccgatg tagtagagaa tactcaccag tgcagtgacg
    39841 aagataattg ccggtaatac cctgaaacca aagataaatc ctgcgccatc aaataaggta
    39901 tccattttcg gtccgaccag agaaccgaag ataaatgcgc tacctgcgtc gctgtacgcc
    39961 atcactttgt ggacgccaaa agcgactttt tctgcgaccc aacgccctgg cggtaaccaa
    40021 agcataatcc cgccaatcac gacctgtaac actaacgccg cgccaacggt acgcaggctg
    40081 atcttcttct tgtttactga cagtaaaaac gcaattgcca gcagtaccac cattcccaga
    40141 acacttctca tgacatccat agtgattatc tcttcatgtc attttgggcg acggctttct
    40201 gcccatcaga aatagccggg cctgcatctt gcgatactgt cccggcttgt tatcaacact
    40261 taatgggttt ttatgtagcc gcgtacgcac tcttcgacta atccccagaa ccagtcggta
    40321 tcaatagtga tgccgacttt agtattggcg ggcttgccaa gaacgcccag ctcgtcgcag
    40381 acggtacgcc catagcaagg gccactgttg acgtccactt cgacgtacat ctcctgggtt
    40441 ttaatgccat cagggttaat cagataaccg atgcaggtgg cgtcgtgcac cgggccgcca
    40501 gccaggccgt agttttcgaa ctgcgttttg agagtgaagt tcatgatgtc gctgaacatc
    40561 tctccggcag ggccgcctgc cctttccatt cgtgcaatta cgtccggggt gcaaacggtc
    40621 tggttggtaa gatcgaggcc catcatcact aatggaacgc cggaggtgaa caccacgcgt
    40681 gcggcttccg ggtcggcaaa gatattgaac tcggcagatg gggtgaagtt gcctgtaccg
    40741 taagcgccgc ccattagcac aatttcacgg attttgggca ggattgcggg ttgcatacgc
    40801 attgccaccg cgatatttga aagcggacca accggcacca gagtgatatc gccatcgctt
    40861 gccataaggg tatcaatgat gtatttcacc gcgtgggtac tttctgcctg gcgggtcagc
    40921 ggctcgaata ccgggccatc cagtccggtt tcaccgtgaa tattatcggc aacgatttgt
    40981 tgacgcataa tgggttgcgg catccccgca taaaccggaa cattaatctc cagtttctgg
    41041 caaacattca ggccattaat taatgtttta tcaagcgtct gattacccgc aacaatggtg
    41101 atacctaata aatcaatcgc cggatgtttc gccgccatca ttatagcaat agcatcatca
    41161 tgacccggat cacaatccag aataattttt ctcttttcca ttgtttattt cctctgtttc
    41221 cagttgcgtt attttttcta cagcaacgaa atttcgatcg ggccgatgat tgaatcttaa
    41281 caacagcgta cgaatgctaa atatgagaaa tctcatagcg gataaacatc gtgaaagaaa
    41341 tccacaataa tgatcttaag cagcaattga tgagtgaatc cgcgtttaag gattgctttt
    41401 taacggatgt ttcagccgat acgcggctgt ttcatttttt agcgcgtgac tacattgtgc
    41461 aggaagggca acagccgtcc tggctgtttt acctgacgcg aggccgcgcc aggctttacg
    41521 ccacgctagc taatggtcgc gtgtcgctga tcgatttctt tgccgccccc tgttttattg
    41581 gcgagattga gttaatcgac aaagaccatg aaccgcgtgc ggtgcaggct attgaagagt
    41641 gttggtgcct tgcgctccct atgaaacatt accgcccgct gttattaaac gacacgctat
    41701 ttttacgaaa actctgcgtc accttaagtc ataaaaatta tcgtaatatt gtttctttaa
    41761 ctcagaatca atcatttccg ttagtgaatc gcctggcagc atttatatta ctctcgcagg
    41821 aaggtgatct ttatcacgaa aagcatacgc aagcggcaga gtatttaggc gtttcttatc
    41881 gacatctttt atatgttctc gcgcaattca ttcacgacgg tttattaatt aaaagcaaga
    41941 aagggtatct aattaaaaac agaaagcagt tgtcaggact ggcgctggag atggacccgg
    42001 agaataaatt ctccgggatg atgcagtaaa aattattggc aatagcacga ttgccggatg
    42061 caacgcttaa taaggcgtac atccggcaat gaaactcaag ctaaaccaat aaagaacccg
    42121 gcaatggtcg cgctcatcag gttggaaagc gtcgccgccg ccagcgcccg taaaccaagc
    42181 tgggcgattt ccggcgcgcg ttttggcgaa atagccgaaa acgctccaac gacaacaccg
    42241 atagaaccaa agtttgcaaa cccacagagg gcgaacgaga taatggcaat ggtcttgaca
    42301 tccagcgtgc cgctcgtttg cagatagggc gagaaattga gataagcaac aaactcatta
    42361 atcgccagtt tctgcccaat caaacttccg gcaagatttg catcactcca gtccaccccc
    42421 ataatccatg ccaacggggc cagcacatag ccgaaaatgc tttccagaga gacattggca
    42481 aaaccaaacc agccgccaac tccaccaata atgccgttga tcagcgcaat gatcgctaca
    42541 aatgccatta ccaccgtcgc cacaccggcg gcgattttca gcccggtcat tgcaccgttc
    42601 gccgcagctt caataatgct ttttggcggt gtttcgctaa acgacagatt ttcaaacgta
    42661 acttgcgaag gttccgtcgc cggactaaga atacgtgcaa acaggatacc gccaggaata
    42721 gccatcagcg aagcggctaa caaatagtca atcggcacgc ccattccggc atagccaatc
    42781 atcatcgaac ccgcaataga cgccatgccg ctacaaatag ccgtaaatag ctcgttgcga
    42841 ttcatgcgat caataaaagg tttaacgatc gccgggatct cattttgccc gaggaaaata
    42901 gtggtgactg cgacaaaaga ttcgattttg ctgatgttga gggctttctg aaaaatactg
    42961 ccaagaatac gaatcagcag ccccattacg ccaatgtagt acagcaagct gataagcgcg
    43021 gtaacgaaaa taatcgccgg gagtacgcga aaggcgaaga taaagcccgc accgtcaaac
    43081 agaacatcca ttttgggacc gaccagcgaa ccaaaaataa aggcacttcc tgcgtcactg
    43141 taggacatca ctttatggac gcctaatgcc gcctgctcta ctgcccattt tcccggggga
    43201 aaatagagca tgatgccacc aatggcaatt tgcagcagca gcgcagcgcc aaccgtgcgc
    43261 aagctgatgc tctttttatt taccgacaac acaaaggcaa ttgccagcaa taccaccatc
    43321 cccgcaacac ttctcattat atccataatg attttccctt catgccagta aacccggcgt
    43381 cagcgccagg ttttggtatg tttgatgagt acgggcgacg gctttctgcc cgccagaaat
    43441 taacccacga gacgctgata ttctttggca atttcgctcg ccagaatggc gttattaaag
    43501 accagttgaa tattggattt caggctatca ccgccggtta gctccgcaac acgcgccagc
    43561 aggaacggtg tactctcttt cccaataaca ccctgttctt cggcttcagc aacggcttga
    43621 tcaattgctg cattgatggt ttcttctggc atcgcaaact gttccgggat cgggttcgct
    43681 accaccaggc cgccgttcaa accggtttgc cacttcactg ccatcgcgcg agcaatttcg
    43741 cgggcactgt caagacgaat gctgacttca aatgggctgg ttcggcagaa aaacgcaggc
    43801 agcgcttttg tctgataacc aattaacggt acaccgaagg tttctaaata ttctgttgtt
    43861 aatcctaaat cgagaataga ttttgccccc gcacaaacaa cagtgacatt ggtatttgcc
    43921 agttcctgta aatcggcaga aatatcgaag gtatgttccg ctccgcgatg cacaccacca
    43981 atacctccgg ttgcaaaaac tttaatccca gccagtgctg caataatcat ggttgatgca
    44041 acggtagtcg cgccattttt ccctgcagca acgacaaaag gtaaatcacg acgactaact
    44101 ttcgtcacgt tatgtccttc acggcccagc aattcgattt cttctttact taagcccact
    44161 ttcatcacac cgccaataat ggcgatagtg gctggtactg cgccctgttt acgaattgtt
    44221 tcttctactt caattgcggt ttgggcattt tgtgggaatg gcattccgtg agaaattatg
    44281 gtcgattcca gcgcaacaac cggtttttta ttttttaaag catcctgtac ttcagcggat
    44341 atttgtaata attcagggga cagagttaat tcagacattc tgtgttctcc actaacgatc
    44401 taacattggc aattgataat tcagggttgt tggtatattc acaggcaagt gccattgacg
    44461 aacatccctg cgcaaatcga acagaatcga taaacggcat accgtctacc caacaggagg
    44521 ccagccccgc catcatggcg tcgcctgccc cagtaacatt gatgacattc gttttaatag
    44581 gggccgacca cccactttca ccgttgatat cactgtagta aacgccatcc cctcccatgc
    44641 tcagcaccaa gcggttcagg ccgtgttgat gaaaccaggc ggcgaccttt gcgacatctt
    44701 cacgtccgga aagtgcaatt ccgctcaacg tttcggcttc aagacggtta ggttttaacg
    44761 tatggatttt gctcagatga tcgcggattt tgacgcattt ccatgccgat accggatcaa
    44821 taaatacagg cgtttccccg ctattttcca gaacccagac cagcgcctct tcactaaggt
    44881 tgcaatccgc gacaataacc tttgcaccgc gaataaactc ctgatgctgg gcaagaaatt
    44941 ctgcagaaat acagtcacta atactcatat catttatcgc gacaagcatt tctcctgtat
    45001 tatcgagcag tgataaatag ctggatgtat tctcacccgg aacaattaag catttatcga
    45061 cataaacacc ggattgatta gtttgcgcca gtaatgattg cccataaaaa tcaccaccta
    45121 ctgcacttaa aagccaggca tttttcccca gtaacgccag attatgagcg atattccttc
    45181 ccacgccacc tggggtaaat ttaatttttc cgggattcga atccgcataa ttcaatggat
    45241 gatgtgaata cccagccaca tcaatattcg ccgaaccaat aattactatg tagtcctttt
    45301 cgcgcatagc acgccccctg gcaataaatt gccaacgaag aaattgggta aaatagccat
    45361 tacagtcaat aagactatta gagcacgtgt tcagatttaa acacatgttc ataataagca
    45421 tatttaacgt aatgtaaatc gatcagaaaa aacgaaatcg gccagatatg aaggcggtca
    45481 caaagtttgt ctatttatgc agcaacaaaa gagaaagttg tgatgtgtaa cacacaatat
    45541 ggcaggcaag aaaaagggca gagatgtttc tgccctccag attgttgaca acgcgcgctt
    45601 tgttcatcct ggatgtggcg taaacgcctt atccgaccta caaaagcgca aaaaattcaa
    45661 tatattgcag ggttcatgta ggtctgataa gcatagcgca tcaggcaatt ttgcgtttgt
    45721 catcatcggg gcagagatgt ctcatccccg ataatgaaga taaattatgc gtttttttct
    45781 actatttgtg tttccgggcg tttcaggaag gcataggcca aacccgccac cagcgtaccg
    45841 gcaataattg ctaccaggta acccaatacc ggcgtaatag cgccagggat cagcagaaca
    45901 aacagaccac cgtgtggtgc catcagtttc gcaccaatcg ccattgagat tgcgccagtc
    45961 agcgccccac caacgataca gcacggtagc acacgcatcg gatcacgagc agcaaacgga
    46021 attgcacctt ccgaaataaa gcacagacca agtaccagag cggctttgcc accttcctgc
    46081 tgcgctttgt cgaatttgcg acgcgccacc attgttgcca gacccattgc cagcggtggc
    46141 accatacctg ccgccataat cgccgccatc gggccatagg tttgagtact cagcagaccc
    46201 acaccgaatg cgtacgctgc tttgtttacc ggaccgccca tgtcagtaca catcatgcca
    46261 ccgaggatcg cccccagcag aaccgcattc gcagtcccca tggtctgcag ccagtgagtc
    46321 aacccatcga gaatgccagc aaccggttta ccgattaggt agatcatcgc cagaccgacc
    46381 accagactgg aaattagcgg aatgatcagg atcggtttca gcgcctccat actctgtggc
    46441 agtttcaatt gcgtactgat taacttcgca atgtaaccag ccaggaagcc agcaattata
    46501 ccgccaataa agccagaacc ggtgctgacc gccagcatac cgccaatcag acccggtgtg
    46561 aggcccggac gatcggcaat ggagaaggca atataacctg ccagtaccgg caccatcagc
    46621 gcaaaggctg aaccaccacc aatctgcatc agcgccgctg ccaacgtacc cggctcttta
    46681 aacgcttcga taccaaaagc aaaagaaagc gcgatacaca gaccacctgc aacaaccatc
    46741 ggcagcatgt aagagacgcc cgtcagcaag tgacggtaag cgcctgcact ctctttctta
    46801 ccttcagtgg tcgctgtttg agctttgccc gctggttcat acggcgttgc ttcagcaacc
    46861 gctttatcca gttcctgcgc agttttcttc agcgccagac cggtagaggt gcgatacatc
    46921 ggtttaccag caaatttcgc cagatccact tcgatatctg ccgccacaat caccagatcc
    46981 gctgctgcga cttcttcagg ggtgattgca ttgcccgcgc caacagaacc acgggtttca
    47041 actttcaccc accagccacg ttttttcgct tcggtttcaa tggcttcagc cgccataaag
    47101 gtgtgtgcta cgccagtcgg gcaagcagtc accgcaacta cgcgtttcgg accgctggca
    47161 gcaaccggtg ctgtcgcagc gaccggcgca gtgtaaggtt tcgcatggcc tttggcttca
    47221 ctcaggaaca gctcagggtg cgcaactgcc cgggaaatat cacccagcca gacattttta
    47281 ccgttcagcg cgctgtcatt cgggatggaa tcaccgagaa caatcgccat ttctgcgtcg
    47341 ttcggattgt cgatgatttc cagttttgct tttcgcgccg ccgcgcccag cagggtcttc
    47401 gccatatagg cgcgtgcctg accgagatta gcgtcaataa tcagcagcgt tttcattatg
    47461 cctctcctgc tgtcagttaa aaggttgtaa gtcgacgcgc gccatcattg cggccaactg
    47521 cggacgatcg gtaataccca cattgctttg acttaccgcc agggctgcaa cagctgtcgc
    47581 cagacgcagt gtgtgttcac tggattcacg catcagcaag ccataaatca ggccaccaac
    47641 catagaatcc cctgcgccaa cggtgcttac gacatcgact gacggtggtt tggcgatcca
    47701 ttcgccggag gcattaaccc aaagcgcgcc ttcggcaccc agtgaaataa caacatgcgc
    47761 aataccttgt tcacgtagcg catgtgcagc ttcaatcaca tctttcattt caggcagttt
    47821 acggcctgcc cagatttcca actcgcggcg gttaggtttc accagccacg gtgccgcttt
    47881 caaacctgct actaacgctt cacggctact atcaaagata atgcaaggac actgactacg
    47941 caggcgagtc atccagtcgg tgaacgcttc cgggctgacg cctgacggta agcttccgct
    48001 gacacagacc atatcgaact gaccgagcca gctcagagaa tcagtcacaa agcgttccca
    48061 gtcggcgagg gtgacttcaa aacccgagaa gttgaagtcg gtcacttcgc cgtctttttc
    48121 cgtcagctta acgttaattc gggtgcgccc ctgtacaacc tggaaacggt tggcaatgcc
    48181 cagctcgctg aacagttgct gaaaaccatc ctgattgtct ttacccagga agccgccaac
    48241 ggtgacatca attcccaggt cttttaatac tttggccacg ttgatgcctt tacccgccgc
    48301 atgcagaccg gtggttttca ccaggttcac ttcgccgcgt tcaatttccg ggcagaaacc
    48361 aacaaggtca taagccggat taagggtgat cgtagcaaca cgtctgctca ttatgcgccc
    48421 tccccaagac cagcagcgat agcgtcgcca atggctttca gcgcctgttc agcatctgca
    48481 ccctgggcgg taaagcgtag gcgatgacct ttcttaacgc caagtgccac aactttcatc
    48541 agactacgtc cgtttgccgg tttgccggtg ccatcaaggt ttgtcacggt aatatcactg
    48601 ttaaattgtt taatggtatt gaccagcatg gtacctggac gagcatgcag gccgtgttca
    48661 ttgcgcacca caaactccgc gcttaacacg tcgtcggtcg gcgcatcatc gctggtcagc
    48721 agcgccagca acgttgccgc atccgctttc agcaagcggt cagctttatt gtcgagcaat
    48781 aaatcagcga gacgcttaag aaccgcgatg ggctgatcgt cattcatcgc cacactcacc
    48841 agcatggctg ccgtttcgcc gtccacatca aaagcatttg ccgcacggct taccgcaatc
    48901 gcgctacgca gattgccttc ggcgctatcg ctcagccaga taccctgtcc gagattcagc
    48961 ggttgttcat tgatggcttt ggtgacgaaa gtggcgtcaa ctgcccccac ctctttcaga
    49021 cgcgcagcgt tcagcgcctg aagagtcagc agatcgctgg cgacgatatc cagcgtcagc
    49081 atttcgttgt cgagcttcag ctgctcactc tgcttttcgc ccatcagtaa tgcgcgaagt
    49141 tcttctgctg ttgttgctga cttcagttgt tcagcaacgg aatcatcgct cagtacgtgg
    49201 gtcagctggc gtagcaggcc cagatgttca tccgagctgg cagcaatacc gattgccacg
    49261 tacgctacct gaccgtcacc ccaggtgacg ccttccggga actgaaatac ctgaacgccg
    49321 gttttcagca cctgatcgcg ggtgtcggta gtgccgtgtg gaatagcaat accattgccg
    49381 aggaacgttg aggtttgctg ttcgcgcgcc agcatgccat tgacgtagcc ttctgctaca
    49441 ttaccggcct gcaccagcgc cgcagcgacc tggcgaatcg cctcttcttt gtctccggcc
    49501 ttttcgcccg gatggatgtc ctgtacggat aactggaaca tagttctcct ctcttgctga
    49561 attgaaacga ttcagcctct atgagaaaaa aagcgccaac ctggcttagg gttaaagaca
    49621 agatcgcgct gaaacgtttc aagaaagcat aatacttctg tttcagcacg caaggaaagc
    49681 cgtaaagtta tgagcaaaag tttgatgtgc tgcacaattt tttgtcattt ttcctaattg
    49741 ctgatgggaa aatccgttat cagcaatttc atttcagcag cgtcagcagc caatcagcaa
    49801 cagtgaaatg gcattttgat tattcgtggc gcttttgatt tcgctgtctg tttattttct
    49861 gacaagcagc gtaaactccg cgtcttcctc ttccagtgat cgacctgcat gcataactcc
    49921 cccgcagtct ccagcgcgaa atcgtttgac ctgacctcga cggcgttttt aatcgttgcc
    49981 tttctcaccg gtattgcggg tgctctgcaa accccgacac tcagtatttt ccttaccgat
    50041 gaagtacatg cccgtccggc gatggtggga ttcttcttta ccggcagcgc tgtcattggg
    50101 attctggtaa gtcagtttct cgctgggcgc tctgataagc gcggcgatcg caaatcgctg
    50161 attgtctttt gctgcctgtt aggcgtgctg gcctgcaccc tttttgcctg gaatcgcaac
    50221 tactttgttt tgctattcgt tggcgtcttt cttagcagct ttggctcgac cgctaacccg
    50281 caaatgtttg cccttgcccg tgaacatgcc gacaaaaccg gacgtgaggc ggtgatgttc
    50341 agctcttttt tacgcgctca ggtttcactg gcatgggtca ttggcccacc gctggcttat
    50401 gcgttagcga tgggtttcag ctttacggta atgtatctga gcgcagcggt agcgtttatt
    50461 gtttgcggcg tgatggtgtg gctgttttta ccgtcgatgc aaaaagagct tccgctggcg
    50521 accggcacgg ttgaagcgcc gcgccgtaac cgtcgcgata cgctactgct gtttgtcatt
    50581 tgtacattga tgtggggctc gaacagcctg tacatcatca acatgccgct atttattatc
    50641 aacgaactgc atcttcccga gaaactggcc gatgtgatga tggggaccgc cgccgggctg
    50701 gaaatcccga cgatgttgat tgccggatat ttcgccaaac gtctgggtaa gcgtttctta
    50761 atgcgcgttg ctgccgtggg tggcgtctgt ttttacgcag gaatgctgat ggcgcattca
    50821 cctgtcattc tgttgggctt gcagctgcta aatgctattt ttattggcat tctgggcggc
    50881 atcgggatgc tctattttca ggatctgatg cccggtcagg cgggttcagc caccacgctc
    50941 tataccaaca cttcgcgcgt gggctggatc atcgcaggat cagtggcggg catcgtcgcc
    51001 gaaatctgga attatcacgc tgtgttctgg tttgcgatgg tgatgattat cgccactctg
    51061 ttttgcttac tgcggattaa agatgtttaa ggcgcggtca gcatttccag atcgataagc
    51121 caggtcatgg cctgctgacg cgagttaccg cacatttcag cgctggcttg taagcctgca
    51181 cagactttcg gtcgcagtgg cgaggtgaaa attttacagc gttgctgttc atcaagctga
    51241 atgcagggcg tattggcggg cttgccatct ggcataccgg gaatagggct ggaaattgaa
    51301 ggggcagtgc aacacgcccc gcaacccgga cggcattcca tacgttgttc tcttaaggtt
    51361 aaccaccgga ttgatgagcg cgcacagtac cacctttttt gcaccagcaa aagtgcgaat
    51421 accacttgcc cgaaaggccc gtcgcgagta ctttgtcgcg atatttttga cattttcgac
    51481 tacaggaatt tttcgatgcc aagagcgaac gaaattaaaa aaggtatggt actgaattac
    51541 aacggcaaac tgctgctggt taaggatatt gatattcagt cgcccactgc ccgcggtgcc
    51601 gctacgctgt acaaaatgcg tttttctgat gtccgtaccg ggctgaaagt agaagagcgt
    51661 ttcaaaggtg atgatatcgt tgacaccgtg acgctgaccc gccgttacgt tgacttctcc
    51721 tatgtcgatg gcaacgaata tgtctttatg gataaagaag actatacccc gtataccttc
    51781 accaaagatc agattgaaga agagttgctg tttatgccag agggcggcat gccggacatg
    51841 caggtgctga cctgggacgg tcaactgctg gcgcttgagc tgccgcagac cgttgatctg
    51901 gaaatcgttg aaaccgcacc aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg
    51961 gcgacattga gcactggtct ggtgattcag gtaccagaat acttaagccc gggcgaaaaa
    52021 attcgtatcc atatcgaaga acgccgttat atggggcgtg ctgactaact tcagccgcat
    52081 gcagaaaagg gatagctcag gctgtccctt ttttaattta ttataccaat cttctatttt
    52141 gcgcttcctg aacaccttac gccctcaatt ttcactcgtt gattgatcgc cctcacactt
    52201 catcgcatta acaatccaga ccaatttcaa ttgctgtcat ataactttac actgtcattg
    52261 ttaatttatc gttactaaga cgtgactcct atgaagacaa ttgcctccgt tacgctcccg
    52321 catcatgtac acgctccacg ctacgatcgc cagcagttgc aatcacgtat cgttcatttt
    52381 ggctttggcg cctttcaccg cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc
    52441 cagggcggcg actgggggat ctgtgaaatc agcttgttca gcggtgatca actgatgagc
    52501 cagctccgcg cacagaacca tttatatacc gtgctggaga aaggtgcgga cggcaatcag
    52561 gtgataattg tcggtgccgt tcacgaatgc cttaatgcaa aactggattc cttagcggca
    52621 attattgaga aattttgcga gccacaggtg gcaattgttt ccctgacgat taccgaaaaa
    52681 ggctattgta ttgacccggc caccggtgca ctcgacacca gtaatccgcg gattattcac
    52741 gatctacaaa cccctgaaga acctcactcc gcaccgggta ttctcgtcga agcactgaaa
    52801 cgccgccgtg agcgcggcct tacaccgttt accgtgctct cctgcgacaa tattcccgac
    52861 aatggccatg tggtgaaaaa cgcggtgctg ggaatggcag aaaaatgttc gccagaactc
    52921 gccgggtgga taaaagaaca cgtcagtttt ccgggaacca tggtcgaccg cattgttccg
    52981 gctgcaaccg acgaatcact ggcagaaatc agccagcatc tgggggtaaa tgatccctgc
    53041 gcgattagct gcgaaccgtt tatccagtgg gtggttgaag ataacttcat cgctgggcgt
    53101 cctgcctggg aagtcgcagg tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg
    53161 aaactgcgta tgcttaatgg cagccactct tttctcgctt atctgggtta cctctcagga
    53221 ttcgtccata tcagtgattg catgcaggat cgcgtatttc gccatgctgc cagaacatta
    53281 atgctggatg agcaagcgcc gacactgcaa attaaagatg tcgatttaac acaatatgcg
    53341 gataagttaa ttgcacgttt tgctaatccg tcgctgaaac ataagacctg gcaaatcgcg
    53401 atggatggca gccagaaatt accgcaacgt atgctggcag gtattcgcat acatctgggg
    53461 cgtgaaacgg actggtcgtt gctggcatta ggcgttgctg gctggatgcg ttacgtcagc
    53521 ggcgttgatg atgccggaaa tgccattgat gttcgcgatc cgcttagcga taaaattcgc
    53581 gaacttgttg cgggcagcag cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa
    53641 gttttcggtg atgatctgcc agataacccg cattttgtgc aggccatcga acaagcctgg
    53701 caacaaatcg tacaattcgg cgcacatcag gcgctattaa acaccctcaa aatttaacga
    53761 tttctgcggt taaagcggat gaagctcacc ttcgtccgct ctccccttct cttttctgcc
    53821 ttttttagcc aggattaacg ctcagttaac ttaccagagt attgcggagc cgttgtgacc
    53881 aggaccaacc tcatcaccgg ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg
    53941 ttagcccata aagatcccaa cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc
    54001 ggaattgatg gtgctttgct cgccgatagc ggcgcattgc tgaaagagat ccccggcggc
    54061 tgcatgtgct gcgttaatgg tttacccatg caggtagggt tgaatacctt actgcgtcag
    54121 ggaaaaccag accgcttgtt gatagagccg accgggctgg gccatccgaa acagatcctc
    54181 gatctgttaa ccgcaccagt ctatgaactg tggatagatc tgcgcgccac cttgtgcatt
    54241 ctcgatccac gcctgctgct ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag
    54301 ctggctgccg cagacatcat tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag
    54361 caagcgctac agcgttggtg gcagcaaaat ggtggcgatc gacaattaat tcacagtgag
    54421 catgggaaag ttgacggtca tcttctggat ttgccgcgtc gcaatttagc cgagttgccc
    54481 gccagcgccg cgcattctca tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg
    54541 ccagagcatc aacgctggcg tcgcagtctg aacagcgggc aaggatatca ggcctgcggc
    54601 tggatattcg acgctgatac ggtattcgac accattggca ttctggaatg ggcgcgactt
    54661 gcaccggtgg aacgcgtcaa aggcgtgctg cgtattcccg aagggctggt acgaatcaac
    54721 cgtcagggcg atgacctgca cattgaaacg caaaacgttg cgccaccgga cagccgtatt
    54781 gagctgattt ccagcagcga agctgactgg aatgctttgc agagcgcgct gttgaagctt
    54841 cgtttagcga ctaccgcgta aggttgcctg cgtttttcag taagataatt agagaaaata
    54901 tgattaaaaa tttgccgcaa atagtgttgt tgaatattgt cggcctcgcg ctgtttcttt
    54961 cctggtatat tcccgttaat catggattct ggttgccgat tgatgcggat attttttatt
    55021 tctttaatca gaaactggtc gaaagtaagg cctttttgtg gctggttgca ttgaccaaca
    55081 atcgcgcttt cgacggttgt tctctgctgg cgatgggaat gttgatgctg agtttctggc
    55141 tgaaagaaaa cgcccctggc agacgacgta tcgtgattat gggtctggtc atgctattaa
    55201 ctgcagtggt attaaaccag ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa
    55261 cattgacttt taccgatatt aaccgcgtca gcgaactgct ctctgttccc acgaaagatg
    55321 cctcacgaga tagctttccc ggcgatcacg gcatgatgct gcttattttt tcggcattca
    55381 tgtggcgtta tttcggcaaa gttgcaggcc ttatcgccct tattattttt gtggtttttg
    55441 catttcccag agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga
    55501 ccgtgatatt gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca
    55561 ccttttttga caaatcacta ccgggaaaaa acaaacattt ccaaaacaaa taactcacag
    55621 taattaacat catcaggctt atttttatag tgaggataat cctgatgatg cgcaccgtgc
    55681 tttcatccat cgcgcgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca
    55741 ctcaatcgta ctttaattga ccaatcccgc ttattaactt tctgtatcac tttttcttat
    55801 aaaaaatcat gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt
    55861 tgcctcggcg gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg
    55921 ccttaaggaa ttgtttcaac atgcccaggt aattagtctc gtgccgcttg gcattttttt
    55981 ataacgatat ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac
    56041 cgattttgag atatatcttg cgcgggatcc ccgcgattgc agtagcggtt ctgctttctg
    56101 catgtagtgc aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg
    56161 aaacatcatc actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg
    56221 taaaatcgcg aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg
    56281 gcagcactaa aaaaggtatc gattgttctg gttttgtaca acgtacattc cgtgagcaat
    56341 ttggcttaga acttccgcgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc
    56401 gcagtaattt gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg
    56461 tcggtattta tatcggcaac aatcagtttg tccatgcttc caccagcagt ggtgttatta
    56521 tttccagcat gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca
    56581 gccgcagcta ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa
    56641 gcactgctta ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc
    56701 ttgatccagg ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa
    56761 ttaacggaat catgttcata cgcgctccca attctggacg taagctcctg cttacctgca
    56821 ttgtagcagg cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc
    56881 ataagcacga agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct
    56941 attttgccga cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc
    57001 agcaggctaa ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg
    57061 tgctggtgaa agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac
    57121 tcaatgaatt gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg
    57181 gcacgccgat ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga
    57241 aaaatagcgg tgtgtttgcc gctctgaatc tcaacctgac gccttctctc ttttatagtt
    57301 cacggcagga agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct
    57361 tttcttcacg tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa
    57421 ttgcgggcat tcctctgacc gttcggcttt atgcggatga ctggacatgg aacgatgtgt
    57481 ggtacgcatt tttactgggc ggcatgagtg gaactgtcgt tggcctgctc tgctattacc
    57541 tgatgagcgt acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat
    57601 tttacgtggc gtatcaaccg gtggtggata cacaagcttt gcgagtaacg ggcctggaag
    57661 tactgctacg ctggcggcat cctgtcgcgg gagaaattcc cccggatgcc ttcattaact
    57721 ttgccgaatc gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc
    57781 gcgatgccgc agaattagaa aaagtgctgc cagtaggcgt caaatttggt attaacattg
    57841 cgccggacca tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc
    57901 tgcccgcaca ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc
    57961 aagaagccac acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg
    58021 acttcggcac cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga
    58081 aaattgaccg tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg
    58141 acgcggtgct gacgctggcg aaacgcctca atatgctgac ggttgctgaa ggggtcgaaa
    58201 cgccggaaca ggcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga
    58261 ttagtcgccc tttaccgctg gacgattttg tccgctggct gaagaaaccg tatacgccgc
    58321 agtggtaagg tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct
    58381 aatgatgagt aaaaggaaat ccattgcaga tgattgtgcg catactgctg ctgtttatcg
    58441 ctctgttcac ctttggtgtg caggcgcagg ctatcaagga aagctatgcc tttgccgtgc
    58501 tgggcgaacc ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc
    58561 caaaaggtgg gcagataacg ttgtcagccc tcggcacctt cgataatttc aaccgctatg
    58621 cactgcgcgg caacccgggc gcacgcaccg agcagctgta cgacacgcta tttacgactt
    58681 ccgatgacga accaggcagt tattacccgc tgattgctga aagcgcacgc tatgctgacg
    58741 attattcctg ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttcgccca
    58801 ttactgcccg cgatgtagag tttacttttc aaaaatttat gaccgaaggc gtgccgcaat
    58861 ttcgtctggt ctacaaaggc accaccgtca aagccattgc accgttaacc gtgcgcattg
    58921 agttagctaa acccagcaaa gaagatatgc tgagtctgtt ttcgctgccg atatttccag
    58981 aaaagtactg gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg
    59041 gtccgtaccg cgttacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag
    59101 attactgggc agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct
    59161 acgattatta cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt
    59221 tgcgtatgga aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata
    59281 aaaaatacat catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg
    59341 cgtttaatat ccaacgtccg gtattcagcg atcgccgggt ccgggaagca atcactctcg
    59401 cctttgactt tgaatggatg aacaaggcgt tgttttacaa cgcctggagt cgcacgaaca
    59461 gttattttca gaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc
    59521 ttctggcacc aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc
    59581 cggtatctaa aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca
    59641 acgaaacggg ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccgc
    59701 tcagctttga attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc
    59761 acagcctgca acggctgggt atcaatatgg acattcgcaa ggtggataac tcgcaaatca
    59821 ctaaccgcat gcgcagtcgc gactatgaca tgatgccgcg cctatggcgg gcgatgccgt
    59881 ggcccagttc cgatttacag atttcctggt catcggaata tatcaattcc acttataatg
    59941 cccccggcgt gcaaagcccg gttatcgact cgctgatcaa ccaaattatt gccgcgcagg
    60001 gaaataaaga aaaattactg ccgttggggc gagcactgga tcgcgtatta acgtggaatt
    60061 attacatgct gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct
    60121 cccaacccgc tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta
    60181 ataaagcggc caaactgccg tcagccagac aacagggaga gtagatgggc gcttacctga
    60241 ttcgccgtct gttgctggtg atcccaacgt tatgggctat tatcactatc aactttttca
    60301 tcgtgcaaat tgcgcctggc ggtccggtcg atcaggccat cgccgccatt gagtttggta
    60361 atgccggagt attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg
    60421 gtgtcggcaa tatcagcgac agtaattacc gtggcggacg cggattagac ccagaagtga
    60481 tcgctgagat cactcatcgc tacggtttcg ataagccgat ccacgaacgt tacttcaaaa
    60541 tgctctggga ctacatccgc tttgattttg gtgatagcct gtttcgcagc gcctcggtgc
    60601 tgacgctgat taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga
    60661 ttatctatct ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagtcgct
    60721 ttgacgtctg gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg
    60781 ccatcctgct gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg
    60841 gcctggtttc cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt
    60901 ggcatatcac gctaccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc
    60961 tgaccaaaaa ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga
    61021 aaggggtaag tgagaaaaac attctctgga aacatgtgtt ccgcaacgcc atgctgctgg
    61081 tgattgccgg ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg
    61141 aggtgatgtt ttcactcaat ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg
    61201 attatcctgt aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata
    61261 ttgtcagtga tatcagctat acgctggttg atccgcgtat agattttgag ggacgttaat
    61321 gtcgcgactc agccccgtca atcaggcccg ttgggcgcgt tttcgtcata accgtcgcgg
    61381 ctactggtcg ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat
    61441 cgctaacgat aaaccgttgc tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa
    61501 aaactacagc gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc
    61561 gtggctgaaa caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg
    61621 tgctaccagt atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa
    61681 ctggctggga acggatgcca acggcggcga tgtgctggca cgcattctct atggcacgcg
    61741 gatctcggtt ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc
    61801 gggggcgcta caaggctatt acggcggtaa agtcgatctc tggggacaac gctttattga
    61861 agtatggtcg gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc
    61921 taacttctgg tggctgctgg caattactgt cttgtttggc tggatgagtc tggtcggcgt
    61981 ggtgcgggcg gagtttttac gtactcgtaa tttcgactac attcgtgcgg cacaggcgct
    62041 tggcgtcagc gatcgcagta tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac
    62101 cctcaccttt ttaccgttta ttttatgtag ttcgataacc accctgacct cgctcgattt
    62161 cctcggcttc ggtctaccgc tcggttcacc gtcactcggt gaactgctgt tacaagggaa
    62221 aaataacctt caggccccgt ggcttgggat caccgccttc ttgtcggtgg cgatattatt
    62281 gtctttgctg atctttattg gtgaagccgt ccgcgacgca tttgatccta ataaggcggt
    62341 gtagcatgac gcaaactctg ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc
    62401 aaaccgtacg tacagtagtc aatgatgttt cactacagat tgaggctggc gaaacgctgg
    62461 cgctggtggg tgagtcaggt tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc
    62521 tcccttcccc gccggttgaa tatctctccg gcgatattcg ttttcatggc gaatcgctgc
    62581 ttcatgccag cgatcaaacg ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc
    62641 aggaaccgat ggtgtcgtta aatccattgc ataccctgga aaaacagctt tatgaagtgc
    62701 tttcactcca ccgcgggatg cgtcgggaag cggctcgtgg cgaaattctt aactgccttg
    62761 atcgcgtcgg tatccgccag gcggcaaaac ggctgacaga ttatccgcat cagctctccg
    62821 gcggcgaacg ccagcgggtg atgattgcga tggcgctgtt aacgcgaccg gaattattaa
    62881 ttgccgatga accgaccacc gcactggacg tctctgtcca ggcgcagatt ttacagctgt
    62941 tgcgcgaact gcaaggcgag ctgaatatgg gcatgctgtt tattactcat aacctcagca
    63001 ttgtcagaaa actggcccac cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa
    63061 ataacgccgc tacgctattt gcctcaccca ctcatcctta cacacaaaag ctactcaaca
    63121 gtgaaccgtc aggcgatccg gtaccgttgc cagaacctgc ctcaacgttg ctggatgttg
    63181 aacagcttca ggttgccttc cccattcgca aagggatttt gaagcgcatt gtggatcata
    63241 atgtggtggt gaaaaacatc agttttacgc tacgggcggg tgaaacactg ggtttagtgg
    63301 gcgagtctgg ttccgggaaa agcacgacgg gactggcgct gctgcgactg attacctctc
    63361 agggcagcat cgtctttgac ggtcagccac tgcaagattt aaatcgccgc cagctgttac
    63421 ctactcgtca tcgtattcag gtggtatttc aggatccaaa ctcctcgctc aacccacgac
    63481 tcaacgtttt gcagattatt gaggaaggct tacgggttca ccagccgacg ctttctgccg
    63541 cacaacgcga acaacaagtg atagccgtga tgcatgaagt gggattagat cctgaaacac
    63601 gccaccgtta tccggcggag ttctctggtg gtcagcgaca acgtattgcg attgccaggg
    63661 cattaattct taagccctca ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa
    63721 cggtacaggc gcaaatattg acgctattga aatcattgca acaaaagcat caactggcct
    63781 atttgtttat cagccacgat ttgcacgttg tccacgcgtt atgtcatcag gttatcgtac
    63841 tgcgacaagg ggaagtagtg gaacaaggac cgtgcgcgcg cgtgtttgcc gcaccgcagc
    63901 aggagtatac gcgtcagcta ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa
    63961 aagggttcgg caattgccac accaaaattt ttcaatcggc atgttgcagc aaactcgtcc
    64021 tggcgattta caaacaggca cggctcacct tcacactcca gcaccgaaca tggaacttcg
    64081 atgtcgctta acgcctggct tagtttatac atcaccgacc atgcattatc accatccggg
    64141 ctaagcaatt taagcgccac caggctgttg tcaccgcacg gtccattttg cggaatcggt
    64201 tcaaccttcg aacctgcgaa accagcagac cagcgaaagt tctgcggtag acgatggacg
    64261 attgagagtt gtaatgaagt cacttatttt ccccggaagc acatttactt cacaatttat
    64321 ttacatcaat tttaacacat catcaacaat ccgtctttat acagatcgta aagattgatg
    64381 cctcgcctgc atggcctcta tggctctgat ttaagtaata tctgcgggcg tggtcacgtt
    64441 tgcggggcta tatgtacccg tttctgcgat ctaactcaac ctttttaact acaatgatgt
    64501 gactttttac ataaattgat tttacataaa ataaacatat atcgggggaa tgatagattt
    64561 gtggttgaca tgcatcaaca aaggaagcct tttagcttcc tcgttgtgca atagatcacc
    64621 gttttttcgg tcgactggcg tacagacaga agagaatgga gctggttgcg cagaatgcaa
    64681 ttgaccaaat catcggccat gcagagttaa aggtcgcaag agaaagcaat gcgccaacaa
    64741 ttgcccctat gccaaaacgg aaggttcctg ccagcgaaga tgccgttccc gccatatggg
    64801 gaaactcatc aagaatgacc gccatcgcat tggatgacac catagacacg cagcccacaa
    64861 acgccgcaac gccaaccacc agcgaccaga atcccagccc cagcagcgca ctgatgacca
    64921 tccacgctgc cataataaat tgtatccaca accccgagcg gaacatattt aacgcgccaa
    64981 tgcggcggac gaagcggctg ttaaagatgg tcatcacgaa cagaaaaaca atgtttagcg
    65041 caaagtaata accaaagttt tccggtgcaa cgtggttgat ttcaatgtag acaaacggtc
    65101 cggcgctcag gaacgagaac attccggcaa agctgaaacc gctggccagc atataactca
    65161 ggacacgctt atgacggaac agcgccgcaa agttaccaat agtggtacga atgtgaaaag
    65221 gctggcgagg atccagaaga tgtaatgcca gctcagccac accagcaccc agccgccaac
    65281 tatcggtgcc atcagcggtg caatggtcgt caccagcatg acaaacgaca tcatccgcga
    65341 gaactcttct ttcgggtaaa tatcacgcat caaagcgttg ataaccacgc tggccgcagc
    65401 cgcagccagc ccgtggaaga aacgcatcac aatcagctga tcgatggttt gtgccaacgc
    65461 acacgccacc gcggcggcgg caaacaccag cgtaccgcca agaaccaccg gcttacgccc
    65521 gaagctgtct gccatcggcc cgtagattaa ctgccccaac gcaaagccca gaatataagt
    65581 actcagggtc atctgcgtac tgcccgccgg tacgccaaac tgcgctgaaa ttaccggtag
    65641 cgcgggcaga tacatatcaa tcgacagcgg catcaacatg gccagcaggc caaggataaa
    65701 aacaatagca aacgacgaat gctgtcgggt ggtcacaacg ggctcctgaa agtcattgaa
    65761 aagttagacg acgctggcaa tttcttcttc agttaacgga cgatattcac cgggggctaa
    65821 atcagcatcc agcgtaatgc cgccaatacg ttcacgatgc agctcaacca catggttacc
    65881 cacggcggcg aacatgcgtt tcacctgatg ataacgccct tcgctgatgg tcagacgaac
    65941 ctgcgttggg gtaatcactt ccagcaccgc cggcttagtg agatcttttt cgttatgcag
    66001 ctgcacgcct ttagcaaatt gttctgccgt atcgtcagct acgggtgata ccagcgtcac
    66061 cagataggtc ttctcgcaat gatggcgcgg agaagtaatg cggtgcgacc actgaccatc
    66121 atctgtcatt agtaccagac cggtggtatc gatatccagc cgccctgcgg cgtgcagttt
    66181 ccacgctacc ggttcatcta gaaaatagag caccgttggg tgatcagggt cgtccgtgga
    66241 gcaaacataa ccctgaggct tattgagcat gaagtaacgt ggaccgtgtt gctgcgccag
    66301 cgggttgcca tcgtaagcga catcatgttc aggaagcagt ttgaacgctg cattacggac
    66361 gatttcgcca tcgacggtga cacgattgcc gcggatttca cgcccggcaa tagcacggct
    66421 aacgccgagt tgctgtgcga taaatttatc aagtcgcatg tgtgtgattt tgcctgtaaa
    66481 aaacggaggt cgggtaatga cccgaatatc tgaactgttg tctgcccagt atagcggtct
    66541 aataacatcc ctcaagggaa aaagatccgt ggcatactat tagcagaata atctacctac
    66601 gcgagaccat gatttttaca cttcgcccat atcagcaaga agccgtggat gccacgctca
    66661 accattttcg tcgtcataaa acccctgccg ttatcgtgct gcccaccggt gcaggtaaaa
    66721 gcctggtgat agcggaactg gcacggctgg ctcgtggtcg cgtactggtg ctggcacacg
    66781 ttaaagaact ggtggcgcaa aaccatgcaa agtatcaggc gctggggctg gaagccgata
    66841 tttttgccgc cgggctgaag cgtaaagaga gccacggtaa agtggtattt ggcagtgtgc
    66901 agtcggtcgc ccgtaatatt gatgcctttc agggtgaatt ttcgctgttg attgtcgatg
    66961 aatgtcaccg tattggtgac gatgaagaga gccagtatca gcaaatcctc actcacctga
    67021 caaaagtgaa tccccactta cgcctgctgg ggctgactgc cacgcctttt cgattgggca
    67081 aaggttggat ctaccagttc cattatcacg gcatggtacg cagcgatgag aaagcccttt
    67141 tccgtgactg catttatgag ctgcccctgc gttatatgat taaacacagc tatctgacgc
    67201 cgccagaacg actggatatg ccagtagtgc aatatgattt cagccgcttg caggcacaga
    67261 gcaacgggct gttcagcgaa gccgatctca accgtgagct gaaaaaacag caacgtatta
    67321 ccccgcacat catcagccag attatggagt tcgccgaaaa gcgtaaagga gtgatgattt
    67381 tcgccgcgac ggtcgaacac gcaaaagaga ttgtgggatt actgcctgcc gaagatgcag
    67441 cactgattac tggcgacacc cccggcgctg agcgcgatgt gttaattgaa aattttaaag
    67501 cccagcgttt tcgctatctg gtcaacgtcg cggtactgac caccggattt gacgccccgc
    67561 acgtcgatct tatcgccatt ctgcgcccta ccgaatcagt gagtctttac caacaaattg
    67621 tcgggcgcgg tctgcgtctc gctccgggca agactgattg tttaattctt gattatgcgg
    67681 gtaatcctca cgatctctac gcgccggaag ttggtacacc gaaaggcaaa agtgacaacg
    67741 ttccggtaca ggttttctgc cctgcctgcg gttttgccaa caccttttgg gggaaaacga
    67801 ccgccgacgg gacattgatt gaacactttg gtcgccgctg tcagggatgg tttgaagatg
    67861 acgacggtca tcgcgaacag tgtgacttcc gtttccgttt taaaaattgc ccgcaatgta
    67921 acgcagaaaa tgatattgcc gctcgccgct gccgcgaatg tgacacggta ctggttgatc
    67981 cggatgatat gttaaaagcg gcgctacgac tgaaagatgc gctggtatta cgctgtagcg
    68041 gcatgtcttt gcagcatggg cacgacgaga aaggcgaatg gttgaaaatc acctattacg
    68101 atgaagacgg tgcggatgtg agtgagcgtt tccgtctgca aacacctgcc cagcgtaccg
    68161 ccttcgagca gctttttatc cgcccgcata cgcgcacacc gggcatcccg ctgcgctgga
    68221 tcaccgccgc cgatatcctc gcccagcaag ccttattgcg acccccggat tttgtcgtcg
    68281 cccgcatgaa aggccagtac tggcaggtgc gtgaaaaagt gttcgattac gaaggtcgtt
    68341 ttcgtcgggc gcacgaatta cgcggttaat ggtagttttg attgatgtat aaggcgattg
    68401 agtatagaat ctcacccgct tttgcatacg caaagcagat cacttacctg ttgctgggtc
    68461 gcctgtagca ggattaattt aagagagaaa gaaatgttta ctatcaacgc agaagtacgt
    68521 aaagagcagg gtaagggtgc gagccgccgc ctgcgtgccg ctaacaagtt cccggcaatc
    68581 atctacggtg gcaaagaagc gccgctggct atcgagctgg atcacgacaa agtcatgaac
    68641 atgcaagcta aagctgaatt ctacagcgaa gttctgacca tcgttgttga cggtaaagaa
    68701 atcaaagtta aagctcagga cgtacagcgt cacccgtaca aaccgaagct gcagcacatc
    68761 gacttcgttc gcgcttaatt gctgaataag ttgtaaaaaa ccccgctccg gcggggtttt
    68821 ttgtatctgc agattatgcc tgattacggt attgctattt tttgcaggcc agataaggcg
    68881 ttcacgccgc atctggcatt cagaacacaa aaccgattaa ttgccgccag acgtccggcg
    68941 ctgcaattgg tcgcgcaaat tcggcggcgt gcctttaatg gtcagggtat cggtcgccgg
    69001 ctcccagaag atgcgttcac ctaacagcat cgcatcaaag ttaatagtca agccaccgcc
    69061 gctaccggca aacttcgtca actgacgcag tgtgctccgg tctgccggga agctctcttc
    69121 cagttcgtag cctttttcag ccgcaaactc cgtgaagctc acttcgctta cgcctgccag
    69181 ctctttcgac agcgattcca gttcgatctc ttcgcctgcc tgcagctgct cattacagta
    69241 gctataaacc tgctggcgca cgttctgtcg ctctgcttta tccagttgtg cttcggcagt
    69301 gaaatcatca accgcttgca acagcccacg gttttgcgct ttcgtgttca atccttcgct
    69361 ggccccgagg aaatccataa agaaatccgc cactttgcgc cctactcgcc ctttcaggaa
    69421 agtgagatag cgggtggact ctggattggt ttcccattcg gttaaatcga tacgcgcaac
    69481 aatatccgca tgattgatat caagataatg ggttgggttg atgtcgagat tttcgttaac
    69541 acgcatactg ctcaggttgc tcagaactgc caccagcaga tactccaccg ccagataacg
    69601 gtagtggcaa aacagcacga atccaccgtc agcaaaagga tatttcgcta attcgtcacg
    69661 caggcgacca gttgccgcgc ggctaaatgc cagaaagtcc tcttcgccct gacgctgtaa
    69721 gcgcagcgtc tgcgccagtt cactctcttc gctaaacaag ccgtaagctt tatttttggc
    69781 gctatagacc cgatgcagtt ctgccaccat ctcaacgacg gtttctgtcg gttccagcaa
    69841 tgaatcgcgc aacaccagct caaggttctg ctcatcacgc ttgataagct ggtgcagagc
    69901 aatctggttg atatccagac tcatgataaa ctctccttta agaccgggcg gtattcaacc
    69961 accgccggtt aagcgacgca ataaaagatg gcggtcattt tacagaaggc agcgtaacga
    70021 gaatgattta acgcataaaa aagcagaaaa aaaaccgttg ctacggtaat atgttgccct
    70081 ttcatcaact aactgatttc gatttatgcc acaaatttcc cgctacagtg atgaacaggt
    70141 tgaacaattg ctcgcagagc tgctcaacgt actggaaaaa cataaggctc cgaccgacct
    70201 ttccctgatg gtgttaggca acatggtcac taaccttatc aacaccagca ttgccccggc
    70261 ccaacgccag gcaattgcca actcttttgc ccgcgcctta cagtcctcta tcaacgaaga
    70321 caaagcgcac taagggaaac agataacagg ttatggtaac tcatcgtcag cgctaccgtg
    70381 aaaaagtctc ccagatggtc agttgggggc actggtttgc actgttcaat attctgcttt
    70441 cgctcgtcat tggcagccgt tacctgttta tcgccgactg gccgacaacg cttgctggtc
    70501 gcatttattc ctacgtaagc attatcggcc atttcagctt cctggtgttc gccacctact
    70561 tgctgatcct cttcccgctg acctttatcg tcggctccca gaggctgatg aggtttttgt
    70621 ccgtcattct ggcaacggcg ggaatgacgc tattactgat cgatagcgaa gtctttactc
    70681 gtttccatct ccatcttaat cccatcgtct ggcagttggt tatcaaccca gacgaaaatg
    70741 agatggcgcg cgactggcag ctgatgttca tcagcgtgcc ggttatttta ttgcttgaac
    70801 tggtgtttgc gacgtggagc tggcaaaagc tgcgcagcct gacgcgtcgt cgacgcttcg
    70861 cgcgcccgct ggccgcattc ttatttatcg cctttatcgc ctcgcatgtg gtgtatatct
    70921 gggccgatgc caacttctat cgcccgatca ccatgcagcg cgctaacctg ccgctttcgt
    70981 acccgatgac ggcgcgacgt tttcttgaga agcatggtct gcttgatgcg caggagtatc
    71041 aacgccgtct tattgagcaa ggtaatccag acgccgtttc cgttcagtat ccgttaagcg
    71101 aactgcgcta tcgcgatatg ggcaccgggc agaatgtgct gttgattact gtcgatggcc
    71161 tgaactactc acgcttcgag aagcagatgc ctgcgctggc aggttttgct gagcaaaata
    71221 tttcgttcac gcgccatatg agctccggca acactacaga caacggcatc tttggcctgt
    71281 tctatggtat ctcgccgagc tatatggacg gcattctgtc gacccgtacg cctgcggcat
    71341 taattactgc gcttaatcag caaggctatc agctggggtt attctcatca gatggcttta
    71401 ccagcccgct gtatcgccag gcattgttgt cagatttctc gatgccgagc gtacgcaccc
    71461 aatccgacga gcagaccgcc acgcagtgga tcaactggct gggccactac gcacaagaag
    71521 ataaccgctg gttctcgtgg gtttctttca atggcactaa cattgacgac agcaatcagc
    71581 aggcatttgc acggaaatat agccgggcgg caggcaatgt cgatgaccag atcaaccgcg
    71641 tgctcaatgc actgcgtgat tctggcaaac tggacaatac ggttgtgatt atcactgccg
    71701 gtcggggtat tccgctgagc gaagacgaag aaacctttga ctggtcccac ggtcatctgc
    71761 aggtgccatt agtgattcac tggccaggca cgccggcgca gcgtattaat gcgctgactg
    71821 atcataccga tctgatgacg acgctgatgc aacgcctgct acatgtcagc acacctgcca
    71881 gcgaatattc gcaaggtcag gatttgttca accctcaacg ccgtcattac tgggttactg
    71941 cagcggataa cgatacgctg gcaattacca ccccgaaaaa gacgctggtg ctgaacaata
    72001 acggtaaata ccgcacttac aacttacgtg gtgaaagagt gaaagatgaa aaaccacagt
    72061 taagtttgtt attgcaagtg ctgacagacg agaagcgttt tatcgctaac tgattaatta
    72121 taaatcagtt agcgaaatat cttacttgca atcggtgtgg aaaacggtag tattagcagc
    72181 cacgagtcgg cacgtagcgc agcctggtag cgcaccgtca tggggtgtcg ggggtcggag
    72241 gttcaaatcc tctcgtgccg accaaaaatc ccaagaaaaa accaaccttt acggttggtt
    72301 tttttatatc tgcaattaat ttgataaaca gaccgtgaca catcacagcc tgtttatttt
    72361 ctgttatcag aacgtccaga ccactcccgc ctgagtattc cacggcgatt ctacccctgc
    72421 gccattgcca tagctgactg acaaatgacc gcttaacgtc ggggtaaacg atgaccttat
    72481 cccagcctga taaacgccac gggttccagc cacatcatta ttaaatttgc catcgtcatt
    72541 aactttcaca tggttagaat cggcctattc ctgacgcacg gcggctttca gccagggttc
    72601 caacgtcgta ccgttttgca ggtccatgtg atagcttacc gccgttcccg cttcagcgcg
    72661 taatatccgg gtatttccca catccgcgcg catgccgttt gataacgtgt agtcctgacc
    72721 atctgtggta aagccggtaa aggcgagata ggggctaaca ctccacaatc cgtcaaccca
    72781 acggaacccg ctctcaacat gagcacccgc gccgttacta ttgtaatcgc caaacgctgt
    72841 tgccccatta ctcatcttgc catggatggt gttggcaaaa cggtcaactt tcaccacccc
    72901 atcaacatag gcaccgttct gatgctccca accggcataa gcccccaggg tgtagctatc
    72961 aatattgcct ttgccgccgc gatcaaaacc aatatcagaa tgagagtaat caaagaacaa
    73021 gccgcgaatt gtgctgcttt cttcacggga gaaacggcta tcgataccga gcgtcaggcc
    73081 cgtcaatgtt tgctcaaagc cagctcccgc atcagtggtc acgttgttgc gggtgttaat
    73141 tgccgaactc cacatcgccg tatcgtaatt aacgcctttt acgctaccaa gacgctcacg
    73201 cacggtgtcc agttctgcat caaataccag cggttgtgct gctgccatgt tcagcacatc
    73261 agtggttgaa ggggtaattt gcgcgcgatt ctctgcgagg ctccagctat ggttgccatt
    73321 atccagcaag gtatattcat acgtaccgat atcaacaacg cctccggcat tgcccaacgt
    73381 gaatgcagca tcaccgccgc ccgtcgttac cagtgtaagg ctatctcctg ctgccgggct
    73441 ggcccccgtg tccgtcacga atattttgaa atcacctgtt gcctgaccga tgacgttgag
    73501 ctgatcgctc tgatgattag ccatatcggt acgcatataa aattgccgtt tcccgacagg
    73561 gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga acgagtcact
    73621 ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa gttgaggcga
    73681 gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt ttcgagcgaa
    73741 acgtccgctt ggctggtatc cacggcacca tgcgttctaa aagaagcgcc ctcagaaacc
    73801 actaccttac ccaccaacat agcgggtgca taatttattt tcggcttcat gacctcaaga
    73861 atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac attagcgcgg
    73921 ccattgttga taaccatatt ttcagagatg gctttagagg aatacgtgat acttccattc
    73981 tgatctgatc gaccgagatc atatacagca tttgcctgca ctttcgtacc agtatccttt
    74041 cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg agtgtcctcc
    74101 ataacaatga gcccggagcc atcatccagt tcataatttt ttgacacacc atcctttata
    74161 ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga gacaattaat
    74221 ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac ctccagtaag
    74281 ccgccactat ccacagtggt attataagcg aagtcatttt cttcaactcg caaacttccg
    74341 ccgttttcca acagcatatt gttagcaata ccattcttga tttcgaactg accgagacgg
    74401 tttgttccga atacctccat gacccgcgtg gttgttttaa tagcaccgcc tgctttctga
    74461 tctaccgcaa atgctaatcc cccgtcttta acctccagta atcctccctt ttcgatagcg
    74521 gcaaagacgg caatcccatc tttttcaaca attaagcgcc cgcctttaat cgtgggatca
    74581 ttcgccgcg
//
LOCUS       DAZPEP010000003        73971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZPEP010000003 DAZPEP010000000
VERSION     DAZPEP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 73971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.003"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(51..1301)
                     /gene="icd"
                     /locus_tag="ACHUT9_000147"
     CDS             complement(51..1301)
                     /gene="icd"
                     /locus_tag="ACHUT9_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFR1346728.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1473..2126
                     /gene="rluE"
                     /locus_tag="ACHUT9_000148"
     CDS             1473..2126
                     /gene="rluE"
                     /locus_tag="ACHUT9_000148"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFR1346729.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2136..2597
                     /gene="nudJ"
                     /locus_tag="ACHUT9_000149"
     CDS             2136..2597
                     /gene="nudJ"
                     /locus_tag="ACHUT9_000149"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFR1346730.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2651..3757
                     /gene="mnmA"
                     /locus_tag="ACHUT9_000150"
                     /gene_synonym="trmU"
     CDS             2651..3757
                     /gene="mnmA"
                     /locus_tag="ACHUT9_000150"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFR1346731.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3793..4434
                     /gene="hflD"
                     /locus_tag="ACHUT9_000151"
     CDS             3793..4434
                     /gene="hflD"
                     /locus_tag="ACHUT9_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFR1346732.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4438..5808
                     /gene="purB"
                     /locus_tag="ACHUT9_000152"
     CDS             4438..5808
                     /gene="purB"
                     /locus_tag="ACHUT9_000152"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFR1346733.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5976..6647
                     /gene="phoP"
                     /locus_tag="ACHUT9_000153"
     CDS             5976..6647
                     /gene="phoP"
                     /locus_tag="ACHUT9_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFR1346734.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6647..8107
                     /gene="phoQ"
                     /locus_tag="ACHUT9_000154"
     CDS             6647..8107
                     /gene="phoQ"
                     /locus_tag="ACHUT9_000154"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFR1346735.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8183..9304
                     /gene="roxA"
                     /locus_tag="ACHUT9_000155"
                     /gene_synonym="ycfD"
     CDS             8183..9304
                     /gene="roxA"
                     /locus_tag="ACHUT9_000155"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFR1346736.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9353..10579)
                     /gene="pepT"
                     /locus_tag="ACHUT9_000156"
     CDS             complement(9353..10579)
                     /gene="pepT"
                     /locus_tag="ACHUT9_000156"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFR1346737.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRVAELTAQRK"
     gene            10829..11965
                     /gene="potA"
                     /locus_tag="ACHUT9_000157"
     CDS             10829..11965
                     /gene="potA"
                     /locus_tag="ACHUT9_000157"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFR1346738.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11949..12806
                     /gene="potB"
                     /locus_tag="ACHUT9_000158"
     CDS             11949..12806
                     /gene="potB"
                     /locus_tag="ACHUT9_000158"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFR1346739.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12803..13597
                     /gene="potC"
                     /locus_tag="ACHUT9_000159"
     CDS             12803..13597
                     /gene="potC"
                     /locus_tag="ACHUT9_000159"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFR1346740.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13594..14640
                     /gene="potD"
                     /locus_tag="ACHUT9_000160"
     CDS             13594..14640
                     /gene="potD"
                     /locus_tag="ACHUT9_000160"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFR1346741.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14796..15617)
                     /gene="cobB"
                     /locus_tag="ACHUT9_000161"
     CDS             complement(14796..15617)
                     /gene="cobB"
                     /locus_tag="ACHUT9_000161"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFR1346742.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15633..16544)
                     /gene="nagK"
                     /locus_tag="ACHUT9_000162"
     CDS             complement(15633..16544)
                     /gene="nagK"
                     /locus_tag="ACHUT9_000162"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFR1346743.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16573..17817)
                     /gene="lolE"
                     /locus_tag="ACHUT9_000163"
     CDS             complement(16573..17817)
                     /gene="lolE"
                     /locus_tag="ACHUT9_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFR1346744.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17817..18518)
                     /gene="lolD"
                     /locus_tag="ACHUT9_000164"
     CDS             complement(17817..18518)
                     /gene="lolD"
                     /locus_tag="ACHUT9_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFR1346745.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18511..19710)
                     /gene="lolC"
                     /locus_tag="ACHUT9_000165"
     CDS             complement(18511..19710)
                     /gene="lolC"
                     /locus_tag="ACHUT9_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFR1346746.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            19972..21045
                     /locus_tag="ACHUT9_000166"
     CDS             19972..21045
                     /locus_tag="ACHUT9_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFR1346747.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21173..24619
                     /gene="mfd"
                     /locus_tag="ACHUT9_000167"
     CDS             21173..24619
                     /gene="mfd"
                     /locus_tag="ACHUT9_000167"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFR1346748.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24763..25719
                     /gene="ldtC"
                     /locus_tag="ACHUT9_000168"
                     /pseudo
     CDS             24763..25719
                     /gene="ldtC"
                     /locus_tag="ACHUT9_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25802..26059)
                     /gene="bhsA"
                     /locus_tag="ACHUT9_000169"
                     /gene_synonym="ycfR"
     CDS             complement(25802..26059)
                     /gene="bhsA"
                     /locus_tag="ACHUT9_000169"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFR1346749.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26300..26932
                     /gene="comR"
                     /locus_tag="ACHUT9_000170"
     CDS             26300..26932
                     /gene="comR"
                     /locus_tag="ACHUT9_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFR1346750.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(26994..27533)
                     /locus_tag="ACHUT9_000171"
     CDS             complement(26994..27533)
                     /locus_tag="ACHUT9_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1346751.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(27760..29064)
                     /gene="ndh"
                     /locus_tag="ACHUT9_000172"
     CDS             complement(27760..29064)
                     /gene="ndh"
                     /locus_tag="ACHUT9_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFR1346752.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(29472..30014)
                     /gene="ycfP"
                     /locus_tag="ACHUT9_000173"
     CDS             complement(29472..30014)
                     /gene="ycfP"
                     /locus_tag="ACHUT9_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFR1346753.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(30037..31062)
                     /gene="nagZ"
                     /locus_tag="ACHUT9_000174"
     CDS             complement(30037..31062)
                     /gene="nagZ"
                     /locus_tag="ACHUT9_000174"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFR1346754.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(31073..31897)
                     /gene="thiK"
                     /locus_tag="ACHUT9_000175"
     CDS             complement(31073..31897)
                     /gene="thiK"
                     /locus_tag="ACHUT9_000175"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFR1346755.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(31878..32519)
                     /gene="lpoB"
                     /locus_tag="ACHUT9_000176"
     CDS             complement(31878..32519)
                     /gene="lpoB"
                     /locus_tag="ACHUT9_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFR1346756.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(32533..32910)
                     /locus_tag="ACHUT9_000177"
     CDS             complement(32533..32910)
                     /locus_tag="ACHUT9_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFR1346757.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(32913..33272)
                     /gene="hinT"
                     /locus_tag="ACHUT9_000178"
     CDS             complement(32913..33272)
                     /gene="hinT"
                     /locus_tag="ACHUT9_000178"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFR1346758.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            33606..35795
                     /gene="fhuE"
                     /locus_tag="ACHUT9_000179"
     CDS             33606..35795
                     /gene="fhuE"
                     /locus_tag="ACHUT9_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFR1346759.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLVFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(35855..37288)
                     /gene="ptsG"
                     /locus_tag="ACHUT9_000180"
     CDS             complement(35855..37288)
                     /gene="ptsG"
                     /locus_tag="ACHUT9_000180"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFR1346760.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(37583..38380)
                     /locus_tag="ACHUT9_000181"
     CDS             complement(37583..38380)
                     /locus_tag="ACHUT9_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1346761.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(38391..39395)
                     /gene="holB"
                     /locus_tag="ACHUT9_000182"
     CDS             complement(38391..39395)
                     /gene="holB"
                     /locus_tag="ACHUT9_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFR1346762.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(39392..40033)
                     /gene="tmk"
                     /locus_tag="ACHUT9_000183"
     CDS             complement(39392..40033)
                     /gene="tmk"
                     /locus_tag="ACHUT9_000183"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1346763.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(40023..41045)
                     /gene="yceG"
                     /locus_tag="ACHUT9_000184"
     CDS             complement(40023..41045)
                     /gene="yceG"
                     /locus_tag="ACHUT9_000184"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFR1346764.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(41048..41857)
                     /gene="pabC"
                     /locus_tag="ACHUT9_000185"
     CDS             complement(41048..41857)
                     /gene="pabC"
                     /locus_tag="ACHUT9_000185"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFR1346765.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(41977..43218)
                     /gene="fabF"
                     /locus_tag="ACHUT9_000186"
     CDS             complement(41977..43218)
                     /gene="fabF"
                     /locus_tag="ACHUT9_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFR1346766.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(43306..43542)
                     /gene="acpP"
                     /locus_tag="ACHUT9_000187"
     CDS             complement(43306..43542)
                     /gene="acpP"
                     /locus_tag="ACHUT9_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFR1346767.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(43753..44487)
                     /gene="fabG"
                     /locus_tag="ACHUT9_000188"
     CDS             complement(43753..44487)
                     /gene="fabG"
                     /locus_tag="ACHUT9_000188"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFR1346768.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(44500..45429)
                     /gene="fabD"
                     /locus_tag="ACHUT9_000189"
     CDS             complement(44500..45429)
                     /gene="fabD"
                     /locus_tag="ACHUT9_000189"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFR1346769.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(45445..46398)
                     /gene="fabH"
                     /locus_tag="ACHUT9_000190"
     CDS             complement(45445..46398)
                     /gene="fabH"
                     /locus_tag="ACHUT9_000190"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFR1346770.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(46466..47536)
                     /gene="plsX"
                     /locus_tag="ACHUT9_000191"
     CDS             complement(46466..47536)
                     /gene="plsX"
                     /locus_tag="ACHUT9_000191"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFR1346771.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(47617..47790)
                     /gene="rpmF"
                     /locus_tag="ACHUT9_000192"
     CDS             complement(47617..47790)
                     /gene="rpmF"
                     /locus_tag="ACHUT9_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFR1346772.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(47842..48363)
                     /gene="yceD"
                     /locus_tag="ACHUT9_000193"
     CDS             complement(47842..48363)
                     /gene="yceD"
                     /locus_tag="ACHUT9_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFR1346773.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            48562..49146
                     /gene="yceF"
                     /locus_tag="ACHUT9_000194"
     CDS             48562..49146
                     /gene="yceF"
                     /locus_tag="ACHUT9_000194"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFR1346774.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENACLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(49258..50217)
                     /gene="rluC"
                     /locus_tag="ACHUT9_000195"
     CDS             complement(49258..50217)
                     /gene="rluC"
                     /locus_tag="ACHUT9_000195"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFR1346775.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            50790..53975
                     /gene="rne"
                     /locus_tag="ACHUT9_000196"
     CDS             50790..53975
                     /gene="rne"
                     /locus_tag="ACHUT9_000196"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFR1346776.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(54170..55123)
                     /gene="flgL"
                     /locus_tag="ACHUT9_000197"
     CDS             complement(54170..55123)
                     /gene="flgL"
                     /locus_tag="ACHUT9_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFR1346777.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(55135..56778)
                     /gene="flgK"
                     /locus_tag="ACHUT9_000198"
     CDS             complement(55135..56778)
                     /gene="flgK"
                     /locus_tag="ACHUT9_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFR1346778.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(56844..57785)
                     /gene="flgJ"
                     /locus_tag="ACHUT9_000199"
     CDS             complement(56844..57785)
                     /gene="flgJ"
                     /locus_tag="ACHUT9_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFR1346779.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(57785..58882)
                     /gene="flgI"
                     /locus_tag="ACHUT9_000200"
     CDS             complement(57785..58882)
                     /gene="flgI"
                     /locus_tag="ACHUT9_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFR1346780.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(58894..59592)
                     /gene="flgH"
                     /locus_tag="ACHUT9_000201"
     CDS             complement(58894..59592)
                     /gene="flgH"
                     /locus_tag="ACHUT9_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFR1346781.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(59645..60417)
                     /gene="flgG"
                     /locus_tag="ACHUT9_000202"
                     /pseudo
     CDS             complement(59645..60417)
                     /gene="flgG"
                     /locus_tag="ACHUT9_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(60589..61344)
                     /gene="flgF"
                     /locus_tag="ACHUT9_000203"
     CDS             complement(60589..61344)
                     /gene="flgF"
                     /locus_tag="ACHUT9_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFR1346782.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(61364..62569)
                     /gene="flgE"
                     /locus_tag="ACHUT9_000204"
     CDS             complement(61364..62569)
                     /gene="flgE"
                     /locus_tag="ACHUT9_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFR1346783.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(62594..63289)
                     /gene="flgD"
                     /locus_tag="ACHUT9_000205"
     CDS             complement(62594..63289)
                     /gene="flgD"
                     /locus_tag="ACHUT9_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFR1346784.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(63301..63705)
                     /gene="flgC"
                     /locus_tag="ACHUT9_000206"
     CDS             complement(63301..63705)
                     /gene="flgC"
                     /locus_tag="ACHUT9_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFR1346785.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNMVKSMMLKTLTLGQ"
     gene            complement(63709..64125)
                     /gene="flgB"
                     /locus_tag="ACHUT9_000207"
     CDS             complement(63709..64125)
                     /gene="flgB"
                     /locus_tag="ACHUT9_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFR1346786.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            64280..64939
                     /gene="flgA"
                     /locus_tag="ACHUT9_000208"
     CDS             64280..64939
                     /gene="flgA"
                     /locus_tag="ACHUT9_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFR1346787.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGNGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            65015..65308
                     /gene="flgM"
                     /locus_tag="ACHUT9_000209"
     CDS             65015..65308
                     /gene="flgM"
                     /locus_tag="ACHUT9_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFR1346788.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            65313..65729
                     /gene="flgN"
                     /locus_tag="ACHUT9_000210"
     CDS             65313..65729
                     /gene="flgN"
                     /locus_tag="ACHUT9_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFR1346789.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(65769..67304)
                     /gene="murJ"
                     /locus_tag="ACHUT9_000211"
     CDS             complement(65769..67304)
                     /gene="murJ"
                     /locus_tag="ACHUT9_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFR1346790.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(67414..68337)
                     /locus_tag="ACHUT9_000212"
     CDS             complement(67414..68337)
                     /locus_tag="ACHUT9_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1346791.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(68339..68986)
                     /locus_tag="ACHUT9_000213"
     CDS             complement(68339..68986)
                     /locus_tag="ACHUT9_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFR1346792.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(68997..69581)
                     /gene="rimJ"
                     /locus_tag="ACHUT9_000214"
     CDS             complement(68997..69581)
                     /gene="rimJ"
                     /locus_tag="ACHUT9_000214"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1346793.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            69817..71025
                     /gene="mdtH"
                     /locus_tag="ACHUT9_000215"
     CDS             69817..71025
                     /gene="mdtH"
                     /locus_tag="ACHUT9_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFR1346794.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            71089..71736
                     /gene="grxB"
                     /locus_tag="ACHUT9_000216"
     CDS             71089..71736
                     /gene="grxB"
                     /locus_tag="ACHUT9_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFR1346795.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            71870..72430
                     /locus_tag="ACHUT9_000217"
     CDS             71870..72430
                     /locus_tag="ACHUT9_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1346796.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            72536..73582
                     /gene="pyrC"
                     /locus_tag="ACHUT9_000218"
     CDS             72536..73582
                     /gene="pyrC"
                     /locus_tag="ACHUT9_000218"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFR1346797.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLITCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            73656..73901
                     /gene="dinI"
                     /locus_tag="ACHUT9_000219"
     CDS             73656..73901
                     /gene="dinI"
                     /locus_tag="ACHUT9_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFR1346798.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        17927 a        19729 c        18535 g        17780 t
ORIGIN      
        1 aaacaacggg cgtgttatac gcccgtttca atatttaact catggagaga ttacatgttt
       61 tcgatgatcg cttcaccaaa ctctgaacat ttcagcagct tagcgccttc catcagacgt
      121 tcaaagtcat aagttacggt tttcgcgttg atagcacctt ccataccttt aacaattaaa
      181 tcagccgctt cagtccaccc catatggcgc agcatcatct cagcggagag gatgatagag
      241 cctggattta ctttgtcctg accggcatat ttcggcgcag taccgtgggt ggcttcaaac
      301 agggcgcatt cgtcaccgat gtttgcgcca ggggcgatac cgataccgcc aacctgcgct
      361 gccagggcgt cagaaatgta gtcaccgttc aggttcatac aggcgataac atcatattca
      421 gccggacgca gcaggatctg ttgcaggaat gcatcagcaa tcacgtcttt aatgacgatc
      481 tctttgccgg tgttcgggtt tttaactttc agccacgggc cgccgtcgat cagttcaccg
      541 ccaaactctt cacgcgccag ctggtagccc cagtctttaa acgcgccttc ggtgaacttc
      601 atgatgttgc ctttgtgcac cagagtcaca gagtcacgat cgttagcaat tgcgtattcg
      661 atcgctgcac gaaccagacg tttggtgcct tcttccgaac acggcttaat accgatacca
      721 caatgttccg ggaagcgaat tttcttcacc cccatctctt cacgcaggaa tttaatcact
      781 ttctcggcgt cggcagagtc tgctttccat tcgatacccg cataaatgtc ttccgagttt
      841 tcacggaaga taaccatatc ggtcagttca gggtgtttaa ccgggcttgg agtgccctga
      901 tagtaacgta ccggacgcag gcagatgtag agatccagtt cctggcgcag ggcaacgttc
      961 agagagcgaa taccgccacc aacaggagtg gtcagcggac ctttaatggc aacgcgatat
     1021 tcacgaatca gatcaagggt ttcagcaggc agccagacat cctgaccata aacctgtgtg
     1081 gatttttcac cggtgtaaat ttccatccag gagattttac gctcgccttt ataggctttc
     1141 tcgactgcag cgtcgaccac tttcagcatg gctggggtta catctacacc gattccatca
     1201 ccttcaatgt aagggataat cggattttca ggaacgttga gtttgccgtt ttgcagggtg
     1261 atcttcttgc cttgtgccgg aacaactact ttactttcca ttcacctctc cttcgagcgc
     1321 tactggtttg ctcgtctgcc accacgttgc atatgcgttt gcgtcctgcg atacggatgc
     1381 tttagagcaa ttttttgtta atgatttgta attggcttgt caatgctacc ctattgtttg
     1441 ccgtcatgaa agatgcgcgt tattaggcta taatgcggca attcataatc tctgaaaata
     1501 ccatgcaaaa aacttctttt agaaatcacc aggttaagcg attcagctcg caacgttcta
     1561 ctaggcgtaa acctgaaaac cagcccacgc gtgtgatcct gttcaataaa ccctacgatg
     1621 ttcttccgca gttcaccgat gaagccggac gcaaaacatt aaaagaattc atcccggttc
     1681 agggcgttta tgcagcaggt cgtcttgacc gcgatagcga agggttactg gtgctgacca
     1741 ataacggcgc gttgcaggcg cgtttaaccc agccgggcaa acgcaccggg aaaatctatt
     1801 atgtgcaggt ggaaggtatt cccacacaag acgcacttga agccttgcgc aatggcgtaa
     1861 ccttaaatga tggccctacc ctgcccgccg gcgcggagct ggttgacgaa ccagcgtggt
     1921 tatggccgcg gaatccacca attcgtgaac gcaaaagtat tcccaccagc tggctgaaga
     1981 tcaccttata tgaaggacgt aatcgccagg tgcgccgcat gaccgcccat gttggtttcc
     2041 ccacgctgcg actgattcgt tatgcgatgg gtgattacac tttggataac cttgccaatg
     2101 gcgaatggcg agaagcaaca gaataaggat gcacaatgtt taaaccgcac gttaccgttg
     2161 cttgcgtagt gcacgcagag ggtaaatttt tagtcgttga agagacgatt aatggtaaag
     2221 cgttatggaa ccaacctgcc gggcatctgg aagccgatga aaccttagtg gaagctgccg
     2281 cccgtgagct atgggaagaa accggcatca gcgcgcaacc gcaacacttt attcgtatgc
     2341 atcagtggat tgcgccagat aaaacgccgt ttttgcgttt cctctttgcc attgagcttg
     2401 agcaaatatg cccgacccag cctcatgaca gcgatatcga ctgctgtcgt tgggtcagcg
     2461 ccgaagaaat tttaaaggcg tcaaatcttc gttcgccgct ggtggcggaa agtatccgtt
     2521 gttatcaaag tgggcaacgc tatccgctgg agatgattgg cgattttaac tggcctttta
     2581 caaagggtgt catctaaaag gctgcgtgat agaatacgcc gccttgaagt tcaatgtcgt
     2641 gagtgatcca atgtctgaaa ccgcaaaaaa agtaatcgtc ggcatgtccg gcggtgtcga
     2701 ttcctccgtt tctgcctggc tgttgcaaca acagggatat caggttgaag gcctgtttat
     2761 gaagaactgg gaagaagacg acggtgagga atattgcaca gcggcagcgg atctggctga
     2821 tgcccaggct gtctgcgaca agctcggcat tgaactgcac accgttaact ttgctgccga
     2881 gtactgggac aatgtcttcg aactgttcct tgccgaatat aaagccggtc gcacgccgaa
     2941 tccggatatt ctgtgcaaca aagagatcaa atttaaagct ttcctcgaat ttgccgccga
     3001 agatttaggt gccgattata tcgctaccgg tcattacgta cgtcgggccg atgtcgatgg
     3061 caagagccgc ctgctgcgtg gtctggacag caataaagac cagagctact tcctttatac
     3121 gctcagccat gagcagattg cgcaaagcct gttcccggtc ggcgaactgg aaaaaccgca
     3181 ggtgcgtaag attgctgaag atcttggtct ggtcaccgcg aagaaaaaag actctaccgg
     3241 catttgcttc attggcgaac gtaaattccg cgagttcctg ggccgttatc tcccggggca
     3301 accgggcaaa atcattaccg tcgatggcga tgaaattggt gagcaccagg ggctgatgta
     3361 tcacactctc ggtcagcgta aaggtctggg tatcggtggc accaaagaag gcaccgaaga
     3421 accttggtat gtggtggaca aagacgtcga aaacaacatt ctggttgtcg ctcagggcca
     3481 tgaacacccg cggctgatgt ctgtcgggtt gattgcccag cagttgcact gggtcgatcg
     3541 cgaaccattc accggcacta tgcgttgcac ggtaaaaacc cgctatcgcc agaccgatat
     3601 cccttgcacc gtcaaggcgc tggacgatga tcgcattgaa gtgattttcg atgaaccggt
     3661 tgccgccgtg acgccgggcc agtctgccgt cttctataac ggtgaagtgt gcctcggtgg
     3721 cggtattatt gagcagcgtc tgccgctgcc ggtctgatta ttatctttac ttaaaacaag
     3781 gaagcagtga acgtggcaaa gaattactat gacatcaccc tcgccctggc cggtatttgt
     3841 cagtcggcac gcctggtgca acaactcgct caccaggggc attgtgatgc cgatgcgcta
     3901 cacgtctcac tcaacagtat tattgatatg aaccccagct cgacgctggc ggtttttggc
     3961 ggtagcgaag ccaacctgcg cgtcgggctg gaaaccctgc tcggcgtgct caatgccagc
     4021 agtcgccagg gcttaaacgc cgaattaacc cgctacacac tcagcttgat ggtgcttgag
     4081 cgcaaactct cctcagcgaa aggcgcgctc gacactctgg gcaaccggat caacggcctg
     4141 caacgccagc tcgaacactt cgatttacag tccgaaacgc tgatgagcgc gatggctgct
     4201 atctatgttg atgtgatcag cccgcttggc ccacgcattc aggtcaccgg ttcccctgct
     4261 gtactgcaaa gcccacaagt gcaggcgaaa gttcgcgcaa ccctgctggc aggcattcgc
     4321 gccgccgtgc tctggcacca ggtcggcggc ggacgtctgc aactgatgtt ttctcgtaat
     4381 cgcctgacca ctcaggcaaa acaaattctt gctcatttaa ccccggagtt gtgatctatg
     4441 gaattatcct cactgaccgc cgtttcccct gtcgatggac gctacggcga taaagtcagc
     4501 gcgctgcgcg ggattttcag cgaatatggt ttgctgaaat tccgtgtaca agttgaagta
     4561 cgttggctgc aaaaactggc cgcgcacgca gcgatcaagg aagttcctgc ttttgctgcc
     4621 gacgcaatcg gttaccttga tgcaattgtc gccaatttca gcgaagaaga tgccgcacgc
     4681 atcaaaacca tcgagcgtac cactaaccac gacgttaaag cggttgagta tttcctgaaa
     4741 gaaaaagtgg cggagatccc ggaactgcac gcggtttctg aattcatcca ctttgcctgt
     4801 acttcggaag atatcaataa cctctcccac gcattaatgc tgaaaaccgc gcgtgatgaa
     4861 gtgatcctgc cgtactggcg tcaactgatt gatggcatta aagatctcgc cgttcagtac
     4921 cgcgatatcc cgctgctgtc tcgtacccac ggtcagccag ccacgccgtc aaccatcggt
     4981 aaagagatgg caaacgtcgc ctaccgtatg gagcgccagt accgccagct taaccaggtg
     5041 gagatcctcg gcaaaatcaa cggcgcggtc ggtaactata acgcccacat cgccgcttac
     5101 ccggaagttg actggcatca gttcagcgaa gagttcgtca cctcgctggg tattcaatgg
     5161 aacccgtaca ctacccagat tgaaccgcac gactacatcg ccgagctgtt tgattgcgtt
     5221 gcacgcttca acaccattct gatcgacttt gatcgtgacg tctggggtta tatcgccctt
     5281 aaccacttca aacagaaaac cattgctggt gagattggtt cttccaccat gccgcataaa
     5341 gttaacccga tcgacttcga gaactccgaa gggaatctgg gcctttccaa cgcggtatta
     5401 cagcatctgg caagcaaact gccggtttcc cgctggcagc gtgacctgac cgactccacc
     5461 gtgctgcgta acctcggcgt gggtatcggt tatgcgctga ttgcgtatca atccaccctg
     5521 aaaggcgtga gcaaactgga agtgaaccgt gaccatctgc tggatgagct ggatcacaac
     5581 tgggaagtgc tggctgagcc aatccagaca gttatgcgtc gctatggcat cgaaaaaccg
     5641 tacgagaagc tgaaagagct gactcgtggt aagcgcgttg acgccgaagg catgaagcag
     5701 tttatcgacg gtctggcgtt gccagaagaa gagaaagccc gcctgaaagc gatgacgccg
     5761 gcaaactaca ttggtcgcgc catcaccatg gttgatgagc tgaaataaac tcgtatcagt
     5821 gccggatggc gatgctgtcc ggcctgctta ttaagattat ccgcttttta ttttttcact
     5881 ttacctcccc tccccgctgg tttatttaat gtttaccccc ataaccacat aatcgcgtta
     5941 cactatttta ataattaaga cagggagaaa taaaaatgcg cgtactggtt gttgaagaca
     6001 atgcgttgtt acgtcaccac cttaaagttc agattcagga tgctggtcat caggtcgatg
     6061 atgcagaaga tgccaaagaa gccgattatt atctcaatga acatttaccg gatattgcga
     6121 ttgtcgatct cggattgcca gacgaggacg gtctgtcact gattcgccgc tggcgtagca
     6181 atgatgtttc actgccgatt ctggtattaa ccgcccgtga aagctggcag gacaaagtcg
     6241 aagtattaag tgctggtgct gatgattacg tgactaaacc atttcatatt gaagaggtga
     6301 tggcgcgaat gcaggcatta atgcggcgta atagcggtct ggcttcacag gtcatttcgc
     6361 taccaccgtt tcaggttgat ctctctcgcc gtgaattatc tattaatgac gaagtgatca
     6421 aactgaccgc gttcgaatac accattatgg aaacgttgat acgcaataat ggcaaagtgg
     6481 tgagcaaaga ttcgttaatg ctccaactct atccggatgc ggagctgcgg gaaagccata
     6541 ccattgatgt actgatggga cgtctgcgca aaaaaattca ggcacaatat ccgcaagaag
     6601 tgattactac cgttcgcggc cagggctatt tgttcgaatt gcgctgatga aaaaattact
     6661 gcgtcttttt ttcccgctct cgctgcgggt acgttttctg ttggcaacgg ctgcggtagt
     6721 actggtgctt tcgcttgcct acggaatggt cgcgctgatc ggttatagcg tcagtttcga
     6781 taaaactacg tttcggctgt tacgtggcga gagcaatctg ttctataccc ttgcgaagtg
     6841 ggaaaacaat aagttgcatg tcgagttacc cgaaaatatc gacaagcaaa gccccaccat
     6901 gacgctaatt tatgatgaga acgggcagct tttatgggcg caacgtgacg tgccctggct
     6961 gatgaagatg atccagcctg actggctgaa atcgaatggt tttcatgaaa ttgaagcgga
     7021 tgttaacgat accagcctct tgctgagtgg agatcattcg atacagcaac agttgcagga
     7081 agtgcgggaa gatgatgacg acgcggagat gacccactcg gtggcagtaa acgtctaccc
     7141 ggcaacatcg cggatgccaa aattaaccat tgtggtggtg gataccattc cggtggagct
     7201 aaaaagttcc tatatggtct ggagctggtt tatctatgtg ctctcagcca atctgctgtt
     7261 agtgatcccg ctgctgtggg tcgccgcctg gtggagttta cgccccatcg aagccctggc
     7321 aaaagaagtc cgcgaactgg aagaacataa ccgcgaattg ctcaatccag ccacaacgcg
     7381 agaactgacc agtctggtac gaaacctgaa ccgattgtta aaaagtgaac gcgaacgtta
     7441 cgacaaatac cgtacgacgc tcaccgacct gacccatagt ctgaaaacgc cactggcggt
     7501 gctgcaaagt acgctgcgtt ctctgcgtag tgaaaagatg agcgtcagtg atgctgagcc
     7561 ggtaatgctg gagcaaatca gccgcatttc acagcaaatt ggctactacc tgcatcgtgc
     7621 cagtatgcgc ggcgggacat tgctcagccg cgagctgcat ccggtcgccc cactgctgga
     7681 caatctcacc tcagcgctga acaaagtgta tcaacgcaaa ggggtcaata tctctctcga
     7741 tatttcgcca gagatcagct ttgtcggtga gcagaacgat tttgtcgagg tgatgggcaa
     7801 cgtgctggat aatgcctgta aatattgcct cgagtttgtc gaaatttctg caaggcaaac
     7861 cgacgagcat ctctatattg tggtcgagga tgatggcccc ggtattccat taagcaagcg
     7921 agaggtcatt ttcgaccgtg gtcaacgggt tgatacttta cgccctgggc aaggtgtagg
     7981 gctggcggta gcccgcgaaa tcaccgagca atatgagggt aaaatcgtcg ccggagagag
     8041 catgctgggc ggtgcgcgga tggaggtgat ttttggtcgc cagcattctg cgccgaaaga
     8101 tgaataaata tgtccatact tcacgcatta cgttaagcat ccgttataat cggttgcaga
     8161 taccagcctg tggatgctta acatggaata ccaactcact cttaactggc ccgattttct
     8221 tgaacgtcac tggcagaaac gcccggtggt gttaaaacgc ggctttaata attttattga
     8281 cccgatctct ccagacgagt tggcgggtct ggcgatggaa agcgaagttg acagtcgact
     8341 ggtcagtcac caggatggca aatggcaggt cagccacggc ccgttcgaaa gctacgatca
     8401 tctcggtgaa accaactggt cattactggt acaggcagtg aaccactggc atgagccgac
     8461 cgccgcgctg atgcgaccgt tccgtgaact accggactgg cgtattgatg atctgatgat
     8521 ttctttttct gtacctggcg gcggcgtcgg cccgcatctc gatcagtacg acgtgtttat
     8581 cattcagggt accggacgtc gtcgctggcg agtgggcgaa aagctgcaaa tgaaacagca
     8641 ctgcccacat ccggatctgt tacaggtcga tccgttcgaa gccatcatcg atgaagagct
     8701 ggagcctggt gatattcttt atattccgcc aggattcccg catgaaggct acgcgctgga
     8761 aaatgcgatg aactattccg tgggctttcg cgcgccaaat acgcgggaac tgattagtgg
     8821 atttgccgat tatgtgctgc aacgtgaact gggcggcaac tactacagcg atcctgatgt
     8881 tccacctcgc gctcatcctg cggatgttct gcctcaagag atggataaac tgcgtgagat
     8941 gatgctcgaa ttgatcaacc agccggaaca ctttaagcaa tggtttggcg agtttatatc
     9001 ccagtcacgt catgaactgg atatcgcgcc gccagaaccg ccttatcagc cagatgaaat
     9061 ctacgatgcg ctgaaacaag gtgatgtgct ggtgcgcctg ggtggtctgc gcgtattgcg
     9121 cattggcgac gacgtgtatg ccaatggtga gaagatcgat tccccgcacc gtccggcact
     9181 ggatgcactc gccagcaaca ttgcgctgac tgcggagaat tttggcgatg cgctggaaga
     9241 tccgtcattc ctcgcgatgc tcgcggcgct ggtcaatagc gggtattggt tcttcgaagg
     9301 gtaagtttgg atttaagccg gatgcggcat cacatgccgc atcccttttc gcttacttcc
     9361 gttgcgccgt taactcggca acacggacga tcacctgcac cgctttttcc ataccttcca
     9421 gagtcacaaa ctcatgctta ccatgatagt tgtaaccgcc agtgaacaag ttcgggcacg
     9481 gtaatcccat aaacgacaac tgcgcgccgt cggtaccacc gcggatcggt ttcagttccg
     9541 gttcaatatc gcagtcacgc atcgcctgct gggcgatatc gagaatatgc ggatgctcaa
     9601 ccactttctc tcgcatattg tagtaactgt cttcaatcac cagttcaatg tagcaatcag
     9661 gatgtaaccc tttgcccact tttttggcga tctccatcat tttacgttta cgcgcttcaa
     9721 actgtttacg gtcgaaatca cggatgatgt agtgcatatc ggcccgttca acggtgcctt
     9781 tcatgctcgc caggtgatag aaaccttcat agccttctgt catttccggg ctttcatccg
     9841 ccggaacttc cgcatgaata cgtgccgcca gcgacagcgc atttaccatc actcctttcg
     9901 ccgtgcccgg atgaacattg ttaccgacaa ttttgatatt gaccgatgcg gcgttgaagt
     9961 tttcaaactc cagttcgcct acgccaccac cgtcaacagt gtaagcccag cgggcatcga
    10021 aggcatcaac atcaaaatgt ttcgcccctt tgcccacttc ttcatccggg gtaaaggcga
    10081 cgcgaatatc accatgcgga atgtttttct gttgcaatac cgccagcgcg gtcatgattt
    10141 ctgcaatacc tgctttgtca tcggcaccta acaaggtttt accatcggtg gtaatcaacg
    10201 tctgacccag tagctgatgc agcaccggga acataaccgg tgataaaact tcatcgccga
    10261 tacccagcgc aatatcgcca ccgcgatagt tttcaacaat ttgcggatta acatttttgc
    10321 cgctgcaatc cggtgaggta tccacatgag aaataaagcc aatcgccggg atatcgccag
    10381 ggacgttagc cggtaacgtc gccatcaaag tgcccttctc acttaaggtc acattgataa
    10441 gccccatttc ttcgagctgc tctttcagca gatgcaataa cttccattgg ccttccgtgc
    10501 tgggaacctg tctcacccct gcttttgatt gggtatccag agatacgtag ttcaaaaatc
    10561 gctcaagtag tttatccatg tagtcaccct cactttttgt gacaacatta ttaagaagca
    10621 agaaaagaca aatattgcgt caggtcactt ttacccctgc aagcgggaat atttatcagc
    10681 attaccttaa tgaatataaa gctaagccag taattcacaa caaagattgg cgattcaagc
    10741 ggtttgccgt agaattaccg ccctcattaa gagtcaccaa ggtggttaac cacaaacccc
    10801 gcatcggtaa gccatccgtt gcgtttacat gggacagagt aaaaaattga ataaacaacc
    10861 gagttcgctt tcaccgctgg tgcaattggc gggaattcgc aaatgctttg atggtaaaga
    10921 ggtcattccc cagctggatc tgactatcaa caatggcgag ttcctcacgc tgcttggccc
    10981 ttctggctgc ggtaaaacaa ccgttcttcg cctgattgca ggtctggaaa ctgttgattc
    11041 cggacgcatc atgctggata acgaggacat cacccacgtt ccggcggaaa accgctatgt
    11101 gaacactgtt ttccaaagct acgcactttt cccccacatg accgtgttcg aaaatgtggc
    11161 ctttgggttg cgcatgcaaa aaacccccgc tgctgaaatt acgccccgcg tgatggaagc
    11221 cctgcggatg gtgcaactgg aaaccttcgc tcaacgtaaa ccgcatcagc tctctggtgg
    11281 tcaacagcaa cgcgtcgcca ttgctcgcgc ggtggttaac aagcctcgtc tgttgttgct
    11341 ggatgagtcg ctctcagcgc tggattacaa actgcgtaag caaatgcaga acgagctgaa
    11401 agcgttacag cgtaagcttg gtattacctt cgtctttgtc actcacgatc aggaagaagc
    11461 gctcaccatg tcagacagga ttgtggtgat gcgcgagggt cgtattgagc aagatggtac
    11521 accgcgcgaa atttacgaag agccgaaaaa cctgtttgtt gccggcttca ttggcgaaat
    11581 caatatgttt aacgccactg tcatcgaacg tctcgacgag cagcgcgtac gcgccaacgt
    11641 tgaaggccgc gaatgtaata tctacgttaa cttcgccgtt gaaccggggc aaaaactgca
    11701 tgttctgctg cgcccggaag acttacgtgt tgaagagatt aacgacgaca accacgctga
    11761 agggctgatt ggttacgttc gtgagcgtaa ctacaaaggc atgacgctgg agtcggttgt
    11821 tgaactggaa aatggcaaga tggtgatggt cagcgaattc ttcaatgaag acgaccctga
    11881 ctttgaccac tctctcgacc aaaaaatggc cattaattgg gtagaaagct gggaggtcgt
    11941 actggctgat gaagaacaca agtaagttcc agaatgtagt gattgtcact attgtcggtt
    12001 ggcttgtgtt gtttgtcttt ctgcccaacc tgatgatcat tggcaccagc tttttgaccc
    12061 gcgacgacgc cagtttcgtc aaaatggtct ttacgctgga taactacacg cgtctgctcg
    12121 atccgctcta ttttgaagtg ctattgcact cgctgaatat ggcgctgatc gccaccctcg
    12181 cctgcctggt gctgggctac ccgtttgcct ggtttctggc gaagctgcca cacaaggtgc
    12241 gtccgctgct gctgtttctg ctgattgttc ccttctggac caactcactg attcgcatct
    12301 acgggctgaa aattttcctc agcaccaaag gctatctcaa cgagtttttg ctctggctgg
    12361 gcgttatcga cacaccaatt cgtatcatgt tcacgcccag tgcggtgatt atcggtctgg
    12421 tttacattct gctaccgttt atggtgatgc cgctgtactc cagtatcgaa aaactggata
    12481 agccgttgct tgaggcggcg cgcgatctcg gtgccagcaa gttacagact tttatccgta
    12541 tcattattcc actgacgatg ccgggaatta ttgccggatg tctgctggtg atgctgccag
    12601 cgatgggcct gttctatgta tccgacctga tgggcggtgc gaaaaacctg ctgatcggta
    12661 acgtcatcaa ggtccagttc ctcaatattc gtgactggcc gtttggtgca gccaccagca
    12721 ttacgctgac tatcgtaatg ggcctgatgt tgctggttta ctggcgcgct tctcgtctgc
    12781 tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt cgcggcggtt ttatgaccgc
    12841 tatctacgcg tacctgtata tcccaatcat tattttgatt gtgaactcct ttaacagctc
    12901 gcgctttggc atcaactggc agggttttac caccaaatgg tatagcctgc tgatgaacaa
    12961 cgacagcctg ttacaggcag cgcagcattc actaacaatg gcggtgtttt cggcgacatt
    13021 tgctacgctt atcggttcac tgacggcagt tgcgctgtac cgttatcgct ttcgtggtaa
    13081 gccgttcgtt agcggaatgc tgtttgtggt gatgatgtca ccagatatcg tgatggcgat
    13141 ttctctgctg gtgctgttta tgctgctggg tattcagctt ggcttctggt cgctgctgtt
    13201 ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg gtgtattcgc gcctgaaagg
    13261 ttttgacgtg cggatgctgg aagcggcgaa agatctcggt gccagtgaat ttaccattct
    13321 gcgaaaaatc attctgccac tggcaatgcc agcggtggcg gcgggctggg tgttaagctt
    13381 taccctgtcg atggacgatg tggtagtttc ttcgttcgtc accggaccaa gttatgaaat
    13441 tctgccgtta aaaatttatt cgatggtcaa agtcggcgta tcgccggaag ttaacgcgct
    13501 ggcaaccata ttactggtgc tgtcgctggt gatggttatt gccagccagc ttattgctcg
    13561 tgataaaacg aaaggtaaca caggggacgt taaatgaaaa aatggtcacg ccacctgctc
    13621 gcggcgggtg ctctggcact gggcatgagc gccgctcacg ccgatgacaa caacacgctg
    13681 tatttctaca actggaccga gtacgtgccg ccaggactgc ttgaacagtt caccaaagaa
    13741 accggtatta aggctatcta ttcgacttac gagtcgaacg aaaccatgta cgccaagctg
    13801 aaaacgtaca aagacggtgc ctatgacctg gtggttcctt caacctatta cgtcgataaa
    13861 atgcgtaaag aagggatgat ccagaagatc gacaagtcga agttaacgaa cttcagcaat
    13921 ctcgatccag acatgctcaa caagccgttt gacccgaata acgactactc cattccgtat
    13981 atctggggtg cgacggcgat tggcgttaac ggtgatgcgg tggatcctaa atctgtcacc
    14041 agttgggcag atctgtggaa gccagagtac aaaggtagcc tgctgctgac cgacgatgcc
    14101 cgtgaagtgt tccagatggc gctgcgcaaa ctgggctact ccggtaacac caccgatccg
    14161 aaagagattg aagctgcata taacgagctg aaaaaactga tgccaaacgt cgcagcgttt
    14221 aactccgata acccggctaa cccgtacatg gaaggcgaag ttaacctcgg catgatctgg
    14281 aacggctctg ctttcgttgc acgccaggcg ggtacgccaa ttgacgtggt gtggccgaaa
    14341 gaaggcggca ttttctggat ggacagcctg gcgatcccgg caaatgccaa aaacaaagaa
    14401 ggcgcgctga aattgatcaa cttcctgctg cgcccggatg tggcaaaaca ggttgccgaa
    14461 actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc tgttaagccc ggaagtggcg
    14521 aacgataaaa cgctctaccc ggatgctgaa accattaaaa atggtgaatg gcagaatgac
    14581 gttggcgcag ccagcagcat ttatgaagag tattatcaga agctgaaagc aggacgttaa
    14641 ttcagcaaac gggcggtggt attaccgccc gttttttagt tagcaaggat gtggctaatg
    14701 agtcaggaca gtaaagtttt atcccgctct ttcgtcggta ttgtggtccc cttgtaggcc
    14761 tgataagcgt agcgcatcag gcaatgcttc ctgctttata atcccttcag caacttttca
    14821 acaaactcag gcaccacctg gcttgccggg ccgtaatatt tctcggcaaa ttcattaccg
    14881 acctgacttg gttcgagatt cagttccacg gtgtgcgcgc catgcagttt cgcttcgtga
    14941 acaaacccag ccgccggata aacatgcccg gaagtaccaa tggcaatgaa aatatcagcc
    15001 atcgacaacg ccatataaat ttcatccatg ccgagtggca tttcgccaaa ccaaacgacg
    15061 tgcgggcgca agggtgccgg gaactggcaa caatggcatt tatcttctgg cgtaacgtct
    15121 ccggtccagt cgagaacctg accactttgc gaacaacgaa ctttcagcaa ttccccatgc
    15181 atatgaatga cattggtatt acctgcgcgt tcatgcaggt tgtctatatt ctgcgttacc
    15241 agcaaaaagc gatcgccgag ggcgtcttgc agtttagcca gcgcaagatg cgcggcgtta
    15301 ggctgaattt ctggctgctg cagctgtcga cgacgggcgt tataaaacgc ttgcaccagt
    15361 tcaggatcgc gatcgaaacc ttccggcgtt gccacatctt caacccgatg ttcttcccac
    15421 aggccatctg cggcgcgaaa ggtacgaata cctgattccg cagaaattcc tgcccctgtc
    15481 agtacgagta ctcttggttt ttccattgct tccggcacca ctttatctct gaaaaaaata
    15541 cgctgacgca aacgctcgcg caggcggcgt ttatttttac gaaaacgact taaccgatga
    15601 ccccgacgcg acagcatagc aacctctgtt gtttaatcgg ttagatgtag gaaggccgca
    15661 ccacgcattc ctcccgcatc accgtggcgc gcgcgttcaa tgcgcggaac acgagctaca
    15721 ggtaagagat gacgaggcag cctgtccgcc agttgcgttg tgattgccgg gaaattcgat
    15781 aagccaccac caatgacgac caggtcagag tcaacgatgg tcaggatatt tcccagacaa
    15841 accgctaata aatccagata acgctcaacg tgcgcccttg cctgctcatc gccttgatca
    15901 taaagcgcaa taatttcggg agcctgcaac ggttgatgat aatagtgttg ccagagccac
    15961 gcaaagcccc gacctgatag ataattttcg atacaaccaa tttgaccaca gccgcagcgg
    16021 cgtaacggga aatccagccc catcatggtt aacgcatcaa ccggcagacg catatggcca
    16081 aactcgccgg taatgtagct tttaccggta atcggtttac cgttgaaaat cagcccgccg
    16141 ccaacgccgg tgccgagaat taaccccatc accagtggat attgcgtaaa ttcatcatcc
    16201 caggcttctg aaagggcaaa acagttggca tcgttatcaa ggcgtacatc gcgatcaaga
    16261 cgtgcgctca ggtcggcacg cagcggttta ccgctggcag cggggacatt ggcggcatac
    16321 agcgtaccat cttctgtttc cggcattccc ggaataccga tgccaacaga acctttacag
    16381 ccaaaacgtt ggtcagcttc ggctaccagc tcgcatactg catctaaaaa tgcgtcatag
    16441 ctgtcacgcg gtgtcggcac ccgcttttcc cactgcaact gccgaccgct atcaaacacg
    16501 ccaagcgcaa tttttgttcc accaatatca aacccgtaat acattgccgc tccttgtttt
    16561 aatgtactgc ctttactggc cgctaaggac tcgcgcaggg tcaatattac tggcgcgccg
    16621 cgccggatac caacttgcca aaagactcag caacaatgct gtgaccagta cgtagaagac
    16681 gtccagccag tgcaattccg atggcaggaa gtcaataaaa tagatatcgc tggagaggaa
    16741 ctgatgaccg atcagctttt caatccgctc aataatcggg gtaagttgca gtgaaacaac
    16801 tacgccgata atcacaccac acaggctacc gaacagccct gccagcaatc cataccagac
    16861 aaagatggcg cgaattaaac catctttcgc ccccagcgtt cttaatactg cgatatcgcc
    16921 actcttgtct ttcaccgcca tcactaaggt ggagacgatg ttgaaacagg ccacgccaat
    16981 caccagtacc atcgccagat acataatggc gcggatcatc tggatatcgc gatacatata
    17041 gccgtaagta ccaatccaac ttttaatata aacatagctg ttggtcactt cacccgcatc
    17101 gcgtaccagc ttattggcgt tgaaaacatc cgtcatttta agggcaatac ctgacacgct
    17161 ggaacccata tcaagatatt gttgggcatc cgccagcggg atcatggcaa aactgtgatc
    17221 gagttgacca ctcaactgca aaataccggc aatgtgcaaa cgcacacgtt ttggctgcat
    17281 cagtttatgc tcgggattcg agttggggat catgatcgac acccaatcgc cctgcttcac
    17341 tttcagcgca tccgccacgc ctttgccgat gataatttgc tgttcgcccg ctttaaaatt
    17401 gcgccaggca tccccctgaa caaacgaggg taatgcgctc agacgctgtt cctgttgcgg
    17461 gttaacgccc ttcacctgaa ttgcgcgcag attggtacca ctttctacca gtccggtgaa
    17521 attgatatac ggcgcagcgg cggcaatacc cggcactttt tgcacgttat ccagtgcttc
    17581 ctgccagtta gtccatggct gatttaccgc ctcaatttca ccgtgcggta ccacagcaag
    17641 aatgcggtta ttcagttcgc gttcaaagcc gttcatcgcg cttaagccga cgatcaatac
    17701 cgccacgccg agggcaatgc caatggtaga aatgacagag attagcgaca ccatgccgcc
    17761 gcgtcgccgt ccgcgactaa aacgcaggcc aatcaataac gataaaggca tcgccattac
    17821 tccgccccca tcaggctcag ttccgccgtc agacgtccat cacgcatttc cagttggcgg
    17881 ctcatacgtt tcgccagttg caggtcgtga gtaaccacca ggaaggcggt gccctgcaag
    17941 cgattcaatt ccccaagcaa ctggaagatg ctgtcggcgt tacgcgcatc gaggttaccg
    18001 gtaggctcat ccgcaagtac cagacgcgga ttattgacca gcgcacgggc aatcgccaca
    18061 cgctggcgtt cgccgccaga aagttcagat gggcggtgat tcgcacgatg ctccagcccc
    18121 accgctttta acatctccag cgcacggctg ttgatttcag cgggcttttt cttgccaatc
    18181 agcagtggca tagccacgtt ttccagggca gtaaaatccg gcagcaggtg gtgaaactga
    18241 taaataaagc ccagcttctg gttgcgcagt tctgctttcg ccgccgaaga cagtttgctc
    18301 atcggttgac cgttaaagat cacatcgccg gaggttggtg tatccagccc gcccagcagg
    18361 tgcagcaagg tacttttacc ggaaccagag ctaccgacga tcgccatcat ttcaccttcg
    18421 ccgacgctga aactgacatt gtgcagtaca tcggtttgca cactgccttc ctgatagcgt
    18481 ttgcacaggt tgtcgcattg caacaggatc ttattcataa cgtaaagcct cagcgggttg
    18541 agtggcggca gcgcgccatg aagggtaaag cgtagacagc agcgcgatag ccatcgccac
    18601 cagcgcaata acaatgacct gtaaaggttc gatagcgacc ggcagcgccg cgccatcaag
    18661 caggacgccg attatcggca tcagattatt taactggctg gcaagcagtg cgccaagcgc
    18721 cgctccgagg atcgcaccga taatcccggc gctggcccct tgcaccataa agaccatcat
    18781 gatttgtcgc ggagttaagc cttgcgtttg caggatcgcg acttcgccct gcttctccat
    18841 taccatcagc cctagtgagg taataatgtt aaacgccgca acggcgacaa tcaggctcag
    18901 cagcaagccc atcatatttt tttccatgcg tacggcctgg aacagctcgc ctttacggtc
    18961 acgccagtcc tgccatttgc tgccttcagg cagtttttgc tgacttaaag agtcaacttt
    19021 cagcggctca tccagccaca aacgccagcc ggtaatattg cctgccggat aacgcatcag
    19081 acgcgaggca tcctcaatat tcaccagcat ttcatagcca tcgacttcac tgttggcggc
    19141 gaaagtgcca atcacattga acaggcgctg gcttggaata cgccccatcg gcgtgaactg
    19201 gctggcagat ggtaccatca cgcggatttg atcaccgcga ttaacgccta gctgtgaggc
    19261 aagttgttcg ccgaggatga cattatattt ccccggctcg aggtcagttt gtttcacatt
    19321 gaccagatac ggcgtaagtg gatctttttg cgccgggtcg ataccgagca tcaccccgac
    19381 cgccacgctg cgcgcgcttt gcagtaccac atcaccggta gtaataggtg cgacgcgatt
    19441 aacgccgtcc agtttgactg ccgtttccgg gagttgctgc gggttaagag agccatgctc
    19501 agaagagaga attgcctgtg gcatcaggcc aaggatgttg ttttgcagct cgcgctcaaa
    19561 gccgttcatc actgacaata ctgtgaccag cgccatcacc ccgagggtaa tgccgatggt
    19621 agaaagccag gagacgaaac gaccgaagcg atccgctgca cgcccacgca tgtaacgcag
    19681 gccaataaat agagcgacag gttggtacat gaaatccgtc tggttgctgt agcaaagaca
    19741 cgagtatata agcgaaagca agatgggtaa acgtctgaac tgtaagtgat cgtcagggct
    19801 aatctgaaaa gcccccgtaa atcaatcggc taaattgcca tgttttatca tcaatcttgc
    19861 cgcctgatct taatctgaaa aatcatccaa tacagacaga tacccattac acctcgggtc
    19921 ataacccgca ggatcaaccg catgtaaatt aaaaaagagc gtgttgtcct gatgaaacaa
    19981 aaagagctat ggattaacca gatcaaaggg ttatgtattt gtctggtggt gatttatcac
    20041 tcggtcatta ccttttatcc gcatatgacc acttttcagc atccgttatc ggaagtcctg
    20101 agcaaatgct ggatctattt caatctttac cttgcaccct ttcgtatgcc ggtttttttc
    20161 tttatatccg gctatttgat tcgccgctat atcgacagcg taccgtgggg aaattgtctc
    20221 gataaacgga tctggaacat cttctgggtg ctggcgcttt ggggcgtggt gcagtggctg
    20281 gcgttaagtg cgctgaacca gtggctggca cctgagcgcg atttaagcaa tgcctccaat
    20341 gccgcttatg ccgattccac cggtgagttc ctgcacggga tgatcaccgc cagcaccagc
    20401 ttgtggtatc tgtatgcgtt aattgtctat ttcgtggtat gtaaaatttt tagccgtctg
    20461 gcgctgccac tattcgcctt gtttgtactg ctgagtgtgg cggttaattt cgttcccacg
    20521 ccgtggtggg gaatgaacag tgtgatccgc aatttgcctt attacagcct tggcgcatgg
    20581 tttggcgcaa caataatgac ctgtgttaaa gaggtgccgt tgcgccgcca tctgctgatg
    20641 gcttctttgc ttaccgttct ggcggtcggt gcctggttgt ttactatctc gctgctgttg
    20701 tcgctggtat cgattgtggt gatcatgaag ctgttttatc agtacgagca acgattcggt
    20761 atgcgctcca ccagcctgct gaatgtgatt ggctccaaca ccattgctat ctacaccacc
    20821 catcgcattc tggttgaaat attcagctta actctgcttg cgcaaatgaa cgcagcacgc
    20881 tggtcgccgc aagtcgaact gacactcctg ctggtttatc cctttgtcag tttgttcatc
    20941 tgtactgttg cgggcttgct ggtaagaaaa ctttcacagc gtgcattcag cgatctgttg
    21001 ttctccccgc cttcactgcc cgctgccgtc agttactctc gttaaaccat cgccccagcc
    21061 ggggcgattc aatttgctaa accatgtcga ttacggataa tgaacagcat tgcttatcag
    21121 agatatgccc ccatatgttg aggcatatcc taacgagaat ctgacaaccg ttatgcctga
    21181 acaatatcgt tatacactgc ccgtcaaagc gggtgagcag cgtctgctgg gcgagttaac
    21241 cggcgcagcc tgtgcaacgc tggtagcgga aattgccgaa cgtcacgccg gtccggtggt
    21301 actcattgca ccagatatgc aaaatgctct gcgtttgcat gatgaaatca gccagttcac
    21361 cgatcagatg gtgatgaatc tggcggactg ggaaactctt ccctacgaca gtttttcgcc
    21421 tcatcaggac attatctcct cgcgcctttc caccctttac cagctaccga cgatgcagcg
    21481 tggcgtactg attgttccgg tgaatacgct tatgcagcgc gtttgcccac acagttttct
    21541 ccacggtcat gcgctggtga tgaaaaaagg tcagcgcctg tcacgagatg cattacgaac
    21601 ccaactggac agcgccggtt atcgccatgt tgaccaggtg atggagcacg gcgaatacgc
    21661 cacgcgcggc gcgttgctgg atctcttccc gatggggagt gagctgcctt atcgtcttga
    21721 tttctttgat gatgaaatcg acagcctgcg ggtgtttgac gtcgacagcc agcgcacgct
    21781 ggaggaagta gaagcgatca atctgctgcc cgcgcacgaa tttccgaccg ataaagcggc
    21841 aattgaactg ttccgcagcc agtggcgcga taccttcgaa gtgaagcgcg atccggaaca
    21901 tatttaccag caagtgagta aaggcacatt acctgccggg atcgagtact ggcagccatt
    21961 gttcttcagc gaaccactgc cgccgctgtt cagttatttc cctgccaata ccttgctcgt
    22021 gaatactggc gatctggaaa ccagtgccga acgtttccag gctgacacgc tggcgcgttt
    22081 tgagaatcgc ggcgtcgatc cgatgcgccc gctgttgcca ccacaatcgc tctggctgcg
    22141 ggtggacgag ctcttctcag agctgaaaaa ctggccccgg gtgcagctaa aaactgaaca
    22201 tttaccgaca aaagccgcga atgccaattt aggtttccag aaactgccag acctggccgt
    22261 tcaggcacaa caaaaagcgc cgctggatgc gctgcgtaag ttcctcgaga ctttcgacgg
    22321 tccggtggtg ttctcggtag aaagtgaagg tcgccgtgaa gcgctgggtg aactgctcgc
    22381 acgaattaaa attgctccgc aacgcattat gcgtcttgat gaagccagcg accgtgggcg
    22441 ttatctgatg attggcgctg ccgaacatgg ttttgtcgat aaggtgcgta atctggcgct
    22501 gatctgcgaa agcgatctgc tcggtgaacg tgttgcccgc cgtcgtcagg attctcgccg
    22561 caccatcaac cccgatacac tgatccgtaa ccttgcggag ctgcatattg gtcagccggt
    22621 ggtccatctg gagcacggtg tcgggcgcta tgccggaatg accacgctcg aagcgggcgg
    22681 cattactggc gagtatttga tgctcaccta tgccaacgac gccaaactgt atgttccggt
    22741 gtcgtcactg catctgatta gccgttacgc aggtggcgcg gaagaaaacg ccccgctgca
    22801 taaacttggc ggcgatgcgt ggtcacgcgc gcggcagaaa gcggcggaaa aagtgcgtga
    22861 tgtggcggcg gaattgctgg atatctacgc gcaacgcgcc gccaaagagg gcttcgcgtt
    22921 taaacacgat cgtgagcagt atcagttgtt ctgcgacagc ttcccgtttg aaaccacgcc
    22981 ggatcaggcg caggccatta atgcggtact tagcgacatg tgtcagccgc tggcaatgga
    23041 tcgtctggtg tgcggcgatg ttggctttgg taaaacagaa gtggcgatgc gcgccgcttt
    23101 cctggcagta gataaccaca agcaggtggc ggtgttggtg cctaccaccc ttctcgcgca
    23161 gcagcattac gacaacttcc gcgaccgttt cgccaactgg ccggtacgta tcgaaatgat
    23221 ctcccgtttc cgcagcgcca aagagcagac gcaaatcctt gcggaagtgg cggaagggaa
    23281 aatcgatatt ctgatcggta cgcacaaact gctgcaaagt gacgtcaagt ttaaagattt
    23341 aggcctgctg attgtcgatg aagaacaccg cttcggggtg cgtcataaag agcgcattaa
    23401 agcgatgcgc gcgaacgtgg atattctgac gcttactgca acgccgatcc cacgcacgct
    23461 gaatatggca atgagcggaa tgcgtgacct gtcgattatc gccacgccgc ccgcccgtcg
    23521 tctggcagtt aaaacctttg tccgtgagta tgacagcctg gtggtccggg aggcgatcct
    23581 gcgtgaaatt ttgcgcgggg ggcaggttta ttatctctac aatgatgtgg aaaacatcca
    23641 gaaagctgcc gaacggctgg cagaactggt gccagaagcg cggatcgcca tcggtcacgg
    23701 gcagatgcgc gagcgcgaac tggaacgggt gatgaatgat ttccatcatc aacgtttcaa
    23761 cgtgctggtt tgtacaacca ttatcgaaac cgggatcgac atcccgacag ccaacaccat
    23821 tatcattgaa cgcgcggatc acttcggtct ggcgcagctg caccagttac gcggtcgcgt
    23881 cggacgttcg catcatcagg catatgcatg gttgctgaca ccgcatccaa aagcgatgac
    23941 taccgatgca caaaaacgtc ttgaagcgat tgcctcgctg gaagatctcg gggcaggttt
    24001 tgcgctggca acgcacgatc tggagattcg cggcgcgggt gaactgcttg gcgaagaaca
    24061 aagcggctca atggaaacca tcggtttctc gctgtatatg gagttgctgg aaaacgccgt
    24121 cgatgcactg aaagccggac gcgagccgtc gctggaagat ctcaccagcc agcaaacaga
    24181 agtcgagctg cggatgccgt cgctattgcc agatgatttc attcctgacg tgaatacgcg
    24241 tttgtcgttc tataaacgta ttgccagcgc caaaacggaa aacgaactgg aagagatcaa
    24301 agtcgagctt atcgatcgct tcggcctgct gccagatccg gcgcgtaccc tgctggatat
    24361 tgcccgtctg cgccagcaag cgcagaaact ggggatcagg aagctggaag gtaatgagaa
    24421 aggcggggtg atcgaatttg ccgagaagaa tcacgttaat ccggcctggt tgattggttt
    24481 gctgcaaaaa cagccgcagc attaccgcct tgatggtccg acgcgcctga aatttattca
    24541 ggatttgagt gagcggaaaa cgcgtatcga atgggtacgc cagtttatgc gtgaactgga
    24601 agagaacgcg atcgcttaat ggtaaatcgg gggcgtttct gcgcccccat ctgcaacatt
    24661 tacaaattct ttgcacttcc ctgcactatc cgacactgtc accatccata attcaggctt
    24721 atctgtttat tacaataacc ttatatttat tattgatttt tggtgatgat caaaacgcgt
    24781 ttttctcgct ggctaacgtt ttttacgttc gccgctgccg tggcgctacc ggcaaaagcc
    24841 aacacctagc cgctgccgcc agcgagcagt cgtctggttg gcgaaaacaa atttcatgtg
    24901 gtggaaaatg acggtggttc tctggaagcc atcgccaaaa aatataacgt cggctttctc
    24961 gctctgttac aggctaaccc cggcgttgat ccttacgtac cgcgcgcggg cagcgtgtta
    25021 acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt
    25081 gcggagctgc gtctctatta ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata
    25141 ggtattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttcagac
    25201 aaacgtgcaa acccaacctg gacgccaacg gcaaacatcc gcgcccgtta taaagcacag
    25261 ggaattgagt tgcctacggt agtgccggct ggaccggata acccaatggg ccatcatgcg
    25321 attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc cgatttcggc
    25381 attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc
    25441 tttagccagg tcaccccagg caccaaagtg aatatcatca acactccgat aaaagtctct
    25501 gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat
    25561 gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca
    25621 gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg
    25681 gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa tcctggataa aaaaaatccc
    25741 cgcgacacgg catgtcgcgg ggaaaaaatc agcagtagca ggcattaatg agggttaatg
    25801 cttatttata aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac
    25861 ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc agcgatccca
    25921 gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct ggcgttgact
    25981 gaacttcgac agccgcaaag ttggcaaatg acatggagct taaaatcgcc gcagcgatga
    26041 gggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg caagaaatta
    26101 cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga tattttttat
    26161 aacaatcatt aattaattta aaaatcttta aatttcagtt aaatagaaag caattatttt
    26221 tatgggatct ggactggtga acggaggtga ttatttttat aataaccttt cattcacagt
    26281 caccaggttc aacggcatca tggcaactga ctcaacacaa tgtgtaaaaa aaagccgtgg
    26341 ccgcccaaaa gtgttcgaca gggatgccgc gcttgataag gccatgaaat tgttctggca
    26401 acacggttat gaagcgactt ctcttgcgga cctcgtcgaa gcgaccggag ccaaagcgcc
    26461 cacgctatac gcggaattta ccaacaaaga ggggttattt cgtgccgttc tcgaccgcta
    26521 tatcgatcgt tttgccgcta agcatgaagc acagctgttt tgtgaagaga aaagcgtgga
    26581 gtctgcgctg gctgactatt ttgctgccat cgccaactgc tttaccagca aagacacccc
    26641 ggcaggctgc ttcatgatca acaactgcac caccctctcc ccagattcag gagatatcgc
    26701 caatacgttg aaatcacgcc atgcgatgca agagcgcact ttgcagcagt ttttatgtca
    26761 acgacaagcg cgcggggaaa tcccgaccca ctgtgacgtg acacatctgg cagaattcct
    26821 taattgtatt attcagggga tgtcgatcag cgcacgcgaa ggtgcatcgc tggaaaaact
    26881 gatgcagatt gccggaacga ctttgcgttt atggcccgaa ctggtgaaat aagtcgcaaa
    26941 aaaagcccct cagcctgagc gaatgagggg ccaaattgca gagtacagcc ttgttatact
    27001 ttgttattca aaatcagttg cccgttacta tctagcggga tctgcgtacc cggatcgcgg
    27061 tccatgcgga ttttgccctg ctgatcgcca atcttatagg tcacatcata accgagcatt
    27121 ttttctgact tgtcatacac cgttttacaa cgctgttgcg tagtcgtgta agtatcgctt
    27181 tcctggagag agccctggat ctggttaccg gcatatccac cacccagcgc ccccacaaca
    27241 gtggcgacat ctttaccacg accaccacca aactgatgcc ctatcacgcc gccagcaaca
    27301 gcgccgagca ccgacccggt aatgcgattt tcatcctgca ccggtcgacg atgggtcact
    27361 gtgacgttgc gacactcctg acgcggtgtt ttaaccgttt ccttgattgg ggttgcagaa
    27421 acaacctgag cgtattgcgg gccccgttca aacacgttca gactggccac tgccgctacg
    27481 cccagcgcag ctgcgacacc aatcccgata cccgccaaca ttgatttatt cacgggaaca
    27541 cctccttctt tgcagttatg ccaatcatgc aagcgaagga gaaaagcagc tatcagacag
    27601 aagatgaaaa gcgcgggaat ggctggaaat acttaaggat tcggagaagt ctgaaggaat
    27661 gcctgatgcg acgcttaacg cgtcttatca ggcctaccgc tcgtaccatt tgtccagggc
    27721 ggatgaggcg tttatgccac atccgccagt gtacgtcgat taatgcaact tcaaacgcgg
    27781 acggataacg cggttaatac tccccaccag catcattaat ccggttttaa agtaaccatg
    27841 cagcgcaatc tgatgcattc ggtatagcga gatatataca aagcgcgcaa ttcgtccttc
    27901 aatcatcatt gagccgcgcg tcaggttacc catcaggcta ccgacagtgg agaagttcga
    27961 cagcgatacc agcgaaccat gatctttata ctgataattt ttcagcggtt taccgttcat
    28021 ctgcgccaga atattgttca ttgcgcaggt cgccatctgg tgtgcagcct gagcacgcgg
    28081 cggaacaaag cccccttccg gacgcgggca tgacgcgcag tcgccaatag cgtaaatgtc
    28141 tggatcgcgg gtggtttgca gcgtcggttc caccaccagc tggttgatac ggttggtttc
    28201 aagaccaccg atatctttca ggaagtctgg cgctttgatc ccggctgccc acaccatcag
    28261 atcagcctca atatattcac catctttagt gtgcaggccg ccttcatcag cactggtgac
    28321 catggtttgc gtcagcacgc gaacgccaag tttcgttagc tcgttgtggg ccgcagcaga
    28381 gatacgtggc ggtaatgcag gcaaaatacg ttctcccgct tctaccagcg ttacgttcag
    28441 ggcttcgttg gtcaggcctt tgtaaccgta gctgtgcagt tgcttgaccg cattgtgcaa
    28501 ttcagcggag agttctacac ccgtcgcgcc gccgccgaca atcgcaatgt tcactttacc
    28561 attcgcgccc aggttggcgg agtatttcag gaacagattc agcatctcct ggtggaagcg
    28621 acgcgcctgg tgcgggttat cgaggaaaat gcagttctct ttgacacctg gcgtattgaa
    28681 atcgttagag gtgctaccca gcgccattac cagggtgtca taggcgattt tacgttccgg
    28741 aaccagcagt tcacctttct catcgcgcag ttctgcaata gtgattgttt tcgcttcacg
    28801 atcaatatca atgacggaac ccagctggaa ctggaaacca tgattgcgcg catgggccag
    28861 atagctcaac gcatcgacgc cttcatcaag cgagccagtc gccacttcgt gcagcagcgg
    28921 tttccacaga tggctgtggt tacgatcgac cagcgtaatt ttggcttttt tcttgcgtcc
    28981 cagcttatgc cccagctgtg ttgccatttc cagcccacca gcaccgccgc cgacaatcac
    29041 aatttttttc aatggcgtag tcaacgtgac ccccttaaat ttattaacca attgttaatt
    29101 aatggttata aacatagctc ttaattaaca acaggttaca gcaatgaaaa tgttcttcgc
    29161 tattgagaat aacatgaatg gtgcattggt cataccaaaa ttgatgttaa tcaagttttg
    29221 ttgctgaaaa gataggcgac tttaccgggt gagatcaaaa aacagagttt attgtttatt
    29281 atttgagcaa gtccggaagg gtgatatagg gtgacgcgta tatcgagggc gtttggtcgt
    29341 cctgataaga tgcgcagcat cgcatcaggc attgatgctc gactgtcgga tgcggcgtaa
    29401 acgccttatc cgacttacag aattcggagc gcatcaggta ccggcaaggt tacctgatgc
    29461 gacgagggca tttacccgag ggttttgaac gccttaatgc gctgcaaatg cggggagata
    29521 ttcttgaatt tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg atgcaactct
    29581 tcagatgtcc gctggctgtt aagcgcttca tcattacgcg acaaaatcac caggcaacga
    29641 tcgcgattct tctcgcggaa gttggtcaca cacttagtgg caatatcggc atactcttcc
    29701 gggcgatcaa tcttgccttc catgttctca taagggaaca aattagggtt gaagatcacc
    29761 tggcggatat cgcaaagaaa accaatccgt tccgcccagt atccgcccag accaacgccg
    29821 caaatcagcg gacgctcgtc gacgttcagt tgcaacattt tgtccacttc tttgagcaga
    29881 tgctgcatat catgtttcgg atgccgcgta ctgtagctta tcaagcgtac atccgggtca
    29941 ataaactgca attgtaagac tttctcgtgg ttacccggac tgttagagtc aaaaccgtgt
    30001 aaatagataa tcatcgcttc ctcacataag ccagggttag tgacctgctt tctcttcctg
    30061 ccagcgttca tgtaactgat tcagacgggt gctgatcgcc ttccagcgag ccgagtccat
    30121 cagttcctgt cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt ctgccttgat
    30181 cggtgacaga ttatctaaca cgctgacggc ccctttacga ttattgcaga ccaggatcat
    30241 atcgcaacct gcgtccagtg atgcctgccc gcgttcggca taactgccca taatcgcggc
    30301 accttccatc gataaatcgt cagagaaaat tacaccgtca aaacacagtt cctgacgcaa
    30361 aacggttttc agccagtagg aagaaccgct cgccggacgc ggatcaacat cactgtagat
    30421 cacatgcgca ggcataatgg cgtcgagttt attttcgcgg attaaggaac tgaagaccga
    30481 catatcttta gcgcgaatct ccgcttgtgg acgtggatcg cacggtgttt ctttgtgtga
    30541 gtctgccgtt actgcaccgt gtcctgggaa gtgtttcccg gtcgttttca ttccggcttc
    30601 atgcatacca tcaataaacc ggctggcaat tgccagggct ttttgtggat cggcatgata
    30661 agaacgctcg ccaatcgccg cgctgatatg cccgacatcc agcactggcg caaagctgat
    30721 atcaatatcc atagcgatca tttcgctggc catcagccaa cccgcctctt gcgccagttt
    30781 gccgccctct tccattccca acagcgcagc aaaggattgt gctgccggta agcgggtaaa
    30841 accttcgcga aaacgctgca cgcgtccacc ttcctgatct accgccacca ccagatgatt
    30901 gcgcgatgct gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat catgatagtt
    30961 acgcgtaaag agaatcagcc ctcccaccag cggatgcgcc agtatttcac gctcttccgc
    31021 gtccagttcg taaccttcga catccaacat tactggaccc acactgctct ccttattgtt
    31081 ttattaatag ctgccgccag gtgtcatcgg ccagcctgat aaattgttga tcgccgattt
    31141 gtcgccagcg gtactcaaac caccctgctt tgagcatcag cagccaggga aaccatcgcc
    31201 tgacctgacg ccataattgc gccggataaa tcttcgcgcg agtggcatag tcattgacca
    31261 attgccggtg ctgttcagta ttttccaccc acaccgccgc cagttccagc gcgatatcac
    31321 catctccggc atactcccag tcgatgagtt ttaaccctga cgcgctatgc actaaatttc
    31381 cggcgtggac atccatatgc aatggactta agcgtaaagg ccgtggttcc cgcgctttgc
    31441 gcagacgttt taacattcgc agccaaccca cagtccgccg cgccggatcg ctttgctgcc
    31501 agtacagttc cagtaagggc aacagcgtta ttcgccagcc aaaacgtggt tgttgatgaa
    31561 gataatacag caagcctgcc agttcgttgg tatccggcaa atacgttttt accgcgccgg
    31621 gcagatagtc gactaccatc cagtcacgga gatataaatg cggcttcggt gcaatgcttg
    31681 cgggtagttg tgataaagcc cgatactggc gcaagaacgc ggactgcggc gcatcaggat
    31741 cgtgcggctg acgcacaaca aaacgctggc cctgatgttc aatgagaaaa ctaccgccac
    31801 taagcccact attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt
    31861 cgtcgcgcgt gatgggatta ttgctgcgaa acggcacctt tacctgacca gataatttcg
    31921 cccgtctgca ccagcatcag ctgcatttgt agggtcggag cgttaacgtt gccagaggcg
    31981 ctggagtaca gcacgtaatg agcgccgaca ttgcgggcaa tgcctatggc tttactacgg
    32041 gtacctaaac tgtcctgcgg cgacaaacct aactgttgct tagccatcga cagctgctgg
    32101 gcggaaacca gggtaaattt cccgttatta gccagcgcat ttcgcagcgt ttcggtcgct
    32161 tctgcggcat tcagcgaacc gttagtacgg ttgttaacgc tatcaaccag caggacgcta
    32221 cccgcagtca ccccgtcagc cccaagcatc ttactgacca tcggctgcat tgcgccattc
    32281 cagtcataat ggcgaatatg cggcgcaggc ggtgccgttt gatcttcgtg ctcaattggg
    32341 cctggctgct gcgggatcgt cggcaccgag ggcactgtgg ggacaggctg ttgtggctcg
    32401 gctggttgtt ccggcgctgg tttcacttct tctaccggtg caggttcacg ttgccccaca
    32461 cacccggcga gaaacatcgc cagcgcggta atcaaggcgt agcgactcat ttttgtcatc
    32521 aagattcacc ccttacaaat atagataaag tctgactttg tgcgccccca gaaaattggc
    32581 gctgccgtac agcgttaccg ccgaatgtgc gggaatgcta acgctgcgtg gcctttccag
    32641 aggatgcatc tccagccctc tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg
    32701 ttcttgcctt tcgttataaa gcgttgagga tgctgaaggt tgaatatcag acgtcgaaag
    32761 gacgggcttt tctgcactga tgcccgcagc caataaagat gactccatca ccagcgattg
    32821 ctcatcattc accggaattt ccggatgtga acgacagccc accagcagta acaacgccag
    32881 agacaccagc ccaaagcatc cttttctcat cgttacagac ctttatgcgc cagcattggt
    32941 cccagcggac ggccacccaa caagtgcata tggatgtggt aaacctcttg tccgccatgg
    33001 cggttggtat tcatgatcag acgatagcca tcttcggcaa taccttcttg ctcagcaatt
    33061 tttgccgcta cggtgatcat gcgtcccagc gcctgctcat gctcagctga cacgtcgttc
    33121 acagtcggaa tgaggatatt cggaatgatc agaatatgcg ttggcgcttg cggcgaaata
    33181 tcgcgaaacg ccgttaccag atcatcctgg tagacgatat cggaggggat ctcacgacga
    33241 ataattttgc tgaatatagt ttcttctgcc acgacatttt cctttttcaa atagccatgc
    33301 gttgcgccat gctacgccgc gccacactgc gagtatagag tatggtcgag ttaaacctgc
    33361 tctttcaacc ttaactcacg atttcttaag caaaaaatcg ttttgctgcc gtatatatcg
    33421 ccattattcc catttctgcc tgcgaatgcg ctgaaaagac attctctgtt tggtttacac
    33481 gacaaatatg aatgcgcata tttctcattt gcatttacaa acaaaattat tcgcacacta
    33541 aatataaccg ctcatcaccc tcctccggca tgagcctgac gacaacataa accaagagat
    33601 ttcaaatgct ttcaacacaa tttaacaggg ataatcaata tcaagccatc accaaaccgt
    33661 cactacttgc cggttgcata gcactggcac tattaccttc tgccgctttt gctgcaccag
    33721 ccactgaaga aacggtgatt gttgagggtt cagccacagc tccagatgat ggcgaaaatg
    33781 attacagcgt aacgtctacc tctgcgggta ccaaaatgca gatgactcaa cttgatattc
    33841 ctcagtcggt cactattgtt agccagcagc ggatggaaga tcagcagtta caaacgctgg
    33901 gcgaagtgat ggaaaacacg ctggggatca gcaaaagtca ggcggattcc gatcgtgctc
    33961 tttattattc ccgtggattc cagatcgata actatatggt tgatggtatc cccacctatt
    34021 ttgaatcgcg ctggaatctg ggcgacgcac tttctgatat ggcactcttt gaacgcgtag
    34081 aagtagtgcg tggcgcgaca ggactcatga ccgggacggg taatccatct gcggcaatta
    34141 atatggttcg aaaacacgcg accagtcgtg aatttaaagg cgatgtctcg gcggaatacg
    34201 gtagctggaa caaagaacgg tatgtggcgg atttacaaag cccactcacc gaagacggta
    34261 aaatccgcgc gcgaattgtc ggcggctacc agaataacga ctcatggctg gaccgctaca
    34321 acagtgaaaa gaccttcttc tcgggcattg tcgatgctga tttaggcgat cttacgacgc
    34381 tttcagccgg ttacgaatat cagcgcattg atgttaatag cccaacctgg ggcggtttac
    34441 cgcgctggaa tactgatggc agcagcaaca gttacgatcg tgcacgcagt accgcacctg
    34501 actgggcgta caacgataaa gagatcaaca aggtctttat gaccctgaag cagcggtttg
    34561 ctgatacctg gcaagcgaca ctgaatgcca cccactctga agtcgaattt gacagcaaaa
    34621 tgatgtatgt cgatgcctat gtaaacaaag cggatggtat gctggttggg ccatacagta
    34681 attatggacc tggctttgat tatgtcggcg gcaccggttg gaacagtggc aaacgtaaag
    34741 ttgatgcgct ggatttgttc gctgacggta gttatgaatt gtttggtcgt cagcacaatc
    34801 tagtgtttgg tggcagttac agcaaacaaa acaatcgtta cttcagttca tgggccaaca
    34861 tcttcccgga tgaaattggc agtttctaca actttaatgg caatttccca caaaccgact
    34921 ggtcaccaca gagcctggca caggacgata ccacacatat gaaatcgtta tatgctgcta
    34981 ctcgagtcac ccttgccgat ccgctgcatc tgatcctcgg cgcacgttat accaactggc
    35041 gggttgatac gctgacttac agcatggaga aaaaccacac cacgccttac gctggtctgg
    35101 tgtttgacat caatgacaac tggtcgacct acgccagcta tacctctatt ttccagccgc
    35161 aaaatgatcg tgacagttca ggcaaatatc tggctccaat caccggtaac aactacgagc
    35221 tgggtctgaa atcggactgg atgaatagcc gtctgaccac cacgttagcc atcttccgta
    35281 ttgagcagga taatgtcgct cagtccaccg gtacacctat ccccggcagc aacggcgaaa
    35341 ccgcctataa agcggtggat gggacagtca gtaaaggggt ggaatttgaa ctcaacggcg
    35401 caattaccga caactggcag ctgacatttg gcgcaacgcg ctatattgca gaggataacg
    35461 aaggaaacgc cgttaatcct aatctgccac gcaccacggt taaaatgttc accagctatc
    35521 ggttgcctgt catgccagag ttgacagtcg gcggtggtgt taactggcaa aatcgcgtgt
    35581 ataccgacac cgtgacaccg tatggcacct tccgcgccga gcaaggtagc tacgcgctgg
    35641 tggatctctt cacccgctac caggtgacga aaaacttctc gttacagggg aacgtcaata
    35701 acctgttcga caaaacctac gataccaaca tggaaggttc tatcgtctac ggcgcaccgc
    35761 gtaatttcag cattaccggc acgtatcaat tctgaataac acctgtaaaa aaggcagcca
    35821 tctggcggcc ttagtctccc caacgtctta cggattagtg gttacggatg tactcatcca
    35881 tctcggtttt caggttatcg gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa
    35941 ccactacgcc cgctgcgccc agtttcttca ggccagcctg atccacttta gacacatcag
    36001 caacgctgac gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt tctttaccac
    36061 caaatgcagc aaccagagcc ggtgccattt cgctggtacc tgttgctttt gcatcttcag
    36121 tcgcgtcttc acgacccggc gttttcagat ccagtgcttt aatcagcacg cggaagatgg
    36181 tgtagtaaac aatcgcataa ccgataccga cgatcgggaa cagccacagt ttgctgctgt
    36241 taccagacag aacgataaag tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc
    36301 ccagaagaat acagattggg aatgccaggc ctgccagaat cgcgtggatg atgtacagga
    36361 tcggcgcaac gaacatgaag gagaactcga tcggctcggt gataccggtc aggaacgagg
    36421 tcagcgccgc ggagatcata ataccgccca ctttcgcgcg gttttctggt ttagcagagt
    36481 gccagatagc aattgcggca gctggcagac cgtacatttt gaacaggaag ccaccagaca
    36541 gtttacccgc agtcgggtca cccgccatat aacgcggaat gtcgccgtgg aaaacctgac
    36601 ctgctgcgtt ggtgtattca ccaatctgca tctggaaagg tacgttccag atgtggtgca
    36661 gaccaaacgg caccaggcaa cgttcgatga aaccgtaaat gccaaacgca actaccgggt
    36721 tctggtaagc agcccactga gagaaggtct ggattgcaga accaatcggc ggccaaatga
    36781 aggacagcac aacgccagta aagatggcag ccaggccaga aatgatcggc acaaagcgtt
    36841 taccggcaaa gaagccaaga tactcaggca gcttaatacg gtagaaacgg ttaaacatgt
    36901 acgctgcgat cgcaccggag ataatccctc cgagtacgcc agtatccgcc aggtgtttag
    36961 aggcgatttc ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc atggttttaa
    37021 ccatgatgcc ataggcaaca actgcggcca gcgcggatac gccatcgtta ttggtaaagc
    37081 cgagggcgac accgatcgca aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt
    37141 ctgccataac atgcgataca acggcgggca gccagctgaa attcgcggaa ccgacgccca
    37201 gcagaatacc tgcgataggc agtacggata ccggcagcat cagcgattta ccgacctttt
    37261 gcaggttagc aaatgcattc ttaaacataa ttgagagtgc tcctgagtat gggtgctttt
    37321 tttacgttct cacgcgtggc aaggggggag agcctcgccg tgtacagggc atctaagcgc
    37381 cctttattta ttacacagag taaaataatt cagtgccgat ttgtttgacg gctatcacgt
    37441 ttcaacttca gatcattgga gaagttgcac attttgacaa aacatatgtc acaatatttc
    37501 aaacgtggtt attcaccgcc caattttcgg cggtgaactt tactcgtttt agccattaat
    37561 tacgagcttt aaaaaaactc attcaacgga tggattgaag gcgggaagcg tcgatgtgaa
    37621 acagacgggc gaagttatcg gtggttacct gcgccagttc ttcaacggca acacctttca
    37681 acacagccat gtattctgca acgtcacgaa ccatcgcagg ttgattctct tttcctcgat
    37741 gcggtaccgg cgcaaggtaa ggtgagtcag tttccaccag taaccgatcc agggggacat
    37801 aacgcgcagc atctcgaagt tgctccgcat tacggaaggt cacaatgccg gaaaaggaga
    37861 tgtaaaatcc gagatccagt aatttacccg ccgtttctct gtcctctgta aaacagtgta
    37921 gtacgccacc gcaatccgtc actttttctt cgcgcagaat tgccagcgta tcggcgcggg
    37981 cgtcacgggt atggacgata accggcttgt tcagttcacg accaatctgg atatgatgga
    38041 tgaaggactc ttgctgacga actttagttt ccggtgtgta ataataatcc agcccggttt
    38101 cacccagcgc tacaacaccc tcttctgccg ccagacggcg taaatcttct acatcgtagg
    38161 gatcattctg gttgagtgga tgcacgccac aagaaaatac gacgttgtca cgttcgccta
    38221 ccagatcccg catatgtaaa taacccggta atgttgtggc gactgccaga caaaatttca
    38281 catcgcgtgc ggcggctttc gccagaacgt catccacgtc cttatgcaaa gattcataat
    38341 ccagaccatc gagatggcag tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga
    38401 ggaaccggta gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa aagatcggtg
    38461 atgagaagct cgcggttgat gcctgtgaca gacattaact gttcacgaat gtggcaaaca
    38521 tcccccagta tagcctgcag gcgcgaggga gaaagatggt ttgccagttc gacgacaagg
    38581 cccggcacat caacattggt cacctgcgca gcaccatgat ggcgttttag cgcatccatc
    38641 agcaacgttg ccagccagtg taaacgcgcc ggagcttgtt catgattaag ggccgccagc
    38701 agcgaatacc agtcgcccga tggcacgcta tatgccaatg cctgacacaa tgtttcacga
    38761 gcctgccagt tatctccctg aaacaacgcc agtgccgcgc caggcgaacc ggcgcttaag
    38821 cgcaatgcgg caagtaatgc atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg
    38881 gcgtactgtt ccggcggcgg cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc
    38941 agtaaacgtt caggctcgcg ggtagccagg aaaaaccagg tttctgctgg tggctcttca
    39001 agcgttttca gcaatgcgtt agccgcggcg tcggtcagta aggcagcatc ggttacccag
    39061 acaactttcg caccacctag gcgtgcgtgc tcattcagct tttcggtgac ctcacgtact
    39121 gcatcaatgc ccagcgtatt tttccctttt tcgggagcca gggtgtagta atcgggatgc
    39181 gtgccagcct gcatcaactg acatccacga cagtgaccgc aacttttgtg gccctgcggt
    39241 tgttggcaga gtaaataacg gctcagggcg tagattaaag catcatcgcc catgcccggt
    39301 aacgcctgaa tgagtagcgc atggtgacct cttccggcct gatagctggc taccagtttt
    39361 tcgaaatcag gtcgtaacca tggataccat ctcatgcgtc caactccttc acccagtggg
    39421 tcacggtagt gcggattgca tccatcacgg cctccagcgg ctgggtggca tcaatggtat
    39481 gaatactttt atcttgtgca gccagttcca gatagcgggc gcgggtgcga ttaaagaaat
    39541 cgaaagactc ttgctcaata cgatccagct cgccgcgcgc acgcgcgcgt tttaagccaa
    39601 cttccggggt aacatcgaga tagagcgtta agtcggggcg aaaatccccg agaacagcat
    39661 cacgcagtgt tgccagcata tgttggtcaa taccacgtcc tccgccctga tacgcctgag
    39721 tggagagatc gtggcgatcg ccaatcaccc aggtgccgtt agccagagct ggtttgatga
    39781 ccgtttctac cagttgaacg cgcgcggcat aaaacatcag aacttcggct ttatcggtaa
    39841 tgacttcatc gcctaccgat ttgatatcca gcaccaggct tcttaacttt tcggcaagtt
    39901 gcgtaccgcc aggttcccga gtgaaaacca tgtcgcggat acccagttgc tcaagcgtct
    39961 caaccaccac attacgcgcg gtagttttgc ctgcgccttc cagcccctca atgacgatat
    40021 acttactgcg catttttttc cttaagcact ttcagataat cctgcacaga cttgttatgg
    40081 ctggcaagat tggtattaaa cgtgtgacca cctttaccat cggccacaaa atagagatac
    40141 ggcgtttttg ccggatgcgc agcagccttc agcgaatccg cccccggcgt agctatcgca
    40201 cccggcggca aaccggtaat ggtataggtg ttatacgctg tcggcgtttc caggtctgca
    40261 cgagaaagtt tgccattata acgctctccc atcccgtaaa tcacggttgg gtcggtctgc
    40321 aggcgcatac caatgcgtaa acggttgata aataccgagg caacctgatc gcgttcactg
    40381 gcaacggcgg tttctttttc gataattgat gccatcgtca ccagctgatt tttatcttta
    40441 taaggcagac cgtccgcacg cccttcccag gcgctatcga ccgctttcac cattttcttg
    40501 tgcgctcgct tgagtaacgc gacatcggtg gtattggcgg tatacatcca ggtgtctggc
    40561 cagaaccaac cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg
    40621 tacttatcgt cactcagcgt gtgcttgata tacggagcct cacgcaattg cttgaggtag
    40681 tcgctcagac gcatcccttc taccagtcgc agcgggaact gtgcttcttt accgctttcc
    40741 agcaatttca gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt cccggcttta
    40801 aagtgagaaa gatccggttc gatacgcagc aaccattgaa aaacccgtgg gcgattgatg
    40861 atcttatcgg cataaagctg ttcaccaagc gccagacgtc cggtccctgg cttcagggta
    40921 aatatcgtct cttctttgat aagcaatttg ctgtcggcaa gatggcgaac cttccagacg
    40981 cccacaccag cggcgatacc cagtaccacc agcaataaca agataatcaa taacactttt
    41041 ttcatgacta attcgggcgc tcacaaagtg gggctaaata ttcatataac gttgctgacg
    41101 aaaaggagac atcgccacag gcacgtacgg gcatcactgg cattaacgca ttacaaataa
    41161 ccatctcatc tgcctgcaac gcctcttcca gagaggcttg cacttcgaca agctgataag
    41221 aggattgtgc cagcaaacgg atacagaatt gtcgcataat gccgttaaca cctgcctgat
    41281 ccagacgcgg cgtataaact acattgccct tccgccagaa caaattagcc gcacagcatt
    41341 ccgtaaccca cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat
    41401 gagagcggat caatacttgc tcaagccgat taagatgttt aatacctgca agatgaggat
    41461 tgcgccccag ccgcaccggg cttagcgcca acgtcatccc ttcgttacgc aaacggtcgt
    41521 aatgtgcagg ataaaccgta acggagagaa tccgcgttgc cggtccactg ttcaatgtgc
    41581 tgtaccctcg cccgccacta ccgcgactga tcacgacttt cagcacacca ttttgctgtt
    41641 ctgctgccag cgttttcatc tcttgttcaa gctgaggcca gaagtcacag aaaatcatca
    41701 accgctgaca agcatcctgt agtcgctgga tatgcgccga taacaaactg actttaccgt
    41761 cgataactct ggcggtggta aaacaaccat caccaaactg cgttgcccga tcgcttactg
    41821 ccagcgattc ctgcttatga ccgttaatta agaacatacc ggctccttat cagagaattg
    41881 ttagtgtggc agcatgttca ctacggaaca agtcggaata aaagctaaga aaaaaggccc
    41941 gcaagcggac cttttatatg ggtgggaaat gacaacttag atctttttaa agatcaaaga
    42001 accattagtg ccaccgaagc cgaaggagtt acacagcgtg tattccattc cgctgacctg
    42061 acgtgcttca tgcggtacga aatccagatc gcaaccttca tccgggttat ccaggttgat
    42121 ggttggcgga acagcctgat cgcgcagcgc caggatggag tagatagatt ctactgcacc
    42181 cgccgcacct aacaggtgac cggtcataga tttcgtggag cttaccaaca cacggcttgc
    42241 agcttcaccg aagatggttt tcaccgcctg cgcttcagct ttatcgccag ccggcgtaga
    42301 agtaccgtgc gcgttaacgt agccaatctg actcgcttca atgcctgcat cacgcagagc
    42361 atttgccatc gccagagctg cgcctgcgcc attttctggc ggtgacgtca tatgataagc
    42421 atcgctgctc ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc
    42481 gtgttcgtac tcttcaagta ccagcatacc ggcaccatcg cccagtacga agccatcacg
    42541 ctctttatcc cacgggcggc tcgccgcttg cgggttatca ttgcgggtag ataatgcacg
    42601 tgccgcgcca aaaccaccaa cacccagcgg cgtactggct ttctctgcgc cacctgcaac
    42661 catcacgtca gcatcgccat acgcgataat acgcgcagca tggccaatgt tgtgcacgcc
    42721 ggaagtacag gcggtcgcga tagagatgct tgggccacgc aggccataca tgatagtcag
    42781 atgacctgcc accatgttca caatcgttga cggaacgaag aatgggctga tcttacgtgg
    42841 accaccgttc atcagagatg tgtggttttc ttcgatcagt ccgaggccgc caatcccgga
    42901 gccaattgcg gcaccaatgc gggttgcgtt ctcttccgtt atatcaaggc cagaatcctg
    42961 catggcctga acgccagcga caattccata ttgaatgaag gcatccatct tgcgctgttc
    43021 tttgcgcgag ataatgtcct cacagttaaa atcctttact aagccagcaa atttcgttgc
    43081 ataggcgcta gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg caagcagagc
    43141 tttccaggta gactctacgg tattgccgac aggagacaac atgcccagtc cggtcacaac
    43201 tacacgacgc ttagacacgt ttgtcctcca gggagggaaa aaatgattct agtgggacaa
    43261 aaagataaaa ctcaggcggt cgaacgaccg cctggagatg ttcacttacg cctggtggcc
    43321 gttgatgtaa tcaatggcag cctgaacggt ggtgattttc tcagcttctt cgtccggaat
    43381 ctcagtatca aactcttctt ccagagccat taccagctca acggtgtcaa gagaatccgc
    43441 gcccaggtct tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa cgcccagctg
    43501 ttcgccgata attttcttaa cgcgttcttc gatagtgctc atactcttaa atttcctatc
    43561 aaaactcgct ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa
    43621 tcccggcagg tcttaccacg attttacgtt attttgaggc cttacccccc aataacgcaa
    43681 atcattttgc actaattatt actcataacc acgcagactg cgcgcaacat gagcattttg
    43741 tgcaaatcgc ggtcagacca tgtacatccc gccgttcaca tgcaaagttt cacccgtgat
    43801 gtaagctgct tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc
    43861 gaggcgaccc gcaggaacct gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg
    43921 tgtcatgtcc gtttcaataa agcccggagc aacaacgttt acagtaatac cgcgtgacgc
    43981 aacttcgcgc gccagtgatt tactgaagcc gatcaagccc gctttcgccg cagcgtagtt
    44041 ggcctgaccg ccatttccca tggtaccaac cacagaaccg atagtgataa tacgaccatg
    44101 acgctttttc atcatagcgc gcattaccgc ttttgacaga cggaaaacag atgaaaggtt
    44161 ggtttcgata atatcgttcc actcttcatc tttcattcgc attaacaggt tatcacgagt
    44221 gataccggca ttattgacca ggatatccac ttcaccaaat tctgcgcgaa ttttttccag
    44281 aacagattcg atagatgccg ggtcggtcac attcaacatc agacctttgc cgttggcacc
    44341 taaataatcg ctgatcgcct gagcgccatt ttcactggtc gcagtgccaa taactttcgc
    44401 gccacgggct gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg caccggttac
    44461 cagtgcgatt tttccttcaa aattcatgat tttcctcttt taaagctcaa gcgccgctgc
    44521 catcgctgaa ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag
    44581 gccagtaagc accttgcccg ggccgacttc atagagatgt tctacgcctt gcgctgccat
    44641 gtactcaaca gacttcgtcc actgaaccgg gttatacaac tgacgtacca gtgcgtcacg
    44701 gatggcatca ccattggttt cgcatttcac atcaacgtta ttcacaacag aaactgttgg
    44761 tgcgttaaag gtgattttcg ctaattctac tgccagtttg tcggctgctg gtttcatcag
    44821 cgcacagtga gacggtacgc tcactggtaa cggcagagca cgttttgcgc ccgcagcttt
    44881 acaggcagcg ccagcacgct caaccgcttc tttatgaccg gcaataacca cctgtcccgg
    44941 agagttaaag tttaccggag aaacgacctg acctgctgca gcttcttcac acgctttcgc
    45001 aatagacgca tcatccagac cgatgattgc cgccatagcg cccgtacctt ccggtacggc
    45061 ttcttgcatg agcttgccgc gcatctcaac cagacgcacc gcatcagcga aatcaatcac
    45121 accagcgcaa accagcgcgg aatattcccc caggctgtga ccggccatca ttgccggtgc
    45181 tttaccgccc tgctgctgcc atacgcggta cagcgcaaca gaggcagtca acagcgctgg
    45241 ctgggtttgc caggttttat tcagttcttc agctggcccc tgctgggtca gcgcccacag
    45301 gtcgtagccc agcgccgcag aagcttcagc aaacgtttct tcgacaattg gatagctcgc
    45361 cgccatatca gccagcattc caacggtttg agaaccctgt ccagggaaca caaatgcaaa
    45421 ttgcgtcatg ttttaatcct tatcctagaa acgaaccagc gcggagcccc aggtgaatcc
    45481 accgccaaag gcttcaagca gaaccaactg ccccggctta atgcgcccgt cgcgtacagc
    45541 ttcatccagc gcgcacggga cagaggccgc agaggtatta ccgtggcgat ccagcgtcac
    45601 cacgacattg tccatagaca taccgagttt tttcgccgtt gcactgataa tacgcaggtt
    45661 agcctgatgc ggaaccagcc agtccagttg agaacggtca agattatttg ccgccagcgt
    45721 ctcatcaacg atgtgcgcca gttccgttac cgcaaccttg aagacttcgt tgcccgccat
    45781 cgtcagatga attgaattct ctggattcac acggtcagca ttaggcagcg tcagcaactc
    45841 accatagcta ccgtcggcat gcagatgggt ggagatgatt cccggctctt cagaggcagc
    45901 cagcaccgca gcgcccgcgc catcgccaaa aataataata gtcccacgat cggttggatc
    45961 gcaggtgcgc gccagtacat cggaaccgac gaccagagca tacttcaccg ccccagattt
    46021 cacgtattga tcggctacgc ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc
    46081 aaatgccggg caacctttaa tgcccagcat gctttgaatc tgacaagctg cgctcgggaa
    46141 agcgtgcgta gcagaagtcg ttgccacaac gatcaggcca atctggtctt tctcaatgcc
    46201 cgccatctca attgcgcgtg tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg
    46261 cgcggcaatg tggcgttcgc ggataccggt acgagtgaca atccactcgt cagaggtgtc
    46321 caccattttt tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc tgccagtacc
    46381 aataatcttc gtatacatgt acgctcagtc acttttcggt tatataccgt cacttgcaaa
    46441 ctgcgagttc gctggcagcg tcctgctacc gcagagttcc gcttttgcca ccgtccagca
    46501 gctcaaaacc agctgggtat acagattcca ggcgagcggc aattcgctga ggaacttgtc
    46561 gctgcaccgc ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac
    46621 catgactttt tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg
    46681 ggttgaggtg actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt aataacagta
    46741 gccaccacga ccgttttttc ccttcaccct gagatttcag cagagaaagg aacatcctga
    46801 caacaccttc catcgtcttt aatgtgacat ttcctgtaaa gccgtcacaa accagcacat
    46861 ctgtcttgcc agttaacaac tcattggctt caagatagcc gatataattg atagaaggga
    46921 ttgttttaag caccgctgag gcatcccgaa tactgtcgag accctttact tcttcttcac
    46981 caatattgag caacgccacg cgaggattgg gaatttccac cacctcttca gccagaactg
    47041 agcccataat ggcaaattgc accaacattg tactgtcaca atcgacgttg gcccctaagt
    47101 caaggaccac cgttttgccc ttttgctgat gtggtaatac cgtcaccagc gccggacgct
    47161 caatcccctc caggggcttg agtaataatt ttgccagccc catcagtgcc ccggtattac
    47221 cggcactgac acaggcttgc gctcgacctt ctttcaccag ctccagggcc acgcgcattg
    47281 aactcccacg actggcgcgg atagcttgcg aaggccgggc atcactggcg ataactgact
    47341 gcgcaggaat aatctgcaga cgcgaacgtt gttcaaagtc agctttagca agtaatggcg
    47401 tgatggcgtc gggattgccg actaaaagaa gagtgagttg cgaattagaa ttcagtgcct
    47461 gcaatgctgc aggcactgtc acggaagggc caaaatcccc tcccatgaca tctaacgcca
    47521 gggttagacg tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg
    47581 aaaatcctca ctaagcttca tcacgcagac gcgtgattac ttagcgatga ccttgcggcc
    47641 gcggtagtaa ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc
    47701 tacagacagg ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga
    47761 acgggttggt ttattctgtt gtacggccat ggaccttact cctcaattac ggtttatccc
    47821 cgatccaacg gggagcacca attacttacg ctttaagctg gctaatacgg caaatgggtt
    47881 tggcttttgc acttcttcag gcagttcacc aaagaccatg tccgcttcgg acacttcaca
    47941 gtgttcagaa tcatgcaccg gaactaccgg caaggcgagg atgatttcat cttcaaccat
    48001 tgcaagcaga tcgatttcac cgaattcgtt aacctcaatc ggttcatacg cttccggcag
    48061 tgcttcagcc tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt agacctgatg
    48121 agtaaacggc ttcccgcaac gctgacactc gagcgttacc gtcaccttcg catcgccgtt
    48181 taacactgcg agacgttggt tatcgatagc gaacgacatg gagcattcca catcactgtc
    48241 cacactgact acggattcgg cgacgcgctc aacctgatca ggggtataga taccctggta
    48301 atcaaggcgt ttttgagccg tacgaaccgg atcgagagtc aggggtaatt ttaccttttg
    48361 catagggcgc gcatattaac tttgtaacgt catagagtca aagaaaaagg cagcctgtgg
    48421 ttgccttttg ccaataattc gcacacattg cgggttactg ctttattttc gttcagtgct
    48481 gataacgaac atcaacctgc aaaacgaagc gcatagttta aaatggctat catcaatacg
    48541 ctatatctga tggaaaaaaa tatgcctaaa cttattttag cctccacctc gccatggcgt
    48601 cgcgctcttc tggaaaaact gcaaatctct tttgaatgtg cagcaccaga ggtcgacgaa
    48661 accccacgca gcgacgaatc accgcgacag ttggtgcttc gactggcaca agaaaaagcg
    48721 caatctctgg cgtcacgtta tccggatcat ttaattattg gttcagacca ggtatgtgtt
    48781 cttgatggcg aaatcaccgg taaaccgtta acggaagaaa atgcctgtct gcaattacgc
    48841 aaagccagcg gcaatatcgt caccttctat actggactgg cgttgtttaa ttcggcgaat
    48901 gggcatctac aaacagaagt ggagcctttt gacgtccatt tccgtcacct gagcgaggcg
    48961 gagattgata attacgtgcg taaggagcat cccctgcact gcgcgggtag ctttaagagt
    49021 gaaggatttg gcattacgct gtttgagcgt ttagagggac gtgaccctaa cacgctggtt
    49081 ggtttaccgc taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa cccgctgatg
    49141 ggatagttgt ttgcgctgga tgcggttacg tcgcatccgg caatcggtgc aggcggcctg
    49201 atgcggcgta aacgccttat caggccaacc gcccattatc aggcttatat gcttgtatta
    49261 gcgcgcgtta cgcagctttt gcaaacaacg cttcaaacct tcatccatcg gcgcttcgat
    49321 acgcatcacc tcaccggtcc ccggatgggt aaacttcaac gctgcggcgt gcaggaacag
    49381 acgatttaat cccgtgcctg cttcagtgag ctgtctgtca aattcacggt caccgtagcg
    49441 atcgtcaaag gcaatcggat gacccgcata ctgtgtatgc acacggatct gatgagtacg
    49501 tccggttacc gggctacaac gcaccagggt ggcaaatgca tagcgttctt ccactttaaa
    49561 gcgtgtttcc gacggtttgc cttcctgact gacacgcacg atacgttcgc cgctttgcag
    49621 aatatttttc aataacggcg cttgaacgct cttcacatgc gactgccact gaccgcgcac
    49681 cagcgccagg taatcttttt gcattccttt ttcacgtaat tgctcatgca gagaacgcaa
    49741 cgccgagcgt tttttcgcta tcagcaacac acctgaagtg tcccggtcga gacgatgaac
    49801 cagttcaagg aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcgataa cgccgaagct
    49861 taaaccactg ccgccatgta ccgccgtacc ggaaggttta ttcagcacca ggatatgatc
    49921 atcttcatat aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc
    49981 ctcttcttcc cgctcagcaa cgcgaaccgg tgggatacgc acctcatcac ccgcttcgag
    50041 tttatattca ggcttaatac gttttttgtt cacccgcact tcgcctttac gcaaaatacg
    50101 gtaaatcata ctttttggta cgcctttcaa ttgggtacgc aaaaagttat cgatacgttg
    50161 ccccgcttcg tcagcggtga tagcaacaat ttttacggat ggagtctctg ttttcatggt
    50221 gggcgattct aattagccaa caggattcgc gccactcatt tttctatgct tatatttagt
    50281 tttgcacctt attacttcac tacgtgatca ctttattgat ggttattaaa ccaatcacca
    50341 gcaagaagtg aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca tcttgctata
    50401 acaaggcttg cagtggaata atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa
    50461 ttttacggaa taacccattt tgcccgaccg atcatccacg cagcaatggc gtaagacgta
    50521 ttgatctttc aggcagttag cgggctgcgg gttgcagtcc ttaccggtag atggaaatat
    50581 ttctggagag taatacccag tctgtttctt tgataattgc gctgtttttc cgcatgaaaa
    50641 acgggcaacc gacactctgc gcctctttga gctgacgata accgtgaggt tggcgacgcg
    50701 actagacacg aggccatcgg ttcacacccg gaaaggcgtt actttgcccg cagcttagtc
    50761 gtcaatgtaa gaataatgag taagttacga tgaaaagaat gttaatcaac gcaactcagc
    50821 aggaagagtt gcgcgttgcc cttgtagatg ggcagcgtct ttatgacctg gatatcgaaa
    50881 gtccagggca cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc cgcattgaac
    50941 cgagtctgga agctgctttt gttgattacg gcgctgaacg tcacggtttc ctcccactaa
    51001 aagaaattgc ccgcgaatat ttccctgcta actacagtgc tcatggtcgt cccaacatta
    51061 aagatgtgtt gcgtgaaggt caggaagtca ttgttcagat cgataaagaa gagcgcggca
    51121 acaaaggcgc ggcattaacc acctttatca gtctggcggg tagctatctg gttctgatgc
    51181 caaataaccc gcgcgcaggt ggcatttctc gccgtatcga aggcgatgac cgtaccgaat
    51241 taaaagaagc actggcaagc cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg
    51301 ctggcgtagg caaatctgct gaggcgctgc aatgggattt aagcttccgc ctgaaacact
    51361 gggaagccat caaaaaagcc gctgaaagcc gcccggctcc gttcctgatt catcaggaga
    51421 gcaacgtaat cgttcgcgca ttccgcgatt acttacgtca ggacatcggc gaaatcctta
    51481 tcgataaccc gaaagtgctc gaactggcac gtcagcatat cgctgcatta ggtcgcccgg
    51541 atttcagcag caaaatcaaa ctgtataccg gcgagatccc gctgttcagc cactaccaga
    51601 ttgagtcgca gatcgagtcc gccttccagc gtgaagtccg tctgccgtcc ggtggttcga
    51661 ttgttatcga cagcaccgaa gcgttaacgg ccatcgacat caactccgca cgcgcgaccc
    51721 gcggcggcga tatcgaagaa accgcgttta acaccaacct cgaagctgcc gatgagattg
    51781 ctcgtcagct gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc atcgacatga
    51841 cgcctgtacg ccaccagcgt gcggtagaaa accgtctgcg tgaagcggtg cgtcaggacc
    51901 gtgcgcgtat tcaaatcagc catatttctc gctttggcct gctggaaatg tcccgccagc
    51961 gcctgagccc atcactgggt gaatccagcc atcacgtctg cccgcgctgc tccggtaccg
    52021 gtaccgtgcg tgacaacgaa tcgctgtcgc tctctattct gcgtttgatc gaagaagaag
    52081 cgctgaaaga gaacacccag gaagttcacg ccattgttcc tgtgccaatc gcttcttatc
    52141 tgctgaatga aaaacgttct gcggtgaatg cgattgaaac gcgtcaggac ggtgttcgct
    52201 gcgtgattgt gccaaacgat cagatggaaa ccccgcacta ccacgtgttg cgcgtgcgca
    52261 aaggggaaga aacgccgacc ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg
    52321 cgctgccgtc tgaagaagag ttcgctgaac gtaagcgtcc tgaacaacct gcactggcaa
    52381 cctttgccat gccggatgtg ccgccagcgc caaccccagc tgaacctgcc gcgactgtcg
    52441 tagccccagc accgaaagct gcaacggcaa caccagcagc tcctgcacaa cctgggctgt
    52501 taagccgctt cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa gccaaaccga
    52561 ccgagcaacc aacaccgaaa gcagaagcga aaccggaacg tcaacaggat cgtcgcaaac
    52621 ctcgtcagag caaccgccgt gaccgtaatg agcgccgcga cacccgtagt gaacgtactg
    52681 aaggcagcga taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag cagcagactg
    52741 ccgagacgcg tgagagccgt cagcaggctg aggtaacgga aaaagcacgt accaccgacg
    52801 agcagcaagc gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc
    52861 aacaagaagc gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc gaacaggaag
    52921 aacgtgtacg tccggttcag ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt
    52981 acgagcaaag cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa actgccgctg
    53041 ccgaaccaat tgttcaggaa gctccagctc cacgcacaga actggtgaaa gtcccgctgc
    53101 cagtcgtagc gcaaactgca ccagaacagc aagaagagaa caatgctgat aaccgtgaca
    53161 acggtggcat gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgtg agtggtcagc
    53221 gtcgtcgtcg ctatcgtgac gagcgttatc caacccagtc gccaatgccg ttgaccgtag
    53281 cgtgcgcgtc tccggaactg gcctctggca aagtctggat ccgctatcca attgtacgtc
    53341 cgcaagatgt acaagttgaa gagcagcgcg aacaggaaga agtacaagtg cagccgatgg
    53401 tgactggggt ccctgtcgcc gccgctgtcg aacctgttgt tagcgcaccg gttgttgaag
    53461 aagtggctga agttgtagaa gcccccgttc agattgccga accgcaaccg gaagtggttg
    53521 aaacgacgca tcctgaagtg attgccgccg cagtaactga acagccgcag gtgattaccg
    53581 agtctgatgt tgccgtagcc caggaagttg cagaacacgc agaaccggtg gttgaaccgc
    53641 aggaagagac ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac
    53701 ctgaagttgt tgctcaacct gccgcgccag tcgtcgctga agtcgcagca gaagttgaaa
    53761 cggtagctgc ggttgaacct gaggtcaccg ttgagcataa ccacgctacg gtgccgatga
    53821 cgcgcgctcc ggcaccggaa tatgttccgg atgcaccgcg tcacagtgac tggcagcgcc
    53881 ctacttttgc cttcgaaggt aaaggtgccg caggtggtca tacggcaaca catcatgcct
    53941 ctgccgctcc tgcgcgtccg caacctgttg agtaataatt agctcaagta atcaagccct
    54001 ggtaactgcc agggcttttt tatttcatct ttgaatcaac atattaaaat tcatcgaatg
    54061 gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaat gcccgccccg gcgatcccag
    54121 agcaggcaga caaaacatac ccagtttcat gatatgtttt aaagcgaaat tatttgttga
    54181 gctggaagag cgacaatccc tgcatatcgg taaatgcttt atacgatgcc tgcaatgccg
    54241 tttgctgcat gatgtaagat gaaatagttg cattccagtc cacatcaacc agatcgctca
    54301 tctgctgcgt ttgccctaaa gcgcgatcgc tacctaatga atccagcgac tccagttcgt
    54361 tcagctgcgt gcctaattcc gcgcgaacgg tcagcacatt gttcagcgag tttttcagtc
    54421 cgcggttggt tttatctaac gccgcagcgg cggtttcttt atccgcttcg ctatccgcga
    54481 ccggcgtttt cagggctgcg atggcgctat ccagcatggc aaaaagattg gtttcagaag
    54541 cgctaccgtc tggttccgct accgcgttac tggtaatact gtcgaaaatt ttgtcgcccg
    54601 tatgtcctat caccatcgaa cgcgaagcat cgacctgttg cttaatactt tctgttccgc
    54661 cgacataatc accgttagcc tcactaaacg gcgcagtctc tgttttataa ccggcaaaaa
    54721 tgtagcgccc attaccgtca gtggtgttcg ccagattcag caactggtca cgaagcccct
    54781 gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga
    54841 caattttttc ctgagcattc tggatagcag tggtgacctg gctaagtaca ctctcttcca
    54901 gtgacacttt ttgagtggcg aaagtacgcg ccagcgtgta ctggctgttt tgcgcctgtg
    54961 cctgggagag aacaaccgct tgtgatgcag caatgggatc atctgaaggg ttaacgactc
    55021 gcttacccgt cgacatctgt tcgccgtact tcatccattc tgcctgagaa ttggtgatac
    55081 cacgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct ccccttagcg
    55141 aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag
    55201 gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga caccggaaat
    55261 cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc tactggtttt
    55321 caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt cgttaaagga
    55381 tttcgcaccg cccaccgttt tactgttgct ttgcagatcc agcagggcct gaccgttgcg
    55441 gttatcgcta tcacccgcat ctttttcgct cgccatcgct attttcgctt cgtcggtgat
    55501 taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc tgtcgttaac
    55561 ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac cgttggcatc
    55621 cggcgtcacc gtaaaagtgg tattgctggc aaggcgggtg acctgccact gattattatc
    55681 gaacgagatt ttgtaatctg tcgccagtac cacggaggca tcagttaccg tggcaccgat
    55741 cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga tagtaaagaa
    55801 atcttcacca gcatcgccgt tagcatcaaa tccggctttg tgttgggtgt tgaaagcctc
    55861 ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagct cctgagaacg
    55921 gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga tctcaatatt
    55981 gcctgccgtc ccatcaacat aagcgacagt cgtacgagaa gggtcagcgc tggaaggaac
    56041 tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg ccaccgtgat
    56101 gttataagtg ccgccatcct gaacgctgac ttctacacca acaatctggt ttaattcgct
    56161 caccagttga tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt
    56221 caggcgcgag atttgatcgt tcaggctggc aatttgttta gcgtaattgt tgatctgatc
    56281 aacgctggca cctatcgcga tattgacctg tttgtcctgg tcgcgcagat attgatcggt
    56341 ggttttaaac tgattcacca acccttctga tttcccaatc agcgcctggc gcgctgccgg
    56401 gtcttccgcg ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc
    56461 cagcgaagag gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc
    56521 ggtcaggccg ctactttgcg tctgcgcagc acgtaactgg ttggtaataa acgcatcata
    56581 ctcacgctgc acaccagaaa cgtagacgcc attgccaacc cagccgccag cgcccaacgt
    56641 gctattggcc tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga
    56701 gatattatta cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt
    56761 attgatcaag ctggacatgg aggttcctta taagccttca attacagagt attatcggca
    56821 gcgacccgcc ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg
    56881 ctcaccttgt cgcttatcga tttcatctgc tggatcatgt tggtgagttt acgggcatag
    56941 tgaggatcgg tagcataccc cgcgtcctgt agggcctgcg ccccctgctc cgcactcgcg
    57001 gctgtcgtca cggcggcgta gcgcgggtta cgtgttaaca gcccaacgta atccgacaat
    57061 gcttccagat acgagctgta gacccgaaat ttcgctttta ctttcttcgc ttcgccattt
    57121 tcatattcag tcgtggtgat ttcagtaact ggacctttcc agttgccaga ggctttgacg
    57181 ccaaacaggt tatagctcgg ctcgccgttt tcgcggcgga tttgccgttg cccccaacca
    57241 gactccagcg ccgcctgagc gaggatcaaa tgatgtggca caccgctttg ctggcttgcc
    57301 agttgggcgg gcagcgagag ttgcgcgagg aatgctttac tgtcacccgg cagcgaatca
    57361 tcgtagttac gtggcacggc cttttgcacc agctgcgaaa gcgcctgatt ttgataacgc
    57421 accacagttt cgagcgggaa tttcatcggt gctgctggcg tggactcctc tggcaatggt
    57481 tgttctggcg tcatctgttt aaccatcatc tctgcaagcc ccagaccttt gcccgccgtc
    57541 atctgttggg caatctgctg gtcatacata ctggtataca ggcgagtgtg ctcgctgctg
    57601 aacaggccat cttttggtaa agcgtcgcgc atgcttttca acatcatctg cacgaacatc
    57661 ccttccacct gacgggccac cggacggata tttgccgccg gatcttcgcc cgctttcgcc
    57721 tttagttcgt tgagtgattg cgcatcccag gccgcacttg ccagtagttt gctgtcgctg
    57781 atcatcagat gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcagta
    57841 tggacatcag atccatcggc gtagcgccca gcgcattgag cgcgcgcacc acgttattga
    57901 ggctggcgct ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg
    57961 tttgtggagt aaccacagtc tgtccaccac caaacggtgt atctggctgg ctgacattgg
    58021 cctgacgatt gactgttact gagagatttc cctgcgctac cgcgcagctg tcgagggtca
    58081 cttcgcgatt catcaccacc gaaccggtgc gcgagttaat cactacttta gcgtcctgcg
    58141 gggtgacatt aacctgcata ttctggatat ctgcaaggaa acggacctgg gaactgttgc
    58201 cacttggtac gcgcacctga atagtccgcg catctaacgc ggtggcgctg ccatatccac
    58261 gcacgcggtt gatggtgtca gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt
    58321 gcaaattaag ggtattcccg acgccaaact ggctgggcaa ttcacgttca ataaccgcac
    58381 cattggtgat ccgtccaccg ttcagttggt tcacctgaac actgctaccg ccagcggagg
    58441 ctcctgcgcc gccaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc
    58501 ccttaagcgg cgtcatcaac aacgtacctc cacgcaagct tttggcattt cccatggaag
    58561 aaaccaccac atcgatggtt tgcccctgac gtccaaacgg aggaagtgac tctgtcacca
    58621 ttaccgcagc gacgtttttt agctgcatat tggtgcccgt cggaacggta attcccagct
    58681 gtgagagcat gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccgg
    58741 tgccatccag ccccaccacc agaccatagc caatcagtga gttttgcctt accccctgaa
    58801 cactggtgag atcgcgaata cgctcagcct gagccgccgt cgtaaccagt agaagaatta
    58861 atgcagagag aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa
    58921 acgctgcaac cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat
    58981 gcgcgcatcc gccacctgag tagacggtac ggtattgctg ccgctgatag tgcgtggatt
    59041 aaccacgcca gagaagcgaa taaattcggt accctgatta atggcaatct gtttttcacc
    59101 caccacatgc aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtgccgct
    59161 aaaggtattg ctggcattgg ccccgccctt tccgttgaac gtgttaccac cggaggcttc
    59221 gacatcggca cgagcgttac caaacagccc ctgcaaatag cgcggcacag tatcaaagcc
    59281 aaaattagtt ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctgacgtt
    59341 ctcctgcaac acgatggtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag
    59401 cggttgatag ccatagttaa tcggctgagc agactggaaa atagaaccgt tggcgacggg
    59461 cgtcggaccg ggaaccggtt gtgcactggt cgccccctgc accagcggcg tggagggtat
    59521 ccaggcgcag ccggttagtg aaagcaccaa caagctggaa atggcataag tatgcgcagc
    59581 gtttttttgc atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta
    59641 agccttagag ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc gctttactgt
    59701 tgatttcgta agcgcgttgc acctgaatca tattgaccag ttcttccgcc acgttgacgt
    59761 tagacgtttc aacataccct tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt
    59821 cgttcggtac accagaggat tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagcc
    59881 cggtgtcatt cataaaggtg gtgagattga gctgcccaac ctgaaccgga gctgcctggc
    59941 cttgttgggt tacgctgacc acgccatcac gaccgatggt gatacttaac gcattcgccg
    60001 gaatggtgat cgctggctgc acctgaaaac caccagctgc ccgttctgat ccacctggaa
    60061 agagccgtca cgggtatagg ctgatgaacc atctggcaac atcacctgga aaaagccctg
    60121 ccctttaatc gcgacatctt tgctgttgtt ggtctgcgac aggtttccct ggctgtgtaa
    60181 gcgttcagtg gcgaccgggc gtacccccgt gccgatttgt aatccggagg gtaaggtggt
    60241 ttgttcggaa gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac
    60301 cgcgcgctga cgcttaaaac cgttagtact gacgtttgcc aggttgttgg caatgacgtc
    60361 catattggtt tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa
    60421 atgtcctgta gtggattagt gacaaagtgc atatgtcttc ttgccggatg cggcgtaaac
    60481 gccttatccg gcctacaagt gctcgcaaaa tcgataaatt gcgattgccc ccatagaccc
    60541 gataaacttg cgcatcgggc aacttatact tgtcatgtat cctttcaatt aactcatcga
    60601 cagcagttgg ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat
    60661 ttcaaaacgc cgcgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact
    60721 gccttccaga acccccgaca tcacacgcaa ggttggatct gcctgcagta ccggccctcg
    60781 cgtggcctgg gtttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgct gcacttcgct
    60841 gcccgtggct ttcaccagtt tcagacgccc tactggcgca actgtatttg ccggatcgcc
    60901 cggattgagc gccgagattg tgccatcggc agcaatagtg atttccgctc cttccggcac
    60961 agcaattggc cccgcctcgc ctatcacctg atgcccctga attgtcagtt gcccggtggg
    61021 atcaacctga atgctgccat tacgcgtata cccttcgctg ccgtcagcgg tctgcacggc
    61081 cagccagcca tcctgctgca acgcgacgtc cagcgggcgc gaggtgtaat ccattttgcc
    61141 gggcgtcata tctgcgcccg gcgttgacgc cgtgaccaac gtgcgcgtgg gcagagaaag
    61201 cccttccact ggcaccgcgc gtaaagcatt caactgcgcg cgaaaaccgg gcgttgaggc
    61261 attggccaga ttactggcgg ttaccgcctg ttgattcagt gtctggctgg ctgctcccat
    61321 cgcggtataa attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca
    61381 gcgtgttgag gatctggtcc tgagttttga tggtctgggc gttagactga tagttacgct
    61441 gggcaacgat catattgacc agttctttac tgagatcgac attggacgct tccaacgcac
    61501 cgttggtcag ggtgccaaag ttgcccgtcc cggctgtccc caacagcgcc acgccagaag
    61561 attgcgtcgc agaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt
    61621 tcgccagtac aatctgcccc agcagttgtg tttgttcgtt ggaatagttg ccgacaaccg
    61681 taccgtcatc attgatttga taactcacca gatcgcccgg tttgtagcca ttctgggtgg
    61741 ttgccacaat attgttagcg ccggtatttt gctgcatgga gttgaggaag ctcaggctaa
    61801 acgtggcggg atctgcgccg ttaattgcgc cagtggtaat attctctgtt ggatttgagg
    61861 tcagaacgcc attggcatta aacaccagct tcattgcagg ctcggctgta cctgttggat
    61921 cactgctatc ctgggtgtag acctgccagt tattatcccc ggtcttcaca aagtagacgc
    61981 tcatgtcatg agcattaccc tgactgtcga aaacagtcac cgaacctttt ttgttatagc
    62041 tatccgcatt gctggcgctg aatggcgtaa cagtaggaag cggatcactg gaattcaggt
    62101 tgatctgcat cgacgccgtg gtggtagttt tcgctgccat cagggtattc gggatcgaaa
    62161 tattggtcgg attcgcccct tgctgaatag tcggcggcgt accggttgcc gggtaacccg
    62221 tcagctgtaa accttgcata ttcaccaggt tacggttttc atccagctta aattgtccgt
    62281 tacggctgta gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg ttctggctga
    62341 tagcaacgtc cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga
    62401 taccggcaac ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg
    62461 ccgtgcctga tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga
    62521 ggttggtggc ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct
    62581 gaaatgtgaa ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg ccgtaagtgc
    62641 cgagatccag cgtattaccg ctgttgccgc ggatcacacc ctgcaccaga gcaaactgca
    62701 gcggctgggc aaccagttgt gtaccaccgt tactggcgct aatcgctaca ttgtaagaac
    62761 cgttcggcgc agtgctgcca tcagtcaacg taccgtccca ggtgaaactg tggactccgg
    62821 cggtcagttc accaatatca atggtgcgca caaccgcgcc atttttatcg gtgatggtgg
    62881 ccgtaacttt gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc gtggtcacag
    62941 ccccttcttc actgccggtt cccgcaagaa cagtggtgcc ggggatcatc acgccgtgac
    63001 cgatcaggtt actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccga
    63061 gcgtggtatt gagtttttca atcccgctga ccgtgctgat ttgtgccaat tgcgacgtca
    63121 gctcgttgtt ttccattgga ttggtcgggt cctggttttt cagctgcgcc accaacaaag
    63181 tcagaaaact gctttgtaag tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg
    63241 tactgacgcc ggtatttgtc ggatcggtgg tggttaccgc aatggacata gctttctcct
    63301 ttattgaccg agcgtaaggg ttttcagcat catgcttttc accatgttga gcacttcaac
    63361 attggcctga tagctgcgtg acgccgacat ggtgttaacc atctccccga cgacatcaac
    63421 gttcggcatt tttacgtagc cctttgcatc cgccagcgga ttacccggtt cataaaccag
    63481 tttgtccggg gcctgacttt ctataacatc ggcaaccttt acgccgcctg tcgcagcacc
    63541 tggtgcagcg ttaacctgga ataccacctg ttttgcccga tatggctgtc catcgggacc
    63601 ggtcacgcta tcagcattcg ccagattact ggccgccacg ttcaagcgct gggactgggc
    63661 agttaacgcc gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct
    63721 ctgtaaaacg ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta
    63781 ttgcaggctg ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc
    63841 aagcgaaggc tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg
    63901 cgccggaatg tgttgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc
    63961 acgttgcatg acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg
    64021 ggtatcggca ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag
    64081 cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt
    64141 atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc
    64201 aaaatgggtc gctatttatg ccgttgatga ccattgcgga caggtacaat tcacgttgta
    64261 gaaatggctg ggggcgaaaa tgctggcaat aaaacgtagc gtggcgatca tctcgatact
    64321 attcactccg ttaagtgcgg cgagtaatct cacatcgcaa ttgcaaacct tttttagcgc
    64381 ccagctcgcg ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact
    64441 accgccgtgc gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt
    64501 gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg tacaggccac
    64561 aggaaattat gtggttgccg cgatgcccat tgtgcgggga ggaaagctgg aagctggcaa
    64621 tgtcaaacta aaacgcggac ggcaggatac cctgccaccg cgtacggtgc tggatatcaa
    64681 tcaacttgtt gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac
    64741 ccagtttcgc caggcatggc gggtaaaagc gggacaacgc gtcaacgtga tcgccagcgg
    64801 taacgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcacacagaa
    64861 tgcgcgggtg cgcatggtat cgggactggt ggtcagcggc gttgttgatg cagatgggaa
    64921 tattcttata aaccagtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac
    64981 acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct
    65041 gaagcccgta agcaccgttc aaccacgcga aaccactgac gcgtcggtaa cgaacacccg
    65101 ggcggcaaaa acaacagcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa
    65161 actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc
    65221 gattcgtaac ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga
    65281 agcgcagcaa gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc
    65341 agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcaagaacag caacatctct
    65401 ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc
    65461 tggcgacgct ggattacctc gaacagttac gcaggaaaga acccaataca gcaaatagcg
    65521 ttgatattag tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga
    65581 atcaacataa cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa
    65641 tgttgaaacc gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacaaccc
    65701 atcgcggcgg taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga
    65761 atgcattgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt
    65821 gcagcgaagt acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccaa
    65881 ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac
    65941 atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggagta
    66001 aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga
    66061 ccaatagaaa gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat
    66121 tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa
    66181 taaaagccag gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca
    66241 attaacgccc gctgggtcat cagcgcatca aacgcggtaa atttaccgta ctggaacagc
    66301 gaaacggtta acggacccga aagaatgccc aacgcaaccg cactcggcag cgccaacagg
    66361 aaacaaagac gcaaccccca gtccatcaaa cggttgtatt catcatgatt gccactggca
    66421 aaacttttcg acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccggag
    66481 ggaaactcca ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac
    66541 gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc
    66601 ggtcccatct gtttcaccac gcgcattgct ccggcatcgt ggaagttaat gcgcggcagg
    66661 accagcatgc cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg
    66721 accgtaacag cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc
    66781 gcgaaaccaa tcatgctgat gttaagcagt gttggcgcaa acgccggaat cgagaagcgg
    66841 ttccacgtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga
    66901 aaggtaatct ttagtagctg gctggtcagg gcaaatttgt cagctgtgtc agcgaagcct
    66961 ggcgcggtca ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc
    67021 agcgcaagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc
    67081 tgcttacttt tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca
    67141 aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc
    67201 cctgcgccaa agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac
    67261 atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccag
    67321 acccacgggc aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctggatt
    67381 atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat
    67441 cttgtcaacg atacgttgcg ccagcactgc ctgttcgccg gcggtttgca gaactgtctg
    67501 attttgcaca cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcaagagt
    67561 gctctgccaa ccaggaatcg gtttatgcac tacgccctgc ccgcgctcct cacgccattc
    67621 gcgcatatcc gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg
    67681 acttccggca cggcgatgca tacaggtggt gatctgcaaa ggatcggccg aaaaatggtg
    67741 ctcggcaaac agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc
    67801 tttgccgccc gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt
    67861 gaaataaaga tcgtgtggcc cgacactatt gctacgatgt ttatccattc ttagcgaagc
    67921 tgcggtagcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc
    67981 gaccatcaac gtcagttttt tccgcgccgc cagttccacc agccgttcag catcgcgcag
    68041 attttctgcc agcggtttat cgacacagac atgtacaccc gcattgagta acgtactgac
    68101 cacgtcaaag tggctggcgg tgctggaatg cacaaaaacc gcatcgcaac tggcggcaag
    68161 gctggataac gaatcggcat aaggaatgcg ccagctttca caaattggca gggctttcgc
    68221 gcgcgtaggc gaccaggctc cttgtaacat ccagtcagac gctgccgcca gcaccggtaa
    68281 ccacgctttt tgcgcaatgc cgcctaatcc cacaacgccg atacgtaatt ttttcacttt
    68341 aatctcccag atgggccagc aacgaatcaa gacgctgttt cagttctgcc acttcgattt
    68401 ccagggcttc gacgcgggcc tgtaaatcac catcaaccgc gttcgacata tccgttaccg
    68461 ccggctgatc ctctacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg
    68521 gttcgcgggc cagacgcacc acaaaaggac catcttcgcg atttgccagt tgttccagcg
    68581 tcgactccac ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt
    68641 cacccggcgt ctgggcacca cgcaataaca acgtggtgat taacgccact tccgctgcgc
    68701 tcagtttcag atcgccaaat tctgaattac aaaaacgttg ttcatatttg gtgacccgat
    68761 taccaaagcc actcaccgtg cgcagataat ggcgtttgac cagattatcc agttgctcct
    68821 gcacttcaga ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg
    68881 tgactacacc attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca
    68941 agcaaccaat cacgcgagct tcaagggcag taagttgata tttcatgaga ttctccttag
    69001 cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca
    69061 tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa
    69121 tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg
    69181 cgctgcatat aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat
    69241 ttttgcccaa tcgaataacc gagatagcag gcatgaaaag agccacgaac aacattggaa
    69301 aaattggcaa cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct
    69361 gaaccttgtt tatgaaattc gttaatcatc cccagcctgg cctgccagcc tgatggataa
    69421 cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa
    69481 taatccgcaa gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg
    69541 gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc
    69601 gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag
    69661 tccaaaatgc cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac
    69721 gttttgataa aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa
    69781 tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg
    69841 aacctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt
    69901 gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc
    69961 ggtattgctc tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca
    70021 attgccgacc gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc
    70081 ggattcgcca caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc
    70141 tcgggactcg gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc
    70201 cgtccacaac aacgttgtcg ttttttctcg ctgttgatga tgcaggacag tgccggtgcg
    70261 gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc
    70321 acaggggccg ttttgtttgt gctgtgtgcg gcgttcaatg cgtggttgtt accggcatgg
    70381 aaactctcca ccgtacgcac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag
    70441 cgttttgtca cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg
    70501 ctgatgctgc caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg
    70561 tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa
    70621 aagcattttc gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg
    70681 atgccggtgg gcatggtcag cggtctgcaa caacttttca ccctgatttg tctgttttat
    70741 atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgctttgct ggcggacgca
    70801 agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt
    70861 ggttatatcg gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt
    70921 ccgtggatga tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt
    70981 agtcagaaac gcgcaacgcg tcgtttgctg gaacgcgacg cctgatttga tactcccccc
    71041 tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac
    71101 atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc
    71161 cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt
    71221 caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggat
    71281 atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct
    71341 gccattgaag agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt
    71401 tttgccaaat cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac
    71461 aagaaagagg ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt
    71521 aagaatatca gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg
    71581 aatggcgaac tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg
    71641 gtagccggaa tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag
    71701 acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt
    71761 attcgcgggt tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca
    71821 tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt
    71881 tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca
    71941 atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa
    72001 agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgatcagcca
    72061 aattggtcgc gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa
    72121 ctccctgttc ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt
    72181 tgataaagaa aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca
    72241 accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg
    72301 caattacttt aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc
    72361 aatggcgaaa aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt
    72421 tacggattaa tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt
    72481 ccgcttatcc tttgtgtccg gcaaaaacat tccttcagcc ggagcataga gattaatgac
    72541 tgcaccatcc caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga
    72601 tggcgacatg ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt
    72661 aatgcccaat ctggctccgc ccgtgaccac agttgaggct gccgtggcgt atcgccaacg
    72721 tattcttgac gccgtacctg ccgggcacga tttcacccca ttgataacct gttatttaac
    72781 agattcgctg gatcctaatg agctggagcg cggatttaat gaaggcgtgt tcaccgctgc
    72841 aaaactttac ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc
    72901 aatcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga
    72961 agtgacacat gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat
    73021 ggaacctctg cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa
    73081 agatgctgcc gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca
    73141 gcatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg
    73201 tttacccatc ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg
    73261 ttttaatcga gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag
    73321 cagttgcggc tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt
    73381 ctttgaagag atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca
    73441 gttctatggg ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt
    73501 tgctgaaagc atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt
    73561 acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tatttaacct
    73621 gtataaataa ccagtatatt caacaggggg ctattatgcg aattgaagtc accatagcga
    73681 aaacttctcc attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta
    73741 ttcagtatgc gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcgaata
    73801 atttatcggt tattggcgca acaaaagaag ataaacagcg cattagcgaa attctccagg
    73861 aaacgtggga aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt
    73921 ttgccgggtc gccccggctt ttttatttta cttacgaatt ttgagtacaa a
//
LOCUS       DAZPEP010000004        72781 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZPEP010000004 DAZPEP010000000
VERSION     DAZPEP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72781
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.004"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHUT9_000220"
     CDS             complement(324..536)
                     /gene="rpmE"
                     /locus_tag="ACHUT9_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFR1346799.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            739..2937
                     /gene="priA"
                     /locus_tag="ACHUT9_000221"
     CDS             739..2937
                     /gene="priA"
                     /locus_tag="ACHUT9_000221"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFR1346800.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASEIPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3093..4118
                     /gene="cytR"
                     /locus_tag="ACHUT9_000222"
     CDS             3093..4118
                     /gene="cytR"
                     /locus_tag="ACHUT9_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFR1346801.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHUT9_000223"
     CDS             4210..5169
                     /gene="ftsN"
                     /locus_tag="ACHUT9_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFR1346802.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5262..5792
                     /gene="hslV"
                     /locus_tag="ACHUT9_000224"
     CDS             5262..5792
                     /gene="hslV"
                     /locus_tag="ACHUT9_000224"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFR1346803.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5802..7133
                     /gene="hslU"
                     /locus_tag="ACHUT9_000225"
     CDS             5802..7133
                     /gene="hslU"
                     /locus_tag="ACHUT9_000225"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFR1346804.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7200..8126
                     /gene="menA"
                     /locus_tag="ACHUT9_000226"
     CDS             7200..8126
                     /gene="menA"
                     /locus_tag="ACHUT9_000226"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFR1346805.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8219..8704
                     /gene="rraA"
                     /locus_tag="ACHUT9_000227"
     CDS             8219..8704
                     /gene="rraA"
                     /locus_tag="ACHUT9_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFR1346806.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHUT9_000228"
     CDS             complement(8789..9034)
                     /gene="zapB"
                     /locus_tag="ACHUT9_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFR1346807.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9460..10305
                     /gene="glpF"
                     /locus_tag="ACHUT9_000229"
     CDS             9460..10305
                     /gene="glpF"
                     /locus_tag="ACHUT9_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFR1346808.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10328..11839
                     /gene="glpK"
                     /locus_tag="ACHUT9_000230"
     CDS             10328..11839
                     /gene="glpK"
                     /locus_tag="ACHUT9_000230"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFR1346809.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            11969..12979
                     /gene="glpX"
                     /locus_tag="ACHUT9_000231"
     CDS             11969..12979
                     /gene="glpX"
                     /locus_tag="ACHUT9_000231"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFR1346810.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13076..13822
                     /gene="fpr"
                     /locus_tag="ACHUT9_000232"
     CDS             13076..13822
                     /gene="fpr"
                     /locus_tag="ACHUT9_000232"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFR1346811.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHUT9_000233"
     CDS             complement(13827..14255)
                     /gene="uspD"
                     /locus_tag="ACHUT9_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFR1346812.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14282..14581)
                     /locus_tag="ACHUT9_000234"
     CDS             complement(14282..14581)
                     /locus_tag="ACHUT9_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFR1346813.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14793..15233)
                     /locus_tag="ACHUT9_000235"
     CDS             complement(14793..15233)
                     /locus_tag="ACHUT9_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1346814.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15334..15933
                     /locus_tag="ACHUT9_000236"
     CDS             15334..15933
                     /locus_tag="ACHUT9_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFR1346815.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHUT9_000237"
     CDS             16041..16808
                     /gene="tpiA"
                     /locus_tag="ACHUT9_000237"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFR1346816.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHUT9_000238"
     CDS             complement(16863..17618)
                     /gene="cdh"
                     /locus_tag="ACHUT9_000238"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFR1346817.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHUT9_000239"
     CDS             complement(17725..18714)
                     /gene="sbp"
                     /locus_tag="ACHUT9_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFR1346818.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHUT9_000240"
     CDS             complement(19034..19996)
                     /gene="pfkA"
                     /locus_tag="ACHUT9_000240"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFR1346819.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHUT9_000241"
     CDS             complement(20177..21079)
                     /gene="fieF"
                     /locus_tag="ACHUT9_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFR1346820.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHUT9_000242"
     CDS             complement(21314..21532)
                     /gene="ogrK"
                     /locus_tag="ACHUT9_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFR1346821.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(21614..22777)
                     /locus_tag="ACHUT9_000243"
     CDS             complement(21614..22777)
                     /locus_tag="ACHUT9_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR1346822.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDITLGAIVEAIASRNRLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHCLNNSGFTTSLELEVRLSDVEYETEDDE"
     gene            complement(22777..23256)
                     /locus_tag="ACHUT9_000244"
     CDS             complement(22777..23256)
                     /locus_tag="ACHUT9_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1346823.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENITLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISKVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAG"
     gene            complement(23271..25718)
                     /locus_tag="ACHUT9_000245"
     CDS             complement(23271..25718)
                     /locus_tag="ACHUT9_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1346824.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRRASKLLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAAVGINTRNLAHDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTIAGVKLLMPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWVQKNKSLASTIGLIAGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPVVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHISLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(25711..25866)
                     /locus_tag="ACHUT9_000246"
     CDS             complement(25711..25866)
                     /locus_tag="ACHUT9_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR1346825.1"
                     /translation="MTLPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(25863..26138)
                     /locus_tag="ACHUT9_000247"
     CDS             complement(25863..26138)
                     /locus_tag="ACHUT9_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1346826.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(26195..26713)
                     /locus_tag="ACHUT9_000248"
     CDS             complement(26195..26713)
                     /locus_tag="ACHUT9_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR1346827.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(26726..27916)
                     /locus_tag="ACHUT9_000249"
     CDS             complement(26726..27916)
                     /locus_tag="ACHUT9_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFR1346828.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTVSEVKAYRQNFSQRELMVIWPDFLAWDTVASTTATAYATARALGLRAKI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITDKYLANLVTS
                     VNSN"
     gene            28327..29745
                     /locus_tag="ACHUT9_000250"
     CDS             28327..29745
                     /locus_tag="ACHUT9_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1346829.1"
                     /translation="MTYALIDNSTLTAVQRIEGKVKTRSKDSVDTDIIAFENYIQSIL
                     FYDHVIAVDDYIPEYRGSRISSFREILFLDKAQYGLDEIESEAKKISDALKPKIRGGT
                     FVNDDFKKLIELLQTHIICTWDISSSVYHLTLKNLSDGGKEFEKYGNIAAAIFSELRD
                     AAESGNRTNGDVELLDRFGNPIRKGYKVPGAKWGDGTSAGEASDAIKAFVASLVWLAN
                     RAVFYSLTAKYLQADTFLYPLRQAYQQTYLSQNCQYGFDYAKHIVENFSTSLSHDLID
                     IHSGGKAVATATSLPLFSAWLAKETGDPSCIVEAAYNIRDNNEFVRAREKMREIKRLF
                     DENELPQANKSVRALLTEVSKESDYIRTKYGLKTRQGVPVTKLVHVYNTVAAINSLPK
                     LPTFNFKVKVPDFLYDLKKPKGFRALYRNLTNDLSTVWSLGEARDILGSKVVIDNHAR
                     IYSPKHEEPRFKNSHSPYKSPM"
     gene            complement(29860..30387)
                     /locus_tag="ACHUT9_000251"
     CDS             complement(29860..30387)
                     /locus_tag="ACHUT9_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1346830.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKDFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIRYVEKDVTAIRPDGLSVVEVPDITANRRADISGNWMFKDCTVI
                     KRIYTAEELQQQAENRKARLLADAESVILPLERAVRLNMATDEERSRLEAWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(30391..32709)
                     /locus_tag="ACHUT9_000252"
     CDS             complement(30391..32709)
                     /locus_tag="ACHUT9_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1346831.1"
                     /translation="MSTKFRTVITTAGAAKLAAATAPGGRKVNITTMAVGDGGGKLPV
                     PDAGQTGLIHEVWRHTLNKISQDKRNSNYIIAELVIPPEVGGFWMRELGLYDDAGTLI
                     AVANMAESYKPALAEGSGRSQTCRMVIIVSSVASVELTIDTTTVMATQDYVDDKIAEH
                     EQSRRHPDASLTAKGFTQLSSATNSTSETLAATPKAVKAAYDLANGKYTAQDATTARK
                     GLVQLSSATNSTSETLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSATNSDSE
                     TLAATPKAVKTAYDLANGKYTAQDATTARKGLVQLSSAINSDSETLAATPKAVKSAYD
                     NAEKRLQKDQNGADIPGKDTFTKNIGACRAYSGALSTEAGNWTTAQFIEWLDSRGAFN
                     HPYWMCKGSWSYANNKIITDTGCGDIHLAGCVVEVMGTKSAITIRVTTPTTSSGGGTT
                     SAQFTYINHGDGYSPGWRRDWNRQGDSMTGTINQDGGSQNAYMSTALCSGTRGGKKYL
                     RKFRGGEGDTIWHETVQGGVIRWATGNNDAQEELSLSSAYGLRSRGEITSLSANGLRI
                     AYGNYGFFIRNDGGSTYLMLTASGDKFGTWNGLRPLTINNANGGVSMGHGLSVTGDIV
                     SSTKVRAGSGKKFTVSSSNTSTKEAAFNLWGNSSRPVVAELGDDAGWHFYSQRNTDNS
                     ITFAVNGQVSPSNYGNFDSRYVRDIRLGGAATYKPANNGMTWTHQAPSGCVYSGIIVQ
                     DTGSNSADNIGGVYYRPVQKYINGTWYNVAQV"
     gene            complement(32720..33250)
                     /locus_tag="ACHUT9_000253"
     CDS             complement(32720..33250)
                     /locus_tag="ACHUT9_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR1346832.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVRIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWENSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(33243..34151)
                     /locus_tag="ACHUT9_000254"
     CDS             complement(33243..34151)
                     /locus_tag="ACHUT9_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFR1346833.1"
                     /translation="MPIIDLNQLPAPDVVEELDFETILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLI
                     ITPADDSTLPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELTAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(34156..34503)
                     /locus_tag="ACHUT9_000255"
     CDS             complement(34156..34503)
                     /locus_tag="ACHUT9_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR1346834.1"
                     /translation="MTARYLGMNRSDGLTVSDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEARVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(34500..35135)
                     /locus_tag="ACHUT9_000256"
     CDS             complement(34500..35135)
                     /locus_tag="ACHUT9_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1346835.1"
                     /translation="MNTLANIQELARALRNMIRTGIIVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSVGEQVLILAVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NKLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGSPL"
     gene            complement(35202..35654)
                     /locus_tag="ACHUT9_000257"
     CDS             complement(35202..35654)
                     /locus_tag="ACHUT9_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1346836.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYAPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEETRKDGKKIHYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(35647..36114)
                     /locus_tag="ACHUT9_000258"
     CDS             complement(35647..36114)
                     /locus_tag="ACHUT9_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1346837.1"
                     /translation="MLKPDSLRRALTDAVTVLKTSPEMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPPPPEPVTRPMELYINGELVSKWDE"
     gene            complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHUT9_000259"
     CDS             complement(36077..36322)
                     /gene="lysC"
                     /locus_tag="ACHUT9_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979584.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFR1346838.1"
                     /translation="MLCAGCTSAQPAQTPVIVHNACPKVSLCPMPGSDPQTNGDLSAD
                     IRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHUT9_000260"
     CDS             complement(36222..36647)
                     /gene="lysB"
                     /locus_tag="ACHUT9_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585210.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFR1346839.1"
                     /translation="MSKLMIVLVVLLSLAVAGLFLVKHKNASLRALLDRANNVASEQQ
                     TTITMLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKTITRLLNENEDFRRW
                     YGADLPDAVRRLHQRTACADASDCPQRLPESEPLPDAGQ"
     gene            complement(36635..37060)
                     /locus_tag="ACHUT9_000261"
     CDS             complement(36635..37060)
                     /locus_tag="ACHUT9_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFR1346840.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     VIAGGVGVYAAEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALMVMVATFIS
                     LVAAGWIFTALIYLVASLFFRLIRTACRQRLEGREPCQN"
     gene            complement(37075..37572)
                     /locus_tag="ACHUT9_000262"
     CDS             complement(37075..37572)
                     /locus_tag="ACHUT9_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFR1346841.1"
                     /translation="MPVISTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(37572..37853)
                     /locus_tag="ACHUT9_000263"
     CDS             complement(37572..37853)
                     /locus_tag="ACHUT9_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1346842.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKP"
     gene            complement(37857..38060)
                     /locus_tag="ACHUT9_000264"
     CDS             complement(37857..38060)
                     /locus_tag="ACHUT9_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR1346843.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVEAVLAANPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(38060..38569)
                     /locus_tag="ACHUT9_000265"
     CDS             complement(38060..38569)
                     /locus_tag="ACHUT9_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1346844.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFSRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(38669..39412)
                     /locus_tag="ACHUT9_000266"
     CDS             complement(38669..39412)
                     /locus_tag="ACHUT9_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFR1346845.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDPRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALVHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRTPRKTRSVTPAKRGRPKKKAS"
     gene            complement(39416..40488)
                     /locus_tag="ACHUT9_000267"
                     /pseudo
     CDS             complement(39416..40488)
                     /locus_tag="ACHUT9_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
     gene            complement(40547..41401)
                     /locus_tag="ACHUT9_000268"
     CDS             complement(40547..41401)
                     /locus_tag="ACHUT9_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1346846.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQEVTDRADETSQAFTRLKNSLDHTESLTQQRRSKATGGG
                     GDALMTNC"
     gene            41575..43347
                     /locus_tag="ACHUT9_000269"
     CDS             41575..43347
                     /locus_tag="ACHUT9_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFR1346847.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNIANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKVSVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            43347..44381
                     /locus_tag="ACHUT9_000270"
     CDS             43347..44381
                     /locus_tag="ACHUT9_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1346848.1"
                     /translation="MSKKKGKTPRPAAKTITASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFAPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            44753..45895
                     /locus_tag="ACHUT9_000271"
     CDS             44753..45895
                     /locus_tag="ACHUT9_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HFR1346849.1"
                     /translation="MDIIDLKSQLENEDNAKKRAELLAQLEVIKQNAEEQIRAEQKDV
                     DFETKEFTVELLVNKYHNGLEDDTNELFVPDYQRDFVWSEKRQSKLIESLILGFPIPY
                     IFIADVLSEDPDLDGRIEIVDGSQRVRTIHAFIQNELVLQDLKSLDVLNGFKFQDLPL
                     SRQRRFMRIPVRVIELSSKCNEETRRDLFERINSGSDILKDMEVRKGSELGSTNLYTQ
                     VIKPCSTIPKFKELAPLSQTKEKRDERLEFTLRFFAYVDKYKNFDHSVRDFLNDYMSE
                     NRDIDETKQNSMKQEFANVLSFVENYFPAGFKKTITAKSTPRVRFESLAVGAALALRE
                     VPDLVPKNLDWLSSEEFKRLTTSDGANSRVKVIERIEYVRDKLLED"
     gene            45897..46577
                     /locus_tag="ACHUT9_000272"
     CDS             45897..46577
                     /locus_tag="ACHUT9_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAE_28990/MAE_18760 family HEPN-like nuclease"
                     /protein_id="HFR1346850.1"
                     /translation="MDRFREDFDERSGEILAYLDLLKFIEYAGAELISADDKEHKFSI
                     TAQSRKTLKGAVYILLYNLIESTMREAICLIHETIYDRNVEFDKLRKNLRSEILKRLK
                     NESVNVESLVNHLTKGISCGISYGTFNKKKIFSGNIDREEIKEKSKIYGFSTHSDYTH
                     TKHGEKLATVKQHRNDLSHGNVSFAEIGKNVSYQDLENISLEVIAYLDAIVNNIEHYI
                     NNNEYLEQ"
     gene            complement(46607..47629)
                     /locus_tag="ACHUT9_000273"
     CDS             complement(46607..47629)
                     /locus_tag="ACHUT9_000273"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFR1346851.1"
                     /translation="MTVKVIDLFCGAGGLTHGLQLAGLDVVAGIDLEGECRFPYERNN
                     KSKFIEQDIAKVTKEELLRLYGDASVKVLAGCAPCQPFSKYTQGKDKADDKKWPLLYE
                     FERLIREVSPEIVTMENVPDVTKHKVYNDFYNSLLKLGYYVWASKIDCVEYGIPQNRS
                     RHVLLASKLGAIELVKRDDVILKTVRDAIGNLPPLEDGQTDPNDILHRASKLNPINKK
                     RIIHSVPGGTWKDWPKELVAACHMKSSGKGYASVYGRMSWDKPSPTITTLCYGFGNGR
                     FGHPEQHRAISLREAALLQTFPMDYIFVEDKNKFVIRSIGKMIGNAVPVELGKAIGQS
                     IKNHLE"
     gene            complement(47716..50013)
                     /locus_tag="ACHUT9_000274"
     CDS             complement(47716..50013)
                     /locus_tag="ACHUT9_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR1346852.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASMLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWISQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDSGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDE
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWNDPEVVRALRGALKHGLRRPNRQ
                     QRNGSPLKPHEIAPSARLTRSERMQITRIRVDLAQNGIRPQRWELEVLARGATVNYDG
                     KKFTYPLADEWSGFSTVLESNSVKR"
     gene            complement(50003..50278)
                     /locus_tag="ACHUT9_000275"
     CDS             complement(50003..50278)
                     /locus_tag="ACHUT9_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR1346853.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(50275..50499)
                     /locus_tag="ACHUT9_000276"
     CDS             complement(50275..50499)
                     /locus_tag="ACHUT9_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFR1346854.1"
                     /translation="MPDNIDFIQEQQAELLERQINAARVKHCGASALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(50502..50801)
                     /locus_tag="ACHUT9_000277"
     CDS             complement(50502..50801)
                     /locus_tag="ACHUT9_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFR1346855.1"
                     /translation="MSCSHSVVLLNNALKIAVMKNGDLSLIQLCLDKEKRDITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTELASYCADVSRKLNDKRS"
     gene            complement(50801..51025)
                     /locus_tag="ACHUT9_000278"
     CDS             complement(50801..51025)
                     /locus_tag="ACHUT9_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFR1346856.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(51089..51589)
                     /locus_tag="ACHUT9_000279"
     CDS             complement(51089..51589)
                     /locus_tag="ACHUT9_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFR1346857.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMRFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(51759..52031)
                     /locus_tag="ACHUT9_000280"
     CDS             complement(51759..52031)
                     /locus_tag="ACHUT9_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cox family DNA-binding protein"
                     /protein_id="HFR1346858.1"
                     /translation="MDAENYVIQYPLDAVHVDKFADLLGKPKTAVSEMVKANKLPIIE
                     LRDPCKPKARAGEKWVFIPEFNRAVREAFYNRPVEQRDAWLLWMGL"
     gene            52168..52461
                     /locus_tag="ACHUT9_000281"
     CDS             52168..52461
                     /locus_tag="ACHUT9_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1346859.1"
                     /translation="MKLSEKIKALREAEGLSQSKFCEIIELPLSTLKKYEGGNFEPGG
                     TALLKITMHPTFQKYALWLMTDKTAPDAGQIAPALAHIGPESTESNHSAKRIG"
     gene            52531..53511
                     /locus_tag="ACHUT9_000282"
     CDS             52531..53511
                     /locus_tag="ACHUT9_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1346860.1"
                     /translation="MAIKKLDDGRYEVDIRPRGRDGKRIRRKFERKAEALAFERYTIA
                     NASQKEWGGQRADRRTLSELLDIWWKYHGQNHEHGTKEFNHLLKTISGIGDIPVSRMS
                     KRALMDYRSMRLRDGISAATINRDMYRLSGMFTKLIQLDEFSGQHPIHGLPPLAEANP
                     EMTFLEKAEIEKLLNVLAGDDLLVALLCLSTGGRWTKVATLKPAQITNCRVTFLKTKN
                     GKKRTVPISEELEKKVKEEASAKLFKVDYEKFCGILRRVKPDIPPNQATHILRHTFAS
                     HFMMNGGNIIALQQILGHASIQQTMAYAHLAPDYLQNAVALNPLKGGVTL"
     gene            complement(53697..54197)
                     /gene="cpxP"
                     /locus_tag="ACHUT9_000283"
     CDS             complement(53697..54197)
                     /gene="cpxP"
                     /locus_tag="ACHUT9_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFR1346861.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            54347..55045
                     /gene="cpxR"
                     /locus_tag="ACHUT9_000284"
     CDS             54347..55045
                     /gene="cpxR"
                     /locus_tag="ACHUT9_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFR1346862.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            55042..56415
                     /gene="cpxA"
                     /locus_tag="ACHUT9_000285"
     CDS             55042..56415
                     /gene="cpxA"
                     /locus_tag="ACHUT9_000285"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFR1346863.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(56521..57195)
                     /gene="yiiM"
                     /locus_tag="ACHUT9_000286"
     CDS             complement(56521..57195)
                     /gene="yiiM"
                     /locus_tag="ACHUT9_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFR1346864.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(57344..58327)
                     /gene="kdgT"
                     /locus_tag="ACHUT9_000287"
     CDS             complement(57344..58327)
                     /gene="kdgT"
                     /locus_tag="ACHUT9_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFR1346865.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(58587..59207)
                     /gene="sodA"
                     /locus_tag="ACHUT9_000288"
     CDS             complement(58587..59207)
                     /gene="sodA"
                     /locus_tag="ACHUT9_000288"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFR1346866.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            59492..60526
                     /gene="rhaT"
                     /locus_tag="ACHUT9_000289"
     CDS             59492..60526
                     /gene="rhaT"
                     /locus_tag="ACHUT9_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFR1346867.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(60523..61371)
                     /gene="rhaR"
                     /locus_tag="ACHUT9_000290"
     CDS             complement(60523..61371)
                     /gene="rhaR"
                     /locus_tag="ACHUT9_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFR1346868.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(61445..62281)
                     /gene="rhaS"
                     /locus_tag="ACHUT9_000291"
     CDS             complement(61445..62281)
                     /gene="rhaS"
                     /locus_tag="ACHUT9_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFR1346869.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            62569..64038
                     /gene="rhaB"
                     /locus_tag="ACHUT9_000292"
     CDS             62569..64038
                     /gene="rhaB"
                     /locus_tag="ACHUT9_000292"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFR1346870.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            64035..65294
                     /gene="rhaA"
                     /locus_tag="ACHUT9_000293"
     CDS             64035..65294
                     /gene="rhaA"
                     /locus_tag="ACHUT9_000293"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFR1346871.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            65536..66360
                     /gene="rhaD"
                     /locus_tag="ACHUT9_000294"
     CDS             65536..66360
                     /gene="rhaD"
                     /locus_tag="ACHUT9_000294"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFR1346872.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            66370..66684
                     /gene="rhaM"
                     /locus_tag="ACHUT9_000295"
     CDS             66370..66684
                     /gene="rhaM"
                     /locus_tag="ACHUT9_000295"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFR1346873.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            66985..67431
                     /locus_tag="ACHUT9_000296"
     CDS             66985..67431
                     /locus_tag="ACHUT9_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFR1346874.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            67442..68893
                     /locus_tag="ACHUT9_000297"
     CDS             67442..68893
                     /locus_tag="ACHUT9_000297"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFR1346875.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLVLNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            68883..69953
                     /locus_tag="ACHUT9_000298"
     CDS             68883..69953
                     /locus_tag="ACHUT9_000298"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1346876.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            69953..71701
                     /locus_tag="ACHUT9_000299"
     CDS             69953..71701
                     /locus_tag="ACHUT9_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFR1346877.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(71751..>72781)
                     /locus_tag="ACHUT9_000300"
     CDS             complement(71751..>72781)
                     /locus_tag="ACHUT9_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFR1346878.1"
                     /translation="LLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPEAPV
                     AKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQGPT
                     GKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEM
                     DKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELKNIE
                     EREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMN
                     FSFINSADQYQLETKKYVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEM
                     VDDYNSLR"
BASE COUNT        17504 a        19176 c        18407 g        17694 t
ORIGIN      
        1 gcaccgagca gcggattgac cggatggccg gacgtcaccc cgccggggca caccgaacag
       61 gccacgccgg tgggggcacc gatgcgcacc ccggctgaac cctggacaat gctaccgcca
      121 tactgcgtca tgtcgccctg acgcgccgcc ggttttccgc tcatcgtaaa atccttttga
      181 aataaataag aaccagaagc ctagcgccag tcaacaacag gaaatgataa tgagttaaca
      241 ccccggaatt tttcagaatt tataaaaggc aaattacagg cacaaaaaaa gcgccgtgcg
      301 gcgctttttt cggaaatccg gtcttatttg ctgcccggga tgttgaaacg cttgttgaag
      361 cggtcaacac ggccaccggt agcaacatca cgctgtttgc cagtgaagaa cgggtggcac
      421 ttgctgcaca cgtcgaggtt caggtcatga ccaacggtgg agcggatttt cattacgtta
      481 ccgcaagagc agctagcagt aatttcttcg tatttcgggt gaatatcttt tttcatggga
      541 aaacctcggt ttaaggccgc gtcgctcttc cagccctaac gccagacacc acgcgatgtt
      601 aaaagtatag cttcaatacg atcatttcgt acgaaggcgc gaaatcatac agaaattaac
      661 cagcgtatgc aaactgatcc gcactcttct acggcaatgt gtatactaac ccaccgaatt
      721 tcaagtcagg atgatgctat gcccgttgcc cacgttgcct tgcccgttcc gcttcctcgt
      781 acctttgact atctgctgcc agaaggcatg gcggttaaag ccgggtgtcg cgtgcgcgtg
      841 ccgtttggca aacagcagga gcgcatcggg gttgtggtat cagttagcga tgtcagtgag
      901 ctgccgctta acgagctgaa agcggtagtt gaagtgctgg acgttgagcc ggtatttact
      961 cactccgtct ggcgattact gctctgggcg gcagattact atcatcatcc gattggcgat
     1021 gtgctgtttc atgccttgcc gattttactg cgccaggggc ggcctgcggc gaacgcgccg
     1081 atgtggtact ggtttgccac tgaacaaggc caggcggtgg atctgaacag cctgaaacgc
     1141 tcacccaagc aacaacaggc gctggcggcg ttacggcaag ggaaaatctg gcgcgaccag
     1201 gttgccacgc tcgaatttaa tgatgccgcg ttgcagacgc tgcgcaaaaa aggtctgtgt
     1261 gatttagcaa gtgaaatacc agagtttagc gactggcgaa cgaactatgc cgtttctggt
     1321 gagcggttgc gattgaatac cgaacaggcc accgccgttg gcgcaattca tagcgcggca
     1381 gatacttttt ctgcctggct gctggcgggc gttaccggtt ccggtaaaac ggaggtttat
     1441 ctcagtgtac tggaaaacgt gctcgctcag ggcaaacagg cgctggtgat ggtgccggaa
     1501 atcggcctga caccgcaaac tatcgcccgt tttcgtgaac ggtttaatgc gcccgtggaa
     1561 gttctgcatt ccggtctgaa cgacagcgag cgtctttcgg cgtggctgaa agcgaaaaat
     1621 ggcgaggcgg cgattgtgat cggcacccgc tccgcgctgt ttacgccgtt taaaaatctc
     1681 ggcgtgattg tcattgacga agagcacgac agttcctaca agcagcagga aggctggcgc
     1741 tatcatgccc gcgacctggc ggtgtatcgt gcgcatagcg agcaaatccc gattattctt
     1801 ggctccgcaa cgcccgcgct ggaaacgttg tgcaacgtcc agcagaaaaa ataccgcctg
     1861 ctgcgcctga cgcgccgggc ggggaatgcg cgtccggcaa ttcaacacgt gctggattta
     1921 aaaggtcaga aggtgcaggc aggtctggct ccggcgttaa tcacccgtat gcgccagcat
     1981 ttacaggcca acaatcaggt tatcctcttt cttaaccgcc gtggctttgc gcctgcgcta
     2041 ctgtgccacg actgcggctg gattgccgaa tgcccacgtt gcgatcacta ctacacgctg
     2101 catcaggcgc aacagcattt gcgctgtcac cactgtgaca gtcagcgtcc ggtgccgcgc
     2161 cagtgccctt cctgcggttc cacgcacctg gtacccgtgg ggctgggcac cgaacagctt
     2221 gaacagacgc ttgcgccgct gttccctgat gtgcccattt ctcgtatcga ccgcgatacc
     2281 accagccgca aaggggcgct ggaacagcaa ctggcggaag tacatcgcgg cggtgcgagg
     2341 attttgatcg gcacacaaat gctggcgaaa ggtcaccact ttccggatgt gacgctggtg
     2401 gcgttactgg atgtggacgg cgcgctgttt tctgccgatt ttcgctcggc agagcgtttc
     2461 gctcagcttt acacccaggt cgccggtcgt gccgggcgtg cgggtaaaca gggcgaagtg
     2521 gtgctgcaaa cgcaccatcc ggaacatcct ctgttgcaaa cgttgctcta taaaggctac
     2581 gacgcctttg ccgaacaggc gctggctgag cgacgcatga tgcagctgcc gccgtggacc
     2641 agccatgtga ttgtgcgtgc ggaagatcat aacaatcagc atgcgccgct gttcctgcaa
     2701 caactgcgta atctgatcct ctccagcccg ctggcagacg acaaattgtg ggttctcggt
     2761 ccggttccgg cactggcacc taaacgtggc ggtcgctggc gctggcagat attgttgcag
     2821 cacccttccc gcgtgcgctt gcaacacatc attaacggta cgctggcact catcaataca
     2881 ataccggatt cccgtaaggt gaaatgggtg ctggatgttg atccgattga gggttaaacc
     2941 gctcacgatg cgaggcggat cgaaaaattc aatattcatc acacttttca tgaaaattct
     3001 gtaaccgttt tcacgcgcta tctgctaaaa atgttgccga tgtgaagtaa acatggatgt
     3061 agtacgcctg acgtgccagg cgaggagtga gtgtgaaagc gaagaagcag gaaactgccg
     3121 cgaccatgaa agacgttgcc ctcaaggcaa aagtctctac agcgactgtc tcccgagcat
     3181 taatgaatcc cgataaagtc tcccaggcca cccgtaatcg ggttgaaaaa gcggcccggg
     3241 aagtgggtta tttaccgcag cctatggggc gcaacgtcaa gcgtaatgaa tcccgcacca
     3301 ttctggtgat tgtcccggat atctgcgatc ccttctttag cgaaattatt cgcggtatcg
     3361 aagttacggc ggcaaatcac ggatatctgg tgctgattgg cgactgtgcg catcaaaatc
     3421 agcaggaaaa aacctttatc gatttgatca tcaccaagca aattgatggc atgttgctgc
     3481 tgggttcaag gctgccgttt gatgccagca ttgaggaaca gcgtaatctg ccgccgatgg
     3541 tgatggcgaa cgaattcgca ccggaactgg agctacctac agttcatatc gacaatctga
     3601 ccgccgcatt tgatgcagta aattatctat atgagcaagg gcataaacgg attggctgta
     3661 tagccggtcc cgaagagatg ccgctgtgtc actaccgcct gcaaggttac gttcaggcgc
     3721 tgcgtcgctg cggcattatg gttgatccgc aatacatcgc ccgtggcgac ttcaccttcg
     3781 aagccggaag caaagcgatg cagcagctgc ttgatcttcc acaaccgcct actgctgtct
     3841 tctgccatag cgatgtgatg gcgctcggcg cactttctca ggcaaaacgc caggggctga
     3901 aagtcccgga agacctttcc ataatcggtt ttgataacat cgacctgacg caattttgtg
     3961 atccgccgct gacaaccatc gcgcagccgc gttacgaaat cggtcgggaa gctatgctgt
     4021 tattgcttga tcaaatgcag gggcaacacg ttggcagtgg ctctcgttta atggactgcg
     4081 aacttatcat ccggggatca acacgcgcgt taccttaaag taaaccttta agactgccgt
     4141 gactggtcaa aggcccgccg cttaagtaac atggcgggct gacgaacgaa taaatacagc
     4201 gaaacgatag tggcacaacg agattatgta cgccgcagcc aaccggcacc ttcgcggcga
     4261 aaaaagagca cctcacggaa aaagcaacga aatctgcctg cggtttctcc cgctatggtc
     4321 gctattgctg ccgccgtgct tgtgaccttt atcggtggtc tgtacttcat tacgcatcac
     4381 aagaaagaag agtccgagac gctgcaaagc cagaaagtga ccggtaacgg actaccacca
     4441 aaaccagaag aacgctggcg ctacattaaa gagctggaaa gtcgccagcc gggtgtgcgt
     4501 gcgcccacag aaccttctgc cggtggtgaa gtgaaaacgc cggagcaact gacaccagaa
     4561 caacgtcagc ttcttgaaca aatgcaggct gatatgcgcc agcagccaac gcagctggtt
     4621 gaagttccgt ggaatgaaca gacgccggaa cagcgtcagc aaacgctaca acgccagcgt
     4681 caggcgcagc aattagcgga gcagcaacgc ctggctcagc agtcgcgtac gactgaacaa
     4741 agctggcagc agcagacgcg tacgtcgcaa gccgcgccgg tacaggcgca accgcgccag
     4801 tctaaaccgg cttctactca gcagccgtac caggatctgc tgcaaacccc tgcgcacacg
     4861 accgcgcagt cgaagccgca gcaagctgcg ccggttgctc gtgccgctga cgcgccaaaa
     4921 ccgacggcgg agaaaaaaga cgaacgccgc tggatggtgc agtgcggttc gttcagaggc
     4981 gcggaacagg cagagacggt acgtgctcag ctggcgttcg aaggatttga ctcgaaaatc
     5041 accaccaaca atggctggaa tcgtgtggtc attggcccgg tgaaaggcaa agagaacgca
     5101 gacagcaccc tcaatcggtt gaagatggcg ggtcatacaa actgcattcg gctcgccgcc
     5161 gggggttgaa accctcaaaa tcccccccat ctataattgc attatgcccc gtacttttgt
     5221 acggggtttg tactctgtat tcgtaaccaa ggggtcagct cgtgacaact atagtaagcg
     5281 tacgccgtaa cggccatgtg gtcatcgctg gtgatggtca ggccacgttg ggcaataccg
     5341 taatgaaagg caacgtgaaa aaggtccgcc gtctgtacaa cgacaaagtc atcgcgggct
     5401 ttgcgggcgg tactgcggat gcttttacgc tgttcgaact gtttgaacgt aaactggaaa
     5461 tgcatcaggg ccatctggtc aaagccgccg ttgagctggc aaaagactgg cgtaccgatc
     5521 gcatgctgcg caaacttgaa gcactgctgg cagtcgcgga tgaaactgca tcgcttatca
     5581 tcaccggtaa cggtgacgtg gtgcagccag aaaacgatct tattgctatc ggctccggcg
     5641 gcccttacgc ccaggctgcg gcgcgcgcgc tgttagaaaa cactgaactt agcgcccgtg
     5701 aaattgctga aaaggcgttg gatattgcag gcgacatttg catctatacc aaccatttcc
     5761 acaccatcga agaattaagc tacaaagcgt aaggatctcc catgtctgaa atgaccccac
     5821 gcgaaatcgt cagcgaactg gataagcaca tcatcggcca ggacaacgcc aagcgttctg
     5881 tggcgattgc tctgcgtaac cgctggcgtc gcatgcagct caacgaagag ctgcgccatg
     5941 aagtgacccc gaaaaatatc ctgatgatcg gcccgaccgg tgtcggtaaa actgaaatcg
     6001 cccgtcgtct ggctaagctg gcgaatgcgc cgttcatcaa agttgaagcg accaaattca
     6061 ccgaagtggg ctatgtcggt aaggaagtgg attctattat tcgcgatctg accgatgccg
     6121 ccgtgaaaat ggtacgcgtc caggctatcg agaaaaaccg ttatcgcgct gaagaactgg
     6181 cagaagaacg tattctcgac gtgctgatcc cacctgctaa aaacaactgg ggacagaccg
     6241 aacagcagca ggaaccgtcc gctgctcgtc aggcattccg caaaaaactg cgtgaaggcc
     6301 agcttgatga caaagaaatc gagatcgatc ttgccgcagc accgatgggc gttgaaatta
     6361 tggctcctcc aggcatggaa gagatgacca gccagctgca gtccatgttc cagaacctgg
     6421 gcggccagaa gcaaaaagcg cgtaagctga aaatcaaaga cgccatgaag ctgctgattg
     6481 aagaagaagc ggcgaaactg gtgaacccgg aagagctgaa gcaagacgct atcgacgctg
     6541 ttgagcagca cgggatcgtg tttatcgacg aaatcgacaa aatctgtaag cgcggcgagt
     6601 cttccggtcc ggatgtttct cgtgaaggcg ttcagcgtga cctgctgccg ctggtagaag
     6661 gttgcaccgt ttccaccaaa cacgggatgg tcaaaactga ccacattctg tttatcgctt
     6721 ctggcgcgtt ccagattgcg aaaccgtctg acctgatccc ggaactgcaa ggtcggctgc
     6781 caatccgcgt tgaactgcag gcactgacca ccagcgactt cgagcgtatt ctgaccgagc
     6841 cgaatgcctc tatcaccgtg cagtacaaag cactgatggc gactgaaggc gtaaatatcg
     6901 agtttaccga ctccggtatt aagcgcatcg cggaagcggc atggcaggtg aacgaatcta
     6961 ccgaaaacat cggtgctcgt cgtttacaca ctgttctgga gcgtttaatg gaagagattt
     7021 cctacgacgc cagcgattta agcggtcaaa atatcactat tgacgcagat tatgtgagca
     7081 aacatctgga tgcgttggtg gcagatgaag atctgagccg ttttatccta taatcgcgtt
     7141 caatcatttt catcattgtt tgatggggct gaaaggcccc atttttattg gcgcgtatta
     7201 tgactgaaca acaaattagc cgaactcagg cgtggctgga aagtttacga cctaaaaccc
     7261 tccccctcgc ctttgctgca attatcgtcg ggacggcgct ggcatggtgg caaggtcact
     7321 tcgatccgct ggtcgccctg ctggtactaa ttaccgccgg gctattacag atcctttcta
     7381 acctcgccaa tgattacggc gatgcggtaa aaggcagcga taaacctgac cgcattgggc
     7441 cgctacgcgg catgcaaaaa ggggtcatta cccagcaaga gatgaaacgg gcgctcatta
     7501 ttaccgtcgt gctcatctgt ctctccgggc tggcactggt tgcagtggca tgccatacgc
     7561 tggccgattt tgtcggtttc ctgattcttg gcgggttgtc gatcattgcc gctatcacct
     7621 acaccgtggg caatcgtcct tatggttata tcggtctggg tgatatttcc gtactggttt
     7681 tctttggctg gttgagcgtc atggggagct ggtatttaca ggctcataca ttgattccgg
     7741 cactgatcct tccggcgacc gcctgtggcc tgctggcaac ggcagtactg aacattaata
     7801 acctgcgtga tatcaatagc gaccgcgaaa atggcaaaaa cacgctggtg gtgcgcttag
     7861 gtgaagtgaa cgcgcgtcgt tatcatgcct gcctgctgat gggctctctg gtatgtctgg
     7921 cgctgtttaa tctcttttcg ctgcatagcc tgtggggctg gctgttcctg ctggcggcac
     7981 cattactggt gaagcaagcc cgttatgtga tgcgggaaat ggacccggtg gcgatgcgac
     8041 caatgctgga acgtactgtc aagggagcgt tactgactaa cctgctgttt gttttaggga
     8101 tattcctaag ccagtgggca gcataactga caaatatcaa ttaacaattg atgattttgc
     8161 caacagccta catagcgcga tatactgaaa attctcgcag caactgaatg ttaagcctat
     8221 gaaatacgat acttccgagc tttgtgacat ctatcaagaa gatgttaacg tcgtggaacc
     8281 gctgttctcc aactttggcg gacgggcgtc gtttggcgga caaataatca cggtaaaatg
     8341 tttcgaggac aacgggttgc tgtacgatct gctcgaacag aatggccgtg gtcgtgttct
     8401 tgtcgtcgat ggcggtggtt ctgttcgtcg cgcactggtc gatgctgaac tggcgcgtct
     8461 ggcagtacaa aatgaatggg aaggtctggt tatttacggc gcggtgcgtc aggtagatga
     8521 cctagaagag ttggatatcg gcatccaggc gatggcggca attccggttg gtgccgctgg
     8581 cgaaggcatt ggcgaaagcg atgtccgcgt caattttggc ggtgtaacct tcttctccgg
     8641 cgaccatctt tatgccgaca ataccgggat tattctttca gaagatccgc tggatattga
     8701 atgataagaa aggcaccgca aggtgccttt tttctgcgtt acctgttggc ctacacagta
     8761 aagaaattac gcgaaagatg aagcgtaatc agacctcttc catgcgaccc agcagggcct
     8821 gcagacgttc ctgccagccg ttctgctgtt ctttcagatg gttgttctca cgctccagct
     8881 cttcgcgctg atgctgggca ttttgaactt cctgcgacag tgagttgttt ttttctttca
     8941 gctcttcgat ttccatctgc aacagagtga tggtatcaat cgcctgctgt acttttgctt
     9001 ccagtttctc aaacacttct aatgacattg tcatacctct cctgaattgc aaggcgttga
     9061 tggataaaaa atcctcgtcc cgattaccgg tgacgcctta ataaatacga gcgcacttta
     9121 gttagctccg attgtatgaa gccgcgccat cgctgtccag cggcacgcct tgcagattac
     9181 ggtttgccac acttttcatc cttctcctgg tgacataatc cacatcaatc gaaaatgtta
     9241 ataaatttgt tgcgcgaatg atctaacaaa catgcatcat gtacaatcag atggaataaa
     9301 ttgcgcgata acgctcattt tatgacgaag cacacacatt ttaagttcga tatttctcgt
     9361 ttttgctcgt taacgataag tttacagcat gcctacaagc atcgtggagg tccgtgactt
     9421 tcacgcatac aacaaacatt aactcttcag gatccgatta tgagtcaaac atcaaccttg
     9481 aaaggccagt gcattgctga attcctcggt accgggttgt tgattttttt cggtgtgggt
     9541 tgcgttgcag cactaaaagt cgctggtgcg tcttttggtc agtgggaaat cagtgtcatt
     9601 tggggactgg gggtggcaat ggccatctac ctgaccgcag gggtttccgg cgcgcatctt
     9661 aatcccgctg ttaccattgc attgtggctg tttgcctgtt tcgacaagcg caaagttatt
     9721 ccttttatcg tttcacaagt tgccggcgct ttctgtgctg cggctttagt ttacgggctt
     9781 tactacaatt tatttttcga cttcgagcag actcatcaca ttgttcgcgg cagtgttgaa
     9841 agtgttgatc tggcaggcac tttctctact taccctaatc ctcatatcaa ttttgtgcag
     9901 gctttcgcag ttgagatggt gattaccgct attctgatgg ggctgatcct ggcgttaacg
     9961 gacgatggca acggtgtacc acgcggccct ttggctccct tgctgattgg tctactgatt
    10021 gcggtcattg gcgcatctat gggcccattg acgggttttg ccatgaaccc agcgcgtgac
    10081 ttcggtccga aagtctttgc ctggctggcg ggctggggca atgtcgcctt taccggcggc
    10141 agagacattc cttacttcct ggtgccgctt tttggcccta tcgttggcgc gattgtaggt
    10201 gcatttgcct accgcaaact gattggtcgc catttgcctt gcgatatctg tgttgtggaa
    10261 gaaaaggaga ccacaactcc ttcagaacaa aaagcttcgc tgtaatatga ctacgggaca
    10321 attaaacatg actgaaaaaa aatatatcgt tgcgctcgac cagggcacca ccagctcccg
    10381 cgcggtcgta atggatcacg atgccaatat cattagcgtg tcgcagcgcg aatttgagca
    10441 aatctaccca aaaccaggct gggtagaaca cgacccaatg gaaatctggg ccacccaaag
    10501 ctccacgctg gtagaagtgc tggcgaaagc cgatatcagt tccgatcaaa ttgcagctat
    10561 cggtattacg aaccagcgtg aaaccactat tgtctgggaa aaagaaaccg gcaagcctat
    10621 ctataacgcc attgtctggc agtgccgtcg taccgcagaa atctgcgagc atttaaaacg
    10681 tgacggttta gaagattata tccgcagcaa taccggtctg gtgattgacc cgtacttttc
    10741 tggcaccaaa gtgaagtgga tcctcgacca tgtggaaggc tctcgcgagc gtgcgcgtcg
    10801 tggtgaattg ctgtttggta cggttgatac gtggcttatc tggaaaatga ctcagggccg
    10861 tgtccatgtg accgattaca ccaacgcctc tcgtaccatg ttgttcaaca tccataccct
    10921 ggactgggac gacaaaatgc tggaagtgct ggatattccg cgcgagatgc tgccagaagt
    10981 gcgtcgttct tccgaagtgt acggtcagac taacattggc ggcaaaggcg gcacgcgtat
    11041 tccaatctcc gggatcgccg gtgaccagca ggccgcgctg tttggtcagt tgtgcgtgaa
    11101 agaagggatg gcgaagaaca cctatggcac tggctgcttt atgctgatga acaccggcga
    11161 gaaagcggtg aaatcagaaa acggcctgct gaccaccatc gcctgcggcc cgactggcga
    11221 agtgaactat gcgctggaag gtgcggtgtt tatggcaggc gcatccattc agtggctgcg
    11281 cgatgagatg aagttgatta acgacgccta cgattccgaa tatttcgcca ccaaagtgca
    11341 aaacaccaat ggtgtgtatg tggttccggc ctttaccggg ctgggtgcgc cgtactggga
    11401 cccgtatgcg cgcggggcga ttttcggtct gactcgtggg gtgaacgcta accacattat
    11461 acgcgcgacg ctggagtcta ttgcttatca gacgcgtgac gtgctggaag cgatgcaggc
    11521 cgactctggt atccgtctgc acgccctgcg cgtggatggt ggcgcagtag caaacaattt
    11581 cctgatgcag ttccagtccg atattctcgg cacccgcgtt gagcgcccgg aagttcgcga
    11641 agtgactgca ttgggcgcgg cctatcttgc tggtctggcg gtcggtttct ggcagaacct
    11701 cgacgagctg caagagaaag cggtgattga gcgcgagttc cgtccaggca tcgaaaccac
    11761 tgagcgtaat taccgttacg caggctggaa aaaagcggtt aaacgcgcga tggcgtggga
    11821 agaacacgac gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcccgaa
    11881 acgtgcgggg gcaaccccgc acacatcaat aatccctccc ttcccctgtg ctacacttcg
    11941 cgccattcct tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg
    12001 cgtcaccgaa tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac
    12061 cgcggacggc gcggcggtaa acgccatgcg tattatgctc aaccaggtca atattgacgg
    12121 caccatcgtc attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa
    12181 agtcggtact ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg
    12241 catgacggcg atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg
    12301 cttcctcaat gcgccggata tgtatatgga gaagctgatc gtcggaccgg gagccaaagg
    12361 caccattgat ttgaacctgc cgctggcgga taacctgcgc aatgttgcgg cggcgctcgg
    12421 caaaccgttg agcgaactga cggtaacaat tctggctaaa ccacgccacg atgccgttat
    12481 cgctgaaatg caacaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc
    12541 ctcaattctc acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc
    12601 gccggaaggc gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg
    12661 tctgctggcg cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca
    12721 ggagctggca cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga
    12781 tatggcgcgc agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct
    12841 ggaaggcatt agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa
    12901 gtcacgcacc attcgccgca ttcagtctat ccactatctg gatcgcaagg acccggaaat
    12961 gcaggtgcac atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga
    13021 atttttttgt tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc
    13081 tgattgggta acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct
    13141 caccgttcac gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga
    13201 aatcgacggc gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga
    13261 tctggagttt tacctggtca ccgtccccga tggcaaatta agcccacgac tggcggcact
    13321 gaaaccaggc gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggagga
    13381 agtaccagat tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt
    13441 atcgattctg caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc
    13501 cgcacgttat gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta
    13561 cgaaggcaaa ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac
    13621 cggacggata ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat
    13681 gaataaagaa accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca
    13741 acagttgctg aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat
    13801 gacagcggag cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc
    13861 gcactcatct tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtgg
    13921 tgatgaccac agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc
    13981 ggcatttctc cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt
    14041 ttatacagct tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg
    14101 aagtagatac ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga
    14161 gcgtcattat gtctggcgag ctccagggct ttattcacca gtaaggcatc ttcttcattc
    14221 ccggaaattg ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc
    14281 atcaagctaa cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag
    14341 tgaaggccgc atcaagctca acttgcccct ccagatcctt aatcttatag gccacgctgg
    14401 ccccttcatc tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact
    14461 ctgcttcttc gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa
    14521 tcaccgtacc gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca
    14581 tagggcacct ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta
    14641 agggaagaac aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg
    14701 tgaagagttc agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg
    14761 gaaaaaaata gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta
    14821 tacttcacat cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca
    14881 ccaagatcga taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat
    14941 tgccagacac ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc
    15001 agaaatgccc atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct
    15061 gttggccaaa gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta
    15121 cccgccagtg agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa
    15181 tcacggcgtc caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt
    15241 ccttgatggt tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt
    15301 aatcggagcc agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt
    15361 ctcttatgtt ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca
    15421 acgaccgcgc cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt
    15481 cgagaagact ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac
    15541 agcagtcccg acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat
    15601 gcttctacag cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta
    15661 cccatccagg ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca
    15721 gtgatccgca cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg
    15781 ctactaacgg cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat
    15841 gttgtcgcgg acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg
    15901 ctatctgaat cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga
    15961 atctctatac tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct
    16021 tataagcgtg gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg
    16081 gcagccgcca catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg
    16141 ctggctgtgc ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag
    16201 ctgaaggcag ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat
    16261 tcaccggtga aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg
    16321 gccactctga acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg
    16381 cggtgctgaa agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa
    16441 acgaagcggg caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc
    16501 agggtgctgc ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta
    16561 ctggcaaatc tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca
    16621 tcgctaaagt tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa
    16681 acgcgtctaa cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg
    16741 gtgcttctct gaaagctgac gctttcgcag tgatcgttaa agctgcagaa gcggctaaac
    16801 aggcttaagt ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg
    16861 ccttaacgca aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc
    16921 gtcagtagat ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc
    16981 gccagcccgt agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata
    17041 aatgggctgc gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg
    17101 cgcaaaccac ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt
    17161 tgttcgcgca catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc
    17221 ccggtgcggg agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt
    17281 ttgctcataa aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg
    17341 gtcaacaaag gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt
    17401 ggaccattaa gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc
    17461 gaaggatttt gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat
    17521 gtatccgact cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata
    17581 actatcatca ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa
    17641 gagatgcaag ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg
    17701 ggtttgataa ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt
    17761 agcaaaatgc tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa
    17821 cttcagcttt ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta
    17881 gtagtttttc gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc
    17941 cgccacctct ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc
    18001 tgcgaggata gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc
    18061 tgccagcaga gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa
    18121 ggtgttagtg gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg
    18181 aacaaaatcc tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc
    18241 agccaggtag ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac
    18301 acccggttta atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag
    18361 gaaaacaatg gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt
    18421 atcaatccgc ccgcgttccg caatcgcgtc cacgtcatag gccagagcca gcgtgacaac
    18481 atcagcttca ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg
    18541 acgaatcacc acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta
    18601 ctgttcgtac aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc
    18661 cataacgctg gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg
    18721 actctcttat gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag
    18781 aataaaaaaa gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga
    18841 aatccgcctc atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc
    18901 ttatccggcc tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata
    18961 agcgaagcgc atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct
    19021 ttttccgaaa tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac
    19081 gcttcatgtt ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac
    19141 ctacacaacg accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg
    19201 aagccagaat acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag
    19261 ttgcgcgggt ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac
    19321 acatatgttc ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga
    19381 tttcgtttac caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc
    19441 caccggcaat ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca
    19501 cggaaatacg ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg
    19561 tgctcagcgc agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag
    19621 tgcccggcag accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg
    19681 aaccgtcacc gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag
    19741 ccacggcgcg gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac
    19801 cgccacggtt gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat
    19861 acagacccag atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac
    19921 gaacaacccc gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac
    19981 cgattttctt aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg
    20041 aagtcttctt gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc
    20101 tgctgaattg attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg
    20161 ctctctttta tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac
    20221 aggggtcctg atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta
    20281 cctgatccgc caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa
    20341 tctgaataaa gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac
    20401 ccggccagga agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat
    20461 ccagtaatga ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc
    20521 cgattcccaa tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg
    20581 ccagcagaat tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca
    20641 ccgcctggct ttgcgtccgg cgcaccaccc accgctgaaa cgagacaagg ataatcgtac
    20701 aaattagcgc cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag
    20761 ataccagatg ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac
    20821 tttgcgccag cgccgcgagg gactcagctt taccgtgacc aaacgagtga ttatcgtcgg
    20881 caggttgcag ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca
    20941 gcgaatccac cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt
    21001 taatcagcaa tagcagcgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc
    21061 gtccataaga ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca
    21121 aatcatctgc aatgcattaa gcagcaggca aattgaggat aaaaaaaccc ccacatcatg
    21181 tgggggaaga cagggatggt gtcacaaaaa agtacctaac ctgatgatat aaatggattt
    21241 atgtcaaccg ctgtccacat agcgtccaca tcgaccataa ataagcccct caactgaggg
    21301 gctatttttt tgatcacatc cacataattt gctgccctga cggcaacggg tgcggcctta
    21361 cggcgtggac ttctcccggc ttcacgatgt atcgctgtac cgactcataa gtgatgaacg
    21421 tggcgctgca attcacgttc tggcactggt gataacgctc ttttgtcgtg tcagtgatat
    21481 agcgacttgt acgcgcatgt gcggcatgct ggcataaagg acaatgaaac atcgcgagca
    21541 cctcttccgg ttttgttgat ggtgccattt tagttaattt atccttataa aacaaacaga
    21601 taaataaacc acgtcactca tcatcttctg tttcgtactc cacatcagaa agccttacct
    21661 caagctccaa ggacgtcgtg aagccgctat tattgagaca atgtgtcacc ttagtgatgg
    21721 tccagtcctg ctcgtctatg acgcgcttaa agcctgacac tttaaccggt gtttccgtgt
    21781 aaatatctgc acgaccggta gccaggctga tggagaactc cgctacaccc cgttgcagtt
    21841 tatcccactt cgcctgagcg gcgcgcatgg cctgcgcttt tgtggcatat accgtggtca
    21901 gggcaaaaac gttgtcagct tcaccggcca tgtattcacc ttcgcgcgct tccggtactt
    21961 ttggcgcttt cttctgcgtg accggtttcg ctttcgggtg ctccagtgcg cgcaggtgtt
    22021 tttctttctt tttgcgtttc agttttacct tctgcttttg cggcttcggg tctttggtgt
    22081 gtaaccactt tgccgttacg ccggtgtaag ctccacggtc agcaatcgca aaatgatgac
    22141 ggtcgccgtc gctgcgggtg atggtaatct gcgggatttt tttaccgctg gccgtcaccc
    22201 cctgccccgc tttgagaaac agcagttttc ccatttttac cgacacctcg ccgccgttgc
    22261 gttcagcaag acgggtcagg aatttcgcat cagactcctg cgactggtcg atgtgtggaa
    22321 tttttattcc ggccagtgac ggagcgacac tggcttccag cctgttacgg gaggctatcg
    22381 cctcaacaat cgcaccgagc gtaatgtcat gccatgagcc ttcacggcgg gaattgagcg
    22441 tcccgcgaaa atctgcactc cgggcgcgga tggtgaccac atccggcgcg ccccggtgtt
    22501 caacctcatc aacggtaaat ttccctttgc ataccagggc aaaacctttc cagccgatat
    22561 acaccgtcag gacagcgcca cgaaccggca gcccgacctg cccgtcggca tcgttcagtt
    22621 caatatcaag ctggtcagcc tcaaagcccc ggttatccgt cagggtcatg ctcatcagac
    22681 ggtcgctgat attgccggta atatccctgc tgtcgagcat cagcatgtaa tccggcgtca
    22741 gcgtactgcc tgcatcaaat gtcagcgcat ccagcattat cccgcccccg tcatacccgt
    22801 gaatttagtc gccatactgc cagccttacc gatgagcgat tccgcctgtt taccgatatc
    22861 gccataaagc gcggccagtg actcatcaac gcgggtgagc gacagcgtaa aatcaatttt
    22921 ccgtggtgtg ccgtctgcaa agaaaatact ccctgtttca ctcaccttgc tgatgacata
    22981 catgccgtaa atcatgccgg tgccatccag caacggccac gcccggcctt cctctgccat
    23041 cagtctgagc gtggtcatcg tcagctttcc gccagtcagt tcgggataaa gcacaccggc
    23101 aagtgtgatg ttctcctcac ctacaccgag aaactgatag gcatcccgtt taccgatacg
    23161 ggaatttgac ggccagcgat aatctgattc acgctgcatg gtctggtgtg gcagcgtctg
    23221 gcgcataaaa acaaacatac ctaacgcgag catcattttt cgtcacctcc ttaaccgtca
    23281 tgcatcatgc tggcacgggc gcgcgcacgt ttatcccgct cgtatttttc gagcgcatcc
    23341 tgtaactggc ggtcaagctg tgtccccggt gcagtaccac ccgtcaggct gatgtgatat
    23401 tcgtttttac tctggtccac ataagagcgg ccagccggtg ccgtgaccgg ctgataagcc
    23461 tgatagcctg cataagagct ggtcgccgga atataaccac cggtgccata cgtggcggca
    23521 tgagttctgg cggcggtctg gtcaagtgtg tctgactctt tgttgatgac cccgagcttt
    23581 tccagtaccc agtcaatgcc gctgcgcaat ttgttgaacg cattaagcgg cagcatcagc
    23641 gcgtcagcca gtgcctgccc gaacatgacg cccgtgtcac ggcaacggtt cagggtgtcc
    23701 tgggtggctt tgaccggggc aatcaggttt ttaaaccact gccacgcagc ctgtaacttt
    23761 tcgcccagcc agtcaaacac cggtttcagt ggcgtgaaca gctcccccac cggcgcaaat
    23821 gccgctttca gcccttcaac cacaccgcca aagaatgcgc tgacaggctc ccagtattta
    23881 cggataagca acgccccggc gacaatggcg gccaccacag ccacaaccgg ccagctaatc
    23941 gccccgatgg ccgtcataac ggcactacca accgtcgtga agattgcccc cattgcgcct
    24001 gctgccgcga tgatggcatt gatgccggtg ataaccggcc aggctacaag accaattgca
    24061 ccgatgatgc cagtcagcgc cagcgcgcca ccggcaatga ggccaatggt tgacgccagt
    24121 gatttgtttt tctgtaccca gccgtcgagt tttaacacat actttgtggc cgtctgcgtg
    24181 agcttacgta gcgcgccttc ctgctggtca aacaggtctg tccccaccgc ctcataagcg
    24241 gactgaaact ccttaaagtc accgccgagg ttgtcctgca tgatatttac cagctctgcg
    24301 gtcttcccgt ctgaggcttt aaacgcagcg gtcagtttgt ccagctttcc ggttgacgcg
    24361 gcagtcatca gcacagcggc ggctgagctg gcctcctccc cgaaaatggt tttcatgtat
    24421 tcggcctgct gggcagtacc gagccggttt ttctcaaagc tggcctgcat ttctttcaga
    24481 atggtaaata ctggtcgggt gtttccctta ctgtctgagg ttttcacgcc aagctctttg
    24541 agtgcatccc atgcttttcc agtcggtgcc tgcaggcgac ttaacacggc acggcttccc
    24601 gtccccgcca ttgagcctgt gatttttgca tcatgcagcg ccccgaccat tgcggcggtt
    24661 tcttcaatgc tgacaccggc attttttgcc actggtgcgg cataggtcag cgcatcgctc
    24721 atgccgtcaa aatcagcggc ggttttgttc atcgtcatgg agagaacatc cccgatatga
    24781 gcgaccttat cgtttgaaag ctgaaaggcg gatttcatcc ccatcagcag ggcggcgttt
    24841 tcttccattg tgcggcggtt cgccagcgcc atattcagcg tgaccggcgt tgccgcctga
    24901 atggcatcaa catccccacc cgctttcgca atgataatct gtgcaccggc cgcatcatcc
    24961 gctgaggcgg cggtgttgtc accgagctgg cgcgcctgct tgcggagtgc agccatttcg
    25021 gcggagtctt ttgccacacc gagcacagcc tgcaattctg agtttttctg cgcaaactca
    25081 taacctggca tcagcagctt aactccggct atcgttcccg cagcagcaat ccccacaccg
    25141 gcagcgccca ctgaggccat atttccggcc agttcctttc cggcctgata acgctgtttg
    25201 actgcgttaa gttttgcctg ttgcgcactg acacgcgcca gcgcgtcacg ctgccggtta
    25261 agctgggcgg tggtttcact gatacggttt ttcagtccct gctcatcatg tgcaagattg
    25321 cgggtattaa ttcccacagc ggccagttcc cgctgctggc gtttaacgga atctgtcagg
    25381 cggttatatt tcgcctgtaa gtcctccgcc gcacgctttg cggattccag cactttcgcc
    25441 tgagcacggg tcgggcgttc ggtgttttta aactgtgtgg caagggcttc ggcctcctgc
    25501 cgtgcctttt caagtgcatg accagtcacg gcgagctgtg cactggtctt gcggaatccc
    25561 tcaatacggg atgcgtgacc gttcagctcg cgcagtgatt tttgtgtttc ccggatatcc
    25621 cccgacagca acttactcgc tctgcggatg gatttaaacg ggcgggacgc ctggtcaaca
    25681 gccctgagca atacctgtaa ttttacattg ttactcattc gtgtttccgc ttcgcctgag
    25741 cgccttttcg cgccatgtga tgagttcggt caggctcatg ggatacagtt ctgatggcgg
    25801 ccagtgaaat atcactgcca catccgccat caggtcatcg accgacagat ttttcggaag
    25861 cgtcactgca ccgagttcgg cgacaaaaaa ccgaccacct taccggccag cgccacaagg
    25921 tcaggcagtt ccagcgcggc gacttcctgc tcggtcagca tcggtgccgt catgcgcggc
    25981 agcaccttaa tcagtgcatc gacttctgag tttgcgaccg cagccagact gacaccgcgc
    26041 agcgtccccg cactgggttt catcagcgtg acctgttcga taacctgctc accacgcttg
    26101 accggattgt ccagggtaat cacattttct ttgttcatgg ttttctcact tctgaatcgg
    26161 ggttaaccgg tcagccaggc tgaccggatg aaaattacag gccgatattg cggcggtgtt
    26221 gctccagccg gtcgacgccg ttcaccttct caatcatgtt gatggtgtcg atttcgacca
    26281 gctccttacc gtccatcgtc agccggaaat aggtgcagac cacggagatt ttcgactcgg
    26341 tgtcttctcc ctgtttaccc tcgccggtgt cgatttcttt ctgacggcca cgcatgacca
    26401 cctctacggc caccgtttcg ccggtatcgt cacgctggta agagcctgca aaacgaatcg
    26461 gcacggcatc cacaccggtt gcggcgtaaa gctcccagat aaccgaatcc gggaagcccc
    26521 cgagcgacca ctccattgac agcgcatcgt catcaaggcc gaggtctacc ggtgcgcttc
    26581 cgttcatccc cgcaccgcga tagttttcga gtttacgggt cagttttggc agcgtgacgg
    26641 actttgcaac gccctgatag ctgtagccgt tcagaaagac gttcattaac ttgagtttgc
    26701 gcggcattgc catcggtcag gctccttaat tgctgttaac cgaggtgacc agatttgcca
    26761 ggtatttatc ggtaatacgc tggcgcaggg tcaggttttc aagaggaggc accggggtat
    26821 agtcgtagtc gatatacagt tttccggcct tgagggtttc cgcatcgttg gattcttcgc
    26881 tgaaccagca ggtcgcatcc acgatatagc cgtttgtttt cagctcacgg aatttggcat
    26941 tgatgccgtc aacgatgtcg cgaatcagcg ttgcggtgat gggcttgtcc accgcccaca
    27001 tgtgcgcctc agccatcgtg tcggccagca cctgcgcggt gcgggtgtag ttttcaaaga
    27061 ggaacagcgg gtcatcagag caggtacggt taccccagaa gcggaaaccg tcacggcgaa
    27121 tcagcgttgt gacgcctgac tcgttaagca ggtcagcatc ggtgccggac tcctgcaaat
    27181 cccagaatac ggacgcgctg atgccggtaa caccgttcac cccgacgttg gacagcgttt
    27241 tatgccagcc ctgctcctgg tcgattttag cgcgcagacc cagcgcacgg gcggtggcat
    27301 acgcggtggc ggtggtactg gcgaccgtat cccatgcgag gaaatccggc cagatgacca
    27361 tcagctcacg ctggctgaaa ttctgacggt aggctttcac ctcggaaacg gtcttacagc
    27421 cccatgcgct gatatacccg aaagcgcgca gcttctgaca gactgatgcc agtgcaacag
    27481 ccacctcttt ggtgtccagt cccggcacgc cgagaatacg cggtttaaca ccggttaccg
    27541 actccgccgc cagcagggct ttcagtccgg tgtactgacc gttttcgtcg gtggtgccga
    27601 tgatattgga aacggtctgc gcgagtttcg tttcctcgtc gtcgccggtg ccgtcttcca
    27661 cacgcacgac aacggtgacc ggttttgact ggtcggcgat ggcctgcaac gatgccgcca
    27721 gcgtgccttt tttaccggcc tttgcaattg cgctctgcac attggtaatc agcaccggtt
    27781 tattgagggg gaaggtttcc gcatccgcat cgctggccgt gcagaccatg ccgacaatgg
    27841 cagtggatac ggtggaaatg acgcgggtgc cgtcgttaat ctccagcacc tgcacgccgt
    27901 gatgatagtc actcatccgt ttaactccgt ggttaatggg tgcaactatt ttctgttggg
    27961 cagtgcatga gacgctattt gacctggctg gtcagtggat gaaacaacag ataaagaaaa
    28021 tgcgggcaat tcgcccgcat gtcctgattt gtacacactc attttccgac tgacagttta
    28081 catagcccaa acgctatcaa atctgacagc ctgctatgag cgaaaagcgt taaatctcac
    28141 gatatctgct accgggaaaa atttcgatta tcttcgatgg gaactttgat aatgtagtaa
    28201 atgccatttt cagcgatcac ttttcaagcg tgtgtaaatg attaatgtta aaaaattgac
    28261 ttcactgaaa taaggttgct agtatttttg gagcaaccat taatgttttt tatttaagga
    28321 atgtaaatga catacgcact gattgataat tcaactctaa ctgcagtcca aagaatcgag
    28381 gggaaagtaa aaacaagatc gaaggactca gtagatacag acataatagc atttgagaac
    28441 tatatacagt ctattttgtt ctatgatcac gttatcgcgg ttgatgatta tattccagag
    28501 taccgtggga gtaggatttc aagtttcaga gagatattat ttcttgataa agcacaatat
    28561 gggttggatg aaattgaaag tgaagcaaaa aaaatatctg atgcattgaa acctaaaatt
    28621 cgtggtggaa catttgttaa tgacgatttt aaaaaactaa ttgaacttct tcagacacat
    28681 atcatatgta catgggacat tagttcaagt gtttatcatc taacactgaa gaacttatct
    28741 gatggaggta aggagtttga aaaatatggt aacattgctg ctgccatttt ttcagaactg
    28801 cgtgatgctg ctgagtctgg taatagaaca aatggcgatg ttgaacttct cgatcgattt
    28861 ggcaacccta ttcgcaaggg gtacaaagtt cccggagcaa aatgggggga tggcacatct
    28921 gctggtgaag catcagatgc aatcaaagca ttcgtggctt cgcttgtatg gctggctaat
    28981 cgggcagtat tttattctct cacagctaag taccttcaag cagatacttt tttatatcca
    29041 ttacgtcagg cgtatcaaca gacatattta agccaaaact gtcagtatgg ttttgactat
    29101 gctaaacata ttgtagaaaa cttttccact tcgttaagtc atgatctcat tgatattcat
    29161 tctggtggta aagctgtagc aacagcaact tcgcttcctt tattttcggc atggctcgca
    29221 aaagaaactg gtgatccaag ttgtattgtt gaagctgctt ataacataag ggacaacaac
    29281 gaatttgtaa gggcccgtga gaaaatgcga gaaataaagc gcttgtttga tgaaaatgaa
    29341 ttgcctcaag ctaataagtc agtaagagca ttgcttactg aagtatcgaa agaatcagat
    29401 tatattcgaa ctaagtatgg gctcaagact agacaaggtg tacctgtaac taagcttgta
    29461 catgtataca atactgttgc ggctattaat tcccttccga aactaccgac ctttaatttt
    29521 aaagttaaag ttcctgattt cttatatgat cttaaaaaac ccaaggggtt tcgtgctctc
    29581 tatcgaaatt tgactaatga tctttctact gtgtggtcgt taggagaagc tcgagatatt
    29641 cttggaagta aagttgtgat agataatcat gcaagaattt acagtccgaa gcatgaagaa
    29701 ccgcgtttca aaaactctca ttccccatat aagagcccga tgtagcttaa gaaaaagaca
    29761 cgcccacctt gtgcgtctac ttctggcaca gagcggactc tcagattagg ctttactctg
    29821 tgccatagat atgtaagctc acaccagagc tcatacaact tattgcggca tttccggcca
    29881 ttcaggattt gcaggatcca cacgactgac cagaacactg tagcgttccc atgcctccag
    29941 tcgactgcgc tcctcatctg ttgccatatt cagcctgaca gcgcgctcca gcggcaaaat
    30001 cacggattca gcatctgcaa gaagtctggc tttccgattt tctgcctgct gctgcaattc
    30061 ctctgccgta taaattcgtt taatcactgt acagtcctta aacatccagt tccctgaaat
    30121 gtccgcccgt cggttagcag taatatccgg cacttcaaca acacttaatc catccggtct
    30181 gatagctgtc acatcctttt ccacatagcg gataatatta tctttgtcgt acgctatttt
    30241 tatcgtgtca tcagcaaaat acttttgttc ttcgtaccag ttcttaccat cttctgaaaa
    30301 aaaccagaca acatcaaagt cctttgtcaa ttgatattgt tcaaccgttt ttggattacc
    30361 tgccgttata tttatcaaat gctgcataaa ttatacctgt gccacgttat accaagtccc
    30421 gttaatgtat ttctgcaccg gtctgtaata tacgccacca atgttatcgg cagagtttga
    30481 gccggtatcc tgaacaataa tgccggaata tacacacccg gacggtgcct gatgtgtcca
    30541 tgtcatacca ttgttcgcag gtttgtatgt ggcagcacca ccaagccgga tatcccggac
    30601 atagcgtgaa tcaaagttgc catagttaga tggtgatacc tgcccgttaa cagcaaaagt
    30661 gatgctgtta tctgtatttc tctgactgta aaaatgccag cctgcatcat cacctaattc
    30721 agccaccaca ggacgacttg agtttcccca caaattgaat gcggcttcct tcgtggatgt
    30781 attgctgctg ctgaccgtga actttttccc gctaccggca cgtactttgg tacttgagac
    30841 aatatcacct gtaacactca ggccatgccc cattgacact ccgccattgg cgttattgat
    30901 agtcagcggt cttaagccgt tccatgtccc aaatttatcg ccagaggccg tcagcattaa
    30961 atatgtgctg ccgccatcat tcctgataaa gaatccataa ttgccataag caatgcgcag
    31021 accattagca ctgagtgatg taatttcacc tcttgaacgg agaccataag cggagctgag
    31081 tgataattct tcctgagcgt cattgtttcc tgtcgcccag cgaattaccc cgccctgaac
    31141 tgtttcatgc cagatagtat ctccttctcc accacgaaac tttctgagat attttttgcc
    31201 gcctctggtg cctgaacata aggccgtaga catataggca ttctggcttc cgccatcctg
    31261 attaatcgtt ccggtcattg agtcgccctg acgattccag tcacgacgcc agccggggga
    31321 gtagccgtcc ccatgattta tgtaagtgaa ttgcgcgctg gttgtaccgc caccgcttga
    31381 tgttgttggc gtggtcactc ggatagtgat tgcagattta gttcccatga cctcgacgac
    31441 acaaccagcc aggtggatat caccacatcc ggtatccgta atgattttgt tatttgcata
    31501 tgaccaggag cctttgcaca tccagtacgg atgattaaat gcaccacggg aatccagcca
    31561 ttcaataaac tgagcggttg tccagtttcc ggcttcagtg ctcaaagcgc cgctataagc
    31621 acgacaggca ccgatatttt tcgtgaaggt atcctttccc ggaatatccg caccgttctg
    31681 atctttctga agacgttttt cagcattgtc ataggcagac ttcactgctt ttggcgttgc
    31741 ggccagcgtt tcagaatcgc tgttgatggc actacttagc tggacaagac cttttcgcgc
    31801 tgtggtggca tcctgtgcag tgtatttccc gttagcaagg tcatacgctg tcttaaccgc
    31861 ctttggtgtt gccgcaagcg tttcagaatc gctgttggtg gcgctactga gctggacaag
    31921 accttttcgc gctgtagtgg cgtcctgtgc agtgtatttc ccgttagcaa ggtcatacgc
    31981 tgtcttaacc gcctttggcg ttgccgcaag cgtttcagac gtgctgttgg tggcactact
    32041 gagctggaca agaccttttc gagctgtggt agcgtcctgt gcggtatatt tcccgttagc
    32101 aaggtcatag gcggccttta ccgctttcgg cgttgcggcc agtgtttcag acgtgctgtt
    32161 ggtcgcactg cttaactgag taaaaccttt tgcggtcagc gaggcgtccg ggtgacgtcg
    32221 tgactgttcg tgctctgcaa ttttgtcatc aacgtaatcc tgtgtcgcca tcaccgttgt
    32281 ggtgtcaatg gtcagctcca ccgaggccac actgctgacg atgatgacca tgcgacaggt
    32341 ctgcgaacgc cctgagcctt cggcaagagc tggcttataa ctttcggcca tgttcgccac
    32401 ggcaattaac gttcccgcat catcgtacag gccaagctca cgcatccaga aaccgcccac
    32461 ctccggcgga ataaccagct ctgcgataat ataattactg tttcgtttgt cctggctgat
    32521 tttgttcagc gtatgtcgcc agacttcgtg gataagcccg gtctgtccgg catccgggac
    32581 aggcaattta ccaccgccat ccccgacggc catcgtggta atgttgacct tccgccctcc
    32641 cggtgcggtt gccgctgcca gctttgctgc accggcagtg gtgataacgg ttctgaattt
    32701 tgtgctcatt attcctcact tatccggggt aaaccgtaat tacatcgccg tcgtaagcca
    32761 caccaccggc gaacaggtag ccgggaatgt cccgggtaat gttcaggcca ataagatgac
    32821 ggcttgcagg tttggcatca gcaataagcc gttccatttc ctgatacatt gcctctgtga
    32881 tgccgctttc cagtacacca atatcaagcc ggaaggtgcc gggcgggtca ctgttttccc
    32941 accactccgt cacgttgatg agatagccga gcggctccac cacacgccgg attgcgccga
    33001 tagtgccttt atgacagtgg atgaaatagg catcgcggat aacggcgcgt tttgtcgctt
    33061 ccggccactt ttcatcccac ctgtcgaccg aaaacgccca cgccagccac ggcagcagat
    33121 ttgccggaca ggtatccggg ttccacagtt cacgaatcct gaccggcgtt ttttcaattt
    33181 ccgcacaggc ttttgcggcg gcaacttcaa gcggtgatga gccggtcggc agcagtcgcg
    33241 aatcactcat ccgagcctcc ggtcacgacg cggtattcgg tacagaaaga cgcctgcgta
    33301 ctgttgagca cgatgtcggc cagcggtgca gtcagttcga cacgctgcac gccttccaca
    33361 tgcaaagcgg cataaatggc agacagacgg atgtcgcgcc ccagccggtg ctgtgccgtg
    33421 atgtacgctt ccagtttttt cacggcagca gcgcgtatgg gttcgctttc gggaccaggg
    33481 taaaggtaaa gcgtggcgtt tatctggtat tcaacgatgg cggcagactg cacggtcacg
    33541 cggtcggcca ccggcctgac gtcctcacca ttaagggcgt tacgcaccac cgccagcagg
    33601 tcttcggatg cgacaccgtt attttcacgt gacagcacag agatggtgac gcaggccgga
    33661 gacggactgg ttacagaaat atccgcgaca cgcccgtcgg cactgcgacc atgatactga
    33721 taggccccca ccgacccggc gacgcttaaa ccttcaaacg cctgctgaat acgcagacga
    33781 taatccgtgt cagattccat cactgccggt gtcggcggga gggtcgaatc atctgccggg
    33841 gtgataatca ggcgcgtggt gttgtaattg gcaccaatca catcaaggtc attaccggcg
    33901 gcacaggcca gcattaccgc ccgtgcggcc tcattcacac gctgacgcca gataagctca
    33961 cgataagcat tttcctccag cagtttgacg agaggctcgg attccagcgt cagggtacgg
    34021 gcgaccgcct cctgctggtc ttccgggtaa agggaaatca gtgtcgcctt gcgttcggca
    34081 agaatggttt caaagtccag ctcctcgacc acatccggtg cgggtagctg gttcaggtcg
    34141 ataatcggca tggtttcaac tcacagggat ggttaacgaa agtggctggc cggtgtcgtt
    34201 gtgctggccg gttaacgtga ccgtcattcg cccgtcaaag ctgcgcgccg tggtgacgga
    34261 tgacagggtg acgcgggctt cccatttcag cacggccatg taacaggcga ccttaatctg
    34321 caactcaagc gccggggtct gcggctggtc aatcattgac gccagcaacg agccgtaatc
    34381 acgacgcatc acccgtgagc cgaccggcgt gcgcaggata tcgccgatac tctggctgat
    34441 atgctcaagg tcagagacag tcaggccatc actgcgattc attccgagat aacgcgctgt
    34501 catagaggac tcccggttgt gccgccgctg tcgccggggt gtttatgggt atgcagtacc
    34561 ttcccgtttg atgagagttc accgccggtg tgttcaatgt tgccgcgcat cgtcccgccc
    34621 ttctgcactt ccagcgtgcc agtgatcagc ttgtttgtgc agaccacctc cggcgtgtcc
    34681 agagtgacgc gggttgatgc tttcaccgtg accaccggca ccgtggcagt aacagaatca
    34741 gaagccgtca cgctggccgt tttaattccg cttaccgtga gtgcactggt ttcaggttca
    34801 tactcaatca ccgccccgtc agggaaacgg atatgcaggg catccgccga cgcagacggc
    34861 gcggggttat caccggaata aatccccggc agaacgaacg ccgtgtcgag ttcaccgcct
    34921 acggccagaa tcagcacctg ttcccccacg gaaggtgccc accatgtgcg cgaacgtccg
    34981 gcgcgatggg tcagccactg aagccagtcg gtgcacatgc cgccggtctg cacacggcag
    35041 cgaccggcat taaggtcggt ttcgacgata atgccggtgc ggatcatgtt gcgcagtgcg
    35101 cgcgcgagtt cctgaatatt tgcgagagtg ttcataacgg gaaggatgcc gccgggtcat
    35161 accggcggca atgtgacgat gaggtgtcag gaatggcaca actaacggtc gaggtgagcc
    35221 aggataatct cttcaatcat ctgcacatcc tcaccggtaa agccgagcag gggacgcgcc
    35281 ggataatgaa ttttcttacc gtctttccgg gtttcttccg acagaccgaa ctgatgcaca
    35341 ctggctattt tcggtgactt cccgccgtaa aactccattg atgcctgttc agggctggcg
    35401 cggatatgca aaaaacgact ggtgataagt ttcgcaaaca tttttcgctt aacgcgaccg
    35461 gtcttttttc tggcgctctg ctgctggcgt ggtgcgtagg gtgtgccgtc cggggctttc
    35521 tgtgccatca cccgacgctg ctgactctga cgcagacgtt tcgccagttc ggcgctcagt
    35581 cgccgacgcc ctgacggtga cagcgactca ataagtccgg tcagcctgtc ttcaaaacgc
    35641 ttaaactcat tcatcccact tactcaccag ttcgccattg atataaagct ccatcgggcg
    35701 ggtgaccggc tccggcggcg gaggttccgg gatattcttc acatgcagcg cgccgtccac
    35761 ctcactgacc agcgtgcgct cggtcagcat caggctgatg ctgatatcaa agctgctgtc
    35821 attgttgatg tctgcataaa acgtgaagcc ctttttctgg ccttcgtcgg tggtcatgat
    35881 gtcgggctga ttttcccgca gccacgccag caccggcacg atgagcaggt caaaatcacc
    35941 ggtaaagtcg gtcacaatca cgttgagcgt gtaacgcttt tcgaatgaca gcgacgtcgc
    36001 cagtgtggag gcaatactcc cgttatccac gaatatccgc agcatctcgg ggcttgtttt
    36061 cagcaccgtg acggcatcag tcagcgccct gcgcaggctg tcgggtttga gcatcgtttt
    36121 cgtcctgaca gtgtttaatc atttttacct ggctggcaca gcgtgccagc gcgttctcaa
    36181 gctgccggat atcagcactt aaatcgccgt tcgtctgcgg gtcactgccc ggcatcgggc
    36241 aaaggctcac tttcgggcag gcgttgtgga caatcactgg cgtctgcgca ggctgtgcgc
    36301 tggtgcaacc ggcgcacagc atcaggcagg tcagcaccgt accagcggcg aaaatcttcg
    36361 ttttcattaa gtaacctcgt gatggttttc tcgcgctgag cttcacgctt cgcggcgttc
    36421 tccagttcct gacgcagtgc cacctgcgcc agctcgtttt tgtctgccct ggtgagggca
    36481 acatgaagct ggtttttcag catggtgatg gtcgtctgct gttcactggc gacgttgttc
    36541 gccctgtcca gcaaggcgcg caggctggca tttttgtgtt tcaccagaaa cagaccggcc
    36601 accgccagcg ataacaacac aaccagcaca atcatcagtt ttgacatggt tcccgcccct
    36661 caagacgctg acggcaggcc gtacgtatca gccggaaaaa cagcgatgcc acgagataaa
    36721 tcagtgcggt aaaaatccac ccggcagcga ccagcgagat aaacgtcgcc accatcacca
    36781 tcagagccgc tgcccgtctg cgccacggca ccggctgcaa aaacagcgac gtgacaatct
    36841 tcacggccag cgattccggc ggcagctccc gcccgtagcg ttccagcaca tactccgcgg
    36901 catacacgcc gacaccaccg gcaatcacac agataaccgt cgccagaatc gcccatgcag
    36961 cgacaaaact gacggccacg ctctgcgggt aaatcaggga cagtgccagc atcagcgcca
    37021 gcgacacgtt cagcatcagt gaaagggata atttcttcat ggtgtttact ccgtttaagc
    37081 cggtacgccg ccagcggtgc gccagacggt gaccagtttt tccagtgaat gctcacgctg
    37141 accgtaaccg gcacccggca gggacgccca gatattgcga cagcgtgaaa tggcgcgctc
    37201 aatgcgtccc gcccggatgt catccagtgc accgcgttcg cggatcaact gaatggcgag
    37261 cctgtcctgt gacaacggac tgaaatccgg cagggcaagc tgtttgcggt agtgcggcca
    37321 gaacaggtaa agctgctgat agcgaccgga ggccgtggat ttttcaccgc gacggttaaa
    37381 caccttcgcc ggtcggccat gtgcgaacgg gtggtcactg tagtcggtga agatttccgg
    37441 cttcccgtcc agtccggtga ctatcacgtc atagccccgg tttttcgtca gcggatgatt
    37501 cgccgtccct tcggacacgg ccagcatgtc gagaaaggcg gcgatattct gatgtgtgct
    37561 aattaccggc attacggttt ccccctgccc ttaaagcggc gctgaatggc aatctcaatc
    37621 acctgataac cggcgatacc cagcatggag ccgatgccgc acaccgcagg cagtgacagg
    37681 tcaggaaact gcaccagaac aacaccggca accatcgaga caaaaccacc gagcaacatg
    37741 cgcccgataa acagacgcgg agtgatgggt tcaccaccgg caagcacctt gccgacaaca
    37801 atcagcaccc caatcatgaa aagcgacagg acgctttttt cttctgctgt catgcgttac
    37861 tcccacagat tgacagtttc agccacgggc gcggtctgaa cgtcgggcag ttcgacggcg
    37921 gtgccgtgtg gcagcaccgc ccccagttca gccagtcccg gatttgcggc gagcacggcc
    37981 tcgaccacgc cctcagtgcg cccgtaatac cgaacacaaa tggcgtcgag cgtgtcgccc
    38041 tgtagcgcaa aggtcttcat cagatttgac tcacgatgca gcgcggcttg tcctggatac
    38101 gcgccaccgc ccagcgcata tcccgccaca gctcatcaat ggtgctgtca atgctgtcgg
    38161 ccttcttgtc gcccttggca ctggcatcca cgccgcggta acgctcataa agcgacgcgg
    38221 tcgccatcgc acacacagcg cgctcgtagt aaaaaacttt gatgctttca ccgtcgatgt
    38281 cgtccgccgg gacgtcagcc agacgcgaaa aaccggcagc aattttctgt tcgcggtact
    38341 cgtacagctc cgcattcgtc tccgccatgc ctgacttgat ggcctcacgc agacgggcgg
    38401 gggcgacggt ctgctcaagg cgcatacgtt cccggacgcg cttcgggtca atatcgggaa
    38461 aaaagaacgt gtttttaatc accggctcgt cgcctgccgg ttgcgggata accaccgtac
    38521 cctcaccgga cacgggagcc tcctttcgcg gaataatcag cgtcatcatg actacctctg
    38581 aaaagtcggg cggtggacgc cggtgcagtg tcaggtgatt caccgtcact gaccggcgtg
    38641 ccgccctggc gcggggcgca ttcggttgtt aactggcttt ctttttcggg cgtccacgtt
    38701 ttgccggtgt cacgctccgg gtcttacgcg gagtgcgggt ggccgctttg ggctgcggct
    38761 ccggcttcgg tttcagctcc cgctccagtc gttcaatctc ttttttgacg cctgcctgac
    38821 agtcgagctg tgtcgcacgt tgcaggtgta ccagcgcacc tgcggcatca ccagcgtcac
    38881 gcagaaacag accggtgatt ttgtgcagct ttgcgcgcac ttcatcaggc atgtcagccg
    38941 tggcggtcag ttcgagggtg tccgtcagca ggcggggatc cacagactca ccggcagcgt
    39001 gagcgcgcat ggccgcaagc gccacctcct cggtgaacat gtacggcggg gtgcggcggt
    39061 gtttacccgg catggtcaga ccgtacttca gggcataacg ggcaatctcc agcgcaccgg
    39121 caatatcgcc ggtatccaga cgccacagca tgaccgtcat cagaatgtca tcctgtgcac
    39181 ctttgccctg ctccagcacg ccgttcaccc acggcaacca gaacggcagc agttcgcgtt
    39241 ttttcgcggc cttcagctct tttgaataaa tcgctttcag tgtgcgctgg tctgcggcca
    39301 gcttgaccag catctgctca tagacagttg catgtcgcag cggggcggct tcccgctgcg
    39361 cggtcatcgc tgccgagacc cgcatcatgt ggcgctgtgc gggactcgtc atcggttacg
    39421 ctcccggctc tgcggtcgct ttagccggtg tggagaaatc accgacctta attttttcca
    39481 ccagacaacc ggcggcgtag tcctccacca cgtaatcaat gttcattgac tcgtagttct
    39541 ccacgcggtc gagtttcggg ttttccacaa tcacgcggcg atggctgtca tccatgtagt
    39601 agatggacag gttttccagc ttcgtgatga gcatcgcatc cgccgggaag tacgggacgc
    39661 gtaccgccgg caggttaccg atgcgtttct ggctgatgat gacgtcagcg gccagcatct
    39721 cgctgttatc ctgctccttg ttgacgatag ggaaatactt gtccgccagt agctgacgcc
    39781 ccacaatcac cacaaggtca gggtcttcct gataccaagg ttcaatcagg ttgttggtcg
    39841 catccatcac cagtgcatcg aggcttacat aatcaccgcc cttacccacg cggataacct
    39901 cagaggtggt gcggccttcc tcgtcagtga ccttgctcat cacgcgcgcc ggggcttcat
    39961 tcggtatttc tgcagccagc cgaccgccac atcctgcagc atcggattac tgttgcggtc
    40021 agaggtttcg gcacgcttca cgccgttaaa accggccatg atgaaatcaa gggactggcg
    40081 tttgataatg gcgttacgga tacggagctg gaaatcctga taacgcgccc acaggtcaag
    40141 cgttttgtag cggatataaa aatcgaagtt aacctggtcg cattcgtact tgtttgacgc
    40201 cagcttcgag aagtccttcg gctgacgctc ggtgccaccg gcggtgtcgg tggtgctggc
    40261 gatggagccg gtgacaccga tgccaatttt ttcccctttc atttcactga ccggcacaat
    40321 gttgatgcgg gtcagaaagt cagaggactc ctgcatggtg ttcatcaggg tctgggtgac
    40381 cgacggttca acggtgaatt ttttcgacac atcaccggcg tcgatgccgt tcagctcggc
    40441 aacacgggac aggtaagcat taaatttaaa gcgggtttcc tggcgcatag tttttcctga
    40501 aattaagggt taatcgtgaa ggttttcccg gactgactga cgccggtcag cagttcgtca
    40561 tcagggcgtc accgccacca ccggtggcct tgctgcggcg ctgctgggtc agactttcgg
    40621 tgtggtcgag actgtttttc aggcgggtga atgcctggct ggtttcatcc gccctgtcag
    40681 tcacctcctg cttaagtgca gaaaaggcgg tttccatctc agcgaggcgc tgctcagtgg
    40741 cgctcagttt ttcctgcaca tgttcagcaa cagcggtcac cgcttcatgc acgtcattca
    40801 gacgggcatc atcgctggcc tgtttgcggc caaaaatgga tttcaccttt tcggtcaggg
    40861 cggtgaacac ggtttcaggc aggtcttcaa attccagctc aacaggcgtt gccactgaaa
    40921 tcaggttttc agggcttaat ttgaagcggt tcagggggtt gtgttttgcc gtgcggcaga
    40981 attccaggta ttccgtgccg aggcttgccg ggtcatcggt gacggccaga cccaccagat
    41041 aacatttgcc ggtgttggca aagttcggct gaatttccat tgaggtgtag accttctgcg
    41101 cggccttgtt catcgcgata aggtcatcgg tcggggtgat tttcgcaaac agcgcccatt
    41161 tgcctttcag cgccgaatca tcgtcaatct tttcggcctt cagttcggcc acatcgccat
    41221 aacgcttaaa aataccgtca ggcaggatgc cgcgcagatg ttccaggtta atgcggcaac
    41281 catagacacg cgggtcaaag gtttcggcca tttcctgaat atcctgcgca ctgatgacac
    41341 gcccgtcaca ggtgtcaccc tcaacgccga tacgaaagaa ttttgagact ttttttgcca
    41401 ttgtcaggag tcctgaatag tgattagagg agtcacatgt cggcatcagt ttcccgacga
    41461 tgcgcatcct ccgccatcag tcccggatgg tttatcactg acacaacagc acattagcga
    41521 atcgcggggc gcgactcagt agccttgccg tgtattcatc acggcgaggt attcatgacc
    41581 atcaccacag acaccactct tttacacgac ccgcgtcgtc aggcggcgct gctgtactgg
    41641 caggggtttt ccgtgccgca gattgccgcc atgttgcaga tgaaacgccc gacggtgcag
    41701 agctggaaac agcgcgacgg ctgggacagt gttgccccca tcagccgtgt cgaaatgagt
    41761 ctggaagcgc ggctgaccca gctcatcatc aaaccgcaga aaaccggcgg tgacttcaag
    41821 gaaattgacc tgctcggacg ccagattgaa cgactggcac gggtaaaccg ctacagccag
    41881 accggcaacg aggcagacct taatccgaac atcgctaacc gcaacaaagg cgggcgtcgc
    41941 aaaccgaaaa agaatttttt cagtgacgag gccatcgaaa agctggagca gattttcttt
    42001 gagcagtctt tcgactatca gttgcactgg tatcgtgccg ggcttgagca ccgcatccgc
    42061 gatatcctga aatcccgcca gattggcgcg acgttttatt tttcccgcga ggcgctgctg
    42121 cgcgccctga aaaccggcca taaccagatt tttctgtcgg ccagtaaaac gcaggcgtat
    42181 gtgttccgtg aatacatcat cgcctttgct cgtctggttg acgttgacct gaccggtgac
    42241 ccgattgtcc tgggcaataa cggcgcaaaa ctgatttttc tcggcaccaa ctccaacacc
    42301 gcacagagcc ataacggcga cctgtacgtc gacgagattt tctggatccc gaattttcag
    42361 gtactgcgta aggtggcatc aggtatggcc tcacagagtc acctgcgctc gacctatttc
    42421 tccaccccgt ccacgctggc gcacgacgcc tacccgttct ggtcgggtga actgttcaac
    42481 cggggacgcg ccagcgccgc cgaacgtgtg gaaatcgacg tcagtcataa cgcccttgcc
    42541 ggtggtcttc tctgtgcgga cggccagtgg cggcagattg tcaccattga ggacgccctg
    42601 aaaggcggct gcacattgtt cgacattgag cagctcaaac gcgaaaacag cgccgacgat
    42661 tttaaaaacc tgttcatgtg tgaatttgtt gacgacaagg tatcggtgtt cccgttcgag
    42721 gagctgcaac gctgcatggt cgacacgctg gaagaatggg aagactacgc gccgtttgcc
    42781 gcaaatccgt tcggctcccg cccggtatgg attggttacg acccgtcaca ccgtggcgac
    42841 agcgccggat gcgtggtgct ggcaccgccg gtggtggccg gtggcaaatt cagaatactt
    42901 gagcgtcacc agtggaaagg catggacttt gccacccagg cggagtccat ccgcaaactc
    42961 accgaaaaat acaacgtcga atacatcggt attgatgcca ccggcctcgg tgtcggcgtg
    43021 ttccagctcg tgcgctcgtt ctatcccgcc gcgcgcgata tccgctacac gccggaaatg
    43081 aaaaccgcaa tggtgctcaa ggccaaagac gtcatccgcc gtggctgtct ggaatatgac
    43141 gtcagcgcca ccgacatcac cagctcgttc atggctatcc gcaagaccat gaccagcagc
    43201 ggacgcagtg ccacctatga ggccagccgc agcgaggaag ccagccacgc cgacctcgcc
    43261 tgggcaacca tgcacgccct gttaaatgag ccactcaccg ccggtatcag cactccgctg
    43321 acatccacca ttctggagtt ttactgatga gcaagaaaaa agggaaaaca ccgcgacctg
    43381 cggcaaaaac aatcaccgcc agcgccccga aaatggaagc attcaccttt ggtgagccgg
    43441 tgccggtact cgaccgccgt gacattctgg attacgtcga gtgcatcagt aacggcagat
    43501 ggtatgagcc gccggtcagc tttaccggtc tggcaaaaag cctgcgtgct gccgtgcatc
    43561 acagctcacc gatttacgtc aaacgtaata ttctggcttc aacgtttatc ccgcacccgt
    43621 ggctttccca gcaggatttc agccgttttg tgctggattt tctggtgttc ggtaatgcgt
    43681 ttctggaaaa gcgttacagc accaccggta aggtcatcag actggaaacc tcaccggcca
    43741 aatatacccg tcgtggcgtg gaggaggatg tttactggtg ggtgccgtcc ttcaacgagc
    43801 cgacagcctt cgcgcccggc tccgtgtttc acctgctgga gcctgatatt aatcaggagc
    43861 tgtacggcct gccggaatat ctcagcgccc ttaactctgc atggctgaat gagtcggcca
    43921 cgctgttccg ccgcaagtat tacgaaaacg gcgcacatgc cggatacatc atgtacgtca
    43981 ccgatgccgt gcaggatcgc aacgatatcg aaatgcttcg cgaaaacatg gttaagtcga
    44041 aaggccgcaa taactttaaa aatctgtttc tctatgcccc acaggggaaa gccgacggca
    44101 ttaaaattat cccgctcagt gaagtggcaa cgaaggacga tttttttaat atcaaaaaag
    44161 ccagcgccgc tgacctgctg gacgcgcacc gcatcccctt tcagttgatg gggggcaagc
    44221 cggagaacgt cgggtcgctg ggtgatattg agaaagtggc aaaggtcttt gtccgcaatg
    44281 agcttatccc gttacaggac aggatccgcg agataaacgg ctggctcggt caggaggtca
    44341 tccgctttaa aaactactca ctggacactg acaacggctg aacatcgccg cctgcgggcg
    44401 gcttttttac accccgccat cacgccctca cacgctcatc accgcacaaa aaatcccgca
    44461 gacacaccaa cgcccaacgg gcagactaag cgccgtcacg acgcgctgag acgctggaaa
    44521 ataaaatcag caccaccgcc agcgcgcagt gctttccccg cctcgcccgc ccgcttcatg
    44581 gggcggtttt aatgcagttg catgaacact ccaagagcac gccagcgcta gtgataacta
    44641 acaataaaag cgacttctga cgcatgcaaa tccatgcaca tgatggcatt tttcatgatc
    44701 tatgcggtat gctatccgct tcaattttta acacttgtta agggaactag acatggacat
    44761 tattgattta aaatctcaac tagaaaatga agataatgcc aagaaaagag cagagttgct
    44821 tgctcagcta gaggtgataa agcaaaacgc tgaagagcaa attcgtgctg agcaaaagga
    44881 tgtggatttt gagaccaaag aatttaccgt tgagttatta gttaataagt atcataacgg
    44941 attagaggat gatactaacg aactctttgt tcctgattat caaagggatt ttgtttggag
    45001 tgaaaaaaga caatcaaaat tgatagagtc tttgattttg ggttttccta taccatatat
    45061 atttatagca gatgttctct ccgaagatcc cgatttagat gggcgaattg agattgttga
    45121 tggctcacaa cgagttagga ctatacacgc ttttatccaa aatgaattag ttttacaaga
    45181 cctgaaatct ttagacgtcc ttaatggttt caaattccaa gatttaccat tatcacgtca
    45241 acgtcgtttc atgagaattc ctgtccgcgt tatagaattg agttctaaat gcaatgaaga
    45301 aacccgtaga gatctttttg agcgtattaa ctctggcagc gatatattga aagatatgga
    45361 agtccgtaaa ggatcagaac ttggttcaac aaatttatat acacaggtta ttaagccatg
    45421 ctcaacaata cctaaattta aagaattagc acctttatct cagactaaag aaaaacgaga
    45481 tgaacggtta gaatttacat taagattctt tgcctacgta gataagtaca agaactttga
    45541 tcattccgta cgggattttc taaatgacta tatgtcagaa aatcgagata tagacgaaac
    45601 taaacaaaac tcaatgaaac aagagtttgc taatgttcta tcttttgttg aaaactattt
    45661 cccagcagga ttcaagaaaa ctattactgc aaaatctaca cctcgagtta gattcgaatc
    45721 tctcgctgtt ggcgcagcac ttgcattacg tgaagtccct gaccttgttc ccaagaactt
    45781 agattggcta agctcagaag aattcaaacg attgaccact tccgacggag ctaatagtcg
    45841 cgtgaaggta attgaacgaa ttgaatatgt tcgggataaa ttattagagg attaacatgg
    45901 atcgttttcg tgaggatttt gacgaaaggt caggagagat cttggcctat ttggatcttt
    45961 tgaaatttat tgagtatgcc ggtgcagaac tgatatcagc tgatgataaa gaacataagt
    46021 tctccattac tgctcaatca cgaaaaactc taaagggagc agtatatata ctgttatata
    46081 acctcattga atcaacaatg agagaagcta tctgccttat tcatgagact atttatgata
    46141 gaaacgtaga atttgacaaa ctcaggaaaa atctcaggtc agaaatatta aaaagattaa
    46201 aaaatgaatc tgtaaatgtt gaaagtttag ttaatcacct taccaaaggt atatcttgcg
    46261 gtatatctta tggcacgttc aacaaaaaga aaatattctc aggaaatatt gatagagagg
    46321 aaatcaaaga aaaatcgaaa atatatggtt tttctacaca ttccgattac actcacacta
    46381 aacatggtga aaaattagcc actgttaaac aacatcgtaa tgatttatct catggcaatg
    46441 tatcatttgc tgagattgga aaaaatgttt catatcagga cttagagaat atttcattag
    46501 aagtaatcgc ctatcttgat gctatcgtga ataatataga gcactatata aataataatg
    46561 aatatttaga acaatagtta taaggcctgt aaagcaggcc attttatcat tctaaatggt
    46621 tcttgattga ttgcccaatt gcttttccta gttccacagg aacagcatta cctatcattt
    46681 tcccaatact tcttataaca aacttgtttt tgtcttctac aaaaatataa tccatcggga
    46741 aagtttgcaa taaagcagcc tctctaagag aaatcgctct atgctgttct ggatgtccaa
    46801 aacgcccatt cccgaaacca taacaaagag ttgtaattgt tggacttggc ttatcccaag
    46861 acatgcgccc atatacacta gcataccctt taccgctgga tttcatatga caagcggcaa
    46921 ccaactcttt cggccagtcc ttccatgtac caccaggaac agagtgtata attctttttt
    46981 tattaatcgg atttaattta ctggctctat gtaatatatc atttggatct gtttggccat
    47041 cttctaatgg aggaagattt cctattgcat ctctaactgt tttcaaaata acatcgtcac
    47101 gttttactaa ctcaatcgcc cccaattttg aggccaacaa tacatgacgg gaacggtttt
    47161 gtgggattcc atattcgaca caatcaatct tagacgccca aacataatag cccaacttta
    47221 acaatgaatt ataaaaatca ttataaactt tatgtttagt tacatcagga acgttctcca
    47281 ttgttacgat ttctggagat acttcgcgaa ttaaacgttc gaactcataa agaagaggcc
    47341 attttttatc atcagcctta tcttttcctt gagtatattt tgaaaaaggt tggcaaggtg
    47401 cgcatcctgc caatactttt acagacgcat caccataaag cctcagcagt tcctcttttg
    47461 ttactttagc gatatcttgc tcaataaact tagatttatt gtttctttca tagggaaacc
    47521 tacattcccc ctcaaggtca atgcctgcaa cgacatcaag ccccgcaagc tgcaaaccat
    47581 gagttaatcc gccagcacca caaaaaaggt ctatcacctt tacagtcatc actcactcca
    47641 aatctatacg atactcagta tacatccctt tctacaaaat ctcgaggcct aacttataaa
    47701 ctcatattta ttgagttaac gcttcactga gtttgactct aatactgttg agaatcccga
    47761 ccactcatca gcgagcgggt aagtgaattt tttcccgtca taatttacgg ttgccccacg
    47821 cgccagcacc tcaagctccc atcgctgcgg cctgataccg ttctgagcaa ggtcaacgcg
    47881 gatacgggta atttgcattc gttccgaccg ggtcagtctg gccgatggcg ctatttcatg
    47941 cggttttaac gggcttccgt ttctttgctg acggtttggt cttctcagtc cgtgttttaa
    48001 tgcacctctg agcgccctca caacctccgg gtcattccat tcgataacac cgtcatctac
    48061 cagattaagc actgctgcgg cgtgctcaga aggtgtggga gccggtaacg aagtatcacc
    48121 accagtgagc tttccacagt tattgacagg actccgaggc gcggcgatgc cgctttttaa
    48181 agtcaaaggc tcaacgaccg gcactttcgg aacaatgcgc cagtccgtcg ttctggtgat
    48241 atgaatatga cgcgcgccga gatgcggcgc gtaaatgccg accactctct cgacttcttc
    48301 ctcgtactcg ttaacttcat ccgacggact acgggcgacc ctgacagtct gacaatcgcg
    48361 cgggacattt gccccaccct gcgcgctgat atacagcgca aaatcaccac tgtctgcggc
    48421 ggcgcgtgca gcctcgacgc gctcgtcaaa ctcatcagca atgctgacgc cgcgaggcaa
    48481 tttgcgtagt tcacggtaag cccccattgt cggcaggcca accgttttaa attgcggaat
    48541 gcgccacgtt gacgcccatg cggtaacagc cgcggcagta tctttcagcg gtctgccgct
    48601 atcgttatcg agctgcccat ccagtgcata gccgtcgata ttttttgaga tgtatttcgc
    48661 gatatacccc gcagcaccgc cccgattaag atgttttgcc tgaaaacggt ttcgcgcagc
    48721 tcctctttcg tcgccatcct ctttgagcgc gtagcgacgc atgatttcga taatctggtt
    48781 acgctggcgt ggattacaaa aaagcatcat atgccagtgc ggcgttccgt cgtggtgtgg
    48841 ctcgacgact cgcaaaccgt agacctgtaa atcattatcc ttgaatgccg tgcgcatcag
    48901 gctccagata cggcagagat aacgctgcgc atcctttgga ttaaatgcct catcgttcca
    48961 gccgtgattt agctggacgg ttttactttc gccttttcca acctgacgtg tcgggtgata
    49021 ctttgacggc gcggtcagcg tgataaacat ccccacatca ccttctgcgg cggcgtaacg
    49081 ctcaataccg gcaatggtgt tcatcagctc catccggcga atttcaggat tagaaatact
    49141 gcccatcacc ttactgataa ggtcgatgcg ctcgcctgtt tccctgtttt cgagatcaca
    49201 cgatttaaga aattccagat ttgcctggcg gcgtgcacgc acatcacgaa tggcatgttt
    49261 actggcataa ggagaacggt ctttattgac ctccccgaca gcaatcagta acgcctcatg
    49321 ccagcgcata cgctggcctt taagctgact aatccaccac tcatcgttaa acagacgggc
    49381 aatggcagaa tatgcctgcc tcgtggtcat ctgtccttta cggtattttt tccagtagag
    49441 aggggaaata ttgaaagcac gtgcagcgcc agcaacatga ccatagaggt gagcctgcgc
    49501 ctcatccgta aacagcgatt ctttttcgcc atgcgcatcc acccaggcat cgctgagttc
    49561 ctcatacatc atgaaaagct gcgatgagat acgggcggca aactttttca gctccttgtc
    49621 attcattccc ggcaggcgcg catagtggtc acgctcagcc agaaacagca tcgacgcgtc
    49681 ggtgttcatt tcatggcgct gattcacacg ctcaatgcgc ggccataaac gacgctgaaa
    49741 agtggatgtg aggaaataaa acccgtgcac cgggctttta ttgcgccgga tgtagtcata
    49801 gcgtgacgta aacagcgagc gcaaaaagta aggcaggcgg ttaatcgtgg ataaaacacc
    49861 ttgcacctga cgcatctcgt cacgtgtaag gggtctttcg cgcccgacag cctcgcgtgg
    49921 cgcgttccat gcataagcac cggtaaacgc cttaccggtg cctgcagcaa atgctgacgg
    49981 agggacaaaa cgcccggagg ctttaacggc catatgagcc aaaagcctct gaacaacgct
    50041 tgctgagttg ctcaacctgc gcgtttaaat cagcaaaaga ctttgcgctt ccggtcagaa
    50101 tatcgtgatg catcaggccg gaaacgagct ggcttaattt cgggtaataa ccaaccaccg
    50161 ccagccattc ctgaccggcg tttttaccgc tttccgctct ctttttctcg tggagaataa
    50221 actggaagct gtcactggta acgacataac gttcgccaat ttcaatacga atactcatgc
    50281 cgttctccgg taatgtttgt tttttgcttc aaagactgac tgacaggaaa cacaacgcgt
    50341 ggctgacgga taagccgcac gacgggcagc aggaattggc gcgtcacact cttcgcaaac
    50401 cagcgcagaa gcaccgcaat gttttaccct tgccgcgtta atctggcgct ccagtaattc
    50461 agcctgttgt tcctgaataa aatctatgtt gtccggcatt atcagctcct tttatcgtta
    50521 agtttcctgg atacatcagc gcaataactg gcaagttctg tcgttaattt tgtcagttca
    50581 tccactgagg aaatttgctt gtggaataca gcgcgtttaa caagtaaatt gaccacatca
    50641 gacaggaggt ttaattcatt ctgataaatc gcgataacag attcagtgat gtcgcgtttt
    50701 tctttatcaa gacaaagttg aataagagac aagtcgccat ttttcataac ggcgattttt
    50761 aaggcgttat tcagtaatac aactgaatga gaacaggaca tcaaagcacc tccccgcgag
    50821 acaatccgat attgtgaaat ttttccgact cctgactgag cagctcgact atctccacgc
    50881 gggataactc cgcctttgtg atatggcgaa tcatggcgtc aagatgagaa gaaaagcgcg
    50941 tcgcagcgtc ggcctgtgct tcggttctgg cctgttgcag cagtaatgcg tatttaccgc
    51001 actgattttc agaaactgta tgcatgactt tctccaggca aaaagaagcc ccgcacaatt
    51061 aagtgcgtta aaaactctgg ttaattattt aatgcaaata ttgctctggt tttaccgacg
    51121 tcagaattgt cggtgcatac tcaaacagac tgaatagttc acgtaatgca cggaataaag
    51181 catcacgcca gtaacatgac tcttcattaa ttcgccagta tggctgattg aattcttttt
    51241 cagtcaatcc ggcatgcata aataaagtac gacgctgact gactgttaaa aaactaatat
    51301 atgcatactc acttgcgcca acctgacggc gttttgagaa tgccccacgc aattcatcaa
    51361 ttgcacaaac cagccgttca cgttcgacgt cgttcatttc ttcaaaacgc atcgttgcgt
    51421 gacgttgttt taactgcgca tgaaagcaaa ctgttagccg ttcgcgctcc atcatctgat
    51481 tataataatc acatgtatcc tgccaacgag ggacggcaag atgcttacca attatccggc
    51541 gcatagctgc tggctgtttt tcgacgagat tgagcgtcat cactgtcatt tccagaccct
    51601 ccggcttttc agaaaggtca gagccttttt taacggactc tgttttttgg tgcggataat
    51661 gattcccttg cgacccttcc cgtgggtgat ggtgaagtca atcgccctag ggctttcgtt
    51721 acgcaataac tgagcaatac aacgcggctc attcataatc acaaccccat ccacaaaagc
    51781 catgcatcac gctgttcaac cggtcggtta taaaacgcct cacgtacagc gcgattaaac
    51841 tcaggaatga aaacccattt ttcaccggca cgagccttcg gtttgcaagg atcacgcaat
    51901 tcaataattg gtaatttatt tgccttcacc atttcactga cggctgtctt tggcttccct
    51961 aataaatcag caaatttatc cacatgaacc gcatcaagcg gatactgaat cacataattt
    52021 tcagcgtcca tatatggtac cctcatagga tccagccctt tctaaaccac tcaaaaccgt
    52081 ttagacgctg gtttattctc aaatcaatgg aacctatata ggttccagtt ttgagggaat
    52141 ttagtcccta tataggcacc atgtcaaatg aaattaagcg aaaagattaa ggccttgcgt
    52201 gaggctgaag ggctaagcca atcaaaattc tgtgaaatca tagagttacc gctaagcaca
    52261 cttaaaaaat atgaaggagg aaactttgaa cccggtggca cagctttgct aaaaatcact
    52321 atgcatccca cattccaaaa atatgctcta tggcttatga cagataaaac cgcgccggac
    52381 gcaggacaaa tcgcaccggc tctcgcgcac attgggccag agtcaacaga gtccaaccac
    52441 tccgcgaaaa ggattggcta actctatata aagattacat tttcaccatt tgctaccaag
    52501 atggtgaata cagcgccgga gggctttctt atggcaatta agaagctcga tgatggtcgc
    52561 tatgaagtgg acattagacc tcgcggtcgc gacggaaaac gcatccgcag gaaattcgaa
    52621 agaaaagctg aagcactagc atttgagcga tacacaatcg ccaatgccag tcagaaagaa
    52681 tggggaggcc agcgagcaga ccgccggact ttgagtgagt tgctggacat ctggtggaaa
    52741 tatcacgggc aaaaccacga gcatggaaca aaagagttta atcatctact caaaaccatc
    52801 agcggcatag gtgatatacc agtgagcagg atgagcaaaa gggctttgat ggattatcgt
    52861 tccatgcgac tacgtgatgg tatcagtgcc gcaacgataa accgtgacat gtacagatta
    52921 tccggcatgt tcacaaaatt aattcaattg gatgaatttt ccgggcaaca cccaattcac
    52981 ggactgccgc cactggcgga ggccaaccct gaaatgacgt tcctggaaaa agcagaaatc
    53041 gaaaaactgt taaatgtttt ggctggtgat gacttacttg tcgcgctttt atgtctgagc
    53101 actggaggaa gatggacgaa agttgccacg ctaaaaccag cacagattac aaattgcagg
    53161 gttaccttcc tgaaaaccaa aaacggtaaa aagcgaaccg tgccgatttc tgaggaactg
    53221 gagaaaaaag ttaaagagga ggccagcgcc aaattattca aagttgatta tgagaaattt
    53281 tgcgggattt tacgcagagt gaaacctgat ataccaccca atcaggcaac ccacatcctg
    53341 cggcatacat tcgcaagcca tttcatgatg aatgggggca atataatcgc actgcaacag
    53401 attctgggac atgcgagcat tcagcagacg atggcctatg cgcaccttgc gcctgactat
    53461 ctgcaaaatg ccgtcgctct gaatccacta aaaggcggag tgacgttata aatttccccc
    53521 tctgagtgtc cacatagtgt ccacactcaa gaaattttga aaccgctcca aacccttaca
    53581 gctttcgata agtaactgtt tttacttttg attgcatgta agtatttgat aaaaaaaacc
    53641 ccacatcatg tgggggaaga cagggatggt gtctatggca aggaaaacag ggtttactac
    53701 tgggaacgtg agttgctact actcaatagc ttcaacgatg aacttttttg ccattgcgtc
    53761 acgtcacgca actgctccat tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc
    53821 ggcgttaaca ggcgatacat ttggttgcgg acttttgcca tctcaacctg acgagcaatt
    53881 tgctcattcg ccattttttc tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg
    53941 gtgacaaggc gatgcattgt ctccagttcg ctaacattaa caggaggctg ttcgtgccgg
    54001 gcctgttgca taagatctcg catctgctga cgctgatgtt cggttaaact tatgccgtcg
    54061 aacatatggc tctgcgtact gcgctgcgta agttcttcac ccggatgcca gttatcgcct
    54121 gaaccgactt cagcagcgtg gcttaatgaa ctgactgcca gcgttgaggc catgacggca
    54181 gcggtaacta tgcgcatcat ttgctcccaa aatctttctg tcgcgattca acgatagaga
    54241 gtttacgatt caggctgcaa acatgcgtca gggggtgtaa aacaacgtaa agtcatggat
    54301 tagcgacgtc tgatgacgta atttctgcct cggaggtatt taaacaatga ataaaatcct
    54361 gttagttgat gatgaccgag agctgacttc cctattaaag gagctgctcg agatggaagg
    54421 cttcaacgtg attgttgccc acgatgggga acaggcgctt gatcttctgg acgacagcat
    54481 tgatttactt ttgcttgacg taatgatgcc gaagaaaaat ggtatcgaca cattaaaagc
    54541 acttcgccag acacaccaga cgcctgtcat tatgttgacg gcgcgcggca gtgaacttga
    54601 tcgcgttctc ggccttgagc tgggcgcaga tgactatctc ccgaaaccgt ttaatgatcg
    54661 tgagctggtg gcacgtattc gcgcgatcct gcgccgttcg cactggagcg agcaacagca
    54721 aaacaacgac aacggttcac cgacactgga agttgatgcc ttagtgctga atccaggccg
    54781 tcaggaagcc agcttcgacg ggcaaacgct ggagttaacc ggtactgagt ttaccctgct
    54841 ctatttgctg gcacagcatc tgggtcaggt ggtttcccgt gaacatttaa gccaggaagt
    54901 gttgggcaaa cgcctgacgc ctttcgaccg cgctattgat atgcacattt ccaacctgcg
    54961 tcgtaaactg ccggatcgta aagatggtca cccgtggttt aaaaccttgc gtggtcgcgg
    55021 ctatctgatg gtttctgctt catgataggc agcttaaccg cgcgcatctt cgccatcttc
    55081 tggctgacgc tggcgctggt gttgatgttg gttttgatgt tacccaagct cgattcacgc
    55141 cagatgaccg agcttctgga tagcgaacag cgtcaggggc tgatgattga gcagcatgtc
    55201 gaagcggagc tggcgaacga tccgcccaac gatttaatgt ggtggcggcg tctgttccgg
    55261 gcgattgata agtgggcacc gccaggacag cgtttgttat tggtgaccac cgaaggccgc
    55321 gtgatcggcg ctgaacgcag cgaaatgcag atcattcgta actttattgg tcaggccgat
    55381 aacgccgatc atccgcagaa gaaaaagtat ggccgcgtgg aactggtcgg tccgttctcc
    55441 gtgcgtgatg gcgaagataa ttaccaactt tatctgattc gtccggccag cagttctcaa
    55501 tccgatttca ttaacttact gtttgaccgc ccgctattac tgctgattgt caccatgttg
    55561 gtcagtacgc cgctgctgtt gtggttggcc tggagtctgg caaaaccggc gcgtaagctg
    55621 aaaaacgctg ccgatgaagt tgcccaggga aacttacgcc agcacccgga actggaagcg
    55681 gggccacagg aattccttgc cgcaggtgcc agttttaacc agatggtcac cgcgctggag
    55741 cgcatgatga cctctcagca gcgtctgctt tctgatatct ctcacgagct gcgcaccccg
    55801 ctgacgcgtc tgcaactggg tacggcgtta ctgcgccgtc gtagcggtga aagcaaggaa
    55861 ctcgagcgta ttgaaaccga agcgcaacgt ctggacagca tgatcaacga tctgttggtg
    55921 atgtcacgta atcagcaaaa aaacgcgctg gttagcgaaa ccatcaaagc caaccagttg
    55981 tggagtgaag tgctggataa cgcggcgttc gaagccgagc aaatgggcaa gtcgttgaca
    56041 gttaacttcc cgcctgggcc gtggccgctg tacggcaatc cgaacgccct ggaaagtgcg
    56101 ctggaaaaca ttgttcgtaa tgctctgcgt tattcccata cgaagattga agtgggcttt
    56161 gcggtagata aagacggtat caccattacg gtggacgacg atggtcctgg cgttagcccg
    56221 gaagatcgcg aacagatttt ccgtccgttc tatcggaccg atgaagcacg cgatcgtgaa
    56281 tctggcggta caggattggg gctggcgatt gttgaaaccg ccattcagca gcatcgtggc
    56341 tgggtgaagg cagaagacag cccgctgggc ggtttacggc tggtgatttg gttgccgctg
    56401 tataagcgga gttaaactcc gcatttgtag gcaggataag gcgtttacgc cgcatccggc
    56461 atttgagcag gatgcctgat gcgacgctga tagcgtctta tcaggcctac actccggggg
    56521 ttattttccc cacaaccggc gggaaaaatc ttcgatcttg ccgctcagtc ggcgcttttg
    56581 catcgtccgc gtccagcttt tcgataaccc ggcggcggag agtaaacggt gatactgatc
    56641 gtcatcaaac ggcatatgcc atgcgatggc ggcagcctcc tgcacggtca catcgctgac
    56701 acgggaaacc aactccagcg gcgcgtccgc agacactttc cctggcgcta tcacgctata
    56761 caaccacccc accttacctg tgttttgcat caactgcgct atatcgctga tatcaaaatg
    56821 gtaattgagt ttatagcaag gcgagcgcgg ctggctgacc tgaattaatg cctctcccca
    56881 gcggaaaata tcgcccatat gaacattact ttccgtgaga ccgtcggttg agagattttc
    56941 gccaaacgca ggcgcaacaa acaactccgc ctgttccgga aattcccgca cccagtagag
    57001 atagtgctca cgaggataat gacacagcgc tctgtctggc ccgccgtgaa cctttttctc
    57061 cgcctgctcg tcaccttcca gccccagctc cgtcagcatc aactcaccat caacctggat
    57121 tttggcaatt gcgctgggtt tgccttcggg ataagcctga atcttgcctg tgtatacatc
    57181 aaccggatat cgcatctgtt ccctctccac gagaaatttc cccattagac catatttatt
    57241 ttatgtcagg cgtgctgttt tcgcggtaag tatccaggca ggaactcaat agcaaaccgg
    57301 acgaacgtta gttcatccgg ctgtggggat taaggaagca ggtttatttc accgtaccta
    57361 aaatttcgat tttcgctgct ctggctttga ctttacgtga ccagatagag gtaagaattg
    57421 gcaccagaat cgaagtcaca atgaccgccg tcgctaccag cgaagttgct gccggagcca
    57481 tcggtttaaa cgcaggcacc atttctgcaa tcagcacagg tgtcgctacc gcggcccctg
    57541 cggaactgga agcggcaatt ccggcagtgc catcgccacc gccaatcaat ttatcggcga
    57601 taatcaacgg aataccggtc acgataatta ctgccacacc caacaggatc cccagcaaac
    57661 cagtctgggc aatcacagtc aaatcaatgg tattgcccag cgcgaaggca aagaatggaa
    57721 tcagcgtttg cactgctttg ctgaaaaatt ctcgtaattc agggtcaaga ttccccaggg
    57781 caaagcccac caggaacggt aatactgcgc cgacgaaaac atgcggttca aacgaggcaa
    57841 tcccggcagt gcccagaata atcatcgtca tgagcggccc ggactccaac gacatcaaca
    57901 caaatgcccc agcttcttct ttcgtaccgt actgctgcat gatggaagcg taaagtccgc
    57961 cgttggtcat atccatcgcc gccaccagcg ccagcgttga aagtccggca aagaatccaa
    58021 cttcaacacc atgttccgga ataatgcgcg aggcaattgc cgcaaccacc cacgcgacgg
    58081 caattttagt taccaccagc gtaccggatt tacgcagtac cgttcccgtc gcgcttaatt
    58141 ttattgacgc ccccatgcaa aaaaaccaca ccgccagaat gggcaccgta ccggtaatca
    58201 tcccgttggt gaatgatcca aaatatttcc ccgcccccgg cgagaaggtg tggcacagtg
    58261 cgccaaggaa tagcgggacg agcatcatcc cccccgggat tttctcaatc gagcgtttta
    58321 tctgcatttc catcacctgt cggcatattt ttaagggctg atgcgtaatt cctcaatgga
    58381 gcatcagtgt tgatggaaac gaagatagac attcatttaa tcaataaaag tgatcaaaac
    58441 cacatcaatt gaaacgctgt tttattttta tcggatcatt gtttttattt tgaaattgat
    58501 cacaaaaaaa ccaccgccgt tggcgatggt tcatttttta agctgatatg cggcctatac
    58561 gcctcattgc agcaggcggc aaatgattat tttttcgccg caaaacgtgc cgctgcttcg
    58621 tcccagttca ccacgttcca gaactcttta atgtagtccg gacggcggtt ctggaatttc
    58681 aggtagtaag catgttccca cacatccagg cccagaatcg ggaagccgga agcgccagaa
    58741 atagcttcac ccatcagcgg agaatcctgg ttagcagtag aaaccaccgc cagtttatcg
    58801 cctttcagca ccagccatgc ccagccggaa ccaaagcggg aagctgccgc tttttcaaat
    58861 tctgctttga agttatcaac ggagccgaag tcacgttcga tagccgcttt caggtcaccc
    58921 tgcagggtgg tgcctttttt cagacctttc cagaacaggc tgtggttagc gtgaccgcca
    58981 gcgttgttgc gcagtacggt tttcttgtct gctggcagct ggtccagttt ggtgatcagc
    59041 tcttcaaccg gcaggttggc aaattctggc aggctttcca gcgccgcgtt ggcgttgttt
    59101 acgtaggtct gatggtgttt ggtgtggtgg atttccatgg tctgcttatc gaagtgcggt
    59161 tccagggcat cgtaagcata cggcagggat ggcagggtat agctcatatt catctccagt
    59221 attgtcgggc ggccgattgt taatgccgcg taagcagttg gttcattata gttaattaaa
    59281 tgatattgaa aatgattatc aatgccgtac ttttcgtaag ggtatggttt tgcaggaaaa
    59341 tgcccgagat gtgaagcaaa tcacccactt aatgccgtga ttgccagtaa atcgacaacg
    59401 gcggcaacag gcgaaaggtt aatcgacagc acgattttta cactcatctc gtcggagatg
    59461 tgaagcgacg aaaaatgatg aggataagaa gatgagtaac gcgattacga tggggatatt
    59521 ttggcatttg atcggcgcgg ccagtgcagc ctgtttttac gctccgttca aaaaagtaaa
    59581 aaaatggtca tgggaaacca tgtggtcagt cggtgggatt gtttcgtgga ttattctgcc
    59641 gtggaccatc agcgccctgt tactaccgga tttctgggcg tattacagct cgtttagtct
    59701 ctctacgctg ctgcctgttt ttctgttcgg cgctatgtgg gggatcggta atatcaacta
    59761 tggcctgacc atgcgttatc tcggcatgtc gatgggaatt ggcatcgcca ttggcattac
    59821 gttgattgtc ggtacgctga tgacgccaat tatcaacggc aatttcgatg tgctgattaa
    59881 caccgaaggc ggacgcatga cgttgctcgg cgttctggtg gcgctgattg gcgtagggat
    59941 tgtaactcga gccgggcagt tgaaagagcg caagatgggc attaaagccg aagagttcaa
    60001 tctgaaaaaa gggctggtgc tggcggtgat gtgcggcatt ttctctgccg ggatgtcctt
    60061 tgcgatgaac gccgcaaaac cgatgcatga agccgctgcc gcacttggcg tcgatccact
    60121 gtatgtcgct ctgccaagct atgttgtcat catgggcggc ggcgcgatca ttaacctcgg
    60181 tttctgtttt attcgtctgg caaaagtgaa ggatttgtcg ctaaaagccg acttctcgct
    60241 ggcaaaaccg ctgatcattc acaatgtgtt actctcggca ctgggcgggt tgatgtggta
    60301 tctgcaattc tttttctatg cctggggcca cgcccgcatt ccggcgcagt atgactacat
    60361 cagttggatg ctgcatatga gtttctatgt attgtgcggc ggtatcgtcg ggctggtgct
    60421 gaaagagtgg aacaatacag gacgccgtcc ggtaacggtg ttgagcctcg gttgtgtggt
    60481 gattattgtc gccgctaaca tcgtcggcat cggcatggcg aattaatctt tctgcgaatt
    60541 gagatgacgc cactggctgg gcgtcatccc ggtttcccgg gtaaacacca ccgaaaaata
    60601 gttactatct tcaaagccac attcggtcga aatatcactg attaacaggc ggctatgctg
    60661 gagaagatat tgcgcatgac acactctgac ctgtcgcaga tattgattga tggtcattcc
    60721 agtctgctgg cgaaattgct gacgcaaaac gcgctcactg cacgatgcct catcacaaaa
    60781 tttatccagc gcaaagggac ttttcaggct agccgccaac cgggtaatca gcttatccag
    60841 caacgtttcg ctggatgttg gcggcaacga atcactggtg taacgatggc gattcagcaa
    60901 catcaccaac tgcccgaaca gcaactcagc catttcgtta gcaaacgaca catgctgact
    60961 actttcatgc tcaagctgac cgataacctg ccgcgcctgc gccatcccca cgctacctaa
    61021 gcgccagtgt ggttgccctg cgctggcgct aaatcccgga atcgctccct gccagtcaag
    61081 attcagcttc agccgctccg ggcaataaat aatattctgc aaaaccagat cgttaacgga
    61141 agcgtaggag tgtttatcgt cagcatgaat ataaaagaga tcgccacggg taatgcgata
    61201 aggacgatcg ttgagtacat gcagaccatt accgcgccag acaatcacca gctcacaaaa
    61261 atcatgtgta tgttcggcaa agacatcttg cggataacgg tcagccacag cgactgcctg
    61321 ctggtcgctg gcaaaaaaat catctttgag aagttttaac tgatgcgcca ccgtggctac
    61381 ctcggccaga gaacgaagtt gattattcgc aatatggcgt acaaatacgt tgagaagatt
    61441 cgcgttattg cagaaagcca tcccgtccct ggcgaatatc acgcggtgac cagttaaact
    61501 ctcggcgaaa aagcgtcgaa aagtggttac tgtcgctgaa tccacagcga taggcgatgt
    61561 cagtaacgct ggcctcgctg tggcgtagca gatgtcgggc tttcatcagt cgcaggcggt
    61621 tcaggtatcg ctgaggcgtc agtcccgttt gctgcttaag ctgccgatgt agcgtacgca
    61681 gtgaaagaga aaattgatcc gccacggcat cccaattcac ctcatcggca aaatggtcct
    61741 ccagccaggc cagaagcaag ttgagacgtg atgcgctgtt ttccaggttc tcctgcaaac
    61801 tgcttttacg cagcaagagc agtaattgca taaacaagat ctcgcgactg gcggtcgagg
    61861 gtaaatcatt ttccccttcc tgctgttcca tctgtgcaac cagctgtcgc acctgctgca
    61921 atacgctgtg gttaacgcgc cagtgagacg gatactgccc atccagctct tgtggcagca
    61981 actgattcag cccggcgaga aactgaaatc gatccggcga gcgatacagc acattggtca
    62041 gacacagatt atcggtatgt tcatacagat gccgatcatg agcgcgtacg aaacagaccg
    62101 tgccaccggt gatggtatag ggctgcccat taaacacatg aatacccgtg ccatgttcga
    62161 caatcacaat ttcatgaaaa tcatgatgat gttcaggaaa atccgcctgc gggagccggg
    62221 gttctatcgc cacggacgcg ttaccagacg gaaaaaaatc cacactatgt aatacggtca
    62281 tactggcctc ctgatgtcgt caacacggcg aaatagtaat cacgaggtca ggttcttacc
    62341 ttaaattttc gacggaaaac cacgtaaaaa acgtcgattt ttcaagatac agcgtgaatt
    62401 ttcaggaaat gcggtgagca tcacatcacc acaatgaagc aaattgtgaa catcatcacg
    62461 ttcatctttc cctggttgcc aatggcccat tttcctgtca gtaacgagaa ggtcacgaat
    62521 tcaggcgctt tttagactgg tcgtaatgaa attcagcagg atcacattat gacctttcgc
    62581 aattgtgtcg ccgtcgatct cggcgcatcc agtgggcgcg tgatgctggc gcgttacgag
    62641 cgtgaatgcc gcagcctgac gctgcgcgaa atccatcgtt ttaacaatgg cctgcatagc
    62701 cagaacggtt atgtcacctg gaatgtggat agcctggaaa gtgccattcg ccttggatta
    62761 aacaaggtgt gcgaggaagg gattcgtatc gatagcattg ggattgatac ctggggcgtg
    62821 gactttgtgc tgctcgacca acagggtcag cgtgtgggcc tgcccgttgc ttatcgcgat
    62881 agccgctcca atggcctaat ggcgcaggca cagcaacaac tcggcaaacg cgatatttat
    62941 caacgtagcg gcatccagtt tctgcccttc aatacgcttt atcaactgcg tgcgctgacg
    63001 gagcaacaac ctgaacttat tccacacatt gctcacgctc tgctgatgcc ggattacttc
    63061 agctatcgcc tgaccggcaa gatgaactgg gagtacacca acgccacgac cacgcaactg
    63121 gtcaatatca atagcgacga ctgggacgag tcgctactgg cgtggagcgg ggctaacaaa
    63181 gcctggtttg gtcgcccgac gcatccgggt aatgtcattg ggcactggat ttgcccgcag
    63241 ggtaatgaga ttccggtggt cgccgttgcc agccatgata ccgccagcgc ggttatcgcc
    63301 tcgccgttaa acggttcacg cgctgcttat ctctcttctg gcacctggtc attgatgggc
    63361 ttcgaaagcc agacgccatt taccaatgac acggcgctgg cagccaacat aaccaatgaa
    63421 ggcggggcgg aaggtcgcta tcgggtgctg aaaaatatta tgggcttatg gctgcttcag
    63481 cgagtgcttc aggagcggca aatcaacgat ctcccggcgc ttatcgccgc gacacaggca
    63541 cttccggcct gccgcttcat catcaatccc aatgacgatc gctttattaa tcctgaggcg
    63601 atgtgcagcg aaattcaggc tgcgtgtcgg gaaatggcgc aaccgctccc ggaaagtgat
    63661 gctgaactgg cgcgctgcat tttcgacagt ctggcgctgc tgtatgccga tgtgttgcat
    63721 gagctggcgc agctacgcgg tgaagatttc tcgcaactgc atattgtcgg cggcggctgc
    63781 cagaacacgc tgctcaacca gctatgtgcc gatgcctgcg gtattcgggt gatcgccggg
    63841 cctgttgaag cctcgacgct cggcaatatc ggcatccagt taatgacgct ggatgaactc
    63901 aacaatgtgg atgatttccg tcaggtcgtc agcaccaccg cgaatctgac cacctttacc
    63961 cctaatcctg acagtgaaat tgcccactat gtggcgcagc ttcactcgac acgacagaca
    64021 aaggagcttt gcgcatgacc actcaactgg aacaggcctg ggagctggcg aaacagcgtt
    64081 tcgcggcggt ggggattgat gtcgaggagg cgctgcgcca acttgatcgt ttacccgttt
    64141 caatgcactg ctggcagggc gatgatgttt ccggttttga aaacccggaa ggttcgctga
    64201 ccggtgggat tcaggctact ggtaattatc cgggcaaagc gcgtaatgcc agtgagctac
    64261 gagccgatct ggaacaggct atgcggctga ttccggggcc gaaacggctt aatttacatg
    64321 ccatctatct ggaatcagac acgccagtct cgcgcgacca gatcaaacct gagcacttca
    64381 aaaactgggt tgaatgggcg aaagccaatc agctcggtct ggattttaac ccctcctgtt
    64441 tctcgcatcc gctaagcgcc gatggcttta cgctctcgca tcccgacgac agcattcgcc
    64501 agttctggat tgatcactgc aaagccagcc gtcgcgtttc ggcctatttt ggcgagcaac
    64561 tcggcacacc atcggtgatg aacatctgga tcccggatgg tatgaaagat atcaccgttg
    64621 accgtctcgc cccgcgtcag cgtctgctgg cagcactgga tgaggtgatc agcgagaagc
    64681 tggatccggc gcaccatatc gacgccgttg agagcaaatt gtttggcatt ggcgcagaga
    64741 gctacacggt tggctccaat gagttttaca tggggtatgc caccagccgc cagactgcgc
    64801 tgtgcctgga cgccgggcat ttccacccga ctgaagtgat ttccgacaag atttccgccg
    64861 ccatgctgta tgtaccgcag ttgctgctgc acgtcagccg tccggttcgc tgggacagcg
    64921 atcacgtggt gctgctggat gatgaaaccc aggcgattgc cagtgagatt gttcgtcacg
    64981 atctgttcga ccgggtgcat atcggcctcg acttctttga tgcctctatc aaccgcattg
    65041 ccgcgtgggt cattggtaca cgcaatatga aaaaagccct gctgcgtgcg ttgctggaac
    65101 ctaccgctga gctgcgcaag ctggaagcgg cgggcgatta cactgcgcgt ctggcgctac
    65161 tggaagaaca gaaatcgttg ccgtggcagg cggtctggga aatgtattgc caacgtcacg
    65221 atacgccagc aggtagcgaa tggctggaga gcgtgcgggc ttatgagaaa gcgattttga
    65281 gccagcgtgg gtaaacactg ccggatgcgg cgcgagcgcc ttatccggcc tacaggtcga
    65341 atgaccatgc gtgctgccac cctcaccccg gccctctccc tgaaagggcg agggagaaaa
    65401 acatagcctc gtgcggcaac ggagctgaac accgctccgg acagtcccct cgcccctacg
    65461 gggagagggt tagggtgagg ggaacaagcc gcaccatgtc taaacaaggc aacctaattt
    65521 acaggaacac agaacatgca aaacattacc cagtcctggt ttgtccaggg aatgatcaaa
    65581 gccaccaccg atgcctggct gaaaggctgg gacgagcgca atggtggtaa cctgacgctg
    65641 cgcctggatg acgccgatat cgcgccttat cacggcaact ttcatgcaca accgcgctat
    65701 attcccctca gccaacccat gcctttactg gcaaatacgc cgtttattgt caccggctcc
    65761 ggcaaattct tccgtaacgt ccagcttgat cctgcagcta acttaggcgt cgtaaaagtc
    65821 gacagcgacg gcgcgggcta ccatattctc tgggggttaa ccaacgaagc cgttccaact
    65881 tccgaacttc cggctcactt cctttcccac tgcgagcgca ttaaagccac caacggtaaa
    65941 gatcgggtga tcatgcactg ccacgccacc aacctgatcg ccctcaccta tgtacttgaa
    66001 aacgacaccg cggtctttac tcgccaactg tgggaaggca gcaccgagtg tctggtggta
    66061 ttcccggatg gcgttggcat tctgccgtgg atggtgcccg gcacggacga aatcggccag
    66121 gcgaccgcac aagagatgca aaaacattcg ctggtgttgt ggcccttcca cggcgtcttc
    66181 ggcagcgggt cgacgttgga tgaaacattc ggtttaatcg acaccgcaga aaaatcagca
    66241 caagtattag tgaaggttta ttcgatgggc ggcatgaaac agaccatcag ccgtgaagag
    66301 ttgatagcgc tcggcaagcg cttcggcgtt acaccactag ctagtgcgct ggcgctgtaa
    66361 ggagcaaaca tgatccgcaa agcctttgtc atgcaggtta accccgacgc ccacgaagag
    66421 tatcagcgtc ggcataaccc catctggccg gaactggaag cagtgttgaa atctcacggt
    66481 gcgcataact acgccattta cctcgacaaa gcgcgtaatc tgctgtttgc cacggtagag
    66541 attgaatctg aagaacggtg gaatgcggta gccagcactg atgtttgcca acgttggtgg
    66601 aaatatatga ccgatgttat gcccgctaac ccggataaca gcccggtgag tagcgagctg
    66661 caagaagtgt tttacctgcc gtaattgccc aatgtgccag agatgcctcc ggcacaatat
    66721 tttaaatttc gatttgaaaa attaaacata ttacatctcc cggtaagtta tatttccctg
    66781 atacattgtg agtaaatcac aaaaataatg aataacccat taatgattca tgtggtttat
    66841 ttaaataacc attatgtgca ttactccgca aatctgacct ttcactctgt ctcaaattgt
    66901 cattgtttac cgacatatcg cctctcatca cttaatcgct ccaccattac aattgattaa
    66961 aatatatttt atggagtccg attaatggca gctcttactg caagctgtat tgacctgaat
    67021 attcagggca atggcgctta ttccgttctg aagcagttgg cgacaatagc gttacaaaac
    67081 ggttttatca ccgactcaca ccagttcctg caaaccctgc tgctgcgcga aaaaatgcac
    67141 tctactggat ttggttccgg tgtcgccgtg ccgcacggta aaagcgcctg cgttaaacaa
    67201 ccgtttgtat tattcgcccg caaagcgcag gctattgact ggaaagccag cgatggcgaa
    67261 gacgtcaatt gctggatctg cctcggcgtg ccgcaaagcg gcgaagagga tcaggtcaaa
    67321 atcatcggca cactgtgtcg caaaattatc caccaggatt ttattcatca actgcaacag
    67381 ggcgacgccg accaggtgct tgccttgtta aatcaaaccc tcagctcata aggaagtggc
    67441 gatggagtca tccttatgta ttgtcgcgat caccaactgc cccgccggga tcgctcacac
    67501 ctacatggtg gcggaagccc tggaacagaa agcccgttct ctcggccata ccataaaagt
    67561 ggaaactcaa gggtccagtg gcgttgaaaa ccgcttatcc agcgaagaga ttgccgctgc
    67621 cgattacgtc attctcgcta ccgggcgtgg cctgagcggt gatgatcgcg cgcggtttgc
    67681 cgggaagaaa gtttatgaga ttgccatctc ccaggcgttg aaaaatatcg accagatttt
    67741 cagcgaatta ccgacaaact cgcagctttt tgccgcagat agcggcgtga agcttggcaa
    67801 acaggaagtg caaagcggca gcgtgatgag ccacctgatg gcgggcgtct ctgcggcgct
    67861 gccgtttgtc atcggcggtg gtatcctggt ggcgcttgcc aacatgctgg tgcagttcgg
    67921 tttgccgtat accgatatgt cgaaaggcgc gccgtcgttt acctgggtag ttgaatccat
    67981 cggttacctt ggcttcacct ttatgatccc catcatgggc gcttatattg cctcgtcgat
    68041 tgccgataaa ccggctttcg ctccggcgtt tctggtttgc tatctggcga acgacaaagc
    68101 gctgctcggc acccagtcgg gtgcgggatt cctcggcgcg gtggtgctgg ggctggcgat
    68161 tggctatttc gtcttctggt tccgcaaagt gcgccttggc aaagcattac aaccgctgct
    68221 cggttcaatg ctgatcccgt tcgttactct gctggttttc ggcgtgctca cttactacgt
    68281 tatcgggccg gtgatgtccg acctcatggg cgggctgctc cacttcctga ataccattcc
    68341 tccatcaatg aagtttgcgg cagcgtttct ggtcggtgcg atgctggcgt tcgatatggg
    68401 cggccccatc aataaaaccg cctggttctt ctgcttctca ctgctggaaa aacacattta
    68461 tgactggtac gccatcgtcg gcgttgtcgc gttgatgccg cccgttgcgg cgggtcttgc
    68521 gacgttcatt gcgcccaaac tgtttacccg acaggaaaaa gaggctgcca gtagtgccat
    68581 tgtggtcggt gccacagtcg caaccgaacc ggctatacct tacgctctgg cagctccgtt
    68641 gccgatgatt accgccaata cgctggcggg tggcattacc ggcgtgctgg tgatcgcttt
    68701 cggaatcaaa cgtctggcac ctggtttggg tatctttgac ccgctgatcg gcctgatgtc
    68761 gccggtgggt tcgttttatc tggtgctagc gattggcctc gtgctgaaca tttcattcat
    68821 tatcgtcctg aaaggattgt ggttacgccg caaagcgaaa gccgcgcaac aggagcttgt
    68881 ccatgaacat tgagttactg caacagttgt gcgaagccag cgccgtcagc ggcgatgaac
    68941 aggaagttcg cgacattctg ataaacacgc tggaaccttg cgttaatgag atcacctttg
    69001 atggtctggg cagctttgtt gcccgtaagg ggaataaagg tccaaaagtt gccgttgtcg
    69061 ggcatatgga tgaagtcggc tttatggtca cccacatcga cgagagcggt tttctgcgct
    69121 ttaccaccat tggcggctgg tggaatcagt caatgctcaa ccaccgggta accatacgca
    69181 cacacaaggg atttaaaatc cctggtgtga ttggttccgt cgcacctcat gcgttaacgg
    69241 aaaagcaaaa gcaacagccg ctgtcatttg atgagatgtt cattgatatt ggcgcgaaca
    69301 gtcgcgatga ggtggaaaaa cgcggtgtgg cgatgggcga ttttattagc ccggaagcca
    69361 attttgcctg ctggggcgaa gataaagtgg tcggcaaggc gctggataac cgcatcggct
    69421 gcgcaatgat ggctgaacta ttgcagacgg tgaataatcc cgaaattacg ctgtatggcg
    69481 tcggcagcgt ggaagaagaa gttgggctac gcggggcaca aacctcggcg gaacacatta
    69541 aaccggatgt ggtgattgtg ctggataccg ctgtcgcagg tgatgttccg ggcattgata
    69601 acattaaata cccgctgaaa ctgggccagg ggccggggct gatgctgttt gacaagcgct
    69661 acttccccaa ccagaaactg gtggcggcgt taaaaagctg tgccgcacat aacgatttac
    69721 cgctgcaatt ttccaccatg aaaaccggtg cgacggatgg cgggcgctac aacgtgatgg
    69781 gcggcgggcg tccggttgtt gcgctgtgcc tgccaacccg ctatctgcac gctaacagcg
    69841 gcatgatttc aaaagccgat tacgatgctc tgctcacgtt gatacgggat tttctgacca
    69901 ccttaaccgc ggagaaagtc aacgcgttta gccagttccg tcaggtggat taatgctaaa
    69961 cgaacgccag ttaaagattg tcgatctgct ggagcaacag ccgcgcacgc ctggtgagct
    70021 ggcgcaacag actggcgttt caggcaggac catcctgcgt gatattgact atctcaactt
    70081 tacccttaac ggcaaagccc gcattttcgc cagtggcagt gcgggctatc agctggaaat
    70141 cttcgagcgc cgcagctttt ttcagttgct gcaaaagcac gataacgacg atcggctgct
    70201 ggcgctgtta ttactgaata cctttactcc ccgtgcgcaa ctcgcctcgg cgcttaattt
    70261 gccagaaacc tgggtagcag agcgtctgcc ccggttaaaa cagcgttatg aacgcacttg
    70321 ttgcctggcc agtcgccccg gtttgggcca tttcattgat gagacagaag agaaacgcgt
    70381 tatcttgctg gcgaacttgc tgcgcaaaga tccgttttta attccgctgg cgggcgtaac
    70441 acgagacaat tttcagcatt tatccacggc ctgcgacaac caacaccgct ggccgctcat
    70501 gcagggtgat tatctctcca gcctgattct ggcgatttac gccctgcgta atcaactgac
    70561 cgatgagtgg ccgcaatatc ccggtaacga gataaaacaa atcgttgaac agagcggcat
    70621 gtttcttggt gataacgctg taagaaccct gacaggtttg atagagaaac agcatcagca
    70681 agcgcaggta atttcagccg atcatgtgct ggggttgctg caaagggtgc caggcatcgc
    70741 gtcattgaat attattgata cgcagctggt tgagaatatt accgggcatt tattacgttg
    70801 ccttgccgca ccggtgtgga ttgccgagca ccgccaaagc agcatgaata acctgaaagc
    70861 cgcctggcct gcggcctttg atatgagtct gcactttatt acgctactgc gtgaacagct
    70921 cgatattccc cttttcgata gcgatctgat cggtttgtat tttgcctgtg cgctggaacg
    70981 gcatcaaaac gaacgccagc cgatcatttt gctctcggac cagaacgcga ttgccactat
    71041 taatcagctc gccattgagc gcgatgtttt acattgtcgg gtgattattg cccgtagctt
    71101 aagcgagctt attgccattc gcgaagagat tgagccgtta ttgatcatta acaacagcca
    71161 ttatttactg gatgacgcgg taaataatta catcaccgta aaaaatatca ttacggctgc
    71221 cgatatcgaa caaataaaac actttctggc gacggcattt attcgccaac agccggaacg
    71281 ctttttctct gcccccggaa gttttcatta ttcgaatgta cgcggtgaaa gctggcaaca
    71341 tattacccgg caaatttgtg cgcaattagt ggcacaacac catattaccg ccgatgaagc
    71401 gcaacggatc atcgcccgcg aaggcgaagg tgaaaacctg attgttaatc gcctcgccat
    71461 cccacattgc tggagcgaac aggagcgacg ttttcgtgga ttttttatta ccctcgccca
    71521 accagttgag gtgaataacg aagtcattaa ccatgtcttg atcgcctgcg ccgccgccga
    71581 tgcgcgtcat gagctaaaaa tatttagcta tctggcaagc gtattgtgtc agcatccggc
    71641 agaagttatt gccgggttaa caggatatga ggcatttatg gagttacttc acaaggggtg
    71701 aactgtgtcg gatgcgccgc atccgacact gcatttatca tcaaaaacgt ttaacgcagg
    71761 ctattatagt catcaaccat ttcgttgtac tcgcgtaacg ctcgtggaaa tgagtcttcg
    71821 accatccagc ttgtgtttgc atcctgaaga tgctctttat cccagtcaga gtacgggact
    71881 ttatctctga cgcggcgaac atatttttta gtctcgagtt gatactggtc tgccgaatta
    71941 atgaacgaga aattcatacc gcctttatcc gcttctttgg cctgcgctac cagcgtttcc
    72001 agttcagaga ctttcttcat tgccgcatca acatcaaact tctcttgcga aatcaggtta
    72061 ttaatttgtt tcgctgaaat catgactgcc agagagtagt agtggaatgt tttcccttct
    72121 ctttcttcaa tatttttcag ttccgcaagc tggcgcttat cattaatttc ctgaatcgcc
    72181 gcatgataag attctgctac aggttcaaag gattccagat tttttaagaa tgtctggtgc
    72241 agcgttttcc ctttcgcaaa cgcatcgtct ttataatttt cctgggtgta atatttatcc
    72301 atttcgttaa tggtattacc caacgccacg gcagcatcaa tataactcac cgccacgcca
    72361 tcaataggtt gcagcgcagg cgttaacgcc acagcacttt ttacgccttt ttcacactca
    72421 gcgaggttgg attcactaat gccgtagatg ccataaacag tactctcttt accggtaggc
    72481 ccctgtttaa aatctttcag ccagtcagca taacgcgcca gactgttctg tacagggatc
    72541 tgtaacttgt tgtagcactt gatatagaca tccatttttt cgtcgaaaac cgcttgttca
    72601 tcgaccaccg gttgcgcagg tgtttcaggt ttagcttctg cttttgcaac gggagcttca
    72661 ggagctggtg cggcaggttg actattggca ggcgggggcg tttctgtttc ggctttttta
    72721 tcatcacaac ccgtcagacc gagggccatg atggcgacta taatagcgga agataataaa
    72781 t
//
LOCUS       DAZPEP010000005        72510 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZPEP010000005 DAZPEP010000000
VERSION     DAZPEP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.005"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUT9_000301"
     CDS             complement(<1..215)
                     /locus_tag="ACHUT9_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1346879.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUT9_000302"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="ACHUT9_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFR1346880.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUT9_000303"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="ACHUT9_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFR1346881.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUT9_000304"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="ACHUT9_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFR1346882.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUT9_000305"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="ACHUT9_000305"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFR1346883.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUT9_000306"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="ACHUT9_000306"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFR1346884.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUT9_000307"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="ACHUT9_000307"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFR1346885.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="ACHUT9_000308"
     CDS             complement(4604..5326)
                     /locus_tag="ACHUT9_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1346886.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUT9_000309"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="ACHUT9_000309"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1346887.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="ACHUT9_000310"
     CDS             6526..6744
                     /locus_tag="ACHUT9_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFR1346888.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUT9_000311"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="ACHUT9_000311"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1346889.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="ACHUT9_000312"
     CDS             complement(7478..7678)
                     /locus_tag="ACHUT9_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFR1346890.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUT9_000313"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="ACHUT9_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFR1346891.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUT9_000314"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="ACHUT9_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFR1346892.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="ACHUT9_000315"
     CDS             10175..12088
                     /locus_tag="ACHUT9_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1346893.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="ACHUT9_000316"
     CDS             12088..13110
                     /locus_tag="ACHUT9_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1346894.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="ACHUT9_000317"
     CDS             13104..13322
                     /locus_tag="ACHUT9_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFR1346895.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="ACHUT9_000318"
     CDS             13376..14245
                     /locus_tag="ACHUT9_000318"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFR1346896.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="ACHUT9_000319"
     CDS             complement(14300..14704)
                     /locus_tag="ACHUT9_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFR1346897.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="ACHUT9_000320"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="ACHUT9_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFR1346898.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGSR"
     gene            15689..17779
                     /locus_tag="ACHUT9_000321"
     CDS             15689..17779
                     /locus_tag="ACHUT9_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFR1346899.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUT9_000322"
                     /gene_synonym="dapC"
     CDS             complement(17846..19066)
                     /gene="argD"
                     /locus_tag="ACHUT9_000322"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFR1346900.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUT9_000323"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="ACHUT9_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFR1346901.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="ACHUT9_000324"
     CDS             complement(19747..20349)
                     /locus_tag="ACHUT9_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFR1346902.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="ACHUT9_000325"
     CDS             complement(20339..20506)
                     /locus_tag="ACHUT9_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFR1346903.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUT9_000326"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="ACHUT9_000326"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFR1346904.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUT9_000327"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="ACHUT9_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFR1346905.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUT9_000328"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="ACHUT9_000328"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFR1346906.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUT9_000329"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="ACHUT9_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFR1346907.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUT9_000330"
     CDS             25889..26695
                     /gene="nirC"
                     /locus_tag="ACHUT9_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFR1346908.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUT9_000331"
     CDS             26714..28087
                     /gene="cysG"
                     /locus_tag="ACHUT9_000331"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFR1346909.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28334..28501
                     /locus_tag="ACHUT9_000332"
     CDS             28334..28501
                     /locus_tag="ACHUT9_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFR1346910.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28796..30133
                     /gene="frlA"
                     /locus_tag="ACHUT9_000333"
     CDS             28796..30133
                     /gene="frlA"
                     /locus_tag="ACHUT9_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFR1346911.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30154..31176
                     /gene="frlB"
                     /locus_tag="ACHUT9_000334"
     CDS             30154..31176
                     /gene="frlB"
                     /locus_tag="ACHUT9_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFR1346912.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31226..32057
                     /gene="frlC"
                     /locus_tag="ACHUT9_000335"
                     /pseudo
     CDS             31226..32057
                     /gene="frlC"
                     /locus_tag="ACHUT9_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            32054..32839
                     /gene="frlD"
                     /locus_tag="ACHUT9_000336"
     CDS             32054..32839
                     /gene="frlD"
                     /locus_tag="ACHUT9_000336"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFR1346913.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32939..33670
                     /gene="frlR"
                     /locus_tag="ACHUT9_000337"
                     /gene_synonym="yhfR"
     CDS             32939..33670
                     /gene="frlR"
                     /locus_tag="ACHUT9_000337"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFR1346914.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33822..34907)
                     /locus_tag="ACHUT9_000338"
     CDS             complement(33822..34907)
                     /locus_tag="ACHUT9_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFR1346915.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(34919..36223)
                     /locus_tag="ACHUT9_000339"
     CDS             complement(34919..36223)
                     /locus_tag="ACHUT9_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFR1346916.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36235..36588)
                     /locus_tag="ACHUT9_000340"
     CDS             complement(36235..36588)
                     /locus_tag="ACHUT9_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFR1346917.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36599..37477)
                     /locus_tag="ACHUT9_000341"
     CDS             complement(36599..37477)
                     /locus_tag="ACHUT9_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFR1346918.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(37474..38700)
                     /locus_tag="ACHUT9_000342"
                     /pseudo
     CDS             complement(37474..38700)
                     /locus_tag="ACHUT9_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            complement(38700..39863)
                     /locus_tag="ACHUT9_000343"
                     /pseudo
     CDS             complement(38700..39863)
                     /locus_tag="ACHUT9_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            complement(39947..40309)
                     /locus_tag="ACHUT9_000344"
     CDS             complement(39947..40309)
                     /locus_tag="ACHUT9_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFR1346919.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40326..41231)
                     /gene="yhfZ"
                     /locus_tag="ACHUT9_000345"
     CDS             complement(40326..41231)
                     /gene="yhfZ"
                     /locus_tag="ACHUT9_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFR1346920.1"
                     /translation="MRRTFIKKEGVVIMTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="ACHUT9_000346"
     CDS             complement(41391..42395)
                     /gene="trpS"
                     /locus_tag="ACHUT9_000346"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFR1346921.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42388..43146)
                     /gene="gph"
                     /locus_tag="ACHUT9_000347"
     CDS             complement(42388..43146)
                     /gene="gph"
                     /locus_tag="ACHUT9_000347"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFR1346922.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="ACHUT9_000348"
     CDS             complement(43139..43816)
                     /gene="rpe"
                     /locus_tag="ACHUT9_000348"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFR1346923.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43834..44670)
                     /gene="dam"
                     /locus_tag="ACHUT9_000349"
     CDS             complement(43834..44670)
                     /gene="dam"
                     /locus_tag="ACHUT9_000349"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1346924.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44777..46063)
                     /gene="damX"
                     /locus_tag="ACHUT9_000350"
     CDS             complement(44777..46063)
                     /gene="damX"
                     /locus_tag="ACHUT9_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFR1346925.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="ACHUT9_000351"
     CDS             complement(46155..47243)
                     /gene="aroB"
                     /locus_tag="ACHUT9_000351"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFR1346926.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="ACHUT9_000352"
     CDS             complement(47300..47821)
                     /gene="aroK"
                     /locus_tag="ACHUT9_000352"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFR1346927.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="ACHUT9_000353"
     CDS             complement(48222..49460)
                     /gene="hofQ"
                     /locus_tag="ACHUT9_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFR1346928.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="ACHUT9_000354"
     CDS             complement(49372..49776)
                     /gene="hofP"
                     /locus_tag="ACHUT9_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFR1346929.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="ACHUT9_000355"
     CDS             complement(49766..50206)
                     /gene="hofO"
                     /locus_tag="ACHUT9_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFR1346930.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="ACHUT9_000356"
     CDS             complement(50190..50729)
                     /gene="hofN"
                     /locus_tag="ACHUT9_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFR1346931.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTD
                     NAEARVNTVLLQAEQQLARSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="ACHUT9_000357"
     CDS             complement(50729..51508)
                     /gene="hofM"
                     /locus_tag="ACHUT9_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFR1346932.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51628..54180
                     /gene="mrcA"
                     /locus_tag="ACHUT9_000358"
     CDS             51628..54180
                     /gene="mrcA"
                     /locus_tag="ACHUT9_000358"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFR1346933.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="ACHUT9_000359"
     CDS             complement(54345..54905)
                     /gene="nudE"
                     /locus_tag="ACHUT9_000359"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFR1346934.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55225..57360
                     /gene="igaA"
                     /locus_tag="ACHUT9_000360"
     CDS             55225..57360
                     /gene="igaA"
                     /locus_tag="ACHUT9_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFR1346935.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            57425..58093
                     /gene="yrfG"
                     /locus_tag="ACHUT9_000361"
     CDS             57425..58093
                     /gene="yrfG"
                     /locus_tag="ACHUT9_000361"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFR1346936.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58104..58505
                     /gene="hslR"
                     /locus_tag="ACHUT9_000362"
     CDS             58104..58505
                     /gene="hslR"
                     /locus_tag="ACHUT9_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFR1346937.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58530..59408
                     /gene="hslO"
                     /locus_tag="ACHUT9_000363"
     CDS             58530..59408
                     /gene="hslO"
                     /locus_tag="ACHUT9_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFR1346938.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59543..61243)
                     /locus_tag="ACHUT9_000364"
                     /pseudo
     CDS             complement(59543..61243)
                     /locus_tag="ACHUT9_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
     gene            61622..63244
                     /gene="pckA"
                     /locus_tag="ACHUT9_000365"
     CDS             61622..63244
                     /gene="pckA"
                     /locus_tag="ACHUT9_000365"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFR1346939.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKLDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="ACHUT9_000366"
     CDS             complement(63320..64672)
                     /gene="envZ"
                     /locus_tag="ACHUT9_000366"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFR1346940.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="ACHUT9_000367"
     CDS             complement(64669..65388)
                     /gene="ompR"
                     /locus_tag="ACHUT9_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFR1346941.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65616..66092
                     /gene="greB"
                     /locus_tag="ACHUT9_000368"
     CDS             65616..66092
                     /gene="greB"
                     /locus_tag="ACHUT9_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFR1346942.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            66189..68510
                     /locus_tag="ACHUT9_000369"
     CDS             66189..68510
                     /locus_tag="ACHUT9_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFR1346943.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            68948..69175
                     /gene="feoA"
                     /locus_tag="ACHUT9_000370"
     CDS             68948..69175
                     /gene="feoA"
                     /locus_tag="ACHUT9_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFR1346944.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69192..71513
                     /gene="feoB"
                     /locus_tag="ACHUT9_000371"
     CDS             69192..71513
                     /gene="feoB"
                     /locus_tag="ACHUT9_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFR1346945.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71513..71749
                     /gene="feoC"
                     /locus_tag="ACHUT9_000372"
     CDS             71513..71749
                     /gene="feoC"
                     /locus_tag="ACHUT9_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFR1346946.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            71952..>72510
                     /locus_tag="ACHUT9_000373"
     CDS             71952..>72510
                     /locus_tag="ACHUT9_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFR1346947.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
BASE COUNT        17434 a        19699 c        18347 g        17030 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccgaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggct ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt cgccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtcctgta atgaccttcg gtatattgca gaacattttt taaacttttt gttttatttt
    25861 ttgtttttat tttttaaagg ataatcaaat gtttacagac actattaata agtgtgcggc
    25921 taatgctgcg cgcattgcac gcctgtcggc aaataacccg ctcggctttt gggtcagctc
    25981 cgccatggcg ggcgcatatg tgggtcttgg gatcatcctg attttcacgc tcggtaattt
    26041 gctcgacccg tccgtacgtc cgctggtgat gggcgcgacc tttggtatcg ccttaacgct
    26101 ggtgattatc gccggttctg aactgttcac cggacacgcc atgttcctca cctttggggt
    26161 aaaagcgggc agcatcagcc acgggcaaat gtgggcaatc ctgccgcaaa cctggctggg
    26221 taacctggtc ggttccgtct tcgttgccat gctctatagc tggggcggcg gtagcctgct
    26281 gccggtagat accagcatcg ttcactccgt cgcgctggct aaaaccactg caccggcaat
    26341 ggtactgttc ttcaaaggtg cattgtgtaa ctggctggtt tgcctggcta tctggatggc
    26401 gctgcgcact gaaggggcgg cgaaatttat cgctatctgg tggtgtctgc tggcatttat
    26461 cgcgtccggc tacgagcact ccatcgctaa catgacgctg ttcgccctct cctggttcgg
    26521 caaccacagc gaagcctaca cgctggcggg tattggtcat aacctgctgt gggtgacgct
    26581 gggtaatact ttatcgggtg ccgtattcat gggattgggt tattggtatg ctacgccgaa
    26641 agcgaatcgt ccggttgcgg acaaatttaa tcaaactgaa acggctgccg gttaattact
    26701 aaggggtttt tacgtggatc atttgcctat attttgccaa ttacgcgatc gcgactgtct
    26761 gattgtcggc ggtggtgatg tcgcggaacg caaagcaagg ttgctgttag acgcaggcgc
    26821 tcgcttaacg gtgaatgcat tagcgtttat tccacagttc accgcatggg cagatgcagg
    26881 catgttaacc ctcgtcgaag ggccatttga tgaaagcctt ctcgacacct gctggctggc
    26941 gattgcagcg acggatgatg acacgcttaa ccagcgcgtc agcgaagccg ctgaagctcg
    27001 tcgcatcttc tgtaacgtag tcgatgcgcc gaaagccgcc agctttatta tgccgtcgat
    27061 tattgaccgc tcaccgctaa tggtagcggt ctcctctggc ggcacctctc cggttctggc
    27121 gcgcctgttg cgcgaaaaac tcgaatcact gctgccgttg catctgggcc aggtggcgaa
    27181 atatgccggg caattacgcg ggcgagtgaa acaacagttc gccacgatga gtgagcgtcg
    27241 ccgtttctgg gagaaattgt tcgttaacga ccgcctggcg cagtcgctgg caaacaacga
    27301 tcagaaagcc attactgaaa cgaccgaaca gttaatcaac gaaccgctcg accatcgcgg
    27361 tgaagtggtg ctggttggtg caggtccggg cgatgccggg ctgctgacgc tgaaaggact
    27421 gcaacaaatt cagcaggcag atgtggtggt ctacgaccgt ctggtttctg acgatattat
    27481 gaatctggta cgccgcgatg ctgatcgcgt tttcgtcggc aaacgcgcgg gataccactg
    27541 tgtaccgcag gaagagatta accagatcct gctgcgggaa gcgcaaaaag gcaaacgcgt
    27601 ggtgcggctg aaaggtggcg atccgtttat ttttggccgt ggtggcgaag agctggaaac
    27661 actgtgcaac gcgggtattc cgttctcggt ggttccgggt attaccgcag cttctggttg
    27721 ctctgcctat tcgggtattc cactcacgca tcgcgattat gcccagagcg tacgcttaat
    27781 taccggacac ttaaaaaccg gtggcgagct ggactgggaa aacctggcgg cagaaaaaca
    27841 gacgctggtg ttctatatgg gattgaatca ggccgcgact attcagcaaa agctgattga
    27901 acacggtatg cctggcgaaa tgccggtggc aattgtcgaa aacggaacgg cagtcacgca
    27961 gcgcgtgatt gacggtacgc tcacgcaact cggtgaactt gctcagcaaa tgaacagtcc
    28021 atcgctaatt attattggtc gggttgttgg cctgcgcgat aaactgaact ggttctccaa
    28081 ccattaattt aacccgggcc agagaattct ggccttctta atgactcttt ttatatattc
    28141 agcaaataaa acataacccc ttaataaata ttatctgcta atgggttata ttcaccgtta
    28201 cagcgactta caaaattaag tcattcgctc actgacgcca attaaatatt cctgtatcgt
    28261 tcttaacgag cagtctgctt atattcatat aatcaatgaa tattaattaa tatataatac
    28321 atagggaatg taaatgaaca aatttattaa agttgcactg gtaggtgcag tactggctac
    28381 gttaactgca tgtactggtc atattgaaaa ccgtgataag aactgctctt acgactacct
    28441 gctgcacccg gcaatttcta tttctaaaat cattggcggt tgcggtccta ctgcacagta
    28501 agtctctgac agaaatcggc taacaccggt ttaattgccc gacgccctct ggattttccg
    28561 agggcgtatt tgtttctatg tccttcttgc ccccgcccgt aaaaatattt tcactttaaa
    28621 ttcaatttga taactacatc cattagccac agcgcgatcc tgtgcgaaat tttgtgatct
    28681 tcctccacat tacataacat catatgttgt tatattcatc atgcattgtc atgttacctt
    28741 ttaaatgact gcaaactctc ccctacaacg tattggacaa gaaaaaggta tcgctatggg
    28801 aagccaggaa ctccaacgca agctcggatt ttgggccgtt cttgcaatcg ccgtcgggac
    28861 aaccgtcggc tccggtattt ttgtatctgt gggtgaagtg gcaaaagcag cgggcacgcc
    28921 gtggcttacg gtgctcgcgt ttgtcattgg cgggttaatt gtgatcccgc aaatgtgcgt
    28981 ctatgcggaa ctatccaccg cttatccgga aaatggcgca gattatgttt atctgaaaaa
    29041 tgccggaagc cgaccgctgg ctttcctctc cggctgggcc agcttctggg ccaacgatgc
    29101 accgtcattg tcgattatgg cgctggcgat tgtcagcaat cttggctttt taacgcctat
    29161 cgatccgttg ctcggtaaat ttatcgccgc cggattaatt atcgccttta tgttgctaca
    29221 cctgcgctcc gttgaaggcg gcgcagcgtt tcagacgcta attaccatcg ccaaaattat
    29281 cccgttcact atcgtcattg gccttgggat cttctggttt aaagcggaga attttgccgc
    29341 ccctaccacc actgcgattg gcgcaacggg cagctttatg gcgctgctgg cggggatctc
    29401 tgccaccagt tggtcgtata ccggcatggc ctctatctgt tatatgaccg gcgaaattaa
    29461 aaaccccgga aaaaccatgc cacgagcgct gattggttcc tgtctgctgg ttctggtgct
    29521 ctacaccctg ctggcgctgg tgatttccgg cctgatgccc ttcgacaaac tcgccaattc
    29581 tgaaacgccg atttccgacg ccctgacctg gatccccgca ctcggcagca ccgctgggat
    29641 ctttgttgcc atcacggcga tgatcgtcat tcttggttcg ctttccagct gcgtgatgta
    29701 ccagccgcgg ctggaatacg cgatggcgaa agacaacctg ttctttaaat gcttcggcca
    29761 tgtgcatccg aaatacaaca cgccggatgt ctccatcatc ctgcaagggg cgctggggat
    29821 cttcttcatc ttcgtttccg atctcaccag cctgctgggt tatttcaccc tggtgatgtg
    29881 tttcaaaaat accctcacct tcggctccat catctggtgt cgtaaacgcg acgattacaa
    29941 accgctgtgg cgtactccgg ctttcgggct gatgaccacc ctcgccattg cgtcaagcct
    30001 cattctggtc gcctcaacct ttgtctgggc accgattccc ggccttatct gcgccgtcat
    30061 cgttattgct actggtctgc ctgcttacgc cttctgggcg aagcgtagcc gccagctcaa
    30121 cgctttgtcg taatgttatc tggagaaaat aaaatgttgg atattgataa aagcaccgtg
    30181 gactttctgg tcaccgaaaa tatggttcag gaagtggaaa aagttctcag ccatgacgtt
    30241 ccgctggtgc acgccatcgt ggaagagatg gtgaagcgcg acattgatcg tatttatttc
    30301 gttgcctgcg gctcgccact caacgcggcg caaacggcga aacatctggc ggatcgcttt
    30361 tccgatcttc aggtctacgc catttccggc tgggagttct gcgataacac cccgtatcgc
    30421 ctcgacgatc gttgcgcggt gattggcgtt tctgactacg gtaaaacgga agaggtaatc
    30481 aaagcgctgg agctgggccg ggcctgcggc gcactcactg cggcgttcac caaacgcgcg
    30541 gacagcccga ttacctcggc ggcggaattt agcattgatt atcaggccga ctgtatctgg
    30601 gaaattcacc tgctgctctg ctacagcgtg gtgctggaga tgatcacccg cctcgcgccg
    30661 aacgcggaaa tcggcaagat caaaaacgat ctcaagccgt tgccgaatgc gctcggtcat
    30721 ctggtacgca cctgggaaga aaaaggccgc cagcttggtg aactggccag ccagtggccg
    30781 atgatttata ccgttgctgc gggtccgctg cgtccgctgg gttacaaaga aggcattgta
    30841 acgctgatgg aatttacctg gacgcacggc tgcgtgattg agagcggaga gttccgccat
    30901 ggcccgctgg agattgtcga accgggcgtt ccgttcctgt tcctgctcgg caatgatgaa
    30961 agtcgccaca ccaccgaacg cgccattaac tttgttaaac agcgtactga caacgtgatc
    31021 gtcatcgatt acgccgaaat ttcgcaaggg ctgcacccgt ggctggcacc gttcctgatg
    31081 ttcgtgccaa tggagtggct ctgctactac ctgtctattt acaaagatca caacccggat
    31141 gaacgccgct attacggtgg tctggtggaa tattaatccc tctcccggcc cggcaacggg
    31201 ccggatttac gcaaggagtt acccgatgaa aacaggtatg tttacctgcg gccaccagcg
    31261 gctgcctatt gaacacgcat ttcgtgatgc aagcgagctg ggttacgacg gcatcgaaat
    31321 ttggggcggt cgcccgcacg cgttcgcgcc ggacttaaaa gcgggcggca tcaaacaaat
    31381 caaggcgctg gcgcagacgt atcagatgcc gattatcggc tatacgccag aaaccaacgg
    31441 ctatccgtat aacatgatgc tgggcgatga acatatgcgt cgcgaaagcc tcgacatgat
    31501 caagctggcg atggatatgg caaaagagat gaacgcgggt tatacgctga tttccgcggc
    31561 ccacgcaggc tatctcacgc cacctaatgt tatctgggga cggctggcag agaacctaag
    31621 cgaactgtgt gagtacgcgg aaaacatcgg catggatcta atcctcgaac cgttaacgcc
    31681 gtatgaatcg aacgtcgtgt gtaatgctaa tgatgtgctt catgcgctgg cgctggtgcc
    31741 ttcgccgcgc ctgttcagca tggtcgacat ttgcgcgccg tatgtccagg cggaaccggt
    31801 gatgagttat ttcgacaaac tgggcgataa attatcgtca tctgcatatt gtcgacagcg
    31861 acggggccag cgacacgcat tacattcctg gagaaggcaa aatgccgctg cgggaactga
    31921 tgcgcgatat tattgagcgg ggctatgagg gttactgtac ggtggagctg gtgacgatgt
    31981 atatgaacga gcccagactc tatgcccgcc aggcgctgga acgctttcgc gcgctgctgc
    32041 cggaggatga gagatgaaaa ccctggcgac aatcggcgat aactgcgtcg atatctaccc
    32101 gcaactgaat aaagcgtttt ctggcggtaa tgcggtcaat gtggcggtgt actgcactcg
    32161 ctacggcata cagccgggat gcattacctt ggtgggtgac gatgactacg gcacaaagct
    32221 gaagcaggat ctcgcccgca tgggcgtcga tatcagccat gtccatacga aacacggcgt
    32281 taccgcacaa actcaggtgg aactgcacga caatgatcgc gtttttggcg actacaccga
    32341 aggcgtgatg gccgactttg ccctgagtga agaggattac gcctggctgg cgcagtatga
    32401 cattgtgcac gcggcaatct ggggacatgc ggaagacgca ttcccacagc tgcacgctgc
    32461 gggcaaactt accgctttcg acttctccga caagtgggac agcccgctct ggcagacact
    32521 ggtgccgcat ctcgattttg cctttgcctc cgcaccgcaa gaagacgaag cgctgcgtct
    32581 gaagatgaaa gcgattgttg cccgtggcgc aggaacagtg attgtcacgc tgggtgaaaa
    32641 cggcagcatt gcctgggatg gcgcgcagtt ctggcgtcag gctcctgaac cggtgacggt
    32701 tatcgacacc atgggtgccg gagattcgtt cattgccgga ttcctttgcg gctggtctgc
    32761 ggggatgaca ttaccgcagg cgatggcgca gggaacggcg tgcgcggcga aaaccattca
    32821 gtatcacggt gcctggtagg tataacgttg gcgtgagcat ctccgcgcca acgtgctgtt
    32881 acttgccgga aaacgaccct ataatccgtg taattcattc tttatttcag ggtcgattat
    32941 gtcagctacg gaccgctact ctcatcaact cctctacgct accgtccgcc agcggctgct
    33001 ggatgatatc gcgcaggggg tttaccaggc cgggcaacag atccctaccg aaaacgagct
    33061 ttgtacgcaa tataacgtca gccgcattac tattcgcaaa gctatcagcg acttagtggc
    33121 agacggcgta ctgatccgct ggcagggcaa aggcaccttt gtacaaagcc agaaagttga
    33181 aaacgccctg cttactgtca gtggttttac cgattttggc gtctcacaag gcaaggcgac
    33241 gaaagagaaa gtgatcgaac aggaacgggt cagcgccgcg ccgttttgcg aaaagctgaa
    33301 catccccgga aacagcgaag tgttccatct ctgccgggtg atgtatctcg ataaagagcc
    33361 gctgtttatt gatagttcat ggatcccgct gtcgcgttat cctgactttg atgagattta
    33421 cgtcgaagga agctccacct atcagttatt tcaggagcgt tttgacacgc gagtggtcag
    33481 cgacaaaaag accatcgata tctttgccgc cacccgcccg caggcaaaat ggctgaaatg
    33541 cgaactgggc gaaccgttgt ttcgcatcag caaaatcgcc tttgatcaga atgacaaacc
    33601 ggtgcacgtc tccgaactct tctgccgcgc caatcgcatc accttaacga ttgataataa
    33661 aagacattaa ccgtaggccg gataagatgc gccagcatcg catccggcga tgctggcgcg
    33721 ttgaatttta catcccgtac gttcccctca ccctaaccct ctccccaaag gggcgagggg
    33781 accgttcagt acggaattta gcttgacggt gctatccagc attaaatact ggcaatactc
    33841 tcacgcaaaa tccgcagcac cgtctcttca ccgttgcgat tcgggttaat gcgaatcgca
    33901 caatgttctg attgtggatt cgcctggcga aacgttccgg aaaggcgata aaagagcggc
    33961 gggatttcat atttcgactc tgcacccacc gggtaaggca aggcaccgcg cttttgcgcc
    34021 tcttccagca ctctggcggc aatcggctga tgaaactcga caatcaacac cttcgactgc
    34081 gcattagcaa tcaccgcgct tttcacttcc ggcaccgcac caccgttaag caaagccagc
    34141 aaccgttcag atacccccgc ctgcaccgcg tgcatcactg gcgcaaacac cagcccacac
    34201 aagacttcca gcgcctgagc gccctgaatc tggctgccgc cggaatagag cgtggagcga
    34261 atccgactaa taacatcggc atcgcctacc accgcgccaa caccctccgg cccaaacagc
    34321 ttgaagcagg aaaaagtcga gacatttgcc ccgcattcgc agccaattcg cgccactttc
    34381 atcaccgcat agttgtcatc ggttaatgca ggaacgccag ccgagcgcaa tgttgccagc
    34441 acatctgcca gaatgtagcc gtcctgcggc tgctggcgcg tatgctgcac cagcgccgca
    34501 tccggtagtt gctcgtcgac gacctgcttc agtgccgaca ggtcattgaa atcagcagta
    34561 ataagcgtca gccccatctg ctcaataatg acctgtgtcg tcgggtaaac aggcgcatca
    34621 tgtaccagaa gacgctgccc cggtttgagc aaagccgcca acgccgcacg aatcgcgcca
    34681 gtacccgcac cctgcaccag cgccgcagcc tgtgcgtgaa atgcatcagc cagcacctgt
    34741 tccacacgct gggtaacgcg cggttgattc agtcctggcg ttaagcctaa atcaccgcca
    34801 gtaagaaact cgctgccggg gaaatggcga caaatgctat ccaccagcgc aaactgcttt
    34861 tgctgcgcct caataatcgt cagactttgc agaggaaacg tcttcatagg tgccttcctt
    34921 aagccggaac gaacaggcca agccagtaaa gaacattcag cagaataccg gtgatcatta
    34981 ctgccacgac cggtgccgcc attttctgta ccggacggcc cagggattcg ttgaggaagt
    35041 aaatggcaac cgcgatagag aatccggtat aacccgccat cttaattgct gcgaaaatcg
    35101 aaccgaccag cagcgccact tccatcagca tattcatggc gttacggatg ttatccgacg
    35161 cattacgcac cgacgggtag cgtcccagcc atttgccgat cgaacgaagc agcaagactt
    35221 ccgccgaaat aaccactgcg cctaataccg ctgcaactat cggattcggc gagagatagc
    35281 ccaccgcata aacaaaggta aagcccgcaa ctgcatacac acccgttgct aaggcggtgg
    35341 tggcaatcaa cggtacaaaa cccagcccgc gcataaattc tgccagtgcc gcctggttga
    35401 tcagcgtttg cgattgttcc ggcgttacgc ctgcggagta cgctttctcc agagtgaaga
    35461 tcgacacttc actgccggcg aaaatcttca tgctggcaac ggcggcaatc aatgctccca
    35521 cgatggcgat atagggtaag tttttgataa tccgcgacgt gcgttcttca aacaccgaaa
    35581 gcccgctggc atcaatatca ttttcatcac gatgacgcag gtcgtgagtt atcgcgatcc
    35641 ccagcagcat caccatgcca ataaagattt cgatggattc agggttaaga tgtgggaaat
    35701 agcgcacgac taccacacgg gtcatcagaa caaccacggc ggcgaccaga ctttgcttcc
    35761 agccaaactg gtagaaaatc gccaccagcg ggaacagtgc aaaagcagaa accaccggcg
    35821 agcttaattc cccgaggcta cctaatacat ccaccggcag cgcggtcagc agctggttta
    35881 ctggcaacag gcaagtaagg atcaacacgc cccagatagc gccaagacca aacgccatca
    35941 ggctgtttat cgccagtacg ccgaggatat cggtaggaag aaagagtaac catgcgttga
    36001 gcagcccggt tttcagggta aaagagatcc ccaccgaggc cacaaaacca atgctcagac
    36061 caaaagcgat actccccgcc tcgcgacggt tcatatagcc ttcaatcagt tgcggcagga
    36121 tcgggcggat accgtcatga aaaacagccg ccgagcgatg cgccagaagc gatgtcatac
    36181 ccgtcaggca cgccaccacg ataatctgaa tatacagatc catagtcgtg ccttttattt
    36241 cagatggtta atcagcatcg gaatcgcgtg ttcgacgtgc tcaacggaaa ggccaaacgc
    36301 cacttttcct tcagcgatca ttttggcgat atgctcgtct ttcgctttaa tgcctggttt
    36361 ggcaatggtg caacttttgt tatagccaat caccgcgatg gcaatcgaca atgccgcgcc
    36421 cgcaccggta ttacacgcac cgatgtagta atccagttgc ccggatttca ctttcattgc
    36481 cgcttccatg tcgttgtgaa tgaaaacttc aaaactgcca ggagccgtcg cttcaatggt
    36541 ttttttaatc tgctcacgct gtaagcctgc aacgccaatc tttttcatga gtctgtcctt
    36601 attggaaaaa ttgagaggga gtttcacgta acatcacatc cacatcggcc tgactgaatc
    36661 ctgactggcg caattgcgga ataaaggtgg tcaataaata gtcatagcca taaccaccat
    36721 tggcttttaa atgggagcgg cgcgtaatat ccatcgacag catgacgcgg ttcagcaacc
    36781 cacggtcacg tagcgcgtgg agcatcgcaa tgcgcttttc gtccgggtag taactgttct
    36841 taccaatggt gtcgaactgc acgtacgcgc cgagatcgat catcttcaaa atgttgtcga
    36901 ggttgtcttt cagatcgcag tgaccaacgg tgacgcgcga aagatcaact ccgtgggctt
    36961 gcaacagagc cagttgttcc agccccatgg tgctgaacga cgtatgcgtg gagatcgggc
    37021 gtccggtctg gttatgcgcc agcgcagcgg caataaatac cttctcttcc agcggcgtaa
    37081 tctttccttc gctggagccg atctccgcga tgatcccggc tttcagctcc gtgccatcga
    37141 taccctgttc aatttcatcg accatctcct gcgccagttc ctgcacgctg cgggtcacca
    37201 catgttccgg gaaaaacgca tcctggtaat aaccagtaca ggccaccacg ttgatcccgg
    37261 tttcgcgcat tacaccgagc ataaattgcg cattgcgccc catgtaacgg ttggtcatct
    37321 caatcacatt acgtacaccc cgggtcatca ggtcgttcat ctcctggcaa atgaacgcat
    37381 actgatcaag gcggcagtcc acgttgtttt taaagccgga gagatcaata tgcagatgct
    37441 catgggccag ggtgtaaccc gtcggatcaa aactcatagg gtgtctcctg caaaccggag
    37501 ggaggaaaga aaagagcgac cattttgtgg cggtggcgca cggaaaaatt cacacacggt
    37561 agctcccaca tcagaaagcg tggtgcgcac accgagctgc gtagcagcca ggccttgctg
    37621 ataaaccagc agcggcacca cttcgcgggt atggtggctg tgaccaatgg tcggatcgtt
    37681 gccgtgatcc gccatcacga ccaggcaatc atctggctgc atcgcatcaa caagccgggc
    37741 aaggttacgg tcaacgacct gcaaacgttc ggcataacgt gcgacgtctt ctgcatgacc
    37801 agcgaggtcg gtttcctgga tattggtgca aataaacgcc gtcggatggg tgttaaattc
    37861 gtcgagggtg atatccataa tccgctggct atccaccagg ttttgccagc tcacgccata
    37921 aggattgctg acgatatctg ccaccttacc caccagcacg gttggcacgc ccgcctcata
    37981 cagtttttgc ggcacctgca cttttttatc gacaccgtag cccatatgca cgacctggaa
    38041 gccgttgtca taagcgccag aacgcggcgc attgatacca ataaagcgcc cttctttgct
    38101 ttctgcggca tcgagaatgc gttgactgtc ggttaacagg ccaccaaatg taatgacccg
    38161 accgacctgt acctgctcac gcacgatacg accaattttg attgcgtcgt caaaagagat
    38221 cacagagaga ttggcggtaa tgttatagac ttggcctaaa tccgcctcga gattatcgcc
    38281 aatcgcaacc gcctgattga cccacagaaa ttacagttca tcgccacggc gctccacctg
    38341 ccaaccggcg gatactaatg cctgctcaac acggtcaatc acatcgcgaa aaggcatccg
    38401 cagcggcggt aacgggcgcg tgcctaaaat ttcctgatgc cccataaagg tatcgccacc
    38461 ttcatgttgc agctctgcca cgccccaggt tgcggaatct gacggctgca tatcgcctgg
    38521 cgcataaccc aatgcgttga ttagccccag cttctccagc gttggtagct gcaaatgcgg
    38581 caactggctc aggatgtgac cacatgtatt cgctcccgca tcttgcggac gcaccagcgt
    38641 gacatctttc attgccccta cgccaaagct atcaatcact aacaccacaa atcgcgccat
    38701 tacgccccca gagagtttcc aagactgtca taacgaccaa ctattttcgg ttcgccacgg
    38761 tgaataccgg acaccagcac cacatcgcta cgggtgacaa aaatctgcgt gcggaaacag
    38821 agcaccactg cactgcttac cggaaactcg cctgccagcg acagggtgta gtcgatactg
    38881 ctgtcatcca cagttttgag attggtttca gtaatctttt gattttctgg cgtaaacacc
    38941 aacgcatgtt gcgcatgacc acgacggtag taaccgccgc cgtagcagta gctgtcgcca
    39001 cggaaatgat gggagatttc actcaaccag agcatcgcga tacgttcaga ctgatcgccc
    39061 tgctggtttg ccggaatagt gcccgtcagc gcatgaccgg gttcggcatg agtcacaccg
    39121 tattacgcca gtaatggcag cgaagtgcag ctggtcgctg aaggcgcgtt cagttgctca
    39181 agtgcaatac cagatttcgc cagttgatcc cgtgcctgta tcagcgtgtg aagattcggt
    39241 gtcggcaaaa cttttccggc agcctcatcc caaagcaggc aagggaaatg ggtaagtccg
    39301 gctaaatgca gccccggcag tttcttaatt tcagcaacca cctcgtgcag cgaatgctgt
    39361 acaaaaccgc tctcctgccc cggataaaga aaatcatcgt cgctataaac tttgagcagc
    39421 acaaactgca cacgtccggc cttcaccgct gccgcagaaa cttcccgcgc tttgtcgaga
    39481 gtaaacacgg tgatgacgtc ggtgccctgt tcaacggcgt cagcaacctg atgacaaggg
    39541 atttgtacca gatgcccctg atgtgccacc gacaaaccag cgcggcgcat gactcgcgcc
    39601 tctttgtaat ccaccgccac aatgccgctg taacctaatg ccagcaattt ttccgccagc
    39661 catgggttac ggccaaactg cttggtcatc agatacagtt caatgccgta aagccgcgcc
    39721 gtctcaatca gtcgcttacc gttttccagt acctgatcca cgtcgatcac ccagctgtcc
    39781 ggggcgatct tgccctgctg ccacaaactt agtgcggcgg aaatcagcgc cgggttctgg
    39841 cgtttcaatg cttctacaaa catcttgttg cctcgtttct gttccttgaa ttaaatattc
    39901 atttttttga atatttaggt tgtgcgacgt ggatttgtcg ggagcttcaa acctcttcgt
    39961 tagcagccat ccataatcca taaaggttgg ccagcaaata gccttcttca cacgggttaa
    40021 cttccagtgc gaattccttc aacaacacct gatgcaattg cacaacggct tgccagtggc
    40081 tggattgcgc cagctctgcc agcaattcgt tatccagcgg ctctatttct tcaccacggc
    40141 gactgcgcat cagtgcactc gccatatgtg tcatcgccat cgttccttgc tcactgcgca
    40201 ccggcagatg gcactctgtt tccagtacgt tgacgacctg catcatgcct ttgcagatgt
    40261 ccttatcaat aacgcctgcc tcgcaaagca ggttgagtct ggtttccata tcagatgcct
    40321 ttcgtttaat aactcggttc ctgttcccca ctcactaccc gttgttgatg ggcgagcagg
    40381 gcgtgtttat ctacaacggc acgcagcagt tgttgcattg ggtaatcatc gactcgcgtc
    40441 aggaccacgg cttcggtggc ctgtaaaaat cgcggatcgt ctgtgagcgg cgtcgcctct
    40501 aatcccagca tggtcagttc gttttccgcc accacgttcc agataaccgc atcgacatcg
    40561 cctttaacaa tgcgttgtaa actctcgtga taagagagat cgacacgttc cacatcacta
    40621 tcgccaaaaa agacatcggt catgattttc tgatccgccg aacggttatc cagccccacg
    40681 cgcttcacgt ttgcggactc gcctttacgg caaatcaact ggtgctcgcc aacgtaggtg
    40741 tgcggcccca actccagcgc gaggcataac cctttttgcg tgagataact ttccgccgcc
    40801 agtcgggaaa ccaccgccat gtcatacacg ccgttaagta aacactccac gcgaatatcc
    40861 gcgccgcgca tatgcgcata gtaaaaagga atgccatcaa actgagcttt cagcccgctc
    40921 gctaaacctt cgtacaagcg ggtatagggc aaaggcatcg cacataccac gttgttgata
    40981 tccacatgac tcagcaatgc tttgttatcc atctcgacca gataactgcc attgcgccca
    41041 cggcgttcaa tccgtatcgc tccgcttgat tccaacgttt tcaacgcggc ctgcgtcagg
    41101 ccaaccgatg aacggcattc agttgccagc tcatctatgg ttttcagtcg attaccgcat
    41161 ttttcaccca acaaataacg ggccagcgtc atgatgacga cgccttcttt tttgataaac
    41221 gttcgacgca taatatattt tcaataaaat gaatatttat atcttcatta aaatgaagat
    41281 aagtggcaac gacgagatgt ggaaacggcg aggcgcttca cattttttcg gattattgca
    41341 aatgaggata aaaaaaccgg gtttccccgg tttttggcat taatgttaaa ttacggcttc
    41401 gccacaaaac caatcgcttc gtacaccgct ttcagcgtac gggaagcgtg tacactggct
    41461 ttttctgcgc cgtctttcat cacctgttgc aggaaggctt catcgttgcg gaaacggtga
    41521 tagcgttcct gcaattcagt cagcataccg gaaacggcat cagccacttc acctttcaga
    41581 tgaccataca tcttgccttc gaactgtttt tccagttctg ggatgctctg gcccgttacc
    41641 gcagaaagga tatccagcag gttggaaacg cccgctttgt tctgcacatc gtagcgaact
    41701 accggcggct cgtcggagtc agtgaccgcg cgtttgattt tcttcactac cgatttcgga
    41761 tcttccagca ggccgataac gttattgcgg ttatcgtcag acttggacat cttcttggtc
    41821 ggctccagca gcgacattac gcgcgcgcca gatttcggaa taaacggctc cggcacctta
    41881 aagatctcgc catacagcgc gttgaaacgc tgggcgatat cgcgactcag ttccaggtgc
    41941 tgtttctggt cttcacccac cggtaccaga ttagtttgat acagcaggat gtccgcagcc
    42001 atcagcaccg gatagtcaaa cagaccagcg ttgatgttct cggcataacg cgcagattta
    42061 tctttaaact gcgtcatgcg actcagttcg ccgaagtagg tataacagtt cagtgcccag
    42121 cctaactgcg catgttctgg tacgtgggac tgaacaaaaa tggtgctttt ctcaggatcg
    42181 ataccacaag ccagatacaa ggccagcgta tccagcgtcg ctttacgcag cttctgtgca
    42241 tcctggcgca cggtgatcgc gtgttggtca acgatacagt aaatgcaatg gtagtcatcc
    42301 tgcatgttta cccactgacg cagcgcaccc atgtagttac caatggtcaa ttcacctgag
    42361 ggctgtgcgc cactaaaaac gatgggctta gtcatttttc gattcctgat tttcgctatg
    42421 cggaagccct aatgcgggca gaaggtcatt tatagactga taaattacat caggctggct
    42481 gagatcgata gcctcgccgt agttatatcc gtaggttaag ccaactgatg ggcaacctgc
    42541 cgcttttgcc gcctgaatat cattgcgtga gtcgccgaca aacagcatct gttgtggggc
    42601 aattcccatc cgctcagcca ccagtaacag cgggtccgga tgcggttttt tgttttgcac
    42661 atcatcgccg ccgataacca cgctaaagta tttggcaata tctaaggctt cgagcagcgg
    42721 tgcgacgaac ggcgtcggtt tgttggtgac caggcctagc ggcaggcctt tagcctgcaa
    42781 cgcgcccaac gtatcggcaa cgtgcgggaa caaaaacgtc ccctcttcgg caacctcgcc
    42841 atagtagcga tcgaacagtt tacgcagaat acgtacctgt tcttctgccg gaatgtcgtc
    42901 atcaacgggc ggtttaccca ttgttttacg ctgagtcgca cgttcctgac gcgcccaggt
    42961 caatgcgcgc tccatcagaa catctgcgcc gttaccaatc caggtaataa cgcgttcttc
    43021 acctgcgatg ggcaactcca gcgcatacag cgccatatct accgcagcag caagaccggg
    43081 agcactgtcg accagcgtac catcaagatc aaaagcgacg ccgcgaatat cttcaaactt
    43141 attcatgact tacctttgcc agttcactgc gcatttcatc aatgactttt ttgtagtctg
    43201 gctggtcgaa gattgccgaa ccggcgacga acatatccgc gcccgccgca gcgatttcgc
    43261 caatgttgtt caccttcacg ccaccgtcca cttccagacg aatgtcaaag ccagactcgt
    43321 cgatacggcg acgtacttcg cgcagtttat ccagtgtttg aggaatgaaa gactgaccgc
    43381 cgaaaccagg attgacggac atcagcagga tcacatccag cttatccatc acgtaatcca
    43441 gatagctcag aggtgtcgcc gggttaaata ccagacccgc tttacagcca ttttctttaa
    43501 tcagttgcag cgtgcggtca acatgctcgg aggcttctgg atgaaaggta atgatgctgg
    43561 caccagcggc agcgaaatca ggcacaatgc gatcgacggg tttcaccatc agatgtacgt
    43621 cgataggggc ggtaatgcca tagttacgca aggatttcag caccattggc ccaatcgtca
    43681 gattgggaac atagtggtta tccatgacgt caaaatgcac gacatcagcg ccagctgcca
    43741 gggcttttgc ggtatcttca cccaggcggg caaaatcagc cgacagaatt gagggggcaa
    43801 tcaaatactg tttcatccgc ttctccttga gaattatttt ttcgcgggtg aaacgactcc
    43861 tggtttgtac aaagccagca gttcgtccac ctttttacgt gtgccgccgt tgctgcttat
    43921 actgcgtcga actttgacga catgcaattt tgcgcgctga taccactcac gcgttaacat
    43981 cgtatcgtga ttggagatca gcactggaat atggcgctca accagacctt cggcgatctc
    44041 cgccagatgc gcttgttgtt caagcgtaaa actgtttgtg tgatacgccg taaagttggc
    44101 ggtcgcagac agcggtgcat aaggcggatc gcaatagacg acggatgcat catctgcgcg
    44161 cgccatgcta tcggcgtaag actcacaata gaaaaaggca ttctgcgctt tttcagcgaa
    44221 gtgatacaac tctgcttccg ggaaataggt ttttttgtag cggccaaacg gcacgttaaa
    44281 ctcaccgcgc agattgtaac gacacaggcc gttgtaaccg tagcggttca aatataaaaa
    44341 cagtaccgcc cgacggaacg gatcctggct tttgttgaac tcttcgcgga actgatagta
    44401 aacctcggcg caatttgttt cgggaacaaa cagctcgcgt gcggcctgta cgtactcatc
    44461 agtacgcatc ttcacaatgt tatagagact gatcaggtcg ctattgatat cggcaaggat
    44521 ataacgagaa aagtcggtgt tgagaaacac cgacccggca cctacaaaag gctcaaccag
    44581 acattcgccc ttgggcaaat gccgtttaat atcatcaagc aggggatact tgccccctgc
    44641 ccacttcaaa aaagcgcgat ttttcttcat gctgactaac taattacacc ttctccggct
    44701 gtggagaaag ctccgacagc atccagcgct tcaggcagct cccgcaacga taaaccattg
    44761 cgggagataa ccttgattac ttcagatcgg cctgtacctg acgcagcggt ttcgcccacg
    44821 ggtttttggc ctgaacatct gctggcaatg tagataccgc ttttttcgcc tcttctttcg
    44881 aagcgtacac gccagaaacc aggacatacc acggctgacc attacgcgcc gtttcataga
    44941 caacgtagtt tttcagattc tctttcttcg cccaaccgtt caggttgtcg tagttagagg
    45001 aactgctcag ctgcagagtg taatggctgg acggtgccga tttcaacgaa ccaacattac
    45061 ctgcggtctt ccctccagcg gctggtgttg ccgtggtttg cgccggggat gccgtctgta
    45121 ctggagccgt agtcgccgtc tcttttggtg ctggcgtaga agtcgcagtc ggtgccttcg
    45181 tgctcgccac aggggcagct ggttcagtac gcttcggcgt ctgtgctacc ggcttcggct
    45241 ccgttttcac ggttgcttgc ggttttttcg gttcaatcac cgcctgctga cgagctgggc
    45301 gcgtagtgga cggacgttca gcggtttgcg ttttcgccgt gtcacgcgat gcattgccat
    45361 tgcgaacagg cgcgacagtc gcgggttcgg tcggcaatgt ggaattgacc gccacattgt
    45421 tcaactgttg ctgattttgt ggctgggtca gcgcattgtt caggtcaccc tgcacttcaa
    45481 cacgttgttg accatccgtt gccgccgggg tttgcccttg agtcggcgta gaagagatcg
    45541 gtggcagaga aacatcctgc tgagtatttt ccgcagacgt ggttcccggc gctggctgca
    45601 ccccattcgc ctgatcggtc gcattgcctg caagatcaat actcttctcg ccagacgcgg
    45661 tttgatcgct ggaagaggtc gagggggctt ttagcgcaga accgataccg atgatcaaca
    45721 gcagtagaac cagaatgccg acgcccatca tcatatactg acgagaagcg ggtttactgg
    45781 ctgctttttt gcgcttacgc ggacgacgct ctacgcgctc ttcatccacg gtttcatctt
    45841 cggattcgtc aatttcttct tcgatttccg gttcctcatt gcgctctttt tgcgcacgag
    45901 tcggacggcg atcgtcagtg tcatcaagtt caatatcatc aaaattgatc tgcggttcgc
    45961 cacgttcagt acgctcagaa cgttcagaag attgacgaga acgaccagta cgacgatcgc
    46021 tgggatcggg tttcagctcg tcttctggtt tgaattcatc catttaacac cccactaaaa
    46081 ggttaatgct taccacgttg caattaacct gaagctaata gaccgcttga taagcggcct
    46141 gacctttctt gttgttacgc tgattgacaa tcggcaatgg cgttaagaac aagctcgtgc
    46201 gaaacgccgc tgcgaacttc actcttacca attgccaacg gaagaattaa gcgtatctct
    46261 cccgcaagga ctttcttgtc acgcagcata tgcggtaaat acgcctgcgc ggacatttcg
    46321 cgcggcccat tgaccggtaa cccagcccgc ttgagcaggg ttataatacg ctgcgtttcg
    46381 gcagaactaa actgcccgag acgttccgac gtccgcgccg ccatcaccat acccgcagcg
    46441 accgcttcac catgtaacca attgccatac cccatttcag cttcaatggc atgaccaaag
    46501 gtgtgtccca gattcagtaa agcacgtaac ccggtttcgc gctcgtcggc ggcgacaact
    46561 tctgccttca gttcacaaca acggcgaata cagtacgcca ttgccggacc gtccagacgc
    46621 aacaacgcat ccagattctc ttccagccag ttaaaaaacg caccgtcaag aataatgccg
    46681 tatttgatga cttctgccag ccccgacgct aactcacgcg ggggaagcgt tttcagacag
    46741 tcgagatcca ccaccactga agcaggctgg tagaacgcgc caatcatgtt tttaccgagg
    46801 ggatggttga ccgcagtttt gccgccaacg gaggaatcga cctgcgacag taacgtcgtc
    46861 gggacttgaa tgaaacgaac accgcgctga taactcgccg ccgcgaagcc ggtcagatcg
    46921 cccactacgc cgccgccaag cgccaccagc gtagtatcgc gaccatgcgg cttttgtaac
    46981 aacgccgtaa agacggtatc gagtacagcc aggcttttat actgctcgcc gtcagggagg
    47041 ataacgctat cgacattaac acccgcctgt tcaagtacgc cgcggacctt atcgagatac
    47101 agaggagcca gggtttcgtt ggtgaccaac atgacctgct cgcccgattt cagcggtaag
    47161 aatgaagctg gttcattaaa caaaccagat gcgatggtaa ttgggtaact acgttccccg
    47221 agagtaacaa caatcctctc cataacgcga catccacctt aattactgta cccgcagacg
    47281 agtgtatata aagccagaat tagttgcttt ccagcatgtg aataatctgg tttgcaacca
    47341 ctttagcgct ttgatcatca gtacgaatgg tcacgtcggc aatctcttca tacagcggat
    47401 tgcgttcatt ggccaacgct tccagaactt cacgtggcgg tgtttcaacg tgcagcaacg
    47461 ggcgtttttt atcacgctgc gtgcgtgcaa gttgcttttc gatggtcgtt tcaagataaa
    47521 cgacaacgcc acgagcggaa agacggttac gcgtttcacg ggatttcaca gagccaccgc
    47581 cagtagccag cacaataccc tgtttctcgg tcaactcatt gatgaccttt tcttcgcgat
    47641 cgcggaagcc ttcttcgcct tctaaatcga aaacccagcc cacatcagct ccggttcgtt
    47701 tctcaatctc ttgatcggaa tcgtaaaatt ccatattgag ttgttgagct aactggcgcc
    47761 caatagtgct ttttccggca cccataggcc caaccagaaa gatattgcgt ttctctgcca
    47821 ttttttcggt actactaaga ctattcgtta atgataaacc cgcttcgctc agagagcgcc
    47881 gcaggacatg aactgaaacc tcataagata ttgcgagagt cagactgaaa attatctcaa
    47941 tactccagcg ggtttggcaa ctgaataaat cacccatccc tctgcatatc tggtcgctgc
    48001 aagcgcgctg ccttgctacc accgctctgg cgataaatca ccgggtaaga ttagcgtaaa
    48061 aaagacagca aaatgccgcc tgaatgataa atcatcatca tggaacgcag gcagcgaaga
    48121 gcaccaactc tcaaatcggt actccttgta tgctaaatac ctgcgcgcgt caaatagatg
    48181 aaacacgttc agcgtaaaaa cattaccgct tttacggctg tttactcact ggaaaccagt
    48241 cgtggcgtga taaacaccac taactcgcgt cgttcatctt cttttccgtc atgacgaaat
    48301 aattgcccga accagggaat gtcgccaagc aacggtacgc tatcctgacc cgatttattt
    48361 ttacgggtaa aaatgccgcc cagcgccaac gtttctccgc ttttgacctc gacctgcgtt
    48421 tcgatctcct gcttatcaat cgccagcact tcgccatcgg cctgctgtag cacctgcccc
    48481 ggaacgttct ggctgatgtg taatttcagc cggatgcgac ctttttgtaa cactgtgggc
    48541 gtaacttcca tccccaggac ggcctcttta aattccaccg acgtcgcgcc actttccccg
    48601 ctggaaacct gatatggaat ttcgctcccc tgtttaatgc tggcaggctg aagatgtgag
    48661 gccagcagac gcggactggc gataatatcc agctgctgtt tttgttcgag cgcggaaagc
    48721 tcaagatcca gcaagcgtcc gttgatgcgc ccaatgttaa aaccgacatg cgttgtcgcc
    48781 gtcgctacgg agaggtcgct accaagcgtg gtgacttgcc caacgccacc agcgtgttgc
    48841 gcatcggcca gcgtccattt cacgcctaac tcacgcaaac ttttttcatt aatggtgaca
    48901 atatgcgccg acagctcaac ctgcccgacc ggcagatcca tttgcgctac ccactgttca
    48961 agcgcgctta acgccgtttt gttatctcgt agcaaaaggc gattggtgcg tttatcgacg
    49021 gtcatactcc ctttggcact cagtagcttc tcccccgctt tcgccagttc tcccgcgtcg
    49081 gcgtattgca gggttatact gcgattttcc agcggcagat ttgcctgcgc ccgcgcctgc
    49141 tccgcctcct ggcgggcgat attgttattc tgccaggcaa tggaatgcac tgagagaatg
    49201 ttgccttcct gccgcgttat cagtccggcg cttttcacta cagtttgtag tgcctgcttc
    49261 cagggaacat ctgttagatg taacgacacc gtaccgctga cgtctggcga cacgaccagg
    49321 ttcaacttct cctgttcagc cagcgcctgc aacacctgag ctaccggaac gtcatccacc
    49381 atcagcgtca ctttttgcgg ctttgccgcc tgtacgccgg gtatcagcat caacagtagt
    49441 gcggctatcc attgcttcat ttgtatctcc ttgccgttgc cacaaccatt gtggcggttc
    49501 gcagtttgtc ccggtaccca gcgttagtac gtctggcgtc agctgtaaaa ttgtccagcc
    49561 gttttccagc acatcgtttt gctgcacccg tcgccatttc ttttgcccgt cttttattac
    49621 accgatgatg cgctcgcctc gccctaccat cccctgatag cgccactggc taagttcgct
    49681 aatccggcat agatcttccg gcggtttaaa agggtcacgc ataccggtta aaaggcacaa
    49741 tacaatacct gccaacaacc agcgtttaac cctcatgcgg cgtctccagt tgtagcgtga
    49801 acaaaagatc atcaccttcc acgcttaatg aaaaacggct cacgctgacg ttgcgctctg
    49861 ccagccgtgt aaatgccgac ggcactgctt cccacagcgt tttcaacgcc aactcgcctc
    49921 cctgcgcgga tggatgccag gaaaccagtt gcgcgccggg caactgaaaa tccagtggcg
    49981 aaaagggcag cgttttttct tcgctgaagg gagtggtgtc aaccaggcga tacagtgtgg
    50041 cccactgctg gtgatgactt gcccgtagtc gaattaatgc gtcgcgctct tcatggtgtg
    50101 tcgatgacag aaaaatgagc gtaactaaca tcagcaacca gaatgcccag caaaactggc
    50161 ggaggcgggg tgatgtggcg aaccaccagt caaagaacat gttcatcgct aaccttcctt
    50221 gttaactgat actcaaattg ccagcgtccc tgcgcatcct gctgcgtggc tccccgctga
    50281 ttgagatgaa aagacgcatc ctggcggagt gacgtttcta gtgcgtttaa cgcggtaatg
    50341 cttgttgtca gccccttgat ctccagcgtt ccctgctgcc agcttatcgt tgtcagccag
    50401 gcgtgctcgg gtaaaagcgc cgccagtgct tccagcgcag attgccagtc gcgggtaaat
    50461 tgtcgctggc gctgccgttg caaacgctgc tcacgaattt gttggcgctc cagcaaacgt
    50521 ggtttcgtta tctgtaagct gcgggcgagt tgttgttccg cctgaagcaa aacggtgttt
    50581 acgcgtgctt cggcgttgtc tgtcagacgc agtattagcg ttatcccgac ggccagcagc
    50641 agaggcgcaa cgaacatcag caaccagaaa cgaagaaaag cggtccggcg ttgctgtcgc
    50701 cagggcaaaa aattaattgg cgggttcatc agtacgcctt cccaagcgcc agccccagcg
    50761 cgatggcaaa gtctccacct ggcggcggta gcggcggctg acgaacagaa acggcctccc
    50821 agggatcaaa tccgccttcg ccacatatcg cgacgctttc gggatcaacg gataacgctg
    50881 ccgccagctc cttcgcgcta gtcatcccca ccgccagttt gcgcccccag cgacagcgtg
    50941 tcgcccacag ccactgttcg ttatcacgcc aggccagaca ttgctgatga gaaggtaaaa
    51001 aaggcaggaa tcgctgtaat gcactggcat ccggggtgat cgcgctcaca tgaacacgca
    51061 acctttctgc cagagtgagt agcgttgcca gctctttgct ttgcgcggca gtcacgttat
    51121 aggcgggact gagtgagtct tcgctgtaat cgaaacgcag ggagtccgga tccatatcca
    51181 gctcgcgggc catcgtccct gacagccagg ccgtttgctc ccgctcaccg agggacatcg
    51241 acgggcgcgg aaatgaccgc tgtaatgtgc gactggcggg aaacgccaac ataatgtgat
    51301 gacgctgcgg cagttcgcga ctccacggta acaacgtttt agccagccgc tgcgcatcaa
    51361 caatccgccc atctttgata atgtcgttct ccagcggcaa ccgccaccag cgttgcaaaa
    51421 agcattcttt tgcgccccgc acgatcgcaa ccgctactgc ttcttgctgt tgtaaatgca
    51481 aaccaatttg ccagatctta aatgccattg tgatgatctc cttatcaccc gtcactctga
    51541 cgggtatatc aatgcgtctg gcttgccttt atactaccgc acgtttgttt ataaactgcc
    51601 caaatgaaac taaatgggaa atttccagtg aagttcgtaa agtatttttt gatccttgca
    51661 gtctgttgca ttctgctggg agcaggctcg atttatggcc tataccgcta catcgagcca
    51721 caactgccgg atgtggcgac attaaaagat gttcgcctgc aaattccgat gcagatttac
    51781 agcgccgatg gcgagctgat tgctcaatac ggtgagaaac gtcgtattcc ggttacgttg
    51841 gatcaaatcc cgccggagat ggtgaaagcc tttatcgcga cggaagacag ccgcttctac
    51901 gagcatcacg gcgttgaccc ggtggggatc ttccgtgcgg caagtgtggc gctgttctcc
    51961 ggccacgcgt cacaaggggc gagtaccatt acccagcagc tggcgagaaa cttcttcctc
    52021 agtccagaac gcacgctgat gcgtaagatt aaggaagtct tcctcgcgat tcgcattgaa
    52081 cagttgctga cgaaagacga gatcctcgag ctttatctga acaagattta cctcggttac
    52141 cgcgcctatg gtgtcggtgc tgcggcacaa gtctatttcg gtaaaacggt cgaccaactg
    52201 acgctgaacg aaatggctgt gatagccggg ctgccgaaag cgccttccac ctttaatccg
    52261 ctctactcaa tggatcgtgc cgtcgcgcgg cgtaacgtcg tactgtcgcg gatgctggat
    52321 gaagggtata tcacccaaca acagttcgat cagacacgca ctgaggcgat taacgctaac
    52381 tatcacgcgc cggagattgc tttctctgcg ccgtacctga gcgaaatggt gcgccaggag
    52441 atgtataacc gttatggcga aagtgcctat gaagacggtt atcgcattta caccaccatc
    52501 acccgcaaag tgcagcaggc cgcacagcag gcggtacgta ataacgtgct ggactacgac
    52561 atgcgccacg gctatcgcgg cccggcaaat gtgctgtgga aagtgggcga gtcggcgtgg
    52621 gataacaaca agattaccga tacgctgaag gcgctgccga cctatggtcc gctgctgcct
    52681 gccgcagtca ccagcgccaa tcctcaggaa gcgacggcaa tgctggcgga cgggtcgact
    52741 gtcgcattga gtatggacgg cgttcgctgg gcgcgtcctt accgttcgga tactcagcag
    52801 ggaccgacgc cgcgtaaagt gaccgatgtt ctgcaaacgg gtcagcaaat ctgggttcgt
    52861 caggttggcg atgcatggtg gctggcacaa gtgccggaag tgaactcggc gctggtgtcg
    52921 atcaatccgc aaaatggtgc cgttatggcg ctggtcggtg gctttgattt caatcagagc
    52981 aagtttaacc gcgccaccca ggcactgcgt caggtgggtt ccaacatcaa accgttcctc
    53041 tacaccgcgg cgatggataa aggtctgacg ctggcaagta tgttgaacga tgtgccaatt
    53101 tctcgctggg atgcaggtgt cggttctgac tggcagccga agaactcacc accgcagtat
    53161 gctggtccaa ttcgcttacg tcaggggctg ggtcagtcga aaaacgtggt gatggtacgt
    53221 gcaatgcggg cgatgggcgt cgactacgct gcagaatatc tgcaacgctt cggcttcccg
    53281 gcgcaaaaca ttgtccacac cgaatcgctg gcgctgggtt cagcgtcctt caccccaatg
    53341 caggtggcgc gcggctacgc ggtcatggcg aacggcggct tcctggtgga cccgtggttt
    53401 atcagcaaaa ttgaaaacga tcagggcggc gtgattttcg aagcgaaacc gaaagtagcc
    53461 tgcccggaat gcgatattcc ggtgatttac ggtgatacgc agaaatcgaa cgtgctggaa
    53521 aataacgatg ttgaagatgt cgctatctcc cgcgagcagc agaatgtttc tgtaccaatg
    53581 ccgcagctgg agcaggcaaa tcaggcgtta gtggcgaaga ctggcgcgca ggagtacgca
    53641 ccgcacgtca tcaacactcc gctggcattc ctgattaaga gtgctttgaa caccaatatc
    53701 tttggtgaac caggctggca gggtaccggc tggcgtgcag gtcgtgattt gcagcgtcgc
    53761 gatatcggcg ggaaaaccgg taccactaac agttcgaaag atgcgtggtt ctcgggttac
    53821 ggtccgggcg ttgtgacctc ggtctggatt ggctttgatg atcaccgtcg taatctcggt
    53881 catacaacgg cttccggagc gattaaagat cagatctcag gttacgaagg cggtgccaag
    53941 agtgcccagc ctgcatggga cgcttatatg aaagccgttc ttgaaggtgt gccggagcag
    54001 ccgctgacgc cgcctccggg tattgtgacg gtgaatatcg atcgcagcac cgggcagtta
    54061 gctaatggtg gcaacagccg cgaagagtat ttcatcgaag gtacgcagcc gacacaacag
    54121 gcggtgcacg aggtgggaac aaccattatc gataatggtg aggcacagga attgttctga
    54181 ttaaaaaggc gcttcggcgc cttttcagtt ggctgacaaa gtgcatttgt ttatgccgga
    54241 tgcgggtgaa cgcgttatcc ggctaacaaa atcgtgaaaa atcaatatat tgcaggaacc
    54301 ccataagcct gataatcgtt gtgcatcagg caaacttcac gcatttacac tcgcccctgc
    54361 cctttcaacc attcgcgcac gaggaacagc gcgctgacat tacgcgcttc gttgaagtca
    54421 gggtcttcca gcaaatccat catatgcgcc agcggccagc gcacctgtgg tagcggctct
    54481 ggctcatcgc cttccagcga ttccgggtag agatcttgcg cgaccacgat attcattttg
    54541 ctggaaaagt aagacggtgc catgctgagc ttcttcaaaa aagtcagatc gttcgcacca
    54601 aatccaacct cttcttttag ctcgcggtta gcggcttcgt agacgctttc acccggatca
    54661 attaatcctt tcgaaaaacc taattcgtag gattcagttc ccactgcgta ttcgcggatc
    54721 aggatcaggt gatcgtccac aatcggcaca atcatcactg cttcccggtt ggttggacgc
    54781 attcgttcat aaacacgccg cacgccattg ctgaactcca gatccacgct ctcgacggta
    54841 aacagtcggg aatgggctac agtttcaaca ttcagaatgg tgggtttttg taatgatttg
    54901 ctcatcgtgg gatctatgct gtgaaatcag cggttattgt gcgatatcgg acacgctttc
    54961 ggcaatgtga attgcatgtt atttacattt atgtaactta ataaataatt gtcctcaaat
    55021 caaattaaaa gtcaataggt tgaaataact ccaggaattt gctgatatcc cgccttcaga
    55081 gggtttgcta tgatcagcgg ttactgtgat gtgcttaatg atgctcaagt taaactccac
    55141 gcttgccgat agccaaccgc agaatcatgt attgtgtctg gtgcgactga ccacgcctga
    55201 cagactaagt aagatgggga aagcatgagc accattgtga tttttttagc tgctttgctg
    55261 gcctgctcac tacttgcggg atggctgata aaagtgcgat ccagacggcg tcagctgccc
    55321 tggaccaacg ccttcgcgga tgcgcaaacg cgtaaactca cacctgaaga acgtagcgcc
    55381 gttgaaaatt atcttgagag cctgacgcag gtattacagg tgcctggccc aacgggagct
    55441 agcgcggcac cgatctctct ggcgctgaat gccgaaagca acaacgtcat gatgctgaca
    55501 cacgctatca cgcgttacgg catctctacc gacgatccga ataaatggcg ttactacctc
    55561 gattcggtag aagtccacct gccccctttc tgggaacaat acatcaacga tgagaatacc
    55621 gttgaactga tttataccga ttcgctgccg ctggttattt cactcaacgg tcatacgctg
    55681 caggagtaca tgcaggaaac gcgcggctat gccttgcaac ctgttccgtc cacgcaggcg
    55741 tcgattcgcg gggaagagag tgagcaaatc gagctactca atattcgcaa agaaacgcat
    55801 gaagaatatg cgctgagtcg tccgcgcggg ctgcgtgaag cgttgctgat cgtcgcctcc
    55861 ttcctgatgt tctttttctg cctgattacc ccggatgtat ttgttccgtg gctggcaggc
    55921 ggcgcattac tgctgctggg cgcaggtctg tgggggctat tcgcgccccc ggcaaaatcc
    55981 tccctgcggg aaattcattg tctgcgcggt acaccccgtc gttggggatt gtttggcgaa
    56041 aacgatcagg aacagatcaa caatatttca ctcggtatta tcgacctggt ctatcccgca
    56101 cactggcagc catacattgc tcaggatctc ggtcaacaaa ccgatattga tatctatctc
    56161 gaccgccacg tagtgcgtca ggggcgatat ctttcgctgc atgatgaagt aaaaaacttt
    56221 ccgttacagc actggctgcg cagtacgatt atcgctgcgg gctcgctgct ggtgctgttt
    56281 atgctgttat tctggatccc gctggatatg ccgctgaaat tcactctctc atggatgaaa
    56341 ggcgcacaga ccattgaagc caccagcgta aaacaactgg ctgacgctgg cgtgcgggta
    56401 ggcgatacat tgcgtatcag cggtacggga atgtgtaata ttcgcacttc cggaacctgg
    56461 agcgcgaaaa ccaattcacc ttttttaccg tttgactgct cgcagatcat ctggaacgac
    56521 gcccgctcat tgccattacc agaatctgaa ctggtcaaca aagcgacggc attgactgaa
    56581 gcagttaatc gccagctgca ccctaaaccg gaagatggat ctcgcgtcag tgcctcatta
    56641 cgttcagcaa ttcaaaaatc cggcatggta ttgcttgatg attttggcga cattgtactg
    56701 aagacagcgg atttatgttc tgccaaagat gactgtgtgc gactgaaaaa tgcgctggtc
    56761 aatctcggca acagtaaaga ctgggacgcg ctggtaaaac gcgccaacgc cgggaagctc
    56821 gatggcgtga atgtgttatt acgcccggtg agtgcggaat cgctggataa cctggtggca
    56881 acctccaccg cgccgtttat cacgcatgaa acggcgcgag cggcacaatc actaaacagt
    56941 ccggcccccg gcggattcct gattgtcagc gacgaaggca gcgattttgt tgatcagccc
    57001 tggccttcgg catcacttta cgactacccg ccgcaagaac agtggaacgc tttccagaaa
    57061 ctggcacaaa tgctgatgca tacgccgttt aacgccgagg gtatcgtcac aaaaatcttc
    57121 actgacgcca atggtacgca gcatattggc cttcatccaa tcccggatcg ttccggcctg
    57181 tggcgctatc tcagcaccac attgctgcta ctgacgatgc tgggtagcgc catttacaat
    57241 ggcgtacagg cctggcgtcg ttaccagcgt catcgcactc gcatgatgaa gattcaggcc
    57301 tattatgaaa gctgcctgaa cccgcaactg atcacccctt cagaaagcct tatcgaataa
    57361 cacgtttgcg cggcaggtta tgctaccctg tcgcgcaaat tgcttcactc tggagatttc
    57421 cctcatgcat atcaacattg cctggcagga cgtagatacc gttctgctgg atatggacgg
    57481 cacgttgctc gacctcgcct tcgataacta tttctggcaa aagctggtgc ctgaaacatg
    57541 gggcgcgaaa aacggggtta cgccacagga agcgatggaa tatatgcgcc agcaatatca
    57601 cgacgtacag catacgctaa actggtactg tcttgattac tggagtgagc aactgggtct
    57661 ggatatctgt gcgatgacca ccgagatggg accgcgtgcc gtactgcgtg aagataccat
    57721 tccgtttctt gaggcactga aagccagcgg taagcagcga attttgctca ccaatgcgca
    57781 tccgcacaac ctggcggtaa aacttgagca taccggtctg gacgcacacc ttgatttatt
    57841 actttccacc cacacatttg gttatccgaa agaggatcag cggttatggc atgcggtggc
    57901 cgaagctacg ggtctgaaag ctgaaagaac gctgtttatt gatgacagcg aagcgattct
    57961 cgatgctgcc gcgcaatttg gtattcgtta ctgcctcggc gtgactaatc ctgattccgg
    58021 gattgccgag aaacagtatc aacgccatcc gtcactgaat gactaccgcc gcctgatccc
    58081 ctcgctaatg tgaaggagac gccatgaaag aaaaacctgc tgttgaggtt cgactggata
    58141 aatggctatg ggctgcccgt ttttataaaa cccgcgcgct ggcccgtgaa atgattgaag
    58201 gcggtaaggt gcattacaac gggcagcgca gcaagccgag caaaatcgtc gagctgaatg
    58261 ccacgctcac tctgcgccag ggaaatgacg aacgcacggt gattgtaaag gcgattactg
    58321 aacagcgtcg ccccgccagc gaggcagcct tgctgtatga agagactgcg gaaagtgtag
    58381 agaaacgcga aaaaatggcg ctggcacgta aacttaatgc cttaaccatg ccgcacccgg
    58441 accgacgccc ggacaaaaaa gagcgccgcg acctgttacg atttaaacac ggcgacagtg
    58501 aataactgtc acctgcaaga gagatgatta tgccgcaaca tgaccaatta catcgctatc
    58561 tgtttgaaaa ctttgccgtg cgcggcgaac tggtaaccgt ttcggaaacc ctgcaacaga
    58621 tccttgagaa tcacgattat ccgcagcccg ttaaaaacgt gctggcagaa ctgctggttg
    58681 cgaccagcct gttaaccgct acgctgaagt ttgatggtga tatcaccgta cagctgcaag
    58741 gcgacggtcc gatgaatctg gcggttatta acggtaacaa taaccagcaa atgcgcggtg
    58801 tggcgcgcgt gcagggcgaa attccagaaa atgccgacct gaaaacgctg gtcggcaatg
    58861 gttacgtggt gatcaccatt accccgagcg aaggcgaacg ctatcagggc gtggttggtc
    58921 tggaaggtga taccctggcg gcctgcctgg aagattactt tatgcgttct gaacagctgc
    58981 cgacgcgcct gtttattcgc accggcgacg tagacggcaa accggctgca ggcggtatgt
    59041 tgttgcaggt aatgcctgcg caaaatgccc agcaggacga ctttgaccac ctggcgacgc
    59101 taaccgaaac catcaaaacc gaagaactgc tgaccttacc ggcaaacgaa gtgttgtggc
    59161 gtttgtatca cgaagaagag gtgacggttt acgatccgca ggatgtggag ttcaaatgca
    59221 cctgctcgcg tgaacgttgc gccgacgcgc tgaaaacgct gcctgatgaa gaagtcgata
    59281 gcatcctggc ggaagatggc gaaattgaca tgcattgtga ttactgcggt aaccactatc
    59341 tgttcaatgc gatggatatt gctgaaatcc gcaacaacgc gtctccggca gatccgcaag
    59401 ttcattaatg agtatgtccg gcagagaggg ctctgccgga ttttacgacg taatggcctg
    59461 atgtgctgcg cttaccaggc ctgtgaaata aatcgcaaat tattgttggt cagatacagc
    59521 gtcgtatccg ttaaaagagt tgtcacttac ttaagtttat ttccagcgtt tcaccatcta
    59581 ccgtcagatc cagttttcca tccttcgcag ctgtgagtaa tttttctttc gttatttcgt
    59641 tcggaagtaa actcatatca agcgcgtccc attcttttct gtctgagtca aagccataga
    59701 caatgactct gtcatcatta aaagcaaaca ggatccgctc cgcctgacca tcactattca
    59761 gatcttgctc gaccaggacg caggcgtctt tttcaataca ggtcatcacg ttatagcgat
    59821 cctggattaa ggccgaccag aatgtcgcat ccggtttaac agaaccagga gcaattaaca
    59881 cattttcggc taatactttt tccgatacct gttcctgaag atgttgctct ccatctaacg
    59941 ccatcagcag atcccgcgcg cgtttcgggt ctttcataaa cccggcatcg cttttcagcg
    60001 actcaagcgc cgcacgtcca tagcgaccgc tctgttcgag catgtagata gttacctggt
    60061 ctggcgtgtt tttgccgctc tgataacgcg ccatatgact gttcacgcta atgcgcatac
    60121 tgtccagcac cggcgaatta agaagcacca gtatcaccaa caccaataac gaaaccgcaa
    60181 ggttcacttt gccctgaaga acaaggggat tttgcccatt acgccagacg atactgacaa
    60241 aataccccag cgaccacacc agtaacacca gcaccgccag cgcgccctgc aagcggtcga
    60301 cagtccagcc gtactgagcg acccgcagcc ataacgccca ggcggcgacg aacacataca
    60361 gcggagcaac cagcaaagcg gttttaatca ggcaacgcaa cggccccgtc cagggaagtg
    60421 acgctttttg cggatcgcgg acaatagcca ttaagatcaa ttgcaaaaag gccagcgtta
    60481 acagcaaccc ggcggcggag atgtggcgag aaatcgcgct caggtccgta aacggcaggg
    60541 tgatgataaa catcagggtt agcaatgata ctaacggcag caaccccgtg gcgattagcg
    60601 tgaacaactt ttgaatagag tcgattaaac gtgactgtgt acgcgcgagg atcaccacca
    60661 gtgcggtaac cagaccaaat gtaagataca taaaccagtc ggttgcaaaa aagagcgtat
    60721 tgaaaaacgt gatgccaaca agtttaaaca actcacccca cagtaaaagc accagccacg
    60781 tcaggccatt ggagagaaaa atcaccagta atataagtac gttatgccat actgactggt
    60841 aaaagtagct atagcggcta ctgccattgc ggatacgaag gctttgttgt atccacggta
    60901 gcaacaacat tgccatcaac agcagatatc agccaaaatc ccaaatcgcc ttttcagctc
    60961 tccaggggtt catgccatca gtttgccact tcagccaacc gctcatccct gtcgtggcaa
    61021 taaacaccag cgccagccat ccccaaaggc gcttctgttt aaaagagatc acggagaaga
    61081 gtaaaacgga tgaaaacgcg acggtcgcag gcacgccata aacaatccaa ctgtgatttt
    61141 ttccggcaag ccaggcgata agcaggtagc aaaccagccc ctgaagtaat ccggtagcaa
    61201 tcatccccca gcgtgttgcc ggtgaaagtt caacgttatc catgttggtt atccagaatc
    61261 aaaaggtggg ttaattatcg catccgggca gtagtatttt gcttttttca gaaaataatc
    61321 aaaaaaagtt agcgtggtga atcgatactt taccggttga atttgcatca atttcattca
    61381 ggaatgcgat tccactcaca atattcccgc catataaacc aagatttaac cttttgagaa
    61441 cattttccac acctaaaatg ctatttctgc gataatagca accgtttcgt gacaggaatc
    61501 acggagtttt ttgtcaaata tgaatttctc cagatacgta aatctatgag ccttgtcgcg
    61561 gttaacaccc ccaaaaagac tttactattc aggcaataca tattggctaa ggagcagtga
    61621 aatgcgcgtt aacaatggtt tgaccccgca agaactcgag gcttatggta tcagtgacgt
    61681 acatgatatc gtttacaacc caagctacga cctgctgtat caggaagagc tcgatccgag
    61741 cctgacaggt tatgagcgcg gggtgttaac taatctgggt gccgttgccg tcgataccgg
    61801 gatcttcacc ggtcgttcac caaaagataa gtatatcgtc cgtgacgata ccactcgcga
    61861 tactttctgg tgggcagaca aaggcaaagg taagaacgac aacaaacctc tctctccgga
    61921 aacctggcag catctgaaag gcctggtgac caggcagctt tccggcaaac gtctgttcgt
    61981 tgtcgacgct ttctgtggtg cgaacccgga tactcgtctt tccgtccgtt tcatcaccga
    62041 agtggcctgg caggcgcatt ttgtcaaaaa catgtttatt cgcccgagcg atgaagagct
    62101 ggcaggtttc aaactagact ttatcgttat gaacggcgcg aagtgcacta acccgcagtg
    62161 gaaagaacag ggtctgaact ccgaaaactt cgtggcgttt aacctgaccg agcgcatgca
    62221 gctgattggc ggcacctggt acggcggcga aatgaagaaa gggatgttct cgatgatgaa
    62281 ctacctgctg ccgctgaaag gtatcgcttc tatgcactgc tccgccaacg ttggcgagaa
    62341 aggcgatgtt gcggtgttct tcggcctttc cggcaccggt aaaaccaccc tttccaccga
    62401 cccgaaacgc cgcctgattg gcgatgacga acacggctgg gacgatgacg gcgtgtttaa
    62461 tttcgaaggc ggctgctacg caaaaactat caagctgtcg aaagaagcgg aacctgaaat
    62521 ctacaacgct atccgtcgtg atgcgctgct ggaaaacgtc accgtgcgtg aagatggcac
    62581 tatcgacttt gatgatggtt caaaaaccga gaacacccgc gtttcttatc caatctatca
    62641 catcgataac atcgttaagc cggtttccaa agcgggccac gcgactaagg ttatcttcct
    62701 gactgctgat gctttcggcg tgttgccgcc ggtttctcgc ctgactgccg atcaaaccca
    62761 gtatcacttc ctctctggct tcaccgccaa actggccggt actgagcgtg gcatcaccga
    62821 accgacgcca accttctccg cttgcttcgg cgcggcgttc ctgtcgctgc acccgactca
    62881 gtacgcagaa gtgctggtga aacgtatgca ggcggcgggc gcgcaggctt atctggttaa
    62941 cactggctgg aacggcactg gcaaacgtat ctcgattaaa gatacccgcg ccattatcga
    63001 cgccatcctc aacggttcgc tggataatgc agaaaccttc actctgccga tgtttaacct
    63061 ggcgatccca accgaactgc cgggcgtaga cacgaagatt ctcgatccgc gtaacaccta
    63121 cgcttctccg gaacagtggc aggaaaaagc cgaaaccctg gcgaaactgt ttatcgacaa
    63181 cttcgataaa tacaccgaca cccctgcggg tgccgcgctg gtagcggctg gtccgaaact
    63241 gtaatgattt gaagctggag aatatctatc cagtatctta tagaaagcaa aacgggaggc
    63301 accttcgcct cccgtttatt tacccttctt ttgtcgtgcc ctgcgcccgc gttaccggca
    63361 ctggcagcca ggcgcgaatg gaaagcccgc cccgctcgct ggtgccaagc tccagcatcc
    63421 cgttatggtt atccacgata cgctgcacaa ttgccagccc taatcccgtg ccgctaatgg
    63481 tgcgcgcact gtcgccgcgg acaaacggct ggaacaggtg cttacgttgt tccggcgcaa
    63541 ttcccggacc gtcatcttcc acctggaacc aggcgcgatt cggctccgtt ccgctgctga
    63601 ctttgatcca gccattgcca taacgagcgg cgttgaccac catattcgcc accgcgcgtt
    63661 tgatcgacag cgggtgcatt ttcacttcaa tgctgccggg gtaaagcgcg gtttcaattt
    63721 cccgctcata gccactttcg gcagcaatca cctcaccgag cactgcatta agatccgcca
    63781 tttccatcgg catctcctgc ccggtgcgca ggtagtcgat aaactgctca atgatggcgt
    63841 tgcactcttc gatatcttta ttgatcgatt ctgccagata gccatcctgc tcgctcatca
    63901 tctcagtcgc caggcgaata cgcgtcagcg gcgtgcgcaa gtcgtgactt acccccgcca
    63961 tcagcagcgt gcggtcatcc gccagttgct taacaccagc cgccatatgg ttaaaggcgc
    64021 gggtaacgga acgcacctcg gacgcgccat actcacgcag cggcggcgga ataatccctt
    64081 taccgacctg caaagcagca tgttcgagat cgaccaacgg tcggttctgg atacgaataa
    64141 acagccacgc cccgcctatc gccaatagca taatcgccag cgtatagcgg aacagcggag
    64201 agaaatcgcc ctgatgaatt tcggtcagcg gcacgcgtac ccagatattg ggcgacagcc
    64261 aggttttcag ccagacgaca ggcgaacttt tgttgacctc aacgcgcact tccgtcgggc
    64321 cgcccagttg ctgtgccatc tgatggctta agaattcata gtgttgcgcc caacgcagac
    64381 ctgcctcttc ggcagcctcg ttggagtaga gagagatccc cagctcacgg tagatctccc
    64441 gacggaaagc gggaggcaca accaactgcg tgccgtcctc cagttgcagt ttgtcggtca
    64501 tcaacatacg cacttcgtac gcgaggactt tattaaactg ctggaggctc ggcaaaatcg
    64561 cgaagttcag caccaccaga taagtcgtca ccaggctggc gaacagcaag gtgacgatga
    64621 gcaataacgt acgggcaaat gaacttcgtg gcgagaagcg caatcgcctc atgctttaga
    64681 gccgtccggt acaaagacgt agcccaggcc ccaaacggtc tgaatgtaac gcggatgcgc
    64741 tggatcttct tctaccatgc ggcgcagacg cgaaatctgc acgtcgatgg agcgttccat
    64801 tgcggaatat tcacgaccac gggcaaggtt catcagctta tcgcgggaga gcggctcacg
    64861 cggatggctg accagtgcct tcagtaccgc aaactcaccg ctggtgagcg gcatcggctc
    64921 atcttcgcgg aacatttcgc gcgtaccgag gttaagtttg aacttaccga aagcaattac
    64981 cgcctcttcc tgtgacggtg cgcctggcag ttcgttcgcc tgacgacgca gcaccgcacg
    65041 aatacgggcc agcagttcac gcgggttaaa cggttttgga atgtagtcgt cagcgccaat
    65101 ctccaggcct acgatacggt ccacttcttc ccctttcgcc gtcaccataa tgatcggcat
    65161 cgggttgctc tgactacgaa gacgtcggca aatcgacaag ccatcttcac caggtaacat
    65221 taaatccagt accataagat ggaaagattc acgagtcagc aggcgatcca tctgttctgc
    65281 attagcgacg cttcgaacct ggaagccttg ttcggtgaga taacgttcca gcagcgcacg
    65341 caggcgcatg tcgtcatcga ccaccagaat cttgtagttc tcttgcattg tttgtactcc
    65401 caaaggttcg caacaatttg taagcgtgta ttcttaaaaa agctcacgtt cgtcaccagc
    65461 taaatctggt atgaatttca gcctaaattg ttacaaagca tattaaacag cagcttaagt
    65521 atacaattta ttcggcgaaa cattattgat tctgttgata tgatcacgtt atacccaatg
    65581 tgcgcattat caaacagaca aagggaatca acgagatgaa aacgcccctg gttacccggg
    65641 aagggtatga aaaactcaaa caagagctta attatctctg gcgtgaagaa cgcccggagg
    65701 tcacaaaaaa ggtgacctgg gccgcaagtc tgggcgaccg cagcgaaaat gctgactatc
    65761 agtataataa aaagcgtctg cgtgaaatcg accgtcgcgt gcgctatctc actaaatgca
    65821 tggaaaatct caaaatcgtc aattactccc ctcagcagga aggcaaagtc ttttttggcg
    65881 cgtgggtgga gattgaaaac gacgatggcg tgactcaccg tttccgtatt gtcggctacg
    65941 atgaaatttt tggccgtaaa gattacatct ctatcgattc cccgatggcc cgcgcattgc
    66001 tgaaaaaaga agtcggcgat ctggcggtgg tgaatacccc tgccggggaa gcgagctggt
    66061 atgttaatgc tatcgaatac gtgaaaccgt aatgaagagt cttaacctcc tgccgatggc
    66121 tggcattttt gccagccagt ccgtataact atcccctgat ttttgatccg aaaagatgaa
    66181 ctcaaaccat gatgaatgat tcgttctgcc gcattattgc gggtgaaatt caggcgcgcc
    66241 cggaacaggt tgacgctgcc gttcgcctgc ttgacgaagg gaataccgtg ccgtttatcg
    66301 cacgttatcg taaggaaatc accggcggtc tggatgacac gcagctgcgt aatctggaaa
    66361 cgcgtctgag ctatctgcgc gagctggaag agagacgtca ggcgatcctc aagtccattt
    66421 ccgagcaagg caaactcacc gatgatctgg cgaaggccat caacgccacc ctaagcaaaa
    66481 ccgaactcga agacctctac ctgccctaca aacctaaacg ccgcacccgc gggcaaatcg
    66541 ccattgaagc agggcttgag ccgttggctg acctgctgtg gagcgatccg tcacacacac
    66601 cagaagtcgc cgctgcgcaa tatattgatg ccgataaagg cgtggcagat accaaagccg
    66661 cgctggacgg cgcgcgctat atcctgatgg aacggtttgc cgaagatgcc gcgctgctgg
    66721 cgaaagtgcg tgattatctg tggaagaacg cgcatttggt ttctacggtg gtgagcggta
    66781 aagaagagga aggggcgaaa ttccgcgact atttcgatca tcacgaaccg ttgtccacgg
    66841 tgccttctca ccgcgcgctg gcgatgttcc gtgggcgtaa cgaaggcgta ctccagcttt
    66901 cgctgaatgc cgatccgcag ttcgatgagc cgcccaaaga gagctattgc gagcaaatca
    66961 tcatggatca ccttggcctg cgcctgaaca atgccccggc ggatagctgg cgcaaaggcg
    67021 tagtgagctg gacctggcgc atcaaggtgc tgatgcatct ggaaaccgaa ctgatgggta
    67081 ccgtgcgcga acgcgcggaa gatgaagcaa tcaacgtctt tgcccgtaac ctgcacgatc
    67141 tgctgatggc ggcccctgcc ggactgcgtg caacgatggg cctcgatccg ggtctgcgta
    67201 ccggggtaaa agtggcagtg gtcgatgcca ctggcaaact ggtggcgacc gataccattt
    67261 acccgcacac cggacaagcc gcaaaagtag cgatgaccgt tgctgccttg tgtgaaaaac
    67321 ataacgttga actggtggcg atcggcaacg gtacagcttc ccgcgaaact gaacgtttct
    67381 atctcgacgt gcagaagcag ttcccgaaag tgaccgcaca gaaagtgatc gtcagcgaag
    67441 ctggcgcgtc ggtttactca gcttccgagc tggcagcgca ggagttcccg gatctcgacg
    67501 tttcgctgcg tggtgcggta tctatcgccc gccgtttgca ggatccgctg gcggagctgg
    67561 tgaaaatcga tccgaaatct atcggcgtag gtcagtatca gcatgacgtc agccagacgc
    67621 aactggcccg caaactggat gcagtagtag aagactgcgt aaacgccgtt ggcgtcgatc
    67681 tcaacaccgc ttctgttccg ctattaactc gcgtggcggg cctgacgcgc atgatggcgc
    67741 aaaacatcgt ggcctggcgc gatgagaacg gccagttcaa gaaccgtcag caactgctga
    67801 aagtcagccg tctggggccg aaagccttcg agcagtgcgc gggcttcctg cgcattaacc
    67861 acggtgataa cccgctggac gcctctaccg ttcacccgga agcctatccg gtggtggaac
    67921 gcattctggc agcaacacag caggcactga aagatctgat gggtaacagc agcgaactgc
    67981 gtaacctgaa agcgtctgac tttactgatg aaaaattcgg tgtgccgaca gtaactgaca
    68041 tcatcaaaga gctggaaaaa ccgggtcgcg atccgcgtcc ggaatttaaa accgctcagt
    68101 ttgccgatgg cgtcgagaca atgaacgacc tgcaaccggg tatgatcctc gaaggcgctg
    68161 tcaccaacgt caccaacttt ggcgcgtttg tcgatattgg cgtgcatcag gacggcctgg
    68221 ttcacatctc ttcattgtcg aacaagtttg tggaagatcc gcataccgtg gtgaaagcgg
    68281 gcgacattgt gaaggtgaaa gtgctggaag tggatcttca gcgtaaacgt atcgccctga
    68341 ccatgcgtct ggacgagcag cctggcgaaa ccaacgcccg tcgcggcggc ggtaatgaac
    68401 gcccgcaaaa caaccgcccg gcagccaaac cacgcggtcg tgaagcgcag cctgccggta
    68461 atagcgcgat gatggatgcg ctggcggcgg caatgggcaa aaaacgttaa acgcccggac
    68521 tggcccctaa acctgcgcga taaggcgttc acgccgcatc tggcaacgaa cgccttgcct
    68581 gacataaaag tgccggagaa tatctccggc ctttttattc cacaaccaaa ctcataatat
    68641 attccggcaa tatttatcac ttcattaaca actgaaacct taattaaaca ttagccagtc
    68701 cgggtaattc actattcgaa ttatattttc gctgcgatat aaccttgagc cacatcaaca
    68761 ttgagtcaga ttattattca aaccaacatt cgcacacatt ttaagtattg ctgatagaaa
    68821 ccattctcat tatcattgtg ttgttgatta tttaatctct cctttgttgg caaatcatct
    68881 ggtctcatgt cgctgtcaaa cgccccatga ggtagttatc cagttaatga gaaacaagta
    68941 ggcacctatg caatacactc cagatactgc gtggaaaatc actggctttt cccgtgaaat
    69001 cagcccggca tatcgccaaa aactgctttc tcttggcatg ttacctggct cctcttttaa
    69061 tgtggtgcgc gtcgctccac tcggcgaccc cattcatatc gaaacccgtc gtgtgagcct
    69121 ggtattacgc aaaaaagatc tggccttatt agaagtggaa gcggtttcct gttaatacgg
    69181 tgataacaac aatgaaaaaa ttaaccattg gcttaattgg taatccaaat tctggcaaga
    69241 caaccttatt taaccagctc actagcgcac gtcagcgtgt aggtaactgg gctggcgtta
    69301 ccgtcgaacg taaagaaggg caattctcca ccaccgatca tcaggtcacg ctggtggacc
    69361 tgcccggcac ctattctctg accaccatct catcgcagac ctcgctcgat gagcaaatcg
    69421 cctgtcacta cattttgagt ggcgacgccg acctgctgat taacgtggtg gatgcgtcta
    69481 accttgagcg taacctgtac ctgacgctac aactgctgga actcggcatt ccctgcatcg
    69541 tggcactgaa catgctcgac attgccgaga agcaaaatat tcgtattgaa attgatgctc
    69601 tgtcggcgcg tcttggctgc ccagtgatcc cgctggtttc aacccgtggt cgcggtattg
    69661 aagcgctcaa gctggcgatt gatcgctata aagctaacga gaatgtggaa ctggtgcatt
    69721 acgcacagcc gctactcaac gaagcagatt cactggcaaa agtgatgcct tccgacatcc
    69781 cgctgaaaca acgtcgctgg ctgggcctgc aaatgctgga aggcgatatc tacagccgcg
    69841 cctacgccgg tgaagcgtcg cagcatctgg atgccgccct tgcccgtctg cgtaatgaaa
    69901 tggatgatcc ggcgctgcac attgccgatg cgcgttacca gtgcatagct gccatctgtg
    69961 atgtggtaag caacaccctg acggcagaac ccagccgttt caccactgcg gtagataaaa
    70021 tcgtgctcaa ccgtttcctc ggtctgccga ttttcctctt tgtgatgtac ctgatgttcc
    70081 tgctggctat caacatcggc ggggcgttac agccgctgtt tgacgtcggc tccgtggcgc
    70141 tatttgtgca tggtattcaa tggattggct acacgctcca cttcccggac tggctgacta
    70201 tcttcctcgc ccagggcctg ggtggcggta ttaacaccgt gctgccactg gtgccgcaga
    70261 tcggcatgat gtacctgttc ctctccttcc ttgaggactc cggctatatg gcgcgtgcag
    70321 cgtttgtgat ggaccgcctg atgcaggcgc tgggcttgcc ggggaaatcc tttgtgccgc
    70381 tgatcgtcgg tttcggttgt aacgtaccgt cggtaatggg cgcacgtacg cttgatgcac
    70441 cgcgtgaacg tctgatgacc atcatgatgg caccgtttat gtcctgcggc gcgcgtctgg
    70501 ctatcttcgc agtattcgcg gctgccttct tcgggcagaa cggtgccctg gcggttttct
    70561 cgctgtatat gctcggtatc gtgatggcgg tgctgactgg cctgatgctc aagtacacca
    70621 tcatgcgcgg tgaagcgacg ccgtttgtca tggagctgcc ggtctaccat gtaccacacg
    70681 ttaaaagcct gattatccag acctggcagc gtctgaaggg cttcgttctg cgtgctggta
    70741 aagtgattat catcgtcagc attttcctga gcgctttcaa cagcttctcg ttgagcggga
    70801 aaatcgtcga taacatcaac gactcggcgc tggcgtccgt cagccgggtg ataacccctg
    70861 tcttcaagcc gattggcgta catgaagata actggcaggc aacggttggc ctgtttacgg
    70921 gcgcaatggc aaaagaagtg gtggtgggta cgctcaacac cctctacacc gcagaaaata
    70981 ttcaggacga agagttcaat ccggcggaat tcaacctcgg tgaagagctg ttcagtgcgg
    71041 tagatgaaac ctggcagagc ctgaaagaca ccttcagcct tagcgtactg atgaacccca
    71101 ttgaagctag caaaggcgac ggtgaaatgg gtaccggggc gatgggcgtg atggatcaga
    71161 aattcggtag cgcagctgcc gcatacagct acctgatttt cgtcctgctg tatgtaccat
    71221 gtatctcggt gatgggagcc atcgcccgtg aatcaagccg tggctggatg ggcttctcca
    71281 tcctgtgggg gctgaatatc gcttactcac tggcaacatt gttctatcag gtcgccagct
    71341 acagtcagca cccaacttac agcctggtgt gcattctggc ggtaatcctg tttaacatcg
    71401 tagttatcgg tctgctgcgc cgcgcgcgta gccgggtgga tatcgaactg ctggcaaccc
    71461 gcaagtcggt aagcagttgc tgcgcagcca gcaccaccgg tgattgccat taatggcttc
    71521 acttattcag gtacgtgatt tgctggcgtt acggggccgt atggaagcga cccagataag
    71581 ccagacattg aacactccgc agccaatgat taacgccatg ctgcaacaac tggaaagtat
    71641 gggcaaagcc gtgcggattc aggaagaacc tgacggctgc ctctctggca gttgtaaaag
    71701 ctgcccggaa ggaaaagcct gtctgcgcga gtggtgggcg ctgcgttaac cttactccat
    71761 cgcctgtttt ggaaagcccg gtatgcgtct gcatccgggc ttttttgcgt gcggctttcc
    71821 ataaaaatgc aactcttgca gcacggcgta agttcctttg aaagcatctc gcagggatga
    71881 aaactcgcta atgcacaggt gtggagtggc gcgtagagtc gcggcattca aacaacaggt
    71941 gaaggaacgc catgagcaaa aagcagagtt ccaccccaca cgatgcgctg ttcaaactct
    72001 ttttacgcca accggacacg gctcgtgatt ttcttgcgtt tcatttaccg gcacccattc
    72061 acgcgctttg tgatatgaaa accctcaagc tggagtcgag cagctttatt gatgacgatc
    72121 tgcgtgaaag ctattccgat gtgctgtggt cggtgaaaac ggaacaagga ccaggataca
    72181 tctattgtct gattgaacat caaagcacct caaacaaact gatcgcattt cgcatgatgc
    72241 gttacgctat tgccgcaatg caaaatcacc ttgatgctgg atacaaaacg ttgccgatgg
    72301 tggtgctatt gttgttttac cacggtattg aaagccccta tccctattcg ctgtgttggc
    72361 tggattgttt cgccgatccc aaactggcaa ggcagcttta tgcctccgca tttccgctga
    72421 ttgatgtcac cgtcatgcct gatgatgaaa tcatgcagca ccgacgtatg gcgctgctgg
    72481 agttaattca aaaacatatt cgtcaacgcg
//
LOCUS       DAZPEP010000006        67235 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZPEP010000006 DAZPEP010000000
VERSION     DAZPEP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.006"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            50..682
                     /locus_tag="ACHUT9_000374"
                     /pseudo
     CDS             50..682
                     /locus_tag="ACHUT9_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            785..1287
                     /locus_tag="ACHUT9_000375"
                     /pseudo
     CDS             785..1287
                     /locus_tag="ACHUT9_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1354..2811)
                     /gene="nuoN"
                     /locus_tag="ACHUT9_000376"
     CDS             complement(1354..2811)
                     /gene="nuoN"
                     /locus_tag="ACHUT9_000376"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFR1346948.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2818..4347)
                     /gene="nuoM"
                     /locus_tag="ACHUT9_000377"
     CDS             complement(2818..4347)
                     /gene="nuoM"
                     /locus_tag="ACHUT9_000377"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFR1346949.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4511..6352)
                     /gene="nuoL"
                     /locus_tag="ACHUT9_000378"
     CDS             complement(4511..6352)
                     /gene="nuoL"
                     /locus_tag="ACHUT9_000378"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFR1346950.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6349..6651)
                     /gene="nuoK"
                     /locus_tag="ACHUT9_000379"
     CDS             complement(6349..6651)
                     /gene="nuoK"
                     /locus_tag="ACHUT9_000379"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFR1346951.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6648..7202)
                     /gene="nuoJ"
                     /locus_tag="ACHUT9_000380"
     CDS             complement(6648..7202)
                     /gene="nuoJ"
                     /locus_tag="ACHUT9_000380"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFR1346952.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7214..7756)
                     /gene="nuoI"
                     /locus_tag="ACHUT9_000381"
     CDS             complement(7214..7756)
                     /gene="nuoI"
                     /locus_tag="ACHUT9_000381"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFR1346953.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7771..8748)
                     /gene="nuoH"
                     /locus_tag="ACHUT9_000382"
     CDS             complement(7771..8748)
                     /gene="nuoH"
                     /locus_tag="ACHUT9_000382"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFR1346954.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8745..11471)
                     /gene="nuoG"
                     /locus_tag="ACHUT9_000383"
     CDS             complement(8745..11471)
                     /gene="nuoG"
                     /locus_tag="ACHUT9_000383"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFR1346955.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11524..12861)
                     /gene="nuoF"
                     /locus_tag="ACHUT9_000384"
     CDS             complement(11524..12861)
                     /gene="nuoF"
                     /locus_tag="ACHUT9_000384"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFR1346956.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12858..13358)
                     /gene="nuoE"
                     /locus_tag="ACHUT9_000385"
     CDS             complement(12858..13358)
                     /gene="nuoE"
                     /locus_tag="ACHUT9_000385"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFR1346957.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13361..15163)
                     /gene="nuoC"
                     /locus_tag="ACHUT9_000386"
     CDS             complement(13361..15163)
                     /gene="nuoC"
                     /locus_tag="ACHUT9_000386"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFR1346958.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15257..15919)
                     /gene="nuoB"
                     /locus_tag="ACHUT9_000387"
     CDS             complement(15257..15919)
                     /gene="nuoB"
                     /locus_tag="ACHUT9_000387"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFR1346959.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15935..16378)
                     /gene="nuoA"
                     /locus_tag="ACHUT9_000388"
     CDS             complement(15935..16378)
                     /gene="nuoA"
                     /locus_tag="ACHUT9_000388"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFR1346960.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17007..17945)
                     /gene="lrhA"
                     /locus_tag="ACHUT9_000389"
     CDS             complement(17007..17945)
                     /gene="lrhA"
                     /locus_tag="ACHUT9_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFR1346961.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18865..20082
                     /gene="alaA"
                     /locus_tag="ACHUT9_000390"
     CDS             18865..20082
                     /gene="alaA"
                     /locus_tag="ACHUT9_000390"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFR1346962.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20166..20765
                     /gene="yfbR"
                     /locus_tag="ACHUT9_000391"
     CDS             20166..20765
                     /gene="yfbR"
                     /locus_tag="ACHUT9_000391"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFR1346963.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20824..22656)
                     /locus_tag="ACHUT9_000392"
     CDS             complement(20824..22656)
                     /locus_tag="ACHUT9_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFR1346964.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22743..23391)
                     /gene="hxpA"
                     /locus_tag="ACHUT9_000393"
                     /pseudo
     CDS             complement(22743..23391)
                     /gene="hxpA"
                     /locus_tag="ACHUT9_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(23402..23896)
                     /locus_tag="ACHUT9_000394"
     CDS             complement(23402..23896)
                     /locus_tag="ACHUT9_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFR1346965.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(23979..24434)
                     /gene="yfbV"
                     /locus_tag="ACHUT9_000395"
     CDS             complement(23979..24434)
                     /gene="yfbV"
                     /locus_tag="ACHUT9_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFR1346966.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24772..25974
                     /gene="ackA"
                     /locus_tag="ACHUT9_000396"
     CDS             24772..25974
                     /gene="ackA"
                     /locus_tag="ACHUT9_000396"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFR1346967.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26049..28193
                     /gene="pta"
                     /locus_tag="ACHUT9_000397"
     CDS             26049..28193
                     /gene="pta"
                     /locus_tag="ACHUT9_000397"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1346968.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28383..29903
                     /gene="yfcC"
                     /locus_tag="ACHUT9_000398"
     CDS             28383..29903
                     /gene="yfcC"
                     /locus_tag="ACHUT9_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFR1346969.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29936..30478)
                     /gene="yfcD"
                     /locus_tag="ACHUT9_000399"
     CDS             complement(29936..30478)
                     /gene="yfcD"
                     /locus_tag="ACHUT9_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFR1346970.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30536..31087)
                     /gene="yfcE"
                     /locus_tag="ACHUT9_000400"
     CDS             complement(30536..31087)
                     /gene="yfcE"
                     /locus_tag="ACHUT9_000400"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFR1346971.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31143..31787)
                     /gene="yfcF"
                     /locus_tag="ACHUT9_000401"
     CDS             complement(31143..31787)
                     /gene="yfcF"
                     /locus_tag="ACHUT9_000401"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFR1346972.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31923..32570
                     /gene="yfcG"
                     /locus_tag="ACHUT9_000402"
     CDS             31923..32570
                     /gene="yfcG"
                     /locus_tag="ACHUT9_000402"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFR1346973.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32627..32989
                     /gene="folX"
                     /locus_tag="ACHUT9_000403"
     CDS             32627..32989
                     /gene="folX"
                     /locus_tag="ACHUT9_000403"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFR1346974.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33010..33903
                     /locus_tag="ACHUT9_000404"
     CDS             33010..33903
                     /locus_tag="ACHUT9_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFR1346975.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33951..34841)
                     /gene="rpnB"
                     /locus_tag="ACHUT9_000405"
     CDS             complement(33951..34841)
                     /gene="rpnB"
                     /locus_tag="ACHUT9_000405"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFR1346976.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLESNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35038..35811)
                     /gene="hisP"
                     /locus_tag="ACHUT9_000406"
     CDS             complement(35038..35811)
                     /gene="hisP"
                     /locus_tag="ACHUT9_000406"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFR1346977.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35819..36535)
                     /locus_tag="ACHUT9_000407"
     CDS             complement(35819..36535)
                     /locus_tag="ACHUT9_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1346978.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36532..37218)
                     /gene="hisQ"
                     /locus_tag="ACHUT9_000408"
     CDS             complement(36532..37218)
                     /gene="hisQ"
                     /locus_tag="ACHUT9_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFR1346979.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37308..38090)
                     /gene="hisJ"
                     /locus_tag="ACHUT9_000409"
     CDS             complement(37308..38090)
                     /gene="hisJ"
                     /locus_tag="ACHUT9_000409"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFR1346980.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38311..39093)
                     /gene="argT"
                     /locus_tag="ACHUT9_000410"
     CDS             complement(38311..39093)
                     /gene="argT"
                     /locus_tag="ACHUT9_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFR1346981.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39359..39928)
                     /gene="ubiX"
                     /locus_tag="ACHUT9_000411"
     CDS             complement(39359..39928)
                     /gene="ubiX"
                     /locus_tag="ACHUT9_000411"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFR1346982.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40023..41540)
                     /gene="purF"
                     /locus_tag="ACHUT9_000412"
     CDS             complement(40023..41540)
                     /gene="purF"
                     /locus_tag="ACHUT9_000412"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFR1346983.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41577..42065)
                     /gene="cvpA"
                     /locus_tag="ACHUT9_000413"
     CDS             complement(41577..42065)
                     /gene="cvpA"
                     /locus_tag="ACHUT9_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFR1346984.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42324..42986)
                     /gene="dedD"
                     /locus_tag="ACHUT9_000414"
     CDS             complement(42324..42986)
                     /gene="dedD"
                     /locus_tag="ACHUT9_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFR1346985.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(42976..44244)
                     /gene="folC"
                     /locus_tag="ACHUT9_000415"
     CDS             complement(42976..44244)
                     /gene="folC"
                     /locus_tag="ACHUT9_000415"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFR1346986.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVSQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44314..45228)
                     /gene="accD"
                     /locus_tag="ACHUT9_000416"
     CDS             complement(44314..45228)
                     /gene="accD"
                     /locus_tag="ACHUT9_000416"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFR1346987.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45384..46043)
                     /locus_tag="ACHUT9_000417"
     CDS             complement(45384..46043)
                     /locus_tag="ACHUT9_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1346988.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46126..46938)
                     /gene="truA"
                     /locus_tag="ACHUT9_000418"
     CDS             complement(46126..46938)
                     /gene="truA"
                     /locus_tag="ACHUT9_000418"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFR1346989.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46938..47951)
                     /locus_tag="ACHUT9_000419"
     CDS             complement(46938..47951)
                     /locus_tag="ACHUT9_000419"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1346990.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48017..49153)
                     /gene="pdxB"
                     /locus_tag="ACHUT9_000420"
     CDS             complement(48017..49153)
                     /gene="pdxB"
                     /locus_tag="ACHUT9_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFR1346991.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49252..50247
                     /gene="flk"
                     /locus_tag="ACHUT9_000421"
     CDS             49252..50247
                     /gene="flk"
                     /locus_tag="ACHUT9_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFR1346992.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50244..51422)
                     /locus_tag="ACHUT9_000422"
     CDS             complement(50244..51422)
                     /locus_tag="ACHUT9_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1346993.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51697..52917)
                     /gene="fabB"
                     /locus_tag="ACHUT9_000423"
     CDS             complement(51697..52917)
                     /gene="fabB"
                     /locus_tag="ACHUT9_000423"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFR1346994.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53076..55082
                     /gene="mnmC"
                     /locus_tag="ACHUT9_000424"
     CDS             53076..55082
                     /gene="mnmC"
                     /locus_tag="ACHUT9_000424"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFR1346995.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55203..55481)
                     /locus_tag="ACHUT9_000425"
     CDS             complement(55203..55481)
                     /locus_tag="ACHUT9_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFR1346996.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(55515..56063)
                     /gene="epmC"
                     /locus_tag="ACHUT9_000426"
     CDS             complement(55515..56063)
                     /gene="epmC"
                     /locus_tag="ACHUT9_000426"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFR1346997.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56063..56872)
                     /locus_tag="ACHUT9_000427"
     CDS             complement(56063..56872)
                     /locus_tag="ACHUT9_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFR1346998.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56872..57696)
                     /gene="mepA"
                     /locus_tag="ACHUT9_000428"
     CDS             complement(56872..57696)
                     /gene="mepA"
                     /locus_tag="ACHUT9_000428"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFR1346999.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57700..58785)
                     /gene="aroC"
                     /locus_tag="ACHUT9_000429"
     CDS             complement(57700..58785)
                     /gene="aroC"
                     /locus_tag="ACHUT9_000429"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFR1347000.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58820..59752)
                     /gene="prmB"
                     /locus_tag="ACHUT9_000430"
     CDS             complement(58820..59752)
                     /gene="prmB"
                     /locus_tag="ACHUT9_000430"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1347001.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            59918..60469
                     /gene="smrB"
                     /locus_tag="ACHUT9_000431"
     CDS             59918..60469
                     /gene="smrB"
                     /locus_tag="ACHUT9_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFR1347002.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60591..61463)
                     /locus_tag="ACHUT9_000432"
     CDS             complement(60591..61463)
                     /locus_tag="ACHUT9_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFR1347003.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(61450..61974)
                     /locus_tag="ACHUT9_000433"
     CDS             complement(61450..61974)
                     /locus_tag="ACHUT9_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1347004.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(61971..62441)
                     /locus_tag="ACHUT9_000434"
     CDS             complement(61971..62441)
                     /locus_tag="ACHUT9_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1347005.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            complement(62438..62986)
                     /locus_tag="ACHUT9_000435"
     CDS             complement(62438..62986)
                     /locus_tag="ACHUT9_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1347006.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(62961..63712)
                     /locus_tag="ACHUT9_000436"
                     /pseudo
     CDS             complement(62961..63712)
                     /locus_tag="ACHUT9_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63732..66374)
                     /gene="yfcU"
                     /locus_tag="ACHUT9_000437"
                     /pseudo
     CDS             complement(63732..66374)
                     /gene="yfcU"
                     /locus_tag="ACHUT9_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66456..67019)
                     /locus_tag="ACHUT9_000438"
     CDS             complement(66456..67019)
                     /locus_tag="ACHUT9_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1347007.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16754 a        18208 c        16737 g        15536 t
ORIGIN      
        1 taactcatta taaataatga taaaataacg tcttttattg ataatttata tatggaaaaa
       61 aattcctttt gttatattgt ttctgatttc actccctctg attattttta ccaaccctgg
      121 gtcactgcat tatcgccaac gccccgacac gtcagtcctg agcaattaga aaaaacagtt
      181 cgttatctta cacaaactgt gcatccacgt aatgccgaca atattgataa tctgaatagg
      241 tccgtagagt atataaaaga gttctttgtc agtagcggtg ccagagttac cgcgcagagc
      301 gtccccgatt ggctattacg actctgcgcc tggtagccag gattaccctt acccgcaatg
      361 tcctggcttt atcccgatcg gggggacttt attgcctggt cggcagaata caggatatca
      421 acgccgttcg tcaggtaaaa acggcattgt tatcatcccg ggatttatct gtttattcta
      481 tgaatgcccc atggtttatt cccggtattg atttctctga ccacctgaat tattggcaac
      541 acgatattcc cgccgtaatg attactgaca ccgcttttta tcgtaataaa caataccact
      601 tgcccggtga tacagcagac agattgaatt atcagaaaat ggctcagatg gtgttataac
      661 cttgttacac aacagtaaat aataaaaatt tctcgaggcg ggtccagaaa cagcgacatg
      721 tacattttat tttccctcgt ctgttttaag atgcgaaaca taaaattcaa tggaggaaaa
      781 ataaatggga atgattggtt attttgcaga aattgactca gaaaagatta accaactgct
      841 tgagtctact gaaaaacctt tgatggataa tatacatgac acgctttcag gattacgccg
      901 actggatatt gataaacgct gggatttttt acactttggg ctgacaggca cccctgcttt
      961 tgatcccgcg aagaatgatc cgctgagtcg tgccgtgctt ggtgaacaca gcctcgaaga
     1021 tggcatagat ggcttcctgg gattgacctg gaatcaggag ttagccgcga caatcgaccg
     1081 actggaatca ctggatcgaa gtgagctgcg taaacagttt tccattaagc gactcaatga
     1141 aatggagatt tatccgggag tgaccttttc agaagagtta gaggggcaac tctttgcttc
     1201 gatcatgctg atatggaaaa gctgattagc gcgtatcgac gtatgctgcg acaagggaat
     1261 catgcgctga cggttattgt tgggtgatgg tgtcagggca ccaaaaaaaa ccgccgaaca
     1321 tgtcggcggc tttctgactt acaaactgat agcttacatc agcggcattg ctaaccgcac
     1381 aatgctaatc agcggttgtg gccatacacc cagcaccagt accaacagtg cagagatcag
     1441 caccacgata ccgcctgcgc tgtactgcca gtttgacggt gcatcgcgac ccggttgttc
     1501 cggggcgtgc aggtacaggc tcaccgccac gcgcaggtag tagtaaaggc cgcttgccga
     1561 accgacaacc acggcgccca ccagccacca caagtgtgcc tggacaccga ccgccagcac
     1621 gtagaactta ccaataaagc ccagcgtcat cggaataccg gccagagaca gcatcatcac
     1681 cgtcatcacc gccgcgagga tcgggcgatg ccagaacagt ccgcggtaag agtacaggga
     1741 atcagcatcc gggccacgat acgggctgga catcaggctg accacgccga acgcgccgag
     1801 gctgctaaac agataaccgg ccaggtaaac ccctaccgct tccatcgaca tctcgccagt
     1861 ttgcagcgca atcagcgcca ccagcagata gccgaggtga gagatagatg agtaaccgag
     1921 cagacgtttg atattggtct ggctcagcgc catcaggtta ccgaagatga tggaggcaaa
     1981 ggcgataatc gccagcacca cgcgaatcgc ttcgctgtca cccatcggtg cgtagaggaa
     2041 cagacgcatc accacaccga agatagcgat tttgctcgcc gtcgccagga aagtggaaac
     2101 cggcgcaggc gcgccctggt atacgtctgg cgtccacagg tggaacggca ccagagagag
     2161 tttgaagccg aggccaacaa tcatcaggcc gaaacctgcc agcaacagcg gctcgttgag
     2221 cataccgtcg ccaaggtttt tacccaacgc gacaaacgac aggtcgccag actgcgcata
     2281 caccagcgcc ataccaaaca gcaggaaaga agacgctgcg gcagaaagga tggtgtattt
     2341 gatactggct tccagtgaac gtttctggcg gaaagcgtaa ccgaccaggc caaacagcgg
     2401 caaagagatc agttcgatac cgaggaacag agacgccaga tggttggcat tcgccagcag
     2461 gatcccgccc agcgcggcaa ttaacaccaa caggtagaac tcgtccttgt tgtcgttata
     2521 gccttcaagc cacgggtagg cgaaagtaca ggtggcgagg ctcgccaaca ataccagccc
     2581 ggtgtaaagc atggcgaaac catcaacgcg catcagcggc gtaacgtcca tagcgcctgc
     2641 ctggccgaca aaccagagcg aaaccagcgc cgcgttaagc ccaataaccg agagcgtagc
     2701 gttgaggaaa tgattgcgtc gccacgcaat ggagagcatc acaaccacca ccgtcaagcc
     2761 gacgatcagc aacggtagca gtgcgatcag gttttgtgga gttattgtca tggcgattta
     2821 cggccttgta gtagtaacgg aattaacaaa ccactgctgg atattgccaa tcgcggagtg
     2881 cgaggtatcc agaatcggct gcggatagaa gccctgcagt accagcagca ccaccagcaa
     2941 caggatcata aacagctcac gcagcgacat ccctggcagt tcctggctgg caatctcgct
     3001 tttcgcttta ccgaagtaag cgcgatgcaa catcgccagc gaataaacag atgcaaagac
     3061 cagcccaaag gtagagataa cggtaatcac cgggacaacc tggaagctgc cgaacagaat
     3121 cataaattcg ccgacgaagt taccggtgcc aggcatccca agcgttgcca ccgcaaagaa
     3181 cagcgacagt gctggcagcc atttcatctt gctccacaga ccgcccatca tgcgcatgtc
     3241 gcgggtatgg atacgttcat aaagctgacc acaaagaata aacagacccg ccgccgacaa
     3301 gccgtgcgca atcatctgga ttaccgcgcc ctggtaggcc aactggctgc cggtgtagat
     3361 agcaatcagc acgaagccca tgtgggaaac cgaggtgtag gcgatcagac gtttgatatc
     3421 ggtctgggcg aaggccatcc acgcaccgta gaagatgccg ataacaccca gccacatagc
     3481 aattggcgcg aactctgccg acgcgttcgg gaacagcggc agggagaaac gcagcaaacc
     3541 gtaagcggca gttttcagca agatccccgc gaggtcaacg gaaccggcgg tcggagcctg
     3601 ggagtgcgca tccggcagcc agccatgcag cggaaccacc ggcattttga ctgcgaaggc
     3661 gatgaagaag cccagcatta acaggtattc cgcaccactg gacattggcg tattcagcag
     3721 ctcttcatag ttgaaggtcc agacgccggt cgcattgtag tgaacaaaaa ccagcgccag
     3781 gatggcaatc aacatcacca gaccactcgc ctgggtgtaa atgaagaact tggttgccgc
     3841 cgtgatacgc gttttaccgt cagaggcttt atgcccccac agtgcgatca ggaagtacat
     3901 cggcaccagc atcatttccc agaagaagaa gaacaggaac atgtcgatgg caaggaacac
     3961 gccgataacg ccgcccagga tccacatcag gttgaggtgg aagaagccct gatatttttc
     4021 gatctctttc cacgaacata gtaccgccag cacaccgagc agaccggtca gcacgaccat
     4081 cagcagcgac agcccgtcaa tggcgagatg aatagagata cccaaacgcg ggatccacgg
     4141 catgtcgaat tcagactgcc actgcggaat tccggcggat tgcgtcagtg aataaccgcc
     4201 ctgcaaccac agttgcagcg acagcgccag cgtcaatccc atggtgatca gcgcgatcca
     4261 gcgtggcacc ttgacgccaa agcgttcggt ctgccagcac aggaagccgc caataaaggg
     4321 aattaatatt agccagggta gtaacatggc gatctttatt ccttgtaaaa gtcccgtcag
     4381 gaccggattt tcaacgaatt ctcacgacag aaaattctct tcggactggg ggtagtgtcg
     4441 gatgcaccgc gaaggcggct tatccgacct acgttcttat atgcccaggg gcaatcccac
     4501 aatccttaac tcaacgcagt accatcaaca gtgccagcac cacgaccgca ccgatgctca
     4561 tggatgccac ataccagcgc agatagccgt tctcacttaa cagcagacct ttacctgcaa
     4621 agcgggaaag gacagccgga atgttcatca ttgagttcag cggatcgcgt ttcagcaacc
     4681 aggcaatacc caggaacggc ctgacgaaca ctttgtcgta cagccagtca aagccccagg
     4741 cgttgtacca ccaggtactc agcaggcggc ccggcgcact gttggcgatg gaggtcacca
     4801 gagtacgttt acccagccac agccaagctg ccagcagaat gccgacaatc gcgaccacgc
     4861 cagaggtaat ttccagggtc aacatgctgc cgtgcgccag ttccgtcgtt tgcggaagca
     4921 cgccctgcag cggcggtaca atcagtgcgc caacgaaggt ggaaaggatc agcagcacaa
     4981 tcagcggcag gctgtgagtt acccctttca cggcgtgagc gtgaatttgt tcttttccgt
     5041 ggaagacgat gaaaatcata cggaaggtgt agagcgaggt cataaacgca ccgaccagac
     5101 ctgccaccat cagattgata tgaccattcg ccatcgcacc cgcgaggatc tcatccttac
     5161 tgaagaagcc cgcagtgacc agcggcagtg ccgacagtgc tgcgccgccc accaggaagc
     5221 agagataaac cagcggaata gatttacgca gaccgcccat cttgaagatg ttctgttcgt
     5281 gatggcaggc cagaatgacg gaaccggatg ccaggaacag cagcgcttta aagaacgcgt
     5341 gagtcatcaa gtggaaaatc gccgcatccc atgcctgcac gccaagcgcg aggaacatgt
     5401 agccaatctg gctcatggta gagtaagcga gaacacgttt gatgtcggtc tgtaccagcg
     5461 cggcaaaacc ggccagcagc agcgtaaccg ccccgacaat gcccaccaga tgcagaattt
     5521 ccggcgtcat caggaacagg ccgtgggtac gggcgatcag gtagacaccc gcggttacca
     5581 tggttgcggc gtggatcagc gcggagacag gcgtcgggcc cgccatcgcg tcggcaagcc
     5641 atgtctgcaa cggcaactgc gcagatttac cgaccgcacc gcccagcagc atcagcgtcg
     5701 cccacatcag catgttattg ccgtcagcaa agtgcgctgg tgccagttcc accatttcgc
     5761 ggaagttcag ggtgcccagt tcgttgtaaa gaatgaacag tgcgaaagcg aggaaaacgt
     5821 cacccacacg ggtcacgacg aacgctttca ttgccgctgc gccattcttc ggatcggtgt
     5881 aatagaaacc gatcagcaga taggagcaca ggcccacgcc ttcccagccg aggtacatca
     5941 gcagcaggtt gtcggcaagc accagaacta ccatgctggc gatgaacagg ttggtgtagg
     6001 cgaagaagcg agagtagccc tcttcaccgc gcatgtacca ggaggcgtac atgtgaataa
     6061 ggaaacccac accagtgacc accgagagca tggtcagcga caggccgtcc agcaccaggt
     6121 taaaaccgat gttaaagtcg cctaccgaca tccacgtcca cagcggctgg ctgtatgcct
     6181 gctcgccgtt agcgaagaaa tcaacgccga taaaggcggt taccagcgcc gccaggccca
     6241 cagagccaac gccgacgatc gccgaaacgt tttcagacca gcgcccacgg gagaacgcca
     6301 gcaggacgaa gccaatcaat ggcaaaataa tggttaaggc aagcatgttc atccgcgcat
     6361 ctcacttact gaatcgatgt tcaggttctg gcgacgacgg tgaagttgca gcagcagcgc
     6421 aaggccgata ctcgcttctg ccgccgcgag gctgatggcg agaatgtaca tcacctgacc
     6481 gtcggtctga ccccagtagc ttccggcgac aacaaaggcc agcgcggagg cgttaatcat
     6541 gatttccaga ccaatcagca taaacagcag attgcgacgg ataaccagac cggttaagcc
     6601 aagaatgaat aagattgccg cgaggatcag tccatgttgt aaggggatca tgcgtgctcc
     6661 tccgtttttc ttttcgcgct gtcgtcttta cgattgctca gcacttcacc cgcacgctct
     6721 tcacgaccga cgtggaaggc cacaaccaga cctgcgagca gcagcataga agccagttcc
     6781 accgccagta cgtaaggccc gaacagcgta atacccactg ctttagcact gattggcgta
     6841 ccgtcgatac cctgatcgtt aacaccgagg atggcgtaaa caatcaccac cagcatgatg
     6901 gccgacaaaa ttgccggacc aatccacacc tgcggtttca gccactggcg ttcctgttcg
     6961 atttctgaac cgcccaggtt gagcatcatc accacgaaca cgaacagcac cataatggca
     7021 cccgcgtaga cgataatttc cagcgcaccg gcgaagtaag cgcccagtga gaagaacacc
     7081 ccggaaatcg ccagcagcga aataatcagg tacagcagtg cgtgtaccgg attggtatgg
     7141 gtgatcactc gcaaggtcgc aagtatggct atcaggccac agatataaaa agcgaactcc
     7201 attgcccctc tccttacggt aacaggctct tgacgtcgat aggcttggct tcgttctctg
     7261 cttcgccctt atctttgccg tcgattgcca tacctgccat ccggtagaag ttatattccg
     7321 ggtatttgcc cggaccggag atcagcagat cctctttttc gtaaaccaga tcctggcgct
     7381 tgtattcccc catttcgaaa tccggggtta actgaatcgc cgtggtcgga caggcttctt
     7441 cgcacagacc acagaaaatg cagcgtgaga agttgatgcg gaaaaattcc ggataccagc
     7501 gaccgtcttt ggtttctgct ttttgcagcg agatacagcc gaccgggcag gctaccgcgc
     7561 agaggttaca ggctacgcaa cgctcttcgc cgtccgggtc gcgggtcaga acgatacgac
     7621 cacgataacg gggcggcaga tagatcggct cttccgggta cattcgcgtt tcgcgtttgg
     7681 cgaacgcgtg caggccgatc atccagatac tacgaacctg ggtgccgaaa cctactaaca
     7741 attcttttaa ggtcatggtc ttattgcccc ttattgcgcc tgccagagaa tgacagccgc
     7801 cgttaccagc aagttgatca gcgtcagcgg caggcagatt ttccagccga aggacattac
     7861 ctggtcataa cgcggacgcg gtaacgacgc acgaatcaaa atgaacatca tcataaagaa
     7921 cgcggttttc agcgcgaacc agatgaatgg cggtaacaac gggccttgcc agccaccgaa
     7981 gaacagcgtc accatcaatg cagagatggt cacaatcccg atgtactcac ccacgaagaa
     8041 cagaccgaac ttcataccgg aatattcaat gtggtaacca tccgccagtt cctgctcggc
     8101 ttccggctgg tcaaacgggt gacggtgaca taccgccacg cccgcgatgg caaaggtaat
     8161 aaaaccaaag aattgcggga taacgttcca cacatgcgcc tggctgttga cgatgtcggt
     8221 catgttgaat gaaccggcct gcgccaccac gcccatcagg gaaagcccga ggaacacttc
     8281 gtagctcagg gtctgcgcag aagcacgcat cgcacccagc aacgagtatt tgttgttact
     8341 tgaccagccc gcaaacagca ccgcgtaaac cgccagacct gccatcatca ggaagaacaa
     8401 aatcccgatg ttcaggtcgg caaccaccca acccggactg actggcacaa tcgcaaaggc
     8461 cagcagcagc gaggtaaagg caatcatcgg tgccagggta aagatgacgc gatccgagaa
     8521 tttcgggatc cagtcttctt taaagaacat tttgatcatg tccgcaacca gctggagcga
     8581 accgccccag ccaacacggt taggtccgta acggttctgg aacagaccca gcaggcgacg
     8641 ttcgccaaag ctcatgaatg ccccgcaggt gacaaccacc agcaggatca ccaccgcttt
     8701 gaggatggtc agcaggatct caatcagttc cggtgatatc caactcattg ttgtgcctcc
     8761 ttgagatcct caagatgcgc gccagccagc accggagcaa taccggacat acccatcggc
     8821 aagcccacct gccctgccgt cagcccttca gcgatttcaa ccggcagcgt gaccgtattg
     8881 ccatcgtaac taaaggagac gtgtgtacct gcgttcacac ctaacttcgc ggcatccgct
     8941 gggttgagtt tgatgtacgg ctgcggcata cggctctgga agaccggagc acgctgtgac
     9001 aattcatcgc tgccaaacag gtggtaatac ggcgcgatac gccatttccc gtcctgcggc
     9061 tggaagcgtg ccggtacgct ggtgaagtaa tccagaccat tttcgctggt ttcaaacaga
     9121 cgcacgcccg gatcgccaaa gcgcagtttg ccgcccactt cgtcctggaa tttgttccac
     9181 gcctgcgggg agttccagcc cggcgcccag gcaaacggca cttgcgaacg gtgcgcagtc
     9241 ggctggttgt taccttccat cgagaaggtg aacatggtgt caatatcctg cggctgacgc
     9301 ggctcatgaa cgctgatatt ggcgcgcatg gcggtacgac cgctgtaacg gtgcggttca
     9361 cgggccagtt tctgcccacg aatacggaat gtcgcatccg gcgcagcatc tttgatacct
     9421 gccagttccg ggattttcgc cacaacagcg tcaatcacat ggtcgagctg cgtccagtcc
     9481 acttcacggc tcagcagggt gctgtgcagc gagtgtaacc agcgccagct ttccagcatg
     9541 acagttttgc tgtcgtaata agcaggatcg taaacctgga agaaacgttg ggcgcggcct
     9601 tcgttgttga tcaccgtacc gtcgctttca gcaaaactgg cagcagaaag taccagatgg
     9661 gcgttttcca taatcgctgt gcgttgatga tcaaccacca tcaccagcgg cgctttagcc
     9721 agcgcagcat tcacgcgggt agcggaagcg tgacgatgca gatcgttttc cagcaccacc
     9781 accgcgtcgg cgcgtccggt ttccagttcg gtcaacgctt cttcaagcga accgccaccc
     9841 ataatgccca gccccatgct gttgacggaa cgggcaatca tggtgatacc aacgtcagcg
     9901 ccgcgacctt tcagggcttt cgcgacgtta gccgcagcct gaatcacctc tatgctaccg
     9961 gcgttcgtcc cggagataat caacggtttc ttcgcacctg ccagtgcctg cacgatgacg
    10021 tcgattttgc tttgcagctc aggttcgata ccgtcaaccg ctggcgcaga gttatccagc
    10081 gcatgggcga tggcaaaacc taaacgcgcc tgatcttcaa ccggtgcgcg gtaagtccac
    10141 gccgcgatat catccagacg ggtgtcatca acgttggtaa caaacagcgg atgcttcgca
    10201 cgttgaccga tgttgaggat tgccgcaatc tgccagtcag ccactttctg tgctgccgcc
    10261 atttcgcgcg ctttaccttt cacagcctga cgcactgcca gcgcgacgcg cgcgccggtc
    10321 tgggtaacgt cttcgcccag caccagtacc gcatcgtaag attcgatttc gcgcagagcc
    10381 ggagtataaa tgccgccttc acgcagcact ttcagcgcca gttgcagacg ttcctgctca
    10441 ccgtgagcga taccggtgta gaagttttct tcgcccacca gttcacgcag cgcaaagttg
    10501 ctttccacgc tggcacgcgg agaaccaata ccgatcactt tcttcgactg acgcagaata
    10561 tctgccgcgc cctgcattgc ctgttcggcg ttgagggtaa tgaaatcatc gccacgacgc
    10621 tgtactggct gacgcggacg atccttcagg ttgacgtaac cgtaaccgaa acgaccacgg
    10681 tcgcagagga agtagtggtt taccgtaccg ttgtaacggt tttcgatacg acgcagttcg
    10741 ccgtaacgtt caccggggct gatgttacag ccgatggaac attgctggca gatgctcggc
    10801 gcaaactgca tatcccattt acggttgtaa cgctcggagt gcgttttgtc ggtaaatacg
    10861 ccggtcgggc aaatttcgac caggttaccg gaaaattcgc tttccagcgt gccgtcttcc
    10921 gggcgaccga agtagacgtt gtcgtgcgca ccgtaaacgc ccagatctgt accgtcagcg
    10981 taatctttgt agtaacgcac acagcggtag caggcgatgc agcggttcat ttcgtgagag
    11041 atgaatggcc ccaaatcctg attacggtgg gtacgtttgg tgaaacggta gcgacggaag
    11101 ctgtgtccgg tcatcacagt catatcctga agatggcagt taccgccttc ttcacatacc
    11161 ggacagtcgt gcgggtggtt ggtcattaac cactcgacca cactttcacg gaactgtttc
    11221 gcttcttcgt cgtcaatgga aataaaggtg ccatcggaag ccggtgtcat acaggacatc
    11281 accaggcgac cacgcgtgtc ttccgcgttt tggtattgct tcaccgcaca ctggcggcaa
    11341 gcaccgacgc ttcccagcgc cggatgccag caaaagtaag gaatatcaag gcccagagac
    11401 agacaagctt ccagcaggtt gtccgctccg ttgacctcgt attctttgcc gtctacatga
    11461 attgtagcca ttagcatgct tccagttttc tcggtcaggg accgagcgtt aatcgaaatt
    11521 cggttaccag cgctctttca gcaggttcgg ctgaatccca ttaatcaaat gggtattgct
    11581 gaacggctgt ttgattcccg cctcaaattc ttcgcggaaa tatttgatgg cgctctgtaa
    11641 cggctccact gcaccaggtg cgtgggcaca gaaagtttta cccgggccta agaatcgaca
    11701 cagttgctca agtgtttcga tatcgcccgg ctggccttcg ccacgctcca gcgcacgcag
    11761 aattttcacg ctccacggca gaccgtcgcg gcacggcgta caccagccgc aggactcacg
    11821 ggcgaaaaac tcttccaggt tacgcaccag cgacaccatg ttgatctcat ggtcaaccgc
    11881 catcgccagc gccgtaccca gacggctgcc cgctttaccg atactttcga attccatcgg
    11941 cagatcaagg tgcgcttcgg tcaggaagtc agtccccgcg ccgcctggct gccaggcttt
    12001 aaatttcaga ccatcacgca taccaccggc gtaatcttcg aggatctcgc gtgcggtggt
    12061 gccgaacggc agttcccaca gtcccggatt tttcacccga ccggagaagc ccatcagctt
    12121 ggtgccagca tctttacttt tcgagatgtt ctgataccac tccacgccgt tagcgaggat
    12181 cgccggaacg ttacacaggg tttcgacgtt gttgacacag gtcggtttgc cccatacgcc
    12241 ggaggttgcc gggaaggggg gtttcgagcg tgggttagca cgacgccctt ccagggagtt
    12301 gattaacgct gtttcttccc cgcagatgta gcgccctgcc ccggtatgga cgaacagttc
    12361 gaagtcgaaa cctgttccca taatgttttt gccaagcaag cccgcttcag tggcttcggc
    12421 aatggcgcgg cgcagattta ctgccgcttc gatatattcg ccacgcagga agatgtagcc
    12481 acggtaagct ttcagcgcaa acgcggagat gagcatacct tccaccagca ggtgcggcag
    12541 ttgctccatc aacaggcggt ctttataggt gcccggctcc atttcatcgg cgttacacag
    12601 caggtaacgg atgttcatgg attcgtcttt cggcatcagg ctccacttca ggccagtcga
    12661 aaagcccgct ccgccgcgcc ctttcagacc agcgtctttt acctgattaa cgatttcgtc
    12721 cggagacagc ccggtcagcg ccttacgcgc cccttcgtaa ccgtttttgc tgcggtattc
    12781 gtccagccac actggctgtt tgtcatcgcg cagacgccag gtcagcggat gcgtttcggg
    12841 agtacggata atgtttttca tttataccgc tccagcagtt cagggatcgc ttccggggtc
    12901 agatgcgcgt gagtgtcctc atcgatcatc atgtttggcc ctttatcaca gttccccagg
    12961 cagcaagttg gcagcagcgt aaagcggcca tcaaatgtcg tttgccctgg tttgatgttc
    13021 agctttttct cgagcgccgc ctgaataccc tgatacccgt tgatatgaca gaccacgctg
    13081 tcgcaataac ggatcacatg gcgaccaacc ggctggcgga agatctgact gtagaacgtt
    13141 gccacacctt cgacgtcgct tgccggaata cccagcacat cggcgatcgc gtggatcgca
    13201 ccatccggca cccagccacg ctgcttctga acgattttca gcgcttcaat ggacgccgca
    13261 cgcgggtctt cgtagtggtg catctcgtgc tcaatcgctt cacgctctgc cgcactcagc
    13321 tcaaaagcct cggtttgtgg ttgttgattc tcgtgcataa ttagcggtcc acatctgaca
    13381 taacaaaatc gatactgccc agataaacaa tcaggtcaga caccaggctg ccgcggatcg
    13441 ccgccggaat ttgctgcaaa tgcgcataac tcggcgtgcg gatacgagtg cgataactca
    13501 tggtgctacc gtcgctggtc aggtagtaac tgttgatccc cttggtcgcc tcaatcatct
    13561 ggaaagattc attggcaggc atcaccggcc cccacgacac ttgcaggaag tgggtgatca
    13621 gggtttcgat atgttgcagc gtgcgctctt tcggcggcgg cgtggtcagc gggtgatccg
    13681 ctttgaacgg gccttccggc atgttgttga ggcactgctc aagaatgcgc agactctggc
    13741 gcagctcttc cactttcagc attacgcggg tgtagcagtc agaaacacca ccaccaaccg
    13801 ggatttcaaa gtcgaagttt tcatagccag aataaggacg cgccttacgc acgtcgaagt
    13861 cgatcccggt agcacgcagg cccgcgccag tggtgcccca ctccagcgcc tctttcgcgc
    13921 cataggcggc aacgccctgg gaacgacctt tcagaatggt attttgcagc gccgctttct
    13981 cgtaagacgc cagacgtttc ggcatccagt cgaggaactc acgcagcagg cgatcccagc
    14041 cgcgcggcag gtcgtgcgct acgccgccaa tacggaacca cgccgggtgc atacggaaac
    14101 ccgtgatcgc ttccaccaga tcgtaaattt tctgacgatc ggtaaaggcg aagaacacgg
    14161 gcgtcattgc gccgacgtcc tgaataaagg tcgagatgta cagcaggtgg ctgttgatac
    14221 ggaacagttc ggagagcata acgcgaatga cgttaacgcg atccggcacg gtgatcccgg
    14281 ccagtttctc taccgccagc acgtaaggca tttcgttaac gcagccgccg aggtattcaa
    14341 tacggtcggt atacggaatg tagctgtgcc aggactggcg ctcgcccatt ttctccgcac
    14401 cacggtggtg gtagccgatg tctggtacgc agtcgacaat ctcttcgccg tcgagttgta
    14461 aaacgatacg gaaagccccg tgcgccgacg ggtggttcgg accgaggttg aggaacatga
    14521 agtcctcgtt ttcggtgccg cgcttcatcc cccactcttc cggtttgaag gtcagggctt
    14581 ccatctccag atcctgtttg gctttggtca gctcaaacgg cgagaattcg gtagcgcgcg
    14641 ccgggtaatc tttacgcagc gggtgacctt tccaggtttg cggcatcatg atgcggcgca
    14701 ggttcgggtg accgtcgaaa gtaatgccaa acagatccca ggtttcacgc tcataccagt
    14761 tagcgttcgg gaacagtttg gtgaaggtcg gtacgtgcag gtcgttttct gccagcgcca
    14821 ccttcagcat gatgtcgcgg ttacgatcga tagaaatcag atggtagaaa acggaaaaat
    14881 ccgcggcagg taacccttcg cggtgtgtgc gcagacgttc gtccatgccg tgtaagtcaa
    14941 acagcatgac gtaaggtttc ggcagtttct ttaagaaatc gccaacttcc agtaattgtt
    15001 cacgcttgat ccacacaacg ggaaccccgg tgcgagtcgc ctgaacagta aaggcatccg
    15061 gcccaaaacg gttgcgcagt tcgccaatca ccggatcatc aagatgatca cgggtctgcc
    15121 aggcgggttc ttgcgcggtt aagtcggtca tattgttcac cattgcaaat ggtccgtggt
    15181 gactgtcggc gcagtaagct tcgcgaaata gatttaggaa taagcgaaga aaaatccctt
    15241 tgccgacagg cgcaaattaa atctcgtcag gtgtacgcag gttggttacg gcaatgcgtt
    15301 caccgcgctt gcgttcgcgc tctgattgca tattggcgcg ataaacgccc tgatcgccaa
    15361 ccacccagga gagcggacga cgttctttgc cgatagattc ctgcaacagc atcagtgcct
    15421 gcatgtacgc ttcaggacgc ggcgggcagc ccgggatata cacatcaacc gggatgaatt
    15481 tatcgacgcc ctgcacaacg gaataaatat cgtacatacc accagagttg gcacaggcac
    15541 ccattgagat aacccatttt ggttccagca tctggtcata cagacgctga ataaccggtg
    15601 ccattttggt aaagcaggtt cctgcaacca ccatcaggtc agcctgacgc ggcgaagcac
    15661 gcaatacttc tgcgccaaaa cgcgccacgt catgcaccgc ggtaaacgaa gtcaccatct
    15721 caacgtaaca gcaggaaaga ccgaagttat acggccaaat tgagttttta cgaccccagt
    15781 taaccatgtc attgagcttg cccataaaca cgtttttgtt aacttcttgc tccagagggt
    15841 cggttacgat ctcctgcttt tgcagggggt aacggtcgtt ctcaccgttg ggatctatgc
    15901 gggtgagcgt ataatccatc ttaatgcctc gcggttagcg ttgacgatta gcgatactgt
    15961 tcgtttccgg gttcatacgc tcgcggcgtg aacgcgcggg cgtccagtcc agcgcgccaa
    16021 tacgcaccag ataaaccaga cctgccagta acacaaaaat aaaaattgca gcttccacaa
    16081 agcctaccca gccgctttcg cggatagagg ttgaccatgc gaacagatac agcgcttcaa
    16141 cgtcgaagat aacgaagaac atggccacca gataaaactt ggcggacagg cgtaagcggg
    16201 cggagccgac cgagtcgata ccggattcaa acggcacgtt tttcgacctc gcgcgtgcgc
    16261 gaccgcctaa aaaccaaccg cctaccagca tcaggcaaca caggccaatg gcaacgataa
    16321 gaaagatagc gaatgcccag tgatgagcga tgacttcagt ggatgttgac atactcattg
    16381 cttactcatc aaaagtagcg ccagattcac tgctcttcac ggcagatgga cgccacatcg
    16441 attcatgggg aggaataaaa aaaaccttac aatcactgta gaaattcttt tatacagcta
    16501 attgatgtgg tcttttactc ctttctataa ccttttgtca actttaacaa aagtttcttc
    16561 acattagttt acataatatc aacaccatta gcatttaatg ccctttcacg ccagatcctt
    16621 gacgactcca ggataattag atgttgttga atcgtgtccg ttgtgaagca atggaaaaaa
    16681 tacgggtcta ttttgacagg aatttgtgtc gattcctccc cccaaaagag agtattttct
    16741 tgatctgtga cacgcttttg tcattccata acaaaaacgc agcaacaaat ttacgtattt
    16801 ttaacatcat tgtagcaggt gatttttttc aggcgattat ttgtgcgttc gggacgtgaa
    16861 tctctggtga ttgaaaaatg aacagttttg tacgttctgc actatgcgag gaaggatttt
    16921 tactaaaaaa aagccgctgg ggtttaaaac acccccagcg gctcgttttt acactattgt
    16981 ctcaggaatt atctatcgtc cgtcgattac tcgatatccc tttcaatcaa caatgaatca
    17041 tccccttccg gagcagacat tgggctgtat tgccacggat tatggtagct ctccatcgcc
    17101 tgatagatca cctgggccag ctcattatta ctggacgggt catagcacag caaatattcg
    17161 gtatcaggta gcggcggtaa gccatctact ccactcagca cacgcaggtc cgggctcatc
    17221 atttcaatcg gccttgccgt cacgccaaga ccggctttca ctgccgcacg aacggccgga
    17281 agcgtcgagg cgacataagc cagtcgccat ggaatatctg ctttattaag cgtcgccagc
    17341 accatatcgc gaaacggact tggatcatcc agcaatacaa gagggatcgg ctcgcctttt
    17401 tgcaatacgt attccgctgc gcagtaccag tgtgttggcg aggtacgcag gttcaatgcc
    17461 ttaaacgctg aaggacgatg ggtggttacc atcaaatcca cttcctggga ttccagcatt
    17521 tctgccatat aggcattacg tttaacgcgg acatccagcg ctaatttcgg ataaaccgaa
    17581 ctcacgcgat ttaacaggaa aggtaagatc gtatcggcag attcatctga agcaccgata
    17641 gttaacacgc cctgaagatt actgaacatt aatgatgagc aggcctcatc attaaaacgc
    17701 agtattttcc tggcgtaacc aagaagttga atgccatgtt cagttaacag tttgttgcga
    17761 ccgtgacgag cgaacagttc tttcccaacg agttgttcca gacgctgcat ttgctgactt
    17821 acggcggact gagtacgaca cacagcggca gctgcggcag caaaagtgtt cagatcggca
    17881 acagcaacaa atgttctcag cagatcgagg tcgaggttaa ttatcggacg atttgcactt
    17941 atcatatatt atcacttact ggcggctcat actgagctgg ttaatgctgt gcacacacaa
    18001 acaagcaatt ccatttgtaa tgtgcctccc tggcagtttc atccggaacc cggacgaaag
    18061 taaaaatgca tatgagttgc actaaaaaag cgactcacat tgttccgtta taatgcctga
    18121 agtagatcac agaatatatc ttcagggatc gcatatctat taagttactc actcttttct
    18181 atttatgaca tgcgcgtgtt tgtataaatg taaatgtgag tccttgttcc actctcgtgc
    18241 agcatcgctg gtcatacgcg aacacgtaca aacagcaatg gtgaggcggc atcaagagca
    18301 gggatccgtc atttatgcga gcattttacc ccaaaaacct tttatttata agggtcattg
    18361 cgaattatct gatgcaaagt tatgttatgt taggcaaagt aatcttctat tattaataag
    18421 cacatcaaaa cctttttgaa tattaaataa taattaatta gcatcatcct cattcattaa
    18481 ttccgcttaa caatagtttc acaccttgcc cccattgcga cacctcggaa atcatcacgc
    18541 tgttaaactc tccgtaatgc ggaaatatca tcatcgcgtt tcattagtga attcttaaat
    18601 gaggcatttt cacacaatta tcttacagat aaaaaaacca gacttgcaat taagaatcag
    18661 aacaattcac tatataacat tgcatgcaaa gcatatacac ctcattattt tgtcattatt
    18721 aagttattaa cagcacaatc gagccttccc ctctggcaaa atcttattct gcagaccttc
    18781 aaaacaccgt cctgggggag tacattgttc taagctgact tccacggcag ggagtggcga
    18841 taacagcaaa aaaggtcaag attcatgtcc cccattgaaa aatccagcaa attagagaat
    18901 gtctgttatg acatccgtgg tccggtgctg aaagaagcaa aacgcctgga agaagaaggt
    18961 aacaaggtac tgaaactgaa catcggcaac ccagccccat tcggttttga cgcgccagat
    19021 gaaatcctcg ttgacgtgat acgcaacctg cctaccgctc aagggtattg cgattccaaa
    19081 gggctttact ccgcgcgtaa agccatcatg cagcactacc aggctcgtgg catgcgtgat
    19141 gttaccgtgg aagatattta catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg
    19201 caggcattgc tgaacagcgg ggacgaaatg ttggttcctg caccagatta cccactctgg
    19261 accgcggcgg tttcgctttc cagcggtaaa gcggtgcatt atctttgcga tgaatcctct
    19321 gactggttcc cggacctcga tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc
    19381 gttattatca acccaaataa cccaaccggc gcggtatatt ccaaagagct tttaatggag
    19441 attgtggaga ttgcacgtca gcataatctc attatcttcg ccgatgaaat ttatgacaaa
    19501 attctctacg acgatgctga gcatcactca attgcgccac tggcacctga cctgctgacc
    19561 attaccttta acggactgtc gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg
    19621 gtgttgaacg ggccgaaaaa acacgccaaa ggctacatcg aaggtctgga aatgctggct
    19681 tcaatgcgcc tgtgtgctaa cgttcctgcg caacacgcca ttcagaccgc gctgggtggt
    19741 tatcagagca tcagtgaatt tattacccct ggcggtcgtc tttatgagca gcgtaaccgc
    19801 gcgtgggaac tgatcaacga tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg
    19861 tatatgttcc cgaaaatcga cgccaaacgc tttaacattc acgacgatca gaaaatggtg
    19921 ctggatttcc tgttgcagga aaaagttctg ttggtgcaag ggacggcatt caactggccg
    19981 tggccggatc acttccgcat tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg
    20041 agcaagttcg cgcgtttcct ttctggttat catcagctgt aatcttaatt tcactgccgg
    20101 agattgcatc cggcagcgtt atcccgccac aatgacctga tgatgtcatc atacgtaagg
    20161 tcactatgaa acagagccat ttttttgccc atctctcccg cctgaaactc attaaccgct
    20221 ggccgctaat gcgcaacgtg cggacggaaa atgtgtccga acacagtttg caggtagcga
    20281 tggtcgccca tgcgctggca gctatcaaaa atcgaaaatt tggcggtaat gtcaacgccg
    20341 aacgtatcgc tttactggcg atgtaccacg atgcctcaga agtgctcacc ggcgatctcc
    20401 ctactccggt gaaatacttc aattcgcaaa tcgctcagga gtacaaggct attgaaaaaa
    20461 tcgctcagca aaaactggtc gatatggttc cggaagagct gcgggatatc tttgcgccgt
    20521 taattgacga gcatgcatat agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac
    20581 tgtgtgcata tctgaaatgt ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg
    20641 caaaaacgcg actggaagcg acgcttgaag cgcgtcgcag ccaggagatg gactacttca
    20701 tggaagtatt tgttcccagc ttccatcttt cgctcgatga gattagccag gattcaccgc
    20761 tgtaagcagc cggagtctgc gtcgcatcag gcaataagcg ccggatgcga catcaggctc
    20821 ttgtcaaaac ggaaacagca tcgggatcat caccacacaa accgccatca cgataatggt
    20881 gaacggtacc cccaacttca caaagtcact gaagctgtaa tttcccggac ctaaaaccag
    20941 tgtgttaaca ggtgaagaaa ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac
    21001 catcgcgaat ggataaggcg acacccccat cgttttggca gcagccagcg caatcggagc
    21061 catcaacacc gccgtcgcgg tattagagat aaatagccca ataaccgccg acaagacaaa
    21121 caaacacccc agcatcatat gtggcccgta accaccgcca atgtccatca gccctttcac
    21181 cgccagcgcg acacctcccg ttttctgtaa tgccacagca aacggcatca tcccaacgat
    21241 caaaataatg ctcggccagt gaatggattt ataggcgctt tcagcatcta tacagcggaa
    21301 tttccccatc agcaggcagg cgatgatagc ggcaacagga ttaggaattt catctgtcag
    21361 cattaacgcc accattagca ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg
    21421 cgatgcttca ctcacctctt ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc
    21481 cagcataccg atcagtttcc agttacccac aaccaggatg atatcgccca gcagcagagg
    21541 ctcatccgcc agcgaacctt ccagcgccac gccattgcgc tttagcccca ccacattcag
    21601 tccgtagcgg gtacgaaaac caatttcgcg caccgattta ccaatcagtt ctgactcagg
    21661 aattaatgaa atctctgcca tgcccacatc aagggcctgg tcagaaaaat actcgccgcg
    21721 cagtaccatc ggctccagca attgctcact acaaaattgc cggagatcga catcagccgc
    21781 agacatatca ataagcaaaa cgtcacgcgc gcgaaattca gaaaccccat taacgttcac
    21841 gataacgcga cgaaaacgcc gccagcgttc aacaccgatg acgttagcgc cataacgctc
    21901 acgtaatttg agatcatcca gccgttgacc aatcattggc gatccggggc gaatagccag
    21961 acgtcgcgca cgcccggtca gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt
    22021 ccagccttcg cgctgcgggg tctgggtatc ccctttcagc atgaaacgca tcactaacat
    22081 atacaaaata cccagcacca gcacgaccag gccaataggt gttacgctaa agaaactgaa
    22141 gccgtgatag ccttcacgca gcaattcact gttgactacc aggttcggcg gcgtcgccac
    22201 cagcgtcatc atgccgctta tcagcccggc aaaactcaac ggcatcatca gacgtgacgg
    22261 cgaagtttgc atacgcatgg caacgcttaa caccacggga ataaagatag cgacaacgcc
    22321 ggttgaactc ataaacgcgc caagccccgc gacggtcagc atcaacaaaa ccaacatttt
    22381 gatttcactg ttgcccgcaa ctttgaccag ccatgttccc attacggtgg caacaccggt
    22441 acggaccaaa ccatcgccaa taataaacaa ggcggcaatc aggacaacgt taggatcaga
    22501 aaagccggaa aatacttcgg ggactgtcag cgttccgctt aatgcaaacg cgacaataac
    22561 aaacaaagcg accgcatcca tacgcactct gcccgtcgca aacaagacaa tggcaacggc
    22621 cagtaatgaa agaacccaaa tcaattcacc gttcacaact tatccttgtt aattgaggga
    22681 gatgtcttga ttctgccata aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg
    22741 tttcactgaa taataacatc gccatttggc tgtttggtca cagtaatttg ctccagactg
    22801 tggaggacca aatcgacctc attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg
    22861 acatgacaac ccgccgccag gccagaaagc acgccagcgg agcatcttcc accaccacac
    22921 actcctgcgg cgcaagcccc agcagctgcg cgcctaacag atacgcatca ggttctggtt
    22981 ttccgcgctt cactcgctca gcggttacaa acacctctgg tgcgggaagc ccagctattt
    23041 tatggcgcgc tcgcgctacc ggcatggagc cagaagtcac aatggcccac ggaatacctg
    23101 ctttattcaa atgactgagt aaggcgattg cccccggaag cgggtaatac cttcggtttc
    23161 cgtggcctcg atgtgctcca gacgcgtaaa ctcggcggca atatcagcct cggatttgcc
    23221 cgccataaaa tggcgcagag aggtgatcgc ctgtttaccg tgaatgaaag ccagcacctc
    23281 ttccggcgct aacccatgac gtctggccca gttgctccac gcccgttcta ccgcaggcag
    23341 ggaatccacc agcgttccat caagatcaaa cagaaaacct ttgcaccgca cgcgggcctc
    23401 ctcaggcatt gataatttga ttaatttcgt tggcgctcaa atggtactga cgcgggcagg
    23461 catgccacac attaagcatg cgctgatatt tttcccacat tggcgtctgg gcgttaaaac
    23521 cgtgagttcc ggcgtcaaaa tgggtatagc gcccttccac attaaccata aagcggacat
    23581 aaccgaggta acgtgcttca gtggcggcgt caaagccgag gaaggtgaca cgacgttcat
    23641 cgatggattg ctggtcctgc aaattagacc aggaaacatg caacgcatga tacatctcca
    23701 taatgtcgat gatagtgcgg caggtttctt ctttcagctc gccaaactcg cgatccaatt
    23761 cacgcatctg taatccgtaa cctcgctcaa taattgtttg caggcgacgg taacgttcag
    23821 catttgccgg atcgagcata gtcatcatct tgtactgatt agacaaaata agacgttgcg
    23881 cgttggtcat ttccattgtt gactcctgta tcactctact acggtgaaaa aaaagaaggc
    23941 tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc aaggaaagtt
    24001 ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta atcaggcttg
    24061 ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac ttcataaaac
    24121 cagttaagga ttgcaggggg taatggcgtt acagaacgct tgcccagcca ccacaatccc
    24181 tgcatgggta aacttaaggc gaacagtgca gtggcaactg ccggcccaag ctgaccgccc
    24241 agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg gatcgcataa
    24301 cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt ttccagtggc
    24361 cacgtctttg agtaatgctg tccgcggcga aacaagctaa aaaaattaac agaacgatta
    24421 tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat ttgtgcaaat
    24481 tcacaactca gcgggacaac gttcaaaaca ttttgtcttc catacccact atcaggtatc
    24541 ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat tcatatacat
    24601 tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc ttccatcatg
    24661 caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca tgcgctacgc
    24721 tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc catgtcgagt
    24781 aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat cattgatgca
    24841 gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc tgaagcacgt
    24901 atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg cgccgctcac
    24961 agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga actgtctgcg
    25021 cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac cagctccgta
    25081 gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc accgctgcac
    25141 aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca gctgaaagac
    25201 aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga gtcttacctc
    25261 tacgccctgc cttacaacct gtacaaagag cacggcatcc gtcgttacgg cgcgcacggc
    25321 accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc ggtagaagaa
    25381 ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat ccgcaacggt
    25441 aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat gggtacccgt
    25501 tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg catgagcgtt
    25561 gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac cgaagtgacc
    25621 agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa gcgcgcaatg
    25681 gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct gatggatggt
    25741 cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat ggttcgtgaa
    25801 ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg caacctggct
    25861 gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc ggtggttatc
    25921 ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc ctgatttcac
    25981 accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt aacgaaagag
    26041 gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc ggtctgacca
    26101 gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg agcgttttca
    26161 aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg actatcgtgc
    26221 gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac gttgaaggtc
    26281 tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac taccacgcta
    26341 acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt aagcaccagt
    26401 ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc gtcttcgtta
    26461 tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg acccgcaaca
    26521 gcttcggcgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa ctgaacgcac
    26581 cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac gactcttcca
    26641 aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagccct ctgccggttc
    26701 tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat atggctcgcc
    26761 acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt aaatccgtca
    26821 ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt tctctgctgg
    26881 tgacttccgc agaccgtcct gacgtgctgg tggccgcttg cctggcagcc atgaacggcg
    26941 tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc atttctaaac
    27001 tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaacacc aacacctggc
    27061 agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat cacgaacgta
    27121 tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc gaatctctga
    27181 ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat cagctgactg
    27241 aacttgcgcg caaagcgggc aaacgtatcg tactgccgga aggtgacgaa ccgcgtaccg
    27301 ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg ctgggtaatc
    27361 cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actgggtgca gggattgaaa
    27421 tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa ctgcgtaaga
    27481 acaaaggcat gaccgaaacc gttgcccgcg aacagctgga agacaacgtg gtgctcggta
    27541 cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt cacactaccg
    27601 caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc tccctggtat
    27661 cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac tgtgcgatca
    27721 acccggatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct gattccgctg
    27781 cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt acttctggtg
    27841 caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa aaacgtcctg
    27901 acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac gttgcgaaat
    27961 ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gtttatcttc ccggatctga
    28021 acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc tccatcgggc
    28081 cgatgctgca gggtatgcgc aagccggtta acgacctgtc ccgtggcgca ctggttgacg
    28141 atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag taacctcgtc
    28201 atcatccgca gctttgcgct gcggatatct gaaccggaaa taatcactat ttccggtttt
    28261 ttattctctt aatctgcatt aatcctttct gattatcttg cttaactgcg ctgcatcaat
    28321 gaattgcgcc atttcacttt acatacttac cactttgttt tgtgcaaggg aatatttgcg
    28381 ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc gatacgttgg
    28441 tgattatctt ttttgttgct attttaacca gccttgccac ctgggtagtt ccggtgggga
    28501 tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca cgcaaagtcg
    28561 tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag tatcaccgcg
    28621 tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg tttgaaggat
    28681 taacctcagg atcgaaatac gggacagctg ttggcatcat catgtttatg ctggtgattg
    28741 gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc ctggcgctta
    28801 ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt ctgttttcac
    28861 ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt atcatcgcac
    28921 cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgacc tatattgcca
    28981 cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggtcgtt gctcagggga
    29041 ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg gttatcgcca
    29101 ctatgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag aatcctcttc
    29161 tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggcggat gttgaacaac
    29221 gtccgtttac ctttggtgac tggctggtat tgattgtcct gaccgccgta atggtctggg
    29281 tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc cagttcttca
    29341 ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc atgacggtta
    29401 ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct gccctgctgg
    29461 tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg tgaagcgggt gatgccagcg
    29521 tgttaaatac catcctcaac agcattgcca atgccattag cggtctggac aacgcggtcg
    29581 cggcctggtt tatgttgctc ttccaggcgg tatttaattt cttcgtgacg tccggttctg
    29641 gtcaggcggc gttaaccatg ccgttactgg caccgcttgg cgatctggtc ggtgttaacc
    29701 gtcaggttac cgtgctggca ttccagtttg gtgatggctt cagtcacatc atttacccaa
    29761 cctcagcttc gttaatggca actctcggtg tgtgcagggt ggacttccgt aactggctga
    29821 aggtgggcgc gaccctactt ggactgctgt ttattatgtc cagcgtcgta gtgatcggcg
    29881 ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc ttttttcatt
    29941 ctgccgtttc agtctctacg gcttcatttt tggcattgcg cttcatccac aacgccagcg
    30001 ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc ggcgtcagcc
    30061 agcaaacttc actgacttca tcttcctgta gggcgaaggg accgtgagag acgcagctga
    30121 acaacgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg tgctcggcaa
    30181 aggggacacc ggcaatgccc aactcttctt ccgcttcgcg acgcgcggat tccagcagtt
    30241 gctcatcggc ctgaaccaca ccgcctgcgg tcgcatctaa catgccgggt aaaaagtctt
    30301 ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg acgatgtaag
    30361 ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt gcaatgactt
    30421 cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc tgttccatta
    30481 tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgaattgtgg gtaaattacg
    30541 gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca ctaagtacgt
    30601 cattatccag catgccataa ctcgccggat taccgccttt cggaatactc accgagccgg
    30661 ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggta tgaccgtaca
    30721 ccagcacatc gttctggttt aaagcaggta gattttccgg gccaaaaaga tgaccatgcg
    30781 tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt atcgggaaat
    30841 gcagcagcat ttgatccact tcgctgtcgc agttgccgcg cacagcgata accttatgtg
    30901 ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt aaagcattac
    30961 gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg ctttgggcaa
    31021 acaactccag aacacgttcc gtcgccggta acgacccatg aatgtccgat gcaaacatca
    31081 gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt ctgtcgttgc
    31141 tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc tcgctgccac
    31201 tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag taccaggcgg
    31261 ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa attcggctga
    31321 cccagtgcta acaaatgttc tgccatcgcg aacagtttct ctgcactggc ttttccctcg
    31381 gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg ctcttcgcgg
    31441 atgggcatca gatcgctgcg cagccaggcc tgaatccgtc gtgcacgcgc acgattttct
    31501 aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc cagatactcc
    31561 gcaatggcag aagattcact caactcaaaa tcatcgattt gtaataacgg cacacggcgt
    31621 gtctgaccgt aaccttgcca cgtcggcttc aaatgttcac cgctgtcgag gtcgatggtc
    31681 ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aggcggataa cacataaggg
    31741 gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat gtcctcggca
    31801 ataaaggggg ctggctatca aactatagag cctttttgtc actgtcacgt gacgaatact
    31861 cacgtaagtc gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca accggagctg
    31921 caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg ctgtttctcg
    31981 aagaagcaga gcttgattat cgcttgatta aggtagacct ggggaaaggc ggtcagtttc
    32041 gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt gatcattctc
    32101 ctgccgatgg cggcgaaccg ctaagcctct ttgagtctgg tgccattttg ttgtatctgg
    32161 ctgagaaaac aggactcttt ttgagtcatg aaacgcgtga gcgcgccgcc acattacagt
    32221 ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat cattttaatc
    32281 acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa actcagcgtc
    32341 tttaccatgt actaaacaag cggctggaaa actcgccctg gctgggaggc gagaactaca
    32401 gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgccag cgaattgacc
    32461 tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc cctgccaccg
    32521 ggcaggcact gctaaaagca caactcggtg atgagcgttc ggatagttaa cagaaacagg
    32581 ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg cacaacctgc
    32641 cgctattatt cgtataaaga accttcgttt acgtacgttt atcggaatta aggaagaaga
    32701 aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg ccgataaagc
    32761 gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga aaaacattat
    32821 tcagcatgta gagaataacc gtttctcttt gttggaaaaa ttaactcagg atgtgctcga
    32881 tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata aactgcacgc
    32941 gctgcgctac gccgattcgg tatccatgac cttaagctgg cagcgttaac cgccatatcg
    33001 ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg ccatttgatt
    33061 ccgcgtttgc tggagctggg ccatcaaatc acggtagtga cgcgtaaccc acagaaagcc
    33121 agttccgttc tcggccctcg ggtgacgcta tggcaagggc tagccgatca aagcaacctc
    33181 aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa acgctggact
    33241 cacgagcaaa aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa actggtcgat
    33301 ttgattaatg ccagcgacac gccgccgtcg gtactcattt ccggctcggc aacgggctat
    33361 tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa cgaatttact
    33421 cataaactct gcgcccgctg ggaagagatt gcctgccggg cgcaaagtga caaaacgcga
    33481 gtgtgcctgc tgcgaaccgg cgtggtactg gcaccggacg gcggtattct cggtaaaatg
    33541 ctgccaccgt ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca gtatctggcc
    33601 tggattcata ttgatgatat ggtcaacggc attctctggc tgctggataa cgacctgcgc
    33661 gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc ccatgcgctc
    33721 ggtcaagcgc tgcatcgccc ggccattttg cgcgtccccg caaccgccat tcggctgtta
    33781 atgggcgaat cttcaatact ggtattaggt ggacaacgcg cgctgcctaa acggctggaa
    33841 gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga tgtcgttcgc
    33901 tgatgtggtt tacagcaaac atccgccagt taactcccgg tgttacagga ttagtggctt
    33961 tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca taactaactc tctgtctaaa
    34021 cctctatcca gcatctcctg agcaatacgc agggcttctt cgtgtttgcc ctgcattgcg
    34081 ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg tggtgcgcgt
    34141 tctgttatct caccaataaa tgcacggaaa cgctgggcat cccctgtttg taatacgtaa
    34201 ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt
    34261 tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc
    34321 gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga
    34381 aacgctgatg aatatatttt gcgggctata gcaggctcgg caaattcatc aagccagcag
    34441 agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat caccaatgga
    34501 agctctttat agcccgcatc aagatggttt tgcattgccg caatggaata acgcatcatg
    34561 cgaaaagcca ttaattcttc cagcttactt tggtgctcta tcactacata aatataaccc
    34621 actccctcct gcgttttcac agaccacaag aggtcggaat aatattgccg caggtcttca
    34681 tcaataaaac tgtttgattc tagtttaagc gtcgttaaat cacacagttt gcgcagcggc
    34741 gcgggaagat gaatatcaat aaaatcccgc gcggtgtctg gatggcgtaa aaaagattta
    34801 aataccgcat catgcggcgt ggaagttgtc gatattgtca tggcgttccg tcacccctca
    34861 aatttaagat gacgcgacaa taaccacgat taacacccaa tgcagcaggc agtttttatc
    34921 tttggacagc gaattcagaa gtatttttag cggactgcaa cttttacatt tcagcggaag
    34981 ctgactcgca aattccccat gaaaatactc cggcagcgtt agccaccgga gaggaaatta
    35041 tttcagcgat cccttaagga actgttgcag acgagggctt tgcgggttgc caaataactg
    35101 ctccggcgcg ccctcttctt ctattttccc ctgatggagg aaaatgacgt gagtagaaac
    35161 atggcgagca aagcccattt cgtgagtcac taccaccatg gttttcccct cttctgccag
    35221 ttgctgcata atacgtaaca cttcgcctac cagttcagga tcgagcgccg aggtaggttc
    35281 atcaaacagt aaaacttccg gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg
    35341 ctgaccgccg gaaagatgca ccggatattt cccctgcgca cgttcgtcta tcccgacttt
    35401 cgccagatac ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat
    35461 cggcgcttcc atgacgtttt ccagcaccgt catatggctc cagagattga agtgctggaa
    35521 taccatcgtc aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga ctttgagttg
    35581 accgtctttg tcgcgcacca gattgatcgt ctggccattg accacgatcg acccttcact
    35641 cggtttttcg aggaagttaa tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc
    35701 gatgatgctt attacatctc cggcattcgc ttgcagtgag acccctttca gcacttcatg
    35761 ttcgccgtag cgtttgtgca aatcgataac gtttaattta ttctcggaca tcgtattctc
    35821 agtgcgttga agaaggtttc acatgctgca accagcgttt ttccgctctg cgaaagaggc
    35881 tgatcaggac ataagagatg attaaataga gcaccgcggc aatgccgaag gcggtaaaag
    35941 gttgatacgt ggcggcgtta atatcgcggg ctattttcag cagatccggc acagtggccg
    36001 taaacgccag cgcagtagag tgcagcatca ggatcacttc gttgctgtat gccggtaacg
    36061 caatacgcag cgcagaaggc aaaataatgc aacgatacat tttaaaagtc gagaagccat
    36121 aggctctggc ggcttcaatt tccccatgcg gaaccgaacg gattgcccca gcaaaaatct
    36181 cggtagtgta agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc aggccactgc
    36241 ggaagaaagc gttaaggaat tcggttccct taacaatctc aagcgtgtac atgccggaat
    36301 agaacaccag caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga
    36361 ttggaaactg gatgtattta ttactggaga cacgaccaat cgccagaaac agcgccagga
    36421 ctccgcctat cactaccgac aaaataagca gccacagagt gatcgccaca ccagtaaagc
    36481 gataaccgtc ggtccacagc agcggtttcc agtattcatg taagatttcg atcacaggtc
    36541 agccctcttc acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac
    36601 agtggtgaaa accaggtaaa tcacgccaca gacgatggcg aaatagaacg gttcccaggt
    36661 acttttgcct gccagttggg tggctttgac cacatcttcc aggccgagta acgaaaccag
    36721 tgcggtagat ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat
    36781 catcgccgga aacatgatcc gccgaaacac ttgcccacga gtaaaaccga acgccgtcgc
    36841 cgcctctata tgtcctttcg gcactgccat aaaagcacca cggaacgttt cggtaaaata
    36901 agcaccgtag ataaaaccga gagtgataat accagcgacc atcggatcga tatcaatctg
    36961 cccgacgccc atcgcctccg tcaccgtgtt tagcgcaatc tgcaaaccgt agaaaatcag
    37021 cagcatcaac actaagtccg gcacgccacg aatcagcgtg gtgtaccctt cgaaaatcag
    37081 ccccgaaagc cgattttgcg agagcttacc gccagcgcca attaaaccga tgattacagc
    37141 gagcactaca gagctgatag ccagctccag cgtgacgagc gcaccctgta aaataacacc
    37201 tgaaaaccca tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc
    37261 tccctccggt tacccgaagg gagcgggctt tctttcaccg atggcgatta gccaccataa
    37321 acatcaaaat cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg
    37381 gcaaaggctt tgttcagtgc ttcgcgcagt tcgttatctt ctttacgcag gcccatgccg
    37441 gtccctacgc caaacagttt ttcatcttta acagacgggc caccgaattt gtaatcttta
    37501 ccgacaggtt gtttgaggaa accttcgctg gcagcgacct catcctggaa cgcggcatca
    37561 atacgtccgg cagtcaggtc agaataaatg ttgtcctgcc cctgatacga gacgatttca
    37621 atgccttttg gtgcccaatg ttcattaccg aacgtctcct gggtggtgcc ctgcaatacg
    37681 cctacccgtt tgcctttcag cgactcgact gtcggctgaa tgtcagaatt tttcgccacc
    37741 accaaacgag aatcggcagc gtacagtttg tcggtgaagg ctatttcttg ctgacgtttt
    37801 tccgtaatgg aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt
    37861 aacgcatcca gcggattttc gacaaacgta cattgcgtat tgatgcgttt gcataattcc
    37921 tttgccagat cgatatcgaa gccaaccagt tcgccttgtg aattttttga ttcaaatggc
    37981 gcataggtcg ggtcggtacc gatgcggatg ttttgcggaa tcgcagcaaa tgccgcagtt
    38041 gcgctggaga aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca
    38101 actgacagtc tttttatagg gaatatttac aggtttgtta cacttatcgt gccataaaat
    38161 tgaccatcaa ggcaaaatat tccgccccgt tagacgtatt atttttcatt aaaaatgctt
    38221 aagtatgcat ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc atgatgatca
    38281 ttccatcagg tacagcttcc cagcgacgta tcagtcaccg tagacattaa agtcgaaata
    38341 ctttttcgcc atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg ccttattgaa
    38401 ggcagccgtc agttcagcat catctttacg tagccctaca ccggtgccat caccgaagta
    38461 ttttttgtct tttactgatg agccagcaaa ggcgaaagct ttaccagcag gttgcttgag
    38521 gaatccttcg ctggcagcaa cttcatcttg taacgcagca tccagacgtc ctgcagccag
    38581 atcggaatag accaaatcct ggttggcata ggccaccaca tccacgcctt tgctacgcca
    38641 ggtctcgtta gcgtaagctt cctgggttga tccctgcagc acaccaacat gtttaccttt
    38701 cagtgaatcc agcgttggct gaatcggtga acctttggca gcaatcaaac gagaatctgc
    38761 ggcgtacagc ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa tggaaagcga
    38821 cgaaataatg gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcact
    38881 ggcaacccag gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc
    38941 aaagccaaca aaatcacctt tagcatcttt cgacgagaac ggcgcatagg tggtatcggt
    39001 tccgatacgt accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac
    39061 taacaaagac agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt
    39121 tttatgttgt gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct
    39181 tgacgacaac agtaacattc aacgttaaat atgttaataa gacgttgcat tattgtcctg
    39241 aagttgaaga tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat ggcagaaaca
    39301 aaatgtcgag gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcggagtt
    39361 atgcgccctg ccagcgggca aagagatctt caggaagggt tattgcaaac tggtcaagaa
    39421 cacgattaac cgtctgattt atcacatcat caagggattg cgggcgatga taaaacgccg
    39481 gaacgggagg cataatcacc gcaccgattt ctgccgcctg agtcattaaa cgcagatggc
    39541 ctaagtgcaa tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct ttcagcacca
    39601 catctgccgc acgggtcagt aagccatcag tatagctatg gacaatgccg gaaagggttt
    39661 tgattgaaca gggtaaaatc accatcccca gcgtctggaa agaaccggaa gagatgctgg
    39721 cggaaatatc gcgcgcatcg tgcgtgacat cagctaatgc ctgcacttcg cgcagggaaa
    39781 aatccgtttc gagggataag gtctggcgcg ccgcctggct catcaccaga tgcgtttcga
    39841 tatctgtgac atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg ccgctggcac
    39901 cgctgatgcc tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac
    39961 tttgcagtat ttcggcggga gttgcaagtc agggtgccag accggcaccc tcagcgaagg
    40021 catcatcctt cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg
    40081 gcgtcatcat tacgtaacgt atcgaggaaa tcgaggtagc cctgatcaac atctttggtg
    40141 acgtagacgc cgttgaacac cgagcattca aactgctgga tatccggatt ttcagcgcga
    40201 acggcgtcga tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg
    40261 cgaatttcat caacttcgcg accgtgagcg atcagttccg tggcgctcgg catatcaata
    40321 ccataaacgt tcgggaagcg aatttccggt gccgcagaag cgaggtacac tttcttcgct
    40381 ccggcttcgc gtgccatctc gataatctgc tcagaagtgg tgccacggac gatggagtcg
    40441 tcgaccagca ggacgttttt atcgcggaac tcggcgcggt tggcattcag tttacggcgc
    40501 acggacttac gacgcagctg ctggcccggc atgataaagg tgcggccaac atagcggttt
    40561 ttaacgaagc cctggcggta cggtttgccc agaatacgag caatttccag cgcgatatca
    40621 cacgaggttt ctgggatcgg gatcaccacg tcgatatcca gatcttccca ttcgcgggca
    40681 attttctcgc ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga aattttgtcg
    40741 atgaacgagt ccgggcgagc aaagtataca tactcaaaca ggcacggatt gctgaccgga
    40801 ttgtcagcac attgacgggt aaacaactgc ccttcttcag tgatgtaaat cgcttcgccc
    40861 ggcgcgacgt cacgcaggaa atcaaagccc agcgtatcga gcgctacgct ttcggaagcg
    40921 accatatatt ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag cggacgaatc
    40981 ccgtttggat cgcggaaagc aaccataccg tggccgataa tcatcgccac acaggcatat
    41041 gcgccgcgga ttaagcggtt tgtggcagca atggcagcga aaatattgtc ggcttccagc
    41101 gggtagtggc ggaagttgtc cagctcgcta gcgaagatat taagcagaat ttccgagtcg
    41161 gaagtggtgt tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg
    41221 ttggtcagat tgccgttgtg ggcaagcgta atgccatacg gggagttaac gtaaaacggc
    41281 tgcgcttcag aggcgctgga gctgccagcc gtagggtaac gcacatgacc aatgcccata
    41341 ttgccctgca aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct
    41401 ttacgcaaac ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg
    41461 cgatgctgaa gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg
    41521 ataccgacaa taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac
    41581 gctctgggca agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa
    41641 ctgaactgcg ggatcagctg tgatttgctc cagtcttcgc ttttcgacac cccggtaaag
    41701 gagtcgagaa agaagagaat ggcggcaaca atcaacacac cgcgcaacgc accgaaacag
    41761 acgcccagca cccgatcggt gcctgacaac cccgttttct ccaccaactg gcctatcacg
    41821 aagttcacga tagcaccaac aatcagggta gcgataaaca gtaccgcgat ggcaattcca
    41881 tttcgaacca gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga
    41941 ctggcaacaa agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg
    42001 cggatcaggc taaccagaga ggaaaaagca atcaccgcga ttatggcgta atcaatccag
    42061 accatatgtg tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa
    42121 aaagaaaacg tttgcgtagg gatttccttc ccacgcatca ataaaaatgg cgctgaaaaa
    42181 atattcaacg ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt gtcggatgcg
    42241 gcgtaaacgc cttatccgac ctacggttct aacactgccc aggcctgata agacgcgcca
    42301 gcgtcgcatc aggcaagact gtattaattc ggcgtatagc ccattaccac gccacttaag
    42361 ccagaaagtt gcttcaactc acccagcgaa cctttcagct tatctttcga ggcatccggc
    42421 ccaaccagaa tacgggtaat tttaccctgc actggcgtgg atggcgacgt ataaacccga
    42481 taaccggcac cgcgcagctt accgacaatc tcattcactt tatcggcatt tttcagcgca
    42541 cccagttgca caacataggc tttacccgtt ggcgctgctt tttcttccac gaccggcttc
    42601 ggttctggct tcggcgcagg tggtgcttca accttcggtt taggcggctc caccggtttc
    42661 ggcttcggtg gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt ggctgcaata
    42721 gtggccggat cgagcgacgg tgctgcggca tcacctgccc gcacctcttc cgctgcgcct
    42781 tccggcggct gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg ctcatcgcga
    42841 tcgcccgctt ttggaaccag cgggatagcc gcaaactcat cctgataatg ttttttctgc
    42901 ccgtccagca gccctggaag tacaatcacc cccagcgcca ccagcacgat cgtgcccact
    42961 aaccgattct gaaacttact tgccaccgct tctcctcgcg tcaatcactt ccatgacatg
    43021 tgcgaccgtg tggaaagaac cacacaccag cacggtgtct tccgctttag cgtccgccat
    43081 tgcggcatcc catgcctgcg caacgctatc aaatgatttg ccgttaccca aatgctcaag
    43141 cagttgttct gccgtggcac cgcgcggccc ttccagtggc gcacaatacc agtcatcaac
    43201 cacgcttttc aaccaggcca gagttccggc aatatcttta tcatgtagca taccgataac
    43261 cgccagcacg cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat attccgccgc
    43321 atgtggatta tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa tctggaaacg
    43381 tcccggcaaa attgcgctgg caatcccgtc gcgaatggca ttttcactga cttccagccc
    43441 gctggcacgc agtgccgcca gcgctgttgc ggcattcggt tgcgagacaa gcggcaacgg
    43501 cagattttcc agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg tgacggaata
    43561 gttccactca acgccccgac gttgtaacag tgcacctttt tcctgcgcca catcagcaat
    43621 ggtagaaggc atttccggct caccgacaat tgccggtttt gcgctgcgaa agatgcctgc
    43681 tttctcgcga ccaatacttt cgcgatctgg tcccagccag tcggtatgat ccagcgcaat
    43741 gctggttact acagccacat cggcatcgac aatattggtt gcgtccagac gaccgcccag
    43801 ccctacttcc agaatcacta cgtcaagttg tgcctgtttg aacagccaca acgccgacag
    43861 cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa
    43921 agaggcggtg tgggccgatt ccggcaattc ctgcccctgc acacgtacgc gctcggtata
    43981 acgcaccaga tgcggtgaac tgtagacgcc cactttgtac cctgccgcca tcagaatcga
    44041 ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg
    44101 cgctggtttc aggacgccaa gacgcgccgc gacctggctc acgcgctcaa ggccgagatc
    44161 gatagtttta ctgtgcaggt tttccagata agaaagccac gaagccagag gcgacgcggc
    44221 ttgaggagtg cgtttgataa tcatggtatc cgctgattcg ttacggtgag aatagcaaaa
    44281 gggcagagcc agtggccctg cccttatcag ttatcaggcc tcaggttcct gatccggtac
    44341 cgggggtacc actacgcctt cacgcggcgc ttcaggattc ggcgctggca gattcatcaa
    44401 cttcgccaga atgctcgcca gtttcaggcg catttccgga cgatggacga tcatgtcgat
    44461 ggcgcctttc tcgatcagga attcactgcg ctggaatcct ggcggcagtt tttcgcgaac
    44521 ggtctgttcg ataacacgcg gaccggcaaa gccaattaac gctttcggtt cagcgatgtt
    44581 gagatcgccc agcatggcga aacttgcaga aacaccgccc atcgtcgggt cggtcagcac
    44641 ggagatgtac ggcaagccgc gctcctgcat ttttgccagt gccgcagagg ttttcgccat
    44701 ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg agaagcagat
    44761 cagcgggcag ttatcttcca gcgcctgctc aacggcacgc acgaaacgtg cacccacaac
    44821 agaccccatt gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc
    44881 atacagagtg cctttcatca ccaccagcgc atctttttcg ccggtttctt tctgcgcaga
    44941 agccagacgg tctttatact tcttggagtc acgaaacttc agcacatctt tcggctcaag
    45001 ctcgctaccc agctccacaa ggcttccttc atctaacagg ctatgcaggc gattacgcgc
    45061 tgtcatacgc atgtgatggt cacacttcgg acagacctca agattacgtt ccagctcagc
    45121 gcggtataaa acctgaccgc agctatcaca cttagtccac accccttcag gaatgctcgc
    45181 cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc cagctcatta gggacctttc
    45241 tgtctgaacc tggttcgatg ccagttttat ctttggggac gcataatgcc atttttgccc
    45301 ccaacagacc atgaatgttg cacattaaaa cataacagcc cgaaactttg gataaaaaag
    45361 tggtcgaacc gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg tttgtgacga
    45421 ataaattcga aaaccccagg caatattgaa acaaaaataa tgccaacaat catcagctta
    45481 aggttgctct gaatgaaggg gagcgtgccg aagaaataac ccgcgtaggt aaaaatcagt
    45541 acccacaaca gtgcgccgat cacgttataa gcggcgaaat gacggtacga catgtggccc
    45601 attcccgcaa caaacggcgc gaacgttctg acgatcggta caaaacgggc gagaataatc
    45661 gttttgccgc catgtctctc gtaaaactga tgagtcttat caagataact gcgacgaaag
    45721 attttcgaat ccgggttact gaacagtttg tcaccaaata accgtccaat ggtatagttt
    45781 actgcatcac cgacaatagc ggcaatcaac ataagcacca ccatcatatg gacattgaga
    45841 tcgttagttt ccagcgacgc caatgcacca gcaacaaaca gcagcgaatc accaggcagg
    45901 aatggcgtta ccaccagccc cgtttcgcag aacagaatca aaaacagaat ggcataaacc
    45961 cagacgccgt actccgcgac cagctctgcc agatgcacgt caatgtgcag gataaaatcg
    46021 atgaggaaat aaatcaggtc catatttgcc tatgccttgt actcgtcatc tttcaggctg
    46081 taactacgtt ggctacagcc tgaaatgttc cgagcattat tctcgttagt ccgccagaaa
    46141 tagcgggccc attggcggtt ttggaagatc ataccggtca gggtaatcca ccgcgaccag
    46201 atacagccct tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg ccgccagcaa
    46261 ctctgctatc catctctccg gctggttgtg ggcaccgact tccatcaggc tgccgacaat
    46321 attcctgacc atatgatgta caaaggcatt cgctttgata tctaccacca cataaggacc
    46381 gtgacgcgtg acattaatat gcataacgtt gcgccacggg gttcgggact ggcactgcac
    46441 cgcacggaac gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc gatgcatccg
    46501 ttcagcgtcc agcggttcgt aaaaatgggt taccccttta ctcaataccg ccgggcgcag
    46561 ccgatgattg tagatgatgt agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa
    46621 atcatcaggt acagctttaa cccaacgcac ggcgatgtca ccaggtaaat tcgcatttac
    46681 gcccaaggtc cacgccgcgt ctttgcgctg cgcggttgtt tcgaaatgca ccacctgccc
    46741 ggtaccgtgt acccctgcgt cagtacgccc ggcgcagaag acggtgatgg gttcgttcgc
    46801 cacctgggag agcgcctttt ccagcttctc ctgcacactg cggacttcat tctgccgttg
    46861 ccagccgtaa tacttactgc cgtcgtactc aatgcccagc gcaattttat aaactggcag
    46921 ttgttgctgg tcggacatta gtacagatac tcctgcacca gtttctcggc gatttttact
    46981 gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg accagaactg gacttgctcc
    47041 ggcataccgt agtcattacg cacgcagcca acagaaagat gcggcgtacc cgaagcatcc
    47101 cctacctgag tcgggaattc gttctcttca gagagcacaa tatcttcgcc ctgagcaaac
    47161 gcatcacgcg cttcttctgc cgccagcgga cgcagtgctt caaagttgac catctgggca
    47221 tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc
    47281 aggattttgc gtacttcgtc aacgatacga cgttcttcac gcacgctacc ttcgctatcc
    47341 ggcaacaacg gcagcatatt gaacgccagt tgacgaccga agaaatcttc ttcgtcaatc
    47401 ggaatgccgt tgagcaattt cgcactctgc cccgctaacg catcgaccgc ttttttgccc
    47461 tgggcggagg ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc ctgatcgatt
    47521 aacggtttca gtgccgccag cagctggctg gtcagactgt ctggtacggc gatgacattc
    47581 cggttgcggt aatcggtcag tacaaacggg tttacttccg gcaccaccag cggtacgtcg
    47641 ggttcgagag caaacagtcc gctgctgtcg atcaccaggc aacctgagtt ggtcgcttct
    47701 tcaacccagg cagcggtagc ttctttgcct gcgacaaaaa atgccagctg cgcctgcgtc
    47761 cagtcgaatt cagcggcatc ctgcacagtg attgttttac caccaaagcg cagttgttcg
    47821 cctgcgcttt cgttacgtgc cagtgcataa atttccccaa ccgggaactg acgttcagcc
    47881 agcgtttcaa gcagggcttc gcccacagcg ccagttgcgc ccaggacggc aatgttccag
    47941 ccttcagaca tggtggttta ctccagaaat agcaaagctc cctgccaatg ttacagcaga
    48001 gagcatgaag aagagattaa cgtgccggat gatgaacggc gttaaaaccc agtttacaca
    48061 gcaatgatgc cgcactggcg tcatcacaaa ttacatacag agatgaccat tcacggcgct
    48121 caagatagtt tttgcgcagt ttatcgaact cacccggtat cccggcgact ttacgcagcg
    48181 gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag ccttttcagc gtcggttgat
    48241 cgagcgggcc atgcagcgta atgcgaccaa actctggcgc gggcagtaat gtatccagcg
    48301 caacgtgctg ttcatgccca ataaacttgc tataggcttc aaacacttgc gtagtaccgc
    48361 gtgctttacc ttccagggta taacctgcga tatgcggcgt gccgatatcc acttttgtca
    48421 gcaactccac gttaagttcc ggttcgcctt cccagacatc cagcactacg cttaacttct
    48481 ggccttcatt caggcaggtc agcaacgcag tattatcgac gactgcgcca cggcaggcgt
    48541 taatcagaat cgctcccggc ttcaggctac ggatcagttt ttcatccgcc agatgcagcg
    48601 ttttgtacgg cccatctttg aagagtggcg tatggaaagt cagaatatcc gcatgctgaa
    48661 ctaattcatc cagcgagcgg aaatcaccct catccccacg gtcagcgcga ggcggatcgc
    48721 aaagcaatgt tttaatccct aacgcttcca gtcgcgcctg caatcgacgt ccaacgttac
    48781 caacgcccac gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca
    48841 tcagtagtga ggagaaaaca tattccacca ccgcaatcgc attacagcca ggtgcagcgg
    48901 aaaaaccaat tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc cccgcagtgg
    48961 cagtgccaac aaatttaatg ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg
    49021 aacgcaccat cagcgcatcc gcgtctgcca gttgagcgac ggggattgga cgcccgggaa
    49081 ccgcggtcac ctcacccaaa cggctaaata agtcgcgggc ataaggcata ttttcatcaa
    49141 caaggatttt cacgtttgtg ttacctgtat gagacgagag ttaaccggac aagtgtgcca
    49201 taatctcgcg gccaggcata cttgcgaaga tttcaggtat aaggatacgt aatgatacaa
    49261 cctatttccg gccctcctcc tgggcaacca ccaggtcagg gagataacct gccgtctggc
    49321 gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa
    49381 gtgacctcac tgacgcaaca acaaagagca gaactgtggg cgggtatcag gcacgatatt
    49441 ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc ctgccgctga gcataatctg
    49501 gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc gccagctttt agcgcaatta
    49561 ggggagtatt tacgtctggg gaataatcgt caggcggtca cggattatat ccgtcataac
    49621 tttggtcaga cgccgctgaa tcagctctca ccggagcaat taaaaaccat tctcaccctg
    49681 ttgcaggaag ggaagatggt tattccgcaa ccacagcagc gcgaggcgac cgaccgtcct
    49741 ttattaccgg cggagcacaa tgcgctaaaa cagctggtga ccaaacttgc ggcggcaacg
    49801 ggggaaccca gcaaacagat ctggcaatcg atgctggaac tttccggggt gaacgatggc
    49861 gagttaattc cagcgaaact gtttaaccat ctggtgacct ggttgcaggc gcgccagacg
    49921 ttaagccagc aaaatacgcc gacgctggaa tcactacaga tggcgctaaa acaaccttta
    49981 gatgccagtg aactggcagc gttatcggcg tatatccagc aaaaatatgg tctttctgcg
    50041 caatcatcgc tttcttctgc ccaggccgag gatattctta atcagcttta tcaacggcgg
    50101 gttaaaggga ttgatccgcg tgatatgcaa ccgctgctta atccttttcc accgatgatg
    50161 gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga tactgttagt cgcgattatc
    50221 ctgatgctgg tctggctggt tcgttagccc cgacgaaatg acagtatcgt gacaataatt
    50281 cccagcaccg cagagatcgc cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc
    50341 agcattcccg ccagcggccc ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg
    50401 cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac gcccagcgca
    50461 ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc gacccacgca
    50521 cctggggctt gccagagcag caacaagccc accgtttcta caagcagaga gacaatcgcc
    50581 actttcacgc cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat cacgacaaat
    50641 gcgccgccaa actcggtaag agtaaagccc gccatcgccc atcctttgct ggcaaagtag
    50701 agcgaaacga aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag ccctaaccct
    50761 ggtttccaga taaacccgac aacgctccac agcgatggac gttctcccgc cagggccggt
    50821 actttgcgca ctgtgccgtt acaggcccac gccagtaagg gtaataccat tgtggtgatc
    50881 gccagtgcgg caaaaccgta atggctatga atcaacaggc caagcggagc accaacagcg
    50941 agggcaccgt aaatcgccat tccattccat gacatcactt tgccagagtg ttttggccct
    51001 acgatgccta acccccaggt cagagcgcct gtcagtaact ggctttcacc aaacccaaga
    51061 atcaaacgcc cgatgaccaa cagggcaaat ttgaacggtg cggagacagg caaaatcgcc
    51121 gccagcagca acgcgccgcc agccagacca caagctaaca tcccctgaag cgccgaacgt
    51181 tttgcgccat attgatcggc cagtcgcccg gcatagccac gcgtcagcac cgtagccaga
    51241 aactgaatcc cgacggcaat gccgaccatg gtattgccat agcccagttc atgatgaaca
    51301 aacagcggga taaccggcaa cggcagccct acggtcatgt aggtgagaaa aaccgcaaaa
    51361 gcgatgcgga agagcgaaaa attggcagaa gatcgtgttt cggtttggct tacagcagtc
    51421 atgcattact ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc tctctggttt
    51481 cagggttacg gtgcgttggc aggatttaac gcgtacgtct tttcagaagg aaatcgacaa
    51541 agcgggaagt ttgcctggaa ctggtggcga ttgtcaatga tgtgaaaaag ggaaccatca
    51601 ggttcccttt tgcgttagtg ccggaggccg cattggcgcg taacgtcgga tgcgacgctg
    51661 gcgcgtctta tccgacctac tccggcctac tgcgaattaa tctttcagct tgcgcattac
    51721 cagcgtggcg ttggtgccgc cgaagccgaa gctgttagac ataacagtgg tcagttcgcg
    51781 atcggtcgtt tcggtcacga tgttcagacc cgcagcctgc tcgtccagct cttcaatgtt
    51841 gatgctcggg gcgataaagc cgtgttccag catcagcaga gagtagatag cttcctgcac
    51901 acccgcagcg cccagtgagt gaccggtcat ggctttggtt gcagaaatcg ccgggctctt
    51961 atcgccgaac acttcacgga tagctgccag ctctttcacg tcgccaaccg gagtcgaagt
    52021 accgtgagag ttcaggtaat cgattggggt atcaacgcca tgcatcgcca tctgcataca
    52081 acgtactgcg ccttcgccag acggagcaac catgtctgca ccatcagagg ttgcgccgta
    52141 gccaacgatt tcagcataga tgtgagcacc acgcgccagc gcgtgttcca gctcttcaac
    52201 cactaccata ccgccgccgc cagcgataac gaaaccgtca cggtgagcgt cgtaagtacg
    52261 ggaggctttt tccggggtgt cgttgtattt agtagacagc gcacccatcg cgtcgaattc
    52321 gcaggccatt tcccagcaca gctcttcgcc gccgccagca aacacgatgt cctgtttgcc
    52381 cagttggatc tgctctactg cgttaccgat acagtgtgcg gaagtcgcac acgcggagct
    52441 gatggagtag ttaacgccat gaattttaaa cggcgtggcg aggcaggcag aaacgccgga
    52501 tgccatcgct ttggtgacca catacgggcc aaccgctttc aggccgcgcg ggccgcgcat
    52561 tgcgtcagcg ccgaatacct ggaaacgcgg ggaaccgccg ccagaacctg caatcaggcc
    52621 aacgcgcggg ttattctggt aagcttccgg agaaaggccc gcatctgcga ttgcctgctc
    52681 catagaaagg aatgcataaa tggatgcgtc gctcataaag cgcacaactt tgcggtcaat
    52741 gaggccagtg gtatccagtt ttacgttgcc ccagacgtgg ctacgcatgc cggaatcctt
    52801 cagctcctga gagaaagtga tccctgaacg tccttcacgc agagatgcca ggacttcctg
    52861 ctggttatta ccgatgctgg aaacaatgcc caggccagta atcactgcac gtttcattca
    52921 atacctctgt aagtcgcaca tagagtaagt ttcgaatgca caatagcgta cacttgtacg
    52981 ccgaacaagt ccgatcagcc atttaataga gaaatttgcg cagccttaca cacatcgcta
    53041 agatcgagcc accgcctgta agacgagtaa cttacgtgaa acactactcc atacaacctg
    53101 ccaacctcga atttaatgct gagggtacac ctgtttcccg agattttgac gatgtctatt
    53161 tttccaacga taacgggctg gaagagacgc gttatgtttt tctgggaggc aaccaattag
    53221 aggtacgctt tcctgagcat ccacatcctc tgtttgtggt agcagagagc ggcttcggca
    53281 ccggattaaa cttcctgacg ctatggcagg catttgatca gtttcgcgaa gcgcatccgc
    53341 aagcgcaatt acaacgctta catttcatta gttttgagaa atttcccctc acccgtgcgg
    53401 atttagcctt agcgcatcaa cactggccgg aactggctcc gtgggcagaa caacttcagg
    53461 cgcagtggcc aatgcccttg cccggttgcc atcgtttatt gctcaatgaa ggccgcgtga
    53521 cgctggattt atggtttggc gatattaacg aactgaccag ccaactggac gattcgctaa
    53581 atcaaaaagt agatgcctgg tttctggacg gctttgcgcc agctaaaaac ccggatatgt
    53641 ggacgcaaaa tctgtttaac gccatggcaa ggttggcgcg tccaggcggc acgctggcga
    53701 catttacgtc tgccggtttt gtccgccgcg gtttgcagga agccggattc acgatgcaaa
    53761 aacgtaaggg ctttgggcgc aaacgggaaa tgctttgtgg ggtgatggaa cagacattac
    53821 cactcccctg ctccacgccg tggtttaatc gtacgggcag cagcaaacgg gaagtagcga
    53881 ttatcggcgg tggtattgcc agcgcgttgt tgtcgctggc gttattacgg cgcggctggc
    53941 aggtagcgct ttattgcgcg gatgaagccc ccgcgctggg tgcttccggc aatcgccagg
    54001 gggcgctgta tccgttatta agcaaacacg atgaggcgct aaaccgcttt ttctctaatg
    54061 cgtttacttt tgctcgtcgg ttttacgacc aattacccgt taaatttgat catgactggt
    54121 gcggcgtcac gcagttaggc tgggatgaga aaagccagca taaaatcgca cagatgttgt
    54181 caatggattt acccgcagaa ctggctgtag ccgttgaggc aaatgcggtt gaacaaatta
    54241 cgggcgttgc gacaaattgc agcggcatta cttatccgca aggtggttgg ctgtgcccag
    54301 cagaactgac ccgtaatgtg ctggaactgg cgcaacagca gggtttgcag atttattatc
    54361 aatatcagtt acagaattta tcccgtaagg atgactgttg gttgttgaat tttgcaggag
    54421 atcagcaagc aacacacagc gtagtggtac tggcgaacgg gcatcaaatc agccgtttca
    54481 gccaaacgtc gactctcccg gtgtattcgg ttgccgggca ggtcagccat attccgacaa
    54541 cgccggaact ggcagagctg aagcaggtgc tgtgctatga cggttatctc acgccacaaa
    54601 atccggcgaa tcaacatcat tgtattggtg ccagttatca tcgcggcagc gaagatacgg
    54661 cgtacagtga ggacgatcag cagcagaatc gccagcggtt gattgattgt ttcccgcagg
    54721 cacagtgggc aaaagaggtt gatgtcagtg ataaagaggc gcgctgcggt gtgcgttgtg
    54781 ccacccgcga tcatctgcca atggtaggca atgttcccga ttatgaggca acactcgtgg
    54841 aatatgcgtc gttggcggag cagaaagatg aggcggtaag cgcgccggtt tttgacgatc
    54901 tctttatgtt tgcggcttta ggttctcgcg gtttgtgttc tgccccgctg tgtgccgaga
    54961 ttctggcggc gcagatgagc gacgaaccga ttccgatgga tgccagtacg ctgacggcgt
    55021 taaacccgaa tcggttatgg gtgcggaaat tgttgaaggg taaagcggtt aaggcggggt
    55081 aatctgctct cgcagtgttt gccggatgcg gcgtgaacgc cttatccgac ctacgtgtga
    55141 cgtgtaggcc tgataagacg cgataagcgt cgcatcaggc atggtgctcc agacgccgca
    55201 acttactgct gtgacgcctg ctgaaacagg ttttcccaca tatcggtcac cagcgcctgg
    55261 tcacgcggcg acaattcacc ggcaccaatg gctttttcca gactctggct aacggtcgta
    55321 tgcaccgcct gagcggagtg gtcatcacca ctttccagtt ctgcgatggc taatgtcagg
    55381 tggccacgca aatacccact ggcaaacaac tcatcatcac tggcatggtc aaccatgccg
    55441 tcgattaatg ccagaatgcg tgattcaaac tccgcgatca tcttctttcc tcattgtaaa
    55501 acgtggcacc ggcttcagtt gagcgcttcc ggccacggga actgttccgc cgtaagttcc
    55561 ggcgtgtgat aataattttg tagtgcttta atgaagcgtg ccggacgctc gggaatgcca
    55621 tttgtcagat aatccatcac ctgcgcatgt acccgccgtt gaaacacaac acgatccggt
    55681 tcgaagtcgc cttccagatt gtcgcaactg acgttaaacg gatatcccgc cgccacacag
    55741 aacaaccagt cgagtgcctg cggcttcact tcaacatctt caaactggct ttgcgtttgg
    55801 gcatcacgcc catccgggca ataccagtag ccgaaatcaa ccagctcacg gcgcgctttt
    55861 ccggcaatac accagtgcga aatctcatga attgcgctgg cataaaagcc gtgggcaaag
    55921 acaattcggt tatacggtac ttccgcatca gcaggaagat agatcggttc gtcgtcgcct
    55981 ttaatcagac gggtattaaa ttcatcggca aagcagctat taaaaatttc aatcaactgc
    56041 tcatagtggt gtgtactgtt cattagttca tccccaacca gtggaggatc tcctgtccgt
    56101 ggctatcgta aagtaatttg gcactcatca ccgccgagac gataacaatc atcgggcgga
    56161 tcagcttttg tcctttgctc aacaccagcc gtgaacccat gcgcgcgccg aggaactgcc
    56221 ccaccagcat gacaaagccc gtcgcccaaa tcactttgcc gccgagaata aacagcagca
    56281 gaccgccaat gtttgacgtt gcgttgagta atttggcgtg agccgtggct ttggcgaggt
    56341 tgaatccgca cagcgtaacg aaggccagcg cgtaaaacga tccggcagcc gggccaaaga
    56401 atccgtcata aaaaccgacg cagccaccgg caattaacgc gaagggtaaa ccgtacatcc
    56461 ggcgctggcg atcttcttca cccagctttg gcatcagcaa gaaatagagg ccgatacaaa
    56521 tcaccagaat gggcaaaatc tgccgtaaga catcagcctg aacgtactgt accaacagtg
    56581 cgccgctcat tgagccgaca aaggtcatgg caatattgag tttctgatcg cttaaactaa
    56641 ccactttgcg gcgaataaag tagatagtgg cggaaataga gccgccgcag gcttgcagtt
    56701 tattggttgc cagcgcgtta gcgggagaca ttcccgcagc catcaatgcc ggaatggtga
    56761 gtaacccacc gccaccggca atcgagtcga taaatcccgc cagcatggcg acaaaaaaga
    56821 gaactcccag caacagcggg gaaaccataa acaggctatt aaacgtttcc attagatcac
    56881 gtgctcatcc agtagcgcct ggcaggaagg cggcaacgga ggcggtgtct tcttctcagg
    56941 ctttgttgtt cccggtttta gaggtgcaaa ccagctttgc agttctgccc cgcaaccatc
    57001 gcctgatggc ggtaaaggtt gatcttcaca ctccagacta tcggcagggc aacgtaatcg
    57061 tacatgcata tgcgcgcgat gctggaacca gggtcgcact ttgcgcaacc agtcgcgatc
    57121 ggtgcccgca tcaaggcaaa gttgttgttt aatcgccgga ttaacaaaaa tgcgcgtgac
    57181 gtctttgtcc tgggcagcga gtttgatcaa gctgaaaatt tctggcttcc acagcgtgga
    57241 gacaacgtgt ttaccgtcgc gggaaactaa gtccagtgct tgcgggcgca agagctgcgc
    57301 ggaggtccag cgagttttcg gcagttgcag aaagatatcg acatccagtc cggtctggtg
    57361 gctggcatga ccgccgttga aacgcccacc agcgggcatc cccatatcgc caatcagcac
    57421 cgtacccatg cccagattgc tcacctggct actcagacgc tggataaaca tcaccagatc
    57481 cgggtgaccg aaatagcgac gctgatcggt acgcatgacc tgataatgtt cggactgtat
    57541 cggcaacgtg tcagcgccga caatacagcc attagaaaaa ctgcctatcg attgtgcgct
    57601 acccggcaca ggttgggtta ttttttgcca cggcgtcgct gccaggctag cgctactggc
    57661 aagcagagcc agcagcgcaa tcgcggtttt gttcattttt taccagcgtg gaatatcagt
    57721 cttcacatcg gcattttgcg cccgctgccg taacaggtga tccattaaaa cgatcgccag
    57781 catcgcttca gcgatcggca ctgcacggat cccgacacag ggatcgtgac ggcctttggt
    57841 gatcatctca acttcttcgc caaagcggtt aatagtgcgc cccggcacgg taatgctgga
    57901 ggtcggtttc agcgccatat gagcaatgat ttgctgcccg ctgctgatac cgccgagaat
    57961 gccgcccgca tggttgctct ggaaaccgtc tttggtgatt tcgtcgcggt tctggctgcc
    58021 gcgcagcgcc actacgtcaa aaccgtcgcc aatttccacg cctttcaccg cgttgatgct
    58081 catcagcgca tgggcgatgt cggcatccag gcggtcaaag accggctcgc caagtccggc
    58141 ggggacacca ctggcaacaa cggtgacttt cgcgccgatg gagtcgccct cttttttcag
    58201 cgcgcgcatc agttcatcta acgcgtcgat tttgtccggg tccgggcaga agaacggatt
    58261 ttgttcaacc agcgaccagt ctttgatttc cagcggaatg tcgcccatct gggtcaagca
    58321 gccgcggatt tcaataccaa atttctcggc gagatatttt ttggcaatcg cccctgccgc
    58381 cacgcgcatg gcggtttcgc gggcggaaga acgtccaccg ccgcgataat cgcgcagacc
    58441 gtatttttgt tcgtaggtgt aatcagcatg gcctgggcgg aaaacgtctt taattgcgct
    58501 gtaatcctga gagcgctggt cagtgttttc gatcaacaag ccaatgctgg tgccagtagt
    58561 aacgccttca aaaacaccgg agagaatttt gacctgatcc ggctcgcggc gctgggtggt
    58621 atagcgcgat gtcccagggc gacgacggtc gaggtcatgt tgcaggtccg cttccgtcag
    58681 cggaatgcct ggcggaacac catcgacgat gcagccgagc gccagcccat gcgattcgcc
    58741 gaaggtggtt acgcgaaaga gttgtccaat tgtgtttcca gccatcacgg ctccgttatt
    58801 gttgtgtttg cgtgtttact taatctttat aaatcgcgaa atattcacgg gccgccagca
    58861 gctgctcttt ggtgagcata aacacaccat cgccgccgtt atcaaactcc agccaggtga
    58921 acggaacatc cggatattgt tccataagat gtaccatgct gttgccgact tcacaaatca
    58981 acacgccatc atcagcaagg taatctgccg cgtttccaag aatgcgacgc gtcagtttaa
    59041 ggccgtcggt gccagatgcc aggcccagtt ccggctcgtg acggtattcg tttggcaggt
    59101 cggacatatc ttccgcgtcg acatacggcg ggttagtgac aatcagatcg tactgcactt
    59161 tcggcaaatc acggaacaga tcggaacgaa tcggaatgac gttgtggatc agaccgtgtt
    59221 cttcgatgtt ctgttcggca accgccagcg cgtcgggaga gatatctacc gcgtcgactt
    59281 ccgcttccgg gaaggcataa gcacaggcaa tggcgatgca gccgctaccg gtacacatat
    59341 ctaaaatatg ctgcggttgc ttgctaatga gtccggcaaa tttattgttg atcagttcac
    59401 caatcggcga gcgcggcacc agcacgcgtt catcgacgta aaattcatga ccgcagaacc
    59461 aggctttgtt ggtcaggtaa gccaccggaa tgcgctcgtt gacgcggcgg atcacgcgtt
    59521 caacaatacg gtgtttttcg ctggaggtca gacgcgcggt gcgcatatct tccgggatat
    59581 ccagcggcag gtagagcgat ggtaacacca gctgtacggc ttcatcccac gggttatcgg
    59641 tgccgtgacc gtaccagata tttgccgcgc tgaagcggct taccgaccag cgcaacatgt
    59701 cctgaatggt ttgcagctca tttactgctt catcaacgaa aattttatcc acgtattcct
    59761 ccagggcatg atcgcaataa tttcggcggc tagtttgcca tgaagatgac gataaatcag
    59821 cattcacgcg cggtgagtga ggaaaaatac gtttaaaacg atcgattgtg ctacgagtcg
    59881 ggtaaactgt aggaaaatta gaaatagaga cagataaatg aaaaagaaaa caacacttag
    59941 cgaggaggac caggctctgt ttcgccagtt gatggcgggg actcgcaaga ttaagcagga
    60001 cacgattgtc caccgaccgc agcgtaaaaa aatcagcgaa gtgccggtga aacgcttgat
    60061 ccaggagcag gctgatgcca gccattattt ctccgatgag tttcagccgt tattaaatac
    60121 cgaaggtccg gtgaaatatg ttcgcccgga tgtcagccat tttgaggcga agaaactgcg
    60181 ccgcggcgat tattcaccgg aattgttttt ggatttacac ggtctgacgc agctgcaggc
    60241 caagcaggaa ctgggcgcgt tgattgccgc ctgtcgccgc gaacatgtgt tttgtgcctg
    60301 cgtgatgcat gggcacggga agcatatttt gaagcaacaa acaccgctgt ggctggcaca
    60361 acatccgcat gtgatggcat ttcaccaggc accgaaagag tacggcggtg atgcggcttt
    60421 gttggtgttg attgaagtgg aagagtggtt gccgccggag ttgccctgaa tgctggcagc
    60481 aaatgccgga gcagcgtaaa cgccttatcc ggcctacaaa tatgtgataa ttcaataaat
    60541 tgcaattcac ttcgtaggcc tgataagacg catcagagca atactacgaa ctacagttca
    60601 ttcaatacat actgaaaacg acacgtcgaa tcaatcggtt gcccactact ggtattcaca
    60661 ttcaccacaa tatcgtgtag tactttatcc gttaccggat gacgagttgg cgtaaccgtt
    60721 accgttgatt tatcacaacc gtaagaggta aaggttaaca attctgatac cgcgcggcag
    60781 tatgctcctc tgccatcatt atcagaaaaa ttactgccat cacagagtct gccgccttcc
    60841 atcaggtagg tcgaaagcgt ggtggtcact gttgaccccg tggactgaat tcgtaacgcc
    60901 ttattatggc tgacataatt ttcactccag gagatatcgt atgaagtgac cggtatcgtt
    60961 gggcatattg tgttatgcac tgtaggggcc gtatagcttc caaatccggg taaatctgta
    61021 taggaataaa aggatgcgat ccaagggcaa ccataatcat cagatgcaac ggtaatttta
    61081 aacgtcatat atgcacaacg aaaaattttc gtatctatgc cagggcaatt tgagttatta
    61141 attgtatcaa agtcagtcag taccaaagtt ggagctgaac ccgacccaga ccaggaaata
    61201 acccgcaacg tatcgccttt atacgcaaaa gaatcatatt tcccatatgt aacctccttt
    61261 ggcgttatca ccgtaacatt gaaaacactg ctcgctacag caccggttac cttgtccatg
    61321 gcataataaa ctctcacact gtcaaaactc gcactcataa gtttatctcc agcataactc
    61381 ttagatgcgc cgacaacact cagaacgagc agcattaggc tcgcgaaaat tatggtccat
    61441 tttttcattt cagaaatact caaaggttgc cgtcactgtg gcactaaagt ctccggacgt
    61501 tacatctcgc ccgctctttg cctgtaacca ggtattaaaa tttaggctgt tatttccctg
    61561 attcagcggg agcgtgacgc ctgttgtatt gttgattggc accgatgtgc ctgccgcagt
    61621 ttcaatacca atcccagcgc cggatgctga tgacgaggag tcaaaggcca gaaagccggg
    61681 tagagcacta tcttctgttc ctgtcaacgt tactttgaca ttaaacaatg tagaactatg
    61741 acaatctttc aattgaaaca ccaatggcac tcgctctgac tgcccaaggt tcattaaatc
    61801 gctggcggca atggcaggaa aatgtacttc tgccaactgg ctaccattaa tcactggcgt
    61861 acacgattta ctgattaaat tgccggtaaa gtgcaaatta tctcctgcta ttgtggttcc
    61921 actgcccagc aacatcaggc tactaataaa aaacaacgta cgctttttct tcattggtaa
    61981 tctacctgta acgtggccca ggcctcaaag tccgcctctg gcagaacgac accactcttt
    62041 tttactggca cagccgttaa caccggtaaa tcggtttcat tcaccataag tggcgtattg
    62101 atcgtaaaac tctgccctgc ctgtttgagc tgaatgccta aaccagtgat attgctgctt
    62161 actgcagcat tatcaaaatc gcttagtgtg ccactccagc tcaaggtcat ttccattaat
    62221 gggtcgcgaa ccgccgtatc gcaggtaatg gaatacggca cggtttgctc ataattgctg
    62281 ccattaatgt catcaataag cacatcctga aaagttattt caatggtttg agcgttgcta
    62341 atcgtgcagt taggcggctg cactaaataa ccgtgaaaag agacgtttga gagtgccatc
    62401 gagcaaaagc cccacagcaa aataagcgag atccgtttca ttggtaatcc accacaattg
    62461 tggcactggc gttaaattca gctgccgcta acgttgtacc actctgtttc accggcacag
    62521 cttccagaac gggaactccc gaggaaaaat taaatggcaa ccatgagcca gaggttaagt
    62581 cgagtattgt gtgatcacta cttttctgaa cccgaatacc aaacccagga atacccgtgc
    62641 tgataactgt ttcaccgtta atcacgaccg ttgtcgcctg catctgtaga cgcaggtcat
    62701 ccattgccag attgttacaa acaagcgagt agtcaatggg acgtttataa tcaaccccat
    62761 tgatcttatt aatgaataca ttaccaaact caactgccgc accatttacc gtacaaggag
    62821 gaggggcatc gacaagcacc cgtaaattca tacttactga cttactcgct gcaactgcaa
    62881 agccgctacc aacacaacat atcagtaacc cagttaccgc ccagacagaa caagatcctt
    62941 tcatactggc tctcccggcc tcagcctttg cggctctgct cttcatcgac ctggcaactg
    63001 ttaccgttgc aagtgaaaaa gagcggcatc cgagcgccat agtcgttgac ataggtcagt
    63061 accggcacgc tacccatctt cacattcagc ggtgcagagg ttttcggcga catcactatt
    63121 ggcgagaaac cggctgcagg attgccattt ttctgtgtgc tggcattact gaaaatgacg
    63181 taatacggcg tcgggttatt gacggtaaat tcctgaccgt tgcgggttaa agtaacctta
    63241 tattgccagg ggtttttcat gctgacgttc tccagcgcct tcggtcgcca gaacaactta
    63301 atacgcgttt gtagtgctat ttgcagcgtg ttggctttgt ttgattttgg cgggatctca
    63361 cgcacgttga agtagaacag gctttcacga tcggcaggca atttgttgat atccggcatt
    63421 ccctgcactt ttacctgccc gttcatcatt gaatcgatgc gttgcaccgg tggcagaaca
    63481 gttaacggtg aactgatctt gttacccttt tcatcttcga tccagctttg ggccagataa
    63541 ggcagtttcg gatcgttatt gcgcaacgtc acgctgattg atttgtcgct ttcattaaat
    63601 atcaaacggg tacgatctgg cgttacagaa gcctgggcga caaactagca gcgctaagca
    63661 gtaacgctag cgtcgtagca cccaatttgg cagagcaaag taaatcagac atggatcatt
    63721 ccttaatcac gttattggtt agctgaaaca gaaacagggg gattggtggg tgtcacctgt
    63781 tgcgtctgct cctgaatgac aggctttatg tcatcaggaa caaccggcgc gacgttgcct
    63841 ttatgctggc atggcagtaa caagccgtta aacagatcgg caggcagcgg gtccggcagg
    63901 ttgatatcgc aatgcgcaac accactccag aacacactca tgtgttcgcc aggtttcacc
    63961 ccagccagat aaacattgcc gtcatcatcg acaaggccca ctgtctgctc gttatcattt
    64021 tttacttctg cgccaaacgg aggatggctg ccgtcttgta aacgcagcac cgccatcgct
    64081 ttttgaccac tgatgacggc aaatttgcgg tagccaatcg ccccttcagt tagcgtggct
    64141 tgtaccaccg actgggttgc ttcggcgttt tccggcagtt tgttgaggtc gatatacgcc
    64201 tgattgcggt aatagttatt gacgtcagaa acgacggctt taccaaacat attggtataa
    64261 acagcagcac cgttaccttc taccggaaca tcggcaacgc catcggcatc aatcaacaag
    64321 cgtgtcccgc ccatattctg ggtacggtga agtgcgccac cgtgggcagt aagcgttgcg
    64381 ccgccctgta acgacaagcc cgcagaggtg tattgccctt catggtagtt cgcactgagg
    64441 tcaacctgcg ccagcgaacc atcatggctg taatagccgt caacgctggt gtgtttgtca
    64501 ctggtgccga cgttcaactg atagtgagtc gcgtcatcga cacggctgaa ataaccgacc
    64561 tgactgctgt ccgtcccact gccatagtta ccgttatagc taacggtact gttgtcaccc
    64621 cacggcatac tgagcgaaat gtacatgcct ttgtcggcct ggttgtcata ctcgtaacgg
    64681 tagccagtca tcgagatgct gacattacga atactgccca tattgaaata gtgcgagagc
    64741 atgatgttgt agtttgtctg ctcctcgcga tcccagtagg tatggcgggt atagttgaga
    64801 taaaccgaaa cacccgcatc gcggaagttc tggttataag tggcagtgta catctctttg
    64861 tcgttgcccg tgcgtaccat tccgctgtca ctggcatcca gatactcgct catggtcata
    64921 aagttctctt ccgagaagcg atatccagca aaagtaacgc ggctgttgag ctggtcaaag
    64981 tctttggaat aactcacacg gaaggagtta ccgtccagcg aacctttgcc ataagcggtg
    65041 tctttatcca gtttggtgtg cgagtgagta acatcaaacg cgaccgcgcc gaatgtagac
    65101 aaatcgcgac cgacgccaag cgccgcagac tgatagtttt catctcccag tgcgccgcca
    65161 tataacgacc agccgttagc gatcccccag gaagcttcag caccagaaaa aaattcaccc
    65221 tcgacatggt gtccccactc ttgcggacgg cccatcatga tcttatagcg aacctgacct
    65281 ggacgagtga ggtatggcat cgaggcggtg ctgatgtcat attcctgcac ctggccgttc
    65341 tgttcttcaa tgcgaatatg caacgtaccg gagacagaat caccaagatc ctgaatacga
    65401 aacggtccag ccggaacctg cgtttcgtaa atcacacgcc ccatctggct gacggtcact
    65461 tttgctgtgg tgtgtgccac gccggaaatg tctggcgcgt agccgcgcag attgggaggc
    65521 aacatttgat cgtcagtact gacactgcca ccaacatagt taaaaccatc aaaaatatcg
    65581 gatctgaggt aatcctcgcc cagcgccagt ttggctttta atgatggtaa cgcccgctag
    65641 gcatagtagc gactccactc ccattttttt tgagtttcat cgccgctgaa ttcatcgtca
    65701 tcattactgc gagtatgttg atagttagtc tgccagtcag cacgcatacg ccacggcccc
    65761 aggttaaccc cgaccgtccc gttgccgctg atctcgttac tatcatcacc gccattttct
    65821 tcgtgccggg tttgtgcgtt gatgctgtag tccgcgacga tcccggagat gccgtcatcc
    65881 caacgtgaag gcggatccca gtcgggataa gtatattcga ggtaagcctg tggcagtgaa
    65941 atgactaatg cggactggct taaatcagcc ttaatttcca tgccttccag ttgaccggat
    66001 ttcaggcatt tagcatcgtg gctccattgc agatttttcg ccacgtcttc ttttaaacca
    66061 aactgcgcta ccagttccgg tgtcagacaa gcatagcttt tgctcgcgtc atcttcacca
    66121 gcataccagt aaatatcgta ctcttccgcc aatggctgtt tatttagttg aacctgtaaa
    66181 ttgtattttc cgggctcaac atatccctgg ctggaaaaac gcttcagatc aatttttgtg
    66241 tcgcctttta attccagaaa acgggaatca aactgaatgt cgtcttcagc ccagacactg
    66301 ctataactcc ctgacattgc cagcgcaatg caccacggaa gaatccgtaa tcgaaaaaga
    66361 gaatggtcag gcatacacgt ttctatccgt ttttattctt gttcggtcaa aatccattta
    66421 aatcccccat agcagtgtcg ctatgggggt atgccttaca ggtaagtaat ctggaaggtg
    66481 gtatttgctt cgaaattacc cagatctggc gcatcagctg cgccgaccag ccaggcttta
    66541 aagttcagtg tctgcttcgc tttgccttta ttggtagaag tgtctttcac tattttctgg
    66601 tcaataccca tgccgctttt gtaagaggta cccagagagt caccgatcgc caggctaaca
    66661 ttgttaaatg ccgcacaggt atcggtattg aaaatggtgt aataattgcc gctatttgca
    66721 gaagaaacgg taccagtaaa ggtagtggtc attgtttcct gagtatcaaa tacgcaatct
    66781 tgcagacgaa tctggaaact tttcggcgtg gtggtgccgt tatttttcag tttttcggca
    66841 ccgatatcac ccatatcaac ttccagtttg tgatcgtccg gaacgataga acacactgag
    66901 tcttcaatgg tgccgtaaaa acgtgcagta ccgttgttac ccgcagcgat tgttgccgta
    66961 gaggtgaaca cgcccatcac cattgccgca gcaatagctg ttttaacaaa cttactcatt
    67021 atttgtcctt aaactataaa taattaaaaa tataaacatt gcaatacatt gattcagtca
    67081 ataaccaatg tttataccca gccattgaat acggcatgcg atccaaaaag aacaggtcgt
    67141 agaaaattgt attcgttggt gtggggccgc atagtgatgt ttccacaccc ctgatgcaat
    67201 ttaattttac taaagagtaa atgtagattt aatta
//
LOCUS       DAZPEP010000007        64597 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZPEP010000007 DAZPEP010000000
VERSION     DAZPEP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64597
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.007"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHUT9_000439"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="ACHUT9_000439"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFR1347008.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHUT9_000440"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="ACHUT9_000440"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFR1347009.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHUT9_000441"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="ACHUT9_000441"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFR1347010.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHUT9_000442"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="ACHUT9_000442"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFR1347011.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHUT9_000443"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="ACHUT9_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFR1347012.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHUT9_000444"
     CDS             complement(4809..7043)
                     /gene="relA"
                     /locus_tag="ACHUT9_000444"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFR1347013.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHUT9_000445"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="ACHUT9_000445"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFR1347014.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11211
                     /gene="barA"
                     /locus_tag="ACHUT9_000446"
     CDS             8449..11211
                     /gene="barA"
                     /locus_tag="ACHUT9_000446"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFR1347015.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLDLEPELLELLDEMDNVARE
                     ASKILG"
     gene            complement(11443..12783)
                     /gene="gudD"
                     /locus_tag="ACHUT9_000447"
     CDS             complement(11443..12783)
                     /gene="gudD"
                     /locus_tag="ACHUT9_000447"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFR1347016.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12804..14144)
                     /locus_tag="ACHUT9_000448"
     CDS             complement(12804..14144)
                     /locus_tag="ACHUT9_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFR1347017.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14146..15498)
                     /gene="gudP"
                     /locus_tag="ACHUT9_000449"
     CDS             complement(14146..15498)
                     /gene="gudP"
                     /locus_tag="ACHUT9_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFR1347018.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15933..16382)
                     /locus_tag="ACHUT9_000450"
     CDS             complement(15933..16382)
                     /locus_tag="ACHUT9_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFR1347019.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16400..17182)
                     /gene="truC"
                     /locus_tag="ACHUT9_000451"
     CDS             complement(16400..17182)
                     /gene="truC"
                     /locus_tag="ACHUT9_000451"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFR1347020.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17182..17511)
                     /locus_tag="ACHUT9_000452"
     CDS             complement(17182..17511)
                     /locus_tag="ACHUT9_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFR1347021.1"
                     /translation="MTTHDRVRFQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18133..18678)
                     /gene="syd"
                     /locus_tag="ACHUT9_000453"
     CDS             complement(18133..18678)
                     /gene="syd"
                     /locus_tag="ACHUT9_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFR1347022.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18746..19594
                     /gene="queF"
                     /locus_tag="ACHUT9_000454"
     CDS             18746..19594
                     /gene="queF"
                     /locus_tag="ACHUT9_000454"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFR1347023.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19706..21070
                     /gene="ppnN"
                     /locus_tag="ACHUT9_000455"
     CDS             19706..21070
                     /gene="ppnN"
                     /locus_tag="ACHUT9_000455"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFR1347024.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21626..22915
                     /gene="sdaC"
                     /locus_tag="ACHUT9_000456"
     CDS             21626..22915
                     /gene="sdaC"
                     /locus_tag="ACHUT9_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFR1347025.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22973..24340
                     /gene="sdaB"
                     /locus_tag="ACHUT9_000457"
     CDS             22973..24340
                     /gene="sdaB"
                     /locus_tag="ACHUT9_000457"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFR1347026.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24452..25207
                     /gene="xni"
                     /locus_tag="ACHUT9_000458"
     CDS             24452..25207
                     /gene="xni"
                     /locus_tag="ACHUT9_000458"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFR1347027.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25312..26460)
                     /gene="fucO"
                     /locus_tag="ACHUT9_000459"
     CDS             complement(25312..26460)
                     /gene="fucO"
                     /locus_tag="ACHUT9_000459"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFR1347028.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26488..27135)
                     /gene="fucA"
                     /locus_tag="ACHUT9_000460"
     CDS             complement(26488..27135)
                     /gene="fucA"
                     /locus_tag="ACHUT9_000460"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFR1347029.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27682..28998
                     /gene="fucP"
                     /locus_tag="ACHUT9_000461"
     CDS             27682..28998
                     /gene="fucP"
                     /locus_tag="ACHUT9_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFR1347030.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29031..30806
                     /gene="fucI"
                     /locus_tag="ACHUT9_000462"
     CDS             29031..30806
                     /gene="fucI"
                     /locus_tag="ACHUT9_000462"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFR1347031.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGSVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30915..32333
                     /gene="fucK"
                     /locus_tag="ACHUT9_000463"
     CDS             30915..32333
                     /gene="fucK"
                     /locus_tag="ACHUT9_000463"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFR1347032.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32335..32757
                     /gene="fucU"
                     /locus_tag="ACHUT9_000464"
     CDS             32335..32757
                     /gene="fucU"
                     /locus_tag="ACHUT9_000464"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFR1347033.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32815..33546
                     /gene="fucR"
                     /locus_tag="ACHUT9_000465"
     CDS             32815..33546
                     /gene="fucR"
                     /locus_tag="ACHUT9_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFR1347034.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33590..34690)
                     /gene="rlmM"
                     /locus_tag="ACHUT9_000466"
     CDS             complement(33590..34690)
                     /gene="rlmM"
                     /locus_tag="ACHUT9_000466"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFR1347035.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34683..35078)
                     /locus_tag="ACHUT9_000467"
     CDS             complement(34683..35078)
                     /locus_tag="ACHUT9_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFR1347036.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKCKGVSHE"
     gene            complement(35097..36014)
                     /gene="gcvA"
                     /locus_tag="ACHUT9_000468"
     CDS             complement(35097..36014)
                     /gene="gcvA"
                     /locus_tag="ACHUT9_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFR1347037.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36365..36592)
                     /locus_tag="ACHUT9_000469"
     CDS             complement(36365..36592)
                     /locus_tag="ACHUT9_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFR1347038.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36784..37989
                     /gene="csdA"
                     /locus_tag="ACHUT9_000470"
     CDS             36784..37989
                     /gene="csdA"
                     /locus_tag="ACHUT9_000470"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFR1347039.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37989..38432
                     /gene="csdE"
                     /locus_tag="ACHUT9_000471"
     CDS             37989..38432
                     /gene="csdE"
                     /locus_tag="ACHUT9_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFR1347040.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38483..39289)
                     /gene="tcdA"
                     /locus_tag="ACHUT9_000472"
     CDS             complement(38483..39289)
                     /gene="tcdA"
                     /locus_tag="ACHUT9_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFR1347041.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39366..40463)
                     /gene="mltA"
                     /locus_tag="ACHUT9_000473"
     CDS             complement(39366..40463)
                     /gene="mltA"
                     /locus_tag="ACHUT9_000473"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFR1347042.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40672..40748
                     /locus_tag="ACHUT9_000474"
     tRNA            40672..40748
                     /locus_tag="ACHUT9_000474"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40706..40708,aa:Met,seq:cat)
     gene            complement(40823..42076)
                     /gene="amiC"
                     /locus_tag="ACHUT9_000475"
     CDS             complement(40823..42076)
                     /gene="amiC"
                     /locus_tag="ACHUT9_000475"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFR1347043.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42308..43639
                     /gene="argA"
                     /locus_tag="ACHUT9_000476"
     CDS             42308..43639
                     /gene="argA"
                     /locus_tag="ACHUT9_000476"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFR1347044.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43701..45527)
                     /gene="recD"
                     /locus_tag="ACHUT9_000477"
     CDS             complement(43701..45527)
                     /gene="recD"
                     /locus_tag="ACHUT9_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFR1347045.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45527..49069)
                     /gene="recB"
                     /locus_tag="ACHUT9_000478"
     CDS             complement(45527..49069)
                     /gene="recB"
                     /locus_tag="ACHUT9_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFR1347046.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49062..51950)
                     /gene="ptrA"
                     /locus_tag="ACHUT9_000479"
     CDS             complement(49062..51950)
                     /gene="ptrA"
                     /locus_tag="ACHUT9_000479"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFR1347047.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYPQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52126..55494)
                     /gene="recC"
                     /locus_tag="ACHUT9_000480"
     CDS             complement(52126..55494)
                     /gene="recC"
                     /locus_tag="ACHUT9_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFR1347048.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55507..55830)
                     /locus_tag="ACHUT9_000481"
     CDS             complement(55507..55830)
                     /locus_tag="ACHUT9_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFR1347049.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55815..56222)
                     /locus_tag="ACHUT9_000482"
     CDS             complement(55815..56222)
                     /locus_tag="ACHUT9_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFR1347050.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56219..56782)
                     /locus_tag="ACHUT9_000483"
     CDS             complement(56219..56782)
                     /locus_tag="ACHUT9_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFR1347051.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56773..57243)
                     /locus_tag="ACHUT9_000484"
                     /pseudo
     CDS             complement(56773..57243)
                     /locus_tag="ACHUT9_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(57428..58222)
                     /gene="thyA"
                     /locus_tag="ACHUT9_000485"
     CDS             complement(57428..58222)
                     /gene="thyA"
                     /locus_tag="ACHUT9_000485"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFR1347052.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58229..59104)
                     /gene="lgt"
                     /locus_tag="ACHUT9_000486"
     CDS             complement(58229..59104)
                     /gene="lgt"
                     /locus_tag="ACHUT9_000486"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFR1347053.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59255..61501)
                     /gene="ptsP"
                     /locus_tag="ACHUT9_000487"
     CDS             complement(59255..61501)
                     /gene="ptsP"
                     /locus_tag="ACHUT9_000487"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1347054.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61514..62044)
                     /gene="rppH"
                     /locus_tag="ACHUT9_000488"
     CDS             complement(61514..62044)
                     /gene="rppH"
                     /locus_tag="ACHUT9_000488"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFR1347055.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            complement(62397..62543)
                     /gene="ygdT"
                     /locus_tag="ACHUT9_000489"
     CDS             complement(62397..62543)
                     /gene="ygdT"
                     /locus_tag="ACHUT9_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFR1347056.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            62729..63418
                     /gene="mutH"
                     /locus_tag="ACHUT9_000490"
     CDS             62729..63418
                     /gene="mutH"
                     /locus_tag="ACHUT9_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFR1347057.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63487..64200
                     /locus_tag="ACHUT9_000491"
     CDS             63487..64200
                     /locus_tag="ACHUT9_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFR1347058.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64338..64556
                     /gene="ygdR"
                     /locus_tag="ACHUT9_000492"
     CDS             64338..64556
                     /gene="ygdR"
                     /locus_tag="ACHUT9_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFR1347059.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15654 a        17367 c        15827 g        15749 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ctggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttag
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 aaagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaggcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggatttgg agccggagct gctggaactg
    11161 ttggacgaga tggataatgt cgcgcgcgaa gccagcaaaa ttctcgggta acggacgtct
    11221 tgtcgagtag caatcggctt tttatgacgc tggtttcaga ttggtgacaa agtgcctgtg
    11281 tttatgccgg atgcggcgtt aacgccttat ccggcctaca aaaccaatta aattcaatga
    11341 attaaaaaat gatgtagacc tgatacatag cacaacaggc tattttgcgt ttagcatcag
    11401 tctcaagccg gctccagata gagccggttt tggttttctg tcttaacgca ccatgcacgg
    11461 gcgcttgtta tcgaacgtcc agccaggaat cagatactgc attcccatcg catcgtcacg
    11521 cgcgccaagc ccgtgtttct gatacagctc atgggctttc atcacttgat ccatatcgat
    11581 ttctacaccc agccccggtt tttccggcac ctgaaccaga ccgcctttga tctcaaacgg
    11641 ttctttggtc aagcgctgat tgccttcctg ccaaatccag tgcgtatcaa tcgcggtgat
    11701 tttacccggt gcagcggcgg caacatgggt aaacatcgcc agggaaatat cgaagtggtt
    11761 gtttgagtgt gaaccccagg tcaggccaaa ttcatggcac atttgcgcca cacgtaccga
    11821 accttgcata gtccagaaat gcggatccgc cagcgggata tcaacggatt gtaacgagag
    11881 cgtatggccc atctgccgcc agtcggtggc gatcatattg gttgcagtcg gcaggccagt
    11941 cgcgcgacgg aactctgcca tcacttcacg cccggagaaa ccttgctccg caccatacgg
    12001 atcttctgca taagccagcg aacctttcag gtatttacca attttaattg cttcgttcag
    12061 cgaccaggca ccgttaggat cgagcgtaat acgcgcctgc gggaagcgtt gcgccagtgc
    12121 cacaatagac tcggcttctt cttccccggc cagtacaccg cctttcagtt tgaaatcgtt
    12181 gaagccatat ttttcatacg ccgcttccgc taggcgcacc accgcatccg gcgtcatcgc
    12241 ttcttcatga cgcaggcgat accagtcgca tgagtcatcc ggctggcttt gatacggcag
    12301 aggtgtggct ttgcgattac cgacgaagaa cagataaccg agcatttcga cttcgctacg
    12361 ctgttgacca tcgcccagca gcgatgccac gtttaccccc agatgctgcc ccagcagatc
    12421 cagcattgcc gcttctatcc cggtaactac atgaatagtg gtacgcaaat caaatgtctg
    12481 caaaccgcgc ccaccagcat cacgatcggc aaaagtatta cgcaccagcg tcagaacgtt
    12541 tttgtattca cccagcgttt tacctaccac cagcggaatc gcatcttcca gcgttttacg
    12601 gattttctcg ccgccgggaa tttcccctac gccagtgtga ccggaattat ctttgataat
    12661 cacaatatta cgcgtaaaga acggtgcgtg tgccccactc agattcatca gcatactgtc
    12721 atgacccgcc accgggatga cctgcatttc agtaacaaca gacgtcgtaa attgagaact
    12781 catatttatg tcctttttca gaatcaatga cggccgaaaa cgggacgttt acggtcaaag
    12841 gtccagccgg ggatcaggta ctgcatcgga cctgcgtcgt tacgcgcacc gccaggcaga
    12901 cgtttatagg cctcatgtgc cttttgtacc tgttcccagt ccagttccac gcccagaccg
    12961 ggcgcatcag gaacggcaat ttttccgttt ttaatctcca gcggattttt ggtcaggcga
    13021 caatcgccct cctgccaaat ccagtgggta tcgatagcgg taggattacc cggtgccgcc
    13081 gcgcccacat gggtaaacat cgccagagag atatcgaaat ggttattaga atggcagccc
    13141 caggtcagcc cccagtcgtc gcaaagctgc gccacacgga ctgcaccgga aagcgtccag
    13201 aagtgcggat cggcaagtgg aatatctacc gcattgagca tcaccgcatg acccatttcg
    13261 cgccagttgg tggcgatcat gttagtcgcg acgggcaagc cggtcgcccg ccgaaactcc
    13321 gccataactt cacgaccgga gaaaccctgt tctgcgccgc atggatcttc ggcataggta
    13381 agaacatcat tcagcccttt gcatagagaa atggcttcat caagtagcca ggcaccgttg
    13441 ggatcaacgg taatccgcgc atcagggaag cgtttcttca atgcacgaac agtgtcgatt
    13501 tcttgctcgc caggtaacac cccgccctta agtttgaaat ctttaaagcc atagcgatcc
    13561 tgtgaggctt ccgccagacg cacaacggct tcgctgttca tcgctttctg atggcgcaac
    13621 tgataccact catggttgcc cggcgtattt tccagataag gaagatcggt tttggtccga
    13681 tcaccgatat aaaacagata accgaggacg gtaatagtct cgcgttgctt gcctggcccc
    13741 aacagttcgc agaccggaac attcagcgcc ttacctagca ggtcaagcaa ggcggcttcc
    13801 agcgccgcca cggcattaac gcgcaattca aaagtccagg cacctttgcc gaaggtatca
    13861 aaatcggctg cctgattacc tttatgcacc tgctgaacca ctttattcag gcgcgcaact
    13921 tcctggccca gaaccatcgg aatagcatcg accagtgttt gataaatcac ctctccgccc
    13981 ggcgcttcac caatgccggt atgcccggcg ttatcggtga gtaccacaat attgcgggtg
    14041 aaatatgcgt tatgtgcccc accaatatta agcaacatgc tatcgtgccc ggcgaccgga
    14101 atgactttca tatcagtaat aacaggactg gattgtgtcg tcatcttatt gccccgcgac
    14161 aggtttcagc tcgatacgct tgatatcgcc caccagcacc aggtagctca gtaccgcgat
    14221 taaggcatga acaccaacat aaatcagcgc cccattaaag gagccagtcg tgccaacgat
    14281 ataaccaatt gcgattggcg tgacgatgcc agaaatgtta ccgaacatgt tgaacaggcc
    14341 accggaaaga ccgctgatct cttttggcgc ggtatctgcc attactgccc aacccagcgc
    14401 cccgatgccc ttaccgaaga aggccagcgc cataaagccg atgatcatcc actcaacgtt
    14461 gacgtagttg cagaacacca tcaccatcga cagcaacatg cccattacga tcggtgtttt
    14521 acgcgcaatg ttcagcgatc ccgtgcgacg catcagccag tcggaaataa tcccacccag
    14581 cacaccgccg ataaaaccgc aaaccgccgg aacggaagcc acaaagcccg ctttcagaat
    14641 cgacatccca cgcgcctgca ccagataaac cggaaaccag gtaatgaaaa agtaagtcag
    14701 ggcgttgata cagtactgac cgatataaac gccgatcatc atccgtgacc cgagcagttg
    14761 tttgatctgc ccccacttca cgctgaacgg cactttaact ttggtgtttt gctgatccat
    14821 attgatcagc gcgccacccg cggcgatgta ctccagctct ttctgattta cccccggatg
    14881 ttgatttggc tcgtggatga ctttcaacca gataaagctg atgacaatcc ccagaccgcc
    14941 cataaagaag aagacgtgtg accagcccac ttcatgcgtc agccagccca taatcggcgc
    15001 gaagatcacc gttgcgaagt attgagcgga gttaaaaatc gacaccgccg ttcccctttc
    15061 ctgcgccgga aaccaggccg caacaatgcg actgttgccg gggaaagatg gcgcttcagc
    15121 aagcccgacc aggaagcgca gagtaaacag ggcaacgata atgccgaatc cactaaagat
    15181 atcgacgaag ccttgcagca aggtaaacat cgaccagata aagatcgacc agaagtagac
    15241 gcgttttgaa ccaaaacgat ccagcaacca accaccaggg atctgcccga taacataagc
    15301 ccatgagaag gcagagaaca catagcccat tcccacggga tcaaggccga tatctttggc
    15361 catttccgaa ccggcaatag aaagcgtagc gcggtcgccg tagttgaagg atgtgacgat
    15421 aaacaacatc accactatcc agtaacgagc attcgtgcgt ttttccacac tgctcgcagc
    15481 ctgacttaaa gaactcattg ttgcactcct gaaaattcgc gttagccacg ctcactctgg
    15541 actgcgacat agccaggaaa tcagaggtga cgtagggtgt tttttgccgt ttttataggt
    15601 cgttcgccga atacggcgcg tgtttatatc tggcaatagc agtataaaaa gcgcgccata
    15661 gcggctcacc gtgcaacaac acaacattaa tgcgttcaat gaggcccgat tttggcatta
    15721 gccctggacg gtggaatcca cttcacggaa atgaaaacaa gaacaagaaa ggaagggtta
    15781 aaacgaagaa ataaagaaga gtatgaaatg gatcgcttga ctccaggcaa acgccagtaa
    15841 aaatccgcgc tatgaagcag tttttactgg catttgcctg aaaagattcg attcagcacc
    15901 gctaaaacga catttaccgc tcgctgaaca tatcaggaca acagcgtgcc ccactgttcg
    15961 acccacggat ttgattccgt ttccggttcc gggttctcac tggcgtcaat caacagcatt
    16021 tcgccaaccc gctgagcgct ctgttcctgc aacaaggcat cgaactgttt gccgccattg
    16081 cagaaattta cataactgct gtcgccgagc gcaatcacgc cataacgcag attcggctgg
    16141 aagcccagac tatctttgat tccctgaaag agcggcacaa tgctatcagg aaggtcgccc
    16201 tgcccggtcg tggacgtaac caccagaaca tacttatcct gataaggcag ccagtcgctt
    16261 aattcaggat cttcaaatac cgttgctttg tggccctgtg cggtcagaat tgcttctgct
    16321 tcttccgcca ctaacagcga atttccgtac atggtgccga caaaaatacc aatttccgcc
    16381 ataccgtttt ctccctggat taggaactta tctctccatc ctgacccgat ggcgcactga
    16441 actcaaccct ttcattttca ggaagcagac cgcgccagcc aaattgtgat aacgcctgca
    16501 tccaggtgtc gtccaggccc gcgtgaatag tcagcggctc gccagtaaaa ggatgcgtca
    16561 atgacaactg actggcatgt agcattaacc gctggaggcc aaaatgctca gcaccgctgc
    16621 gattctggcg taaatcgcca tgtttgctat cgccaataat cggatgacgc aaatgggcaa
    16681 gatgtcggcg aagctgatgt ttgcgtccgg ttttcggctc cagttccacc aggccgtagc
    16741 gcgtggtcgg gtaacgtccg gtcgctaccg gcatttctac ggtcgccaga ccgcgataat
    16801 gcgtcactgc tggctgcggg cctttatctt cgcgggcaaa tttatcagcg attttgtcca
    16861 gttcttccac cagtggataa tccagcaccg cttcttccat caaccagccg cgcacaatcg
    16921 catggtaacg tttctggatt tggtgctgtt caaactgttg tgccagcagc cgtccggcct
    16981 cgctggataa tcccatcaac aacacaccag aagtgggtcg gtccagacga tgagcagtaa
    17041 aaacatgctg tcctatctgg tcacgcacgg tttgcatgac cactactttc tcgtcgcgat
    17101 ccagccagct acggtgaacc agccagccgg agggtttatt taccgcaacc agccattcat
    17161 cctgatagag tatttccagc attagctcgc atcatccgca aaaagagcat ccagtttttc
    17221 caactcagcc agaataagcg cgcgttgcgg atggtccgtc gccagcgcca tttcataata
    17281 gggtgcaaca gcaaaagcgc ccggtaacgg ctggttgtta tttaacaaat cgtgcattcg
    17341 cgggatcagc acccactgca accactccag tggttccatg gtgtccataa agaacggttg
    17401 ggtactatta aattgatgcg gctggggttc atcgtttcgc cagtgctgat gttcacgcag
    17461 taacgcttca agcgtctgca actggaaacg aacgcggtca tgagtggtca taaagcaact
    17521 tcaataagaa aaactgccgc gaaggatagc aggaataaaa aaagggagca ctgtattcac
    17581 agtgctcccg gttcgtttcg cagcattcca gctacttttg ttgctccctg ctcatccttg
    17641 acaacttttc ctctggcctt gcggccaatc gttcatcctg aactattgct tcctgctcac
    17701 accaccccga tgtgatactt catcctgaag tgtccctggc cttcctgacc caccgaatca
    17761 tcctgaccgg ttctcattct ccatcctgga ggtgtccttt aacgcgtcct gcgtcatcct
    17821 cttcgcttca tccagaagcc tttccctgaa acaccatcct ggtgtgtcct gcagaagtgt
    17881 catcatcctg atgttcactt cgttgtctga ctccctgtcg acgaagatag aatcgtcttt
    17941 ttcgcgaagt cttacaaggc gcttacagac aatgccttaa ggaaaatttc atatgaaagt
    18001 taagaattaa caatcaacat tttgatttta aatgaaaata acgattctta tctttttaag
    18061 tattcgctta tttcctggcg atctcctaca gggtctgtaa gagatctctc acaaccagat
    18121 ggggtatttg gattacagaa gaggcttaag ttgattaagg aattccgcaa gatttgaggc
    18181 taaatgcgtc cgtttacgtg ttcccagcgt ctctttgcac acttcaccgg agagattaca
    18241 caccgagatg acctctaact cttcttccag cgtcgcgata aatagcgtgg gcggcagttt
    18301 tagccttttc tgagtcacca gatgaccaat cagattttcc tgcacccgac ggaagtcgtc
    18361 ttcactccag gtttgcagca atgtcagttt gatatcgcca aactgcgcgt gcatatcccc
    18421 ggcaaactga gtcgtataaa aggtatgaat tgtgggttgt atcacaatat caaaagcgcg
    18481 ttcaaccgcg tttacatttt gttccccggt aaacggctgg ggttgccagt atacggcatc
    18541 ttcggtagtt gaaataatgc acggtgaagg aacaccgtac agttcctcac ttaacggcca
    18601 gcttttatgc tcttcatgcc acgcatcgca gtagcgtgca gtaaaatctt tcagggcttg
    18661 tgcggtcaaa tcgtccacta atttctctct tcacttaaac cagatacact tgtcgtttag
    18721 tttatctggt ttatgacggt gaaacatgtc ttcttatgca aaccatcagg cacttgcggg
    18781 cctgactctt ggaaaatcaa ccgattaccg ggatacctat gacgccagcc tactgcaagg
    18841 cgttccacgc agcctgaatc gcgacccgct gggtctgaaa gcggataacc tgccttttca
    18901 cggtacggat atctggacgc tgtatgaact ttcctggctg aatgcgaaag gtttgccgca
    18961 ggtcgctgtc ggtcatgttg aacttgatta caccagcgta aatctgattg agtcgaagag
    19021 ttttaagctc tatctcaaca gttttaacca gacgcgtttt aataactggg atgaggtgcg
    19081 ccagacgctg gagcgcgact taagcacttg cgctcagggt aagattagcg tggcgttata
    19141 tcgtcttgat gaactggaag gccagccgat aggtcatttt aatggcactt gcattgatga
    19201 ccaggatatc actatcgata actatgaatt cactactgac tatctggaga atgccacctg
    19261 tggtgaaaaa gtagtggaag agacgcttgt cagccacctg ctgaaatcaa actgcctgat
    19321 cacccatcaa ccagattggg gttcgatcca aattcagtat cgtggacgcc aaattgacag
    19381 agaaaaactg ctgcgttacc tggtctcatt ccgtcatcac aacgagttcc acgaacagtg
    19441 cgtggaacgc atctttaatg acctgttacg cttctgccag ccagaaaaat tgagcgttta
    19501 cgcacgttat acccgtcgtg gcggtctgga cattaacccg tggcgcagta atagcgattt
    19561 tgtcccatcg accacaagac tggttcggca ataaattttt tctcaatttt gcgtgctgga
    19621 ttcacgcaga aggttgtgaa aggacatcag gcagggctat tgtaatcaaa gggaatgacg
    19681 atattcgtcc cataaggagt ttttcttgat tacacatatt agcccgcttg gctccatgga
    19741 tatgttgtcg cagctggaag tggatatgct taaacgcacc gccagcagcg acctctatca
    19801 actgtttcgc aactgttcac ttgccgtact gaactccggt agtttgaccg ataacagcaa
    19861 agaattgctg tctcgttttg aaaatttcga tattaacgtc ctgcgccgtg aacgcggcgt
    19921 aaagctggaa ctgattaatc ccccggaaga ggcttttgtc gatgggcgaa ttattcgcgc
    19981 gttgcaggcc aacttgttcg cggttctgcg tgacattctc ttcgtttacg ggcaaatcca
    20041 taacaccgtt cgttttccca acctgaatct cgacaactcc gtccacatca ctaacctggt
    20101 cttttccatc ttgcgtaacg ctcgcgcact gcatgtcggt gaagcgccaa atatggtggt
    20161 ctgctggggc ggacactcaa ttaacgaaaa cgagtatttg tatgcccgtc gcgtcgggaa
    20221 tcagctgggc ctgcgtgagc tgaatatctg caccggctgt ggtccgggag cgatggaagc
    20281 gccgatgaaa ggtgctgcgg tcggacacgc acagcagcgt tacaaagaca gtcgttttat
    20341 tggtatgaca gagccgtcga ttatcgccgc tgaaccgcct aacccgctgg tcaacgaatt
    20401 gatcatcatg ccagatatcg aaaaacgtct ggaagcgttt gtccgtatcg ctcacggcat
    20461 cattatcttc cctggcggtg tgggtacggc agaagagttg ctgtatttgc tgggaatttt
    20521 aatgaacccg gccaacaaag atcaggtttt accattgttc ctcaccggcc cgaaagagag
    20581 cgccgactac ttccgcgtac tggacgagtt tgtcgtgcat acgttgggtg aaaacgcgcg
    20641 ccgccattac cgcatcatca ttgatgacgc cgctgaagtc gctcgtcaga tgaaaaaatc
    20701 gatgccgctg gtgaaagaaa atcgccgcga cacaggcgat gcctacagct ttaactggtc
    20761 aatgcgcatt gcgccagatt tgcaaatgcc gtttgagccg tctcacgaga atatggctaa
    20821 tctgaagctt tacccggatc aacctgttga agtgctggct gccgatctgc gccgtgcgtt
    20881 ctccggtatt gtggcgggta acgtaaaaga agtcggtatt cgcgccattg aagagtttgg
    20941 tccttacaaa atcaacggcg ataaagagat tatgcgtcgt atggacgacc tgctacaggg
    21001 ttttgttgcc cagcatcgta tgaagttgcc aggctcagcc tacatccctt gctacgaaat
    21061 ctgcacgtaa tctccctccc ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac
    21121 atacccatca atttatttca tttgttatat gaatgtttct taccaccctc acgcgataaa
    21181 tatcattgca gttaatatgt catacaattt atgttgtaac gcaaacgttt ccctattttc
    21241 ataaaccaca ttttatcgct atgaaaaaga aattatcgcc atgattaact aaaagtattg
    21301 attttttcag ttcaacctac atatattgcg cgccccggaa gaagtcaaat gtcgtttaat
    21361 gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa gcgttgggat
    21421 aatctatctt ccaagtagat tattgtattt gggatcaaga tcactgatag atacataact
    21481 tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc atcgccgata
    21541 gacagatttc atttttacgg tcaggcacct tcccgagctg aactggctaa aagctgaatt
    21601 atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga ttgcgtcgaa
    21661 agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc tttacggcac
    21721 ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc
    21781 gctgatcatc atggctatcc ttgcgttccc gatgacattt tttgctcacc gcggcctgac
    21841 tcgcttcgta ctgtctggta aaaacccagg cgaagacatc accgaggttg tagaagaaca
    21901 ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta tctacccgat
    21961 cctgctggtt tatagcgtgg caatcaccaa tacagttgaa agcttcatgt ctcaccagct
    22021 gggtatgaca ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac
    22081 catcgttcgc ttcggtgagc agatgatcgt taaagcaatg agtattctgg tattcccgtt
    22141 tgttggcgta ctgatgctgc tggctctgta cctgatcccg cagtggaacg gcgcagcact
    22201 ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg
    22261 gctggcaatt ccggttatgg tgttctcttt caaccactct ccgatcatct cttctttcgc
    22321 cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga agatcctggc
    22381 attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct gtgtactgag
    22441 cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc tgtcttacct
    22501 ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg cgattatcgc
    22561 tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca acggtatggt
    22621 gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga accgtatcac
    22681 tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga gcatcctggg
    22741 tatgattgaa accctgggcg gcccaatcat cgcgatgatc ctgttcctga tgccgatgta
    22801 cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca acgtattcgt
    22861 tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca gctaagtcct
    22921 ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc ctatgattag
    22981 cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg ttggaccaat
    23041 gaaagcgggt aaacaattta ccgacgatct gattgcccgt aatctgctta aagacgtgac
    23101 ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc accacactga
    23161 tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata tcgattccat
    23221 ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa acggtcagca
    23281 tgaagtggag ttcccggttg atcagtgcat gaacttccat gcagacaacc tttctctgca
    23341 tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca gccagactta
    23401 ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc agaatagcgc
    23461 accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac attgtcagga
    23521 aaccgggctg tcactctctg gcctgatgat gaaaaatgag ctggcgctgc acagcaaaga
    23581 agagctggaa cagcacctgg cgaacgtatg ggaagtcatg cgtggcggta ttgagcgcgg
    23641 tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact
    23701 acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg ttgttgactg
    23761 gatcaacatg tttgcactgg cagtgaacga agagaacgct gctggcggac gcgtggtgac
    23821 tgcgccgact aacggtgcgt gcgggattat cccggcagtg ctggcgtact acgacaagtt
    23881 catccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca gtgccattgg
    23941 ttctctttat aagatgaacg cgtcaatttc tggtgctgaa gtcggctgcc agggtgaagt
    24001 tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa ctgttaggtg caagcccggc
    24061 gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga cgtgtgaccc
    24121 ggtcgccgga caggtccagg tgccatgcat cgagcgtaac gccattgcgg cagtaaaagc
    24181 ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag ccgcgcgtct gcctcgataa
    24241 agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc gcgaaacctc
    24301 tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca gaggcctcgt
    24361 tttgcgaggc ctcttcccga tttctcatcc agccgtagcc tgttccggca tcgaatgtta
    24421 cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta ttgtcgatgc
    24481 actgaatctt attcgtcgca ttcatgccgt tcagggatcg ccctgtgtcg aaacctgcca
    24541 gcatgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg ccgtttttga
    24601 tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca aagcgggtcg
    24661 accgccaatg ccggaagagt tgcacgacga gatgcctgca ttacgcgccg cctttgagca
    24721 acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc gatgacttag ccgccacgct
    24781 ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag ataaaggcta
    24841 ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac gttggctgga
    24901 tgcgccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg attactgggg
    24961 acttgcgggg atcagcagtt caaaggtacc gggtgttgcg ggaatcggac caaaaagcgc
    25021 cacgcagctg ctggtcgagt ttcagagtct ggaagggata tatgagaatc tggatgcggt
    25081 tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc tgtgccgcga
    25141 tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat tgcggttggt
    25201 acggtaacgg cgagccggat gcaaacgatt atcaacacat tgaatttaat gcacttatat
    25261 accggataag acgcggctag cgtcgtatcc ggcattatca catcagcgca tttaccaggc
    25321 ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc caccggtaca
    25381 aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta caccaacatc
    25441 acgcaaatgt ggcggaatac cgacatcacg gttgagagca aacaccgctt caacagcggc
    25501 attacgcgcc tcttccaggc tcataccttc cactttcacg cccataacgc gcgcgatatc
    25561 gcggtacttc tcaccggtaa agtcagcgtt atagcgcatg acatgcggta acaggatggc
    25621 gttcgcaaca ccgtgtggag tgttgtaaaa cgcgcccagt ggatgcgcca taccatgcac
    25681 caaccctaac ccaacattcg agaagcccat acccgcaaca tactgcccga gcgccatttc
    25741 ttctccggca tccttatcac cagcaaccga tcctcgcagc gccccagcaa tgatttcaat
    25801 cgctttaatg tgcagtgcat cggttagcgc ccacgcgcca cgggtaatat acccctcaat
    25861 agcatgagtg agcgcatcga cacccgtcgc agctttcagc gctggaggca taccatccat
    25921 catgtcagcg tcaataaacg ccacctgcgg gatatcatgc ggatcaacgc aaacaaactt
    25981 gcgccgtttc tcttcgtcag tgatcacgta gttaatggtc acttctgccg cagtacctgc
    26041 tgtggtagga attgccagaa tcggtacact gggtttattg gtcggggaaa gcccttccag
    26101 gctacgcaca tcggcaaact ccgggttgtt gctgataatg ccaatcgctt tacaagtatc
    26161 ctgtggagaa ccaccaccaa tagcgatcag gtaatccgcg ccgctattct ggaatacacc
    26221 gagcccttct ttgacgacag taattgttgg gttgggcact acgccgtcgt aaatcgccca
    26281 tgccagccct gcagcatcca tcttatcggt cactttcgcc accacgccgc attgcaccag
    26341 cgttttatcg gtgacgatca gcgccttctg ataaccacgg cgtttcacct catcggttaa
    26401 agccccaaca gcaccccgac caaaccatgc cgtttcgttc agaatcattc tgttagccat
    26461 catccttctc cttgttgctt tacgaaatta ctcttcaatc cgtaacccat aggttttgaa
    26521 tttctccagc actacggcaa tctcttcatc gctcagcact ggcaccgggt ccgtaatcgc
    26581 cagggtcgtc acgtaaagtt gcgccagcac ttcaacttca tgcgccagcc ataacgcttt
    26641 ttccagattc acctcacaag cgataagccc atgatgttgt aacaaagttg ccttacgatt
    26701 tttgagagcc agcgcaacat gttcagaaag ttcgcgtgtt ccaaaggtcg cataaggcgc
    26761 gcaaggaata gaattaccac ctgccgccgc aatcatgtag tgaatagcgg ggatcggtcg
    26821 gttaagaatg gaaactgctg tgcaatgaac ggcatgattg tgaacaaccg cgttggcatc
    26881 cggtctgctt tgataggccg ccatatggaa acgccattcg cttgagggga gctttccttc
    26941 ctcatgttta ccgttgccat caataaagac aatatgcgac tccgtcagtt tttcatatgg
    27001 aatgcctgta ggcgtaatca gcatcccatc ctgataacgt acactgacgt tccccgctgt
    27061 cccctggttc agtcccaggc gggtcatttc caggcaagtg tcaataatct gacgagcaag
    27121 tttatttcgt tccattagct acctctctct gattcaaaac agggcaataa tgttgttcct
    27181 ttcacactat tgaattagcc gtttaattac ccaccatctt cttcctgatt aacaagaaag
    27241 aaattcacaa gcttatattt tgtgacctag ctcaactaat cacagtaaat aactgcaagt
    27301 tctcttttta taaccccatt aaaaatgacc gctcttaaaa atatttatca aaacggtcat
    27361 ttttctattc acccaagcca ggaatgcccg ttttcggcat aaacaattaa tacagtcatc
    27421 tggtttgtgt tttttatgat ttattttctg aaacgggcat gaaatttcga ttattaaagt
    27481 gatggtagtc acataaagtc accttccagc taataagtgt gaccgccgtc atattacaga
    27541 gcgtttttta tttgaaaatg aatccatgag ttcatttcag acaggcaaat attcactgat
    27601 atgaagcccg aactcgctgg ttttgcactt ttgaaaacat aaccgattac gtgcttaagc
    27661 ttctgaacct aagaggatgc tatgggaaac acatcaatac aaacgcagag ttaccgtgcg
    27721 gtagataaag atgcagggca aagcagaagt tacattattc cattcgcgct gctgtgctca
    27781 ctgttttttc tttgggcggt agccaataac cttaacgaca ttttattacc tcaattccag
    27841 caggctttta cgctgacaaa tttccaggct ggcctgatcc aatcggcctt ttactttggt
    27901 tatttcatta tcccaattcc tgctgggata ttgatgaaaa aactcagtta taaagcagga
    27961 attattactg ggttattttt atatgccctg ggcgctgcat tattctggcc cgctgcagaa
    28021 ataatgaact acaccttatt tttagttggc ctatttatta ttgcagccgg attaggttgt
    28081 ctggaaactg ccgcaaaccc ttttgttacg gtattagggc cggaaagcag tggtcacttc
    28141 cgcttaaatc ttgcgcaaac atttaactcg tttggcgcaa ttatcgcggt tgtctttggg
    28201 caaagtctta ttttgtctaa cgtgccacat caatcgcaag acgttctcga taaaatgtct
    28261 ccagagcaat tgagtgcgta taaacacagc ctggtattat cggtacagac accttatatg
    28321 atcatcgtgg ctatcgtgtt actggtcgcc ctgctgatca tgctgacgaa attcccggca
    28381 ttgcagagtg ataatcacag tgacgccaaa caaggatcgt tctccgcatc gctttctcgc
    28441 ctagcgcgta ttcgccactg gcgctgggca gtattagcgc aattctgcta cgttggcgca
    28501 caaacggcct gctggagcta tttgattcgc tacgctgtag aagaaattct aggtatgact
    28561 gcaggctttg ccgctaacta tttaaccgga accatggtgt gcttctttat tggtcgtttc
    28621 accggtacct ggctcatcag tcgcttcgca ccacacaaag tcctggccgc ctacgcatta
    28681 atcgctatgg cactgtgcct gatctcagcc ttcgctggcg gtcatgtggg cttaatagcc
    28741 ctgactttat gcagcgcctt tatgtcgatt cagtacccaa caatcttctc gctgggcatt
    28801 aagaatctcg gccaggacac caaatacggt tcgtccttca tcgttatgac cattattggc
    28861 ggcggtattg tcactccggt catgggtttt gtcagtgacg cggcgggcaa catccccact
    28921 gctgaactga tccccacact ctgcttcgcg gtcatcttta tctttgcccg tttccgctct
    28981 caaacggcaa ctaactgaac atattttccg aataaagtga ggaatctgta atgaaaaaaa
    29041 tcagcttacc gaaaattggt atccgcccgg ttattgacgg tcgtcgcatg ggtgttcgtg
    29101 agtcgcttga agaacaaaca atgaatatgg cgaaagctac ggccgcactg ctgaccgaga
    29161 aactgcgcca tgcctgcgga gctgccgtcg agtgtgtcat ttccgatacc tgtatcgcgg
    29221 gtatggctga agccgctgct tgcgaagaaa aattcagcag ccagaatgta ggcctcacca
    29281 ttacggtaac gccttgctgg tgctatggca gtgaaaccat cgacatggat ccaacccgcc
    29341 cgaaagccat ttggggcttt aacggcactg aacgtcccgg cgctgtttac ctggcagcgg
    29401 ctctggcagc tcacagccag aaaggcatcc cggcattctc catttacggt catgacgttc
    29461 aggatgccga tgacacatcg attcctgccg atgttgaaga aaaactgctg cgctttgccc
    29521 gcgccggttt ggccgtcgcc agcatgaaag gtaaaagcta tctgtcgctg ggcggcgttt
    29581 cgatgggtat cgccggttct attgttgatc acaacttctt tgaatcctgg ctgggaatga
    29641 aagtccaggc ggtggatatg accgaactgc gtcgccgtat cgatcagaag atttacgacg
    29701 aagccgaact ggaaatggca ctgacctggg ctgataaaaa cttccgctat ggcgaagatg
    29761 aaaataacaa acagtatcaa cgcaatgccg agcaaagccg cgcagttctg cgcgaaagtt
    29821 tactgatggc gatgtgtatc cgcgacatga tgcaaggcaa cagcaaactg gccgatattg
    29881 gtcgcgtgga agaatcactt ggctacaacg ccatcgctgc gggcttccag gggcaacgtc
    29941 actggaccga tcaatatccc aatggtgaca ccgccgaagc gatcctcaac agttcatttg
    30001 actggaatgg cgtgcgcgaa ccctttgtcg tggcgaccga aaacgacagt cttaacggcg
    30061 tggcaatgct aatgggtcac cagctcaccg gcaccgctca ggtatttgcc gatgtgcgta
    30121 cctactggtc accagaagca attgagcgtg taacggggca taaactcgat ggactggcag
    30181 aacacggcat catccatttg atcaactcag gttctgctgc gctggacggt tcctgtaaac
    30241 aacgcgacag cgaaggtaac ccgacgatga agccacactg ggaaatctct cagcaagagg
    30301 ctgacgcttg cctcgccgct accgaatggt gcccggcgat ccacgaatac ttccgtggcg
    30361 gcggttactc ttcccgcttc cttaccgaag gcggcgtccc gttcaccatg actcgtgtca
    30421 acatcatcaa aggcctggga tcggtactgc aaatcgcgga aggctggagc gtggaattgc
    30481 cgaaggatgt gcatgacatc ctcaacaaac gcaccaactc aacctggcca accacctggt
    30541 ttgcaccgcg cctcaccggt aaagggccgt ttacggatgt gtactcggta atggcgaact
    30601 ggggcgctaa ccatggggtt ctgaccatcg gccacgttgg cgcagacttt atcactctcg
    30661 cctccatgct gcgtatcccg gtatgtatgc acaacgttga agagaccaaa gtgtatcgtc
    30721 cttctgcctg ggctgcgcac ggcatggata ttgaaggcca ggattaccgc gcttgccaga
    30781 actacggtcc gttgtacaag cgttaataca ttactccctg atgtgatgcc cggtcgctcc
    30841 ggctaccggg cctgaacaag caagagtggt tagccggata agcaatgtta tccggctata
    30901 ttgcaggagc gattatgaaa caagaagtta tcctggtact cgactgtggc gcgaccaatg
    30961 tcagggccat cgcggttaat cggcagggaa aaattgttgc ccgcgcctca acgcctaatg
    31021 ccagcgatat cgcgatggaa aacaacacct ggcaccagtg gtctttagac gccattttgc
    31081 aacgctttgc tgattgctgt cggcaaatca atagtgaact gactgaatgc cacatccgcg
    31141 gtatcgccgt caccaccttt ggtgtggatg gcgctctggt agataagcaa ggcaatctgc
    31201 tctatccgat cattagctgg aaatgtccgc gaacagcagc ggttatggac aatattgaac
    31261 agttgatctc cgcacagcgg ttgcaggcta tttctggcgt cggagccttt agtttcaata
    31321 cgttatataa gttggtgtgg ttgaaagaaa atcatccaca actgctggaa cgcgcgcacg
    31381 cctggctctt tatttcgtcg ctgattaacc accgtttaac cggcgaattc actactgata
    31441 tcacgatggc cggaaccagc cagatgctgg atatccagca acgcgatttc agtccgcaaa
    31501 ttttacaagc caccggtatt ccacgccgac tcttccctcg tctggtggaa gcgggtgaac
    31561 agattggtac gctacagaac agcgccgcag caatgctcgg cttacccgtt ggcataccgg
    31621 tgatttccgc aggtcacgat acccagttcg ccctttttgg cgctggtgcc gaccaaaatg
    31681 aacccgtgct ctcttccggt acatgggaaa ttttaatggt ccgtagcgcc caggttgata
    31741 cttcgctgtt aagtcagtac gccgattcta cctgcgaact ggatagccag gcagggttgt
    31801 ataacccagg tatgcaatgg ctggcatccg gcgtgctgga atgggtgaga aaactgttct
    31861 ggacggctga aacaccctgg caaatgttga ttgaagaagc tcgtctgatc gcacctggcg
    31921 cggatggtgt gaaaatgcag tgtgatttat tgtcgtgtca gaacgctggc tggcaaggag
    31981 tgacgcttaa taccacgcgg gggcatttct atcgcgcggc gctggaaggg ttaactgcgc
    32041 aattacagcg caatctacag atgctggaaa aaatcgggca ctttaaagcc tctgaattat
    32101 tgttagtcgg tggaggaagt cgcaacacat tgtggaatca gattaaagcc aatatgcttg
    32161 atattccggt aaaagttctc gacgatgccg aaacgaccgt cgcaggagct gcgctgttcg
    32221 gttggtatgg cgtaggggaa tttaacagcc cggaagaagc ccgtgcacag attcattatc
    32281 agtaccgtta tttctacccg caaactgaac ctgaatttat agaggaagtg tgaaatgctg
    32341 aaaacaattt cgccgttaat ttctcccgaa ctattgaaag tgctggcaga gatgggacat
    32401 ggagatgaaa ttattttttc cgatgctcac tttcccgccc attcgatggg accgcaggtg
    32461 atccgcgctg atggcctgtt ggtgagcgac ttgctccaag cgattatccc attatttgag
    32521 ctggacagtt atgccccgcc gctggtgatg atggcagcgg tagaaggtga cactctcgat
    32581 cctgaagtag aacgacgtta ccgtaatgcg ctttcactac aagccccgtg tcctgacatc
    32641 atccgcatca atcgttttgc gttttatgaa cgggcgcaaa aagcctttgc gatcgttatc
    32701 acaggcgaac gagcgaagta cgggaatatt cttttaaaaa aaggggtaac accgtaatct
    32761 catatcggta cgcccgcatg acgcgggcgg ttatcgaatg atggggtgaa aaatatgaaa
    32821 gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc ataccagcct gaccacggaa
    32881 gctctctctg aacagctaaa ggtcagtaaa gaaaccattc gtcgcgatct caatgaatta
    32941 cagacgcagg gtaaaattct gcgcaatcat ggacgcgcta aatatatcca ccgtcaaaat
    33001 caagacagtg gcgatccctt tcacatcagg ctgaaaagcc attatgcgca taaagcagat
    33061 atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg tgatagcctt agacgccagt
    33121 tcaacttgct ggtatctggc acgccagttg cctgacatca acattcaggt cttcaccaat
    33181 agccatccga tttgccatga actcggtaaa cgcgaacgca ttcaactgat cagttccggc
    33241 ggcacacttg agcgcaaata tggctgttac gtcaatccct cgctgatttc ccaacttaaa
    33301 tcgctggaaa tcgatctgtt tattttttct tgtgaaggga tcgatagcag cggcgcactg
    33361 tgggactcca atgcgatcaa cgctgattac aaatcgatgc tattaaaacg tgccgcgcaa
    33421 tcgttgttat tgattgataa aagtaaattt aatcgttcag gggaagcccg catcgggcat
    33481 ctggatgagg taacgcacat tatttctgat gagcgccagg ttgcaacttc tttggtaaca
    33541 gcctgacgga aagggtagca ggccgaagac gacgccccgg ccttgcctgt tatcgctcgt
    33601 cgcgacgacc acccaccgca gcccagatgc ggcggacgtg caccgtcact tcttcgcgat
    33661 cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc atgttcatca agctgtgcct
    33721 gaatatacgc cagattgtgt gatacttctt cgtagcgttt tttcatcggc agtttgagat
    33781 tgaaaatggt ttcacggcac cagccattaa ccagccactg cgccatcaat gccgcaactt
    33841 tagccggttt ttcaaccata tcgcatacca tccaggagat attgctgcgt gtcggacgga
    33901 atttgaaacc gtcttcccgc agccacgtca cctgcccggt atccatcaga ctttgcgcca
    33961 tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt caccagttgg taggtccagc
    34021 cgccagggca agcgcctaaa tccaccgccc acatcccgtt cgccaggcgt tcatcccact
    34081 catccgcagg aataaacaca tgaaatgcct cttccagttt gagcgtggaa cgactcggcg
    34141 cctctgccgg aaatttcagg cgcggaatgc ccatatagaa cggcgaatta ttgttgctgt
    34201 atgagtaacc ggtatagcag cagcctggtg caataaagaa tacatgcaca accggacgct
    34261 tcggcgtttc atagttcgcc agcaccccgg catcgcgcaa ggcagcgcgt agcggcacgg
    34321 tgaatttacg gcagaatttc agcaactctt tgctttcgtt ggtatcggca acttcaacac
    34381 gcagttcacc gcccttctct actacgccct gcaacatgtc gacaatgggg gtaatacgat
    34441 cttctggcgg caaatgctgc aagagttccc ccaccacaaa ccactggcgg gcaaaaatta
    34501 atgaactgaa cggcagctca cggattaact tatcgccatc atcaggttga taacattcat
    34561 aaatgacata acccgcattc tctttcacgc gggcaaaacc gaaaatttcc cgctggccgg
    34621 ctttatcggt aatttctgcg gcgcactctt tttcaaagcc cggacggcac agcaatacaa
    34681 ccttattcat gacttacgcc cttgcacttt aaacggatag caccaactaa cattaacgcc
    34741 cagcccgcga ggaagctcac gccgccaacc ggtgtgacaa atgcccacaa acgcagatgg
    34801 gacagcgcca ggcaataaag gctgccgctg aacaacaccg tgcctaacgc gaggaaaacg
    34861 ctactccagt aaaaccagat actgatgcga cgctgcattg ccaccgccag acctaagatc
    34921 gccagcgtat gaaacgcctg gtattcgagg ccggtctgga tccagcccat ctcaacggcc
    34981 cccatggttt tacttaacac atgcgcgcca aaagcgccca gagccacaaa aatgaagccg
    35041 ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg tcgtacccta cgtaaattat
    35101 tgttcataac gaaagcggaa tttttcttgt tcagcagcgg ctttcgccag gatccattgg
    35161 cgaaaggcgg ctattttacc cagttctgcc tggctgtcat gacaaaccag ataaaaagcg
    35221 tttttactga ccagaacatc attaaacggg caaacaagac gtccggcctc gatttcagat
    35281 tgcgccatca cgttatttgc cagcgccact ccctgcccgt gaatagccgc ttgcagcacc
    35341 atggcgctat gactaaaaat tggcccttgc tgaacgttga tatgatttaa ccccaactgt
    35401 cgggtatatg tctgccagtc acggcgtgac gcatcatgta ataacgtatg tttagccaga
    35461 tcttccgggg tcttcaaggg tttttcgcct gtcagcagta gcggcgaaca caccggcaat
    35521 aaatattcgg cgtacagttt ttccacccgt agccccggcc agttgccccg accataaaat
    35581 atcgccacat caacatcatc cgccagctta tcttcctgac gatcaaccgc ctggattcga
    35641 acgtcaattc ccggataagc tgaattaaag ctggaaagtc gcggaaccaa ccaatgaatg
    35701 gcgaaactgg ggagtaaact gaccgtcaac gcccccttgg cgctacgggc ctggagttta
    35761 cgcgtcgctt cggttaattg cgaaaatatc tctttgatat cgaggaaata gctttgccct
    35821 tcctcggtca gcaggagtga acgattacgg cggcggaaca gttttagccc caaaaaatcc
    35881 tcaagagact tgatttgatg acttactgcg gcctgggtca cgaaaagctc ttctgctgcg
    35941 cgagtgaaac ttaaatggcg tgctgcggca tcaaaaactc gtaaggcatt tagcggtggt
    36001 aatcgtttag acatggcttt taaactttga tgttaaatta atttaacaat tagatcacac
    36061 tgtgtaacct attagttttt ttaatctgag ccattataaa ttgtccgttg agcttctacc
    36121 agcaaatacc tatagtggcg gcacttcctg agccggaacg aaaagtttta tcggaatgcg
    36181 tgttctggtg aacttttggc ttacggttgt gatgttgtgt tgttgtgttt gcaattggtc
    36241 tgcgattcag accatggtag caaagctacc ttttttcact tcctgtacat ttaccctgtc
    36301 tgtccatagt gattaatgta gcaccgccta attgcggtgc ttttttttac cttgcgatcg
    36361 cgaattactg atccagttcg accatctctt tcacgtcagt acggttgatc tgctgtttgt
    36421 tgccattagc gtctttatac gaaatcatac cggtatcgtt atcagtctgt ggtttgccgt
    36481 cagagacgat ggtacgtccg tcattggtgt gcatcacata gttcgaaccg gaacaggcgc
    36541 tcagggcaaa agtcagcata caggcagaaa taattgcggc agtctttttc atcatcttct
    36601 ccttaaagcg attaataaat cttaatagta ttgattaaca ggctaaaatt aacgcctaac
    36661 actattcagc atatgttact tacgcggctt cgccaggata tccagataat tctgatggtt
    36721 agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc ggtgcatcaa gccgaggagt
    36781 accatgaacg tttttaatcc cgcgcagttt cgcgcccagt ttcccgcact acaggatgcg
    36841 ggcgtctatc tcgatagcgc cgcgaccgcg cttaaacctg aagccgtggt tgaagccact
    36901 cgacagtttt acagcctgag cgccggaaac gtccatcgca gccagtttgc cgaagcccaa
    36961 cgcctgaccg cgcgttatga agctgcacga gagaaagtgg cgcaattact gaatgcaccg
    37021 gatgataaaa ctatcgtctg gacgcgcggc accactgaat ccatcaacat ggtggcacaa
    37081 tgctatgcgc gtccgcgtct gcaaccgggc gatgagatta ttgtcagcgt ggcagaacac
    37141 cacgctaacc tcgtcccctg gctgatggtc gcccaacaaa ctggagccaa agtggtgaaa
    37201 ttgccgctta atgcgcagcg actgccggat gtcgatttgt tgccagaact gattactccc
    37261 cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtta ctggcggttg cccggatctg
    37321 gcgcgagcga ttacctttgc tcattcagcc gggatggtgg tgatggttga tggtgctcag
    37381 ggggcggtgc atttccccgc agatgttcag caactagata tcgatttcta tgctttctct
    37441 ggtcacaaac tgtatggccc gacgggtatc ggcgtgctgt atggcaaatc agaactgctg
    37501 gaagcgatgt cgccctggct gggtggcggc aaaatgattc acgaagtgag ttttgacggc
    37561 ttcacgactc aatctgcgcc gtggaaactg gaagcaggaa cgccaaatgt ggctggtgtc
    37621 ataggattaa gcgcggcgct ggaatggctg gcagattacg atatcaacca ggcagaaagt
    37681 tggagtcgca gcctggcgac gctggcggaa gatgcgctgg caaaacgtcc tggcttccgc
    37741 tcattccgct gccaggattc cagcctgctg gcctttgatt ttgctggcgt tcatcacagc
    37801 gatatggtga cgctgctggc ggagtacggt attgccctgc gggccgggca acattgcgct
    37861 cagccgctac tggcagaatt aggcgtaacc ggcacactgc gcgcctcttt tgcgccatat
    37921 aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg accgcgcgct ggaattattg
    37981 gtggattaat gacgaacccg caattcgccg gacatccgtt cggcacaacc gtaaccgcag
    38041 aaacgttacg caataccttc gcaccgttgt cgcaatggga agataaatat cgccagttga
    38101 tcatgctggg gaaacagctt ccggctttgc cagacgagtt aaaagcgcag gctaaagaga
    38161 ttgccggatg cgaaaaccgc gtctggctgg gatataccgt gactgaaaac ggtaaaatgc
    38221 acttctttgg cgatagcgaa gggcgcattg tgcgcggcct gctggcggtg ctgttgactg
    38281 ccgttgaggg gaaaaccgcc gccgaattgc aggcacagtc gccgctggca ttgtttgatg
    38341 agctgggatt gcgtgcgcaa cttagcgcct cacgcagcca ggggttaaat gcgttaagcg
    38401 aggcgattat cgctgcgacg aagcaggttt aagcatagaa ccgggtcgga taagacgttc
    38461 gcgtctcatc cgacctgatt gtttaaccct gacgcgccgc tttcgccatc atcttcttca
    38521 gcgcatgaga aaccgcaaca aacccaaagg tggcggtcac catcgttgcc gcgccaaatc
    38581 cagatgcaca atccatccgc ttcggccctt ctgccgtggc tttcatcgca cacacagtac
    38641 cgtctgactg cgggtacacc agcgcttcag tagaaaacac gcaatcgacg ccgagcttac
    38701 ctttactgtt tttcactacg ccaaaatcgc ttttcaggcg ctcgcgcaac ttcgccgcca
    38761 gcggatcctg aatcgttttc gccagatcgg taacctgaat ctgcgtcggg tcaatctgcc
    38821 cacccgcgcc accggtcgtg accagcggga ttttattgcg ccgacaataa gcaatcagcg
    38881 ccgctttggg ccgtacacta tcaatggcat caatcacgta cgaataaccc acgctcatat
    38941 actgcgctac gttatccggc gtcacgaaat catccaccac cgttacacgg cactccgggt
    39001 taatctggcg aatacgctcc gccatgactt ccgcttttgc cagcccgacg ttatcgcgca
    39061 gagcgtgaat ttgccgattg gtattggtga cgcacacatc atccatatcg ataagcgtga
    39121 ttgcgccaat ccccgtgcgc gccagcgctt ccgccgccca ggaaccgaca ccaccgatac
    39181 caaccacaca aatatgcgcg tcagcaaaca gttgcaacgc tttttcacca tacagacgcg
    39241 ctgtgccacc aaaacgctga cgccatgcat cactaattac cacagacata gcacctcaga
    39301 attaaaaagg gtgaggttat ccctcaccct gtcgtatccg taaaaacggt atacagaata
    39361 acacatcagc cgctaaagac gttacctgcg cccggggcgg ttttcagcac ccagacacgt
    39421 ccatagtggt tgtaccaacc tgcgcggtgt ccggcttccg gcccgatccc ttgatagata
    39481 tcgaagtgtt ggcctttgat tgcaccaccg acatccagcg ccaccatcag acgcagttcg
    39541 tactgaccat taaatttgcc gttattatcc agcaacggca cttctgccag caaggtagta
    39601 cctggcggaa taatggaacg atcagaggca actgacgcgc gaccaaccag cggcaccgca
    39661 cttgcccctt tcaccggtgc aaaagattgc ggtttaaaga agacgaaaga cgggttctgt
    39721 tccagcagct cgcgaacctc ggcttcactg tgtgtttcgc cccagtgacg aatcgcctgc
    39781 atcgacatat cttctttttt cacttcgcca cggtcgatca gcaccttacc aatgctgcga
    39841 taggcatgac cgtttttccc tgcatagctg aaaaagttaa gcggactgcc atcaccaaag
    39901 tcgatatacc cactaccctg cacatccata atgaagttat ccatcaggga gttactgtaa
    39961 gcgagaatat atttatcact caatgccccc gcgtagatct ccgcacgaga cggcagacga
    40021 ccacgttttg gcggcatacg gtaaatagga tactggaact cgccctggcg ggtatggcgc
    40081 gcctgaatta ccggcgtgta atagcctgta aactgcacgt taccatagtt gtcggcacct
    40141 tccatctgcc aggcatcaat gccgaactgg cgcatattgc gggtatcacc gcctgcgcgc
    40201 agccactctt gcaccgcgtt ataaacatta ctctggttgc catacagacg cggtgacgaa
    40261 ttacggatat ggttaatttg ctcggcaaaa tcaccggcgt taatcggcgc gccaacggca
    40321 tctggctggt tcaccagaga gaaaggctgg gtaaatttcc cgtctttata ttgctgtccg
    40381 cgatcggttg gtttggaaga gcaggcggca agcattgcca caaccgtgcc cataagaagg
    40441 tactttaccc aacgtccttt cattgttctc ttcttcaggt taaaaaataa ggcgcaacga
    40501 agataacaaa ccgccgcgtt taatgaaatg agcagcctct ccctgacgca aattttgcac
    40561 aaaaaatagg ctttagtgat ttgtttttgt tcaaaatcat gccagatccg tgatcggggt
    40621 aaaaaaaagg ttgcatgaaa acgcgagcgg agtatagtgc gcatccacgg aggcggggtg
    40681 gagcagcctg gtagctcgtc gggctcataa cccgaaggtc gtcggttcaa atccggcccc
    40741 cgcaaccaat tattgaacac cctaacgggt gtttttttgt ttctactctc ccataaaaaa
    40801 agcgccattc agcgcctttt tatcatcccc ttctcgccag cgttgcccca tcggcaaaat
    40861 acgctttaat ccccgcaaga atagactccg caacttcctg ctggaaagtc gccgttttca
    40921 gtttacgctc ttcctcaacg ttactgataa acgccgtttc gaccagaatg gagggaatat
    40981 ctggtgcctt tagtacggca aacccggcct gttcaacttg atttttatgc agcttgttga
    41041 ttttaccgag cttattcagc actgctttac caaacttaag gctgtcagca atggtcagcg
    41101 actgcaccat atcgaacatg gtgtggtcga catagcggtc accgcttttg ctcacgccac
    41161 caatcaagtc cgaggcgttc tgggtttgtg ccagatattt tgccgcagta ctggttgcac
    41221 ctttggttga gagcgcaaac accgaggaac cgctcggctg gcgactggta aaggcgtcgg
    41281 catggataga gacaaacagg tcagcacgct gtttctgggc ttttgctacg cgcacttgca
    41341 acggaatgaa gatgtcttca ttgcgcgtca tgtacacctt catattgccc tctttctcga
    41401 tcagagagcg caaacggcga gctatttgca ataccacatc tttttcgcgc gttttgtatt
    41461 tccccaccgc accggagtct tcgccaccgt ggccagggtc aagcataatg acaatcggac
    41521 gatcgcgccc tgctttacct ggttgtggac cactttgcgc tggcggcacc tgcttttcga
    41581 ggtcgccttt gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat
    41641 tggccggata gaggtccatc accagacgct ctttaaaccc ggcgaccggc gcaagggcaa
    41701 acagctgcgg ttttacgttt tgctttaatt caaaaaccat ccgtacggtt tgcgggtcaa
    41761 attgcccgac gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc
    41821 ccttgagtac cgagttcagg tttacatctt cgatatccac caccacgcgt tcagggttac
    41881 tcaacgcgaa ctgcttatat ttcagctgac gatttgattc taccgtcacg cgggtgtagc
    41941 tggacgcagg ccagacgcgc accgccacga cctggctaac cgcagccagg ctgacctgac
    42001 ttacgctcaa tagccacata gcgcccgcgc cttgcagtaa acgacgacgg ctgattgcag
    42061 tgttggatcc tgacatgcct ctcccgagca aaagaaatct aagctgtgta acaagtaaac
    42121 gactaatttg accggaaact ttagcgaaag acgcataatc tgtcatctaa taaacggtaa
    42181 acattctttt tatattcacg gcattactga taaaaaagtc gctctcgcat aaaatttaca
    42241 cttgcaccct gcgaaaaaac agaataaaaa tacaataatt tcgaataatc atgcaaaggg
    42301 gtgtgccgtg gtaaaggaac gtaaaaccga gctggtcgag ggattccgcc attcggttcc
    42361 ctatatcaat acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga
    42421 gcatgagaat ttctccagta tcgttaatga tatcgggttg ttgcacagcc tcggcatccg
    42481 tctggtggtg gtctatggcg cacgtccgca gatcgacgca aatctggctg cgcatcacca
    42541 cgaaccgctg tatcacaaga atatacgtgt gaccgacgcc aaaacactgg aactggtgaa
    42601 gcaggctgcg ggaacattgc aactggatat tactgctcgc ctgtcgatga gtctcaataa
    42661 cacgccgctg cagggcgcgc atatcaacgt cgtcagtggc aattttatta ttgcccagcc
    42721 gctgggcgtc gatgacggcg tggattactg ccatagcggg cgtatccggc ggattgatga
    42781 agacgcgatc cgtcgtcaac tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt
    42841 ttcagtcact ggcgagagct ttaatctgac ctcggaagag attgccactc aactggccat
    42901 caaactgaaa gctgagaaga tgattggttt ttgctcttca cagggcgtca ctaatgacga
    42961 cggtgatatt gtctcagaac ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga
    43021 agagaaaggc gattacaact ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg
    43081 ccgcagcggc gtgcgtcgct gtcatttaat cagttatcag gaagatggcg cgctgttgca
    43141 agagttgttc tcacgcgacg gtatcggtac gcagattgtg atggaaagcg ccgagcagat
    43201 tcgtcgcgca acaatcaacg atattggcgg tattctggag ttgattcgcc cgctggagca
    43261 gcaaggtatt ctggtgcgcc gttctcgcga gcagctggag atggaaatcg acaaattcac
    43321 cattattcag ctcgataaca cgactattgc ctgcgccgcg ctctatccgt tcccggaaga
    43381 gaagattggg gaaatggcct gtgtggcagt tcacccggat taccgcagtt catcacgggg
    43441 cgaggttctg ctggaacgca ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt
    43501 tgtgctgacc acgcgcagta ttcactggtt ccaggaacgt ggatttaccc cggtggatat
    43561 tgatttactg cccgagagca aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt
    43621 gatggcggat ttagggtaag cgatgaaaat cgccggatgc ggcaggcgta acactcgtgc
    43681 gtcgcatccg gcaattacgt ttatccccgc gaactaaaca acgccgccag accactgcgc
    43741 cgctcagtac gagtggcgat tgccgcactt aatatacgct catcggcata cagcgacaga
    43801 cggcgacgcg cgcgggtcac cgcggtgtat accagctctc gcgttactat cggcgtgcgt
    43861 tggctcggta aaatcaacgc cgcatggtcg aactccgatc cctgcgattt atgtaccgtc
    43921 atcgcccacg tagtttcgtg ctctggcaag cgactcggtt gcacagactt aatattgccg
    43981 tccggcatcg caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg
    44041 ccattaaaca acccaagcgc actgtcatta cgggcaatca tcaccggtcg accttcgtac
    44101 caacgagagt gcgaatgacg atgaattttg cgcttctgtt gcataaactg ctcaattcgc
    44161 tcattcagtc cagccacgcc aaatggccct tcccgcaggg cgcacaaaag ttggtactca
    44221 ttgaacgcct gaatgattaa atccggctcg gcacgcgctt gcagcagatc cagataacgt
    44281 ccataacccg caagagcttc ctcaagcatc gcaatataat cttcgccgct ctgtaaaagc
    44341 cgtttttcaa tatcagtaaa atcctgctga aaaacggttt tcactgccgt tttatcacca
    44401 cggttgatcg ccgcagctaa ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt
    44461 tgcagcaggc agagactgtc gcgcaaagat gccgcttctg tgccggttcc tgccggaacg
    44521 tgagttcccg tcaggcggct tagctgcccg gcacgctcgg cggtaaagcc ggcattggca
    44581 taagcgcaga tatcgcccag cacagcccca gcctcaaccg aggccagttg atcacgatcg
    44641 ccgagaaaga tcactcgcgc atgatcgggc aaggcgtcga tcagtctcga catcataggc
    44701 agatcgatca ttgacgcttc atctaccacc agcacatcaa gatgcagcgg gttaccggca
    44761 tgatgacgta aacgctggct acccggctgc gcgcccagca atcggtgcaa agtgctggca
    44821 tcttccggaa tgcgtttctt ttgttcatcg gtcagcggta actgtcgcaa agccttgccg
    44881 agagattcgg ttaagcgcgc ggcagcttta cccgttggtg cagccagacg gatacggcag
    44941 cgttcgccgt cggccatttg aattaacgct gccagcaact tcgccacggt ggtcgtttta
    45001 ccggtgcctg ggccgccgga aatcaccgag atccgccgcg tcagcgccac tgctgccgcc
    45061 actttttgcc agttaatttc atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt
    45121 agagcttcat caacctcaat ggcatgattc acctcattga agaagcgcgc taccgtccgc
    45181 tcgttacacc acatgcgatt caaataaaga cgatcgccac agaggatcat cggcgtgggt
    45241 tcatctcccc tgctgaccgc ttgagaagcc agcaagcatt cttcccaatt ttgtagctca
    45301 ccgatttcac tgacacaggt cgccaacagc ggatgcgacg cctcgttatt ttccagtcgt
    45361 gaaagcggca aacagacgtg tccctctccg gcatcatgac ttaacagtgc cgccgcgagg
    45421 gtgacggcag gatgttcatc tcccgccacg gtcagggcaa attgcacatc cagcgggcgt
    45481 agctgtttgt gctccacagc ttccagtaat tgcttttgca atttcattac gcctcctcca
    45541 gggtcatacc ggcaaacatc tcatccatca gggcaatcaa cccggcgttg ggtcgggttg
    45601 tgtaaatccc ttgttgcgga tgttctttat caacgccacg caggaacaga tagataacgc
    45661 cgccaaagtg acgatcatag tcgtaatcag caatgcgatg gcgcagataa cgatgcagcg
    45721 ccagggtata aagctgatat tgcagatcat agcggtgtgc ctgcatcgct gctgccatag
    45781 cctgttgggt gtaagccgaa ctgtcttcac ccagccagtt ggatttatag tcgagcaggt
    45841 aataacgccc ttcgtggcgg aataccaggt cgataaagcc ttttaacatg ccgcgtacct
    45901 gcatgaactc cagcggcggg cagccagcgg atagcgggtc aaactggcga attagtgcat
    45961 caagctggct ggcgatgagc ggttcgctaa tcggcaggta aaactccatc tccacctgtt
    46021 tatcgcggtc ggaaagctga ctcaggctaa cacccgtttc attgagaggt gcctggagga
    46081 cagccgtgat ccactcggtc aacaccggtt cccactgcga ttcaaagccg ccgagttcca
    46141 gtttttcctg tacccagttc gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac
    46201 tgtgcaagaa tgtccccggt gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt
    46261 cttcaacgac gctgaccacg cctgcggcat caacatccag ccgtggcatc agatcctgag
    46321 cgataccgtg accacgctgc tgcaagccgg agtagctggt gacacgccag ttatcgccgg
    46381 gcaatcgttg tagtgtcctc gcattcagtt ctgcagttaa agcatcatta acctgccagg
    46441 gctggttatc accagtttgt gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa
    46501 tacaggtgcg aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg
    46561 cactttggtg gacgtcggtg tcaccttttt tatcgccacg acggcgcacc agcggtgcaa
    46621 cgccgagact gcaatgccaa accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt
    46681 ccgccagacg ttcgacctcc gcgaggtcga cgctttctgg cgcagcatta agatccaaaa
    46741 ctgcctcaaa cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa
    46801 taaatggcaa ccagaccagc ggatattcca gccctttcga tttatggatc gtgacaatct
    46861 gcaccagatg tttatcactt tcgaggcgca tttgttggct ggaggcatta ctgtctggct
    46921 cgaggatatg ttgcgataac cagcgtacca gcgcatgttc actttccagc tgcgttccgg
    46981 cttcttgaag cagttcgctg atatgcaaga tatcggtaag acgccgctca ccgcctgccg
    47041 ttgccagcaa gttttctgca atgttacgcg ccgacatcag cgcccgcagc atcggcataa
    47101 cgccacgttt gcgccagatt tgccgataac catcgaactc ttcgactacc gcatcccacg
    47161 catgttcgtc attattcagc gtttcaatat ccagcgcatt cagccccatc attgacgttg
    47221 ccagcgcact gcgcagggtg ttctcacgtt cgggcgtcat caccgcctgc aacagccaaa
    47281 gcatttcctg cgcttccaga gtttcaaaaa cactatcgcg gttcgaaagg taaacggaag
    47341 ggatttccag caacgttaag gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca
    47401 gcacgctgat gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt
    47461 cgccccgctg tccggcttgt agccagtcgc ggatttgcgc agcacatacc tgcgccatgg
    47521 tactttgata gtcgccaacg ccgcagcttt cgccttccat cagccacatt ttcatcgcag
    47581 gctgcgtttc acctttaaag acaaaacgta acgcctgatt tttcccggct gatttcactg
    47641 gaataaacgg tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa agcttgttca
    47701 cactgttcac cattccaggt gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa
    47761 cttcactacg cgccttcata taagtgaaga tatccgcacc ccggaatgca tatatggcct
    47821 gcttcgggtc gccaatcagc aacaatgcgg tttctggctg atggtgccag atacggcgaa
    47881 aaattcggta ctgctggggg tcggtatcct gaaattcatc gatcattgcc accgggaatc
    47941 gcgtacggat cgccgctgcc aacacctcgc cgctttcgct acgcagcgcg gaatcgagcc
    48001 gacttaacat gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc gctactgttt
    48061 cgcggatctc agccaatgcg cgggtgatca ccagatcacg gatcgacaat ggttctgcaa
    48121 gcagttgatc gatcgcctca aacagtggat gtcgcggggt ttcccccccg gctttcgtgc
    48181 gatcttctaa gaaacgctgg gagaattttt ccagcgactc cggcaactga taactgtttg
    48241 tctcttcttc tgcccaggcg ctgatcttgt cgatccattt agcctgattg ctacggttaa
    48301 acttgcgtcg atcaatacca gaagattcga tcagcgcatc cagttcaccc actgcgtcgc
    48361 gccactgctg ttttaccgta tcaatacgcg ccacaatttg cgcgtgacgg gaagctagcg
    48421 tttcatcatc gggcggcggt gctttgataa ccggcgcttc gccttgcaga taacgattaa
    48481 tatcgcgcag caacgcctgc ggccctttcc aggtttcaaa gacgacctgg gctatttcac
    48541 gcggcagcgg gtagcagtgg cgacgccaga aatcggcgca ggcctggtag cgtagcagag
    48601 actcatcttc aatcagctgc tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga
    48661 gcatgcgctg gcaaaagccg tgaatagtaa agactgccgc ttcatccatc tgccgttcgg
    48721 ctaacaacaa ccactgcgcg gcttgcgctt tatcgtcgat ctcttccagc aggtgttcgt
    48781 acaatggatt gtcggtggtt tcacgcagac aggcgatgcg caactcgtgg atattgctac
    48841 ggatacgacc gcgcaattct gccgtggcag cctcggtaaa ggtgaccacc agcagttctt
    48901 caacggtcag cgggcgggga aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat
    48961 aaagcgccgc aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc aggcgctcgc
    49021 cctgtaaggg caagcgcaaa ggatctagtg tctcggcgac atcactcatt cttttcactc
    49081 atcaggggca ttgtttgctg caacgcgctg acgttctccc acactttcca gccttcagga
    49141 tgcacatatt cggctttccc gttttggctg ccggaaatct gcgacaggat agccatgcct
    49201 tgcggctcga ctaccgcctg atggaagaaa tcggccagtt tttgcggcgt cagcagttta
    49261 atctgggcca cgattttatc acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta
    49321 cttaacttcg atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc
    49381 gcctgctgga tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc ctctgcggtt
    49441 gggaaaaacg ccttgtaacg ctcccacaag aatgaaggct gtttatcatt gctttgcaaa
    49501 aggaagccca tgccccactg acgccccacg ctcattggaa acgcaaacac ggcatagccc
    49561 aattgttctt cggtacgcaa ctgattgtag aaccacggct gtacgatctg ccccaacaga
    49621 gagctatagg ctgagctggt gtattcatcg tagccagtcg gtacaaatac cgctgccagt
    49681 gcggagtcgg tgctgttacc ggctttttca aagatgacgg attgtttttt atcgaccact
    49741 acatctttgt ttcgacacca ctctgaacca tcagcgccca actgtttttg cacatcgcgt
    49801 gccagcgttg ttgcctgggc ctcggtcatg ttgccgataa ccataaactc tggtcgagcc
    49861 cctgatttta aggcgtcgcg ataagccagc acctctttca acgtaatgga gggcaaaatt
    49921 ttacgccgtt catctcgcga gaagtacggc acttgcgaga gcatctgcgc gggcataatc
    49981 gcctgctcaa acgctttacc cttttctgcg gaatccatca tctggttata ccaggacttc
    50041 gcctgctcaa gctgatcttc cgtagcggta tagctaaagt acccctcgag caatgcctgg
    50101 aacagctgcg gcagacgctg ggggtaacca ttagcattaa ccataaggcc gttgttagcg
    50161 ttggtggaaa aacttatgcc accaaccgac gcctggttgc ttaactgatc aagcgccagc
    50221 cctgcgagat aatcattgag cgcaaacatc acctgattgc gggcgctgtc catggctttc
    50281 ggattacgca aaatcaggct gacatcagct ttgggttcgc tggaaaaata acggcttggc
    50341 gcatacacca cgcgcagatt cgactcatca acaatcagct ctggatggtc atatttcttc
    50401 tctgacttaa tcagcgagaa atcgtcagga atataggggt taagctccgg taaggagagc
    50461 gcaatattgg cagctttttg ctgccagtca gcgaaagttt gttcgctgat tttatcgacc
    50521 tgatacggcg catcgacaaa ataagccgtt ttattgtgcg gctctttcgg gctgatatac
    50581 cagatacgcg cattctgcgg cgtcatcatc gccagacgtt cttttactgc tttagcatcg
    50641 taccgatcgg caatattgac tgcatccagc gtatgctcaa ccggaacgcg aatcatggta
    50701 tctgccagcc attcgacgta atccatatca cgggtgatcg acggataacg gaagtcgata
    50761 tccagcacat tcgccagttc atcgaagtat tgtttatcaa tgcctttttc acgtaacaga
    50821 ttgagatagc taaaaattgc cgccacaacc tgatcgcgat tagccaggcc tttatcggtt
    50881 aaagacgcag agatcgctaa tacgccgctg ttgccgttga cgataggatc ggagttggcg
    50941 ctaatgccct caactaatcc ctgcttttgc agccagtcgg aaagtgtacc tgggctgcga
    51001 ttaccaatca gataggtaat caattcatcc gttttactac ggaacttcgc tgagttgtta
    51061 tcgatgcgaa actcaacgcg cagaacttta cgcggcattg ccgggacgta atgaatgata
    51121 atgccctttt gcgcgtcggt gactaccggc acggtgattt ccggtttttt gctctctttg
    51181 ttcggcacgc gaccaaaggt gtccgccgcc atttttgcca actccggcag cggtttattg
    51241 ctgtaaataa ccgccttcat cagattggcg gaatagtact tctcgtggaa atctttcagc
    51301 gcctgctgca ccggattacc aggtttatcg cttaaagttt cgaggttacc accagaaaac
    51361 tttgaaccgg ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat gcgcatcccg
    51421 tcacgcgtac gcgccatggt taattcagcg ttcaccgcat tacgctcacg ttcggcatat
    51481 ttcttgtcga gcaaaggttc agcaatagca tcggccaggc ggtctaccgc accaggcaag
    51541 gcgtcgttct caacttccag atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga
    51601 ctaccgccgt gcattttgag atattcggcc agactgtcag cctgcgggta ctttttcgac
    51661 cccatcagac tcatatgttc aaggtaatgt gccagcccct ggtacgcctc gggatcttcc
    51721 agcgacccaa cgggcaccac cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc
    51781 agcaagacca ccataccgtt atccagacgt atagcctgat actggcggtt atctttatca
    51841 cttttacgga tggtttcctg aatcggctgc catcccgttt ctgcctgact taagggtgcc
    51901 caaagggcaa ctaacaacaa taatgctttg aaccaggtgc tgcggggcat tctcggacct
    51961 cataatcaac ttaattttct gtccagattc aacacgttaa cgcaaatcat ctgccagaat
    52021 ttaatcttgt gctgcacgag tcagcctatg tttatataac catcagtccg tgactggtgc
    52081 gcatcataaa gtaagcggat agattgcgca atttttatac agcactcatg actgattaaa
    52141 gcgaaacagc ggtaacagga aacgttgcga ctgttcaacg atggcctcca ttgtctctgg
    52201 tgttaattgc cgccagagcc tttgatacca gatatcatca ccttcgccac gcaccatcat
    52261 gttgccttcg taagcctgaa ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc
    52321 cagcatggca tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc cactttcagg
    52381 taacaccagc aatggcgcgg acatcccttc acgatacccc tcaatcagtt gtgagaggta
    52441 atgcaaagcc tgttcggctg caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa
    52501 aaggcgactt tcaccattac caccgctggc acagtagaca aggtgttcca gccaaagttg
    52561 cattccctgc gccacactta ataaagaggg acgccagcgc aacaggccat ccggctgcac
    52621 ctgcggcaac cagccagtta tctgcacacc gttgcaggcg agatcaattt ccatactctg
    52681 ccccggctgg cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct ggcactgagt
    52741 ttcccagaaa atttcaccaa aagcgccata cggtaaatcc cctgccgccc ggaagcgacg
    52801 gaacaagcgt tcggcatcat cctgctcaac cagtgcattc aataactgct gattgatttg
    52861 ataacggcta agcccttcca gaataaatgg ctcggtgtcg gggatttcgc tgtcttcagt
    52921 acggaagttc acctgcaaac gcatctggaa gaacgcccgc accggatgtg cccagaatcg
    52981 ttgtagcgtt tccagcggca cggtttccgg taaggtaaac ggcagcggct gaacaaattc
    53041 agaatgtgct ttaccagcct ggctggccgc aggtagccat tcacgagcat agctttgtcg
    53101 ttcgcctggc tgatagtttt gtggatcaaa cggcattcgg gtatggaggc aagtaagatg
    53161 cgcttttacc cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg gtagataatg
    53221 actttgcccg atgtagtcga tcagttcctg caccagtacc gacgggaaac gttcactgtt
    53281 atcctgaatg gaacgcccga tatagctgat atagagtttt tgctgcgcgg aaattaacgc
    53341 ttccaggaac agatagcggt cgtcatcgcg acggctacgg tcgccacgct tcggtttctg
    53401 gctcatcagg tcaaagccca atggcgcaag ctgacgtgga taaacgccgt cgttcattcc
    53461 cagcaggcaa accactttga acggaattga acgcattggc atcagagtac aaatgttaac
    53521 cggtccggcg agaaaacgct ggctgatacg ttcttgatcc agacgctgtg ccagttcatc
    53581 acgcaatagt gacagcggca ccgcgtcgcc atactgcgca cctaaacctt cggcgataat
    53641 cgcctgccat tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa
    53701 gaaggcgctg agcatatcgc gacaaaccgg caaccactct tctagcggac gctcctgtgc
    53761 cagcccgcga cgccagatgt ttagctgcat tagcagtgaa gccagatgcc ccaccagttc
    53821 tgcaattaag ccgctcgatt catcataagg tagaaccgat tgccactcgc cctgcgcgct
    53881 ctccatcgcg tagcccagca acatacgcgt caggccaaat cgccaggtgt gttgtccggt
    53941 ggcggggagt tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa tgccggattc
    54001 gttgacccac tggcgtaaat aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag
    54061 caccggcaca tccagtaatg ccagcacgtc ttccgacaca aagcggctgt ctggcaacga
    54121 taacaggctg ataaacgctt ccagtaccgg atgcgactgc cgcgcccgac ggtcggaaat
    54181 ggcgtaaggc aggtaacgat ccgcaggtgc gctaccaaac acagcccgaa taaacggact
    54241 gtagctgtcg atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc
    54301 ttccagcatc gccagcaggc gatcgtgcaa aacttcaact tcacgctgcg ggctatggca
    54361 aacgtggaag gtgatactgc tatccagtgg atcaagcggg cgtttgttat cgctacggga
    54421 aaactcttcg atgttcacac cagcaacggc acggttttcc agttccagaa tgtcagactg
    54481 aatgttatgc agcaggttat ctggcgtgac atcgacaaag gcgtccagct cctggctgct
    54541 ctccaggtca gaaaggagat aaatgtagtc gcgcccaagc ttaccccatg aagccagcag
    54601 cgggttgccg acatcctgtt caccatcgct gttaaagagc tgcccggcat tttcgctatc
    54661 acgaaatagc ggcaattcgc gatcttcaaa actgtgtcga cgctgacggg tcagcagttt
    54721 cgccagataa gcaggatctt taatatcgcc ccagtaataa cggcaggggt tggtaaacag
    54781 gagatggatt tcaatatgtt tacccagcgc ctgtagcgcc tggagataaa caggcggtaa
    54841 cgcggaaata ccgcatataa agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga
    54901 ctccagcgtt tcgataaagc gctgatagag attggcgcgg tgccagcgcg gttgcccgag
    54961 ttgatgggta tattccacca gcgccttcca caacggggct tgccaggcct gtgcttctcc
    55021 cagcccttca accaaatgtc ctgtttccca ctgtgccagc cagtccggac gatagaccag
    55081 atactggtca aacaggtccg ccgcttttga ggaaagctgg aacagttttc gcttgtcgct
    55141 atcgtcagtc agataatgcc gcaacagggt aaagtcttcg cgctccagca attgcggcag
    55201 cagagtcatc agtttccagc tcatgctctg tttgttaaag gcgctctctt tggggatttc
    55261 cggtaacacc cggacgaaca tatcccagat aaagctcgct ggcagcggaa aatcaatgtt
    55321 tgccgcaata ctaaactttt gcgacagggt catttgcagc cactgcgcca taccggtact
    55381 ttgcaccaga atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa
    55441 ctccatcaac gcttccagca cgtccagacg attggaatgg tagaccctta acatagcggc
    55501 tcctgactac tgacgattcg ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg
    55561 tgaaactagc gtaacgctga tgctgacaca tcccgcctgc gatgtctgca ttcggttgac
    55621 ctgccagttg gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccaac catgccgcca
    55681 gagctgttgg tactggtttc tgctggcgag actgttcatt aatgttcgct ggatacccga
    55741 taacgcagtg acaatcatca ccatcagcac catcgcaacc attacctccg gcaggctaaa
    55801 gccttgttga ttcttcaggg aagctgacat aacgccctct ctttcagcgg acaaaaatcg
    55861 ctccagccgc gtggcgaaaa aacaatgttt ccatcaatga cttcgcctgt tcgccacaac
    55921 gaaacgcctt cataaccggc aaccaataag gcttcattat ctgccagtaa acgcaaacaa
    55981 acccgggcat cggttccagc gtactgcgag cactgaactg cgggctgcgt ctgccaggag
    56041 tgcattttcc cccacgccag cgccgactga acgatggcct ggcggcgcaa tgactgactt
    56101 tctatgctca cgcgagaggc aaaactgcga tcctgctgac tcattccttg caatagcaag
    56161 ctacccaaaa ccagcagcat caggaccaga gccagtgacg aaacaccttt ttcgcggttc
    56221 acaggttgaa tcctgtcacg ctatagctgg catccaccac ggtttgcggt tcagacttac
    56281 tggcagcacg catattaacc gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga
    56341 cctgaaaagt gtcgataatg atggcatccg gattagtgac tttatcccag cccttacctt
    56401 cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc cttcagacga aatccaatct
    56461 ggtcagactc ttttaccggt tcgcgatccc agataccgtt actgttcgca tcccactgca
    56521 caatgataca gtcaccctgt ccgacaattt ccagcccttc gccggtacag ctgccatgac
    56581 aataacccgc cctctggaga tgcttcgcga cggtaaatac ccgcagccag atttcatctt
    56641 ccagcgccag cttacgggtg ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg
    56701 cagcccccag caacaatacg ctactgatcg ccatagcaat caacacttcc agcagagaaa
    56761 aaccttgctc ttttacaggc atccttctgt ttctccttgc tgacaaagcc ggagtcttcc
    56821 ccacggcgaa accaccagcc accactcgcc cgttgagttt ttgaagcgaa tatgcccggc
    56881 ccatgcggta ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc tcatttagac
    56941 ttcgggccag cgtggcacaa agaccaatgg tgaactgcca tgacaggtat tggccccagc
    57001 agcggaactc acaaggcacc ataacgtccc ctccctgata acgctgatac tgtggtcgcg
    57061 gttatgccag ttggcatctt cacgtaaata gagcaaatag tcccgcgcct ggctggcggt
    57121 ttgccatagc cgttgcgact gctgccagta ttgccagcca tagagtccac ttgcgcttag
    57181 catgaccaaa atcagcatcg cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt
    57241 catgccggca gtatggagcg aggagaaaaa aagacgaggg ccagtttcta tttcttcggc
    57301 gcatcttccg gactatttac gccgttgcag gacgttgcaa aatttcggga aggcgtctcg
    57361 aagaatttaa cggagggtaa aaaaaaccga cgcacactgg cgtcggctct ggcaggatgt
    57421 ttcgtaatta gatagccacc ggcgctttaa tgcccggatg tggatcgtag ccttcaatct
    57481 caaagtcttc gaaacggtag tcgaagatgg attcgggttt acgtttgata atcaacttcg
    57541 gcagcggacg cggttcgcgg cttaattgca gatgagtttg atccatatga ttgctgtaca
    57601 ggtgcgtatc gccaccggtc cagacaaaat cacccacttc cagatcgcac tgctgcgcca
    57661 tcatatgcac cagtaacgcg tagctggcaa tgttgaacgg caggccgagg aagacgtcac
    57721 aggagcgctg ataaagctgg caagagagtt tgccgtctgc cacatagaac tggaagaatg
    57781 catggcacgg tgccagcgcc attttatcca gttcgcctac gttccacgct gaaacaataa
    57841 tgcggcggga atccgggtcg tttttcagct ggttcagtac cgtagtgatc tggtcaatat
    57901 gacgaccatc tggcgttggc caggcgcgcc actgtttacc atacactggt ccgaggtcgc
    57961 cgttttcatc ggcccattcg tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt
    58021 tagtgtcgcc ctgcagaaac cacagcagtt cgtggatgat ggaacgcagg tggcaacgtt
    58081 tagttgtcac cagcgggaat ccatcctgca ggttaaaacg catctgatga ccaaaaatgg
    58141 aaagcgttcc ggttccggta cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca
    58201 tcagttctaa atactgtttc atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat
    58261 atgcccagac catcatgatc acacccgcga caatcatcgg gatggaaaga atttgcccca
    58321 tgctgatgta ctgcacccag gcaccggtaa actgcgcgtc gggctggcgg aaaaactcaa
    58381 caatgatgcg aaacgcgccg tagccaatca ggaacaagcc agagacagct cccattgggc
    58441 gcggtttacg aatatacagg ttgaggataa taaacagcac cacaccttcc agcagcagct
    58501 cgtaaagctg tgatgggtgg cgcggcagca caccgtaagt gtcgaaaatg gattgccact
    58561 gcgggttggt ttgcagcagc aaaatatctt ctgtacggga gcctgggaac agcatggcaa
    58621 acgggaagtt cgggtcaacg cggccccaca attcaccgtt aataaagttg cccagacgcc
    58681 cggcaccaag accaaacgga atgagtggtg cgataaaatc agaaacctgg aagaaggaac
    58741 gtttagtacg gcgggcaaag ataatcatca ccacgataac gccaatcagg ccgccgtgga
    58801 aagacatgcc gccgtcccag acacggaaca gatacagcgg atcggccata aactgcggga
    58861 aattgtagaa cagaacataa ccaatacgtc ccccgaggaa gacgccgagg aagcccgcat
    58921 agagtaagtt ttcaacttca tttttggtcc agccgctgcc cggacgattc gcccgtcgtg
    58981 ttgccagcca cattgcaaaa atgaaaccca ccagatacat caggccgtac cagtgaagcg
    59041 ccacgggtcc tattgagaaa atgaccggat caaactccgg aaaatgcaga tagctactgg
    59101 tcatctgtca ccacaagttc ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg
    59161 ccgcaggttt caggcgctcc aaaggtgcga ataatagcac aaggggacct ggctggttgc
    59221 cggataccgt taaaagatat gtatatgatc cgcgctataa ccctccgcga atcagcccgc
    59281 ccatgccgcg acgctccata aaggctgcaa cctgatggcg aacttcggtc gccagttgcg
    59341 cttccagact acgctgcgca agattttctg cttcggcata atcaatgcgc cgcagcaggt
    59401 attttacccg cgctacagaa cgtccgttca tagacagatg gcgataccca agcccaatga
    59461 ggattgccac gcacatggga tcgcccgcca tttcaccgca caaacggaga tcgattccat
    59521 gtatttccgc ttcccgggcg atcatcgcca gagctcgtaa cattgcagga tgaagactgt
    59581 cataaatgtt cgccacccgg gtattgttgc gatcaacggc cagaatgtat tgagtcagat
    59641 cgttggtgcc aacagagatg aaatcgaccc gctttgccag atgcggcagc ataaatacca
    59701 ttgacggcac ttccagcatg atgccgatac gtggtttggg aatttcgtaa ccgatcatct
    59761 cctcgacttc acgtccggca cgttcaatca ggcggcgtgc ttcatcaact tcatcgaggc
    59821 ttgtgaccat cggcaacaga atattcaggt tgcccgtagc ggcattagca cgcagcatcg
    59881 cccgcacctg gatcaagaag atctccggct gatcgagcgt aatgcgaatc ccacgccaac
    59941 ccaggcatgg attctcttcg ctgatcggca tgtaaggcag ctgcttatct gctccgacat
    60001 ccagcgtacg caaggtgacg ggtttatcat tgaacatttg cagcatcccc tgatactgcg
    60061 ccacctgttc ttcttccgac gggaaaccac tttgcagcat gaatgggatt tcagtgcgat
    60121 aaagaccgat gccatcaata cggctgccca gtttttcttc atgttccggg cttaaaccag
    60181 cattgagcat gacttttata cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat
    60241 cttccgccag acggctaagc tcaatctctt cactaattag ccgctgatat tcttgcagca
    60301 gtaccggctc cggatcgacc agcaattcac cgcgatagcc atcaacgatc agcgtcctac
    60361 gatgcagcac cgaaggctga atatccgcgc ccatcacggt agggatcccc agcgcacgta
    60421 ccatgatcgc agcatgggag ttggctgcgc catctcgcac gacaacaccg actaagcgat
    60481 cctggggcag ctcagcaaga gttgtcgctg acagttcatc tgccaccaga atgaaacgtt
    60541 ccggccaggc gttcggccct tgattagcgt catcaagatg aaacagcaat cgctgaccca
    60601 gcgcacgtaa atcgccagcc cgctctttga gatagttatc gcttagcgcg gcaaactgtt
    60661 cggcaaattt ttcaatgacc gtttttaccg cccactctgc caccgagcct ttatcaacct
    60721 cggcaaacaa ttcgcgacgc agccgggtgt ccgaaagtag gtgcgagtaa agatcgaaaa
    60781 tagccgccgt ttctttttgt gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt
    60841 ttgccgcttc ttccagcgcc ccggtcagtc gttcgcgttc cagagccgga tccagcgttg
    60901 atgcctgata cacctgttcc attaaaggta acgtggcatc ctgccagcct tcggcaatcg
    60961 ccacaccagg tgctgccggt aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg
    61021 cagtcaactg cgactgagaa agaatagctg ccatctgggt ggcaagcgtc accaggaagg
    61081 attcttcact ttcgtcatac tggcgcaact ctcgttgctg taccaccagt acaccaagca
    61141 actggcgacg ttgaataatt ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg
    61201 aggggatgta tttgaagctg gggtgctttt gcgcatctgc aaggtttatc ggttccgcca
    61261 gcctgccaac caggccgacg atcccttcat caaacgcgag cgttacagtg cgaccgcgtg
    61321 gttttttcag cccccgggtc gccatcaggt agtaacaacg tcgatcatga tcggccaggt
    61381 agaccgaaca gacctcggta tccatcgcaa gacagatgtc ggtaaccaga atatttaacg
    61441 cctcattcag gcgtggtgcg ctggctacct tttcgactat ttcgcgcagg cgagtgagca
    61501 taattggcgt gacttaacct cttttacgtc gataagcaga tgtgttttgt ggttttggcg
    61561 tattttcctg cagtgacatc accacactcg cgaactcttt cattacccta cggtagacat
    61621 cacgtttaaa tgacaccacc tgtctgaccg gataccagta acttacccat cgccagccgt
    61681 caaactctgg tgtactgctg gtttgcatat tgatttctgc atcgccgctc accagctgca
    61741 agagaaacca tttttgtttt tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac
    61801 gtttcggtaa tttgtagcgc aaccagttac gcgttgaagc aaggattcga acgtctttgc
    61861 ggcttaatcc tacttcttca aacaattcac ggtacatcgc ctgctctgcg gattctccgg
    61921 ggttgattcc gccttgcgga aattgccagg agtgctgacc aaatcgccgg gcccacatta
    61981 cctgcccctg gcgattacaa atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa
    62041 tcaccggact acctcaaaat aaagctttat atacgaatga ttgtttcata ctccaagaag
    62101 acggtaaacc actctctgca gggcattaca cactaataac aattgaataa ctcacagtta
    62161 tgtgcagagt tataaacaga ggaaggggtg gatagccgtt tttattcacc ttttctgtgg
    62221 atagagttgt gaagaactac ggaattacta cgggaaaacc cggagcattc tgaataagcc
    62281 cgatatagac attttaaaat attcattatt agtcaatgca ttacatgtaa tttgagtaat
    62341 gaaaaagagt aattcgtgac ccaggtcaca cctctcattt acgggttggc gaaagatcaa
    62401 ccaatgccgt atttatccac agaatgtgcc actaagttaa gcactgaacc actaaaaact
    62461 ggagtttcgt cgcacgtcaa ggctgtaaat ggaaacagta gtggaggttt ttcacagtta
    62521 tcccagcttt ctgtggataa catggtgtaa gatcctgttt attttcagtg accagatttg
    62581 gaaaacctat tgcagtgttg cgcaactcga ttaccggcag cctaaaaagc aatataaatc
    62641 agagaattga acaacacatg tggaaaaagt tacactgcga atattcggca cataattgct
    62701 gtttgttttt taaccaaggt atcatgacat gtcccaacct cgcccactcc tctctcctcc
    62761 cgaaactgaa gaacagttgt tagcgcaagc acagcaactt tctggttata cattgggaga
    62821 actggcggca cttgccgggc tggttacgcc agagaattta aaacgcgata agggctggat
    62881 tggcgtgtta ctggagatct ggctaggtgc cagcgcaggg agtaaacctg agcaagattt
    62941 tgctgctctg ggcgtggaac ttaaaactat ccctgtggat agtcttggtc gtccgctgga
    63001 aacaacattc gtttgtgttg ccccgttaac gggtaatagc ggggtgacct gggaaaccag
    63061 ccatgtgcgc cacaagctca aacgcgtact gtggataccg gttgaaggtg agcgcagcat
    63121 cccgctggcg cagcgtcgcg taggatcacc gttgctgtgg agcccgaatg aagaggaaga
    63181 ccgacagcta cgcgaagact gggaagaatt aatggatatg attgttctcg gtcaggttga
    63241 gcggatcacc gctcgtcacg gggagtattt acagatacga ccgaaagcag cgaatgcgaa
    63301 agcgcttacc gaagccattg gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt
    63361 ttatttgaag aagaatttca ccagtgcact actggcccgt cattttctga tccagtagcc
    63421 atcgctttga cctgccgctt tccgggcata taattaccgc ttcatttttt tggcagggct
    63481 ttttagatgt tatttgcatg gataaccgat cctaacgcct ggcttgcgct cggtacgctg
    63541 acgctgctgg agatcgttct tgggatcgac aatattattt tcctttctct ggtggtggca
    63601 aagctcccca cagcacaacg tgctcatgcg cgccgtctgg ggttggcggg agccatggtt
    63661 atgcgtctgg cgctgctggc atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt
    63721 acaatattca gtcaggaaat ttccgcccgt gatttgattc tgcttctggg tggcttgttc
    63781 cttatctgga aagccagcaa ggaaatccac gaatccattg aaggtgaaga agaagggctg
    63841 aaaacacgcg tttcatcatt ccttggcgct atcgtgcaga ttatgctgct ggatattatc
    63901 ttcagcctcg actcggtgat taccgctgtg ggtctgtcag atcacctgtt tattatgatg
    63961 gccgccgtgg tgattgccgt aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt
    64021 gtcgaacgcc atccttcggt aaaaatgctg gcgctctctt tcctgattct ggtcggcttt
    64081 accctgattc tggaaagttt cgacatccac gtaccgaaag gttacatcta cttcgcgatg
    64141 ttcttctcta ttgcggttga aagcctcaac ctgattcgca acaaaaagaa cccgctctga
    64201 tactccgtac gctctcctgc gggagggcgt actcactcct gttaccctca cagtttaaga
    64261 ttttcctgct ttcaagatat ataacgtcgg tttataaaca gactattatc ataggtgggc
    64321 caaatgagga taaccggatg aaaaaatggg cagtaataat ttctgcagtc ggactggcgt
    64381 ttgctgtttc cgggtgttcc agtgattacg tcatggcgac caaagatggc cgtatgattt
    64441 tgaccgatgg aaaacctgaa attgatgatg ataccgggct ggtgagttat cacgatcagc
    64501 aaggtaacgc gatgcaaatt aaccgtgatg atgtttcgca aattattgaa cgttaacaaa
    64561 taaggtcagc atccggctgt aatgccgatc agttaag
//
LOCUS       DAZPEP010000008        59895 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZPEP010000008 DAZPEP010000000
VERSION     DAZPEP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 59895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59895
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.008"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="ACHUT9_000493"
     CDS             complement(<1..1474)
                     /gene="ggt"
                     /locus_tag="ACHUT9_000493"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFR1347060.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGMGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
     gene            1594..2034
                     /locus_tag="ACHUT9_000494"
     CDS             1594..2034
                     /locus_tag="ACHUT9_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFR1347061.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="ACHUT9_000495"
     CDS             complement(2021..2764)
                     /gene="ugpQ"
                     /locus_tag="ACHUT9_000495"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1347062.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="ACHUT9_000496"
     CDS             complement(2761..3831)
                     /gene="ugpC"
                     /locus_tag="ACHUT9_000496"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFR1347063.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="ACHUT9_000497"
     CDS             complement(3833..4678)
                     /gene="ugpE"
                     /locus_tag="ACHUT9_000497"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFR1347064.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="ACHUT9_000498"
     CDS             complement(4675..5562)
                     /gene="ugpA"
                     /locus_tag="ACHUT9_000498"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFR1347065.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="ACHUT9_000499"
                     /pseudo
     CDS             complement(5660..6724)
                     /gene="ugpB"
                     /locus_tag="ACHUT9_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            complement(7008..7721)
                     /gene="livF"
                     /locus_tag="ACHUT9_000500"
     CDS             complement(7008..7721)
                     /gene="livF"
                     /locus_tag="ACHUT9_000500"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFR1347066.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(7723..8490)
                     /gene="livG"
                     /locus_tag="ACHUT9_000501"
     CDS             complement(7723..8490)
                     /gene="livG"
                     /locus_tag="ACHUT9_000501"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFR1347067.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(8487..9764)
                     /gene="livM"
                     /locus_tag="ACHUT9_000502"
     CDS             complement(8487..9764)
                     /gene="livM"
                     /locus_tag="ACHUT9_000502"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFR1347068.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(9761..10687)
                     /gene="livH"
                     /locus_tag="ACHUT9_000503"
     CDS             complement(9761..10687)
                     /gene="livH"
                     /locus_tag="ACHUT9_000503"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFR1347069.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(10735..11844)
                     /gene="livK"
                     /locus_tag="ACHUT9_000504"
     CDS             complement(10735..11844)
                     /gene="livK"
                     /locus_tag="ACHUT9_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFR1347070.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            12268..12651
                     /gene="panM"
                     /locus_tag="ACHUT9_000505"
     CDS             12268..12651
                     /gene="panM"
                     /locus_tag="ACHUT9_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFR1347071.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWIADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="ACHUT9_000506"
     CDS             complement(12850..13953)
                     /gene="livJ"
                     /locus_tag="ACHUT9_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFR1347072.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="ACHUT9_000507"
     CDS             complement(14224..15078)
                     /gene="rpoH"
                     /locus_tag="ACHUT9_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFR1347073.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="ACHUT9_000508"
     CDS             complement(15323..16381)
                     /gene="ftsX"
                     /locus_tag="ACHUT9_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFR1347074.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="ACHUT9_000509"
     CDS             complement(16374..17042)
                     /gene="ftsE"
                     /locus_tag="ACHUT9_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFR1347075.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="ACHUT9_000510"
     CDS             complement(17045..18541)
                     /gene="ftsY"
                     /locus_tag="ACHUT9_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFR1347076.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            18691..19287
                     /gene="rsmD"
                     /locus_tag="ACHUT9_000511"
     CDS             18691..19287
                     /gene="rsmD"
                     /locus_tag="ACHUT9_000511"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFR1347077.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            19277..19546
                     /locus_tag="ACHUT9_000512"
     CDS             19277..19546
                     /locus_tag="ACHUT9_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFR1347078.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19549..19908)
                     /locus_tag="ACHUT9_000513"
     CDS             complement(19549..19908)
                     /locus_tag="ACHUT9_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFR1347079.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            20049..20675
                     /locus_tag="ACHUT9_000514"
     CDS             20049..20675
                     /locus_tag="ACHUT9_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFR1347080.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            20749..22947
                     /gene="zntA"
                     /locus_tag="ACHUT9_000515"
     CDS             20749..22947
                     /gene="zntA"
                     /locus_tag="ACHUT9_000515"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFR1347081.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="ACHUT9_000516"
     CDS             complement(23049..23294)
                     /gene="tusA"
                     /locus_tag="ACHUT9_000516"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFR1347082.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            23515..24180
                     /locus_tag="ACHUT9_000517"
     CDS             23515..24180
                     /locus_tag="ACHUT9_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFR1347083.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            24253..24810
                     /gene="dcrB"
                     /locus_tag="ACHUT9_000518"
     CDS             24253..24810
                     /gene="dcrB"
                     /locus_tag="ACHUT9_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFR1347084.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(24814..26031)
                     /locus_tag="ACHUT9_000519"
     CDS             complement(24814..26031)
                     /locus_tag="ACHUT9_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1347085.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMLWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            26163..27212
                     /locus_tag="ACHUT9_000520"
     CDS             26163..27212
                     /locus_tag="ACHUT9_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1347086.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            27267..27854
                     /gene="acpT"
                     /locus_tag="ACHUT9_000521"
     CDS             27267..27854
                     /gene="acpT"
                     /locus_tag="ACHUT9_000521"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFR1347087.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            27965..29539
                     /gene="nikA"
                     /locus_tag="ACHUT9_000522"
     CDS             27965..29539
                     /gene="nikA"
                     /locus_tag="ACHUT9_000522"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1347088.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            29539..30483
                     /gene="nikB"
                     /locus_tag="ACHUT9_000523"
     CDS             29539..30483
                     /gene="nikB"
                     /locus_tag="ACHUT9_000523"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFR1347089.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            30480..31313
                     /gene="nikC"
                     /locus_tag="ACHUT9_000524"
     CDS             30480..31313
                     /gene="nikC"
                     /locus_tag="ACHUT9_000524"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFR1347090.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            31313..32077
                     /gene="nikD"
                     /locus_tag="ACHUT9_000525"
     CDS             31313..32077
                     /gene="nikD"
                     /locus_tag="ACHUT9_000525"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFR1347091.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            32074..32880
                     /gene="nikE"
                     /locus_tag="ACHUT9_000526"
     CDS             32074..32880
                     /gene="nikE"
                     /locus_tag="ACHUT9_000526"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFR1347092.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            32886..33287
                     /gene="nikR"
                     /locus_tag="ACHUT9_000527"
     CDS             32886..33287
                     /gene="nikR"
                     /locus_tag="ACHUT9_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFR1347093.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(33407..33766)
                     /locus_tag="ACHUT9_000528"
     CDS             complement(33407..33766)
                     /locus_tag="ACHUT9_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFR1347094.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(33763..34036)
                     /locus_tag="ACHUT9_000529"
                     /pseudo
     CDS             complement(33763..34036)
                     /locus_tag="ACHUT9_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            complement(34109..35233)
                     /locus_tag="ACHUT9_000530"
     CDS             complement(34109..35233)
                     /locus_tag="ACHUT9_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1347095.1"
                     /translation="MRHLRNIFNLGIKELHSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(35233..37968)
                     /gene="rbbA"
                     /locus_tag="ACHUT9_000531"
     CDS             complement(35233..37968)
                     /gene="rbbA"
                     /locus_tag="ACHUT9_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFR1347096.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(37965..39032)
                     /locus_tag="ACHUT9_000532"
     CDS             complement(37965..39032)
                     /locus_tag="ACHUT9_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1347097.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(39095..39208)
                     /gene="yhiY"
                     /locus_tag="ACHUT9_000533"
     CDS             complement(39095..39208)
                     /gene="yhiY"
                     /locus_tag="ACHUT9_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFR1347098.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(39398..41020)
                     /locus_tag="ACHUT9_000534"
     CDS             complement(39398..41020)
                     /locus_tag="ACHUT9_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1347099.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTINDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(41282..42889)
                     /locus_tag="ACHUT9_000535"
     CDS             complement(41282..42889)
                     /locus_tag="ACHUT9_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFR1347100.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKSDITYKVNGLS
                     IIPENFFTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNAN
                     TAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNKLLYN
                     LLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMDG
                     NKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCHYDSETKVL
                     TSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQHMDDRLIHLIRS
                     NAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTM
                     GLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            43274..44326
                     /locus_tag="ACHUT9_000536"
     CDS             43274..44326
                     /locus_tag="ACHUT9_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFR1347101.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(44642..45844)
                     /locus_tag="ACHUT9_000537"
     CDS             complement(44642..45844)
                     /locus_tag="ACHUT9_000537"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFR1347102.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            46076..47575
                     /gene="pitA"
                     /locus_tag="ACHUT9_000538"
     CDS             46076..47575
                     /gene="pitA"
                     /locus_tag="ACHUT9_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFR1347103.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(47819..48154)
                     /gene="uspB"
                     /locus_tag="ACHUT9_000539"
     CDS             complement(47819..48154)
                     /gene="uspB"
                     /locus_tag="ACHUT9_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFR1347104.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            48545..48979
                     /gene="uspA"
                     /locus_tag="ACHUT9_000540"
     CDS             48545..48979
                     /gene="uspA"
                     /locus_tag="ACHUT9_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFR1347105.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(49078..49131)
                     /locus_tag="ACHUT9_000541"
     CDS             complement(49078..49131)
                     /locus_tag="ACHUT9_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347106.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            49297..50766
                     /gene="dtpB"
                     /locus_tag="ACHUT9_000542"
     CDS             49297..50766
                     /gene="dtpB"
                     /locus_tag="ACHUT9_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFR1347107.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(50815..51567)
                     /gene="rsmJ"
                     /locus_tag="ACHUT9_000543"
     CDS             complement(50815..51567)
                     /gene="rsmJ"
                     /locus_tag="ACHUT9_000543"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFR1347108.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(51575..53617)
                     /gene="prlC"
                     /locus_tag="ACHUT9_000544"
     CDS             complement(51575..53617)
                     /gene="prlC"
                     /locus_tag="ACHUT9_000544"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFR1347109.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            53820..54662
                     /gene="rlmJ"
                     /locus_tag="ACHUT9_000545"
     CDS             53820..54662
                     /gene="rlmJ"
                     /locus_tag="ACHUT9_000545"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFR1347110.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            54734..56086
                     /gene="gorA"
                     /locus_tag="ACHUT9_000546"
     CDS             54734..56086
                     /gene="gorA"
                     /locus_tag="ACHUT9_000546"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFR1347111.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(56140..56223)
                     /gene="dinQ"
                     /locus_tag="ACHUT9_000547"
     CDS             complement(56140..56223)
                     /gene="dinQ"
                     /locus_tag="ACHUT9_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFR1347112.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            56251..56424
                     /locus_tag="ACHUT9_000548"
     CDS             56251..56424
                     /locus_tag="ACHUT9_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347113.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            56963..57316
                     /gene="arsR"
                     /locus_tag="ACHUT9_000549"
     CDS             56963..57316
                     /gene="arsR"
                     /locus_tag="ACHUT9_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFR1347114.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            57370..58659
                     /gene="arsB"
                     /locus_tag="ACHUT9_000550"
     CDS             57370..58659
                     /gene="arsB"
                     /locus_tag="ACHUT9_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFR1347115.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            58672..59097
                     /gene="arsC"
                     /locus_tag="ACHUT9_000551"
     CDS             58672..59097
                     /gene="arsC"
                     /locus_tag="ACHUT9_000551"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFR1347116.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(59231..59527)
                     /locus_tag="ACHUT9_000552"
     CDS             complement(59231..59527)
                     /locus_tag="ACHUT9_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1347117.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            59634..>59895
                     /locus_tag="ACHUT9_000553"
     CDS             59634..>59895
                     /locus_tag="ACHUT9_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1347118.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT        14010 a        16147 c        15122 g        14616 t
ORIGIN      
        1 gcacggtggt aataatccga ctcccgccag gactaccggt aaccagccaa gttttaccgt
       61 ctttcaccac aatggttggt gacatcgatg acagcggacg tttattcggc ccgacggcgt
      121 tggcatcacc gcccaccagc ccgtaaatgt tcggtacgcc cggtttggcg gagaaatcat
      181 ccatctgatt attaagcaga ataccgctct cgcccgcgac aatgcccata ccgaaggtgg
      241 tgttcagcgt ataggtcacc gccaccgcgt taccgtcttt atccactaca gagtaatggg
      301 tagtttgatt actctcataa ggcgcaagct tgccagggcg aatctcactg gacggcttcg
      361 ctttgttgat atcaatttga tcggcaatgg atttggcata ggctttattg gtcagtgcct
      421 gccacgggac tttgacaaaa tccgggtcgc caagatattc cgagcggtcg gcgtaggcgt
      481 atttctccgc ttctgccatg atttgcatcg catcggcgct gccaaagccg tatttcttca
      541 tatcgaagtt ttccagaata ttgaggattt gtacgatatg gatcccgccg gaggatggcg
      601 gtggcatgga gtaaacctga tacccgcgat aatcgccgct tatcggagtg cgttcgaccg
      661 ctttataggc cgctaaatct tctttagtga tcaagccacc gtttttctgc atctcctggg
      721 cgatctgttc cgcaatcgtg cctttataga attcgtccgg gccgttttca gcaatcatct
      781 ccaggctctt tgccaggttc gcctgcacca gcgtgtcgcc ctttttcagc ggctcgccct
      841 ctttccagaa gatagcttta ctgttttcgt gattcggcaa cacttcgcta ccgtaggttt
      901 tgagatcgtc agccagcgcg tcgttaacga taaaaccatc gcgtgccagt ttaaacgcgg
      961 gctgcacgac tttgttcagc ggcatggtgc cgtatttatc cagcgccagc gagaaacctg
     1021 ctaccgtacc cggtgtgccg gaagccagat gcgaagtgag tgattttttg ctgtccgggt
     1081 tgccctgatc atcgaggaac atatcgcggg tcgctttggc gggtgccatt tcgcggaaat
     1141 cgatagccgt ggtattgcca tttttcgagc ggattaacat aaaaccacca ccgcccagat
     1201 tccctgcctg cggatgcgtt accgccagcg cgtagcccac cgccacggcg gcatcaacgg
     1261 cattcccgcc ctccttgagg atatccaccc ccacctgagt ggcagtggcg tccacagacg
     1321 ctaccattcc ctgtttcgcg cgtaccgggt ggaagacatc ttcctccaca ccatacgaga
     1381 cgggcggcgc aggaggcgcg gcggcggcgc taaaacaact tcctgagagc agagcagcaa
     1441 tggccacccg gcgtaaaaac gtcggtttta tcatcgttat tctccagaga ttaaggggca
     1501 accccagcta agcctggtat ataactctga attaatcatc gttttgtcgg gaagcgagta
     1561 aacttaaagg atatcttcag aggaggatac gcgatgaaac gacttctgat tcttacggca
     1621 ctcctgccgt ttgtcggctt tgcacagccc attaatactc tgaacaaccc taaccagccg
     1681 gggtatcaga tacccagcca gcagcggatg caaacccaga tgcagactca gcaaatccag
     1741 caaaaaggga tgctgaatca gcaactgaaa acgcaaactc agttgcaaca gcagcattta
     1801 gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt cacagccggg ggagcgaaat
     1861 cccgcccggc agcaaatgct gcccaacacc aacggcggga tgttaaacag caaccgtaat
     1921 ccggatagtt cgttgaatca gcagcatatg ctgccggaaa ggagaaacgg cgacatgctg
     1981 aatcagccca gcacgccgca gcctgacatt ccgttgaaaa ctattgggcc gtaaagttcg
     2041 gaccaatcac gtcaatcgca tcggtacaga tgcaatccac gccccagcgc agcaactctg
     2101 ccgcgcgctg gggtttattg acggtataaa ccagaatccg taatccggcg tctttcaact
     2161 gcatcactcg cgctttatcg agtaacttat gattgagatg aatagagacg cagcccagcc
     2221 gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag caataaacca cgcggcagtt
     2281 ccggtgccgc ctgttgtgca gcttctaaag catcaatctc aaacgatgac agcagcggcg
     2341 gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac cattttgccc gttaatggtc
     2401 cagtgccagt ggtgggtttg atttcgatat tcgccatcat cccgtgttcg cgacagcgtt
     2461 ccgccacctg cgaaagcaac ggcagcggct cacctttaaa cgctttgctg taccaactgc
     2521 ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc cgcgacgccc cagccgttgc
     2581 tggtgcgttc gagattgtcg tcatggagca ggaagatctc gccatctttc gataacttcg
     2641 cgtcaaattc gatcatctta tgaccgtatt ttgccccgac gtcgattgcc gccagggtgt
     2701 tttccggggc cagcttaccg ccgccacgat gagcgacgat gcggggataa ggccagttac
     2761 tcatactcgt tgtcctgttt caccatcaaa aagatgcagc tgattttccg ccaaatgcag
     2821 ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc agtcgcacca ccagcttctg
     2881 ctcgccccag cgtccgtgcg ccaggttatc tgcgccgagg atctccagcg tgtccatcac
     2941 cagcggcaca ccaccttctg cctgcgagct tagcgctata tgttccgggc gaatgccgag
     3001 agtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc
     3061 gtccagttcg aaatgcgtgc cttcattatt cacgcggcct gtcagcaggt tcatcgccgg
     3121 gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt caactggtgt
     3181 gccaatctgt tcggcaacgc caccgttcat caccattact cgctgggcga gcgtcatcgc
     3241 ttcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcaactgttg
     3301 cagttcaaga cgcatctgca cgcgcagctt ggcatcgagg ttagagagcg gctcatcaaa
     3361 caagaacacc gccggatcgc gcacaatcgc gcggcccatc gccacacgct ggcgctgacc
     3421 gccggatagc tcacgcggac gacgcttaag cagaccgtcc agttccagaa tgcgcgccgc
     3481 ttctttaacg cgctcggcaa tttgctgctt gcccatgccg cgaattttca gcccccacgc
     3541 catgttctcc tctacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat
     3601 cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc
     3661 tgtcacccgc tccagcccgg caaccatgcg cagcagcgtc gatttcccgc agccagacgg
     3721 cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgacctg
     3781 ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct
     3841 cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc accaccggag
     3901 ggataagcgt taacaacatc gccgccatta ccgagttcca ttccgtggtg ccttcgcctg
     3961 tagcgatcat ccctttgatc cctgccacgg tggtgccgag atcaacgtcg gtaataatca
     4021 acaacggcca caaatactga ttccagccgt agataaaggt gatcacaaac agcgccgcca
     4081 gattagtttt ggacaacgga aaaacgatgt cgcaaaagaa acgcattggt gacgcgccgt
     4141 cgatccgcgc ggcttccacc aattcatccg gtagggtcat aaagaactgg cggaacagaa
     4201 acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcattt
     4261 tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg
     4321 tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa
     4381 atgccgagag catcgagacg gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc
     4441 tgttaagcaa catccgccag aacggcgcgc tattcgtgcc taccccgtta acccagatgt
     4501 tgtggatatt ttccagcaga tgtgtgccgg ggatgagcgt catcggcgcg gcatagacgg
     4561 cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga
     4621 tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc tcaatcattg
     4681 gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa
     4741 catcaacacc accgactgcg cggcagacga agccagatcc agtccggtaa agccttcgcg
     4801 gtagatctta taaatcagcg tagtggtggc ctgaaccggc ccgccggacg tggcggcgtc
     4861 gatcaccggg aaggtgtcga agaaggcgta caccaggttc accaccagca ggaagaaact
     4921 caccggagcg ataagcggca gagcaatctt aaagaagcgg cgaatcggcc ctgcgccgtc
     4981 aatagctgcg gcttcaatca acgaacgggg aatggagtgc agcgcggcat agaagaacag
     5041 aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca ttgcctgacc
     5101 gctgttttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc
     5161 gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata
     5221 cggcagcagc atcaaggttt gatagaaacg gctgccgcgc acgatgtact ccaccagcgc
     5281 cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt
     5341 cgtccagaag gagtctagat agtagctgtc atggaacagc gtgacgaagt tatccagacc
     5401 gacaaactgg ctggagaaac caaacggatc gacgctttgc cgcgagtacc acaacgcttc
     5461 gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata
     5521 aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac
     5581 tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac
     5641 gcatccgaca ttacactgat taagacttcg tcgatttctc aaagcggcgc agcaactgat
     5701 ttccacgctc aacggcggta tccagtgcct gctgtggtgt cttcttaccg gtccacacgc
     5761 tctccagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgcagccctt
     5821 tggtgaacgg caacggcggc ttattcagca tctgacgcgt cgcggtatcc gcccctgggt
     5881 ttttctcata aaagccctgc tcacgggtca ggtcatacgc tgctttggtg attggcagat
     5941 aaccggtttt ctgatgccac tcggcagcgt tttctggctt cgcgaggaag tcgaggaact
     6001 tcgccacgcc ggtatacgtt tctttatttt tgccctgcat cacccacagg ctggccccgc
     6061 cgataatggc gttttgcggt gcatctttcg catcggcgtc gtaaggcatc atgcctacgc
     6121 catagttaaa tttggcgtac tcacgaatat tagcgagaga accggaagag gcggtggtca
     6181 tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga
     6241 agtcgccctt cttgttcatc tcttcgagca tggcaatgtg tttcacctgc tccggcttgt
     6301 tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac
     6361 cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc
     6421 acttcatacc ggaggctttc agttttgcgg aagagtccgc cagatcctgc caggttttcg
     6481 gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg
     6541 gagtcgagct gttgaatggc tgggagagta agtggcccgt tttgctgtcg gagtagtaac
     6601 cggaaaccgt cggcacaaac tgcgactcat cgaattgaat ccctgcctct ttaaacacgt
     6661 catataccgg tttaatggct ttcgatgcca tcatggtggc ggtgccaact tcataaacct
     6721 gcaagtaagg tgtcagaaag atgacaaggc ggtgacggcg tgggtgaggg aaaatgggag
     6781 atggggcacg gataagcgga aaaatagaag gtctgaatca aactctacag attactcatc
     6841 gtttcatgcc ggatgcggcg taaacgcctt atccggccta caagatcgtg caaattcaat
     6901 atattgcaac tcacccagta ggcctgataa gcgcagcgca tcaggcaatt ttacatttgt
     6961 cacctgtctc aaaggagtct tttgactcct tatcaatcaa cgtgttatta cccgcctaaa
     7021 tacgcacttc tcaccgcttc attcgccagt agtgcatcac cagtatcgga gagcaccaca
     7081 tggccgtttt ccagcacgta gccgcgatcc gccagcttta gcgcctggtt ggcgttctgc
     7141 tcgacgagga agatagtcat cccctgctcg cgcagctgct cgatggtgtc gaaaatttgc
     7201 tggatgataa tcggcgcaag accaagcgat ggctcatcaa gtagcagcaa acgcgggttg
     7261 ctcatcagcg cgcgaccaat cgccagcatt tgttgctcac cgccggacat ggtgcctgcc
     7321 cgctgaacac ggcgctcatg cagacgtggg aacagttcat acacccactt tatgcgctcc
     7381 tggaactggt cgcgttcggc aaaaaagccg cccatcgcca ggttctcttc caccgtcatc
     7441 cgcgagaaga cgcgacgccc ttccgggaca atcgctaccg cttcgcgcat gattttcgcc
     7501 gtctgccagt cggtaatgtc tttatcatca aacacaattc gcccgctggt agcgcgcgga
     7561 tcgccgcata acgtgccgag caaggtggtt ttccccgccc cgttcgcgcc aatgagggtg
     7621 acaatctcgc cctgattgat atgcaggctc acctcatgca gcgcctggat tttgccgtag
     7681 tgggcgctga ctttgtcaaa ggacaacatg actttttcca tcttatgcct cacctaaata
     7741 ggcacggatc acgtccgggt tattacggat ttgctccggc gtaccgtttg ccagtggcgt
     7801 cccctgattg accacataaa ttcggtctga aatccccatc accagcttca tatcgtgttc
     7861 aatcaacaag atagtggtgt tgtgatgatt gcgcagttcg gcaatcagct catccagctc
     7921 tttggtttct tttgggttaa gacctgccgc cggttcgtcg agcattaaaa tctccggctg
     7981 cgtcaccatg cagcgggcaa tctccagacg gcgttggtca ccataggcca ggttactcgc
     8041 ctgacggttg gcgtgttcca gcaaaccaat gcgctcaagc caggtggcgg cgcggtcgag
     8101 cgcttcgctc tgggcgcgac ggaaagaagg cgttttcaac aggccagaga acagcccggt
     8161 tttcagttgt tgatgctgcg ccaccagcag gttttcaatt accgtcattt cacggaacag
     8221 acgcacatgc tggaaggtgc gcaccacgcc catgcgggca atttgctgcc ccggtaaacc
     8281 ttccaggtgc tgattgcgca gtaaaatggt gccgccggtg ggtttgtaga atccggtcag
     8341 acagttaaaa accgtagttt ttccggcacc gttagggccg attaacgaga cgatctcctg
     8401 cgggtacagt tcaagattga cgttgttcac cgccagcagg ccgccgaagc gcatcatcag
     8461 gccgttcaca gataataatg gctgactcat gcctgctctc ctttcgctgc gccgtttttc
     8521 agcttcagtt gcgggcgcgt catgggcagc aagccctgcg gacgccagat catcatcagc
     8581 accatcaaac caccgagcat taacatgctg tattcgttga aatcacgcat caattcgcgc
     8641 gataccacca gcaaaattgc cgccagaatc accgcaaact gcgatcccat accgccgagc
     8701 accactatcg ccagtacaaa cgccgattca gcaaaggtga aggattccgg gctgacaaag
     8761 ccctggcgcg cagcaaacag cgttccggca aaaccggcaa acgcggcact gatggtaaag
     8821 gcagtcagct tgatacgacg cgggcttaag cccagcgaac ggcaggcgat ctcatcttca
     8881 cgcaacgctt cccacgcacg ccccagcggc atccgcagca ggcggttaat gacaaacagg
     8941 cttagcacca ccagcagcaa cgccaccaga tacaggaaga tgacgcgatc ggagggatcg
     9001 tatttcaggc caaagaaatt actgaacgtg tcccaaccgc cttcacgggc ggtacggctg
     9061 aactcaaggc cgaagaaagt cggtttcggg atctgactga taccgttcgg gccgccggta
     9121 atttcggtgt tattgagcag caatatgcgc acaatttcgc cgaaaccgag ggtaacgatc
     9181 gccagataat caccgcgcag acgcaacacc ggaaaaccaa gcaggaagcc cgccgccgct
     9241 gccatcagtc cggcaatcgg caaacaggtc cagaacccca gaccgtaata gtgattgagc
     9301 agcgcaaaag tgtaagcgcc gatggcgtaa aaaccgccgt accccagcac cagcagacca
     9361 gaaagaccaa caaccacatt cagcccgaga ccgaggataa tgtagatcat ggtcagagtg
     9421 gcgatatcca ccgtcccacg tgacaccata aacggccacg acaccgcaag caccagcagc
     9481 gccacgagga acagtttctg cttcaccgtg gagccatcaa tcgcgggcag aataaacttc
     9541 ggcccggaaa cgctttttag ccctttctgg aaagcaggtc gcaaaagctg gaagaaaaag
     9601 accaccgccg tgccgataaa cacccactgc caacggacat ccgaagcgct gtcgaccacc
     9661 agtttggtgc catccagctc cagttgcacg cccataaaga cgccagcgag gacaaagaac
     9721 atcgcggcag agagcagtgc cattgcaata tgcatcggtt tcatactttc tctacctccg
     9781 ggcgacccag aataccggtc ggcatcacca gcagcaccag aatcagcagg gcgaatgaga
     9841 ccacatcttt atattccgta ctcagatagg cagaagagag cgcctccgca atccccagga
     9901 tcagcccgcc aatcatcgcc cctggaatgc tgccaatccc accgagcacc gcagcggtaa
     9961 aggctttcat cccggccata aagccgatat aggggttaat gacgccgtag aactgaccga
    10021 gcagtacgcc agccactgcc gccatcgccg cgccaataac aaaggtcaaa gcaatcaccc
    10081 ggtcggtgtt gatgccaagc aggctcgcca ttttcagatc ttccgcgcag gcacggcagg
    10141 cgcgccccat acgggaataa cgaatgaaaa tcgtcagcgc cagcatggcg aggaaagtaa
    10201 caatccagat cactgcctgc atggtggtaa tagaggcaga gaagttttcg ctatgcccca
    10261 ccacccactg accgttaaac agactgggca gcgccacgtc acgcgaacct tcggtcaggc
    10321 taacgtagtt ttgcaggaag atggacatgc cgattgccga gatgagcgca atcaggcgct
    10381 tagagttacg caccgggcga taggctaccc gttcgatact ccagccgtag gcgctggcaa
    10441 tgacgattgc gccgacgaat cccgccgcca ccagcagcca gccggtatca atgcccatca
    10501 tcatcagtgc ggcgatgatc ataaaggaga catagctacc aatcatgtaa acctcgccgt
    10561 gggcgaagtt gatcatgccg ataatgccgt aaaccatggt gtagccgatg gctatcagcg
    10621 cgtaggtact gcccagcgtg acgccgttaa acatctgctg caagaaatat aaaaactgct
    10681 cagacataag gtaacctttc taaacccgcc cgcatttcac gggcggtggg atgatcactt
    10741 ggctgccgtg gatgaaccat cggcgtgcca ctggaagaca ccaaaatcaa atcccttaag
    10801 atcgcctttt tcatcccagt tcagcggccc aatcacggtg tttgcaccgt tagcttttaa
    10861 atctttcacc agcgccagcg gctcatcgct gccagtacgc tcaagggcag tcgccagaga
    10921 ttgcaccgcc gcgtaggtga tccagacata aggcccggac ggatctttct tgtctgcttt
    10981 cagcgcatca acgatgccct ggtttgccgg atcctggtca tagcgttttg gcatagtgac
    11041 caacatgcct tcggcagcat cgccagcaat attcgacaat gatgcgttac ccacaccttc
    11101 cggccccata aactgagttt tcaggccaac ggaacgggcc tggcgcagca tctgccccat
    11161 ttccgggtag taaccgccgt agtaaacgaa gtcgatgttt tcttttttca ggcgggcgat
    11221 cagcgcggag aaatctttct ccccggcggt aataccgtcg aagaagacga cgttggcgtt
    11281 agccgctttc agcccgtcct gcaccgaacg cgccagccct tcgccatact gttgtttgtc
    11341 gtgaatgatg gcgatgcgct ggggcttcac cgtctcaaga atgtattttg ccgccgttgg
    11401 cccctgggaa gagtccagcc cggcagtacg cataatgtgt tgataaccgc gttgggtcag
    11461 ctccgggttg gtcgctcccg gcgagatcat cagaatacct tcgtcttcgt agatatctga
    11521 tgcaggttgg gtagaagaag aacacagatg accaataacg tatttaatgc cgtcattaac
    11581 gattttgttg gcgaccgcaa cggcttgttt cgggtcgcag gcgtcgtcat attccacgcc
    11641 aaccagcttg tcgcccttaa ttcccccttt ggcattaatg tctttaatcg cctgacgcgc
    11701 gccgttaaat tccatatcgc cccactgggc aatcgggccg gacatcgcgc cgacaacggc
    11761 gactttaata tcgtcagcca tagcggtgtg tgaaattgcc agtgcaatca tccctgcgat
    11821 gatagttttc gcattccgtt tcatagtcaa aaatccccat tcgtgatgtt gtgttgcttt
    11881 gtttttatgt gttaacaaat cagactgttc tttttttata ctgcactgtt tttgcctgtc
    11941 tgattaaggg tttagcgcag tattttgtga taacagtgat taaaatccct atttttcagt
    12001 cgattaagaa cagataatat tctgaattta ttgatagata aacagaaaaa agtgcctttg
    12061 tcagcataaa ataacggcac aaagggcgga ataattcact accattcagg ggattatgct
    12121 gggtattttt cattctctaa tgttttaact ttgtaattat tgctgttaaa aaattaatca
    12181 cctgccaaaa gaaataaaaa agagaaagcc tccgattaaa ttatttcgct acactggttc
    12241 cacttttgtg atttacacgg gttacccatg aagctgacca tcattcgatt agaaaacttt
    12301 agcgaccagg accggattga cctgcaaaag atctggccgg agtattcccc ttcctcttta
    12361 caggttgacg ataaccaccg tatctacgcc gcgcgtttta acgagcgcct gctcgctgcc
    12421 gtacgggtaa ccttaagcgg caccgaggga gcactggatt ccctgcgtgt gcgggaagtc
    12481 acccgacgtc gcggtgtggg gcaatatctg ctggaagagg ttttgcgtaa caatcctggc
    12541 gtttcatgct ggtggatagc ggatgcaggc gtggaagatc gcggtgtgat gacagcgttt
    12601 atgcaggcac tgggatttac ggcgcaacag ggcggctggg agaagcgtta atcgtcaagt
    12661 tgatttcaaa agtgatgacc aacgagaaat tgcctgatgc gctacgctta tcaggcctac
    12721 aatgtgtgtt gcaatttact gatttctttg gatcttatag gccggataag gcgtttacgc
    12781 cgcatccggc atgaagcaac gtactcgata ttagcaattt ggcggcaacc caaagttgcc
    12841 gattaatgat tacttcgcat ctgtcgccgt gccgttggcg tgccagtcaa atacgccgaa
    12901 ctcaaagcct ttcagatcgc ctttctcatc ccacgtcagc ggtcccatta cggtatccac
    12961 ggagttcgct ttcaggtatt tggcgatttc agccggatcg tcagactgat tcaggcccgc
    13021 ctgcaaagat tgcagcgcgg cgtaggtggt ccaaacgaat gcgccgcttg ggtcctgttt
    13081 tttcgctttg atcgcgtcaa caatgggttt gtttgccgga acctgatcgt agttcttcgg
    13141 cttggtcacc agcagccctt ccgctgattc gcccgcaatg ttagacagcg aaacgttagc
    13201 cacaccttcc ggccccataa actgagtttt cagccctgcc gcgcgtgcct gacgcaggat
    13261 ttgccccatt tccgggtggt aaccgccgta gtaaacgaag tcgatattct ctttcttcag
    13321 acgcgccacc agcgtagaga aatctttttc cccggcggtg ataccatcaa agaacaccac
    13381 gttagcattg cctttcttca ggccgtcctg caccgctcgt gccagacctt cgccgtattg
    13441 ctgtttgtcg tgaacgatag caatacgctg cggtttcact ttctcaagaa tatatttcgc
    13501 cgccgtcggc ccctggtcgg agtccaggcc ggtggtgcgc aggatcagct gatagccacg
    13561 ggcggtcagc tccggcgcgg ttgccgctgg ggtgatcatt aaaatgcctt cgtcttcgta
    13621 gatgtcagac gcaggctgcg ttgatgaaga acagaggtga ccaatcacat atttaatgcc
    13681 gtcgttaacg actttgttcg ccaccgcaac cgcctgtttc gggtcacagg catcgtcata
    13741 ttttacgatt tgcagtttgt tgcctttaat gccgccttta gcgttgatat ccgcaaccgc
    13801 ctgctctgcg ccggtaaact cttggtcacc gtactgcgca accggaccgg acattgcgcc
    13861 cacgaccgcg actttaatat cttctgccag agccatattg ctgaatgcca gcgcgataca
    13921 tcctgccagt aacgctttac cctttatgtt catcctgaga atccccattc ttctggttat
    13981 tacgtgtgtt gtgatgttgt tgttcagcac tttatttcgt tttatgcatg gctacccgtg
    14041 ctttagcagc atactctgct aaaacatacc cgatttttat gatattggaa tagctatttt
    14101 gacagtttat taacaatctg cgtggggatt ggcgttttgc cggaggggaa attgattatt
    14161 gtagaggccc aaaaaacaaa aaccccggac tcgcatccag ggttctctgc ttaatagcgg
    14221 aaattacgct tctatagcag cgcgcagttt tttcatcgcg ttcttttcca gctggcgtac
    14281 acgttcagcg gaaacaccgt aacggtcagc cagttcctgc aacgtggact tgttgtcttc
    14341 gtccagccag cgcgcgcgga tgatgtcctg gctgcgttcg tccagacctt gcatcgcgtc
    14401 ggtcagacgg tttgccgcct gctcttccca gttatcatct tcgatgccgt cggcaaagtt
    14461 agatgattta tcctgcagat agagcaccgg tgccatcggc tggctgtcgg aatcgtcgtc
    14521 ggaagacagg tcaaaggtca tgtcctgtgc cgccatacgt gattccatct cacgaacgtc
    14581 cttgctggtt acgcccagtt cacgggccac catttcgact tcatcctggt taaaccagcc
    14641 cagacgctgc ttggttttac gcaggttgaa gaacagtttg cgctgcgctt tggtggtcgc
    14701 aactttgacg atacgccagt tacgcagaac gtattcgtgg atctctgctt tgatccagtg
    14761 aacggcgaag gagaccaggc gcacacccac ttccgggttg aaacggcgca ctgctttcat
    14821 caggccgatg ttaccttcct gaatcaaatc cgcctgtggc aggccatagc ccgcataatt
    14881 acgagcaata tgaacaacaa accgcaggtg agacaggatc agcgttttag ctgcttccag
    14941 atcgccatgg taatgcagct tttcagccag cgcccgctcc tcgtcagccg acaacatcgg
    15001 ccacgcgtta gctgcccgga tgtaggaatc caggttgcca actggggcta aagctaaact
    15061 ttgcattttg tcagtcattc aaatcctctc aatcgatatc ttctggcgct tcagtggtag
    15121 caacaactgt gccagagctt aagagcaacg aggttatcat ccactgtttt atcagacagt
    15181 gattttatcc acaagttcaa tgcaagcttg tgaataaatt acgcacaaaa tgtgacatag
    15241 agatgaaata ccgggaagag acaacgggat ctctttccct gctacggaac ccattgcagg
    15301 gaaagagtat aacacgcttt tattattcag gcgtaaagtg gcgtaaatgt tgtaccgtgg
    15361 caagccacgc tgccacccag ccaatcatcg agcataccag cagcaatagc aggcattcat
    15421 cgaacgataa accattgata tcaaacttcg ttccgaaaac ctgtgccact tccgcaaccg
    15481 ccgatgacaa tcgcagcacc agaatttctg acaaaattaa tgacaacaat gcgccagaaa
    15541 atcccagcag tgcgccacca tacaggaacg ggcgaaggat gaaaccatcc gtcgcaccaa
    15601 tcagtttctg cacgttgatg gagtcacggc gagcaaagat gctcagacgt acgctgttgc
    15661 cgatgacgag gaacactgcc gccaccatca acacgccgat catcgccgaa acgcgcccga
    15721 ccagcccggt caacgccgcc agacgggcaa accagctgtc atccatccgc acttcgtcaa
    15781 tgccgttaat ctgcgtgata cgatcacgca gcgtattgag tgattccgtc ccctgaaaat
    15841 cgagtttcgg gatcaccacc gccactgccg gaagcgggtt ttcttccagc atatccagcg
    15901 caccaccaaa accagaccag ttacggaact cgcccagtgc gtcttcacga gaaagatagt
    15961 tcactttctc cacgccttgc tcggcctgca actgtgccac cacgcccgca gcggcgtcat
    16021 cgtccagcgt tttttgcaag taaacagtga tttgcggtga cggataatac tgcgtcgccg
    16081 cctgattaac gtttttgtac accatataac agacgctggg cagcgtcagg gagatggcga
    16141 taaccatcac cgttaaaaac gtggcgaacg gtttgctttt cagatcctgc aatgcgccgt
    16201 ggaaggcata gcgcacctgt tcgttgaaaa cgttggtttt gcgatttacc ggttttggcg
    16261 aggatttcgc gcgtttcggt gcgttacgac cgccgtcgcc tgagccgccg accgatttac
    16321 ggaagcgatc cagacgccca ccaaactgcc gaatatgatt gattgcatcg cgcttattca
    16381 tggcccacgc ctccatgcaa gtgaccatcg ctcagggtga gcatgcgatg ggaacgccgt
    16441 gaaatcaggt tgatgtcgtg cgttgccatc aatacggtta ccccaacgcg gttaaactct
    16501 tcaaacagac gtagaatccc ttccgacaac gcgtcgtcca ggttaccggt cggttcgtcc
    16561 gccagcagta ccgcgggctt attcaccacc gcgcgggcaa tgccaacacg ctgttgttca
    16621 ccgccggaaa gctgaatagg gaagttcttc gctttgtcca gtagcccgac tttatccagt
    16681 gccgccgaca cccggcgacg aatatcgtca ccgctggcac cagcgataat cagcgggatc
    16741 gccacgttat cgtagacagt acggtccatc agtagatggt gatcctggaa aatcatgcca
    16801 atctggcggc gcagaaacgg aacttcacgg tttttcagac gcgtgatgtc atggccgcta
    16861 aaccagattt tcccggcgct gggccgctca atcccacaga tcagcttcag gagggtactt
    16921 ttccctgcgc cggaatgacc ggtcagaaac gccatctcac ccggctgcat atggaacgta
    16981 acgccctgca gcgcctgtct cccaccgaga taagccttgc tgacatgttc aaagcgaatc
    17041 attgttaatc ctctcgggca aaaagtgcct ctataaagtc gtccgcctta aacggacgca
    17101 aatcctcaat acgttcgccg acaccaatgt agcggatagg gataccaaac tggtcagcca
    17161 ccgagaaaat taccccgcct ttcgccgtgc cgtccagttt cgttagcgtg atgccggtta
    17221 agccaacagc ttcgtggaac agtttggcct ggcttaccgc gttctgcccg gtgctggcat
    17281 caatagtcag cataacttca tgcggcgctt caacgtcgag tttcttcatc acgcggacga
    17341 ttttcttcaa ctcttccatc aggtgcgatt tgttctgcag gcgtccggcg gtatcggcaa
    17401 tcaggacgtc gatattacgc gctttagctg cctgaatggc gtcgaagata acagaggcgg
    17461 aatccgcccc ggtatgctgg gcaatcaccg gaatattgtt gcgctgaccc cagacctgaa
    17521 gctgttcaac cgctgctgca cggaaagtat cgcccgccgc cagcatcacc gatttaccct
    17581 gctgctcaaa ctgacgtgcc agcttaccaa tcgtcgtggt tttacccaca ccgttgacgc
    17641 ccaccatcag gatcacgaac ggcgttttac cttcaacatt cagcggctca tcgactttcg
    17701 ccagaatctc acccatctct tctttcagca ggccatagag cgcctcggca tcacgaagct
    17761 gcttgcggga tgcgccttcc gtcagattgg tgataatttt acgtgtggtt tccacgccca
    17821 catcggcgat caaaagctgc tcttccagct cctcaaacag atcatcgtcg atttttttac
    17881 cgcggaacag gctgataaat ccggaaccga gattttcttt ggtttttaac aggctgcgtt
    17941 tcaggcgcgc gaagaaacct tctttggttg gtttttcctg ctcctgagcg atttcttcca
    18001 ccggctgctc ttcttctgcc ggaggaacca ccatcaccgc ctcttctgct gcttcggctg
    18061 ccagcgcctg tgcttccagc tcttcgtcgg tgatttcttc tttagccgct tcttcttccg
    18121 ccgctttgac aatctctacg gtttccgctt cagcctgcca ctcttctggc gaaacctctt
    18181 cggcgttgac gtcttccggc aacggcagct cttcacgttc gatagccact ggctccggcg
    18241 tttcttctac gaccggttcc ggctgtgcaa caacttccgc ttcaggctgc gctttttcac
    18301 tttcaacaac ctgttcagtg acttccacaa cgtcggcagc aaaagtttcc gcctcggctt
    18361 cagtatgcgc ctgcggctgc tcttcaacgg cttgttccga agccttcaca ggctcttgcg
    18421 cctgaacgat ttcttctaca accggttgtt cattctgaac ttctgtctct ttttccgggg
    18481 tctgctcttt ttgaccaaag cccagccagg aaaaaaagcc acgttttttt tctttcgcca
    18541 tttgcgacta cactcctcgc tgttgcttca tggcacagcg ttaacgctat gtacatagca
    18601 gctaaaaaaa tgatgaaata gtctatcact taacttaatt cacatcaccg cctgcaagta
    18661 tgtgttatct ggcggattga gcaatttatc atgaaaaaac cgaatcattc cggcagcggc
    18721 caaatccgca ttattggcgg gcagtggcga ggccgtaaac tcccggttcc tgatagccca
    18781 ggtctgcgcc ccaccaccga ccgcgtacgc gaaacgttgt ttaactggct ggctccggtc
    18841 attgttgacg cccaatgtct ggattgcttc gccgggagcg gcgcgctggg gctggaagcg
    18901 ttatcgcgct acgctgcggg ggccacgtta attgagatgg atcgtgcagt ttctcagcag
    18961 ttaattaaga atctggcgac actaaaagca ggcaatgcac gcgtggtgaa cagcaacgcg
    19021 atgtcattcc tggcgcaaaa aggcacaccg cataatatcg tgtttgtcga tccaccgttc
    19081 cgccgtggct tgttagaaga gacgataaat ttactggaag ataacggctg gctggctgac
    19141 gaagccctga tttatgtcga aagcgaagtc gaaaacggtc tgcccaccgt tccggcaaac
    19201 tggtcgttac atcgggaaaa agtggcgggt caggtggctt atcggctgta tcaacgcgaa
    19261 gcacaaggag aaagtgatgc tgattaatat tggtcgtttg ttaatgctct gcgtttgggg
    19321 atttttaatc ctcaatctgg tgcatccctt cccgcgcccg ctaaatattt tcgttaacgt
    19381 ggcgctgatt tttaccgtgc tgatgcatgg tatgcagctg gcgctattga aatccacttt
    19441 accgaaagat ggcccgcaga tgaccaccgc cgaaaaggta cggattttcc ttttcggcgt
    19501 gtttgaactg ctggtctggc agaagaaatt taaagttaaa aaataacctt attgttcgcc
    19561 aacaaagcta acaaagcgcg ttcctttata gctcagcgta cctttatcgc ccactgtcag
    19621 ggcgtggtac tgctgggcgt cgaggcgaaa cgtctgctcc attcctccgc tttgcggttt
    19681 gaagcttgcc tcgtagcgca tactggtgcc tgccggagtc acttcctgct ggcgcgaacg
    19741 gcgatcgtta atcggttttt cccgcttgtt gctcaccacc accagctttt gctggagcgg
    19801 agccatatca ttatcagctt tttcccgtcg ctgttgcata aaacgaaacg atgcggcgac
    19861 gacaattaag ccaatgataa caataaagaa aagaggtggt ttgctcatct ttatccctca
    19921 tcggaaaatg cggaaataag cataccctgc cagttatggt gttgtcatcc gtccaccctc
    19981 gccactaaac tggaagcaag accgtaggca ttccgcttac gaaaaaataa cgaattcaag
    20041 gaactaagat gctttggtcg tttatcgctg tctgtctttc cgcatggcta tctgtggatg
    20101 catcgtatcg tgggccaacc tggcaacgct gggtgtttaa accgttaacc cttcttctcc
    20161 tgctgttact ggcctggcaa gcgccgatgt tcgacgccat tagctatctg gtgctggcag
    20221 ggctgtgcgc ctcactgctg ggcgatgcgc taaccctgtt gccacgtcaa cgtctgatgt
    20281 acgccatcgg cgcgtttttc ctctcgcacc tgctgtacac catctatttc gccagtcaga
    20341 tgacgctctc tttcttctgg cctctgccac tggtgttgct ggttctgggt gcgctgttac
    20401 tggcgattat ctggacgcgg ctggaagagt atcgttggcc tatctgcacg tttatcggca
    20461 tgacgctggt gatggtgtgg ctggcaggtg aactgtggtt cttccgtccg accgctccgg
    20521 cgctctctgc gtttgttggc gcttcgttgc tgtttatcag taactttgtc tggctgggga
    20581 gccactatcg ccgacgcttc cgtgcggata acgcgattgc tgcggcctgc tactttgccg
    20641 gtcacttcct catcatccgc tcactgtatc tctgataaaa cttgactctg gagtcgactc
    20701 cagagtgtat ccttcggtta atgagaaaaa acttaaccgg agtatgccat gtcgacacct
    20761 gacaatcacg gcaagaaagc ccctcaattt gctgcgttca aaccgctaac cacggtacag
    20821 aacgccaacg actgttgctg cgacggcgca tgctccagct cgccaacgct ctctgaaaac
    20881 gtctccggca cccgctatag ctggaaagtc agtggcatgg actgcgccgc ctgtgcgcgc
    20941 aaggtagaaa atgccgtgcg ccagcttgca ggtgtgaatc aggtgcaggt gttgttcgcc
    21001 accgaaaaac tggtggttga tgccgacaat gacattcgtg cacaagttga atctgcggtg
    21061 caaaaagcgg gctattccct gcgcgatgag caggccgccg acgagccaca agcatcgcgc
    21121 ctgaaagaga atctgccgct gattacacta atcgtgatga tggcaatcag ctggggtctg
    21181 gagcagttca atcatccgtt cgggcaactg gcgtttatcg cgaccacgct ggttgggctg
    21241 tacccgattg ctcgtcaggc attacggctg atcaaatccg gcagctactt cgccattgaa
    21301 accttaatga gcgtagccgc tattggtgca ctgtttattg gcgcaacggc tgaagctgcg
    21361 atggtgttgc tgctgttttt gattggtgaa cgactggaag gctgggccgc cagccgcgcg
    21421 cgtcagggcg ttagcgcgtt aatggcgctg aaaccagaaa ccgccacgcg cctgcgtaac
    21481 ggtgagcggg aagaggtggc gattaacagc ctgcggcctg gcgatgtgat tgaagtcgcc
    21541 gcaggtgggc gtttgcctgc cgacggtaaa ctgctctcac cgtttgccag ttttgatgaa
    21601 agcgccctga ccggagaatc cattccggtg gagcgcgcga cgggcgataa agttcctgca
    21661 ggagccacca gcgtagaccg tctggtaacg ctggaagtgc tgtcagaacc gggtgccagc
    21721 gccattgacc ggattctgaa actgatcgaa gaagccgaag agcgtcgcgc ccccattgag
    21781 cggtttatcg accgtttcag ccgtatctat acgcccgcga ttatggccgt cgctctgctg
    21841 gtgacgctgg tgccaccgct gctgtttgcc gccagctggc aggagtggat ttataaaggg
    21901 ctgacgctgc tgctgattgg ctgcccgtgt gcgttagtta tctcaacgcc tgcggcgatt
    21961 acctccgggc tggcggcggc agcgcgtcgt ggggcgttga ttaaaggcgg agcggcgctg
    22021 gaacaactag gtcgtgttac tcaggtggcg tttgataaaa ccggtacgct gaccgtcggt
    22081 aaaccgcgcg ttaccgcgat tcatccggca acgggtatta gtgaatctga actgctgaca
    22141 ctggcggcgg cggtcgagca aggcgcgacg catccactgg cgcaagccat cgtacgcgaa
    22201 gcacaggttg ctgaactcgc cattcccacc gccgaatcac agcgggcgct ggtcgggtct
    22261 ggcattgaag cgcaggttaa cggtgagcgc gtattgattt gcgctgccgg gaaacatccc
    22321 gctgatgcat ttgctggttt gattaatgaa ctggaaagcg ccgggcaaac ggttgtgctg
    22381 gtagtacgta atgatgacgt gctgggtatc attgcattgc aggataccct gcgcgccgat
    22441 gctgcaactg ccatcagtga actgaacgcg ctgggggtca aaggagtgat cctcaccggc
    22501 gataatccac gtgcagcggc ggcaatcgcc ggggagctgg ggctggagtt taaagcgggc
    22561 ctattgccgg aagataaagt caaagcggtg accaagctga atcaacatgc gcccctggcg
    22621 atggtcggtg atggtattaa cgacgcgcca gcgatgaaag ctgccgccat tgggattgca
    22681 atgggcagtg gcacagacgt ggcgctggaa accgctgatg cagcattaac ccataaccac
    22741 ctgcgcggcc tggtgcaaat gattgaactg gcacgcgcca ctcacgccaa tatccgccag
    22801 aacatcacca ttgcgctggg gctgaaaggg atcttcctcg tcaccacgct gttagggatg
    22861 accggcctgt ggctggcggt gctggcagat acgggtgcga cggtgctggt gacagcgaat
    22921 gcgttaagat tgttgcgcag gagataaggc aaaccgatcg caacattgag cgcgatcggt
    22981 cccctcgccc ctctggggag agggttaggg tgaggggaaa aggcggcatc gaagccaatc
    23041 tttccccatc aaccgccttt acgaatcaaa taacgataag gcagtccatc cgtctctttc
    23101 gcaaccagtt cgtgttccat aaaggtacaa aacccaggaa tatcgcgggt agtggccgga
    23161 tcgtcggcga taatcagcaa cgtttcgcca ggctgcatat tgcgcacagt tttgcgcacc
    23221 atcattacgg gttccgggca gcgcaggcca agtgcgtcga gtgtgtggtc agggctggaa
    23281 aagagatcgg tcattttctt ctcatcacat aaaaaaacgg cgctagttta cgccctgtga
    23341 gtccgtaagc aaaccaggtt aacgattgcg tgaaaattaa ccattgcatt gtcaacgtaa
    23401 agcagtatca tgcggcggct cgaaaaaagg gtaagcacgt tattatgtta aggtaacaga
    23461 cgtgtcgtac gtattgggtt ccctcacccc aatggttaat caaaaaggta caatatgaac
    23521 gttttctcgc aaactcaacg ctataaggcg ttgttctggt tatcgttatt tcatctgctg
    23581 gtgatcacct ccagtaacta tctggttcag cttcccgtct ccattttggg tttccatacc
    23641 acctggggcg cgtttagctt cccgtttatt ttccttgcta ccgacctgac cgtgcgtatt
    23701 tttggcgcac cgctggcccg tcgtatcatc tttgcggtga tgatcccggc gttgctgatc
    23761 tcctacgtca tctcgtcgct attctatatg ggttcctggc agggattcgg cgcactcgcc
    23821 cacttcaacc tgtttgtcgc ccgtatcgcc accgccagtt tcatggccta cgcgctggga
    23881 cagatcctcg acgtgcacgt ttttaaccgc ctgcgtcaga gtcgccgctg gtggctggca
    23941 ccgacagcgt ccacactgtt cggtaacgtc agcgacacgc tggccttttt cttcattgcc
    24001 ttctggcgta gcccggatgc ctttatggct gaacactgga tggaaatcgc gctggtcgat
    24061 tactgtttca aagtgttaat cagtatcgtt ttcttcctgc caatgtatgg cgtattactc
    24121 aatattctgt tgaaaagact ggcagataaa tccgaaatca acgctttgca ggcgagttaa
    24181 aggttcgtta tcagagttgt gataagatgg atgaatgagc cgttatggcc gtttatcgaa
    24241 aggaagaagt caatgcgcaa tctggttaaa tatgtcggaa ttggcctgct ggttatgggg
    24301 cttgcggcct gtgatgataa agacactaac gctacggcgc agggttcggt cgcggaaagt
    24361 aacgctaccg ggaatcccgt caacctgctt gatggcaagt taagtttctc gctgccagcg
    24421 gatatgaccg accagagcgg taagctggga acgcaggcca ataacatgca tgtctggtcc
    24481 gacgccaccg ggcagaaagc agtcatcgtc atcatgggcg atgatccgaa agaagatctg
    24541 gcggtgttgg cgaagcgtct ggaagatcag caacgtagcc gcgatccgca gctgcaagtg
    24601 gtaaccaaca aagccattga gctgaaaggt cacaaaatgc agcagttaga cagtattatc
    24661 tccgcgaaag gccagacggc gtactcttcc gttattctgg gtaacgtggg taatcaactg
    24721 ctgaccatgc aaattactct gccagctgac gatcagcaaa aagcgcagac caccgcagaa
    24781 aacatcatta atacgctggt tattcagtaa gttttaagat gatgaggcgg cctcagggac
    24841 gtgttccgga ggccgttttt ttaatcgcca cgtcagtaat aacgcgattg cgaccagtcc
    24901 cgccgccgcc agataaatca ccggtacgcc cgcccagctc atcaccagcc cagccagtgg
    24961 tccggtcacg ccaagcgata aatccataaa tacggtgtaa gttgccagcg ccgccccctg
    25021 attttgctgc ggaaccgctt ttaccgccac tacgcccaat gccgggaaca ccagcgaaaa
    25081 ccctgccccc gccagtaaga cgccgacttt cgccatccac agcatagtcg ccacgccaac
    25141 cagtagcagg ccgattatct caacgctaaa gcaaatcatc gccacgttta agccgccgat
    25201 acggttaatg ccgttaggga ataacaaacg cgtaccgaca aacgcacagc taaacagcgt
    25261 cagcgcgaaa gccgcaccgt cccaaccttt agcgtcataa aacagcgtga taaaggtggc
    25321 gatgacgcca aatccggcag aagccagtgc cagtgccata ccgtacagcc agacgcgccc
    25381 aagcaccgcg cgaaacggca gcggtttgcc tttactggct tttaccgtcg gacgcgggat
    25441 cgccaacaaa atggccacca gcgccacgcc cataatgatt aacgctaacg cctgcaagcc
    25501 gccccagtga taaaacacga cgcctaacgg cgcacccatc gccatcgccc cgtaattgac
    25561 aatgccgttc cacgaaatca ctcgcccgat atgcagcgag ccaaccacgc caacgcccca
    25621 cagggtcgat cccgttccgg caaaactttg cccaatccca aggatcacgc gtcccaggca
    25681 aagtaataac aggctgatga cgggcagact ggcggttaat cctgccgtca gatatcccag
    25741 accgctcaaa aagcagccgc ataaaccgaa gacgacaatc tttttggggc ccagcaaatc
    25801 ggcgtaacgt ccggcatgag gacggctcag caaggtggcg aaatattgca ggctgataac
    25861 caatcctgcc cagaaagcgc taaagcccat cacatcatgg acatagcccg gtaatacagc
    25921 gagcggcaac ccgatggtga ggtagctggc gaagttaaac atgacaatgg agacaatgcg
    25981 caaattcagg cgcaatccgt ttagcgcggg ttcggctacg ggttcgggca tgaggatcac
    26041 cacattttta caacagtgtt tcatttttac cacgtgctga cgtgaaaatc agcagtaaga
    26101 atcagaatat tgctggcgtg actcccgcta cacttaatac aaaaagtcac aaggaagccc
    26161 caatggaaat ccctcaaccc gataaaacgg gcatgcacat tctgctcaag ctggcctcgc
    26221 tggtagtgat cctcgcgggc attcacgcag cggcagatat cattgtgcag ctgttactgg
    26281 cgctgttttt tgccatcgtc ctcaacccgc tcgttacctg gtttattcgt cggggagtac
    26341 aacgccccgt tgccattacg attgtagtgg tggtgatgct gatcgcacta accgcgctgg
    26401 tcggcgtact ggcggcatcg tttaacgaat ttatctctat gctgccgaag tttaataagg
    26461 agctgacgcg caaacttttt aaattgcagg agatgttgcc ttttcttaat ttgcatatgt
    26521 cgccggagcg aatgctgcag cggatggact cggaaaaagt ggttaccttc accacagcgc
    26581 taatgaccgg gctttccggg gcaatggcga gcgtgctttt gctggtgatg accgtagttt
    26641 ttatgctgtt tgaagtgcgc cacgtccctt acaaaatgcg ttttgcgctg aataacccac
    26701 agattcacat cgcaggatta caccgcgcac ttaaaggcgt ttcgcactat cttgcattga
    26761 agacgctact cagtttatgg acaggtgtca tcgtctggct ggggctggag ctgatggggg
    26821 tgcagtttgc gctgatgtgg gcagtactgg cgtttttgct caactacgtg cccaatatcg
    26881 gcgcggtaat ttccgccgta ccgccaatga ttcaggtgct gctgtttaat ggtgtttacg
    26941 aatgtattct ggtcggcgca ttgtttttag tggtccatat ggtcatcggc aatattttag
    27001 aaccacggat gatgggccat cgcctgggga tgtccaccat ggtggtattt ctttcattgt
    27061 taatttgggg atggctgctc ggcccggtag ggatgctact ttcggtgcca ttaaccagtg
    27121 tgtgtaaaat ctggatggaa accaccaaag gcggtagcaa actggcgatt ttactggggc
    27181 caggcagacc gaaaagtcgg ttaccgggat gaggcgacaa gtgatacgat acgcactttc
    27241 attttccatt aaacgttggc cctgatatgt atcggatagt tctggggaaa gtttcgacct
    27301 taagcgcagc tccactgcca ccgggtttac gcgagcaagc accgcaaggt ccacgacgcg
    27361 aacgctggct ggcggggcgt gcattgcttt cgcacacgct ttccccgcta ccggagatca
    27421 tctatggcga acaaggcaaa cctgcattcg cgccggaaac gccgctatgg ttcaacttaa
    27481 gccatagcgg tgacgatatc gctctgctgt tgagcgatga aggcgaagtc ggctgcgata
    27541 tcgaagtgat tcgcccgcgc gccaactggc gctggctggc gaacgccgtg ttcagcctcg
    27601 gggaacacgc tgaaatggac gccgtgcatc ctgatcagca actggaaatg ttctggcgca
    27661 tctggacgcg caaagaagcc atcgttaaac agcgtggcgg cagcgcctgg caaatcgtca
    27721 gcgtagatag cacctatcac tcctcgctgt cagtcagcca ttgtcagcta gaaaatttaa
    27781 gccttgcgat ctgcaccccc acccccttta cgctcaccgc cgacagtgtg caatggatcg
    27841 attcagttaa ctgatccgcc cacccgactg cccatctatt gatccagaac aggtaatcag
    27901 tatgacgaat acttaaaatc gtcatactta tttccgccat ctattttaat ccattggggt
    27961 taccatgctc tccacactcc gccgcactct atttgcgctg ctggcttgtg cgtcttttat
    28021 cgtccatgcc gctgcaccag atgaaatcac taccgcctgg ccggtgaatg tcgggccact
    28081 aaacccgcac ctttacacgc ctaaccagat gttcgcccag agcatggttt atgaaccatt
    28141 ggtgaaatat caggcagacg gttcggtgat cccgtggctg gcaaaaagct ggactcattc
    28201 agaagatggt aaaacctgga ccttcaccct gcgtgatgac gtgaaattct ccaacggtga
    28261 accgttcgat gccgaggcgg cggcagaaaa cttccgcgca gtgctcgata accgtcaacg
    28321 tcacgcctgg ctggagctgg caaaccagat tgttgatgtt aaagcactca gtaaaacaga
    28381 gctgcaaatt accctgaaaa gcgcctacta tcctttcctg caagaactgg ccctgccccg
    28441 tcctttccgt tttatcgctc cctcgcagtt taaaaaccat gaaaccatga acggaattaa
    28501 agcgccgatt ggcaccggac cgtggatttt gcaggaatcg aaactgaatc agtacgatgt
    28561 cttcgtccgt aacgaaaact actggggcga aaagccagcg attaaaaaga tcacctttaa
    28621 cgtcatcccg gacccgacta cccgcgcggt ggcgtttgaa actggcgata tcgacctgct
    28681 gtacggaaac gaagggttat taccgctcga taccttcgcc cgctttagcc agaatccggc
    28741 ttaccacacc caactgtcac agccgatcga aaccgtgatg ctggcgctca ataccgccaa
    28801 agcccccacc aacgagctgg cagtacgtga agctcttaat tacgcggtaa acaaaaaatc
    28861 gctgattgat aacgcgttat atggcaccca gcaggtcgcc gacaccctgt ttgccccttc
    28921 tgtgccttac gctaatctcg gcctgaaacc gcgccagtac gatccgcaga aagcgaaaga
    28981 gttgctggaa aaagccggtt ggacgctgcc tgcgggcaaa gacatccgcg agaaaaatgg
    29041 tcagccgctg cgcattgaac tgtcgttcat cggcaccgat gcgttaagca aatcgatggc
    29101 ggaaatcatt caggctgata tgcgccagat tggcgcagat gtctcgctga ttggcgaaga
    29161 agagagcagt atctatgctc gtcagcgcga cggtcgtttt ggcatgattt tccaccgcac
    29221 ctggggcgcg ccatacgatc cacacgcctt cctgagttca atgcgcgtac cgtcgcacgc
    29281 tgacttccag gcacagcaag gattagccga caagccgctg attgataaag agatcggcga
    29341 agtgctggcg acccatgacg aaacgcaacg tcaggcgctg tatcgcgaca ttttgacccg
    29401 tctgcatgac gaggcggttt atctgcctat cagctacatc tcgatgatgg tggtatcaaa
    29461 accggagctg ggtaatatcc cctacgcgcc gatcgccacc gaaattccgt tcgaacagat
    29521 taaaccggtg aaaccttaat gttgcgttac gtattacgcc gctttctgct gctgatcccg
    29581 atggtgctcg ccgcctcggt gatcattttt ctgatgctgc gcctcggtac cggcgacccg
    29641 gcgctcgatt atctgcgttt atctaacctg ccgccgacgc cggagatgct ggcctctacc
    29701 cgcaccatgc tcgggctgga tcagccgctg tacgtccagt acggtacctg gttgtggaag
    29761 gcgctgcatc ttgactttgg tatctcattc gccagccaac gcccggtgct ggacgatatg
    29821 ctgaacttcc tgcccgccac gctggaactt gcaggtgcgg cgctggtatt aattctgctc
    29881 acttccgtac cgctcggtat ctgggcggcg cgccatcgcg accgtctgcc ggatttcgcc
    29941 gtacgtttca tcgcgtttct tggcgtgtcg atgcctaact tctggctggc gtttttactg
    30001 gtgatggcgt tttcggtgta tctgcaatgg ctacccgcga tgggttacgg cggctggcag
    30061 cacatcattt tgcctgcggt ttccattgcc tttatgtcgc tggcgattaa cgcgcgactg
    30121 ctgcgcgcca gtatgctgga cgtcgccggt cagcgtcacg tcacttgggc gcgtctgcgc
    30181 ggcctgaacg acaaacagac cgaacgccgc catattctgc gcaatgcctc gctgccgatg
    30241 atcaccgccg tggggatgca catcggcgaa ctgattggcg ggacgatgat tatcgaaaac
    30301 atctttgcct ggccgggcgt cgggcgctat gcggtgtcgg cgatttttaa ccgtgactat
    30361 ccggtgattc agtgctttac gctgatgatg gtggtggttt ttgtggtctg taatttgatt
    30421 gtcgatttgc tcaacgccgc gctggacccg cgcattcgtc gtcatgaagg agcgcacgcg
    30481 tgaacttttt cctctcttcc cgctggtcgg tacgcctggc gctgatcatt atcgccctgc
    30541 tggcgctgat tgcgctcacc agccagtggt ggctgccgta tgacccccag gcgattgatt
    30601 tgccgtcgcg cctgctttcg ccggatgcgc agcactggct gggcaccgat cacttaggtc
    30661 gcgatatttt ctcgcggctg atggcagcga cccgcgtgtc gctcggttcg gtaatggcct
    30721 gcctgctgct ggtgctgaca ttagggctgg ttattggcgg cagcgccggg ttgattggcg
    30781 ggcgcgttga tcaggccacc atgcgcgtcg ccgatatgtt tatgaccttc ccgacctcga
    30841 ttctgtcgtt ctttatggtt ggcgtgctcg gcaccgggct gaccaacgta attatcgcca
    30901 tcgccctgtc gcactgggcg tggtatgcac gcatggtgcg cagcttggtg atttcactac
    30961 gccaacgcga gtttgtgctg gcgtcacggc tttccggtgc gggccatgtg cgggtgtttg
    31021 tcgatcatct ggcaggcgcg gtgatccctt cgctgctggt gctggcaacg ctggatatcg
    31081 gccatatgat gctgcacgtc gcggggatgt ctttccttgg cctcggtgtg accgcgccga
    31141 ccgccgaatg gggcgtgatg attaacgacg cgcgccagta tatctggacc cagccgctgc
    31201 aaatgttctg gccggggctg gcgctgttta tcagcgtgat ggcctttaac ctggtgggtg
    31261 acgcactgcg cgatcatctg gatcctcatc tggtgacgga gcacgcacac taatgccgca
    31321 acagattgaa ctacgtaata tcgcgctaca ggccgcgcag ccgctggtac acggtgtatc
    31381 gttaaccctg caacgcgggc gcgtgctggc gttagtcggc ggtagcggca gcgggaaatc
    31441 attaacctgc gccgcgacgc tgggcatttt gcccgctggc gttcgccaga cggcggggga
    31501 aattttagcc gatggcaaag cggtttatcc ctgcgccctg cgcggcatca aaattgccac
    31561 catcatgcag aacccgcgca gcgcctttaa tccgctacac accatgcaca cccacgcgcg
    31621 ggaaacctgc ctggcgttag ggaaacccgc cgatgacgct acgcttaccg ctgccataga
    31681 agcggtgggg ctggaaaacg ccgcgcgcgt gctgaagctg tacccgttcg agatgagcgg
    31741 cggcatgttg cagcgcatga tgattgcgat ggcggtgctg tgtgaatcac cgtttatcat
    31801 cgccgatgaa ccgaccaccg acctcgacgt ggtagcacag gcgcgcatcc tcgatctgct
    31861 ggaaagcatt atgcaaaaac aagcgccggg aatgctgctg gtcacccatg atatgggcgt
    31921 tgtggcgcgt ctggcggatg acgtggcggt gatgtcacaa ggtaaaattg tcgaacaggg
    31981 cgatgtagaa acgctgttta acgcccccaa acatgcggta acgcgcagcc tggtttccgc
    32041 tcatctcgcc ctctacggta tggagctggc atcatgactt tacttaacgt ctccggcctt
    32101 tcccatcact atgcccacgg tggatttaac ggaaaacatc aacatcaggc ggtgctgaat
    32161 aacgtttccc tgaccctgaa aagcggcgaa accgtcgccc tgctggggcg cagcggctgc
    32221 gggaaaagta ccctcgcgcg gttgctggtg ggtttagaat cgcccgcgca ggggaatatt
    32281 agctggcgtg gcgaaccgct ggcgaaactc aatcgcgccc agcgtaaagc gttccgccgc
    32341 gatattcaga tggtatttca ggactccatc agcgccgtga atccgcgcaa aaccgtgcgc
    32401 gagatcctgc gtgaaccgat gcgccacctg ctatcactga aaaaatccga acaactggcg
    32461 cgcgccagcg aaatgctgaa ggcggtcgat ctcgatgaca gcgttctcga caaacgccca
    32521 ccgcagttaa gcggcggcca gctccagcgc gtctgcctgg ctcgcgcgct ggcggtcgaa
    32581 ccgaaactac tgattctgga tgaagccgtt tctaaccttg atctcgtgtt acaggcgggc
    32641 gtcattcgcc tgctgaaaaa gctacaacaa cagtttggca ccgcctgcct gttcatcacc
    32701 cacgacttac gcctggtgga acgcttctgt cagcgggtga tggtgatgga caacggacaa
    32761 atcgtcgaaa cccaggtggt gggagataaa ttaacctttt cctctgacgc cggacgtgtg
    32821 ctacaaaacg cggtattacc cgcattcccc gtgcgccgtc gcaccacaga aaaggtttaa
    32881 cgcaaatgca acgagtcacc atcacgcttg atgacgattt actggagacg ctggacagcc
    32941 tgagccagcg tcgtggttat aacaaccgtt ccgaagctat ccgcgatatt ctgcgtagcg
    33001 ccctggccca ggaggccact cagcagcacg gcacgcaagg tttcgcggtg ctctcctatg
    33061 tgtatgaaca cgaaaaacgc gacttagcca gccgcattgt ctccacccag caccatcacc
    33121 acgatctctc cgtcgccacg ctgcatgtgc atatcaatca cgacgactgt ctggaaatcg
    33181 ccgtgctgaa aggtgatatg ggcgacgtgc agcattttgc cgatgacgtt atcgcccagc
    33241 gcggcgtgcg gcatgggcat ttgcagtgct tgccgaagga agattgagtt atttctgaaa
    33301 cgaggaaaga aaagatatta actgaactta atttataaaa ttaagttcag ttgcatagat
    33361 agatcctgta aaggcagata ttctgcgtaa tgtacgccgc taaaatttat aaatgattca
    33421 aacgctcatt cagtgtttca ataatatagg tattaacaga gacttgttgc tgcgcagccg
    33481 cattattaag gcgttcactc agcgattctg gatagcgtaa cgtaaaggtc ttaatctttt
    33541 ctgtacgcgc atacggctca atacccgcag ctttacaatc ctcaagatac tctcgcaggg
    33601 aaagttcccc ttctttttgc agcccctgaa tgctgtcaga gacaaaatca caataacctg
    33661 acaagcccag gaatttgcca cgaaatgcat tcagttctgg cacgtaggta ataacagctg
    33721 gttgcccggc aatctccata gagtttggcg tcttcagttt gatcatggtt ttacccctat
    33781 acttagtaac cagtcacgca cgctttctac agcgccttta tcggtatctg gcgacggatg
    33841 aggccgatgg aaacacgcaa cgctcatatt tagtatgaac ttacaacgcg aacctcttcc
    33901 ttccttaatt tctccgccta atgctttaac cagtgactct atatcagacc acttgatccc
    33961 ttgaggaaca ggagttttaa atatttgatc caatgtattt ttttttcttc cgtaaagcct
    34021 gtaccttggt tctcatggca tgtccttatg acttcacttt atgacatcaa tagtatgaca
    34081 tcacagtatg aagtcaagtt tcccttcctt acgccattgt cccaatcgtc ttcctgaatc
    34141 gcagcagcgc aatggtgaaa aacgcgccgc caattgccat cagcgtcagg aactgcggcc
    34201 agacgatttc gaatccggca ccccggtaga gaatggcctg cgcgaggcta acaaagtgtg
    34261 tcgtcggcat ggtcagcata atgtcctgca ccatctgcgg catactttcg cgcggcgtgg
    34321 atccaccgga aagcatttgc agcggcagca gcaccagaat caccagcagc cccagttgcg
    34381 gcattgaacg cgctatcgtc cccataaaaa tgccgattga cgtggtggca aacagactga
    34441 gcgccacgcc cagcataaac agcgggatcg agccttcaat cggtacgccc agtacacctt
    34501 tcgccatcag caccagcgat aatcccgata ccaccagcac caccagcccc atcgaccaga
    34561 tcttcgccat catgatctca aacggcgtta tcggcatcac cagtaagtgt tccaccgtgc
    34621 cgtgttcacg ctcgcggatc agcgccgatc cggtcaatac aatcgccagc atggtaatgt
    34681 tgttgatgat cgccatcacc ccgccaaacc acgcgggatc gaggttcggg ttaaagcgca
    34741 tccgggtttc cagcgatacc aacggttcgc tgttatcacg gtagcgcgcg acaaagctgt
    34801 tcacttcacc gttgataata ttctggatat acccattgcc ggtaaatgcc tggctcatgc
    34861 gcgtggcatc gacgttcacc tgaatatccg gctggcgtcc ggcgaggaca tcacgctgaa
    34921 aattaggcgg aatatttatc gcgaaggtat agcgtccggc gtccagtccg gcatccatct
    34981 catcggcggt gatcatctcc ggtggcaaaa accacggacg atagaagctg ttaacgatcc
    35041 ggttcgataa ctgcgattga tccatatcgg caatggcgat cggcgcgagg ttcaacgatc
    35101 ctggcgtaac ggtcgctgac gaatacaccg acaccgtaaa cgagaagaca atcagcgtca
    35161 gcatcgcttt atcaccgagc agactgtgca actctttgat acccagatta aaaatattgc
    35221 gtaaatggcg catcatccct cctgtttttt cagcagcagg atgcttaagc ccatcaccag
    35281 cgggatggct atcagcaacg ggataaaaag ttgccacaaa tcagtcagat ccagcgcttt
    35341 cgagaacgtt ccgcgggcga tagtcagaaa atgactggtc gggtaaacct cgccgatcca
    35401 acgtccaggc ccttccagcg aagctaccgg atcgatcatc ccggaaaact gtgtcgccgg
    35461 gatcaacgtg ataatcgccg ttccgaaaat ggcggcaatc tggcttttca taaaggtgga
    35521 gatcagcagc cccattccgg tggcaatgat gatatacagc agcgccgcca gggtgagcgt
    35581 caggaaactg cctttatgcg gtacgccaaa cacaaacacc gacaggccgc agagcaggaa
    35641 aaagttcagc atccccagcg cgatgtacgg cagctgtttg ccaagcaaaa attcgctacg
    35701 ggtggtgggg gtcacgtaaa ggttgataat cgaccctagc tctttttccc gcaccacgct
    35761 aagggcgctt agcattgacg ggatcatcat cagcagaagc gggatcaccg ccggaacaat
    35821 cgctggcagg ctttttacgt ccgggttata gcgatagcgc gtctcaatgt tcatcagccc
    35881 gctttggctg gcgggtgtcg attgtcggct cgccacatcc tgaagccaac tctggtgcat
    35941 tgcctgcacg taacctttta ccgtttcggc acgactgggc atcgctccgt cgatccagac
    36001 gccgagttcc acaggcgtac cacgcgcgat atcgcgcccg aaattgggcg ggatctcaat
    36061 cgccaccgtg atatcgcccg cacgcatccg acgatcaagc tcgtcataac tggtgagcgg
    36121 cggctgttcg ataaagtaac gggaaccgga gaggttgagt gtccacgcct ggctactgac
    36181 ggtctggtcg cggtcgagca ccgcaaagcg caggttttcc acatccatac tgatgccgta
    36241 acccattatc agcatcagga tcaccgttcc catcagcgcc agcgtcgaac gtactggatc
    36301 gcgtcgcagt tccagcgctt cgcggcggct gtagctaaac agacggcgca ggctaaatcc
    36361 ctgacgcggc gcgtgggtgg tgtcgtgtac cacgggcggc gcttcggctt cgttgctctg
    36421 ccctgccgct tcctgcaaat aggcgataaa tgcctcttcc agactggcgg ctccgcgttt
    36481 ctcaaccagt tcctgcggtg taccgctggc aagcactttt ccggcgtgca tcagtgagat
    36541 gcggtcgcaa cgttccgctt cgttcataaa gtgggtagag atgaagatag tcactttgtc
    36601 ctggcgcgag agatcgacca tcaactgcca gaacatatcc ctcgccaccg gatcgacacc
    36661 agaagtaggc tcatcgagga ttaacatctc cgggcgatga atcaccgcca ccgccagcga
    36721 aagccgctgg cgaatgccga gcggcaatga ctccggcaga acatcttcaa cgtcgttgag
    36781 cttaaaacgc tcgctcattt cagccactct tgcgggaatt tccgcttccg ggatgtgaaa
    36841 caaacgggca tgtaactcaa ggttttgccg cacggtgagt tcgttataga gcgaaaacgc
    36901 ctgcgacata tagcccaccc gacggcgggt atcgatatct tttggatcaa ccggttgccc
    36961 gaacagccac gcctcacctt cgctggcggg cagcagtccg gtgagcattt tcatggtggt
    37021 ggatttaccg cagccgttcg aaccaagaaa accaaaaatc tccccgcgtg gaatgcggaa
    37081 attaacgtga tcaacggcaa cgaaggaacc aaaacgcatg gtcagatcgc gcgcttcgat
    37141 ggcaatctct gcgttttcag gttgatacgg tgggatcact accgcctgat gcgcctggcg
    37201 ttgcgcttgc ggtaacagat ttataaatgc ttcttccagc gtagcgcttt gcgtttgctg
    37261 ccgtagctct tcggcgctgc cagttgccag cacttctccg gcattcatcg ctaccagcca
    37321 gtcgaagcgt tcggcctctt ccatataggc ggtggcgacc agcacgctca tattgctctg
    37381 ccgctggcga atactgtcga tcagatccca gaactgggag cgggagagcg ggtcaacccc
    37441 cgttgttggc tcatcaagga tcaacagttc cgggtcgtgg attaacgcgc agcacagccc
    37501 aagtttttgc ttcatcccgc cggagagttt ccctgccgga cgatcgcgaa acggtgctaa
    37561 ccccgtgctg gtcagcagct cattaattcg cacttcccgc tccgctttgt cgtgaccgaa
    37621 caggcgagcg aaaaaatcga cgttttcata caccgacaag gtgtggtaga ggtttttgcc
    37681 cagcccctgc ggcatccagg cgatgcgcgg gcagacgtcg cggcgatgcc tcgggtcgcg
    37741 catatcgccg cccagcacca tcacattgcc ctgctcaatg acgcgggcac cggaaatcaa
    37801 cgacaataag ctcgacttcc cgacgccatc cggaccaatc agcccgacca tacagcgggc
    37861 cggaatatcg agagtaatat tgttcagcgc aacgtttttt ccataatgct ggctcacgcc
    37921 cgccagttgc gcgacaggcg ggacgggaac cagttccaga tgcgtcattg cggcaacctc
    37981 accacgaggt cgtcaggcca cggaagttct tcattcaccc gcacccacgc tacgcccggt
    38041 aaaccggttt tgacatattc cagatgctgc tggagtaatt ccggtgggat acgcgctttg
    38101 acgcggaaca tcagtttcag ccgttcatcg ctggtttcga cggtttttgg cgtgaactgg
    38161 gcgacactgg cgacaaaact gatggtcgcc ggaatacgca gatctggcgc ggcatcgagg
    38221 atcagccggg cttcaccgcc aagtttcagc gtgcccgcct gttcggttgg caggaagaaa
    38281 gtcatataga cgtcgctgag atcgaccata ttcagcaccc gaccgcctgc cgccagcact
    38341 tcccctggct cagcaacccg atactgcacg cgtccgtcac gcggggcttt cagttcactg
    38401 tcatcgatat ctgcggcaat acgccgttca gtggcttgcg cggcttcgac gcgagtttgg
    38461 gcctgaatga tattggtacg ggccgcttct atagccgctt tagaagccga tacctgagct
    38521 tttgccgatt ccagcgcagc tcgggcgctc tcagcggcgg cgcgatcgtc atccagctgt
    38581 tgcgcagaaa tagcccctcg ttgggcgagc gagcgggaac gcgtatgacg tttagccacg
    38641 gagtccagtt cggactggcg ttgattaacc agcgactgcg cggcacgagt ttcgctttgc
    38701 cgttgctcca gcaaagcctg cgcggcagca acggcacttt gtgcttcttt gatttgcgcg
    38761 atggcttcca gtcgctgctc ctgcaacacg cgagtatcca tcttcgccag cacttcgcct
    38821 tcgcgaacaa actggccttc tttcaccaga atggtgtcga tacgcccggc aattttgctg
    38881 gcgatatcca cttccgtcgc ttcaatgcgc ccattgctga cagcaaagcc ttccggcaca
    38941 cccgccgggc gcaacagcca ccacgccacg acggcagcca ccaccagtaa cccgacaacc
    39001 caccacgcca gatggcgctt actcttatcc ataatcgacc cgccataatc cctgcaagca
    39061 aacgacgcgt gttacaactg ccgccgaacg acgttcagcg gattcttcca tccgggataa
    39121 gaaggaaacg acaaatacga ccagcgcgca gagcattgag cgccaacggg gaaggcttag
    39181 tgaaaacggg tagtaatgtt gtcatcatca gcattcctgg ccgtaaatga aacacgcata
    39241 ctcataagtg tgatctataa aactggctga caaaacggta tccatcctgt tggatagcgt
    39301 ttcacgcctg ttcagttgca ggaacagagc attccttttt acgttttatc aacagtctca
    39361 accagatagt aacattaata ttcatgttgt ctatggttca gagctgtaac attgtgcgcc
    39421 ttctgatcaa aggcataagt ccatttctgt ttttttcatg ctggtaagac ttccagggag
    39481 tggttatctc aggccctgca ccagggttaa tttggcaatt atgcatgctg aacattcttc
    39541 cagcatgttt actgtcacgg gcgttaagac ctaaaactgt ccccgtttca cctatacatt
    39601 ctcttccacc attaccgtta tgtgcaacaa cttgatttat acctaacatt tgtgagcatg
    39661 ctaatagtgc ttcaagttta ggtctgtaat ctgtcgaaac agaaaaataa cgattaaagg
    39721 atcgacaaga gatagcccgg ctatttttaa actttttgcc attaattaca gcatggtgct
    39781 ttttattgat aattgccgct aattcgaccg gatcgagagc attggtcatt tcatcactag
    39841 agactgttat cggtcctata atgtaacgtt ttcgctgatc atcaaaagtt atcccatgat
    39901 gatttgccat tattttgtat ttggggtcat agaaacatac aggataatct tttatcccag
    39961 agtaggtatc ctcatcaagt ggtttcatgc gcgtgaagtt ctgcatatag ttaccattgc
    40021 aattcgtttc atgatttcca gctaatacat gaacgttttc attgatactc attatgcgtt
    40081 ttaataaatc gatgataaat ttatcgccac agatattact aaatcgatcg ccagtctggt
    40141 cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aaccaaataa ggctgaagac
    40201 tttctaaaac aatgtctctt ctgttcagga tactgattat tatattaaca ttagtgtcaa
    40261 gttgctcatt aaagttctcc cttagcaata cgttgagaac ttcatcttct atattaagca
    40321 actggctaag gaaattttct ccttcagcca tgattcccaa atgtcccgtt gcaaaaagcg
    40381 cgtacaaaac tgcaccgact gagccatcgg tatcgccaaa ataggtcggt ctggtacttg
    40441 tgggatcaac aggtgaacaa ttggagaaat aggttctgtc agcgaactgg ggttttatca
    40501 ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc cagatgggtt
    40561 tgtacgactc agtaactgca acatcattag cattatcatt tattgtcata ttattaagta
    40621 aataaacaat gctgcaccaa ttaatatcgg tattatcaag agggcaaccg taatttttac
    40681 tattaattgt cacaataaac tgataacagt tattcctacg tagaatatga gcgcgcatgc
    40741 ccgatagcat actatatttt tctgaatgat tcgtttgtat ggtaaatgaa attttgtcat
    40801 cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc acaacctcac
    40861 cgagcattgt cctggcaata cttgatatat catcacctat accatttgca atatacccaa
    40921 acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt gtgattgtcg
    40981 ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc atattattta
    41041 ccgcgaggaa tacatagtta acatttaatc ttatgacata cacctcatgt tcgatgggaa
    41101 aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat ttcttatctc
    41161 atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgcct ctacaatcga
    41221 ttaagaaaac ctatgaaata ctggcaaaat taaaaagaga taccacagca ctcatgaagt
    41281 tttattgagg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca cgttcgttag
    41341 gtgctatctt ccaggaattt agccccatgg tataattatc aattttttga tgctgatcta
    41401 cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt ccacatttga
    41461 agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattt tcatatacag
    41521 gtgtggatgc tacagcattg cttctaatta gatggataag acgatcatcc atgtgttgtt
    41581 tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct ttcatctgat
    41641 taaatggaac ctgcaaagca ccgagacaat aacattcatt ttcttcattt cttattattc
    41701 cgtgatgact ggttaaaact ttcgtttctg agtcataatg acaaacatcg agcgttttaa
    41761 ttaagttata cgtatcaccg gcagagagtt tataattttc tagtcgtgca gtatagttac
    41821 cattaaaatt gatttcatga tttccagcca gaacgatgac atttttatta atacggctat
    41881 ctttattacc atccatattt cgcatagaat ttagcaaagt aaggatatat ttatcaccaa
    41941 aaatagtact aaatctatcg cctgtatgat ccccaaggaa gatacaaggg gttatcgcat
    42001 cgtcctttaa aaaaacatgt tgatgcaact cattcaatat catgtctctc gtatttaata
    42061 aattgtagag caatttatta tttttatgta tgtatgcaga tgaagccatg ttttcgtgtt
    42121 ttaataattc acataataaa ctccaaccct tttccctgat gcctatatgc cctgaaacga
    42181 gcaatgcata aagtacagcg cctactcttc catctgtatc gccaaaataa gccgtgttgg
    42241 cattttgtgg gttaatcaca ccacattgtt tataaaagtc tattttagaa aacacagatt
    42301 taacatagct atcaaactta tccttaggaa acgtctcttt agagacatcc caacgccagt
    42361 cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata gaaagcccat
    42421 taactttata agttatgtct gatttataaa ttatgtctga tttatctccg gagaaattaa
    42481 taaaataata accatcctta cactcaatct taactgtaat gggagacgat aattttatca
    42541 catggttatt gtctggtata attgtaaaga taaacgtgtt agtagtatta tcggcgaaac
    42601 tattatattc ttgaatatct atagatggct cactattgta aaaccatatt ttctccatct
    42661 gaagactatg ttcatttacc ctccagtgac catgttgcag gaaatagatg atattcaggc
    42721 ctaaatcgtt atctatttgc gaaaagtcat tcagcatatt aacttcaact gtgtttgatc
    42781 gatatttttt atcaaccttg ccatggtgtt gatgactgtt aactttatct gaatgcttat
    42841 cttgatggtt tgaagagaag tgcttaaata tattgtgaaa tactttcata aataacctgt
    42901 aatattttta cttgttagta acaaaataaa caatacatgt ctgaaagaca tatttacgat
    42961 gatacaaagc aataatactt atatgttcga tgattttaag caccgagact atccggtctt
    43021 ttactaaata atggaaaaca atccatccct ggaagataat tgtaaaaaaa aacaacagaa
    43081 taagaattaa tcttaggata aatatttatt tatcatggcc ttttaaccgg taaaaaatgg
    43141 cactcggcac agtaaacccc gctatagtta tgctatgcat ttgtctttaa tgatatctcg
    43201 tcgttatatt aacaggctga aattatcatt aatgcacatt tcattattag cagggatacc
    43261 tgaggagttt aaagtgaaca tatatatcgg gtggcttttc aaattaatcc ctttgattat
    43321 gggattaatt tgcatcgcgt taggtggctt tgtgctggaa agttcagggc aaagcgagta
    43381 tttcgtcgcg ggtcatgtgc tgatttctct ggcggccata tgcctggcat tattcactac
    43441 cgcatttatt atcatttcgc agctcacgcg cggcgttaat acgttttaca atacattgtt
    43501 ccccattatt ggctatgcgg ggtcaattat caccatgata tggggttggg cactgttagc
    43561 aggcaatgat gtgatggcag acgagtttgt cgccggccat gttattttcg gcgttggtat
    43621 gattgccgcc tgtgtatcga cggtggcagc gtcatccggt cactttctgc tcattcccaa
    43681 aaatgcagcg gggagcaaga gcgatggaac gccggtacag gcttattctt cattaatcgg
    43741 taattgcctc attgccgttc ccgttttact caccctgctc ggtttcattt ggtctattac
    43801 gctgttacgt agtgctgaca taactccgca ttatgtcgcg ggtcacgtat tgcttgggtt
    43861 aaccgcaatc tgtgcctgtc taattggcct tgttgccaca attgtccatc aaacacgtaa
    43921 tacgttttca actaaagaac actggctgtg gtgttattgg gttatttttc tcggctcaat
    43981 cacggtactg caggggatat acgtcttagt cagttccgat gcaagcgccc gactggctcc
    44041 cggcattatt cttatttgcc tcggaatgat ctgttacagc atattctcaa aagtctggct
    44101 actggcactg gtctggagac gtacctgttc gttagccaac agaataccga tgatacccgt
    44161 cttcacctgc ctgttttgcc ttttcctggc atcatttctt gcggaaatgg cgcagaccga
    44221 catgggatat tttattcctt cgcgagttct ggtcggtttg ggagcggtat gctttacgtt
    44281 gttctcaatc gtttcaatat tagaagcggg ttctgctaaa aaataattgc aacgtaccgg
    44341 ataaaaccag cgttgaccat ttgcgtaacg ctggtttttc ttaggcatca tgaaataacg
    44401 cagcattaat gcatagtggt taaggataaa aaaacaacaa agttactgtc tttttcagcc
    44461 tgttcatatt ttataaagat ctggccactg ggtgagtttt caatcacctt tccttccacc
    44521 ttatattaag cctggagggt ttcagttcac gggctcatta gaaaataatc acaagatagc
    44581 cactatcaaa attagctcat ttttaatgcg gctctaataa ttttcatctt taggtaacag
    44641 gtcaggacga cttcgctgca atcaaatcct gcgcacaagc ccacgcactc gaccacgccc
    44701 actggaagtt atagcccccc agccagccgg taacgtccat cacttcgccg atgaagtaca
    44761 gcccaggcac tttgcgcgct tccatcgtcc gtgaagagag ttcgttggtg tccacgccgc
    44821 cgagcgtcac ttcggcagtg cgatagcctt cagtgccgtt gggttgtacg cgccagtcgg
    44881 tcaatgtgct aatcagtgcc tgttggtcac gcacgttgag ctgttttagc gaaacattcg
    44941 ggatttgccc gagttgctgt aagcgttcaa ccaaccgctt cggtagatga accgccagtg
    45001 tgtttttcag gctctgattc ggatgtgcgt tacgctgctc attcaggaag gtttcgagat
    45061 ccacatccgg tagcagattg atactgacaa attcacccgg ttgccagtag ctggaaatct
    45121 gcaacaccgc cggtccagac aagccgcgat gggtgaagag taagttctca cggaaaacga
    45181 tgccgttttc agcggtaatc acggaaggca ccgccacgcc cgccagcacc tgtaactctt
    45241 cgagcaacgg tttatgcaga gtgaatggca ccagacccgc acgggtcggc agcacgttga
    45301 ggccaaattg ttcggcaatc ttataaccaa acggcgacgc gcccagcccc ggcattgaca
    45361 gcccaccggt cgccatgacc agcttttcgc aaccgacagt catgccgttc agatcaagcg
    45421 tgaagcctgt ttcatcctcc gccacactca gcacttcgct acgcaatctg aaggtcacat
    45481 tgcccttctc gcactcatcc accagcatgt cgacaatctg ctgcgcggag tcatcgcaga
    45541 agagttgccc taacgttttc tcgtgccatg cgatgccgtg tttattgacc agatcaatga
    45601 aatcccactg ggtaaaacgt gcgagtgcag acttacaaaa atgcggattc tggctcagat
    45661 aagcgcctgg ttcgacataa aggttggtaa agttgcagcg cccaccgcca gacataagga
    45721 ttttgcgccc tggtttttta ccattatcga tcagcagaac ccggcgtcct gcctgacctg
    45781 ccagcgcaga acagaacata cccgccgcac cagcgcctat aataatggca tcaaaccttt
    45841 ccacgttgcg ctcctcttag aaaaaacggg cgtgaattgt aaagattcct cagtggtcgc
    45901 accagcatca atattactaa aaggaagtat ttgcctgaat tatataagat aattattttt
    45961 tgagtgaaat ccatacagga ggcaaatcaa aaaaagtcta tatttcactt tgcccgcgcc
    46021 gcgaaagtca ctgataatgc gccgcgttca tgtcctcaaa atggcgtaac gtcctatgct
    46081 acatttgttt gctggcctgg atttgcatac cgggctgtta ttattgcttg cactggcttt
    46141 tgtgctgttc tacgaagcca tcaatggttt ccatgacaca gccaacgccg tggcaaccgt
    46201 tatctatacc cgcgcgatgc gttctcagct cgccgtggtt atggcggcgg tgttcaactt
    46261 tttgggtgtt ttgctgggtg gtctgagtgt tgcctatgcc attgtgcata tgctgccgac
    46321 ggatctgctg cttaatatgg gatcgtctca tggccttgcc atggtgttct ctatgttgct
    46381 ggcggcgatt atctggaacc tgggtacctg gtactttggt ttacctgcat ccagctctca
    46441 tacgctgatt ggcgcgatca tcgggattgg tttaaccaat gcgttgatga ccgggacgtc
    46501 agtggtggat gcactcaata tcccgaaagt attaagtatt ttcggttctc tgatcgtttc
    46561 ccctattgtc ggcctggtgt ttgctggcgg tctgattttc ttgctgcgtc gctactggag
    46621 cggcaccaag aaacgcgccc gtatccacct gaccccagcg gagcgtgaaa agaaagacgg
    46681 caagaaaaag ccgccgttct ggacacgtat cgcgctgatc ctttccgcta tcggcgtggc
    46741 gttttcgcac ggcgcgaacg atggtcagaa aggcattggt ctggttatgt tggtattgat
    46801 tggcgtcgcg ccagcaggct tcgtggtgaa catgaatgcc actggctacg aaatcacccg
    46861 tacccgtgat gccatcaaca acgtcgaagc ttactttgag cagcatcctg cgctgctcaa
    46921 acaggctacc ggtgctgatc agttagtacc ggctccggaa gctggcgcaa cgcaacctgc
    46981 ggagttccac tgccatccgt cgaataccat taacgcgctc aaccgcctga aaggtatgtt
    47041 gaccaccgat gtggaaagct acgacaagct gtcgcttgat caacgtagcc agatgcgccg
    47101 cattatgctg tgcgtttctg acactatcga caaagtggtg aagatgcctg gcgtgagtgc
    47161 tgacgatcag cgcctgttga agaaactgaa gtccgacatg cttagcacca tcgagtatgc
    47221 accggtgtgg atcatcatgg cggtcgcgct ggcgttaggt atcggtacga tgattggctg
    47281 gcgccgtgtg gcaacgacta tcggtgagaa aatcggtaag aaaggcatga cctacgctca
    47341 ggggatgtct gcccagatga cggcggcagt gtctatcggc ctggcgagtt ataccgggat
    47401 gccggtttcc actactcacg tactctcctc ttctgtcgcg gggacgatgg tggtagatgg
    47461 cggcggctta cagcgtaaaa ccgtaaccag tattctgatg gcctgggtgt ttacccttcc
    47521 ggctgcggta ctgctttccg gcgggctgta ctggctctcc ttgcagttcc tgtaatcgta
    47581 cgcaccaaaa cgagcgggtc agctggcccg cttcagattg tgacatagtg cgctttgttc
    47641 atgccggatg cggcgtgaac gccttatccg gcctacaaaa acatgcaaat tcaatagatt
    47701 gcagagatta tgtaggcctg ataagcgtag cgcatcaggc aattttgcgt ttgtaatcag
    47761 tcttgagcgg gtcagtttac tggcccgctt tttttatgcg ttattattgc tgaagtgctt
    47821 aatgccaaat catcaatgca atcaggctga ccaccaccag accacacaat gcgctggtca
    47881 gaataaactg ccgacgcacc cgctcacagc ggcgaataaa ttcatcatcg tgatgatcgc
    47941 gataacgttg ggcatagata taccaaacga gacgcacctg tttgttgggt tggccatgtg
    48001 aggtaaaaaa gccccctcca tcaacatatt gatagagcaa tggatcgcag ttacgcagta
    48061 ccactaacaa cgcgcgtagt gatgagaaat agcgcgccat gttaacaatg caaacgacac
    48121 ataaagccca aaataatgcg acggtgctta tcatacctcc tccccggcga cctgcccgcg
    48181 gagttccacc ccggggctac cgctcccgat acgctgccaa tcagttaaca ccaggtcctg
    48241 gagaaaccgc ttttgtggtg accaacatcc gagcggctct atagatagtg taggagatca
    48301 ggttgttttt tttccagaag gttaaccact atcaatatat tcatgtcgaa aatttgttta
    48361 tctaacgggt aagcaaggcg gattgacgga tcatccgggt cgctataagg taaggatggt
    48421 cttaacactg aatctttacg gctgggttag ccccgcgcac gtagttcgca ggacgcgggt
    48481 gacgtaacgg cacaagaaac gctagctggc cagtcatcga caactttatg gaaggagtaa
    48541 cactatggct tataaacaca ttctcatcgc ggtcgatctc tccccggaaa gcaaagttct
    48601 ggtagagaaa gcagtctcta tggctcgccc ctacaatgcg aaagtttctc tgatccacgt
    48661 agatgtaaac tactctgacc tatacaccgg gcttattgat gtgaatctgg gtgatatgca
    48721 gaaacgcatc tctgaagaga cacatcatgc gctgaccgag ctttccacta atgcaggcta
    48781 cccaatcact gaaaccctga gcggcagcgg cgacctgggc caggttctgg tcgatgcaat
    48841 caagaaatac gatatggatc tggtggtttg tggtcaccac caggacttct ggagcaaact
    48901 aatgtcttcc gcacgtcagc tgatcaacac cgttcacgtt gatatgctga ttgttccgct
    48961 gcgcgacgaa gaagaataat catcccccca cgacgtgttc ctgaacgccc gcatatgcgg
    49021 gcgttttgct ttttggcgcg ccttgttacc tgatgcagcg taaacccctt atctggccta
    49081 cgatctgcgt acgcaatcaa aatctcctgc caatacaaca tttaacacta tcatattttc
    49141 catcattagt gtgatcagct ggttattttc tgttgtaata gtgtattaat ctattcaccg
    49201 catcaatatt aagaatctct gacagatgta aactttttcg cgcgttatcc cttacgcgtt
    49261 catacttttc aggatggtat tggaaggtta ataaatatga atacaacaac acccatgggg
    49321 atgctgcagc aacctcgccc atttttcatg atcttttttg tcgagttatg ggagcgattc
    49381 ggctactacg gcgtgcaggg cgtactggcg gttttcttcg ttaaacagct tggattctcg
    49441 caagagcagg cttttgtcac ttttggtgct tttgctgcgc tggtctatgg cctcatttcc
    49501 attggcggct atgtcggcga ccacctgctg gggaccaaac gcaccattgt tctcggagca
    49561 cttgtgctgg cgattggcta cttcatgacc ggcatgtcgc tacttaagcc tgacctgatt
    49621 ttcatcgccc tggggactat cgctgtcggt aacggcctgt ttaaagctaa cccagccagc
    49681 ttgctttcga agtgctatcc gccgaaagat ccgcggcttg atggcgcatt caccctgttc
    49741 tatatgtcga tcaatatcgg ctcgttgata gcgttatcgc tggcccctgt gatcgctgat
    49801 agatacggtt attcagtcac ctacaacctg tgcggggcgg ggttaattat cgcattactg
    49861 gtttacatcg cctgtcgtgg aatggtgaaa gacattggtt ctgaacccga cttcaagccg
    49921 atgagcttca gcaaactgtt gtacgtatta cttggcagcg tggtgatgat cttcgtatgt
    49981 gcatggctga tgcacaacgt agaagtcgcc aatctggtgc tgattgttct ctccatcgtc
    50041 gtcaccatca tcttctttcg tcaggcattc aagctggata aaaccgggcg caataaaatg
    50101 tttgtcgcct ttgtcctgat gctcgaagcg gtggtgtttt acattctcta cgcccagatg
    50161 ccaacgtcgc tgaacttctt tgccatcaac aacgtgcatc atgaaattct cggcttttcc
    50221 ataaacccgg tcagcttcca ggcgcttaac ccgttctggg tggtactcgc cagcccaata
    50281 ctggcaggca tttacacgca tctgggtaac aaaggcaaag acctctcgat gccgatgaaa
    50341 tttactctcg gcatgtttat gtgctcactg ggctttttga cggcggcagc tgcgggaatg
    50401 tggtttgcgg atgcacaagg gctgacatcg ccatggttta tcgtgctggt gtatttattc
    50461 cagagcctgg gtgaattgtt tattagcgcc cttggcctgg cgatgattgc tgccctggtg
    50521 ccgcagcatt tgatgggctt tattctcggg atgtggttcc tgacgcaggc tgccgcattc
    50581 ttgctgggcg gctatgtggc aacatttacc gcagtgccgg acaacattac cgatccgctt
    50641 gagacgttac ccgtctatac caacgtgttt ggtaagattg gcctggttac gctgggcgtt
    50701 gcagtagtga tgctgttgat ggtgccgtgg ctgaaacgca tgattgcgac gccggaaagc
    50761 cattaattat tcttgcagaa agcagggtag cgttatcgct accctgcttt acttttacac
    50821 gggcgtacct gcatagatat caaagcgatg ccctttagtc acaaccgcgt ttggcgtggc
    50881 gacattcgct agcggtggcg cgtagtccgg gcgctttact accacgcgtt tggtcgccag
    50941 caagcgtgca ggctccagca atccatcggc atcaagatcc ggccccacca gcgactgaaa
    51001 gacacgcatc tcttttttca ccagcgcgct tttctgctta tgcgggaaca tcgggtcgag
    51061 atagaccacc tgcgggcgtg gggtaatatc ggttagcgcc gtcaggctgg aggcgtgaat
    51121 taactgcaaa cgctcctgca accagccgcc gatttccgca tccgcataac cacgcgccag
    51181 gccgtcgtcg agcagcgcgg caacaactgg attacgctcc agcatccgca cgcggcaacc
    51241 gactgaagcc agcacaaagg catcgcgccc cagtcctgca gtggcatcca ccacatccgg
    51301 caaataatcg cctttaatgc ccaccgcttt cgccaccgcc tcaccgcgac caccgccgaa
    51361 tttgcgtcgg tgcgccatcg ctccgccaac aaaatcaaca aagatgccgc caagttttgg
    51421 ctcatcacgc ttgcgcaatt ccagatgttc cggcgttaac accagcgcca tcaggttgtc
    51481 ttcatcgtgc tccagccccc agcgggccgc cagaacagat aaggcgccgt ctccggcgcc
    51541 tgtttcatca attaagcaga ttttcactga atgatcagcc cttaatgccg taatgctcca
    51601 gcatcgcatc cagctgcggt tcacgaccac ggaagcgttt gaacagatcc atcggctctt
    51661 ctgaaccgcc acggctcaga atgttgtcga ggaacgactg cccggtttca cggttgaaaa
    51721 tgccctcttc ctcaaagcgc gagaaagcat ctgccgccag tacgtcagcc cacaggtagc
    51781 tgtagtaacc tgccgcataa ccaccggcga aaatatggct gaaagcgtgc gggaaacggc
    51841 cccaggacgg agatggcacc acggcaacca gtttcttgat ttctgccaga gtttcgagga
    51901 tttttgcccc ctgatccggg cggaactcgg catgaaggcg gaaatcaaac aggccgaact
    51961 ccagctgacg cagaataaac agcgccgcct ggtagttctt cgccgccagc attttatcca
    52021 gcaactcttt cggcagcggt tcgccggttt catagtgacc agagataaac gccagcgcct
    52081 ccggctccca gcaccagttt tccataaact gactcggcag ttcgaccgca tcccacggca
    52141 caccgctgat accggaaaca ccagcggttt cgatgcgggt cagcatatgg tgcaggccgt
    52201 gaccgaactc gtggaacagg gtgatcactt cgtcgtgagt gaacagcgcc ggtttaccat
    52261 ttaccgggcg gttgaagtta caagtcaaat acgcgaccgg tttttgcaga gaaccatcag
    52321 ctttacgcat ctggcctacg cagtcatcca tccacgcccc gccgcgcttg ttttcacggg
    52381 catacagatc gaggtagaag ctgccgcgca gttcgttatt ttcgtcatac agttcgaaga
    52441 aacgtacatc cggatgccag acatcaacct ctttacgctc tttagcggtg atgccgtaaa
    52501 tacgtttcac cacttcaaac aggccgttaa ccgctttgtt ttccgggaag tacggacgca
    52561 gctgctcgtc gctgatgctg tagaggtgct gtttctgttt ttcgctgtag taagcgatat
    52621 cccacggctg caactcatcg acaccaaatt cggctttagc gaaggcacgc agttgctcca
    52681 gctctttttc gccttgcgga cgcgcgcgtt ttgccagatc ggttaagaaa tccagcacct
    52741 gctgcgggtt ttctgccatt ttagtggcaa gggatttaaa ggcgtagttt tcaaagccca
    52801 gcagttgcgc cagttcgtga cgcagcgcga ggatctcttc catcaccttg ctgttatccc
    52861 acttaccggc gttcgggcct tgatcggagg cgcgggtgct gtaagcgcga tacatctctt
    52921 cgcgcagagc ctggttgtcg cagtaggtca ttaccggcag gtagcttggg atatccagcg
    52981 tcagcaggta gccttccagc tctttcgctt cggcctgggc ttttgccgca gccagcgcgc
    53041 tttctggcat ccccgccagc cccgcttcgt cggtaacgag tttggtccag cccatcgtcg
    53101 catcgaggac gttgttgctg tactggttgc ccagttcaga aagacgggta gcaatttcgc
    53161 cgtaacgctg ctgtttctct ttcggcagac ctatgccaga gagttcaaaa tcacgcaggg
    53221 cgttatcaac cgctttcttc tgcgccgtgt tcagcgtggc gtaatgatcg ccatcgcgca
    53281 ggtcgcggta cgctttatac agcccttcat gttgccctac ccaggtgctg tattccgaca
    53341 gcagcggcag ggtttgctcg taggcttcac gcagttccgg gctatttttc accgagttca
    53401 ggtggctgac cggggaaaag atacgcccca acacatcgtc cacttccgcc aacggctggc
    53461 agagattttc ccaggtgtac ggtgcccctt gcgctactac gcgcgccaca ttttcgcggc
    53521 aatcgttcag cgcctgagtc acggctggaa cgacatgttc cgggagaatt ttagaaaacg
    53581 gaggcaattc aaagggagtc agtaacggat tcgtcattcg cgcagtcctg gttaaagagg
    53641 ttaaggaagc gctcaacagg cgctttacat aatgtgtgta gcatggggtt aagtgtagtg
    53701 aatttcaatg agaaacgtta cgctttcgcg gcggcggcct cttttcggta tactgtcctg
    53761 atacgctttt gtgcgccccg aatacgggcc gatttttact taccggaaca cctttaccca
    53821 tgctcagtta tcgccacagc tttcacgctg gcaaccacgc cgacgtcctt aaacataccg
    53881 ttcagagcct gatcatcgag tcgctgaaag agaaagataa accgtttctc tatctcgata
    53941 cccacgcagg ggccgggcgt tatcagttag gcagcgaaca tgccgagcgt accggcgaat
    54001 atctcgaagg tatcgcccgt atctggcagc aggacgattt gcccgcagaa ctggaggcgt
    54061 acatcaatgt ggtaaaacac ttcaaccgta gcggtcagtt gcgttactac cccgggtcgc
    54121 cgttgattgc tcgccagcta ctgcgcgaac aagacagcct gcaactgacc gaactgcacc
    54181 cgagcgatta cccgttgctg cgttctgaat ttcagaaaga cagccgtgcg cgcgtcgaaa
    54241 aagccgacgg tttccagcag cttaaggcca aacttccgcc ggtttcccgc cgtggtttaa
    54301 tccttatcga cccgccgtat gaaatgaaaa ccgactatca ggcggtggtc agcgggatag
    54361 cagaaggtta caaacgtttc gccactggta cttacgcact gtggtatccg gtggtgctgc
    54421 gtcagcaaat taagcgcatg atccacgatc tggaagcgac cggtattcgc aaaattctgc
    54481 aaattgaact ggcggtactg ccagacagcg atcgccgtgg catgaccgct tccggcatga
    54541 ttgtgattaa cccgccgtgg aaactggaac agcagatgaa taacgtgctg ccgtggctgc
    54601 acagcaaact ggttccggca ggcaccgggc acgccaccgt aagctggatc gtgccggagt
    54661 aattgcagcc attgctggca cctattacgt ctcgcgctac aatcgcggta atcaacgata
    54721 aggacactct gtcatgacta aacactatga ttacatcgcc atcggcggcg gcagcggcgg
    54781 tatcgcctcc atcaaccgcg cggctatgta cggccagaaa tgcgcactga ttgaagccaa
    54841 agagctgggc ggcacctgcg tgaacgtcgg ctgcgtgccg aaaaaagtga tgtggcacgc
    54901 ggcgcaaatc cgtgaagcga tccatatgta cggccctgat tatggtttcg ataccaccat
    54961 caacaagttc aactgggaaa cgttgatcgc cagccgtacc gcctatatcg accgtattca
    55021 tacttcctat gaaaacgtgc tcggtaaaaa taacgttgat gtaatcaaag gctttgcccg
    55081 cttcgttgat gccaaaacgc tggaggtaaa cggcgaaacc atcacggccg atcatattct
    55141 gatcgccaca ggcggtcgtc cgagccaccc ggatattccg ggcgtggaat acggtattga
    55201 ttctgatggc ttcttcgccc ttcctgcttt gccagagcgc gtggcggttg ttggcgcggg
    55261 ttacatcgcc gttgagctgg cgggcgtgat taacggcctc ggcgcgaaaa cgcatctgtt
    55321 tgtgcgtaaa catgcgccgc tgcgcagctt cgacccgatg atttccgaaa cgctggtcga
    55381 agtgatgaac gccgaaggcc cgcagctgca caccaacgcc atcccgaaag cggtagtgaa
    55441 aaatgccgat ggtagcctga cgctggagct ggaagatggt cgcagtgaaa cggtggattg
    55501 cctgatttgg gcgattggtc gcgagcctgc caatgacaac atcaacctag aagccgctgg
    55561 cgttaaaact aacgaaaaag gctatatcgt cgtcgataaa tatcaaaaca ccaatattga
    55621 aggtatttac gcggtgggcg ataacacggg tgcagtggaa ctgacaccgg tagcagttgc
    55681 agcgggtcgc cgtctctctg aacgcctgtt taataacaag ccggatgagc atctggatta
    55741 cagcaacatt ccgaccgtgg tcttcagcca tccgccgatt ggtactgttg gtttaacgga
    55801 accgcaggcg cgcgagcagt acggcgacga tcaggtgaaa gtgtataaat cctctttcac
    55861 cgcgatgtat accgccgtca ccactcaccg ccagccgtgc cgcatgaagc tggtgtgcgt
    55921 tgggccagaa gagaagattg tcggtattca cggcattggc tttggtatgg acgaaatgtt
    55981 gcagggcttc gcggtggcgc tgaagatggg cgcaaccaaa aaagacttcg acaataccgt
    56041 cgccattcac ccaacggcgg cagaagagtt cgtgaccatg cgctaaatgt taaagggcta
    56101 agagtagtgt gctcttagcc cttaattacg tttccgctat cagttcagaa gctgaagcag
    56161 aaagcggatc agttccagca gcgcaattaa cgctccaaga acgatgattg ctttatcaat
    56221 cacccgtttt ctccatgcga tggagtgaga atgcatccgc ttactcatcc actgcctgtc
    56281 acggcgcatg tctcattgtt agatgacgac tatctcactc cggccagagc atcagttaac
    56341 ggcaccaccc gtacttctga ccaggacttt gaaagcgttt atgcgcatta ccagagtgaa
    56401 aatgcctcag agctaactgg ataatcatac agtacattca ggttataaaa ccagcatgtc
    56461 cttgcaatag tttcagtatg gtattagcat tgatgcgtta gatgatgact atctcactcc
    56521 agccagagcc accaactcag ggctgaagag taaaaaaacc gacgcaaagt cggttttttt
    56581 acatccggat tcggacaagg cttaatatga cgatgaccca gtgaaagtat ataaatcgtc
    56641 attgcgatat ataccgaagt gccccctccg ccagctgaag aaatcgctaa ttcttgcaat
    56701 gttagccact ggctaatagt attgaaccgt tagatgtggg cattctcact ccagccagag
    56761 ccaccaactc aggctgaaga gtaaaaaacc gacgcaaagt cggttttttt acgtcctgat
    56821 tcaaaccgcc tttcaaatga atagccaact caaaattcac acctattacc ttcctctgca
    56881 cttacacatt cgttaagtca tatatgtttt tgacttatcc gcttcgaaga gagacactac
    56941 ctgccacaat caggagcgca atatgtcatt tctgttaccc atccaattgt tcaaaattct
    57001 tgctgatgaa acccgtctgg gcatcgtttt actgctcagc gagctgggag agttatgcgt
    57061 ctgcgatctt tgtactgccc tcgatcagtc gcagcccaag atctcccgcc acctggcatt
    57121 gcttcgtgaa agcgggctat tgctggaccg caagcaaggt aagtgggttc attaccgctt
    57181 atcaccgcat attccagcat gggcggcgaa aattattgat gaggcctggc gatgtgaaca
    57241 ggaaaaggtt caggcgattg tccgcaacct ggctcgacaa aactgttccg gggacagtaa
    57301 gaacatttgc agttaaaaat ttagctaaac acatatgaat tttcagatgt gttttatccg
    57361 ggaggcatta tgttactggc aggcgctatc tttgtcctga ccatcgtatt ggttatctgg
    57421 cagccgaaag gtttaggcat cggctggagt gcaacgctcg gcgcagtact ggcgttagtt
    57481 acgggcgtgg ttcatccggg tgatattccg gtggtgtgga atatcgtctg gaacgcgacg
    57541 gctgcgttta tcgccgtcat tatcatcagc ctgctgctgg atgagtccgg cttttttgaa
    57601 tgggcggcgc tgcacgtctc acgctggggt aatggtcgtg gtcgcttgct gtttacctgg
    57661 attgtcctgc tcggtgctgc cgttgccgcc ctgtttgcca atgatggcgc ggcgcttatt
    57721 ttgacgccga ttgtcatcgc catgctgctg gctttaggat tcagtaaagg cacgacgctg
    57781 gcgttcgtga tggcggccgg attcattgcc gataccgcca gcctgccgct tattgtctcc
    57841 aacctggtga atatcgtttc cgctgatttc tttggcctcg gctttcgcga atacgcctcg
    57901 gtgatggtgc cggtggatat cgccgcgatt gttgccacgc tggtgatgct acatctctat
    57961 tttcgcaaag atattccgca gaactacgat atggcgctgc tgaaatctcc cgcagaagcg
    58021 atcaaagatc ctgctacgtt caaaactggc tgggttgttt tactgcttct gctggtggga
    58081 tttttcgtcc tggaaccgct cggcattccg gtgagcgcca ttgcagctgt gggcgcgctg
    58141 atattatttg tcgtcgctaa acgcggtcat gcgattaata cgggtaaagt cctgcgcggt
    58201 gccccctggc agattgtcat cttctcgctc ggcatgtatc tggtggttta tggcctgcgc
    58261 aatgccggat taacggaata tctttctggc gtactcaacg tgctggcgga taacggcctg
    58321 tgggccgcga cgctcggcac cggattcctc accgccttcc tctcttctat tatgaacaat
    58381 atgccgacgg tactggttgg cgcgttgtcc attgatggca gcacggcatc tggcgttatc
    58441 aaagaagcga tggtttatgc caatgtgatt ggctgcgatt tgggaccgaa aattacccca
    58501 attggtagcc tggctacgct actctggctg cacgtacttt cgcagaagaa tatgactatc
    58561 agctggggat attacttccg tacagggatt atcatgaccc tgcctgtgct gtttgtgacg
    58621 ctggctgcgc tggcgctacg tctctctttc actttgtaat gagatactga tatgagcaac
    58681 attaccattt atcacaaccc ggcctgcggc acgtcgcgta atacgctgga gatgatccgc
    58741 aacagcggca cagaaccgac cattatccat tatctggaaa ctccgccaac gcgcgatgaa
    58801 ctggtcaaac tcattgccga tatggggatt tccgtacgcg cgctgctgcg taaaaacgtc
    58861 gaaccgtatg aggagctggg ccttgcggaa gataaattta ctgacgatcg gttaatcgac
    58921 tttatgcttc agcacccgat tctgattaat cgcccgattg tggtgacgcc gctgggaact
    58981 cgcctgtgcc gcccttcaga agtggtgctg gaaattctgc cagatgcgca aaaaggcgca
    59041 ttctccaagg aagatggcga gaaagtggtt gatgaagcgg gtaagcgcct gaaataaagc
    59101 ggcgatatcc actgccgata gttggtaaag tgcgctttgt ttatgtcgga tgcgacgcta
    59161 acgcgtctta tccgacctac aaaaccgtgc aaattcaata tatggcatga aatatgtaga
    59221 gcgtagcgca tcaggcaatt ttgctattta cgcagcactc ctcgcgtaag aattcaatta
    59281 tcgcttccag cattgcgtat tgcgaaacac acatcagcgt ccgactctca cggttttgtt
    59341 tgaccagtcc taccgatacc agtgctgata tatggtggct gagtgtggag ccaggtatcc
    59401 caagctgctt ttgcaactca ccgaccggca acccttgttc cccggctttg acaagatgtt
    59461 taaatatgaa caaacgggtc gggtgaccta gctctttcag cgctttagcg acttcttcca
    59521 actgcatatg acacccctct gtctggttga tatttcgata ataccagaaa tatcttgatc
    59581 tgaatttcat ttcgattatt ctggaaatat agatttatcc aatgaggtta cgtatgagta
    59641 gttggcttgc gatgctgcaa gatgccgcag agatgtttgt gtttctcgcc gtcgagcttt
    59701 cgttgctgtt tatagtgatt agtgccggtg tcagcctgat aagacaaaag gtgccagacc
    59761 ataaaatcca gcagatgatg ggggccagaa aagggagagg ctatctcctg gctgctctgt
    59821 tgggagccgt taccccgttc tgtagttgct cgacaatacc catgttacgt ggattgttgt
    59881 cagcgaaagc cgggt
//
LOCUS       DAZPEP010000009        57430 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZPEP010000009 DAZPEP010000000
VERSION     DAZPEP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.009"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="ACHUT9_000554"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="ACHUT9_000554"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFR1347119.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3123)
                     /locus_tag="ACHUT9_000555"
     CDS             complement(1498..3123)
                     /locus_tag="ACHUT9_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1347120.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3582..3668)
                     /locus_tag="ACHUT9_000556"
     tRNA            complement(3582..3668)
                     /locus_tag="ACHUT9_000556"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3632..3634),aa:Leu,seq:gag)
     gene            complement(3683..4015)
                     /gene="secG"
                     /locus_tag="ACHUT9_000557"
     CDS             complement(3683..4015)
                     /gene="secG"
                     /locus_tag="ACHUT9_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFR1347121.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="ACHUT9_000558"
     CDS             complement(4243..5580)
                     /gene="glmM"
                     /locus_tag="ACHUT9_000558"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFR1347122.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5573..6421)
                     /gene="folP"
                     /locus_tag="ACHUT9_000559"
     CDS             complement(5573..6421)
                     /gene="folP"
                     /locus_tag="ACHUT9_000559"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFR1347123.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="ACHUT9_000560"
     CDS             complement(6511..8445)
                     /gene="ftsH"
                     /locus_tag="ACHUT9_000560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFR1347124.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="ACHUT9_000561"
     CDS             complement(8545..9174)
                     /gene="rlmE"
                     /locus_tag="ACHUT9_000561"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFR1347125.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9300..9593
                     /gene="yhbY"
                     /locus_tag="ACHUT9_000562"
     CDS             9300..9593
                     /gene="yhbY"
                     /locus_tag="ACHUT9_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFR1347126.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9749..10225)
                     /gene="greA"
                     /locus_tag="ACHUT9_000563"
     CDS             complement(9749..10225)
                     /gene="greA"
                     /locus_tag="ACHUT9_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFR1347127.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10473..11906
                     /gene="dacB"
                     /locus_tag="ACHUT9_000564"
     CDS             10473..11906
                     /gene="dacB"
                     /locus_tag="ACHUT9_000564"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1347128.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            11961..12068
                     /locus_tag="ACHUT9_000565"
                     /pseudo
     CDS             11961..12068
                     /locus_tag="ACHUT9_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="ACHUT9_000566"
     CDS             complement(12092..13264)
                     /gene="cgtA"
                     /locus_tag="ACHUT9_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFR1347129.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13280..14245)
                     /locus_tag="ACHUT9_000567"
     CDS             complement(13280..14245)
                     /locus_tag="ACHUT9_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1347130.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="ACHUT9_000568"
     CDS             complement(14372..14629)
                     /gene="rpmA"
                     /locus_tag="ACHUT9_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFR1347131.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="ACHUT9_000569"
     CDS             complement(14650..14961)
                     /gene="rplU"
                     /locus_tag="ACHUT9_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFR1347132.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15220..16191
                     /gene="ispB"
                     /locus_tag="ACHUT9_000570"
     CDS             15220..16191
                     /gene="ispB"
                     /locus_tag="ACHUT9_000570"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFR1347133.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHUT9_000571"
     CDS             16419..16697
                     /gene="sfsB"
                     /locus_tag="ACHUT9_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFR1347134.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHUT9_000572"
     CDS             complement(16745..18004)
                     /gene="murA"
                     /locus_tag="ACHUT9_000572"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFR1347135.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHUT9_000573"
     CDS             complement(18059..18313)
                     /gene="ibaG"
                     /locus_tag="ACHUT9_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFR1347136.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHUT9_000574"
     CDS             complement(18473..18766)
                     /gene="mlaB"
                     /locus_tag="ACHUT9_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFR1347137.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHUT9_000575"
     CDS             complement(18766..19401)
                     /gene="mlaC"
                     /locus_tag="ACHUT9_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFR1347138.1"
                     /translation="MFKRLMMVALLVIAPLSAATEADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHUT9_000576"
     CDS             complement(19420..19971)
                     /gene="mlaD"
                     /locus_tag="ACHUT9_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFR1347139.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHUT9_000577"
     CDS             complement(19976..20758)
                     /gene="mlaE"
                     /locus_tag="ACHUT9_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFR1347140.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHUT9_000578"
     CDS             complement(20766..21575)
                     /gene="mlaF"
                     /locus_tag="ACHUT9_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFR1347141.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21785..22762
                     /locus_tag="ACHUT9_000579"
     CDS             21785..22762
                     /locus_tag="ACHUT9_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFR1347142.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHUT9_000580"
     CDS             22776..23762
                     /gene="kdsD"
                     /locus_tag="ACHUT9_000580"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFR1347143.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHUT9_000581"
     CDS             23783..24349
                     /gene="kdsC"
                     /locus_tag="ACHUT9_000581"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFR1347144.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24346..24921
                     /gene="lptC"
                     /locus_tag="ACHUT9_000582"
     CDS             24346..24921
                     /gene="lptC"
                     /locus_tag="ACHUT9_000582"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFR1347145.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24890..25447
                     /gene="lptA"
                     /locus_tag="ACHUT9_000583"
     CDS             24890..25447
                     /gene="lptA"
                     /locus_tag="ACHUT9_000583"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFR1347146.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25454..26179
                     /gene="lptB"
                     /locus_tag="ACHUT9_000584"
     CDS             25454..26179
                     /gene="lptB"
                     /locus_tag="ACHUT9_000584"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFR1347147.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHUT9_000585"
     CDS             26227..27660
                     /gene="rpoN"
                     /locus_tag="ACHUT9_000585"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFR1347148.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27683..27970
                     /gene="hpf"
                     /locus_tag="ACHUT9_000586"
     CDS             27683..27970
                     /gene="hpf"
                     /locus_tag="ACHUT9_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFR1347149.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHUT9_000587"
     CDS             28088..28579
                     /gene="ptsN"
                     /locus_tag="ACHUT9_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFR1347150.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHUT9_000588"
     CDS             28625..29479
                     /gene="rapZ"
                     /locus_tag="ACHUT9_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFR1347151.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29476..29748
                     /gene="npr"
                     /locus_tag="ACHUT9_000589"
     CDS             29476..29748
                     /gene="npr"
                     /locus_tag="ACHUT9_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFR1347152.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHUT9_000590"
     CDS             29962..30594
                     /gene="yrbL"
                     /locus_tag="ACHUT9_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFR1347153.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHUT9_000591"
     CDS             complement(30591..31319)
                     /gene="mtgA"
                     /locus_tag="ACHUT9_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFR1347154.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHUT9_000592"
     CDS             complement(31316..31969)
                     /gene="elbB"
                     /locus_tag="ACHUT9_000592"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFR1347155.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHUT9_000593"
     CDS             complement(32199..34535)
                     /gene="arcB"
                     /locus_tag="ACHUT9_000593"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFR1347156.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34631..35560)
                     /locus_tag="ACHUT9_000594"
     CDS             complement(34631..35560)
                     /locus_tag="ACHUT9_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFR1347157.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36235..40695
                     /gene="gltB"
                     /locus_tag="ACHUT9_000595"
     CDS             36235..40695
                     /gene="gltB"
                     /locus_tag="ACHUT9_000595"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFR1347158.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40708..42126
                     /gene="gltD"
                     /locus_tag="ACHUT9_000596"
     CDS             40708..42126
                     /gene="gltD"
                     /locus_tag="ACHUT9_000596"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFR1347159.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42310..42714
                     /locus_tag="ACHUT9_000597"
                     /pseudo
     CDS             42310..42714
                     /locus_tag="ACHUT9_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417687.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
     gene            42806..43111
                     /locus_tag="ACHUT9_000598"
     CDS             42806..43111
                     /locus_tag="ACHUT9_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1347160.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43187..43651)
                     /gene="nanQ"
                     /locus_tag="ACHUT9_000599"
     CDS             complement(43187..43651)
                     /gene="nanQ"
                     /locus_tag="ACHUT9_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFR1347161.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="ACHUT9_000600"
     CDS             complement(43648..44523)
                     /gene="nanK"
                     /locus_tag="ACHUT9_000600"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFR1347162.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44520..45209)
                     /locus_tag="ACHUT9_000601"
     CDS             complement(44520..45209)
                     /locus_tag="ACHUT9_000601"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFR1347163.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="ACHUT9_000602"
     CDS             complement(45257..46747)
                     /gene="nanT"
                     /locus_tag="ACHUT9_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFR1347164.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="ACHUT9_000603"
     CDS             complement(46857..47750)
                     /gene="nanA"
                     /locus_tag="ACHUT9_000603"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFR1347165.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="ACHUT9_000604"
     CDS             complement(47872..48663)
                     /gene="nanR"
                     /locus_tag="ACHUT9_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFR1347166.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49043..50365
                     /gene="dcuC"
                     /locus_tag="ACHUT9_000605"
     CDS             49043..50365
                     /gene="dcuC"
                     /locus_tag="ACHUT9_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1347167.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="ACHUT9_000606"
     CDS             complement(50408..50905)
                     /gene="sspB"
                     /locus_tag="ACHUT9_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFR1347168.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="ACHUT9_000607"
     CDS             complement(50911..51549)
                     /gene="sspA"
                     /locus_tag="ACHUT9_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFR1347169.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="ACHUT9_000608"
     CDS             complement(51944..52336)
                     /gene="rpsI"
                     /locus_tag="ACHUT9_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFR1347170.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="ACHUT9_000609"
     CDS             complement(52352..52780)
                     /gene="rplM"
                     /locus_tag="ACHUT9_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFR1347171.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="ACHUT9_000610"
     CDS             complement(52999..54126)
                     /gene="zapE"
                     /locus_tag="ACHUT9_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFR1347172.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54320..54718
                     /gene="zapG"
                     /locus_tag="ACHUT9_000611"
     CDS             54320..54718
                     /gene="zapG"
                     /locus_tag="ACHUT9_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFR1347173.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54872..56239
                     /gene="degQ"
                     /locus_tag="ACHUT9_000612"
     CDS             54872..56239
                     /gene="degQ"
                     /locus_tag="ACHUT9_000612"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFR1347174.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56329..57396
                     /gene="degS"
                     /locus_tag="ACHUT9_000613"
     CDS             56329..57396
                     /gene="degS"
                     /locus_tag="ACHUT9_000613"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFR1347175.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14259 a        15304 c        14261 g        13606 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaacttcta gcaaatttgt tgaatactgt
     3121 catccagaaa tccaagttca caaccaatat aaacaattat caacaatgtt caattttata
     3181 tcagacatgt ttttcaactc tgatccatat cagaccgaaa cactgttagt tttttctttt
     3241 taaatgatgc ggccattgcg ttctcagtaa ttaaataaca ttatcaatgc gtctctaaac
     3301 attacgcaga acgtagccat cagtagttat actgacagga gcaggatggt ttatcgagaa
     3361 gtaaacatat ccgtattcgt accggaactg gagttgacac aataaagtgc caattatgtc
     3421 agcagaaggg aaaagtttat aactaaggcg taccggcacc atcgtttcaa ggtaccagct
     3481 acgagtaaag caactggacg agatacagat acctgacaac cattcctcag accaggacca
     3541 aaacgaaaaa agacgctttt cagcgtctct tttctggaat ttggtaccga ggacgggact
     3601 tgaacccgta agccctattg ggcactacca cctcaaggta gcgtgtctac caattccacc
     3661 acctcggcac ggatactact ttttagttcg ggatatcgct ggtcggctta gccggagcag
     3721 ctggctgagt ttgttcggtt ttcgccggtg cactcagatt ttcccattcg ctacctttat
     3781 tggttttgtt gctattgatg ttacccagca ccagactgat gatgaagaat aacgttgcca
     3841 gcagcgccgt catgcgggtc atgaagttac cagaaccact tgaaccaaac agcgtagcgg
     3901 aagcgcctgc tccgaaggag gctcccatat cagcgccttt accttgctgc agcatgatca
     3961 gaccaacaag gccaattgcc acaataagga aaactaccaa aagagcttcg tacataatca
     4021 acctgttcct tgcggagttg ccgcgtacca atgcttcaac caataaagcg ggagttttta
     4081 atctttccca ctgaagtggg tgtgaatact aaccaaagcg aatgaccttc gcaagggcaa
     4141 ttttatcgca ttgtatcaac tgcggaaaaa aacagcaaaa ccgatgtgtt cgctgagaaa
     4201 aaaggcgaca atcgccgcct ttttagccag ttatttaccg ttttaaacgg cttttactgc
     4261 atcggcgata cggtgtgcaa attcagttac ctgcgcttcg tcttcacctt ctaccatcac
     4321 gcgaattaac ggttcggtgc cggatttacg caacagcaca cgtccacggc tacccagcgc
     4381 cgcttcaact tcggcggtca cagctttaac tgactcatgc tcaagcggat cgccgctacc
     4441 tgcggtgtaa cgcacgttaa ccagaatctg cgggaacatt ttcatgccgc tgcaaaggtc
     4501 gtgcaggctc atatggttac gtgccatcgc cgccagcacc tgcaagccag caacgatgcc
     4561 gtcaccggta gtggttttat ccagcaggat cacatgaccg gaattctctg caccgatacg
     4621 ccagcctttc tcctgcattt tttccagtac gtagcggtca cccactttcg cgcgcgcaaa
     4681 tggaattcct aactgtttca acgccagctc aagccccatg ttactcatca atgtacccac
     4741 agcaccacca cgcagctggc cctgacgaag accttcacgc gcgatgatat acatgatctg
     4801 atcaccatcg actttattgc cttcatggtc aaccataatc acgcgatcgc cgtcgccgtc
     4861 gaaggcaata ccgagatccg ctttttcagc cagcacacga gcctggagcg cgcgaacgtc
     4921 ggtagccccc acttcggcat tgatgtttac accgttcggc tcacaaccga tagcgataac
     4981 gttcgccccc agttcacgca gcacgttcgg cgcgatgtga taagtcgcac cgtttgcgca
     5041 atccaccaca atcttcagtt cgctgaggct aagttcgttc gggaacgtgg ctttgcaaaa
     5101 ctcgatatag cgacccgcag catcaacgat acggctggct ttacccagtt ctgccgaatc
     5161 aacacagctg atctcttttt ccatttccgc ttcgatggcc tcttctaccg catccggtag
     5221 tttggtgccg tcgatagaga aaaatttaat gccattatcg tagaacgggt tatgcgatgc
     5281 agatatcaca attccggcct ctgcgcggaa ggtacgcgtc agataagcca cggccggtgt
     5341 tggcatcggg ccagtgaaga gtgcggaaag gcccgctgcc gccagacccg cttccagtgc
     5401 tgactccagc atatagccag aaatacgcgt gtctttacca ataatgatct tacgggagcc
     5461 gtggcgcgcc agcactttac ccgcggccca acccagctta agcacaaaat caggtgtgat
     5521 cggcgcatcc cctacacgac cacgaatccc atcggtaccg aaatatttac gattactcat
     5581 agcgtttgtt ttcctttgca gacagagtgg cttccaccac ccgcatcgct tctacggttt
     5641 ctttgacgtc atgaacacga ataatgtgcg cgccttgcat tgcggcaatg accgcacagg
     5701 ccagactacc gctcaggcgc tcggacggcc ccacgttcag cagctgccca atcatcgatt
     5761 ttcgtgacat acctaccaac agcggcaggt tgaaatggtg aaattcagcc aggcgcgcca
     5821 gtaatgaata gttatgggag agatttttac cgaaaccgaa tccggggtcg agcaacaatt
     5881 tctcttttgc gatacccgcc tgctcgcaac gtgctatttg ctcaataaag tagcgattca
     5941 cttctgcaaa gacatcgtca tacttcggag cttcctgcat ggtttttgga tttccctgca
     6001 tatgcatcag acaaaccggt aaaccggttt ctgcagccgc ctccagagcg ccaggttcgg
     6061 aaagggagcg gatatcgtta ataatgtgag cgccaacttt cgctgactca cggatgactt
     6121 ctggtttgga tgtatctacc gagatccaga cttcgaagcg ttgagcaatt gcctcaacca
     6181 caggaataac acgttgcaac tcttcttcaa cgctaacttc cgccgcccct gggcgcgtgg
     6241 actcgccacc aacgtcaatg atcgtcgcgc cagcgttgat catcagattc gcatgtttca
     6301 ccgcatctat cagcgagtta tgcgtgccac catccgaaaa ggaatcaggc gtgacgttga
     6361 ggatccccat tacgtgagga tggctaaggt ccagtgaagt gccctgggca aagagtttca
     6421 tggtgttatc cctggtatga attgataaga aaaaaccccg gagcacgccc cggggttttc
     6481 ggtacaaata cagtcatctg atgcgggaac ttacttgtcg cctaactgct ctgacatggt
     6541 gttacccggg ttcggcgtac gcggttcatc aaccggacga ggagcctttg gactaccatt
     6601 gtcgccagaa ttgttagaag cgcctggttc ttcccagccc gctggcggac gtacatcgcg
     6661 acgtgccatc aggtcatcaa tctgcggtgc gtcgatagtc tcatatttca tgagagcatc
     6721 tttcatcgca tgcagaatat ccatattgtc ggtcagaagc tgacgcgcac gattatagtt
     6781 acgctcaatc agtgctttca cttcctggtc gatgatacgt gcagtttcat cggacatatg
     6841 tttcgctttc gctacgctac ggccgaggaa cacttcacct tcttcttccg cgtacagcag
     6901 tggacccaat ttctcagaga agccccactg agtcaccatg ttacgcgcca ggttggtcgc
     6961 aactttaata tcgttggacg caccggtaga aacatgttcc ggcccgtaga tgatctcttc
     7021 tgccagacga ccaccgtaca gcgtagaaat ctggctttcc agtttctgac ggctggcgct
     7081 gattgcgtcg ccctcaggca agaagaaagt cacacccagc gcacgaccgc gtgggataat
     7141 cgtcactttg tgcaccggat cgtgttccgg caccaggcga ccgataatcg catgacccgc
     7201 ttcgtggtaa gccgtcgatt ctttctgcgc ttccgtcatc accatggagc gacgttccgc
     7261 acccatcatg attttatctt tcgctttctc gaactcaacc atcgacacaa cgcgtttgtt
     7321 gccacgagca gcgaacagtg ccgcttcgtt taccaggttc gccaggtcag caccggagaa
     7381 accaggagta ccacgggcaa tgattgccgc gtcgatatcg ggtgccaatg gtacgcgacg
     7441 catgtgaact ttcaggatct gctcacgacc gcgaacatct ggcaagccaa ccacaacctg
     7501 acggtcgaaa cggccaggac gcagcagggc cgggtcaaga acgtccggac ggttagtcgc
     7561 ggcgataacg atgatacctt cgttaccttc gaagccatcc atctcaacca gcatctggtt
     7621 cagagtctgt tcacgttcat cgtgaccacc gcccagacca gcgccacgct ggcggcctac
     7681 ggcgtcgatt tcatcgataa agatgatgca cggtgccgct ttcttcgcct gttcgaacat
     7741 gtcacgaaca cgggatgcac ccacaccgac gaacatttct acgaagtcag aaccggagat
     7801 agtaaagaac ggaactttcg cttcgcctgc aatcgctttc gccagcagcg ttttaccggt
     7861 acccggagga ccgaccatca ggacgccttt cgggatctta ccgccgagtt tctggaagcg
     7921 gctcggctcg cgcagatatt caaccagttc agcaacttct tcttttgctt cgtcgcagcc
     7981 cgcaacgtca gcaaaggtcg ttttgatctg atcttccgtc agcatgcgcg ctttgctctt
     8041 accaaacgac atggcacctt tgccaccgcc gccctgcatt tgacgcatga agaagatcca
     8101 cacaccaatc agcaacagca tcgggaacca ggagatgaag atagaagcca gcaggcttgg
     8161 ttcttcaggc ggttcaccga caaccttgac gttcttggtc aacaggttat ccagtaattt
     8221 cggatcctga accggaatgt aagtggtata acggttacta tctttcttgg taacgttgat
     8281 ttcacgtccg ttgatacgcg cttcacgaac ctggtcgtta ttgacctctt gtaggaaggt
     8341 agagtaatcc accttacggc cattagactc gctgggccca aagctctgga atactgacat
     8401 cagcacaacg gcaatgacca gccagagtat taggtttttc gccatgtcac tcaagggatt
     8461 aacctcttat tacaactgtg ttaaaaacag cgtcaggata ctctatatcc agcatctttc
     8521 aaactttcgt ctgaaatctc ccggttaggg tttacgcccg gtcgctacaa tatacacttc
     8581 ccgcgaacgt gcacgagaag agtccggctt acgaactttg accttcgtaa acagggagcg
     8641 aatttccctt agatactcat cgaaaccttc gccctggaac accttcacta caaaactgcc
     8701 acctggcgct aatacatcac gacacatttc tagcgccagt tccaccagat acatggcacg
     8761 ggggatatcc accgccggtg ttccgctcat gtttggtgcc atatcggaca tgacaacctg
     8821 gactttgctg tcgccaacgc gctccagcag tgctttcatc accagttcat cacgaaaatc
     8881 gccctgaaga aagtccacac caacgatagg atccataggt aaaagatcgc aagcgatgat
     8941 gcggcctttg ccgccaattt gggtgaccac atattgtgac caaccacccg gagcagcacc
     9001 aaggtcgaca accgtcattc ccggtttaaa gagtttgtca ctttgctgta tttcatcaag
     9061 tttaaaccag gcacgggaac gtaacccctt tttctgtgcc tgttgaacat atttatcgct
     9121 aaagtgttcc tgaagccagc ggctggagct ggcagaacgc ttcttacctg tcatttaact
     9181 ttcccatggg gataactcat cgtaaccaat tgcgtaaatt tttacgcgcc tatttggtga
     9241 tatatgggag atggcggtag aatgacccat tttcaatccc aacgtaagca aaaaatacga
     9301 tgaatctgag tactaaacaa aaacagcacc tgaaaggtct ggcacatccg ctcaagccag
     9361 ttgttctgct tggcagtaat ggtttgaccg aaggggtgct ggccgagatt gaacaagcgt
     9421 tagagcacca tgaactcatc aaggtgaaaa tcgccaccga agatcgcgaa acaaaaacct
     9481 tgatcgtgga agctatcgtg cgcgaaaccg gcgcctgtaa tgtacaggtc atcggtaaaa
     9541 cgctggtgct ttatcgccca actaaagaac gtaaaatctc gctgccacgc taagattatc
     9601 ctaaagttac acacatttgc tgtgtaaaac gaggggtttt ccgcaggcag gagagcaaaa
     9661 tgccacgctc tgttcgttga taaaaggccg catagcggcc tttttccttt ctttacaata
     9721 catcaacatc ttgagtattg ggtaattctt acaggtattc caccttaatt acttcaaatt
     9781 ctacttcgcc gcccggcgtt ttgatgacca caacatcatc ttcttctttg ccgatcaggc
     9841 cacgagcaat aggcgagttt acagaaatca ggttttgttt aaagtcagct tcgtcatcgc
     9901 caacgatgcg ataagtctgt tcttcgtcag aatccagatt cagcaccgtt acggtagcac
     9961 caaaaataac gcgcccattg ttaggcattt tggtgacatc aatcacctgc gcgtttgaca
    10021 gcttggcttc gatgtctttg atacggcctt cgcagaagcc ctgctgttca cgagctgcgt
    10081 ggtattcggc gttttctttc aggtcgccat gctcacgcgc ttccgcgata gcagcaatga
    10141 tttcaggacg gcgcacagat ttcagaaaat ccagctcttc gcgtaatttt tcagcgccgc
    10201 gtaaggtcat cggaatagct tgcatttgtt atacctcttg aatattcctg atggggcaag
    10261 tcttcaccca ccccggctgt tcggcctgcc cggcataact cctcaccggg accagaagca
    10321 aaaaaatacc gacccaggta caagtcccag gtcagctaca attcacattt tgatagtcat
    10381 tttaccctga agttcccgaa gggtcatcgt ttactttata gggcgttgcg ccgtagtatg
    10441 acggctcgat tccaggttgt tagcgcgaga ttatgcgatt ttccagattt atcatcggat
    10501 tgaccagctg tatagcgttc agtgttcagg ccgcaaatgt tgatgagtac attactcaac
    10561 tccccgctgg tgccaacctt gccctgatgg tgcaaaaagt cggcgcgtcg gcccccgcta
    10621 ttgattacca cagtcagcag atggcgctgc ctgccagtac ccagaaagtg attactgcgc
    10681 tggcggcgtt gattcaactc ggccccgatt ttcgttttac cacgacgctt gaaaccaaag
    10741 gcaatgtgga aaacggcgta cttaagggtg acttagtggc gcgatttggt gccgatccga
    10801 cgttaaaacg tcaggatatt cgcaatatgg tcgcgacttt gaaaaaatct ggcgtcaacc
    10861 aaatcgatgg caatgtgttg atagatacct ccattttcgc cagccacgat aaagcccccg
    10921 gctggccatg gaatgacatg acacaatgct ttagcgctcc gcctgccgcc gccatagttg
    10981 accgcaactg tttctccgtc tcgctctaca gtgcccaaaa acccggtgat atggccttta
    11041 tacgcgtggc atcttattac cccgttacga tgttcagcca ggtacgcacc ctcccccgtg
    11101 gttctgccga agcgcaatac tgcgaactgg atgtcgtacc aggcgacctg aaccgcttta
    11161 cgctgacggg atgcctgcca caacgttctg agccgctccc gttggctttt gccgtgcagg
    11221 atggagccag ttatgccggt gcaattctga aagatgagtt aaaacaggcg ggtatcacct
    11281 ggagcggaac actgctgcgc cagactcagg ttaacgaacc tggaacggta gttgccagta
    11341 aacagtcggc cccgctgcac gatctgctta agattatgct gaaaaagtcg gataacatga
    11401 tcgccgatac ggttttccgc atgataggcc atgcgcgctt caatgtacct ggtacatggc
    11461 gggccgggtc ggacgctgtg cgtcagatcc tgcgccagca agccggtgtc gatattggaa
    11521 acaccattat tgccgatggt tcagggcttt cgcggcataa cctgattgcc cccgccacca
    11581 tgatgcaggt gctgcaatac attgcccaac acgacaatga acttaacttt atctccatgc
    11641 tgccgctggc gggctatgac ggctctttgc agtaccgtgc aggtctgcat caggcgggcg
    11701 tggatggaaa agtctcagcg aaaaccggtt cgttgcaggg ggtatataac ctggcgggat
    11761 tcattaccac agcgagcggg caacgaatgg cgtttgtgca atatctttct ggctatgcag
    11821 tagaacctgc ggatcagcgt aatcgccgta ttccgttagt gcgttttgaa agccgtttgt
    11881 ataaagatat ttatcagaac aattagtcaa aaagaaaccc cggcacatgg ccggggcttc
    11941 agattattga caaagtgcgc tttgtttatg ccggatgcgg cgtaaacgcc ttatccggcc
    12001 tacaaaatcg tgcaaattca acatattgca attctcttgt aggcctgata agcgtagcgc
    12061 atcaggctga tttggcgttt atcatcagtg attaacgctt gtaaatgaac tcaacgcctt
    12121 cttcgtcgtc ttcgtcccag tcgtcatccc agtcttcatc atcctcttca gcaatctctt
    12181 caagctgctg gcgatgataa tcgtcccaca tgaattcgac tttctctggc tgtttcgctt
    12241 cttcagcctg cacgaccggg ttttcaagga taaaggtcat cacatcccag cagagatctt
    12301 tcacgcccag tccgctcgcc gcagagatca gataatattt atcttcccag cccagcgctt
    12361 cagcaatcgc tttcgctttc tcttcagctt ctgccttatc cagcagatcg atcttattga
    12421 acactaacca acgcggtttc gccgccagat cctggctgta tttttccagc tcgctgataa
    12481 taatacgcgc gttttcaacc ggatcggtgc cgtcaatcgg atcgatatcg atgaggtgca
    12541 acaggacgcg gcaacgttcc aggtgcttca ggaagcggat acccagacct gcgccttccg
    12601 cagcgccttc aatcagtcct ggaatatcgg caacaacgaa gctcttttcg ttgtccattc
    12661 gtaccacacc cagacttggc accagagtgg taaacggata atccgccact ttcggtttag
    12721 ccgccgatac cgcgcgaata aaggtcgatt tacccgcgtt tggcatcccc aacataccga
    12781 cgtcagccag cagcatcagc tccagcagca gctcacgctt atcacccggc gtaccgttgg
    12841 ttttctgccg tggtgtacgg ttaacggacg atttgaaacg ggtattgccc agaccgtgcc
    12901 agccgccctt agcaaccagc agacgctgac cgtgtttagt catatcgccc atggtttcac
    12961 cagtaccctg gtcgattaca cgcgtaccta ccggcacttt aatcgtcacg tctttaccgc
    13021 gcttaccggt acaatcgcgg cttgcgccgt tctgaccgcg ctctgcacgg aaagattttt
    13081 caaaacgata atcgataagc gtgttcaggt tttcgtcggc ttccatccat acgtcaccac
    13141 catcaccacc gtcgccgcca tccgggccgc ctttcggaat atacttttcg cggcggaagc
    13201 tcacgcaacc attaccgcca tcacctgcaa cgaccagaat cgatgcttca tcaacaaact
    13261 tcattttatt ctccataaat cattcgcctg cgcggggttg cgataccacc gttgtatgct
    13321 tacgtaatcc gccccaaata cgatgaccaa tggcggaata catcgcgccc gcaaccacga
    13381 caaacgcacc gagataacct aaaaggttta acatcggtct ggcgaagaaa tcgggccagg
    13441 ccagtgataa aagatctgaa aaaaacagcg taaacagtgg ggtgagcgtg atgatcgcgc
    13501 tcacctgcgc tgcctgccag cgagccatcg cttccgccag ggcgccatat cctaccaatg
    13561 tattcagtcc gcaaaaaatt aaacatgcga gctgccagtg gctaagctgc gctatcactc
    13621 caggctttgc cagagggaag agcgcaattg tacataaagt gtacagtaaa aacaggatct
    13681 gcggtgaggc cagccgacgc aataaaacct tttgcgccac gccataactc acccaaaccg
    13741 tcgccgcacc gaccccaaag ataactcccc aggtgtaatc ggtgagcttt gtaaatatct
    13801 cgaccagact ggtgttaaaa aacatcacca ggccgctcag gagcatcaat gcccctacaa
    13861 cctgagtgct gcgcattttc tctttcagga taaatacgct ggcaaccatc atgccaactg
    13921 gcgagagttg cccaatcacc tgcgaagcgg tcggactcag gtattgcaag gatgagctga
    13981 acaggatgaa gttcccaaac agcccggcgg tcgccactgc caacaaaatc aaccagcgtg
    14041 gcttacgaaa cacgcgtaat ggcggcaacc tcttcttcac cgcaagaatg gcaccaaggc
    14101 caatactcgc catcaagaaa cggtaaaaca cgattgtcgg aggttccatc acctccagca
    14161 cctgcttcat tgcgattggc aacgcccccc agcaaattgc tgtggtgagc gccaaaagaa
    14221 tgccaatgcc tgcctgctgc ttcataccgt attccctgca aagcgcattt gcggattaac
    14281 gtcgcaccat gcgcattttt accggttatc gaatgtaaaa agccccgcaa cgtgttgcgg
    14341 ggctttcatc cgttaccggg acgcgaaaaa cttattcagc ttcgatgctg ataaatttac
    14401 ggtttttcgg gcctttaact tcgaatttca ctttaccgtc tgctttagca aacagagtgt
    14461 ggtcacgacc gcaacctacg ttagcgccag cgtggaattt ggtaccacgt tgacgaacga
    14521 tgatgctacc cgccagaacg gattcgccac cgaaacgctt aacgcccagg cgtttagctt
    14581 ctgaatcgcg accgttacgt gtggagccgc cagccttttt atgtgccatt tgaaatctct
    14641 cctcaggtct taggcgctga tgccagtaat tttcacatca gtgaaccact gacgatggcc
    14701 ctgctgctta cgatagtgtt tacgacgacg aaacttaacg attttaactt tctcgccacg
    14761 accgtgagca acaacttcag ctttgattac gccgccatca acgaaaggaa cgccgatttt
    14821 gacttcttca ccgtttgcga tcatcagcac ttcagcgaac tcaacagttt cgccagttgc
    14881 gatgtccagc ttttccaggc gaacggtctg accttcgctt actcggtgtt gtttaccacc
    14941 actttggaaa accgcgtaca tataaaactc cgcttccgcg cacacctttt caatgattca
    15001 gagtgcgcta taaatattca caatagggcg cgaatattac gcaaaacgca cgcctttgac
    15061 aagtgctaca gtcaatacac gaagaaaaaa aacacaactt gtacggtaac gtttatctgt
    15121 gccatttttt cagtacaatc accctatatt cctaaccata aaccctaagt tgcctttgtt
    15181 cacagtaagg taatcggggc gaaaagcccg gcttttgcga tgaatttaga aaaaatcaat
    15241 gagttaaccg cgcaagatat ggcgggtgtt aatgcggcaa tccttgagca gcttaattcc
    15301 gacgtccaac tgatcaatca gttaggctat tacatcgtca gcggcggcgg taaacgtatt
    15361 cgtccgatga ttgctgtact ggctgcacga gctgttggct atgagggaaa tgcgcatgtc
    15421 accattgctg ccctgatcga gtttatccac acggcgactc tgctacacga cgacgttgtg
    15481 gatgaatcag atatgcgcag gggtaaagct accgccaacg ccgcatttgg caatgccgcc
    15541 agcgtgctgg taggcgattt tatttatacc cgcgcgttcc agatgatgac cagcctcggt
    15601 tcactcaaag tgctggaagt catgtcagaa gctgtaaacg tcatcgcaga aggtgaagtt
    15661 ctgcaactga tgaacgttaa cgatccggac atcactgaag aaaactacat gcgcgttatc
    15721 tatagcaaaa ccgcgcgtct gtttgaggct gccgcgcagt gttccgggat tctggctggc
    15781 tgtacgccgg aggaggagaa aggcctgcag gattatgggc gctatctcgg cactgctttc
    15841 cagttgatcg acgatttact cgattacaat gccgatggcg aacagttagg taaaaatgtc
    15901 ggcgacgatc tgaacgaagg taaaccgacg ctgccgctgc tgcatgcgat gcatcatggc
    15961 acaccagaac aggcacagat gatccgtacc gccatcgaac agggtaacgg tcgccatctt
    16021 ctggaaccgg ttctggaagc aatgaacgct tgtggatctc ttgaatggac gcgtcagcgt
    16081 gccgaggaag aagcagacaa agccatcgca gcattacagg tgctcccgga caccccttgg
    16141 cgagaagcac tcatcggcct cgcgcacatc gctgttcaac gcgatcgtta atcccctccc
    16201 ttcatcccgc gcagtgcgcg ggatgattcc agtaaattcc ataaaaacac taattcagcg
    16261 ctataacacc tgaaaaaaag ggcgatgaat ctttatatat tctgaatatt cacactcttt
    16321 acaggaactt tttagagcaa taggccatca ggagtatagt gatgcccgac agaagaagtg
    16381 ttctgaatga aagcgactaa cttaaggagt gaggaaagat ggaaagtaat ttcattgact
    16441 ggcatcccgc tgacatcatt gcgggtttgc gcaagaaggg aacgtcaatg gcggcggagt
    16501 ctcgcagaaa tggtttgagt tcctcaacgc tggcaaacgc attatcgcgc ccatggccga
    16561 aaggagagat gattattgcg aaagccctgg gaactgaccc ctgggttatc tggccatcac
    16621 gctaccatga tccgcagacc catgagttta tcgacagaac gcagttgatg cgtagctaca
    16681 ctaaaccgaa aaaatgagtg gtctggcggt agccccgcga acggggctgc cagctctcag
    16741 acgattactc gcctttcaca cgctcaatat ttgcacctaa agcgcgcagt ttgtcttcaa
    16801 tgcgttcgta gccacgatcg atgtgataaa tacgatcaac caccgtcgtc ccttccgcaa
    16861 tacagccagc cagcaccagg cttgctgatg cacgcagatc ggttgccata acctgtgcgc
    16921 cagaaagttt ttcaacaccg tgacaaataa cggtattgct ttcgatttcg gcatgcgcgc
    16981 ccatacggct aagctccggc acatgcataa agcggttttc aaagaccgtt tcggtgatga
    17041 atccggtccc ttctgccacc aggttcaaca gcgtgaactg ggcctgcata tcggtcggga
    17101 atgccggatg cggcgcggta cgtacgttaa cagccttcgg acgtttgcca tgcatatcca
    17161 ggctaatcca gtcttcgccg acttcgatgt ccgctccagc gtcacgcagt ttcgccagca
    17221 cggcgtcgag agtatctggc tgcgcgttac ggcagataat tttgccgcga gaaatcgccg
    17281 ccgccaccag gaaagtaccg gtttcgatac gatccggtag cacgcgatag acaccgccgc
    17341 ctaaacgttc cacaccttcg atgacgatac gatcggtgcc ctgaccgcta attttcgcac
    17401 ccagcgtaat caggaagttc gcggtatcga cgatttccgg ttcacgcgct gcgttttcaa
    17461 taatcgtggt accttccgcc agggttgcag cacacatgat ggtcaccgtt gcgccaacgc
    17521 tgactttatc catcacgata tgtgcgcctt tcagacgacc atcgacggaa gctttaacgt
    17581 aaccttcttc cagtttgatg gtcgcgccta attgttcgag gccagaaatg tgtagatcaa
    17641 ccggacgcgc accgatcgta caaccgccag gcagtgaaac ttgcccctga ccaaagcgcg
    17701 ctaccagcgg ccccagtgcc cagatagaag cacgcatggt tttaaccaga tcgtaaggtg
    17761 cgcagaatac attaacgtcg cgggcatcaa tatgcacaga accattacgt tctactttcg
    17821 cacccagctg gcttagcagc ttcattgatg tatcgacgtc tttcagtttt gggacgttct
    17881 ggatctctac cggttcttcc gccagtagag cggcaaaaag gataggcaga gcagcatttt
    17941 tagcgccgga aattgtgact tcgccctgga gcttcgttgg cccctgaaca cgaaatttat
    18001 ccatttagtt tgttctcagt taacaattca tatccgctac cggcgaatcg cccatagctc
    18061 aaaagccgtt cagtttgcga tcgcgcgccc actccgcagg ggtatacgct ttgatcgaca
    18121 cagcatgaat gcggttatcc gcaatatatt ccatcagcgg gccatagacc gtctgctgtt
    18181 ttttaacccg actcatgccg tcaaacaact cacccacggc aataacctga aagtggctgc
    18241 catcgccgga aacgtggact tcctggaggg agagagcgtt catcaacacg ctctgaattt
    18301 cattattttc catgggatct tcaatcatca gttaataaac cagcgaaaca tcttagagca
    18361 aagttgcgct ggcataaata agcaaaaagc ctcgctgata aatcagacaa ggctcgactt
    18421 gcaggcaggt ttgccggaca ggcggttaac gccatatccg gcctgaaaaa atttaacgag
    18481 gcagaacatc agcaggcaaa ttatacaatt tcgcgagggt atacactttg tcgtttaccc
    18541 cctgaagcgt cacgttgttg ccctgctttt tcgccagatc gataagatgg agcagcagtg
    18601 ccagtccacc cgtatccacg cgggacacac ggctaagatc gatgcaggta atccccttca
    18661 ccgcttcctc acgcatttcc caaagcggta gcagaacgtc ctgatccagc tctccggata
    18721 acgccagcgt gtcacccgtc tgcatccagc tcagtgactc gctcattatt ttttctcttc
    18781 cagagtgatt ttctgttgag aaatcgattt cagttgcgca gtcaggccgt cgataccttt
    18841 ggtacgcagc agcgttcccc actcgttttg tttggtggtg atcatactga cgccttcagc
    18901 aatcatgtcg taagcctgcc agtttcccgt ctgggagttt ttacgccact ggaagtccag
    18961 acgcaccggc ggacggccat tcgggtcaat aatggtaacg cgaataggca caatggtttt
    19021 atcgcccagc ggctgttctg gcgcaatctg ataggtttga ccgtgataca tcgccagcgc
    19081 ctgaccgtaa gcctgcttca ggtactcacg gaaagcggca aagtaggctt cacgttgagc
    19141 aggggtcgca ctcttgtaat actggcccag caccagcgca ccggcgtatt tcacctgtac
    19201 gtatggcagc agttcctgat caacaatggt acgcagataa tccgggttgg cccgaatttg
    19261 cggttgctca ttcttcaggc gatcgaacgt tttctgcgcc gcctcgtcca tcagcttata
    19321 aggattggtc tggtctgcct cggttgccgc actcagaggt gcaatcacca gcaaagcgac
    19381 catcattaaa cgtttaaaca tgcgtcggtt ctcctgaaat tatttcgttg tacccacagg
    19441 ttcagtggtt tcattattac ctggcgcagc agctggcgca tcgccactat tcttattgtc
    19501 atcgccttta ctaccgtaaa ggaactgacc aatgagatct tccagcacca tcgcagactt
    19561 agtgtcctga attgtatcgc catccttcag gatagcagtc cccagttccg ggtcttcaaa
    19621 accgacgttt aatgccagat attgttcccc cagcaggccg gaagtacgaa tgctcagcga
    19681 actggtatct ggaatgtggt tataacgttg ttcaatttcc agcgttacgc gcggcagata
    19741 ggttttcggg tccagcgtaa tatccgccac ccgacccaca acaacgccac caatactgac
    19801 cggagagcgc gctttcaggc cgccaatgtt atcgaacgtc gcataaagcg tgtaggtcgg
    19861 ttcagtacgt atggacgtca cgttcgccgc cttcaggcaa acaaacagcg ccgccagcag
    19921 tgctgctaat aaaaagatac ccacccaaat ttcatttttt ttcgtttgca tgaactcaat
    19981 tcccaaacat caatgcggtc agcacaaaat ccagccccag aacagccaga gacgagtgga
    20041 caacggtgcg agtggttgcc cggctaatcc cggcagacgt cgggatggcg tcgtagccgt
    20101 taaacaacga aatccacgtc accgtgatgg cgaacaccac gctcttaatc agacagttga
    20161 ccagatccat acgccagtcg acggcatttt gcattgccga ccagaagaac ccgctatcaa
    20221 tgcctttcca actgacgccg accagagatc cgccccagat ccccacggcg acgaaaataa
    20281 ccgtcaacag tggtaatgaa ataaccccag cccagaaacg gggagaaata acccgacgca
    20341 gcggatccac cgccatcatc tccatactgg agagttgctc tgtagcgcgc atcaggccga
    20401 tttctgcggt tagcgccgaa ccagcacgcc cggcaaacaa caacgcggca accaccggcc
    20461 ccagttcacg cagtagcgat aacgccacca gcatacccag actggtttcc gcactataag
    20521 tggtcagaac cagataacct tgcagcccca acaccattcc gatgaacacg ccagaaacca
    20581 caataatcag catcgacagg acgccgacat tatagagctg gcgcaccagc agcggcgcat
    20641 gtttgcgaaa ttccggtttg ccgaccagcg cattgaataa cattaacccg gcccgcccga
    20701 acgttctcag ggttttaatc cctttatgtc cgagcgacgc cagcgcattt aacagcatga
    20761 gtggcttaac tccctggtaa aagatcagcg tgataatcgc cggcaggata gcggaacgga
    20821 acaggcccgt cagctatccc gtccagaaac tgacgtacgc gcggatcagg attcgcctgc
    20881 aacgcctggg cactgccatg agcgacaatt tttttgtccg ccaggatcca ggcgtgatcc
    20941 gcaatactta acacttccgg cacatcgtga gaaaccacca cacaagtcac gcccagcgcg
    21001 ctgttcagct cagaaatcag cttcaccagt acgcccatgg tgatgggatc ttgcccaaca
    21061 aaaggttcat caaacatgat gagatccggc tccagcgcaa tcgcacgcgc cagcgctgca
    21121 cgccgcgcca tcccaccgga aagttcagaa ggcattagtt tagccgctcc acgcagcccc
    21181 acggcctcca gcttcatcat caccgtacta tgcaacaatg gcgcgggtag ttgggtatgt
    21241 tcgcgcagtg gataggcgac gttgtcaaat acgttcatat cagtgaacaa cgccccggac
    21301 tgaaataaca tgctcatccg tttgcgcact gtatacaggc gcgaacgaga catcgccgga
    21361 atattctcac catcgaaaag gatctcacca tgatctggtg cgatttgccc gccaatcaga
    21421 cggagtagcg tcgttttacc gatgcccgat ggccccatga tcgccgtgat cttccctcgc
    21481 ggcacggtca gggaaatatt atcgaagatg cagcgattgc cacgcgtaaa actgacatcg
    21541 cgcatatcga ctaaattcgc cacagactgc tccataattc acccttcgtc ttgcgttgat
    21601 tttataagca tggcgctcaa tttaaccttg aacccaacat atttacagaa tattacccgc
    21661 cgtggttagc gaaagctggc atttgtttta ctttttagcc gcataaagtc aaaattaaga
    21721 atccgttacg gctttctgaa aatcttcagc ggaccgacga gtatacctga agaaaggacg
    21781 ttagatgctt ttagctacgg cactgttaat tgttggttta cttttggtcg tttacagtgc
    21841 cgaccgcctg gtttttgccg cgtctattct ttgccgaacc tttggcatcc cgccgctgat
    21901 catcggcatg acggtggtca gtattggtac atcgttacca gaaatcatcg tctcgcttgc
    21961 cgcgtctctg cacgaacaac gcgatttagc cgttggtaca gccctcggct caaacattat
    22021 caatattttg ctgatcctcg gtctggctgc gctggttcgt ccttttaccg tccattccga
    22081 tgttctgcgt cgtgaattac ccttaatgtt gttggtcagc gtagtggccg gttccgtact
    22141 ctatgacgga caacttagtc gcagcgatgg tatctttctc ctctttctgg ctgtgctatg
    22201 gctgctgttc attgttaaac ttgcacgtca ggctgaacgt caggggactg acagcctgac
    22261 cagagagcag cttgcagagc tgccgcgtga cggcggattg cccgtcgcgt ttttatggct
    22321 cggcattgcg cttatcatca tgccagtggc cacgcggatg gtggttgata acgccacggt
    22381 gctggcgaat tactttgcca tcagcgagtt gacgatgggt ctgacggcaa ttgctatcgg
    22441 aaccagcctg ccggaactgg caaccgcaat agcgggggtt cgcaaaggtg aaaacgacat
    22501 tgctgtcgga aatatcattg gcgcaaacat ttttaatatt gtcatcgtgt tgggtttacc
    22561 cgcgctgata acgccaggag agattgagcc attggcgtac agtcgtgact acagcgtgat
    22621 gttgctggtg agcattattt ttgcgttgct gtgctggcgg cgctccccgc aaccgggccg
    22681 tggtgtaggg gtattattaa ctggcggatt tatcgtatgg ctggcgatgt tgtactggtt
    22741 atcgccaata ctcgttgaat aactggaaac gcattatgtc gcacgtagag ttacaaccgg
    22801 gttttgactt tcagcaagca ggtaaagaag tcctggcgat tgaacgtgaa tgcctggcgg
    22861 agcttgatca atacatcaat cagaatttca cgcttgcctg tgaaaagatg ttctggtgta
    22921 aagggaaagt tgtcgtcatg gggatgggga aatcggggca catcgggcgc aaaatggccg
    22981 caacgtttgc cagcaccggt acaccttcat ttttcgtcca tcctggtgaa gccgcgcatg
    23041 gtgatttagg catggtcacc ccacaggatg tggtgattgc tatctctaac tcaggtgaat
    23101 ccagcgaaat cacggcctta attccagtgc ttaagcgtct tcacgtaccg ttaatctgca
    23161 tcaccggtcg cccggagagc agcatggcgc gcgccgcaga tgtgcatctg tgtgttaaag
    23221 tagcgaaaga agcctgtccg ttagggctgg caccgaccag cagcaccacc gccacgctgg
    23281 ttatgggcga tgccctcgct gtcgcgctgt taaaagcacg cggctttact gctgaagatt
    23341 ttgcgctctc acacccaggc ggcgcactgg gtcgtaaact tctgctgcgc gtaaacgata
    23401 ttatgcatac gggcgatgag atcccgcatg ttaagaaaac ggccagtctg cgtgacgcgt
    23461 tgctggaagt tacccgcaaa aatcttggta tgactgtcat ttgcgatgac aatatgatga
    23521 ttgaaggcat ctttaccgac ggtgatttac gccgtgtctt cgatatgggc gtggatgttc
    23581 gtcagttaag tattgccgat gtgatgacgc cggggggaat acgtgtgcgc cctggcattc
    23641 tggccgttga ggcactgaac ttaatgcagt cccgccatat cacctccgtg atggttgccg
    23701 atggcgacca tttactcgga gtgttacata tgcatgattt actgcgtgca ggcgtagtgt
    23761 aaagattcaa ggataaacaa caatgagcaa agcaggtgcg tcgcttgcga cctgttacgg
    23821 ccctgtcagc gccgacgtta tagcaaaagc agagaacatt cgtctgctga tcctcgatgt
    23881 cgatggcgta ctgtcagatg gcctgattta tatgggcaat aatggcgaag agctgaaagc
    23941 gttcaatgtt cgtgacggtt atggcattcg ttgtgcgctc acctctgata ttgaagtcgc
    24001 tatcattacc gggcgaaagg ctaaactggt agaagatcgt tgtgccacat tggggatcac
    24061 tcacttgtat caggggcagt caaacaaact gatcgccttt agcgatctgc tggaaaaact
    24121 ggcgattgcc ccggaaaatg tggcttatgt cggcgatgat ctcatcgact ggccggtaat
    24181 ggaaaaagtg ggtttaagcg tcgccgtggc cgatgcgcat ccactgttga tcccgcgcgc
    24241 cgattacgtg acgcgcattg ctggcggtcg tggcgcagtg cgcgaagttt gcgacttatt
    24301 actcctggcg cagggcaaac tggatgaagc caaagggcaa tcgatatgag taaagccaga
    24361 cgttgggtta tcattgtgct atcactggcg gttctggtga tgatcggcat tgatatggcc
    24421 gaaaaagacg ataccgccca ggtggtcgtc aacaacaatg atcccaccta taaaagcgag
    24481 catacggaca cgctcgtcta taacccagaa ggggcactaa gctatcgatt gattgctcaa
    24541 cacgttgaat attattccga tcaggccgtt tcgtggttta cgcagccggt acttaccacg
    24601 tttgataagg ataaaatccc gacatggtcc gtaaaagcag ataaagccaa gctgaccaat
    24661 gaccggatgc tctatttata tggacacgtt gaagtcaacg cacttgtgcc agactctcaa
    24721 cttcgcagaa tcacgacgga taacgcgcag atcaatctgg tgacgcagga tgttacctct
    24781 gaagacctcg tcacgttata cggaacaaca tttaactcca gcggtctgaa aatgcgcggc
    24841 aacttacgca gcaagaacgc cgagctgatt gaaaaggtta gaacatccta tgaaattcaa
    24901 aacaaacaaa ctcagcctta atcttgtgct tgccagctca cttctggccg ccagcattcc
    24961 ggcatttgcc gtaaccggag acactgatca gccgatccac attgaatcgg accagcaatc
    25021 tcttgatatg caaggcaacg tggttacctt taccggtaat gtcatcgtca cccagggcac
    25081 catcaaaatt aatgccgaca aagtggtcgt tacccgtccg ggcggcgaac aaggtaaaga
    25141 agtgattgac ggctacggta aaccggcaac gttctaccag atgcaggaca acggtaaacc
    25201 cgttgaaggt cacgcttccc agatgcacta cgaactggca aaagattttg tcgttctgac
    25261 gggtaatgct tatctgcagc aggtcgatag caacattaag ggcgataaga tcacttacct
    25321 ggtaaaagag cagaaaatgc aggctttcag cgacaaaggc aagcgcgtaa ccaccgttct
    25381 ggtgccgtcg cagctgcagg acaaaaacaa caaaggccag accccggcac agaagaaggg
    25441 taattaattc gttatggcaa cattaactgc aaagaacctt gcaaaagcct ataaaggccg
    25501 tcgcgtggta gaagacgtca gcctgaccgt caactccggg gaaattgtcg gtctgctggg
    25561 gccaaacggt gccggtaaga ccaccacttt ctacatggtt gtaggcattg tgccgcgcga
    25621 tgcgggcaac atcattattg atgatgacga tatcagtctg ctgcctctgc atgcacgcgc
    25681 gcgccgcggt atcggctatc tgccacagga agcctccatt ttccgtcgcc tcagcgttta
    25741 cgataacctg atggcggtac tgcaaattcg tgacgacttg tctgctgaac aacgtgaaga
    25801 ccgcgcgaac gagctgatgg aagagtttca cattgagcac ctgcgtgaca gcatggggca
    25861 gtcactctcc gggggtgaac gtcgccgtgt agaaattgcc cgcgcactgg ctgcgaatcc
    25921 gaaatttatt ctgctcgacg aaccgtttgc cggggttgac ccgatctcgg ttatcgacat
    25981 taaacgcatc attgagcacc tgcgcgacag cggcctgggc gtgctgatca ctgaccacaa
    26041 cgtgcgtgaa acactggcgg tttgtgaacg cgcttatatc gtcagtcagg ggcatttgat
    26101 cgcccacggc acgcctacag aaatcttaca agacgaacac gttaagcgtg tataccttgg
    26161 ggaagacttc agactctgat agggtagaag tttgcgacgt tttagcagga gagtacgatt
    26221 ctgaacatga agcaaggttt gcaactcagg cttagccaac aactggcgat gacgccacag
    26281 ctccaacagg caattcgtct gttgcagttg tcgacgctgg aacttcagca ggagctacag
    26341 caggcgctgg agagtaatcc gctgcttgag caaatcgaca ctcatgaaga aatcgacacc
    26401 cgcgaaacgc aagacagtga aacgctggac accgccgacg cgctcgaaca aaaagagatg
    26461 ccggaagagc tgccgctcga tgccagttgg gacaccattt acaccgctgg tacaccatcc
    26521 ggcaccagcg gtgactacat tgacgatgag ctgccggtct atcagggcga aacgacgcag
    26581 accttgcagg attacctgat gtggcaggtc gagctgacac cgttttccga cactgaccgc
    26641 gctattgcta cctctatcgt cgatgccgtt gatgacatcg gttatctgac tgtcccgctg
    26701 gaagatattc tcgaaagtat gggcgatgaa gagatcgaca tcgacgaggt tgaagccgtc
    26761 cttaagcgga tccaacggtt tgatccggtc ggtgtagcgg caaaagatct gcgtgactgc
    26821 ctgctaatcc aactctccca attcgataaa accacgccgt ggctggaaga ggccagactg
    26881 atcattagcg atcatctcga tctgttagcc aatcacgact tccgcacttt aatgcgcgtc
    26941 acgcgtctga aagaagatgt gctgaaagaa gccgtcaatc tgatccagtc gctcgatccg
    27001 cgccccgggc agtcgatcca gactggcgaa cctgagtatg tcattccaga tgtgctggtg
    27061 cgtaagcata acggtcactg gacggtagaa ctcaacagtg acagcattcc gcgtctgcaa
    27121 atcaaccagc actacgcctc gatgtgcaat aacgcgcgca acgatggtga cagccagttt
    27181 atccgcagca atctgcagga tgccaaatgg ttgatcaaga gtctggaaag ccgtaacgat
    27241 acgctactgc gcgtgagtcg ctgtatcgtt gaacagcagc aagccttctt tgagcaaggt
    27301 gaagaatata tgaaaccgat ggtactggcc gatatcgccc aggctgtcga aatgcatgaa
    27361 tcgacgatat ctcgcgtgac cacgcaaaaa tacctgcata gtccacgagg catttttgaa
    27421 ctgaagtatt tcttttccag tcacatcaat accgagggcg gcggcgaagc ttcctccacg
    27481 gcgattcgtg cgctggtgaa gaaattaatc gcggcggaaa acccagcgaa accgttgagc
    27541 gacagcaagt taacctcttt gctgtcggaa caaggtatca tggtggcacg ccgcactgtt
    27601 gcgaagtacc gagagtcttt atccattccg ccgtcaaacc agcgtaaaca gctcgtttga
    27661 cccaaccgat aaggaagaca ctatgcagct caacattacc ggaaataacg tcgagatcac
    27721 cgaggcactg cgcgaatttg ttacagccaa atttgccaaa cttgagcaat attttgaccg
    27781 aatcaaccag gtctatgttg ttctgaaagt ggagaaagtc acccacacct cagatgcaac
    27841 actgcatgta aacggtggtg aaattcatgc cagcgcggaa ggtcaggaca tgtacgctgc
    27901 cattgatggc ttaattgata agctggcacg ccagttgacc aaacataaag ataaactgaa
    27961 acaacactaa ttgtccgggc aattagcatg tgcatggcgg cctgttgtgc ggcacaacgg
    28021 gccatttgta cggttaatgc tccgagcctg ttccactgtt tgagtgggca ggttcttagg
    28081 tgaaattatg acaaataatg atacaactct acagcttagc agtgttctta acagggaatg
    28141 tacgcgaagc cgcgtccact gtcagagcaa aaaacgcgcc ctggaaatca tcagcgagct
    28201 ggcggcgaaa caacttagcc tgccgcctca ggtggttttt gaagctatcc tgacgcggga
    28261 aaaaatgggc agtaccggta tcggcaatgg tattgccatt ccgcatggca aactggaaga
    28321 agatactctg cgcgccgttg gcgttttcgt tcagctcgaa acgcctatag ctttcgatgc
    28381 catcgacaac caaccggtgg acttactttt tgccctgctg gtgtcggcag accaaactaa
    28441 aacgcacctg catactctgt cgctggtggc gaaacgtctg gcggacaaaa ccatctgccg
    28501 ccgtttacgc gcagcccaga gcgatgaaga gctgtatcaa atcattacgg ataccgaagg
    28561 tactccggat gaagcgtagt tattcggtaa tgtctctttt agacgttgtg aggagaaaca
    28621 gtacatggta ctgatgatcg tcagcggacg ttcaggttca ggtaaatctg tcgccctgcg
    28681 tgcgctggaa gatatgggtt tttactgcgt ggataacctt cccgtagtgt tgttacccga
    28741 tctggctcga accctggccg atcgcgagat ttctgccgcc gtcagcattg atgttcgtaa
    28801 tatgccggag tcaccagaaa tattcgaaca ggcgatgagt aacctgcctg acgctttctc
    28861 accgcaactc ctgttcctgg atgccgaccg taatacctta attcgtcgtt acagtgacac
    28921 gcgccgactg catccgcttt ccagcaaaaa cctgtcgctg gaaagtgcta tcgacaaaga
    28981 aagcgatttg ctggagcctc tgcgttcgcg agcggatctg attgtcgata cctcagaaat
    29041 gtccgttcac gagctggctg aaatgctgcg tacccgtctg ctgggtaaac gtgaacgcga
    29101 actgaccatg gtctttgagt ctttcggctt caaacacggt atccctatcg atgcagatta
    29161 cgtctttgac gtgcgcttct tgccgaaccc gcactgggat ccgaaactgc gtccaatgac
    29221 aggtcttgat aaacctgtcg ccgcgttcct cgaccgccac acagaagtac acaattttat
    29281 ctaccagacg cgaagctatc ttgagctatg gttacctatg ctggaaacca acaaccgtag
    29341 ctacttgacg gtcgccattg gttgtaccgg cgggaagcac cgttcggtgt atattgcaga
    29401 gcaactggca gactacttcc gctcgcgcgg taaaaacgtc cagtcacgcc atcgtacgct
    29461 ggaaaaacgt aaaccatgac cgtcaagcaa actgttgaaa tcacaaacaa gctgggcatg
    29521 catgcccggc ctgcaatgaa gctgtttgaa ttaatgcagg gttttgacgc tgaagtgctg
    29581 ttacgtaatg acgaaggcac cgaggccgaa gccaacagcg ttattgcgct gctgatgttg
    29641 gattctgcca aaggacggca gattgaagtt gaagcgaccg gcccacagga agaggaagca
    29701 ctggccgccg ttatcgccct ctttaactct ggttttgatg aagattaatc ttcatcactt
    29761 tgacatacaa ctcccttcaa actccccccg acaataagaa aatcacgtac tgaaatcgtt
    29821 ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa ccttaagaaa
    29881 accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg agatcgtcag
    29941 aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct gggcaccgga
    30001 agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa gattgtctac
    30061 catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta cgcgcatctt
    30121 ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt tgagacggat
    30181 tgcggaaccg gttatgtcta cgatgtgatt gcagattttg acggcaaact ctccattacg
    30241 ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact gcgccagcta
    30301 ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc gctgaagccg
    30361 cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt ctgcgacaac
    30421 attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg tctgcgtaag
    30481 caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc cctgcagaaa
    30541 gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc ttaatccagc
    30601 tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa aatccacgcc
    30661 tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg attaggtaat
    30721 acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt gtggaaataa
    30781 cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat attcaggtaa
    30841 accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa tccagcttcc
    30901 agcccttttc gtacccagct acgcccatcc cataaaaaga gatttttggc cgtctgctga
    30961 gaaatcgttg aagcaccgcg aatacggttt tcattgcgct cgttgtgcgc cagggctttc
    31021 tcaatggaag cgacatcaaa gccccagtgc tcaggaaatt tctgatcttc tgcggcaatc
    31081 accgccagtc ccatccacgg cgagatttga tccatactga cccagtcaga atgtgccacg
    31141 taacgaaaat tgccatgcag ccaggcgctg acctgtcgct cgaccattac cgctgagaag
    31201 ggaacaggcg caacactaaa caacgcgatg cccccgcccc agaaaacggc gaggacaacc
    31261 attaacagca aaagaaaacg gcgaacgaaa ctaaacaccg ttaagcggct tttactcatt
    31321 cagccagaac cagcacgcgg gaaaccagct tatcaatgcc gctcgccgct tctgcaatgt
    31381 tctgcgccag catatatgct ggggtggtga caatcttatt gtcttcatca accacgatat
    31441 catcgacagg acagggcaca tgctccgcgc ccatctcttc cagcacttct gcggtatcga
    31501 tatcagtacc gatggtcaaa cgcagcggga aatcgaaaat tttcggcagc atcgccgggg
    31561 caatacacat aaaaccaagc ggttttccgg cctgatgcat cgcttgtgcc agcgccttta
    31621 attcacggtc aacggtgcat tcgctaccaa gagtggcaaa attgcttaaa ttcttcgccg
    31681 cgccaaaccc ccccggcaca atcaacgcat ccagttcagc ggcatcggcc tgggccagag
    31741 gacggatttc accgcgcgtt attcgtgccg cttcaatcag cacgttgcgc gtttccgtca
    31801 tcgcttcgcc agttaaatgg ttgataacat caacctgctg cttatccggt gcaaagcaga
    31861 ccgcctgcgc accgctgcgt gaaatagcta acaacgtcaa caccgcttca tgaatttcag
    31921 aaccgtcata gacgccgcat ccgctcagaa ttacgccaat tttcttcatt gtgatcatcc
    31981 tttcgcaatt gactgaaaca cattaatttt tttaataaaa atggtacgca tcacacattt
    32041 aactgattca tgtaacaaat catttaagtt ttgctatctt aactgcgtgc ggcctgaaaa
    32101 acagtgctgt gcccttgtaa ctcatcataa taatttacgg cgcagccaag atttccctgg
    32161 tgttggcgca gtattcgcgc accccggtct agccggggtc attttttagt ggcttttgcc
    32221 acccacgctt tcagcacttc tacgtcgtga cgccactctt ctttcatctc ttcaatccat
    32281 tcaccgacgt tatcttccca ggcaggaagg tcaggagact gaatttgctg acccagctgt
    32341 tgcagatggc gtaaccccac tgaccccgcc gcacctttaa ttttatgtcc ttcctcaaca
    32401 atgccttttt tatcctgcgc cgtcagattc gactccagca cgctgacata gcccggcatc
    32461 atcttctcaa acaccgctaa cccgtcggtg atcagcttcg gtcctacaag ttcgagatac
    32521 tgttccagca tgggaatatc gagcaatgct tctgatttac tgttctcttc tgtcgtcacc
    32581 gtactctcct catcatcctg ggtatcccag aatttcttga tcatcgcggt tagcgccgga
    32641 acagaaagcg gcttactcag cacatcatcc attccagcat tgaggtactc ttgtttgtct
    32701 ttcagcacgt tagcggttaa ggccaccagc ggcggtaaat cctctcgcgg ataacgcttc
    32761 gtcagttcac gagagatatc cagcccggtc atatctggca actgaatatc cagtaacacc
    32821 aggtcgtatt cgcccggttt aaacatctct agcgccgcct tgccggtcat ggcgacatca
    32881 acgctgttac ctaatttttc cagcacagaa cgcgcaacaa tcacgttcag ttcaatgtct
    32941 tccaccagca gcacattcag cgccggtaaa ggcatatcgt cttcatcaaa cgcatcatcg
    33001 acctcttctg ctaccgacgg tgcgtggatc gtcaacgtaa aggttgaacc tttgccctgt
    33061 tcgctggtaa ccgtaatatc gccgcccata tttttcgcca gacgacgaga aacggccaga
    33121 ccaataccgg tgccggtggc aggtttaccg ccatgactgt ctttcacctg gtaatacatg
    33181 gcgaaaattt tatccagctc atcctgcgga atgccgatac cagagtcttc cacttcaaaa
    33241 tgcagcatat cgccttcatc gtagcgcacg cgcacggtaa cctggccttg ctgggtgaat
    33301 ttgacggcgt tactgatgag gttccacagg atctgccgta aacgcgtccc gtcggtaatg
    33361 acctgatgcg gtaatggcag cgtcggctcc aggttaaagc gcaatccttt ttgttgcgcc
    33421 tgcaatgcgg agagattttc cagatcggca aggaagctgg tgaaatcaac cggttgatta
    33481 tcaagctgga ccttgcgccg ttccatctta tccatgtcga taatatcgtt aaagatattc
    33541 cccagcgtga cggccgaaac atggatggtc ttgagatatt tttcctgctc ggcggtgagt
    33601 tcggtatcca gcagaatgcg gctcagaccg acgataccgt tcagcggtgt acgcaattcg
    33661 tgactgatgg tggagataaa cgtcgttttg tcgcggctgg cccgttcaag cgcatcctga
    33721 taccgcttac gctcggtaat gtctcgacca aagcccatca aaccgtgacg tttacccacg
    33781 cggtcgtagt acggcacttt acggatttca aagcaggctt tgcgcccgtc cgggtaatcc
    33841 agccactgtt cataggtcag tgacacatta tgacggaaca ctttttcatc ggtttcaatg
    33901 acttttgcgg cggcttccgg tgagtaaaca tcagcaggtt tcaggtgaac cagttgtttt
    33961 tcgctttttc cggtcagcag ctccatcgcg cggttacagc cggaaaactc tttatcttcg
    34021 ttacgataaa aaaccaggtc gggtgaagca tcaaggaagg aacgtaagaa tgaggattgc
    34081 tgctcgagct gaatttgtgt ctcttcgcgc tctttgattt caattttcag ttggccgaag
    34141 gtttcctgca gttctgcttc cgctttttca cgaacggcga tttcctgatt tagctgggca
    34201 atattatctt ttaactgaac gttgaggctc aaatcgcgct cgcgcatctc ctccagtttt
    34261 tgcaccagcc gtgacagacg ttgtcgtgac tcctccagtt gctcgacgac caccgataga
    34321 aagtagaccg cccacggcgt aatcaacaaa ccaaagaaga tagaacgaat aacatcaatg
    34381 ctttcgacct gaccatgcag caccatggtt accgccattt gtaccacaat ggcaagaacg
    34441 acgagggcca gcgccagcaa cattgagaag cgcaccagac ctaacttcat catcaggtca
    34501 acataatact gcgccagcag acgaatttgc ttcattaggg aattccttca cgacaacctg
    34561 ccccaataat acccaattct gacagttacg ttataaattg tgtgagaaat gcggaattcc
    34621 cctcaacgac ctactccgtt ggaggtagcc acggacgtcc cagcgccgat ccctgtacgc
    34681 catgttcatt cagatagcga tccagctcga ccattcccgt ccatcgattt tcgcaccaca
    34741 gaggagcaag cagcgtcggg cgacgggcgc tggcggaaat gcggtggtag atcacttccg
    34801 gtggcgtatg gcgaatcatt tctccggcag tgagcgtgta atcctccagt tcaataccgt
    34861 tcaaacgccc cgcttcccag gctttcgcca taatgctgcc tttcacaata tgcaacggat
    34921 gtagctttat accgtcaaca ccggtttcca ctacccgctc cagcgtttgc agacattcag
    34981 ccagcccttc gcctggcaga ccgacaatta aatgcgagca caccttcagc ccgcgctggc
    35041 gtgccagctg cgtcgtgcgc tgataacagg cgaaatcatg accgcgatta atgcgatgta
    35101 gcgttttgtc gtgcacggtt tgcagcccca gctccagcca cacttcgtag ccctggtctt
    35161 tatattcgca aagcagatcc agcaccgcat ccggcacgca gtccgggcgc gtgccgacac
    35221 acaaaccgac aatattggcc tggctcaccg cctgttgata catcgaacgc agcacctgaa
    35281 cttcggcaaa ggtgctggta tacgcctgga aataggcgag gtaacgtttc gcgcgattaa
    35341 ttaaattcgc ctgatgcgcc agctgttcgg caatggaacg atgctgctgc gcttcgtcgg
    35401 caaacgaggc aacattacag aaggtgcagc cgccgcgccc gatggtgccg tcgcgattcg
    35461 ggcaactgaa accgccgtgc agcgtcagct tatgcacctt ttgcccataa cgacgggtga
    35521 gatcaccacc aaacatattg actaatttct gtaactgcat aatctgatag accgcgcctt
    35581 gaaaagaggc caaagcctgc catttttagc tcaattcggc gatgacctgg atcaatcgtc
    35641 ccggcccgct tttatcaact gcataatcaa tcaaaattac cgaaatttca tgcataatca
    35701 cataaatcac ttttgcttat cttgtgtcag atttttttat ctccttatgg attttaggca
    35761 aaaacagtag catgaaacgt cattaccaat taatgcagta taaaatgctg gttttctcgt
    35821 caattcaagg caggataagg gttaacacgc ctttatgaca gtcaggaatt gactgtttct
    35881 ctaacgactt cccttttagc cttaaagata aaatctattt taatttcagc catttaataa
    35941 agaattttgc gctaaagcac atttctgtac caataagctt gccatttgac ctgtatcagc
    36001 tttcccgata agttggaaat ccgctggaag ctttctggat gagcagcctg ctcatcatat
    36061 ttatgcagta attgagatcc cctcttcacc gtattaaccg atgcgaaaag gacaacaagg
    36121 gggcgaatgc gaggcgcgcg tatgacacgc aacccccgtc gccacgctct ttctgtaccc
    36181 gtgcgcagcg gttcggtagt ggggttcccg cagagcctgg gggaggttca cgatatgttg
    36241 tacgataaat cccttgagag ggataactgt ggtttcggcc tgatcgccca catagaaggc
    36301 gaacctagcc acaaggtagt gcgtactgca atacacgcac tggcccgcat gcagcaccgt
    36361 ggcgcgattc tcgccgatgg taaaaccggt gacggttgcg gcttgctgtt acaaaaaccg
    36421 gatcgctttt ttcgcatcgt tgcgcaggag cgcggctggc gtttagcgaa aaactacgcc
    36481 gtcgggatgc tattcctgaa taaagatcct gaactcgccg ctgccgcacg ccgcatcgtt
    36541 gaagaagaac tgcaacgcga aaccttgtcg attgtgggct ggcgtgatgt ccccactaac
    36601 gaaggcgtac tgggtgaaat cgccctctcc tctctgcccc gcattgagca aatttttgtg
    36661 aacgccccgg caggctggcg tccgcgcgat atggagcgcc gtctgtttat cgcccgccgc
    36721 cgcattgaaa agcgtctcga agccgacaaa gacttctacg tctgtagcct atcgaatctg
    36781 gtgaacatct ataaaggtct gtgtatgccg gcggatctgc cgcgctttta tctggatctt
    36841 gcggacctgc gtctggaatc ggccatttgc ctgttccacc agcgcttctc cactaacacc
    36901 gtaccgcgct ggccgctggc gcaaccgttc cgctatctgg cgcataacgg tgaaatcaac
    36961 accatcactg gtaaccgcca atgggcgcgt gcgcgtacct ataaattcca gacgccgctt
    37021 atccctgatc tgcacgacgc cgcaccgttc gtcaacgaaa ccggctctga ctccagttcg
    37081 atggataaca tgctggaact cctgctggca ggcgggatgg atatcatccg cgccatgcgt
    37141 ctgttagtgc cacccgcctg gcagaacaac ccggatatgg acccggaact gcgtgccttc
    37201 tttgacttta actccatgca tatggagccg tgggatggcc cggcgggcat cgtgatgtcc
    37261 gacggtcgtt ttgccgcctg taacctcgac cgtaacggtc tgcgtccggc gcgctacgtc
    37321 atcaccaaag ataagctcat cacctgcgcc tctgaagtcg gtatctggga ttaccagcct
    37381 gacgaagtgg tcgaaaaagg ccgcgtcggg ccaggcgaac tgatggttat cgacacccgc
    37441 agtgggcata ttctgcactc ggcagaaacc gatgacgatc tgaaaagccg ccatccatat
    37501 aaagagtgga tggagaaaaa cgtccgccga ctggtaccgt ttgaagatct gcccgatgaa
    37561 gaagtgggta gccgcgaact ggacgacgac acgcttgcca gctaccagaa acagtttaac
    37621 tacagcgcgg aagagctgga ctccgtaatt cgcgtactgg gcgaaaacgg tcaggaagcg
    37681 gtcggttcga tgggcgatga taccccattc gccgtgctct ccagtcagcc gcgcattatt
    37741 tacgactact tccgccagca gtttgcccag gtgactaacc cgccaatcga cccgctgcgt
    37801 gaagcgcatg ttatgtcgct cgccaccagt atcggtcgtg aaatgaacgt cttttgcgaa
    37861 gcagagggcc aggcgcaccg tttaagcttt aaatcgccga ttctgctcta ctccgatttc
    37921 aaacagctca cgacgatgaa agaggagcac taccgcgcag atacgctgga tatcaccttt
    37981 gacgtcacta aaaccacgct cgaagcgaca gtcaaagagc tgtgcgacaa agccgaaaaa
    38041 atggtacgta gcggcaccgt gctgctggtg ctctccgacc ggaatatcgc taaagatcgc
    38101 ctgccggttc cagccccgat ggcggttggc gcgatccaga cccgtctggt cgatcaaagc
    38161 ctgcgttgcg atgccaacat catcgtcgaa accgccagcg cccgcgatcc gcaccacttc
    38221 gccgtgttgc tgggcttcgg cgcgacggct atttatccat accttgccta tgaaacgctg
    38281 ggccgcctgg tagacaccca tgcgattgcc aaagattatc gtaccgtgat gctcaactac
    38341 cgtaacggca tcaacaaagg cttgtacaaa atcatgtcca aaatgggcat ctccaccatc
    38401 gcctcttacc gctgctcgaa actgtttgaa gcggtcggtc tacacgatga tgtagtgggc
    38461 ctgtgcttcc agggggcggt cagccgcatt ggtggggcaa gctttgaaga cttccagcag
    38521 gatctgctga atctgtcgaa acgtgcctgg ctggcgcgta agcccatcag ccagggcggc
    38581 ctgctgaaat acgtccacgg cggcgaatac cacgcctaca acccggacgt ggtgcgcacg
    38641 ctgcaacaag cggtacaaag cggcgagtac agcgactatc aggaatacgc gaagctggtt
    38701 aatgagcgtc cggcaaccac gctgcgcgat ctgctggcaa ttacgccggg tgaaaacgcg
    38761 gtcaacattg ctgatgttga accggcaagc gaactgttta aacgcttcga taccgctgcg
    38821 atgtctatcg gcgcgttaag cccggaagcc cacgaggcgc tggcggaagc gatgaacagc
    38881 atcggcggta actcaaactc cggtgaaggc ggtgaagatc cggcgcgcta cggcaccaac
    38941 aaagtgtcgc gcatcaagca ggtggcttcc ggtcgctttg gggttactcc ggcgtatctg
    39001 gtcaatgccg acgtcattca gattaaagtc gcccagggcg cgaagccggg cgaaggcggt
    39061 cagttgccgg gtgataaagt cactccttac atcgccaaac tgcgctattc ggtgcccgga
    39121 gtgacgctga tctccccgcc gccgcaccac gatatctact ctatcgagga cttagcgcag
    39181 ctcattttcg acctcaagca ggttaacccg aaagcgatga tctccgtgaa gctggtttcc
    39241 gaaccgggcg taggcaccat cgcgactggc gtggcaaaag cttatgccga tttaatcacc
    39301 atcgcaggct atgacggtgg caccggcgca agcccgctgt cgtcagtgaa atacgcaggt
    39361 tgcccgtggg agctggggct tgttgaaacc cagcaggcgc tggttgccaa tggtctgcgt
    39421 cataagatcc gtttgcaggt cgatggcggc ctgaaaaccg gtgttgatat catcaaagcg
    39481 gcgattctcg gcgcagaaag cttcggcttc ggcactggcc caatggtcgc gctcggctgt
    39541 aaatatctgc gtatttgcca cctgaacaac tgcgcaacgg gtgtggcaac tcaggatgac
    39601 aaactgcgta agaatcacta tcacggcctg ccgttcaagg tgacgaatta ctttgagttt
    39661 atcgcccgtg aaacccgcga gctgatggca cagctggggg taacgcggct ggtggatctg
    39721 attggtcgca ccgacctgct gaaagagctg gacggtttca ccgccaaaca gcagaaactg
    39781 gcgctgtcga agctgctgga gaccgccgaa ccgcatccag gtaaggcact ctactgcacc
    39841 gaaaacaacc cgccgtttga taacggcctg ctgaacgcgc agttgctgca acaggcgaaa
    39901 ccgtttgtcg atgaacgcca gagcaaaacc ttctggttcg atatccgcaa taccgatcgc
    39961 tccgtgggcg cgtcgctttc aggctatatc gcccagacgc acggcgatca ggggctggca
    40021 gccgatccta tcaaagcgta cttcaacggc accgcaggcc agagtttcgg tgtatggaac
    40081 gcgggcggcg tggaactgta cctgaccggg gatgccaacg actatgtcgg taaaggcatg
    40141 gcgggcggct taatcgccat tcgtcctccg gttggttccg ccttccgcag ccatgaagca
    40201 agtattatcg gcaacacctg cctgtatggc gcgaccggtg gtcgtctgta tgccgcaggc
    40261 cgcgcgggtg aacgtttcgg cgtgcggaac tccggtgcta tcaccgtggt agaaggcatt
    40321 ggcgacaacg gctgtgaata tatgacgggc ggtattgtct gtattctggg taaaaccggc
    40381 gttaacttcg gtgcgggcat gaccggcggc ttcgcttacg ttctcgatga aagcggtgat
    40441 ttccgcaaac gcgttaaccc ggaactggtc gaggtcttaa gcgttgacga tctggcgatc
    40501 catgaagagc atctgcgcgg tcttatcacc gagcatgttc agcatactgg ctctcagcgc
    40561 ggtgaagaga ttctggcgaa ctggtcaacc ttcgccacta aatttgcgct ggttaaaccg
    40621 aagtccagtg atgtaaaagc actgctgggt catcgtagtc gtagcgcagc agagttgcgc
    40681 gtgcaggcgc agtaaggggt agcaacaatg agtcagaatg tttatcaatt tatcgacctg
    40741 cagcgcgttg atccgccaaa gaaaccgctg aagatccgca aaattgagtt tgttgaaatt
    40801 tacgagccgt tttccgaagg ccaggccaaa gcgcaggctg accgctgcct gtcgtgcggc
    40861 aacccatact gcgagtggaa atgtccggta cacaactaca tcccgaactg gctgaagctc
    40921 gccaacgagg ggcgtatttt tgaagcggcg gaactgtcgc accagaccaa caccctgccg
    40981 gaagtttgcg gacgagtctg cccgcaagac cgtctgtgcg aaggttcctg cactctgaac
    41041 gatgagtttg gcgcggtgac catcggcaac attgagcgtt atatcaatga taaagcgttc
    41101 gaaatgggct ggcgtccgga tatgtccggc gtgaagcaaa ccggtaaaaa agtggcgatt
    41161 atcggcgcag gcccggcagg tctggcgtgt gcggatgtcc tgacgcgtaa cggcgtaaaa
    41221 gccgttgtct tcgaccgtca tccagaaatt ggcgggctgc tgaccttcgg tattccggcc
    41281 ttcaagctgg aaaaagaggt aatgacgcgt cgccgtgaaa tcttcaccgg catgggtatt
    41341 gaattcaaac tcaataccga agtgggccgc gacgtgcagc tggacgatct gctgagtgat
    41401 tacgatgccg tcttccttgg cgtcgggact tatcagtcaa tgcgcggcgg gctggaaaac
    41461 gaagacgccg atggcgtgta cgcagcgctg ccgttcctta tcgccaacac caaacagtta
    41521 atgggctttg gtgaaacccg cgacgaaccg ttcgtcagca tggaaggcaa acgcgtggtg
    41581 gtccttggcg gtggcgacac tgcgatggac tgcgtgcgta cgtccgtgcg ccagggagcg
    41641 aagcacgtta cctgtgccta tcgtcgtgat gaagagaaca tgccgggttc ccgccgcgaa
    41701 gtgaaaaacg cgcgggaaga aggcgtagag ttcaaattca acgtccagcc actggggatt
    41761 gaagtgaacg gtaacggcaa agtcagcggc gtaaaaatgg tacgtaccga aatgggcgaa
    41821 ccggatgcca aaggccgtcg ccgcgcggag atcgttgcag gttccgaaca tatcgttccg
    41881 gcagatgcag tgatcatggc gtttggtttc cgtccacaca acatggaatg gctggcaaaa
    41941 cacagcgtcg agctggattc acaaggccgc atcatcgccc cggaaggcag cgacaacgcc
    42001 ttccagacca gcaacccgaa aatctttgct ggcggcgata tcgtccgtgg ttccgatctg
    42061 gtggtaaccg ctattgccga aggtcgtaag gcggcagacg gtattatgaa ctggctggaa
    42121 gtttaagctc tgtcccagcg ccgcctcctc agaaaagagt cggcgctgaa attctccttt
    42181 ctttctggaa agcgcctcgc ataatctgaa gaaaaccaat gtaatacgct catctggatt
    42241 gttaccggta gatgcccggc gtatagctga ttccattatt caacgcagcg ccatataaag
    42301 ggacagataa tggaatcgct ctcagagaga acctcaacag gctaccagca aatccacgac
    42361 ggtattattc acctggtcga tagcgcccgg acggaaacgg tacgtagcgt taacgcgctg
    42421 atgaccgcga catactggga aattggccga cgaattgtcg aatttgaaca aggtggcgag
    42481 gcaagggctg cgtatggtgc gcagctaatc aagcgactat caaaggattt aagtctaagg
    42541 tataagcgtg gtttctctgc aaaaaactta cgccaaatga ggctttttta cctctttttt
    42601 caacatgttg aaattcgcca gacagtgtct ggcgaattaa caccattggg aattccgcag
    42661 acaccatctg cagaattccc atcggcaaag atttggcaga cgctgtctgc caaattgaga
    42721 ggctgtttga tatttccaca gcttttgtat tgcatctcct attttcggtt tgtatattca
    42781 tgcaaataaa cgaaggaaag ttgccgtgat ttacattgaa cagcttgaat taatccataa
    42841 aagtggtgat gtgttgtatc cagtaaaaat aaccagaaaa tcatcaggaa aaactgcctt
    42901 ccaccttgtt ccattcggct taaataagac tcacgacctt ctagaggtgg aagatgcttc
    42961 agaagcaatt cggctggtga ttgatgaacg tcattccatt cgctgcagta cacttacagc
    43021 aactattacg aataaaaagg gcaagcgaat taagcggaca ggtatataca gtatcaaggg
    43081 tgttaacata aaagaataca acgtcaggta atggcacaga gcggtccgtc agttcaggtt
    43141 tggctctgtg ccaaagatgg gtcaggtcac acctgatcta ataaatttaa gccattaaat
    43201 cagccttaac cttcaccacc acctttttaa tctcacccgg ctcgccaaca acgcatcccg
    43261 gcttatgcgg ctctcctagc ataaacacgg cgaacattcc cggtttcagg atgatggctt
    43321 gctcgttttc aatggtgctg caaagctggt aatcatcctc atggtggaac tcttcacact
    43381 gacgcgcagt gcctgccatg ccaaacagaa tccgttcttc accgtttaat aacagctgga
    43441 tatcaatgta ttgctcgtgc agctccgctt ttttctcaac gggcgattga gtgttaaacg
    43501 tcatgacatt cataaaaata ttgtcgccct gtaattcgta acgacccggc gctttttctt
    43561 gcggtctggc agctaatgcc agcgttaacg cgtcctgtaa cgcaggatgt aacccagcag
    43621 acggtaatga ccgtacttca cccatcatca taatttttct ccctgggcca acagcgctgc
    43681 cccaagtaaa cctgcatcat ggcggtagtg cgccgccagt aaatcaacat gaaataccgc
    43741 tggctcctgc gccagatacg tttccaccaa cgccagatac ccttctgcca gaccaacgct
    43801 gccaccgacc accacgcact ggcaatcagt tgtggcttta atatcagcga tcagccttgc
    43861 cagcacatgt gcggagcggt gaatcagttg ctgcgcctgc tcgtcgccct gcccggcgcg
    43921 tgtgaaaata gttctcgcat ccgcgcccat gagttcacct tgcgctgccg ctgcaatacc
    43981 gcgaccagaa gcaatcgctt ccacgcaacc tgtgcgtcca cagccgcaga ctgggccgtg
    44041 tggatcggca agcgtatgcc cgatatgccc cgccagaccg ccagggccgg taagcagttt
    44101 accgccgctc actacaccgc cgccgacgcc agtggaaacg gtgataaaca ccatctcggt
    44161 tacatcgcct tccagcgcct gatactctgc ccatgctgcg gcctgcgcgt cgttaatggc
    44221 aatggtcggc aaattggtaa gttgttccag cgttttgact aacggaaagt gtagcaatcc
    44281 accgagatta tgcggattca gcgccagcaa actgccgtcg cgaataattc cggttgaagc
    44341 gatggcaacc cgctgcgcat gagcttgcaa cggagagact aatgcggata aggcatcacg
    44401 caaggcttct ggtgtctggc tggctggcgt aggaagttca cgacgatcgc ggatctgccc
    44461 gtcagcgcca atcagcgcgg cggcaagttt agtaccgccg atatcaatcg ccagtgtggt
    44521 catagcaccg cctttttcat cgctgtgttg taccactgac aaatgtgttc aagacgcgta
    44581 atggcagaac cgaccgtcac cgcccacgcg ccgtggcgca tcgcatctgc cgcctgagca
    44641 ggcgtgttgt aacgcccttc ggcaatcacc cgacatccgg cttcgctcaa cgttttcacc
    44701 agcgccagat ccggctcttc tggcgtttca ggcgtggtat agccagaaag cgtagtgcca
    44761 ataatttcgg cgcccagctt ttggcatgcc aggccgtctt ccggcgttga gcagtcggtc
    44821 atcgccagta aaccgtgatg atgtatacgt gccagcagcg tttcaacagg caccggacgc
    44881 gggcggtcgg tgccgtcaat ggcgataatg tccgcgcccg cctgcgccag cgcatcgaca
    44941 tcttcaatat aggccgtgat gcgtaccgga gaatcctcca gatcgcgttt cacaattcca
    45001 ataatcggca cgctcaccac cgcacgcgtg gcttgcagat ttgccacacc ttcgatgcga
    45061 atggcaaccg cgcccgcctg ctctgccgct aatgccatgg ctgcgacgat ttcgggttta
    45121 tcgagcgggc tgtcaggaat cggctggcag gagacaatca ggccaccgtt agcagcgatt
    45181 ttttgatcca gttgtgcaag taacgacata catcttccct tagcgaaagg cccggtacat
    45241 agaccgggca acaggattaa cttttggttt tgactaaatc gtttttggcg ctgccaaatg
    45301 gcacggcacc gctaaatggt ttgccgtcga tagcgtcatg agtacgcaat gcttccgggc
    45361 gcagccaacg ctgaacgcgg gaaggcatat ccagaccaat cagcaggatc accacaaatg
    45421 tcaggctgaa cgagagcgat gccagcgcag tacccagatc cagacgttga gcgatcaacg
    45481 cgccgatgat tggggccagt gcgccaccca atgcgccaac gttgtaggta aagcccaggc
    45541 ccgctgcacg ctggtcggta tcgaaataac cgccaatcag tttcggtaag atcccggcga
    45601 tcccttgtcc aagcatttgc tggaagaaca gtaacagacc gagcacccag acgtttgcgc
    45661 cgccaatcgc aaataccgga ataatcagca gctgcgaggc cagcaggcta caaacgtacg
    45721 ctttgcgggt tcccagccag tcaccgagga agcctcctac gcagcatccc acggccgcgc
    45781 caaagccact aaagaacagc acattggcga cagtatgcgg gttataagcc agatcggttt
    45841 tcagatacgt tggcagcagc gcctgaatcg gccatgagta gaggaaagca aacaacacga
    45901 ccaccatcag cattacgccc gttggccagc gtttgcctgt actctgcacc ataaagctga
    45961 taaagattgc ggcgcataac agcccaagaa cagcgacgat cgcggcattt tgcaggttac
    46021 cggcgaagca gaaccacagc gcagtagccg ccgccagtgt cattacgata ttggcaatgc
    46081 gatgttcacc acggtagaga atatccacca ttgtgcgtac tggtgcttta cctgcgtgtt
    46141 tctctttcca gtcttccgct tccgggatgt ttttacgcag ccagagagca aagatgattg
    46201 gcaaaatgcc gataaagaac agcgcacgcc aaccccagac cggaaccacc aggctataga
    46261 cctgagcggc aacgacggcc cccacagaga agcctgaaat caaaaaacca ctggctttgt
    46321 tacgcagatg ttttggccag ctttcaatga cataggtggc gctggaaccg tattcacccg
    46381 ccatccccat gccgatgacc agacgagcga taaacatggt gatgtagcct ggcgcaaagc
    46441 cgcaggccag cgtcccggcc gagaagagaa cgatgctggt gaccattgcc agacgacgcc
    46501 cgtagcggtc acccatagcg ccgagcatca ggccgccgaa ccagcgagag ataaaggctg
    46561 cggagatcag acttgccgcc tgcaccgtcg tcagcccgaa ttcaccctgt acttcggtga
    46621 gtaccagggc gattaaaacg aaatcaaaac cgtcaagcag atatcccaac caggcagcgg
    46681 aaaatgcgcg ccattgtgca cggttgagat ggcgatacca cgggatattc tgggttgtag
    46741 tactcattgt gagtctcccg cggtgggcga tgcccacacg ctttggtatg aaaattgtag
    46801 ggtacagatg cgtttatttc ccctcacccg gtaggggcga gcgaggggaa aacaactcac
    46861 ccgcgctctt gcatcaactg ctgggccagc gccttcagtt ctggcagata tttttcatct
    46921 accggtccaa acggtttgcg gcacagcggc acagaaacga catccatata atgcaggaca
    46981 gttttcaggc cgcggaatac gccagttttg atcagtaaat caatgacttt gttgcactca
    47041 gtttgcagtt tctgcgcggt ctggatatcg ccttctttca gcgccttcac gatcccctga
    47101 tatcgccagc ccatgatgtt gtaagtactg ccgataccac catcagcgcc tgccagcaag
    47161 ccggaagcga agatttcgtc gtaaccgtta tagagcacca gatcaggatg ttcacgacgg
    47221 atctgttcca tctgatagag atcgccagaa gtctgtttca gcgcaccgac gccaggcaac
    47281 gtaaccaggg tgttgatctg atccagggtc agttttaccc cactcagggc tggaatgttg
    47341 tacacaacca tcggcaaacc atccgctgaa tcaataattg cccgatagtg atcgcagtgt
    47401 tcttcaaagc tgaaaggata gtagaacggc gtgacggcgg agacggcatc gaagccataa
    47461 cgtttagccg atgccgcaag ttgttggctt tcggcggtgc tgacgcaacc gacgtgggcg
    47521 atgagtttaa tcttaccttt cgcctcttcg gcgacgattt ccagtacctg ttcacgctcg
    47581 gaaaggcttt gtacaaaggc ctcgccggtc gaaccaccca cgtataaacc gtcgatgccc
    47641 tgctgaatat tgaactgaac caggcgacgc agactcgctt tatccagtgc ttgttgttgg
    47701 tcaaaaggag tcaggagtgc agccattacg ccacgtaaat tcgttgccat aaatacctct
    47761 gaagtgatac ttgtctgata aacgatatac ctttatacct gttataccag atcaattaag
    47821 caacacccca tacagaaagc ttataatgcg atctgcttca ctaaagtggc attatttctt
    47881 tttgttggtg gtctgaccga aagcgtgcca ggtagcagag acgctgttga gatgcgattg
    47941 caacgcacga tcggcttcgt caggatcatg acggcggatc gcatcaacga tcgcaatatg
    48001 ctgttgatag ctaacgttgt tatgctcgtg cagagcctga tcagccacgg ttggacgtgc
    48061 ggcgatcagc cagtcgagca gggcaacgtg gatcgccatg aagattgggt taccagggat
    48121 ctccgccagc acgcggtgga aatcaacgtc tgaacgaatg aatgccgcgt tgttatccag
    48181 cgactgactg ttgatttcca gtgcttttgc cagcaaatcg atttgctcat cggtggcatg
    48241 ttcagccgca tagcgcacca gactggattc aaagaacaga cgtaattgtt cgaaatgggc
    48301 aatcccaccg ggatgagaaa ggaaatcttt cgccatgccg gaaaactcac cgatgatagt
    48361 gtccgcagaa ggacgcgaga cgcgagcgcg ttcgccgttg tttatttgca ccagaccttt
    48421 gcgttttaac gctgccagcg cttcgcgcac agaaggacgc ccgacgttaa agaacgccat
    48481 cagttcgcgt tcagacggta attgttcacc ttcgccaaat tcacgacggc ggatcatctg
    48541 ttccagctct tcttccacca tttcggagag ttttttacgc gccagcgggc ggctacgcaa
    48601 gttgcgacca attgcaggtg aagaatcttc ggtttgcgaa tcaaatgcgt tcataaggcc
    48661 cattctgtaa ggtcagtgtg attaacatca tcagtgacat cctatcacag gattgaaagt
    48721 aggggaaaat ggcaggtttt tctctttgtg cctcatcatt accataatta acggaataat
    48781 taactattgc gaaaaattaa tgtaacgcag ataaacacat cccgattgaa ttattcataa
    48841 gactattcac aagcattatg aatattatga atgtgttctt acaaaataat cataagcgca
    48901 tattttttaa tgaaaaatca catcacctac aattaaaaac acgacatccg caccataaat
    48961 agacttgcaa aaaatataac atcattgttt tcaatctgcc gtttatggga ttgaccgttt
    49021 tcttttgaca cggagttcaa caatgttcgg cataattata tctgtcatcg tattaattac
    49081 gatgggctat ttgatcctga aaaactacaa acctcaggtg gtgctggctg ccgcaggtgt
    49141 cttcctgatg atgtgcggag tctggttagg gttcggtggt gtactcgatc ccgccaaaag
    49201 cagcggctac ttgatcgtcg acatttataa tgaaatcctg cgcatgctgt ccaaccgcat
    49261 tgccggattg gggctgtcga ttatggcggt gggcggttat gcccgctaca tggagcgcat
    49321 aggagccagt cgcgcgatgg tgagcttgct aagccgcccg ttaaaactca ttcgctcgcc
    49381 gtatattatt ctgtcggcaa cttacgtcat cggccaaatc atggcgcagt ttattaccag
    49441 cgcctccggt ctgggtatgt tgctgatggt caccttattt ccgacgctgg tgagtctggg
    49501 agtaagtcgc ctctctgcgg tggcggttat cgcaaccacg atgtccattg agtgggggat
    49561 tctggaaacg aactccattt ttgcagccca ggtcgcggga atgaaaattg ccacatactt
    49621 cttccactac cagcttccgg tcgcctcttg cgtcattatc tcggtggcga tctcccactt
    49681 tttcgtgcaa cgcgcttttg acaaaaaaga taaaaatatc aatcacgaac aggcagagca
    49741 aaaaactctc gataatgtcc cgccgctcta ttacgccatt ttacctgtga tgccgttaat
    49801 cctgatgctc ggctcgctgt tcctcgccca cgtcgggctg atgaagtcag aactgcatct
    49861 ggtggtggtg atgttactaa gtttgacggt gacgatgttt gttgagttct tccgcaagca
    49921 taacttgcgc gaaacaatgg acgatgtgca ggcgtttttt gacggcatgg gtacgcagtt
    49981 tgccaacgtg gtaacgctgg tggtcgcggg tgaaatattt gcgaaaggct taacgacgat
    50041 tggcactgtt gatgcggtta tcaggggtgc ggagcattct ggtctgggcg gtattggcgt
    50101 gatgattatt atggcgctag tcattgccat ttgtgccatt gtgatgggct ctggcaatgc
    50161 gccgtttatg tcatttgcca gtcttattcc gaatatcgca gccggactac atgtaccagc
    50221 ggttgtaatg attatgccga tgcattttgc cacgacgcta gcgcgcgcgg tttcgccgat
    50281 tactgcggtg gtgaagcgga cagcgatccc catggcagtc ggtttcgtgg tgaatatgat
    50341 tgccacaatc acgctatttt attaagtcat taaaaagaca aaacaggccg cctgggcctg
    50401 ttttgtatta cttcacaaca cgtaatgccg gtcgaccacc gcgtggtggc tgcggaggtt
    50461 catcgtcagg atgagtgtca tcatcgtgat ctggcttgtc gccatcaata accgacataa
    50521 cggtttcgtt gtctgccgat gcctcttcat cattcatgat gctggtatct tcatcgtagg
    50581 cagcttcagg ctcaaacatc gtgcctgcgc cattttcacg ggcgtagata gccagcacgg
    50641 cagccagcgg cacagaaacc tgacgcggaa tgccaccaaa gcgcgcgtta aagcgcacct
    50701 catcattcgc cagttccaga ttgccgacag cacgcggcgc aatgttgagt acgatttgcc
    50761 cgtcacgcgc atattccata ggaacctgca cgccagggag cgtcacatcc accaccaggt
    50821 gcggcgtgag ctggttatcc agcaaccact catagaatgc acgcagcaga tagggacgac
    50881 gtggtgttag ctgtgacaaa tccatacaga ttaactccgg cccagacgca tttcacgttc
    50941 tgcttcagtt aaagaagcaa ggaaagagtc acgctcaaag acgcgggtca tatagccttt
    51001 cagctctttc gcacccgggc cgctgagctc gatacccagt tgcggcagac gccacagcag
    51061 cggagcaaga tagcaatcga ccaggctgaa ctcatcgctc aggaagtacg gcttctgacc
    51121 gaagaccggc gcaatcgcca gcagttcttc gcgcagttgc ttacgtgcgg catctgcttc
    51181 agaagctgaa ccgttgatga tggtgttcat cagcgtgtac cagtcttttt cgatgcgatg
    51241 catgtacaga cggctttcac cgcgagctac cgggtaaaca ggcatcagtg gcggatgcgg
    51301 gaaacgctca tccagatatt ccataatgat gcgagattcc cacagggtca gctcacgatc
    51361 caccagggtc ggaacgctct gattcgggtt gaggtcaatc agatcctgag gcggattgtc
    51421 cttttccacg tgttcgatct cgaaacttac acctttctca gccagcacaa tgcggacctg
    51481 atggctatag atgtcagtag gaccggaaaa cagcgtcatt accgaacgtt tgttggcagc
    51541 gacagccatg aaaacctcca ggtatagtca gaatttttac tgctaccagc caccaggtgg
    51601 ccagtcagaa gttgtgttac ccaataagga acgactctct ttgttcgaaa atcaaacaaa
    51661 aaatgagcaa tacccgacat ttgggcagaa aattggatga tagtttacca gattttgcga
    51721 ccattgtggt gagtggatgc cggaaatggg gaaaaagaga tgcgctttag tctgaaatag
    51781 ttgacttaga cccttattgg cgatgtggtt tttgttttgc ctgtctttca gacggcagca
    51841 aaaagcaact tcccattttt tacgctgatt cagaatttag ccataaaaaa acccgccgaa
    51901 gcgggttttt tcgtaaattg ttttctgccg gagcagaagc caattaacgt ttggagaact
    51961 gcggacgacg acgtgcttta cgcagaccga ctttcttacg ttcaacctga cgagcgtcac
    52021 gagtaacgaa gccagcttta cgcagttcag aacgcaggga ctcgtcgtat tccatcagag
    52081 cgcgggtgat accgtgacgg atcgcaccag cctgaccaga gataccacca cctttaacgg
    52141 tgatgtacag gtccagtttc tcaaccatgt cgaccagttc cagcggctga cgaactacca
    52201 tgcgggcagt ttcacgaccg aagtactgtt ccagagaacg ttggttgatt acgattttac
    52261 cgttgcccgg tttgatgaaa acgcgagctg cggaattttt gcggcgacca gtgccgtagt
    52321 attgattttc agccattgcc tataatcccg attagatgtc aagaacttgc ggttgctgtg
    52381 ccgcgtggtt gtgctcgtta cccgcgtaaa ctttcagttt acggaacata gcacgaccca
    52441 gcgggccttt tggcaacatg cctttaaccg cgatttcaat cacacgctca ggacggcgag
    52501 caatcatctc ttcaaagctc acttgtttga taccaccgat gtggccggtg tggtgatagt
    52561 acactttgtc agtacgcttg ttgccggtta cagcaacttt gtcagcgttc agaacgatga
    52621 tgtaatcacc ggtatctacg tgcggagtgt attccgcttt gtgcttaccg cgtaggcgac
    52681 gagccagttc agtagccaga cggcccagag ttttaccggt cgcgtcaaca acataccagt
    52741 cgcgttttac ggtttctggt ttagctgtaa aagttttcat taaaagctta cccaataaat
    52801 agttacacgt tggtgaacac ccaaacgtct tcaattgttg aggttcacac gacaaagtcc
    52861 ggcaaaccta ccccttcgaa tagcctatgc cagcacacaa aaagttttgg gaaaaaaact
    52921 ttcttgtaac gtggggtcgc aggattatag agaagtcggg gtcaaagatc gacccctttt
    52981 tgtgatttgt gacaggtttt aacccgccaa atgctcgcgc ttcagatact cttcgctttg
    53041 catctcttgc agacgtgaca ggcaacgctg gaactcaaac ttcagccgat cgccctgata
    53101 aatttcatac agcggcactt ctgcactcac cactaatttg acatggcgct cgtaaaactc
    53161 atccaccagc gcaataaagc gccgcgcttc gctctccatc aaccgcgtca taactggtac
    53221 atcaaacaac atgaccgtat gaaagagacg tgagagcgca atatagtcat gctgactgcg
    53281 ggcgtcgacg cacagcgtag taaaagagac cgccagcgtc tggttctcga cgcccattgt
    53341 cgctaatggc cgatggttga tttctaacgt cggtgaattt tctcgtttcc cccccgccag
    53401 cgccaaccat agtttatcca tttgcgcccg ggtttcatcg tggagtggcg aaagccacag
    53461 atgcgcctga gtgagtgtac gcagtcgata atcaacacca gcgtccacgt tcattacatc
    53521 acaatgctgt ttaatggcat cgattgcagg cagaaaacgc gcacgttgca agccatttcg
    53581 ataaagttcg tccggcggaa tatttgacgt cgctaccagg gtaataccgc gggcgaacaa
    53641 ggctttcatc agaccgccaa gtagcatggc atcggtaata tcagaaacaa aaaattcgtc
    53701 aaaacagagc acgtcagttt cggctttaaa gcgatcggca ataatttcca gcggatcggt
    53761 ctgcccctgt aaggcagtta gctcttcatg cacccgcagc ataaaacggt gaaagtgcag
    53821 gcgctgtttc cgctctcccg gcaggctttg atagaaaagg tccatcagcc aggttttccc
    53881 gcgtcctaca ccgccccaca tatataagcc acgcactggc gtatgctttg tgtcttcgcg
    53941 tttaccccac agcttaccga cccgcgccat tagcccactc gtcctggggg ctggtggcgt
    54001 gctattgatg agttcctgat aaataatttc caggcggctg acggcctctt tttgaacgtc
    54061 gtcgggttga tggctgcctt cattgagcgc cttcaggtat tgcgatgttg gggtaacgct
    54121 ttgcatgatc ttattgttat tccttgaata atcggtgcgc cgttgttcac ggttgacgaa
    54181 aaaaaggccg ttctacacta cgcgatatgc agtcgggatt ccacttctgt ggaattaacg
    54241 gttatagtgg cataatcagc cgcaggcatg gagcctgaag ccaacaccct acggaaacaa
    54301 aagacaacgg gagatgttca tgacctggga atatgcgcta attgggttag tcgtcggcat
    54361 cattattggt gctgtggcca tgcgttttgg taatcgtaaa ctacgccagc aacaggcgtt
    54421 gcagtacgaa ctggaaaaga ataaagctga actggacgag tatcgcgaag agctggttag
    54481 ccactttgcc cgcagcgcgg aattactgga taccatggcg cacgactatc gccagctgta
    54541 tcagcacatg gcgaaaagct ccagcagcct gctgccggaa ctgtctgctg aagcaaaccc
    54601 gttccgtaat cgtctggcag agtctgaagc cagcaacgat caggcaccgg tgcagatgcc
    54661 tcgcgactat tctgaaggcg catccggcct gctgcgtact ggcgcgaagc gcgactaatt
    54721 tatttttcgg gcgcagccat tgcgccctcc tcttctctcc ctccccgact atcatttaat
    54781 ctggtgtctc attgttagcc gtctgaaaat tcaataacat caaactgttt tgaatctctt
    54841 ttcttatcat tcaggtacga gagcaggaat aatgaaaaaa caaccccagc tgttgagtgc
    54901 attagcgtta agtgtcgggt taactctctc ggcgtcattt caggccgtcg cgtcgattcc
    54961 aggccaggtt gccgatcagg cccctctccc cagtctggct ccaatgctgg aaaaagtgct
    55021 tccggcagtg gtgagcgtac gggtggaagg aacggccagt cagggacaga aaatcccgga
    55081 agaattcaaa aagttttttg gtgatgattt accggagcaa cctgcacaac ccttcgaagg
    55141 tttaggctcc ggtgtcatca tcaacgccag taaaggctat gtgctgacca acaaccatgt
    55201 gattaatcag gcacagaaaa tcagtattca gctcaatgat gggcgcgagt ttgatgcaaa
    55261 actgattggt agcgatgacc agagcgatat cgccctgtta caaattcaaa acccgagcaa
    55321 attaacgcaa atcgctattg ccgactccga taaattgcgc gtcggtgatt ttgccgtagc
    55381 ggtcggtaac ccatttggcc ttgggcaaac cgccacctct ggcattgttt ccgcattagg
    55441 acgcagcggg ttgaatcttg aaggtctgga aaactttatc cagacagatg cttccattaa
    55501 ccgcggtaac tccggcggtg cactgttaaa ccttaacggt gagttaattg gcatcaacac
    55561 tgcaatcctt gcgcctggcg gcgggagcgt cgggattgga tttgccatcc ccagtaatat
    55621 ggcgcgaaca ctggcgcagc agcttatcga ctttggtgaa atcaaacgcg gtttgttagg
    55681 catcaaaggc accgagatga gtgccgatat cgccaaagcc ttcaaccttg acgtgcagcg
    55741 tggcgcgttt gtcagcgaag tgttgccagg ttctggctcg gcgaaagcgg gcgtcaaagc
    55801 gggcgatatt attaccagcc tcaacggcaa accgctgaat agctttgctg agttgcgctc
    55861 tcgtatcgcg accaccgagc cgggcacaaa agtgaaactt ggcctgctgc gtaacggcaa
    55921 accactggaa gtagaagtga cgctcgatac cagcacctct tcgtcggcca gcgctgaaat
    55981 gatcgcgcca gcgctggaag gtgcaacgtt gagcgatggt cagctaaaag atggcggcaa
    56041 aggtattaag atcgatgaag ttgtcaaagg aagcccagct gctcaggctg gcttgcaaaa
    56101 agacgatgtg atcattggcg tcaaccgcga tcgggtgaac tcgattgctg aaatgcgtaa
    56161 agtgctggcg gcaaaatcgg ccatcatcgc cctgcaaatt gtacgcggca atgaaagcat
    56221 ctatctgctg atgcgttaat gtcgtaaacc gggcatcagg cttacgtgtg atgtccggtt
    56281 aactcgtggt atgctgctgc cgttcccttt tttaatgacg cctccatcat gtttgtgaag
    56341 ctcttacgtt ccgttgcgat tggattaatt gtcggcgcta ttctgctggt tgccatgcct
    56401 tcgctgcgca gccttaaccc gctttccact ccgcaatttg acagtaccga tgagacgcct
    56461 gccagctata atctggcggt tcgccgcgcc gcgccagcgg tggttaacgt ttacaaccgt
    56521 ggtttgaaca ccaactctca caaccagctt gagatccgca ccctgggatc cggtgtaatc
    56581 atggatcaac gcggttatat catcaccaat aaacacgtca tcaacgacgc cgatcagatc
    56641 atcgtcgcct tacaggatgg tcgtgtattt gaagcattgc tggtgggatc tgactctcta
    56701 accgatctgg cggtacttaa aattaatgcc actggcggtt tacctaccat tccaatcaat
    56761 gcacgtcgcg taccgcatat tggcgacgtg gtactggcga tcggtaaccc gtacaacctc
    56821 gggcagacca ttacccaggg gattattagt gccacgggtc gaattggtct gaacccgacc
    56881 gggcggcaaa acttcctgca aaccgatgct tccattaacc acggtaactc tggcggcgcg
    56941 ctggtgaact cgctgggcga actgatgggc attaacacgc tgtcgtttga taagagtaac
    57001 gatggcgaaa cgccggaagg tatcggcttt gcgattcctt tccagttagc aaccaaaatt
    57061 atggataagc tgatccgcga tggtcgcgtg atccgcggct acattggcat tggcggacgt
    57121 gagatcgcac cgctgcacgc acagggcggt ggtatagatc aactgcaagg gatcgtggtt
    57181 aatgaagtgt cacctgacgg cccggcggcg aatgcgggta ttcaggtcaa cgatctgatt
    57241 atttcggtgg ataacaaacc ggccatctct gctctggaga cgatggatca ggtggcggaa
    57301 attcgccctg gttcggtgat cccggttgta gtgatgcgtg atgataagca gttaacgctg
    57361 caggtcacca ttcaggaata tccggcaacc aattaagtcg agtgctcaaa acaaaaaacc
    57421 ggagtctgtg
//
LOCUS       DAZPEP010000010        55730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZPEP010000010 DAZPEP010000000
VERSION     DAZPEP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.010"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="ACHUT9_000614"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="ACHUT9_000614"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1347176.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUT9_000615"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="ACHUT9_000615"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFR1347177.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUT9_000616"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="ACHUT9_000616"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFR1347178.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUT9_000617"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="ACHUT9_000617"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFR1347179.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUT9_000618"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="ACHUT9_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFR1347180.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUT9_000619"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="ACHUT9_000619"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFR1347181.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUT9_000620"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="ACHUT9_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFR1347182.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUT9_000621"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="ACHUT9_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFR1347183.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="ACHUT9_000622"
     CDS             6537..6773
                     /locus_tag="ACHUT9_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFR1347184.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="ACHUT9_000623"
     CDS             6776..7633
                     /locus_tag="ACHUT9_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFR1347185.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="ACHUT9_000624"
     CDS             7654..9645
                     /locus_tag="ACHUT9_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFR1347186.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUT9_000625"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="ACHUT9_000625"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFR1347187.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="ACHUT9_000626"
     CDS             complement(10248..11144)
                     /locus_tag="ACHUT9_000626"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFR1347188.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="ACHUT9_000627"
     CDS             complement(11193..11432)
                     /locus_tag="ACHUT9_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFR1347189.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUT9_000628"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="ACHUT9_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFR1347190.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUT9_000629"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="ACHUT9_000629"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFR1347191.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMEE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUT9_000630"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="ACHUT9_000630"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFR1347192.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUT9_000631"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="ACHUT9_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFR1347193.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="ACHUT9_000632"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="ACHUT9_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUT9_000633"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="ACHUT9_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFR1347194.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUT9_000634"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="ACHUT9_000634"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFR1347195.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUT9_000635"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="ACHUT9_000635"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFR1347196.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="ACHUT9_000636"
     CDS             complement(21570..22739)
                     /locus_tag="ACHUT9_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1347197.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUT9_000637"
                     /gene_synonym="ynhF"
     CDS             complement(22905..22994)
                     /gene="cydH"
                     /locus_tag="ACHUT9_000637"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFR1347198.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="ACHUT9_000638"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="ACHUT9_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFR1347199.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUT9_000639"
     CDS             complement(24315..25247)
                     /gene="punR"
                     /locus_tag="ACHUT9_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFR1347200.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /gene="punC"
                     /locus_tag="ACHUT9_000640"
     CDS             25360..26571
                     /gene="punC"
                     /locus_tag="ACHUT9_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFR1347201.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUT9_000641"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="ACHUT9_000641"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFR1347202.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUT9_000642"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="ACHUT9_000642"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFR1347203.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUT9_000643"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="ACHUT9_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFR1347204.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNKKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="ACHUT9_000644"
     CDS             complement(30320..31576)
                     /locus_tag="ACHUT9_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1347205.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="ACHUT9_000645"
     tRNA            31884..31960
                     /locus_tag="ACHUT9_000645"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="ACHUT9_000646"
     tRNA            31965..32041
                     /locus_tag="ACHUT9_000646"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="ACHUT9_000647"
     CDS             32149..32454
                     /locus_tag="ACHUT9_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFR1347206.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="ACHUT9_000648"
     CDS             32580..34184
                     /locus_tag="ACHUT9_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFR1347207.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUT9_000649"
     CDS             complement(34196..35008)
                     /gene="ydhT"
                     /locus_tag="ACHUT9_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFR1347208.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUT9_000650"
     CDS             complement(35012..35797)
                     /gene="phsC"
                     /locus_tag="ACHUT9_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFR1347209.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="ACHUT9_000651"
     CDS             complement(35794..36462)
                     /locus_tag="ACHUT9_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1347210.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="ACHUT9_000652"
     CDS             complement(36526..37164)
                     /locus_tag="ACHUT9_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFR1347211.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39279)
                     /locus_tag="ACHUT9_000653"
     CDS             complement(37177..39279)
                     /locus_tag="ACHUT9_000653"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFR1347212.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(39300..39926)
                     /locus_tag="ACHUT9_000654"
     CDS             complement(39300..39926)
                     /locus_tag="ACHUT9_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFR1347213.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(40382..40591)
                     /gene="fumD"
                     /locus_tag="ACHUT9_000655"
     CDS             complement(40382..40591)
                     /gene="fumD"
                     /locus_tag="ACHUT9_000655"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFR1347214.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            40946..41140
                     /locus_tag="ACHUT9_000656"
     CDS             40946..41140
                     /locus_tag="ACHUT9_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347215.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            41148..42560
                     /gene="pykF"
                     /locus_tag="ACHUT9_000657"
     CDS             41148..42560
                     /gene="pykF"
                     /locus_tag="ACHUT9_000657"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFR1347216.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            42870..43106
                     /gene="lpp"
                     /locus_tag="ACHUT9_000658"
     CDS             42870..43106
                     /gene="lpp"
                     /locus_tag="ACHUT9_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFR1347217.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(43169..44173)
                     /gene="ldtE"
                     /locus_tag="ACHUT9_000659"
     CDS             complement(43169..44173)
                     /gene="ldtE"
                     /locus_tag="ACHUT9_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFR1347218.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(44322..44738)
                     /gene="sufE"
                     /locus_tag="ACHUT9_000660"
     CDS             complement(44322..44738)
                     /gene="sufE"
                     /locus_tag="ACHUT9_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFR1347219.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(44751..45971)
                     /gene="sufS"
                     /locus_tag="ACHUT9_000661"
     CDS             complement(44751..45971)
                     /gene="sufS"
                     /locus_tag="ACHUT9_000661"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFR1347220.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(45968..47239)
                     /gene="sufD"
                     /locus_tag="ACHUT9_000662"
     CDS             complement(45968..47239)
                     /gene="sufD"
                     /locus_tag="ACHUT9_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFR1347221.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(47214..47960)
                     /gene="sufC"
                     /locus_tag="ACHUT9_000663"
     CDS             complement(47214..47960)
                     /gene="sufC"
                     /locus_tag="ACHUT9_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFR1347222.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(47970..49457)
                     /gene="sufB"
                     /locus_tag="ACHUT9_000664"
     CDS             complement(47970..49457)
                     /gene="sufB"
                     /locus_tag="ACHUT9_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFR1347223.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(49466..49834)
                     /gene="sufA"
                     /locus_tag="ACHUT9_000665"
     CDS             complement(49466..49834)
                     /gene="sufA"
                     /locus_tag="ACHUT9_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFR1347224.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(50382..50570)
                     /locus_tag="ACHUT9_000666"
     CDS             complement(50382..50570)
                     /locus_tag="ACHUT9_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFR1347225.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            complement(50670..51080)
                     /gene="menI"
                     /locus_tag="ACHUT9_000667"
     CDS             complement(50670..51080)
                     /gene="menI"
                     /locus_tag="ACHUT9_000667"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFR1347226.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(51077..54133)
                     /locus_tag="ACHUT9_000668"
     CDS             complement(51077..54133)
                     /locus_tag="ACHUT9_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFR1347227.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            54522..55634
                     /gene="ydiK"
                     /locus_tag="ACHUT9_000669"
     CDS             54522..55634
                     /gene="ydiK"
                     /locus_tag="ACHUT9_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFR1347228.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
BASE COUNT        13415 a        14653 c        13644 g        14018 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcga
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgtg caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggagg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc catttttagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaggg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtggca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gacgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcgaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaagcac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccaa
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacaaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc
    38041 cagttgatcc cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacacc
    38101 tttgctgtaa caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta
    38161 gtcatcgaac agattcagac ccaccaggtt accaatcaca cgaccatcgt ctttatcttc
    38221 aaaatctttc ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt
    38281 accacctgtg ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca
    38341 acggatcggg caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc
    38401 taagtcaaaa acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat
    38461 ttcccccgtt tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt
    38521 ccaacgtgac ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg
    38581 gttgttattc gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg
    38641 acgatcggca atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga
    38701 acccattatt gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga
    38761 gagaggaaca aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt
    38821 caatcgacaa atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt
    38881 ttccaggcta actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc
    38941 gataataatg acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg
    39001 ggcatcgacg actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga
    39061 actacagggg gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc
    39121 atcgaaaggt ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat
    39181 gccaccgaca aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag
    39241 attgactctt aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt
    39301 cacacagtaa tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg
    39361 cattcaccgc ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc
    39421 atccacgggc atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta
    39481 atacagcctt ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc
    39541 ggctctttgc attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg
    39601 ccagagccaa ccccgttgtc gccaaagaaa taattgcgat ggattttgat acgggagaag
    39661 aatgtcccta ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc
    39721 gtgcatcgcg ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg
    39781 ccgctatcaa tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt
    39841 agaccagcaa ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct
    39901 aatagtggac gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga
    39961 caattcagca gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac
    40021 cagatatttt ccctaacccg atagtgtatg agaaaagatt taacgccgat aaaccaatcc
    40081 tttatggtta ggagaaaaaa taatgtgatt attctgagcc cttaacattg atcgttatca
    40141 attaaaatta caaagcaaga aattataagt gaactgatat ttattatcaa ttgaaataaa
    40201 tttaacttaa gcgacaatta tttgtgacgt tcattgcaga aaataaaaat aatattatcg
    40261 ttatagatta aaatacatac cgcaattcgt ataatttcag cagttgttat ttagaatgat
    40321 taactggacc gcaactgaag caagaatatc aggatgaagc ttaccgcctc atcctgaatg
    40381 gtcattttac cagtgcgtta atgacggttt ccatcgcctg cttttccagc tcactgcgct
    40441 gaatgcgttt attggctaat gcagtacgta atacgcctgc gataacaata tgctttggtt
    40501 cctgccccag atcgcgcatt tccagcacaa ctttaccgac aactctgcac atttctcggt
    40561 acagttcatc ctcttttgtc cgatttccca ttctttatac ctatttatca tgccaactat
    40621 cagcatatat caatctaacc aattaaacaa aaatcaaaca aaatcagaca aataacgcga
    40681 taaattattt taactgtagc aattgagcga tgatatattt atacaccgga tgaactttca
    40741 cttatcctca cactgacaac ttcggcacca gacgttgcgc aaacagcgaa gtttttgcgt
    40801 aaccttttcc ctggaacgtt aaatctttga taacaattta ttgtctaaca agttgtatat
    40861 tttttgaaac gctgtttttg ttttcctttt ggattaattt cagcgtataa tgcgcgccaa
    40921 ttgactcttg aatggtttca gcactttgga ctgtagaact caacgactca aaaacaggca
    40981 ctcacgttgg gctgagacac aagcacacat tcctctgcac gctttttcga tgtcacctat
    41041 ccttagagca aggcaccacc actttcgtaa taccggattc gctttccggc agtgcgccca
    41101 gaaagcaagt ttctcccatc cttctcaact taaagactaa gactgtcatg aaaaagacca
    41161 aaattgtttg caccatcgga ccgaaaaccg aatctgaaga gatgttagct aaaatgctgg
    41221 acgctggcat gaacgttatg cgtctgaact tctctcatgg tgactatgca gaacacggtc
    41281 agcgcattca gaatctgcgc aacgtgatga gcaaaactgg taaaaccgcc gctatcctgc
    41341 ttgataccaa aggtccggaa atccgcacca tgaaactgga aggcggtaac gacgtttctc
    41401 tgaaagcggg tcagaccttt actttcacca ctgataaatc tgttatcggc aacagcgaaa
    41461 tggttgcggt aacgtatgaa ggtttcacta ctgacctgtc tgttggcaac accgtactgg
    41521 ttgacgatgg tctgatcggt atggaagtta ctgccattga aggtaacaaa gttatctgta
    41581 aagtgctgaa caacggcgac ctgggcgaaa acaaaggtgt gaacctgcct ggcgtttcca
    41641 ttgccctgcc agcactggct gaaaaagaca aacaggacct gatctttggt tgcgaacaag
    41701 gcgtagactt tgttgctgct tcctttattc gtaagcgttc tgacgttatc gaaatccgtg
    41761 agcacctgaa agcgcacggc ggcgaaaaca tccacatcat ctccaaaatc gaaaaccagg
    41821 aaggcctcaa caacttcgac gaaatcctcg aagcctctga cggcatcatg gttgcgcgtg
    41881 gcgacctggg tgtagaaatt ccggtagaag aagttatctt cgcccagaag atgatgatcg
    41941 aaaaatgtat ccgtgcacgt aaagtcgtta tcactgcgac ccagatgctg gattccatga
    42001 tcaaaaaccc acgcccgact cgcgcagaag ccggtgacgt tgcaaacgcc atcctcgacg
    42061 gtactgacgc agtgatgctg tctggtgaat ccgcaaaagg taaatacccg ctggaagcgg
    42121 tttctatcat ggcgaccatc tgcgaacgta ccgaccgcgt gatgaacagc cgtctcgagt
    42181 tcaacaatga caaccgtaaa ctgcgcatta ccgaagcggt atgccgtggt gccgttgaaa
    42241 ctgctgaaaa actggatgct ccgctgatcg tggttgctac ccagggcggt aaatctgctc
    42301 gcgcagtacg taaatacttc ccggatgcca ccatactggc actgaccacc aacgaaaaaa
    42361 cggctcatca gttggtactg agcaaaggcg ttgtgccgca gctggttaaa gagatcactt
    42421 ctactgatga tttctaccgt ctgggtaaag aactggctct gcagagcggt ctggcacaca
    42481 aaggtgacgt cgtagttatg gtttctggtg cactggtacc gagcggcact actaacaccg
    42541 catctgttca cgtcctgtaa tattgctttt gtgaattaat ttgtatatcg aagcgccctg
    42601 atgggcgctt ttttatttaa tcgataacca gaagcaataa aaaatcaaat cggatttcac
    42661 tatataatct cactttatct aagatgaatc cgatggaagc atcctgtttt ctctcagttt
    42721 ttttatctaa aacccagcgt tcgatgcttc tttgagcgaa cgatcaaaaa taagtgcctt
    42781 cccatcaaaa aaatattctc aacataaaaa actttgtgta atacttgtaa cgctacatgg
    42841 agattaactc aatctagagg gtattaataa tgaaagctac taaactggta ctgggcgcgg
    42901 taatcctggg ttctactctg ctggcaggtt gctccagcaa cgctaaaatc gatcagctgt
    42961 cttctgacgt tcagactctg aacgctaaag ttgaccagct gagcaacgac gtgaacgcaa
    43021 tgcgttccga cgttcaggct gctaaagatg acgcagctcg tgctaaccag cgtctggaca
    43081 acatggctac taaataccgc aagtaatagt acctgtgaag tgaaaaatgg cgcacattgt
    43141 gcgccatttt tttgtctgct atttaccact actgcgtcgc gcgtaacata ttcccttgct
    43201 ctggttcacc attctgcgct gactctactg aaggcgcatt gctggctgcg ggagttgctc
    43261 cactgctcac cgaaaccgga taccctgccc gacgatacaa cgctttatcg actaacttct
    43321 gatctacagc cttattgtct ttaaattgcg taaagcctgc tggcagtgtg tatggcattg
    43381 tctgaacgtt ttgctgttct tctgctgata gtggtcgatg tacttcaaca taacgcatcc
    43441 cgttaggttc cacggaatat ttcaccggtt cgttgatcac tttcaccggc gttcccgtcc
    43501 gcacgctgga gaacaaggct ttaatatccg gtgcattcat gcgaatacac cctgaactga
    43561 cgcgcaaacc aacgctgtcc ggcgcactgg tgccatgaat gaggtattcg ccattaccat
    43621 gcgcgaggcg cagtgcgtaa cgtcctagcg ggttatttgg tccggcagga acgactggcg
    43681 gtaatttaat gccacgctcc agcgaacgct gacgaatgcc tgccgtaggc gtccaggttg
    43741 ggttagggat tttctgccca acacgcgttt ccatcaccgg cgtttccagc ccctgcaatc
    43801 caatacctat tggataaacc tgcacaatat tttctcccgg cggataataa taaaggcgca
    43861 gctctgcaag gttaacgata atcccctgac gcggtgcatc aggtaataac agttgtgaag
    43921 gaatagttat cgtcgtacca ggttttggca ccgggacgat agtgttattg gcttcaagga
    43981 tcaacattgc cgcagtatca aaacgtcggg caatagcctg aaggttttta tccccttctt
    44041 gcaccgtata cgtttgattt tgcccaacca gtcggcttcc ggttggtggt agcggataat
    44101 caaccgccca ggcagcctgg atggcgctaa aagcgccgat aagcgtgagt gtaagcaaag
    44161 acgcgcgttt cattgtaaac ctcctgtatt tgccggagac tcacgctgaa acgtcggatg
    44221 gcgcttatgt tcacctgaaa ccaaaacact cctgtgcagg tcagtgtaaa cattgaccat
    44281 ccggcaatgt gagccaaccg gatgaaagct gtccctttag tttagctaag tgcagcggct
    44341 ttggcgcgaa ttgcgcgaat catcgcttcc agaccttgtg aacgagatgg ggtgagatgt
    44401 tgggtgagtg ccattttttc aaaccacgga cgcacatcga aactgacaat atcctgcggc
    44461 gtcatctgat cgtagagaat aaagactacc gcaataagcc ctttcacaat cgccgcatcg
    44521 ctgtcgccct gcaattcaat aattccgtgc gcattctggc gcatgacaat ccacacctga
    44581 ctctggcagc cctgaatgct attttgtggg cttcggtctt cgtcgcgtaa ttcagtcaga
    44641 cgctggccca gctcaataat gtagagatat ttctcttccc agttggcgca gcgtaaaaaa
    44701 ttacgcagca atttttcttt atccggcaat aaagccatag tgcctccctg ttatcccagc
    44761 aaacggtgaa tacgttgcag gccggtcacc agacgatcca cttcttcatg ggtgttatac
    44821 atggccagcg acgcccgaca catcgcaggg acgttgtaat aggccattaa tggcatagcg
    44881 cagtgatgtc cggtacgcac agcaatgccg taattatcga gaaaactgcc aacatcatag
    44941 gcgtggtgtt taccgagatt aaaagcaata acgccaagcc tgttttgtgg gccatagaga
    45001 gtgagatccg gtacagattc cagctgtgat agcgcataat gcatcagatt ctgttcatac
    45061 tcggctatgt tattaagccc cagcgccgaa acatactcca gcgccgcgcc aagaccaata
    45121 atgcccccgg tattgggtgt accggcttca aaccgccatg gtgctttggt ccaggtagtg
    45181 ccttcactca ggctgacggt ggcgatcata gaaccgcccc cttcccacgg cggcatctcc
    45241 tgcaacaagg cttctttcac ataaagaatg ccaattccgg tggggccata cagtttatgc
    45301 ccggagaaca cgtaaaagtc gcaatccagc gcctgaacat ccaccggatg atgcatcacc
    45361 gcctgagcgc catccaccag cacttttgcg ccatgctggt gcgcaagcgt gattatttcc
    45421 gccagcggat tttctgttcc cagcacgttg gagacatgag taattgccag caggcgagtt
    45481 ttctcatcaa acagcgtagg cagcgtctcc agttgcagcg taccgtcggg gttgagaggg
    45541 atcacacgca gctctgcgcc aacgcgtgcg caaagcatct gccagggaac aatgttagcg
    45601 tggtgctcca tctgactgat gatgatgtta tcgcccgccc gcacgttgct gttgccccaa
    45661 ctattggcga ccagattgat cccttccgtc gtgccgcgga cgaacaccag ctcttccgcc
    45721 gaacgggcat taataaacag cgatgcccgc ttgcgcacgt tctccatttt ctcggtcgcc
    45781 tgggcgctta aggtatgaat accgcgatgc accgccgcgt agccgtgacg ataaaactca
    45841 gcctcggcgt caatcacctg gcttggtttc tgcgcactgg cggcgctgtc gagataagcc
    45901 agcggcaaac cgttcacctc acgagaaagc accggaaagt cggcccgcac tttgtcgacg
    45961 gaaaaagtca tcttgcacct cctggcagcc gttgaccgat tcgggccagc acctgctgtt
    46021 taagcccctc atcacgcagt gcttccgtca gttcggcagc aaaggcgtaa atgatcatct
    46081 gctgggcatc ctgctgattg atcccgcgcg agcgcagata gaacatctgt tcatcatcaa
    46141 tacgccccac cgtcgcgccg tggctgcatt tcacatcatc tgcatagatt tccagctgcg
    46201 gttttgtatc cacttccgcc attttgccca tcagcagatt attgttggtc atctgaccat
    46261 ccgttttgat ggcgtgctgc gcgacgttga tcaaaccgtt aaataccgcg cggcctttgt
    46321 cgctgacgat agttttgtgc aactgtcggc tgttacaaaa acctttattg tgttccagcc
    46381 aggtacgggt atcacacacc tcgtttttca ccggcatcgc caggctattg atccgcagcg
    46441 tgctgttttc gccattgagt tgcgtactgg tgttgtgtcg taacactgcg ccacccagca
    46501 ggaaactatg gctaaatgcg gtggcatcct cagccagcaa caaatcgtta tgagcaaagt
    46561 ggtgactgag cgggttttca aacgccagct tgatatgctg caagtgggcg ttcgctgcga
    46621 cattgatagt gaaccgtgcc ccggtaaaat gacgagcatc attaaggctg acaaaatgtt
    46681 cgatcaccgt tgcttcggca ccttccgcca gatccagatg atgccggtaa tgggcagtgt
    46741 tcacctcttc acctgccacg ccctgggtga tatgcattaa cagcaatggc tttgccggcc
    46801 gttgaccgcg cttcacggcg atatgcgtca cgctttgtgc caggctttcc gtcaaatgca
    46861 gaaacacttc cgcctgaata gcgtcgggta acccctgacg gtcgtcgtta atgctcactt
    46921 catatccgct gccttcaatt gcatcgctca gcgcgggcac gtaacgtcca tcgacaaaca
    46981 ccagccgcac ggcgtctaac gttaacgcta aggcatcacg ctgctgtggg gatatatctc
    47041 ccgcaatgct gacaaactga ctattggtca gcccttccag cggcgtatat ttccagtttt
    47101 catgtttacg tgtcggcagt ccggtacgca gcaattgttg taaatgctgc tgtgcttgcg
    47161 gtgagcgttt tgtcccttca gcttcaaaca agtgatgcca ctgttgcagc gcattactgc
    47221 tgttcggtaa gccagccata accctgctcc tccagttgtt tgaccaacgt gaaatcgccg
    47281 gatttcacaa ttcgtccctg atatagcaca tggacgtaat caggcttgat gtagtcgaga
    47341 atgcgttggt agtgcgtaac aatgatgaat gagcgcttgc cgtcacgcag cgagttcacg
    47401 ccatcggcaa ccacttttaa tgcgtcaata tccagcccgg agtccgattc atcaagaatg
    47461 cataactccg gttccagcac cgccatttgc aaaatatcgt tgcgcttttt ctcgccgccg
    47521 gaaaaaccaa cgtttaccga acgggttaat aaatcttccg gcatcttcag gagagcgatt
    47581 ttctcttcca tcaaatcctg aaaatcaaag cggtcgagcg tttcctggcc gcgatagctg
    47641 cgcaccgcat taagtgccgt ttgcaggaaa aactggttac tgacgccggg aatctccacc
    47701 ggatactgga aggccataaa gatgccttcg cctgcacgat cttccggcga cagcgcaagc
    47761 aaatctttgc ctttgaactc aaccgtgccg cccgtcactt cataatcttc tcgcccggca
    47821 agcgttgccg ataaggtact tttgcccgaa ccgtttggcc ccataatggc gtgaacttcg
    47881 ccgggacgaa cgtcgaggct taatccacgc aggatagctt tatcttccac gctgacgtgt
    47941 aaatctttaa tacttaacat gtttattcct tatccgacgc tgtgttcaag actgatggcg
    48001 aggagttttt gtgcttcaac ggcaaattcc agcggcagtt ctgagaacac gtctttgcag
    48061 aaaccgttaa caatcatcga gatggcgtct tcttcgctaa tcccgcgttg caggcagtaa
    48121 aacagttgat cttcaccaat acgtgatgtc gttgcctcgt gctccagttg tgcgctgtta
    48181 ttgcgacatt cgacatacgg gaaggtatgc gccccacaat tagcgccaat cagcattgag
    48241 tcgcactgag tgaaattgcg cgcattggtt gccgtcggca tgattttcac taagccgcga
    48301 taactgttct gactatgtcc ggcagagatc cctttcgaga taatggtcga tttggtgttt
    48361 ttaccgatgt ggatcatctt ggtgccggta tccgcttgct gatgaccgct ggtcagcgcc
    48421 actgagtaaa actcaccaat ggagttatcg ccgcgcaaaa tgcagctggg atatttccac
    48481 gtaatcgctg acccggtttc tgattgcgtc catgacattt tgctgttttc gccttcgcac
    48541 aaagcacgct tggtgacgaa attgagaata ccgccggtgt tgttatcgcc agggaaccag
    48601 ttttgtaccg tggaatattt cacctcggcg tttttatgga tgatgacttc caccaccgcc
    48661 gcgtgtaact gatagctgtc acgcaccgga gcggaacagc cttcaatgta gctgacgtag
    48721 ctgtcttcgt cggccaccag aatggtgcgc tcaaactgcc cggttttttc cgcgttaatg
    48781 cgaaaatagg tggaaagttc catcgggcag cgcacgcctt taggcacata aataaacgta
    48841 ccgtcagagg ctaccgccgc attaagcgcg gcaaagaagt tgtcatttcc cggcaccaca
    48901 gtgccgagat atttacgcac cagttccgga tgatcgtgga tcgcctcacc aaaggaacag
    48961 aaaataattc cctgctccgc cagtttttcg cgataagtag tggcaaccga aacagagtcg
    49021 aaaatggcat ccaccgccac ctctttgcct tcccgcacgg gaacgcccaa ctgctcaaac
    49081 gccgcctcca cctctttact taaaaaggcg ttcgcgccag tttgctgcac cgcgccaggt
    49141 tcagacgcgc aagtgtcgtc acaattaccg cacgatggtg ctgagtagta actgtaatcc
    49201 tgataattca gcttgtcgta gtgcgctttc aaccagtgcg gttcttccat ctccagccat
    49261 gcgcgatagg cgtttagacg aaactccagc atccactccg gctcattacg cttcgccgaa
    49321 attgcgcgca ccacctcttc gtttatcccc tttgccagct catcggtggc taactgggtg
    49381 aagaatcctt ctttataatt cagcgggccg ccggtccagg ttttgacatc gtcagttgct
    49441 tcagtattac gagacatagt accgcctata ccccaaagct ttcgccacag ccacattcat
    49501 tctgggcttt agggttgtga aatttgaata tctgattaag tccttcacga acgaaatcga
    49561 cttccgtgcc atcaataaac ggcatcgctt gcagcgggac aaacagcttc gcgccgtcgt
    49621 gttcaaacag cagatcgtct ttgtccggct cgctaacact gtcgagcaca tagccaaagc
    49681 ccgcgcagcc cgtttgcttc acgcctaagc gcacgccgac catacccggc tgctttgcca
    49741 ccagctcacg gatgtgtatc gccgctgcgg gtgtcagcgt taagccttgc caggcgaaat
    49801 cttgtgggtt aaaggttcct gaatgcatgt ccatcgattt acctcacttc atcgctttca
    49861 gcgtataaca gcatgctagt gataatgatt atcagttcaa cccagcaaac gcaggggctt
    49921 taccgtaaaa catgcttttt gcctgctttt aataagcata gaccctgatg tgtgggttaa
    49981 caggcacgct aagaattagg tatctcattg ttagataatg gttattatct aaggagcatt
    50041 aaggctgtga tgaaaaagaa aaagttgtat tgaaaatgac tatttaagag ataggtaaaa
    50101 aagtgcagcg ttcagaaata agaaaaccct taagtctgtg cgacacaggc ttaagggttt
    50161 ctaccccatc cggcgcttat ctccggcact ctcagtggct tagctcttga aggggcgata
    50221 agaataatct cataaagcta acccgccgtt ttaacacaaa ctgcgattag tattattttt
    50281 gaacaatatc aggcggtaga taagcagtat taagaaggtc atcgaacctg gacggaggtt
    50341 aatccaggtc gatttggcga acttgcggca ttaagtcagg atcaatgcac gcccagacgc
    50401 caggcaaagt agatttcttc ttttaattca gtagaagaga gagtaagcag gtcagcaaat
    50461 tcaagttcta gttgtttcag acgtttgaga tattgggcag gtgaaagatt gctctggtca
    50521 cggcgtaaaa attcaatggc cagctgggtg ggatcaagtt gagtagacat agcatcctcg
    50581 cttttagaca agacgtgcac agtataccac cgtttactgt gcagataatg accaaaagca
    50641 atatgcgtca cacttttctg gtgacaacgt cacaaaatgg cggtcgtcaa tcgtgacgaa
    50701 cagcacaaac gccctttctc atcgaagatt tcaatctgcc agacctggtg acgcgaaccg
    50761 agatgcaacg gtttacatat gccgcgcacc cgcccttctc gtgccgagcg gacgtgatta
    50821 gcattgattt ccagaccaac cactttttgc tcaccttcgg tacataaata accggcaacg
    50881 gaaccgatac tttcggccag taccacggat gctcctccat gcagcaaccc gaaaggctgc
    50941 tttgtccgcg agtctactgg cattgtcgct tcaagggtgt catcaccaat atgttcaaag
    51001 cgaatatcca ggaaccccac catgtttcct tcacccatag cattcagtgc ttccagggtg
    51061 attttccgtt tccatatcat ttaataatct ccagtaaagc ctgcacagga tggcgtaccc
    51121 ccgtgccttc aacccgtttt acctggctac ggcaggaata tccggtcgcc agacagcggt
    51181 tacgcggcag tcgctgcata gcctgatgcc aggataactc atagatcccg agcgaatttt
    51241 catggttttt cgcttcatgt ccgtaagtcc ctgccatgcc gcagcaaccc acgctgacat
    51301 tttccagttt cgcgccaaaa cgggcaaata tcgcggccca ttgtgctggc gcgcccggca
    51361 aagcggtaac ctcggtacag tgaccaaaga aataccatga ttcaccgctg actgtagcca
    51421 ctggatgtga ctcaagtgcg cttgccagcc attcattcgc aagtaagacg ttaaactcgc
    51481 cacgttcctc gcccagcgcc agtttatatt catcgcgata acaaagtacc agcgccggat
    51541 cgacgcccac cattggcatc ccaagcttag ccatacggtt gaggaaatcc gccgtctttt
    51601 tcgccgtctt cgcaaaacga ttaagaaagc ctttgatatg ctgggctttg ccatttggcg
    51661 aaaatggcag taacacaggc tggaaaccta atttttcaac cagacggaca aaatccgcca
    51721 ccacttgcgc atcgtaatag ctggtaaagg ggtcctgcac caccaacacc gtgcgcgctt
    51781 tctgctctgc attgagagct tcaagctgtt ccagcgtcat gtttgccgag cgatgcccca
    51841 ccatttgttg ttgtagcgag gggaccgaca gcagtggcaa atcaaccatg ccgatatgtt
    51901 tttccgagag tttgcgcacc agcggctggt taatgaagaa gttaaaggtt ttcggcgcgc
    51961 gtgccatcag cggtgcgtag ctctcgaccg tagcgacgag gtggtcgcgc agcgggcgta
    52021 aataacgggt gtgatagagc tgcaggaaac gagagcgaaa ctccggcaca tcaattttga
    52081 tggggcactg ggtcgaacac gctttacagg ccagacagcc cgacatcgcc tctttgactt
    52141 cgtgtgagaa gtcatattcg cctttattcg catgccagct attgcgcgtg cgggcaatta
    52201 acgtccgtaa actgacgccg gattcaggca gttcttgttc cagtttgagt ggatcaacgc
    52261 cgcgatccgc caacaaacgc agccattcac gcaccagcgt tgcgcgccct ttcggtgaat
    52321 gaatccggtt ctgggtgatc ttcatcgacg gacacatcgg actacgggca tcaaagttga
    52381 agcataaacc gttgccgtta cactccatcg caccgcgcca ctgctggcgt accgcaatgg
    52441 ggatctgccg atcgaatgta ccgcgcttca ccgcgtccac tttcatcatc ggcgcatcga
    52501 gaccttctgg cgggcaaatc ttccctgggt tgagtcggtt atgcgggtca aatgccgctt
    52561 tcactttgcg cagttctgca aaaagttcct caccgaaaaa cgccgggctg tattcagcgc
    52621 gaaaaccttt gccgtgctcg ccccacaaca aaccaccgta tttcgcagtc agcgccacca
    52681 cgtcatcaga gatttgcttc atcaaaatct cttgttgcgg atcgcacata tccagtgccg
    52741 gacggacgtg caaaacacct gcgtcgacgt gaccgaacat accgtagctt aagccgtggc
    52801 tgtcgagcag cgcgcgaaat tcagcaatat aatcggccag atgttccggc ggtacgcagg
    52861 tatcctcagc aaacggaatt ggcttagcgg cacctttggc attgccaagc agaccaacgg
    52921 cttttttgcg cattgcatag atacgttcaa cccccgccag ctcgcggcac acctgccagc
    52981 caatcacacc tgcctgttgg ctggcaatca gctcatcaag ccgcacacag agtgcattaa
    53041 ctcgctcgtc aatcagcgcc tcatcatcac cagcaaattc cacaatgttc agcccgagca
    53101 tctctttgtc aggcacatcg gtaatcaact cgctgacgga atgccagaca atatcttccc
    53161 gcgccagatt cagcactttt gagtccaccg tctctaccga aagcgcccgc gcctcaacca
    53221 taaacggcgc gttacgcagc gcggagtcaa aagagtcata tttgacgttc accagacggc
    53281 gcactttagg caagggcgta atatccagcc gcgcttcggt aataaaggcc agcgtccctt
    53341 ctgaacccgt cagaatgcgc gtcaggtcga actcggtcat ctcatcgtta aagacatgac
    53401 gcagatcgta accggtaaga aagcggttaa gtttggggaa gttgtcgatg attaactggc
    53461 gttgcagacg gcaacgttga taaaccgtgt tataaattcg cccgattgtg gtattggttt
    53521 tacccagcgt ttccgccagt tcgacgggta aaggttgcgt atcgagaata tcgcccccca
    53581 acaacaccgc gcgtacgcca agtacgtgat ctgacgtttt gccatagacc agcgatccct
    53641 gaccggatgc atcggtattg atcatcccgc cgagcgttgc ccggttgctg gtcgaaagtt
    53701 ctggtgcaaa aaagtagccg aacggtttca ggtactgatt gagttgatct tttatcaccc
    53761 cggcctcaac gcgcacccag ccctcttcag ggttaatttc gatgatgcgg ttcatatggc
    53821 gggacatatc aacaataatc ccctggttga gcgcctgacc gttagtgccg gtgccgccgc
    53881 cgcggggggt aaagatcagc gatgaatagc gttcctgcgc ggcaagacgg gcgatcagcg
    53941 ccacatctgc ggttgaacgc ggaaatacca ccgcatcggg gagaagttgg taaatactgt
    54001 tgtcggtcga cattgtcaga cgatcggcat aacttgtcgc cgtatcgccg gtaaaacctt
    54061 gttgctccag ctcttgcaaa aaattaagca ccagttgaac gacgccgggt gcctgggaaa
    54121 tctgtggaat cattatattg accctttcct gcggtctgtg atgtaggtcg atacactatt
    54181 ctttcaggcg gctgcaatag cgcactgaaa ggtgatgttt atttactcta tggatttcga
    54241 gttgcaggaa ggcggcaagc gagtgaactc caggagctta caatagtaag tgactggggt
    54301 gaacgaacgt agccgcagcg catgcaactt gaaatacgac gagtaaatcg tttgcgtgtt
    54361 gcctgagttg ttgtaccaca tttttttcta acacgcccat cagaattaag ggcagaatcg
    54421 gcctgttaaa aaccgctgaa attgctcatc attatgcagg tgagtttcgc gtgttcacgt
    54481 cgcgtcgacg atttgacgca caaaaaaggt gaaaagtagt tatggtaaat gttcgtcagc
    54541 ccagggatgt cgcacaaatt ctgctttcgg tgctgttttt agccatcatg attgtggcat
    54601 gtctgtggat tgttcaaccc tttattctcg gctttgcatg ggccggtacg gtggttatcg
    54661 ccacctggcc ggtattgtta cgtttgcaaa agatcatgtt tggccgccgc tctctcgccg
    54721 ttctggtgat gacgctgtta ttagtgatgg tgtttatcat ccctattgct ttgctggtta
    54781 acagtatcgt cgacggcagc ggcccgctaa ttaaagccat ttccagcggc gacatgacgt
    54841 tacccgatct ggcgtggctt aataccattc cggtgattgg cgcgaagctg tatgcaggct
    54901 ggcacaactt gctggatatg gggggtacgg cgatcatggc gaaagtccgc ccttatattg
    54961 gcaccaccac cacctggttc gttgggcagg cggcgcatat cgggcgcttt atggtgcatt
    55021 gtgcgctgat gcttctcttc agtgccctgc tgtactggcg cggtgaacag gtggcacaag
    55081 gcattcgcca ttttgcaacc cgtctggcag gcgttcgcgg agatgccgcc gtgctgctgg
    55141 cggcacaggc tatccgcgcg gtggcgctgg gtgtggtggt gacggcgtta gtacaggcag
    55201 tgcttggcag tatcggcctc gccgtatccg gcgtacctta tgcaacgttg ctaacggtgt
    55261 taatgatcct ctcctgcctt gtccagcttg gcccgttgcc ggtactgatt ccggcgatta
    55321 tctggctcta ctggaccggc gataccacct ggggaacggt attgttagtg tggagcggtg
    55381 tggttggcac gctggataac gtcatccgcc caatgttaat tcgcatgggt gccgatttac
    55441 cgctgatcct gatcctctct ggcgttattg gtggtttgat tgctttcggg atgatcggtc
    55501 tgtttattgg tccggttctg ttagccgttt cctggcgtct gtttgccgcg tgggtggaag
    55561 aagtcccgcc gccgacggac caaccggaag aaattctcga agaacttggc gaaatcgaga
    55621 aaccgaataa gtaatttctc atcaggcggc tctgccgcct gattgttaat caccgctaat
    55681 tgatatttgc ttgctcttcc ccatattttc ctgcttaccg ccattcagct
//
LOCUS       DAZPEP010000011        49281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZPEP010000011 DAZPEP010000000
VERSION     DAZPEP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.011"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            252..1346
                     /gene="gcvT"
                     /locus_tag="ACHUT9_000670"
     CDS             252..1346
                     /gene="gcvT"
                     /locus_tag="ACHUT9_000670"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFR1347229.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            1370..1759
                     /gene="gcvH"
                     /locus_tag="ACHUT9_000671"
     CDS             1370..1759
                     /gene="gcvH"
                     /locus_tag="ACHUT9_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFR1347230.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            1878..4751
                     /gene="gcvP"
                     /locus_tag="ACHUT9_000672"
     CDS             1878..4751
                     /gene="gcvP"
                     /locus_tag="ACHUT9_000672"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFR1347231.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5017..5760
                     /locus_tag="ACHUT9_000673"
     CDS             5017..5760
                     /locus_tag="ACHUT9_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1347232.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5817..7250)
                     /gene="bglA"
                     /locus_tag="ACHUT9_000674"
     CDS             complement(5817..7250)
                     /gene="bglA"
                     /locus_tag="ACHUT9_000674"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFR1347233.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            7295..7606
                     /gene="yqfB"
                     /locus_tag="ACHUT9_000675"
     CDS             7295..7606
                     /gene="yqfB"
                     /locus_tag="ACHUT9_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFR1347234.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7770..8429
                     /locus_tag="ACHUT9_000676"
     CDS             7770..8429
                     /locus_tag="ACHUT9_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFR1347235.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8625..9605)
                     /gene="ygfZ"
                     /locus_tag="ACHUT9_000677"
     CDS             complement(8625..9605)
                     /gene="ygfZ"
                     /locus_tag="ACHUT9_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFR1347236.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9848..10114
                     /gene="sdhE"
                     /locus_tag="ACHUT9_000678"
     CDS             9848..10114
                     /gene="sdhE"
                     /locus_tag="ACHUT9_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFR1347237.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10095..10502
                     /locus_tag="ACHUT9_000679"
     CDS             10095..10502
                     /locus_tag="ACHUT9_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFR1347238.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10542..11063)
                     /gene="fldB"
                     /locus_tag="ACHUT9_000680"
     CDS             complement(10542..11063)
                     /gene="fldB"
                     /locus_tag="ACHUT9_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFR1347239.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11175..12071
                     /gene="xerD"
                     /locus_tag="ACHUT9_000681"
     CDS             11175..12071
                     /gene="xerD"
                     /locus_tag="ACHUT9_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFR1347240.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            12096..12806
                     /gene="dsbC"
                     /locus_tag="ACHUT9_000682"
     CDS             12096..12806
                     /gene="dsbC"
                     /locus_tag="ACHUT9_000682"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFR1347241.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12812..14545
                     /gene="recJ"
                     /locus_tag="ACHUT9_000683"
     CDS             12812..14545
                     /gene="recJ"
                     /locus_tag="ACHUT9_000683"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFR1347242.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14636..15734
                     /gene="prfB"
                     /locus_tag="ACHUT9_000684"
     CDS             join(14636..14710,14712..15734)
                     /gene="prfB"
                     /locus_tag="ACHUT9_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFR1347243.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15744..17261
                     /gene="lysS"
                     /locus_tag="ACHUT9_000685"
     CDS             15744..17261
                     /gene="lysS"
                     /locus_tag="ACHUT9_000685"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1347244.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17304..17852)
                     /gene="idi"
                     /locus_tag="ACHUT9_000686"
     CDS             complement(17304..17852)
                     /gene="idi"
                     /locus_tag="ACHUT9_000686"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFR1347245.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            17907..17978
                     /locus_tag="ACHUT9_000687"
     CDS             17907..17978
                     /locus_tag="ACHUT9_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347246.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17975..18100)
                     /gene="yqfG"
                     /locus_tag="ACHUT9_000688"
     CDS             complement(17975..18100)
                     /gene="yqfG"
                     /locus_tag="ACHUT9_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFR1347247.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(18102..19180)
                     /locus_tag="ACHUT9_000689"
                     /pseudo
     CDS             complement(18102..19180)
                     /locus_tag="ACHUT9_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
     gene            19616..21535
                     /gene="ygfT"
                     /locus_tag="ACHUT9_000690"
     CDS             19616..21535
                     /gene="ygfT"
                     /locus_tag="ACHUT9_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFR1347248.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21535..22023
                     /locus_tag="ACHUT9_000691"
     CDS             21535..22023
                     /locus_tag="ACHUT9_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFR1347249.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22092..22442)
                     /gene="ghxQ"
                     /locus_tag="ACHUT9_000692"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             complement(22092..22442)
                     /gene="ghxQ"
                     /locus_tag="ACHUT9_000692"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(22478..22792)
                     /locus_tag="ACHUT9_000693"
     CDS             complement(22478..22792)
                     /locus_tag="ACHUT9_000693"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1347250.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            complement(22725..25664)
                     /locus_tag="ACHUT9_000694"
     CDS             complement(22725..25664)
                     /locus_tag="ACHUT9_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFR1347251.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            complement(25661..26440)
                     /gene="ygfM"
                     /locus_tag="ACHUT9_000695"
     CDS             complement(25661..26440)
                     /gene="ygfM"
                     /locus_tag="ACHUT9_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFR1347252.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(26491..27819)
                     /gene="ssnA"
                     /locus_tag="ACHUT9_000696"
     CDS             complement(26491..27819)
                     /gene="ssnA"
                     /locus_tag="ACHUT9_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFR1347253.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(27822..30920)
                     /gene="ygfK"
                     /locus_tag="ACHUT9_000697"
     CDS             complement(27822..30920)
                     /gene="ygfK"
                     /locus_tag="ACHUT9_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFR1347254.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSS
                     TPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(31242..31820)
                     /gene="mocA"
                     /locus_tag="ACHUT9_000698"
     CDS             complement(31242..31820)
                     /gene="mocA"
                     /locus_tag="ACHUT9_000698"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFR1347255.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            31923..32693
                     /gene="yqeC"
                     /locus_tag="ACHUT9_000699"
     CDS             31923..32693
                     /gene="yqeC"
                     /locus_tag="ACHUT9_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFR1347256.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            32741..34366
                     /gene="yqeB"
                     /locus_tag="ACHUT9_000700"
     CDS             32741..34366
                     /gene="yqeB"
                     /locus_tag="ACHUT9_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFR1347257.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVEWKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILRVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(34407..35339)
                     /gene="arcC"
                     /locus_tag="ACHUT9_000701"
     CDS             complement(34407..35339)
                     /gene="arcC"
                     /locus_tag="ACHUT9_000701"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1347258.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(35387..36772)
                     /gene="hyuA"
                     /locus_tag="ACHUT9_000702"
     CDS             complement(35387..36772)
                     /gene="hyuA"
                     /locus_tag="ACHUT9_000702"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFR1347259.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(36825..38036)
                     /locus_tag="ACHUT9_000703"
     CDS             complement(36825..38036)
                     /locus_tag="ACHUT9_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFR1347260.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(38094..39290)
                     /gene="dpaL"
                     /locus_tag="ACHUT9_000704"
     CDS             complement(38094..39290)
                     /gene="dpaL"
                     /locus_tag="ACHUT9_000704"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFR1347261.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQHQSLMEKLALDKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(39348..40535)
                     /gene="ygeW"
                     /locus_tag="ACHUT9_000705"
     CDS             complement(39348..40535)
                     /gene="ygeW"
                     /locus_tag="ACHUT9_000705"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFR1347262.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            41014..42792
                     /locus_tag="ACHUT9_000706"
     CDS             41014..42792
                     /locus_tag="ACHUT9_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFR1347263.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTSASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(42832..43311)
                     /gene="xdhC"
                     /locus_tag="ACHUT9_000707"
     CDS             complement(42832..43311)
                     /gene="xdhC"
                     /locus_tag="ACHUT9_000707"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFR1347264.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(43308..44186)
                     /gene="xdhB"
                     /locus_tag="ACHUT9_000708"
     CDS             complement(43308..44186)
                     /gene="xdhB"
                     /locus_tag="ACHUT9_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFR1347265.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(44197..46494)
                     /gene="xdhA"
                     /locus_tag="ACHUT9_000709"
     CDS             complement(44197..46494)
                     /gene="xdhA"
                     /locus_tag="ACHUT9_000709"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFR1347266.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            46909..47664
                     /gene="actS"
                     /locus_tag="ACHUT9_000710"
     CDS             46909..47664
                     /gene="actS"
                     /locus_tag="ACHUT9_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFR1347267.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            47743..47812
                     /locus_tag="ACHUT9_000711"
     tRNA            47743..47812
                     /locus_tag="ACHUT9_000711"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47775..47777,aa:Gly,seq:ccc)
     gene            48017..>49281
                     /locus_tag="ACHUT9_000712"
     CDS             48017..>49281
                     /locus_tag="ACHUT9_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFR1347268.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTLD
                     QIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAK"
BASE COUNT        11957 a        12172 c        13004 g        12148 t
ORIGIN      
        1 tctaatggca tcatctttat atagaaactg gtgtgttttt tggtttttta ttctgtcgcg
       61 attttggcgt tttttaacca taagctaatg tgatgatcaa ttttacctta tggttaacag
      121 tctgtttcgg tggtaagttc aggcaaaaga gaacgattgc gttggggacc gggagtggct
      181 ccgatgctgg gtttcgtggt gataatttca ccatgaaaag ttgtcagccc cgcttattca
      241 atgaggacaa gatggcacaa cagactcctt tgtacgaaca acacacgctt tgcggcgctc
      301 gcatggtgga tttccacggc tggatgatgc cgctgcatta cggttcgcaa atcgacgaac
      361 atcatgcggt acgtaccgat gccggaatgt ttgatgtgtc acatatgacc atcgtcgatc
      421 tccgcggcag ccgcacccgg gagtttctgc gttatctgct ggcgaacgat gtggcgaagc
      481 tcaccaaaag cggcaaagcc ctttactcgg ggatgttgaa tgcctctggc ggtgtgatag
      541 atgacctcat cgtctactac tttactgaag atttcttccg cctcgttgtt aactccgcca
      601 cccgcgaaaa agacctctcc tggattaccc aacacgctga acctttcggc atcgaaatta
      661 ccgttcgtga tgacctttcc atgattgccg tgcaagggcc gaatgcgcag gcaaaagctg
      721 ccacactgtt taatgacgcc cagcgtcagg cggtggaagg gatgaaaccg ttctttggcg
      781 tgcaggcggg cgatctgttt attgccacca ctggttatac cggtgaagcg ggctatgaaa
      841 ttgcgctgcc caatgaaaaa gcggccgatt tctggcgtgc gctggtggaa gcgggtgtta
      901 agccatgtgg cttgggcgcg cgtgacacgc tgcgtctgga agcgggcatg aatctttatg
      961 gtcaggagat ggacgaaact atttctcctt tagccgccaa catgggctgg actatcgcct
     1021 gggaaccggc agatcgtgac tttatcggtc gtgaagccct ggaagtgcag cgtgagcatg
     1081 gtacagaaaa actggttggt ctggtgatga ccgaaaaagg cgtgctgcgt aatgaactgc
     1141 cggtacgctt taccgatgcg cagggcaacc agcatgaagg cattatcacc agcggtactt
     1201 tctccccgac gctgggttac agcattgcgc tggcgcgcgt gctggaaggt attggcgaaa
     1261 cggcgattgt gcaaattcgc aaccgtgaaa tgtcggttaa agtgacaaaa cctgtttttg
     1321 tgcgtaacgg caaagccgtc gcgtgattta cttttttgga gattgattga tgagcaacgt
     1381 accagcagaa ctgaaataca gcaaagaaca cgaatggctg cgtaaagaag ccgacggcac
     1441 ttacaccgtt ggtattaccg aacatgctca ggagctgtta ggcgatatgg tgtttgttga
     1501 cctgccggaa gtgggcgcaa cggttagcgc gggcgatgac tgcgcggttg ccgaatcagt
     1561 aaaagcggcg tcagacattt atgcgccagt aagcggtgaa atcgtggcgg taaacgacgc
     1621 actgagcgat tccccggaac tggtgaacag cgaaccgtat gcaggtggct ggatctttaa
     1681 aatcaaagcc agcgatgaaa gcgaactgga atcactgctg gatgcgaccg catacgaagc
     1741 attgttagaa gacgagtaac ggctttattc ctcttctgcg ggagaggatc agggtgagga
     1801 aaatttatgc ctcaccctca ctctcttcgt aaggagagag gttcacaatt cactgcacgt
     1861 ttcaggaacc atcgctcatg acacagacgt taagccagct tgaaaacagc ggcgctttta
     1921 ttgaacgcca tatcggaccg gacgccgcgc aacagcaaga aatgctgaat gccgttggcg
     1981 cacaatcgtt aaacgcgctg accggccaga ttgtaccgaa agatattcag cttgcgaccc
     2041 cgccgcaggt tggcgcaccg gcgaccgaat acgccgcact ggcagaactc aaggctattg
     2101 ccagtcgcaa taaacgcttc acgtcttaca tcggcatggg ttacaccgcc gtgcagctac
     2161 cgccggttat cctgcgtaac atgctggaaa atccgggctg gtataccgcg tacactccgt
     2221 atcaacctga agtctcccag ggccgccttg aagcactgct caacttccag caggtaacgc
     2281 tggatttgac tggactggat atggcctctg cttctcttct ggacgaggcc accgctgccg
     2341 ccgaagcaat ggcgatggcg aaacgcgtca gcaaactgaa aaatgccaac cgcttcttcg
     2401 tggcttccga tgtgcatccg caaacgctgg atgtggtccg tactcgtgcc gaaacctttg
     2461 gttttgaagt gattgtcgat gacgcgcaaa aagtgctcga ccatcaggac gtcttcggcg
     2521 tgctgttaca gcaggtaggc actaccggtg aaattcacga ctacactgcg cttattagcg
     2581 aactgaaatc acgcaaaatt gtggtcagcg ttgccgccga tattatggcg ctggtgctgt
     2641 taactgcgcc gggtaaacag ggcgcggata ttgtttttgg ttcggcgcaa cgcttcggcg
     2701 tgccgatggg ctacggtggc ccacacgcgg cattctttgc ggcgaaagat gaatacaaac
     2761 gctcaatgcc gggccgtatt atcggtgtat cgaaagatgc agctggcaat accgcgctgc
     2821 gcatggcgat gcagactcgc gagcaacata ttcgtcgtga gaaagcgaac tccaacattt
     2881 gtacttccca ggtactgctg gcaaacatcg ccagcctgta tgccgtttat cacggcccgg
     2941 ttggcctgaa acgtatcgct aaccgcattc accgtctgac cgatattctg gcggcgggcc
     3001 tgcaacaaaa aggtctgaaa ctgcgccatg cgcactattt cgacaccttg tgtgtggaag
     3061 tggccgacaa agcgggcgta ctgacgcgtg ccgaagcggc tgaaatcaac ctgcgtagcg
     3121 atatcctgaa cgccgtgggg atcacccttg atgaaaccac cacgcgtgaa aacgtaatgc
     3181 agcttttcag cgtgctgctg ggcgataacc acgggctgga gatcgacacg ctggacaaag
     3241 acgtggctca cgacagccgc tctatccagc ctgcgatgct gcgcgacgac gaaatcctca
     3301 cccatccggt gtttaatcgc taccacagcg aaaccgaaat gatgcgctat atgcactcgc
     3361 tggagcgtaa agatctggcg ctgaatcagg cgatgatccc gctgggttcc tgcaccatga
     3421 aactgaacgc cgccgccgag atgatcccaa tcacctggcc ggaatttgcc gaactgcacc
     3481 cgttttgccc gccggaacag gctgaaggtt atcagcagat gattgcgcag ctggctgact
     3541 ggctggtgaa actgaccggt tacgacgccg tttgtatgca gccgaactcc ggcgcacagg
     3601 gcgaatacgc gggcctgctg gcgattcgtc attatcatga aagccgcaac gaagggcatc
     3661 gcgatatctg cctgatcccg gcttctgcgc acggaactaa ccccgcttct gcacatatgg
     3721 caggaatgca ggtggtggtt gtggcgtgtg ataaaaacgg caacatcgat ctgactgatc
     3781 tgcgcgcgaa agcggaacag gcgggcgata acctctcctg tatcatggtg acttatcctt
     3841 ctacccacgg cgtgtatgaa gaaacgatcc gtgaagtgtg tgaagtcgtg catcagttcg
     3901 gcggtcaggt ttaccttgat ggcgcgaaca tgaacgccca ggttggcatc acctcgccgg
     3961 gctttattgg cgcggacgtt tcgcacctca acctgcataa aactttctgc attccgcacg
     4021 gcggtggtgg tccgggtatg ggaccgatcg gtgtgaaagc gcatctggca ccgtttgtac
     4081 cgggtcatag cgtggtgcaa atcgaaggca tgttaacccg tcagggcgca gtttctgcgg
     4141 caccgttcgg tagcgcctct atcctgccaa tcagctggat gtacatccgc atgatgggcg
     4201 cagaagggct gaaaaaagca agccaggtgg caatcctcaa cgccaactat attgccagcc
     4261 gcctgcagga tgccttcccg gtgctgtata ccggtcgcga cggtcgcgtg gcgcacgaat
     4321 gtattctcga tattcgcccg ctgaaagaag aaaccggcat cagcgagctg gatattgcca
     4381 agcgcctgat cgactacggt ttccacgcgc cgacgatgtc gttcccggtg gcgggtacgc
     4441 tgatggttga accgactgaa tctgaaagca aagtggaact ggatcgcttt atcgacgcga
     4501 tgctggctat ccgcgcagaa attgaccagg tgaaagccgc tgtctggccg ctggaagata
     4561 acccgctggt gaacgcgccg cacattcaga gcgaactggt cgccgagtgg gcgcatccgt
     4621 acagccgtga agttgcggta ttcccggcag gcgtggcaga caaatactgg ccgacggtga
     4681 aacgtctgga tgatgtttac ggcgaccgta acctgttctg ctcctgcgta ccgattagcg
     4741 aataccagta attcactgat tcgactattt tctaaaggcg cttcggcgcc tttttagtca
     4801 gatgacaaag tacaaaagtg ctcagacagt cccctcgccc ctttggggag agggttaggg
     4861 tgaggggaac aggccggcac tggcgcgaat atttaccctc accccggccc tctccctgaa
     4921 agggcgaggg gaaaagcgtg ccaacattga agattgagcc agtttgttag caatctcaaa
     4981 gatacgtcaa cgaattaatt tttctcggaa aaacaaatgg ctatagcact tgtgaccgat
     5041 ggcagtcgcg gcatcgggcg ggcaactgca ttactgttgg cgaaagaaga gtatacggtg
     5101 gcggttaatt atcaacaaaa tctccatgcg gcgcaggaag tggtgaactt aatcacgcaa
     5161 gccggtggca aggcattcgt gctccaggcg gatatcagcg acgaaaatca ggtcatcgca
     5221 atgtttacag caatcgatca gcacgatgaa ccgctagcag cgctggtcaa taacgccggg
     5281 atcttgttta cccagtgcac cgttgaaaac cttaccgcag agcgaatcaa ccgagtactt
     5341 tccaccaaca tgacgggata ttttctctgc tgccgcgagg cggtaaaacg catggcgctt
     5401 aaaaatggtg gcagtggcgg cgctatcgtc aatgtctctt cggtggcctc acggttgggt
     5461 tcgccagggg aatatgttga ttacgcggca tcgaaagggg cgattgatac gttaaccacc
     5521 ggactatcgc tggaagtcgc cgcgcagggg gtccgcgtta actgcgtgcg gccagggttt
     5581 atttttaccg aaatgcacgc cagcggcggc gagcctggac gcgtcgatcg cgttaagtcg
     5641 aacatcccca tgcagcgtgg tggacaggca gaagaggtcg cgcaggccat tgtctggcta
     5701 ctaagtgata aagcctctta cgtcacggga agttttatcg atttggcggg cgggaaataa
     5761 aacagggaag ttgtctgacc ggatgcaaca agtattgcat ccggtacttc atcgacttaa
     5821 agcttctcgc cgttgctggt aatcacctct ttgtaccagt taaagctctt cttacgtgaa
     5881 cgcgacatat cgccagtacc gtcgtcatgt ttattcacat agataaagcc gtagcgtttg
     5941 ctgtactgcc cggtggtgaa cgatacgcag tcgatgcagc cccacggtgt gtagcccatc
     6001 agatccacgc catcgtaagt caccgctttt ttcatctctt cgatatgggc gcgcaggtag
     6061 tcaatgcggt agtcgtcgtt gatgctgcca tcttcttcca ctttgtcgta agcgccaaaa
     6121 ccgttttcga caataaacag cggcctctga taacgctcat acagttcgca aagggcatag
     6181 cgcagaccta ctggatcaat ctgccagccc cagtcagacg ctttaacata cgggttcggt
     6241 acgctgcctt cgaaaccaga gatcgcatcg ccggtgccgc cttcggcctt cactgcgttg
     6301 gtcatgtaat agctgaaacc aagataatcg caggtgcctt cgcgcagtac atcgagatcg
     6361 ccgtcttcca ttttgatgtt aaatccgcga cgctcccact cgttcaacac ataggacggg
     6421 taatagccgc gtagctgcac atcggtaaag acgtagcgtt cgcgcatcga ctcctgagcg
     6481 aacatcacat cgtccgggtt acagcagtag ggatagagcg gcaccatcgc cagcatacag
     6541 ccgactttca tctccgggtt aatgcgacgc gcagctttca ccgccagggc gctggcgaca
     6601 aactggtgat gcagcacctg atacatcgtc tcttccgggt tctcatgctc ggtataaacc
     6661 acgccggagc agcagtaacc gaacagcggt gcacgccagt tacgctggtt gttaatttcg
     6721 ttgaaggtca tccagtattt gactttatgc ttatagcgtt caaacaccac ttccgcgaaa
     6781 cggacaaaga aatcaaccac tttacggttg gtccagctac cgtattgctg caccagatgc
     6841 agcggcattt caaagtggga gagggtgatc accggttcga tgttgtattt cagcagttca
     6901 tcgaacatat catcgtagaa cttcagccct tcttcgtttg gctgagcttc atcgcctttt
     6961 ggaaaaatgc gcgtccaggc tatggaagtc cgaaaacatt tgaagcccat ttcggcaaat
     7021 agcttgatgt cttccttgta gtgaccataa aaatcaacgg cttcatggtt tggatagtat
     7081 ttgcctggca agacttcttt ggtgatttcg cgcggcacgc cgtgtgcgcc accggtcaga
     7141 acgtcacaaa tgctcggccc tttgccgcct ttgttccagc cgccttcgac ctgatgagcg
     7201 gcaactgcgc cgccccataa gaaatctttc tgtaaggtga gttttttcac tatcataggc
     7261 tccagatgag tcaattctta caggaaatag taccatgcag ccaaacgaca tcactttttt
     7321 tcaacgtttc caggatgaca ttctggctgg gcgtaaaacc atcaccatcc gcgacgagtc
     7381 tgaatcgcac ttcaaaacgg gtgatgtgct tcgtgtcggg cgttttgaag atgacggtta
     7441 tttttgcacg attgaagtca ccgcaacctc aaccgtaacg ctggatacgc tgacggaaaa
     7501 acatgcagag caggaaaata tgaccctgac tgaactgata aaggtcattg ccgacatcta
     7561 tcccggtcag acacagtttt atgtgattga atttaaatgt ctttaaattg aactcacgcc
     7621 tgttagttga aatttcgcat ttaatttcat gatttttagg agattagtct caaatcagaa
     7681 tttattttag ctaacaggtg ttcactggaa ctattctcag ttacgctgga gaggtataca
     7741 tgtgcgtgta cgtttctccg gagtaagtta tggttcagaa gcccctcatt aagcagggat
     7801 attcgctggc agaggaaatt gccaacagcg tcagtcacgg cattgggttg gtgtttggta
     7861 tcgttgggct ggtgttgcta ctggttcagg cggtggatct caatgccagc gccacagcga
     7921 taaccagtta cagcctctat ggcggcagta tgatcctgct gttcctcgct tcgacgctct
     7981 atcacgccat tcctcatcaa cgggcaaaaa tgtggctgaa gaaatttgac cattgcgcta
     8041 tttacctgtt gattgccgga acctacacgc cgtttttgct ggtggggctg gattctccgt
     8101 tagcgcgcgg gttgatgatt gttatctgga gcctggcatt gctgggtatt ctgtttaaac
     8161 tgaccatcgc gcaccgattc aaaattttat ctctggtgac ctatctggcg atgggctggc
     8221 tgtcgctggt ggtaatttat gaaatggcag ttaagctcgc ggcgggcagc gttaccttat
     8281 tggcggtagg cggcgtggtt tattcgctcg gagtgatttt ctatgtctgc aaacgcattc
     8341 cctacaatca tgccatctgg cacggcttcg tgctcggcgg cagtgtgtgc cactttctgg
     8401 cgatctattt gtatattggg caggcgtaat gggtatgcct ttgtaggcct gaaaagacgc
     8461 gtcagcgtcg catcaggcat cggagcacaa ctgccggatt cggcgtgaac gccttatccg
     8521 atctacgggc ctcgtccctg ttgtgataaa ggcatcaaat cccagacatg ataagacgcg
     8581 tcagcgtcgc atcaggcgat aaatgcctga tgcgataaag agatttactc ttcgagcgaa
     8641 tacggcagcg gctcaatatg caatgtattc gcatcgtcgc gtacgcggaa gatgctatcc
     8701 ggttccatat cgttattcat gacgacctgt accacgacct gaccatcttc cagttttacc
     8761 gcagccagca ccgtaccggt acgacgccag ttctcgccca ttttcagctc taagtcttca
     8821 ccagcttccg gcagtcggct ggcgctacct gccagcaacc agagcgcacg tttattggca
     8881 ccacggaatt ttgctcgcgc caccatctct tgtccggtat aacagccttt cttaaagctg
     8941 ataccgccca gcgcctggag attggtcgcc tgtgggataa actgcccgct gttggcggca
     9001 tcaatcaccg ggaaacccgc ttcaatgttt aatgccagcc actgttggct attgttcagt
     9061 tccgcttcac cgcgcagttt atcggtcagc atattagcag tagcttcatc ggttacgatc
     9121 aggaaacgtt ctgccgggta ttcaaaccat agcaaagtgg tcgcgccttc tttaactacc
     9181 tgtttttctt tcgaaggcag ttcactaaag atatttgcca gcgcggcgcg cgcctgaaaa
     9241 ccggcaacac caagcagcac atgctcgtcg tctggcgcga tggtcacttt agagaacacc
     9301 gcatattttt tcagttcagt cagctgcggt tcacgcacgc tgcgccgttc aatccacgca
     9361 aagccatcgc cgtcgcggaa cagacgtaaa ttgctccaca ttttgccttt ggcgtcgcaa
     9421 tgggcggcga gcaggtgctg atcttctgcc atctggctga catctgctgt cacctgaccc
     9481 tgcatatatt tttcgctgtc cgcgccagta atggtggcaa gcgcccagtc atcaagcgtc
     9541 atcagcgtca gcggtaaacg ggcagaagcc gtaggctgac ggggaggaaa aggtgtaaaa
     9601 gccataatag agtcctgagt tgcttaacgg cgtattaatg actaatggta aaagagctat
     9661 gaggcaatgc aagtgctttc actacgcgat ctgtgccttc tgcgaggcga cttccaaaaa
     9721 aatgaagcca ttgaattgtt gtgaattaat ttcgtgatcg cgatggtgtt tactgatatt
     9781 cccgtaaaag aaggcgaaaa acacgttaca atagcggggt actggatttt cgtgagacag
     9841 gaagaagatg gacattaaca acaaagcccg cattcattgg gcatgccgcc gtggtatgcg
     9901 cgaactcgat atttcaatca tgccgttttt cgaacatgag tacgacagct taagcgatga
     9961 cgaaaaacgc atctttattc gtctgctgga atgtgacgat ccggacctgt ttaactggct
    10021 gatgaatcac ggtaaaccgg ccgatgcaga actggaaatg atggtccgac tcatccagac
    10081 acggaaccgg gaacgtggtc ctgtggcaat ctgatttgcg cgtctcctgg cgcgcacagt
    10141 ggctttcctt gctgattcat gggctggttg ccgctgttat tttactcatg ccctggccac
    10201 tcagttacac cccgttatgg atggtgttac tttcgctggt ggtgtttgat tgcgttcgca
    10261 gccagcggcg tattaatgct cgccaggggg aaattcgctt gttgatggac gggcgtttgc
    10321 gttggcaagg gcaggagtgg agcatcgtca aagcaccgtg gatgattaag agcggcatga
    10381 tgctgcgttt acgttctgat ggcggtaaac ggcaacattt atggctggca gccgacagca
    10441 tggacgaagc cgaatggcgg gatttacggc ggattttgtt gcaacaagag acgcaaagat
    10501 aaagatgacg gcaaagcata tgcgctaagc cgtcagtgag atcaggcgta atgctctgcc
    10561 atttcgttga ggatttgctc gcaccagctc tgaatacgct cgtcgctaag gtcatactgg
    10621 ttagtttcat ccagcgccag acccacgaac agttgcccgt cagcaatcac cggtttcggg
    10681 ctggtaaatt catatccttc cgttggccag tagccgacga acttcacgcc tttggtcgag
    10741 agtttgtcat gcagcatacc gagcgcatcg aggaaccact cgccgtatcc cagttgatcg
    10801 ccaagcccat acagcgcaac aattttacct tcaaggttca ggtcgtcgag ctgatcccag
    10861 acggcttccc agtcttcctg gatttcacca aaatcccagg tcgggatacc cagaatgagc
    10921 acatcgtact gctccattaa tttcggggag tcgtccttga ggttatgtaa ggtcaccagt
    10981 tctgggccga taatatcgcg gattttttct gccgccattt cggtgtaaca ggtgctggaa
    11041 ccgtaaaaaa gacccatatt cataacgtaa gcttctcgat tctgctgtgg cttttgcagc
    11101 tagtgtatca gaatcgcttt agtcacggca caataaggca taatggatca aatgagtgac
    11161 cataaggggc gcaagtgaaa caggatctgg cacgcatcga gcagtttctt gatgctctgt
    11221 ggctggaaaa aaatctggct gaaaatacgt tgaacgctta ccgtcgcgat ctgtcaatga
    11281 tggtggagtg gttgcatcac cgcgggttga cgctggcgac ggcgcaaagt gacgatttgc
    11341 aggcattact ggcagaacgg ctggagggcg ggtataaagc caccagctca gcgcgtttgc
    11401 tgagtgcagt gcgccgattg ttccagtatc tttatcgcga aaagtttcgt gaagacgatc
    11461 ccagtgcgca tctcgcttca ccgaaattgc cccagcgttt gccaaaagat ttaagcgaag
    11521 cgcaggtcga acgtttatta caggcgccat taattgatca gccactggag ctacgcgata
    11581 aagccatgct tgaagtgttg tatgctaccg ggctgcgtgt ctctgaactg gtcgggctga
    11641 caatgagtga tatcagcctg cgtcagggcg tagtgcgggt cattggtaaa ggcaacaaag
    11701 agcgcctggt accgttaggt gaagaggcgg tttactggct ggaaacctat ctggaacatg
    11761 ggcgtccgtg gctgctgaat ggtgtgtcaa ttgacgtgtt gtttcccagc cagcgtgcgc
    11821 agcagatgac gcgacagacc ttctggcacc gcattaaaca ttatgccgtg ctggcgggta
    11881 tcgacagcga aaagctgtca ccgcatgtgt tgcgtcacgc ttttgccact catttattaa
    11941 atcatggtgc ggatttacgc gtggtgcaga tgctactggg ccacagcgat ctttccacca
    12001 cgcaaattta tacgcatgtc gctaccgagc gtctgcggca acttcatcaa cagcatcacc
    12061 cgcgggcgtg atgtctgaaa agaacgggaa gatttatgaa gaaaggtttt atgttgttta
    12121 ctttgttagc ggcgttttca ggttttgctc aggctgatga cgcggcaatt caacaaacgt
    12181 tagccaaaat gggcatcaaa agcagcgata ttcagcccgc gcccgtagct ggcatgaaga
    12241 cagttctgac taacagcggc gtgttgtaca tcaccgatga tggtaaacat atcattcagg
    12301 ggccaatgta tgacgttagc ggcacggctc cggtcaatgt caccaataag atgctgttaa
    12361 agcagttgaa tgcgcttgaa aaagagatga tcgtttataa agcgctgcag gaaaaacacg
    12421 tcatcaccgt gtttactgat attacctgtg gttactgcca caaactgcat gagcaaatgg
    12481 cagactataa cgcgctgggg atcaccgtgc gttatcttgc tttcccgcgc caggggctgg
    12541 acagcgatgc agagaaagaa atgaaagcta tctggtgtgc gaaagataaa aacaaagcgt
    12601 ttgatgatgt gatggcaggt aaaagcgtcg caccagccag ctgcgacgtg gatattgccg
    12661 accattacgc acttggcgtc cagcttggcg ttagcggtac tccggcagtt gtgctgagca
    12721 atggcacact tgttccgggt taccagccgc cgaaagagat gaaagaattc ctcgacgaac
    12781 accaaaaaat gaccagcggt aaataattcg cgtgaaacaa cagatacaac ttcgtcgccg
    12841 tgcagtcgat gaaacggcag acttgcccgc tgaattgcct cccttgctgc gccgtttata
    12901 cgccagccgg ggcgtgcgca gtgcgcaaga actggaacgc agtgttaaag ggatgctgcc
    12961 ctggcagcaa ctgagcggcg tcgaaaaggc cgttgagatc ctttacaacg cctttcgcga
    13021 aggaacgcgg attattgtgg tcggtgattt cgacgccgac ggtgccacca gcacggctct
    13081 aagcgtgctg gcgatgcgct cgcttggttg cagcaatatc gactacctgg ttccaaaccg
    13141 tttcgaagac ggttacggct taagcccgga agtggtcgat caggcccatg cccgtggtgc
    13201 gcagttaatt gtcacggtgg ataacggtat ttcctcccat gcgggggttg aacacgctcg
    13261 ctcgttgggc attccggtta ttgttaccga tcaccatttg ccgggcgaaa cattacccgc
    13321 ggcggaagcg atcattaacc ctaacttgcg cgactgtaat ttcccgtcga aatcactggc
    13381 aggcgtgggt gtggcgtttt atctgatgct ggcgctgcgc acctttttgc gcgatcaggg
    13441 ctggtttgat gagcgcggca tcgcaattcc taacctggca gaactgctgg atctggtagc
    13501 gctggggaca gtggcggacg tcgtaccgct ggacgctaat aatcgcattc tgacctggca
    13561 ggggatgagt cgcatccgtg ccggaaagtg ccgtccgggg attaaagcgc tgcttgaagt
    13621 ggcaaaccgt gatgcacaaa aactcgccgc cagcgattta ggttttgcgc tggggccacg
    13681 tctcaatgct gccggacgac tggacgatat gtccgtcggt gtggcgctct tgctgtgcga
    13741 caacatcggc gaagcgcgcg tgctggcaaa tgaactcgat gcgctaaacc agacgcgaaa
    13801 agagatcgaa caaggaatgc aggttgaagc cctgaccctg tgcgagaaac tggagcgcag
    13861 ccgtgacacg ctacccggcg ggctggcaat gtatcacccc gaatggcatc agggggttgt
    13921 cggtattctg gcttcccgta ttaaagagcg ttttcaccgt ccggttatcg cctttgcgcc
    13981 cgcgggtgat ggtacgctga aaggttctgg tcgctccatt caggggctgc atatgcgtga
    14041 tgcgctggag cgattagaca cactctaccc tggcatgatg ctgaagtttg gcggtcatgc
    14101 gatggcggcg ggtttgtcgc tggaagcgga taaattcgaa ctctttcaac aacggtttgg
    14161 cgaactggtt actgagtggc tggacccttc gctattgcaa ggcgaagtgg tgtcagacgg
    14221 tccgttaagc ccggccgaaa tgaccatgga agtggcgcag ctgctgcgcg atgctggccc
    14281 gtgggggcag atgttcccgg agccgctgtt tgacggtcat ttccggctgc tgcaacagcg
    14341 gctggtgggc gaacgtcatt tgaaagtcat ggtcgaaccg gtcggcggcg gtccgctgct
    14401 ggatggtatt gcttttaatg tcgataccgc cctctggccg gataacggcg tgcgcgaagt
    14461 gcaactggct tacaagctcg atatcaacga gtttcgcggc aaccgcagcc tgcaaattat
    14521 catcgacaat atctggccaa tttagcgtca tcttctctat aaaaaagagc gtggattggg
    14581 tacaatcccg ctcttatcac cgcattttga ctagctcaat aaaagaaatc agaccatgtt
    14641 tgaaattaat ccggtaaata atcgcattca ggacctcacg gaacgctccg acgttcttag
    14701 ggggtatctt tgactacgac gccaagaaag agcgtctgga agaagtaaac gccgagctgg
    14761 aacagccgga tgtctggaat gaacccgaac gcgcacaggc gctgggtaaa gagcgttcct
    14821 ccctcgaagc cgttgtcgac accctcgacc aaatgaaaca ggggctggaa gatgtttctg
    14881 gtctgctgga actggctgta gaagctgacg acgaagaaac ctttaacgaa gccgttgctg
    14941 aactcgacgc cctggaagaa aaactggcgc agcttgagtt ccgccgtatg ttctctggcg
    15001 aatatgacag cgccgactgc tacctcgata ttcaggcggg gtctggcggt acggaagcac
    15061 aggactgggc gagcatgctt gagcgtatgt atctgcgctg ggcagaatcg cgtggtttca
    15121 aaactgaaat catcgaagag tcggaaggtg aagtggcggg tattaaatcc gtgacgatca
    15181 aaatctccgg cgattacgct tacggctggc tgcgtacaga aactggcgtt caccgcctgg
    15241 tgcgtaagag cccgtttgac tccggcggtc gtcgccacac gtcgttcagc tctgcgtttg
    15301 tttatccgga agttgatgat gatattgata tcgaaatcaa cccggcggat ctgcgcattg
    15361 acgtttatcg cgcgtccggc gcgggcggtc agcacgttaa ccgtaccgaa tctgcggtgc
    15421 gtattaccca catcccgacc gggatcgtga cccagtgcca gaacgaccgt tcccagcaca
    15481 agaacaaaga tcaggccatg aagcagatga aagcgaagct ttatgaactg gagatgcaga
    15541 agaaaaatgc cgagaaacag gcgatggaag ataacaaatc tgacatcggc tggggcagcc
    15601 agattcgttc ttatgtcctt gatgactccc gcattaaaga tctgcgtacc ggggtagaaa
    15661 ctcgcaacac gcaggccgtg ctggacggca gcctggatca atttatcgaa gcaagtttga
    15721 aagcagggtt atgaggaacc aacatgtctg aacaacacgc acagggcgct gacgcggtag
    15781 tcgatcttaa caatgaactg aaaacgcgtc gtgagaagct ggcgaacctg cgcgagcagg
    15841 ggattgcctt cccgaacgat ttccgtcgcg atcatacctc tgaccaattg cacgcagaat
    15901 tcgacggtaa agagaacgaa gaactggaag cgctgaacat cgaagtcgcc gttgctggcc
    15961 gcatgatgac ccgccgtatt atgggtaaag cgtctttcgt taccctgcag gatgtgggtg
    16021 gccgcattca gctgtacgtt gcccgtgacg atctgccgga aggcgtttac aacgagcagt
    16081 tcaaaaaatg ggacctcggc gacatcctcg gcgcgaaagg taaactgttc aaaaccaaaa
    16141 ccggcgaact gtctatccac tgtaccgagc tgcgtctgct gaccaaagca ctgcgtccgc
    16201 tgccggataa attccacggc ttgcaggatc aggaagcgcg ctatcgtcag cgttatctgg
    16261 atctcatctc taacgatgaa tcccgtaaca cctttaaagt acgctcgcag atcctctctg
    16321 gtattcgcca gttcatggtg aaccgcggct ttatggaagt tgaaacgccg atgatgcagg
    16381 tgatcccagg cggtgccgct gcgcgtccgt ttatcaccca ccataacgcg ctggatctcg
    16441 acatgtacct gcgtatcgcg ccggaactgt acctcaagcg tctggtggtc ggtggcttcg
    16501 agcgtgtatt cgaaatcaac cgtaacttcc gtaacgaagg tatttccgta cgtcataacc
    16561 cagagttcac catgatggaa ctctacatgg cttacgcaga ttacaaagat ctgatcgagc
    16621 tgaccgaatc gctgttccgt actctggcac aggatattct cggtaagacg gaagtgacct
    16681 acggcgacgt gacgctggac ttcggtaaac cgttcgaaaa actgaccatg cgtgaagcga
    16741 tcaagaaata tcgcccggaa accgacatgg cggatctgga taacttcgac tctgcgaaag
    16801 cgattgctga atctatcggt atccacgttg agaagagctg gggtctgggc cgtatcgtta
    16861 ccgagatctt cgaagaagtg gcagaagcac atctgattca gccgaccttc attactgaat
    16921 atccggcaga agtttctccg ctggcgcgtc gtaacgacat taacccggaa atcacagacc
    16981 gctttgagtt cttcatcggt ggtcgtgaaa tcggtaacgg ctttagcgag ctgaatgacg
    17041 cggaagatca ggcgcaacgc ttcctggatc aggttgccgc gaaagacgca ggtgacgacg
    17101 aagcgatgtt ctacgatgaa gattacgtta ccgcactgga acatggctta ccgccgacag
    17161 caggtctggg gattggcatc gaccgtatgg taatgctgtt caccaacagc cataccatcc
    17221 gcgacgttat tctgttcccg gcgatgcgtc cggtaaaata agcattacgt tatgctcaca
    17281 accccggcaa atgtcggggt tttttaattg tgctgcgcga aagcagacaa caattttctt
    17341 gcttcactat tggctgcctg catcaccatc cacggactga acgcccacgg tgtggcatca
    17401 ataccgtgta atacatctgc taaatcacac cattgataat ccatcacttc atcatcgttg
    17461 atctgtaacg cactggtggt gcgtgcggca aataccggac acacttcatt ttccacaatg
    17521 ccattcggat cggtggcgcg atagcgaaag tcaggataga tagattcagg aggcgtaatt
    17581 tccacgccaa gctcataacg gcaacggcgg atcaccgcgt cttcgttgct ttctcccagt
    17641 tgtgggtgcc cacaaaccga gttagtccac acgccaggcc acgctttttt gctcagtgcg
    17701 cggcgggtaa ctaataattg tcctttggca ttaaacagcc aactggagaa cgcgagatgt
    17761 aagcgggtgt ctgccgtgtg tgcggcatac ttttccagcg tacccgtggg aactccctgt
    17821 gcattcaata aaatgacgtg ttccgtttgc ataatttctc acatgtaatt ctgatcactc
    17881 atcattatag ataattgaag agtgaaatga ttaaccaagt gagcgtttat cgacaaccgc
    17941 ccgttttgag cggatgccga caggtaaaaa ccatttaaaa aagtagcatc atatatttga
    18001 agcgactttc aatggccatt gcaacacagt cagaaatgac aggaatagag agagtaaaaa
    18061 gcaacagcag actgaatata acgcgcatta aaaaattcat attattctcc atgctcattt
    18121 ttcttcagct tgcgcatcag aagccacata cgtactgtcc tgacttttaa atctttatca
    18181 gagacggatt ccttcaccag gtcagcatga tgctggtagc cattaaagaa gacattcagc
    18241 acaacggcgc taagggttgc gagcataatg ccgctatgca gcaacggttg cagtacggcc
    18301 ggtaacttag aaaagaaatc gtgagagagc gtcggagtca tgccaacgcc gagactgatc
    18361 gccacaatat agaggttgta acggttggtg gtgtagttac agcgcgacag aattcgaatc
    18421 cctgtcgcca gtaccatgcc gaacatcacc agcccagcac cgcccagcac aaattgcgga
    18481 atggaggcga ccagcaccgc catttttggc accatgccaa acaggattaa tataattccc
    18541 gaagaaatac acacccagcg gctataaatc ctttcgcata gcgagtaatg agcaagataa
    18601 aaattaagac agcaaaggag atacctaaat aaacgggatt accatattgc ggattccctt
    18661 tacctccggc ggcccagtca atacccacct gaatgatgct aagcccaata gaagtaataa
    18721 ccacaccggt aaccagtggc gggaataaag gcatcaagcg accaataaat ggcgctaata
    18781 atgtggtgat aaaacccgcg gcgatagtgg caccaaatat ccccagcagg ccgatatccg
    18841 ggttcatccc aatggctatc attggtgtta cagcagcaaa ggtcaccgac ataatcaccg
    18901 gcaggcggat ccccataaag cggccgatac cgatacattg caataatgtg acgatcccgc
    18961 agcaaaagag atccgagcta atgagcatcg caatagcatc ttttgagagg cccagtcggt
    19021 caccaatcat aagaggaaca gcgactgcac ctgcgtacat gacaaggacg tgttgcagac
    19081 cgagtatgat cagctttcct ggtgataata tgcggtcaac ctcatcagtt gggcggtctt
    19141 gcccagaaga tgagggaagt tgggaatcta tggcgctcat cagtgtttct cctttcgccc
    19201 gccagagtaa aaagcaaccg tttacgttaa tgaaactgtc taaacacatg ctcgtggagt
    19261 gccagttttt gcgaggaagg ggagttagag acaggaattg cggaggcaat cacataatcg
    19321 tgcttaaacc acgaaaatga caagagaacc tgacaatgga attatctgta attttctcat
    19381 attgatattt cattaatcat tctggcatgt cgcactctag catttaatcg tttttatctg
    19441 gatagcgctc ttttgatcgg caatgttgaa ataatgaaag cggcatagat tgaggggcaa
    19501 taatttgaac tcagtcgcaa aattataatt aaaacggtca tgctacctaa tatatgcccg
    19561 tttattgcat cgcgaaattt gagaagacat aactcattaa atgaaaggga tgcaaatgaa
    19621 taagtttatc gctgctgaag ctgcggaatg tataggctgt catgcttgtg aaattgcctg
    19681 tgcggtggca cacaatcaag aaaactggcc gctgagtcac agtgactttc gaccgcgtat
    19741 ccacgttgta gggaaaggcc aggctgcgaa tccggtggcc tgccatcact gcaacaatgc
    19801 cccttgtgtt acggcttgtc cggttaatgc tctgactttc cagtccgata gcgtacaact
    19861 ggacgagcaa aaatgtattg gctgtaaaag atgcgcaatc gcttgcccct ttggcgtcgt
    19921 tgagatggtc gatacgattg cgcagaaatg cgacctttgt aaccagcgca gttccggcac
    19981 gcaagcctgt attgaagtct gcccaacaca ggcgttacga ctgatggacg ataaagggtt
    20041 acagtggata aaggtggctc gccagcgcaa aacggcagca ggaaaagcgt catcagacgc
    20101 tcagccatct cgcagtgcag cgttgctccc cgttaactcg cgtaaaggcg cagataaaat
    20161 ttcagcgagt gaacggaaaa cccactttgg cgaaatctat tgcgggctgg atccactgca
    20221 agcgacttat gagagtgacc gctgtgttta ttgtgccgaa aaagctaact gcaactggca
    20281 ttgcccgctg cataacgcta ttccggatta catccgtctg gtacaggaag gaaagattat
    20341 tgaagcggca gaactttgcc accagaccag ttccttacca gaaatctgcg gcagggtatg
    20401 cccacaggac cgtctttgtg aaggcgcatg tactttgaaa gatcactctg gcgcagtctc
    20461 tatcggtaat ctggaacgct acatcaccga taccgcgctg gcgatgggct ggcgtcccga
    20521 tgtcagcaaa gttgttcccc gtatcgaaaa agtggcggtg attggcgctg ggccagcagg
    20581 gttagggtgt gctgatattc tggcgcgcgc gggagttcag gttgatgtct ttgatcgcca
    20641 tccagaaatt ggcggtatgc tgacttttgg cattcctcct ttcaaactcg ataaaacggt
    20701 attaagccag cggcgagaga tattcaccgc aatgggaatc gactttcatc ttaactgtga
    20761 aattggccgc gatattacct ttagcgattt aacttctgaa tatgatgcag ttttcatcgg
    20821 cgtggggact tacgggatga tgcgagcaga tctgccgcat gaagatgcgc ccggtgtcat
    20881 tcaggctcta ccgttcctga ctgcccatac ccgccagctc atgggattgc cggagtctga
    20941 agagtatccg ctgacggacg tggaaggtaa gcgagtcgtg gtattgggcg gtggcgatac
    21001 gacaatggat tgtttgcgga cttccattcg cctcaatgct gccagcgtga cctgcgcgta
    21061 tcgtcgtgat gaagtcagta tgccgggctc gcgcaaagag gtggttaatg cgcgcgagga
    21121 aggtgtcgag tttcagttca atgttcaacc gcaatatatc gcttgtgacg aagatgggcg
    21181 tttaactgcg gtgggcctga ttcgtaccgc catgggtgag ccggggccgg atggtcgccg
    21241 tcgtcctcgt ccggtagcgg gttcagagtt tgaattgccc gccgatgttc tgattatggc
    21301 ctttggtttc caggcgcatg ccatgccgtg gttgcagggc agcggaatta aactcgataa
    21361 atggggcctg attcaaaccg gtgacgtcgg gtatttacct acccagacgc atctgaaaaa
    21421 agtctttgct ggtggtgatg cagttcatgg cgcggatctg gttgtcactg caatggccgc
    21481 aggaaggcag gcggcgcgcg atatgttaac tctgtttgat acgaaggcat cgtgatgaaa
    21541 tcgttaatta tcgttaatcc ggctgactgt attggctgcc gtacttgtga agtagcctgt
    21601 gtggtcgctc acccttcaga acaggagttg aatgccgatg tctttttacc ccggctgaag
    21661 gtacagcgac tggacagcat cagtgcgccg gtgatgtgcc atcagtgtga aaacgcccct
    21721 tgtgttggcg cttgccccgt gggggcgctg acgatgggcg agcaggtggt gcagaccaat
    21781 tctgcccgtt gtattggctg tcagagttgc gtcagtgcgt gcccgtttgg aatgatcacc
    21841 attcagtcgt tgccggggga tacccgacaa caaattgtga aatgcgatct ctgtgaacag
    21901 cgggaagagg ggccagcctg tgttgaatct tgcccgacac aggcgctgca gttgcttact
    21961 gaaagagaac tcaggcgagt ccgccagcag cgtattgttg ccagcggtga gaatcctctg
    22021 tgaaagaaat ctccgcgctg aaaggcgcgg agaatctatt agattgccca accacccgcg
    22081 taaaatgcga ccagtgcgac aggaacgcta atttgttgcc cgagtaccag actgaatgcg
    22141 gtaaatgccg tcaaggaaat cgcacaacca atcgccattg gcaaattggc ctacaacccc
    22201 atcagcaacg agccgaagcc cgcgaccaga caggtggcaa caaacacagc tgcgggagga
    22261 aaacctgctt tgcccagcat tcccggaacg acgataacgg aataaaccat ggccagaaag
    22321 gtcgttaagc cagccagtac ttcctgacga acggtactgc cacgagcggt aattttaaaa
    22381 taattatcca gcgcgccctg tggctttggt gcgtccggtg tttgtaggat gtctccagac
    22441 ataatgatgt cctcacgagg atgttaaagt tttattatta gttgcgttcg tacaccagac
    22501 gaccatcaac gtaggtgcgg tagatcgaac ggtcatcgcc caacgtcatc atcacgaaca
    22561 atttgtcgac taaagaaaca gagttgtcat agcgcagctg ctgtagcgga gtggcggtgg
    22621 gttccatcac cacgaaatca gcctctttgc caggtaaaaa gttgccaatc aaatcgtcaa
    22681 ggcccagaga tttcgctccg ccgagcgtgg ccaggtaaaa cgcttcatat gccgagaggc
    22741 gatagccttg taattgcaac actttgtagg cttcgttcag cgtttgcagc atgttgaaag
    22801 ttgttccggc accgatatcc gttcccatgc ccactttgac tttcttctgc ccagatgccg
    22861 catgcatcgt gaattgcggt agcaatcgcc ggagctgcgc catttacacc gatttccgag
    22921 atcgatttcg ccccgaacgg gccgacttta tcgtcgctcg gcaccagcac agcgcggaaa
    22981 tcacgcggaa tgtcaccaat tttcggtgcg ccgtaactgc gtaaatcacg cgttaacggg
    23041 tgaccttcgg cgtcataaat gatctcttcg ctcatactgt ggccgatagc tcgcagggta
    23101 gcaccgtaga tttgtcccaa tgctaattcc gggttgaccg gtgtaccgca gtccagcaag
    23161 gcgtagaatt tatccaggcg gatttcaccc gtacgcgtgt tgacggcaac ttcagcgaag
    23221 tttgcgccat acgggaaggc gaaatcaggc gtgatataac tgccagttcc caccagtgaa
    23281 ccaaagccga tgccggtttc gcctttatgg gcaatatccc cgaaactaac ttcgcctttc
    23341 ttgccgcgca cgacgcccgg cgttgccagt tgaacatctg ccactgcctc acctaacatt
    23401 tgcgcgccgt ggaacagaat tttctcccgt agattttccg ctgccaaacg cgccgcgtta
    23461 cccgagaagc aagtaccgga cgaggcatat gcgcctttat caaacaacgc atgatcggta
    23521 tcaccggaga taacatgcac gtcctgcggt gggcagtgca gcacttctgc tgccagtttc
    23581 gtcactacag tatccagacc agtaccaata tccgcaccgc cagaatgaac gataaaggtg
    23641 ccgtccgatt ccagtttgat catgcagtta gcctgatcga tatccgggat acccgatttc
    23701 tgcatgataa tggcgacgcc gcgaccgatg tgccagtcac cattttgcgg tttcggtgac
    23761 gaccattgga tcatctcgcg accctgacgc aggatctctt ccagtgcaca gctggcggcg
    23821 gaaggaacgg aggtcggcgc tttaccttca ccgattgcac cgagaatttt cagctcttgc
    23881 ccttcgtgta cccggttacg ttcgataatt tccagttggt cgatctgtaa ctgttcagcc
    23941 agttccgcta atgccatggt gatagcgaag ttacctttcg gtgcgccata accctgataa
    24001 gcaccatttg ggcaaatgtt gctgtagtag gtggtgacct ggaaatcgac gttatcgcac
    24061 ggatataacg gcagcgacag cgccggtccg ttacacggta cggtgagtga gtggttgccg
    24121 taagggccag tgttggcgcg gaaatccatc ttcactgccg tcaggcgacc atcttttttc
    24181 gctcccagtt tgacggtgac tttcgcgacg tgacgagagg tgttagcaat aaactcttct
    24241 tcacgggtgt agcggaatag taccggacgc ccggtcacgc aggttgccca ggcgcacact
    24301 tcttccagca ggatgtcctg tttggaacca aaaccgccgc caactcgctc tttaatgaca
    24361 tgaactttat gctgtttcat gcccacgagg cgcgcgacct ggcggcgtaa gtgccatggt
    24421 acctgggtgg aggcgtggat aaccagacga tcgccgtcca tccgggtaaa gcagatatgt
    24481 gtttcagtcg ggcactgctg tgcctgcgtt gagttatagg ttcgctcaat gatcacatcg
    24541 gcatcggcaa agcctttgtc catatcgcca atatgaccat gaatactggc ggcgatattt
    24601 ttgcgagggc gagaaccgat cgggaagttg atgatcatat gctcgccgcg ctgggctgca
    24661 tggctgttat cgtcttccag agtatctggc gcaccagcaa catacaccac cggttcatcg
    24721 tgcacgacag gcgcatcttc cgccattgct tcgtcgatcg acattaccgg cttaagcact
    24781 tcatattcaa cgtcgatgag cttcagtgct tcgagcgcaa tttcttcact ttctgcgacg
    24841 accgcagcaa cacgatcgcc gacgtgacgc attttcttgc cgaacatacg gcggtcaagc
    24901 ggtgacggtt ccggtgcgct ctgacccccc ggggtatagt agatatcagg gcaattcagg
    24961 tgagtaataa cgtgaacgac gcccggtaag gcttcagctt tgctgacatc cagatgagta
    25021 atcagtgcgt gagcgtgtgg gctacgtaac attttaatga cgcaggcgtc agccgttacg
    25081 cagtcttcaa catagcaggg tttcgcctgc accattttcg cggcatcagt tttaggataa
    25141 tgcttgccaa tgacgtcaag gtcgtcacgg aaagtcggag cgatatcaat ggtggcctgc
    25201 ggattatttt tacgtgcaac cgccagttca atgacctgat agtattgttg ccagcctgca
    25261 tcgcggctga acaaaccaga aagcgcgtcg tcgatctctt cgcgggtagg tgcggcgatg
    25321 cgatcgagaa gatcggtgat aatcagagct gcagctggat cgttataacc agactgcacc
    25381 acgccaacat caaccattgc ctgttgaacc agacttaact cgttccattt acccagagat
    25441 tctgcggtac gaatatctgc cttctctaac tgtgcggcaa taagcaagga cgcgttaacg
    25501 atattaccgt taaagattat tgcgtcagaa ccggcaaacc cgaagccatc atcactgttg
    25561 cgtacagagt gcattcccat gttaaacaac agcttttgca cgttttcgcc tggattaacg
    25621 gttagctcct gaggcgcgcc atttaaagta aagtggatga tcatacggct tcctccccag
    25681 cctgttggca gtcggcatac aggtcggcta ccagaactcc cgtgatatag cgtttatagg
    25741 ccacgctgcc ccgcaaatct tcctgcggga aaatggcgtt ggcgacagcc tgttcaagtg
    25801 cattgccttc cagattttgt ttttcgacat cgtgcagacg cagtgcttta ctggcgacgc
    25861 catccagcgc aattcgcata ccgtcgtggt ctgtcattgc aacggctgcc gtcacgacgg
    25921 ttaaacctgc ctgagaacgg ctaattttgc gggtcgcaca ggtgcgatac ggatctttaa
    25981 tgataatttc ggttagcagg cgatcgcatg ggcaggccag gtagtcctcg attgacagcg
    26041 tttcgccgtt gccaaaaacc agttcagcat ccagtgctag caggacggga agcagcactg
    26101 actcttcctg ccgggcggcg atttcaccac caatggtcga ctgattacga acatggcgtg
    26161 agtaaacaaa accgagggct tcacgcagtg ctgcaggaat aaagcgcgca tcacgcagtg
    26221 gctgcaagcg agacattgca ccaatccgca gtgcaccgtt atcccagtca acccagtcca
    26281 gttccagatc ctgtaaggaa atggcaatct ttttatcggt acgggttggt gtagcattga
    26341 gtttgctgcc cccggcgaac cagacggctt catcctggta gcggcgcttc agttccagcg
    26401 cctgttcgac agagtcgggc ctgaaaaatt gttcaatcat cttctttcct tatttaaccc
    26461 tcttccctgc gggaagaggg catctgtcat ttatgccagc gcatccatcc gacgccacat
    26521 actggcagcg gcttttctgg cttgtgcata aatggaatcg caatcgaagt taaactgacg
    26581 gtcttcatag accatcacac cattgaccat cacgctgtga acgctgcctg agcccatacc
    26641 gaaagcgata tgcccggcga tattgtctgc cagcagcggc gtcggcgagt tgtaatcgca
    26701 aatggtcaaa tcagctttgt aaccggcttc cagaagccca aatttcgcgc caaagttgcg
    26761 gctcatcagt tcgttaccgt tagtcagggc tttggcaaaa ctgtcaggcc acagcggacc
    26821 acccgcatcg cgatgtttaa agaaggcaaa tttcatctct tcaaacatgt ccgaaccaat
    26881 gccgtccgtt cccaatgcca ggttgcggat gtcgctaagg tgatggttgt agccgacatg
    26941 gttgttcatg tttgaacggg cgttatgcac caggaacgca tcgcgctgat tgagtagggc
    27001 gatgtcatct ttcgacaagt acagcccatg agcgaccagc gttttgctgt cgatgaggtc
    27061 gaattgcgcc agtcgtgcca gcaggtcttt gccgtaccag tggtgactgt aggaaacgtc
    27121 gtaaaggtct tccgcagcgt gaatatgcaa accacggcct gtggctttca cggcttcacg
    27181 cagcatctcc agaccggcat ccggcacggt aaacggcgcg tgagcaccga tatgtgcttc
    27241 caccagatac ggctcgcttg tcgctttctt cgcctcatca atcaaacggg cgaaacggat
    27301 gttttcttct acaccttcct gcaactcttt gatgccgttg ttacggtcag tagtttcaaa
    27361 acaggtcatc gcgcgcaggc caacttttaa aaatgcgtcg cgcaatgtgg agagcgaccc
    27421 gccgatatac gccggcgagg catggtgatc gataaccgat gtacatccgc tcttaatcgc
    27481 ttccagggaa caaatcagtc cgctgtaata gagcgactct tcatcaaggg cgcgatcgag
    27541 ccgccaccag agatttttca gcgttgagat gaaatccggg caaggggcga tgtttgccat
    27601 aattccgcgg gaaagctccg agtaaaaatg gttgtgcgag cagacaattc ccggcatcac
    27661 aatccgacca tgcatctctt tgtagctggc atcggggtag cgttgcgtca gggcatcgcc
    27721 gatagcgaca atcacgtcat tttcgatagc gatatcaacg ccttcctgca ctttcgccgg
    27781 gtgtaactgc actgcggtga cattcttcag aatcaacatg attacacctc cacgcggccc
    27841 agcagataat gatgatgctg gtggacatgg ctgatgatgc ggcacatatc gttcagctcc
    27901 ggtggtacgt tgttaaactg accttcgctg tcgatgttta acacccagct ttggttattc
    27961 agacgtactc gtacccggca atcttccaca aggaagcctg gggtgctgct gttatcaaag
    28021 tcttgcgcca ggctgaagac ggtgattttg tcttcgtatg gtttaccgtt ccacggacag
    28081 aactgagcgc agttgccgca ttcgttacag taagcgtcga ggtgcagcgt ctggaatcta
    28141 ttctggaagc cagggactgc aatggagacg ttggcgcggt tcgggcagac atccacacac
    28201 ttgttgcaaa cgtagttaca ttcgaggcag cgagcggctt cctgggcgac aaacgcgtca
    28261 cgatcgtcac tgttcaccag cgtgatagag atatcgcctt tacgttgata gatttccgct
    28321 ggattgacgt tgttccagta tttatcgttc tggtgggaac ggatattttc ccgactaagg
    28381 atggcatcgg tcgcccgacg cgcggttccg acagcagcga caatggagga tggtccgcgc
    28441 tgtacgtcgc cgatcataaa gacgtcagtc agacgagttt cgccattatg gtcgacgtct
    28501 ggccagccgt ttttgtccag cggcacgccc atcgcattca gggcttcagt atcctgctgt
    28561 tcaccaatgg cggtgatcag gctgtctaca tgcagtgtta ctgtttcgtt ggtttcaacc
    28621 ggacggcgac gacctttctc atccggttcg ccaagcgaca taacgcgcaa ggttaaggtg
    28681 ccatcagcat cgaaacgttc cggattattc aggaaacgga actctacgcc gtcgtgcaac
    28741 gcttcttcat actcttcgcg ccatgcgggc atctcttgta gtgaacgacg gtaaacgatc
    28801 gttgcttttt ctacgcctgg aacgcgtaac gccgcacgag cgcagtccat tgcggtgtta
    28861 cccgccccga caacgaccac atgtttgccc agcttgagcg ctgtaccctt gttgtattca
    28921 cgcaggaagg ggagtgattt ccagacattt tggttgtcgc ccgccagttt cacaccgcta
    28981 tttttatcag tgccggtggc aatcagaaca tagtggaagc cctgattttt taactgttca
    29041 acggttaaat cgggtgagca gccatactca aatttcacgc cgtgagcggc aacaaaatcg
    29101 atatcgtgct gaattaactc tgcaggaata cggaactgag gaatgatatt tttcaccacg
    29161 ccgccagcat tggcttcgcg ttcaaacagc gtaaccggat ggcccgctct ggcaaggaag
    29221 taacctgctg ccagacccgc cggacctgca ccaatcacgg caaccggatg gcgtgaacca
    29281 gaaccggcgg gtttgtgcca gcgttgctta tattcatccc aacctttttc cagcgcgact
    29341 tttttcagtt cgcggatatt cagcgcactg tcgtaatcca ggcgggtaca gttgtattgg
    29401 cactggtgat cgcaaatatg accggtaatg gcaggtaggg cgttgcgttg gtagatgagt
    29461 tccagcgcgt cggcatagcg gtgttcgcca agcagacgga tgtattccgg aatatcttgc
    29521 ttgatagcgc aggcagtaac acagggggca acgtagcaat cggtcagcgg caggtcttct
    29581 gccacttcaa tacgctcttc tggcttccag tgtttctggg tgtattccat ggttaacgcg
    29641 tctgctgcca gtctgttcag tcgttcgacg tcaacatggt caagtcccca ggcgtcggag
    29701 ccttccagct cgcgcatgca ggcacttaag cgcagatagc cgccaggttt cagcaggtcg
    29761 gttgccatag taatagggcg aatacctgta tcaaaaatat cgcggatagt cagctgactg
    29821 gcaccaccgg aataagaaat gggcagtttg ccgtcaaagg cgcgagagag aactgctgca
    29881 acattgatgg agagcgggaa cagcgcacgg cctgacatat acatctcttc accaggcagt
    29941 gcgcctttat tgttgatggt gccgagagtg ttagtcagtt ttacgccaaa gccgagtgat
    30001 ttttcttttg ccagtaccat caggcgctcc agcatttcca gtgcttgcgt cagcttgagg
    30061 tcgtgatcaa atgactcttc ttttaagcct atgtaaccga aaccgcagac atcgaggatc
    30121 tcacgaacac gcgcgtaccc cagtaaggtc gggttaagtt tgacaaaggt gttgagccct
    30181 ttttcttcca gcatgtagcg gcaaatggct tcgatttcat gcggaggaca gccgtgcatg
    30241 gtggagaggg tgacgccctg caccatactg gtggggatgc gagcgggtaa ggcttgcaag
    30301 ttttcgcgtt tttcctgcaa tccgtggcgg gctaaaaatg cgtcatcctg gagtaattta
    30361 ttcagcgtat cgcgatattg agcgaatttc ggatggtcag atgcgtccat catattgtcg
    30421 atgaactgtt gcatcggcgg ttgcttaata ccttcgaggt tgtaaccgac gctcatatta
    30481 aagatgaacg atttaccgga atcagaaggc tgtaacatcg cttcgagaag gtgcagggca
    30541 aaccaggctt tgaggtattc atcccaggct ttaagcaagg taaattcggt agaccattcg
    30601 gtgttaaagc attcgtcttc ggcatcaata cagggctttt ccagctccag gcggtcaaga
    30661 atttggacgg tttttagttc gatgaagcgt ccgccagtca gccaggaagt gacaatattt
    30721 tgcgcgagct gcgtgtgcgg gccagcggca gggccgacgg gagtggcaca ggtttcaccg
    30781 aagacgctaa cagttttacc ttttacaggt gagtaaaact gttgctcggg aataccaaag
    30841 attgagcgtt gttgttggta ttcatcaaat atgcgcgtca aaagttcctc aaacggaatg
    30901 ggacgcataa tatcccccat aaccctctcc ttagcttata cagatatcat tgagtgaccg
    30961 gtgttgagaa atccggtggt ttagggggat agagcaacaa tcgcaccact cttgccaggt
    31021 ttggcgggaa atataaaaaa atgtgaggtg gctcgaaact taaaggcttt ttgtgaatga
    31081 actcacaggt gccaaatgag aattaaaatc actcctgaaa attattattg tcagcccatt
    31141 tatcactttt tctcttttct caaaatcagt gattccttat cattatgata aagagtgctg
    31201 gagagattaa atatttgaac gcaatcgttt aactacattt tctaaatttt agtatacctt
    31261 ttttgcgctg taataaagtc gtccggagta tcaatatcta aaattatttc tgcattttct
    31321 atttcgacgg aataatgatc tcccataagg agtgcctgac gcatattggt gacattgggt
    31381 cgctggattg cctgcatcag gcatggtttt gacactaaaa tcggatggcc ggggatgcca
    31441 ttatggagtg gcagtattgc gccatcattt cgtaacgacc agatttttct aaaaatatcg
    31501 atggtgaggg ttggcatgtc accgtgggtg agaaaacaat gttctgtttg cactgcgggt
    31561 actgcggcct tgacggatgt tagtaaaccc tgcgcataat ctgggttgtg aataatagtg
    31621 atattgctct ggttcgcata acgttcgtgc agttcattac cacgatagcc ggtgactaaa
    31681 ataattcggc tacaaaactg caacgcattt ttgatacttg tatcaagaat tgttccctgt
    31741 tcccagggga acatcatttt ccattgcccc attcttgatg ataatccggc agcggtaatt
    31801 atacagtcga tggctgacat agtggttcca ttatttatag gggctaataa cgcctgataa
    31861 ttgcgttact tcctgatgga ttaagttggg gataatttat cccagagagg tcataaagac
    31921 tcgtgaaaag tatagttgac ccatcggcat tagtcattga tttaggtgcg cagaaacgtc
    31981 ccactgtgat ttctgttgtt ggtgctgggg ggaaaaccag cttgcttttt tggctggcag
    32041 aactgctcca ggctagcggc aggcgtgtgt taatcacgac gacgacacat atgtttatgc
    32101 caacgtctca ctggcccgtg gttttctgtc gtgatcccgc catgcttcct catgcgtctc
    32161 ttacatcccc catttcattt tgttttcaca gctggaaagc gaaccaggga aaagtgcagg
    32221 gatttacgcc agaagcgatt gatgcactgg tgcaacgacc agagtgtgac gtaattctca
    32281 ttgaagcaga tggctcgcgt ggaatgccgt taaaagcgcc tgatgagcac gaaccttgca
    32341 tacccaaaag cagttgctgc gtgattgctg tgatgggagg acatactttg ggcgcgaaag
    32401 tgagcacaga aaatgtccat cgctggtcgc agtttgctga tattaccggg ttaacacctg
    32461 atgcaacctt gcaactgagc gatctcgttg cgctggttcg ccaccctcag ggggcgttta
    32521 aaaacgtacc gcaaggttgt cggcgagtct ggttcattaa ccgtttttct caatgtgaga
    32581 atgcgattgc acaaagcgag ctccttcaac cgctgcaaca acacgacgta gaggcaatct
    32641 ggctgggcga tatacaagaa catcctgcaa tcgcgcgcag atttgtgaat tagcggcccg
    32701 ggtattccgg gttactgaaa ggatatccat tgaggttggt atgaatattt tcacagaggc
    32761 tgcaaaactc gaagagcaaa attgtccgtt tgcgatggcg caaattgttg atagccgagg
    32821 ctcgactccc cgccattctg cacaaatgtt agtgcgcgcc gatggttcta tcgtcggtac
    32881 aattggtggc ggaatggttg agtggaaggt gattgaagag tcacttcagg cattgcagga
    32941 acgtaagccg cgattattcc atggacgtat ggctcgtaac ggtgcggatg ctgtcgggtc
    33001 agattgtgga ggtgcaatgt cagtatttat cagcgtccat ggtatgcgtc cacgtctggt
    33061 gttgattggc gcggggcatg tcaaccgggc gatagcccag agtgcggcgc tattaggatt
    33121 tgatattgcc gttgccgata tttatcgcga aagcctcaat cctgaactat tcccaccatc
    33181 aaccacgctt ctccatgctg agtcgtttgg tgcggcagtg gaagaactgg atattcgccc
    33241 tgataatttt gtcctgattg ccacgaataa tcaggatcgt gaagccctcg acaaactcat
    33301 tgaacagccc attgcatggt tggggttgct ggcaagtcgc cgcaaggttc agcttttcct
    33361 gcgtcaattg cgtgagaaag gcgtggctga agaacatatt gcccgtttac atgcgcccgt
    33421 tggttacaac ataggtgcgg aaacgccgca ggagatcgcc atcagcgtgc tggcagaaat
    33481 attacgggtg aaaaataacg cgccgggtgg gctgatgatg aaaccttctc atccttccgg
    33541 acaccagctg gtggtgattc gcggtgcggg agatatcgcc agtggtgtgg cgctacgtct
    33601 gtatcatgcg ggttttaaag tgatcatgct ggaagtggaa aaaccgacag tgattcgttg
    33661 taccgtggcg tttgcccagg ccgtgttcga tggcgaaatg acggtcgaag gcgtcactgc
    33721 tcgcctggca accagctctg cggaagcgat gaaacttacc gaacgcggat tcatccctgt
    33781 gatggtagat cccgcctgtt cattgcttga tgaactgaaa ccgctttgcg tggtggacgc
    33841 tattctggcg aaacagaatt tgggaacgcg ggcagatatg gcaccagtaa caatcgcgct
    33901 tgggccgggc tttactgcag ggaaggattg tcatgcggta attgaaacaa atcgcgggca
    33961 ctggctcggt caggtgattt actctggttg tgcgcaggag aataccgatg ttcctggcaa
    34021 tattatgggg cataccaccc gacgggtgat ccgtgctcct gctgcaggca ttatgcgatc
    34081 caacgtgaaa ttaggcgatc tggtgaaaga gggcgatgtg attgcctgga ttggtgagca
    34141 tgaaattaaa gcaccgttga cggggatggt gcgtggcttg ttgaacgacg gcctggcagt
    34201 ggttggtggt tttaaaattg gtgatatcga tcctcgtggt gaaaccgctg atttcaccag
    34261 cgtttctgat aaagcccggg cgattggcgg cggcgtactt gaggcgttaa tgatgttgat
    34321 gcatcagggc gtgaaagcga caaaagaagt gctggaagtg gcttaaatga aaatggccgg
    34381 atggtattac ccacccggcc ttaaaattac tgacaaatca ccgttccggt tttgccttca
    34441 atcccttctt tcgctttgct caatacggta atcagtgctt cacgacctgc gcgagagcga
    34501 gcaaacgagg cggcagcttc cactttaggc agcatagaac ctttcgcaaa atgaccttct
    34561 tcgataaagc gttcagcatc acttaatgac aggcgatcga gccactgttc attctcttta
    34621 ccgaagttaa tggccacttt ttctaccgca gtcagaatga tcagcatatc ggcatcaatc
    34681 atttctgcta aacgggcgct ggcccagtct ttatcgataa ccgcgctggc accacgcaga
    34741 tggttacctt cacgaataac agggatacca ccaccgccaa cggcgatcac cacctggcct
    34801 gcatctacca gagctttaac cgtttctttt tcaatgatat caaccggttt tggcgaggca
    34861 acaacacggc gatagccgcg accagcatct tctttcagag tgtaaccctg ttttgtcagt
    34921 tgctctgctt cttgctcggt aaagaacgaa ccgatcggct tggtcgggtt gaggaatgcc
    34981 ggatcgttag cgtcgacttc gacctgagta accagcgttg ctacaggttt attaatgccc
    35041 cgagaaagca gttcttctct taacgcgttt tgtagatcgt aaccaatata accctggctt
    35101 aaggcaacac agacagacat cggcagcatt ggcgagtgtg cttcagtttt tgcggcagct
    35161 tcaaaggctt ggttaatcat gcccacctgc ggcccgttac catgagtgac gataacttca
    35221 tgtccttggg caattaaatc aacaattgcc tgagaagtaa tttttaccgc tttcatttgt
    35281 ccggccaggt catcgcccag cgcattcccg cccagggcga gaacaatttt cttactcatt
    35341 tttcctcact catcattctt gattgtgtat agatccctga cagtggttag agcacgggag
    35401 ggacaaacgg tttgcgtcgc aggaaacgac ctcgcccggc tttgcctgta aaggtgccgt
    35461 cacagaaaat cgtttcacca cgggataagg ttctgacaat cgcgccctga caggtaaaac
    35521 cctcccatgg cgagtagtcg gcgttgtcgt ggagatggcg atgctgaatt tgttggctct
    35581 gacgtgggtc gataatcacc acgtcgccat cggaaccggg cgctaataat cctttttgcg
    35641 gccacaggcc aaacagcctg gcgggcattg cgctggttaa ttcaacaaag cgttccggtg
    35701 ttatacgtcc cgtcatcacg ccactggaaa acagtaactg catgcggttt tccacaccgg
    35761 gtaagccatt tgggcagcga ctgaaatcgc ctttagaaat ttgcaggcgt tgagccatcg
    35821 agaaggtgca gtgatcggtc gccaccacgt caatcgcacc atcgctgatg ccacaccaca
    35881 gtttgtcctg ctcgcgtacg ttacgcagcg gtgggctaag aatgaacttc atgccatctt
    35941 ctgtatcgta actgcgttcg tccaacagga gatattgtgg gcaggtttca acccagactg
    36001 gctggtgatt cgcacgggca agacgcagat aatccagacc taagccgtta gacaggtgca
    36061 cgatatagag cggggcgtta ccggcaattt gtgccaggtt aatcatgcgg gcgatggctt
    36121 ccgcttcgca ttccagaggg cgactcaagg catgatagcg cggcgcggtt aaccctgcgg
    36181 cgataaattc cgcccgcttg ctggcgatag ccgcatcatt ttccgggtgc acggtggtca
    36241 gcgcgccgga ttcatgcaag cggcgtaatg cctgcaaaac ctcgtcatcg ttgagtttgt
    36301 attgataggt taaatagagt ttaaaactgc tcagtccttc ctcgaccatc atcgggattt
    36361 cgtcgaggat tgcgtgatta atgtgctgga tcacaccgtg aaagctgtaa tcgatgaccg
    36421 ctttatgggc ggcataacca cgataaacct ccagttgatg gcgtaaccga cagccgtttg
    36481 ggccaaatcc catatggtca ataatggttg ttgtaccgcc acacgcagct gcgcgggtac
    36541 cggtaaaaaa atcatcacaa ctgcgcgcga tgccgacatc aatattgaaa tgcgtatgga
    36601 catccacgcc gccagggaaa acgtaacagc cagtggcatc aatttcttca tacgggagct
    36661 gcggcgaaat attgttgccc aactggcgaa caatcccgct ttcaatcagc aaatcctgtt
    36721 tggcttgtcc atctgcgtta acgacagtgc cgtttttgat caatacgcgc atagcaaact
    36781 ccaaaaaccc tccggcgaac cggaggggaa taagtgaaga gtaattactc tgttgccagc
    36841 cagcttaacg ggattgcagc gtacatcgcg gcacaggtca ccaggtgaga tttccaggtt
    36901 ttttcgttag gtgcatgcgc ttcaggttct ttacccgggc caaagccgat caccggaatg
    36961 ccgtgacggc ccatgataga tacgccgtta gttgagaagg tccacttatc aacaaccggc
    37021 gctttgccaa acagcccttc gtaggcattc accagtgctt taacggtgaa gtgatcttct
    37081 tccactttcc aggtcgggaa gtagcattcg gttgggtaaa ccaggccagt ccaggacgga
    37141 cggtcgtagt tgtacataga aacaaccgcg ttagctttct gtactgcagg cagggcgcgg
    37201 atttcgtcca gcgcgccttc ccaggtttcg ccccaggtca gacggcggtc aatagagacg
    37261 gcgcagctat ctgctacagc gcaacggctt ggggatgtga agaagatttc agaaacggtg
    37321 agggtgcctt tgccgaggaa ttcgtcataa cccagacgtt gggagagttc ttgtaattcg
    37381 ccaagaatcg gacccatttt gaaaatggcg ttgtcaccgc gttctggcgc agaaccgtgg
    37441 cagctaacac cctgaacatc aatacgaatt tccatacgac cgcgctgacc acggtatatc
    37501 tggcagtcgg ttggttcggt actgaccaca aattccgggc gaatgccgga ttgttcaata
    37561 atgtactgcc agcacagacc gtcgcagtct tcttcctgca cagtaccggt aaccagcagg
    37621 gtatattcat cttccagacc gaggtcttta atgattttac cggcataaac catagatgcc
    37681 atgccgcctt cctggtcgga agtaccgcga ccaccgatta gctcatcagt ttccatgcct
    37741 tcgtacggat cgaagtccca gtttttgatg ttgccaatgc cgacggtatc gatatgagcg
    37801 tccattgcca ccagacgcgg gccgtggccg atataaccga gaacgttgcc catcgggtcg
    37861 atttcaactt tatcgaagcc gactttttcc atctcttctt taatacgatg tactacgcgt
    37921 ttttcgtcgc agctttcact gggaatagca accatgtcgc gcaggaagcg agtcatgtcc
    37981 gcctggtaat cttttgcttt ttcaagaatc agtttgaatg gaatattctt agccattatt
    38041 tttctccaga ttttttactc cccgttttgc agggagtagt tccaataggg tgattaaggt
    38101 gctacagcgt gtttgccttc ccagacaact tcgcggtagt gcttcacgtc ggtgtcgcct
    38161 tcagtgctga taaccagcac tacggcatct ttgtccagcg ccagtttttc catcaggctt
    38221 tgatgttgcg ggtgataatg aaccgctgcg agaacgccca aaccgacagc gccggattca
    38281 ccggagatga tgcgcgggtc gttgccgtac ggattaccca gcacgcgcat acctaatgcg
    38341 gcaacgctgt cctggcagga gatgaattgg gtggcgcagt tacgtaggat ttcccagccc
    38401 agcgggttag gttcgccaca ggccaggccc gccatgatgg tggccatatc accgccaacg
    38461 ttgacgatgt cgcctttgac gccggagcga taaatacagt cagctttgtc aggttcaaca
    38521 ataatgctgt gcagattttg cgggctgtag acgtcgacca gataacccag cacaccaccg
    38581 gccattgctc cgacaccggc ttgcagcaga acgtgcgtcg gggttacgcc catttcacgc
    38641 atttgctcga cggcttcatc tgccagggtt gcgtagcctt gcatgatcca ggttgggatt
    38701 ttggtgtaac cttcccatgc cgtgtcctgt accacttccc agccgtgctg ctgcgcgtgt
    38761 tgcatggtca ggcgaacggt atcgtcatag ttcatatccg tgacgatgca ctcggcaccg
    38821 aggttcagaa tggcgtcaac gcgttcctga gcagaacctt tcggcatgta aatcaccgca
    38881 ttctgtccga gttgctgtgc tgcccacgcc acaccgcgcc cgtggttgcc gtcggtggtg
    38941 gtcgcgaaag tcattttttc gccgatggca tttttcaggt gctcaaatga cagcgtttcg
    39001 atatcaagat gatatttttc acacaataat tgagcgatgg cgtacgcacc gccaagcatt
    39061 ttgaaggcgt tcagaccgaa tcgttttgat tcgtctttga cgagaatttt cttcacacca
    39121 aaaaggtttg cgagatcgtc cagcgcacaa agcggtgttg ggcgataacc agctattttc
    39181 tggtggaact ggcgcgccag tttggcctgg ctttgcgaaa agagcggtga tgtttcgccg
    39241 ttgaaaaatt tgttatcggc gatatcaatc ttcaatgaga aaacggacat atcctatcct
    39301 tttggcctct ggaaatagag ggccgaccag cagatcggcc cgaggggtta tttcacgcgt
    39361 tcttgcgctt ctttcagcag ttgctcgagc agtgcacctg gtttggcgta tttacgggac
    39421 aggatcatcg cggcgatgat ataaggcttc cagctggctt ctttgtaggt agcgatacgg
    39481 tatttttcga atacgccttc agtcacttca ccttctttgc aggatacgcc gctgatatca
    39541 gctggcaggc agtgcatgta cagggcttcg ccatcacggg tcagttccat catctcttca
    39601 gtacaatgcc agtctttgtg ttgcgcgttc tgtgccagac actgtttttc cagtgctttt
    39661 aagccttcgt ggtcgttcgc acgcagcaat tcagtacgct cttccatcac tttgtaaggt
    39721 gcccatgact tcggataaac gatgtctgcg tctttgaagg cttcttccat gctggtgacc
    39781 tgacggaagc taccaccgga ggctttagca ttgtttttcg ccacttcaac cacatccggg
    39841 atcaggtcgt agccttccgg atgggccaag gtgacatcca taccgaagcg agtcatcaga
    39901 ccgatgatgc cttgtggtac agagagcggt ttgccatagc ttggagagta ggcccaggtc
    39961 atggcgattt ttttaccttt caggttttcc agtgaaccaa agtgttcacg taaccacgcg
    40021 aggtcagcca ttgactgagt cgggtggtca atatcgcatt gcaggttcac taaagccgga
    40081 cgctgtggca gtacaccctg cttgtaaccg tcatcaagtg cagcgccaac ttcacgcata
    40141 taggcgttgc ctgcgcccag atacatatcg tcgcgaatac caatagcgtc ggcgcagaag
    40201 gagatcatat tggcggtttc acgcacggtt tcgccgtgag cgatttgtga tttgccttcg
    40261 tcgagatctt gttgtgcaag gccgagcagg ttaagcgcgg aagcataaga gaagcgggta
    40321 cgggtggagt tgtcgcggaa tacggaaata cctaatccac tattaaagac tttggttgag
    40381 atgttttcag cacgcagtgc ttttaatgct gcggcaacgt ccagtacttg tttcagttca
    40441 tctggcgtct gttcccacgt taacaaaaaa tctttctcgt gaaggtgaga ggtcagcgaa
    40501 ttgatatcct taatcagctc attaacagtt ttcatcatac agtcccggta gtggaatgac
    40561 aggcaaagtg gtttatttca ctgtgacttg ccaaagtgat tggtgttttc atagctatac
    40621 ctgggactaa caataagtgt gccagaatta tccgcgcggc tgcaaaagac taatttgacg
    40681 gataatgtgt ggggttgttc acaaattgat gccagcttct ggttgtttgt aaaatacgaa
    40741 aacgtttggc tttttttatc gcgttattta tttgctgcta agtatcagaa ataagataaa
    40801 ggggcaggct atctctcttc agtttgataa actcaccgcc gtaattctca tattgatata
    40861 gaaatgtgat atctcttcca taattgcatt agaaaatgat aaaaatctta tgtttttatc
    40921 aaagccaatt tcgcctgagt taatatcatc atgatctgtg aaccatcaac gtcttcaatg
    40981 atcgactttt aagtaagtct ggaaggtaaa gctatggagc ttgctactac gcagtcagta
    41041 ttgatgcaaa ttcaaccgac aattcagcgt tttgccagaa tgcttgccag cgttttgcag
    41101 tttgaggttg agatcgttga tgaaaacttg tgtcgcgtcg ccggaacggg cgcgtatggg
    41161 aagtttcttg gtcgccagtt gagcggcaac tcacgcctgc tccgccacgt cctggaaacg
    41221 aaaactgaaa aagttgtgac acagtctcgc ttcgatcccc tttgcgaagg ttgcgatagt
    41281 aaagaaaatt gccgcgaaaa agcatttctg ggtacgcctg tcattttaca ggatcgttgt
    41341 gttggggtga taagtttgat tgccgttacc cacgagcaac aagagcatat cagtgataat
    41401 ttacgcgaat tttctgatta tgttcgccat atatccacca tttttgtttc gaaacttctg
    41461 gaggatcagg ggccaggaga taacatcagt aaaatattcg cgaccatgat cgataatatg
    41521 gatcagggcg tattagttgt tgatgatgaa agtcgggttc agtttgttaa tcagactgcc
    41581 ttaaaaacac ttggtgttgt acaaaataat attattggga aacctatccg tttcagacca
    41641 ttaacatttg agagtaattt tactcatgga catatgcagc atattgtttc gtgggacgat
    41701 aaaagtgaat taatcattgg tcaattgcat aacattcagg gccgacaatt atttttaatg
    41761 gcatttcacc aatcgcatac cagtttttct gtagcaaatg cacctgatga accacatatt
    41821 gaacaattgg ttggcgagtg ccgtgttatg cggcaattaa aacgactcat tagccgtatt
    41881 gcacccagcc catccagcgt tatggtggtt ggtgaaagcg gcacgggtaa agaagtcgtc
    41941 gcccgagcaa tccataagtt gagcggaaga cggaataaac cctttattgc tatcaactgt
    42001 gccgcgattc cggagcagct tctggaaagc gaactgttcg gttatgttaa aggcgcattt
    42061 actagtgctt ctgccaacgg taaaacaggg ttgattcagg cggcgaatac gggcacgctg
    42121 tttctcgatg aaataggtga tatgccatta atgttgcagg ctaaattact gcgcgctatt
    42181 gaggcgcgtg aaattctgcc gattggtgcc agtagcccaa tacaagtcga cattcgcatc
    42241 atttctgcaa ctaatcagaa tttggcccag ttcattgccg aaggtaaatt ccgcgaagat
    42301 ctcttctacc gacttaatgt tatcccgata actctgccac cgctgcgtga acgtcaggaa
    42361 gatattgaac tattggtgca ttacttttta catctgcata cccgtcgtct gggatcggtt
    42421 tatcctggca ttgctcccga tgtcgtcgaa atattgcgta agcatcgttg gcccggaaac
    42481 ctgcgcgagt taagcaattt gatggaatat ctggttaacg tggttccttc aggtgaagtt
    42541 atcgacagca cgctattgcc gccaaatctg ctgaataatg gcacaacgga gcaaagtgat
    42601 gtaacagagg tcagtgaggc gcacctgtca ctcgatgatg cgggcggcac ggcgctggag
    42661 gagatggaaa agcaaatgat ccgcgaggcg ctttcacgtc ataacagcaa gaagcaagtt
    42721 gctgatgaac tgggcatcgg cattgctacg ctctatcgca agattaagaa atatgagttg
    42781 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
    42841 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
    42901 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
    42961 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgcgccgga
    43021 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
    43081 gcggatctcc tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
    43141 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
    43201 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
    43261 aggcatcccg ttaatggtgc attcgatggt aattgtttcg ctgtgattca ttgcaatttt
    43321 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
    43381 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
    43441 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
    43501 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
    43561 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
    43621 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
    43681 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
    43741 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
    43801 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
    43861 gcgacgttac ggatctgcgg tccagcaatg gacgtggccg cagcacataa cgccgggaga
    43921 tgacgttgag ttataggagc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
    43981 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
    44041 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccatcggcg
    44101 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
    44161 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
    44221 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
    44281 cttcaccgcg ttacgaatag cagcggcaac aggaattatt ggtggctcac ccagtgactt
    44341 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
    44401 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
    44461 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
    44521 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
    44581 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
    44641 aaacgccgat gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
    44701 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
    44761 tcgttcaacc agcacgatat ggccttttat cagggtcaga ttcatcgctg actgatgtag
    44821 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
    44881 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggagcgaacg gcgtaacgtc
    44941 ggtatcttga gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
    45001 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
    45061 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
    45121 ggtgttagag gtatagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
    45181 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
    45241 ctccggcaac cctgcgctgt aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
    45301 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
    45361 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
    45421 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
    45481 gtaagcgact ttattcctcc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
    45541 ggtgttagac agaacatcca gactataacc tttcaatgtt ccgtcgcggt tcacgcccat
    45601 ttgcccgtca atggtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
    45661 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgctg ccattggctc
    45721 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
    45781 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
    45841 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
    45901 ttccatgtga caatgttgaa taacgggggt ctgatagtgc ccctgtacct ggtagtcggc
    45961 ggcatcgatt gtttgttgga cattacccgt cgacatcgtg ctttgtttca gtaaattgcc
    46021 accgttatgg attggtgcag cgtcttctgc cagcgccgct tctggcgtgg tgataacggg
    46081 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
    46141 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
    46201 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
    46261 aatatcaggc acatcttccc aggtaaaaat cgccagtacg cccggcaaac ttctggcttg
    46321 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcata
    46381 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
    46441 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
    46501 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
    46561 cgccagaaac gacgataagc gttaggggag gtaggaagtg tgatgacgtt aaaaaaacac
    46621 agatatgata cgaaacagga ctctgaatag gggcagtgat acgtatcaat atgagtggcg
    46681 ataaaatcaa tgtgataaat tgggattatt acgcatcagg atgggaaata gggtaaaaag
    46741 tcaggaagtt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
    46801 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
    46861 ggctggctgc tatgataagg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
    46921 cgcctgaata aaaaatctct gggtatcgtg atgttgttat cggttggact gcttttggcg
    46981 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
    47041 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagaactg
    47101 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
    47161 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gaccaaaacc
    47221 gcatcggtta caccgtcatc agcggtaccg aaatcatcct ggccgccagt agggcaacgt
    47281 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
    47341 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
    47401 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
    47461 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
    47521 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
    47581 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
    47641 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
    47701 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
    47761 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccg cttttgctgt
    47821 atctgatttt ctatattatc ttaattcaat cgcttcagac ttttcgcaat cgccgtaata
    47881 tttccccatt ttgttgtaaa taaaaagcgt ttacgcaatg caatcctttt gtaacaaccc
    47941 aggtaaaaca ccttattttc aagttgttaa ataagtggtt atttacgctt ttaacaaacg
    48001 attgatggaa tattttatga aagggaaatc tgcattaact cttctgctcg ctggtatatt
    48061 ttcgtgcggc acatgccagg cgacaggggc agaagtgacg agtgaatctg tatttaacat
    48121 tcttaattcc acgggggcag caacagataa aagttattta tcgttaaatc cagataagta
    48181 ccctaattat cgtcttctga ttcactcagc caaattaaaa aatgaaataa agagccacta
    48241 taccaaagac gaaattcaag ggttgttaac attaacagaa aacacacgaa agttaacgtt
    48301 gacggagaaa ccctggggaa catttattct tgcttctact ttcgaagatg acaaaacagc
    48361 ggcagaaacc cactatgatg ctgtctggct gagggatagt ttatggggct atatggcatt
    48421 agtatcagat cagggaaata gtgtagccgc aaaaaaggtt ctgctcaccc tgtgggatta
    48481 tatgtctacg ctcgaccaga ttaagcggat gcaggacgtc attagcaacc ccaaacgact
    48541 ggatggagtt cccgggcaaa tgaatgctgt gcatatccga tttgacagca actcccccgt
    48601 gatggcagat gtgcaggaag aaggcaagcc gcagctgtgg aaccataaac aaaatgatgc
    48661 gttaggtctt tatcttgatc tccttataca agcaatcgac actggtacaa ttaacgctga
    48721 agattggcaa aaaggtgacc gattaaaatc ggttgcgctg ctgattgctt atctggataa
    48781 agccaatttc tatgttatgg aagattccgg agcatgggaa gaagatgccc gattaaacac
    48841 atcttccgtt gcactggtga cctctggact ggaacgtttg tcgaacttac tctcgaagaa
    48901 agactctgtt tttgtctctg atttactacg cgaagctaaa gccaatgaac tggatgaacc
    48961 tctttcaaca acgaggttga accacctcat agataaaggt tatgaaagaa taacgttaca
    49021 gcttgattta gggggagaat ccccaggtta tctggaaaaa gataaacatt acagagaagc
    49081 cgatgcggct ttacttaatg ttatttatcc tgcaaatctt gcaaagataa acaccagaag
    49141 aaaagagcag gttctaaaaa tagttaaaaa actagcaggt ccgtatggta taaaaagata
    49201 cgagaaggat aattatcagt cggcaaattt ttggttcaat gatattaaaa ccgataccga
    49261 tcaaaattca catgcgaaaa g
//
LOCUS       DAZPEP010000012        46769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZPEP010000012 DAZPEP010000000
VERSION     DAZPEP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.012"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHUT9_000713"
                     /gene_synonym="thiJ"
     CDS             complement(17..868)
                     /gene="pdxK"
                     /locus_tag="ACHUT9_000713"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFR1347269.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            973..1341
                     /locus_tag="ACHUT9_000714"
     CDS             973..1341
                     /locus_tag="ACHUT9_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFR1347270.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHUT9_000715"
     CDS             complement(1344..2255)
                     /gene="cysM"
                     /locus_tag="ACHUT9_000715"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFR1347271.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHUT9_000716"
     CDS             complement(2389..3486)
                     /gene="cysA"
                     /locus_tag="ACHUT9_000716"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFR1347272.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHUT9_000717"
     CDS             complement(3476..4351)
                     /gene="cysW"
                     /locus_tag="ACHUT9_000717"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFR1347273.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHUT9_000718"
     CDS             complement(4351..5184)
                     /gene="cysT"
                     /locus_tag="ACHUT9_000718"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFR1347274.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHUT9_000719"
     CDS             complement(5184..6200)
                     /gene="cysP"
                     /locus_tag="ACHUT9_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFR1347275.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHUT9_000720"
     CDS             complement(6371..7162)
                     /gene="ucpA"
                     /locus_tag="ACHUT9_000720"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFR1347276.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHUT9_000721"
     CDS             complement(7291..8148)
                     /gene="murR"
                     /locus_tag="ACHUT9_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFR1347277.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            8312..9208
                     /gene="murQ"
                     /locus_tag="ACHUT9_000722"
     CDS             8312..9208
                     /gene="murQ"
                     /locus_tag="ACHUT9_000722"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFR1347278.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            9212..10636
                     /gene="murP"
                     /locus_tag="ACHUT9_000723"
                     /pseudo
     CDS             9212..10636
                     /gene="murP"
                     /locus_tag="ACHUT9_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHUT9_000724"
     CDS             10641..11945
                     /gene="pbp4b"
                     /locus_tag="ACHUT9_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFR1347279.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHUT9_000725"
     CDS             complement(12003..12902)
                     /gene="yfeX"
                     /locus_tag="ACHUT9_000725"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFR1347280.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(12998..13573)
                     /locus_tag="ACHUT9_000726"
     CDS             complement(12998..13573)
                     /locus_tag="ACHUT9_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFR1347281.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(13634..14083)
                     /locus_tag="ACHUT9_000727"
     CDS             complement(13634..14083)
                     /locus_tag="ACHUT9_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFR1347282.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(14070..14495)
                     /locus_tag="ACHUT9_000728"
     CDS             complement(14070..14495)
                     /locus_tag="ACHUT9_000728"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFR1347283.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            14709..15578
                     /gene="amiA"
                     /locus_tag="ACHUT9_000729"
     CDS             14709..15578
                     /gene="amiA"
                     /locus_tag="ACHUT9_000729"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFR1347284.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLPGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            15582..16481
                     /gene="hemF"
                     /locus_tag="ACHUT9_000730"
     CDS             15582..16481
                     /gene="hemF"
                     /locus_tag="ACHUT9_000730"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFR1347285.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHUT9_000731"
     CDS             complement(16487..17539)
                     /gene="eutR"
                     /locus_tag="ACHUT9_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFR1347286.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHUT9_000732"
     CDS             complement(17585..18085)
                     /gene="eutK"
                     /locus_tag="ACHUT9_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFR1347287.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHUT9_000733"
     CDS             complement(18098..18757)
                     /gene="eutL"
                     /locus_tag="ACHUT9_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFR1347288.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHUT9_000734"
     CDS             complement(18767..19654)
                     /gene="eutC"
                     /locus_tag="ACHUT9_000734"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFR1347289.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHUT9_000735"
     CDS             complement(19675..21036)
                     /gene="eutB"
                     /locus_tag="ACHUT9_000735"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFR1347290.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNRSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHUT9_000736"
     CDS             complement(21048..22451)
                     /gene="eutA"
                     /locus_tag="ACHUT9_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFR1347291.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHUT9_000737"
     CDS             complement(22448..23674)
                     /gene="eutH"
                     /locus_tag="ACHUT9_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFR1347292.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHUT9_000738"
     CDS             complement(23991..25178)
                     /gene="eutG"
                     /locus_tag="ACHUT9_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFR1347293.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHUT9_000739"
     CDS             complement(25168..26004)
                     /gene="eutJ"
                     /locus_tag="ACHUT9_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFR1347294.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(26015..27418)
                     /locus_tag="ACHUT9_000740"
     CDS             complement(26015..27418)
                     /locus_tag="ACHUT9_000740"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFR1347295.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHUT9_000741"
     CDS             complement(27430..27717)
                     /gene="eutN"
                     /locus_tag="ACHUT9_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFR1347296.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHUT9_000742"
     CDS             complement(27824..28117)
                     /gene="eutM"
                     /locus_tag="ACHUT9_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFR1347297.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHUT9_000743"
     CDS             complement(28156..29172)
                     /gene="pta"
                     /locus_tag="ACHUT9_000743"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFR1347298.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHUT9_000744"
     CDS             complement(29169..29972)
                     /gene="eutT"
                     /locus_tag="ACHUT9_000744"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFR1347299.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHUT9_000745"
     CDS             complement(29969..30670)
                     /gene="eutQ"
                     /locus_tag="ACHUT9_000745"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFR1347300.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHUT9_000746"
     CDS             complement(30645..31124)
                     /gene="eutP"
                     /locus_tag="ACHUT9_000746"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFR1347301.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHUT9_000747"
     CDS             complement(31137..31472)
                     /gene="eutS"
                     /locus_tag="ACHUT9_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFR1347302.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(31766..34045)
                     /gene="maeB"
                     /locus_tag="ACHUT9_000748"
     CDS             complement(31766..34045)
                     /gene="maeB"
                     /locus_tag="ACHUT9_000748"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFR1347303.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            34334..35284
                     /gene="tal"
                     /locus_tag="ACHUT9_000749"
     CDS             34334..35284
                     /gene="tal"
                     /locus_tag="ACHUT9_000749"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1347304.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            35304..37307
                     /gene="tkt"
                     /locus_tag="ACHUT9_000750"
     CDS             35304..37307
                     /gene="tkt"
                     /locus_tag="ACHUT9_000750"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1347305.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(37403..38446)
                     /locus_tag="ACHUT9_000751"
     CDS             complement(37403..38446)
                     /locus_tag="ACHUT9_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFR1347306.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(38572..39147)
                     /gene="nudK"
                     /locus_tag="ACHUT9_000752"
                     /pseudo
     CDS             complement(38572..39147)
                     /gene="nudK"
                     /locus_tag="ACHUT9_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
     gene            complement(39215..41194)
                     /gene="aegA"
                     /locus_tag="ACHUT9_000753"
     CDS             complement(39215..41194)
                     /gene="aegA"
                     /locus_tag="ACHUT9_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFR1347307.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            41400..43100
                     /gene="narQ"
                     /locus_tag="ACHUT9_000754"
     CDS             41400..43100
                     /gene="narQ"
                     /locus_tag="ACHUT9_000754"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFR1347308.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            43264..46377
                     /gene="acrD"
                     /locus_tag="ACHUT9_000755"
     CDS             43264..46377
                     /gene="acrD"
                     /locus_tag="ACHUT9_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFR1347309.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(46476..46535)
                     /gene="ypfM"
                     /locus_tag="ACHUT9_000756"
     CDS             complement(46476..46535)
                     /gene="ypfM"
                     /locus_tag="ACHUT9_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFR1347310.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        10705 a        13155 c        12118 g        10791 t
ORIGIN      
        1 ccatcggcgc cattttttat gcttccgcca gcggcggcaa aatcaattca tcgctctcat
       61 gctgctgggt gtagcgcatc acttccagta cgcgcaaccc cgcacggtgc accgcatcgt
      121 ttaacgcctt ccctttcagc aaaccactga taagctgagc acaaaacagg tcgccagtcc
      181 ctttcaggtc ggtttttacc cgtgaatggg aaatgacatt cacgctgtcg gcactgacca
      241 ccaccacctg catctcctga ttttcttcat taccggaggc gctggtaatc accacccatt
      301 ttaatgtgtc tgaaagcaga ctttttgcgg cagcaatggc actgtcgaga tcgcggcaat
      361 ttttaccggt caggatttcc agctcaaaga tattgggggt aattccttgc gccagcggca
      421 gtaaatattg tcgatacgcc tcgggaaggt caggtttgac ataaattccg ctatcaatat
      481 cgccaatcac cggatcgacc atgatcaata ggtcaggatg gtctttgcgt agcgcagtca
      541 gccactcggc aaggattttg atttgcgatg ccgttcccat atagcccgtg gttacagcac
      601 gaagttggcg cagcgcatca cgctcctgaa gcgcacgcaa atagccgcta aaccattcgt
      661 ccggaatcgc accaccgtag aaagtgtcat aatgcggcgt attgctcagc aataccgtcg
      721 gcacggcaaa gacattcaag ccgttctgtt ttatagcagg caccgcaatg ctgttgccta
      781 cgctgccgta cactacctgc gactgcacgg cgacgatatc cgcctgcagc gccctactct
      841 tatcgttaaa caacaacaaa ctactcattt aattttttct ccttgccgat gatcctcatc
      901 gtattccaac cgaaacttta cctgattctg gcagtcaaat cggctatcac aaaacaagga
      961 taaggtaatt caatgaagaa aatcatttgt ctggtcatta cactactaat gacactcccc
     1021 gcttacgcga agttaactgt tcatgaagaa gcccgcatca acgccatgct ggagggatta
     1081 gcacagaaaa aggatttgat atttgtgcgc aacggtgatg aacatacctg cgatgaagcg
     1141 gtttctcatc tgcgtctgaa gctcggcaat acccgtaacc gcattgacac tgccgagcag
     1201 tttattgata aggttgcttc gtcgtcatcg attaccggga agccgtatat tgtgaagatc
     1261 cccggtaaga gcgatgagaa cgcacagcct tttttacatg ccttaattgc gcagacggat
     1321 aaaacggtgc ctgcgcaata atcttaaatc cccgccccct gactaaaatg ctcttcccca
     1381 aacaccccgg tggaaaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca
     1441 ccagggttag cttttgccac ccgcagtgct ccggcaaccg cgccgccgga gctgacgcca
     1501 cagaatattc cttcccgcac tgccagttcg cgcatggtgt tttctgcatc gcgctgatga
     1561 atatccagca cctcatccac cagagaagcg ttgaaaatcc ccggcagata ttccgcaggc
     1621 cagcggcgaa tgccgggaat actgctgcct tcttccggct gcaggccaac aatcgttact
     1681 ggtttggatt gttcgcgcat aaagcgtgaa acgccggtga tagtgccggt tgttcccatg
     1741 ctggagacaa aatgggtaat gcgcccgccg gtttgctgcc agatttccgg cccggtagtg
     1801 gtgtaatggg cgtaagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca
     1861 cgattcgcca tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca
     1921 agaatcagtt ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg
     1981 ggcatcagca atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg
     2041 gtgttaccac tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc
     2101 gcctcgacga tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct
     2161 tccagtttta accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga
     2221 ggcgtattgc ctattgtttg ttctaatgta ctcacgatct ctatccatac gtggtgttgc
     2281 ctgatgcgac gcttgcgcgt cttatcaggt ctacaggtta caaaccttgc cattaaaaag
     2341 cccggatcgc gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg
     2401 tgcgagagca agttcctcat cgcgggtttc gatacgctcg tcgccgttat acagccgcgc
     2461 atgttgcaga ccaacgaata aacgctcgcc acgctgcggg gcatcgtcgc catgcatcac
     2521 gaccgtcagc ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc
     2581 tttcgggctg gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct
     2641 gatatccact tcccacgggc gcaggaagag atccaccggc ccctgataag caggtgtgta
     2701 gcccagcggc cagcgatgtg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag
     2761 gcggttcact tcgcccataa attcgagcac aaaacgggtc gctggttcgc gccatacctg
     2821 ctccggcgca tcagcctgtt caatattgcc ctggctcatc accactacac gatcagctac
     2881 ttccgtcgct tcttcctggt cgtgggtcac gaacacgctg gtgaatttca gctcttcatg
     2941 cagttgacgc agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccaaacgg
     3001 ttcatcaagc agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacgcgctg
     3061 tttctggccg ccggaaagct gtgccggata acgatccgcc agatgagcaa gctgcaccat
     3121 ctccagcaat tttgtcactt tcgctttgat ggctgcggca ttcgggcgat cgcgacgcgg
     3181 cagcaccgtc aggccaaaag cgatattgtc gaataccgtc atatggcgga acagcgcgta
     3241 atgctggaac acgaaaccga ccttgcgatc gcgcgcgtgc aggcggctga cgtcggtgcc
     3301 gtggaagcga atatgcccgc tggtttgatg ctccagcccg gcgataatgc gcagcagcgt
     3361 ggttttcccg gaaccggacg gccccagcaa cgcgaccatc tgacctgaag gaatatccag
     3421 tgagatatcg ttcagcacct gggtgcgacc aaacgacttc ttaatattgg caatctcaat
     3481 gctcatgatg ttcctcctgc tgtgcgcgtt tttcctgatt ctccaggcgc cactgcaaca
     3541 tactttttaa aaacagggtg ataatcgcca tcagcgttaa cagtgccgca gcggtaaagg
     3601 agccgacggt gttgtagtcc tgctccagca attcaatctg taacggcagc gacagggttt
     3661 cgccgcgaat cgagccggaa accaccgaca ccgcgccaaa ctcgccaatt gcgcgggcgt
     3721 tggtcaacac cacgccataa agcagcgccc agcgaatgtt cggcaatgtg acgcggcgaa
     3781 acatctgcca gccagacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc
     3841 ttaacatcac cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca
     3901 gcaccattcc gggccaggag aacataattt gcagattatg ttcatccagc cagccgccca
     3961 gcgggccgtt agagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacgg
     4021 caaacggaat gtccagtagc gtcagcagta actggcgtcc agggaagtta aagcgcgtca
     4081 ccagccaggc cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca
     4141 tcaccgtcag ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca
     4201 tcagcccttt gctgaacgcc tgtacgaaga tataaatcat cggcaccagc aggatgaacg
     4261 ccgataccag catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt
     4321 cataacgctt caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg
     4381 actttgcaga gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc
     4441 aatcgcgctc gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt
     4501 cacttccgtc ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag
     4561 actacgggta aacgacagcg ccacgcccgc caccagcgcc ggagaaagtt ccggcagcac
     4621 cactttgcag aaactctgcc agcgcgttgc gccaagcgtt tctgccgctt cttcatattc
     4681 cgggcctaac tcttccagca ccggctgcac ggtacgtacc acaaacggaa tgctggtaaa
     4741 ggccatcgcc accgcaatcc ccagccaggt ataggtgact ttgatatcaa acttcgccag
     4801 ccattcaccg taaaaaccgt ttacagaaaa gagcgaggct agcgttaaac cggcgactgc
     4861 cgttggcagc gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg
     4921 atagcgggtt aagatccacg ccatcagcag gccaaaaacg ccgttaaaaa tcgatgccac
     4981 aaacgctgac agcagcgtta ctttgtaggc cgctactacc tgcgggttgg tgatcacctc
     5041 ccagtactgc gcccagctca tctgagccag ctgcatcacc agcgcggaga gcggcagcag
     5101 caaaatcagg cacacaaaca gcagactggt gccgaggctt aaggtaaagc ccggcagcac
     5161 gcgtcttgat gaaactgcaa acattaatta cgccccgccg ctaacagctt gtctaactcg
     5221 ccgccgctgg tgaagtgggt tttcatcact tccggccagg agccaaattt gtcttccacg
     5281 cggaacagct cggtctgcgg gaatttgtct ttcagtttat ccatgacttc cgggttattc
     5341 acgcggtagt aatagtcggt gatgatggtt tgcgcctgcg ggctgtagag ccagttaagg
     5401 taggctttgg cggctttttc cgtaccgttg gcctgcacgt ttttatcaac ccacgccacc
     5461 gggaattccg ccagaatgtt ggttttcgga atcaccactt caaaaccctg cgcttcatac
     5521 tgtttacgga tgttgttcac ttccgactcg aagctgatca gcacatcgcc caggccgcgc
     5581 tcggcaaaag tggtggtcgc gccacgaccg ccagtatcga acacttcaac gtttttcagg
     5641 aactgggtca taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg
     5701 ccccatgccg ccagataggt gtaacgcgcg ttacccgacg ttttcgggtt cgggaaaatc
     5761 agcttcacat cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta
     5821 cgcaccagga agcccatggt ggagtagaat ggcgagctat tatttggcag gcgcgtttgc
     5881 cagtccgccg ggatcagctt gcctttgtca tgcaggattt gtacgtcagt cacctggtta
     5941 taagtgacaa cgtctgcttt taagccctgc aaaattgcca gcgcctgttt tgatgacccg
     6001 gcatgagatt gttttattgt cagtttgtcg ccgccgtttt ctttggtcca ttgttgctca
     6061 aacggcgggt tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt
     6121 tccgttgcct gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt
     6181 ttcagtaagt taacggccat tgcgcaccct tataaattta atgactttct aatagccatc
     6241 atatttataa cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt
     6301 tgaaaaggga ataagaaccg ggtttaaaac gaaatcctcc ccacaaatgc agggaggaaa
     6361 cagaggtgaa tcagataccg acgctaaccg tctccggcag tgtgctgccg ccatcaatca
     6421 cattctgtgt accggttaaa tagctggatt catccgatgc gaggaaggcc gccagttcgc
     6481 cgacttccag cggatcggcg aggcgacgca gcgggattgc tttcgccatt tcagtcagca
     6541 ccgactctgg atcttccggg ttcgactggc gggcaatgct ttccgccatt ggtgtgcgca
     6601 cgtatcccgg gcaaatggcg ttaacgcgaa taccagactg cgcgtactcc accgccagcg
     6661 attttgtcag gccaacaatc gccgctttcg ttaaggcgta cgccgtttcg ccaggatcgg
     6721 ccaccatatc accagtgact gaagacatca tcacaatgcg accatctttg cgggcaatca
     6781 tctccggcag caccgccttc gtgacgttcc atacgccttt aatattgatg tcaatatgga
     6841 aatcgcgatc gtcatcgctc atatcgagga aactgcccag acgacaaacg cctgcgttat
     6901 tcaccaggat atcaatgcgc ccttcttttt ccttcgcgcg tttgatagct gcggctaccg
     6961 acgccgggtc acgcacatcg gcgacaaccg ccgtacagcg atgaccacga ccacacagtt
     7021 cgtccgccag cttttcgatc tcaggggaga tatccagcaa gattaggttt gcgccatgac
     7081 gcgcaaaagt tctggcaatt ccttcgccaa ttccctgcaa tgcgcccgta atcagtgctg
     7141 tcttgcccgt gagtttaccc attttaatgc tctccttgtt aatacagagc gttacactcc
     7201 attccctctg aaaatacagg ataaacaaaa tattgctgcg gatcagctca ctaattcagg
     7261 aaaatctcaa tccagtcgct gctttatgca tcatttcagg cgttgtgtta gctcactgct
     7321 gcgctgaatc attttcaatg actccacatc attgagttgc acaagaccaa caaacagtaa
     7381 atcggtaaca gaattttgcg ccgtgcgggt agacatcgac gagctacgcc attcggtttc
     7441 accggaaacg gtatcgagcg tgaaatgcgc cagccgccgc agcggagagt cagccagaga
     7501 agtaatggca atcaccgttg ccccctgctt tcgtgccgct tctgcgcaca agacaatctc
     7561 ttttttactg ccgctgtaag agatagcgat ttgtacatcg ccttttttca acgcctggga
     7621 aacggtggct tgtacgtgtg tgtcggcttc ataggccacg cgatagccaa ttttcatcag
     7681 tttgaacgac agatcgcgcc ccaccagcgc cgacccgccc aggcccgtta tctggataaa
     7741 cggtgcttta ctgatgacct caatgatttt ttgcagccga gcgtagtcga acaacgcaca
     7801 ggtttgctcc agcgccagct ctttttcgcg attcagtttg cgggcaatca cttccagcga
     7861 atcatcactg gtaatcgaac tgtgcaggtg cagcgccgtg gcattcgttt tctcccggct
     7921 ggcactgtat tcgccaatta acgccatacg caattcagta aatccttgcg cgccgagctt
     7981 ctgggcaaat ttcacaatgc tcgactgact gatgccgagc tgtttcgcca tcttgcgcga
     8041 agagacggat ttcagctcac tcacgttggc ccgcagaaag tcagcaattt tttgctcgtt
     8101 ctcggtaaat tcactttccg cattgcgtat tttcgtcagg tacaacatct tccactctcc
     8161 cgggctggca gcaaaaattc cgcttaagtt cttatgacgc tcttcacact ctgcaagtgt
     8221 agactcaatg attcctttaa gtctgtcaat tcgggatata tgattccata catctgcaat
     8281 tatttgatcg taaatagtaa ggtcaccacc gatgcaactt gaaaagatga ttactgaagg
     8341 ctcgaacacc gcctcggctg aaattgaccg cgtatcgacg ctggaaatgt gccggattat
     8401 caacgatgaa gataaaaccg taccgcttgc cgttgagcgc gtcctgccgg atatcgccgc
     8461 ggcgatcgat gttatccacg cccaggtaag cggcggcggt cgtctgattt atctcggtgc
     8521 gggaacatcc ggtcgtctgg ggattctgga tgccagcgaa tgtccgccca cctacggcgt
     8581 gaaaccgggt ctggtggttg gtttgattgc tggcggcgaa tatgccattc agcacgcggt
     8641 ggaaggcacg gaagatagcc gagaaggcgg cattaacgat ctgaaaaata taaatttaac
     8701 ggcacaggat gtggtggtcg gtattgccgc cagcggtcgt acgccgtatg tgattgccgg
     8761 actggaatac gcccgccagc tcggttgccg cacagtggga atttcctgta atccggggag
     8821 tgccgtttcc accaccgccg agtttgccat tacaccgatt gtaggtgccg aagtggtcac
     8881 tggttcttcg cgaatgaaag caggcacagc gcagaaactg gtgctcaata tgctttccac
     8941 cgggctgatg attaaatccg gcaaagtgtt cggcaacctg atggtcgatg tggtcgccac
     9001 caacgaaaaa ctgcatgtgc gccaggtcaa tatcgttaaa aacgccaccg gatgtaatgc
     9061 cgaacaagcg gaagcggcgt taattgcctg cgagcgcaac tgtaaaacgg ccattgtgat
     9121 ggtgctgaaa aatctcgatg ccgcagaagc taaaaaacgc ctggatcaac acggcggctt
     9181 tattcgtcag gttttagaca aggaataacc catggccaaa gagatcagca gtgaacttct
     9241 gaacaccatt cttacccgtg tcggcggact gggaaatatc gccagttgtg gtaactgtat
     9301 gacgcgcctg cgtctgggtg tacatgacag ttcactggtt gatcccaata tcaaaacgct
     9361 ggaaggcgtg aagggcgtca ttttgaccag cgaccaggtg caggtcgttt ttgggcctgg
     9421 taaagcgcat cgtgccgcaa aagcgatgag cgagctgctg ggcgaggcac cggtacagga
     9481 tgccgcagaa atcgccgccc agaacaaacg tcagttaaaa gccaaacaaa cctccggcgt
     9541 gcaacaattt ctcgccaaat tcgccaccat cttcacgccg ctgatccccg gttttattgc
     9601 cgccggtctg ctgctgggga tagcgacgtt aattgccacg gtgatgcacg ttccggcaga
     9661 tgctcagggc acgctacccg acgcgctgaa ttttatgaag gtgttcagca aaggtttgtt
     9721 cactttcctg gtgattctgg tgggctataa cgccgcccag gcatttggcg gcacgggcgt
     9781 caatggcgca attattgccg cactcttttt gctcggttat aaccccgctg caaccaccgg
     9841 ttactacgcc ggttttcacg atttctttgg tctgcccatc gatccgcgcg gcaatattat
     9901 cggcgtgctg attgccgcgt gggcctgcgc ccgcattgaa ggcatggtgc gccgctttat
     9961 gtcggacgat ctcgacatgc tgctgacctc gttaatcacc ctgctgatca ccgctacgct
    10021 tgcgtacctg atcattatgc cgctgggtgg ctggttattc gaaggtatgt catggctgtt
    10081 tatgcacctg aacagtaatc cgttcggttg tgcggtttta gccgggctgt tcctgatcgc
    10141 cgtggtattt ggcgtgcatc agggctttat tcctgtttac ctcgcgttaa tggacagcca
    10201 gggattcaac agcttatttc ccatcctttc aatggcaggc gcgggccagg tgggcgcggc
    10261 gctggcactc tactggcggg cgcaaccgca cagtgcgcta cgcagtcagg tacgcggggc
    10321 gattattccc ggcctgctgg gcgttggtga accgctgatt tacggtgtca ccctgccccg
    10381 catgaagccg tttgttaccg cctgtttagg cggcgcggcg ggcggtttgt ttatcggcct
    10441 gatagcctga tggggtctgc cgatgggctt aaacagcgcc tttgggccgt ctggtctggt
    10501 agcgctgccg ctgatgactt ccgcacaagg catccttccg gccatggcgg tttatgctgg
    10561 cgggattctg gtggcatggg tttgcgggtt tattttcacc acgctctttg gctgccgtaa
    10621 cgtcaatctg gactgatatc atgaaacgga caatgctcta tctttctctg ctggctgtta
    10681 gttgtagcgt cagcgccgca aaataccctg ttctgacaga aagctcgcca gagaaagcag
    10741 ggtttaacgt cgaacggctt aaccagatgg atcgctggat tagccagcaa attgatgtcg
    10801 gttatcccgg cgtaaacctg ctgatcatta aagataatca gattgtgtat cgcaaagcct
    10861 ggggagcggc gaaaaagtac gatggcagcg tgttgatgga acagcctgtc aaagccacca
    10921 ccgggacgct gtatgacctg gcctcaaaca ccaaaatgta cgccacgaac ttcgccctgc
    10981 aaaagctgat gagcgaaggc aaactgcatc ctgacgatcg gattgcgaaa tatattccgg
    11041 ggtttgccga tagtccaaat gacaccatta aaggcaaaaa caccctgcgg atttctgacc
    11101 tgctgcatca cagcggcggt ttcccggcag atccgcaata cccgaataaa gcggtcgcgg
    11161 gcgcgttata ttcccaggat aaaggccaga cgctggagat gatcaagcgc acgccgctgg
    11221 aatatcagcc cggcagcaaa catatctaca gcgatgtcga ttatatgctg cttggattta
    11281 tcgtcgagtc ggttaccggt cagccgcttg accgctatgt tgaagagtcg atttatcgcc
    11341 cgctcggcct gacacatacg gtatttaacc cgctgctgaa aggctttaaa ccgcaacaaa
    11401 ttgccgccac ggaattgaac ggcaataccc gcgatggcgt gatccatttc ccgaatatcc
    11461 gcacctccac tctctggggt caggtgcacg atgaaaaagc cttttattcg atgggcggcg
    11521 tttccgggca cgcaggtttg ttttccaata ccggcgatat tgcggtgtta atgcaaacga
    11581 tgctgaacgg cgggggctat ggcgatgtgc agctgttcag tgcggaaacg gtgaagatgt
    11641 tcaccaccag ctctaaggaa gatgccactt ttggcctcgg ctggcgcgtg aatggtaatg
    11701 ccaccatgac gccgacgttt ggcacgctgg caagcccgca aacctacggt cacactggct
    11761 ggaccgggac agtaaccgtt atcgatccgg tcaatcatat ggcgattgtg atgttaagca
    11821 acaagcccca ttcgccagtt gccgatccac aaaaaaatcc caatatgttc gaaagcggtc
    11881 agttaccgat tgcaacttat ggttgggtag ttgatcaggt gtatgcggcg ttgaagcaaa
    11941 agtaataaaa aggccagtca gttttgttac tggctggcct ttaatcatcg aatcagaaac
    12001 gcttacaacg ccattaactt gtccagcgac ggcgcgaagt aatagccgcc ggttaccggt
    12061 ttggtgaaac gcaacatcgc atcacgctta ccatcggtat cgccaaacat gctcagcagt
    12121 tgctgctcaa tgttatgcag acgcgcgcag taggcgcaga agtacagacc gtgagtgcca
    12181 ctggcggtgc cgtacggcag gctctggcga acaatcttca gccctttgcc atcttctttc
    12241 agatcaacgc gggtgaggtg agaggtttcc ggacgttcgt cgccgtcaat ctcttcgttg
    12301 gcctctttgg tgcgcccgat catcatctcc tgatcgtgaa cgctcatccg gttgagctgc
    12361 ttcaggttgt gctcccaacg ctgcacaaac acatagctgc cgccggcatc cacgccgtct
    12421 ttgataaccg ccacttcgcg acgcgtctct tcacccgccg ggttttctgt accgtcaaca
    12481 aagccgctca ggtcacgctc ttccacccaa cggaagccgt ggatctcttc tttcacttca
    12541 atgcagtcac caaaggcttc catcgccgcc tgggcgacag agaagtttac gtcgtgacgc
    12601 agagaaagaa tgtggatcaa cacatcgaac tgggtggtcg gcgcaaggcc tttaccgtag
    12661 cccggaaaat ctttcagctc ttctgcccca acgccgccgc tcagagcgcg ccaggtgttg
    12721 ttaccaaagg caaccaccgc accaagatgc gcgtccggga atttcgcttc aaaagttgcc
    12781 agtttgtcgg caaatgtttt actggccgca cgcagggcgt caacttcccc tttcacgttg
    12841 gcttcgatcc aaatcgccgc gcggcaatgt tctggcaaaa tgccactctg aacctgagac
    12901 attgttcctc ctgaaaataa gaatgccacg caatccgtgg cattgttgag cgctatttta
    12961 cgtgtttttt cgcggcggcg gtttgttcag acgcaaatta acgccgccag ataattttac
    13021 tgactttcca gtttttcagg gtatcgtccg aaggcattaa cccttcagga ccacgccatt
    13081 ccccgctgaa ctggtagcta atatgttgac tgccttcggc tttacattcg acggcacgat
    13141 tatcatcacc atcagctttt tggcaattgc caaatgcttt gctgtaaagg tcgctaaacg
    13201 gtgtaccgat tttaacaccg gtgtcagcag gaatatcgct atccagcacg tcaatgcgac
    13261 tgatcgtgcc ttgatcgccg ttaatcacca tcgccacgtt gtcgcctttc atcacttcaa
    13321 aaaagcgcac cacgttgccg ttcgcggttt tcattccgct gcgcaggcga taatcgccat
    13381 caagcgcatc agcaatggct tgttcttgca gtggtgtgga cgccgttaat tcacccacgc
    13441 cctgctcgct cactttggtg gacgatccaa accagttcca cgggttagcg gccgaccagt
    13501 taactgacga catcgtggaa cagccggtca gcatcaacgg catagcgcat aacattaaac
    13561 gcagcgattt catgtcactt cctttggtta ttcaataacg ttgcttggag tgcaaattca
    13621 ccaaaaagtg ccgttattct ttttcttcat taaaacaagc gcgtaaacgt cgattggtca
    13681 gcagccagat taacgccaca atatccgcca ctaccagcgc cagaccgata ccgctaacgg
    13741 attcaccgtt cagccacagc cacggttgcc agcaaagtaa aaccacctgc gccaacagca
    13801 acagaaaata gagcacatgc caagtgcgag gaaatgtagc ccgccgaccg ctcaacaaaa
    13861 acgccagcac cgccggaatg cctggaatca gccccagcca gaaattatcg tgatcgggat
    13921 aaaacagatt tagcagcgca gtaccctgct cgcgcgacgc accggcaatg acaaacagca
    13981 cccaggttcg cgcctgaagc aatagcacaa gccagaagag caagggtaaa cgcaggcgtc
    14041 cgtgcgcatc ataatggaca ggatggaact cagtactctt catcttcaat caaacgctta
    14101 cccagactca gcacgtcggc gtgttcatat cccaggcgtt catacattcc gagcaccatg
    14161 tcgttatctt ccggcacatt gatctgaatt ttcgggcagc cacgagcaat cagctttttc
    14221 tccagccgat taagcaacgc attggcaatc ccacgcccac gaaactctgg atgcacgcca
    14281 agataataag cagacccgcg atgcccgtca taaccgccca tcaccgttcc gaccacttca
    14341 ccgtttacct cagcgaccaa aaacaaactg acgtcatggt tcatcttacg ctcgatgtcc
    14401 atttccggat cgttccacgg acgcagcaag tcgcaacgct cccaaagggt gatgacctct
    14461 tcgaaatctt cctggcgaaa tacgcgtatc tccatggtat tcgttacctt tttgcgggtt
    14521 aaaaggctga ttatggcgtg aacggtcgaa ttagccaata tctgacgaaa atcggttgaa
    14581 aaagtggcat aatggggagt tgtcaactat tgaaatgaaa agtaaaacaa ttctcaacat
    14641 caaaccgtcg taacggatta cgcgatacga tataacatct ggaactttat tattacaact
    14701 caggccgtat gagcactttt aaaccactaa aaacactcac ttcgcgccgc caggtgctga
    14761 aagccggttt ggctgccctg acgttgccag gaatgtcgca agccatcgcc aaagacgaac
    14821 ctttaaaaac cagcaacgga cacagcaagc cgaaagccaa aaaatctggc ggcaaacgtg
    14881 tcgttgttct cgatccaggc cacggcggaa ttgataccgg agcgatcgga cgcaacggtt
    14941 cgaaagaaaa acatgtggtg ctggcgattg ctaaaaacgt ccgttccatt ttgcgtaatc
    15001 atgggattga tgcgcgttta acgcgttctg gcgatacgtt tatcccactt tacgatcgcg
    15061 ttgaaatcgc ccataaacat ggcgcagatc tgtttatgtc aattcatgcc gatggcttta
    15121 ccaacccgaa agctgccggt gcttcggtat ttgccctctc taaccgtggg gcaagtagcg
    15181 caatggcgaa atacctgtct gaacgcgaaa accgcgccga tgaagttgcc ggtaaaaagg
    15241 cgactgacaa ggatcaccta ttgcagcaag tgctgtttga tctggtgcaa accgatacta
    15301 ttaaaaacag cctgacgctt ggctcgcata ttctgaaaaa gattaagccg gtgcataaac
    15361 tgcacagccg caacaccgaa caagccgcgt ttgtggtgtt gaaatcgccg tcggttcctt
    15421 cggtgctggt ggaaacctcg tttatcacca acccggaaga agagcggctg ttaggcacgg
    15481 cggcgtttcg tcagaaaatc gccacagcga ttgctgaagg cgtgatcagt tatttccact
    15541 ggttcgacaa ccagaaagca cattcgaaaa agcgataagt tatgaaaccc gacgcacacc
    15601 aggttaaaca gtttctgctc aaccttcagg atacgatttg tcagcagctg accgccgtcg
    15661 atggcgcaga atttgtcgaa gatagttggc agcgcgaagc tggcggcggc gggcgtagtc
    15721 gggtgttgcg taatggtggt gttttcgaac aggcaggcgt caacttttct cacgtccacg
    15781 gcgaggcgat gcctgcttcc gccaccgctc atcgcccgga acttgccggg cgcagtttcg
    15841 aggcgatggg cgtttcactg gtagtgcatc cgcataaccc gtatgttccc accagccacg
    15901 cgaatgtgcg tttttttatt gccgaaaaac cgggtgcaga gcccgtctgg tggtttggcg
    15961 gcggcttcga tttaacccct ttctatggtt ttgaagaaga cgccattcac tggcaccgca
    16021 ccgcccgtga cctgtgcctg ccatttggtg aagacgttta tccccgttac aaaaagtggt
    16081 gcgacgatta cttctacctc aaacatcgca acgaacagcg cggtattggc gggctgttct
    16141 ttgatgatct gaacacgcca gatttcgacc actgttttgc ctttatgcag gcggtaggca
    16201 aaggctacac cgacgcttat ttaccaattg tagagcgacg taaagcgatg gcctacggcg
    16261 agcgcgagcg caattttcag ctctaccgtc gcggtcgtta tgtcgagttc aatctggtct
    16321 gggatcgcgg cactctgttt ggcctgcaaa ctggcgggcg caccgagtcg atcctgatgt
    16381 caatgccgcc actggtacgc tgggagtatg attatcagcc aaaagatggc agcccagaag
    16441 cggcgttaag tgagtttatt aaggtcaggg attgggtgta actccctcac ccccattccc
    16501 gcatccgctg atgcaacgtc aacgacggct tctcggcaaa cagctgctgg taatccgtgg
    16561 caaattgccc cagatgccag aatccccact gcatggcggc gtcttttacc gttgtgcttt
    16621 gcgaccacgg gcttatcagt tcgcggcgta cggcgttcag gcgaatgcgt ttcagccacg
    16681 cgtttgggcc aatgcctaaa atagcgtgaa acgcgttttg tagcgtgcgg cgactgacat
    16741 gcagttgatt acacaagtcc agcaccgtca ccagctcaga catattttcc agcacatatt
    16801 cacgggcgcg ggaaagtaat ctacggtaac tctgatgact gatgctttcc gccgtcacca
    16861 ttggttgcgc gtcttccagc atcgccccca tcgccattag caaattatcc cccagcactt
    16921 ttcgcactgc aggctgatgg agattttccg ggttctcgca aaacgtcgcc agcgcctgtt
    16981 ggacaaagcc ccacagcgcg gctttatgct gctctttcac ttccagcgcc gactggctac
    17041 gcagcatatg taatacccga tccggattgt gcaaaaagtt agcctgacgg gtgatgacat
    17101 cttcagacag cactacgccc aggatcgtat aatcgtccgg cgtacttaat tcaaactcgg
    17161 ttccaccggg gcgcgtcgct atttctgcgc ttcccagaca ttgcgaaccg ataaatccct
    17221 gctcaccgcg cgtcgccgga atgccaaacc agaacgagtt cggccagacc aggcacgact
    17281 gacgcagcgc cagaccggtg tattcacgaa aaacctgaat atcatcgagc agaatttccg
    17341 taaattcacc atgaaacttg cccggatgca gctgatcgta aatctgctgc caggcggtaa
    17401 tcgtcaaagc atgttcatag acatccgttg tctgtcgttg atgaacatta tccacttcga
    17461 ccttcggcgt gagcttcagg ttttcgggta agggttcatg ataaagatgg tgcaaattgg
    17521 ctgtacgggt ctttttcatg atgttaatgc cgggtgttgt aggacacccg gcacctccga
    17581 caggttaatg gggcttgaga cgataacgac tactgcgttt acgtaacgtc ccggcagaaa
    17641 agagctgttc gagcgcattt ctggcttttt ccagcggcca gccaaagtgg gcagccactt
    17701 ctcctgccgt cattccctga cgtactgatg ttaacagcgc caacaactcg tcagcagatt
    17761 ccgcgacgat aactggcgcg tcgggttcct ttacgggttg cttcggctgg cggttaaagc
    17821 cagtaaccag ccactgggtg tcatcgtctg gccgaccgat ctccttgcgg ctgatgacac
    17881 gcccggtacg catcgcggca gcacaacctg cgtccagcgc cgcacgacac gccgccagat
    17941 cgccttccac caccagcgtt aagcgaccag ggtcaagcac ttcgtgactg agcagacgaa
    18001 cgttagctgc tttgagcatg gcatccgctg catctatcgc ggcgaccatg ccgtccactt
    18061 ccagcaatcc cagtgcattg atcatcggca acctccgtta cgcacgctgg attggattac
    18121 gcgcgatttc cagcactgca tcggtaaagg cgttacaggc tgctttacac gcggcctggc
    18181 taccggttaa aaatgctgcc gagtagttgg tttcagacgg tggcgggaca taggtcacca
    18241 gctgtacgtc ggcagatttc aacgctgcat caatgccgta ggtcgcttcc agcggcggtg
    18301 ccaccagata cgccatcgga tcgccaagcg tgatcccggc ggttgatgaa agataagaac
    18361 cggtacgcga aactacatgt gccaggaatg cggtgttttc tgcgtcatta gcccactgga
    18421 aagcagcgcc attttcaata tgcgcaacca tcgcatccag accagcacgc acttccgccg
    18481 ggtttggccc accaagcata atcagcactt caccggcagt cggtgacggt ccgtgtgctg
    18541 ctcccgcata cagcgagcgg ccatatacca cttcgaccat cgcctgcttg gtcgcttcgt
    18601 cagccgcaat gtaggtgacg tcatcggaat cagcagaaat cagcccgagg ctacgaatat
    18661 gcggcggcaa ttttagttca cgcgcaaatt cagcgttaac ggaggcaatc acccgcatgg
    18721 cggttaccga cggtcgaatc aaatctaaag ctggcatgat gcctccttat cgggtcatgt
    18781 tgatgccgga tgctttctgc tccagcatac gtttcgccaa atccacaatg acggctgcgg
    18841 cttcaaccgg cggcgtgccg ccctggtgaa tgttagagat gcaggtgcga tcggcctcaa
    18901 cggtggtcgc catacgcggc gagtaaacgg cgtaacagga gaggctttct gactgaccca
    18961 accccggacg ttcccccacc agcaggatca ccactttcgc gccgaggatc tcaccgatct
    19021 gatcttcaat cttcacgcga ccataacgaa caaagaatgg cgtgccgact ttcagcccgg
    19081 cctgtttcag gcccgccatc agcggcggca ggatctcttc gtagttcacg gtgatggcat
    19141 cagttgacag gccatcagaa ataacgacct gtacgtccgg attagcaaca cactgtgctt
    19201 tcagcgcttc aacagcttct gcacacaggc ggcggcccat atccgggcga gtcaggtaga
    19261 gatttttgtc gctaatttcg gagcgtactt ccagcaagcc ctgcgctttc acccactctt
    19321 ccggcacttc tttcagtacg gtatctttcg aacgagagtg atcggccagg aaacgcagca
    19381 gcgcctgcgt acgtggacgc ggaccggtac gaccggtaca aacgcgggcc acggtgctgc
    19441 ggcgcagttc tgttaatacg tctgcgcgat gcgggttttc aacaccaatc cacgcttttg
    19501 cttccgcaga acctaaatcc agcgcgcagc tttccgaggt caccggtgcc gcacagttag
    19561 tggtggcgca ctttgcttct gacggcgcgg gggccgcttg tcccattgac gccatcacgc
    19621 tgcgtacaat ttcttcaatc tgtttttgat ccatgatgtg ttatctccgc gtcatcagaa
    19681 gaacagtgac ggatcgcccg cccgtttggt caggcgaccg tttgccataa tgcccatgct
    19741 ttccagccag cgttcaaact ccggtgacgg gcgcaggttg agtaactgac gcacagtggc
    19801 agtgtcgtgg aatgcggtgg tctgatagtt gagcatgatg tcatcgccca gcggcatccc
    19861 catgatgtag ttgcagcctg cggtggcgag caggatcatc aggttttcgt tgaggttctg
    19921 gtcagcgtca gcgtggttgg tgtaacagca gtcacagccc atagagatgc cgctcagctt
    19981 gcccataaag tgatcttcta agccagcacg gataatctgg cggtcgttgt agagatactc
    20041 cggcccaata aagccgacca cggtgttgac gataaacgga tcgtaatgac gcgccagccc
    20101 atagttacgt gcttccatcg tcacctggtc tgcgccgaag ttagcgccag cagacagcgc
    20161 cgaaccttgc ccggtttcga agtagaggca gttttccccg gcgatacggt tgaactcagc
    20221 acccactgcg cgcgcttcgt cgagcatcgc cagttccacg ccaaactctt tcagcccttt
    20281 ttcgctgcca cagatactct ggaaaatcag tccgccaggc gcgccgcgac gaatcgcttc
    20341 gatctgggtg gtgacgtgcg ccagtacgca gccctgagtt gggatgttga atttgtcgat
    20401 cacgccgtaa atggtgtcca acacgcggct taagttttcc acgtcgtcag ttaccgggtt
    20461 aacgccgatc accgcatcgc ccaccccgaa ggaaagccct tcgtagattt gtgcagcgat
    20521 actttgcacg tcgtcacggg tgtcatttgg ctgcaaacgg gcgctgaagg tgcccggaat
    20581 accgatggtg gtattggcct ttttgattac cggcattttc ttcgcgccgt agatcaggtc
    20641 cgcgttggag caaatcttcg ctaccgccgc gaccacttcc gaggtcagcc ctttgcgggt
    20701 aaaggcaatg tcgtccacgc tggtttcatc gctcagcaca tactcacgca gttcgctgat
    20761 gctcctgttt ttaatctggt tgtaggccgt ttcgttaaca tcgtcctgaa tcagccgcgt
    20821 cacgcagtca tcttcatagg caatcaccgg attattgcgg atgtccgcta cggtcatttc
    20881 cgacaacacc tgctttgccg ccacgcgctc ctgtgagctt gccgctgcaa cgcccgccag
    20941 cacatccccc gaacgcagtt cgttggcttt agccagcacc tcttttacat ccttaaactg
    21001 atatacattg ccgaacaatg tggtctttag tttcataagt cgttccctca ggaaggaaat
    21061 gcgagtgatt tcaccgtcac cggcacaacc gatccgccaa aaagaggcgt accaatgtcg
    21121 atatagtccc ccgcgcggac aatcacttcg tcaatgactg ccaacgggag ttgttgtagc
    21181 tgtgggcgca acaacatgcc cagagcttta ccaaagtcct gcccggccac caccagcagg
    21241 ggatgcggat ttggaaaacg cgcgacgaaa tcgaccagcg cgttgatgac cgtcagtacc
    21301 gcggcgtaac gcacaggcag cgaggcggga agcgccagca cgtacgcgtc agttttggga
    21361 tcaagatcca gctgaagcag cgcctgttgc caggcactca ccagatccgt ttcatcaatc
    21421 gggatcgcca ccggcaaatt gcgcagcggc agttgtacgc cctcaagcca gattgtgctg
    21481 ccagagagcg aaagggtatg cgcgcccgcg ccaatcaccg tggcgcgcac ggtttgcgcc
    21541 ggaaactgta cgttcatctc acgcaggcgc ggatggtcat gcagcgccgt tgccagcagc
    21601 gggccaatat cggcaaaaca gaacgggtcg gcgggctggt ggcgataaca ttcgcccacg
    21661 ccgccagaaa gcgtaatgat ttcgggcgta acacctgcgg gcagcaaacc ggtttgcatc
    21721 aatgcctgcg cgagcggcga gagcgttccg tcaatcactt cgacaatcag ttctgccatc
    21781 cgccgggtaa cctgcaccag ctgcgcgccg gtcagcgaac gggcgtcggt gcctgcgccg
    21841 aagcactcat ccacaatcat ctgccccggt ttatgagcgt aaaccacgcg cccctggctg
    21901 tcggtttcca gcaggcgacc accgacgttg aggcaggcag tgccgctgat ttttccggca
    21961 tcgaacaggg cgtagttcgc agtgccaccg ccgatgtcaa tattcagtac ccgacacagc
    22021 cgttgttcag aaagggtttg tgccccggca ccgtgaccgg caatgacgga ttcgaggtgc
    22081 ggcccggcgc tggcaacgac aaaatcgccc agcgactggg agagcaccat caccgccgga
    22141 cgagcattgc gggttttcgc gctttcaccg gtgatgatga tggcaccaga atcaacgctt
    22201 tccggcgcaa tgcccgcagc ctgatattgc tcgaggatta aggttttcag ttccgcttct
    22261 tttaaaccac cctgtttatc gacaggggta aagaacaccg ggctttgcca gctaatttcg
    22321 cgtttaatga attcgtagcg cggcacctgc gacaccgccg cacggttaac cagctccagg
    22381 tgggagaaaa tcacctgggt ggtggtggtg ccgatatcga taccgacgct caatagctgg
    22441 cgagtgttca cgattgtgcc tccgcttcgg ttttagccgt ggtcgcgtct tcttttggca
    22501 ccagcatcat cgccacgcca atcgccgtta cgccgccgat caacttgccg acaatcatcg
    22561 ggaagatcat ggcgttcatg ttggcagcgg cgaagcctaa gtggtcgccc agggcgaaag
    22621 cagcggaaac ggcgaaggcg cagttgatga ctttgccgcg ggtatccatc tgcttcatca
    22681 tgccgaacat cgggatgttg ttggcaagcg ttgccaccat gccggctgcc gcgatgttgt
    22741 tcatattcag cactttaccg acgctcatca gcggtttttc aaaccagcgg gtcagcagca
    22801 gcaccattgg atacgcccct aacagaacgc aggagataga accgataact tcaatggcgc
    22861 gcatcacttc accgggttta tcgccagggg ccataaagat agggtccaga ccggggatca
    22921 gttcccagcc aagcagaaac ttaactaccg cggcagcaag accgagggtg atcaatgcaa
    22981 cgaggaattt ggcgaagatc tggaagccgt tgatcatttt ttccgggatg aatttcagcc
    23041 ccagcgccac cagaatcgca acaatgatca ccgggatcat gttcatcagg atcagggcga
    23101 aagtgaattc caccggctga ccgttgatct gcacaccgga gtacatagca accagaccac
    23161 cagcaataca gccaatcgga atggtcacaa tgcccgccag cacgccgagc gccagataac
    23221 gacggtcaga aggttcgata atgccgagcg ccaccggaat ggaaaacaca atcgttggcc
    23281 ccatcatcga cccaagaatt aacccagagt atagccacgc tgctacgtcg ccgcccgcca
    23341 gctctttggc gaggaagaag ccgcccatat cgcacgccag cagcgttccg gcgaacattg
    23401 acgggtttgc gccgagcatt tcgtaaaccg ggataattac cggcccgagt acgtgagcca
    23461 gtaccggtgc cagcgcggtc ataccgacca tcgccagacc cagagcgccc attgccataa
    23521 agccttcttc gaactgaccg cctgatcctt cgatactttt accgaactta ccgaggaaac
    23581 gagcagaacc gccgaactgc gacaggatcc tgtctacggc agctatcagc ataaagaaca
    23641 tcatgatgta catgatgatt tcgttaattc ccatcgcctt tactccctgt tagttgttat
    23701 ttattggcgg atgcggcgta aacgccttat ccgccctaca tgtgcaatcc cgaaggccgg
    23761 atcagacgcg gcaagcgtcg catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg
    23821 aacgccttat ccgccctaca tgtgcaatcc cgtaggccgg ataagacgcg gcaagcatcg
    23881 catccggcaa ttgcaccgcg ccactggcgg atgcggcgtg aacgccttat ccgccctaca
    23941 tgtgcaatcc cgtaggctgg ataagacgcg gcaagcgtcg catccggcat ttattgcgcc
    24001 gccgcgtaca gtccgacaat ctgttccagg ctggcggtac gcgggttact gcgcagacaa
    24061 atatcttcca gcgcggcctg cgcccatgcg ccgtaatgcg cagatgtcgc accaacatcg
    24121 cccagtcgtt taccaatccc aacttccgca atcagctcac ttaccgcgtt aatagcgtca
    24181 cgatcgtcgg attttttagt tcgcagtgcc cgaccaatct gactaaagcg ttcacgacaa
    24241 accatccggt taaattccat caccgttggc agcaacatgg cgttcgccag accgtgtgga
    24301 atatgcagcg ccgcgcccgg ctgatgcgcc atcgcgtggc acaacccaag acccgcactg
    24361 gaaaacgcca ttcccgccat acatgaagcc agcaacatgc tctcgcgcgc ggcaaggtcg
    24421 tgaccgtagc ccaccgcttt cggcagcgat ttgccaatca tcgcaatggc accaatcgcc
    24481 agactgtcgg taaacggtgt agcgttcagg gcgctgtatg cttcaatggc atgggttaac
    24541 gcatcaatgc cggtcatcgc cgtgacatgc gacggcacac cttcggtcaa cgcggcgtcg
    24601 agaatcgcca catccggcat cagcgaggca tgggctaaca cctgcttgcg cccgctcacc
    24661 gcgtcgataa tcaccgttac attggtggtt tcagagccgg ttccggcggt cgttggaatg
    24721 gcaatcagcg gcaagcgcgg ttgcagaacg ctggtttctg acatctctgc cagcgtgcta
    24781 tccgggttcg tcaccagcaa cgccacggct ttcgccgcat ccagcaccga gccgccgcca
    24841 aatgcgatca ccccatcaca gcctgactca cgcaactgcg ccacggctgc acacacgtcg
    24901 gtgatgcacg gttcgcccac cggacatggc cagagcgtca tggcgatgcc tttaacagcc
    24961 aggctgcgcg tcagcccggc ggtcatccct gcctgatgca aaaagctgtc tgccatcacg
    25021 aacagatgtt tcagcccacg cgtttgcgct tgctgtccgc aactgctcac cgagcccgga
    25081 ccgcaaagcg tcaccggtgg aacgctaaat gtttttaccc gttgcagatt cagggtatcg
    25141 aacgcctgaa agagcgcggt ctgcaattca ttttgcatat agcccctccg ctttctctcg
    25201 cccactactg gcgatcgcca gcggggtcat aaacaaactg tgctgcggta aatgcacctg
    25261 taacgccggg aattgtttgc gaaacagttc cgccacgccc ggttgcatac aagagccgcc
    25321 cgccagccat aaatcagtaa tgccctgccc ttcaatatgc cgggcgacaa tgtccgccat
    25381 tttttcgtac accggtttca ccgtaggcca aatctcttca ccgtgaccgc gcttgtactg
    25441 ctccgcctct tccagcgaaa tacggcgatt tccggcgaga gtcagagaga tgtgatgtcc
    25501 gccggttgct tcatccgccg agtacgtcac cttgcccttt ttcacgatgg caatgccggt
    25561 agtgccgccg ccgatatcca ctacaccggc gttgtccagt tgcagcagat ccgcaactgc
    25621 ggttggctca tccagcacat ggctcacttc cagtccagca gactccagca cgttgatgga
    25681 aatacgcggg tccgtacctg gaggaaatga agtcgccgca tggctaaaac ggcgaccgaa
    25741 ttgctgctcg agcgtgtcga gatggcgacg aacaatggtg acagcgccga agaaatccca
    25801 gacgatgccg tcgcggacca catcggccca gtcgaggcat accgccaccg actgaccgtc
    25861 gcggtcgaca accatcgaca ccacatcgca ggtgcccaga tccaccccca gccacagcgg
    25921 agattccgtc gcggcgggcg tctggttaca cagcgttgcc gccgtttgca gacgtggggt
    25981 gagccattgt tcgtcgtgcg ccatctgtta ctccttaaac aatgcgaaac gcatcgacta
    26041 atacacagcg acgcagacgg acaaacgtac gcacgctagt taccccttca ccggttggcg
    26101 tggtgatggt catggtggtc cagccttccc cgcccagccc cagcccggca atgcgcggtc
    26161 cgttcttaac gaaaatgctg gtatcaatag cattcgccat ctggttcatg ttttcgatgt
    26221 tgcgcgagtg cattgccgcc gtgtggtggc aaccgccttc cagtttcacc gccagcgcaa
    26281 tggcatccgc cacgttggcg acgcgcacga cgggcagcac cggcatcatc agttcggtca
    26341 cggcaaacgg atgttctgca gtggtttcca caaacagcaa acgcgtttct tgcggcacgt
    26401 tcaggccgat tgccgccgcg attttcgccg catcgcgacc aacccagtca cggctgacgg
    26461 tgccttttcc gcgctcgtcg atatttttca gcaacaccgg ttgcagctgc tgcgcctgtt
    26521 ctgcggtcag tttcaccgcg tgctggcctt ccatcagacg catcagttca tcggctacgc
    26581 tatcaacaac aatcagtacc ttttcgtcgg cacaaatgat gttgttatcg aaagaagcgc
    26641 ctttgacgat ggactgagcg gcacgggcga ggtcggcggt ttcatccacc actaccggcg
    26701 ggttgccagc gcctgcggca atcagacgtt tattggtgtg tttacgcgcc gcttctacta
    26761 ccgcttcgcc gccggtcacc accagaaggc cgatgcccgg aaacttgaac aaacgctgcg
    26821 cggtttcgat atccggattt gccacagtaa ccagtaagtt ttccggcccg cctgcggcaa
    26881 caatcgcctg gttgagcagc gtgatcgccc gctgggagac ttttttcgcc gccggatgcg
    26941 gggcaaaaat gacgctgttg cccgcggcaa tcaggctgat ggcgttatta attacggttg
    27001 ccgccgggtt agtggacgga gttaccgaag ccaccacgcc ccagggcgcg ttttcaatta
    27061 aagtcaggcc gttgtcgccg gtcagcacct gcggagagag acactcaacg cctggcgtgc
    27121 cgcgcgcctg agcgacgttt ttggcaaatt tatcttcaac gcggcccatg ccggtttcac
    27181 tgacggcaag ttccgctaaa tctctggcgt gtttttcgcc tgcctcacga atggcagcaa
    27241 tggctaactg gcgcattgcc acgcttttta acccttgctg ggcgactttg gctgccgcaa
    27301 cggcgtcatc cagggacgcg aaaacgccca tctcatgaac ggcggcgggc ggcgtgtcac
    27361 tgctttgcat tttcagcagt accgctttca ccacctgttc aatatcctgt tgattcatga
    27421 tgttctgcct tatttgtgga aaattacctg accgccagac accacctcat cgacaatgcc
    27481 aatcacgcac agatcgaccg gtgacgtttc gcttttatgc gcctggcggg cggaactgcc
    27541 actcaccagt aacacccact ccccggttcc cgcgccaata ttgtcgatgg cgacggcgca
    27601 ttgcccgtca ggattacctt gtggatcaat catttccacc atcagcaatt tgtcatgcgc
    27661 cagtccgtga tggcgtacgg tacaaacaat ttgtccagtg acgactgcca gtttcatacc
    27721 cgcctccgtg gcgtatttca ggtaaaagct ccccctaccc tccgcagaag gtaaaatgaa
    27781 aaaggagaga gcgtgacgcc cgaatcgacg tcacacaggg tgattacagg ttgctgctat
    27841 cgcctttcag gccgatcggg aagacttctt ccaggtcgcc gtgcggacgc gggataacgt
    27901 gtacagatac cagctcgccg atacgctgtg ccgctgcggc acctgcatcg gtcgcggctt
    27961 tacaggctgc aacatcacca cgaaccatgg cagtacacag gccgccgcca atctgcttaa
    28021 caccaaccag cttgacgcgc gcagctttaa ccatcgcgtc agaagcctca atcagtgcaa
    28081 ccaggccccg ggtttcgatc attcctaatg cttccattgt gctttcctct ttatcagggt
    28141 ccagaacggg accgttcatt caaccagtgt ttgtaaactg ctttcgcggt tcacttctgt
    28201 ctgacgcggc acagctgcca ccagcgccag ctcgataatt tcctgcacgc tacaaccacg
    28261 agagagatcg tgcatcggcg cggcaagtcc ttgtatcaat ggcccgacgg cacgatatcc
    28321 gccgagtcgt tgtgcgattt tgtagccaat atttccggct tccagcgacg gaaaaaccat
    28381 cacattggct ttgccctgta gcgggctggc aggcgctttt tgcgccgcca cttccggcac
    28441 gaaggcggcg tcaaactgca actcgccatc aaccaccagc tttggtgcgc gctcacggac
    28501 gatttctgtc gcctgctgga cgttggcaac gcaggggtga cgggcgctac cgttactgga
    28561 aaacgacagc atcgccacgc gcggctcttc tccggtgagg gcgcgccagg tttcggcact
    28621 ggcaagcgcg atatccgcca gttgcgccgc cgtcggctgt ggcaccacgc tgcaatcggc
    28681 aaagcccaac gccggaccgc tgtactgtgg cagcatcagg aaaatggatg agagcgtttt
    28741 acagcctggc tgcaagccga taatacgtaa tccggcacgc agcacattcg ccgtggaaga
    28801 gaggttgccc gcgatacaga catccgcttt accggcgctg accattgctg cggcgaacat
    28861 cagcggatcg gtaagttttt ccagcgcatc cggcggcgtt ttttcgcccg cgcgggccag
    28921 ccagcgatga gcaaattctt cccgcattgc gaggttgcca tgcggatcaa tcacctgtag
    28981 cccgtccatc gccacgccgt gactgagcgc aaactgacga agttcaaacg gattagcgac
    29041 cagaatgggc gttgccagac cttgttgatg taaatattgc gcagctttca gcacacgttg
    29101 atctaacgca tccggaaaaa ccactctggc gggcgctcgc aacgccagtt cacgacaacg
    29161 ttcaataatc attgcgtttc ccccagtcgt tgttggatct gcttcaccgt tagcggcggc
    29221 ttcgttacgc tcaggatcat cattacgtag accgtgctcg aaaggcggtt cagcgcctgc
    29281 aagatgtccg ggcgtaatac ttcaaagctg cgggtgataa acacctgcgc cgctacggtt
    29341 tcggtttccc gcactttggt acgtagcaga ttgagcaacg ccgcatcgcg cccgtggctg
    29401 gcttccggca ccagatgatc gtggtcgaga tagcgcagcg gctggtgaga aagtcgatgc
    29461 agatcttcgt cagaaagccc gacaatcgcc tggcagccca gcggttcccc cagcgcatcg
    29521 gcgcgcataa tgttgccaag acgcgaacgg atatccgcca gccacggctg ccacggttcc
    29581 gccagttcaa tttgcagcca cactgccagc gcaatggtgc tgtcgagaac cgcgcgaaaa
    29641 cccagacgcg gatcgctttt ggcgaccatt ttttccgccg acaggtgagt cagcgtatcc
    29701 ggcttttttg ccaccggctg gcggcacagt tcgcagcacg cctgcggatg ttcatcgcta
    29761 ctggtcagcc catgaacggg ctgcggctgc tgctgttcat cgtcaacaaa caggcggccc
    29821 tgctcgtcaa taaacttgat gcgcagatgg cggctttcca gcaactcccg ggcagagggc
    29881 gtcaggcgac tgtccgcagg gagatggatc tctgcgcctt cgctgagcgt atggttcgct
    29941 cttagccatg cttcggtgat gaaatctttc atagggattg ccagttagcc ggccaggcga
    30001 catacaggaa tttcacgctg gatgtcgtac caaattcgat gctggagcct ttcgggataa
    30061 acatcacgtc gcctgctttg gcgatcatgg tttcgccttc gtggcgaaca tggagctccc
    30121 cttccagcac catgtcgatt tcgtcgtagt tcagggtcca cgggaagaat gcgttctccc
    30181 actgcataaa accggcggcc atgctgctgc cgtcgtctcc ggtcacaaga tcagtcaagc
    30241 ccacgcaatg cggttgtgcg ccatcaaaac gaccaaactt gacgctgctg ccatcaatca
    30301 ctttgatgcc gcctttgccg gtcaccgatt tgaagctcgg ctgcattgcg ccctgctcca
    30361 gcgactgttt ttccttcatc actttttcca tcagctgtgc gaccaggctt tcggtaaact
    30421 ggccttccgg cagctgggca atgatggttt cgcggatgcg ctggctttcg gttttgtcgg
    30481 ctggcacgct ggcgggcacg gacgccgtta ccgggatcga ttcatcacat tcggtgatgg
    30541 taaaacccag tagatccgcc actttacggg cttccggggt aataatgctg gcgcgcagaa
    30601 ccacggacat tgcctgttcg ccgcgcgcgt gtgcttcacg aatatcattc gctgtgatga
    30661 gttttttcac ctgcttcctc ctgtttggtg agagatgcca gataatccac cagttgctgc
    30721 acgctttgcg ggtcgtggct gtttagctcg aaaatcggct cttcgaaacc cgtctcaagc
    30781 aataactttc gcgttgcggc gacgtcagca tctggcatgt ccgttttact gatgacggcg
    30841 atttgccgtt tgctgacgcc aatatccagc aacccggcag gcaaccgact ttccgggtca
    30901 tttgcgccat ggacatagat cagcatgtct acatcctgta gcgtggtaat taaggcgtga
    30961 taccagcggg gatggctaaa atattcaccc ggcgtatcaa tatcgccctt atcattaaat
    31021 tccacggcct gtgtttttct ggcgagggta taattcccct gcaacgcatt aaataacgtt
    31081 gtttttcctg ccccgacgct acccacaaac gcaattcgtt tcatgaatgc ctcgtattaa
    31141 cttttggtca tttcacaaag cgtataattt aataagcgac ctaaaccgct gacggtttgt
    31201 gataacgctt cttctactgc gcccaccgag ccataaatca ccaatgcacc gctgaagcgg
    31261 tcgagaaaac cgatatgcac atcagcggct ttcagcgcta aatcaccgac gatcatcgcc
    31321 gtttcgccgg gagttagcgt cataatgcca attgcgcccg catccggaac gccgattttc
    31381 ttcgccagtt cctcaccggg atgcgcgatg agatgcgcca gtgtgacctg tttgccgggc
    31441 acaaattcct gaatgatgcg ttctttatcc atgagtcgcc gcctgtgaaa aactttcgtg
    31501 tatcgtgaca atttttcgtc agagtaaata acaaaattcg gcaagcgcgt ttaaaaggtg
    31561 aggtagatca ctaagaatgg agagatattc gctgtggtgc ataaactttc atatgcaacg
    31621 catgaatata aataaaataa tatcgagata ttatatagac tgcttttttt tattattcgc
    31681 aaactcagta ataagcttta ctaatatatt gcagccaaac cgcttaccct gaatattcag
    31741 ggtaagcgtg agagttaaaa aagaattaca gcggttgggt ttgcgcttca accacggcca
    31801 gcgccaccat gttgacgata cgacgcaccg atgcgatcgg cgttaacacg tgaaccggtt
    31861 tcgccacgcc catcagcacc ggaccgacag tcacaccttc cgagctggaa acgcgcagta
    31921 agttgtaact aatgcgggca gcttccatgt tcggcatcac cagaatattg gcagaacctt
    31981 tcaaagggct gtccggcata cggtcgttgc gaatcgcttc caccagcgcc gcatcgccgt
    32041 gcatttcacc atcaatcatc agttctggcg cacgttccct gaccagttcc agcgcctgac
    32101 gcattttgct cgacgacggg cagtcagaag aaccaaagtt ggagtgcgac aacagagcaa
    32161 cgcgcggttc aataccgaaa cgacggacag tttctgccgc catcaaggtg atctccgcca
    32221 gctcttctgc gtctggttcg tcattgacgt aggtatcggc aataaaggtg ttaccactcg
    32281 gcagcagcag cgcgttcatt gcacctgcgg tgtgaacgcc atcgcgataa ccaaagacat
    32341 ttttcaccac gctaaaatgt tcatgataat cacccaccgt accgcaaatc attgcatcgg
    32401 cttcgccacg ctgaaccatg atcgcgccga tcactgtcgg gttactgatc agcgcccgct
    32461 gcgcctgttc ctgagtgacg ccgcgacgct tcatgatctg gaagtattcg gtccagtact
    32521 ctttaaagcg cggatcggat tcgttattga cgatctcaaa atcaacgccc gctttgatct
    32581 gcaagcccag tttctgaatg cgcatttcga tcacgttcgg acgaccgata aggatcggtt
    32641 tcgccagtcc cagcgttacc agttcctgag tggcatgcag aacgcgcgcc tcttcccctt
    32701 ccggcagaac tacgcgcttc ggcgctttgc gagcctggga gaaaatcggc ttcataaaca
    32761 ggttggtttt gtaaacgaac tcagtcagct tgtcgatgta gacgtcgaaa tcagcaatcg
    32821 gacgcgtcgc cacgcccgac tccatcgccg ctttcgcgac cgcaggagcg atcttaacga
    32881 tcaagcgcgg atcaaacggt tttggaatga tgtattccgg accaaagctc agatcctgat
    32941 cgccatacgc tgaagccacc acttcgctct gttccgcatg ggccagttct gcaatcgcgc
    33001 gtaccgccgc cagtttcatc tcttcgttga tggcggttgc gccaacgtcc agcgcgccac
    33061 ggaagatgaa cgggaagcaa aggacgttgt tgacctggtt tggatagtca gaacgaccgg
    33121 tgcagatgat ggcatccgga cgcacttctt tcgccagcgg cggcagaatt tccggttccg
    33181 gattcgccag cgccagaatc attggtgccc gagccatttt cttcaccatt tcctgggtca
    33241 gcactttcgg gccggaacag cccaggaaaa tatccgcacc ttcaatcaca tcatcgaggg
    33301 tacgtttgcc gtcatccacc accgcatatg cggctttggt ttccgccatg tttggctcac
    33361 ggccttgata gataacgcct tttgaatcgc aaaccacgat gttatgtttt tgcagaccca
    33421 gcgctaccag cagattcata caggcgattg ctgcggcacc cgcgccggaa accaccatcc
    33481 gcacgtcgga gatgtttttc tccaccacgc gcaagccgtt gaggatggcg gcagtgctga
    33541 tgattgccgt gccgtgctga tcgtcgtgga ataccggaat attcatccgc tcgcgcagtt
    33601 tctgttcaat atagaaacat tctggcgctt taatgtcttc gaggttgatt ccgccgaagg
    33661 tcggttcgag cgcagcgaca acttcaataa atttgtccgg gtcgagttca tcaacttcaa
    33721 tgtcaaatac atcaatcccg gcgaatttct taaacagaac gcccttgcct tccatcaccg
    33781 gtttgccagc cagcgcgcca atgttgccta accccagcac cgccgtaccg ttagagatca
    33841 ccgccaccag gttaccgcgg gcggtatatt tgtaggcttt taacgggtct ttttcgattt
    33901 caagacaagg tgcggcaacg ccgggtgagt aggccagcgc cagatcgcgc tgcgttgcca
    33961 gaggcttggt tggagaaacc tggattttcc ctggaactgg aaattcatgg aaatcaagtg
    34021 cactttgttt taactggtca tccatttggt tgttcctttc acgtaacgtt cacaaataaa
    34081 gtgtgtgggc aacaccccct gcccacaacg tggcgcacat tattaccttg ccggagtcta
    34141 cagactttga gcaagtccaa actctcacca ttaatataat gttttggtaa taatcctata
    34201 acactgatgt tacctgctta atccagcaat accatgcctg tctgctatgc ttttttgatg
    34261 cgtttagcga aatttctcag aagtgtgaat taacgcactc atctaacact ttacttttca
    34321 aggagtattt cctatgaacg agttagacgg catcaaacag ttcaccactg tcgtggcaga
    34381 cagcggcgat attgagtcca ttcgccatta tcatccccag gatgccacca ccaatccttc
    34441 gctgttactc aaggctgccg gattatcaca atatgagcat ttaatagacg atgctatcgc
    34501 ctggggtaaa aaaaatggca agacccagga acaacaggtg gtcgcagcgt gtgacaaact
    34561 ggcggtcaat ttcggtgctg aaatcctcaa aatcgtaccc ggtcgcgtgt caacagaagt
    34621 tgatgcacgc ctctcttttg ataaagaaaa gagtattgag aaggcgcgcc atctggtgga
    34681 cttgtatcag caacaaggcg ttgagaaatc acgcattctg atcaagctgg cttcgacctg
    34741 ggaaggaatt cgcgcggcag aagagctgga aaaagaaggt attaactgca acctgacgct
    34801 gctgttttct tttgcacagg cacgggcctg tgcggaagca ggcgtttttc tgatttcgcc
    34861 gtttgtcggg cgtatttatg actggtatca ggcacgcaag ccgatggacc cgtatgtggt
    34921 ggaagaagat ccgggcgtta aatcggtgcg caatatctac gactactata agcaacacca
    34981 ctatgaaacc attgtgatgg gcgcgagctt ccgtcgcacc gaacaaatcc tcgccttaac
    35041 cggctgcgat cgactgacta tcgcaccgaa tttgctgaag gagctgcagg aaaaagtttc
    35101 gccagtggta cgtaaattaa tcccaccttc tcagacgttc ccacgcccag ctcccatgag
    35161 cgaagcggag ttccgttggg agcacaatca gtatgcgatg gcggtagaaa aactgtctga
    35221 aggcattcgt ctgttcgccg ttgatcaacg caaactggaa gatcttcttg ccgccaaact
    35281 ataaaccagc cacggagtgt tatatgtccc gaaaagacct tgccaatgcg attcgcgcac
    35341 tcagtatgga tgcggtacaa aaagccaatt ctggtcatcc cggcgcgccg atgggcatgg
    35401 ctgatattgc cgaagtgctg tggaacgatt ttcttaaaca taaccctacc gacccaacct
    35461 ggtatgatcg cgaccgcttt attctttcca acggtcacgc gtcgatgctg ctctacagtt
    35521 tgctgcatct gaccggttac gacctgccgc tggaagagct gaagaacttt cgtcaactgc
    35581 attcgaaaac cccaggccac ccggagattg gttatacgcc aggcgttgaa accaccaccg
    35641 gtccacttgg acaaggtttg gcgaacgccg tcgggctggc gatagcagag cgtacactgg
    35701 cggcgcagtt taaccagcca gaccatgaga tcgtcgatca cttcacctat gtgtttatgg
    35761 gcgacggctg cctgatggaa ggtatttccc acgaagtctg ttcgctggca ggcacgctgg
    35821 gactgggcaa gctgattggt ttttacgatc acaacggtat ttccatcgac ggtgaaacag
    35881 aaggctggtt taccgacgat acggcaaaac gttttgaagc ctatcactgg catgtgatcc
    35941 atgaaatcga cggtcacgat ccgcaggcgg tgaaggaagc gatccttgaa gcgcaaagcg
    36001 tgaaagataa gccgtcgctg attatctgtc gtacggtgat tggctttgga tcgccgaata
    36061 aagcaggtaa ggaagaggcg cacggcgcac cgctggggga agaagaagtg gcgctggcac
    36121 ggcaaaaact gggctggcac catccgccat ttgagatccc taaagatatt tatcacgcct
    36181 gggatgcccg cgaaaaaggc gaaaaagcgc agcagaggtg gaatgagaag tttgccgcct
    36241 ataaaaaggc tcatccgcaa ctggcagaag agtttacccg tcggatgagc ggtggtttac
    36301 cgaaggactg ggagaaaacg actcagaagt atatcaatga gttgcaggcg aatccggcga
    36361 aaatcgccac ccgtaaggct tcgcaaaata cgcttaacgc ttatggcccc ttgctaccgg
    36421 agctgctcgg cggttcggcg gatctggctc ccagcaacct gaccatctgg aaaggttctg
    36481 tttcgctgaa ggaagatccg gcgggcaact acattcacta cggggtgcgt gaatttggca
    36541 tgaccgctat cgccaacggc atcgcgcacc acggcggctt tgtgccgtat accgcgacgt
    36601 tcctgatgtt tgttgaatac gcccgtaacg ccgcgcggat ggcggcactg atgaaagcgc
    36661 ggcagattat ggtttatacc cacgactcaa ttggcctggg cgaagatggt ccgacgcacc
    36721 aggctgttga gcaactggcc agcctgcgct taacgccaaa tttcagcacc tggcgaccgt
    36781 gcgatcaggt ggaagcggcg gtgggctgga agctggcggt tgagcgccac aacggaccga
    36841 cggcactgat cctctcaagg cagaatctgg cccaggtgga acgtacgccg gatcaggtta
    36901 aagagattac tcgtggcggt tatgtgctga aagacagcgg cggtaagcca gatattattc
    36961 tgattgccac cggttcagag atggaaatca ccctgcaagc tgcggagaaa ttagcaggag
    37021 aaggtcgcaa tgttcgcgtg gtttccctgc cctcgaccga tattttcgac gcccaggatg
    37081 aggaatatcg ggagtcggtg ttgccttcta acgttgcggc tcgcgtggcg gtggaagcag
    37141 gtattgccga ttactggtac aagtatgttg gtctgaaagg ggcaattgtc gggatgacgg
    37201 gttatgggga atctgctccg gcggataagc tgttcccgtt ctttggcttt accgccgaga
    37261 atattgtggc aaaagcgcat aaggtgctgg gagtgaaagg tgcctgatag tgatcgccgg
    37321 atgctgattg ccggatgcga cgctgacgcg tcttatccgg cctacagcgt cttatccggc
    37381 ctacatgtcc ccgccatttt gtttaacggg tgatccacaa cgtgggccag gcgtctggcc
    37441 catgccagtt atcgcaggtg ggttctgcgg cgtaacgcac caggcgaaaa cgctgaccgt
    37501 caaagcgcca gcgcgcctga atgccacaat cgcttaatcc gcgccctttc gctaaggtca
    37561 ccagttcacg cgatttctca tcaaatgttg cgttcatcag ttccagttca ttcgtctcct
    37621 gcccgttgtt gaacggcaaa cgcaaccgaa ccgggcgcga ggctagcggc tttttacgcg
    37681 acacaatcca tgccaaatca atggtgttat aggcccctgc ctcacagctg atcatcatca
    37741 gcgctttatc atcagtcagc gcagtgacat tcacctcacg acgtaacgga tcaagcgagc
    37801 agcgcagacc attcatccgc cagtttccat aatccagcaa atcgttgcgt tcttcgagtg
    37861 agagtggcgt cggcgttggg ttaaccaccg cgacctcttt cagcgcaggc gcgggcggta
    37921 cgctgagcgg cggttcgtcc cctttcttga tccacgcggt ttcactgcca acgcgctttt
    37981 gctgagcatc aataaacaac aacgccgctt ttaagccact cagagaaatg gtctgattgc
    38041 cgtcgcgtaa ggtgatcgcc ttcccttcct gaatcatctg caaaaacgcg gtgatggttg
    38101 ccgtatcatc ggttactaat aaccatggtg aaatccgcca cttgtcacca cttaacgcca
    38161 acggctcacc atctaacagc agccgtggcg ctatctctcc ttcagacgca tccggcgact
    38221 tcaacccgcc gcgctcaata cgtaaaacgg catcggtatg cgccccggcg ctgcggctca
    38281 gggtcatcac cagtccatta tgatcgcccg tattgcgcgc cacgcagaag ttttggttat
    38341 tacaggtgac ctgccagtcg gaaaacgccc gttgcgctag tgccgcccac accaaagacg
    38401 tcggcaacaa agcgaaaaaa aagagaagga aaatgcgata gcgcatggac ggtacgaccc
    38461 cagaagttca aacaaacagc acaagtcgta tcttcgttgc gcaggtagcg cggctcaatc
    38521 agatttgtcg gattgagcca ataataaaca acgaaatatt attgttattt ttcagtccat
    38581 taaatgtgac gtttgcaaat agttaagcaa taacaccgtc ttaccgtcac gtatctcgcc
    38641 ggttttgatc atctccagcg cctggctgaa cggcagctcg agcacttcaa tatcttcatc
    38701 ttcaacaccg ccaccggcgt tagcgcgctg attgtcactg tattcggcga taaaaaagtg
    38761 gattagctca gtcacaccgc caggcgacat atacagttca aataatttgc gcacttcgcc
    38821 cacttcatag cccgtctctt caatcgcttc tttacgaatg cacacttccg gttcgtcgtt
    38881 atccagcagc ccggcgcagg tttcaatcag ctgcccgctt tcattgccat taacccaggt
    38941 agcgacacgg aactgacgaa tcagaaccac ggtctttttc ttcgcgttgt acaggaggat
    39001 cgtcgcacca ttaccgcgat cgtaaacttc acgtttatgg cggataactt agccatcttt
    39061 gcgcgtgaga tcgtaagtaa tgttgtgcag ggtgaaatag ttatcggaga gaattttgtc
    39121 tttaatgagg gtgatttgtt gcgtcatacc gactccacag cgcgaaatga acaattatat
    39181 tacgctgtga agtcgggttt gtctgcgcag gctatcagtg agatttgact gattttaccc
    39241 ccagccagtc aataatcccc tgtgccgcat gacgtccttc tgccattgcg gtaaccacca
    39301 gatccgcacc acgcacggcg tcgccaccgg cgaagatttt cggattggtg gtctggtaac
    39361 ggtactggct ttccacatcc gcgatgatgc ggccccattt gtctaccgtt acaccgtgcg
    39421 actccagcca cggcatcccg tgtggattga agccaaacgc cataatcacc gcatcggctg
    39481 gcatgacaaa ttcactgccc tcaatcggta caagtcgccg gcgcccctgg gcatccggct
    39541 cgccaagacg cgtgcgcagg aaacgaattc cgcaaacgtg accttgctca ttcagctcaa
    39601 gcgccaccgg ctggacgtta aattcgaagt tggccccctc ttcgcgggcg ttcttcacct
    39661 ctttcttcga gcctggcatg ttagcttcat cacgacgata agcgcaggtg acgttactcg
    39721 cgccgtggcg cagcgcggta cgcacacagt ccatcgcggt gtcgccgccg cccagtacca
    39781 cgacgttgag tccggcggta ttgataaacg gctcttccgg tagctcttcg agtcccatca
    39841 cctgtttagt gttggcgatg aggaacggca gcgcgtcata aacgcccggc gcatcttcat
    39901 tgggtaaacc cgctttcatg gaacggtaag tgcctacgcc aacgaagacc gcgtcgtatt
    39961 gttccaaaag cgaatccaaa gagacatctt tacccacttc acaattgagt tcgaagtgaa
    40021 tccccatcgc gctgaagatt tcccgacggc gtgccagcag ggatttatcc agtttgaaag
    40081 aaggaatgcc gaaagtgagc aagccaccaa tttctggatg gcgatcgtac accgtcaccg
    40141 ccacgccatt gcgggtcaga acatccgcac aggccagccc tgccggaccc gcaccgataa
    40201 tcgccacccg cttgtccact ttggtgacat ggcttaagtc aggacgccaa cctttcgcca
    40261 acgcctgatc tgaaatgtag cgttcaatgt tgccgatagt taccgcgccg tgctcatcgc
    40321 gaatagtaca ggcaccttca cacaaacggt cctgcggaca aacgcgtccg gtaatttccg
    40381 gcagggtgtt ggtcaggtga gaaagctcga cggcggcgtc gatgtttccg gctttcacca
    40441 gttcaatcca ctgcggaata tggttatgca gcgggcaggt ccattcacaa acgctgtgct
    40501 cgccgcattt aaggcagcgc gaggcttccc gttgtgcctg atcggcacgg aatggcagat
    40561 aaatttcatc aaaaccggtt ttgcgcgctt caatcgccag tttatccggc tcgccacgcg
    40621 cgggcgttgc ctgcatttgt tcgactttac tcattaccgg catttcttgt gcagcggtac
    40681 tggcatgcca cggttgatgc tcctgacgtg cggtgcgcaa gcggcgggat ttcgccatgc
    40741 cggagagtgc gacgtcagtg accagttgca gcgcgtccgc cgggcaattc tcgacacagg
    40801 caggaccgtt ttcgcgccct gcacaaaggt cgcatttatg cgccgtggct tttacttttc
    40861 ctgccgcgac gggtgtcagg acgatttgca tcgtaccaaa cggacaggcc accacgcagg
    40921 atttacagcc aatacacttt tgctgattga cctgaatgct gtcatcaacg tggctgattg
    40981 cgccattagg gcagctacgg gcgcagggcg catcttcaca atggtgacag gtcactgcac
    41041 tacgttgctg ctgatgtttg ataaccgtaa ttcggggatg aaaatggtgt tggctcagga
    41101 catgttgctc atcattgtga gccatgacac aggcgatttc acaagcatga caacccagac
    41161 attgctgact gttggccata ataaaacgat tcataacgac cttctttttt ggttgtaaaa
    41221 accttattct ttatatgagt gttgttatta cccgacttac aggggaatcg gcaatgttca
    41281 tgtgcccaga ataagtaact atttcgctat aaactgtggc agatcaaata atcccatcac
    41341 tgactaaatt gcgttttagc gaactggaac attaatgatt ttttgtggag aagacgcgtg
    41401 tgattgttaa acgacccgtc tcggccagtc tggcccgggc ctttttttac attgtgctgc
    41461 tgtcgattct ttccacgggt atcgctctgc taactctggc gagcagtttg cgcgacgctg
    41521 aggctatcaa tattgccgga tcgctgcgta tgcagagtta ccgcctgggc tacgacttgc
    41581 aaagtggcag tccacaactc aatgcacatc gccagttgtt tcagcaggca ctgcattcat
    41641 cggtattaac caatctcaac gtctggtatg tgccagaagc agtaaaaact cgctatgcgc
    41701 atctgaatgc caactggctg gagatgaata atcggctcag caagggcgat ttgccgtggt
    41761 atcaggccaa tattaataat tatgttaatc agatagacct gttcgtgctg gctttacagc
    41821 actacgctga acgcaaaatg ctgctggtgg tggcgatttc cctggctggc ggtatcggta
    41881 ttttcacgct ggtctttttt actttgcgcc gtatacgcca tcaggtggtt gccccgctga
    41941 atcagctggt taccgccagt caacgtattg aacacgggca gttcgactcg ccgccgctgg
    42001 ataccagtct gccgaatgag cttggtctgc ttgcaaaaac ctttaaccag atgtcgagcg
    42061 agctgcataa attgtacctt tcgctggagg cgtcagtaga agaaaagacc cgcgatctcc
    42121 acgaggccaa gcgtcgtctg gaggtgttgt atcaatgttc gcaggcgctg aacacaagcc
    42181 agattgatgt gcattgtttc cgccatattt tgcagattgt tcgcgacaat gaagcggctg
    42241 aatatctgga gttaaatgtc ggtgaaaact ggcggattag cgaagggcaa ccaaacccgg
    42301 aattgccgat gcagatttta ccggtgacaa tgcaagagac ggtttacggc gaactgcact
    42361 ggcaaaatag tcacgtttca tcatcagaac cgctgcttaa cagcgtttcg tcgatgctgg
    42421 gacgcggttt gtactttaat caggcgcaaa agcattttca gcagttattg ttgatggaag
    42481 aacgtgcaac catcgcccgc gaattgcacg actcgctggc tcaggtactt tcttacttac
    42541 gtatccagtt gacgttactg aagcgttcga taccggaaga taacgccacc gcacaaagta
    42601 tcatggccga tttttcccag gcgttgaatg atgcttatcg gcagttacgc gagctgttga
    42661 ctactttccg cctgacgctg cagcaggcgg atctcccctc cgcgttgagg gaaatgctgg
    42721 atacgttaca aaatcaaacc agcgccaaac tgaccctcga ctgccgtctg ccaaccctgg
    42781 cgctggatgc gcaaatgcag gtgcatttgt tgcaaattat tcgcgaagcg gtgctgaatg
    42841 cgatgaagca cgccaacgcc agcgaaatcg ccgtcagttg cgtcaccgcg ccggacggca
    42901 atcacacggt ttatatccgt gataacggga ttggtatcgg tgaaccgaaa gaacccgaag
    42961 gtcattatgg tctgaatatc atgcgcgaac gcgcggaacg gctgggtggg acgctgactt
    43021 tttcacaacc ttccggcggc ggcacgttag tgagtattag ctttcgctct gcggagggtg
    43081 aggaaagtca gttgatgtaa tgcctcctac tgaccaaaga atacttgcac ttaaggttca
    43141 gtataaaagg gcatgataat ttacattaac tccttttttt ctccacgatt ggctcgtacc
    43201 ttgccgctac agtgaagcaa gtcaagccta caacgatacg cagaaacacg aggtcctctt
    43261 ttaatggcga atttctttat tgatcgcccc atttttgcct gggtgctggc aatcctgttg
    43321 tgtctgacag ggaccctggc gattttttca ttgcccgttg aacaataccc cgatctcgcg
    43381 ccaccgaatg tgcgagtgac cgctaactat cccggcgcat cggcccagac gctggaaaac
    43441 accgtgaccc aggttatcga gcaaaatatg accggcctcg ataatctcat gtatatgtca
    43501 tctcagagca gtggcatcgg tcaggcatct gtcactttaa gttttaaagc aggcaccgat
    43561 ccggacgaag ccgtgcagca agtacaaaac cagctgcaat cagccatgcg aaagttaccg
    43621 caggcggtgc aaaatcaggg cgtgacggtg cgtaaaaccg gcgataccaa tattctgacc
    43681 attgctttcg tctctaccga tggttcgatg gataaacagg atattgccga ttatgttgcc
    43741 agtaatattc aggacccgtt aagccgcgtg aatggcgtcg gggatatcga tgcctatggt
    43801 tcgcaatatt ccatgcgtat ctggctggac ccggcgaaac tcaacagttt ccagatgacg
    43861 gctaaagatg tcactgatgc cattgagtca cagaacgcgc agattgcggt tgggcaactt
    43921 ggtggtacac cttccgtcga taagcaggcg ctcaacgcca ccattaacgc ccagtcactg
    43981 ctgcaaacac cagaacagtt ccgcgatatc accttgcggg tgaatcaaga cggctcagag
    44041 gtaaggctgg gcgatgtcgc caccgtcgaa atgggggcgg agaaatacga ttatcttagc
    44101 cgcttcaatg gtaagccagc ctccgggctg ggggtaaaac tggcctccgg cgcaaacgaa
    44161 atggcgacag cggagctggt gctcaatcgt ctcgacgagc tggcgcagta cttcccgcac
    44221 ggactggaat acaaggtggc gtatgaaacc acctcgtttg ttaaagcctc cattgaagac
    44281 gtggtgaaaa cgctgctgga agctatcgcc ctggttttcc tcgttatgta tctgttcctg
    44341 caaaacttcc gcgccacgct gatacccact atcgccgtgc cggtggtgtt gatgggaacc
    44401 ttctccgtac tttacgcctt cggttacagc gtcaacacct taaccatgtt cgcgatggta
    44461 ctggcgatcg gtctgctggt ggatgacgca atcgtggtgg tggaaaacgt cgaacgtatt
    44521 atgagtgagg aaggactcac tcctcgcgaa gccacacgta aatcgatggg gcagatccag
    44581 ggggcactgg tcgggattgc gatggtgctt tcggcggtat ttgtaccaat ggccttcttc
    44641 ggcggcacca ccggtgccat ctatcgccag ttctctatta ccattgttgc ggcgatggtg
    44701 ctgtcagtac tggtagcgat gatcctcact ccggctctgt gtgccacact acttaagcca
    44761 ctgaaaaaag gtgagcatca cgggcaaaaa ggcttttttg cctggtttaa ccagatgttt
    44821 aaccgcaacg ccgaacgcta cgaaaaaggg gtggcgaaaa ttctccaccg cagcctgcgc
    44881 tggattgtga tttatgtcct gctgcttggc ggcatggtgt tcctgttcct gcgtttgccg
    44941 acgtcgttct taccgctgga agaccgtggc atgtttacta cctcggtaca attgcccagc
    45001 ggttcaaccc aacaacagac cctgaaagtc gttgagcaaa tcgagaaata ttacttcacc
    45061 catgaaaaag acaacatcat gtcggtgttt gccaccgttg gttctggccc tgggggtaac
    45121 gggcaaaacg tggcgcgaat gtttatccgc ctgaaagact ggagcgaacg cgacagtaag
    45181 accggcacct cgtttgccat tatcgagcgt gcaacaaagg cgtttaacaa gattaaagaa
    45241 gctcgcgtta tcgccagcag cccgccagca attagcggtc ttggtagttc tgcgggtttt
    45301 gatatggagt tgcaggacca cgctggagca ggtcacgatg cgctgatggc agcacgtaac
    45361 cagttgctgg cgctggcggc ggaaaacccg gagctaaccc gtgtgcgcca taacggcctc
    45421 gacgacagtc cgcagttgca gattgatatc gaccagcgta aagctcaggc gctgggcgtt
    45481 gctatcgacg atattaacga cacactgcaa accgcctggg gttcgagcta tgtgaatgac
    45541 tttatggatc gcggtcgcgt gaagaaagtc tatgtgcagg cagctgcgcc gtatcgcatg
    45601 ctgccagatg acatcaatct ctggtatgtc cgaaataaag atggcggcat ggtgcccttc
    45661 tctgctttcg cgacctcacg ctgggaaaca ggctcgccgc gtctggaacg ctataacggt
    45721 tattctgcgg ttgagattgt tggggaagcc gcaccggggg tcagtaccgg tacggcgatg
    45781 gatattatgg aatcgttagt gaagcagctg ccaaacggct ttggtctgga gtggacggcg
    45841 atgtcgtatc aggagcgact ttccggcgcg caggctccgg cgctgtacgc catttccttg
    45901 ctggtggtat tcctgtgtct ggctgcgttg tatgaaagct ggtcggtgcc gttctcggta
    45961 atgctggtcg tgccgctggg ggtaatcggc gcgctgctgg caacctggat gcgcgggctg
    46021 gaaaacgacg tttacttcca ggtgggcctg ttaacggtca ttggtttatc ggcgaaaaac
    46081 gccatcctga tcgtcgagtt tgctaacgag atgaaccaaa aaggccacga cctgtttgaa
    46141 gcgacgctcc acgcctgccg tcagcgttta cgcccgattc tgatgacctc gctggcattt
    46201 atcttcggcg tattgccaat ggcaaccagc acgggtgccg gttccggtgg tcagcatgcg
    46261 gtgggtactg gcgtaatggg cgggatgatt tcagccacta ttctggctat ttacttcgtg
    46321 ccgctgttct ttgtgctggt gcgccgccgc ttcccgctaa agccgcgccc ggaataagca
    46381 ataaaaaagg cgacatgcca atgtgtcgcc tttttcaact ttccgattaa gaacctgctc
    46441 agcgggttct tgctgtttat actttgtctc aggaattact tacgaagcat aacttcgata
    46501 aagtctttcc agttccccag ttcacgttca atcataacaa cctctcttat aattatgggt
    46561 attctacgga aacaatatac cgtggtgaag ctaatttact cgattgctgc gatgactacc
    46621 tccgggggga caaatcttat gtaaatacta tggtcctgcg gtgatgattt gtatgtgata
    46681 cacagcaaca tttcgagata ttcatacggc atctaatact gatttaattc aggttaaaat
    46741 acagacagat aacaagatga atattctta
//
LOCUS       DAZPEP010000013        46232 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZPEP010000013 DAZPEP010000000
VERSION     DAZPEP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46232
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.013"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUT9_000757"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="ACHUT9_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1347311.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="ACHUT9_000758"
     CDS             complement(280..873)
                     /locus_tag="ACHUT9_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1347312.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUT9_000759"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="ACHUT9_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1347313.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="ACHUT9_000760"
     CDS             1788..2183
                     /locus_tag="ACHUT9_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1347314.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUT9_000761"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="ACHUT9_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFR1347315.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUT9_000762"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="ACHUT9_000762"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFR1347316.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUT9_000763"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="ACHUT9_000763"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFR1347317.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUT9_000764"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="ACHUT9_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFR1347318.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUT9_000765"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="ACHUT9_000765"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFR1347319.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUT9_000766"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="ACHUT9_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFR1347320.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="ACHUT9_000767"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="ACHUT9_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFR1347321.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="ACHUT9_000768"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="ACHUT9_000768"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFR1347322.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="ACHUT9_000769"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="ACHUT9_000769"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFR1347323.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUT9_000770"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="ACHUT9_000770"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFR1347324.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGISIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUT9_000771"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="ACHUT9_000771"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFR1347325.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUT9_000772"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="ACHUT9_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFR1347326.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUT9_000773"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="ACHUT9_000773"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFR1347327.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUT9_000774"
     CDS             17158..17709
                     /gene="yjgA"
                     /locus_tag="ACHUT9_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFR1347328.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUT9_000775"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="ACHUT9_000775"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFR1347329.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUT9_000776"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="ACHUT9_000776"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFR1347330.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUT9_000777"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="ACHUT9_000777"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFR1347331.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUT9_000778"
     CDS             complement(21354..22376)
                     /gene="ytfT"
                     /locus_tag="ACHUT9_000778"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFR1347332.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUT9_000779"
     CDS             complement(22390..23892)
                     /gene="ytfR"
                     /locus_tag="ACHUT9_000779"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFR1347333.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUT9_000780"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="ACHUT9_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFR1347334.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUT9_000781"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="ACHUT9_000781"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFR1347335.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="ACHUT9_000782"
     CDS             complement(26207..26548)
                     /locus_tag="ACHUT9_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFR1347336.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUT9_000783"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="ACHUT9_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFR1347337.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUT9_000784"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="ACHUT9_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFR1347338.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUT9_000785"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="ACHUT9_000785"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFR1347339.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="ACHUT9_000786"
     CDS             33227..34570
                     /locus_tag="ACHUT9_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFR1347340.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34650..34856)
                     /locus_tag="ACHUT9_000787"
     CDS             complement(34650..34856)
                     /locus_tag="ACHUT9_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFR1347341.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35181..35735
                     /locus_tag="ACHUT9_000788"
     CDS             35181..35735
                     /locus_tag="ACHUT9_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFR1347342.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35825..36736)
                     /gene="ytfI"
                     /locus_tag="ACHUT9_000789"
     CDS             complement(35825..36736)
                     /gene="ytfI"
                     /locus_tag="ACHUT9_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtfI"
                     /protein_id="HFR1347343.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPV"
     gene            complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="ACHUT9_000790"
     CDS             complement(36948..37688)
                     /gene="cysQ"
                     /locus_tag="ACHUT9_000790"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFR1347344.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37878..39821
                     /gene="cpdB"
                     /locus_tag="ACHUT9_000791"
     CDS             37878..39821
                     /gene="cpdB"
                     /locus_tag="ACHUT9_000791"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFR1347345.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSNKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39939..40319)
                     /locus_tag="ACHUT9_000792"
     CDS             complement(39939..40319)
                     /locus_tag="ACHUT9_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFR1347346.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40408..41268
                     /gene="qorB"
                     /locus_tag="ACHUT9_000793"
     CDS             40408..41268
                     /gene="qorB"
                     /locus_tag="ACHUT9_000793"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1347347.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41376..42341
                     /locus_tag="ACHUT9_000794"
     CDS             41376..42341
                     /locus_tag="ACHUT9_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1347348.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42449..43111
                     /gene="ytfE"
                     /locus_tag="ACHUT9_000795"
     CDS             42449..43111
                     /gene="ytfE"
                     /locus_tag="ACHUT9_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFR1347349.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="ACHUT9_000796"
                     /gene_synonym="dagA"
     CDS             complement(43156..44568)
                     /gene="cycA"
                     /locus_tag="ACHUT9_000796"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFR1347350.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44800..45495)
                     /locus_tag="ACHUT9_000797"
     CDS             complement(44800..45495)
                     /locus_tag="ACHUT9_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein mom"
                     /protein_id="HFR1347351.1"
                     /translation="MGKEKKSRILTKPCVIEYEGRIVGYGSRELRVETISCWLARTII
                     QTKHYSRRFVNNSWLHLGVFSGRDLVGVLQWGYALNPNSGRRVVLETDNRGYMELNRM
                     WLHDDMPRNSESRAISYALKTIRLLYPSVEWVQSFADERCGRAGVVYQASNFDFIGSH
                     ESTFYELDGEWYHEITMNAINRGGKRGEYLRANKERAVVHKFNQYRYIRFLNKRARKR
                     LNTKLFRIQPYPK"
     gene            complement(45446..45631)
                     /locus_tag="ACHUT9_000798"
     CDS             complement(45446..45631)
                     /locus_tag="ACHUT9_000798"
                     /inference="COORDINATES: protein motif:HMM:NF021626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1347352.1"
                     /translation="MDSVRCKNCNKLLFKGGFKHIEIKCPRCKRYIVISSAREHPTEL
                     YCGKREEITHSDKTLRY"
     gene            complement(45756..>46232)
                     /locus_tag="ACHUT9_000799"
     CDS             complement(45756..>46232)
                     /locus_tag="ACHUT9_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000050443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1347353.1"
                     /translation="PYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREA
                     RRQEIEAWRAEQEAKPFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTNMTWSDAD
                     NQQVQLTTQELEELATAMIQAIVERNDEIYRCQREMKEQLSLLPTLDEVRAFRPGD"
BASE COUNT        11312 a        12316 c        11470 g        11134 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc ccctgcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtctag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgctg
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaagaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc tccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccaaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gaccccagaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cgactttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ttactgcata gcactttggt taagccatct ctgtttgcag acgcataacc
    34681 tgacggttaa cttcggacat cactgacaaa tgcagcttat ccttcacttt tgggataaga
    34741 atcttaccct tatcaaattc aaaagcgcca acgtccttga tgtataaccg tccacggaac
    34801 aggatcttta cgtacttcgc cacctgaagt gggttgtacc gttggaaaat tttcattgtt
    34861 atctcctgct gagtattacg cctgtgcggt gccacaatcg gcccaactat tatgaggcgc
    34921 aaattttaat gcttagtgac tatagactat ccgggcaatg tttccaccgc gtataacttt
    34981 ttttacctaa aggttacaat tattcagaat tatcttttta ccgaagcgcg gtcttcagta
    35041 taagcattca tttttcatat gatttgtgcg cttgaccgca aactggcatc acacttgcgg
    35101 gaaattcgat aaatagcaca tatgattaaa actcagaccc aagtggtcgg atcacctgca
    35161 tatcataaga aggaaacacc atgaccctac gcaagattct ggcactcacc tgcctgctgt
    35221 tgccgatgat ggcttccgca catcagttcg aaaccggtca gcgagtgccg ccgattggca
    35281 ttaccgatcg gggcgagttg gtgcttgata aagatcagtt tagctacaaa acctggaaca
    35341 gcgcgcagtt agtgggaaaa gtgcgagtac tgcaacatat tgctggtcgc acctctgcaa
    35401 aagagaaaaa cgcgacgctg attgaagcga ttaaatcagc gaagttaccg cacgatcgtt
    35461 accagaccac cactattgtt aacaccgacg acgcgattcc gggttcaggg atgtttgtgc
    35521 gcagcagtct ggagagtaat aaaaagcttt atccctggtc gcagtttatt gtcgatagca
    35581 atggcgtcgc gcgcggtgcc tggcagctgg atgaagaaag ttccgctgta gtggtgctgg
    35641 ataaggatgg tcgcgtgcaa tgggccaaag acggggcgct cactcaggaa gaggtgcagc
    35701 aagtgatgga cctgctgcaa aagttgctca aataatggca acaacataga ttctgcaaaa
    35761 ataattttcc cctatcgtta tctatgggat aggggaatta agtctctgag ttcttacgcc
    35821 gaactcatac tggtaacgct agttgctctt ttttaaacgc ctcaatggtc gacttatatt
    35881 tctcgcaaat cttttctttg gtaatattac tatctgcggc cagattcgca cttactatcg
    35941 catccacttg ttcggctaat tccacttgtt ggcaacctgc attaaccaga aaattataaa
    36001 taagggagta atcttcatcc tccaaaacaa cagaggaacc attacgaaaa cgttcatccc
    36061 ctccatagat tttaagcgca aagcccaaag attctatgac tatggcaaaa ttatcatcaa
    36121 ttattttttc gtgaatatac catccatcgc attctttaat tccacctcct ggtatttgga
    36181 gttcaaattt accccgtttt aatttgctta ctatatcctc caataaggca taacctaaag
    36241 acacgtcacc aatcgcctgg tgaatataac ctttaatcct tttcggtatg tcagactgtt
    36301 ttttctgcag aatatcacag gcatacaatc ttctgttttg ttctaacgga aactcgcggt
    36361 ttttgaaatc catgaatgag ttatactcat ttattgttat gggaatttct tcgtattcaa
    36421 atccactacc tcgcaatgtc attgtatatt cggcaccgtt atctttaagg ctgaaactga
    36481 taaggaactt atccatggcg ttaacagttc cattttttga tgaaagtacc tgataactat
    36541 ttttatcaat ctgagttgcg caacgaaaga actccctcaa agttaacagg ctatcataat
    36601 tcgaaatcca atcttttttc ttaaatgcat gctggatatt gttaaaaaaa ttgtttgaat
    36661 gtgtttttgt atacgatgac tttacaaata actccaccgc accaataaaa ttattgtgtc
    36721 tgatcctggg taacatatga caaccttatt ttatttggtt attttatgtc gccattaaat
    36781 atctcattag agaagtaaac tatcaaaaaa cgctcattat taaaatgtaa cttattagca
    36841 attgaatttt tgcactttaa atacaatctc tttttattta taacttttta ggttgggaga
    36901 aatcaggatg gcagaatcag gaaatacact gtttctgcca tctgaattta gtaaatagac
    36961 actctgaacc ccggattcag gaacgactcg cgcggagtgt aatccagcgg tttaccctgc
    37021 cagtcgtgaa cgtgcgctcc ggcagctgca gccacagcat gtccagcggc ggtgtcccaa
    37081 atattcgttg gtccgaagcg cgggtacagc tgcgcctgtc cttccgccac caggcagaat
    37141 ttcagcgaag agccgatgga cgtggtctga tgttcgccaa gctgttgcag atactctttc
    37201 agctccgcat ctgcatggga acggctgata accaccagcg gcgggcgcgc atcgcggacc
    37261 tgaatctgct tgcgcacacc gcactcttct ttccaggcct tgccttctgc cgcgctgtac
    37321 attacgttca ttaccggcgc atacaccaca cctaaaatcg gtttgccatg gtcaatgagc
    37381 gcaatgttaa cggtgaattc gccattacgt ttaataaact ctttagtacc atccagcggg
    37441 tctaccagcc agtaacgctg ccagtgctga cggacttccc aaccgggagg atcttcttca
    37501 gaaaggaccg gaatatccgg tgtcagcgta cgtaaaccgt ccatgataac ggtgtgtgcg
    37561 gcaatatccg ctgccgttac cggagaattg tccgctttgc tgacgacgtc catcggtttc
    37621 gtcccgtcgt agacctgcat aatggcatcg cctgcattcc gtgcaagctg gcatacttga
    37681 tctaacattt ctccacctcg tctctgtgag cggtgttaac ttattgtttt acttataccc
    37741 tatcgttaat gaatgcgcca actgtgatag tgtcatcatt ttcaaagcgt aaaattgtgg
    37801 cattcttcac tgttctataa gtaagacgtt tattctccct tttctttcgt attcccgatg
    37861 ataaaaggat gtccctgatg attaagttta gcgcaacgct cctggccacg ctgattgccg
    37921 ccagtgtgaa tgcagcgacg gtcgatctgc gtatcatgga aaccactgat ctgcatagca
    37981 acatgatgga tttcgattat tacaaagaca ccgccacgga aaaattcgga ctggtacgta
    38041 cggcaagcct gattaacgat gcccgcaatg aagtgaaaaa cagcgtactg gtcgataacg
    38101 gcgatttgat tcaggggagt ccgctggcag attacatgtc ggcgaaagga ttaaaaacag
    38161 gtgatgttca tccggtttat aaggcgctga atacgctgga ttatacggtc ggtacactcg
    38221 gcaatcatga atttaactac ggtctggatt acctgaaaaa tgcgctggcg ggagcgaaat
    38281 tcccttatgt aaatgccaac gtcattgacg ccagaaccaa acagccaatg tttacaccgt
    38341 atttaattaa agataccgaa gtggtcgata aagacggaaa aaaacagacg ctgaagattg
    38401 gctatattgg cgtcgtgccg ccgcaaatca tgggctggga taaagctaat ttatccggga
    38461 aagtgacggt gaatgatatt accgaaaccg tgcgcaaata cgtgcctgaa atgcgcgaga
    38521 aaggtgccga tgtcgttgtc gttctggcac attccgggct gtctgccgat ccgtataaag
    38581 tgatggcgga aaactcagtt tattacctca gtgaaattcc gggcgttaac gccattatgt
    38641 ttggtcatgc tcacgccgtt ttcccgggta aagattttgc tgatatcgaa ggggctgata
    38701 tcaccaaagg cacgctgaat ggtgttccgg cggtaatgcc gggcatgtgg ggcgatcatc
    38761 ttggggtggt cgacttacaa ctcagtaatg acagcggtaa atggcaggtg acgcaggcga
    38821 aagcggaagc tcggccgatt tacgacatcg ccaataaaaa atccctcgcg gcggaagaca
    38881 gcaagctggt agaaacactc aaagccgatc acgatgccac acgccagttc gtcagcaagc
    38941 caatcggtaa atctgccgac aatatgtata gctatctggc gctggtgcag gacgatccga
    39001 ccgtgcaagt cgtgaacaac gcgcaaaaag cgtatgtcga acattacatt cagggcgatc
    39061 cggatctggc aaaactgccg gtgctttcag ctgccgcacc gtttaaagtt ggtggtcgca
    39121 aaaatgaccc ggcaagctat gtggaggtgg aaaaaggcca gttgaccttc cgtaatgccg
    39181 ccgatcttta tctctacccc aatacgctga ttgtagtgaa agccagcggt aaagaggtga
    39241 aagagtggct ggagtgctcc gccggacagt ttaaccagat tgatcctaac agcacgaaac
    39301 cacagtcact catcaactgg gatggtttcc gcacttataa ctttgatgtt attgatggtg
    39361 tgaattatca gattgatgtt acccagcccg cccgttatga cggcgagtgc cagatgatta
    39421 ataccaatgc ggaaaggatt aagaacctga cctttaatgg caagccgatt gatccgaacg
    39481 ccatgttcct cgttgccacc aataactatc gcgcttacgg cggcaaattt gccggtacgg
    39541 gcgacagcca tatcgctttt gcttcaccgg atgagaaccg ctcggtgctg gcagcgtgga
    39601 ttgctgatga gtcgaaacgt gcgggggaaa ttcacccggc ggcagataac aactggcgtt
    39661 tagcaccgat agctgccgat aagaaactgg atatccgttt cgaaacttcc ccgtcaaata
    39721 aagccgcagc gtttattaaa gagaaagggc agtatccgat gaataaagtc gcgaccgatg
    39781 atatcgggtt tgcgatttat caggtggatt tgagtaagta aaacacttct ttttcggcct
    39841 ataaatcatc aatcgcatcc ggcatttatt ggcggatgcg atgctggcgc atcttatccg
    39901 ccctacaagt catacaccgt aggccgcgca tccggcagtt atgccgcacg ttcatcccgc
    39961 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    40021 ctcacctgct cgcccagcgg cgtgaggctg tattcaacat gcggtggcac caccggatac
    40081 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    40141 tcgctcaccc cgccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    40201 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    40261 cactgttccg caaagagatt accctctttc agttgttgcg acaggctaac ctgactcatt
    40321 tcatacttac ctttttgtac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    40381 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    40441 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    40501 gttcgtaatc cggcaaaagc ccaggctcta gcagcacaag gcattaccgt gcgtcaggct
    40561 gactacggcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    40621 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    40681 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    40741 ctcggcctcg ccgatgagca cattgagacg gagaaaatgc tggctgattc tggcatcgtt
    40801 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    40861 ctggaacacg gcgtatttat cggtgcggcg ggcgatggca aaatcgcctc agcaacgcgg
    40921 gcagattatg cggcagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    40981 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    41041 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    41101 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    41161 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ccgcccaacg
    41221 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    41281 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    41341 tgaactttct tctgacagac caatggatgc cagtaatgat tagcggcgtg ctgtacgccc
    41401 tgttagcagg gttgatgtgg gggcttattt ttgtcgggcc gttgatcgta ccggaatacc
    41461 cggcgatgtt gcagtcgatg gggcgttatc tggcgttagg gttaattgcg ctgcccattg
    41521 cctggctggg acgcgtgcgt ctgcgtcagt tggcgcgtcg cgactggctt accgccttga
    41581 tgctcaccat gatgggcaac cttatctatt acttctgcct tgccagtgcc attcaacgta
    41641 ctggcgcgcc tgtttccacg atgattatcg gcaccctgcc ggtggtgatt cccgtttttg
    41701 ccaatctgct ttatagccag cgcgacggca aactcgcatg gggaaaactc gccccggcac
    41761 tggtttgtat tggcatcggc ctggcgtgtg tgaatattgc tgagttaaac cacggactcc
    41821 ccgattttga ctgggcacgt tatacctctg gcatcgtgct ggcgttagtt tccgtggtct
    41881 gctgggcgtg gtatgccctg cgcaacgccc gctggctgcg ggaaaatccc gacaaacatc
    41941 cgatgatgtg ggcaacggcg caggcgctgg tcacactgcc ggtttctctc atcggctatc
    42001 tcgtcgcctg ttactggctg aatatacaaa cgccggactt ctccttacct tttggccccc
    42061 gtccgctggt gtttattagt ctgatggttg cgatagccgt gctttgctca tgggttggcg
    42121 cactctgctg gaacgtcgcc agccagcgat taccgacagt gattctcggg ccgctgattg
    42181 ttttcgaaac gctggcaggt ttgctgtaca cctttttact ccgccagcaa atgccgccgc
    42241 taatgacgct gagcggtatc gcgctgttag tgattggcgt ggtcattgcg gtcagagcaa
    42301 aaccagaaaa acctttaact gaatctgtct cagaaagttg acacgctggc agtgagttaa
    42361 ataagcctct gctacgtaag ggttatagct tttgccttaa agatgcattt aaaatacatc
    42421 ttatattatt aagaatgagg tatcagctat ggcttatcgc gaccaacctt taggtgaact
    42481 ggcgctctct attcctcgcg cttcagctct gtttcgtaaa tatgatatgg attactgctg
    42541 tggcggtaag caaacgctgg cgcgcgcggc ggcacgtaaa gaactggatg ttgaggtcat
    42601 tgaagctgaa ctggcaaagc ttgctgaaca accgattgag aaagactggc gtagcgcccc
    42661 gctggcagaa atcattgacc atatcatcgt gcgctaccac gatcgtcacc gcgagcaact
    42721 gccggagctg atcctgcaag cgactaaagt cgagcgcgtt cacgccgaca aaccgagcgt
    42781 gccaaaaggg ctgacaaaat acctgactat gctgcatgaa gagctatcca gccacatgat
    42841 gaaagaagag cagatcctct tcccgatgat caaacaaggc atgggcagcc aggcaatggg
    42901 accaatcagc gtgatggaaa gcgagcacga tgaagcgggc gaactgctgg aagtgattaa
    42961 acacaccacc aataacgtca caccgccgcc ggaagcctgc accacctgga aagcgatgta
    43021 taacggtatt aatgaactga ttgatgacct gatggatcac atcagcctgg aaaacaatgt
    43081 actgttccca cgcgcgctgg cgggtgagta atctaaaagc tggatggcat tgcgccatcc
    43141 agcatgataa tgcggttatt tccgcagttc agcagcccgc ttcttaccaa taaacaacca
    43201 gcccagcccc agtgcgataa accacagcgg ggtgaccaac agcgcctggc gagtgtcatc
    43261 ttccagtgtc agcaacacga ccacgaacac aaagaacgcc atacataccc agcacatcag
    43321 cttgccgagc ggcatcttgt agatcgactt ctcatgcaga tgaggacgct gtttgcggta
    43381 aaccaggtac gagcaaagga taattgtcca gacgaacata aacagaatcg cggaaacggt
    43441 tgtaatcatc gtgaacgcgc caatcacgct aggattcaca tacaacatca ccacgccgcc
    43501 gagcagacag atacacgaga aggtcaaccc tttcgcgggt actgcgcgct tagaaagttt
    43561 agcgaacgct ttcggtgcca caccttcctg cgccagacca aacagcatac ggctggtaga
    43621 gaagacgccg ctgttagcgg aagacgccgc agaggtcagc accacaaagt tgatcacgct
    43681 ggcagccgca ggcaaaccta ccaacacaaa cagttcaaca aacgggctct tctccgggac
    43741 taccgaactc cacggcgtca cggacataat cacaatcagc gcgaagacgt agaacataat
    43801 gatacggatc ggaatggagt taatcgcgcg tggcagtgat ttctccggat ctttggtttc
    43861 cgcagcggtt gtacctacca gctcaatccc tacgaaagcg aaaaccgcta tctggaatcc
    43921 ggcaaagaag ccacttaaac ctttcgggaa ccagccgcca tcattccaca aatgcgcgaa
    43981 tgacgcttcc acaccagtcg gtgactgaaa gtgcatcgcc accataacca ggccgacgac
    44041 aatcagcgac acaatggcga cgattttgat catcgcaaac cagaactcca tctcaccgaa
    44101 cattttcacg gtggcgagat tgagcgtcag cagcagcact atcaccgcca gcgaggcgac
    44161 ccagtcggag agatcgggga accagaactg agcataagca gtgatcgcca ccacgtctgc
    44221 cataccggtt acaacccagc agaaccagta agtccagccg gtgaaatatc ctgcccacgg
    44281 cccgagtaaa tcggaagcga agtcactaaa agatttgtat tccagattcg aaagcagcaa
    44341 ttcccccatt gcccgcatca cgaaaaagag cataaaaccg atgatcatat aaacgaaaat
    44401 gatcgacggc ccggcaaggc taatcgtttt gccggacccc ataaacaacc ccgtaccaat
    44461 ggcaccgcca atggcaataa gctgaatatg tcggtttgtg agattgcgcc gtagcgactg
    44521 ttcagccgga gcctgatcat cggcaacgac ttttacctga tctaccatgt ttttttcttc
    44581 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    44641 ctgtatcggg actcatcgat attaggtaac aattaaagcg cgcaaaatta ttcaagatag
    44701 atatggaatt ctttcaagat aagccagaag cgcgcatatg gaagttattc aagataaaac
    44761 agcggcatta accgctgctt ttttattcat taagtgggat cacttcgggt aaggctgaat
    44821 cctgaatagc ttagtattta gtcgctttct tgcgcgcttg ttcaggaacc tgatgtaacg
    44881 atattgatta aatttgtgta ccacggcccg ctccttattc gctcgtaaat attcgccgcg
    44941 cttccctcca cgattaatcg cattcatcgt tatctcgtga taccactcac cgtccagttc
    45001 gtagaatgtg ctttcatggc tgccaataaa atcaaaattt gacgcctgat acacaacacc
    45061 tgcacgcccg caacgttcgt ctgcaaagga ctgaacccac tccactgacg gatacagtaa
    45121 cctgatggtt ttcagcgcgt agctgatggc ccgtgattca gagttgcggg gcatgtcgtc
    45181 gtgtagccac atgcggttca gttccatata gccccggtta tccgtttcaa gcacaacgcg
    45241 acgccctgag ttggggttaa gggcatatcc ccactgaaga acaccaacca gatcacgtcc
    45301 actgaatacg cccagatgca accaggagtt attcacaaaa cggcgggaat aatgctttgt
    45361 ctgaatgatt gtgcgggcca gccagcagga tatggtttca acgcgcagct cccttgaacc
    45421 atagccgaca atccggcctt catactcaat aacgcagggt tttgtcagaa tgcgtgattt
    45481 cttctctttt cccacaatac agctccgtgg gatgctcccg ggcactcgat atgacaatgt
    45541 aacgtttaca gcgaggacat ttgatttcta tatgcttaaa acctccttta aacagcaatt
    45601 tattgcaatt tttacaacga acagagtcca taatttcccc cagtgtaata acaggatata
    45661 gctgcccgga gggctggggg tatttaatca cacttgatgc aaaccgacga tcgatatgat
    45721 gctatcgatc gtcataaact atacaaactg attatttaat cgcctggtct gaacgctctc
    45781 acctcgtcca gtgtgggcaa cagagaaagt tgttctttca tctcccgctg acagcgataa
    45841 atctcatcgt tgcgctcaac aatggcctgt atcatcgctg tggccagttc ctcaagttcc
    45901 tgcgtcgtca gctgcacctg ctgattatcc gcatcactcc acgtcatatt tgtttgtgct
    45961 gtgacagatt ttgccagcat gactaccggg gacaggcggc ccagtgagtc ggggccagca
    46021 ttccagatac gaccgttcca ttcaaacgtg aacggcttcg cctcctgttc tgcgcgccat
    46081 gcttcaattt cctgacgtct ggcctctctg gccgcttcca gcatttctgg tgtcacagtg
    46141 aatggggcta tctcacccca tttgccgctt tgcagttcct gccagatttg ctgacccgtc
    46201 ggtgcgacat catcagcggt ggctgtgtag gg
//
LOCUS       DAZPEP010000014        46166 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZPEP010000014 DAZPEP010000000
VERSION     DAZPEP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.014"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..716)
                     /locus_tag="ACHUT9_000800"
     CDS             complement(<1..716)
                     /locus_tag="ACHUT9_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1347354.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUT9_000801"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="ACHUT9_000801"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFR1347355.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAVCAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUT9_000802"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="ACHUT9_000802"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFR1347356.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="ACHUT9_000803"
     CDS             complement(3650..4705)
                     /locus_tag="ACHUT9_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1347357.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="ACHUT9_000804"
     CDS             complement(4715..5503)
                     /locus_tag="ACHUT9_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFR1347358.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="ACHUT9_000805"
     CDS             complement(5521..6573)
                     /locus_tag="ACHUT9_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFR1347359.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7446)
                     /locus_tag="ACHUT9_000806"
     CDS             complement(6604..7446)
                     /locus_tag="ACHUT9_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1347360.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSAGNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(7433..8314)
                     /locus_tag="ACHUT9_000807"
     CDS             complement(7433..8314)
                     /locus_tag="ACHUT9_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFR1347361.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8335..9645)
                     /locus_tag="ACHUT9_000808"
     CDS             complement(8335..9645)
                     /locus_tag="ACHUT9_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1347362.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9659..11190)
                     /locus_tag="ACHUT9_000809"
                     /pseudo
     CDS             complement(9659..11190)
                     /locus_tag="ACHUT9_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
     gene            complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHUT9_000810"
     CDS             complement(11402..11716)
                     /gene="pspE"
                     /locus_tag="ACHUT9_000810"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFR1347363.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHUT9_000811"
     CDS             complement(11791..12012)
                     /gene="pspD"
                     /locus_tag="ACHUT9_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFR1347364.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHUT9_000812"
     CDS             complement(12021..12380)
                     /gene="pspC"
                     /locus_tag="ACHUT9_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFR1347365.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHUT9_000813"
     CDS             complement(12380..12604)
                     /gene="pspB"
                     /locus_tag="ACHUT9_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFR1347366.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHUT9_000814"
     CDS             complement(12658..13326)
                     /gene="pspA"
                     /locus_tag="ACHUT9_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFR1347367.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13493..14470
                     /gene="pspF"
                     /locus_tag="ACHUT9_000815"
     CDS             13493..14470
                     /gene="pspF"
                     /locus_tag="ACHUT9_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFR1347368.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHUT9_000816"
                     /pseudo
     CDS             complement(14588..15853)
                     /gene="puuE"
                     /locus_tag="ACHUT9_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHUT9_000817"
     CDS             complement(15891..17171)
                     /gene="puuB"
                     /locus_tag="ACHUT9_000817"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFR1347369.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHUT9_000818"
     CDS             complement(17173..18663)
                     /gene="puuC"
                     /locus_tag="ACHUT9_000818"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFR1347370.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDHAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEEAGLPDGVL
                     NVVSGYGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANI
                     VFADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGH
                     PLDTVTTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNA
                     SLSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSV
                     FVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHUT9_000819"
     CDS             complement(18813..19370)
                     /gene="puuR"
                     /locus_tag="ACHUT9_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFR1347371.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHUT9_000820"
     CDS             complement(19397..20161)
                     /gene="puuD"
                     /locus_tag="ACHUT9_000820"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFR1347372.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20373..21734
                     /gene="puuA"
                     /locus_tag="ACHUT9_000821"
     CDS             20373..21734
                     /gene="puuA"
                     /locus_tag="ACHUT9_000821"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFR1347373.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21883..22047
                     /locus_tag="ACHUT9_000822"
     CDS             21883..22047
                     /locus_tag="ACHUT9_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347374.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            22037..23422
                     /gene="puuP"
                     /locus_tag="ACHUT9_000823"
     CDS             22037..23422
                     /gene="puuP"
                     /locus_tag="ACHUT9_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFR1347375.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23556..23801
                     /locus_tag="ACHUT9_000824"
     CDS             23556..23801
                     /locus_tag="ACHUT9_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFR1347376.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            24114..25757
                     /gene="sapA"
                     /locus_tag="ACHUT9_000825"
     CDS             24114..25757
                     /gene="sapA"
                     /locus_tag="ACHUT9_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFR1347377.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25754..26719
                     /gene="sapB"
                     /locus_tag="ACHUT9_000826"
     CDS             25754..26719
                     /gene="sapB"
                     /locus_tag="ACHUT9_000826"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFR1347378.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26706..27596
                     /gene="sapC"
                     /locus_tag="ACHUT9_000827"
     CDS             26706..27596
                     /gene="sapC"
                     /locus_tag="ACHUT9_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFR1347379.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27596..28588
                     /gene="sapD"
                     /locus_tag="ACHUT9_000828"
     CDS             27596..28588
                     /gene="sapD"
                     /locus_tag="ACHUT9_000828"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFR1347380.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28590..29396
                     /gene="sapF"
                     /locus_tag="ACHUT9_000829"
     CDS             28590..29396
                     /gene="sapF"
                     /locus_tag="ACHUT9_000829"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFR1347381.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29464..29817
                     /locus_tag="ACHUT9_000830"
     CDS             29464..29817
                     /locus_tag="ACHUT9_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFR1347382.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            30185..30973
                     /gene="fabI"
                     /locus_tag="ACHUT9_000831"
     CDS             30185..30973
                     /gene="fabI"
                     /locus_tag="ACHUT9_000831"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFR1347383.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            31118..32244
                     /locus_tag="ACHUT9_000832"
                     /pseudo
     CDS             31118..32244
                     /locus_tag="ACHUT9_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32312..34246
                     /gene="rnb"
                     /locus_tag="ACHUT9_000833"
     CDS             32312..34246
                     /gene="rnb"
                     /locus_tag="ACHUT9_000833"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFR1347384.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34324..34427)
                     /locus_tag="ACHUT9_000834"
                     /pseudo
     CDS             complement(34324..34427)
                     /locus_tag="ACHUT9_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHUT9_000835"
                     /pseudo
     CDS             34481..36471
                     /gene="pdeR"
                     /locus_tag="ACHUT9_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHUT9_000836"
     CDS             complement(36453..36581)
                     /gene="ymiD"
                     /locus_tag="ACHUT9_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFR1347385.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHUT9_000837"
     CDS             36618..36791
                     /gene="yciZ"
                     /locus_tag="ACHUT9_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFR1347386.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36881..37630
                     /locus_tag="ACHUT9_000838"
     CDS             36881..37630
                     /locus_tag="ACHUT9_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFR1347387.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37899..38117
                     /gene="osmB"
                     /locus_tag="ACHUT9_000839"
     CDS             37899..38117
                     /gene="osmB"
                     /locus_tag="ACHUT9_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFR1347388.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHUT9_000840"
     CDS             complement(38243..38569)
                     /gene="yciH"
                     /locus_tag="ACHUT9_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFR1347389.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHUT9_000841"
     CDS             complement(38569..39306)
                     /gene="pyrF"
                     /locus_tag="ACHUT9_000841"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFR1347390.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHUT9_000842"
     CDS             complement(39499..40668)
                     /gene="lapB"
                     /locus_tag="ACHUT9_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFR1347391.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHUT9_000843"
     CDS             complement(40675..40983)
                     /gene="lapA"
                     /locus_tag="ACHUT9_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFR1347392.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHUT9_000844"
     CDS             complement(41132..41896)
                     /gene="pgpB"
                     /locus_tag="ACHUT9_000844"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFR1347393.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            42066..42656
                     /gene="ribA"
                     /locus_tag="ACHUT9_000845"
     CDS             42066..42656
                     /gene="ribA"
                     /locus_tag="ACHUT9_000845"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFR1347394.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLNTKAEKMGHLLNK"
     gene            complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="ACHUT9_000846"
     CDS             complement(42720..45395)
                     /gene="acnA"
                     /locus_tag="ACHUT9_000846"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFR1347395.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKV
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(45559..45654)
                     /gene="ymiC"
                     /locus_tag="ACHUT9_000847"
     CDS             complement(45559..45654)
                     /gene="ymiC"
                     /locus_tag="ACHUT9_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFR1347396.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(45768..45935)
                     /gene="yciX"
                     /locus_tag="ACHUT9_000848"
     CDS             complement(45768..45935)
                     /gene="yciX"
                     /locus_tag="ACHUT9_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFR1347397.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(45938..46078)
                     /locus_tag="ACHUT9_000849"
     CDS             complement(45938..46078)
                     /locus_tag="ACHUT9_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFR1347398.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT        10927 a        12205 c        11625 g        11409 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac agacagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttaccccttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctcaccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc aacccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtgtcg taaatgccca gcgcggtgat gattttgaac agcggcacca ccagcaaaat
     7081 gccagagaac atatacaccg tgtaaaagct ggcgttgatg gtcatccgac ctttaaagcg
     7141 caggcgggaa agcgcgtaag ccccaagaat gccgaggaat accgccacca cggatgaaac
     7201 cacagacacc accagactgt tacggaagta gtcgacaaac gggaaaatca tcgggttaaa
     7261 aatgtcgacg taatgctcca gcgtccactg ctgcggcagc agcgtaggat gcagtgagat
     7321 cgcctctttc gcgcccttga acgaggtcat cagcatcaca aaaaatggaa acagcgtgat
     7381 gatgagaaac agcgccagcc cgcagtaaaa accgatgcga ctgagtgtgc gtttatttgt
     7441 tgccattgag gttcacccgt ttcctggtca gcagaatgac agcgaaaata atgatgaaga
     7501 gcaccaccga aatcgccgcc gctttcccta agtcattaaa ggcaaaggcg gttttgtaga
     7561 gatagacgcc gagaatatcg accttggtgg tcagcagata aacatcagca aacatgtaga
     7621 acatccagat agtgcgcagg gtcaccaccg tcgccaggac cggcataatt gcgggcagcg
     7681 tgacgatgcg aaaccgctgc caggcattag cgccatccat ctctgccgct tcgtacagcg
     7741 atttgtcgat ggtctgcaaa atcgccagaa acgagataaa ggcatacggg aagtagcgcc
     7801 agatggcgaa cagcaccacc agcacaaagc tactgcccgg attatcgaac cacagcggtg
     7861 cctgctcata gagatgcaga agatcgacgc cgaggtagtt cacaatgccg tagccgttgt
     7921 tgaacatgta tttccaggca aacaccagcg aaatggacgg cgttacgtag gagaggatca
     7981 ccagcgaacg cgcggttttg cgcaggcgga actcgcggtt gaaaaacatc gccactgcca
     8041 gcccgagcac ggtgctgccc gccaccacca gcgcggtata ccagaccgtc atccacagcg
     8101 agtgccagaa gccgggatcg gagaggatac gcacatagtt actcacccca acaaaggttg
     8161 actcgatgtt gggattgagc ggcagacgta aaaaactgat ttcgatattc gacaccattg
     8221 gccaggccac cagaccaccc agcagtaata agctgggcgc gagaagcagc agcgcaaagg
     8281 gcatatcgga acgacctgaa aaaagcctgt tcattgcgga ttcctgtcct gggtttagtg
     8341 ctgttcgatc agttcatcca gctttttctg gctcgcttgc agcgtgccag ggagatcggc
     8401 tttacccacg gtgacgttat gcaccatgga gctcaccacg ccagaacccg tcacatcacc
     8461 catgcgggta aagtttttat cccctactgc gccaaagacc tgaatatttg gcagttcacc
     8521 gattagctga ttcggtagtt cacccagcgc cttaataacg tcgttgtctt tccaggtggc
     8581 ggtagtgaca accgctttat tcaccggcag cgcagcacct ggcgacatca tcacccagtc
     8641 ggcaatgttg tctgcctgct ccataaaggt gacaaatttt tctgctgctt ccgtctcttc
     8701 ggccttttgc ccggcggtaa tggtcagcga ggtcaacatg ccgtagaccg cagagttttt
     8761 ctcggttggc accacgaaac cgacgttttt cgggtcgcct tctttaatta cagccggaag
     8821 gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc ataaaggcgt ctttcacttc
     8881 catgatgtcg ttagaacccg gcatgtcgtt agaacccggc atagtgttgg cagcaaggtc
     8941 gcggtaatag gtcagtgcct gcatcatctc tggtgtatca agggtgattt tgccttcggc
     9001 gttaaagacg ttagcctggt tggataacgc aaactgggag aaggattgtt ccgtcaacac
     9061 gctttctgct gtaggcagcg caatgccgta ttttttattc gccgggtcat tcagtttctg
     9121 tgcaacgtcc agtagctgtt gccagttttt cggctcctcc agccccgctt ttgccagcac
     9181 atctttgcga taccagatgc cgccaatcca ggcgctgacg ggaacaccgg tccaggcact
     9241 cccatcttcg gtgcgcacaa tacgcagtac gccatcgtaa aacgcgcctt caccaacgtt
     9301 gctgatgacc gtggcaaccg tcttgcgatc gataagctgc tctttatcca taactttggc
     9361 gtagtcatgg ctggtttcga tcacttccgg cagcgagccg ctacgtgaaa gggtgatgac
     9421 tttggtgtta taagcgtcct cttccacggg cacttgttta acggtgatac ctgggttttc
     9481 tttttcaaaa cgggcaatca atttgctgat aactgcctga cgctcctgct ccaccgaaga
     9541 atgcataaat tcgatggata catgggtttt attttcttct ttacagcctg aaatcagggc
     9601 gcaggaaacc agtgctgata acagcacgat ttttgattta atcatgagat gttccttttt
     9661 atttaatcca cataacctgc caggggcgca gcgttatttc attacccaat agatcaactt
     9721 cactaatcag atcccgacca tgcagattag taatatttac atgctgaata tttttactga
     9781 cattaaacaa tccagtcaga caattaccat cagcgttact tctttgaata cgcattacgg
     9841 atgaattaat cgtatcgatg gtaaaattat tatccggatg aaactcgtta tggctgcgac
     9901 gaagtgtaat taaacgcgac aactcatgat ataccgcatg ccttaatgta gcttcatcgt
     9961 tcagttctcg ggttatctct ttactgtgat attttttacg gttaatcgca cggttatatc
    10021 cgagtttttc gacacctgcg taatcattac gcgagccaag aatactttga atatatatcg
    10081 ctggcacacc ggggaaactt aacaaaatcg catgggcgag aataaacctg gcgcaacgtt
    10141 cttcatcgct actctcacgg cggcttaacg catccatatg tgagaggcga ggaagttaaa
    10201 ccaggtggtg ttgctggaag gtagtgtcag gttttgcgcc cacgcacaaa gcgcctcaac
    10261 gttctgtttt tgcaccgcat gcagcaccag tggcggcagc gagaactggt acaccatatg
    10321 tgcttcgtca tcgcctgcgc caaagtaagc aatgttgtct ttatgcggaa cattggtctc
    10381 ggtaatgatc actgtacctg gcgcaacgtt atcaataatc gaccgtaaca gtttgataat
    10441 cagatgtgtt ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac
    10501 ggcatccaag cggacatact cagcaccttt cgcaaggtaa cagagtaaaa catccaccat
    10561 cgccagcaac acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt
    10621 ccataaatgg cgcgttgaat gatcgcgcat ctggaatggc gttaataacg gtaacgcacg
    10681 cgggcgagtg acggcgctga gatcggtttg cggatcaacc gcaataaaaa aatcttcaaa
    10741 acctggctgc tgttgtaaat agtttttaaa ccattcactt tttgccgaca tatggttgca
    10801 gacaaaatca aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc
    10861 cgcttcactg gcgacctgat gataatcaat taccgaaaac ccatcatcag atgaacatgg
    10921 ataaaaaggt aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtaaaactg
    10981 attaaatgtg ggtaatggtt ttaaatcatt gctgtgaaat tgatcggcat aggtaattaa
    11041 cacgacatca ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact
    11101 ctcaagacgc gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc
    11161 gtccaggtaa tccgtaattt tctgtttcat gttgcctttt tgtggtagcg gtcccatgaa
    11221 acgagagact actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca
    11281 ggaaatcatt ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatggg
    11341 gatgtgaata aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct
    11401 tttaaccttt gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg
    11461 tatatcccat ctcgctaagg atctcttttg cttgccctga ctggcgtccg gcattgcaat
    11521 acactttcac ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct
    11581 tcaggggaat attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa
    11641 catcgatcca gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta
    11701 agcctttttt aaacatgtaa gcgtccttct atggatgaaa attgccatcc attatacgcc
    11761 tgagatttaa caatttttgc gaaactcccc tcacctttta taacgctgtg ccagtttatt
    11821 agcagcccga cttaacagcg gttccagcgc cacagccagc aacattttca gcggtcggcg
    11881 agcaactgat tttatcgccc agcccgctac acccgccggg ccatagcgca gtgcggtaag
    11941 aagtaccagc ttgcctgcta atttgaaacc aggctttacc ttttgcccgg cctgttgcca
    12001 gcgagtattc ataactttcc tcacagttga cggaaacggc tacgtaacgt gaaagtatcg
    12061 gaagtgacat aacgttccat ctcgcgtaaa cgcgtttcac ttgccgccag ttcacggtcg
    12121 acttcatcca gcaattcgct gctggaaggt agctgctcac caaaggccat gttgtccggc
    12181 attggatcaa gcgcaaatga caaaatgatg taagcaacca gggtaaacag cgccagaccg
    12241 aagaaaatcg acagcaccac caggatacgc accagtttta ccggtacatc aaaatagttg
    12301 gcaatcccgg cgcagacgcc gcggaccatg ccctgctgtg gaatacgcca taatttttta
    12361 ttgagattaa tgcccgccat tagcgatccc tccagttcgg atgttctgca tcaagaatag
    12421 attccagcgc ctgaatacgt tcgcgcatcc gttttgcttc atcagccagt tgcgctaatc
    12481 gctgctgctc actttgcgac aattcatcgc gaccagaacg attgctgtaa tgcagccata
    12541 accagatcgg taaaacaaac agcacaaaaa tggttaacgg aatagccaga aatagcgcgc
    12601 tcataagtac tccttagacg atgagcggcg gcgcgttcgg acgccgcctg gatgttatta
    12661 ttgattgtct tgcttcattt tggcttttaa ttgtgccagt tgttcgctga ttgcatcatc
    12721 ggctttcagt tcggcaaact gatcgtccag cgatttttgt ttaccgaagc tgtggctttc
    12781 tgcttccgct tccatctggt caattcgacg ttcgaaagat tcgaaacgag ccattgcttc
    12841 atccagtttg ccactgtcca gctgacgacg cacatcgcgc gacgagtttg ccgcctgatg
    12901 acgtaacatc aatgcctgct ggcgagcgcg tgtttcgctc aatttgtttt ccagctcgcc
    12961 aatctctttc ttcatgcgtg ccagcgtatc gtccaccagc gtcacttcat gttccaggga
    13021 cttaatcaga tcggtcagtt tctgtttttc aattaacgct gcacgtgcca gatcctctct
    13081 ctctttcagc agcgccagtt cggctttttc ctgccattca acttcacgcg ccgacgcttg
    13141 ttcaatacgg cgagtcagct gtttcttttc tgccaacgca cgcgccgaag tagaacgtac
    13201 ttcaaccagt gtatcttcca tctcctggat catcagacgc accagtttct gtggatcttc
    13261 cgctttctct aacagagcgt tgatgttggc attcacgatg tcggcaaagc gagaaaaaat
    13321 acccataatg ttgtcctctt gatttctgcg ttcaggattg tcctgctgaa ctgttaatac
    13381 aatttgcgtg ccaatttttt atctttttga tttataaaga tctgattgaa gaatcaacag
    13441 caacatgcca ggatgaatta gcgaattaca ctaacaagtg gcgaatttca tcatggccga
    13501 atacaaagat aatttacttg gtgaggcgaa cagctttctc gaagtgctgg aacaggtttc
    13561 gcatctcgca ccgctggaca aaccggtgct catcatcggc gaacgcggca ccggtaaaga
    13621 gctgattgcc agccgcctgc attatctctc ctcccgttgg caagggccgt ttatttccct
    13681 taactgcgcg gcgttaaatg aaaatctgct ggattccgaa ctgtttggtc acgaagcggg
    13741 ggcgtttacc ggtgcgcaaa aacgtcatcc agggagattt gaacgtgccg acggcggtac
    13801 gctatttctt gatgaactcg ctacggcacc gatgatggtg caggagaaat tattgcgcgt
    13861 gattgagtac ggtgaactgg agcgcgttgg cggcagccag ccattgcagg tgaatgtgcg
    13921 gttggtatgc gcgacgaatg ccgatctccc ggcgatggtc aatgaaggca cttttcgcgc
    13981 tgacctgctc gaccgactgg cttttgatgt tgtacaactg ccaccactgc gcgagcgcga
    14041 aagcgacata atgttgatgg cagaacactt tgccatccag atgtgtcggg aaatcaagct
    14101 gcctctgttc ccggggttta cggagcgcgc cagagaaaca ttgctgaatt atcgttggcc
    14161 gggaaatatt cgtgaattga aaaacgcggt ggaacgttca gtgtatcgcc acggcaccag
    14221 cgattatccg cttgatgaca tcattattga tccctttaaa cggcgtccgc ctgaagacgc
    14281 tatcgccgtt tcagaaacca cctcgcttcc gacgctgccg ctggatttac gtgagtttca
    14341 aatgcagcag gaaaaagagt tgctgcaact cagtttgcaa aaggggaaat ataaccagaa
    14401 gcgcgcagct gaattgctgg ggttaaccta ccatcagttc cgcgcgttgt tgaaaaaaca
    14461 ccagatttag cgaagttggg caatgcgatt cgccggatgc ggcgtgaacg ctttatccgg
    14521 catacataat caacgtcgtt tgcaggcctg ataagcgcag cgcatcagac attattgcgt
    14581 tgggctatta atcgctcagc gcatcctgca aaattttcat tgccgcatcg aattgcgcat
    14641 ccgggatggt cagcggatac aggaagcgaa tcacgttgcc gtatgcgcca caggtcagca
    14701 ggagcagccc ctgcgccagc gcgcgttgct ggattttctg tgcaatcgcc gctgacggct
    14761 cgcccgtttg cggatcgtta aactctgccg caatcatcga ccccaggccg cgtaccgcag
    14821 caatggccgg aacgctttct ttggcatcaa tcaacgtgtt tttgagacgc tggcccagtt
    14881 gattcgcgcg ttcgcagagt gattctttgt tgataatatt gagcaccgcg tgcgcggcag
    14941 ccaccgccag cgggttcccg gcgtaggtgc cacccaaccc gcccggcgcg ggcgcgtcca
    15001 taatattcgc gttaccgacc acgcccgaaa gcggcatccc gcccgcgagg cttttcgcca
    15061 tcgtcattaa gtccggctta tcggcgtaat gatccatggc aaacagttta ccggtacgcg
    15121 caaagccgct ttgcacttca tcggcaatca tcacaatacc gtgctcgtcg cacaggcggc
    15181 gaatggcggc aaccagctct tttggcgcaa cgttgaaacc gccctcgccc tgcaccggtt
    15241 cgaaaataat ggccgcgacc tgcttcgctt cgatgtctga tttaaacaag cgttcgatgg
    15301 cgtcgaggga gtcctatgtt gaaatgccgt gtaaatctga cggataaggt acgtgataca
    15361 ccgaaccagg gaacgggccg aagccgattt tgtacggcgc aacttttccg gtcagcgcca
    15421 tggtcatata tgtacgaccg tggaagccac cgctaaacgc aatcacgcca gggcgtccgg
    15481 tatgggcgcg agcaattttc accgcgtttt ccaccgcttc cgcaccggta gtgaagaacg
    15541 cggttttggc ctgcccgctc accggggcaa gggcgttgat tttctccgcc agggtgacgt
    15601 agctttcata cggcacaatc tgatacgcgg tgtgggtaaa ctgttgcagt tgctgctcca
    15661 ccgccgcgac cagatcaggg tggcgatgtc cggtattcag caccgcaatg cctgcggcga
    15721 aatcgatgta ctcgttgccc tcaacatcct tcagagtagc gttttcagcc gactgggcga
    15781 agaagttaca catcacgcca accccgcgtg gggtagcaga aagacgacgc tgatggaatt
    15841 cattgttgct catgtttcac accttttgac tcttcactgt taatcggaaa ttaaacgccc
    15901 agacgatcgc gcaggctgta ccaggctgcg cccatcgcgg taaacggtac tcgcagcgtg
    15961 cgcccgccgg ggaacgggta atacggcaga ttggcgaagg catcgaaacg ttcggcgtcg
    16021 ccgcgcagca gttcggcaat caaacgtccg gcgagatgag tacaggtgac gccgtggccg
    16081 ctgtaaccct gcatgtaata gatgttggta tcaaggcgac caaactgcgg catgcgcgac
    16141 agggtcagca ggaagttgcc cgtccagcgg tagtcgattt tgacgccctt cagttgcggg
    16201 aaggttttca gcagtttcgg cacaacaaga cgctcaacgt catccgggtc gcgcgcgccg
    16261 tagaccacgc cgccgccgta cagcaaacgg ttgtcggcgg taagacggta gtaatcaagc
    16321 agataattac agtcttccac acagtagttt ttcgggatca gcgaacgggc taaatcttcc
    16381 gacagccgtt cagtggtgat cacctgcgtg ccgcacggca tgctgcgttt cgccagttcc
    16441 ggctctactt tatcgcccag atacgcattc ccggcgacaa tcacatactt cgccgtcacc
    16501 tgacctttgg cagtgcgcac caccgctggc gtggtgtgct ggatctgcgt cacggcagaa
    16561 agctcataca cgcgcccgcc gttcaggcgg atggcatcgg cttcaccaat ggcgaggttt
    16621 agcgggtgaa tatgcccacc gctgtgatcc aacagtgcgc cagtgtagcg atcgctggcg
    16681 acctcgcggc gaatggcgtt agcgtccagc agctccagct gtttattgcc gtagcgttcc
    16741 cagttctctt tctgctcttc gagtgtggcg agctgtttat cattcatcgc cacaaacagg
    16801 ccgccggggc ggtagtcgca gtcaatctga tagcgtttga tacgttcgcg gatgatctcg
    16861 ccaccctcga acatcatgct gccgagcata cgggcggtgt ccatgccgta gcttttttcg
    16921 atcacgtcga tgtcgcggct gtaggagttc acaagctgcc cgccattgcg cccgcttgcg
    16981 ccaaagccga tgcgtgaggc ttcgagaacc actacgtcaa agcccgcttc cgccagatgc
    17041 agcgcggagg agagcccggt atagccgccg ccaaccacgc aaacgtcgca ggtgattgac
    17101 tcattcagtg tgtcgaatgg tgcatattta ttcgcactgg cggcgtagta actgctggta
    17161 tgttcggtca tttcaggcct ccaggcttat ccagatggtt ttcagttcag tgaatttttc
    17221 aagggcatgc agggatttgt cgcgaccgtt gccgctctgc ttatagccgc caaacggcac
    17281 ggtcatatcg ccgtcgttgt agttattgac gaagacggaa ccggctttca ggcggcggct
    17341 catacggtgc gcgcgggaga ggtcgcgcgt ccataccgcc gcgccaaggc cgtactggct
    17401 gtcgttggca agctgtagcg cctggtcttc tgatgtgaaa cgcgtgacca ccagcaccgg
    17461 accgaaaatc tcttcgcggc ttaaggacgc attcgggtca acatccacaa agatggtcgg
    17521 gccgatggcg gcagccagct cagcgttacg accatccagc agcagttgcc ctttgctttc
    17581 gccttcacga ataaagctat ggaccgagtc ggcgtgggcg caatcgatta aggtgcccat
    17641 ggtggttacg gtatcaagtg gatggcccgg ctgccagttt tgcgcctgct gttttaacag
    17701 ggctaagaat tcatcggcga tgctctcttc cagtaacaaa cgcgttccgg cgatgcacac
    17761 ctggccctgg ttgtagaaga tgcctgcggc ggtggcgctt gccgcttttt gcagatccgg
    17821 acaatcggcg aaaacgatgt tggcgctttt gccgcccgct tccagccaga cgcgtttcat
    17881 gttgctatca cccgcgtctt tcagcagttg tttacccgta cgcgtggagc cggtaaaggc
    17941 gatggcatcg atatcgttat gacgcgaaag cgcctgcccg gcttcatgac cataaccgct
    18001 cactacgttc agcacgccat ccggcagccc cgcttcttct tttgccagcc cggcgagacg
    18061 aatcgcactg agtggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag
    18121 cgccgggccg agtttccagc aagtcagcag cagcgggaag ttccacggca cgatggcggc
    18181 aatcacgccg accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac
    18241 ttcgccatac actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg
    18301 aatatcatca cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc
    18361 cagctcttcg gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt
    18421 agccggagaa gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc
    18481 atggtcgata tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg
    18541 atcaacggtt tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa
    18601 gcggttttca atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt
    18661 cattatgact cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag
    18721 agatccccgg gccgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc
    18781 attgcgctta cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg
    18841 ataattcggc aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg
    18901 gcataacttt gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca
    18961 ccttccagta ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg
    19021 cccggctggt acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc
    19081 agcttcattg acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc
    19141 tgcggctcat caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc
    19201 ttcagcagct tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg
    19261 atagcactgt gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc
    19321 tggcggattt ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt
    19381 ccttaaactg tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga
    19441 taaagccctc gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc
    19501 actgtacgcc cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg
    19561 gcgagcgagc ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag
    19621 agtttaccca aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa
    19681 cctgaacttc atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat
    19741 gttccagcaa ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca
    19801 ccagttcttg taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat
    19861 taattatcgc catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt
    19921 caccatatag gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt
    19981 tcggcaaaag ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca
    20041 agccgcctgc gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac
    20101 ccttaagcct gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca
    20161 tgctcaatct cacaaagtgg actaaattat cgccattact gcgctttaaa tgacctttat
    20221 gttcaatatt ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacattt
    20281 gacaaacatt tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata
    20341 acgagcggaa aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga
    20401 actttgttca gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct
    20461 acctggagcg ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt
    20521 gcttccgtgg aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt
    20581 tcccggcctc ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg
    20641 gtcaggaaat gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt
    20701 ctgccgccga tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg
    20761 ctccctttga cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc
    20821 gcgacgctga agggaatctg caaccgccct gcgcgcctgg caccgatgac cgcaatacac
    20881 aaagccaggt ttactccgtt gataacctca accacttcgc cgacgtgctc aatgatattg
    20941 atgaactggc gcagttgcag ctgattccgg cagatggcgc ggtcgctgag gcctcgccgg
    21001 gtcagtttga aatcaacctt taccatactg ataacgtgct ggaagcctgc gatgatgcgc
    21061 tggcactaaa acgcctggtg cgtctgatgg caggaaagca taagatgcac gccactttta
    21121 tggcgaagcc gtatgaagag cacgcgggca gcgggatgca tatccatatc agtatgcaaa
    21181 ataatcgtgg cgagaatgtg ctttctgaca cagaaggcga agattcgccg ctgctgaaaa
    21241 agatgctcgc cgggatgatt gacctgatgc cgtcgtcgat ggcgttgctg gcaccaaacg
    21301 tgaactcgta tcgccgcttc cagccgggaa tgtatgtgcc gacgcaggcg tcgtggggcc
    21361 ataacaaccg caccgtcgcc ctgcgtattc cgtgcggcga ccgtcataat caccgcgtgg
    21421 aatatcgcgt ggcgggtgcc gatgccaacc catatctggt gatggcagcg atttttgccg
    21481 gtattttgca tggccttgat aacgagctgc cgttgcagga agaagtcgaa ggcaacgggc
    21541 tggaacagga aggcttaccc ttcccgattc gccagagcga tgccctgggt gagtttatcg
    21601 agaatgatca cctgcgccgc tatttaggca aacggttttg ccatgtgtat cacgcctgta
    21661 aaaatgatga gctgttgcag tttgagcgcc tcatcacaga aacagaaatt gagtggatgt
    21721 tgaaaaacgc gtaattcccc ttgcggggtt gtttcatcgg cctgagcgga gccaggcctg
    21781 acatttgctg gtaatccaga catttcccgg atgtcgcgtt atggagcgca ggcggcaacg
    21841 ggcttccgtt aacgattaag actgtgacga ggaaatgaga tgatgccgat gattaaatcc
    21901 ccgcatggcg agggaggctg cgtatgcgcc cctcctgcta ctgactggac tccgccccct
    21961 ttgcttccct tgctaaacag gtttgatttc aggtcgacac gaccgcaaac cttattacgc
    22021 aggggaggca gcaattatgg ctattaattc accactgaat attgctgcgc aacccggcaa
    22081 aacccgtctg cgaaaatcac tgaaattgtg gcaggtggta atgatgggtc tggcctatct
    22141 cacgccgatg accgtatttg atacttttgg tattgtctcc ggcattagcg acggtcacgt
    22201 tccggcgtcc tatttgctgg cgctggcggg cgtgctgttt accgctatca gctacggcaa
    22261 actggttcgc cagtttccgg aggccggttc ggcctatacc tacgcgcaaa agtcgattaa
    22321 cccgcacgtc ggatttatgg tcggctggtc atcactgctg gattatctct ttttgccaat
    22381 gatcaacgtt ttgttggcga aaatctatct ctccgccctc ttcccggaag tgccgccgtg
    22441 ggtgtgggtg gtaaccttcg tcgccatttt aaccgccgcg aatctgaaga gcgtcaacct
    22501 ggtcgctaac ttcaataccc tgtttgtact ggtgcaaatc tccatcatgg tggtgtttat
    22561 cttcctggtg gttcagggac tgcataaagg agaaggcgtt ggcaccgtct ggtcacttca
    22621 gccgtttatc agcgagaacg cgcacctgat cccgattatt accggtgcga cgattgtctg
    22681 tttctcgttc ctcggtttcg atgcggtgac cacgctttcg gaagagacgc cggacgccgc
    22741 acgcgtgatc ccgaaagcca tcttcctgac ggcggtatac ggcggcgtta tctttatcgc
    22801 ggcgtcgttc tttatgcagc tgttctttcc cgatatcagc cgctttaaag acccggacgc
    22861 cgcactgcct gaaattgcgc tgtacgtcgg cggcaagctc tttcagtcga ttttcctctg
    22921 caccacgttt gtgaacacat tagcgtctgg cctggcttca catgccagcg tgtcgcgtct
    22981 gctgtatgtg atggggcgtg acaatgtgtt tccggagcgc gtgtttggct atgtgcaccc
    23041 aaaatggcgg actccggcac tcaacgtcat tatggtcggg attgtcgcgc tgtcggcgct
    23101 gttctttgat ttagtcaccg cgacagcact gattaacttc ggtgctctgg tggcgtttac
    23161 cttcgtgaat ctgtcggtgt ttaatcattt ctggcggcgt aaaggaatga ataaaagctg
    23221 gaaggatcac ttccactatt tgctgatgcc gctggttggc gcgctgacgg tgggtgtgct
    23281 gtgggttaac ctagagtcaa cgtcactgac actcggtctg gtatgggctt cgttaggcgg
    23341 cgcatatttg tggtatctga tccgccgcta tcgcaaagtg ccgctgtacg aaggtgacag
    23401 aacgccggtg agcgaaacgt aatagccgga tgcgcggcgc attatcctgc ctgagggtaa
    23461 cgcgccgcgc cattaactat aaaaagatgt atgacccgga tcacgagtgc tatcatccgc
    23521 ccttatgctt agcgcattaa ccccaggggc aaataatgaa ccacgatatc ccgctaaaat
    23581 attttgatat tgccgatgag tacgcgaccg aatgcgcaga gcctgtcgca gaggctgaac
    23641 gcacaccgct ggcccactac ttccagttac tgctcacccg tttgatgaac aatgaagaga
    23701 tcagcgaaga agcccaacat gaaatggctg ctgaagcggg gattaatccc gtacgcattg
    23761 atgagatcgc agagtttttg aatcaatggg gtaatgagta attatttaat tgcagacgat
    23821 cggtcagtat cgttgtccgg ataaagcgtt cacgccgcat ccggctaaca atgacacgca
    23881 ctgcagcgtg ctttaatggg tttctcacga tcgtaccgct ctgctcctcg ctaagttgtt
    23941 ctgtgagtta tagttataga gaaatcacgg gtacgaatgt tgaacagatg gcgacgccac
    24001 ctgttcaacg accaaccaca cggccctcat tacgacccct gaacacaaac ggtaatggca
    24061 aatgtcatta aatattagta agatagcctc tttacataaa atcccttaat attatgcgcc
    24121 aggtattatc gtctcttttg gtgattgctg gacttgtgag tggtcaggca atcgccgcgc
    24181 ctgaatcccc cccgcatgct gatatccgcg acagcggttt tgtctattgc gtcagcgggc
    24241 aagtcaacac ctttaaccca tccaaagcga gcagtgggtt aattgtcgat acccttgccg
    24301 cccagtttta tgatcgactg ctggatgtcg atccctatac ctatcgcctg atgccggaac
    24361 ttgccgaaag ctgggaagta ctcgacaacg gcgcgaccta tcgcttccac ctgcgtcgcg
    24421 atgttccgtt tcaaaaaacc gactggttta ctcccactcg taaaatgaat gccgacgatg
    24481 tggtgtttac cttccagcga atttttgacc gcaacaaccc gtggcataac gtcaacggca
    24541 gcaacttccc ctacttcgac agcctgcaat ttgccgataa cgtgaaaagc gtccgcaaac
    24601 tggataatca taccgttgag ttccgactcg cccagccgga tgcttctttt ttgtggcacc
    24661 tcgcaaccca ttatgcttcg gtcatgtcgg cagaatatgc ccggaagtta gagaaagaag
    24721 atcgccagga gcaactcgac cgtcaaccgg tcggcaccgg tccgtatcag ttgtcggaat
    24781 accgcgccgg gcaatttatt cgcctacaac gtcatgatga cttctggcgc ggtaaaccgt
    24841 taatgccgca ggtagtggtg gatttaggct ccggcggcac cggacgtctg tcgaaactcc
    24901 tgaccgggga atgcgacgtt ctggcctggc ctgctgccag ccagctatcc attttgcgtg
    24961 acgacccgcg cttgcgttta acgctgcgtc ctgggatgaa cgtcgcctat ctggcattta
    25021 acaccgccaa accgccgcta aataatcccg ctgtccgcca tgcactggca ctggcgatta
    25081 ataaccagcg cctgatgcaa tccatctatt atggtacggc tgaaacggcg gcctctattt
    25141 taccgcgcgc ctcgtgggcc tatgacaacg aggctaaaat tactgaatac aatccggcga
    25201 aatcgcgcga acagttgaag gcgttggggc tggaaaactt aacgctgaaa ctgtgggtgc
    25261 ccacacgttc gcaggcgtgg aaccccagtc cactgaaaac tgccgaactg attcaggcgg
    25321 atatggcgca ggttggcgta aaagtggtga ttgtgccggt agaaggtcgc tttcaggagg
    25381 cgcggttgat ggatatgagc catgatctga cgttatccgg ttgggcgacg gacagtaacg
    25441 acccggacag tttcttccgt cctttactga gctgcgcggc aattcattca cagaccaacc
    25501 tcgcccactg gtgcgatccg aaattcgaca gcgtgttgcg taaggcgctc tcctcgcagc
    25561 agctggcggc gcgtattgaa gcctatgacg aagcgcagag tattctggcg caggaattac
    25621 ctattctgcc gctggcgtcg tcattgcgtt tgcaggccta tcggtacgat atcaaaggtc
    25681 tggtacttag cccgtttggt aacgcctcct ttgctggggt gtatcgcgag aaacaggatg
    25741 aggtgaaaaa accatgatta tcttcacctt acgccgcatt ttgttattga ttgtcacctt
    25801 gttcctgctg accttcgttg gcttcagctt gagctatttc acccctcatg cgccgttaca
    25861 aggcgcgtca ctgtggaatg cctgggtgtt ctggtttaac ggcctgatcc actgggattt
    25921 tggtgtttcc agcattaacg gtcaaccgat tgccgaacag ttaaaagagg tcttcccggc
    25981 gacgatggaa ctgtgcatcc tcgccttcgg ttttgcgctg attgttggca tccccgtggg
    26041 gatgattgcc gggattacgc gccataagtg gcaggataac ttgatcaacg ccatcgcctt
    26101 actgggcttt tcaatcccgg tgttctggct ggcgcttctg ttgacgctgt tttgttcact
    26161 cacgctaggt tggttgcccg tttcagggcg tttcgatctg ctctacgaag tgaaaccgat
    26221 taccggtttt gcgttgattg atgcctggct ttcggactca ccatggcggg atgaaatgat
    26281 catgagcgca atccgccata tgatattgcc cgtgatcacc ctgtcggttg cgccaacaac
    26341 tgaagtgatc cgcctgatgc gtatcagcac tatcgaagtg tatgaccaga actatgtaaa
    26401 agcggcggcg acccgcggtt tgtcgcgctt tactattttg cgtcgccacg ttctgcataa
    26461 cgcgttaccg ccggttattc ctcgcctggg cttacagttt tccaccatgc tgacgctggc
    26521 gatgatcacc gaaatggttt ttagctggcc gggtttaggg cgctggttaa ttaacgccat
    26581 ccgccagcag gattacgcag ccatttccgc cggagtgatg gtgtgtggct cactggtcat
    26641 tattgttaac gtgatttctg atattttggg tgccatggct aaccctctga aacataagga
    26701 atggtatgcc ttacgatagc gtatacagcg aaaagcgccc gccgggcacg ctgcgtaccg
    26761 cctggcgcaa attttatagt gacgcctctg cgatggtcgg cctgtacggc tgcgcggggc
    26821 tggctgtact gtgtattttt ggcggctggt ttgcgcctta cggcatcgac cagcaatttc
    26881 tcggttatca attattgccg ccatcatggt cgcgctatgg cgaagtttct ttcttcctgg
    26941 ggactgacga cctcggacgc gatgttttaa gccgtttact gagcggagct gcgccaaccg
    27001 tgggtggcgc atttgtggtg acgcttgccg cgacgatctg tggcctggta ctgggcacct
    27061 tcgccggggc gacacacggc ctccgctcag cggtgcttaa ccatattctg gataccttac
    27121 tggcgatccc ttcgctgctg ttggcaatta tcgttgtagc gtttgccgga ccgagtttgt
    27181 cccacgctat gtttgctgtc tggctggcat tgctgccgcg tatggtacgt tcgatttaca
    27241 gcatggtgca tgacgaactg gaaaaagagt acgttatcgc cgcccgtctg gatggcgcat
    27301 caacgctgaa tattctctgg tttgctgtga tgccaaacat caccgccggg ctggtcaccg
    27361 agatcacccg cgcactgtcg atggcaattc tcgatatcgc cgcgctgggc tttctcgatc
    27421 tcggcgcaca actcccctcg cctgaatggg gagcgatgct cggtgatgcg ctggaactga
    27481 tttatgtcgc cccgtggact gtcatgctgc caggtgcggc aattatgatt agcgtgttgt
    27541 tggttaacct ccttggtgat ggcgttcgtc gtgcgattat tgcgggggtg gaataatgcc
    27601 attactcgat attcgtaacc tgaccattga atttaaaacc ggtgatgagt gggttaaagc
    27661 cgtcgaccgg gtaagtatga ccttaaccga aggtgaaatc cgcggtcttg ttggtgaatc
    27721 cggttccggc aagagtttga ttgcgaaagc gatttgtggg gtgaataaag ataactggcg
    27781 tgttactgct gaccgtatgc gttttgatga tatcgatttg ctgcgtctct ccgcacgcga
    27841 acggcgcaaa ctggttggcc ataacgtgtc gatgattttc caggaaccgc agtcgtgtct
    27901 tgacccttca gaacgtgtgg gtcgccagtt gatgcaaaac atcccagcct ggacctataa
    27961 aggccgttgg tggcagcgtt ttggctggcg caaacgccgt gccattgaac tgctgcaccg
    28021 cgtggggatt aaagatcaca aagatgcgat gcgcagtttt ccctatgagt tgaccgaagg
    28081 tgaatgtcag aaagtgatga tagccattgc actggcgaat caaccgcgtc tgctgattgc
    28141 tgacgaaccg accaactcaa tggagccaac aacccaggcg caaatctttc gcctgctgac
    28201 gcgtctcaac caaaacagca ataccactat tttgcttatc agccatgact tacaaatgct
    28261 tagccaatgg gcggataaaa ttaacgtgct ttactgcggt caaacagtgg aaaccgcgcc
    28321 gagtaaggag ttggtgacga tgccacatca tccttatacc caggcgctga tccgcgcgat
    28381 accagacttc ggcagcgcga tgccgcataa aagtcgcctc aatacgctgc ccggcgctat
    28441 cccactgctg gaacagttac cgattggttg tcgtctgggg ccacgttgcc cgtatgcaca
    28501 acgagaatgc attgtgacgc cacgtttgac gggggcgaaa aatcatctct atgcctgtca
    28561 tttcccgctg aacatggaga aagagtgaga tgatcgaaac gctgcttgaa gtgcgtaatt
    28621 taagtaaaac gttccgctac cggaccggct ggtttcgtcg tcagaccgta gaagcggtaa
    28681 aacccttgag ctttacgcta cgtgaaggcc agacactggc gattattggc gagaatggtt
    28741 cgggtaaatc cacgctggca aaaatgctgg cgggaatgat agagcctacc agcggcgagt
    28801 tattgattga cgatcatcca ctgcattttg gcgattattc cttccgcagt cagcgtattc
    28861 gcatgatttt tcaggatcct tcgacctcgt tgaatccccg tcaacgtatc tcgcaaattc
    28921 tggattttcc actgcgcctg aacaccgacc tggaaccgga acagcgtcgc aaacagatta
    28981 ttgaaacgat gcgtatggtt gggctgctac cggatcacgt cagttactat ccgcatatgt
    29041 tggcacccgg acaaaaacag cgtctgggtc tggcgcgcgc gctaatattg cgcccaaaag
    29101 tcattattgc cgatgaagcc ctcgcttcac tggatatgtc gatgcgttcg cagttgatta
    29161 atctgatgct ggagttacag gaaaaacagg gcatttcgta tatttatgtt acccagcata
    29221 tcggaatgat gaagcacatt agcgaccagg tgctggtgat gcatcagggc gaggttgtcg
    29281 agcgaggcag caccgcggat gtgctggcat cgccgctgca tgaactcacc aaacggctga
    29341 ttgccgggca ctttggcgaa gcattgacag cggatgcgtg gcgtaaagat cgctaatttt
    29401 ctgaatcctc cttccagatt atccacctca ttctgacaag agattaaatc ttgtcagaat
    29461 gaggtgatgg ataaaataaa atcaaatgcg cgtgatttaa gacgcaatct cactttgcag
    29521 gaacgaaagc tctggcgata tcttcgcagc cgacgttttg gtgatttcaa atttcgccgt
    29581 caacatccgg tggggagcta cattctcgat tttgcttgct gctcggcgcg cgtagtcgtt
    29641 gagctggatg gtgggcagca tgatttggca gttgcctatg ataccaggcg cactagctgg
    29701 cttgagtcgc agggctggac cgtgctgcgt ttctggaata acgagattga ttgtaatgag
    29761 gaggcggtgc cggagattat tctgcaggaa ctgaaccgcc gatcaccctc accctgaaag
    29821 ggcgaggggg ccgaccgagc cgaatagctg ttgtggtgaa aacatggaga cagtgctgga
    29881 gaatattcgg caaggtctga accatcccag ccatcgccat aaaagggtta ggggctgtat
    29941 gagcctgttt gttgctgggg taacaatatt tgcacaatat ggtcccctcg cccctctggg
    30001 gagagggtta gggtaagggg aaaagcgccc accctgccgc agcctgctcc ggtcggacct
    30061 ggcaactata gctactcaca gccaggttga ttataataac cgtttatctg ttcgtactgt
    30121 ttactaaaac gacgaatcgc ctgattttca ggcacaacaa gcatcaacaa taaggattaa
    30181 agctatgggt tttctttccg gtaagcgcat tctggtaacc ggtgttgcca gcaaactatc
    30241 catcgcctac ggtatcgctc aggcgatgca ccgcgaagga gctgaactgg cattcaccta
    30301 ccagaacgac aaactgaaag gccgcgtaga agaatttgcc gctcaattgg gttctgacat
    30361 cgttctgcag tgcgatgttg cagaagatgc cagcatcgac accatgttcg ctgaactggg
    30421 gaaagtttgg ccgaaatttg acggtttcgt acactctatt ggttttgcac ctggcgatca
    30481 gctggatggt gactatgtta acgccgttac ccgtgaaggc ttcaaaattg cccacgacat
    30541 cagctcctac agcttcgttg caatggcaaa agcttgccgc tccatgctga atccgggttc
    30601 tgccctgctg accctttcct accttggcgc tgagcgcgct atcccgaact acaacgttat
    30661 gggtctggca aaagcgtctc tggaagcgaa cgtgcgctat atggcgaacg cgatgggtcc
    30721 ggaaggtgtg cgtgttaacg ccatctctgc tggtccgatc cgtactctgg cggcttccgg
    30781 tatcaaagac ttccgcaaaa tgctggctca ttgcgaagcc gttaccccga ttcgccgtac
    30841 cgttactatt gaagatgtgg gtaactccgc ggcattcctg tgctccgatc tctctgccgg
    30901 tatctccggt gaagtggtcc acgttgacgg cggtttcagc atcgctgcaa tgaacgaact
    30961 cgaattgaaa taatcattct gttggtaaat attgggcggc gttctgccgc ccgttatctc
    31021 tgttatacct ttctgatatt tgttatcgcc gatccgtctt tctccccttc ccgccttgcg
    31081 tcaggataac gatttccttt acgaccaagg agcgcccatg gaacaacgcc acatcaccgg
    31141 caaaagccac tggtatcatg aaacgcaatc cagtactgcg gagtatgacg ttctgcctct
    31201 ggtcccggaa gccgcaaagg tcagcgatcc ctttctgctc gacgtgatcc ttgatgaaga
    31261 aacgctggct cccttccttt catggctggt ccctgcgcgc gttcttgcag tggaattgtt
    31321 ccctgaccag cttaccgtga cccgttcaca gaccttcacc gcttatgaac gcttgtctac
    31381 ggccctgacg gttgctcagg tttgcggcgt ccagcggtta tgtaactact attcggcgcg
    31441 acttacgccg ctccccgggc ctgattcctc cagggaaagc aatcataggt tggcacaaat
    31501 cacgcaatat gcccgccaac tggctagctc gccttctatt atcgacaacc gatcgcgcca
    31561 gcatctgaat gacgtcggtc ttactgcctg ggactgtgtg atcattaacc aaatcattgg
    31621 ttttattggc tttcaggcgc ggaccattgc gacatttcag gcttatctcg ggcacccggt
    31681 acgctggtta cccggtctgg aaatacaaaa ctacgccgac gcgtcactgt ttgctgatga
    31741 atcaatacgc tggcgaagca gctatgaggt ggaaaaacta cctgaagagt acacaaaaag
    31801 ttcaacagca gaactttgcc aactggccga aacactctct ctccacccta tttcactttc
    31861 ccttctcgaa aggttgttaa acagtacacg ggttaataca cagccggata ataagcttgc
    31921 ggcgttgtta tgcgcgcgga taaatggcag tcctgcttgt tttgccgcct gtatggattc
    31981 atcaaatgaa tataaaaaat cagccccctt ctgcgcaagg gcgaaaatga aattaaccaa
    32041 tgggctgacc gtcattctgt tgagcacgct accgttcagg cgatacaatg gctgacccga
    32101 gcacccgatc gctttagcgc cgcccagttc agccctttac tcgaacacga aaaatcatca
    32161 acgcagatta ttaatctgct ggtatggagc gggctgtgtg gctggataaa tcgcttaaaa
    32221 atcgcgttgg gtgagacata ttaaccttgc cgcgtcagac agattcgcgt aaaactgtca
    32281 gccgctctaa tggccaccaa aatagacaat tatgtttcag gacaacccgc tgctagcgca
    32341 gcttaaacag caactgcatt cccagacgcc acgcgctgaa ggggtggtaa aagccacaga
    32401 aaaaggcttt ggcttcctgg aagtcgacgc gcaaaaaagt tatttcattc cgccgccgca
    32461 gatgaaaaaa gtcatgcatg gcgaccgaat tatcgcggtg atccacagtg aaaaagaacg
    32521 tgaatccgca gagccagaag aactggttga accgttcctg actcgtttcg tgggtaaagt
    32581 tcagggcaaa aatgaccgtc tggccatcgt tcctgatcat ccactcttaa aagacgccat
    32641 tccttgccgc gcagcccgtg gcctgaacca cgagtttaaa gaaggcgact gggcggttgc
    32701 cgaaatgcgc cgtcatccgc tgaaaggcga tcgttctttc tatgctgaac tgacacaata
    32761 catcactttt ggtgacgacc actttgtacc gtggtgggtt acccttgcgc gccataatct
    32821 ggaaaaagaa gcaccagacg gcgtcgctac cgaaatgctc gatgaaggtc tggttcgtga
    32881 agatctgacc gcgctggatt ttgtcaccat cgacagtgcc agcacagaag atatggatga
    32941 cgcccttttc gctaaggcgt tgccggatga caaacttcag ctgattgtgg cgattgccga
    33001 tccaaccgcg tggattgctg aaggcagcaa gctggacaaa gccgcgaaaa ttcgcgcatt
    33061 caccaactat ctgcctggct tcaacatccc tatgcttcct cgcgagcttt ctgacgatct
    33121 ctgctcactg cgcgccaatg aagtccgccc ggtactggca tgccgcatga cgctctccgc
    33181 tgatggcacc attgaagata atatcgaatt ctttgccgcc accatcgaat ccaaagcgaa
    33241 gctggtgtat gaccaggttt ctgactggct ggaaaatacc ggtgactggc agcctgaaag
    33301 tgaagcaatt gccgaacaag tccgtttgct agcgcaaatt tgccaacgcc gcggcgagtg
    33361 gcgtcataac cacgcactgg tgtttaaaga tcgcccggat taccgcttta ttctcggtga
    33421 aaaaggtgaa gtgctggata tcgtcgccga gcctcgtcgc attgccaacc gtatcgtcga
    33481 agaagcgatg attgccgcta acatttgtgc ggcccgcgtt ctgcgcgata agctcggttt
    33541 tggcatctat aacgtgcata tgggctttga tccggcgaat gccgacgcgc tggcagcgtt
    33601 gctgaaaacg cacggtctgc atgtcgatgc cgaagaagtg ctcacgctgg acggtttctg
    33661 caaactgcgt cgtgaactgg atgcgcaacc aactggtttc ctcgacagcc gcattcgtcg
    33721 cttccagtca tttgctgaaa ttagcactga acccggtcct cactttggcc tcggtctgga
    33781 agcatacgcc acctggactt cgccgatccg taaatatggc gacatgatca accaccgtct
    33841 gctgaaagcg gttatcaaag gcgaaactgc gacgcgtccg caggatgaaa tcactgtcca
    33901 aatggccgaa cgtcgccgtc tcaaccggat ggcagaacgt gatgttggcg actggttata
    33961 cgcacgcttc ctgaaagata aagccgggac cggcacccgt ttcgcggcgg aaattgtcga
    34021 tatcagccgt ggcggcatgc gtgtgcgttt ggttgataac ggcgctatcg cctttattcc
    34081 ggcacctttc ttacacgctg tgcgcgatga actggtttgc agccaggaaa acggcactgt
    34141 acaaattaaa ggtgaaacgg tttacaaagt aactgacgtt attgacgtca ccattgccga
    34201 agtccgcatg gaaacccgcg gcattattgc gcgcccggtc gcgtaatctc ctttcacggc
    34261 ccattcctca tggatgggcc gtttatttcc ccgctctacc ttcgttatat ccaggcagat
    34321 tttttaactg actttcgttt gaaaactggc gtttttccaa aactggatta caaataaata
    34381 tataaaaaaa caatgcgttt agccgccgtt aatttcatgt aagtcatgtt gttcctttta
    34441 tttaaccgcg gactccgcag ttaaccggag gatatgcatc atgaaaaccg ttagggagtc
    34501 cacaacgttg tacaactttc tcggatcgca caatccatac tggcggttga cggaaagcag
    34561 cgatgttttg cgcttttcta ccaccgaaac cacagaacct gatcgtactt tgcagttatc
    34621 tgccgaacag gctgctcgca tcagggaaat gacggtcatc acctccagcc tgatgatgag
    34681 tctgaccgtc gatgagagcg atctttctgt gcatctggta ggacgaaaaa tcaataaacg
    34741 ggaatgggct ggcaacgcgt ctgcatggca tgacacaccg gcagttgctc gtgatttatc
    34801 acacgggctt tcctttgctg agcaggtagt ttctgaagca cattccgcaa tagtgattct
    34861 cgacagccgg gggaatatcc aacgcttcaa tcggttatgt gaagattaca ctgggttgaa
    34921 agaacacgac gtcattggcc aaagcgtgtt taaactgttt atgagccgtc gtgaagctgc
    34981 ggcatccagg cgcaataacc gtgtattttt tcgaagcggc aatgcatatg aagtcgaact
    35041 gtggatacca acacgtaaag gccagcggct gtttctgttt cgcaataaaa taaatttgtc
    35101 cacagcggca gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa
    35161 gagcgccgcg ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg
    35221 ccgaatcgta acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat
    35281 aaagttgggg ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg
    35341 catttgtttg gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa
    35401 catgaccagg tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc
    35461 tcacaaagcg cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt
    35521 cgtattggtt taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa
    35581 catggttcgg acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag
    35641 gaaggcggac gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa
    35701 tatctctggc tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttattcac
    35761 tatcaaccga aaatcacctg gcgtggcgaa gtgcgcagtc tggaagcact agtacgttgg
    35821 cagtcacctg aacgtgggtt gattccaccg ttggacttca tttcctacgc cgaagagtca
    35881 gggctaattg tgcctttagg ccgttgggtg attctcgatg tcgtacgcca ggtggcaaag
    35941 tggcgggata aaggcataaa cctgcgagtg gcggtaaata tttctgcacg tcagctcgcc
    36001 gatcaaacca ttttcaccgc cctgaaacag gttctccagg aactcaattt tgaatactgc
    36061 cctatagatg ttgaactgac agagagttgt ctgattgaga atgatgaact ggcactgtct
    36121 gttattcaac aatttagcca actaggtgcg caagtgcatc tggatgattt tggtaccggc
    36181 tactcttcac tttcgcaact ggcgcgcttt ccgatcgatg ccatcaaact tgaccaggtt
    36241 tttgttcgag atattcacaa acaacctgtc tcgcagtcac tggtccgggc gatcgtcgct
    36301 gtggcccagg cattgaatct tcaggtgatc gccgaaggtg tagagagtgc aaaggaagat
    36361 gcttttttaa ccaagaacgg gatcaatgag cggcaaggat ttttgtttgc caaaccgatg
    36421 cccgccgtcg ccttcgaacg ctggtataaa cgctatctga agcgcgcata agtagatgaa
    36481 tatatatact tagcgctata tcccgtcatt atatatcgta ttcctggatg ataattagcc
    36541 gttgctgtta aacacttctc ttttagcggc aatttttgca ttattcattt tccacttttg
    36601 ctataaggat gtccttgatg tctgaatttg atgcccagcg cgttgccgaa cgtattgata
    36661 ttgtgttaga tatccttgtg gcaggcgatt accactctgc gatccataat ctcgagatac
    36721 ttaaagcaga actcttgcgc caggttgctg aatctacgcc agacatcccg aaggcaccgt
    36781 gggagattta acaatcgtgc cttcaggttt tcctatttga ggatgcccgg cgcgtcagta
    36841 tttttttatt tagtattata acgttataag aattacagcg atgaattccc gacaacaaac
    36901 tattctacag atggtcattg accagggtca ggttagcgta accgatctgg caaaagccac
    36961 tggagtttct gaagttacca ttcgccagga tctcaacacc ctcgaaaaac tgagttacct
    37021 ccgccgtgca catggctttg cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat
    37081 gagcaactat acgctgaagc gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg
    37141 cgaaaccatc tttatcgaaa atggcagcag caatgccctg ctcgcccgga ctctggggga
    37201 gcagaagaaa aatgtcacta tcatcacggt cagcagctac atcgcgcatt tgctgaaaga
    37261 cgcgccttgt gaagtcattt tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt
    37321 tggcccgttg acacgccagt gcatccaaca ggtgcatttc agcaaagcat ttattggtat
    37381 tgatggctgg caacctgaaa ctggattcac cggtcgcgat atgatgcgta ccgatgtggt
    37441 caatgccgtg ctggaaaaag agtgcgaagc gatagtcctg actgacagct cgaaatttgg
    37501 tgctgtacat tcatactcca tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc
    37561 gaaaatacgc gccagcgatc tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt
    37621 tgacatttaa ccatttccct tctctacgga tgatttgcag tttggcaaat catccgctct
    37681 aagatgattc ctggttgata attaagacta tttacctgtt attaacacgc ttaagatata
    37741 aaattattat cagcgatata acaggaagtc attatcacct gcgtgatata accctgcgcg
    37801 cgagcagatt tcacggaata atttcaccag gcttattctt agctattata gttatagaga
    37861 gcttacttcc gtgaatcata aattcaggag agagtattat gtttgtaacg agcaaaaaaa
    37921 tgaccgcggc tgttctggca attactttgg caatgtctct gagtgcctgt tctaactggt
    37981 ctaaacggga ccgcaacacc gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac
    38041 tgaccgatgg cagtacgttg ggtacattag gtggtgcagc tgtcggtggt gttattggtc
    38101 accaggtcgg taaataatcg taaataaatg tcgattatcg actcgcaatt ataatacgct
    38161 tgattctgtt cagtacgaaa taactgaata atacgcacag taaaatattc gaaagccacg
    38221 gatatatccg tggcttttta tgttaaccgc ctgcgagttt taccttcatc cctttcgctt
    38281 caagtagtga ttttaataaa tcacgcttat cgccctggat ttcaataact ccatctttaa
    38341 ctgctccgcc gcagccgcat tttttcttca gttccgctgc aagttttgtc agttcggcat
    38401 catcgagatc gacaccggta atcaggcaaa cgcccttacc tttacgtcca ctggtctgac
    38461 gctgaatacg caccacaccg tcgcctttag ggcgtacagg ggccgctttg ggctcatcaa
    38521 tacgtccggt ttccgttgag tagaccagac ggctgttgga atcactcatc atgcactccg
    38581 ctgtaaagag gcgttgatcg ctttcagcgt ctgcgctgga tctaccgatt gcgttaccgg
    38641 gcgaccaatc accatataat caacaccagc cgacaacgcc tgttctggcg tcataatgcg
    38701 gcgctggtca ccagcttcac tcccctgcgg acgaatgccc ggcgtaacca gtttgaactc
    38761 ctgaccgaat acctgtttaa agcgcacagc ttcctgagca gaacacacca caccatcaag
    38821 gccacatttt tgcgtcagtg ccgccagacg ttctgcataa tctgcaggtg acagtgtcat
    38881 gccaagatcg accaggtcgc tggcttccat gctggtcaac actgtcacag caatcaaaag
    38941 cggtgcatct ttgccaaacg gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc
    39001 accagaggca tgaacattca ccatccacac gcctaagtca gctgcagcag cgacagcgtg
    39061 cgctgcagtg ttaggaatat cgtggaattt caggtcaaga aagatatcaa aaccacgctg
    39121 ttgaagttcg cgcacaaact gtggcccaaa caatgtaaac atctctttgc cgaccttcag
    39181 acgacaatcg cgtgggtcga tcttgtcgac aaaggacagc gcgtcatcac gattatgata
    39241 atcaagggca acaaccacag gagaattcgt aacagcgcgg gaagaagatg aagcagttaa
    39301 cgtcatgacc agaccttctt gatgatgggc accggaagtg gcgcgaaaca ggtaaacggc
    39361 gagcattcta cccgtggaaa taaaaaatta acagttgcga tttttcccct ggcaggcttt
    39421 ttctgctccg tcgttgccgg agcacaatgg aacatcaata actagtatgt tgtaactaaa
    39481 gtcggatttt tttaaaaatt acaggccatc aagaccgcga atcggtttaa tggttgacca
    39541 ggcccgacaa gacggacaat gccagtagag ggtgtatgcg gtaaaaccac atttctggca
    39601 gcgataacga ggcttactac gtaccttttc gccaaccatg tcacgcagca ccatcaggct
    39661 ctctttggca cgcccttctt ccgcttcatt taagtggtaa tccattaact tatggaacac
    39721 acgcatggtc ggatgacgct gaagctggcg cgtaatatag acctgtgcgg cctcactacc
    39781 gtcgcgcgct tcgatgatat cagcaagcat caattcagca tcggcaccgg tgttctcttc
    39841 caccgcgcgt tgcaggaatt ctgcccattc ggcagtttta cccaactgct gatagcaggt
    39901 ttgcaacatt tccagcgttt cgctgaccag ttctctgtcc tgggatatca cgcgttgcaa
    39961 actttcgacg gctttggcgt attctccttt cgccataaac acgcgtccca tcatgatgga
    40021 tacgcgggcg ctgtttttat ctgccgccgc cccttttttt agcaaggtca tggcacgatc
    40081 gagatcgtcg ctggccatat gctgcagggc taactcacag tagaaatggg caatttcgac
    40141 gcgctgttta tctttaccca gcttcaccag gcgttcggca acatcaattg ctttctgcca
    40201 ctcgctggta gcctggtaga tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt
    40261 ttcatcggtc agctgattga acatgtcttc cgcgcggtcg tataacccgg cggccatgta
    40321 atcacgcccc agttgttgaa tcgccaacag acgctgttca taggtcagtg aggcgctttc
    40381 cattagggtc tgatggatgc gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt
    40441 tccgagcgta aggtgggctt caacggtgcc tgtatcctct ttaagcatat cgagaaacag
    40501 gtctaccgct ttatcctgtt gattactaag caggaagtta acccccgcta cgtaatcacg
    40561 cgacaagcgg ttggcttcat cttgcttgtt ttgttgcgca cttctgcggc ccatatacca
    40621 gccataggca gcggctacag gcaaaagcag aaacaacaac tccagcatag aaagttattc
    40681 cttcgccgct gacgagtgcg gcactacagc cacgtcagtc gcgggtgaaa gctggttttc
    40741 cagtcgcttt attttacgtt cagcgcgcgc cagggaaaca cgaactcgca gccagaacag
    40801 gccacaaatc aaccaaccga tagcaacccc cgcagcaaac aataccgcca gcaatgtgga
    40861 aatacggtac tccccttgcg ctaacagata attaaacgtc acctgttgat cgttctgcgc
    40921 acccaacgtg accgaaatca cgaagatcgc taacaccagt aagaaaatga gtaaatattt
    40981 cacattactt cccgttatgt ggttaaagcg aataaattat ggtcaactta ccgcaatagc
    41041 cctttaaagt accattttcg cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat
    41101 ttaagggcta aattcggaaa atcaaccagc gttaactttc ttgttctcgt tgcgctattt
    41161 cgcgattttc ttccgcaggt ggtgttaatg gcccacaaat tcgttgcgca agccacgttg
    41221 ccaccgccac cagcgcccac gaaatcaacg tagctactac cagatcgcgt ggccaatgca
    41281 tcccgagcag caggcggctt cccatgactc ccgttgccca gaccagcaag atagcaatgg
    41341 ttaacgttcg ccgacgcggc cacagcaaac caacggccag cagtgcccaa ctggcagcaa
    41401 acatcgtgtg accggaagga aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat
    41461 attgtgggat atttttctct tcagccaact gttctttcac tagatttccg cgttctgctc
    41521 gctttaaagt gtagaactca tcaaccggaa tatgatgtgt tttttccagc cagataacaa
    41581 aaggtcgtgg ttcctggact ttgtctttga tccaggattt aacgccttgt cccacaatga
    41641 ttacggccgc cagaatggca aataatacaa aggcagcctt aatgcgaaaa cgcagacacc
    41701 agagaaacca gccgaataaa atcaaatgtg taatgacgcc ccagggctgg gtgacagttt
    41761 cagtaaccca aaaagccgct tttagtagcc aactttgttc tccaggttgc caacgccagc
    41821 cagaaatcca tacggctact ggcatgacaa gcaatagtgc agctcccact gcggtacgtc
    41881 tggcaatcga acgcatggcc tctccttttt gataagtccc acaatcataa ctgaaaacgc
    41941 cagttccagg aaaaattgac agatttgtgc cattccgtga acgatcgacg cgtcgtgatt
    42001 aggtgaaccc cttctcgtta tggcaaaata agccaataca gaaccagcat tatctggaga
    42061 atttcatgca gcttaaacgt gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc
    42121 tgatggtggg atttgaagaa ctggcaaccg gacacgatca tgtcgcgcta gtctatggcg
    42181 atatttccgg gcataccccg gtacttgcgc gcgtccattc cgaatgtctg accggtgacg
    42241 ccctgttcag cttgcgctgc gattgtggct tccagctcga agcggcattg acgcaaattg
    42301 ccgaggaagg ccgtggtatt ttgctgtatc accgtcagga aggtcgtaac attggtctgc
    42361 tgaataaaat ccgcgcttac gcactgcagg atcaaggtta cgataccgta gaggctaacc
    42421 atcagttagg cttcgccgct gatgagcgcg acttcactct ttgcgctgat atgttcaaac
    42481 tccttggcgt caatgaagtc cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga
    42541 ccgaagcagg gattaatatt gttgaacgcg taccattgat tgtaggtcgt aaccccaata
    42601 acgaacatta tctcaatacc aaagccgaga aaatgggtca tttgctgaac aaataaccct
    42661 ctcgcattgt ggaattaatt tgcttgccgg aagcaaaata accggcaagc aaatatttgt
    42721 tacttcagca tattacgaat gacataatgc aaaatgccgt cgttctggta gtaggtcaac
    42781 tccgtcgcgg tgtcgatacg acaacggcag ggtacgacct cctggctacc atccgcgcgc
    42841 gtaagcgtca ccggaaccgt cgcaccgggt tgtagatttt gcagatcgcc aatatcaatc
    42901 ttctcttccc cggttagtcc taacgtttta cgcgttacgc cttgcggaaa ttccagcggc
    42961 aggatgccca tgccaattaa attcgaacgg tgaattcgtt caaacgattc ggcaatcacc
    43021 acacgaattc caagcagacg aggacctttt gccgcccagt cacggcttga gcctgaacca
    43081 tactctttcc cggcaatcac cgccagcggc gtttgttcct gcttatagcg catcgcagca
    43141 tcataaatag agactacgtc gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca
    43201 ggcaccattt cattacggat gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg
    43261 ttaccacgcc gcgaaccgta ggagttaaag tcttttcgct caacaccccg accttgtaga
    43321 tatcgacccg ctgggctgtc gggcttaata ctgcccgccg gagagatatg gtcagtggtg
    43381 actgaatccc ccagcattgc gaggatccgc gcaccgtgaa tatcttccac tggtgctggt
    43441 gttgcctgca tttcatcaaa gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa
    43501 ccgtaggtat cggatcgtgt gacgttaatt tccttccact ctgctgtgcc ttcaaaaact
    43561 tctgcgtact ctttgcggaa catttctgtg gagacttgtt ctaccgcacg ggcaatttct
    43621 tgtgccgatg gccagatatc tttcagataa accggatcgc ctttacgatc atgaccaata
    43681 ggctcagaag ccaggttgat attcatattt cccgccagcg cataggcaac caccagcggc
    43741 ggcgaggcca gccagttggt tttaaccagc ggatggatac ggccttcaaa gttacggttg
    43801 ccggacagca ctgcaccgac ggttaaatcg ccttttttga ttgccgtttc gataggatcg
    43861 ggcagcggcc cagagttacc aatacaggta gtacaaccgt atcccacaag gttaaacccc
    43921 agttcgtcga gaaacggtgt cagtttcgct tttgccagat agtcagaaac aactttcgag
    43981 cctggtgcca gcgacgcttt gacccacggt tgccgcttga ggccaagaat tacggctttt
    44041 ttcgccagca agcctgcagc catcagcaca cttgggttag aggtgttggt gcacgaggtt
    44101 atcgcagcaa tgaccacagc gccatcaggt aactgatact gatgtccgtt cataacataa
    44161 tcgaccggct ggcgatcttt atgcgtggca ttcacttcca gttcgttact ggcggcaaat
    44221 acttttggta catcgggcag tgcaacgcga tcctgtgggc gttttggccc tgccaggctc
    44281 gcttcaacgt cattcatatc cagttctaac gtactggtaa aaatcggttc atcgcccggg
    44341 ttacgccaca tgccctgcgc tttggcatat ttttcgacca actcaacctg atcttcgctg
    44401 cgcccgctta aacgcatgta atcgagggtt acagcatcga ttgggaagaa gccacaggtg
    44461 gcaccatatt ctggcgacat attggcaatg gtggcgcgat ccgccagcgg tagtgaatcc
    44521 agaccatcac cataaaattc gacgaatttc tccaccacgc catgtttgcg cagcatttgg
    44581 gtaacagtga gaaccaggtc tgtggcggta ataccttcac gtaatttacc ggtaagttta
    44641 aagcccacta cgtccgggat aagcatggaa accggctggc ctaacattgc ggcttctgct
    44701 tcgatcccac ccacgcccca ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag
    44761 tcagtaccaa cgagtgtatc cggataagca atccattcac cgtcctgcaa ttcactccac
    44821 actgctttgc cgagatattc gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg
    44881 acgctaaacc gactgaacgc ttgctttccc catttcagga acacataacg ttcgtggttg
    44941 cgctccattt ccaggcgtac gttttcttca aatgcctcat catcaccaaa acgatcgacg
    45001 gtcaccgagt ggtcaatgac caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg
    45061 ccgccgaggc gtttaaccgc ttcgcgcatt gccgccagat caacaacggc aggtacgccg
    45121 gtaaagtcct gcatcaacac ccttgccggg cggtaggcaa tttcacggtc agcatgggca
    45181 tttttcagcc atcctgccag cgcgtggata tcctcttcgg taaccgagtt accatcctgc
    45241 cagcgcagca ggttttcgag caaaactttg agtgacttgg gtagacgggt gatatcgccc
    45301 agtgatttag cagcaagcgg caggctgtag tagtggtaag ttttatcttt ggcctgcaac
    45361 gtgtccttac tggcttctcg tagggttgac gacatagctc ctccttaatg acagggttgc
    45421 gaagccctga ttctcttcag ggtgaatatt aaagataaca caaacatcgc gtaacgattt
    45481 gataacaacc caaattgata aaagaggaga aaccttgtgg caaagcaaat gacaaccccg
    45541 ccgcagcggg gtcaaggatc agtgagtaat gatccagagg agttcggccc agaagagcat
    45601 cgacagagaa aacgcgccca tccaggacca atatttcata tttgtgttga tcataggcta
    45661 cacccttaac ggttattatt ttgtgttgag ataactcaag ctgttattgc tgcggtgtat
    45721 taaaagccct ttaattcatt aaaaatgggt aataagaatg atgataatta ttgtttcggc
    45781 tgatcatctt cagcaaacag ttctataaag gcttgctgct gcgatgttag ttgccagtcg
    45841 tctggaataa cgacggaaca ttcccgtttc gtttctgttt cattgtcact atgctggcat
    45901 aatggtgacg actccagttg ctcctgaccg accatagtta tccccacact ttccaaatca
    45961 gcagtgcgac aaccacccag aaaagtgcag aaccaagaaa aaccgccagc caggctttac
    46021 gttttaattc aggatcgcgg cggggttcct gatttccaga aggcattgct aacctcatat
    46081 aatcgccatc acttatcagc aagacgttga acgattggtg taattaatca tacgatgaga
    46141 caaatcctaa agaaacttta gtcaaa
//
LOCUS       DAZPEP010000015        45644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZPEP010000015 DAZPEP010000000
VERSION     DAZPEP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.015"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(44..1903)
                     /gene="gss"
                     /locus_tag="ACHUT9_000850"
     CDS             complement(44..1903)
                     /gene="gss"
                     /locus_tag="ACHUT9_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFR1347399.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2108..2974
                     /gene="yghU"
                     /locus_tag="ACHUT9_000851"
     CDS             2108..2974
                     /gene="yghU"
                     /locus_tag="ACHUT9_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFR1347400.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3003..3084)
                     /locus_tag="ACHUT9_000852"
                     /pseudo
     CDS             complement(3003..3084)
                     /locus_tag="ACHUT9_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3097..3345)
                     /gene="hybG"
                     /locus_tag="ACHUT9_000853"
     CDS             complement(3097..3345)
                     /gene="hybG"
                     /locus_tag="ACHUT9_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFR1347401.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3358..3699)
                     /gene="hypA"
                     /locus_tag="ACHUT9_000854"
     CDS             complement(3358..3699)
                     /gene="hypA"
                     /locus_tag="ACHUT9_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1347402.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3692..4180)
                     /gene="hybE"
                     /locus_tag="ACHUT9_000855"
     CDS             complement(3692..4180)
                     /gene="hybE"
                     /locus_tag="ACHUT9_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFR1347403.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4173..4667)
                     /locus_tag="ACHUT9_000856"
     CDS             complement(4173..4667)
                     /locus_tag="ACHUT9_000856"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFR1347404.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4667..6370)
                     /gene="hybC"
                     /locus_tag="ACHUT9_000857"
     CDS             complement(4667..6370)
                     /gene="hybC"
                     /locus_tag="ACHUT9_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFR1347405.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYGGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6367..7545)
                     /gene="hybB"
                     /locus_tag="ACHUT9_000858"
     CDS             complement(6367..7545)
                     /gene="hybB"
                     /locus_tag="ACHUT9_000858"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFR1347406.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7535..8521)
                     /gene="hybA"
                     /locus_tag="ACHUT9_000859"
     CDS             complement(7535..8521)
                     /gene="hybA"
                     /locus_tag="ACHUT9_000859"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFR1347407.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8524..9642)
                     /gene="hybO"
                     /locus_tag="ACHUT9_000860"
     CDS             complement(8524..9642)
                     /gene="hybO"
                     /locus_tag="ACHUT9_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFR1347408.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9831..10118)
                     /gene="yghW"
                     /locus_tag="ACHUT9_000861"
     CDS             complement(9831..10118)
                     /gene="yghW"
                     /locus_tag="ACHUT9_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFR1347409.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10237..11124)
                     /gene="yghX"
                     /locus_tag="ACHUT9_000862"
     CDS             complement(10237..11124)
                     /gene="yghX"
                     /locus_tag="ACHUT9_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFR1347410.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11281..12321
                     /gene="gpr"
                     /locus_tag="ACHUT9_000863"
     CDS             11281..12321
                     /gene="gpr"
                     /locus_tag="ACHUT9_000863"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFR1347411.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12361..12855)
                     /locus_tag="ACHUT9_000864"
     CDS             complement(12361..12855)
                     /locus_tag="ACHUT9_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFR1347412.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13046..13930
                     /gene="yghA"
                     /locus_tag="ACHUT9_000865"
     CDS             13046..13930
                     /gene="yghA"
                     /locus_tag="ACHUT9_000865"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1347413.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14090..14515)
                     /gene="exbD"
                     /locus_tag="ACHUT9_000866"
     CDS             complement(14090..14515)
                     /gene="exbD"
                     /locus_tag="ACHUT9_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFR1347414.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14522..15256)
                     /gene="exbB"
                     /locus_tag="ACHUT9_000867"
     CDS             complement(14522..15256)
                     /gene="exbB"
                     /locus_tag="ACHUT9_000867"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1347415.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15508..16695
                     /gene="metC"
                     /locus_tag="ACHUT9_000868"
     CDS             15508..16695
                     /gene="metC"
                     /locus_tag="ACHUT9_000868"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFR1347416.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16835..17494
                     /gene="yghB"
                     /locus_tag="ACHUT9_000869"
     CDS             16835..17494
                     /gene="yghB"
                     /locus_tag="ACHUT9_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFR1347417.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17534..18433)
                     /gene="yqhC"
                     /locus_tag="ACHUT9_000870"
     CDS             complement(17534..18433)
                     /gene="yqhC"
                     /locus_tag="ACHUT9_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFR1347418.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESALQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18627..19790
                     /gene="yqhD"
                     /locus_tag="ACHUT9_000871"
     CDS             18627..19790
                     /gene="yqhD"
                     /locus_tag="ACHUT9_000871"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFR1347419.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19895..20722
                     /gene="dkgA"
                     /locus_tag="ACHUT9_000872"
     CDS             19895..20722
                     /gene="dkgA"
                     /locus_tag="ACHUT9_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFR1347420.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20922..21848
                     /locus_tag="ACHUT9_000873"
     CDS             20922..21848
                     /locus_tag="ACHUT9_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFR1347421.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21899..22156
                     /gene="yqhH"
                     /locus_tag="ACHUT9_000874"
     CDS             21899..22156
                     /gene="yqhH"
                     /locus_tag="ACHUT9_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFR1347422.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22199..24418)
                     /locus_tag="ACHUT9_000875"
     CDS             complement(22199..24418)
                     /locus_tag="ACHUT9_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFR1347423.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24529..25941)
                     /gene="ftsP"
                     /locus_tag="ACHUT9_000876"
     CDS             complement(24529..25941)
                     /gene="ftsP"
                     /locus_tag="ACHUT9_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFR1347424.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26016..26753)
                     /gene="plsC"
                     /locus_tag="ACHUT9_000877"
                     /gene_synonym="parF"
     CDS             complement(26016..26753)
                     /gene="plsC"
                     /locus_tag="ACHUT9_000877"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFR1347425.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(26986..29244)
                     /gene="parC"
                     /locus_tag="ACHUT9_000878"
     CDS             complement(26986..29244)
                     /gene="parC"
                     /locus_tag="ACHUT9_000878"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFR1347426.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29790..30272)
                     /locus_tag="ACHUT9_000879"
     CDS             complement(29790..30272)
                     /locus_tag="ACHUT9_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFR1347427.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30325..30717)
                     /gene="ygiW"
                     /locus_tag="ACHUT9_000880"
     CDS             complement(30325..30717)
                     /gene="ygiW"
                     /locus_tag="ACHUT9_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFR1347428.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30869..31528
                     /gene="qseB"
                     /locus_tag="ACHUT9_000881"
     CDS             30869..31528
                     /gene="qseB"
                     /locus_tag="ACHUT9_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFR1347429.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31525..32874
                     /gene="qseC"
                     /locus_tag="ACHUT9_000882"
     CDS             31525..32874
                     /gene="qseC"
                     /locus_tag="ACHUT9_000882"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFR1347430.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            32984..33565
                     /gene="mdaB"
                     /locus_tag="ACHUT9_000883"
     CDS             32984..33565
                     /gene="mdaB"
                     /locus_tag="ACHUT9_000883"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFR1347431.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33596..33910
                     /locus_tag="ACHUT9_000884"
     CDS             33596..33910
                     /locus_tag="ACHUT9_000884"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFR1347432.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(33958..35850)
                     /gene="parE"
                     /locus_tag="ACHUT9_000885"
     CDS             complement(33958..35850)
                     /gene="parE"
                     /locus_tag="ACHUT9_000885"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFR1347433.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35879..36460)
                     /gene="yqiA"
                     /locus_tag="ACHUT9_000886"
     CDS             complement(35879..36460)
                     /gene="yqiA"
                     /locus_tag="ACHUT9_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFR1347434.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36460..37287)
                     /gene="cpdA"
                     /locus_tag="ACHUT9_000887"
     CDS             complement(36460..37287)
                     /gene="cpdA"
                     /locus_tag="ACHUT9_000887"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFR1347435.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37312..37734)
                     /locus_tag="ACHUT9_000888"
     CDS             complement(37312..37734)
                     /locus_tag="ACHUT9_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFR1347436.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37735..38364)
                     /gene="nudF"
                     /locus_tag="ACHUT9_000889"
     CDS             complement(37735..38364)
                     /gene="nudF"
                     /locus_tag="ACHUT9_000889"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFR1347437.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38569..40050
                     /gene="tolC"
                     /locus_tag="ACHUT9_000890"
     CDS             38569..40050
                     /gene="tolC"
                     /locus_tag="ACHUT9_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFR1347438.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40198..40869
                     /locus_tag="ACHUT9_000891"
     CDS             40198..40869
                     /locus_tag="ACHUT9_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFR1347439.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40875..42035
                     /locus_tag="ACHUT9_000892"
     CDS             40875..42035
                     /locus_tag="ACHUT9_000892"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1347440.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42073..42861)
                     /gene="ygiD"
                     /locus_tag="ACHUT9_000893"
     CDS             complement(42073..42861)
                     /gene="ygiD"
                     /locus_tag="ACHUT9_000893"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFR1347441.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43004..43777
                     /gene="zupT"
                     /locus_tag="ACHUT9_000894"
     CDS             43004..43777
                     /gene="zupT"
                     /locus_tag="ACHUT9_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFR1347442.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43835..44005)
                     /gene="yqiD"
                     /locus_tag="ACHUT9_000895"
     CDS             complement(43835..44005)
                     /gene="yqiD"
                     /locus_tag="ACHUT9_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFR1347443.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44266..44919)
                     /gene="ribB"
                     /locus_tag="ACHUT9_000896"
     CDS             complement(44266..44919)
                     /gene="ribB"
                     /locus_tag="ACHUT9_000896"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFR1347444.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45023..45171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45293..45583
                     /gene="ubiK"
                     /locus_tag="ACHUT9_000897"
     CDS             45293..45583
                     /gene="ubiK"
                     /locus_tag="ACHUT9_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFR1347445.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11260 a        12249 c        11464 g        10671 t
ORIGIN      
        1 ctgattattc ttcataatca gtacggggtt gctacaacat gtattacttt ttcaccacaa
       61 ttaacggttc aatatcactc tcttttttga tgaccagtga ttcatcaccg cgcaaacacg
      121 tcccaccgta gttgccgccc acggtgaagg tacatacctg aatgtattta ccgtccactt
      181 tcggcaaaca ccacagttgc tgatagatgt ttttctgctc ggcaaattta ccgctggttt
      241 tgtccagcac ctcttcatga tggctgacca ggtcgatatt gctaccacag cgaccggcaa
      301 tcggtttcac tgcgtaacct gttttcacca attcatcatt aacagtgaaa tcggtatcca
      361 gcaggtaacg atggtgcggg aacagcgacc agaggatcgg cagaatcgct ttgttgccgg
      421 ggatcaccgt ccacagcggc tcaaagacta gcacttccgg gcgcagcaat acgtcgataa
      481 gacgcacttc gttttgcgga tgaccggtac ggattggcac cgcagcaaac tcacggtcgc
      541 taacttcacg aatctgatca aacgcggttt cccacgccca ggttttccac acgcagttaa
      601 ccagtcgccc ttccccatca atcagttgcc cggcagcatc ccagcccagt tcatccaatc
      661 cacgcaagat acgcgtttca aagcccgcct ggtgcagcgc ctgctccata aactgcgcgt
      721 gatagttttc ctcgatatct ttgtcctgca tgatatggac aaacggacgt gcacgactgt
      781 gtttccaggc accagccaat tcgttaatca gcccttccgc cggattgaag ccgttgcctt
      841 tatagccctg ctccgcccaa cgttcgagga tcaagcccgc ttcggtatga caggaggcgg
      901 agtcggcgtt gtactcgtaa accttcaggc cacgctcatc catgcagaaa tccatacgac
      961 cagtgatcat atggtgacgg cgacgctgcc aggagagacg caaacgtggc cagaggattt
     1021 tcgggatgtc gaacagcgcc agcaggttgt catctttcag caccttgtcg gttgcgtgaa
     1081 gatacatcag gtgcagctcg ttggtggctt taattagctc ctgctcggca ctctcggtaa
     1141 tggtgtagta atgataagga tcctgattga tcacctgacc gttggcctgc acataggcgt
     1201 tttgcagcgg atctttttca tccagccatt taccgtcaaa ctggcctttg ttttccaggc
     1261 gcgctccgct gattttcagc agctcgcctg caatttccgg ctgcggtaag ctgtattcag
     1321 tatcttccgt ctggatcatc cagcccagaa tggtggtgtc atcaaaagtg tctttcaggg
     1381 tatagcagcc gttttcgacc accatctcca gctcacgcgt ccactgttgc ccttgcggca
     1441 acggggaatg aatcacgttc tgttccgcaa tacggacttt gttgccatgc aattgggtaa
     1501 tgatggcgac atggccagtg tctttaaatt caccgccttt atcccagata agaagcgcac
     1561 ccgcgaccgg cgcacgcggc gagccgttag gaaatgcctg caatggcagg atgttgtcat
     1621 taaccacttc tcgcaggaag cgcagcgaga aaatctccca cgccataccc acgtcagtaa
     1681 agaccacacc gtaattcaga aagagaaaac ggcgagcaaa ttcaacgcat tgccacttgt
     1741 ggcccatata ttcgtcgtcg atatagctac ggaatacggc gtcatcttcg tattcctgcg
     1801 gatcgagaga actgtaatct gaagagtaga ttgctacccc acctggggcg tagcccaata
     1861 atgtcccgaa cggggcatcc tggctggtcg ttcctttgct catcacttta cctttaacaa
     1921 tacaacctaa gcagttggcg taattgttgt gctctgatta cctgctcagc gacattaacc
     1981 ggacagaggt cagactaatc atacacctca tcgcagcggc tggcgcaaag ggttaaaaaa
     2041 ttcacattct gtacagcaag tgacctgcta cactgcttca acactaccac tcagaaggca
     2101 actcactatg acagacaata cttatcagcc cgcgaaagtc tggacgtggg ataaatccgc
     2161 tggcggcgcg ttcgccaata tcaatcgccc ggtttctggt ccgacgcatg aaaaaacgct
     2221 gcccgttggc aaacacccat tgcaacttta ttcgctggga acgccgaacg gtcagaaagt
     2281 aacgattatg cttgaggagc tgctggcgct gggcgttact ggtgcagagt acgacgcctg
     2341 gctgattcgt attggcgatg gcgatcaatt ctccagcggc tttgtcgaag tgaacccaaa
     2401 ctcgaagatc ccggcgctgc gcgatcatac gcataatccg ccgatccgcg tgtttgaatc
     2461 tggttcgatc ctgctttatc tggcggagaa atttggctac ttcctgccgc aggatttggc
     2521 aaagcgtact gaaacgatga actggctgtt ctggttacag ggcgcggcac cgttcctcgg
     2581 cggtggtttt ggtcactttt accattacgc gccgatgaaa attgagtacg ccatcaaccg
     2641 ctttaccatg gaagccaaac gtctgctcga cgtgctggat aagcaactgg cgcagcataa
     2701 gtttgttgcg ggcgatgagt acaccattgc ggatatggcg atttggccgt ggtttggcaa
     2761 tgtggtgtta ggcggcgtgt atgatgccgc agagtttctt gatgcgggca gttataagca
     2821 tgtacaacgc tgggcgaaag aagtaggcga acgtccggcg gtgaaacgtg ggcgtattgt
     2881 taaccgcacc aacggaccgc tgaatgagca gttgcatgag cgccatgacg ccagtgattt
     2941 cgagacgaat acggaagata agcgtcaggg gtaagggttg gtgttcgtcg cagcaagcca
     3001 tccaggccgg ataaggcgtt cacgccgcat ccggcaatcg tgcacaatgc ctgatgcgac
     3061 gctgccgcgt cttatcaggc ctacgaaagc taatcatcac gcgctggtaa tgtcgtaatc
     3121 catttggcgc agtgcgtcta atgtggcttt ggcttcatct tcgtcgatga tgctcatggc
     3181 aaatccgacg tgtaccagca cccactggcc cagtagatcg gcagggttac cttcacaaat
     3241 cagggcgata ttcacgtcgc gcttgatacc acatacttca acctgcgcaa gctggtgaat
     3301 atcttcaccg acagccagca cctggcctgg aacgccaata cacattatta actccggtta
     3361 ttcaacttca atacttttga cgatcagcga atcgccggta tcgacacgca accgctcgcc
     3421 gtgacagagc ggacactgcg catcgtgctg atgaatctcc accacctggc tgcaatccca
     3481 gcaccaagcc tgggcgggtt tatagacgat atgtaaatcg cacccttgcg ccaccgttcc
     3541 gtggcagaca atttcaaaac taaaacggac ggcgctctcc tcaacgcagg agagcgcgcc
     3601 aatttccagc cacacggcgg tgacgcgctt aacatcgtgc tgctccgcct gccgttggat
     3661 aatttcaacg gcgctctggc aaagagacaa ctcatgcatt ttcgccactc ctgcgaccaa
     3721 acagcagggc gcgacgccct gcgtgtggta catccggatt cgtgactggc agcgaaagga
     3781 tcattcgtgc gcagtcatcc gtcaggcgtt ggccctcttc aatcgacatg ctgtgcgaaa
     3841 gcggcgacat cagcgaacag gagagatatt gcgaaacacc gtccagttcg ccaacggtaa
     3901 aggtcatagt gccatacggc agttgcagac caattttttc actcactttg cgcagcggcc
     3961 agagttgatc cgggccgggg aagataactg cactcagcat ccacggggtg atcacacacc
     4021 ccgtccactg accttcgaac agcgtaaaat cagaaacata caccggcatt gaaggatgca
     4081 gaaaagagag atcgtgcatc gaacgccggg caatttcttc aaacgctgcc tgtacttgcg
     4141 ccttcgggga ggtctggaaa cctgctatct cttcagtcat gaatcgcctc ccgtgggatg
     4201 gcttccacgc ctgattcgcg cagcgcagcc agaacctgct caagcgcagg ttcaatcatt
     4261 gcttcaaccg tcggcgttaa gccgatgtgt ggctccagcg attccgggat cacgccgacc
     4321 agggtcagct ttttcggaaa ctcaccggtg aagcgcaggg ccgacaatac gtcggccagg
     4381 ccaagctgat gcggagagat tttgttggta aacaacgccg gaacttcttc atcccgcagg
     4441 atcatcatcg ttcccggcgt gttctttttc gacacaatgg catccgcaat aatcagatga
     4501 tcgcgatttg ccatgtcgcc aagcagctcc attcccgccg tgccgccatc gaggatctca
     4561 acataatccg gcagaatgta tcgttgctct aacgcttcga caatccgcac accgatggct
     4621 tcatcggtca gcaaaatatt gccgacccct aagactaaaa tacgcattac agaaccttca
     4681 ctgaaaccac ttcgttgccg tcggcatcca ctacgtgtac cgcacaggcc atgcacgggt
     4741 cgaaggagtg aatggtacgc accacttcca gcggtttatt cggatcagca accggtgtac
     4801 ccaccagcga ctgctcgtaa ggaccgacgt catcattgaa gttacgcgga ccagagttcc
     4861 aggttgatgg aacaactgcc tggtagttac tgataatacc gtctttaata accatccagt
     4921 gagagagcat accgcgcggc gcttcgagga agccaacacc tttgaactca cccgttgccg
     4981 gaatgttcgg tttcacaaag gtggtgtgat cgcctttgcc gatattggtg atcagtgcac
     5041 tgtattgatt ttgcaggata tcctgcaatt cgcagcagtg aacggtacga ccaataatac
     5101 ggcccagcgt ggagtgcagc tgcgccactt ccagcgtgtt gccagtcagt ttctgataaa
     5161 tcgcaacgat ttcattcagt ttgttctggg tagattcgcg acctgccgcc agtttcacca
     5221 gcatattagc cagcggaccc acttctaccg ttttgccgta gaaagtcggt gatttcaccc
     5281 aggaatattt cccgtcgtca gaccaaccat cataagccgg aatggtggta ccttcccacg
     5341 gtgcctgcgg cgcttcgtct ttataccagg agtgcttcgc gctttcctga atgcctttga
     5401 tcaggtattc atcggaatga gaagtgatcg gacgatacga ggacagatcc gcattctcaa
     5461 tgtagccgcc cgggaacagg aagctgccgt ttttactgtc ggtcgggaat tccggcacgc
     5521 tcaggtagtt caccgcacct ttaccgcgcg tcagccattc cgggtagaac gcggcaataa
     5581 ctgcggtatc aaccttataa acctgttcaa caaagtcgct cagcttgtcg atgaaagact
     5641 tgatgtacat caggcgctca aggttcagca cgcccaaacc gtcgaggttg atcgggttcg
     5701 cgacaccacc taccgccagg ttctgaatgt gcggcgtttt accgcccagc agcgccacga
     5761 cgcggttagc gtcacgctgg cactccagcg cctgcaggta gtgcgctacc gcaatcaggt
     5821 tcacttccgg cggcagtttc atcgccgggt gacccccgta gccattggcg aaaataccca
     5881 actgaccgct ggcaaccaga tctttgatct tgttctgaac tttagtgaac tcttccggac
     5941 tgttcagatg ccaggtcgaa acgcctttca gcatttcgga ggctttggtt gggtcagctt
     6001 gcagtgcaga agtgatgtcc acccagtcca gcgccgaaag ctgatagaaa tgaacgatat
     6061 ggtcatgcgt ggtgtgcgca gccagaatga tgttacggat gtattgcgcg ttaaccggaa
     6121 cgtcgatatt cagcgcactt tctgccgcac gaacggaaga cagcgcgtga gtggtagtac
     6181 atacgccaca gatacgttgc acaatcatcc atgcatcgcg cggatcgcgg tttttcacga
     6241 tctcttccat gccgcgccac atggtaccgg aagcccatgc tttcgaaacg acgccatttt
     6301 cgatttcgca atcgatgcgt aaatgcccct caatacgggt taccggatca atagtaattc
     6361 tctggctcat gctttgctcg cctcatgacg attatgatcg ttttgtttta aaggaggaag
     6421 tatcggcagt agacgaatga gtacgatgta agcgcaaatc tcaatagcca caaaaccaat
     6481 agaaatcaac agttcttccc aggtcgggaa gtaggcgtaa ccgccgcccg ggttgaatgc
     6541 caccagcgaa taggtcagac gccaggttgc acaacctaac agtgcgctca gtgctgacag
     6601 gaacagcatg cgggaatcat tacgcagctt cgccacacgc agaacgacca gcgggaagag
     6661 catcagcagg acttcaatcc agaacatcac ggagtagaag tcaccggcaa acgctaacga
     6721 cagcttgtcg cgatagataa gctcgccaaa gcgcagcacg atgaaaatcg ccagcaacac
     6781 actgatggtg ttggtcagct tgacgaacag actcttttca tccggaccgt tgccacgcag
     6841 acccgcctgc accagcgaac cttcaaagat gacaatcgag aagcccatga tgaacgccgt
     6901 cagcagcgag aacagcggca acatttcata gctctgccac aacggatgca ccttgtagcc
     6961 cgccgagatc atcagtgacc ccattgaaga ctggtgcatg gtcggcaaca gcgcgccaag
     7021 tgcaatgatg aagaacataa ccttgttcag acgcttgaga gaaaccttcc agcccagacg
     7081 ttcaaacagt gccggagcaa actccagtgc catcacgccg atatagatgg tcatacagac
     7141 cgccgtctcg aacagtaccg agttcacgtt gaagtgaccc ggaatgtaga agtacggcag
     7201 gttccagtag cgacccacgt cgatagtgat cgacaagcca cccagtgagt aaccaaacag
     7261 actcgccaac agcgccggac gcaccagcgg atggtattgc ccacggttaa agacgtatac
     7321 cgcccacgcc agcgcccagc cgccacaggc aaagccggtg ccaatcagca ggtcaaacgc
     7381 gatccacacg ccccacggga agccgccgtt caggtcagag acagagccca gaccgaacac
     7441 cagacgcttc acaatcagga gcatacagat gacgattaac ggtccaaaaa tcatgaccgg
     7501 tttactgatg attttgccgc ccagcggttg tggatcatga ctcatgatcg tctcctccgt
     7561 cgtgatggtc gtttttggtg ttgcgacgaa ccagcacggt taagcccgcc agcacagcca
     7621 gtggtagcat catgccttta tacagggtgt gttgaatatt ttcggaacgc gcaccggtag
     7681 aaagatcgtc cagtttcggc aggtcgagat tttcataagg cacacccgtc agtaccagaa
     7741 cctgagtacc gccgccctct ttctcgccgt acagatgcgg ataatatttc ggcaccgtat
     7801 gcaggtaagt gtcgccagat ttcagcgtct gacgcggata gtggtattcg ctgccaggct
     7861 tcagcgccag acgttttttc gcctccgcca tcagctcttc acgcgtaccg aaaatcaccg
     7921 cgcccgccgg gcacacttct acgcagccag gcagaccgcc tttatcgaga cgttccacac
     7981 ctttctggtt gcacagctcg cacttatgca gcgcaccaaa cgggttgttg tagtcgtact
     8041 tcggcacgtt gtacggacag gcgaccatgc agtaacggca accggtgcac acgtctttgt
     8101 cgtaatggac aatgccggtt ttcggatctt ttttcagtgc agagaccggg cacacagaga
     8161 cacagttcgg atcgacgcag tgcatacact gtttcttaat gtacgcgtag ccgttctcct
     8221 cctggtcttt gttgacccct gtgccgctgg tccacacctg aatgatgtta ttggtatacg
     8281 gcgacagttt gtcgttgttc gaccaggtct gttccccttg cgggttacgt tcagggaagt
     8341 tgatatcctg acacttggtg acgcaagcct ggcagcctac gcacaaggtc gagtcgtaca
     8401 acatccccag cgatcccgga attggcgggc ggttttcagc agccgcatga ctgacagacg
     8461 gcagcgcgcc cgtcagcaat gccccgcagg aggctgcttt aataaaatta cgtctgttca
     8521 cggttattct ccccgtgagt cagcgttatc tttcttttgc tgacgaccca gttcacgcac
     8581 cgccatcacg ctgacaccgg caaccagccc aaccacaccg ccgagcaaac caatagcgcc
     8641 tgcagagacg ttgccgccct ctttagcgtt aacatccggt ttctgtgaac gcggagtctg
     8701 attttcgacg ttggcaagct gatggatgcc tttatggaag ccgatacctt cttcgttaca
     8761 gccatagcat gggtgaccaa tcgctaccgg ccacacgccg ccaacatcgc agaattgcag
     8821 cgttgagcag ttgccgtaag tttctggccc tttacagccg aggtggtaca ggcaccagcc
     8881 ttcacggtgg ccttcatcac cgaactcttt ggcaaaacga ccagcatcga agtgcgggcg
     8941 acgttcgcag tgttcgtgaa tcagacggcc ataggcgaag gtcggacggt ttttgtcatc
     9001 cagtttcggc ggtttgccgt aagtgatgat gtgcgcaacg gtcgcgagga agttgtgcgg
     9061 gttcggcggg cagcccggaa tattgataac ggttttgcct ggcagaactt cttgcaggct
     9121 gactgcgcca gttgggttaa ctccagctgc ggcaacaccg ccccacgcag agcaggaacc
     9181 gatagcgata atcgctgctg cgccttctgc cgctttgcgg atgtgatcca caatcggctc
     9241 accagcaacc atgcaataaa taccgttatc ttttaatggg atggaaccat ccacaaccaa
     9301 cacatactgc cctttgtact tctcaagtgc gttatgtttg ttctcttcga cctgatgacc
     9361 gaaggcggcg gaaagcactt cgtgatactc cagagagata gtctccagca cgaggttttc
     9421 taccgttgga tgcgttgcac gaagcagaga ttccgtacaa ccggtgcact cctgcgcgcc
     9481 aatccagata actggcggac gctgcgggtt agtaaccgat tcggccatct ctgcagcggc
     9541 tttgctactt aaccccatgg tggcggctaa tgctgcacaa agcttcatga aatcacgacg
     9601 gttaatgccg tgagaatgga tgagggtgtt atctccagtc atttatagtt attccgttgc
     9661 gaagacctgg catatatttt gcctcaatcg caaaatcaat aatgcgatcg atgcgccatt
     9721 taccacacat ttattatggt tatcgatatc atgatactgc ggcggggcgc taaaacgaag
     9781 ggaagaagca ttagtgtaat taattaaagc aagataatac gtatgtttga tcaattttcg
     9841 aggcgataac cggccataaa aaagcgcaac gtagaacagg aattattctc acgaaagaaa
     9901 tccattacca tctctttatc cagtccatag cggcgcgtca gaatacccgc ttcccaggcg
     9961 ctaagctggc gatctccggt cttttcgtac atccggtgtg agtagcctaa tacaaaaccg
    10021 cgtttataat cggcacagaa ttttgtcaca ttaaccgcac tgtcggcatg cgttgctttt
    10081 aatcccgcca ttaagccttt accaaaatgg ttattcatcg ttcacctcat tcgctatata
    10141 tcgccatata tagcgatatt ttaggcaaag aacgactttt gcgcacgcta ttttttcatg
    10201 cttgtttaag acgtaatcca gtaagagata aacctatcag gagagatatt tatcgaacca
    10261 tttcagtgtc ctttgccagg caagatcggc ggcagatttg tcataacggg gcgtggaatc
    10321 attatggaat ccgtgattaa cccccggata gatatacgcc tcataaacct tattattggc
    10381 tttcaacgcc gcctcgtaag caggccagcc ctcgttgatt cgggtgtcca gttccgcgaa
    10441 gtggagtagt aaaggcgctt caatcttcgc cacatcggca gtgggtgcct gacgaccata
    10501 aaacggcacc gcgcaggcca gttccggata cgcgacagcc gccgcgttcg atacgccacc
    10561 gccatagcaa aatccggtaa tacccacttt gcctgccgct tgtggatagc gttgcataaa
    10621 ctcaattgcg gcaaagaaat cattcatcag tttggttgga tcaacctgct gttgcagctc
    10681 acgaccttta tcatcatttc ccggataacc tccaacggaa cttaagccgt caggtgccag
    10741 ggcgatatac cccgccttcg ccactcgccg tgccacatct tcgatatacg gattcagtcc
    10801 acgattctca tgcaccacca ccacggctgg cgttttgccg ctcatttttg cgggtttcac
    10861 cagataaccc cgtacctcgc cgtgaccatt tggcgaagga tacatgatgt actcagcaac
    10921 aatctccggg tcggtaaact ctacctgagt cgccagcgca taatttggct tgagcaaatc
    10981 aaacaacgct aatgccgtca tcccgcccac cgcatacttc gccgcaaggt tgaggaactc
    11041 acgtttcgaa attttcccgt gagcgtaata gtcgtagtaa tccaacaact cttgtgggaa
    11101 atctttggcg gttaaacgcg gcatcgttgc actcctcagt tggtgttttt ttaagcaaag
    11161 cataagcacg tgtttttgcc cagtttttcg tcactctgtg agccagacta cgggatacgc
    11221 gctggcgaat cgctaaacta gaaacattgt ttcgaaattg aacggtggaa aggagaggtc
    11281 atggtctggt tagcgaatcc cgaacgttac gggcagatgc aataccgcta ttgcggaaaa
    11341 agcggtttac gcctgcccgc gttatcgctc ggtttatggc acaatttcgg tcacgttaac
    11401 gcgctggaat cacagcgtgc gatcctgcgt aaagcgtttg atttgggcat tacgcacttt
    11461 gatttagcca acaattacgg gccgcctcca ggaagcgcag aagagaactt tggtcgcctg
    11521 ctgcgggagg attttgccgc ttatcgcgat gaactgatta tctctaccaa ggctggctac
    11581 gatatgtggc ccggccctta cggctctggc ggttcacgta aatacctgct cgccagcctc
    11641 gaccaaagcc tgaagcgtat ggggcttgag tatgtcgata tcttttactc tcatcgcgtc
    11701 gatgaaaata cgccgatgga agaaaccgcc tctgcgctgg ctcatgcggt acaaagcggt
    11761 aaggcgctgt atgtcgggat ctcctcttac tcgccagagc ggacgcaaaa aatggtcgag
    11821 ttgctgcacg agtggaaaat tccgctttta attcatcaac cttcgtacaa tttactgaac
    11881 cgctgggtgg ataaaagcgg cctgctggat accctgcaaa ataacggcgt gggctgtatt
    11941 gcctttactc ctctggctca gggattgctg accggaaaat atctcaacgg cattccgcaa
    12001 gattcacgga tgcatcgtga agggaataaa gttcgtggtc tgacgccgaa aatgcttacc
    12061 gaagccaacc tcaacagcct gcgcttattg aatgaaatgg cacagcagcg tggacaatca
    12121 atggcgcaaa tggcgttaag ctggttgctg aaagatgatc gcgtgacgtc ggtattgatt
    12181 ggtgccagcc gcgcggagca actagaggag aacgtgcagg cgctgaataa tctgacattt
    12241 agcaccgagg agctggcgca gattgatcag catatcgccg atggcgagct gaatctgtgg
    12301 caggcgtcgt ccgataaatg acctgttaat aacgggcgcg cgaaccgcgc ccgaatcaga
    12361 tcagtgatta tgacgagtca gtcggtcaag atagcccatc acaaatgcag aaagcacaaa
    12421 cgtcagatgg ataatgacgt accacatcag tttgttatcc gggacatttt tcgcatccat
    12481 aaagacgcgc agtaagtgaa tggaagaaat tgccacaatc gacgctgcta ctttgttttt
    12541 cagcgacgtt gcgtccattt tccccagcca gttcagcttc tctttgttct cggagatatc
    12601 cagctgtgag acgaaattct cataaccgga aaacatcacc atcaccagca aaccgccaac
    12661 cagcgtcata tccaccagcg acagcaacac gaggatcaaa tctgattccg ccatcgagaa
    12721 gatattcggc agtacgtgaa taatctcctg gaagaacttc agcgccaggg caactaacgc
    12781 cagcgaaagg ccaaagtaca cgggggcaag cagccagcga gaagcataca ttgcattttc
    12841 aagaaaacgt tccatacatt cctgtcgtgt agttaaaacg ggcaacagta tatcgcaatt
    12901 gagtaaacag cacgcaacag caaatcaaca tatacgttaa acctttgtcg cataattctg
    12961 aggtttaggg atcttcctgg ttcccttatc aacgcatcga gagcgtctac ccttaaagga
    13021 cgttgaacct gaagggagaa aaacgatgtc tcatttaaaa gacccgacca cgcagtatta
    13081 cactggtgaa tatcccaaac agaaacaacc gacgccaggc atccaggcga agatgacacc
    13141 ggtaccggat tgcggcgaga aaacctatgt tggtagcggt cgcctgaaag atcgtaaagc
    13201 actggtgaca gggggcgatt ccggaatagg tcgcgctgcc gccatcgctt acgcgcgtga
    13261 aggggctgac gtggcgatca gttatcttcc cgtggaagaa gaagacgctc aggatgtgaa
    13321 aaagatcatt gaagaatgcg gacgcaaagc cgttctgctg ccaggcgatt taagcgatga
    13381 gaaattcgcc cgttcgctgg ttcacgaagc gcacaaggcg ttaggcgggc tggatattat
    13441 ggcgctggtc gccgggaaac aggttgccat tccggatatt gcagacctca ccagcgaaca
    13501 gtttcaaaag acctttgcca ttaacgtttt cgcgctgttc tggctaaccc aggaagcgat
    13561 ccccctgcta ccgaaaggtg caagtatcat caccacttcg tcaatccagg cataccagcc
    13621 aagtccgcat ttactggact atgcggctac gaaggcggcg attctgaact acagccgtgg
    13681 cctggcaaaa caggtcgcgg agaaaggtat tcgggtgaat attgtcgcgc caggccctat
    13741 ctggacggca ctgcaaattt ccggcggaca aacgcaggat aagatcccgc agtttggtca
    13801 gaaaacgccg atgaagcgtg cggggcaacc ggcggaactg gcccctgtat atgtttatct
    13861 ggcaagtcag gagtcgagct acgtcaccgc agaagtgcac ggcgtgtgcg gcggcgagca
    13921 tttaggttaa aaaaatgccc ggttgtgaaa agcaaccggg catcattgtg aattcactct
    13981 tacctgatgt ggcgaaaacg ccttatccgg cctacaaacg cacgaagatt caacatgttg
    14041 caatagattt tgtaggcctg atgagcgtag cgcatcaggc aattctactt tacttcgctt
    14101 tggcggtttc ttcgccgacc agacctatct tcaggtaacc cgcctgatgc agcgtatcca
    14161 ttaccttcat caacgtctcg taatcgacgg ttttgtccgc tcggaagaag atggtggtgt
    14221 ctttcttgcc ttcggttaac gcattcaacg ccgtaatcat tgtttcatcg gtgaccggat
    14281 cgttaccgat aaacatcgag ttgtctgcct tcaccgacag ataaaccggt ttttccgggc
    14341 gcggctgcgg cgtgctggta gaagcaggca agttcacctt cacatctacc gtcgctaacg
    14401 gtgccgccac cataaagata atcagcagaa ccaacatcac gtcgataaac ggcgtcacgt
    14461 tgatatcatg catttcgccg ttatcgtcga ggttttcgtt aagatgcatt gccatcggac
    14521 attatcctgc gcgtaatttt tgtgcgacac gaaccggatg cgcagcggcg ctggcttcca
    14581 gatccaggtc acggctttgc agcaacaata cctgcgctgc aacatcaccc agcatcgctt
    14641 taaagccgcc aatctggcgt gcaaatacgt tatagataac gaccgcagga atcgctgcca
    14701 cgaggccgat tgccgttgct aacagagctt ctgcgatacc cggcgcaacg actgccaggt
    14761 tagtggtctg cgtttgcgcg ataccaataa agctgttcat gatgccccag accgtaccaa
    14821 acagaccaac gaacggagaa atcgcgccga tggttgccag atagccgtta ccgcggccca
    14881 tttgacgacc cactgcggcg acccgacgct ccagacggaa gctggtacgt tctttaatac
    14941 cttcgttatc gtcgctgcct tctgacagtt ccagctcatt ctgcgcttca ttgagcaaat
    15001 gcaggcttaa gcttttgcta ccaaaatcag cggcgatatc gttggcctgg tttaaggaac
    15061 gcgcttcagc cagcagttgc tgctcgcgct taaggcgacg cttctgattg aagaactcta
    15121 cgctcttact gaagaagatt gcccaagtga ctacggaggc caaaataagc ccaatcatca
    15181 cgcacttaac gacaatatcg gcgtgctgat acatacccca gacggaaagg tccgtctgca
    15241 tcaaattatt acccactgtg tatctccagg acgcaagtca caaaatctgc gcataataat
    15301 atcaaaatga cgtcgaattg atagtcgttc tcattactat ttgcatactg ccgtaccttt
    15361 gctttctttt ccttgcgttt acgcagtaaa aaagtcacca gcacgccatt tgcgaaaatt
    15421 ttctgcttta tgccaattct tcaggatgcg cccgcgaata ttcatgctag tttagacatc
    15481 cagacgtata aaaacaggaa tcccgagatg gcggacaaaa agcttgatac tcaactggtg
    15541 aatgcaggac gcagcaaaaa atacactctc ggcgcggtaa atagcgtgat tcagcgcgct
    15601 tcttcgctgg tctttgacag tgtggaagcc aaaaagcacg cgacgcgcaa tcgcgccaat
    15661 ggcgagttgt tctatggacg gcgcggaacg ttaacccatt tctccttaca acaagcgatg
    15721 tgtgaactgg aaggtggcgc aggctgcgtg ctatttccct gcggggcggc agcggttgct
    15781 aattccattc ttgcttttgt cgaacagggc gatcatgtgt tgatgaccaa caccgcctat
    15841 gaaccgagtc aggatttctg tagcaaaatc ctcagcaaac tgggcgtaac gacatcgtgg
    15901 tttgatccgc tgattggtgc cgatatcgtt aagcatctgc agccaaacac taaaatcgtg
    15961 tttctggaat cgccaggctc catcaccatg gaagtccacg acgttccggc gattgttgcc
    16021 gccgtacgca gtgtggtgcc ggatgccatc attatgatcg acaacacctg ggcagccggt
    16081 gtgctgttta aggcgctgga ttttggcatc gatgtttcta ttcaagccgc caccaaatat
    16141 ctggttgggc attcagatgc gatgattggc actgccgtgt gcaatgcccg ttgctgggag
    16201 cagctacggg aaaacgccta tctgatgggc cagatggtcg atgccgatac cgcctatata
    16261 accagccgtg gcctgcgcac attaggtgtg cgtttgcgtc aacatcatga aagcagtctg
    16321 aaagtggctg aatggctggc agaacatccg caagtagcgc gagttaacca ccctgctctg
    16381 cctggcagta aaggccacga attctggaaa cgagacttta caggcagcag cgggctattt
    16441 tcctttgtgc ttaagaaaaa actcagtaat gaagagctgg cgaactatct ggataacttc
    16501 agtttattca gcatggccta ctcgtggggc gggtatgaat cgttgatcct ggcgaatcaa
    16561 ccagaacata tcgccgccat tcgcccacaa ggcgagatcg attttagcgg gaccttgatt
    16621 cgcctgcata ttggtctgga agatgtcgac gatctgattg ccgatctgga cgccggtttt
    16681 gcgcgaattg tataacattg ccacttttgg acaattttgc agacatttaa ttgtgaaaag
    16741 tcttaaattg ttgcgtccgg gatcaaggcg tcccggacga atcaggagta caataggcag
    16801 ataaaggctt aaacgctgtt ccacaggaaa gtccatggct gttattcaag atatcatcgc
    16861 tgcgctctgg caacacgact ttgccgcgct ggcggatcct catattgtta gcgttgttta
    16921 ctttgtcatg tttgccacgc tgtttttaga aaacggcctg ctgcccgcct catttttgcc
    16981 aggcgacagc ttgttgatac tggcaggcgc attgattgcc caaggggtta tggattttct
    17041 gcctacgatt gcgattctga ccgccgcagc aagtctgggc tgctggctaa gttatattca
    17101 ggggcgctgg ttagggaata ccaaaacggt gaaaggctgg ctggcacagc ttcctgctaa
    17161 atatcaccag cgcgccacct gcatgtttga ccgccacggt ctgctggcgc tgctggctgg
    17221 acgttttctt gcatttgtcc gtacgctgct gccaaccatg gcgggaattt ccggtctgcc
    17281 aaaccgccgc ttccagtttt tcaactggtt aagtggattg ctgtgggtca gcgtggtaac
    17341 cagttttggc tatgccttaa gtatgattcc gttcgttaaa cgccatgaag atcaggtaat
    17401 gacgttcctg atgatcctgc caattgcctt gttaaccgct ggcttgttag gcacgctgtt
    17461 tgtagtgatt aaaaaaaaat actgtaacgc ctgacgattt tccccgttcc cggctgctgt
    17521 accgggaacg tatttaattc ccctgcatcg cccgcattct tgccgcatct tcccccggcg
    17581 tcacaccgaa gtaacgttta aactcacggc taaattgcaa tgcgctttca tagtcgacgc
    17641 gcatcgctgc tgcgctggcc ttcatgccgt catggatgat catcatccgc gccttatgca
    17701 gacggtaatt cttcaaatac tgcaacggcg aggtgctggt gacagactta aaattatgat
    17761 ggaacgccga tacgctcatg ttggcttctg ccgccagttg ctcgacgctc aggttttcgg
    17821 tgtatttatt ctcaatccgt ttcagcacgc ggctaatcag actgaagtga gtctggcgac
    17881 tgaccagcgc cagtaacgcg ccgccgcaag gtccggtcag cacgtagtac agaatttcgc
    17941 ggatgatctg tttgccgaga atacgcgcat ccagtggtcg ctccatcacg tcgagtaacc
    18001 gctccgccgc gcataaaatc tcttctgata acgtggcgga gttaatcccg ctggctgcca
    18061 tcgacggctg gaaatgctca tcttcgccaa tgtccatcaa cagttcctgt aactgcaaaa
    18121 tatcgacatt gagacgcaat cctgccagcg gcacctctga cgtggcatag gtttcgcact
    18181 caaacggcaa cggcaccgtc agcagcaggt attcattggc atcataacga aacacgcgtt
    18241 cattgatata accgatttta tgcccggaaa agagaattat gatgccaggc tcgtacatca
    18301 ccggtgtacg tgcgaaaggc gtctcgccat acaacaaacg cacatcgggc aacagttctg
    18361 acaaactatt ttctttattt ttcagtttat taactttatc cgccagcaag cggcaaatct
    18421 cttcacgttt catatcgcgt aatttcttag gaataatgcg gcaatttgat tgtgcgcaat
    18481 tttgtagcat ttctccagca ctctggagaa ataggcaaga cattggcaga aatgagcatt
    18541 gagagccagg gcgctggcga tcacaatgaa aaacatcagg cagatcgttc tctgccctca
    18601 tattggccca gcaaagggag caagtaatga acaactttaa tctgcacacc ccaacccgca
    18661 ttctgtttgg taaaggcgca atcgctggtt tacgcgaaca aattcctcac gatgctcgcg
    18721 tattgattac ctacggcggc ggcagcgtga aaaaaatcgg cgttctcgat caagttctgg
    18781 atgccctgaa aggcatggac gtgctggaat ttggcggtat tgagccaaac ccggcttatg
    18841 aaacgctgat gaacgccgtg aaactggttc gcgaacagaa agtgactttc ctgctggcgg
    18901 ttggcggcgg ttctgtactg gacggcacca aatttatcgc cgcagcggct aactatccgg
    18961 aaaatatcga tccgtggcac attctgcaaa cgggcggtaa agagattaaa agcgccatcc
    19021 cgatgggctg tgtgctgacg ctgccagcaa ccggttcaga atccaacgca ggcgcggtga
    19081 tctcccgtaa aaccacaggc gacaagcagg cgttccattc tgcccatgtt cagccggtat
    19141 ttgccgtgct cgatccggtt tatacctaca ccctgccgcc gcgtcaggtg gctaacggcg
    19201 tagtggacgc ctttgtacac accgtggaac agtatgttac caaaccggtt gatgccaaaa
    19261 ttcaggaccg tttcgcagaa ggcattttgc tgacgctaat cgaagatggt ccgaaagccc
    19321 tgaaagagcc agaaaactac gatgtgcgcg ccaacgtcat gtgggcggcg actcaggcgc
    19381 tgaacggttt gattggcgct ggcgtaccgc aggactgggc aacgcatatg ctgggccacg
    19441 aactgactgc gatgcacggt ctggatcacg cgcaaacact ggctatcgtc ctgcctgcac
    19501 tgtggaatga aaaacgcgag accaagcgcg ctaagctgct gcaatatgct gaacgcgtct
    19561 ggaacatcac tgaaggttcc gacgatgagc gtattgacgc cgcgattgcc gcaacccgca
    19621 atttctttga gcaattaggc gtgccgaccc acctctccga ctacggtctg gacggcagct
    19681 ccatcccggc tttgctgaaa aaactggaag agcacggcat gacccaactg ggcgaaaatc
    19741 atgacattac gttggatgtc agccgccgta tatacgaagc cgcccgctaa gctttttacg
    19801 cctcaaactt tcgttttcgg gcatttcgtc cagacttaag ttcacaacac ctcaccggag
    19861 cctgctccgg tgagttcata taaaggagga acgtatggct aatccaaccg ttattaagct
    19921 acaggatggc aatgtcatgc cccagctggg actgggcgtc tggcaagcaa gtaatgagga
    19981 agtaatcacc gccattcaaa aagcgttaga agtgggttat cgctcgattg ataccgccgc
    20041 ggcctacaag aacgaagaag gtgtcggcaa agccctgaaa aatgcctcag tcaacagaga
    20101 agaactgttc atcaccacta agctgtggaa cgacgaccac aagcgccccc gcgaagccct
    20161 gctcgacagc ctgaaaaaac tccagcttga ttatatcgac ctctacttaa tgcactggcc
    20221 cgttcccgct atcgaccatt atgtcgaagc atggaaaggc atgatcgaat tgcaaaaaga
    20281 gggattaatc aaaagcatcg gcgtgtgcaa cttccagatc catcacctgc aacgcctgat
    20341 tgatgaaact ggcgtgacgc ctgtgataaa ccagatcgaa cttcatccgc tgatgcaaca
    20401 acgccagcta cacgcctgga acgcgacaca caaaatccag accgaatcct ggagcccatt
    20461 agcgcaagga gggaaaggcg ttttcgatca gaaagtcatt cgcgatctgg cagataaata
    20521 cggcaaagcc ccggcgcaga ttgttatccg ctggcatctg gatagcggcc tggtggtgat
    20581 cccgaaatcg gtcacacctt cacgtattgc cgaaaacttt gatgtctggg atttccgtct
    20641 cgacaaagac gaactcggcg aaattgcaaa actcgatcag ggcaagcgtc tcggtcccga
    20701 tcctgaccag ttcggcggct aacatgcaaa ttctcccggt gacggtaatg ttccgctacc
    20761 ggacttttca gaaatcattt attcccctcg cgtcccgccc gttgttactc ttccttgttc
    20821 aggaatgcca aatataagga catcatcatg cagagccgga agctcttaaa agaacaactc
    20881 atctatatcc gggataaacg caacggagag gtgaaaaaca gatgaaaata atacttctgt
    20941 ttttagcagc cctggcaagt tttaccgtac acgcgcagcc tccttcacag accgtagaac
    21001 aaacagtccg gcagatttat cagaactata aatcagatgc cagtacacct tatttcggtg
    21061 aaaccggaga gcgggcgata acttcagcgc gtattcaaca ggcgcttacc ctgaacgaca
    21121 atcttacgct gccgggcaat attggctggc tggattatga tccggtttgt gattgtcagg
    21181 attttggcga tctggtgcta gaaagcgttg cgataaccca aactgacgcc gatcatgccg
    21241 atgccgttgt gcgctttcgt atctttaaag atgataaaga aaagaccacg cagacactga
    21301 aaatggtggc ggaaaatggt cgttgggtca ttgacgatat tgtcagcaat catggcagcg
    21361 tcttacaagc agttaatagc gagaatgaaa aaacgctggc cgctttagct tcgttgcaaa
    21421 aagaacagcc ggaagccttt gttgccgaac tctttgaaca tattgctgat tatagctggc
    21481 cgtggacgtg ggtggtttcc gactcttacc gccaggcggt taatgccttc tataaaacca
    21541 ccttcaagac ggccaataat cccgatgaag atatgcaaat agaacggcaa tttatttacg
    21601 acaatccgat ctgttttggc gaagagtcgc tattttcacg cgttgatgaa attcgagtcc
    21661 tggagaaaac cgccgattcc gcccgcattc atgttcgttt tacgctgacc aatggcaaca
    21721 acgaagagca agaactggtt ttacagcggc gcgaaggcaa gtgggaaatc gctgatttta
    21781 tccgcccgaa cagcggcagc ctacttaagc agattgaggc aaaaaccgcc gccagattaa
    21841 agcaatgagc tgaattaaat aacaattagc cggaacaata aataaaaggg aacactatat
    21901 gaaaacgatt ttcaccgtgg gagctgttgt tctggcaacc tgcttgctca gtggctgcgt
    21961 caatgagcaa aaggtcaatc aactggcgag caatgtacaa acattaaatg ccaaaatcgc
    22021 ccggcttgag caggatataa aagcactacg cccacaaatc tatgctgcca aatccgaagc
    22081 taacagagcc aatacgcgtc ttgatgctca ggactatttt gattgcctgc gctgcttgcg
    22141 tatgtacgca gaatgataaa aaaatccccg gcagcatgtc agttgccggg gatttttttt
    22201 aacgtccaac cgccgcttta gggcgtttct tcgcaccagc attcaccgga cgagattgcg
    22261 tagacgacgc tttttttgcc gtagcaggcg tctgacgctg ggtcgccatc ggcgtatgtt
    22321 tagtcaacgc cggacgggta ttgcggttct ggcgacgagc ttcacgcatc tcttcaatgg
    22381 ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag atgctttttg cccatcgctt
    22441 ccagcgccgg gcggattaac ggccagtttg ccggatcgtg gtaacgcaac aacgctttat
    22501 gcaaacgacg ctgtttgtcg cccttcggta cgaagacgtc ttcgctctta taaccaatct
    22561 tcgccagcgg ttttttcccg gtgtaataca tggtggttga gttcgccagc ggcgatgggt
    22621 agaagttctg tacctggtcg agacggaaac gatgcttttt cagccacagc gccaaattca
    22681 ccatatcttc atcacgcgta ccggggtgcg cagagatgaa atacgggatc agatactgct
    22741 ctttacctgc ctgtttcgag taggtatcga acagctcttt aaagcggtca tagctgccca
    22801 tgcccggctt catcatcttc gacaacggcc cttcttcggt atgttccgga gcaatcttca
    22861 gataaccgcc gacgtgatgg gtcgccaact ctttgatata gcgcggatct tccacggcga
    22921 tgtcataacg tacgcctgag gcgatgagga tttttttaat gcctttcaga tcacgcgcac
    22981 ggcgatagag gttgatcgtc ggttcgtggt tagtgtccat gtgcggacaa atatccggat
    23041 aaacgcacga caaacggcga caagtttgtt cagcgcgtgg cgatttgcag cgcaacatat
    23101 acatgttggc agttggccca ccgagatcgg aaatcacgcc cgtaaaacct ggaacggtgt
    23161 cgcggatcgc ttcgatctca ttaatgatcg aatcttccga acggctctga ataatgcgcc
    23221 cttcgtgctc ggtaatagaa cagaaagaac agccgccaaa gcagccgcgc ataatgttga
    23281 ccgaaaaacg gatcatttcg taagccggaa tacgggcatt gccataggcc ggatgtggca
    23341 cgcgcttgta cggcagcgca aaaacgctgt ccatctcttc ggtagaaagc gggatagcag
    23401 gcgggttgat ccacacatag cggtcaccgt gtttttgcat caatgcgcgg acgcagcctg
    23461 ggttggtttc gtggtgcaga atacgcgaag catgggcgta cagcacttta tcgcccttca
    23521 ctttctcgaa agaaggcagc aacacgtagg ttttttccca cggtttcggg cgcggtggct
    23581 gcacggttac ggctttggct tcctgctttt tcggtgccac cggtttgtta tccgcgcacg
    23641 gcaaatcttc accatacgga tgcgggattg ggtcgatttt tccaggggta tcaagacggg
    23701 tggaatccac gccgctccag ccaggcagcg cctctttcac gataatcgcg gtattacgca
    23761 catcgcggat ttcactaatc ggctcgccca tcgccagacg gtgcgccacc tccaccagcg
    23821 gacgctcacc gttaccaaac atcagcatgt cggctttcga atccaccagc acggaacggc
    23881 gcacggtatc ggaccagtaa tcataatgtg cggtacggcg cagactagcc tcaataccgc
    23941 cgaggatcac cggtacatct ttccacgcct ctttacaacg ctgggtataa accagtgtgg
    24001 cgcgatccgg gcgcttaccc gcgacgttat ccggcgtgta ggcatcgtca tgacgtaaac
    24061 ggcgatcggc ggtataacgg ttgatcatcg aatccatgtt gccagcagta acaccgaaaa
    24121 acagattcgg tttacccaga cgcataaagt cgtctttgct gctccagtcc ggctgggcga
    24181 tgatcccgac gcgaaagccc tgtgcttcca gcatacgacc gcaaatcgcc atcccgaagc
    24241 ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta tcccagccaa
    24301 gttgatccat ctcttcacga gacatcggca aaaacggtgc cggtccaaaa caggcggccc
    24361 agtactgcgg ccaggagaac aggtcgcgat ccggttggat cagggagata gagctcattt
    24421 tgcttccaga aatgataaaa aaataatcaa aggccgggga ttataagccg gaacgaaaga
    24481 gaaatcgaaa agtattccat actcgccctc ctcgggcgag tatgaagatt acggtaccgg
    24541 attgactaac agttgcccaa tcgagccacg gtccgccatt tccagcgtct gactgttgaa
    24601 gtagaacggg aagtgcgccc aggaaggctg accgaaataa acaagcagct ccacttgtcc
    24661 gtcaacccaa acggtatctt tccagcctct gtcttccggg aacggcatcg caccattcac
    24721 gttacggatc tggaacatca cgccttcaat atggaacgct tgcggctcgt ccgcgcgtac
    24781 cgtccagcgt tcccacgttc cttgctgcgc ggtgacatca atacggttga cgtcccacag
    24841 ctgtccatta atacccgggt catcacccag actgatatcg cgactgcgaa tcggcgaacc
    24901 ggccatgatt tcagttggca gcaagcgcat cggaagactg tctgtgacca gcggcagaag
    24961 gccggttggg cgcagcgtta gcaccagggt agaaaccaga atactggatg gctcaaagaa
    25021 gccgcgaata cgatcaacaa tgctcgccgc ttcgccacag gtgatcgaca cttcatcgcc
    25081 gttgctcata tccaccagaa tctcgcggcg ctcgcccggt gccagcgaaa gttgcttcac
    25141 cgatacagga gcaggcagga atccctgatc gccagaaatc acatgtaacg ggcgaccatc
    25201 gctcatctgt agttgatagc gacgagagtt cgacgcgttc agcagtcgca agcgcaccca
    25261 gccacgcgag acttcaacgt acgggctttg tacaccgtta accagcagcg tatcaccaac
    25321 aaagccgccg cttcccggtt cgttgtattc tggcgtacca aagttatcca gccgtttatc
    25381 ctggataatg accggaaaat catccacatc ataatggttg gggataggca gcgacttgct
    25441 gacttcatct tccaccagcc acattccggc aaggccgtta tagacctgct gagccgtgcg
    25501 gttgggagta ttggcgtgat accacagagt agctgcgttc tggcgaatag gcagtacggg
    25561 tgcccagtca gcgtttggcg acatcatccg tgccggaccg cccatcagcg ggccaggtac
    25621 ctgtagcccg gccaccgtca ttgagacatt ttctgtcagg cggttgctgt aaataagctt
    25681 aacatcgtcg cccttccaga cgcggatagt cggccccagg taacgaccat tgattcccca
    25741 gaccgacgcg cgtgtccctg gcgtaaatga ccagtgcgca cgttgtacag tcataaacag
    25801 cggttgccca cggcgagatt caagtagcgg cggaacgggt agcggttgct gttgcccggc
    25861 tgcgctggcc ttcaggggaa cagcgcctgc acaaagtgca atccccgatg cctgaatgaa
    25921 ctgacgccga ctgagtgaca tatttgctcc atgtaaaact ggctaaagta ataccgtgca
    25981 ggaaagtgtt ccccgcaaaa atacagattg ccgacttaaa cttttccggc ggcttcgcgt
    26041 tctgcgactt ctttatcgag ctcggcgatt ttttgttcca ttatcgaacg acaatgggca
    26101 gccagctcac gaacctgatc tttgccatac tgactgacgt caattggcgg cagcatttcg
    26161 acaatcacca gaccgttgtg cagtcgatta agattaatct tattcgaagt tgtagagacg
    26221 cacacgggaa taatcgggac gcccgccgca attgccgcgt gaaatgctcc agtcttgaac
    26281 ggtagcaggc cgcgaccacg gctgcgggtt ccttccggga acatccagat ggaaatacgg
    26341 cgttttttga aatgattcac gacttccgca atggtgccgt gagctttagt gcgattgttt
    26401 ctgtcgatca ataagttgcc ggttaaccag tacaactgcc cgaagaaggg gatccacagc
    26461 aagctctttt tacctaccgt caccgtcggc ggttgcacaa tgttcgatgc tgtcaccatg
    26521 tcatagttgt tctggtggtt agcgatatag atagcattgc cgtagctttc agcatctgca
    26581 ggtttgcggc actcaacttt caggccgaac agcggcgcaa gacggccaaa catatgccca
    26641 aaggtggcca catgtttcgg gttacgcggg ctgaaaaggc agtaaatgga gccgaataca
    26701 cagactaaga tgctgtaaat cacggtaata ataagacgaa agatatatag cataacaccc
    26761 tctgaaggtc tgagagactg gcattgtacg tcacctgaaa tcaggttagg gcattgttaa
    26821 aagccgctct ctggaaaagc gtattgttaa tcgcaacgca tgaataaaca acggtttcaa
    26881 gggaattttt atcgtttccg ggggttgaat acgctgccgg atgacgactt aacgtttcat
    26941 ccggcgttcc ttgcaagcgg gaggaaacag gccctccccg gcatattact cttcgctatc
    27001 accgttgctg gcacggcgag gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg
    27061 catcaacgta ccgcggcgtc cacgttcacc agtgactttc tgtaactctt ccgggcgcag
    27121 tttaattttg cgtttcccaa catgaatggt cagcgtgctt tgcggcggca gaacgtacaa
    27181 ttgcgccaga ccatcttctc cacgcgcggc ttctgccgat ggaatgttga taatcttgtt
    27241 gcctttgccc ttcgacagct gcggcagatc gcttaccggg aacatcaaca tacggcctgc
    27301 ctgagtgatt gccagcagca tatcggaagc atcttcaatc accaccggcg gcataacatg
    27361 ggcattttcc ggtaaggtga tcaaagcctt acctgcacgg ttacgcgcca ccagatcgtt
    27421 aaaggtgcag acgaaaccgt aacccgcatc ggaagccatc agcagtttct gatcgtcgct
    27481 ttccatcagc atatggtcaa cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg
    27541 ttcgccctga ccacgcgccg acggcagcgt aatcgggtcg atggcatagc tacgaccggt
    27601 ggaatcaaca aacactaccg attggttgct cttacctttc accgccgctt tgaagctatc
    27661 acccgcttta taattcaggc ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca
    27721 gcccatctgc gacagcacaa tggtgacagg ttcagacggc agcatgtcgt gctcgctcat
    27781 cgctttcgct tcttcgcgtt cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc
    27841 gtctgcctgc agttctttct tcagcaggtt attcatttta cgctcggaag ccaaaatgcc
    27901 ctgcaactgg tcgcgctctt tttccagctc actctgctca ccgcgaatct tcatctcttc
    27961 cagtttggca agatgacgca gtttcagttc gaggatcgct tccgcctggg tttccgtaag
    28021 gccaaaccgc gacatcagcg ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac
    28081 ttcgtcgatg ttgagaaacg ccaccagcaa accttccagg atatgcaggc gcttgaggac
    28141 tttctccaga cgatagttca gtcggcggcg cacggtatcg cgacggaaca ccagccattc
    28201 ggagaggatt tccagcaggt ttttcaccgc cggacgacca tccagaccga tcatattgag
    28261 gttaatgcga tagctctttt ccagatcggt ggtagcgaag aggtggttca tcacctgatc
    28321 catatccacg cggttggaac gcggcacaat caccagacgg gtcgggttct cgtggtcaga
    28381 ttcatcgcgc agatcgtcaa ccatcggcag ctttttgttg cgcatttgcg cagcaatttg
    28441 ctccagtaca cgcgcacctg aaacctgatg cggtaatgcg ctgataacca ccgcgccatc
    28501 ttctttcttc cacaccgcgc gcatacgcac tgaaccacgt ccgttctcgt agattttacg
    28561 gatctcggcg cgcgaagtga taatttccgc ttcagtcgga taatccggcc cctgcacgat
    28621 atccagcagc tgatcgagcg tggttttcgg ctggtcgatt aatgcgattg ccgcctgagc
    28681 tacttcacgc aggttatgcg gtggaatatc ggtcgccatg ccgacggcaa taccggtggt
    28741 gccgttaagc aaaatgtttg gcagacgagc aggcagcatt ttcggctcct gcaaagtgcc
    28801 gtcgaagttt ggcacccagt cagccgtccc ctgccccagc tcgctcaata gcagctcgga
    28861 atatttcgac aaccgggatt cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg
    28921 cgcgccccag ttcccctgac catcaaccag cggataacgg taagagaacg gctgcgccat
    28981 caggaccatc gcttcataac aggcgctatc gccgtgcgga tggtatttac ccagtacgtc
    29041 accgacggta cgggccgatt ttttaaattt ggcgctggca ttcaggccca gttcagacat
    29101 cgcatacaca atgcggcgct gaacaggttt cagaccatca ccaataaacg gcaacgcacg
    29161 gtccatgatc acgtacatgg agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc
    29221 aaggcgctct gccatatcgc tcattaattc tgattcctca actgattcgc cagccttcaa
    29281 aggggcaata ttgccggaga tactacatta tctgccgcgt cgagtcacag gagatcagcg
    29341 tgaatagcgc cgccggacgt gctccgacgg cgataaaggc atcaatgtgc cttgatatac
    29401 aactctttgc tgtagacttg atcggcatag agggcagcac aaggttgcgc ccatgccaca
    29461 ccactcggca tgggggtccg ctccgggcga ttcgagaagt tgaggttatg cgagtccagg
    29521 gaagcctaaa gcatcaaagt agtcgactgt aagtatatgg ttcagcccag tttagctgtg
    29581 tctccaccag tgccccgcgc ctcctgccat aagtgcggct tgctcatggg tacccccgag
    29641 acggcttacg gcgcaccgga taataaattt cgcgtataca tggtattcct taccggagaa
    29701 attataagcc caacaacaat acgcctgtgg cattgttcgc tcaaacttat cgcgagtgat
    29761 ttggctcaca ctccggtaaa gattccccat cacgctaacg gcacataaac atccgtttcc
    29821 agtcgctgct ccgtcacccc ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta
    29881 cgcattttct cgccgcttgc aggcagccag tggcgaataa tgccccatat cgtgtgtgaa
    29941 atatcgtcca gctcgccaac gtggcgggcc acggcataac gtccaccggt aagttcacca
    30001 ttgctcacac cataacgatt atcgggaatc ggttcgctaa cgctgccgca gatatcaaag
    30061 cgaaacgctt ccggtgcggt ggtggcaggg tcgtcccagg cgacgccaaa agtctggctt
    30121 tggttaacag gagaaagccc cgtctcttta cgccacatga taaatttcgc cacactgtag
    30181 ttgagcaact cagggctaca gcggtgcggc aacatcgcca cagggattga tgggaaatcg
    30241 ataatgttta catccagtgt caggtttgtc attttgcctc cctgcgaaat tattcccggg
    30301 aacgcgtccc gggagcggta acaattacgg atttactttg cggatctgtt tgacgtcaat
    30361 ttcaacagaa ttccagtctt tatcgacttc accctgaatc tcaaccgtat ctttcggcgt
    30421 caccgtcaca ccgttccagc gtttgtggtc gatatcaaca ttgatagtac cgctggcatc
    30481 tttaaacacg tagagatcgt cagagatgcg ttcaacgata ttgccgcgca gggttaccca
    30541 ggtgtcgtca cgcagggatt ttgcgctttc tacagtcgtt acgctgccgt tcggcccctg
    30601 gaatcctccg gcctgacttt gcgttgccga tgggccagaa aaaccgccct gctctgctgc
    30661 catcaccggt gcactgcaca gggccattac tgcgattact gctgcgaatt ttttcatgtt
    30721 tattactccc tttaatgtct gtttccgagc atttaacaag atagtcctta acaacttctt
    30781 aagggaaaaa aataaaattt aatgctgtac agagcgcgtt acaacacggt ttactggcag
    30841 caaatacggt tatcgcaggg atgaaaaaat gcgaatttta ctgatagaag atgacatgct
    30901 gattggcgac ggcatcaaaa ctggccttag taaaatgggt tttagtgtcg actggtttac
    30961 acaaggtcgt cagggaaaag aggcgcttta tagcgcacct tatgatgcgg tgatcctgga
    31021 tttaacctta ccaggcatgg atggtcgcga tattttgcgc gaatggcgag agaaaggtca
    31081 gcgtgagccg gtactgatcc tgaccgcgcg tgatgcgtta gcggaacgtg tagaagggct
    31141 gcgtctggga gctgacgatt atctgtgtaa accttttgcg ttgatagaag tcgccgccag
    31201 gctggaggct ctgatgcgcc gaaccaacgg ccaggccagc aacgagctgc gccacggtaa
    31261 cgtcatgctc gaccccggca aacgtatcgc cacgctggct ggcgaaccct taacactgaa
    31321 accaaaagaa tttgccctgc tggaattact gatgcgtaac gctggtcggg tactgccgcg
    31381 caaactgatt gaagagaaac tgtatacctg ggacgaagag gtcaccagta atgccgttga
    31441 agtgcatgtg catcatctgc gacgcaaact cggtagtgat tttattcgta ccgtgcatgg
    31501 tattggctac acattaggtg agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg
    31561 ctgacgctaa tctttttaat tctggcctcg gtgacctggc tgctttccag ctttgtcgcc
    31621 tggaaacaaa caacggataa cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc
    31681 aagcggttaa gtacgctcga tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg
    31741 ccaaataaat taaaacacgg tcatgttgat gacgatgcgc tgacctttgc catctttacc
    31801 cacgacggca gaatggtcct taatgatggc gataacggag aagatattcc ctatagctat
    31861 caacgggaag gttttgctga cgggcaactg gtcggtgaag acgatccttg gcgttttgtc
    31921 tggatgacct cacctgatgg caaatatcgc atcgttgttg gccaggaatg ggaataccgt
    31981 gaagacatgg cgctggcgat tgttgccggg caattgatcc cgtggctggt cgcactgccg
    32041 attatgttaa tcatcatgat ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg
    32101 gcgctggcac tacgtatgcg tgaccctgac tcggaaaaac cactaaacgc gactggcgta
    32161 cccagcgaag tgcgaccact ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg
    32221 atgatggttc gagaacgacg ctttacctcc gatgcagctc acgaactacg tagcccgtta
    32281 acagcgctga aagtgcaaac cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg
    32341 aaaaaagcac tgctccaatt acattccggg atcgatcgcg ctactcgtct ggttgatcaa
    32401 ctgctcacgc tatcgcggct ggactcactg gataaccttc aggacgtcgc ggagatcccg
    32461 cttgaagatc tcctgcaatc gtcggtgatg gatatttacc acacggcgca gcaggcgaaa
    32521 attgacgtgc gactgacact caatgcccac agcatcaaac gcactggaca accgctattg
    32581 ctaagtttgt tggtgcgaaa ccttctggat aacgccgtgc gctacagtcc acagggcagc
    32641 gtggtggacg tcacgctgaa tgccgataat ttcatcgtga gggataacgg ccccggtgtg
    32701 acaccagagg cactggcgcg aattggcgaa cgcttctatc gcccccccgg acaaaccgct
    32761 accggcagtg gacttgggct atcgattgtc cagcgaatcg ccaaattgca tggcatgaat
    32821 gttgaatttg ggaatgcgga acaaggtgga tttgaggcga aggtgagctg gtaagggtaa
    32881 gacttttgct aaattcgcaa aagactttgc acattttgct aattttaccg taccgctctg
    32941 tgacgtacta tagtcgacaa acgtctcacc ttgaggttaa aaaatgagca acaccctgat
    33001 tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca ccctgaccga
    33061 agtcgcggat ggcacactgc gcgacctcgg gcatgatgtt cgcatcgttc gcgccgacag
    33121 cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg tgatctggca
    33181 gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa aaatacattg atgatgtatt
    33241 caccgaaggt cacgggacgc tgtatgccag cgatggccgt acccgcaaag atccgtcaaa
    33301 aaaatacggt tccggcggcc tggtacaggg caaaaaatat atgctttctc tgacctggaa
    33361 cgcaccaatg gaagccttca ccgaaaaaga tcaattcttc cacggcgttg gcgtcgacgg
    33421 tgtgtatctg ccgttccata aagcaaacca attccttggc atggaaccgc tgccgacatt
    33481 tatcgctaat gacgtgataa aaatgcctga tgttccccgc tatactgaag aatatcgcaa
    33541 gcatcttgtg gaaatttttg gttaactaga gctcaggctt tagaaggagt taaccatgct
    33601 taccgtaatc gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg cggtattgga
    33661 tcagtttgct aaaatcgttc caaccgtact gaaagaagaa ggttgccacg gctatgcgcc
    33721 aatggtggat tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt ctatcgtgat
    33781 gattgagcag tgggaaagca tcgcgcacct tgaagcgcat ctgcaaaccc cgcacatgaa
    33841 ggcgtacagc gaagccgtaa aaggcgacgt gctggagatg aatattcgta ttctgcagcc
    33901 agggatttaa tcctgccttg tttgcccggc catcctgacc gggcaatgtt ctttccttta
    33961 aacctcaatc tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat cttccgagcg
    34021 tttcttcgcc agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt catcatcgat
    34081 agtcaactgc accagacggc gagtgttcgg atcaagcgtg gtttcacgca attgcatcgg
    34141 gttcatttcc cccagacctt taaaacgctg gacgttcggc ttgcctttct tgcgttttaa
    34201 ttgctcaagt acgccctctt tctcttcttc cgtcagcgcg taataaacct ctttcccgag
    34261 atcaatacgg tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca ccaacgcgcg
    34321 gaagtgtttt acgaacaaag cgcagagcag cgtggcaatg tgcagaccat cagagtccgc
    34381 atccgcgagg atacagattt tgccataacg aagctggctc agatcgtcgc tgtcaggatc
    34441 gataccgatc gctaccgaaa tatcatgcac ttcctgcgag gccagcactt cgtcggaaga
    34501 aacttcccag gtgttaagga tcttaccttt cagcggcatg atcgcctgat attcacgatc
    34561 gcgcgcctgc ttggcagatc cgcctgcgga gtcaccttcc acgaggaaca gttcggtacg
    34621 gttaaggtcc tgcgcggtac aatcagccag tttgccaggc agcgccgggc cgctggtcag
    34681 ctttttacgc accacttttt tagccgcacg catacgacgc tgggcgctgg aaatcgccat
    34741 ctctgccagc aactccgccg cctgaacgtt ctggttcagc cacaggataa aggcatcttt
    34801 caccacgcca gaaacgaatg ccgcgcattg acgcgaagag agacgctctt tcgtctgccc
    34861 ggcaaactgc ggatcctgca ttttcaccga cagaacatag gcgcagcgat cccagatatc
    34921 ttccgccgac agcttcacgc cgcgcggcag aatgttgcga tattcacaga actcacgcat
    34981 cgcgtccaac aggccctgac gcaggccatt aacatgggta ccgccctgca tcgttgggat
    35041 caggttgacg tagctttcgg tcagcagttc accgccttcc ggcagccaca gtagcgccca
    35101 gtccacagct tcagtatcgc cagcgaaatt accgataaac ggtttttctg gcagcgtcgg
    35161 taaaccgttt accgcttccg ccaggtaatc attcagaccg tcctgatagc accagcgctg
    35221 ttcggtgttg ttgatctcat ctttaaaggt aatctcaacg ccggggcaca gtaccgcttt
    35281 ggctttcagc acatgcgtca ggcgtgaaac agaaaatcgc gggctgtcaa agaaggtttc
    35341 atccggccag aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc cgacaacctg
    35401 taaatcctgc accttttcgc cattttcaaa ggcgatgtta taaacctgac catcgcggcg
    35461 cacgttaact tcaacgcgct tcgacagggc gttaaccacc gaaatcccca cgccatgcag
    35521 gccgccagag aactggtaat ttttgttaga gaatttaccg cctgcatgca gacggcaaag
    35581 aatcaattca accgccggga caccctcttc cgggtgaata tccaccggca tcccgcgccc
    35641 atcgtcaata acttctaacg actggtcagc atgtaagatt acatccacgc gttttgcgtg
    35701 acccgccagt gcttcatcga cactgttatc aataacttct tgccccaaat ggttagggcg
    35761 agtggtatcg gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg tgagtacctc
    35821 aatggcatca gcgttataag tttgcgtcat ggtttaagtt agcaattcga gttgatcgtc
    35881 agagatggtg cagaccaaga aaatcgacga tcgggttgaa ataatcttcg aagcccgtga
    35941 atgcgtggtt gccgccttct atgacagtct ggcggcagga agcgtagtac gccaccgcct
    36001 ggcggtaatc cagcacttca tctcccgtct gttgcagcag ccagatcaaa tccggcgctt
    36061 ccagcgggtc aatctgcatg actttgagat cgtaaatatg gcgtgactct agcacatatt
    36121 gctgcccggt gtatgggttc tcgttctgac cgagatagtc cgtcagcagt tcaaacgggc
    36181 gcaccgccgg gtttaccacc actgcgggca gcataaaaca ttgtgacaac caggtggcgt
    36241 aatatccccc cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt tccaggacaa
    36301 tggattccag cagttctgcc gcgtcggaag gatacggcgg caactgcgga atgatcatct
    36361 caacgtcagg gtgatgttcc gccagccagt tttttaacaa gctcgctttt gcagagcgcg
    36421 gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt ctgaagcggt
    36481 atcaggttgg aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg tgccatcagc
    36541 atgtaactcg agagtacgcc agccgggcgc gatagtatcc agcgtaaagt tggaacagtg
    36601 cggcttaaac tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt tccagtcgag
    36661 atccagctcc tgatgaatat gaccgcacag caagtatttg acgtgcggaa acttcgccag
    36721 cacggtatcc agttcgcccg cgttacgcag actgtgttga tcgagccaac tacaacccgc
    36781 aggtagcgga tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat cggccagttt
    36841 acgttccagc cactcaagct gaaactcgct cagctcaccg tgcggcacgc caaacacctg
    36901 gctatccagc aacaggattt gccattgctc accaataaac acccgcttcg ccggggagat
    36961 acccgcatcc tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc ccggcagcca
    37021 gacgcagggc gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg ccgcagagga
    37081 ttgatcctgc gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct gatgtggacg
    37141 aatcgcctcc agcaccgcct ggtagctttc ccaggtgttt acccctaaca gagcttcgtg
    37201 cttttgtgca aacaggtgag tgtcggtaat ttgtaaaatc ctgactctgg cctcaccagc
    37261 cagaggaagg gttaacaggc tttccaaatg gtgtccttag gtttcacgat gctagtaaac
    37321 cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac cagtccgcta aaaactgatt
    37381 aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta ggataatcat accgcgcttt
    37441 gaagcgaaaa atctgctggc ttgaacacac ttcagccacc atcgcgtcat gatacagacg
    37501 caccgtcatt gacggaaggc tccagtaact gatcgcgggc gcagtctgtt ctattgtcac
    37561 cagggtagtg tatcgggtcg attccacaat cgtcagccga tattgtgcgt ttgccacctg
    37621 atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca attgtgaaaa
    37681 gttcatctcg cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct tcatttatgc
    37741 ccactcattt tttaacgctt gatgatgcag ctgcagccat tgcaaagcga tgaccgacgc
    37801 tgcgttgtcg attttcccct cttctaccca ctggtatgcc tgttcccggc ttaccacatg
    37861 aacgcgaata tcttcgtttt catcagccag accatgaata ccgcttgcgg tcgtggcgtc
    37921 cacttcgccc accataattg acgaacgctc actggtgccc cccgggcttg ccaggaaact
    37981 taacaccggt ttggtccgtt tgactatcag tcccgcctct tcaatcgctt cgcgacgggc
    38041 aacatcttcc acactttcac cctcttctat catcccggca accatctcca gtagccaggg
    38101 ggtttcactg gtgtcgtatg cggcaatccg aatctgctca atcagcacaa cttcatcgcg
    38161 cactgggtca aagggtagca agactgcggc gtgaccgcgc tcaaaaattt cccgccgtac
    38221 ctcatgactc atttgcccgt tgaatagacg atgacgaaat ctataaagat ctaatgaaaa
    38281 aaagccgcga taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc caaatgtaac
    38341 gggcaggttg tctggcttaa gcattgttaa tgtcctggca ctaatagtga attaaatgtg
    38401 aatttcagcg acgtttgact gccgtttgag cagtcatgtg ttaaattgag gcacattaac
    38461 gccctatggc acgtaacgcc aaccttttgc ggtagcggct tctgctagaa tccgcaataa
    38521 ttttacagtt tgatcgcgct aaatactgct tcaccacaag gaatgcaaat gaagaaattg
    38581 ctccccattc ttatcggcct gagcctttct gggttcagtt cgttgagcca ggccgagaac
    38641 ctaatgcaag tttatcagca agcacgcctt agtaacccgg aattgcgtaa gtctgccgcc
    38701 gatcgtgatg ctgcctttga aaaaattaat gaagcgcgca gtccattact gccacagcta
    38761 ggtttaggtg cagattacac ctatagcaac ggctaccgcg acgcgaacgg catcaactct
    38821 aacgcgacca gtgcgtccct gcagttaact caatccattt ttgatatgtc gaaatggcgt
    38881 gcgttaacgc tgcaggaaaa agcagcaggg attcaggacg tcacatatca gaccgatcag
    38941 caaaccttga tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc tattgacgtt
    39001 ctttcctata cacaggcaca aaaagaagcg atctaccgtc aattagatca aaccacccaa
    39061 cgttttaacg tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc gcagtacgat
    39121 accgtgctgg cgaacgaagt gaccgcacgt aataaccttg ataacgcggt agagcagctg
    39181 cgccagatca ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga aaactttaaa
    39241 accgacaaac cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg caacctgtcg
    39301 ctgttacagg cacgcttgag ccaggacctg gcgcgcgagc aaattcgcca ggcgcaggat
    39361 ggtcacttac cgacgctgga tttaacggct tctaccggga tttctgacac ctcttatagc
    39421 ggttcgaaaa cccgtggtgc cgctggtacc cagtatgacg acagcaatat gggccagaac
    39481 aaagtgggcc tgagcttctc gctgccgatt tatcagggcg gaatggttaa ctcgcaggtg
    39541 aaacaggccc agtacaactt tgttggtgcc agcgagcaac tggaaagcgc gcatcgtagc
    39601 gtcgtgcaaa ccgtacgttc ctccttcaac aacattaatg catctatcag tagcattaac
    39661 gcctacaaac aagccgtagt ttccgctcaa agctcattag acgcgatgga agcgggctac
    39721 tcggtcggta cgcgtaccat tgttgatgtg ttggatgcaa ccaccacgct gtacaacgct
    39781 aagcaagagc tggcaaatgc gcgttataac tacctgatta accagttgaa tattaagtca
    39841 gctctgggta cgttgaacga gcaggatctg ctggcactga acaatgcgct gagcaaaccg
    39901 gtttccacta atccggaaaa cgttgcaccg caaacgccgg aacagaatgc tattgctgat
    39961 ggttatgcgc ctgatagccc ggcaccagtc gttcagcaaa catccgcacg cactaccacc
    40021 agtaacggtc ataacccttt ccgtaactga cgacgacgac ggggcttcgg ccccgtctga
    40081 acgtaaggca acgtaaagat acgggttatc tgccgcattc ttcccccttc tcgcttcaat
    40141 ttcgaccagc catcctctat tctgatgggt atttaccact ggtcccggaa gacaaaaatg
    40201 aaacggacaa aatccatacg ccacgcatcg ttccgcaaaa actggagcgc acgccatctg
    40261 acaccagtcg ctctcgcggt tgccactgtt tttatgctgg ctggctgtga aaagagtgat
    40321 gaaacagtgt ctctctatca aaatgctgac gactgttcag ctgcaaaccc aggcaaaagc
    40381 gccgaatgta ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac tgcgccgaaa
    40441 tacgccaccc gtgaagactg tgttgctgaa tttggtgaag gtcagtgcca gcaggcacca
    40501 gcccaggctg gcatggcacc agaaaaccag gcgcaggccc agcaatccag cgggagtttc
    40561 tggatgccgc tgatggccgg ttacatgatg gggcgtctga tgggcggcgg cgcgggattt
    40621 gcacagcagc cgctgttctc ctcgaaaaac ccagccagcc cggcttacgg taaatatacc
    40681 gacgcgacgg gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac cgtaccgaag
    40741 acagccatgg caccaaaacc ggcgaccact actaccgtta cccgtggcgg ttttggtgaa
    40801 tctgttgcca aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc tcgttcaatg
    40861 ggtggctgat accgatggaa agagtcagta ttaccgagcg cccggactgg cgcgaaaaag
    40921 cccacgaata cggtttcaat tttcacacca tgtacggcga gccgtactgg tgtgaagatg
    40981 cttactacaa gttgaccctc gcccaggttg aaaagctgga agaagtcacc gccgaactgc
    41041 accagatgtg cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg atgaccaaat
    41101 tccgcattcc aaaacgcacc tggagttttg tgcgtcagtc atggctgact caccagccat
    41161 cgctttattc gcgtcttgat ctggcatggg atggcactgg cgaacctaag ctgctggaaa
    41221 ataacgccga tacaccaacg tcactatacg aggcggcgtt ctttcagtgg atctggctgg
    41281 aagatcagct taacgccggt aacttgccgg agggcagcga ccagtttaac agtctgcaag
    41341 aaaagctaat cgatcgcttt gtcgagttgc gtgaacagta tggcttccag ttgctgcatc
    41401 tcacctgctg tcgcgacacg gtggaagatc gcggaaccat tcagtatttg caggactgcg
    41461 cgacggaagc tgaaatcgct accgagttcc tctacattga cgatatcggg ttaggtgaaa
    41521 aaggtcagtt cacggattta caggatcagg taatttccaa cctgttcaag ctgtatccat
    41581 gggaatttat gttgcgtgag atgttctcca ccaagctgga ggatgcaggc gtacgctggc
    41641 tggaaccggc gtggaagagc attatctcca acaaagcgct tctaccgcta ctgtgggaga
    41701 tgttcccgaa tcacccgaac ctgctgcccg cttattttgc ggaagatgat catccgcaaa
    41761 tggaaaaata tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg tcgatcattg
    41821 agaacggcaa aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg atgattgttc
    41881 agcaattcca cccgttaccg aaattcggcg acagctatat gctgattggt agctggctgg
    41941 tgaacgatca acctgccgga attggcattc gtgaagaccg tgcgttgatc acccaggata
    42001 tgtctcggtt ttatccacat atttttattg agtaatcaat gatgccggat ggcattcgcc
    42061 atccggtaat tgttaaccta tctgcaccga cagcatactc aggctgccca tttctatacc
    42121 atcgaccgga atggtaattg gctcctgccc atcccacgca cctaagacat acaacaacgg
    42181 cagatagtgc tccggcgttg ggttcgataa cgcgccacct tcatggtcaa ggtaattcac
    42241 cagaggatgc tgctctaccg ccccttgcca cgtcagattc gctttcacat actcattaaa
    42301 cgacatcgcc caaggatatg gtgaactatc accgtgccac ttcactgtac gcaggttatg
    42361 caccacgttg ccactggcga ccaacattat tccttcatct cgcagcgctg ccagtttgcg
    42421 ccccatttcg aaatgccagg cggcaggttt gctactgtcg atactcaact gcaccatcgg
    42481 gatatcggcg tcaggataca tcttaatcag cacgccccag gaaccgtggt caaagcccca
    42541 ggcttcttta tccagcgtca ccgggaccga cgctaacagc tcaaccagat gctgtgccag
    42601 cacaggcgaa cccggagcag gataatgcgt atcgtacagc gcctgcggga agccaccaaa
    42661 gtcatgaatc gtgggcggcg tctccatcgc ggtcactcct gttccacggg taaaccagtg
    42721 agccgaaacc accacaatcg cttgcgggcg tggcaatgtc atccccaact tctgccagct
    42781 gcgggtatac aaattatctt ccagcacgtt catcggacta ccgtgaccta aaaacaatgc
    42841 tggcatacgt gttgaagaca tgatgatatc cttaactaaa ggtgtcattt tgatatcctc
    42901 acaatacgct tgttcggcgg agtaagaacc cggataacaa tgatgatgat catcagttat
    42961 tttgacgatc tgcctgaagg tgaagattta taaggagttg tcgatgtcag tacctctcat
    43021 tctgaccata ctggcggggg cagccacgtt tattggcgcg tttctcggcg ttctcgggca
    43081 aaaaccctcg aaccgcttac tggcgttttc gctaggattt gcggcgggga tcatgttgct
    43141 catctcatta atggaaatgc ttcctgccgc actagcagct gaaggaatgt cgcctgtgtt
    43201 gggttatgga atgtttatct tcggtctgct tggctatttt ggcctggacc gcatgttgcc
    43261 acatgcgcat ccgcaggatt taatgcaaaa atcggtgcag ccgttgccaa aatcgatcaa
    43321 gcgcacagcc attctgctca ctctcggcat cagtctgcat aacttcccgg aagggattgc
    43381 cacctttgtc acggcgagca gcaacctgga gctgggattt ggcatcgcac tggccgtcgc
    43441 gttgcacaat atccctgaag gtctggcagt ggcaggcccg gtttatgcgg caacggggtc
    43501 taaacgcacc gcgattctgt gggcggggat ttctggacta gcagaaattc ttggtggtgt
    43561 gctggcgtgg ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg caatcatggc
    43621 ggcggttgca ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc tcgccaaaga
    43681 gattgaccct aataataacc ccagctatgg cgtactatgt ggaatgtcag tgatgggatt
    43741 cagtttagtg ctgctacaaa cggcgggaat tggttaaaaa caacatcaga agttgtccag
    43801 cacatggaca acttctgcca gcttcacgaa agccttattc taccttttcg gctgtctctt
    43861 ctctcgtact gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg tcctggcgaa
    43921 acgcccgaca ataacgtttc aaatgcagga aataacccac cacaaccaga gcaatcaata
    43981 caatccagta ccaggcgata aacatccttt tacccttaat atttaacaat actaaacatt
    44041 tagcgcataa atttcacata tcctttttcg gatatattca tatggtcgca taataacgga
    44101 acaattatat gattttgatc ataatgaaaa agaatgcctg atattaatga aattttctca
    44161 tagaaagaga agcggaatta acatcttgca tagcagggca aaataaaaac cgaagccagc
    44221 tacaacttcg gtttcttgat taaggcagta attaagcagc ggttttcagc tggctttacg
    44281 ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca tattgtgttt
    44341 attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat tagtcagctc
    44401 acacagcaca ccagccggtt taaagcctgc cagcgtcatc aaatcaatag tcgcttcagt
    44461 atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa cgtggcccgg
    44521 gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg tcgtaatacg
    44581 atcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca cggtaaaacc
    44641 ggtgccatag gcgctggtgt tattttctac catcattggc agatcaagtt gtttacggcg
    44701 atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca gcgccatctg
    44761 ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac ggtcttcatc
    44821 atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt caacacgttc
    44881 gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaac ctcactaaaa
    44941 ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa aataacgtga
    45001 gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac cgtcggcccc
    45061 ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc gggctttcaa
    45121 ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag cggattcact
    45181 ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt ttatgactta
    45241 cccttatcgc actacaatgg tactcaacac ctatcagtac agggaaacta caatgattga
    45301 cccgaaaaaa attgagcaaa tcgctcgcca ggttcacgaa tcaatgccta aaggaatcag
    45361 ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc agctgacgcg
    45421 cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt tacggactcg
    45481 tgagaaactg gcgcggcttg aacaaagaat gagcgaactg gaaaatcgca gtactgagat
    45541 taaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg tctttaatgc
    45601 actgtaaatt atgaattaat cttctaattt acagtgcttt ctat
//
LOCUS       DAZPEP010000016        43677 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZPEP010000016 DAZPEP010000000
VERSION     DAZPEP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.016"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            236..1138
                     /locus_tag="ACHUT9_000898"
     CDS             236..1138
                     /locus_tag="ACHUT9_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1347446.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPVFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            1285..2505
                     /locus_tag="ACHUT9_000899"
     CDS             1285..2505
                     /locus_tag="ACHUT9_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1347447.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKNSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPV
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            2478..3107
                     /locus_tag="ACHUT9_000900"
     CDS             2478..3107
                     /locus_tag="ACHUT9_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFR1347448.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVICLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(3108..4268)
                     /gene="ybdL"
                     /locus_tag="ACHUT9_000901"
     CDS             complement(3108..4268)
                     /gene="ybdL"
                     /locus_tag="ACHUT9_000901"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFR1347449.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            4377..5465
                     /gene="hcxA"
                     /locus_tag="ACHUT9_000902"
     CDS             4377..5465
                     /gene="hcxA"
                     /locus_tag="ACHUT9_000902"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFR1347450.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(5475..5672)
                     /locus_tag="ACHUT9_000903"
     CDS             complement(5475..5672)
                     /locus_tag="ACHUT9_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1347451.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(5854..7959)
                     /gene="cstA"
                     /locus_tag="ACHUT9_000904"
     CDS             complement(5854..7959)
                     /gene="cstA"
                     /locus_tag="ACHUT9_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFR1347452.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(8140..8553)
                     /gene="entH"
                     /locus_tag="ACHUT9_000905"
     CDS             complement(8140..8553)
                     /gene="entH"
                     /locus_tag="ACHUT9_000905"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFR1347453.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(8556..9302)
                     /gene="entA"
                     /locus_tag="ACHUT9_000906"
     CDS             complement(8556..9302)
                     /gene="entA"
                     /locus_tag="ACHUT9_000906"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFR1347454.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(9302..10159)
                     /gene="entB"
                     /locus_tag="ACHUT9_000907"
     CDS             complement(9302..10159)
                     /gene="entB"
                     /locus_tag="ACHUT9_000907"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFR1347455.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(10173..11783)
                     /gene="entE"
                     /locus_tag="ACHUT9_000908"
     CDS             complement(10173..11783)
                     /gene="entE"
                     /locus_tag="ACHUT9_000908"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFR1347456.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(11793..12968)
                     /gene="entC"
                     /locus_tag="ACHUT9_000909"
     CDS             complement(11793..12968)
                     /gene="entC"
                     /locus_tag="ACHUT9_000909"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFR1347457.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            13343..14308
                     /gene="fepB"
                     /locus_tag="ACHUT9_000910"
     CDS             13343..14308
                     /gene="fepB"
                     /locus_tag="ACHUT9_000910"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1347458.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(14389..15639)
                     /gene="entS"
                     /locus_tag="ACHUT9_000911"
     CDS             complement(14389..15639)
                     /gene="entS"
                     /locus_tag="ACHUT9_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFR1347459.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            15750..16754
                     /gene="fepD"
                     /locus_tag="ACHUT9_000912"
     CDS             15750..16754
                     /gene="fepD"
                     /locus_tag="ACHUT9_000912"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFR1347460.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            16751..17743
                     /gene="fepG"
                     /locus_tag="ACHUT9_000913"
     CDS             16751..17743
                     /gene="fepG"
                     /locus_tag="ACHUT9_000913"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFR1347461.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            17740..18555
                     /gene="fepC"
                     /locus_tag="ACHUT9_000914"
     CDS             17740..18555
                     /gene="fepC"
                     /locus_tag="ACHUT9_000914"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1347462.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(18552..19685)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUT9_000915"
     CDS             complement(18552..19685)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHUT9_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFR1347463.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(19901..23782)
                     /gene="entF"
                     /locus_tag="ACHUT9_000916"
     CDS             complement(19901..23782)
                     /gene="entF"
                     /locus_tag="ACHUT9_000916"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFR1347464.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     TDLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(23779..23997)
                     /gene="ybdZ"
                     /locus_tag="ACHUT9_000917"
     CDS             complement(23779..23997)
                     /gene="ybdZ"
                     /locus_tag="ACHUT9_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFR1347465.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(24000..25202)
                     /gene="fes"
                     /locus_tag="ACHUT9_000918"
     CDS             complement(24000..25202)
                     /gene="fes"
                     /locus_tag="ACHUT9_000918"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFR1347466.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            25445..27685
                     /gene="fepA"
                     /locus_tag="ACHUT9_000919"
     CDS             25445..27685
                     /gene="fepA"
                     /locus_tag="ACHUT9_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFR1347467.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            27860..28480
                     /gene="entD"
                     /locus_tag="ACHUT9_000920"
     CDS             27860..28480
                     /gene="entD"
                     /locus_tag="ACHUT9_000920"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFR1347468.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(28602..28754)
                     /gene="hokE"
                     /locus_tag="ACHUT9_000921"
     CDS             complement(28602..28754)
                     /gene="hokE"
                     /locus_tag="ACHUT9_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1347469.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(29105..29257)
                     /gene="hokE"
                     /locus_tag="ACHUT9_000922"
     CDS             complement(29105..29257)
                     /gene="hokE"
                     /locus_tag="ACHUT9_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFR1347470.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            29226..29494
                     /locus_tag="ACHUT9_000923"
                     /pseudo
     CDS             29226..29494
                     /locus_tag="ACHUT9_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            29697..30815
                     /locus_tag="ACHUT9_000924"
     CDS             29697..30815
                     /locus_tag="ACHUT9_000924"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFR1347471.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            30881..31129
                     /locus_tag="ACHUT9_000925"
                     /pseudo
     CDS             30881..31129
                     /locus_tag="ACHUT9_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            31194..31562
                     /locus_tag="ACHUT9_000926"
     CDS             31194..31562
                     /locus_tag="ACHUT9_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1347472.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            31656..32309
                     /gene="nfsB"
                     /locus_tag="ACHUT9_000927"
     CDS             31656..32309
                     /gene="nfsB"
                     /locus_tag="ACHUT9_000927"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFR1347473.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            32417..33664
                     /gene="ybdG"
                     /locus_tag="ACHUT9_000928"
     CDS             32417..33664
                     /gene="ybdG"
                     /locus_tag="ACHUT9_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFR1347474.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(33732..35108)
                     /gene="pheP"
                     /locus_tag="ACHUT9_000929"
     CDS             complement(33732..35108)
                     /gene="pheP"
                     /locus_tag="ACHUT9_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFR1347475.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(35210..38353)
                     /gene="cusA"
                     /locus_tag="ACHUT9_000930"
     CDS             complement(35210..38353)
                     /gene="cusA"
                     /locus_tag="ACHUT9_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFR1347476.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPSVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(38365..39588)
                     /gene="cusB"
                     /locus_tag="ACHUT9_000931"
     CDS             complement(38365..39588)
                     /gene="cusB"
                     /locus_tag="ACHUT9_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFR1347477.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(39604..39936)
                     /gene="cusF"
                     /locus_tag="ACHUT9_000932"
     CDS             complement(39604..39936)
                     /gene="cusF"
                     /locus_tag="ACHUT9_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFR1347478.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(39960..41342)
                     /gene="cusC"
                     /locus_tag="ACHUT9_000933"
     CDS             complement(39960..41342)
                     /gene="cusC"
                     /locus_tag="ACHUT9_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFR1347479.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            41499..42182
                     /gene="cusR"
                     /locus_tag="ACHUT9_000934"
     CDS             41499..42182
                     /gene="cusR"
                     /locus_tag="ACHUT9_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFR1347480.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            42172..43620
                     /gene="cusS"
                     /locus_tag="ACHUT9_000935"
     CDS             42172..43620
                     /gene="cusS"
                     /locus_tag="ACHUT9_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFR1347481.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10588 a        12002 c        11039 g        10048 t
ORIGIN      
        1 catcacccat tatgggaaat aaataaaagg taattcatgg atggttgaac tatatccctg
       61 cttttcaacc atcctaaaaa agcatgaatt gctcgctcta ccctaagtgg ctgtgctatt
      121 ttagaaaaac ttaatcctga gttaagcaaa aaaacataat caataaaaat acatacgcaa
      181 aaaacattga ttaagtgaat ataccatgga agaaaaatat aaaccggagt agtgtatggc
      241 caatctctac gacttgaaaa aattcgactt aaatcttctt gtcatatttg agtgtattta
      301 ccagcattta agcattagca aagctgctga atcactatat atcaccccat cagccgtgag
      361 ccagtcgtta caacgcttac gtgcgcaatt taatgaccct gtattcattc gctcaggaaa
      421 aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga aaaacctcaa
      481 aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa actttattat
      541 ctacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct gcctacggca
      601 agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga atgctgaaga
      661 actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga tcagccgttc
      721 cgtcatttgc atgccattgc atacaattcg caatactcta atttgtagta ataagcatcc
      781 gcgaattact gacaatagca cttatgaaca aattatggca gaagagttca cccaattaat
      841 atccaaatca gcaggagttg atgatattca aatggaaata gacgagaagt ttatgaatcg
      901 caaaatttca tttcgtggtt cctctttgct gacaataatt aatagcattg cagttacaga
      961 tttactggga atagtcccct atgaacttta taactcccac cgtgactttc ttaatttaaa
     1021 agagataaaa ctagaacatc cgttaccttc aataaagctt tacatttcct ataataaatc
     1081 ttctttaaat aatttggtgt tctcccgatt tattgaccgt ctaaatgaga gtttttgata
     1141 tataactaca gacagctact ataactttca tctatttatt cacagcgcga atgaaaaata
     1201 aaacactctg ctttacagtg tttactttat aattaacagt cttatctcac tgttcaccct
     1261 gtaacgttca gagagcgtct tcagatgtct atttataaaa ttcctcttcc gctcaatatt
     1321 ctggaagctg cacgagagcg tattacctgg acactcaaca ccctaccccg cgtttgtgtc
     1381 tcattttctg gcggcaaaaa ctctggttta atgctgcatc tgacagccga acttgcccga
     1441 caaatgggca aaaaaatctg cgttttgttt atcgactggg aggcgcaatt ctcttgcacc
     1501 attaactatg ttcagtccct gcgcgagttg tacaccgatg tcatcgaaga gttttactgg
     1561 gttgcgctcc cgcttacgac gcaaaattcc ctttcacaat accaacccga atggcagtgc
     1621 tgggaacctg atgtcgaatg ggtgcgtcag cccccgcaag atgcgataac cgatcctgac
     1681 tttttctgct tttaccagcc aggcatgacc ttcgaacaat ttgtacgtga gtttgccgaa
     1741 tggttttcac aaaaacgtcc ggtggcgatg atgatcggca tccgtgcgga tgagtcctac
     1801 aaccgttttg tcgccatcgc cagtttaaat aaacaacgtt ttgccgacga taaaccatgg
     1861 actacggccg caccaggcgg tcatagctgg tacatttacc ccatttacga ctggaaagtg
     1921 gctgatatct ggacctggta tgcaaatcat caaaatctct gcaacccact gtataaccta
     1981 atgtatcagg caggcgttcc tctgcgtcat atgcgaattt gcgaaccttt tggcccggag
     2041 caacgacaag gattatggct ctatcacgtt atcgaaccgg atcgctgggc tgctatgtgc
     2101 gcacgagtca gcggggtaaa aagtggcggc atttacgccg gacatgataa tcatttctat
     2161 gggcatcgga aaatcctcaa gccagaacat ttagactggc aagaatatgc attattgctg
     2221 ctcaatagca tgccggaaaa aacagctgag cattaccgca ataaaatcgc catttatttg
     2281 cactggtatc agaaaaaagg catcgaggtt ccacaaaccc agcaagggga cattggcgcg
     2341 aaagatatcc cctcctggcg gcggatatgc aaagttttac tcaataacga ttactggtgt
     2401 cgggcattat catttagtcc tacgaaagcg aagaactatc agcgttataa cgaacggata
     2461 aaaggaaaac gtcaggaatg ggggatacta tgcaacaacg attaacgcag gatttaacgc
     2521 aatttctcgc cagcttgccg gaagatgatc gcatcaaagc gattaatgaa atccgcatgg
     2581 cgatccatca ggttagcccc tttcgtgaag agccagtgga ttgcgttctg tgggttaaaa
     2641 acagccaact tatgcctaat gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac
     2701 tgctgcaaaa atccattgaa atagatggtt ttacccaacc cattgtggtc acacatacgg
     2761 ataaaaatgc catggaaatc gtggatggtt ttcaccgcca tgaaattggt aaaggctcat
     2821 catcattaaa attacgctta aaaggttatt tgccggtgat ctgtctggaa ggtacgcgta
     2881 atcaacgcat cgccgcgact attcgtcata atcgcgctcg tggacgccat caaatcaccg
     2941 ccatgtcgga aatcgtccgt gaacttagcc agttgggatg ggacgataat aaaattggca
     3001 aagagctggg aatggacagc gacgaagtgc tgcgcctgaa gcaaattaac ggcctgcaag
     3061 agctgtttgc cgaccgtcaa tattcccgcg cctggacggt taaataacta aagctggcgc
     3121 aggcgttcag ctgctgccag caacgtcgat tccttcttgg caaaacagag acgaatcagt
     3181 ttatgtggga agggatcggc gcaaaacacc gacagcggaa tcgccgctac gccgtgctcc
     3241 tgcgtcagcc actggcaaaa ctcaacatca tccagggtag aaaccgcgct gtaatccacc
     3301 agcaaaaagt atgtaccttc acacggtaaa atctccagcc ggctttcatt taaggcattt
     3361 accagaatat cgcgcttctg gcgataaaag tccggtaacg caagataatg ctcaggttct
     3421 gcacgtagca tatcagcaag cgccagctgt gccggggtat tcaccgaaaa ggtcagatac
     3481 tgatgtacct tgcgaatttc ggcgctgatg ggcgctggcg caacacaata acccactttc
     3541 cagccggtca tatgataggt cttgccaaat gaagaaaccg ccactgcccg ctcacgcagc
     3601 tgcgaatgcg ccagcacact ggcatggccc tgttgtgaaa agttgatgtg ctcgtagact
     3661 tcatcgctaa tgacaaaaat ctcgtgcccg gcgatcgcct gccacaaagc ggcgaaatca
     3721 gcctgctgcc agacagttgc actggagtta tgcggagtgt tgaggatcac cagtctggtg
     3781 cgctcgctta ataatgcggc aaattcctgc cagtcaacgc gaaaatgcgg tggttgcagt
     3841 gccatacgct tcactattcc cccagaaagc gcgatggcgg gggcgtaact gtcatagctg
     3901 ggatcaaaac aaatcacttc atcgccattg cgcaccagtg cggtaatcgc cgcgtataac
     3961 gcttccgtcg cccctgccgt tacggtgata tcgctatcgg catctggttg atagccatac
     4021 aaacgttccg ttttctgagc aatcgcctcg cgcaaggcct gcacgccggt catgggcgcg
     4081 tattggtttg ccccctgggc aacgtggtac gccagccgct cctgtaaata gcgcggacca
     4141 tcaaaatcag gaaagccttg cgacaggtta atcgcctggt gttgctgcgc cagcgcgctc
     4201 atctgggtga aaatagtggt gccaagttgt ggaagtttgc tttgtggaat cagagggtta
     4261 tttgtcattt ttattgtacc tgacgaagtt gcgtgttgaa ggcactataa cacgatgcta
     4321 gtatttggca atcaagacgt ttagatgtct aaataaaaca ataaggacaa cacaacatgc
     4381 ctcacaatcc tatccgcgtg gtcgtcggcc cggctaacta cttttcacat ccaggaagtt
     4441 tcaatcacct gaacgatttt ttcactgatg aacaactttc tcgcgcggtg tggatctacg
     4501 gcaaacgcgc cattgctgcg gcgcaaacca aacttccgcc agcgtttgga cttccagggg
     4561 caaagcatat tttgtttcgt ggtcattgca gcgaaagcga tgtacagcaa ctggcggctg
     4621 agtccggtga cgatcgcagc gtggtgattg gcgtcggcgg aggcgcactg ctcgacaccg
     4681 cgaaagccct cgcccgccgt ctcggtctgc catttgttgc cgttccgacg atcgccgcta
     4741 cctgcgccgc ctggacaccg ctctccgtct ggtataacga tgccggacag gcgctgcatt
     4801 atgagatttt cgacgacgcc aattttatgg tgctggtgga accagagatt atcctcaacg
     4861 cgccgcaaca atatctgctg gcggggatcg gtgacacgct ggcgaaatgg tatgaagcgg
     4921 tggtgctggc tccgcaacca gaaacgttgc cgctaaccgt gcgactgggg atcaataatg
     4981 cgcaggccat tcgcgacgta ttgttaaaca gtagcgaaca ggcgctgagc gatcagcaaa
     5041 atcaacagtt aacgcaatca ttttgcgatg tggtggatgc tattattgct ggtggtggga
     5101 tggtcggtgg tctgggcgat cgttttacgc gtgtggcggc ggctcatgcc gtgcataacg
     5161 gtctgactgt cctgccgcaa accgagaaat ttctccacgg caccaaagtc gcttatggca
     5221 ttctggtaca aagcgccttg ctaggtcagg atgatgtgct ggcgcaatta accggggcgt
     5281 atcagcgttt tcatctgccg accacgctgg cggagctgga aatggatatc aataatcatg
     5341 cggagatcga caaagtgatt acccacaccc tgcgtccggt ggaatacatt cattacctgc
     5401 cagtcacgct gacaccagat acgttgcgtg cagcgttcga aaaagtggaa tcgtttaaag
     5461 cctgacgtgc aaatttagca gcagcgcgcg ccgccttttc caccgtagcg cgcgtcctgc
     5521 cgctcacgga aaaactcttc gtaggtcatc ggcgtttgat cgggatggtt aacccgcata
     5581 tgttcgacat agttgtcgta atcaggcata ccaatcatca gcttcgccgc ctgacctaaa
     5641 tattttccgg cttttgccag tgaatcaaac atcttggcct ccgctaacca tgttgctgaa
     5701 agagtgccgg atgcggcgtg aacgccttat ccggcctaca aatgctatca acgcgctcgg
     5761 tcagtcccct cgccccatcg gggagagggt tagggtgagg aggaaccctc ggcactatgc
     5821 caacattcgc caacatcccc cctcactctg actttagtgt gcgccttttg cctgcgccac
     5881 gatctcctcg acattttccg gcattggctc atacggcgtt tctttcgccg ttggcttcgg
     5941 atctttcaat gccgccagtg ccgacttaat cgagaacagc gccagaacca ccacgaccac
     6001 cataaagaag atggttaacc cggcatccag acggttgttg aacaccagtt gcgccagctg
     6061 tgactcagta tactgcgacg gaatattgcc gctgtcgatc attgcctgga acttattagc
     6121 aatggccagg aagccgactt tcgcatccgg gctaaacgct ttctgccagc ctgcggtcag
     6181 ggtacaaatc agcagccagg ccgttggtac cagcgccacc caggcgtaac gttgacgttt
     6241 catcttgaac aacaccacgg cacagagcat cagcgccatc cctgccagca tctggttggc
     6301 aataccaaac agcggccaca gagtgttaat gccgcccagc ggatcgacca cgccctgatg
     6361 gaggaagtag ccccacgcca gtacgcacag cgctgttgcc agcaggttag caggcagtga
     6421 atcggtccgc ttcaggccag gagacaccac gcccagcaga tcctgcaaca taaagcgcgc
     6481 agcacgcgta cccgcatcta ccgccgtcag aataaacaga gcttcaaaca gaatggcgaa
     6541 gtgataccag aacgccacat ccatcatacc acccagcgca ccgtgcagga tgtaagccat
     6601 ccccaccgcc agcgtcggcg caccgcccgc acgggaaatg atcgactgtt cacccacttc
     6661 gctggcaatc tggttgagcg tatccggagt aatcgcaaag ccccagctac tcaccacctg
     6721 cgcggcagaa gcgaccacat ccgccgtccc tgccggagcc agcaccgcca tcgggctgtt
     6781 catggcgaag tacacgcccg gatcgatgat acaggcggaa accagtgcca taatcgccac
     6841 gaaggattcc attaacattc caccgtagcc gataaagcac gcctgccctt cgttcgccag
     6901 catcttcggc gtggtgccgg aagagatcag cgcatggaag cctgacaccg cgccacaggc
     6961 gatggtgata aacaggaacg ggaacagatt accggtccat accgggccag tgccatcgac
     7021 aaatttggtc agcgcaggca tggtcagcgt cggacgcata atcaaaatgc ctaccgccag
     7081 accaacgata gtcccgattt tcaggaaggt agagaggtag tcacgcgggg ccagcagtaa
     7141 ccacaccggc agcaccgccg ccacaaaacc gtagcccacc agcatccagg tcagctgcac
     7201 gccggtaaag tcaaagtacg gtgcccaggt cgggctttct gccacccagc cgccagagat
     7261 aatggcaaaa ataaggaaca ccaggccaat cactgacact tcgccaatac gccccggacg
     7321 cagatagcgc aggtagattc ccataaaaat cgccagcgga atggtgaacg caacggtata
     7381 cgttccccac gggctatgag tcagggcttt caccacgatc atcgccagta ctgcaaggat
     7441 aatgaccatg atcataaagc aggctaccag cgcaatcacc ccggcagttg gccccatctc
     7501 ttctttgacc agctcaccca gcgagcgacc gtcacggcgc gtagaaacaa acagcaccat
     7561 gaaatcctgc accgcaccgg cgagaaccac cccggcgagc agccagatca tccccggcag
     7621 gtagcccatt tgcgctgcca gcaccggccc caccagcggc cctgctccgg caatggccgc
     7681 aaaatggtga ccgaacagca ctttcttgtc cgtcggcaca tagtccagcc cgtcgttatg
     7741 gcgcaccgct ggcgtcatac gcgtcgggtc aaccgccagc acatttttgg cgatatacag
     7801 cccataaaaa cggtaagcga tcagatagat acagaccgac gccaccacaa tccacagcgc
     7861 gttgatctgt tccccacgat ttaaagcaat gtatcccaga gcaaatgctc ccattacaga
     7921 gagcactgtc cagacgaggt atttccctga tttgttcata gttgttatcc gtgtgcgtgt
     7981 ccatagagat gttacatttt gtttctataa catctttgta cgatttaaca acacggtaac
     8041 catgtaaatc ggagttgaaa ccaatattta acttaacaat gttaactcga tcactccgtt
     8101 aaccgagttt tcctgcaatc tcatttaatt ctgtcgggct catcccaaaa ctgccgtacc
     8161 cagccgacaa gtgcagcaac gccgcccctg ttcatcgaaa acgatgattt cccagctctg
     8221 attttgccgc ccaagatgca gcggctggca gacgccgcgt accttgcctt cagacaccgg
     8281 gcgatgatgg gtggcgttaa gttccgtacc caccacacac tgcccgtcac gggtcatcat
     8341 aaatccggcc atcgatccca gcgtttcagc cagcgccgcc gacgcgccgc catgcagcag
     8401 gccaaacggc tgatgagtac gggtatcaac cggcatttcg gcttccagca catcatctcc
     8461 cagacgggta tacacaattc ccagatgcgc caccattgtg ttatcgctgg tggcgttcag
     8521 ttcgtcgagc gttaaatggc gtttccagat catgcttatg cccccagcgt tgagccgcca
     8581 tcgaccacaa tatcctgtag ggtgatatgg ctggcgaggt cagaggcgag gaacaaaatc
     8641 gtgttggcga tctcttgtgg acgggcgatt ttccccagcg gaatgccgag tttaaactgc
     8701 tcgccaaagc cgcgaatacg ctgttcttcg gcgtcatcgc tcacccacag cgtgcgttgc
     8761 atatcggtgt cggtggagcc aggcgaaacc acattacagc gcacgccgct aaccgccagt
     8821 tccagcccga cgctcaacgc caggcttttc agcgccgctt tcgatgcgcc ataagcactc
     8881 ataccaatac gcggcgtgtg tgcggcgtcg gacgccacag tgacaatcgc cccgccccgc
     8941 tgacggcgaa actggttcat ggtttgctga aacaggttaa acgcaccgcc gacgttaacc
     9001 gcaaaagtct gctgccagtc ctctttactg agctgatcgg tcgcgcccat gcgcaaaatt
     9061 cccgccgcat tgaccagcac gtccagtcgc tccgtttcag ctaacagtcg ctgacatacc
     9121 tgcgcgacct gcgcagcatc ggcaacatcc atcacttcgg tcgcaaaggg atattgctcc
     9181 tgagcgaacg cttgatcaaa accggtaact ttcgctcccg cttcaacaaa cgccagcgcc
     9241 gtggcgtagc cgataccttt acctgcgccg gttacccaga catttttacc gctgaaatcc
     9301 attatttcac ctcgcgggag agtagcttcc accaggcgtc gatggtcggg tttttcgcca
     9361 gcatgacaaa gtcgatatcg ccatgcactt tgcgccagcg cgccgccagc gccatcatgc
     9421 gcaccgaatc cagaccgtag tcgatcaggt tgtcgtcatc gaacggttca tcggactcgt
     9481 ccagcaacgg caggataacc tcacgcagcg ccgctttcct ggcggggaca ggtgctggca
     9541 gtaatccttc agtcatcacc acccggccag accgcccggc cacatatttc agcgacatca
     9601 aatgctcgtc acggctgaaa tcggccagcg catccgccac cataaacggt ttaatatcgc
     9661 gcataaatgc gtcggttgcg gtggtcatac agccaatgtg ggcatatacc ccggtaataa
     9721 tcagctggtt acgtccactc tctttgagca tttgctccag cggagaacga tgaaacgcgc
     9781 tgtagcgcca cttcaccagc accgtgtcgt cggcatctgg cgtcaggcga tccaccacct
     9841 tttgctgttc cggcgaacgg gtcaggcccg gcccccacat atcattcaac agcgcccgat
     9901 cttcatcgct ctgctctttc ggctgggcgg tgtaataaac cgggatattg tgctgtttgc
     9961 agtagtcgcg cagcgcagca atattcgcga tcacctgctc catcatcggg cagttctcgc
    10021 cccagaagct gacaaaatag tcctgcatat catggattaa caacgcggca cgttgcggtt
    10081 caaaggccca gtcaacttta ttctgcggaa tatcgtgaga ctccggcagt gcgtaagcct
    10141 gtaattttgg aatagccatc gtgttctctc cttcaggctg atgcgcgtga cgccagccac
    10201 tgacgtaatt gttttttatc gactttcccg accgccgtca gcggaagtga atccacacac
    10261 tccacgcgat ccggtaattt aaattcggca ataccctgtt cacgcaggaa acgacgcacc
    10321 tgcaccgcgc gcagcggctc ttttaccacc agataagcac agcttttttc gcccatcagc
    10381 tcatcttcca tgctcaccag tgcggcatag atcaccgccg gatggcgcag cagcaggttt
    10441 tcgatctctt cggcagcgat cttctcgccg ccacggttga tctgatcttt ctcgcgcccc
    10501 tgcacggtga tgtaaccctc tggatcaata gagatcagat cgccggaaca gtaaaaaccg
    10561 ttggcatcaa aggcactggc attgtgctgc ggacttttgt aataaccgcg gaaggtgtac
    10621 ggcccgcgcg tcatcaggcg tccaacttcc ccttgcggca gtggatttcc ttcagcatca
    10681 gcaacccata cttcatcgtc cggacacatt gggtaaccct gggtatggat aattttctcc
    10741 gcgctatcat caagtcgggt gtagttcacc agcccttccg ccatgccaaa cacctgctgc
    10801 aactggcagc caatctcagc ggaaatacgc gccgcaaggg tggcagaaag acgtgcgccg
    10861 ccgacctgta acagtttcag cgaggcaagc tgcgcccggc tttcgccttc agccagcgcc
    10921 tgcaaccaca ggctgactgc cggcggcacc agcgcggtaa cgttaacctg atgttttgca
    10981 atcaacggga agcaaagcgt ggcgctggga tcggccgcca gaacaaccgt tcctccggca
    11041 agaaagacgc ccagcgatcc tggcgaactc atagcgtagt tatgagccgc cgggatcgcg
    11101 cacaggtagc gtgtctgttg tgtgaactga caaatctcga cgctacgacg cacgctgtag
    11161 tagtagtcgt tatgagtgcg cgggatcagc ttcggtgtac cggtggtgcc gccggaaagc
    11221 tggaaatagg ccacttcatc agcaggtgat ggcgtagcag taaaaccgtc tgccggatgg
    11281 ttaatcgcat cctgcaagtt atgctcaccg ctgtcgttga gcagttgcac cacgcgaatg
    11341 gaggaatgtt ctgtgacgaa agtattgagg aaatcatccc cgctaaacag cgcatgttgg
    11401 cgatcggcaa tcagtaacgc cggttcaatc tggctggcat aggcgttcag ttcactacgc
    11461 tgatggctga acaacgccag caccggcgca acgcccagtt tcagcagtgc gaaaaaggta
    11521 atgtaaagtt cagcgacgtt acccagttgt accagcgcgg tttcaccggg tttaatgccc
    11581 tgacggcgta aactacacgc gaggttatcc gccgcctgat tcagctcccg gtaactcaac
    11641 tgtcgctcgc cgtcgataac cgcgatgctg tcactcgcag cgtggcgagt cagaatgtcg
    11701 gtcagcggca aatcctgcca gtagcctttt tcccgatagc gacgggcaaa ctcttccggc
    11761 cagcgggtga atggaatgct catcctcgct ccttaatgca atccaaaaac gttcaacatg
    11821 gtagaaagtt tgacgcctgt ttcgcgccac tcacccaacg gtgacgacgc aggcacaatc
    11881 cccgctccgg caaacagacg cacctgattt tcccgcagct tcgcgcagcg gatggtcacc
    11941 acccattcgc cgttaccttc gctgtcacac caacccacaa tgccgccaaa cagttcgcgg
    12001 tcaaacggct ccagttcagc aataacctgg gtcgcggcct gatgcgggaa accgctcagc
    12061 gcgggggtcg gatgcagcag acaggccaga gtcagtgcgt tttcttgcga attcgcttta
    12121 ccttcaaagg gagttgcgag atgccacagc gtcggcgtgg taatcaattg tggagaggag
    12181 ggaacgtgta actcactact gcgttcgcgc agtacctctt tcatcgcctg agtcaccagt
    12241 tcatgttcat ggcgatcttt ttctgacgcc agcagacgat tacccgcttc gcgatcgagc
    12301 acttcatccg gctgacgacg cgcggaaccg gctaacggaa tggagctaaa acgctcgccg
    12361 tctttacgta gcagcagttc cgggctggcc cccagcagga cgccaccatc agccagcggg
    12421 acatggaagt tgtaactaac cgggttttgc gcaatcaacc gttccagcaa tacgccacta
    12481 tcaatggcgg cgtcagtggt gatatcaatc aatcgtgaca acaccacttt gtcgacctgc
    12541 ggcgtggcgg taagtgcggc ggcgcgggca accatctgtt caaacgtggt ttgttcagga
    12601 attgcctggc gttccaccac gttcagcgac tggctgcggg taaaacagtg ggctgaggtc
    12661 tgtttttcct gacgggagaa cgactgccag gattcgggaa tatacagcga cgaaggctga
    12721 cgtggatcga agggaatcgc cccgaccatc accggatttt tgatgccctg cgctttggca
    12781 tcggcaaaca gcgcggcgag tttttgctgg aagggactgt cgggcgaatc cccgttcaca
    12841 gccggttcat cgaagcgggc gaaacatcct gacgtcgtaa aactgcggta cggcgacata
    12901 aagaaaaagc gattgggcgc aagtgttgcc atggtctgct gtacttcctc agccagtgac
    12961 gtatccatat catcctccac aaaatgataa aggctttaat aatgattatc atttatattt
    13021 tcggtcgcta acctaaaagc aaacgcgcac tatgtcaact cttgaggtaa cgcaatctgc
    13081 aactcggggt tgcatctggt tgtggttaca atgaaaatga gaagcattat tgatgaattc
    13141 gcataagcgc aatgtgatgt cctgcgccgt tttgccccct ctcccttcca gggtgagggc
    13201 tggggtgagg gttaatgttc gcaccagtgc tggctgttcc cctcacccta accctctccc
    13261 caaaggggcg aggggacgga ttgtgcgctt tgtcgaattt gtcattacgc ccttaacctt
    13321 attaataaca ggaagctgac ttgtgagact cgccccgctc taccgcaacg cccttctatt
    13381 aacaggactt ttgctttcag gaatagccgt agttcaggcc gccgactggc cgcgtcagat
    13441 tactgacagc cggggcactc ataccctgga aagccagccg cagcgtattg tttccaccag
    13501 cgtcaccctg accggatcac tgctggcgat tgatgctccg gtgatcgcca gcggcgcgac
    13561 cacgccgaat aaccgcgtcg cagatgacca gggcttttta cgccagtgga gcaaggtggc
    13621 gaaagaacgc aaactgcaac ggctctatat cggcgaaccg agcgccgaag ccgttgccgc
    13681 gcaaatgccg gatctgattt taattagcgc aaccggcggg gattcggcgc tggcgctgta
    13741 tgatcagctt tccaccatcg ccccgacatt aatcatcaat tacgacgaca aaagctggca
    13801 gtcgctgtta acgcagcttg gcgaaattac cgggcatgag aaacaagcgg cagagcggat
    13861 tgcacagttt gataagcaac tggcagcggc gaaagagcaa atcaaattac cgccgcagcc
    13921 ggtcactgcc attgtctata ccgccgctgc acacagtgcc aatctctgga cgccggaatc
    13981 agcacaaggg cagatgctgg aacaactcgg ctttacgctg gcgaagttgc ccgcagggtt
    14041 aaacgccagc caaagccagg gcaaacgcca tgacatcatt cagcttggtg gggaaaatct
    14101 ggctgctggg ttaaacggtg aatcgctatt cctgttcgcc ggtgatcaga aagacgccga
    14161 tgcgatttat gccaatccac tgctcgcgca cctgcctgca gtacaaaaca agcaggttta
    14221 tacgctggga accgagacgt tccgcctgga ttactacagc gccatgcaag tgctggacag
    14281 acttaattca ctgttaaaaa aagattgata aaacatacaa ttagagcact aataatcccc
    14341 tcgccccttt ggggagaggg ttagggtgag gggaaaatgc acctttagct aactgtcaga
    14401 cgctgtcacc tgcggcggcg tctggcgaaa acgtcgcaac tccaccagca ccagcaataa
    14461 caacacgccg ataatcaaca aaccaaaacc gctcgcgctt gcggaagcaa ccggtgtcat
    14521 catcgcgccc aaaccaccca acagcgccgc gcctatggca tcgcccgtca cgttctgcgc
    14581 cgtccacaaa ccgttaatcc gccctaacat cgcttccggg gtttgcgttt gcagcattgt
    14641 atattgcagc aacgaactaa ccgcgctcag ccagccgaac agcgccagac aaaccacgcc
    14701 taaaatccac atcggcatca ggccaaacag accaatggcg aggaacgatc ccagcgtgga
    14761 gagcagcatc aataaccctg gtcgcgcact atgtgccagc ttcccgctgg ttaacgcacc
    14821 aatagccgca ccgagcggga tcgccgcata gagaaaacca atctgtgccg ccgacatctg
    14881 ccagttgtca gccagcgccg gatacagtac ccgcaccgcg ctcgccatcg tcaataaacc
    14941 acccagcaac gcaatccctc ctaccagcgg gctggcgagc agaaaacgaa atcctgccag
    15001 taatgatttc aacggatgct cgcgcggctg cggtggcggt ggcaacgccg gaaggcttaa
    15061 caacggtagc aaggtaataa acgtgcccgc cgccgccagc ccgtagttcc aggctacgcc
    15121 gccggtcgcc agcaataaac cgccaatcat gggcgaaatc accgacccca gacgcacggt
    15181 caacatggtg atcgccccgg cctgcattaa gttttcacgc cctaccagtg caggtgttgc
    15241 cgccagtagc gccgtaacac caagtgatgc gaaaaaacca tcccataaac caagtaaata
    15301 gattgccagc aatgacggct ccggcagcag cgcgttcagg cacagtccaa tgaagccaat
    15361 gccacaggtg ccgcgcgcca gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac
    15421 accgccaacc atcaggccaa caaacatcgc gccgccggtc agcgtcaccg aaagccccac
    15481 ctgccaggta gaatgcgtca tcatctggat ctgcaccggc accgcgacgc cgagcaaacc
    15541 cagagacaca attgagataa aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt
    15601 caacaggctg aggttaagca gccaggattg tttattcatt acaatgcctt gccatcaaat
    15661 attttaaata cctattctta ggctgcacat gctaacatat ccaaataaga tcgataacga
    15721 taattaattt cattatcatg gaagttcgta tgtctggttc tgttgccgtg acacgcgcca
    15781 ttgccgtgcc cggattgctg ttattactga ttatcgcgac ggcattaagc ctgctcattg
    15841 gggcaaaatc actccccgcg tcagtagtgc tggaggcctt ctcaggcacc tgccagagcg
    15901 ccgactgcac catcgtgctc gacgcgcgac tgccgcgcac ccttgccggt ttacttgcag
    15961 gcggtgcgct tgggcttgct ggggcgttaa tgcaaaccct cacccgaaat ccacttgccg
    16021 accccggctt gcttggcgtg aacgccggag ccagctttgc cattgtgctg ggtgcggcgc
    16081 tgtttggtta ctcatccgcg caggaacaac tggcgatggc cttcgccggg gcgctggtgg
    16141 cctcattgat tgttgccttt atcggcagtc agggcggcgg gcagttaagt ccggtgcgtt
    16201 taaccctggc gggcgtggcg ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc
    16261 tgcttaatcc tgacgtctac gatcaactgc gtttctggca agccggttcg ctggatattc
    16321 gcaatctaca taccttaaaa gtggtgctga tcccggttct gatcgccgga gcaactgcgc
    16381 tattactgag tcgcgcgctg aacagtttga gcctcggcag tgacaccgcg acggcgctgg
    16441 gcagtcgcgt ggcgcgcaca cagttgattg gtctgctggc gattaccgtg ctttgtggta
    16501 gtgcgacggc ggtagttggc ccgattgcct ttatcggcct gatgatgccg cacatggcgc
    16561 gctggctggt aggtgccgat catcgctggt cgctgcccgt cacgctactt gctacccctg
    16621 ccctgctgct gtttgccgat atcatcgggc gggtgattgt gcccggcgaa ctgcgcgttt
    16681 ctgtggtcag tgcgtttatt ggtgcaccgg tgctgatctt cctcgtgcga cgtaaaacgc
    16741 gaggtggtgc atgatttacg tctctcgccg attactcatc acctgtttgc tgctgatttc
    16801 cgcctgtgtg gttgcaggta tctggggatt acgcagcggt gccgtcacgc tggaaatctc
    16861 gcaggtattc gccgcgttga tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga
    16921 atggcgttta ccacgcgtgc tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg
    16981 cgcgattttt cagtcgctga tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa
    17041 caccggggcg tggagcggcg tgctggtggc gatggtgctg tttggtcagg acctgacggc
    17101 tatcgcgctg gcagcaatgg tgggcggcat tgtcacttct ctgttggtct ggctgctcgc
    17161 ctggcgtaac ggtatcgaca cctttcggtt gattattatc ggcatcggcg ttcgcgccat
    17221 gctggtggcc tttaatactt ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc
    17281 gggtttgtgg aatgccggat cgctcaatgg cctgacgtgg gcaaaaactt cgccttccgc
    17341 acccatcatc atattgatgc tcattgccgc cgccttgctg gtgcggcgaa tgcgcttgct
    17401 ggagatgggc gatgataccg cctgcgcgct gggcgtcagc gtcgagcgtt cgcgtctgtt
    17461 aatgatgctg gttgcagtgg tgcttaccgc tgcggcaacg gcgcttgccg ggccgatttc
    17521 ctttattgct ttagtcgcgc cgcacattgc ccgacgcatt agcggcaccg ctcgctgggg
    17581 gctgacccag gcggcgctgt gcggagcgct gttactgctg gcggccgatc tctgcgccca
    17641 acaactgttt atgccgtatc aacttccggt tggcgtcgtt accgtcagcc tcggcggtat
    17701 ttaccttatc gtcttgttaa ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt
    17761 ttgcgcggcg aacagttaac cctgggatat ggcaaatata ccgttgcgga aaatctgact
    17821 gtagaaattc ctgatggtca cttcacggca attatcggac caaatggctg tggtaaatcc
    17881 acgttactgc gtaccttaag ccgcctgatg acgcctgctc acgggcatgt ctggctggat
    17941 ggcgagcaca ttcaacatta cgccagtaaa gaagttgcac gccggattgg tctgttggcg
    18001 caaaatgcta ccacgccggg cgatatcacc gtgcaggagc tggtggcgcg tggacgttat
    18061 ccgcatcaac cgctgtttac ccgctggcgc aaagaggatg aagaagcggt aacgaaagcg
    18121 atgcaggcca cgagaataac tcatctggca gatcaaagcg tggataccct ttctggcgga
    18181 caacgccagc gagcgtggat cgcgatggtg ctagcccagg aaacggcaat tatgctgctc
    18241 gacgaaccca cgacctggct ggatatcagt catcagattg atctactgga actgttaagc
    18301 gaactgaacc gcgagaaagg ctataccctg gcggcggtgc tgcacgatct taatcaggcc
    18361 tgtcgttacg ccagccattt gattgcattg cgggaaggga aaattgttgc tcagggagcg
    18421 ccgaaggaga ttgtcactgc tgaactgatt gagcgcattt atggtctgcg atgcatgatc
    18481 attgacgatc cggtagccgg aacgccgctt gtggtgccgc tcggacgaac ggcaccttca
    18541 accgcaaata gttaaactaa gtggtctgcc atcatggcat cctgttttct ggatgccatc
    18601 gcatagcgca ataacacgct accacaagcc accatcccgc cgatcagcgc ggaaaggatc
    18661 acaataatcg ccttacccgg accgtctttt ttcactggca atgacgggct taactgatat
    18721 ttaaacggcg taaaattcac atcgttgaca tttgcttttg ttaattgctc gacaagatac
    18781 tgccgattac gtaattcacc gttcagttcc gcaacgtcag taaccgcttt ttcaatttcc
    18841 agtttgcgtt caataccgtc tgcgccgaga gaaatagaga aatcggggtc atctttaacg
    18901 gcctgaccat tactgtatac gggcttttta attcctgccg cgttggcaat gtcgagtgaa
    18961 taattgaggc gctgaatgtt tgcatcaagt tgatttttcg ttttaatgcg atcctgagcc
    19021 agtttttctt tttcaaactg ggttttgatc tccagtttat tacggacgtt ttctatcgac
    19081 tctttcacca ccaacgcaga gatataatcg atatacccgc tcaaaacggt ctgcgcctct
    19141 ttactggttg gcgcggtaaa acttagcgtc caggaggtat acagtgacgg ttcatctttt
    19201 ttcttactgg cattgtcatc aaccgctttc attttttcgc tcaatgcaac aatagcgcga
    19261 tgcaaatcca gttcatcgat tttcgcctct tttaattggt ccatcacata aggtgatgaa
    19321 cgcaggtact cttccagcaa gctaacagac tgaaacttct tgataaacag gttaaatgca
    19381 ccgccacggt cgatattaac atcgagatca agaacgcgga gtttggtaaa ggttttctcc
    19441 aggtcctgcc actgaatggc ttcggcggga gtaataacgg ctgagctggt ccatttttgc
    19501 ggcaggatga aagagatcag taaacctgcg caggcaaatg caaaaacgac cgccatgacc
    19561 gtttttttgg cccgccataa aacttcgatt agattaagta aatcaatttc attattactg
    19621 ggcgacgcca gaggatactc agaaaaatga gcgtcacttc cctgtttaat attcaatgat
    19681 gacatggggt ataacctgaa aatgagtcca ataggggaaa atctccacgg cgctcacttt
    19741 accagttggt gagaattatc cgaagctgaa gtttgtaaat tccttccact gaatgcaata
    19801 ttcactctat ttaagagaaa tattcatcaa acctataaat caaacaaata aaaaacccat
    19861 aattattttc tgtaattatg ggtttataga taatattaat ttacctgttt agcgttgcgc
    19921 gaataatcgg cccaattttt acaaacgccc ctggagagat aatatccaca tgcgcacaat
    19981 cctgacgata gatatccagc tccgcaatcc acggcgacca ggcgcgttca ggactcatac
    20041 cttcctgaag cgtgcgttca gcaacaaaca acgtcgcttt accgtcaaac ggtacgctat
    20101 gagcagtcgt cagcaggcgc acagcatcag cgtagttgcc ttcaatggtg gtaaacaact
    20161 ccgttgaagt acttccctgc tgtgccgcca ggaaggcctc gcgctcgcgg ttaatctccg
    20221 ccagcacttc cgggtccaga ccattagctt ctttttcctg ccagttttgc gtttctggcg
    20281 gccaggtatc cagcaagcca agaaatgcca cctgttcgcc acgggcacgc agtcgcgccg
    20341 caataccctg cgccagcgta ccgccaaggg aataccccag caggtaataa gggccgtgcg
    20401 gttgttgctc aagtaacgtt gccagatgcg cttcgcagac ttcatccagg tttgtcgctg
    20461 tctgcatggg tccatgaggg cgcggcgact gaatgccaat aatcgaccat tgtggatcga
    20521 gataacgcga gagtacgcta aactgccagg caaaaccgga cgccggatgg aaacaaaaca
    20581 gcgtcgggcc attaccttca cgcaacggca gaatggtttc gaatcccatg cgccgggagc
    20641 tgtcctcttc accatcaata atcgttgcca gtttggcgac ggttgacgcg accatcacct
    20701 gccccggcgt cacctgacga gcaaactgtc gacttaactg cgctgccagt ttcattgcca
    20761 gtagcgaatg accgcccagc gcaaagaaat cagcatcggc atcctgcacg tcacaaccca
    20821 gcaacgacgc gaatgccgcg gcgataatcg tttcactgcc cgctttcggt gcacgtcccg
    20881 acgcttgtgt cttcagttca ggcaacggta aggctttgcg atccagcttg ccgttggcgc
    20941 taagaggtaa ctgtggaagt tgcagcagaa ccaccggtac catatgtggt ggcaatgttt
    21001 cacgaagctg cgcctgcaat gcgctggtat ccaacggcag gcccgattgc gacaccagat
    21061 agcccaccaa ctgacgcgca tcaccaccag tggctgccgc ctggttaatc acacaggcgt
    21121 gggtaacggc ttgttcgaca tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt
    21181 cgatacgctg cccgcgaatt tttagctgat cgtcactgcg cccgaggtac tccaccgcgc
    21241 cgttatccag ccagcgggca acgtctccgg tacggtacat ccgttcgcca ggagcaaaag
    21301 gatcggcaat aaagcggctg gcggtcagat cggggcgtcc gagatagccc tgcgccagtt
    21361 gaatgccggt aagatagaga tcacccgcca cacccggcgg caccggatgc atcatcgcat
    21421 caagaatacg cagccccatg ttccacaccg gataaccaat cggcacactg ctgccgcgca
    21481 cctgtgccag ttcctcgcca aaagccggat accagctgac atctaccgcc gcttccgtcg
    21541 ggccatacag attatgtaac ggcgcaccgg ttaactgttg ccattcacgg cataaatcag
    21601 ccggtaaggc ttcaccacta cagaaaacct gtttcaacgt cgcgcaactc tggcgagcgg
    21661 tttgcggagt cagcgaggca acaaatgccg ccagcatcga cggcacaaag tgcgtggtcg
    21721 ttacgccata ttcggcaaag aattgctgca tagcgagcgg gtcacggtgc gcttccggtt
    21781 cagccatcac cagttttgcc cctgcgataa acggccagaa aaactcccac accgagacat
    21841 caaaactgca cggcgttttt tgggcaacga catcttcgcc tgtaagcggg tagtgatttt
    21901 gcatccataa caggcggtta acgatagccg tctgcccgac cattacccct ttcggcctgc
    21961 cggtggagcc agaggtaaag atgatataag ccgtgtggtg cggttgtgaa agttgcagcg
    22021 gcgcactgcc ctggggggta agcggggcgt tatagcaaag gcttgttaaa ttgggaacat
    22081 cgctaaagcg cggcagttga tcgtcggtgg taattaacag cgacggacgc gcatcttcca
    22141 gcatcatttt caggcgatcg tccggatagc cggtatccag cggtagccag gccgcgccag
    22201 cttcaactat tgcatggagt gccagggtca aaaagaccga gcgcggtagt gccaccgcca
    22261 cgctgtcccc tggtttaacg ccgcgctcac gcagcagatt cgccagcgcc accacctgct
    22321 cgcgcatttc ccgatagctg aacaggtaac gcgcatctgc cagcgccgga gcatccggtg
    22381 tttttgccgc ttgttctgcc accagcgcgc tcagcgtggt ttctggaatc tcaacctgag
    22441 tggcgttgat ctgcgccagc tgcgcatact cacctggcag cataatatcg acatcgccgc
    22501 acaacagcgc cggatccgcg gcgaactggg caatcagcat tttcaggcgt tcagcatgct
    22561 ggattaacgt tggctcatcg taacgctgtt tattggcgag gatctcaata ctcaaatcac
    22621 cgtgttcatc cgggaacagg gccagttcaa ggtcattaac cggaccggtt gccagggtat
    22681 gggtttgcgc ctgaacacca ggaatatcca gttggtaatc aaatacctta atattgagta
    22741 ccggaccaaa cagcggttca tcacctgccg ctcgcccgct gtcacggaca atttgttcgg
    22801 catcgtaacg ttgatggcga cgcatttttt tcagttgagc cgccagtcgg gttgccagtt
    22861 ccggcagcgt ttcttgcgcc gcaatgtgaa tacccaacgg taaaacgttg agcacgggtc
    22921 cgctagccgt cagcgccgcc gagcccagtc gacgcataaa gataaatccg gcggcatagt
    22981 ccatgcgatt gcacaatcgc cccagccaca aggctgccag cgcaagggct aaatcggtac
    23041 gctgcacacc tgaaagttgc gtagccagct ggcggaattc cccgtcggta aattccagtt
    23101 tcaggcgcag aatatctgcc gaagcgctgc gccccggtaa aggtgccgga gaaagtgacg
    23161 cgggcggcgg cagttgacga cgctgttctg cccagaatgc cgcatcacgc tgccaggctt
    23221 cgctttcgcg gtattgctgg tactcttcca ctacatcagc gaaaggcgta aacggcgaag
    23281 caggcgttgg ttcgccacgc agccatgtgc agtaaatatt ggcgatctgg cgagtaatgg
    23341 ccgggaaact gaagccatcg accagcaaat ggtgataacg ctgataccag taccagcggt
    23401 tatccgcaac atgtatcagc tgatgaaaga ccagtggttt accgctatcg acgcgcaaat
    23461 cttgttgcaa atccgtcagc attaatgcct gcgcagtacc gtgcggatca atattggttc
    23521 gcaggtcgat aatttctggc tgttcgaata tcagcgcatc atcgacccac tgccagactt
    23581 cgccgttatc ttccgtaaaa cgcatccgca acgtatctgc ttgcgctagt ccggcaacca
    23641 ccgcgcgggc cagtaatggc gcatcaacat ctccagttaa ctcaacgtaa tgcgccacgc
    23701 tccaggcgga gggtaaatcc gacagttttt ctgccatcca gatgccgggc tgtgcggcga
    23761 ccaaaggtaa atgctggctc attgtgcctc ctgcaactgg gtgaaattgg ccggtgtcag
    23821 agtacgccag tggacttcca gccactgctg gcaggacgcc tgtgactgcg gctgacacac
    23881 aatgtcccag cctgccgata agaggcattg ttgcggccac agactgaatt gcccctgcgc
    23941 attgcgcaaa atgtaaaacg ctccctgcgg atcatcgaag ggattactga atgccatact
    24001 caactcctgt catggaaaag tggttgccag aggtcgatta acccctgcat caagccaccg
    24061 cgccaacaaa gcgcatcatg tccgccgtca acctgacgcc agaaaatgga ttcttttata
    24121 gggtgcaatt gcgcatacag cgcctgattg gcccgcatga tcatcggctc gcgaataccc
    24181 gcttccagca caatgcgcag accttcggcg ctaacttcac cagcttttag cttttcaagt
    24241 aacacgccct cttgctgccc gtcccgatgc ggccaccagt acgatcctga ctggcttaat
    24301 acacagccaa agcgttcagg ccagtgcagt ccggcataca gcgcggacag cccaccaaaa
    24361 ctctgcccgg cgaccacggt gcgatcggcg cgatcgctaa aaggggcaat agctttcacc
    24421 aggggtaata actcttgctg tactgccagc cagaaatccg cattacacgg cagttcgtgg
    24481 gcgcggtgcg tggtgtcgat agcgtcgatc aacacataca cggcgggagg aagttgctga
    24541 cgatgggtca gcgaagtcag cacaggccag acgggcatac tttgcgccca aaattcgcca
    24601 tcgagcaaaa ctgccagcgg acgttcttct gctgttacat cgccggtggt aaaaatccat
    24661 acacgacgtg aatttttcaa ccgttcactt ttccagataa tttctttggc tgctatttct
    24721 ggcacttgcg gacaatccca tcccggttgc agaggcgctt gcggcatttc gagtgcagaa
    24781 acagcgtgcc ctcgcccgcc tttccagctt tgtggattca gcggatcggc tattgcctgg
    24841 ggtaatagtt ttcgccagcc ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa
    24901 atgtcatcgc gttcggtggg aataaagcag tagctgccgc gccagttggc attgagttgt
    24961 gtcgtccact gccagacatc agtacctgca attcgctgca tcgactgggg ctggctgttc
    25021 tgatgatgat cggtcacacc agtgatgtag atccatacgc gctttatcgt cgagtattct
    25081 tcagaacctt ggggatcacg ccaccagaaa gtgacctcaa acatttcgtc attcagacgc
    25141 tgccattccg ggccatgttt cgactgccac cagctctcac ttcctacttt taacgccgtc
    25201 acaccataac cccatgttta ctgtgcaatt tttcattgat tgcagaaata tattgataat
    25261 attattgata accatttgca tttgcaatag cgtaatggcg cgccgtggga agcgcggaca
    25321 tttattaacc aactgcactg cgtgtctttc aggatcaaag gttttcgcgg tagcgggatg
    25381 cgtcgtgttg aggacgacca tgcccgacag ttgcaattcg tggcaaaaat gcaggaataa
    25441 aacaatgaac aagaagattc attccctggc cttgttggtc aatctgggga tttatggggt
    25501 agcgcaggca caagagccga ccgatactcc tgtttcacat gacgatacca ttgtcgttac
    25561 cgccgccgag cagaacttac aggcgcctgg cgtttcgacc atcaccgcag atgaaatccg
    25621 caaaaacccc gttgcccgcg atgtgtcgga gatcatccgt accatgccag gcgttaacct
    25681 gaccggtaac tccaccagtg gtcagcgtgg gaataaccga cagattgata ttcgcggtat
    25741 gggtccggaa aacacgctga ttttgattga cggcaagccg gtaagcagcc gtaactcggt
    25801 gcgtcagggc tggcgtggcg agcgcgatac ccgtggtgat acctcctggg tgccacctga
    25861 aatgattgaa cgtattgaag ttctgcgtgg tccggcagct gcgcgttatg gcaacggcgc
    25921 ggcgggcggc gtggttaaca tcattaccaa aaaaggcagc ggtgagtggc acggctcctg
    25981 ggacgcttat ttcaatgcac cagaacataa agaggaaggt gccactaaac gcaccaactt
    26041 cagcctgacc ggtccgctgg gcgacgaatt cagcttccgc ttgtatggca acctcgacaa
    26101 aacccaggct gacgcgtggg atatcaacca gggccatcag tccgcgcgtg ccggaacgta
    26161 tgccacgacg ttaccagccg ggcgcgaagg ggtgatcaac aaagatatta atggcgtggt
    26221 gcgctgggat ttcgcgccat tgcaatcgct ggaactggaa gcaggttaca gccgccaggg
    26281 caacctgtat gcgggcgaca cccagaacac caactccgat tcctataccc gctcgaaata
    26341 tggcgatgaa accaaccgtc tgtatcgcca gaactactcg ctgacctgga acggcggctg
    26401 ggataacgga gtgaccacca gcaactgggt gcagtatgaa cacacccgta actcgcgtat
    26461 tccggaaggt ctggcaggcg gtaccgaagg gaaatttaac gaaaaagcga cacaggattt
    26521 cgtcgatatc gatcttgatg acgtgatgct gcacagcgaa gttaacctgc cgattgattt
    26581 cctcgttaac caaacgctga cgctgggtac ggagtggaat cagcaacgga tgaaggactt
    26641 gagttccaat acccaggcgc tgaccggggc gaacaccggc ggcgctattg atggtgtgag
    26701 tgccaccgac cgtagcccgt attcaaaagc agaaattttc tcgctgtttg ccgaaaacaa
    26761 catggagctg actgacagca ccatcgtaac gccggggctg cgtttcgatc atcacagtat
    26821 tgtcggcaat aactggagcc cggcgctgaa catatcgcaa ggtttaggcg atgacttcac
    26881 gctcaaaatg ggcattgccc gtgcttataa agcaccgagc ctgtaccaga ctaacccgaa
    26941 ctacattctc tacagtaaag gtcagggctg ctatgccagc gcgggcggct gctatcagca
    27001 aggtaacgat gacctgaaag cagaaaccag catcaacaaa gagattggtc tggagttcaa
    27061 acgcgacggc tggctggcgg gcgtcacctg gttccgtaac gattatcgca ataagattga
    27121 agcgggctat gtggctgtag ggcaaaacgc agtcggcacc gatctctatc agtgggataa
    27181 cgtgccgaaa gcagtggttg aaggtctgga aggatcgtta aacgtaccag ttagcgaaat
    27241 ggtgatgtgg accaataaca tcacttatat gctgaagagt gaaaacaaaa ccacgggcga
    27301 ccgtttgtcg atcatcccgg agtacacgtt gaactcaacg ctgagctggc aggcgcggga
    27361 agatttgtcg atgcaaacga ccttcacctg gtacggtaag cagcagccga aaaaatacaa
    27421 ctacaaaggt cagccagcgg ttggcccgga aaccaaagaa atcagtcctt acagcattgt
    27481 gggtctgagc gcgacctggg atgtgacgaa gaatgtcagt ctgaccggcg gcgtggacaa
    27541 cctgttcgac aaacgtttgt ggcgtgcggg taatgcccag accacgggcg atctggcagg
    27601 ggccaactat atcgcgggtg ccggggcgta tacctataac gagccgggac gtacgtggta
    27661 catgagcgta aacactcact tctgatgcta acgtcagatt gttgacaaag tgcgcgtcgt
    27721 tcatgccgga tgcggcgtga acgccttatc cggcctacaa aaatcttgcc aattcaatat
    27781 attgcaggac catgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgtcatc
    27841 agtctcgaat atggtcgata tgaaaactac gcatacctcc ctcccctttg ccggacatac
    27901 gctgcatttt gttgagttcg atccggcgaa tttttgtgag caggatttac tctggctgcc
    27961 gcactacgca caattgcaac acgctggacg taaacgtaaa acagagcatt tagccggacg
    28021 gatcgctgct gtttatgcct tacgggaata tggctataaa tgtgtgcccg caatcggcga
    28081 gctacgccaa cctgtctggc ctgcggaggt atacggcagt attagccact gtgggactac
    28141 ggcattagcc gtggtatctc gtcaaccgat tggcattgat atagaagaaa ttttttctgt
    28201 acaaaccgca agagaattga cagacaatat tattacacca gcagaacacg agcgactcgc
    28261 ggactgtggt ttagcctttt ctctggcgct gacactggca ttttccgcca aagagagcgc
    28321 atttaaggca tcggagagac aaactgaagc aggttttctg gattatcaga taattagctg
    28381 gaataaacag caggtcatca ttcatcgtga gaatgagatg tttgctgtgc actggcagat
    28441 aaaagaaaaa atagtcataa ctctgtgcca acacgattaa ttgacaacat ctggtacgat
    28501 tcgcccgcag ccatcactga ctgcgggcga aagtgtaaag caggtgcctt accactctga
    28561 cctgacaacc ggatatgcag ggattgctcc ccgcacaacg gctacttctt cggttcgtaa
    28621 gcgagaacag ccttaaactc aatattacgt tcctttacgg tgaactcaca cagcgagtct
    28681 ccgaccagaa gcgtaaatcc cagcaccgtc aaacacagca ctatgactgc cacaagggca
    28741 tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat ccgagttgtt
    28801 gaggttcgga gtggcagccc caggattgat agaaatatct cctggggctt catctttcca
    28861 gacctcagag taaacctgaa accggaaagc ttcaggcacc cgccgttatc ttacccttcc
    28921 tcagcaaaat atctgtttat ttatacagta agtcctgttt cggacttctg ttggcaacac
    28981 tcgttattgc tcgtaaaacg aaatcgcccg cagccatcac tgaccgcggg cgaaagtgta
    29041 aagcaggtgc cctaccatcc tgacctgaca accggatatg cggggattac tccccgcaca
    29101 acggctactt cttcggttcg taagcgagaa cagccttaaa ctcaatatta cgttccttca
    29161 ccgtaaactc acacagcgag tctccgacca gaagcgtaaa tcccagcacc gtcaaacaca
    29221 gcactatgac tgccgcaagg gcatatttcg tcagcatgtt tatattgcct ccatgcgaag
    29281 aagggactac catccaaatt gttcgggttt ggagtggcag ccccagggtt gatagaaata
    29341 tctcctgggg cttcatcttt ccagacctca gagtaaacct gaaaccggaa agcttcaggc
    29401 acccgccgtt atcttaccct tcctcaacaa aatggctgtt tatttataca gtaaatcctg
    29461 tttcggactt ctcactcctc atttaatcaa ctgaagctga gcccgttttt tctgcatgcc
    29521 tcaatcgtca aaattcttcg ccatcagcca gctctgccat aacttgccac attgaaaaag
    29581 tagccaaatt gcttcagtca ccatcatcaa aaaaagaaac attccagaac ttccgcatct
    29641 cgcccagccg ctccgacttc tatactgaat agaaaacgcc aacataagag aaacctatgc
    29701 cattacccga ttttcatgtt tctgaacctt ttaccctcgg tattgaactg gaaatgcagg
    29761 tggttaatcc gccgggctat gacttaagcc aggactcttc aatgctgatt gacgcggtta
    29821 aaaataagat cacggccgga gaggtaaagc acgatatcac cgaaagtatg ctggagctgg
    29881 cgacggatgt ttgccgtgat atcaaccagg ctgccgggca attttcagcg atgcagaaag
    29941 tcgtattgca ggcagccgca gaccatcatc tggaaatttg cggcggtggc acacacccgt
    30001 ttcagaaatg gcagcgtcag gaggtatgcg ataacgagcg ctatcaacga acgctggaaa
    30061 actttggcta tctcatccag caggcgaccg tttttggtca gcatgtccat gttggctgcg
    30121 ccagtggcga tgacgccatt tatttgctgc acggcttgtc acggtttgtg ccgcacttta
    30181 tcgccctttc cgccgcgtcg ccatatatgc aaggaacgga tacgcgtttt gcctcctcac
    30241 gaccgaatat tttttccgcc tttcccgata atggcccgat gccgtgggtc agtaactggc
    30301 aacaatttga agccctgttt cgctgtttga gttacaccac gatgatcgac agcattaaag
    30361 atctgcactg ggatattcgc cccagtcctc attttggcac ggtggaggtt cgggtgatgg
    30421 ataccccgtt aacccttagc cacgcggtaa atatggcggg attaattcag gctaccgccc
    30481 actggttact gacagaacgc ccgttcaaac atcaggagaa agattacctg ctgtataaat
    30541 tcaaccgttt ccaggcctgt cgctatgggc tggaaggcgt cattaccgat ccgcacactg
    30601 gcgatcgtcg accgctaacg gaagacacct tgcgattgct ggaaaaaatc gccccttccg
    30661 cacataaaat tggtgcatcg agcgcgattg aggccctgca tcgccaggtc gtcagcggtc
    30721 tgaatgaagc gcagctgatg cgcgatttcg tcgccgatgg tggctcgctg attgggctgg
    30781 tgaaaaagca ttgtgagatc tgggccgatg actaagtagg gaattgcctt acagaagaat
    30841 atccctgaca atggtgtttt aacttacatt taacaatgtt atgaaacacc ctttagaaac
    30901 cttgactacc gcagcaggca ttttgctgat ggctttcctc tcttgcctgc tgctgcccgc
    30961 ccccgcactg gggctgacgc tggcacaaaa actggtgacc atgttccatc tgatggatct
    31021 tagctagctt tacactttat tgttttgtct gtggttttta gtgctgggcg ctattgagta
    31081 ttttgttctg cgctttatct ggcgacgctg gttctcgctg gcggattaaa cgtcgattat
    31141 cgccgtactt cgcggcatac tttgcttatt ctccttcgcc taaaggaacg tttatggata
    31201 agcaatcact gcacgaaacg gcgaaacgcc tggcccttga gttacccttt gtcgagcttt
    31261 gctggccttt tggcccggag ttcgatgttt ttaaaattgg cggcaaaatt tttatgctgt
    31321 cgtcggagct gcgcggcgtc ccctttatca atctgaagtc cgatccacaa aaatccctgt
    31381 taaatcagca gatatacccg agcattaagc cagggtatca catgaataaa aagcactgga
    31441 tttcggtgta tcccggcgag gaaatctccg aagcgttact tcgcgatctg atcaacgatt
    31501 cgtggaatct ggtggttgat ggtctggcta aacgcgatca aaaaagagtg cgtccaggct
    31561 aaagcggaaa tctatagcgc atttttctcg cttaccattt ctcgttgaac cttgtaatct
    31621 gctggcacgc aaaattactt tcacatggag tctttatgga tatcatttct gtcgccttaa
    31681 agcgtcattc cactaaggca tttgatgcca gtaaaaaact taccccggaa caggccgagc
    31741 agatcaaaac tctcctgcaa tacagcccat ccagcaccaa ctcccagccg tggcatttta
    31801 ttgttgccag cacggaagaa ggtaaagcgc gtgttgccaa atccgctgcc ggtaattatg
    31861 tgttcaacga acgtaaaata cttgatgcct cgcacgtcgt ggtgttctgt gcaaaaaccg
    31921 cgatggacga tgcctggctg aagctggttg ttgaccagga agatgctgat ggccgctttg
    31981 ccacgccgga agcgaaagcc gcgaacgata aaggtcgcaa gttcttcgct gatatgcacc
    32041 gtaaagatct gcatgatgat gcagagtgga tggcaaaaca ggtttatctc aacgtcggta
    32101 acttcctgct cggcgtggcg gctctgggtc tggacgcggt acccatcgaa ggttttgacg
    32161 ccgccatcct cgatgcagaa tttggtctga aagagaaagg ctacaccagt ctggtggttg
    32221 ttccggtagg tcatcacagc gttgaagatt ttaacgctac gctgccgaaa tctcgtctgc
    32281 cgcaaaacat caccttaacc gaagtgtaat tctctcttgc cgggcatctg cccggctatt
    32341 tcccctcaga ttctcctgat ttgcataacc ctgtttcagc cgtcatcata ggctgctgtt
    32401 gtataaagga gacgttatgc aggatttaat atcccaggtt gaagatttag cgggtattga
    32461 gatcgatcac accacctcga tggtgatgat tttcggtatt atttttctga ccgccgtcgt
    32521 ggtgcatatt attttgcatt gggtggtact gcggaccttc gaaaaacgtg ccatcgccag
    32581 ttcacggctt tggttgcaaa tcataaccca gaataaactc ttccaccgtt tagcttttac
    32641 cctgcagggg attatcgtca atattcaggc ggtattctgg ctgcaaaaag gcaccgaagc
    32701 ggcagatatt ctgactacct gcgcgcagtt gtggatcatg atgtatgcgc tgctttcagt
    32761 cttctcgttg ctggatgtta ttttgaatct ggcgcagaaa ttcccggcgg catctcagtt
    32821 accgctgaaa gggatatttc aggggattaa actgatcggc gcgatcctgg tcggcatttt
    32881 gatgatctcg ctgctgattg gtcagtcgcc agcgattctg atcagcggtc ttggtgcaat
    32941 ggctgccgtg ctgatgttgg tatttaaaga tccgattctt ggtctggtgg ccggtattca
    33001 gctttccgcg aacgatatgc tgaaactggg cgactggctg gagatgccga aatacggcgc
    33061 agatggcgcg gtgatcgata ttgggttaac caccgtcaaa gtgcgtaact gggacaatac
    33121 cattaccact attcccacct ggtctctggt ttctgactcc tttaaaaact ggagcgggat
    33181 gtcagcatct ggcgggcgac gtattaagcg cagtatcagt attgatgtca ccagtattcg
    33241 ttttcttgat gaagacgaaa tgcaacgtct gaataaagcg catttgttaa agccttattt
    33301 aaccagccgc catcaggaaa ttaatgagtg gaatcgccag caaggttcta cggagtcggt
    33361 attaaacctg cgccgaatga ccaatattgg aacctttcgt gcctatctga acgaatatct
    33421 gcgtaaccat ccgcggattc gtaaagatat gaccttaatg gtacgccaac tggctccagg
    33481 tgataacggt ttaccgctcg agatctacgc gtttactaac accgtggtgt ggctggaata
    33541 tgaaagcatt caggctgata tattcgatca catatttgcg attgtcgaag agtttggtct
    33601 gcgacttcat cagtcgccaa ccggcaatga tattcgctct ctggcgggtg catttaagca
    33661 gtaattaaaa aaaccgctct catcgaatgg atgagagcgg acatcgtttt gtcggatgta
    33721 gcgtgaatgc cttatttccg acgcagcgtt ttaaatgcca caaacaggaa tacaatccac
    33781 accggcagca ggatcgctga caagcgcata tcatccatcg tgcacatcag cagcaaaatc
    33841 atgccgagga aggcaatgca gagatagttt ccgaacggat agagcagcgc cttaaactgt
    33901 gtttcacgcc cctgacgtcg catcgctgca cgaaaacgca gatgcgccag acagatcata
    33961 atccagttca acagcagcgt tgctaccacc agcgccatca gcagaccaaa cgctttttgc
    34021 ggcagcagat agttgattaa caccaccagc gaagtgatcg ctccggaaag catcagcgag
    34081 ttaatcggca caccgcgacg gctgacgcga gtcaaaaact tcggcgcatt accctgcaca
    34141 gaaaggccaa acagcatgcg gctgttagag taaaccccgc tgttatacac tgacagcgat
    34201 gctaccagaa tgacgaagtt cagcgcagaa gctaccacgt tgctgtcgag attatggaaa
    34261 atcatcacaa acgggctact gttggatttc acttccaccc acggatagag cgccagtaaa
    34321 accaccagtg aaccgatgta aaacagcagg atgcgataca ccacctgatt taccgctttt
    34381 ggaatgcttt tatccggatc gcgcgcttca gcggcagtaa tcccaatcag ctccagaccg
    34441 ccgaaggaga acataattac cgccagcgac aaaatcagcc cattccagcc ggtggcgaag
    34501 aaaccaccgt agcgccagag gttgtcgata ctggctttct cgccgccgtg accagaaaac
    34561 agcagccaca ggccaaagcc gatcataccg atgattgcca gcactttaat caacgcaaac
    34621 cagaactcgg tttcgccata taagcgcacg ttcaccaggt taacggcgtt gatgataata
    34681 aagaaggcgg cagcccaaat ccacgttgga acatccggga accagtactg catatagatg
    34741 cccgcagcgg tcagctctgc cattcccacc agcacgaaca ttacccagta gttccagcca
    34801 gagaggaagc ccgcaaacgg tccccagtat ttataggcaa agtgggcaaa tgaaccggat
    34861 accggctctt cgaccaccat ctcgccaagc tggcgcataa tcaggaaagc gatgatcccg
    34921 gcgacgccgt agcccagcaa tacagccgga cccgccatct gaatcgccgg accaatgcca
    34981 agaaacagac cagtaccaat tgcgccaccc agcgcaatca gttgaatatg acggttatgt
    35041 aatccgcgat gaagcgtcgg ctcttgattc gacgcagtat cttccgatac ggttgacgcg
    35101 tttttcacgc ctttcccctg tgtgtctttt ttgttgaggg gcacctttta acatttagtg
    35161 cccatcgtcg caagacacaa tccacacggt taaacggggt atcctgcttt tatttccgta
    35221 cccgatgtcg gtgcagccac atcagcttat acgccgccgg gataataaac agcgacagca
    35281 aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat ccggctcatc acctctgaac
    35341 cagcccccgt tccccacaga atcggcagca gaccggcgat aatcaccgcc accgtcatcg
    35401 ctttcgggcg cacgcgcagt accgcgccgt gatacaacgc ctcatccagc ttctgctcac
    35461 tgaatgtttg cggattattc aacgacggct cggcctctat ggcgtgacgt aaatacatca
    35521 gcatcaccac gccaaattcg gcggcgaccc cggcaagggc aataaaaccg gtgcccgttg
    35581 ccacagaaag atgaaagccc atccaccaca gcaaccagat gccgcccacc agcgcaaacg
    35641 gtacgctgct gataatcagc aacgcttcgc ccacccgacg gaatgccaga tacaacagca
    35701 cgaagatgat catcagtgtc atcggcacca tgagtttcag cttatggttg gcgcgctcca
    35761 gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg ttttaactgc actttttcag
    35821 ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc acgatcgcgg gcatcgatat
    35881 aaatccagct cgtcgggcgc gcattctcgg ttttcagcat cgacggtccg gtagagactt
    35941 taacgtcggc cacgtctgcc agggtgattt gctgcttcat cggcgtcagg atcggtagct
    36001 ggcgcagtgc ctgcgggcta tcgcgccagc tttgcggata acgcagatta atgggataac
    36061 gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc caccgcagaa gtcacaaaca
    36121 actgcacatc cgccaccgtc ataccgtaac gcgcggcttt ttcacggtta atctcaacgt
    36181 tgatatagcg cccaccttcc agacgctcgg ctagagcaga agccacgctt ggcaccgttc
    36241 gcgctacttc ttcaatttgc tcagccatcg cgtcgatatc cgccagcaca gtgccggaaa
    36301 ctttaatgcc gatggggctt ttaatgccgg ttgagagcat atcgatacgg ttacgaattg
    36361 gcggcaccca cagattcgcc agccccggca gccgcacggt gttatccagt tcctcaatga
    36421 ttttgtccat cgtcatgcca ggccgccact gatcctgcgg cttaagctgg atggtcgttt
    36481 caaccatctc gagcggagca gaatcggtgg cggtttccgc tttcccggtt ttgccaaata
    36541 cccgcgccac ttcaggtacg ctcataatta gcttgtcggt tttttgcagc atactcgccg
    36601 cctctgctgc ggaaatcccc ggcagcgtcg atggcatata caacaagtcg ccttcattga
    36661 tctgcggtaa aaattccccg ccaactttat tgagcggcca gagaaccgtc agcaccgaaa
    36721 gcgctgccac caacagcgtg gttttcggcc agtgcagtac tttcagcaat agaggatgat
    36781 aaacacgaat caaaaagcga ttgagcgggt tactgctttc cggcggaatt ttgccacgga
    36841 tccagtaccc catcaggata gggatcacta cgatcgccag cagcgccgcg cccgccatcg
    36901 cataggtttt agtgaacgcc aacgggccaa acagacgccc ttcctgccct tccagggtga
    36961 agatcgggat aaacgacaac gtgataatca gcagactgat aaatagcgcc ggacccactt
    37021 caacagatgc atcggtgatc acctgccagc gcgttttatt atccagcgtg gcgtcaggat
    37081 gctggtgctg ccactcttcc agccgtttat gtgcattttc gatcatgacg atagcggcat
    37141 cgaccatcgc cccgacggca atcgcaatgc cgcccaacga cataatattg gcattcagtc
    37201 cctggaagtg catgacaata aaagcaatac acaaccccag cggcaacgaa ataatcgcca
    37261 ccagcgccga gcgcacatgc cagagaaaca gcgcacagac taccgccacc acgataaact
    37321 cttccagcaa cttgccgctg aggttgtcga tggcgcggtc aatgagctgg ctgcgatcgt
    37381 atgttgtaac tatctccacg ccttccggca gactactttt cagcgtttcc agtttgtcct
    37441 tcacggaggc gatcacttct cgggcgtttt tgccggagcg caggatcacc accccgcccg
    37501 ccacttcgcc ttcgccattc agttcggcaa tgccccggcg catctccggg ccaatctgga
    37561 ccttcgcaac atcacgcaga taaacgggca cgccattttc actggctttt aaaacgatgt
    37621 gattaaagtc gtcgagcgtt tgcagatagc cgctggcgcg caccatatat tccgcttccg
    37681 ccagttcgat cgacgaaccg cccgcttcct ggtttgaagc atccagcgcg ctttttactt
    37741 cggctagact gatgccatat tgcgccaggc gctggggatc gataaccacc tgatactctt
    37801 tcaccacgcc gcccaccgac gccacttccg caacgtcagg gatggttttt agctcatatt
    37861 tgagaaacca gtcctgcaat gagcgtaaat cggccagatc gtgcttaccg ctgcgatcca
    37921 ccagtgcata ttcatagatc cagccaacac ccgtggcatc tggtcccagc tcggcgctga
    37981 ctcccgcagg cagcttaccc tgtacctggt tgaggtactc cagcacccgc gaacgcgccc
    38041 agtacggatc ggtaccatct tcgaaaatga catacacata agagtctcca aactgcgaga
    38101 aaccgcgcac agtcttcgcg ccaggcaccg acaacatggt ggtggttagc ggataagtca
    38161 cctgattttc aacgatttgc ggtgcctgac cgggatagct ggttttaata atcacctgca
    38221 catcggagag atccggcagc gcatccactg gcgtgttaat gatggtccag gtgccccaga
    38281 tgctcagaaa caacgcgccc atcagcacca aaaaacggtt cgccaccgag cgacgaataa
    38341 tccattcaat cattggttat tccctcaatg cgcattggta gcactttcag agcgcatccg
    38401 ctccagtgcg ccagaaatat tggcttcgga atcaatcagg aacaggccgc tggaaaccac
    38461 cttttcacct tccgccagac cagagtgtaa cgcggtgacg ccttgcgatg cctggaaaac
    38521 ggcaacgcgt ttcggtacaa agcgtccgtc ggcatcaacg gtaatcaccc gctgttcgtt
    38581 gccggtatca atcagcgcct gtgacggaat gagcagcatc ggttcgctgg cggtgttgag
    38641 ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg gcgttgtcga cttccagacg
    38701 cagctgcagc gtgcgggtcg cggcatccac gccaggtagc agcgtccatt tgcggatggt
    38761 gagtgtttta tccggtcgcg ccggaacggt gagcgtaaac tgcgaggcat ctttcaccag
    38821 ccaggcgata gactccggga tcgcagcagt gacccacacc gggtccatac cctgaatttt
    38881 cgctaccacg ttatctttgg cgatattcat tcccgcgcgc agatcaaacg cggtgatcac
    38941 gccatcaatg ggtgctttga gcgtaaagcg agtctggatt ttttgcgtgg cgatcaggcg
    39001 gcgaatatcc gcctccggca ttcccgccag tcgcagccgc tcaagaatgc cttcagtctg
    39061 ggtcgccgta ccgccggttt cgcgcagcag taaatactca ctctgcgctt ccacccagtc
    39121 aggaatggtc aggtcgagaa gcggtgtgcc cttttgtact ttatcgccca cggtaagcgg
    39181 atacaccttg tcgataaacc cggcagcgcg agcctgcaca atggcatact gatactcgtt
    39241 gtaactgaca ttcgccggga aactctgggc aaaagtcagc ggtccgcgcg tgacggtagc
    39301 cgttttcacc cccagattct gcgtctgagt cgggtcaatg cgcacaccag acgcagaact
    39361 ctcttcatcg gcatatttcg gcaccagatc catatccata aacggcgatt tacctggttt
    39421 atcgaaccgc gtattggggt acatcgggtc gtaccagaat aagattttac gttctgcggt
    39481 cgacgttttt tctgcgggtg gttcagcctt tgcaaaccag gtaaaacctg ccgcagaaat
    39541 aataccgccc gcgatcatgc tgccgataat aagcgcgatt tttttcatct cattaaacct
    39601 gggttactgg ctgactttaa tatcctgtaa taaagaaagg ttgccctgct ggacaaaatt
    39661 aaacgccact ttgtcgccgg ttttaatttc actcattttc gtctgcgggg tgatggtaaa
    39721 gcgcatagtc atctccggcc agttcacggc agcaatcgga tcgtgatgga tggtgatttt
    39781 tttgctttcc agatcaatac cctttaccac gccagtggcg ctaataacct gcggttgtac
    39841 ttcgctcatg gtttcatgat gatgttcgtt agcctgggca ttaaagccaa taacggtaaa
    39901 cagactgaac attgcgactt gcagtgcttt tttcatttat tctctcctgg agttaaaaat
    39961 tattgctgcc aaccgccccc taaggcggta tacaaagaaa tttcgttaac ctgacgggca
    40021 taattcaggt cgagtaaagt ttgtcgggtt gcaaataaag aacgctcggc atccagcact
    40081 tccagataac ttaccgcgcc gtgctgatat aatgcccgcg cccgttgcaa agtaatttgc
    40141 agcgacgcca gataacgctg ctgggcgctg atttgatcgt tcaggctttg acgtaatgcc
    40201 agcgcatctg ccacttcttt aaaggcgttc tggattttct gttcataatt cactaccgac
    40261 tgctgctggc gaatttcggc gatatccaga ttggcctggt tgcgtccggc attaaaaatg
    40321 gggatctcaa ttttaggaat aaaattccac atcccgctgc tagcgttaaa taatgaagac
    40381 agatcgctgc tggcggttga tattccgctg gtcaggctga tagacgggaa aaatgccgca
    40441 cgcgcagcac caatattagc attagccgcc attaacgcgt gttcagcttc cataatatcc
    40501 gggcgctgca ataagatttg cgacgacaag cccgccggta atttaacgct ttgcaggctg
    40561 ccgctgttta ctgtctgcgc ttgcggcagt ttgccgtagc ttcccaataa cagttgcaat
    40621 gcattattcg cctgcgccag ttccccctga cgtttagcga tgtcactgcg ggtactttct
    40681 atcaccccgc gagcctgttc cagcgccaga acattgctgc taccggtcaa cagttgtttt
    40741 tcgacaaacg catatgactg ctgataatta cgcagcgttt cttcggctat ttgcaattgc
    40801 gcatacgcca gttgctgatt gaaatagctt tgcgcgacat tagaaaccag cagaatatgc
    40861 accgcgcgct gagcttcctc agtggctaaa taattttgtc gctcggcttc gctcatgttc
    40921 tttaagcgac cgaaaaaatc gaggtcaaag ctggcgttaa ggccggtcga aaactcccgt
    40981 gtcgtggcgg tattcccttt aagattaccg ctccagctgc cgctgccctc gccattgagc
    41041 tgtgggtagc ggtcggcatc ggtcagacga tattgcgccc gtgcctcctg cactttcagc
    41101 gtcgccatgc gcaaatcccg gttattcacc agcgcctcgc taatcagcgt cttcacctga
    41161 ttatcaacaa aaaaggtgcg ccagcccacg ttctggtagt tatctgctgc gttaaccaag
    41221 ccgttctggc tgagtgagaa ctgctgcggc acgggcattg ccgtacgctg ataatccggt
    41281 gccaatgaac aaccggttag cgcaagggcc acacaaaatg gcagaagttt acaaggagac
    41341 ataggctcat aatttctggt gattttatac cgccaacttt actcgccagg ctctgatttt
    41401 ccggtgacag gaaaatgaca aaattgtcat tttgccaata agcgattgcc atctgatccc
    41461 gctactctag aattgcccgg gcaacatgcg gaggaaaaat gaaactgttg attgtcgaag
    41521 atgaaaagaa aaccggagaa tacttgacca aagggttaac cgaagccggt tttgtggtcg
    41581 atttggccga caacgggctg aatggctacc atctggcgat gaccggtgat tatgacctga
    41641 taatcctcga tattatgctg ccggacgtga acggctggga tatcgtgcgc atgttgcgct
    41701 ccgccaataa agggatgccg attctgttgc ttaccgcgct tggcaccatt gaacatcgcg
    41761 tcaaggggct ggagttgggg gccgatgact atctggtgaa gccgttcgct tttgctgaac
    41821 tgctggcgcg ggtgcgcact ctcctgcggc gcggggcggc ggtgattatc gaaagtcagt
    41881 ttcaggttgc cgatctgatg gtcgatctcg tcagccgcaa agtcacccgc agcggcacgc
    41941 gcatcactct gaccagtaaa gagtttactc tgctggagtt ttttctccgc catcagggcg
    42001 aagtgctgcc ccgctcgctt atcgcctcgc aggtgtggga catgaatttc gacagcgaca
    42061 ctaacgccat tgatgtggcg gtgaagcggc tacgcggcaa aatcgacaac gactttgagc
    42121 cgaagctgat tcagaccgtg cgcggcgtgg gctatatgct tgaggtgccg gatggtcagt
    42181 aagccatttc agcgcccgtt ttcgctggca acccgtctga ccttttttat cagcctggcc
    42241 accatcgcgg cgtttttcgc ctttgcatgg atcatgatcc actcagtgaa agtgcatttt
    42301 gccgagcagg atattaatga tttaaaagaa attagcgcca cccttgaacg ggtacttaat
    42361 catcctgacg agacgcaagc ccgacgctta atgacgctgg aagatatcgt cagtggttat
    42421 tccaacgtgt tgatttccct ggcagatagc cacggcaaaa cggtgtatca ctcccccggt
    42481 gcgccggata tccgcgagtt tacgcgtgac gccatacccg ataaagacgc tcagggtggc
    42541 gaggtgtatc tcctttccgg cccgacgatt atgatgccag gccacggtca cgggcatatg
    42601 gaacacagca actggcggat gattaacttg ccggttggcc cgttggtgga cggcaaaccg
    42661 atttatacgc tctacatcgc gctttcgatc gattttcatc ttcattacat aaatgatttg
    42721 atgaataaac ttattatgac cgcatctgtc atcagcatcc tgatcgtctt tatcgtgcta
    42781 ttggcggtac ataaaggtca cgcgccgatc cgcagcgtca gccgtcaaat ccagaatatc
    42841 acctcgaaag atctcgacgt tcgccttgac ccgcagaccg agcccattga gctggaacag
    42901 ctggtactgt cgttcaacca tatgatcgag cgtattgagg atgtctttac ccgccagtcc
    42961 aatttctcag cggatatcgc tcacgaaatt cgcacgccga ttaccaatct catcacgcaa
    43021 acggaaatcg ccctcagcca gtctcgcagc cagaaggagc tggaagatgt gctctactct
    43081 aatctcgaag agctgacgcg aatggcgaaa atggtcagcg atatgctgtt tctcgctcag
    43141 gccgataaca accagctaat ccccgaaaag aaaatgctca acctggcgga tgaagtcggc
    43201 aaagtgttcg attttttcga ggcgttagcg gaagatcgcg gcgtggagtt gcgatttgtt
    43261 ggcgacaaat gtcaggttgc gggcgatccg ctgatgctgc gtcgggcgtt aagcaacctg
    43321 ctttctaatg ccctgcgtta tacgccaacc agagagacaa ttgtagtgcg ctgtcagacg
    43381 gtcgatcacc aggtgcaagt taccgtcgaa aaccccggta cgcccattgc gcccgagcac
    43441 ttaccgcgat tgtttgaccg tttctaccgc gttgaccctt cccgccagcg aaaaggcgaa
    43501 ggtagcggca ttgggctggc gatagtgaaa tcgattgttg tcgcgcataa aggcacggtt
    43561 gcagtaacgt cagatgcgcg ggggacaagg tttgtgatta tgctgccgga gagagagtga
    43621 ttattaagtt caaattcagg gcgtggtgca tcacctcctg gtattgttga tcaacaa
//
LOCUS       DAZPEP010000017        43607 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZPEP010000017 DAZPEP010000000
VERSION     DAZPEP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43607
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.017"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="ACHUT9_000936"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="ACHUT9_000936"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFR1347482.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2667)
                     /locus_tag="ACHUT9_000937"
     CDS             complement(1672..2667)
                     /locus_tag="ACHUT9_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1347483.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="ACHUT9_000938"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="ACHUT9_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUT9_000939"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="ACHUT9_000939"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFR1347484.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUT9_000940"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="ACHUT9_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFR1347485.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUT9_000941"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="ACHUT9_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFR1347486.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUT9_000942"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="ACHUT9_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFR1347487.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUT9_000943"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="ACHUT9_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFR1347488.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKNN"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUT9_000944"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="ACHUT9_000944"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFR1347489.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUT9_000945"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="ACHUT9_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFR1347490.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUT9_000946"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="ACHUT9_000946"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFR1347491.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUT9_000947"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="ACHUT9_000947"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFR1347492.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="ACHUT9_000948"
     CDS             complement(17699..18322)
                     /locus_tag="ACHUT9_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFR1347493.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHUT9_000949"
     CDS             18619..18894
                     /gene="ypdI"
                     /locus_tag="ACHUT9_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFR1347494.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="ACHUT9_000950"
     CDS             complement(18971..19213)
                     /locus_tag="ACHUT9_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFR1347495.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHUT9_000951"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="ACHUT9_000951"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFR1347496.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHUT9_000952"
     CDS             20842..20913
                     /gene="ypdK"
                     /locus_tag="ACHUT9_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFR1347497.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHUT9_000953"
     CDS             complement(20978..22216)
                     /gene="alaC"
                     /locus_tag="ACHUT9_000953"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFR1347498.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            22593..24290
                     /gene="ypdA"
                     /locus_tag="ACHUT9_000954"
     CDS             22593..24290
                     /gene="ypdA"
                     /locus_tag="ACHUT9_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFR1347499.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            24305..25039
                     /gene="ypdB"
                     /locus_tag="ACHUT9_000955"
     CDS             24305..25039
                     /gene="ypdB"
                     /locus_tag="ACHUT9_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFR1347500.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            25052..25909
                     /locus_tag="ACHUT9_000956"
     CDS             25052..25909
                     /locus_tag="ACHUT9_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1347501.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="ACHUT9_000957"
                     /pseudo
     CDS             complement(25912..28407)
                     /gene="ptsP"
                     /locus_tag="ACHUT9_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="ACHUT9_000958"
     CDS             complement(28432..29469)
                     /gene="ypdE"
                     /locus_tag="ACHUT9_000958"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFR1347502.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="ACHUT9_000959"
                     /pseudo
     CDS             complement(29469..30554)
                     /gene="ypdF"
                     /locus_tag="ACHUT9_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30569..31816)
                     /locus_tag="ACHUT9_000960"
     CDS             complement(30569..31816)
                     /locus_tag="ACHUT9_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFR1347503.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMLGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31838..32164)
                     /locus_tag="ACHUT9_000961"
     CDS             complement(31838..32164)
                     /locus_tag="ACHUT9_000961"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFR1347504.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32384..33349)
                     /gene="glk"
                     /locus_tag="ACHUT9_000962"
     CDS             complement(32384..33349)
                     /gene="glk"
                     /locus_tag="ACHUT9_000962"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFR1347505.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            33553..34809
                     /locus_tag="ACHUT9_000963"
     CDS             33553..34809
                     /locus_tag="ACHUT9_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFR1347506.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34924..35250
                     /gene="ypeC"
                     /locus_tag="ACHUT9_000964"
     CDS             34924..35250
                     /gene="ypeC"
                     /locus_tag="ACHUT9_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFR1347507.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35391..36629)
                     /locus_tag="ACHUT9_000965"
     CDS             complement(35391..36629)
                     /locus_tag="ACHUT9_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFR1347508.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36965..38167
                     /gene="nupC"
                     /locus_tag="ACHUT9_000966"
     CDS             36965..38167
                     /gene="nupC"
                     /locus_tag="ACHUT9_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFR1347509.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38217..40405)
                     /locus_tag="ACHUT9_000967"
                     /pseudo
     CDS             complement(38217..40405)
                     /locus_tag="ACHUT9_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            complement(40614..40689)
                     /locus_tag="ACHUT9_000968"
     tRNA            complement(40614..40689)
                     /locus_tag="ACHUT9_000968"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40654..40656),aa:Ala,seq:ggc)
     gene            complement(40729..40804)
                     /locus_tag="ACHUT9_000969"
     tRNA            complement(40729..40804)
                     /locus_tag="ACHUT9_000969"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40769..40771),aa:Ala,seq:ggc)
     gene            41025..41384
                     /locus_tag="ACHUT9_000970"
     CDS             41025..41384
                     /locus_tag="ACHUT9_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1347510.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41386..41778
                     /locus_tag="ACHUT9_000971"
     CDS             41386..41778
                     /locus_tag="ACHUT9_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFR1347511.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="ACHUT9_000972"
     CDS             complement(41830..43245)
                     /gene="gltX"
                     /locus_tag="ACHUT9_000972"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFR1347512.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43504..43579
                     /locus_tag="ACHUT9_000973"
     tRNA            43504..43579
                     /locus_tag="ACHUT9_000973"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43537..43539,aa:Val,seq:tac)
BASE COUNT        11567 a        10877 c        10127 g        11036 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgtcata
      121 gttaacgtta acatttgtga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaacaatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgccacctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagcg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gccaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat tgtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg gaccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cacaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tgttttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcggac aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacgg cgacatcctg
     1861 cgggatacgt acccccttcg ccaggagaac ctgataagcg acaaaggctg cacgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgggcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aggcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataacgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccgctgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctag
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctggcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa actgctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccagc tatgcaaaaa cggctggaaa aagaatatca
     3421 gcaaccgatc agcgggcaac tgttactgaa aaaagatagc catttgccca tttccggctc
     3481 cataaaagca cgcggcggga tttatgaagt cctggcacac gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gtcgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc aggctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc ggcactggcg ggtatggccg gacctcagcg
     4321 cgtatgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aactgcgtaa
     4381 taccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcagca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccataata
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctgaaaa aactgcggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaacta aatgtcacta acaaccgcat
     5101 gtctattttg ttgccataac gtcctatcaa aggtgaaatt aatagtggca tgatgccgat
     5161 gggcgcataa gcaagtccgg cccatatcgc attatacccc atcgtttcct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg catgtgatac tcacaatacc
     5281 aatggtgaag ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ctcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcctggtag attcattttg accggtgaag tctcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc acaaatataa ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 cggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaaccc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataggctac acattaatga
     5821 agacagcgaa aaaaaggtta ctgaaagtaa aaataatctt aattcgccta ttctttgtgc
     5881 caacctgcca gtaacaggga tcgcaatggc atttgctaca ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaatgt cgctaatgac aatgcaatag tgacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagccggcat ggaggtcacg gttgaagcca gctcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccgggac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcgcc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaggatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaagct cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc tggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacttat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgca
     8281 caacgtaaca aaatcggcta accacatgaa gtttttaccc tatatttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcatac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acataagtcc caaacggcta cgttgttgca
     8521 taccgattcg cagcaacgag ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 aacgttaaat atcaaattaa cactccggga atacgccgat catcaaaaag caatggacgc
     8641 gctggaagaa ggtgaagtcg atatagtgtt atcacattta gtagcttcgc cgcctcttaa
     8701 tgatgacatt gctgcaacca aaccactgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgattca atgcgaccgc ttacctcatc aaaaccagta aatattgctc gagtagcaaa
     8821 ttatccccct gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agccggacag aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcaccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcgttct
     9061 taatgaagta ctcaatcgat ttgttgatgc tttgacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa caatggatta agcagcatcc cgatttaaag gtgctggaaa atccttactc
     9241 ccctccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggatattct
     9301 taatattatt actttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacagctga atcccggcgg atgggatata ttacccggtg ctatttatag
     9421 tgaagataga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagcttc attcgcaatt aaaagagatg tatccggagg tagagtggat
     9601 aaaagtcgat aacgccagcg ctgcatttca caaggtcaag gaaggcgaac ttgatgctct
     9661 ggtcgcgaca cagttaaatt cacgttacat gatcgaccat tactatccta atgaacttta
     9721 tcattttctt attcccggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca atccccccaa gcgaagttct
     9841 gcgcctgacc gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctcta
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgac tcccccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 ttttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tctgccagcc agcgatcatg ctgtttatat ttgtggttgg caagatatta cagagacgcg
    10381 tgatttaatt catgcactcg aagtagagag aaataaagcg atcaatgcaa ctgtcgcaaa
    10441 aagtcagttt ctggcaacaa tgagtcacga aataagaaca ccaataagct ccattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac tccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaatttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagca atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg gttcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactcttt aaacgctaca gccaaacaag
    10981 tgcaggtcgc cagcaaacag gatctggctt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttgt cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaccc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaacgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca agcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgtttcagat gacagtaagc ctgaaattct
    11821 tcagttgctg aactctgtaa aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aataactaaa tagcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctgggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattacaaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggtcgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagcggc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgcagc agggtcgatg accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgatcatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccga atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attatttcat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atatttattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca ctggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ttcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acaaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgcgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cctaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcctatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatggttt ttatggcttt tttcttaagg ttttttcctt cgctgaatat
    20581 ctaattatta ccgtcagaaa ttaaactgtc gccgatatga gacacttgta agttactaaa
    20641 agtaaataag aaaatgcctc ttgtctcgtt tcagttttag gcgtacatta acgccgtctt
    20701 gagcagttta gctcagaatt ctggggtgat gcccacggta aaatacaggg cttttaaggt
    20761 cgctttgccc ggtgtcaact cactgtatca ggtgtaatga agtcattcag gcgtaacagt
    20821 aattacgcgg agagatgtaa agtgaaatat ttctttatgg gcatttcttt tatggtcatc
    20881 gtttgggccg gtacttttgc cctgatgatc taaaagcaaa ctgtgcaatg aaaaacagga
    20941 gccatcaggc tcctgtttgc atttctgacg acggtgttta ttccgcgttt tcgtgaatat
    21001 gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat cgctttaatc cctctgatgg
    21061 cctgacgaat acggtcgcgg ttttcaatca gtgcaaagcg aacatgcgta tcaccatagt
    21121 cgccaaagcc aatccccggc gagacacaaa ccttcgcttc gttaaggagc ttcttggcaa
    21181 attccagcga tcccatggcc gcatatggtt ccgggatttt cgcccagaca tacatcgaag
    21241 ccttcggcat ttcgaccatc cagcccgctt catgcagccc tttaaccaat acatcacggc
    21301 ggcgtttgta ctgttcggta atgtcgcgca cgcactgttg atcgccctcg agtgccgcaa
    21361 tcgccgccac ctgcaacggc gtaaaagtgc cgtaatcgtg atagcttttg attcgcgcca
    21421 gggcactgac cagcgttttg ttgccgacca taaagccgat acgccagccc gccatgttgt
    21481 agcttttcga cagcgtaaag aactcgaccg ccacatcgcg tgcacccggc acctgcataa
    21541 ttgacggcgc tttccagcca tcgtagacga tatcggcata ggccaggtca tggaccacca
    21601 gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa gaactccagt tccacgcatt
    21661 gcgcggttgg gttcgacggg aagccgagga tcatcatctt cggtttcgga taactttcac
    21721 gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc caccagcggc actgagcgta
    21781 cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg gtaacttgga ttcggcacca
    21841 gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg cgccaggccc tctttcgaac
    21901 caatagtgac gatggcttct gattccgggt cgatttcaac gtcgtagcga tcctgatacc
    21961 agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga agtggagtaa ccatgcgtgt
    22021 ccgggcgctg ggccacagta cataattttt cgacgatatg cggcggagtc gcaccgtccg
    22081 ggttacccat gctgaaatcg ataatatctt cgccgcgccg acgcgcagcc attttcagtt
    22141 cagcggtgat gttaaaaacg tagggcggga gacgatcaat gcgcgtaaag cgacgttcag
    22201 ggcgagtgtc agccataatt tcctcagatt aacgttagcg cccggaccgt ccgagcgacg
    22261 ctgccacaat ggtggctgtt ttgaaaatag cctgattaat ttctgtctgt ccagcgtttt
    22321 agaacatttt tatcaatggc tttaaatgag gaactccact tcaaaaacag gaaatttttc
    22381 tacgctaaca agaaaattac caccaatttt attaacatta taatttcaat aaatttacca
    22441 gccagaagtg cattatcaag agatacattg ctaatacacc atttatcttt caatcccctt
    22501 ttaaatttat ggtttttcct caaaaacggc ctgctggtca tcagccttca ggttacctat
    22561 catagaggtt taatccttat tcagagtcac ccgtgcacga aatattcaac atgctgctgg
    22621 cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt ctttctcatc cgtatccgcc
    22681 tgtttcgcga actgttgcac aagtcggcgc actccccaaa agaattgctc gccgttaccg
    22741 ccattttctc gctgttcgcc ctgttcagca cctggtccgg cgttcccgta gaaggctcgc
    22801 tggtgaacgt acgtattatc gcggtgatgt ccgacgggat tctgtttggc ccgtgggtag
    22861 gcatcattac cggcgtgatt gcgggtattc accggtattt aattgatatc ggcggcgtga
    22921 cggcgatccc ctgctttatc accagcattc tggcgggttg tatatcaggc tggataaacc
    22981 tgaaaatccc caaagcacag cgctggcgcg tcggtattct cggcggcatg ttgtgtgaga
    23041 cgttgaccat gattctggta attgtctggg caccaactac cgcgttgggg atcgatatcg
    23101 tctctaaaat cggcattcca atgatcctcg gtagcgtctg tatcggcttt attgtgcttc
    23161 tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc gcggcaggcc aagctggcgc
    23221 tggatatcgc caacaaaacg ctaccgctgt ttcgccatgt caatagcgag tcattacgca
    23281 aggtctgcga aattatccgc gatgacattc acgccgatgc ggtggcgatt actaataccg
    23341 atcatgtgct ggcctatgtt ggcgtgggtg aacataacta tcagaatggc gatgacttca
    23401 ttagcccgac tacccgtcag gcgatgaatt acggaaaaat catcattaaa aacaatgatg
    23461 aagcccaccg cacaccagag attcattcca tgctggtgat cccattgtgg gagaaagggg
    23521 tcgtgaccgg aacgctgaaa atttactact gccacgcgca tcagatcacc tcgtcattac
    23581 aggaaatggc ggtcggtctg tcgcaaatca tctccacgca actggaggtt tcacgcgccg
    23641 agcagctacg tgaaatggca aataaggcag agcttcgcgc cctgcaaagc aaaattaatc
    23701 cccattttct gtttaacgct ctgaacgcta tttcatcgtc aatccgtctg aatccggata
    23761 ccgctcgcca gttgatcttt aatctgtcgc gttatctgcg ctataacatt gaattaaaag
    23821 acgatgagca aatcgatatc aaaaaagagc tgtatcaaat taaagactat attgccattg
    23881 agcaggcccg ctttggtgac aagctgacgg ttatctatga tattgatgaa gaggtgaatt
    23941 gctgcattcc cagcctgctg atccagccgt tggtggagaa cgccattgtc cacggtattc
    24001 agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc agagtgcgga aatcgggtac
    24061 gcattgcggt gcgagatacc gggcacggca tcgatccaaa ggtgattgag cgggtcgaag
    24121 cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt ccatcatcgc gtgaagttat
    24181 tgtatggcga ggggctgcat atccgccgcc tggagccggg gacggaaatt gcgttttaca
    24241 ttcctaacca acgcacccca gtcgcctcac aggctacgtt attgctttga gccggagtga
    24301 tattgtgaaa gtcatcattg ttgaagacga attcctggca caacaggaac tgagctggct
    24361 aattaaagag cacagccaga tggagattgt cggcaccttt gacgacggtc tggacgtgtt
    24421 gaagtttttg cagcataacc gcgtcgacgc catttttctg gatatcaata ttccgtcgct
    24481 ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc cataaaccgt ttattgtgtt
    24541 catcaccgcg tggaaagaac atgcggtaga agcgtttgaa ctggaggcgt ttgactacat
    24601 tctcaaaccg tatcaggagt cacgtattac cgggatgctg caaaaactgg aagcggcctg
    24661 gcaacaacag cagaccagca gtacgcctgc cgcgacggta acgcgtgaga atgacaccat
    24721 taatctggtg aaagatgagc gaataatcgt cacgccaatt aacgatatct attacgccga
    24781 agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa tcctacgtaa tgccgatgaa
    24841 cattaccgaa ttttgcagca aactgccgcc gtcgcatttt ttccgctgcc atcgctcatt
    24901 ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg tttaataaca cctacattct
    24961 gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc agcaaagtga aagaatttcg
    25021 ccagttaatg catctttaat cgggaacttt catgaaagca ccaggcttgc ccgccgatca
    25081 acaatttttc gccgatctgt tcagcggcct ggtgcttaac ccgcaactac tggggcgcgt
    25141 ctggtttgcc agccagcctg cctcgttacc ggtgggcagt ttatgtattg attttccccg
    25201 tctggatatc gtgctgcgcg gcgaatacgg caacctgctg gaagcaaagc agcagcgaat
    25261 ggtggaagga gaaatgctgt ttattccggc acgcgcggct aatttaccga tcaacaacaa
    25321 accggtgatg ctgttaagcc tggtgttcgc accgacctgg cttgggttat cgttttacga
    25381 tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc cagcttccca gcctgcaacg
    25441 cggtgaaggt gaagcgatgc ttaccgccct cacccatctt agccgttcgc cgctggagca
    25501 gaatatcatt cagccactgg tgttaagttt gctgcatctt tgccgcaacg tggtgaatat
    25561 gccgccgggt aattcgcaac cgcgcggcga ttttctctat cacagcattt gtaactgggt
    25621 tcaggataat tatgcccagc cgctcacccg cgagagcgtg gcgcagtttt ttaatatcac
    25681 gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg atgcgtttta tcgagtatgt
    25741 gcgttgggtg cgaatggcga aggcgaggat gattttgcag aaatatcatc tgtcaattca
    25801 tgaagtggca cagcgttgcg gttttccgga tagcgactat ttttgtcgcg ttttccggcg
    25861 tcagtttggt ctgacgccgg gagagtacag cgcccgtttt cagggctaac gtcagaaggt
    25921 taattctgtt tccagcagcg tcaggatact ttgtgcatct tgcgcggcaa acaacgactg
    25981 gcggaagttt ttattcacca gtttacgcgc cagctgcgag aagactttca catgattcat
    26041 cccttcgtta gcacccagcg tcagcatgat caccagttca acttcgccca tttctgactg
    26101 ccagtcaacc ggtttcgcca gccgggcaat gctgatactg gaatgacgga tccactgaga
    26161 tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg gtaacaatct cttcccgctg
    26221 ccagacatct tcttccagct caaacggatg ttcagtgcgc ccgttaacgc cgaggttgcc
    26281 gcacaggaac tggatcgcct gctctttatt gctaaaatcc tgatcaacaa agatattctc
    26341 cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag gcggtgagta acgcttcaat
    26401 ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt tcccgacacg cctcgctatc
    26461 cagttgacga agctggcttt tcaccgccgg aatacgcggg ctactcatac tcagctcatc
    26521 caggcccagc ccaagcagta gcggcagata acggctttca ccgcccagtt caccgcaaat
    26581 gcctacccat ttgccccgct gatgcgcagt ggtaactatt tgctgcaaca tgcgcaggaa
    26641 cgatggcgta atcgggttat atagcggcga tacgcgcggg ttattacgat cgaccgcata
    26701 cagatactgg gtcatatcgt tggagccgat actgaagaaa tcgacctcat cggagaagtg
    26761 gtcgatgatg tagcacaccg acggcacttc caccatgatc ccaagcgtaa tcgtctctgc
    26821 atgacgcagg ccatcgcgct taagctcaac gatcgctttt tggatctcgc ctttcaccca
    26881 taagatctga tcgaggctgt gaaccatcgg gatcatcaac tgggcgttgc cgaaactggc
    26941 ggcgcgcaaa atggcccgca gttgagtgcg gaacaggcca gcaaattccg ggtaaatacg
    27001 taccgcgcga tagccgagga acgggttctc ttcctgggga atattcagat aaggaatgct
    27061 tttatcgccg ccgatgtcca tcgtgcggaa gataatcggc ttgtcgcccg ccgccagtag
    27121 cacctgctgg taggcttcaa attgctcctg ctcgtccggc gcgctgtcgc gatccatata
    27181 gagcatttcg gtacggaaca gcccgacacc ttccgcgccg ttggcaaacg cgcctggcgc
    27241 ttccagagcg gtgccgatat tcgccgcaat atcaatacgc ttgttatcac gggaataggc
    27301 cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta tccgccagcg tctgcgcgac
    27361 ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc agcacgccgc actgggcgtc
    27421 aagcactgca ggttgcccgg cataacgggc aatcgcatcc agcggcaagc cactcagtac
    27481 cgggatcgcc gaggcacggg ccagaatcag tgtatgcgag gtgcgcccgg ttttctccag
    27541 aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac tggcttgggg ttaaatcttc
    27601 agccaccaga atggtcggtt tttccagcac cagattgttg cgcggcttca gttccggcta
    27661 ggtgatatgc agcaactgtt cgctgatatc gcgaatgtcg ctgacccgct cgcgcagata
    27721 atcgctggca gaagcagata gtttggcgca aacctgctcc atattgctga tgatcgccgc
    27781 ccccagtccc tgatgctgtt ctgtcatcag gcgacggata ttgcctgcaa attcatcatc
    27841 ctgaatcagc gacaaatgag cgctgaggat agttttgctt tcgccgtcac gctcacgcaa
    27901 ttgctggttc agttgctcgg caagcgttgc caggctgtgc tccagccggg tggaatcttg
    27961 cgcactggcg gggattgccc gataactgtc gaggctgtcg ctttgtaaca gggtcagcgt
    28021 acccacgccg acgccgcttg ccagcacatt gccgtacagt aaatccgggt taaggcggct
    28081 taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc gtcggctgaa cgctgtcgct
    28141 gtcgataaag cgcaccagga tgtactcttc cagcacgcgc cgcgcctgct cttcatcgct
    28201 gccgctaatg ttcaggctgc aactgtcatt aaataaggtg ccagttccaa tcaacgccag
    28261 cgagctttta gcatcggcct ttgcgttctg gcgatggttg ataaaggtga tttcgctttg
    28321 ccactggctg cactgttctt taagttccca cgccggacga gcatgtagac cgttaggcaa
    28381 aggacagaga aattgaatcg ttaacataac cgctccgggt taatcaggag atcatctgaa
    28441 atccgtcagt tgaacaaccg tctcacgcgt aagacgttga ataagggcag ataaaagttg
    28501 ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc gcgcaatgtc cgtggcgggt
    28561 ggcaggcccc attaccactg tgggtacgcc agtatcggtt aagtgcaccg ctccaccgtc
    28621 cgtgccgccg ttactgaaca tatccgcctg caacggaacg ccaatttctg ccgacacggt
    28681 ttcaatccac gcggtaagtt ttggcggcgc aatcagcgat ttatcgctta acaccagcat
    28741 cggcccgtta ccaatctgac gatggttagc cgcgccataa tcaaagtttt tcgcccagca
    28801 ggcggtatca agcacaatgg cgacgtccgg cgacaccgcg cgggtagcag tttgcccgcc
    28861 gcgtaatccc acctcttcgc tggaacttgc caccagccac acttccgcag gcagttcggc
    28921 gtcgtgcagc tcacgtagta acgtcaccag cagatagcaa ccgaggcggt catcaaaggc
    28981 tttccccatc acccgctggt gagggagaac ctgaaaagtt gtatcaaagg tgacgcgatc
    29041 gcctggacga attcccgcct gcatcacttc gtcatacgag cgcgcaccaa tgtccacgcg
    29101 catggcgctg acgtcattcc cctgccggtc gccgtcaagc aggcctggaa ttttgcactc
    29161 ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca gccatgcgca cgttgccaac
    29221 cggcagcaca tcaattgccc cttcgcggga gatgctgcgc accataaatc ccacttcgtc
    29281 catatgcgca cagatcatca ccttcggacc tgtcgattca ttgaggcgga tcagcaccga
    29341 tcccagacca tcaaatcgta cttctttttg caggcgatcc gcttcttcca gtaggatctg
    29401 ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg ctcaacgctt ttaatagcga
    29461 taaatccatt atgcctctcc cgtgagcaac actgttttcg gcatggcgta gagcacttct
    29521 gcgccttgcg gggtaaccag cacaacatct tcgatgcgca cgcccccttg ccctggcaaa
    29581 taaatccccg gctccacggt cagtaacatg cctggctgta gcgtcgtggt atcccgcggt
    29641 gaaaagcgtg gatcttcgtg tacttcaata ccgatagcat gaccggtgtt gtgaccaaaa
    29701 tagtcgccaa atcctgcctc ggtaatcacc cggcgcgcgg cgtcgtcaac ctgttagcag
    29761 cgtacgccag ggcgaatcgc ggagattgcc gccagctgtg cttgcaggac aatctgatag
    29821 acgttaaaca gcgggtgaga ttcggcaatt accccttcgc cattcaccag caaggtgcgc
    29881 gtcatatcag agcagtaccc ctgatagagc gcaccaaaat caagagtgac aaactcgcct
    29941 gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg ccccacgcca gccgctggcg
    30001 acaatggtgt caaaagaggc tttttctgcg ccctgctggc gcataaacca ctccagttca
    30061 gcggctatct cgcgctcgct catccccgcc tgaataaagc ggcgaatatg ctccgctccg
    30121 cgatcggcaa tcccacaggc gaggcggatt atctccacct cctctggcgt tttgatttgc
    30181 cgcagcacat ccggcgtggc gctaaccagt ttcgcattga gttcagactt ccagcggtgc
    30241 gcggtttccc aactcacctg ctggccttca aaaccgagcg tttgcaactg ctcatcggca
    30301 atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt caagcaaatg cagctggtag
    30361 ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat ctaccagaat gtgcgcactt
    30421 tcacggctaa taaccacata gcctgatccg gtggagatcc ccagatgcgg ctgtttgttc
    30481 tgccgtgagg agagaagcac tgcatccagt tgttgcgcct taagccagtc gcgcagcgaa
    30541 gcgagtaatg tcatctggat tttccttctt acaggctatc gattaacaat ttacctttac
    30601 ggaacatcat cagacgcagg aacaccacca tcagcgcagt tatgactgcc cccagcgcga
    30661 tccccgccat atagacgccc aggttagtga ccagcggcca tgcccaaata gcggattccg
    30721 ggaaccattg cactgcgccc agccagacag cggcggttga gccgacaatc gcgccgacca
    30781 tatacgacgg aatggcggtg atggggcttt ccagcgcaaa cggaatcgca ccctcactaa
    30841 tccccataaa ggcaaggaac atcgcggtct taccctgcgg ataaagttga gcgttgaaca
    30901 ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag acccagacct atcggcggaa
    30961 taacgatagc gatagaacgt gcagtgaccg gtaatacgtg gtcagtggta aagctgaagg
    31021 caacaaaacc tgcggctttg ttgatcgggc caccaaggtc gattgcggtc gccgcggcga
    31081 tccccattgc gtacatcagc gcgcccttct cacctgcggc agtcagcacg gtacggatac
    31141 cgccgttgat ccagccgcca aagggggtaa tgacgtagta catcgccagc atgacaaaaa
    31201 tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc taacaggaaa tccggtaact
    31261 ggattttctg gttcatccac ttcacaaggt aacccgcgac gatagaaata atcagcgcac
    31321 caatgaaggt ggacggcacc ggcgaagagg tcgcccattg catagtgctg ggatcgaagt
    31381 tcagcagttg cgtcggctgg gtagacatca agccgccgat aaaaccagcc gggaacgcca
    31441 gtttgccacc gatagagttc gccacaaaag cagcaaacat cggaatggca aaaccaaaca
    31501 acacgccgcc gaaggactgc gacagccagg caaatttcag taaggagagg tcgaaaccgg
    31561 agaatttccc gctattaaga gcatccatga tgccgatctc agcaggaatt ttaagccagc
    31621 tataagcaat cagctggctg aaagcgagga tcacaccgcc cataattaag gtcggcacca
    31681 tgcgtgaaat ccctaacatc acatgctgcg ggagttcacc ccagaagctg cttttagagg
    31741 cctgcggatt cttaactgcc gctgccgcac cggatgcgcc aggcacaacg gttgcactgc
    31801 gttttttaat ggccataaca attatccctg tcgaatatta ctgttgttca gaggcaatca
    31861 tctcttcgat ttctttgatg atgcccgcag cgtttttaat tgcgtcctgc aaagtgattt
    31921 cataaacgtc gcgtgattcg aaacgctcat tatcttccgg ggtaactgcc acggagtgga
    31981 tgatgatggt tgcttcggcg atatcctgcg ccgtcaggcg attctggata ccgtccgcgc
    32041 cctgggtttc aattttcact tcataacctg cttctaccgc cgcttcttcc agcgcctgag
    32101 cggccataaa ggtgtgagcc aggcccattg ggcaggcaca taaggcaatt agtttcttac
    32161 tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa attatgaacg tgcaaaacaa
    32221 atcgccgtta ccagacaaat aatgcattta ctggagaatt aatcccgctg tacggaagtg
    32281 tgacgcaggt cggccttgtg agggggacac aaagaaaaat tgaattaact gtatgattta
    32341 aaagattatc gggagagtta cctcccgata taacagaaag gatttacaga atgtgcccta
    32401 aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg acgatgagat
    32461 acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat gcggcacgga
    32521 aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgccagca atataaaccc
    32581 caccaaaggt tcccagcgtt aatgccagat tgccgccaaa acggcccata atgacgcaaa
    32641 acagcgacaa cgcacggcgg caatcggtgc agctgtcagc cagcgcgcgt tcggtaatat
    32701 cttttggctt gagattttct ggcaggcggt tgtcggcttt aacaattgcg cgatacaaat
    32761 tcaccagccc aggaccagaa agcacgcgtt ccgccgaaac atgaccaatt tccgcacgca
    32821 atatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg tgaccgcctt
    32881 cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc ccaagccctg
    32941 ttccggcacc gtaaaccgca ataggtttac cttcgaccgg ttctgcgcca ccaaactgaa
    33001 tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa tcgttaataa
    33061 tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac gcccaggtat
    33121 ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata cagccgtctt
    33181 tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg ctggggtaat
    33241 caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca cacagagcaa
    33301 gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt cttcaactgc
    33361 tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca taacaacaac
    33421 gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact ttatcgtttc
    33481 agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct gaccccgcat
    33541 aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc gctccccgcc
    33601 gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat cgcctcggta
    33661 ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca ggattcacct
    33721 atctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt tatccgtttc
    33781 agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc accagttccg
    33841 ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg cggcgtcagc
    33901 ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgccgtcgc cattggcgct
    33961 cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc cggaaccatc
    34021 ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac cttaaatggc
    34081 agtagtgaag ttccgctatg ggatcgtctt tttgcgccgt taatggcggc agcagctggt
    34141 gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc gcattacgga
    34201 cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc catcgcagca
    34261 gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca aatgaaaaat
    34321 ccggtgctcg tgctgggtat tggcggattt attctcggaa ttctgggggt tattggtgga
    34381 ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa tcaggctttc
    34441 agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt cgttgctgcc
    34501 gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt ggcattaggg
    34561 ttgatgctgc atgagcacgt tcccgccgtt ccagcggcaa taaccgtttc ctgcgctatt
    34621 ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat ggcggcagtc
    34681 gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc ggcatggctg
    34741 ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc accccacgat
    34801 aatgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg atacggggct
    34861 ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg aaggagaata
    34921 acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc gcttgcagca
    34981 aatgcaggtg aaatcaccct actgccatcg atcaaattac aaattggcga tcgcgatcat
    35041 tacggtaatt actgggacgg tggtcactgg cgagatcgtg attactggca tcgcaattat
    35101 gaatggcgca aaaaccgctg gtggcgtcat gataatggct atcatcgtgg ctgggataag
    35161 cgtaaagcat atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga tcatcgcgga
    35221 aaaggccgcg gacatgggca ccgccattaa acgcgtcctc caatggagca caatgcctga
    35281 tgcgacgcta gcgcgtctta tcaggcctac aaaccggctc tttaacgcgt agggcggata
    35341 aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa ctacaatccc
    35401 agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac aatcacccag
    35461 cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct gtcactggtg
    35521 aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac ctgactcata
    35581 accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg caacatggtg
    35641 actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat caccacctgc
    35701 cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact taacccaaag
    35761 accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc atcaagatcg
    35821 gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat catcgccaga
    35881 ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc ggaataacgt
    35941 tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata aatcacatgc
    36001 ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcctccga agtaggtaaa
    36061 ctcgggatca ccattccttt acccagctgc gccaggttag gctgggagaa aatcaactcg
    36121 acaatgtaag ccgcggcaac aaacaacagt aacccgccaa tcactttctc cagtggtttt
    36181 tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag caccgcgccc
    36241 tgcaacaacg agacaccaag aatgagttta aaaccgatcg ccgcaccaat aaattccgcc
    36301 aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac tacgggacgc
    36361 ggatagtgat cacgaatctg ctccgccaga ttttttccgg tggcaatccc tagtttggca
    36421 gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca cagtagctga
    36481 tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt taccgggatc gatataacca
    36541 atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc cgcccgtccg
    36601 ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc ctttgctatg
    36661 tttcatgcta tgataaacga gaatgattat caaattcatt taaatggatt gtggtgattt
    36721 ctctgataga ccaggattat gactacgaaa agattgatga cttgaatgtt atggttatgt
    36781 ttaatgttag cacatttaca taactttcag cttccataca caacatagca gaaatgtatg
    36841 acagatcact atttttgaag cctgtcacag gacgtcatta tagtgtgtgt cagatctcgt
    36901 tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca tatttggagc
    36961 aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga ttctcgcact
    37021 gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac tgcttgttat
    37081 cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct tcgtgaaagg
    37141 cttctccgaa atgttcgaaa aactgctcag atttgccaac gaagggacta acttcgtctt
    37201 tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt gcccaatcgt
    37261 ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg tgattatccg
    37321 cgcaattggt ttcctgctct ccaaagtcaa cggtatgggc aaactggaat cctttaacgc
    37381 cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag atatcctcgg
    37441 caaaatctcc cgtaatcgta tgtacaccat ggctgccacg gcaatgtcca ccgtgtcgat
    37501 gtccatcgtt ggtgcataca tgaccatgct ggaaccgaaa tacgtcgttg ctgcgctggt
    37561 actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aatccttacc gtgttgatgc
    37621 cagtgaagaa aacatccaga tgtccaacct gcacgaaggt cagagcttct tcgaaatgct
    37681 gggtgaatac attctggcag gtttcaaagt tgccattatc gttgccgcga tgctgatcgg
    37741 ctttatcgcc ctgatcgccg cgctgaacgc actgtttgcc accgttactg gctggtttgg
    37801 ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg catgggtgat
    37861 gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca aactggtttc
    37921 caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct ctccgcgtgc
    37981 tgaaggcatc atctctgtgt tcctggtttc cttcgctaac ttctcttcaa tcgggattat
    38041 cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc gcttcggtct
    38101 gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg cagcactggt
    38161 gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta cgcctgttaa
    38221 tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac cccgagctta
    38281 tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact caatgacttc
    38341 gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtatt cgtgacaata
    38401 tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt taaccgttcg
    38461 tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa cttatgcagc
    38521 tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc ggtgatctca
    38581 agaatgaccg cctgcgggga gatgtgataa cgcttaaaca gacgaataat ccgcccggca
    38641 atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg tccttttttg
    38701 tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa atcaaaacgc
    38761 gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat aatgccgccg
    38821 tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt gcgaattggc
    38881 tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt cagcaatagc
    38941 acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc cagcacatga
    39001 tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg ttgcagggtt
    39061 tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag cccggtattg
    39121 ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg cccttccgtt
    39181 tccggcccgc tcagcactaa cagcagttca ctacccggca attgatataa cttttcgttt
    39241 tcctgcatca acggcagcag cgtacggtaa attgatcgga tacagtgaac gcgcatcatt
    39301 aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca aaactcttgc
    39361 ccgcctcttg ctccggcgct tgttccaacg cacggaagtt gggcaggagc gttaacggat
    39421 cggtcagcgc ctgcaaatgc cagcggcgat taagccattc actgcgatga taaatgcgca
    39481 ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc aagaataaat gccagcgaat
    39541 attcggtttc caccccttgc aaaaagttct ggttgtaatt cagaaggcaa agcgttgaaa
    39601 cagcccaggt gagatttaaa aacggatagc gaagcttacc gaccccgagg gtaaagatga
    39661 tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt ttcataaggt gtgcacaaca
    39721 gaagtagaag cacgcttaac gctgccagcc agcttaaggt aaatgcgcgt ttctctttgc
    39781 ccaacgacgg cgcgatatcc ctgcgccaca atatctgcgc aaagtgggga cttacaatca
    39841 tgcgagtgag atagtagaaa agcatgttgt aaataagcac agcggtgaaa aggcttagca
    39901 aatcaacgac cgtgaaaatg gcatccgcat cgccgaaaaa ggtggatatc tttagcggga
    39961 aatcaaagaa gctccccaca agatacatgc tgcatttgat gccgatcggc gtcaccaaac
    40021 caagccagaa cagacgttgc cagacatatc ggctggtcaa tccataacgc caacgcgtcc
    40081 ccagctgcca gcgtaatatt gcgcaggcac acaacacgac aaacgtctgg ctaccgagca
    40141 tgaccgcagt ctgtaaaaaa gagagatgaa agttccacag attggtgcaa aacatcccgc
    40201 ataaaacggg aacgacaccg cgccagccaa agataaacaa gattgacagc attacgcaca
    40261 gtggcatcca cgccagaaag atataactgg aatggataat ggcaagtggc gaaataaaac
    40321 gggatagctg aataagtacc acggtgagcg taaacgctag tgtgaatatc ttgatatttt
    40381 ttatcaggtt atgctccaca aacatgagta cactactctc actgatcggg gttttcaggc
    40441 acacaaataa taagtttatt cgcatcgcga agcaaacagg gggccagcct gttgccgatt
    40501 tttcatccaa tgatgctttc accaggcggc actaacccgg acgtagactg tattacaaaa
    40561 gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa atttggtgga
    40621 gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc ccagctgagc
    40681 tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg gtggagctaa
    40741 gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc tgagctatag
    40801 ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc gcccgaatgt
    40861 gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa atgaacaatt
    40921 ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa agtggtgtta
    40981 taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc aaggagagga
    41041 tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt aataaatggg
    41101 tacgcaagga gggctggaca acgtcaccaa aacctggcgt ccagggtggc aaagcccgac
    41161 tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt ccagaaggtc
    41221 agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg gtgacactgg
    41281 cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg cgggaaggga
    41341 ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa aagattacgc
    41401 aataaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca aactgttaac
    41461 cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa aggcggtcgt
    41521 gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac gccagccttc
    41581 cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc tcctggagca
    41641 cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga cattgagcag
    41701 ggaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc ccgtctcgat
    41761 attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt ttatcttttt
    41821 acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt tgttgatacg
    41881 ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg gagactgccc
    41941 cgccccggtt acggcgacac gcagcggcat accaacttta cccataccca cttccagctc
    42001 atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag taatcgcggc
    42061 cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat gttttttcgc
    42121 cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct gtgccatctc
    42181 tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct gcgggccgtt
    42241 acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa catactccgg
    42301 cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt tgaacgcact
    42361 ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac gagtgaagat
    42421 ttcctgatcg ccgtgggacc agcccagacg caccagatag ttcagcagtg cttctggcaa
    42481 ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg acagtttttt
    42541 accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg ctttcagggc
    42601 cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac ggataacgtg
    42661 ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg gggaaccatc
    42721 ggtacggcgg ataataagat cgtccagttc ctggttgctg aactcgatcg gaccacggat
    42781 ctgatcgtca aaaacaacag aaccttcctg cgggttagca aaacgtacaa cacacggttc
    42841 atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg gcttctcacc
    42901 tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat agcatttata
    42961 agcagtgccc tcttccagca tctgatcgat caccgcgttg tagcgatcaa aacgtttggt
    43021 ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat ccataatggc
    43081 ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca gcacgaactc
    43141 accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc cgccaacgtg
    43201 cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat ggccttacgt
    43261 ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat attctatcac
    43321 tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg ttttcgttga
    43381 caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga cgaacgaaca
    43441 atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcgg ctccacacag
    43501 cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt cggcggttcg
    43561 atcccgtcat cacccaccaa ctactttatg tagtctccgc cgtgtag
//
LOCUS       DAZPEP010000018        43266 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZPEP010000018 DAZPEP010000000
VERSION     DAZPEP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.018"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            50..568
                     /locus_tag="ACHUT9_000974"
     CDS             50..568
                     /locus_tag="ACHUT9_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFR1347513.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            584..1123
                     /locus_tag="ACHUT9_000975"
     CDS             584..1123
                     /locus_tag="ACHUT9_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFR1347514.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1218..2795)
                     /gene="guaA"
                     /locus_tag="ACHUT9_000976"
     CDS             complement(1218..2795)
                     /gene="guaA"
                     /locus_tag="ACHUT9_000976"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFR1347515.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2864..4330)
                     /gene="guaB"
                     /locus_tag="ACHUT9_000977"
     CDS             complement(2864..4330)
                     /gene="guaB"
                     /locus_tag="ACHUT9_000977"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFR1347516.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4492..5862
                     /gene="xseA"
                     /locus_tag="ACHUT9_000978"
     CDS             4492..5862
                     /gene="xseA"
                     /locus_tag="ACHUT9_000978"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFR1347517.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5859..6074)
                     /locus_tag="ACHUT9_000979"
     CDS             complement(5859..6074)
                     /locus_tag="ACHUT9_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFR1347518.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6143..7615)
                     /gene="der"
                     /locus_tag="ACHUT9_000980"
     CDS             complement(6143..7615)
                     /gene="der"
                     /locus_tag="ACHUT9_000980"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFR1347519.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7733..8911)
                     /gene="bamB"
                     /locus_tag="ACHUT9_000981"
     CDS             complement(7733..8911)
                     /gene="bamB"
                     /locus_tag="ACHUT9_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFR1347520.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8922..9542)
                     /gene="yfgM"
                     /locus_tag="ACHUT9_000982"
     CDS             complement(8922..9542)
                     /gene="yfgM"
                     /locus_tag="ACHUT9_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFR1347521.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9560..10834)
                     /gene="hisS"
                     /locus_tag="ACHUT9_000983"
     CDS             complement(9560..10834)
                     /gene="hisS"
                     /locus_tag="ACHUT9_000983"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFR1347522.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10945..12063)
                     /gene="ispG"
                     /locus_tag="ACHUT9_000984"
                     /gene_synonym="gcpE"
     CDS             complement(10945..12063)
                     /gene="ispG"
                     /locus_tag="ACHUT9_000984"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFR1347523.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12090..13103)
                     /gene="rodZ"
                     /locus_tag="ACHUT9_000985"
     CDS             complement(12090..13103)
                     /gene="rodZ"
                     /locus_tag="ACHUT9_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFR1347524.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13388..14542)
                     /locus_tag="ACHUT9_000986"
     CDS             complement(13388..14542)
                     /locus_tag="ACHUT9_000986"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFR1347525.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14692..15123)
                     /gene="ndk"
                     /locus_tag="ACHUT9_000987"
     CDS             complement(14692..15123)
                     /gene="ndk"
                     /locus_tag="ACHUT9_000987"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFR1347526.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15272..17584)
                     /gene="pbpC"
                     /locus_tag="ACHUT9_000988"
     CDS             complement(15272..17584)
                     /gene="pbpC"
                     /locus_tag="ACHUT9_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFR1347527.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17585..22546)
                     /gene="yfhM"
                     /locus_tag="ACHUT9_000989"
     CDS             complement(17585..22546)
                     /gene="yfhM"
                     /locus_tag="ACHUT9_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFR1347528.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22753..23598
                     /gene="sseA"
                     /locus_tag="ACHUT9_000990"
     CDS             22753..23598
                     /gene="sseA"
                     /locus_tag="ACHUT9_000990"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFR1347529.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23934..24059
                     /gene="timP"
                     /locus_tag="ACHUT9_000991"
     CDS             23934..24059
                     /gene="timP"
                     /locus_tag="ACHUT9_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFR1347530.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24091..24867)
                     /gene="sseB"
                     /locus_tag="ACHUT9_000992"
     CDS             complement(24091..24867)
                     /gene="sseB"
                     /locus_tag="ACHUT9_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFR1347531.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25009..26292)
                     /gene="pepB"
                     /locus_tag="ACHUT9_000993"
     CDS             complement(25009..26292)
                     /gene="pepB"
                     /locus_tag="ACHUT9_000993"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFR1347532.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26470..26670)
                     /gene="iscX"
                     /locus_tag="ACHUT9_000994"
     CDS             complement(26470..26670)
                     /gene="iscX"
                     /locus_tag="ACHUT9_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFR1347533.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26682..27017)
                     /gene="fdx"
                     /locus_tag="ACHUT9_000995"
     CDS             complement(26682..27017)
                     /gene="fdx"
                     /locus_tag="ACHUT9_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFR1347534.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27019..28869)
                     /gene="hscA"
                     /locus_tag="ACHUT9_000996"
     CDS             complement(27019..28869)
                     /gene="hscA"
                     /locus_tag="ACHUT9_000996"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFR1347535.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28886..29401)
                     /gene="hscB"
                     /locus_tag="ACHUT9_000997"
     CDS             complement(28886..29401)
                     /gene="hscB"
                     /locus_tag="ACHUT9_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFR1347536.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29497..29820)
                     /gene="iscA"
                     /locus_tag="ACHUT9_000998"
     CDS             complement(29497..29820)
                     /gene="iscA"
                     /locus_tag="ACHUT9_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFR1347537.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29837..30223)
                     /gene="iscU"
                     /locus_tag="ACHUT9_000999"
     CDS             complement(29837..30223)
                     /gene="iscU"
                     /locus_tag="ACHUT9_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFR1347538.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30251..31465)
                     /gene="iscS"
                     /locus_tag="ACHUT9_001000"
     CDS             complement(30251..31465)
                     /gene="iscS"
                     /locus_tag="ACHUT9_001000"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFR1347539.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31706..32194)
                     /gene="iscR"
                     /locus_tag="ACHUT9_001001"
     CDS             complement(31706..32194)
                     /gene="iscR"
                     /locus_tag="ACHUT9_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFR1347540.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32495..33232)
                     /gene="trmJ"
                     /locus_tag="ACHUT9_001002"
     CDS             complement(32495..33232)
                     /gene="trmJ"
                     /locus_tag="ACHUT9_001002"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFR1347541.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33351..34154
                     /gene="suhB"
                     /locus_tag="ACHUT9_001003"
     CDS             33351..34154
                     /gene="suhB"
                     /locus_tag="ACHUT9_001003"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFR1347542.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34299..35153
                     /locus_tag="ACHUT9_001004"
     CDS             34299..35153
                     /locus_tag="ACHUT9_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1347543.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35344..36624
                     /gene="csiE"
                     /locus_tag="ACHUT9_001005"
     CDS             35344..36624
                     /gene="csiE"
                     /locus_tag="ACHUT9_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFR1347544.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36616..37755)
                     /locus_tag="ACHUT9_001006"
     CDS             complement(36616..37755)
                     /locus_tag="ACHUT9_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFR1347545.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     SAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37915..38805)
                     /gene="hcaR"
                     /locus_tag="ACHUT9_001007"
     CDS             complement(37915..38805)
                     /gene="hcaR"
                     /locus_tag="ACHUT9_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFR1347546.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38941..40302
                     /gene="hcaE"
                     /locus_tag="ACHUT9_001008"
     CDS             38941..40302
                     /gene="hcaE"
                     /locus_tag="ACHUT9_001008"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFR1347547.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40299..40817
                     /gene="hcaF"
                     /locus_tag="ACHUT9_001009"
     CDS             40299..40817
                     /gene="hcaF"
                     /locus_tag="ACHUT9_001009"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFR1347548.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40817..41137
                     /locus_tag="ACHUT9_001010"
     CDS             40817..41137
                     /locus_tag="ACHUT9_001010"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFR1347549.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41134..41946
                     /gene="hcaB"
                     /locus_tag="ACHUT9_001011"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41134..41946
                     /gene="hcaB"
                     /locus_tag="ACHUT9_001011"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFR1347550.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41956..43158
                     /gene="hcaD"
                     /locus_tag="ACHUT9_001012"
     CDS             41956..43158
                     /gene="hcaD"
                     /locus_tag="ACHUT9_001012"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFR1347551.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10264 a        11948 c        10863 g        10191 t
ORIGIN      
        1 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
       61 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
      121 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
      181 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
      241 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
      301 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
      361 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
      421 tttaacatat aaggaaggca ccaaagtgta tacctccacc caggtgggta aagagtgcca
      481 gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc
      541 aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
      601 ttgcgccatc ttagcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
      661 ctctgctgtc gcagaagcgg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
      721 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
      781 ggccgccgct aacaaaagag cggcaacggc agcggcaaat aagaaagctc gtcaggataa
      841 actggacgcc gaagccactg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
      901 cagcttagag attcagaaac aaaaactgac gctggcgaaa gaagaagccc gcgttaagcg
      961 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
     1021 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
     1081 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
     1141 aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
     1201 tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca
     1261 tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg
     1321 aaatcgtacg gcagacgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc
     1381 agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga acgtaccggc
     1441 aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca
     1501 atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccagc
     1561 acacgaacgc caaggcctgg tcccgggaat gggtgacggt acaacatgtc gtacggcagg
     1621 cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc
     1681 aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt
     1741 gctttaccgg ttgcagacgc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc
     1801 agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcga
     1861 ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga
     1921 tcttctgccg gaacgtgaac aatgttcaga ccaaagtgat cgccaaacat atccagaacc
     1981 tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta
     2041 ccgatagcgc ggtgcagtag cattgcggtt acggaggaat ccacgccacc agagaggccg
     2101 aggatgactt tatcgtcgcc tacctgctca cggatgcggg ctacagcatc gtcgataatt
     2161 ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg
     2221 cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt
     2281 tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg
     2341 gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggtttaccg
     2401 tctgcggtca gcgcatcttc gataccgcga accagtgcac tgtcgtttac gacttcaacc
     2461 tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc
     2521 atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc
     2581 ggcgcacgcg ggctgttttc ttcagtagta ctttccgggc cgccggaaag aataatgccg
     2641 cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa
     2701 acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtccaga
     2761 atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa
     2821 acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt
     2881 agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac
     2941 cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca
     3001 gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt
     3061 tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct
     3121 ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag
     3181 atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca
     3241 gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg
     3301 agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg
     3361 cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga
     3421 tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg
     3481 cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg
     3541 tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa
     3601 cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa
     3661 ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg
     3721 ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca
     3781 gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt
     3841 cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt
     3901 cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa
     3961 cgaccggata gcccgcaaaa ccgttacgct cggtcagttc tttcacttcg cgcagcgtcg
     4021 tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc
     4081 gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt
     4141 cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca
     4201 tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca
     4261 gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga
     4321 tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac
     4381 agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc
     4441 gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct
     4501 tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt
     4561 gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct
     4621 tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc
     4681 cgcaacagca accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc
     4741 gccaatatta cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag
     4801 ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct
     4861 gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt
     4921 gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc
     4981 gatccttctc taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg
     5041 caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt
     5101 gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg
     5161 gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc
     5221 attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg
     5281 agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg
     5341 gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta
     5401 cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa
     5461 aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg
     5521 ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc
     5581 attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt
     5641 gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg
     5701 cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt
     5761 aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagttaaa
     5821 aacatccagc cagtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg
     5881 cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct
     5941 tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc
     6001 ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga
     6061 caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg
     6121 cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac
     6181 gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt
     6241 ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca
     6301 tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa
     6361 tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca
     6421 gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca
     6481 cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac
     6541 tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt
     6601 ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca
     6661 caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg
     6721 aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggtatcttca atggcctgca
     6781 acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc
     6841 cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg tagatactgt
     6901 cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag
     6961 tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt
     7021 gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc
     7081 agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca
     7141 gcacacgctc cagcagactt aatacgccac gaccgtgaga tgcggcgatc gggtagattt
     7201 cacctaaacc aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt
     7261 tgtttgccac caggaaggtc ggtttttcac gagagcgcag atgtttggca atcgcttcat
     7321 ctgccggcat caagcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa
     7381 tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc
     7441 cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt
     7501 cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa
     7561 ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta
     7621 aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac
     7681 aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg
     7741 taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg
     7801 gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga
     7861 cgttaatcca gtgcagataa ccttcactgt cacctaccac caggttgcca ttatacagca
     7921 ccggcgaagt cagcaggcga tgcagcagat cgctttgtgt ccacagcgta acgccgccat
     7981 caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga ttgccgtcga
     8041 cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat
     8101 caagcgccgt caggttacca ttataggcca gagcgaaaac aacgccgtta acaacgacgg
     8161 gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac
     8221 gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc
     8281 cccccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg
     8341 aaggcatatc gaggttaact gtccatttga cagcgccgtc agcttcgttc agcgcttgta
     8401 actgaccgtt actagtgtgg attaacacca gaccgtcgct gaccaccggg cgagaaagtg
     8461 cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa
     8521 cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaca ccgccagaaa
     8581 gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt
     8641 ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa
     8701 cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg
     8761 aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct
     8821 ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca
     8881 gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta
     8941 attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc
     9001 catgcactac gcgcaccttg cttatcacct ttgctcaaca atgctttacc acgcaggtcg
     9061 gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca
     9121 gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt
     9181 tcatcactcg tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt
     9241 ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt
     9301 tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta
     9361 accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag
     9421 tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca
     9481 ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc
     9541 acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct
     9601 gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca
     9661 cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact
     9721 gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac
     9781 gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt
     9841 atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgtact aacaatacaa
     9901 gacgttcgag gcccatcgca aaaccgactg ccggtgttgc acgaccgccc agttgttcca
     9961 ccagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgagg ctgttagtca
    10021 cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg
    10081 tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt
    10141 cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat
    10201 tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca
    10261 gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt
    10321 ccagcgaacc aatagagttc agctcaagag ttacgtgttc gaagataccc agcgcacgcc
    10381 accagcgggc agtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa
    10441 cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga
    10501 acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc
    10561 cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat
    10621 cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga
    10681 atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca
    10741 cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt
    10801 tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa
    10861 tgaaccctca acgcttccct caatgtttcg ggagccatgc gggttcaatc atacacggga
    10921 agcgaggcgc ttcccatcac gttattattt ttcaacctgc tgaacgtcaa ttcgacgcgc
    10981 ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt
    11041 gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc
    11101 ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat
    11161 cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc
    11221 gataacatca aattcctgac gcgaacaggt cgggcaggcg atgaagttaa tccctcgcga
    11281 acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc
    11341 cgccagcgat acgcgcagcg tgtcgccgat gccttccgac agcagcagac ctaaaccaat
    11401 ggcggatttt actgccccgc tgcgtgcacc accggcttcg gtgatcccca gatgcagcgg
    11461 ctgatcgatc tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc
    11521 tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa catgacgcat
    11581 ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc
    11641 cagcgatccg gcgttaacgc caatacggat cggaatgttt ttatcgcgcg cacagtcaac
    11701 caccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac
    11761 gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg
    11821 cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga
    11881 gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc
    11941 gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat
    12001 cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg
    12061 catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg
    12121 gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga
    12181 tactggatct gtactgcggc tggcgcacca attttcagtt tatacggtgc ctggccggtt
    12241 aaattcaaat taccgtcttt acgctgcatc ccgctaaaca attttttacc ggtagcatca
    12301 gtgacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc
    12361 ggcgtggtca cgccagcctg atcggttggc aacggcgcag caccatctgg cgttgttgct
    12421 gccgttggta ccggggtcgc ggcggtatca acatttgcct gcgaaggcga aacaaccgca
    12481 ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt
    12541 gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac
    12601 gtatttaacg ggacactctg cccctgctcg ctattactgc tcagttctgc cgaagattga
    12661 tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg
    12721 ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca gccatcacgt
    12781 tttttgcggc gtttaccgag ggaaaaactc tgcattggcg caacttttgc agcccgtagt
    12841 ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca
    12901 taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct
    12961 tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta
    13021 agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg
    13081 tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg
    13141 gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag
    13201 tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg
    13261 catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt
    13321 gcaacttaat acttcattca taaaacatta cgccacggta cataaagtaa ccgtggcgta
    13381 atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac
    13441 gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatca atatcatcac
    13501 cacgagtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt
    13561 cgatacggct gttcgagctg cgtccatacg gcgcacccgg gaacgggttc cacgggatca
    13621 ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc
    13681 cgtcgttaac gtggtcaagc atcacgtatt caatagtgac tcggccctga ttggcgttgg
    13741 atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga
    13801 tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa
    13861 cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga
    13921 cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga
    13981 cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattggcc
    14041 gctgaccggt gacttttgct gcgccgacga ttttcgccgc ccgccacacc tggccgataa
    14101 tttccgacac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact
    14161 ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat
    14221 acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg
    14281 aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc
    14341 gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc
    14401 acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca
    14461 tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag
    14521 gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct
    14581 ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt
    14641 acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt
    14701 gcgcgggcac acttcgcctt cgccgaagaa ataagcgatt tcgcgagcgg cagattcgac
    14761 ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt
    14821 accggccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgaacggc
    14881 gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc
    14941 atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgagcctgtt caacggtcag
    15001 gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt
    15061 accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc
    15121 catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga
    15181 ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat
    15241 gagttttagc aacaatcagg gacaaaacag actattgcat gacaaatttc actgtcgcga
    15301 tttgccccac gtcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg
    15361 tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg
    15421 cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa
    15481 tcgcgccatc gcgaacgcct gtcagctgca gagggagctg ggcatcgtga ccgtatggcg
    15541 ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg
    15601 gttccagcgg tagcggccag acatttacca tttcttgctt cgcctgcggg cagtcagcgg
    15661 cgacgcgctg ccctttgtca tccagccaga tggggaagcg aatgccatta atcccttcct
    15721 gctctgacag taacagcgtt ggcggctggc tgccatccag cagccaggtt gccaggcggc
    15781 ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac
    15841 gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat
    15901 tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg
    15961 tgccgtcagg tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc
    16021 aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca
    16081 aggcactatc cggcaagggt tgcgcttcat cagccataat ccggcgaatg atccacgccg
    16141 cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc
    16201 ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac
    16261 cagcgccgcc gagaatgagt gaaagattcg gcgcagcgcc gttgggcaaa tataacggca
    16321 atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca
    16381 cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac
    16441 cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg
    16501 atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg
    16561 atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga
    16621 gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg
    16681 ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc
    16741 gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca
    16801 tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt
    16861 cccttgactc ttttacctgc tcacgggacc acacaccttg cgcagccatc cgttcgagca
    16921 ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg
    16981 cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc
    17041 cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt
    17101 taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc
    17161 agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct
    17221 gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg
    17281 cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat
    17341 tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga
    17401 tgccgtcagc atcggcgaag cgccagagcg gcgtaccatc ctgcgccacg accactcgtg
    17461 cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa
    17521 aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag
    17581 gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc cattggggaa
    17641 catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg
    17701 ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat
    17761 cgtcacggaa ctcaatgtgc ttaatactcg cctgctgcat ctggttcagt aagttctgca
    17821 cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctgg ttttccagtt
    17881 ccagacccac aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta
    17941 cctgcaacca caccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac
    18001 catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg
    18061 attgcggata accgctggca tccatacgca accataacgg ctgatcgcca ctgttgctca
    18121 cctgcaaggt gacaagttga tcgctattca gattgctgtt ttgcgctttc tcgcctgtca
    18181 gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac
    18241 gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaacgcct
    18301 gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg tagcagttta ttttcttcca
    18361 gcaaggagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc
    18421 gctcatcact atttctcggc gttttcagcg ccagcgcaat cgcttcttca ccacgcgtcg
    18481 catcacccat ggttttcagc gcaatgccaa gttgcagcag cggtaaacca gaagcggcat
    18541 ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca
    18601 gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg
    18661 ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa
    18721 tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt
    18781 aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat
    18841 cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat
    18901 cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga
    18961 acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat
    19021 aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag
    19081 gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg
    19141 taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat
    19201 gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc
    19261 catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg
    19321 ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg
    19381 caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg
    19441 tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta
    19501 tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca
    19561 attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg
    19621 ttaccgacag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac
    19681 gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa
    19741 taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct
    19801 gccacggatc tggcgtaacg tagtcagtaa tattcagcac accactatca acggcagaca
    19861 ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg
    19921 ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt
    19981 tttcatcacc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag
    20041 atttatcgcc aggacgcacc accagcgtac tcaaataaag atcatgacga ttccaggttt
    20101 tatcgaccgg aatcgtcaga tccagccctt gagccggaac atcaatctct tgccaccaca
    20161 gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc gcggcaatat
    20221 gcaacttaat ggtatcgccg gggcgataac tggctttatc tagtttcagc gtgacacggt
    20281 cgggtcgcac tgcgccgctc ccgtcgctgt tgtcctgcca gctatagcca gcccaaaaac
    20341 gcacgctact gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta
    20401 ccgggaaact gaccttaccg gtttcatccg cctgaagatc cagagtttgt tcattttcga
    20461 tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt
    20521 aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt
    20581 gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt
    20641 tcaccgtgct gtcagtgcga taatcgtaaa ccgatttcaa ggcgaactgc ggacggagcc
    20701 ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc
    20761 ccgattccag caaactgccc tggaaaataa cctgcaatgg ggaatgcgtt tccttccact
    20821 ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga acttcatcca
    20881 gcgtgcggga aaggttttcg gcagctatat cgccgaattc aaaaccaggt aaggctgaca
    20941 cagcctcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca ttagcaggcg
    21001 caccgtacag gtagtatccc accacggaga atttcacttc atcattcggc gtaagcgggg
    21061 ttttctcacc ggtcagattc agcgccatac gctctggcat aaaatcttcg acgtggaaat
    21121 cccacatccg gtactggtta tcgcccgtgt tggcgcgaat atgccacata ccggttgccg
    21181 cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga ctaacgacgt
    21241 tcctgagtac ctgcccgtca ggtttaatca cgtctaactt gatgggttga tcgggcaatg
    21301 ctttaccgtc tgcatcgcgc agtaaaccat tgaggattac cgtttcacct gggcgataaa
    21361 gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa
    21421 attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga ccgtctttac
    21481 gtgccagcag taatgccgca tttttatcat tttccagctg cacatgcccc tgagcgtcac
    21541 tggttgcctg agtcagagtc tgtcctttct catttaataa agagacttca attccttgct
    21601 gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga taacggtgag
    21661 ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc
    21721 cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg atatcgccca
    21781 gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt
    21841 agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc gaattgcggt
    21901 attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag aagttaacat
    21961 cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg ccaggcagca
    22021 gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt atagtttttt
    22081 cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca atagtaacga
    22141 tcaaatcacg tttcggttcg aggtggcgta aacgtagctc tttaagatta tctgacagct
    22201 cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg cgtgagaaat
    22261 cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca ccatcaagtt
    22321 ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg gccagttttt
    22381 gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa
    22441 cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg
    22501 ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt catccctgcc
    22561 acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg ttatgtaatt
    22621 cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac agccaaatca
    22681 gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac cccggcaaaa
    22741 atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc cgaacatatt
    22801 gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca ggaagatcgt
    22861 aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt tgatatcgaa
    22921 gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc
    22981 gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta tgacgaaggt
    23041 aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt agagaaagtg
    23101 tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt
    23161 gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc cgtggtgaaa
    23221 gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga tgcccgcccg
    23281 gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg cggacatatt
    23341 cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc
    23401 gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc
    23461 agctgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac gctggatgtg
    23521 ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg
    23581 gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt tggcggtttt
    23641 ttattgctgg tctggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg
    23701 cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa agagagttat
    23761 tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca agcgggcgct
    23821 ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg
    23881 cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga
    23941 tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa gtaaataata
    24001 accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc agcaagtaag
    24061 cccgaatgcc gccctttagg gcggcatatt ttagattatc cggttttgtt taaagtcacg
    24121 caaaaaacca ccccagcgac gttcatagaa tggcacaata tgttcggtaa taaagtggct
    24181 aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc caggtaatgt
    24241 atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat cggcttcaat
    24301 gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa taaaagcacg
    24361 cttcaccggc ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg
    24421 ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct ggctgctcag
    24481 cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt ctttaccggt
    24541 cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac
    24601 gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt
    24661 gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcact
    24721 atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag
    24781 tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc
    24841 ttcaagttcg ttttttgttt cggacataag aaccacaatt cattcaacgt tcggggcgca
    24901 aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt tgaatttgca
    24961 gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa
    25021 cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact gttcaaccgg
    25081 cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac
    25141 aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt
    25201 attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg ccagcggcag
    25261 acgccagaat ggttcgttct cttgtgaggc actcgccagc aaacgaccgg caagcgcatc
    25321 gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg ccccggtgag
    25381 ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca gaccatcggc
    25441 aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac cgttgcgata
    25501 ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg cacagcagag
    25561 gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac
    25621 cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct gtttgatgct
    25681 gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca ctggcgcttc
    25741 tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg
    25801 tccgacggtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg
    25861 ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct gtgccagttg
    25921 cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat
    25981 caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact ccactttacg
    26041 cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc
    26101 ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg
    26161 gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat tattaatgct
    26221 gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat
    26281 cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc agccccagga
    26341 tttttccgct aaatgattcg cgttgcagaa aggcggcaag tgagtgaagc cccaggagca
    26401 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
    26461 acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga ggattttctc
    26521 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
    26581 ggtgaatcga accgttttcg gatcaagatc aggatacgca tcgtacagtg cttcgccaat
    26641 ttcgcggcta tcggtccact taagtcccat actaacctct gttaatgctc acgcgcatgg
    26701 ttgatagtgt aacgcgggat ttcgactact aaatcttcgt cggtaacgcg tgcctggcag
    26761 cttaaacggc tttcgggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
    26821 gagctttctg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    26881 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    26941 gtttcaccgc tattagcttc cagaacagcg ccatcaggac agagatcctg atgaggcaaa
    27001 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    27061 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    27121 ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag cggcagcatc
    27181 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    27241 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    27301 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    27361 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    27421 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccaccagc
    27481 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    27541 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    27601 gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt tctccaccag
    27661 gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca gcatttcgct
    27721 gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    27781 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    27841 cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcgt cagcttctac
    27901 acccgcgtct ttaagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
    27961 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
    28021 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    28081 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    28141 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca cttcaaacac
    28201 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacagc
    28261 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    28321 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
    28381 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    28441 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    28501 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    28561 cagatgcgga tagcgttgct ggatatcagc cagcgagcgc cccatcaggc gtttaacaga
    28621 actaatggtg ttggcggtgt caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    28681 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    28741 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    28801 accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    28861 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    28921 cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
    28981 acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    29041 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    29101 cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
    29161 tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac ggatgacgca
    29221 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    29281 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    29341 tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagaaggtg aagtaatcca
    29401 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    29461 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    29521 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    29581 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    29641 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    29701 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    29761 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
    29821 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    29881 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    29941 cagcaatatc ggtatttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    30001 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    30061 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    30121 gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
    30181 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    30241 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    30301 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
    30361 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    30421 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    30481 tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc gactcacctt
    30541 caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca
    30601 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
    30661 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc attccgacga
    30721 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
    30781 gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata cctttcgggc
    30841 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    30901 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    30961 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    31021 aaacaaggat ggtgtcatca cgcatcgctg cttcaagttc tttcagatcg ataatgccgt
    31081 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    31141 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    31201 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    31261 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    31321 cttcagcctg ccagccgaaa cggtgagaac gggaggccgg gttaccaaag gttccgtcca
    31381 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    31441 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    31501 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
    31561 ttctaatatc ttgttatctt taacctaact gtttcgggtt tcagcaagtt gacaaactta
    31621 cgaatctccg ggagcttaca taagtaagtg accggagtga gtatgtgcag tcaacgcaga
    31681 tgcaacacga aacaggacgg ttaaattaag cgcgtaactt aacgtcgatc gcgtcttgta
    31741 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
    31801 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
    31861 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcaaccgcct ttaccctgac
    31921 aacgggtagc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
    31981 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
    32041 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
    32101 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
    32161 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
    32221 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
    32281 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
    32341 ccattttttt atcgtggcgt ttgtacctgt tgtccttcca gaccatgggc gggttgaggg
    32401 caattattcg cattagcgct ggcctcttcc cctcacccta accctctccc cgaaggggcg
    32461 aggggaccgt ccgtgcattt atctggctta gccctcaggc cttattacct ttattctgct
    32521 gctctataga agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac
    32581 gggtaaacag acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac
    32641 cggttgccag cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg
    32701 gatacggcgt ctcttcatgc tcgacctgct cgccattttc ctgagttgcc agccaggcca
    32761 tacgcacttc ataggcaata acctgaaccg ccattgccaa gttcagcgaa ctgtattccg
    32821 ggtttgccgc aatcgcgaca tgataatggc atttctgcaa ctcttcattg gtcaagccga
    32881 cgcgctcacg accaaacacc agcgccaccg gggtatttgc cgcttcagcg acgcttttca
    32941 gaccgcattc gcgcgggtcg agcatcggcc acggcagcgt gcgggagcgt gcgctggtgc
    33001 ccaccaccag gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac
    33061 caatcacatc gctggcccct gctgccaggg caatcgcctg ggagtcgggt ttcaccagtg
    33121 gattaaccag ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca
    33181 tattgccggt gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc
    33241 ttcatctaaa gattattcac gcatcttatc ataaaacgaa gacagatgcc gatctcgctg
    33301 ctatactctg cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga
    33361 tgctgaacat cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg
    33421 aaaccccgga cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg accaacgtag
    33481 ataaagctgc cgaagcggtg attatcgaca cgattcgtaa atcttactca cagcacacca
    33541 tcatcaccga agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg
    33601 atccactgga tggcactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg
    33661 ccgttcgtat caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac
    33721 tgttcaccgc cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggtagca
    33781 ccgctcgcga tctcgacggt accattctgg cgaccggctt cccgttcaaa gcaaaacagt
    33841 acgccactac ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc
    33901 gtaccggttc tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct
    33961 ttgaaatcgg tctgcgtccg tgggatttcg cggcaggcga gctgctggtt cgtgaagcag
    34021 gcggcatcgt cagcgacttc accggtggtc ataactacat gctgaccggt aacatcgttg
    34081 ctggtaaccc gcgcgttgtg aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg
    34141 ctctgaagcg ttaatgactc aggcgggtga tgtcactcac ccgccctcgc ctttcaggcg
    34201 ctattctgaa atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc
    34261 ccgattaatg gatgataaag aagtcacaag gaaaggttat ggcgctgcca gtgaacaaac
    34321 gcgttctcaa aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc
    34381 gcgttgccat caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt
    34441 cggcggaatc cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc
    34501 cttcttcgac gggccctgct gacaatgcca tcgcaaccat cattcatgct cacggcaatg
    34561 ccggaaatat gtccgcccac tggccgctgg tcagttggtt acccgagcgt aatttcaacg
    34621 tttttatgtt tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caggccggat
    34681 tgctggacga tacgcaaagt gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac
    34741 aacggctggt gctgttcggg cagagtattg gcggggcgaa tattcttgct gttattggtc
    34801 aggatgatcg tgaaggcata cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa
    34861 ccatcgccaa tcagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg
    34921 aaaattatat cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc
    34981 acgttatccc atggcagcac agcgaaaagt tgtatagcct ggcaaaagag acaaaacggt
    35041 tgatcctaat cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgatgttt
    35101 atcgcgaaca gatggtggac tttatcctta gcgcgttgaa tccgcagaac taacccatga
    35161 tcgctagcac gataatcatt cacaaaacca ccttaagaca tgctaatcca ctggtcagaa
    35221 cagtttaaga tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc
    35281 ttcgccatct ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta
    35341 cagatgatgc ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag
    35401 atcttgctga cgctctttca gccggggttg accgccacca tggcaacctt cagcgagctt
    35461 aatggtgtgg atgatgatat tgccagtctt gatatcagcg aaacaggacg ggagatcctg
    35521 cgctatcatc aactcacact gacgactggt tatgacggta gctaccgggt tgaaggtaca
    35581 gtgcttaacc aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtccg
    35641 tcatttatta ccagccaatt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt
    35701 gcgcgtaact tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacgg
    35761 ctgcaaaaac gctttgaaac gcgcgatatt catttcctgt gtctgtatct gcaatattgt
    35821 ttgctgcaac accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg
    35881 gaatcctgcc ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg
    35941 ctccagcctg taccgcctga tgagccactg tttatggcat tacttttttc catgttgcgg
    36001 gttcccgatc cattgcgtga tgcgcatcag cgagacagac aattgcgtca gtctatcaaa
    36061 cgtctggtaa accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta
    36121 tgcgatcagc tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt
    36181 attgataata ccctgccgga agagttcgcc agactgtacc cacgcctggt gcgcaccacc
    36241 cgcgcggcgc tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagc
    36301 ggtctggtcg cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa
    36361 cagattattc tactgaccgg gaatgatagc gagcgagaag cgcagattga gcagcagtta
    36421 cgcgaactaa cgttactgcc gctcaacatt aagcatatgt cggtaaaggc atttttgcag
    36481 acaggcgctc cgcgtggcgc ggcactgatt attgcgcctt ataccatgcc attaccgctc
    36541 ttttcaccac cgctgatcta tacggacctg acgctgacaa cacatcaaca ggagcagatc
    36601 cgcaaaatgc tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggaag
    36661 cgccaccagc gccattaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc
    36721 gaaaacggtc atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa
    36781 acggatgact tcgctaccct ggcgaacagc aatatagcgc atggcggcca ggtggcagac
    36841 cgtgaaggtg ccgcaatgca aaatttgcac cactatcaac cacggcaacg ccgtagttgc
    36901 tcccataatg ccccagcgca ctacgccgca aatcgccgag atcaacagca tatcgcgagc
    36961 actacaacgg cggaaaagtt tattactcag cgcaaagata atgacttccg ccaccacgcc
    37021 cagcgaccac aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc
    37081 gctaaaaccg taataggccg aatgtgcccc ctgcaataaa caaacgcagg ccagaaagcg
    37141 ccagttctgg cgaaccagcg ccaaccacgc agaccaaccg gtgctctcct gctggcgact
    37201 tgccccttgt ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc
    37261 caacgtcaac agcgcgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag
    37321 cgccgagcca atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg
    37381 gaactgcttt tgccacgtat tcgccagtgc atcggtcaac ggtaccagcg gtgagaaaaa
    37441 gaggttaaag ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc
    37501 gacagcaaag agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgggaagg
    37561 atcgctgacg cggggcgcga tgagcaaact cccgagaaaa cgggcaacca gacctgcccc
    37621 caataacagg ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa
    37681 aggtagaaaa atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt
    37741 ggattgcaaa accatgagtc cctcccgtca ggaggcgtta gtctggggtt aatcgaatgt
    37801 tggtgcaagt tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt
    37861 cacaagcact aaaaaactgc gatccgcgac gcatgttgca aaaataatgc gtaattatgc
    37921 cgttacgctt gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat
    37981 ctcccctttc ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg
    38041 aaaaacaacc tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag
    38101 ccccatgccc accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg
    38161 ctggctgttt tcttgcgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc
    38221 cggatcggta ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct ctttttcatg
    38281 tgctaaaggg tgatcaaccg gtaacaccac cactaatggt tcgtcaaaaa gctccagata
    38341 atcaatctcc gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg
    38401 aattttttcc tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg
    38461 tctgagacga aacattggta atacttttgg caataaattc acttccgccg atggcacaaa
    38521 gccaatggtt aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc
    38581 cgcttgttcg aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc
    38641 gactttgcgc ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat
    38701 ctggctgctt aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag
    38761 tgcctgcgcc actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt
    38821 atcgaaaaaa cgtctcagct ggtagaaatt aactatttca catattagca accaaccagc
    38881 aacatcctta tgacacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat
    38941 atgaccacac cctcagattt aaacatttac caactgattg atacccaaaa tggtcgggtc
    39001 actccgcgta tttataccga cccagatatt taccaactgg agcttgaacg tattttcggc
    39061 cgttgctggt tatttctcgc ccacgaaagc cagatcccaa aacctggtga tttctttaac
    39121 acctacatgg gggaagatgc ggttgtcgta gtgcgtcaga aagacggcag cattaaggcg
    39181 tttctcaacc aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact
    39241 cgcgccttta cctgtccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat
    39301 gtaccgctgg aacctcgcgc ctacccacaa gggttgtgta aatcccactg gggactaaac
    39361 gaagttcctt gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca
    39421 ccgggcctgc gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt
    39481 cgcgaaggcg gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg
    39541 aaattcccgg cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct
    39601 gccgttcagg tattaggggc gaaagatgat ggcagcgata agcgcctcgg tgatggacaa
    39661 accgcccgcc cggtgtggga aaccgccaaa gatgcgctgc aatttggtca ggacggtcac
    39721 ggtagcggtt tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt
    39781 tcaagctatt accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc
    39841 ctgcgcctgg cgggtcataa caatattttc cccacgcttt catggctcaa cggcactgcc
    39901 acgctccgcg tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt
    39961 actgacaaag ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct
    40021 tttggtcctg ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa
    40081 ttgcttaaag gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag
    40141 gaaaagcgcc gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc
    40201 gctcgtggaa tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg
    40261 ctcgataaaa ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag
    40321 agttacatca ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga
    40381 aatttcgtga ctggctggag cagctcgaca aagagattcg ttacaccatg cgcaccacag
    40441 ttaacgcgca aacacgcgac cgccgcaaag gcgtccagcc accgacaacc tggattttta
    40501 atgacaccaa agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcctggg
    40561 cagaagagcc gccgtcacgc acccgtcact taatcagcaa ctgccagata agcgaaaccg
    40621 acatcccaaa cgtatttgct gtgcgggtaa attatctgct ttatcgggca caaaaagagc
    40681 gcgatgaaac attctatgtt ggaacgcgtt tcgacaaagt tcgccgtctg gaagatgaca
    40741 actggcgctt gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc
    40801 tgagtgtact gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg
    40861 tgaggctctc cggatcgata cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt
    40921 ttatgccatt aacgatcgtt gcagccatgg taatgcgtcg atgtcagaag ggtatctgga
    40981 agatgacgcc acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa
    41041 agcgttatgc ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg
    41101 tgacattttc atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt
    41161 tttattaccg gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa
    41221 ggcgcgcaag ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga
    41281 tttggcgaac atattctggc ggtggaaggt aacgtgacct gttatgccga ttatcaacgc
    41341 gcggtcgatc agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcaggc
    41401 atctgggatc acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc
    41461 cacgagctgt ttaacgtcaa cgtactcggt tacctgctgg gcgcaaaagc ctgcgctccg
    41521 gcgttaatcg ccagtgaagg cagcatgatt ttcacactgt caaatgccgc caggtatcct
    41581 ggcggcggtg gcccgctgta caccgccagt aaacatgccg caaccggact tattcgccaa
    41641 ctggcttatg aactggcacc gaaagtgcgg gtgaatggcg tcggcccgtg tggtatggcc
    41701 agcgacctgc gcggcccaca ggcgctcggg caaagtgaaa cctcgataat gcagtctctg
    41761 acgccggaga aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggcggatttt
    41821 acggggccgt atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg
    41881 atcaacgctg atgcgggttt agcgattcgc ggcattcgcc acgtagcggc tgggctggat
    41941 ctttaaggaa gcacgatgaa agaaaaaacg atcattattg tcggtggcgg gcaagcggcg
    42001 gcaatggctg cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc
    42061 gatgagcgac atcttcctta tgaacgacct ccactctcga aatccatgtt gctggaagat
    42121 tccccgcagt tacagcaggt gttacccgct aactggtggc aggaaaacaa tgttcatctg
    42181 cattccggtg taaccatcaa aacgctgggt cgcgacacac gagagttagt gttaaccaac
    42241 ggcgaaagct ggcactggga tcagcttttt atagcaatcg gcgcggcagc tcgaccgctg
    42301 ccgttgcttg atgcactggg agaacgctgc tttaccctac gccatgccgg tgatgccgcc
    42361 agactgcgag aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt
    42421 ggtctggaac tggctgccag cgccacgcag cgcagatgta aggtgacagt gattgaactg
    42481 gcggcaaccg tcatgggccg taatgcacca ccgcccgtgc aacgctatct tttacagcgc
    42541 caccagcagg ctggtgtgcg cattctgctc aataatgcca ttgaacatgt ggtcgatggt
    42601 gaaaaagtag aactgacgct gcaaagtggg gaaacgcttc aggctgatgt ggtgatttac
    42661 ggtattggta tcagcgccaa cgagcaactg gctcgcgagg ccaaccttga tactgccaat
    42721 ggcattgtca ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat
    42781 gtggcaatca ctcgtcttga taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc
    42841 aataaccagg cgcaaattgc cgctgccgca atgttagggc taccgctacc gctactgccg
    42901 ccgccgtggt tctggagcga tcagtacagt gataacttac agtttattgg cgatatgcgt
    42961 ggcgatgact ggctttgtcg tggcaacccg gaaactcaga aggcgatttg gtttaatctg
    43021 caaaacggcg tgcttatcgg tgcggtcacg ctgaatcagg ggcgtgagat tcgcccaatt
    43081 cgcaaatgga tccagagcgg caaaacgttt gatgcgaaac tgctgataga tgagaacatc
    43141 gcgcttaaat cactgtaacc aggataatta gcgaatatct caacgcctgg ggcgtggcga
    43201 ggtgcaagag tgtgtattac gtttaaatca cattatcttg caaagggatt ggttatgaac
    43261 acacta
//
LOCUS       DAZPEP010000019        42133 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZPEP010000019 DAZPEP010000000
VERSION     DAZPEP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.019"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            448..600
                     /gene="mokC"
                     /locus_tag="ACHUT9_001013"
     CDS             448..600
                     /gene="mokC"
                     /locus_tag="ACHUT9_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFR1347552.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHUT9_001014"
     CDS             complement(704..1834)
                     /gene="dnaJ"
                     /locus_tag="ACHUT9_001014"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFR1347553.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHUT9_001015"
     CDS             complement(1923..3839)
                     /gene="dnaK"
                     /locus_tag="ACHUT9_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFR1347554.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4216..4620
                     /locus_tag="ACHUT9_001016"
     CDS             4216..4620
                     /locus_tag="ACHUT9_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFR1347555.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4646..5359
                     /gene="msyB"
                     /locus_tag="ACHUT9_001017"
     CDS             4646..5359
                     /gene="msyB"
                     /locus_tag="ACHUT9_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFR1347556.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            5508..6074
                     /gene="satP"
                     /locus_tag="ACHUT9_001018"
     CDS             5508..6074
                     /gene="satP"
                     /locus_tag="ACHUT9_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFR1347557.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHUT9_001019"
     CDS             complement(6109..6696)
                     /gene="mog"
                     /locus_tag="ACHUT9_001019"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFR1347558.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHUT9_001020"
     CDS             complement(6811..7764)
                     /gene="tal"
                     /locus_tag="ACHUT9_001020"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFR1347559.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            8043..9472
                     /locus_tag="ACHUT9_001021"
                     /pseudo
     CDS             8043..9472
                     /locus_tag="ACHUT9_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
     gene            9542..10318
                     /gene="yaaA"
                     /locus_tag="ACHUT9_001022"
     CDS             9542..10318
                     /gene="yaaA"
                     /locus_tag="ACHUT9_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFR1347560.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(10471..10767)
                     /locus_tag="ACHUT9_001023"
     CDS             complement(10471..10767)
                     /locus_tag="ACHUT9_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFR1347561.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="ACHUT9_001024"
     CDS             complement(10981..12267)
                     /gene="thrC"
                     /locus_tag="ACHUT9_001024"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFR1347562.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="ACHUT9_001025"
     CDS             complement(12268..13200)
                     /gene="thrB"
                     /locus_tag="ACHUT9_001025"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFR1347563.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="ACHUT9_001026"
     CDS             complement(13202..15664)
                     /gene="thrA"
                     /locus_tag="ACHUT9_001026"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFR1347564.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKTRDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15691..15810)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="ACHUT9_001027"
     CDS             complement(15746..15811)
                     /gene="thrL"
                     /locus_tag="ACHUT9_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFR1347565.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(16025..16711)
                     /locus_tag="ACHUT9_001028"
     CDS             complement(16025..16711)
                     /locus_tag="ACHUT9_001028"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1347566.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(17111..17251)
                     /gene="yjjY"
                     /locus_tag="ACHUT9_001029"
     CDS             complement(17111..17251)
                     /gene="yjjY"
                     /locus_tag="ACHUT9_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFR1347567.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17347..18063
                     /gene="arcA"
                     /locus_tag="ACHUT9_001030"
     CDS             17347..18063
                     /gene="arcA"
                     /locus_tag="ACHUT9_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFR1347568.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(18123..19475)
                     /gene="creD"
                     /locus_tag="ACHUT9_001031"
     CDS             complement(18123..19475)
                     /gene="creD"
                     /locus_tag="ACHUT9_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFR1347569.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(19533..20957)
                     /gene="creC"
                     /locus_tag="ACHUT9_001032"
     CDS             complement(19533..20957)
                     /gene="creC"
                     /locus_tag="ACHUT9_001032"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFR1347570.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20957..21646)
                     /gene="creB"
                     /locus_tag="ACHUT9_001033"
     CDS             complement(20957..21646)
                     /gene="creB"
                     /locus_tag="ACHUT9_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFR1347571.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21659..22132)
                     /gene="creA"
                     /locus_tag="ACHUT9_001034"
     CDS             complement(21659..22132)
                     /gene="creA"
                     /locus_tag="ACHUT9_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFR1347572.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            22343..23212
                     /gene="robA"
                     /locus_tag="ACHUT9_001035"
     CDS             22343..23212
                     /gene="robA"
                     /locus_tag="ACHUT9_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFR1347573.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="ACHUT9_001036"
     CDS             complement(23209..23856)
                     /gene="gpmB"
                     /locus_tag="ACHUT9_001036"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFR1347574.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23908..24429
                     /gene="yjjX"
                     /locus_tag="ACHUT9_001037"
     CDS             23908..24429
                     /gene="yjjX"
                     /locus_tag="ACHUT9_001037"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFR1347575.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="ACHUT9_001038"
     CDS             complement(24514..24840)
                     /gene="trpR"
                     /locus_tag="ACHUT9_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFR1347576.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="ACHUT9_001039"
     CDS             complement(24930..26867)
                     /gene="sltY"
                     /locus_tag="ACHUT9_001039"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFR1347577.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            27078..28745
                     /gene="ettA"
                     /locus_tag="ACHUT9_001040"
     CDS             27078..28745
                     /gene="ettA"
                     /locus_tag="ACHUT9_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFR1347578.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28906..29013)
                     /gene="nadS"
                     /locus_tag="ACHUT9_001041"
                     /pseudo
     CDS             complement(28906..29013)
                     /gene="nadS"
                     /locus_tag="ACHUT9_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
     gene            complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="ACHUT9_001042"
     CDS             complement(29052..30284)
                     /gene="nadR"
                     /locus_tag="ACHUT9_001042"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFR1347579.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30305..31687)
                     /gene="radA"
                     /locus_tag="ACHUT9_001043"
     CDS             complement(30305..31687)
                     /gene="radA"
                     /locus_tag="ACHUT9_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFR1347580.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31736..32704)
                     /gene="serB"
                     /locus_tag="ACHUT9_001044"
     CDS             complement(31736..32704)
                     /gene="serB"
                     /locus_tag="ACHUT9_001044"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFR1347581.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            32810..33454
                     /locus_tag="ACHUT9_001045"
     CDS             32810..33454
                     /locus_tag="ACHUT9_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFR1347582.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33482..34498
                     /gene="lplA"
                     /locus_tag="ACHUT9_001046"
     CDS             33482..34498
                     /gene="lplA"
                     /locus_tag="ACHUT9_001046"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFR1347583.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            34530..34793
                     /locus_tag="ACHUT9_001047"
                     /pseudo
     CDS             34530..34793
                     /locus_tag="ACHUT9_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="ACHUT9_001048"
     CDS             complement(34954..35673)
                     /gene="deoD"
                     /locus_tag="ACHUT9_001048"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFR1347584.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="ACHUT9_001049"
     CDS             complement(35753..36976)
                     /gene="deoB"
                     /locus_tag="ACHUT9_001049"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFR1347585.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="ACHUT9_001050"
     CDS             complement(37028..38350)
                     /gene="deoA"
                     /locus_tag="ACHUT9_001050"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFR1347586.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="ACHUT9_001051"
     CDS             complement(38477..39256)
                     /gene="deoC"
                     /locus_tag="ACHUT9_001051"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFR1347587.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39514..41064
                     /locus_tag="ACHUT9_001052"
     CDS             39514..41064
                     /locus_tag="ACHUT9_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFR1347588.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            41036..41899
                     /locus_tag="ACHUT9_001053"
     CDS             41036..41899
                     /locus_tag="ACHUT9_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFR1347589.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
BASE COUNT         9945 a        11408 c        10590 g        10190 t
ORIGIN      
        1 acacggaaag tcatatcagc gctaacgggt gaccctaagc cctgataatc atagcttaag
       61 gtcaggattt ctgagggatt aattaggaaa agtaataatt aatttcttgt cagtcggcgt
      121 tatttgttag gacaatattt caaaaataat ccagcttgtc gtcaataatt gcgataaata
      181 gataaattaa agttaaataa ttaaatcatt aaaaaattac gccacaaaaa tgcgaggcgt
      241 cttcaggatt agaatcgata aaacagaatg ggttaaagag agcgatataa tagcggcggg
      301 tgcttgaggc tatctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
      361 atattttact atcaacccga ggccccctat atgctgaaca catgtagagt gcctcttact
      421 gaccgtaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
      481 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
      541 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
      601 gagcaacggc ggggagtgat ccccgccact tttcaggtgc tcgcatatct tcaacgcacc
      661 ctaattttac ccaagcctgc ccacgggcag gcttttgggg aggttagcgg gtcaggtcgt
      721 caaaaaactt cttcacacca tcaaagaagc tctttgagcg cgggctgttg tgctcaccgg
      781 ttgggccacc gaagctttct tgcagctctt gcagcaactg tttctgcttc tcgttcaggc
      841 ctaccggtgt ttcgacgaca acgcggcaca gcaaatcacc ctgtgcgcca ccgcggacag
      901 acttgacgcc tttaccgcgc atacggaaaa gcttaccggt ctgggtttcg ccaggcactt
      961 tcagtttgac gcgaccatca agggtcggta cttcgatttc gccacccagc gccgccatag
     1021 cgaagttgat cgggacttcg caatagaggt tgttgccttc acgctcgaaa atcgggtgct
     1081 gtttaacctg aacctgaacg tacagatcgc ctgccggtgc gccatgctcg cccgcttcac
     1141 cttcgcccgc aagacggatg cggtctccag tgtccacccc tgccgggatt ttaacggaca
     1201 gcgttttgct gcgctcaaca cgaccatgac catgacattt gttgcacgga tctttgatca
     1261 gcgtaccgcg gccctgacag tgtggacagg tctgctgtac agcgaagaat ccctggcgca
     1321 tctgcacctg accagaacca tgacaggtcg gacaggtctg cggctgtgta cctggttttg
     1381 caccgctacc gtggcaaacg tcacactctt ccagagtcgg aatgcggatc tctttggtca
     1441 cgccacgtac agcttcttcg agggtgagct ccatgttata gcgtaaatca gcaccgcgcg
     1501 ccgcacgttg acgaccacgt ccgccgccaa aaatatcgcc gaaaacgtca ccaaaaatat
     1561 cgctgaagtc tgcgccgccg ccaaaaccgc cgccgcccat gccaccttgc tcaaacgcag
     1621 catgaccata ctgatcgtat gccgcacgtt tttgcgagtc ggtcagaact tcataagctt
     1681 ccttgatctc tttaaatttc gcctcggcct ctttgtcacc ctggttacgg tccgggtggt
     1741 atttcatggc caggcgtttg taggcctttt tgatttcacg ctcttccgct gttttggaaa
     1801 cgcctaaaat ctcgtaataa tcttgcttag ccatcttttt taaattgccc ctagatgaat
     1861 gcacgggcgg agaggaaatt ccccttcgcc cgtgtcagta taattacccg tttatagggc
     1921 gattattttt tgtctttgac ttcttcaaat tcagcgtcga caacatcgtc atctttcgcg
     1981 ttgtttgcag aagcatcagc accggcagtc tgctgctggg catgttgctg ctgggcgatt
     2041 tccatcagtt tctgggaaac ctgtgccagt tcctgcattt tcgcttcgat agcggctttg
     2101 tcttcacctt tcagagcagt ttccagtgca gtcagcgcag actcgatagc agttttgtcg
     2161 tcagccggca gtttgtcgcc tgcttcttca acctgcttac gggtgctgtg cagcagatgg
     2221 tcgccctggt tgcgagtctg taccagctct tcaaacttac ggtcagcttc ggcgttagct
     2281 tctgcgtcgc gtaccatttt ctggatttca tcttcgttca gaccagaaga agccttgatg
     2341 gtgatcttct gctctttacc gctgttttta tctttcgcgg aaacgtgcaa gataccgtca
     2401 gcatcgatat cgaaggtaac ttcgatctgc ggcatgccgc gcggtgccgg gttgatacca
     2461 tccaggttga actgacccag agatttgtta tcagctgcac gtttacgttc accctgcagc
     2521 acatggatgg ttaccgcaga ctggttgtct tcagcggtag agaacacctg gctgtgcttg
     2581 gtcgggatag tggtattttt cgcgatcagc gtcgtcatca caccgcccat ggtttcgata
     2641 cccagagaca gcggggtaac gtccagcagc agtacgtctt ttacgtcacc agtcagaaca
     2701 ccaccctgaa cagcagcacc gattgctaca gcttcgtccg ggttaacgtc tttacgcggc
     2761 tctttaccaa agaactcagc aactttcttc tgaaccattg gcatacgagt ctgaccaccc
     2821 acgaggataa cgtcgtcgat atcagatacg gacaggccag cgtcctgcag tgcaactttc
     2881 agcggctcaa tggaacggtt taccagatct tcaaccaggc tttccagttt cgcacgagtc
     2941 actttgatgt tcatgtgttt cggaccggtc gcatctgcag tgatgtacgg caggttaacg
     3001 tcggtctgct gagcggaaga cagttcgatt ttcgcttttt ctgccgcttc tttcaggcgc
     3061 tgcattgcca gcggatcgtt gcgcaggtca atgccctgat ctttcttgaa ttcttcaacc
     3121 aggtagttga tcagacggct gtcgaagtct tcacccccca ggtgggtatc accgttggta
     3181 gccagaactt cgaaggtttt ttcgccgtca acttcgtcga tttcgataat agaaatatcg
     3241 aaagtaccac cacccaggtc ataaaccgcg atagtacggt tgccagtacc tttgtccaga
     3301 ccgtaagcca gcgcagctgc ggtcggttcg ttgatgatac gttttacttc cagaccagcg
     3361 atacggcctg cgtctttggt tgcctgacgc tgagcatcgt taaagtatgc cggtacggtg
     3421 ataacagctt cagttaccgg ttcacccaag taatcttcag cggttttctt catttttttc
     3481 agcacttcag cagaaatctg cggcggtgcc attttctggc ctttaacttc gacccatgcg
     3541 tcgccgttat cagcagcaat aattttgaac ggcatgatgg aaacatcacg ctgtacttct
     3601 tcgtcctgga agcggcggcc aatcaggcgt ttaatcgcaa acagggtgtt ttgcgggttc
     3661 gtcactgcct gacgtttagc cggctgacca accagagttt caccatcctg ggtataggca
     3721 ataatagaag gcgtggtgcg atcgccttcg gcgttctcca gcacgcgagg agtggtgcca
     3781 tccataatcg ctacacaaga gttggtagta cccaggtcga taccaattat tttacccatc
     3841 taaacgtctc cactatatat tcggtcatca tgtggttgtg agtctgtaat aggggcgaaa
     3901 cgtctggttt caactgccca atttcatttt tttgcagact cactcactgc ggttgactac
     3961 taaatggggt cgtaacccac gtcatcaagg gggagatgca aaaatttttt gtgcttttgc
     4021 gcagaaattt cgcgatatgg caggacaaga cgtaaaattg acagcaattt tattcatata
     4081 aagaatgaat cgcagccaag gcattcatca aaaaattgta ataaaaagaa aagattacgt
     4141 acctgaatct tctctttatc agcagtaaac tagtgggtat tcatccctct acctcttccc
     4201 actaagagaa tccttatgaa atccgttttt acgatttccg ccagcctggc gattagcctg
     4261 atgctgtgct gcacggcgca ggcaaacgac cataaactcc tcggcgtcat tgcaatgccg
     4321 cgtaacgaaa ccaacgatct ggcgctgaaa cttcctgttt gtcgcattgt gaaacgcata
     4381 caactctctg ccgaccatgg cgatttacag ttaagcggtg catcggttta tttcaaagcc
     4441 gcccgtagcg ccagtcagag tctgaatatt ccttcagaaa taaaagaagg gcaaaccact
     4501 gactggatca acattaacag tgataacgac aataaacgct gcgtctcaaa aatcaccttt
     4561 tccggtcata cggtgaactc atcggatatg gccacgctga aaattatcgg cgacgactaa
     4621 cgccagaata tagaaaccac aaaaaatgaa tgttaattac ctgaatgatt cagatctgga
     4681 ttttcttcag cattgtagtg aggaacagtt ggcaaatttc gcccgattgc tcacccataa
     4741 tgaaaaaggc aaaactcgcc tctccagcgt actgatgcgc aacgaactgt ttaaatcgat
     4801 ggaagggcat cccgagcaac atcgccgcaa ctggcagctg attgctggag aattacagca
     4861 ttttggtggc gatagtatcg ccaacaaact gcgcggacac ggtaaattgt atcgggccat
     4921 tttgctcgat gtttcaaaac gattgaagct gaaagccgac aaagagatgt ctacgtttga
     4981 gattgagcaa cagttactgg aacaatttct gcgtaacacc tggaagaata tggacgagga
     5041 acataagcag gagtttctgc acgcggtcga tgccagggtg aatgagctgg aagagctgct
     5101 gccgctgctg atgaaagaca aattattggc aaaaggcgtg tcgcatttgc tttccagcca
     5161 attgacccgc attttacgca cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg
     5221 cggcgcgggt ctgggaggcc ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg
     5281 cagtgcctat cgcgtgacaa ttccagccgt aatacaaatc gcctgcctgc gccggatggt
     5341 tagcgccact caggtctgac accgtaaacc ggggaaaatt ttcattttcc cctcaaaaga
     5401 tcgtagacac tgccccactg gctgattatt atgccgcgcc ctgaaaacac tactgttatt
     5461 cagggaaatt atttcaccat tcattcgatg atgatttttg aggaattatg ggcaacacta
     5521 agttggctaa tccggcaccg ctgggcctga tgggcttcgg catgaccacc attctgctta
     5581 acctgcacaa cgtgggttat ttcgctctgg acggtattat tcttgccatg ggcattttct
     5641 acggcggcat cgcgcaaatt tttgccggtc tgctggagta caaaaaaggc aacactttcg
     5701 gtttaaccgc attcacctct tacggttctt tctggttgac gctggttgcg attctgctga
     5761 tgccgaaact gggtctgacc gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc
     5821 tgtggggcgt atttacgctg tttatgttct tcggcacgct gaaaggcgca cgcgttctgc
     5881 aattcgtttt ctttagcctg actgtgctgt ttgccctgct ggcgatcggt aacattgccg
     5941 gtaacgccgc aatcatccac tttgccggct ggattggtct gatctgtggt gccagtgcaa
     6001 tctatctggc gatgggtgaa gtactgaacg agcagtttgg tcgcaccgtt ctgccgattg
     6061 gtgaatccca ctaattgttt tgagatcccc ccgctcgggg ggattttttt attcgctaac
     6121 gtcgcgtctt gcactcttcg gtctgaatgc agcaaccact tctggtgccg tttcaacgta
     6181 tggcccttcc agcaactgaa tgcagtacgg tacgctggca aaaataccgt gtaccacaac
     6241 gttacccgca gcgtccttca caccttccag cgtctcttta atagacttcg gctgaccggg
     6301 taagttaagg atcagcgcct gtttgcgaat cacaccaacc tgacgcgaaa ggatcgcagt
     6361 tggtacaaaa tgcaggctta tctggcgcat ctgttcacca aagccaggca tctcgcggtc
     6421 cgctactgcc agcgtcgcat cgggcgttac gtcacgacgc gccgggccag ttccgcccgt
     6481 ggtgagcacc agatggcaac tcatttcatc cactagctca cacaacgttt gctcgatgat
     6541 cgcctgctca tcggggatta agcgggtttc cagttcaaac ggcgtggtta gcgccgatgt
     6601 cagccattct tccagcgcag ggatgccttt atcctgataa acgccgctgg atgcgcgatc
     6661 agagatggaa actaagccaa tacgtaaagt attcatcaag atttttccgt ttaaacagtc
     6721 tcgttaaaca gaatgataca ctgcgaaggg agtgacagac aggcttcaga agaggtagcg
     6781 tgaccgactt cccggtcacg ctaagaatga ttacagcaga tcgccgatca ttttttccag
     6841 tttttcctgg tcaatagcaa acttacggat accttccgcc agtttatcta ctgccattgg
     6901 atcctggttg tgctgccaca ggaactcgga ctcagtgata cgcgccggac gcgctttcac
     6961 ttcgccggtg taagacagtt tacgttcgat agccccttcg ctctcagcca gctctttcag
     7021 cagtgccggt gcgatggtaa gacggtcgca gcctgccagt tccagaattt cgccgatgtt
     7081 acggaagctt gcgcccataa ccacggtttc ataaccgtgc tctttgtagt actggtagat
     7141 ttcagataca gaaaccacgc ccggatcttc tgccggagcg tactctttct tatcagtatt
     7201 cgctttgtac cagtcaagaa tacggccaac aaatggcgag atcaggaaca cgcctgcttc
     7261 cgcacaagca cgagcctgag cgaaggagaa cagcagggtc aggttacagt tgatgccttc
     7321 tttttccagc tgttctgcag cacggatacc ctgccaggta gaagccagtt tgatcagaat
     7381 acgatcgttg ctaataccag catcgttgta gagtttgatc aggcgttttg ctttcgcaat
     7441 tgacgcttcg gtgtcatagg aaagacgcgc atcaacttca gttgagatac ggcccggaac
     7501 cagtttcagg atttccagac caatatttac tgccagtttg tcggtcgcgt ccacgatctg
     7561 ctgcgcgcga tcgttgctct gctgtttcgc ccaggcgaca gcatcatcaa tcaacttacg
     7621 gtattccgga atctgcgctg cgttaagaat gagagaaggg ttggttgtgg catcctgcgg
     7681 ttgatacagc ttcattgccg cgatgtcccc agtgtcggcc actacggtgg tgtactgacg
     7741 aagggaggtc aatttgtccg tcatgatagt atttctcttt aaacagcttg ttagggggat
     7801 gtaaccggtc tgccctgatg atatcacgac gcaacgtcag cgcaaccgcc gacaagacgg
     7861 ttatcgccag gcgagactgt ttcggatttc tgacactgcg ttgatatcgc ccgccatttt
     7921 tatacaaaac ctcatgtatg ctacgcagaa gttatcaagt acctcgtagc gtatatactt
     7981 cttaaacaat tggtaacgtt tacacaggaa agtcatcgcg accggcaata agagggatat
     8041 gcatgccaga ttttttctcc tttattaaca gcgtcctttg gggatcggta atgatttacc
     8101 tgctcttcgg cgcaggttgt tggttcactt ttcgcaccgg atttgtgcag tttcgctaca
     8161 tccgccagtt tggcaaaagt cttaaaaata gcattcatcc acagccaggc ggtttaacct
     8221 catttcagtc attgtgtacc agtcttgcgg cgcgcgtggg tagcggcaac ctggccggcg
     8281 ttgcgctggc tattaccgcc ggtggacctg gagccgtctt ttggatgtgg gttgccgcgt
     8341 ttatcggcat ggcgacctcg tttgccgaat gttcccttgc acaactttat aaagaacgtg
     8401 acgtcaatgg acagtttcgt ggaggaccgg catggtatat ggcgcgcggg ctggggatgc
     8461 gttggatggg cgttctgttc gccgtctttt tgctcatcgc ctacggcata attttcagcg
     8521 gagttcaggc gaacgccgtt gcccgagccc tgagttttct tttgattttc ccccgctggt
     8581 gacaggcatt attctcgctg tctttgctct gctggcaatc actcgcggtc ttcatggcgt
     8641 cgcccggctc atgcaggggt ttgtcccgtt gatggcgata atctgggtgc tgaccagcat
     8701 ggtgatttgc gtaataaata tcgggcaact tccccacgtc atttggtcta tttttgagag
     8761 tgcttttggc tggcaggaag cggcaggcgg cgcggcggga tataccttaa gccaggcgat
     8821 tactaacggt tttcagcgca gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa
     8881 cgcggcagcg gcagcggcgt cctggcctcc gcatccggca gcgcaaggga ttgtccagat
     8941 gattggcatt tttatcgaca ccctggtcat ctgtacggca agcgccatgc tgatattact
     9001 ggcgggtaac gacacaacct acatgccgct ggaaggtatt cagcttatcc agaaggcgat
     9061 gcgggtgcta atgggttcct ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc
     9121 cttcagctcc atcgttgcca actacattta tgccgaaaac aatctcttct ttttacgcct
     9181 gaacaaccct aaagcgatct ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg
     9241 caccttgcta agtcttccgc tgatgtggca actggcagat atcataatgg cctgcatggc
     9301 tattaccaat ttgaccgcca ttttactgct ctcgcctgtg gttcatacca ttgccagtga
     9361 ttatctacgc cagcgtaaac tcggcgtgcg cccggtgttt gatccgttgc gttatccgga
     9421 tatcggtcgc cagctttctc cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact
     9481 atcgatcaac tcattctctt tttttgctaa agtcggcata aatttcctgc aaggactgga
     9541 tatgctgatt cttatttcac ctgcgaaaac gcttgattac caaagcccgt tgaccaccac
     9601 gcgctatacg ctgccggagc tgttagacaa ttcccagcac ttgatccatg aagcgcggaa
     9661 actgacgcct ccgcagatta gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa
     9721 cgccgctcgc tttcatgact ggcagccaga tttcacgccg gagaatgccc gccaggctat
     9781 tttggcgttt aaaggtgatg tctacaccgg cttgcaggcc gaaactttca gcgaagacga
     9841 tttcgatttt gcccaacagc atttgcgaat gctttccggc ttgtatggcg tactgcgccc
     9901 gctcgattta atgcagcctt atcgtctgga aatggggatc cgtcttgaga atgcccgagg
     9961 gaaagatctg tatcaattct ggggagatat catcaccaac aagctgaacg aggcgctcgc
    10021 agcacaaggc gataatgtgg tgattaatct ggcgtcagat gagtatttta aatcggtgaa
    10081 gccgaagaaa ttgaatgccg agattatcaa gccggtgttc ctcgatgaga agaacggcaa
    10141 atttaagata atcagcttct acgctaagaa agcacgcggt ctgatgagtc gtttcattat
    10201 tgaaaatcgg ctgaccaaac cagagcaact gactggtttt aatagcgaag gttacttctt
    10261 tgatgaagct tcctccagca atggcgaact ggtgtttaaa cgctacgagc agcgttaagt
    10321 ttgcagtcaa tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag catgcagatt
    10381 caatatattg cagaattcac gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg
    10441 aatgccggat tgttacccgg catttgtcat ttagcggtga tgtttgcctg gaccatgacc
    10501 gccgtgatga tcatgaggag ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg
    10561 gtgccagcga ttgcctcgcc attcataatg ttgtttccac cagccgtggt cgcgccagtg
    10621 acctccatcc cagtaatagc cacgattatc acgatcgcct atctgtaatt ttactgacgg
    10681 gactaacgta atttccgcag cctgtgctgc catgggagcg accagaacca gggaaagtgc
    10741 gagtacgata gattgcatct ttttcacagg ttactccttt tatcattgcc cgttatgggt
    10801 cgatatccgg agattacgcg atgagaacga cggttgttat tctccgcaat cctaatctct
    10861 aagttaccgc atttattgag aatttctcct ttttccctgt cgtttttctc cataatttct
    10921 tcataaaaaa gccgggctgc ataaaagcaa acccggcctg attgagataa tgaatagatt
    10981 ttactgatga ttcatcatca atttacgcaa cgcagcaaaa tcggcgggca gattatgtga
    11041 aagcaagggt aaatcagcac gttctgccag ctcttttggc agatccaacg tttcaccgag
    11101 aatcgcttcc acgctctctt taaatttcgc cggatgcgcg gtgccgagga acaagccata
    11161 ttcgcctgga ttcaactggt cacacagcgc acgatacgct acggcagcgt gcggctccga
    11221 ggtgtagccc agttctttta actcacgcat tgtctgttgc gtggtttcat catccacggc
    11281 tgcataaccc agctctttca gttgccagat tttgcggcgg aacaactctt ccacacgcgg
    11341 ccagttgttc ggctgactca cgtccatcgc gttggataac gtcgcctgag tcgctttggg
    11401 tgaccactga ccgtcgtgca ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat
    11461 aaaacgtttc accggcagac cgagtgactt cgccagcaga cccgccgtca aatcgccgaa
    11521 gtttccgctt ggcaccgaga caaccagctg gttgcgcgct tcctgcggca gctgcgcaac
    11581 tgcttcaaag tagtagcaaa tctgcgccag caaacggctg atgttaatcg agttagccga
    11641 gtttaacccc agcgccactt tcagttcttc atcatcaaac gcctgcttca ccagcgcctg
    11701 acaggcatcg aaatcgccgt cgatggcaac agtttcgata ttgccgccca acgtacagaa
    11761 cagtttttct tgcagtggac tgattttgcc tcgtggatag aggataacca ctttcacatt
    11821 cggcaaaccg tagaaagcat gagccactgc cgctccggta tcaccggagg tcgcggtcag
    11881 aatggtcact ggcttatcgc ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc
    11941 gaaatcttta aatgccagcg ttggcccgtg gaacaattcc agacaaccga catcgctttc
    12001 aacattggcg accggagccg ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc
    12061 ctgcgggatt tcatcaccaa taaacgccga gaggatcttc gcactgcggg tgacaaaatc
    12121 cagcttcagc atctcatcaa tttcagtcag gctgaattcc ggcaggtcat gcggaaaaaa
    12181 cagcccctga tttttgccca acccctgggt tacggcttgc gcaaagctga cctgctcgtt
    12241 gtgatctttc agattgtaga gtttcattta gttttccagt actcgtgcgc ccgccgtatc
    12301 cagccggcaa atatgaacaa aaccttcctg attttgcagg tagttcttac ccaaccagtc
    12361 ggcaacgcgc tgggcggtat ccggcttgtc acatagagcg aacaaggtcg ggccggagcc
    12421 ggagataccg ctcgctaccg cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc
    12481 aggcagtaac cgttcacggt agggttcagc gataacatct ttcatcagct tcgcggcaag
    12541 ctcaggctga cgggaatagc aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat
    12601 gcaatcctgg cggcgatact gcgccggtaa aatagcccgg gcttctgccg tcgagacttt
    12661 aattcccgga tacgccagca cccacagcca ctcatcaaac cctggcactt gctggctgat
    12721 gatgtcgttt tcttcgatca tcaactgcat accaccaaga aaacacggtg ccacgttgtc
    12781 gtaatgaatg ctgccggaga tacgtccttc cagctcgccc atcaaagcca gcaaacgggt
    12841 gtcattaagt ggcttgccgc agtgttcatt catcgccatc agcgccgcga ccaccgaaca
    12901 ggcgctggag cctaagcccg agccgatcgg catattcttt tccagagtca tcgccactgg
    12961 aatttgcttg cccagctcca ggcaaaaacg ctcccagcac tgataaacga tattttcccg
    13021 tggttctgac ggcagcttat cggcaaagcg tccgaggttg ttgagactga atgtctctgc
    13081 cgcctcaacc gtgactacat ctccgagcaa tgcaccatca acgggtgtca ccgccgcccc
    13141 gagcacatca aacccgacgc tcatattggc actggaagcc ggggcataaa ctttaaccat
    13201 gtcagactcc taacttccat gagagggtac gtagcagatc ggcaaagaca ccagcagcgg
    13261 taacgtcatt gcccgcacca tatccgcgca gcaccaacgg cagcggctga taatagtggc
    13321 tataaaaggc cagggcgttt tcgccatttt tcactttgaa cagcggatca ttcccatcca
    13381 cttcggcaat cttcacgcgg cagacaccat cttcatcaat attgccaaca tagcgcaaaa
    13441 cttttccttc atcacgggtc ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca
    13501 gattcgccat aaaagcggca acatcaccct cggcgttaaa ctctgcgggc agcacaggtt
    13561 caatttcaat atccgccagc tccagttcac gtcccgtttc acgagcgaga atcaatagtt
    13621 tacgcgccac atccatacca gaaagatcat ctcgcgggtc cggttcggta taacccattt
    13681 cccgcgccag cgtggtcgcc tcggagaaac tcatgccttc gtctaacttg ccgaagatat
    13741 aagaaagcga acctgaaaga atgccggaga acttcaccaa ttcatcacct gcattgagca
    13801 gattttgcag gttctcaata accggtaatc cagccccaac gttggtgtca tagaggaatt
    13861 tacgccgcga tttttccgcc gcataacgca actgatgata gtaatccatc gacgaggtgt
    13921 tggccttttt gttcggcgtg acaacgtgga aaccttcgcg caggaagtcg gcatattgat
    13981 ccgccactgc ctggctggag gtgcagtcaa caatgaccgg gttcagcaga tgatattctt
    14041 tcacgaggcg aattaagcgc ccgagattaa acggctcttt ggcttgtgca agttcttcct
    14101 gccagttttc cagattaagg ccatgcacac tagtgagcag agccttcgag ttggcaacgc
    14161 cgcagacacg taagtcgata tgtttattct tcagccagct ttgctgacgc ttcagttgct
    14221 ccagcagcgc accgccaacg ccaccgacgc caatcacaaa cacttcgata acctgatcgg
    14281 tattgaacag catctgatga gtaacgcgca cgccagtggt cgcatcatcg ttatttacca
    14341 cgacagagat tgagcgttca gaagatccct gagcaatggc gacaatgttg atattggcgc
    14401 gggccagcgc ggcaaagaat ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta
    14461 ccaccgagat aatggccagc cgttccatca ccgccagcgg ctccagtaag ccttctttca
    14521 gttccaggta gaactcttcc tgcattgccc gttcagcccg cacacagtcg ctttgcggaa
    14581 cgcagaaact gatactgtat tcggaagatg attgcgtaat cagcaccacg gaaatacggg
    14641 cgcgtgacat cgcggcaaag acgcgcgccg ccatgccgac cattcctttc atccccgggc
    14701 cggaaacgct gaacattgcc atgttattca gattggaaat gcccttgacc ggtaattcgc
    14761 cttcatcacg actggcacca atgagcgtac ctggtgcttg aggatttccg gtatttttaa
    14821 tcaggcaagg gatctggaac tgggcgatgg gggtaatggt gcgggggtga agaactttag
    14881 cgccgaagta ggaaagctcc atcgcttcct ggtaggacat cgacttcaac aacctcgcat
    14941 cgggcacctg acgcgggtcg caggtataga ccccgtcaac gtccgtccaa atctcgcaac
    15001 aatcggcgcg taaacaggca gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa
    15061 gtaccaccag ttcgcctttt tcattaccgg cggtgaagcc tgccatcagc accatgtgat
    15121 cagccggaat acgacttgcc gcaatacggc gggtggactc agcaatatcg acggtagatt
    15181 cgaggtaatg ccccactgcc agtagttttt cgaccggatc gataacggta acgttgtgac
    15241 cacgcgcttc taacacgccg gccataatgg cgatcgacat tttctcgcca cggcaaatca
    15301 gcgcagcgtt aatgctatcc gggcactgcc ctaacaaact aatgccatgc aggacatgtt
    15361 ttatttgggc aaattcctgg tcgacgaaag ttttcaattg cgccagcggg aatcccggct
    15421 gggcggcggc gagtcccgtc aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg
    15481 gtaaagcatc ctggccgcta atggtttttt caatcatcgc caccaggtgg ttggtgattt
    15541 tggcgggggc agagaggacg gtggccacct gcccctgcct ggcattgctt tccagaatat
    15601 cggcaacccg cagaaaacgt tctgcatttg ccactgatgt accgccgaac ttcaacactc
    15661 gcatggttgt tacctcgtta cctttggtcg aaaaaaaaag cccgcactgt caggtgcggg
    15721 cttttttctg tgtttcctgt acgcgtcagc ccgcaccgtt acctgtggta atggtgatgg
    15781 tggtggtaat ggtggtgcta atgcgtttca tggatgttgt gtactctgta atttttatct
    15841 gtctgtgcgc tatgcctata ttggttaaag tatttagtga cctaagtcaa taaaatttta
    15901 atttactcat ggcaggtaac cagttcagaa gctgctatca gacactcttt ttttaatcca
    15961 cacagagaca tattgcccgt tgcagtcaga atgaaaagct gaaaaatact tactaaggcg
    16021 ttttttattt ggtgatattt ttttcaatat catgcagcaa acggtgcaac attgccgtgt
    16081 ctcgttgctc taaaagcccc aggcgttgct gtaaccagtc gaccagtttt atgtcatctg
    16141 ccactgccag agtcgtcagc aatgccatga ctcgttcgcg taaagcttgc agttgatgtt
    16201 ggtctgccgt tgtgtcactt ttcgccggtt gttgtattaa tgttgctaat tgatagcaat
    16261 agaccatcac cgcctgcccc agattgagcg aaggataatc cgccaccatc ggcacaccag
    16321 taagaacgtc agccaacgct aactcttcgt tagtcagccc ggaatcttcg cgaccaaaca
    16381 ccagcgcggc atggctcatc catgaagatt tttcctctaa cagcggcacc agttctactg
    16441 gcgtggcgta gtaatgatat ttcgcccgac tgcgcgcagt ggtggcgaca gtgaaatcga
    16501 catcgtgtaa cgattcagcc agtgttggga aaactttaat attatcaata acatcaccag
    16561 atccatgtgc gacccagcgg gtggctggct ccaggtgtgc ctgactatcg acaatccgca
    16621 gttcgctaaa acccatcgtt ttcattgccc gcgccgctgc cccaatattt tctgctctgg
    16681 cgggtgcgac caggataatc gttatacgca tattgccact cttcttgacc aaataaccgc
    16741 gaacagggtg atcactgtca acttattacg cggtgcgaat ttacaaattc ttaacgtaag
    16801 tcgcagaaaa agccctttac ttagcttaaa aaaggctaaa ctatttcctg actgtactaa
    16861 cggtttaatt gttaaaaaat gctacatatc cttctgttta cttaggataa ttttataaaa
    16921 aataaatctc gacaattgga ttcaccacgt ttattagttg tatgatgcaa ctagttggat
    16981 tattaaaata atgtgacgaa agctagcatt tagatacgat gatttcatca aactgttaac
    17041 gtgctacaat tgaacttgat atatgtcaac gaagcgtagt tttattgggt gtccggcccc
    17101 tcttagcctg ttatgttgct gttaaaatgg ttaggatgac agccgttttt gacactgtcg
    17161 ggtcctgagg gaaagtaccc acgaccaagc taatgatgtt gttgacgttg atggaaagtg
    17221 catcaagaac gcaattacgt actttagtca tgttacgccg atcatgttaa tttgcagcat
    17281 gcatcaggca ggtcagggac ttttgtactt cctgtttcga tttagttggc aatttaggta
    17341 gcaaacatgc agaccccgca cattcttatc gttgaagacg agttggtaac acgcaacacg
    17401 ttgaaaagta ttttcgaagc ggaaggctat gatgttttcg aagcgacaga tggcgcggaa
    17461 atgcatcaga tcctctctga atatgacatc aacctggtga tcatggatat caatctgccg
    17521 ggtaagaacg gtcttctgtt agcgcgtgaa ctgcgcgagc aggcgaatgt tgcgttgatg
    17581 ttcctgactg gccgtgacaa cgaagtcgat aaaattctcg gcctcgaaat cggtgcagat
    17641 gactacatca ccaaaccgtt caacccgcgt gaactgacga ttcgtgcacg caacctgctg
    17701 tcccgtacca tgaatctggg tactgtcagc gaagaacgtc gtagcgttga aagctacaag
    17761 ttcaatggtt gggaactgga catcaacagc cgttcgttga tcggccctga tggcgagcag
    17821 tacaagctgc cgcgcagcga gttccgcgcc atgcttcact tctgtgaaaa cccaggcaaa
    17881 attcagtccc gtgctgaact gctgaagaaa atgaccggcc gtgagctgaa accacacgac
    17941 cgtactgtag acgtgacgat ccgccgtatt cgtaaacatt tcgaatctac gccggatacg
    18001 ccggaaatca tcgccaccat ccacggtgaa ggttatcgct tctgtggtga tctggaagat
    18061 taatcggctt taccaccgtc aaaaaaaacg gcgcttttta gcgccgtttt tatttttcaa
    18121 ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt ctttactggc tttcattttc
    18181 ggcagtgaaa acgcatacca gtcgatatta cgagtcacaa acatcatgcc ggccagcgcc
    18241 accaccagca cactggttcc caacaacagc gcgctatcga cagagttgag cagtccccac
    18301 atcactccat ccagcaacaa cagcgcgagg gtaaacaaca tgctattgcg ccagcctttc
    18361 aataccgcct gcaaataaat accgttcatt aacgccccaa tcagactggc gattatccat
    18421 gccacggtaa aaccgatatg ttcagaaagc gccagcaaga gcaaataaaa catcaccaat
    18481 gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt gcgcggtgag cgtttcaaaa
    18541 acaaagaacg ccataaaagt cagtgcaatc agcagaatgg cgtacttagt cgcccggtcc
    18601 gttaattggt attgatcggc tggcgtcgtc actgcgacgc taaacgcgag gaagttttcc
    18661 cagccggtat cattgcctga agcaaaacgc tcaccgagat tattagcaaa ccagctgctt
    18721 tgccattgcg cctgaaaacc tgactcgcta acttcccgtt tggctggcaa aaaatcacct
    18781 aaaaaactgg gatgcggcca gttgctggtt aaggtcattt cgctattacg cccggcaggc
    18841 accacagaaa gatcgccggt accgcttaaa ttcagggcca tattcagctt caggttctgc
    18901 ttccgccagt ccccttcggg taaagggata tgcacgccct gcccgccttg ctctaacccg
    18961 gtgccgggtt caatggtcag cgccgttccg ttaacttcag gcgctttcac cacaccaata
    19021 ccacgcgcat ccccgacgct aatcacaata aatggcttgc caaaggtgat atttggcgcg
    19081 tcgagttcgc taagacgcga aacatcgaaa tcggctttta gcgttaaatc actgtgccag
    19141 acctgaccgg tataaatccc tatcttgcgt tcttccacgt tctgattgcc atcaaccatc
    19201 aatgactcag gtaaccaaaa atggataaaa cttcgtttcc gctccacggt tttatcctct
    19261 tcctgtaccg tataaagctc ggtcacagga atagcgataa gcggcccaac aagtttttgc
    19321 ggcccgctgg tactttggcg aatcgcatct tccacatcgc tacggtaatc ggcacgttcg
    19381 acaatcacct gccgaatcag cattatcgga atcaacaaca gcaatactgc accaaacagg
    19441 gtggtcattt tccagaacag ggaggatttc aacatagtct tctcctttgc aatggcagca
    19501 ggatacgaag gctatgtggg aagaatttga agctatgtga agtgacggtg aagtcgaagc
    19561 gaggccagca cgccaccttc ctgcacgttg cgcagcgtga cttcgccgtt aaacaaacgg
    19621 gcgacctcac tgacgaacgc caaccccaga ccgctgcttt tttgcccatt tgcacgcggc
    19681 aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt caggaatacc actaccggta
    19741 tccagcacct taagcgtgac gtgttcctga tccacttcgg cgcttagcgt tatacgaccg
    19801 ctctcggggg taaaatcgat ggcgttatcc agtaaattcc ccagcgcctg atccagtaac
    19861 gccggttcag cagcaacatt aacctcggtg ggcgtaacat gtagagtgat gtttttttct
    19921 gccaactgca cggtgcgcgc ttcgctgacg cggcggaata atgccgccac atcaacagca
    19981 gtcaaaacga cttcctgacg attctccagt cttgcctggc gtagtaacgt ttccaccagt
    20041 gcctgcattc gcgcattttg cgtcagaatg ttgtcagtaa aacgagccac tacttcaggc
    20101 ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg ccgccagtgg gctttttagc
    20161 tcatgggtta acgcataaac atactgctca atatagtttt tcccttccag cttcacgcgc
    20221 atactttcca gcgcctgcgc gagtttacgc agctcgctac taccgagatc gggaagagga
    20281 acgggcttat tgtcagtgac ggaatcagca tagcgagtga gccgggcaat ggagcggttg
    20341 atccaccaaa ccatgcctgc gccaatcacc agtgcgatcc ccaacaaaat ggcgctggcc
    20401 cataaaattc gccgctcgct gcgtttaatg accggagcca tcgccgcgtt cggtttgcct
    20461 acgctcaaaa cgccaataag ccgcgagccg tccataatcg gcgcggcaac atacatcacc
    20521 gaactttcgg gatcggcagg attttgcaac gtgctacgcg caccatactg accacgcaac
    20581 gttagccaga cgtcattcca gcgcgaataa tcctgcccaa cggctttatt tgccgaatcg
    20641 aacaatactt tgccctgcgc atcggtcata tagacatgat attcattgcg cactttgtta
    20701 atgccaccga tattggcgcg aaacgggcga tgttgtagct gattaaacgc ctgcgccagt
    20761 tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg ccagctccgc cagcaacgtt
    20821 gcggtatcga ttaacgtccc ctccgttgct cttcgcacgc ccggtttaac ttctttgaca
    20881 aaaatagcca gtacgaacca ggccgccacc gccaccagta aaaaatagcc cagcagcaac
    20941 cgcatgccga tacgcattac aggcccctca ggctatatcc catgccgcga tgagtattaa
    21001 tcggagaaag atcggggttg atggcgcgca gcttggcacg caacgtttta atgtgggtat
    21061 cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac gctatccatt agttgctggc
    21121 gggaccagac gcggcctggc gacttgagta acgtcttcag caataaaaac tcataccgcg
    21181 tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc gggttcattc aattcaaaat
    21241 gaccaatacg gatgacggga gacggcgacg agaacttctt cacccgacgc agtaaggtgc
    21301 gcaccctggc gcacacttcg cggggtgaaa acggtttagc cacatagtcg tcagcgccaa
    21361 tttccagccc aagcaggcga tcgacctctt cacttcgggc cgtcaggaac agtacaggta
    21421 acgccggatg gagcgccagt aactggcggc acaattcaaa gccgctgata tcaggcaaac
    21481 caacatcgag aatcatgacg tcgggaacct gctggcgagc tttatccagc accggcaagc
    21541 ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa catgtagacc agcgtgtcgg
    21601 ctatcccttg ctcatcttcc actaaccaga ccgtttcccg ttgcataaat cgcctctgtt
    21661 attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt tttcggcgaa ccttctacaa
    21721 ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt ggcgtcataa aagcgcacga
    21781 cctgtaacga cttaaagacc agggacgtgc gttttttgaa tactacctcg ccctgagctt
    21841 tgccgttttt aatacgatcc gacagttcaa tcggcccgac ttgctgacaa gaaatagccg
    21901 catcggaggt atcttccgcc agacccaatc cccctttaat accacctgtt ttcgcccggc
    21961 tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa ggcttccaca acaattttgt
    22021 gatccgggcc gatcatttta aatacggtgt cgaccgaacc aatctcttct gcatgagcca
    22081 atggccccag cattatcagg cttaaagaca ggatcaaatg cttgtatttc atattgttac
    22141 catttttatc tattacacga gcaattagtt cgtcacggta aataacatat tgattaagaa
    22201 caaaatctca atacttttat ttccgtcaag ccctaaaaca tactctacta agaaaaaaac
    22261 actgaatgct aaaacagcaa aaaatgctat tatccaatta cctgatgtca ggtgctcgtt
    22321 gttgaaagga tgaggatatt ttatggatca ggccggcatt attcgcgacc ttttaatctg
    22381 gctggaaggt catctggatc agcccctgtc gctcgacaat gtagcggcga aagcaggtta
    22441 ttccaagtgg cacttacaga gaatgtttaa agatgtcact ggccatgcta ttggcgcgta
    22501 tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta cgcctgactg cgcgtccgat
    22561 tctggacatc gcgctgcaat accgcttcga ctctcaacag acatttaccc gcgcattcaa
    22621 gaagcagttt gcccagactc ctgcacttta ccgccgttct cctgaatgga gcgcctttgg
    22681 tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca gagcacaaat ttgtcaccct
    22741 ggaagatacg ccgctgattg gtgttaccca gagctactcc tgttcgctgg agcaaatctc
    22801 tgatttccgc catgaaatgc gttatcagtt ctggcacgat tttctcggca acgcgccgac
    22861 cattccgcca gtgctctacg gcctgaatga aacgcgtccg agtcaggata aagacgacga
    22921 acaagaggta ttctatacca ccgcgttagc ccaggatcag gcagatggct atgtactgac
    22981 ggggcatccg gtgatgctgc agggcggcga atatgtgatg tttacctatg aaggtctggg
    23041 aaccggcgtg caggagttta tcctgacggt atacggaacg tgcatgccaa tgctcaacct
    23101 gacgcgccgt aaaggtcagg atattgagcg atactacccg gcagaagacg ccaaagcggg
    23161 agatcgccca attaatctgc gctgtgaact gctgattcct atccgccgtt aacgctgcag
    23221 ctcatctaat gcaggggcgt ccagatgcga gatgtcccct gcggtttcaa cgacccagcc
    23281 agacgccagc cacaggcttt cctgataatc cacgcgcgaa atagaacagt tacgcagacg
    23341 taagcggcgc tctgcccatg ctggtaatcc gagaatcgta ctcaccagac atcccagtgc
    23401 aataccatgg ctcaccagca gcggtcggct tccctgcggt aagtcccggc aagattccag
    23461 tgccgcattg acgcgatcgc tgagctcctg cattgactcg ccttcaggaa tacgcccgtc
    23521 aacggtgcca ttgaccagct gccgacgcca gttctcttct tcttcggtca gagaatcgat
    23581 atgtcttttt tccagcacac ccatgtttaa ttcacgcagg cgagaatcaa agatgatgtc
    23641 acagccgcag gcctgggcga tgatttccgc cgtacgccgg gtgcgtccta aatcgctact
    23701 gataatatgc gtaatgccaa gctctttggc acgggttgcc acctgcatcg cctgttgctc
    23761 acctttggcg gtcagcgggc tgtcagactg gccctgaata cgtcgctcgg cgttccactg
    23821 cgtttcaccg tggcggacta ggtatacctg taacatgctt tttttccgta tactgctgta
    23881 acgttaattt tctgagtaat gctgattatg caccaagtta tctgtgcgac caccaatccc
    23941 gctaaaattc aggccattct gcaggcattt cacgagatct tcggcgaagg atcctgccat
    24001 attgcatccg ttgccgtcga gagcggtgta ccggaacagc cctttggcag tgaggaaacg
    24061 cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt tacttccaga ggctgatttt
    24121 tgggtggcga ttgaagctgg catcgatggt gacagcactt tcagttgggt ggtgattgaa
    24181 aacaccagcc agcgcggcga ggcgcgttcg gctactttgc cgttacctgc ggtgattctg
    24241 cagaaagtgc gtgaaggtga ggcgcttggt cccgtgatgt cgcgttatac cggtattgat
    24301 gagattggtc gtaaggaagg ggcgatcggc gtgtttaccg ccgggaaact cactcgcgcc
    24361 agcgtctatc accaggcggt gatcctggcg ctgagtccgt ttcacaatgc cgtgtatcag
    24421 gcattgtagg acggataagg cgttcacgcc gcatccggca ttcggtgcac gatgcctgat
    24481 gcgacgctgc cgcgtcttat caggcctaca aaatcaatcg cttttcagca acacctcttc
    24541 cagccactgg cgcagctcca cgggcgcggc tttcaggctg ttagatccac gcgtaatcgt
    24601 cgcgatgccc gcgccgagtt cattttttaa ctcacgctga ctcatttcgc cacgcaacag
    24661 ctcttcgaca atacgcacgc gagtccccaa cgcttcgcgc tcatctggcg tcagcatcag
    24721 gtttaacaac ggtaaatgga gatcgttttg gtaggcattc ttaagcaggt cgacaaaacg
    24781 taaccactcc tggtgacgct gttctgccat cgctgctgaa tagggtgatt gttgggccat
    24841 aatatgtcgc cattgttagc gggggaagca aaatgcctcc cccggttgta ctcgctaaag
    24901 agtacgatag catatcataa acgtgcggat cagtaacgac gtccccattc cgtggcgctc
    24961 atcaacgtcg gtttatcccc catgaaataa cggtagtaag cgtcataagc cagcacgttc
    25021 ttcacataac cgcgcgtctc ggagaacgga atactctcga caaatgccac agcgtcgata
    25081 cgcccggcgc tgttgccaag ccaggttcgc acccgtccag gtccggcgtt ataagctgct
    25141 gaggagaaaa tacggttatt gccaaactgc tgataaacat attgcaggta actggtgcca
    25201 atgttgatat tcgtttccgg atccagcaat tgcccagggc tgctataacc aggaatagag
    25261 aacatcttca ccgtatgggt cgctgtacca ggcataatct gcatcaggcc gctggcccct
    25321 accggtgatt tcactttcgg attccaggcg ctctcctgac gggcaatcgc catcgcatag
    25381 ctttgcggga tctctttccc actggtgtaa cgtttgaaaa gatcgttgta agccagcggg
    25441 aatcgctctt ccagatgatc ccaaagcttc ccggcgatcg ttgcctgaac gctaagatcc
    25501 caccattggt tattgaaagc ataccgagcc agttgagcct gctctgtttt tgacttgctc
    25561 ttcaccagat tggcccactc gctacgcgcg gtgttatcga gattccagta catcaactcg
    25621 cgcacgcgcg ccatctccgg cccctgagtc agggcgctgt caacattctg cggcgcttta
    25681 tcaatcttca gctcatactc ttctccgatg cgttgtgctg caaccatcgg gtagaaacca
    25741 cgctgttgca tgagttgatg caaaatctct ttcgcttcag cttcacgtcc gcgttccagc
    25801 aataaatcca cctgccagta acgccattca tctttctctt ttgcttccat cggcagacgc
    25861 gccagccagg tattcaggcc gcgacgatcg ccggtgccaa gtgccattcg cacacggcgt
    25921 tcaatcaacg acgtggattg cgagcgcata atgacgtcat cgcgccattt cgcctgctcg
    25981 tcggtgacat cgttgcccat caaacgccag gcgacgatat cgcgcagctc ctgaatctga
    26041 tcttcattaa gctgctgcac ctgggcaagc gacgggatca tcagccgtgc attttcagca
    26101 tcctgccgcg ccacactggc aaacgccacc gctgccattt gacgggtaaa atcggtcgct
    26161 ccggttgtac gcgcgaaggt cagtaccgta ttagggttgt tcgccagtga aatgattgcc
    26221 gaggcgatag tctggtaatc ggcaggcatc tgccctgcca gcactgttac caggccagtg
    26281 ttacccgctt tcatcgccag acggatacgc tctaaatacg ccagcggatc ttgtttacct
    26341 gacgcacgcc agacgctaaa taacttgtca caggcgttag gctggctctt gccggttagc
    26401 cacagctctt tcgccccttg ccaggcttct tcactctgcc cggtgttcca tttcgcatag
    26461 tagtaattac attgcgcttc ggtagttccg ggcttttccg ggctaaaggc taacaagcca
    26521 cgccagtctt cacgccgcgc cagttcattg acgaaacgag attgcagcgt gcgagcggga
    26581 ggaagcgtgg ggttagcgcg aacaaagtta gtgaccgtca ccgccggttg attcatcaga
    26641 tcatcggtga tctggcggta ttccaggtag ggataaagcg gataatcctt cagtccaggc
    26701 atcatttgtt cgaccacatc catttgtcga ttatcccagg cctgcttgat ttgcgcgtaa
    26761 cggctacgct gctcatccag tgagtcggct cgcgccacgc tgctgaccgt cagcagacag
    26821 acaccggcag ccaacagccg ccaggtaact tgtttggctt tttccacaag cgcatcctct
    26881 aagtgtaaat acatcaatgc agcgtcatgc cgcgtaatag atttatgcta accagtcatt
    26941 gccgtttacg ccacgttacg gacacttttt tacttttact gcgaaggtga tcgaccaact
    27001 gggattaggc ggggaaaaag gctacacttc gcctttgaaa actcatcatc acgataaaca
    27061 tagaggcgaa gtccaacgtg gctcaattcg tttataccat gcatcgtgtc ggcaaagttg
    27121 ttccgccgaa acgtcatatt ttgaaaaaca tctctctgag tttcttccct ggggcaaaaa
    27181 ttggtgtcct gggtctgaac ggcgcgggta agtctaccct gctgcgcatt atggcgggca
    27241 ttgataaaga catcgaaggt gaagcgcgtc cgcagccaga catcaagatt ggttacctgc
    27301 cgcaggaacc gcagctgaac ccggaacaca ccgtgcgtga gtccattgaa gaagcggttt
    27361 ctgaagtggt taacgccctg aaacgcctgg atgaagtgta tgcgctgtac gccgatccgg
    27421 atgccgattt tgacaagctg gccgctgaac aaggccgtct ggaagagatc attcaggctc
    27481 acgacggtca taacctgaac gtacagctgg agcgtgcggc ggatgcgcta cgtctgccgg
    27541 actgggacgc gaaaatcgct aacctctccg gtggtgagcg tcgtcgcgta gcgttgtgcc
    27601 gcctgctgct ggaaaaacca gacatgctgc tgctcgacga accgaccaac cacctggatg
    27661 ccgaatccgt ggcctggctg gaacgcttcc tgcacgactt cgagggcacc gtggtggcga
    27721 ttacccacga ccgttacttc ctcgataacg ttgcaggctg gatcctcgaa cttgaccgcg
    27781 gtgaaggtat tccgtgggaa ggcaactact cctcctggct ggagcagaaa gatcagcgcc
    27841 tggcgcagga agcttcacaa gaagcggcgc gtcgtaagtc gatcgagaaa gagctggagt
    27901 gggtacgtca gggaactaaa ggccgccagt cgaaaggtaa agcacgtctg gcacgctttg
    27961 aagagctgaa cagcaccgaa tatcagaaac gtaacgaaac caacgaactg tttattccac
    28021 ctggaccgcg tctgggcgat aaagtgctgg aagtcagcaa cctgcgtaaa tcctacggtg
    28081 atcgcctgct gattgatgac ctgagcttct cgatcccgaa aggggcaatc gtcgggatca
    28141 tcggtccgaa cggcgcgggt aaatcgaccc tgttccgtat gatctctggt caggaacagc
    28201 cggacagcgg caccatcact ttaggtgaaa cggtgaaact ggcgtcggtt gatcagttcc
    28261 gtgactcaat ggataacagc aaaaccgttt gggaagaagt ttccggcggg ctggatatta
    28321 tgaagatcgg caacaccgag atgccaagcc gcgcctacgt tggccgcttt aactttaaag
    28381 gggttgatca gggtaaacgc gttggtgaac tttccggtgg tgagcgcggt cgtctgcatc
    28441 tggcgaagct gctgcaggtt ggcggcaaca tgctgctgct cgacgaacca accaacgacc
    28501 tggatatcga aaccctgcgc gcgctggaaa acgccctgct ggagttcccg ggctgtgcga
    28561 tggttatctc gcacgaccgt tggttcctcg accgtatcgc cacgcacatc ctggactacc
    28621 aggatgaagg taaagttgag ttcttcgaag gtaactttac tgagtacgaa gagtacaaga
    28681 aacgcacgct gggcgcagac gcactggagc cgaagcgtat caagtacaag cgtattgcga
    28741 agtaatgcgt aaaatgccgg atgcggcgtg aacgccttat ccggcctaca aaccaagaaa
    28801 tttcaatacg ttgtgaatct ccaggcctga taagacgcgg taagcgtcgc atcaggcatt
    28861 gagcaccgaa tgccggatac ggcgtgaacg ccttttccgg cctacaacag ccttagtatc
    28921 cgcaatttcg tagcgggtca cccgtgcagg ttttttcaaa ccttgtttaa tctcgacagc
    28981 ctcttccaga gaggttttca actcatcaaa aaacttattt tatacctcgc ctttgagccg
    29041 tttcatcgcg gttatctctg ctcccccatc atctcccgca ccagctccac gcagcgcagg
    29101 aaacggctgt cataatcgtc ctcttcaacc cgcacgaatt cgatattgtt ctcttccagc
    29161 atctccacca gcaagttctg gaactctttg cgatccaccg aactgccgag gctgcgtaaa
    29221 ccatccgcca cccacggcgt gttgttctcc agcaggatca ccagatcgaa acggtattca
    29281 tcaatcaacg cctgtacgaa cggatgctca cgcccttcgt actttttgca gaacgcctgg
    29341 gtagtgacaa aatcagtgtc aataaacgcc actttattgg catatttcac tgcaaaatca
    29401 atgtattgtg cgtggcccag cgcgatttta tcgtaatcgg aatactgcaa tgcgatctca
    29461 tcaccgccga ggtgtgaaaa gacataatcg cgaccatatt cccacgcact ggtggtattg
    29521 aagatattgg caagtttgtt taccagggtg gatttaccgc tcgactcgcc accaaggatc
    29581 gccacggtac gtacaaagaa cggcttcact tccgtaggaa tatattccca gtagcggaac
    29641 gggttttcgc ggatctgcgc accgctgata ctcataaagg tacgtttcgg atcgaccagc
    29701 accgtatcga tccccagatg ttccatatac tgtggcgcat cggcttcttc cgaggtgtag
    29761 atcaggtccg gctgaatccc cttttcagcc ataaactttt tgatgccgtt gctccacaca
    29821 tcccagccgt gcggatacgg ctccatgccc tcttcgttga aagcatgaat gcgaatattt
    29881 ttctgatatt taaaagtttg taataaccag cgcagacgat ccggcaccgt gggctgctgc
    29941 gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat cgtcaaaacc cataatgata
    30001 tgcagctcgt caacctggct acaggcgcgc tggataaggt agatatgtcc ggtatgcagc
    30061 gggtagaact taccgaatac gacaccaatg gttttcttct gtcggggaaa ttcaagcccc
    30121 aaaaaacggt gcaacgcctc cagcttttgc gcgctggggc ttttaatttt ggcattcagt
    30181 aactggctta aatacccttt ggtcataccg ctggcatcag ctacctgctg tagcgtgcag
    30241 ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg acatatctcc ctccgtatct
    30301 ctcattataa gtcgtcgaac acgctaagcg cgtcggagag ttttttaacg ccaaaaatct
    30361 gcatcccttc cggcgctttt ttcggtacgt tagccgccgg aacaatcgcc cggcgaaaac
    30421 cgtgtttcgc cgcttctgaa attcgttcct gaccgctggg caccgggcgg atctcccctg
    30481 ccagcccgac ttcaccaaac accaccagat cctgtggcag cggtctgtca cgcaggctgg
    30541 aaaccatcgc cagcagtaac gctaaatcgg cactggtttc ggttaccttc acgccgccga
    30601 ccacgttcac aaatacatcc tgatcggcca tttgcagacc accgtgacgg tgcaagacag
    30661 ccagcaggat tgccagacgg ttttgttcca gccccactgc cacgcggcgc ggattcgcca
    30721 tcatcgagtg atcgaccagc gcctgaatct ccaccagcaa cggacgcgtt ccttcccaca
    30781 ccaccatcac ggagctgccg gaggtcactt catctccgcg acttaagaaa attgccgaag
    30841 ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa gacgcccagc tcattcaccg
    30901 cgccgaagcg gtttttatgg ctgcgcaagg tgcgaaaacg ggagtcggca tcaccatcca
    30961 gaagcaccga acagtcgata cagtgttcca gcactttagg gccagccagc gagccatctt
    31021 tggttacgtg cccaaccatg acaatcgcca caccgcgcgt tttggcgaag cgcgtcagat
    31081 aagccgccgt ttcacgcacc tgcgccacgc taccaggcga tgattgtaca tccgccatat
    31141 gcatcacctg gatcgagtca attaccatca gtttcggttg ctcttcttcg gcaatcaggc
    31201 agatctgttc gatgctggtt tccgacaaca tattgagatt gtcagtcggc aggccaaggc
    31261 gatgagcgcg cattgccacc tgttgcagcg actcttcgcc agtgacatac agcgttttca
    31321 tctgctgggc cagtttgcac agcgtttgca gtagcagcgt ggatttcccc gcacctgggt
    31381 taccgccaat cagaatggcg cttcccggca ccacgccgcc gcctagtacg cggtcgaact
    31441 ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct gatatcggag agtttctgga
    31501 cttttgccac cccggcgcta ccggcatagc cgctgagacg ctcgttacgc gccaccgttg
    31561 gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc atgacaggca ctgcactgcc
    31621 cctgccagcg cggataatcg gccccgcatt cattacaaac aaaggcgcgt tttggagctt
    31681 ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg atggcgggcg agcaattact
    31741 tctgattcag gctgccagag aggatgcaga atacccccat caggtcagcg tgacggatgg
    31801 tgacttccgc cttttcattc acttttggct tggcatggta ggcaatcccc agccctgctg
    31861 ctttgatcat cggcaggtca ttagctccat cgccaatcgc cacggtctgc gccagcggga
    31921 tttcatactc ctgcgcgagg cgagtcaaag ttttcgcttt gtactgcgcg tctacgatgt
    31981 cgccgatcac attgccggta aatttaccgt ccatgatctc cagttcattg gctaccacgg
    32041 cggtcaggcg cagcttgtcg cgcagatatt cagcaaagaa agtaaagccg ccggaggcaa
    32101 tcgccacttt ccagcccagc gtttccagct tgagcaccag ttgcgttaag cctggcatca
    32161 gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc agcgcctttc agcgtcgcca
    32221 cacggctgcg caggctggcg gtaaaatcga gttcgccgcg catcgcccgt tcggttactt
    32281 ccgccaccat ctcgcccgtt ccggccagtt tggcaatttc atcaatacat tcaatctgga
    32341 tggcggtgga atccatgtcc atcaccagca aacccggcgt gcgcaggtgc gggattttcc
    32401 ccagcggggc gacatccagt tgcgcttcat gggccaggcg cgtagcccgt gcggtgagtg
    32461 aacctgccag acgaatcacc tgataatctt ccacgcacca ggcggcaaca atcaccatcg
    32521 ccgcacccag tttgctctgg tattgggtca gacgctgttt atccagccca cgaccataca
    32581 gcagccagcc gctacgacct gcgtggtaat ccagtggcat cacttcatca ccacttaatg
    32641 aaagaggcag acccggccat aaagagacat cttcaggcag gtcgcaccag gtaatgttag
    32701 gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat aacgcatgag gctaccttgt
    32761 atccattgct tctggcaaca ttaagtctca aattttcaaa gggtggaaga tggctcgcac
    32821 aaaactgaaa ttccggctgc atcgggcagt gattgtcctg ttctgtcttg ccttgttagt
    32881 ggcactgatg cagggggcgt catggtttag tcaaaaccac cagcgacagc gtaatccaca
    32941 gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg ctgaacgttg caccgctgat
    33001 gcgtaccgac tcaccagatg aaaaacgcat tcaggcgatc ctcgatcagt taacggatga
    33061 aagccgcatc ctcgacgcgg gtgtgtatga cgaacaaggc gatcttatcg cccgttcagg
    33121 cgaaagcgtc gaagtgcgcg accggctggc gctcgacggt aaaaaagcag gcggctattt
    33181 taaccagcag attgtcgagc caattgcggg taaaaacgga ccgctcggct atctgcgcct
    33241 gacactcgac acccatacgc tcgccaccga agcccaacag gtggataaca ccactaacat
    33301 tttacgcctg atgttgctgc tctcactggc aatcggcgta gtgctgaccc gcacgctgct
    33361 acagggtaaa cgcacccgct ggcagcaatc gcccttcctg ttaaccgcca gcaaaccggt
    33421 gccggaagag gaagaaagcg agaaaaaaga gtgacccatt actacaagaa aggaaatcgt
    33481 tatgtccaca ttacgcctgc tcatctctga ctcttacgac ccgtggttta acctggcggt
    33541 ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc gttctgtttc tctggcgcaa
    33601 tgccgacacg gtagtgattg gtcgcgcgca gaacccgtgg aaagagtgta atacccggcg
    33661 gatggaagaa gataacgtcc gcctggcgcg gcgcagtagc ggtggcggcg cggtgttcca
    33721 cgatctcggc aatacctgct ttacctttat ggctggcaag ccggagtacg ataaaaccat
    33781 ctctacgtcg attgtgctca atgcgctgaa cgcgctcggc gtcagcgccg aagcgtccgg
    33841 acgtaacgat ctggtggtga aaacagtcga aggcgaccgc aaagtctcag gctcggccta
    33901 tcgcgaaacc aaagatcgtg gcttccacca cggcactttg ctgctcaatg ccgaccttag
    33961 ccgcctggca aactatctca atccggataa aaagaaactg gcggcgaaag gcattacctc
    34021 agtgcgttcc cgcgtgacca acctcaccga gctgctgccg gggatcaccc atgagcaggt
    34081 ttgcgaggcc ataaccaagg cctttttcgc ccattatggc gagcgtgtag aagcggaaat
    34141 catctccccg gacaaaacgc cagacttgcc aaacttcgcc gaaacctttg cccgtcagag
    34201 tagctgggaa tggaacttcg gtcaggctcc ggcattctcg catctgctgg atgaacgctt
    34261 tagctggggc ggcgtggaac tgcatttcga cgttgaaaaa ggccatatca cccgcgccca
    34321 ggtgtttacc gacagcctca accccgcgcc gctggaagcc ctcgccgggc gactgcaagg
    34381 ctgcctgtac cgcgcggata tgctgcaaca agagtgcgaa gcgctgttgg ttgacttccc
    34441 ggaccaggaa aaagagctac gggagttgtc gacgtggata gcgggggcgg taaggtaact
    34501 cgctgattaa aataccaaag gaagggaata tgatcgatct ggaaaaccag gagcgggaga
    34561 ttatcaattt aatgttaagc cagcgtattt cctggctagc cgccgtgcgt atcaggcata
    34621 aattatcgct tgctgaagta agtaaaatgt taggtatctc gattaattct ctaaaataga
    34681 tcgaaaagac ggaacggctt agctcaaaca tcaaaagcaa aatgggagaa atttatggtt
    34741 gtccaccaga gttgctgata tgcccttcct ggatgacggc tgaacataaa tgaatagatt
    34801 cgtgaataga tacttgaatg tattttattt tttatccata tgaaaaataa tagtaaatct
    34861 ataaatcagt tgctattgat gaatagatac taaaaagccg gagcagtccc ccactccggc
    34921 ttaacacgac aatcgccttt cagcgaaaca caattactct ttatcgccca gcagaacgga
    34981 ttccagtgcg attttgatca tgtcgttgaa ggtggtctga cgctcagcgg cagtggtctg
    35041 ctcgtgagtg cggatgtggt cagacacagt gcagatggtc agggctttcg cgccaaattc
    35101 tgcagcgacg ccgtagatac cagccgcttc catttccacg ccgaggatgc cgtatttttc
    35161 catcacgtcg aacatttcgc cgtccggaga gtagaacagg tcagcggaga acaggttacc
    35221 aacgcgagca tcaatgccca gtgctttagc cgcgtctacc gcgttacgca ccatgtcaaa
    35281 gtcagcgata gcggcaaagt catgatcttt aaaacggatg cggttaactt tggaatcggt
    35341 gcaggcaccc ataccgataa cgacgtcgcg cagttttacg tgcggcagaa ctgcgccaca
    35401 ggaacccacg cggataattt tcttcacgcc gaaatcggtg atcagttctt tggtgtagat
    35461 ggagcaggac gggataccca taccgtgacc cattacggaa attttgcggc ctttgtaagt
    35521 accggtgaag cccagcatac cgcgaacgtt gttcacttca cgggcatctt caaggaaagt
    35581 ttcagcaata tacttcgcac gcagcgggtc gcctggcatc aaaactacgt cagcgaaatc
    35641 gcccatttct gcattaatgt gtggggtagc cattgtttta tccttatcaa catgttttca
    35701 ccctctccct gagggagagg gccggggtga gggcatcaag ccgcacccat tatcagaaca
    35761 tggctttgcc atattccata tcagaagtac caaaatattt tgccagagtc tggccgatat
    35821 ccgcgaaggt ttcacggtga cccagtgagc ccggttttac tttcgggccg taaaccagta
    35881 ccggaatgtg ttcacgcgtg tggtcagtac cggtccaggt cggatcacaa ccgtggtcag
    35941 cagtgaggat caggatgtcg tcatcgcgca gcagagacat cagctccggc agacggcggt
    36001 cgaacagctc cagacccaca gcataaccgg cgacgtcgcg acggtggccc caggaagagt
    36061 cgaagtcaac gaagttggtg aagacgatgg tgttatcacc cgcttctttc atctctttga
    36121 tggtggcgtc aaacagcgcg tccaggccag ttgctttcac tttcttggtg ataccgcagt
    36181 tggcgtagat gtctgcaatt ttaccgacag aaaccacctg accgtgtttt tcatcaacca
    36241 gtttctgcag cacggtcggt gctggcggct caacagccag gtcgtgacgg ttaccggtac
    36301 gctggaagtt accggctttg tcaccgataa acggacgagc gataacacga ccgatgttgt
    36361 agccgccgtt ggtcagctct tcacgggcga tttcgcacag ttcgtagagt ttatccaggc
    36421 caaaggtttc ttcgtggcag gcaatctgga acacagagtc agcggaggta tagaaaatcg
    36481 gcttgccggt tttcatgtgc tcttcgccca gctgatccag aatgaccgta ccggaagagt
    36541 ggcagttacc gaggtaaccc ggcagattag cgcgttcaac cagtttatcc agcagctctt
    36601 gcgggaagct gttttcgtga tcggagaaat atccccactc aaatagaacc gggacaccgg
    36661 cgatttccca gtgaccagac ggggtatctt taccggatga catttcgtgc gcccatgcgt
    36721 acgcgccgat aacttcagcg ttgccgtcca ttcccgccgg aatgaaaccg gtagaacctt
    36781 cgtgtgcttt cgccagcccc agacgggtca gatttggcag attgagcgga cctttacgac
    36841 cgttatcagc ttcgcctttg gcacaagctt ctgcgatatg acccagggtg tcagccccga
    36901 cgtcaccaaa gcgttctgca tcttctgtag cgccgatgcc gaatgagtcc agcaccataa
    36961 taaatgcacg tttcatatgt tctccgtact cagtgcttca aatttaaatg atcagatcag
    37021 tataccgtta ttcgctgata cggcgataga cagttggtgt gctttccggt gctttatcgg
    37081 caagtttaat tgccgctttc accgctttcg ccgcttcctg ccagctgttt tcgtctttcg
    37141 cgtggataac cgccagcgga cgctgaccgt ctacctggtc gcccaaacgc gccatatcag
    37201 taaagccgac gctgtaatca atggtgtcag atgcctgacg acgaccgccg cccattgcaa
    37261 ccactgccat ccccagcgcg cgggtatcca tttcactgac aaaaccttcg gtatcagcat
    37321 agactgcttt cgtcagcatc gctgtcggca gatacttcgc gtagttctca acgaagtcgg
    37381 ttgggccttt ttgtgccgct accatacgac caaagacttc tgctgcttta ccgttgtcca
    37441 gcactgcttg caatttcgcg cgcgcttcgg cgtcatcttt cgccagtttg ccggagataa
    37501 gcatctccac gcacagcgcc atcgtgacat caaacagacg cgggttacga tactcacccg
    37561 tcaggaactg caccgcttca cgaacttcaa ccgcgttacc tgcactggag gccagtacct
    37621 gattcatgtc ggtgagcagc gcggtggtgc gcacgccagc gccgttagcc acgccaacaa
    37681 tcgcttcggc aagggcttca gagagttcgt aggtcggcat aaacgcgccg ctacccactt
    37741 tcacgtccat caccagcgcg tccagacctt ccgcaagttt cttcgccaga atagaggcgg
    37801 tgatcagcgg gatggagtcc acggttgcgg taatatcacg ggtcgcgtag aaacgtttat
    37861 ccgccggagc cagtgagctg gtctgaccga taatcgccac gccgacgtct ttaataattt
    37921 cgcggaaacg gttgtcatcc gggaaaatgt cgaagccagg gatggattcc agtttgtcga
    37981 gcgtaccgcc agtatgaccg aggccgcgac cagagatcat cggaatatag ccgccgcagg
    38041 ctgcgaccat cggccccaac atcagcgaag tcacatcgcc gacgccacca gtggaatgtt
    38101 tatcaacaat cgggccattc agatgcaggc ttttccagtc gagaacggtt cctgaatctc
    38161 gcatcgccat ggtcagcgag acacgctcag gcattgtcat atcgtggaag aaaatggtca
    38221 tcgcgagggc ggcaatctgc ccttcggaga tagtgttgtc gcgaatgccg ttgataaaga
    38281 aacgaatttc ttcatcgctc agtgcatgac catcacgttt tttacgaata atttcttgtg
    38341 cgagaaacaa ggtaacctcc cgaggaaaag agtatgaggc ggcttttgcc ggatgcgtct
    38401 acgccttatc cggcctacgg gtagatacgt ctcgtaggcc tgataagcgc agcgcatcag
    38461 gcgtaaagca tcttacttag tagctgctgg cgctcttacc gtcaccgtgg cccagcgctt
    38521 tcaacaggct tgccagcagg ctggaagcac caaagcggta gtgacgcgca tctgcccagt
    38581 cagcaccgaa cagttcatct gcgatggcga gatatttctg cgcatcttcc gcagtacgca
    38641 cgccgcccgc tggtttgaaa ccaacggttt tttctacgcc catatcacgg atcacttcca
    38701 tcatgatgcg cgcgctttcc ggcgtcgcgt tcacagccac tttaccggta gaggttttga
    38761 tgaagtccgc acccgctttg atggagattt cagacgcttt acggatcagc gcttcgtctt
    38821 tcagttcgcc ggtttcgatg atcactttca gcagtacatt cgctgccgcg caagcctctt
    38881 tacaggcttt caccaggtca aaaccaacct gctcgttacc cgccatcagc gcgcggtacg
    38941 ggaacacaac gtcaacttca tcagcaccgt aggcgattgc cgcacgggtt tctgccagcg
    39001 cgatttcgat gtcatcgtta ccgtgtggga agttggttac cgtagcgatt cggatttccg
    39061 gggtgccctg ctctttcagc gttttgcgag caatcgggat aaagcgagga tagatacaga
    39121 tagcggcggt attgccgacc ggggttttgg cctgatgaca cagagcaatt actttctcgt
    39181 cggtgtcgtc gtcattcagg gtggtcaggt ccatcaattt cagtgcacgc aggctgcttg
    39241 ctttcagatc agtcatttca ttctcctggc ttgtcgccaa taaaattcac cttgcgagtt
    39301 tgttagtatt ctaacatcta ctccgcaaca cacttcgata tacatcacaa ttaaggaaat
    39361 ctacttacaa gtttgcatca ctgtaatgcg atctggttca aataattcac tttcaaatga
    39421 atgcgtcagt ggtggcaaac gcatcaggat caaagtgaac atcacgaaac ttcttacaat
    39481 ggcgcatcac cttcggcata aaaggaaaag aagatgccca cttctcatga aaatgcactg
    39541 caacaacgtt gccagcaaat tgtcaccagc ccagtgctta gcccggagca gaagcgccat
    39601 tttctggcac tggaagcaga aaacaatctg ccttatccgc agcttcctgc cgaagcccgc
    39661 cgcgcgctgg atgagggtgt aatctgcgat atgtttgaag gtcatgcgcc gtacaaaccg
    39721 cgctatgtct tacccgatta cgcccgtttt ctggcgaacg gttccgaatg gctggagctg
    39781 gaaggcgcga aagatcttga tgacgcactc tctctgctga ccattcttta ccaccacgta
    39841 ccgtcggtca catcgatgcc ggtctacctg gggcaactgg atgcgttgtt gcaaccatat
    39901 gttagaattc taacacaaga cgagatcgat gttcgaataa aacgtttctg gcgttacctc
    39961 gacagaaccc tgccagacgc ctttatgcac gccaatatcg gcccgtctga ttcgcccatt
    40021 acccgcgcga tcttacgtgc agacgcagag ttgaagcagg tttcaccgaa cctgaccttt
    40081 atctacgatc ctgaaatcac ccctgatgac ctgctgctgg aagtggcgaa gaacatctgt
    40141 gaatgtagca aaccgcacat cgccaacggt ccggtgcatg ataaaatttt cacaaaaggg
    40201 ggctacggga ttgtgagctg ttacaactca ctgccgctgg cgggtggtgg cagcacgctg
    40261 gtacgcctta acctgaaagc cattgccgag cgcagcgaat cgctggatga cttctttacg
    40321 cgcactctac cgcactactg ccagcagcag atcgccatca tcgatgcgcg gtgtgaattc
    40381 ctctatcaac aatcacactt ctttgagaat agcttcctgg tgaaagaagg gctgattaac
    40441 cctgaacgtt ttgtgccaat gtttggcatg tatgggctgg cggaagcggt taacttgctg
    40501 tgtaagaaag aagggattgc cgcgcgctac ggtaaagaag ccgccgcaaa tgaagtgggt
    40561 tatcgcatca gcgcgcaact ggcggagttt gtcgccaata cccccgtgaa atatggctgg
    40621 caaaaacgcg ccatgttaca cgcacagtcg gggatcagtt ccgatatcgg caccacgccg
    40681 ggcgcgcgtt tgccgtatgg tgatgagcca gatccgatca cccatctgca aactgtcgcc
    40741 ccgcatcatg cttattatta ttccggcatc agcgacattc tgacgctcga cgaaaccatc
    40801 aaacgtaatc cgcaggcgct ggtacagctt tgcctcggtg cctttaaagc cggaatgcgt
    40861 gaatttactg ccaatgtcag cggtaacgat ctggttcgcg ttaccggtta tatggtgcgt
    40921 ttgtcggatt tagaaaaata tcgcgccgaa ggttcacgca ccaacaccac ctggctgggc
    40981 gaagaagccg cacgcaacac tcgtattctg gaacgccagc cgcgcgtgat aagccatgaa
    41041 cagcagatgc gctttagtca gtaagattat ccccttctcc tgcgttgacg ggccaggcag
    41101 tcgtctggct ctgtttttgc agggctgcaa tctgcgctgc aaaaactgtc acaatccgtg
    41161 gacgatggga cgttgcaatg actgtgggga gtgcgtgcca cagtgtccgc atcaggcgtt
    41221 gcagattgtt gacggcaaag tggtgtggaa cgctgtggtt tgcgagcagt gtgatacctg
    41281 cctgaagagg tgtccgcaac atgccacgcc aatggcgcaa tccatgagcg tggacgaagt
    41341 gcttagccat gtccgcaaag cggtgctgtt tattgaaggg ataacggtaa gcggcggcga
    41401 agccacgacc caactgccgt ttgtagtggc gctgtttact gctatcaaaa acgatccgca
    41461 actgcgccac ctcacctgcc tggtggacag taacggcatg ttgagcgaaa ccggctggga
    41521 aaaattactc ccggtgtgtg acggcgcaat gctcgatctc aaagcgtggg ggagcgaatg
    41581 tcatcaacac ttaaccggac gcgataatca gcagattaag cgcagcatct gtttgcttgc
    41641 agagcgcggc aagctggcgg aactgcgttt gctggtaatt ccagaccagg tggattattt
    41701 gcatcacatc gatgagctgg cgacgtttat caagagactt ggcgatgttc cggttcgcct
    41761 gaatgcgttt catgcccacg gcgtgtatgg cgaggcgcaa agctgggcga gcgccacgcc
    41821 ggaagacgtt gagccgttgg ctgatgcgtt aaaggtgcgc ggggtgagcc ggttgatatt
    41881 tccggcgctc tatttgtgac atgccggata cggcctacgg ttcggcacag acttgtaggc
    41941 atgataagac gcgacaagcg tcgcatcagg cattatgcgc cgactgccgg atgcggcgtg
    42001 aacgccttat ccggcctacg gttcggcaca gacttgtagg catgataaga cgcgtcagcg
    42061 tcgcatcagg cattgtgcgc caactgccgg atgcggcgtg aacgccttat ccggcctacg
    42121 gttcggcaca gac
//
LOCUS       DAZPEP010000020        41117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZPEP010000020 DAZPEP010000000
VERSION     DAZPEP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.020"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(200..1057)
                     /gene="murI"
                     /locus_tag="ACHUT9_001054"
     CDS             complement(200..1057)
                     /gene="murI"
                     /locus_tag="ACHUT9_001054"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFR1347590.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1002..2846)
                     /gene="btuB"
                     /locus_tag="ACHUT9_001055"
     CDS             complement(1002..2846)
                     /gene="btuB"
                     /locus_tag="ACHUT9_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFR1347591.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     regulatory      complement(2911..3101)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3215..4315
                     /gene="trmA"
                     /locus_tag="ACHUT9_001056"
     CDS             3215..4315
                     /gene="trmA"
                     /locus_tag="ACHUT9_001056"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFR1347592.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4355..4714)
                     /locus_tag="ACHUT9_001057"
     CDS             complement(4355..4714)
                     /locus_tag="ACHUT9_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFR1347593.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4714..5364)
                     /gene="fabR"
                     /locus_tag="ACHUT9_001058"
     CDS             complement(4714..5364)
                     /gene="fabR"
                     /locus_tag="ACHUT9_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFR1347594.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5685..7085
                     /gene="sthA"
                     /locus_tag="ACHUT9_001059"
     CDS             5685..7085
                     /gene="sthA"
                     /locus_tag="ACHUT9_001059"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFR1347595.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7068..7985)
                     /gene="oxyR"
                     /locus_tag="ACHUT9_001060"
     CDS             complement(7068..7985)
                     /gene="oxyR"
                     /locus_tag="ACHUT9_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFR1347596.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(8252..9625)
                     /gene="argH"
                     /locus_tag="ACHUT9_001061"
     CDS             complement(8252..9625)
                     /gene="argH"
                     /locus_tag="ACHUT9_001061"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFR1347597.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9686..10462)
                     /gene="argB"
                     /locus_tag="ACHUT9_001062"
     CDS             complement(9686..10462)
                     /gene="argB"
                     /locus_tag="ACHUT9_001062"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFR1347598.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10470..11474)
                     /gene="argC"
                     /locus_tag="ACHUT9_001063"
     CDS             complement(10470..11474)
                     /gene="argC"
                     /locus_tag="ACHUT9_001063"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFR1347599.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11628..12779
                     /gene="argE"
                     /locus_tag="ACHUT9_001064"
     CDS             11628..12779
                     /gene="argE"
                     /locus_tag="ACHUT9_001064"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFR1347600.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13131..15782
                     /gene="ppc"
                     /locus_tag="ACHUT9_001065"
     CDS             13131..15782
                     /gene="ppc"
                     /locus_tag="ACHUT9_001065"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFR1347601.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            15965..17698
                     /locus_tag="ACHUT9_001066"
     CDS             15965..17698
                     /locus_tag="ACHUT9_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFR1347602.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17913..18764
                     /locus_tag="ACHUT9_001067"
     CDS             17913..18764
                     /locus_tag="ACHUT9_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1347603.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18751..19092)
                     /locus_tag="ACHUT9_001068"
     CDS             complement(18751..19092)
                     /locus_tag="ACHUT9_001068"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFR1347604.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19094..19972)
                     /locus_tag="ACHUT9_001069"
     CDS             complement(19094..19972)
                     /locus_tag="ACHUT9_001069"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFR1347605.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(19938..22234)
                     /locus_tag="ACHUT9_001070"
                     /pseudo
     CDS             complement(19938..22234)
                     /locus_tag="ACHUT9_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            complement(22285..22605)
                     /locus_tag="ACHUT9_001071"
                     /pseudo
     CDS             complement(22285..22605)
                     /locus_tag="ACHUT9_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            complement(22620..23699)
                     /locus_tag="ACHUT9_001072"
     CDS             complement(22620..23699)
                     /locus_tag="ACHUT9_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFR1347606.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24008..26509
                     /gene="ptsP"
                     /locus_tag="ACHUT9_001073"
     CDS             24008..26509
                     /gene="ptsP"
                     /locus_tag="ACHUT9_001073"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFR1347607.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26521..27183
                     /gene="fsa"
                     /locus_tag="ACHUT9_001074"
     CDS             26521..27183
                     /gene="fsa"
                     /locus_tag="ACHUT9_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1347608.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27194..28297
                     /gene="gldA"
                     /locus_tag="ACHUT9_001075"
     CDS             27194..28297
                     /gene="gldA"
                     /locus_tag="ACHUT9_001075"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFR1347609.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            28571..29188
                     /locus_tag="ACHUT9_001076"
     CDS             28571..29188
                     /locus_tag="ACHUT9_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFR1347610.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29215..30120)
                     /gene="yijE"
                     /locus_tag="ACHUT9_001077"
     CDS             complement(29215..30120)
                     /gene="yijE"
                     /locus_tag="ACHUT9_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFR1347611.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(30214..32394)
                     /gene="katG"
                     /locus_tag="ACHUT9_001078"
     CDS             complement(30214..32394)
                     /gene="katG"
                     /locus_tag="ACHUT9_001078"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFR1347612.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32723..33613)
                     /gene="metF"
                     /locus_tag="ACHUT9_001079"
     CDS             complement(32723..33613)
                     /gene="metF"
                     /locus_tag="ACHUT9_001079"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFR1347613.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            33689..33823
                     /locus_tag="ACHUT9_001080"
     CDS             33689..33823
                     /locus_tag="ACHUT9_001080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347614.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(33962..36394)
                     /gene="metL"
                     /locus_tag="ACHUT9_001081"
     CDS             complement(33962..36394)
                     /gene="metL"
                     /locus_tag="ACHUT9_001081"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFR1347615.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36397..37557)
                     /gene="metB"
                     /locus_tag="ACHUT9_001082"
     CDS             complement(36397..37557)
                     /gene="metB"
                     /locus_tag="ACHUT9_001082"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFR1347616.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAVGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37834..38151
                     /gene="metJ"
                     /locus_tag="ACHUT9_001083"
     CDS             37834..38151
                     /gene="metJ"
                     /locus_tag="ACHUT9_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFR1347617.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38335..38943
                     /locus_tag="ACHUT9_001084"
     CDS             38335..38943
                     /locus_tag="ACHUT9_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFR1347618.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39088..39763
                     /locus_tag="ACHUT9_001085"
                     /pseudo
     CDS             39088..39763
                     /locus_tag="ACHUT9_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39850..40172)
                     /locus_tag="ACHUT9_001086"
                     /pseudo
     CDS             complement(39850..40172)
                     /locus_tag="ACHUT9_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
     gene            complement(40174..41109)
                     /locus_tag="ACHUT9_001087"
                     /pseudo
     CDS             complement(40174..41109)
                     /locus_tag="ACHUT9_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT        10059 a        11305 c        10243 g         9510 t
ORIGIN      
        1 ggtgtgcata atacgctttc ccgctacaga gtcaagcatt ttttttgctt ttctctgttg
       61 agattctcag gagaaccccg ctgacccggc ggcgtgtttg ccgttgttcc gtgtcagtgg
      121 tggcgcatta tagggagtta ttccggcctg acaagaggaa atttaaaata attttctgac
      181 cgcgcaacat tcaaccaaat cagcctaaaa ctgccagttt ttcgagcgtt tcgaagccgt
      241 aacgctgtaa aacgggcaat aattgttcag cttctggcgt cattgccata caaaaggcaa
      301 tattcgcatc ggcagatttt gcatccgggg cttcatgttc taacaaccag gccgttcggc
      361 gagcaatcgc tgcgccagaa tccaccagcc gggttccctc tggcagcact tgtaacagtt
      421 cttcttgtag tagagggaaa tgggtgcaac ccaatacaac ggtatctggc ggctctttca
      481 ttcttaacca cgggcgtagg atacgtttta gtgcatccag agaaacatct tcgccatgta
      541 gcttcgcttc agccaactca accatctctg ccgagcccag catttctatc tggcattcat
      601 tagcgaaacg cgcgatcagc tcatgagtat aagaacgttt aactgttccg cgggttgcca
      661 gtaatccgac aatgccattt gccgtcagac gtgcagcagg tttaatcgcc ggcacgacac
      721 caacaaccgg gaagtcgaac ttttcgcgta atgcaggaag tgaaacggta ctggcagtgt
      781 tgcaagcgac cacagccagc gcaaggggat aacgttcttg caccgcggtg acaattgcca
      841 ccactcgctc aacaataaac gcttcgcttt tttcgccata cgggaaagcg acgttatcga
      901 aagcataaat gtaatggaga tccggtaaga gatgccggat ctcgtcatag accgacaacc
      961 caccgacgcc ggagtcaaac accagcacgg tgggacgtgg ttcagaaggt gtagctgcca
     1021 gacaaggtgt attcccgtcc tgcagtttgg tagccataga ctgtctcata atctttgtcg
     1081 aacaggttgg ctattttacc acgaactgtc aggtgagagg tgaccggata cgcaaccgca
     1141 agatcccaca agctcacacc gcccatttta acggtttgat aaggataaga tgagtaatcc
     1201 ttatcatagc gagtgcctaa atactgataa gtaatacccc agtcgaagtc atacaactgc
     1261 cagtcgagct ggtatttcac ctgctgttta gcacggcgta acaacggcgt gtcggtaatc
     1321 gcattgcgcg catcgacata atcataactc acagtatgcg tcagtggtcc ggtatcaaaa
     1381 ttggcggtcg cctcgacgcc cttaatccgc gctttccctt cgttgtaata tttcagggtg
     1441 tgatcatcat aatcgatcaa gtcactgaca tcgttacgat atccggaaat acgccagttc
     1501 accccagcgg ttaagccttc aaacgcgcct tcccactgtt tgcttttctc cgggtccaga
     1561 ttcggatttc cgtagaagcc atacagttgc cccagatttg gtgccttata agatgtcccg
     1621 taggaagcaa tgaagcgata accttcgatg aattcccaac cggcgctggt ttgccaggtt
     1681 ccatgacgac caaactgtga gttatcgtca ctgcgtgctg cgccttcaaa ggtaaaatcg
     1741 ccgacttgtt gcagcccggt cagatagatg ccggtattac gttgatcata tccatcctca
     1801 acataacctg tacccggcgt cgtagtctgt ttctgccagt cgacacccgc accaatacta
     1861 ccgtgaccaa cgatgacatt gtttgcccac tggacggtgt attgcttcat ctcatcgagc
     1921 gtcgccgacg aatcataacg accataatgg ggatcgtagt tgtaatcttt gctatggcta
     1981 tagctggtaa tgagttgtga tttaatcagt tcgccgttat agcgcagccc ggcgtcccaa
     2041 ctttggctat agagtttacg ggtatcgagc aacggtgaac cgggagaata atacgcgtca
     2101 taattggtac ggttatcata gccatagccg cgcacaaagc cgctccaggc atcagtaaag
     2161 ttatgctcca gcgcgccata aagcgtttta cttaaaaaac catcgttatc tgtctgcgct
     2221 tgcgttcctg tattaccata ggcaacaaca tcataaccat gagtatgggc ataatcgccc
     2281 aacagcgtta cccgtgtctt atcccccagt tgttgctgcg tagagacatc atagttctga
     2341 taactattgc ttccccaccc tgctgaaatt tccgttccgg gttcatcgcg cgtcgtgatg
     2401 atattcacca ccccgcctat tgcatcggaa ccataaacag cggagcgcgg cccacggata
     2461 tattcaacac gctggacaag cgcaataggg aactggctaa ggtcggcaga accactcacc
     2521 cccgccagat tcaggcgtac gccatcaatt aacaccaaca catgactggc atttgtaccg
     2581 cgaataaaaa tagatgagag ctgacctgaa ccgccgtttt gggtgatatc gacgcccgga
     2641 agacggcgca gcacatcatt gaccgaggtc gactgccagc ggtcgatatc ctgacgggtc
     2701 acaacggtgg ttggtgcaag cacagtgctg cgcggctgtt caaaacggtt agcagtaacg
     2761 acgagagtat ccgggctggt atcctgtgcc caagcggaaa aggctgtgac agaacacgcc
     2821 gtcagcagcg aagctttttt aatcattgta aagcatccac aatagaagaa ggatgccgca
     2881 ggtttcatca atattacgcg atgatgagaa ccagatgcga cgttggccgg caggtcttcg
     2941 ggcttggagg ggtatctaag atactaagag atgatgactt cccaccggat ggcagtgtcc
     3001 gcataacgca atcatcgcac ctttccttac cgctgcgcgt cagctccaga ttcgcactgg
     3061 attccctatt aactcacagg accgacaagt ggatgctaca ggttgtaaca agttactgtc
     3121 cagacgtagc tcacaaatag gaattcatca agatctggac atctgatgag caatccctac
     3181 aatcgccgcg tactttaatt tttcaggata catcatgacc cccgaacacc ttccaacaga
     3241 acagtatgaa gcgcagttag ccgaaaaagt ggtacgtttg caaagtatga tggcaccgtt
     3301 ttctgacctg gttccggaag tgtttcgctc gccggtcagt cattaccgga tgcgtgcgga
     3361 gttccgcatc tggcacgatg gcgatgacct gtatcacatc attttcgatc aacaaaccaa
     3421 aagccgtatc cgcgtggata gcttccccgc cgccagtgaa cttatcaacc agttgatgac
     3481 ggcgatgatt gcgggtgtgc gtaataatcc cgttctgcgc cacaagttgt tccagattga
     3541 ttacctcacc acgctgagta atcaggcggt ggtttccctg ctataccata agaagctgga
     3601 tgatgagtgg cgtcaggaag cggaggccct gcgcgatgca ctgcgcgcgc agaatctgaa
     3661 tgtgcatctg attggtcggg caacgaaaac caaaatcgag ctggatcaag attacatcga
     3721 cgagcgtctg ccggtcgcag ggaaagagat gatctaccgt caggtagaaa acagctttac
     3781 ccagccgaac gcggcgatga atattcagat gctggaatgg gcgctggacg taaccaaagg
     3841 ctcaaaaggc gatttactgg agctgtactg cggcaacggt aacttttcat tagcgctggc
     3901 gcgcaatttt gatcgggtat tagccaccga aatcgctaag ccgtcggttg ctgctgcgca
     3961 atacaacatc gcagctaacc atattgataa cgtacaaatt attcgtatgg cggcagaaga
     4021 atttactcag gcgatgaatg gtgtgcgcga gtttaaccgc ctgcaaggga tcgacttaaa
     4081 gagttatcag tgcgaaacca tttttgtcga cccaccgcgc agcggtctgg acagtgaaac
     4141 cgagaaaatg gtgcaggcgt atccgtgtat tttgtacatc tcctgtaacc cggaaacgtt
     4201 atgcaagaat ctggaaacat taagccagac gcacaaggtc gaacgtctgg ctctgtttga
     4261 tcagttcccc tacacgcacc atatggagtg cggcgtatta ctgaccgcga agtaaaaccc
     4321 atgccggatg cgccagcatc cggcataata ccgattactc agcaacttcc tgcttacggt
     4381 tacgcatctt cgcgccaatc cagaacacca taatgacgga gagtactgcc gggaagaagt
     4441 tagagccgat atccggatat tccgcacgaa ctaccgtgct gtacagcagt acgccgagaa
     4501 taaaacaggc agctgccaga cccggcaagc ctaccggcat agtgcgatta agataacgtt
     4561 gatgcaggca gtaaaccgtc agcaccaggg aaataatcgg gaatacagaa aatggcacaa
     4621 tggagctaaa cagcgctgcg aaagtaccat taatcgataa gccagcaact aacgccagca
     4681 gcagcgtacc tctatcttga tttgcttgtt tcattactcg tccttcacat ttcccggaat
     4741 aattgcggtt ttctcttgtt cacggcgata ccagtaataa gcccctttcg aaatcattcg
     4801 cagttgcagt accagtcgct cttctaattg ccgacgttgt tcgacgccga cgtccaacgc
     4861 ctcggcaccc gcactgaaga caattgtcac cattgcttcg gcttgcgctt cagtaaacgc
     4921 acgcggcata tggttttcga gttccagata gtccgcaagt tccgcaatga agtgctgaat
     4981 ttcacgcgca acggcggcac gaaacgcagc ggaggtgccg gagcgttccc gcaataataa
     5041 ccggaaggcg ttaggattat taccgatgaa ctccataaat gtggagaccg aggtgcggat
     5101 cacactcccg cctttggcga tacgctgacg cgcctggcgc atgagttggc gtagcattaa
     5161 accgctctca tcaaccatgg tcagacccag ttcgtctacg tcgcggaaat gccgataaaa
     5221 agaggtggga gcaatgcccg cttcacgcgc cacttcacgc aaactcaggc tggcgaagct
     5281 gcgttcagca cttaattggc taaatgcggc ttccaccagc gaacggcggg ttttttcttt
     5341 ttgttgcgct cttatgccca tcacgatgtc tgaatccttg ccaaaagtac tgctggcact
     5401 ataccagaga atgaacatac tggattgctc cagtattgtg aatgaacggt aacgcaataa
     5461 aacatattta ctggccggtt cttttacgta cagcggaaac ctgccgctta aacggagagt
     5521 atcgtcgata aaaatccaat aaaacgtcag ggcaaaagta agaaacagac aaagcaaagg
     5581 ccgctcagga tatagccaga taaatgacgg ggatcaattg gcttacccgc gatagaatgt
     5641 taccattctg ttgcttttat gtataagaac aggtaagccc taccatgcca cattcctacg
     5701 attacgatgc catagtaata ggttccggcc ccggcggcga aggcgctgca atgggcctgg
     5761 ttaagcaagg tgtgcgcgtc gcagttatcg agcgttatca aaatgttggc ggcggttgca
     5821 cccactgggg caccatcccg tcgaaagctc tccgtcacgc cgtcagccgc attatagaat
     5881 tcaatcaaaa cccactatac agcgaccatt cgcgactgct ccgctcttct tttgccgata
     5941 tccttaacca tgccgataac gtgattaatc aacaaacgcg catgcgtcag ggattttacg
     6001 aacgtaatca ctgtgaaata ttgcagggga acgctcgctt tgttgacgag catacgttgg
     6061 cgctggattg cccggacggc agcgtcgaaa cactaaccgc tgaaaaattt gttattgcct
     6121 gcggctctcg tccatatcat ccaacagatg ttgatttcac ccatccacgc atttacgaca
     6181 gcgactcaat tcttagcatg caccacgaac cgcgccatgt acttatctat ggtgctggag
     6241 tgatcggctg tgaatatgcg tcgatcttcc gcggtatgga tgtaaaagtg gatctgatca
     6301 acacccgcga tcgcctgctg gcatttctcg atcaagagat gtcagattct ctctcctatc
     6361 acttctggaa cagtggcgta gtgattcgtc acaacgaaga gtacgagaag atcgaaggct
     6421 gtgacgacgg tgtgatcatg catctgaagt cgggtaaaaa actgaaagct gactgcctgc
     6481 tctatgccaa cggtcgtacc ggtaataccg attcgctggc gttacagaac attgggctgg
     6541 aaactgacag tcgcggacag ctgaaggtca acagcatgta tcagaccgca cagccgcacg
     6601 tttacgcggt gggcgacgtg attggttatc cgagcctggc atcagcggcc tatgaccagg
     6661 ggcgcattgc cgcgcaggcg ctggtaaaag gcgaagccac cgcgcatctg attgaagata
     6721 tccctaccgg catttacacc atcccggaaa tcagctctgt gggcaaaacc gaacagcagc
     6781 tgaccgcgat gaaagtgcca tatgaagtgg gccgcgccca gtttaaacat ctggcacgcg
     6841 cacaaatcgt cggcatgaac gtgggcacgc tgaaaatttt gttccatcgg gaaacaaaag
     6901 agattctggg tattcactgc tttggcgagc gcgctgccga aattattcat atcggtcagg
     6961 cgattatgga acagaaaggt ggcggcaaca ctattgagta cttcgtcaac accaccttta
     7021 actacccgac catggcggaa gcctatcggg tagctgcgtt aaacggttta aaccgcctgt
     7081 tttaacactt tatcgaaata gccatccatt cttgctcgga tggcctctgc cagctgctca
     7141 tagcggctgc gcagcggtga gccaggacga taaaccaggc caatagtgcg gcgtggttcc
     7201 ggcttaatgc acggcagata aacaacccca tcgcgtttgc gctccggcgg cacagccagc
     7261 gctggcagta aagtgatccc gctacctgcc gccaccatgt tgcgcagcgt ctccaggctg
     7321 gtcgcgcgga agtgtgtatc ttcatccgcc ccggcttcaa aacagaaacc cattgcctga
     7381 tcgcgcaaac agtgaccatc ttccagcatc agcagttttt cccctgccag atcggccatc
     7441 ggtacgcatt cgcggttcgc ccacgggtga tcttcataga tagccagcaa cattggctca
     7501 tcaaacaacg gcacttcaat gaatgcttcg ctctctttca ccagcgcgag gatcacgcaa
     7561 tcgagtttgc cactgtccag ttgcgccagt aactggtggg tctgtgcttc atgcagatac
     7621 atttccagct ttggaaaggt ctggtgcagc atcgggataa tatgcggtag caggtacggt
     7681 ccaaccgtgg gaatcaaacc aatatgcagc ggtccggaca tcgtctcgcc ctgctggctt
     7741 gccatctctt taaggacttt cacctcacgc agcacggtac gcgcctgatc caccagcagc
     7801 attcccgcct gggtgaacaa cactttacgg ctggtccgct ccagcaacat cacgcccagc
     7861 tcatcttcca gcttacgaat ttgcccgcta agcgtcggct ggctaacgtg gcaggaatct
     7921 gccgcacgcc gaaaatggcg gtgttcagcc aatgccacca ggtactcaag atcacgaata
     7981 ttcattatcc atcctccatc gccacgatag ttcatggcga taggtagaat agcaatgaac
     8041 gattatccct atcaagcatt ctgactgata attgctcaca gaaacggagc ggcacctctt
     8101 ttaacccttg aagtcactgc ccgtttcgag agtttctcaa ctcgaataac taaagccaac
     8161 gtgaactttt gcggatctcc aggatccgct tttttttgcc ataaaaaagc ccggcgataa
     8221 gccaggctca aatttgtaca tataaatgtt cttaccccaa ccgcgcctgc gcaaaagcaa
     8281 tcgcttgggc cacctgctgc ggtgagacgc cgccttttgc cgcacgcttg tcgaggcacg
     8341 attgcagcga caaaatcgga tagacatctt cgccaatcac cgggctgaat ttctgcaact
     8401 cgtcgagcgg cagatcttcc agcggtttgc cctgacgaat ggcttccacc accgcttcac
     8461 ccacgatatg gtgcgcctcg cggaaaggta cgcctttcgc caccaggtaa tccgccagtt
     8521 cggtggcgtt ggcgtaaccc tgctgagccg cttcctggca acgtggacgt ttcacctgaa
     8581 tgccgtccag caccagcgcc gccatatgca ggcagtccag ccaggtatcg agcgcgtcga
     8641 acagaccttc tttgtcttcc tgcatatctt tgttgtaagc cagcggcaaa cctttcagcg
     8701 tcatcatcat gccggttaac gcaccctgca cccgaccgca tttaccgcga atcagctcca
     8761 gcgcatccgg gtttttcttc tgcggcatta atgatgaacc ggaagtcacg cggtcagaaa
     8821 gctccacaaa ccccgcttcg ccggtgttaa agaaaatcag atcttcagca aaacgcgaca
     8881 gatgcaccat gccgatagcg gcagccgaca gcagttccaa cacatggtca cggtcagaaa
     8941 cgctgtccag actgttacgg gtcgccgaag caaagcccag ccagcctgct aactgttcac
     9001 ggtcgatttc ataggccgtt cccgccagcg cgccacagcc taatgggctg acatccagac
     9061 gcttaagcgc atcctgcaaa cggctttcat cacgcgccag catctcaaca taggccaggc
     9121 accagtgcgc gaacgtcacc ggctgggcgc gttgcaggtg agtgtaacct ggcatgaccg
     9181 cgtcctgatt gttttgtgcg gtttccacca gcgccgattg cagctgtcgg ttagccgtca
     9241 gtaactcgct aacggtatct ttgcaccaca gtttcaggtc agtcgctacc tgatcgttac
     9301 gactacgccc ggtatgcagc tttttgccta actggcccac tttgtcgatc agtttgcctt
     9361 ccacccagct atggatatct tcggcgtcgc tttcaaggat ttgttgtggc ctggcgcgaa
     9421 catcttccag cagcacgttc agcgcctctt ccagttgcgc ctgctcttct gcggttaaca
     9481 cgcctaccgt gaccagggct ttggaccagg ccacagagcc aacaatatcc tgctccgcca
     9541 gacggtaatc aaagcgcagt gagtcgttga attgtttgaa ccgttgatct gctgcctggg
     9601 taaaacgccc gccccaaagt gccataactc tgtttcctta ttttgaaatt caatgccgga
     9661 aagctgcgcc tccggccaac aaaacttaag ctaaaatccg cgtacccatc ggcataccgt
     9721 taaacagtgc cggaagctgc tccgcatgac gccaggaggc gatatctacc ggacggccca
     9781 gcgtgcgggc cgcatccagc gccgcgttca ctttcactat catgccgtca gtaataatgc
     9841 cctgctcaat cagttgttct gctttcgcgg cggtcatttc ggcaatgcgt tgccctttgc
     9901 cgtcgagaat gccgctgacg tcggagagca aaatcagatc cgcgcccagc gttgccgcca
     9961 gcgccgttgc cgcctggtcg gcattgacgt tcatcagttg cccttcgtct gttacgccaa
    10021 tggagctgac caccggcaga taaccgttct ccagcaagga gttgataagc ttaggcgaac
    10081 ctggctgcgc cagtccaaca tgacctaact cttcatcaag ctgggtcact ttgacgctgt
    10141 cgccgtcacc gagaaacaaa cctacggccg caatctgatg tttcttcgcc catgccaaca
    10201 gggttttatt tgccgttccc gccagtgctc cggtgataat gtctatctga tcagcaggcg
    10261 tcacccgcag gccgtttttc tttttcaccg gcagattcag ccctttcatc agctcatcca
    10321 ccacgcaacc gccgccgtgc acaatcacca gcggacgctg atgtgactca cgataattca
    10381 ccagtgtgct aaacagacgt tccagcgcct cttcactatc cagcagtacg ccgcccagtt
    10441 tgataattaa tggattcatc attgcaccct taaataagag actgcgtttc cgcatagccg
    10501 aaacgaatat tggcgcactg taccgcttgt gccgccgcgc ctttcagtaa gttgtcttcg
    10561 gtcgccacaa taatcagatg ctcgccctga acggcaaacc cgatatcgca aaatggcagc
    10621 ccaacgacat ttttcagcgc cggaacgcct ttgtcataca gccgcaccag cggtttatgg
    10681 gcatacgcct gctgtaacac ttgcgcgact tgcgcctggg tcacacccga tttcaggcgg
    10741 caggtaatgg tttcgagaat gccgcgcggg aaattgccca gatgtggggt gaagataacg
    10801 tcagcaccga ggtgtgtggc gatctctggt tgatggcgat gagtaaagac gccatacggt
    10861 tgcaggctaa cttcacaaaa actatttgaa atcgctgcct tacgtcctgc accgctcacg
    10921 ccgctggtgg cgttgatcac cggccactga ttgaggtcaa gaagatcggc atcaatcaac
    10981 ggtttcagcg ccagctgtgc cgccgtcgga taacagcctg gcaccgcaat caaattcgct
    11041 tcttttaatt tattaccgca ccactccgcc agaccgtagg ctgcctgttc cagcagttcc
    11101 gggtattgat gggtaaagcc gtaatatttt tcatagaagg tggcgtcgtt aacacgaaac
    11161 gcaccggaaa ggtcgaacac cacgcagccc gcttcaagaa actgcggcgc taaatcgtgg
    11221 ctaacttcat gggcggtggc gagaaacact acgtccaccc ctgggctaaa ctcgctgata
    11281 tccgacatcg gctgcaacgg cagctcaacg atgcctttta gctgcggatg caaatcggag
    11341 attaactttc ccgcatcatt gctttgcgct gaaacagtca aagcggttat gttcatatgc
    11401 ggatggcgat ttacataggt cactagctct gcgccagcgt agccgctggc acccacaatc
    11461 agcgtattca acatcggggc tattcacctt cttatgtctg gttgccaggt taaacgtaaa
    11521 acattcacct tacggctggt gggttttatt acgctcaacg ttagtgtatt tttattcata
    11581 aatactgcat gaatattgat actatcatga ccagaggtgt gtcaacaatg aaaaacaaat
    11641 taccgccatt tatcgagatt taccgtgctc tgattgccac accttcaata agcgccacgg
    11701 aagaggcact cgatcaaagc aatgcagatt taatcactct gctggcggac tggtttaaag
    11761 atttaggctt caatgtggaa gtgcagcctg tgccaggaac tcgcaacaaa ttcaatatgc
    11821 tggcaagtat cggacagggg gctggcggct tgttgctggc ggggcatacc gatacggtgc
    11881 catttgatga cggtcgctgg acgcgcgatc cgtttacact gacggagcat gacggcaagc
    11941 tttacggctt aggcaccgcc gacatgaaag gcttttttgc gtttatcctt gatacgctac
    12001 gcgatgtcga cgtcacgaaa ctgaaaaaac cgctctacat tctggcgact gctgatgaag
    12061 aaaccagtat ggccggagcg cgttattttg ccgaaactac cgccctgcgc ccggattgcg
    12121 ccatcattgg cgaaccgacg tcactacaac cggtacgcgc acataaaggt catatctcta
    12181 acgccatccg tattcagggc cagtcggggc actccagcga tccagcacgc ggagttaacg
    12241 ctatcgaact aatgcacgac gccatcgggc atattttgca attgcgcgat aacctgaaag
    12301 aacgttatca ctacgaagca tttaccgtgc cataccctac gctcaacctc gggcatattc
    12361 acggtggcga cgcttctaac cgtatttgcg cttgctgtga gttgcatatg gatattcgtc
    12421 cgctgcctgg catgacactc aatgaactta atggtttgct caacgatgca ttggctccgg
    12481 tgagcgaacg ctggccgggt cgtctgacgg tcgacgagct gcatccgccg atccctggct
    12541 atgaatgccc accgaatcat caactggttg aagtggttga gaaattgctc ggagcaaaaa
    12601 ccgaagtggt gaattactgt accgaagcgc cgtttattca aacgttatgc ccgacgctgg
    12661 tgctcggtcc gggttccatc aatcaagctc atcagcctga tgaatatctg gagacgcggt
    12721 ttattaaacc cacccgtgag ttgattatcc aggtaattca ccatttttgc tggcattaaa
    12781 acgtaggccg gataaggcgc tcgcgccgca tccggcaccg ttgccaaact ccggtgccgc
    12841 aataatgtcg gatgcgatgc ttgcgcatct tatccgacct acacctttgg tgttatgtat
    12901 ggcgattttt taacatttcc ataagttacg cttatttaaa gcgtcgtgaa tttaatggcg
    12961 taaattcctg ctatttattc gtttgctgaa gcgatttcgc agcatttgac gtcaccgctt
    13021 ttacgtggct ttataaaaga cgacgaaaag caaagcccga gcatattcgc gccaatgcga
    13081 cgtgaaggat acagggctat caaacgataa gatggggtgt ctggggtaat atgaacgaac
    13141 aatattccgc attgcgtagt aatgtcagta tgctcggcaa agtgctggga gaaaccatca
    13201 aggatgcgtt gggagaacac attcttgaac gcgtagaaac tatccgtaag ttgtcgaaat
    13261 cttcacgcgc tggcaatgat gctaaccgcc aggagttgct caccacctta caaaatttgt
    13321 cgaacgacga gctgctgccc gttgcgcgtg cgtttagtca gttcctgaac ctagccaaca
    13381 ccgccgagca ataccacagc atttcgccga aaggcgaagc tgccagcaac ccggaagtga
    13441 tcgcccgcac cctgcgcaaa ctgaaaaacc agccggaact gagcgaagac accatcaaaa
    13501 aagcagtgga atcgctgtcg ctggaactgg tcctcacggc tcacccaacc gaaattaccc
    13561 gtcgtacact gatccacaaa atggtggaag tgaacgcctg cttaaaacag ctcgataaca
    13621 aagatatcgc tgactacgaa cacaaccagc tgatgcgtcg cctgcgccag ttaatcgccc
    13681 agtcatggca tactgatgaa atccgtaagc tgcgtccaag cccggtagat gaagccaaat
    13741 ggggctttgc cgtagtagaa aacagcctgt ggcaaggcgt accgaattac ttgcgcgaac
    13801 tgaacgaaca actggaagag aacctcggct acaaactgcc cgtcgaattt gttccggtcc
    13861 gttttacttc gtggatgggc ggtgaccgcg acggcaaccc gaacgtcact gccgatatca
    13921 cccgccacgt cctgctactc agtcgctgga aagccaccga tctgttcctg aaagatattc
    13981 aggttctggt ttctgaactg tcgatggttg aagcgacccc tgaactgctg gcgctggttg
    14041 gcgaagaagg tgccgcagaa ccgtatcgct atctgatgaa aaacctgcgt tctcgcctga
    14101 tggcgacaca ggcatggctg gaagcgcgcc tgaaaggcga agaactgcca aaaccagaag
    14161 ggctgctgac acaaaacgaa gaactgtggg aaccgctcta cgcttgctac cagtcacttc
    14221 aggcgtgtgg catgggtatt atcgccaacg gcgatctgct cgacaccctg cgccgcgtga
    14281 aatgtttcgg cgtaccgctg gtccgtattg atatccgtca ggagagcacc cgccataccg
    14341 aagcgctggg cgagctgacc cgctacctcg gtatcggcga ctacgaaagc tggtcagagg
    14401 ccgacaaaca ggcgttcctg atccgcgaac tgaactccaa acgtccgctt ctgccgcgca
    14461 actggcaacc aagcgccgaa acgcgcgaag tgctcgatac ctgccaggtg attgccgaag
    14521 cgccgcaagg ctccattgcc gcctacgtga tctcgatggc gaaaacgccg tccgacgtac
    14581 tggctgtcca cctgctgctg aaagaagcgg gtatcgggtt tgcgatgccg gttgctccgc
    14641 tgtttgaaac cctcgatgac ctgaacaacg ccaacgatgt catgacccag ctgctcaata
    14701 tcgactggta tcgcggcctg attcagggca aacagatggt gatgattggc tattccgact
    14761 cagcaaaaga tgcgggcgtg atggcagctt cctgggcgca atatcaggca caggatgcat
    14821 taatcaaaac ctgcgaaaaa gcgggtattg agctgacgtt gttccacggt cgcggcggtt
    14881 ccattggtcg cggcggcgca cctgcccatg cggcgctgct gtcacaaccg ccaggaagcc
    14941 tgaaaggtgg cctgcgcgtg actgaacagg gcgagatgat ccgctttaaa tatggtctgc
    15001 cagaaatcac cgtcagcagc ctgtcgcttt acaccggggc gattctggaa gccaacctgc
    15061 tgccaccacc tgagccgaaa gagagctggc gtcgcattat ggatgaactg tcagtcatct
    15121 cctgcgatct ctaccgtggc tacgtacgtg aaaacaaaga ttttgtgcct tacttccgct
    15181 ccgctacgcc ggaacaagaa ttgggcaaac tgccgttggg ttcacgtccg gcgaaacgtc
    15241 gcccaaccgg cggcgtcgag tcgctgcgcg ccataccgtg gatttttgcc tggacgcaaa
    15301 accgcctgat gctccccgcc tggctgggtg caggtacggc gctgcaaaaa gtggtcgaag
    15361 atggcaaaca gagcgagctg gaagccatgt gccgcgattg gccattcttc tcgacgcgtc
    15421 tcggcatgct ggagatggtc ttcgccaaag cagacctgtg gttggcggaa tactatgatc
    15481 aacgtctggt agacaaagca ctgtggccgt taggtaaaga gttacgcaac ctgcaagaag
    15541 aagacatcaa agtggtgctg gcgattgcca acgattccca tctgatggcc gatctgccgt
    15601 ggattgcaga gtctattcag ctacggaata tttacaccga cccgctgaac gtattgcagg
    15661 ccgagttgct gcaccgctcc cgccaggcag aaaaagaagg ccaggaaccg gatcctcgcg
    15721 tcgaacaagc gttaatggtc actattgccg ggattgcggc aggtatgcgt aataccggct
    15781 aatcttcctc ttctgcaaac cctcgtgctt ttgcgcgagg gttttctgaa atacttctgt
    15841 tctaacaccc tcgttttcaa tatatttctg tctgcatttt attcaaattc tgaatatacc
    15901 ttcagatatc cttaaggaat tgtcgttaca ttcggcgata tttttttcaa gacaggttct
    15961 tactatgcat tccacagaag tccaggctaa accccttttt agctggaaag ccctgggttg
    16021 ggcactgctc tacttttggt ttttctctac tctgctacag gccattattt acatcagtgg
    16081 ttatagtggc accaacggca ttcgcgactc gctgttattc agctcgctgt ggttgatccc
    16141 ggtattcctc tttccgaagc ggattaaaat tattgccgca gtaatcggcg tggtgctatg
    16201 ggcggcctct ctggcggcgc tatgctacta cgtcatctac gggcaggagt tctcgcagag
    16261 cgttctgttt gtgatgttcg aaaccaacac caacgaagcc agcgagtatt taagccagta
    16321 tttcagcctg aaaattgtgc ttatcgcgct ggcctatacg gcggtggcag ttctgctgtg
    16381 gacacgcctg cgcccggtct atattccaaa gccgtggcgt tatgttgtct cttttgccct
    16441 gctttatggc ttgattctgc atccgatcgc catgaatacg tttatcaaaa acaagccgtt
    16501 tgagaaaacg ttggataacc tggcctcgcg tatggagcct gccgcaccgt ggcaattcct
    16561 gaccggctat tatcagtatc gtcagcaact aaactcgcta acaaagttac tgaatgaaaa
    16621 taatgccttg ccgccactgg ctaatttcaa agatgaatcg ggtaacgaac cgcgcacttt
    16681 agtgctggtg attggcgagt cgacccagcg cggacgcatg agtctgtacg gttatccgcg
    16741 tgaaaccacg ccggagctgg atgcgctgca taaaaccgat ccgaatctga ccgtgtttaa
    16801 taacgtagtt acgtctcgtc cgtacaccat tgaaatcctg caacaggcgc tgacctttgc
    16861 caatgaaaag aacccggatc tgtatctgac gcagccgtcg ctgatgaaca tgatgaaaca
    16921 ggcgggttat aaaaccttct ggatcaccaa ccagcagacg atgaccgccc gcaataccat
    16981 gctgacggta ttttcgcgcc agaccgacaa gcagtactac atgaaccagc aacgtacgca
    17041 gagtgcgcgt gaatacgaca ccaacgtgct gaagccgttc caggatgtgc tgaatgaccc
    17101 tgcgccgaag aaactgatca tcgttcatct gctgggtacg catatcaaat acaaataccg
    17161 ctacccggaa aatcagggca agtttgatgg caataccgat catgttccgc caggattaag
    17221 cgcagaagag ctggaatcat ataacgatta tgacaacgct aacctgtata acgatcatgt
    17281 ggttgccagc ctgattaaag actttaaagc ggcagacccg aacggattcc tggtttactt
    17341 ctctgaccac ggtgaagagg tttacgacac gccgccgcac aaaactcagg ggcgtaacga
    17401 agacaacccg acgcgccaca tgtacaccat tccgttcctg ctgtggacgt cggaaaaatg
    17461 gcaagcgact catccccgtg atttctcaca ggatgtcgat cgtaaataca gcctggcgga
    17521 actgatccac acctggtcag atttggcggg cttatcttac gacggttacg atccaacccg
    17581 ttcagtggtg aatccgcagt tcaaagaaac tacccgctgg attggtaacc cgtacaagaa
    17641 aaacgcgctg atcgattacg acactctgcc gtatggcgac caggtaggta atcagtaatt
    17701 atcagccagg cgctatttgc cggatgcgac gcttgacgcg tcttatccgg cctacagatc
    17761 gcggcggtag gctggataag gcgtttacgc cacatccggc aatacaactg ccgcaatgtt
    17821 tacatcgaac tcccctcagg ccacactttt caggtggcct tttttaataa tgccagttac
    17881 tgttttcccg tctacatacc taagggaata acatgtatca cgacgtcagc tatctgctct
    17941 cccgcctgat caacggcccg ctgtccctgc gccagattta ctttgccagc agtaacggcc
    18001 cggtgcccga tcttgcgtat caggtcgatt ttccacggct ggaaattgtg ctggaaggtg
    18061 agtttgttga taccggcgct ggagcaacgt tagttcccgg cgatgtgctg tacgttgccg
    18121 ctggtggctg gaatttccca caatggaaaa cacccgctac cacctttagc gtgctgtttg
    18181 gcaaacagca actcggcttc agcgtcgtgc aatgggatgg caaacaatat caaaatctgg
    18241 cgaagcaaca cgtcgcccga cgcggcccac gtataggttc ttttctgcta caaacgctca
    18301 atgaaatgca aatgcagccg caggagcagc aaacggcaag gcttatcgta gccagcctgc
    18361 ttagccactg ccgcgatttg ctcggcagcc aaatccagac cgcctcacgc agccaggcac
    18421 tatttgaagc tattcgcgat tatatcgacg aacgctatgc ctccgcgctt acccgcgaat
    18481 ctgtcgcaca ggcattctat atttcaccaa actatctctc gcacctgttt caaaaaacgg
    18541 gggccattgg ttttaacgaa tacctgaatc acacgcgact ggagcacgct aagacgttat
    18601 tgaaaggcta tgacctgaag gtaaaagaag tggcgcacgc ctgcggcttt gtcgacagca
    18661 actacttctg ccgtctgttt cgtaaaaaca ccgaacgctc gccgtcggaa tatcgccggc
    18721 agtaccacag ccaactgaca gaaaaaccga ctactccaga ataagatggg tttgctgcgg
    18781 cgcagaaagc actttgcgca ccgcgctcat tacccgctgc ggctcacgca ggaatgcgta
    18841 gatgctgcat tgcacatagc ggcaatgttc aaatcgctcg gcaccggcaa gctcgatatc
    18901 cgtaatcaac agagcaacat ccgcccgacg gatatcctcg tccgctaaac gattctccgt
    18961 tcccagcgcg ccctgagttt caatgctgac gccccacttc tctaactggc acaacttttc
    19021 cagccgttcc gccgccatat aagtatgcgc cacgccgctt acgcaggcgg ttactgccac
    19081 caggtatgcc attttaacct cccacggtaa cctgaaatcc ggcccgttct gccatttcgc
    19141 gcatcgttgt cacatcggct gacgacggcg caggcacctc tttcatcgac catgttttcc
    19201 ccagcaggcg gtatttcggt tcgccgtact gatgaaacgg taacagatgg atctgcctga
    19261 ttttcagcgg gatcagtaca tccagcgcct gctgcatatt ctccctgctg agcgtgaaac
    19321 cagggatcaa cggtaaacgc gggatcacgt tgacaccctc actcaccagc aaacgcagat
    19381 tctccagcac gcgtggcagg ttcatcttca ccacatcccg cgcctgagtc gcgtccataa
    19441 tttttaaatc gaacagcact tcatcgcaca atttcgccag cggtaacagc ttgcttgctg
    19501 gcgcgtctcc ggcagtttca atggcgcatg acacacccca cagccgcagt cgctgtaaaa
    19561 aacgggtagc aaactccgcc tgcattaaca cttcgccgcc agaaagcgtg acgccgccgc
    19621 cggacgtgcg aaagaaaatg tcatctttca tcacttcccg ttccagagcg tcaaggctga
    19681 tatcgcgacc aatccgttca aacgccccgg aggggcattc atccgcatca cgcaaacatt
    19741 tcgcacagtg cagacatttc gcctctctgc gtaccgtctg gattttgccg gagatcgact
    19801 ccggattagc acaccacggg caaagatgcg gacagccttt aaaaaagacc accgtacgaa
    19861 tgccctcgcc gtcattcagt gaataacgct gaatgttgaa aatgcgcgcc acatcatcgc
    19921 ggcgcgtttc cacaacgtta cagctgatgc gctgtccggc ggatgatgtc atcctggatc
    19981 tccttcgaca gttcgacaaa gaaggcgctg tatccggcaa cgcgcaccac cagcccggca
    20041 taatcttgtg ggcgctgttg cgcttcccgc aacgtgtcgg cgttcaccac gttaaactga
    20101 atatgctgca acttaagctg ggtaaacgcc cgtaagaagt cggccagttt gcgtaatccc
    20161 gcttcacctt ccagggtcgc cggagtgaat ttcacgttca gcaacgtacc gttagacagc
    20221 agcgtattat cgagcttact gactgacttc agtaccgctg ttggcccttg tgcgtcctgg
    20281 cccagcatag gtgacaagcc gccgtctgcc agttgttccc cggcaaaacg accgtctggc
    20341 gtcgcgccaa ccaccgatcc caacgggacg tgagcagaaa cggtatacga tcccggcgtg
    20401 aagtaaccgc gcgcgggttc tggtattttt ccacttcttt gcagtagtgg cgtaacagtt
    20461 cggcgctgat gttatccacc tcgtcgatat cgttaccgta tttctcgaag cggttaatta
    20521 agcgagcacg gactttttcg ccttctggcg ttgcaaagtt ggcttttaat accgacagca
    20581 attcgtcaaa acttaaacgc tgttgctcaa aaaccatccc tttcaacgca tggagagaat
    20641 cgctcaggtt ggcgataccg atcccctgta cgccggagaa gttataacgc gcgccgccat
    20701 cggtaatatc gcgacctttt tccagacaat cgctgataaa agacgagagc agcggtacag
    20761 gtgcccagtc gcgatggccg atatcacaaa tattgctgcc ctcaaccatc agggtgatgt
    20821 agtggctgat cttggcgcgg atctgctcca gtaaaccttc ataagtcagc gcagcattgc
    20881 cttcattttc atgcaggcag atttccatca ctttcagcag attaaacatc gcgatgtcat
    20941 gcaagccgta ggttctgccg ggaatagata attccacaca gcccactacg gaatagtcgc
    21001 gcgcatcttc cagcgaaacg ccacggttga ggaacgctgg taccaccact tcatcgttaa
    21061 agatttgcgg aataccggtg ccgaggcgaa tggtttcggc ggttttcatc aggaacggcg
    21121 tgtcgatcaa ggcgttagtg cgcacgccga ggttcggttg cggtaattgc acgctttgat
    21181 aggcgtcaag gcaaaggaac gaaagcacgt tcaccgcgct acgtccgttc tcggttaacc
    21241 cgccgagcag tgcggtatag ccggtcggga aacctgcgaa ataacgcgcg ctgctggtgg
    21301 agcgcaacag cacgatgtcg ttgcatttca cccataaaga ttcgagcagt tctttcagga
    21361 acgccggatc ttcgccctgg gttaaagatg cctgatagaa cggcaacata tactggtcga
    21421 agcgccctaa agacagcgaa ctggcgttgg attcgtattg cagaatgatg ttcatgtacc
    21481 agaataactg gcacgcctgc cagaacgtct gcggcttatg ttcggcgtta tggcgagaga
    21541 tctccgcaat agtcagcagt tcttcgcgac gctgtgcatc tgtgcagttt gccgccatcg
    21601 tttccgccag ttcggcgtaa cgcaaaatgt gtttctgcga ggcttccagc agtaacaacg
    21661 cggcctgata aaagtgattc tccggctgtt gctgacaatg ttgctgcatc tgtgccacca
    21721 gctcacccag accgtgattc aacaggcgtg ggtaatcaat aataatgtgc ccctggcctt
    21781 tatccgtctg gttgatgctg aaaatctgcg tgttggtcgc ggcttttact tcatctgtca
    21841 tctgcccgtt gatgaaatct ttcatcgaac gtttttccca gtacgggaac aactcttcac
    21901 ggtagatacg tttatcttct tcgctgatag caaagcggtc ctgcggacgc gtcgggaatt
    21961 gatccagctc tttcagcagc cagtaagggt ccatttccgg cgacataatc ccggcgcgcg
    22021 gttttacggt gcggttaccg gcaatcagtt cttcatcacg aatcgaaatt tcaacatgtt
    22081 caaggatata cgctgttgct ttcgcccggc gcaatatcac cggttcgcct tcggtttgcc
    22141 gatggctggc ggtataaagc agcgcccgct ccagcgagat ttcacgggta ttggcaaaca
    22201 gtgcagtttt gaggcgagag atacgattcg tcatgaggaa ctcctgtggg aaagcgaacc
    22261 cgcccttgcg agcgggagaa gaacttaagc ggtttgcgcc aggtgagctt caattttgct
    22321 cataatggcg tcagcacgtt ttactgcatc gctgatgttg acgcgcacga tggtcttgcc
    22381 agcaaaacgc tcttcaaact tgatgccgat atctttggtc agaatcacca tatcagcgct
    22441 cgccacatct tccgcagtca gttcattttc cagaccaatt gacccctagg tttctacttt
    22501 cacttcccag cctttcgctt tcgcggcact ttccagcgcc tctgctgcca tataggtatg
    22561 ggcaacacct gaaggacatg cggttactgc aataattttc gtcatggctc gttcctcaat
    22621 taattaattt caaaatccaa atccaggtcg tcttcttttt catcagtcga agacccattt
    22681 ttacgcgcca gacttttcag cacgttcaca caaacagccg tcaccactgc tccgacagcc
    22741 actgctgcga tataacccag cttgccttca accaccggca gcacaatcag accaccccag
    22801 cctgcatagc actgcgcacc gaccagcgcc gcagttactg caccacaaac tgaaccgacc
    22861 atgatggaag gaatgacacg cagcggatcg gcagcggcga aaggaatcgc cccttcagta
    22921 acgccaacac agcccatcac cagtgccgct ttgccggttt cgcgctcttc tgcggagaaa
    22981 tttttacgac caatcagcgt cgccagcccc atccccagcg gcgggataca aatacccacg
    23041 gcagcgatgg ccaccacggt ataaacaccc tgagcaacgc aaatcagcat gaaggcatag
    23101 gccactttgt taaccggacc gcccatatcg aacgccagca tcagacccat gatcaccgcc
    23161 agcataacaa tgctgccctg ctgcatcccc tgaagccact gagtcaggct gttggtcaac
    23221 gcccctacag gctcgcccaa gccccacatc atgatgcctg cggtaatcag tgtgccgacg
    23281 ataggaataa tgaagatagg catcaccgag cgcagaactt tatgcaccgg aattttcttc
    23341 aggtaatgca ccacgatgcc gccgataatc ccggcgatca gtgcaccaaa gaagcccgca
    23401 ccaaagctgt taccaaccca ggtaccgata gcgcatggag ccagcgcaga acgttctgca
    23461 atggaataac caatgtaagc ggcgaggaaa ggcaccatca gcgtcaagcc cgcaacgccg
    23521 atatcaaaca gttttttcag atttggatcg gctacggcat ccggcactgc gcctttgcca
    23581 tacaacatga cggaaaccgc cagcaaaata ccgcccgata ccacgaaggg aatcatgtgt
    23641 gaaacgcccg tcattaaatg ctgacgggta tttttcagga tctgcaccaa ctcattcata
    23701 agctgctccc gggcttgcga aagcccatgt ccatattttt gatggaggaa acaataggca
    23761 atcgcaggcg gagcaaacag tggataaaaa ggccatttac tggacttttg taatatcagt
    23821 acaaaaatgc gatccgcctc ataacttgcg ataaaccgga agcatgccgc aaaacggcgt
    23881 cttgtgagac gcgtcgcaaa ttgacggtta cattacattt gtccagcttt tggcagattt
    23941 gtcacatggc aggccgttgc cgtgcgctct tatcctgtaa acagaaaacc gtaccgggag
    24001 agaggcaatg gccctgattg tggaatttat ttgtgagcta cctaacggcg tacatgcgcg
    24061 tccggcaagc cacgttgaaa cgctgtgtaa tactttttca tcacaaattg agtggcataa
    24121 cctgcacact gaccgcaagg gcaacgccaa aagcgccctt gcgctgattg gcaccgatac
    24181 gctggcgggc gataactgcc agttactgat ttccggggcc gacgaacagg aagcgcacca
    24241 gcgtttaagc caatggctgc gcgatgaatt cccccactgc gatgcgccgc tggcggaagt
    24301 taaatctgac gaactggaac cactgccggt ttcactgacc aatctgaatc cgcaaattat
    24361 ccgcgcccgc accgtgtgca gcggtagtgc aggcggcatt ctgacgccga tctcttcttt
    24421 agatctcaat gcgctgagta atcttcccgc agccaaagac gttgacgccg agcaatccac
    24481 actggaaaac ggcctgacgc tggtactgaa aaacattgag tttcgtctgc tggatagcga
    24541 cggtgctacc agcgcgatac tggaagctca ccgatccctg gctggcgata catctttacg
    24601 tcagcactta ctggcaggcg tcagcgaggg attaagctgc gccgaagcga ttgtcgccag
    24661 tgcgaatcac ttttgcgaag agttcgcccg ttccagcagc agctacctgc aagaacgtgc
    24721 cctggacgta cgcgacgtct gcttccagtt actccagcaa atctacggtg agcaacgctt
    24781 cccggcaccg ggcaaactga cgcagtccgc catttgtatg gctgatgaac tgacccccag
    24841 ccagttcctc gaactggata aaaatcacct caaaggtctg ttgctgaaaa gtggcggcac
    24901 cacctcacat acggtgatcc ttgcccgttc gttcaacatt ccgacgctgg ttggtgtgga
    24961 tattgatgcc ctcactccgt ggcagcatca aacgatttat atcgacggca acgccggggc
    25021 gattgtggtt gagccaggag aagccgtagc tcgttattat cagcaagaag cccgcgtaca
    25081 ggacgccctg cgtgagcaac agcgtgtctg gctgacccaa caagcccgta ccgctgacgg
    25141 tatccgcatt gaaattgccg ctaacatcgc tcactccgtg gaagcgcagg ccgcattcgg
    25201 caatggtgcg gaaggcgttg gtttgttccg cactgaaatg ctctatatgg atcgcaccag
    25261 tgcaccgggc gaaagcgagc tgtacaacat tttttgtcag gcgctggagt ctgccaacgg
    25321 acgcagcatt attgtgcgca ctatggacat tggcggcgac aaacccgttg attatctaaa
    25381 cattcccgca gaggcaaacc cgttcctcgg ttatcgcgcc gtgcgtattt atgaagaata
    25441 cgcgtcgtta tttaccacac aactacgctc gatcctgcgc gcctccgctc acggcagcct
    25501 gaaaatcatg atcccgatga tctcctcaat ggaagagatc ttatgggtga aagaaaaact
    25561 ggcagaagcc aaacagcaac tacgtaacga acacattccg tttgatgaga agatccagct
    25621 cggcattatg ctggaagtgc cgtcggtgat gttcatcatc gatcaatgct gcgaagagat
    25681 tgatttcttt agtattggta gtaatgacct gacgcaatat ctgctggcag tggatcgcga
    25741 taacgctaag gttactcgtc actacaacag cctgaatccg gcattcttgc gggcgctcga
    25801 ttacgccgtg caggcggtgc atcgccaggg taaatggatt ggtctgtgcg gtgagctggg
    25861 agcgaaaggt tccgtgctgc cgttgctggt cggcttaggg ctggatgaac tcagcatgag
    25921 cgcaccatca attccggcgg cgaaagcgcg gatggcgcaa cttgatagcc gtgagtgccg
    25981 caagttgctc aaccaggcaa tggcctgccg tacttcgctg gaagtggagc acctgctggc
    26041 gcaattccgc atgacccaac aagacgcacc gctggtcacc gccgagtgca tcacactgga
    26101 aagcgactgg cgcagcaaag aagaagtgct caaaggcatg accgataacc tgctgctggc
    26161 agggcgctgc cgctatccgc gtaaactgga agccgacttg tgggcgcgcg aggccgtttt
    26221 ctctaccggt ctgggcttta gttttgccat tccacacagc aaatcagaac acattgagca
    26281 atccaccatc agcgttgcgc gtctgcaagc gccggtgcgc tggggtgatg atgaagcgca
    26341 attcatcatc atgttaaccc tgaacaaaca cgctgcgggc gatcagcaca tgcgcatttt
    26401 ctcgcgcctc gctcgccgca tcatgcacga agaattccgt aacgcgctgg ttaacgccgc
    26461 ctctgccgac gctatcgcca gcctgctgca acatgaactg gaactgtaaa aggaaacatc
    26521 atggaactgt atctggacac cgctaacgtc gcagaagtcg aacgtctggc acgcatattc
    26581 cccattgccg gggtgacaac taacccgagc attatcgctg ccagcaagga gtccatatgg
    26641 gaagtgctgc cgcgtctgca aaaagcgatt ggtgatgagg gcattctgtt tgctcagacc
    26701 atgagccgcg acgcgcaggg gatggtggaa gaagcgaagc gcctgcgcga cgctattccg
    26761 ggtattgtgg tgaaaatccc ggtgacttcc gaaggtctgg cagcaattaa aatactgaaa
    26821 aaagagggta ttactacgct tggcaccgca gtgtacagtg ctgcacaagg gttactggcc
    26881 gcgctggcag gggcaaaata cgttgctccg tatgttaacc gcgtagatgc ccagggcgga
    26941 gacggcattc gtacggttca ggagctgcaa gcgctgttag aaatgcacgc gccagaaagc
    27001 atggtgctgg cagccagctt taaaacgccg cgtcaggcgc tggactgttt actggcagga
    27061 tgtgaatcca tcaccctgcc cttagatgta gcgcaacaaa tgctcaacac ccctgcggta
    27121 gagtcagcta tagagaagtt cgaacacgac tggaatgccg catttggcac tactcatctc
    27181 taaaggagca attatggacc gcattattca atcaccgggt aaatacatcc agggcgctga
    27241 tgtgattaat cgtctgggcg aatacctgaa gccgctggca gaacgctggt tagtggtggg
    27301 tgacaaattt gttttaggtt ttgctcaatc cactgtcgag aaaagcttta aagatgctgg
    27361 actggtagta gaaattgcgc cgtttggcgg tggatgttcg caaaatgaga tcgaccgtct
    27421 gcgtggcatc gcggagactg cgcagtgtgg cgcaattctc ggtatcggtg gcggaaaaac
    27481 cctcgataca gccaaagcac tggcacattt catgggtgtt ccggtagcga tcgcaccgac
    27541 tatcgcctct accgatgcac cgtgcagcgc attgtctgtt atctacaccg atgagggtga
    27601 gtttgaccgc tatctgctgt tgccaaataa cccgaatatg gtcattgtcg acaccaaaat
    27661 cgtcgctggc gcacctgcac gtctgttagc ggcgggtatc ggcgatgcgc tggcaacctg
    27721 gtttgaagcg cgtgcctgct ctcgtagcgg cgcgaccacc atggcgggcg gcaagtgcac
    27781 ccaggctgcg ctggcactgg ctgaactgtg ctacaacacc ctgctggaag aaggcgaaaa
    27841 agcgatgctt gctgccgaac agcatgtagt gactccggcg ctggagcgcg tgattgaagc
    27901 gaacacctat ttgagcggtg ttggttttga aagtggtggt ctggctgcgg cgcacgcagt
    27961 gcataacggc ctgaccgcta tcccggacgc gcatcactat tatcacggtg aaaaagtggc
    28021 atttggtacg ctgacgcagc tggttctgga aaacgcaccg gttgaggaaa tcgaaaccgt
    28081 agctgcactt agccatgcgg taggtttgcc aataactctc gctcaactgg atattaaaga
    28141 agatgtcccg gcgaaaatgc gaattgtggc tgaagcggca tgtgcagaat gtgaaaccat
    28201 ccacaacatg cctggcggcg cgacgccaga tcaggtttac gccgctctgc tggtagccga
    28261 ccagtacggt cagcgtttcc tgcaagagtg ggaataacct actccaaact cccggcttgt
    28321 ccgggagttt gaacgcaaaa ttgcctgatg cgctacgctt atcaggccta cgcaatctct
    28381 gcaatatatt gaattttctt gttttttagg acagataagg cgttcacgcc gcatccggca
    28441 ctctgtgcca gtaatctgaa actcccacgt tgttcaggag ttttttatcc gcttctggac
    28501 attttctcta cagaacaatc gttatcccgg aaggtcgaat ctgtgactct tattttcacg
    28561 gacgaaccag atgaaagctt cactggcgct gctcagcctg ctaacggcat ttaccagcca
    28621 ctccttaaaa tctcccgccg ttccaccaac cgtggtacaa atccaggcca ataccaacct
    28681 tgccatcgca gacggtgcca gacagcaaat cggcagcacg ctattttacg atccggcgta
    28741 tatgcagctt acctatcctg gcggtgatgt cccgcaaaaa cgcggggtat gttccgatgt
    28801 ggtgatccgt gcattgcgca gccagaaggt cgatttgcag aaactggtgc acgaagatat
    28861 ggcgaagaat tttgccgagt acccgcaaaa atggcagtta aagcgcccgg acagcaacat
    28921 cgatcaccgt cgggtgccta atctggaaac ctggtttacc caccacgata aaacacgccc
    28981 catcagcaag aaccccagtg actatcaagc gggcgatatt gtctcctggc gattagataa
    29041 cgggctggca catattgggg tggtttcaga tggcttcgcc cgcgacggta cgccgctggt
    29101 gatacataac attggcgcag gtgcgcagga agaagatgtg ctgtttagct ggcgcatggt
    29161 ggggcattat cgttattttg ttaaataagc gatcgcccca catgaagaaa aaattcagat
    29221 cctttttaca ctaacggctt cttttttctt acggctcacc agcgccagtg ccatcacaat
    29281 cagcacaata ccgctacctt caacggcccc cggattctcg ccgagcagcc accaggaaaa
    29341 gagtacgccg caaacgggaa cggccagtgt gcttaagctg gcaatactgg caggcaagtt
    29401 tttcaataca aacaaccata agctccacgc cagtgccgtc gccagaatcg cactgtaggc
    29461 cagcgcccag aacacggtgg gctgccagtc aatttcacgt tgcggcacca gtaaagcgac
    29521 cacactcatc accagcgccg catagagcat ctgccaggat gttaacgaca ataaatccac
    29581 gcgcggatga cgggcataca gacgtttagc aacaatcgcg ctcgcccccc aactgacgcc
    29641 ggagaggatt gccagcatgg cacttttcat cgaagagaaa tcgagttgcc acggctgcaa
    29701 caccaaaaat aagccgaaag cggcaatcag tatcgcgaaa tattgcccac gtcgcaggcg
    29761 ttcaccgaga aacaacgcgg cgaaaatcac cacccagaac ggcatggtat agcttaggat
    29821 cgccaccttc cccgctccgc cgctgaccag cgcccactgc gccaggccaa ccatcccgca
    29881 ggtttgtaac agggcaatgg ctaaggtgta tttaaacggt gtcgggcgca ttccgcgacc
    29941 acgtaataaa aggacgatga ataaaacgag agcgccgaaa atgcagcgta aggcggtaaa
    30001 gtcgaaggca ccgatgtagc tggtgacttg cttcatgaaa atccagctat aactccagat
    30061 aagtgtgagc acaaccaggc cactgattgc cagtgggttg ctctttcctg cggcagacat
    30121 gagagatcca gtgtgtagtt ttcctgtgga cactatacgc cggtaaagaa acttcagcga
    30181 ctgcctgaaa ggcagtcgct gaagaaggtc agattacagc aggtcgaaac ggtcgaggtt
    30241 catcactttc acccatgccg ccacgaagtc tttaacaaac ttctcgtgag catcgctgct
    30301 ggcgtaaact tcagccaccg cacgcaggac agagttagaa ccaaatacca gatccgcacg
    30361 gctggcagtg tatttcactt cgccggtttc gcggtcacgg ccttcaaaca gctctttcga
    30421 ttcgtcggtc gctttccact cgtaacgcat atccagcaag ttcacgaaga agtcattgct
    30481 caatacgcca acgcggtcag tgaagacgcc gtttttgctg ccatcgaagt tggcacccag
    30541 tacgcgcatc ccgcccacca gcgcggtcat ttccggcgcg gtcagggtca gttgctgggc
    30601 tttgtcgatc agcagtgact cggtggtgga aacatccaga cgagcgcgat agttacggaa
    30661 accgtcagca attggctcca gcagctcgaa catctcaatg tcagtctgat cctgacgcgc
    30721 atcaacgcgg cccggcgcaa acggtacatg aatgctcaaa cctgcggcgc ttgcggcttt
    30781 ctcaacacca accacaccag ccagcacgat gatatccgcc agcgaggctt taccagactc
    30841 tttctggatt ttctccagaa caggcagagc acgaacggct gcggcgttca catcccagtc
    30901 gcgctgcggc attaatgcca gacgcgcacc gttggcacca ccgcgtttgt cgccaccacg
    30961 gaaggtagaa gcagatgccc aggccaccga tacccgctca ctaacagaca gaccagaatc
    31021 cgcaatcgcg aatttcagat cgataatgtc ctgctcggtc gggttgtaga tcggctgcgg
    31081 cagcggatct tgccagatca gatcttcttt cggcacttcc ggcccgatgt agcgagattt
    31141 cggccccata tccctgtgcg tcagtttgaa ccaggcacgg gcaaaggctt cgttgaacgc
    31201 ctgcggatcg ttgaggaaac gacgagagat cttctcgaac tcaggatcaa aacgcagcgt
    31261 caggtcggtc accagcatcg tcggtttacg tttcttcgac ggatcgaatg gatccgggat
    31321 aatttccggt gcgtctaccg cttcgaactg gattgcgcca gccggactgc gggtctgtac
    31381 ccactcatat ttgaacaggt tttcgaagaa atagttgctc cactgggtcg gcgtctgggt
    31441 ccagacaact tccagaccag aggtaatggc atctgcgcca acgccgctgc cgtaagtgct
    31501 cgcccagcct aaaccttgtt cttcaatcgg tgcagcttct ggatcaggac ctacgtttga
    31561 tgtcggaccg gcaccgtggg ttttacccag cgtatgacca cccgcaatca gcgccacggt
    31621 ttcttcgtcg ttcatgccca tgttgccgaa ggtcgcgcgg atagctgctg ccgcagaaag
    31681 cggttcgccg ctgtgatccg ggccttccgg gttaacgtaa atcaggccca tctcggttgc
    31741 acccagcggt gctttcgcca gcgcttccgg atgacggtga gtcagccagg ctttttcatc
    31801 accccagtta acatccagat ccggttccca gacgtcttca cgaccggcac caaaaccgaa
    31861 ggtacggaag ccggagtttt ccagcgccac gttacctgcg aggataaaca ggtcggccca
    31921 ggagattttc tgaccatatt tctgtttgat tggccacaac aggcgacgcg ctttatcgag
    31981 gcttacgtta tccggccagg agttcagcgg tgcaaaacgt tgctgaccac gacccgcgcc
    32041 gccgcgtcca tcgattgaac ggtaagtacc tgcgccgtgc caggccatac gaataaacag
    32101 accggcgtag ctgccccagt cggctggcca ccacggctga gattctgtca gcagggcttt
    32161 cagatctttt ttcaggccgt agtaatctaa tttgctgaat tctttgcggt agtcaaagtc
    32221 ctcacccagc gggttagaac gattagaatg ttggtttaac aggtcaacac ggagttgatt
    32281 tggccaccag tcgcgagtgg ttgtgcccgc ccccgcgctc tggtcgtgac cgccctgatg
    32341 gaacgggcat ttgccagtgg ctgtcgtgtt atggatatcg tctgacgtgc tcatcaatgt
    32401 gctcccctct acagtgttac cgttacgata cacagcgtta gagagaagtt ataattgaat
    32461 taatccacgg atgcgatagt tgagatctta catattgttg gttaaagaga tgtaggtcaa
    32521 attgatcgta attaagcttt tttgattatg tccgaattcg gacaaatgct ttataaaaaa
    32581 gggttttagc ttcatatcct tcaagtaatg agggagaaaa caaggctgac aaccttattt
    32641 tcccgccctc atttcgaggc agcattttgt gctctgttta aaatttgtga tcactgtgtg
    32701 attttcacaa aagccacact atttataaac caggtcgaac acccagcgta tggcaaatcg
    32761 cgtaactcat ttcagcgcgg ttaagcgtat agaagtggaa atctttcact ccttcacggc
    32821 ttaaaatctt caccatatcc atggcaatat tcgcgcccac cagtttgcgg gtttcggcat
    32881 catcatccag gccgtcgaac atttgcgcca tccacgccgg aatacgcacg ttggtcatat
    32941 cggcaaattt cttcgcctgt ttaaagttag ataccggcaa aattcccgga ataatttcca
    33001 catcaatgcc cgccgataca cagcggtcac gaaaacgcag gtagctttcg acatcgaaga
    33061 agaactgagt aatcgcgcgg ttggctccgg catcgacttt gcgtttcaga ttaagcaaat
    33121 ccgcctgagc gctttttgct tccgggtgaa cttccggata cgccgccacg gagatatcga
    33181 aatctgccac ttcttttaat agcgtcacca ggtcagaagc atacatttct ggcttaccac
    33241 ttcccggcgg cagatcgcca cgcagcgcca cgatatgacg aataccgtta ttccagtagt
    33301 cgcgtgcaat ggtgcgcaac tcgtcgggcg tcgcatcaat gcaagtaaga tgcggtgccg
    33361 cttccagacc agtgcgatct ttaatgcctt taataatgct gtgcgtacgg tcgcgctcgc
    33421 cggagttcgc gccataggtc accgatacaa acttcggttt caggctgcta aggcgatcga
    33481 tggagttcca cagggtctgc tccatttcac tggtacgcgg cgggaaaaac tcgaacgaaa
    33541 cgttaatctg cccctggact tctgccaggc tctgattcag ggcatcccgc tggctggcgt
    33601 gaaaaaagct cataccttac ctcatcaatc gcttgtagtt atatgttgtg ttgtgcacat
    33661 ctatccgttt agacgtccag atgtaaagat gaaggaaaag ccgaagggcg tcaaccgaaa
    33721 aatgaccaat gatggtgagg aaaaatcagg gaagatgaaa aaacttcagg atggtaagaa
    33781 aaagaaaatc cggtatgcaa agcaaaccgg actgcaaata taaactaacc atgtacttat
    33841 tgcttcttgc cggattcgtc gtaaacgctt aatccggcct aatcctatga taattcaata
    33901 tatttcatta gaaaccataa acccgaaaac atgagtaccg ggcattatta aatttctgaa
    33961 attacaacaa ctgtgccagc cggttgatat ccgactgaat cgccccggcg gtgacgtcgc
    34021 gcccagcgcc aggcccacgg atcaccagcg gattatcgcg ataccagcgg ctttcgatgg
    34081 caaagacgtt atcgcacggc agcagtgatg ccagcggatg gtcttcacgc accgcttcca
    34141 cgcccacacg cgctttgcca ttggcatcga aacgcgccac gtagcgcagc accagcccca
    34201 tttcgcgggc cgcttccagc cgttgcacca tctgctcgtt aagttcatcg ccattttcaa
    34261 agaaatggtc gatgctgccg ccttcgcaat gggcaggcac cagcgactcc acgcgtacct
    34321 gatccggttc gatgttgtaa cctgcttcac gcgccagaat caccagcttg cgcatcacgt
    34381 ctttgccaga gagatcgtca cgcgggtcag gttcggttaa gccctgctgc cacgcctgat
    34441 ccaccagctc ggtaaacggc acgctaccgt cgaattgcag gaacaaccag gagagcgtgc
    34501 cggagaagat cccgctgatc gacaaaatag tatcgccgct gtcgatcaga tcgcgcacgg
    34561 tgtggttgat cggtaagccc gcaccgacgg tggcattgta cagccagtga cgcccggttt
    34621 tttcgaaggc gtcgtggatc tggcgatatt tattgctgtc gctggctccc gccagtttgt
    34681 tggcgctgat gacgtggaaa ccgtggctgg cgaaatcaag atactgatca gcaagctgct
    34741 ggctggcggt aacgtccagc accactaaat catcatacgg atgggcgcgc atccacagga
    34801 acaacgactc ttcatcctgc tcaaccgctt catcgttgaa gaaggctaac gcgcggctgg
    34861 cgtccagccc gtcatagctc aacagactgc ggcggctgtc caccacgcct gccagcacaa
    34921 attcaaagtc ggtacgtgcc gaaagcgtgc tctgctcacg ggcgaacagt tccagccaac
    34981 gggaaccgat attgccctta ccgaacaata ccaggccgat gcgtttttct gcgcggaaga
    35041 cggactgatg cagcccctga atcaggcttt cggtcgggcc ggtgcgcagt actgccacca
    35101 ggctgatgcc gtcatcggac tgccaggtaa attcgaccgg ctggcctttc agttgctgcc
    35161 agaagcggtg gcaatgcagc gggttacggg tgacgcctgc accgaccatc gccaccagcg
    35221 ccagcccctg acgcaggcgc agttcgccag gtaatcccgc ttcgtcgagg attttcagcg
    35281 cactgtcggc cacttctgag gtgtagcaaa attgcagcaa ctggcgatcg ttatgtacgc
    35341 caaccgccag cgggcgtacc tgcgcgcgtt tcaggatttg gtcgatctct ttatgcgcca
    35401 gtttgaaatc ctgactggcg ggcacctgaa actcaatcaa acagacatca tcgtggctgg
    35461 tgacaatacg cgcaccagta ccggaggcca gcacgcgttc aatgcgcgtg gaaccttgat
    35521 ccggcgtgta gctacagcgc agttgcaggt cgatttcgct gccagaaacc ggctgtaaag
    35581 tacgggcgtg aagaacggga gccgccagac gagccagttc gctggcttca tccagacgca
    35641 gcaacggcag cagacaggca tctttcactt tacgcgggtc ggcactgtat accccggcga
    35701 cgtcgctcca gatggttaca cgagaaacac ccgccagcgc accgatttgt gtcgcggaat
    35761 agtcggaacc gttacgcccc agcagcaccg tttcaccggc gttgttacgg ctgataaacc
    35821 cggtcaccac cagacgtttg cccggatgtt gcaccagtag ctgttgcagc aacgggtaag
    35881 aaagcccttc atcaacctgc ggttgtgcgg cgcgttcagc gcgtaaaaac tcgcgggcat
    35941 caagccaggc cgctggcagc ccttgttgat taagtaccgc agacatcagg cgtgccgacc
    36001 atacttcccc gtggcccacc acttccgcat acactgcgtc gttaataccg ctgtcgagca
    36061 gcgccgccag gcgttccaga tcgctgacaa aagcgctaat gaggctatcg gcttcttcag
    36121 cgggtagcag accgctaatc agatcgcact gatagcgacg cagcgtttgt tgaacctgat
    36181 gcgcagagag acgatcggtc tggcttagtt tcaaccagtt aatcaactgg ttagtggtgc
    36241 taccggcggc ggaaaccacc atcatatcgt caggctgaga gtactccgcc ataatgcccg
    36301 cgacacgcaa ataacacttc acatcagcca gactactgcc accaaattta tgcagctgac
    36361 gacctttcgc ccctgcctgc gcaatcacac tcatttttac cccttgtttg cagcccggaa
    36421 gccattttcc aggtcggcaa ttaaatcttc gccatcttca ataccggtgg agatacgcag
    36481 cagcgtctcg gagatcccga cggcagcacg cgcttctggt gccatgcctg catgtgtcat
    36541 ggttgcggcg tgagagatta aactttccac tccccctaat gattccgcca gcgtaaacaa
    36601 cgacagcccg ctcaggaaac gacgcagcgt ctgctcatcg ccatccagtt caaaactcaa
    36661 cattgcgcca aagccttttt gctgacgcgc ggcaatttca tgcccctgat tttccggcag
    36721 cgacgggtga tacagttttt tcaccaacgg ctgggtttgc agatatttca caatcgcctg
    36781 cgcgttgcgc tgcgccagct ccatacgcgg caccagcgtt cgcaacccgc gtagcagcag
    36841 atagctgtca aacgcgccgc ccgtcacgcc aatattgttt gcccaccagg caagttcagt
    36901 gacaacgtcc gggtctttag caatcaccac gccagccacc acgtcagagt gaccgttcag
    36961 atatttcgtg catgaatgca acaccagatc ggcacctaat gccagcggat tttgtaatgc
    37021 cgggcttaag aaggtgttat ccaccacgct caccgccccg acttcccttg ccagatggca
    37081 gattttcgca atatccacga cgcgtaacaa tggattactt gggctttcta ccagtaccag
    37141 tttgggtttt tctgccagcg ctgcccgtaa tgcctgttca tcgccttgat caacaaacaa
    37201 cacgcgatag caaccgcgtt tcgccagact gtcgaacagg cgatagctac cgccgtagca
    37261 gtcgtgcggc gcaaccagca gatcgccagg tttcaaaaag acggtcgtta ccaggtgaat
    37321 cgcggacatg ccggtattag taagtactgc gccagcaccg ccttccagtt ctgccagcgc
    37381 acgctgaacc acatcgcgcg ttgggttgcc gcgacgcgag taatcatgcg cgcgcggttc
    37441 attaaatccg gtaaagttat aggtgctgga aagatggatg ggagggacaa cgcaaccata
    37501 ctgttcgtcg tcatttaacc cgctacgcac tgcgatggtg gcctgtttac gcgtcatgtg
    37561 atgaagtttc ctgggctttg tcggtgaaat gtcaggcacc agagtaaaca ttgtgttaat
    37621 ggacgtcaat acatctggac atctaaactt ctttgcgtat agattgagca aatcccaaat
    37681 agccgttaaa attatatgca ttatcacgcc gacaggtgca ttacacgatg tcacggtaac
    37741 gcctgtacgg taaactatgc gggtttacgg tcagtaccca cataaattgt gtggtctggt
    37801 ctcaatttat tgacgaagag gattaagtat ctcatggctg aatggagcgg cgaatatatc
    37861 agcccatacg ctgagcacgg caagaagagt gaacaagtca aaaagattac ggtttccatt
    37921 cctcttaagg tgttaaaaat cctcaccgat gaacgcacgc gtcgtcaggt gaacaacctg
    37981 cgtcacgcta ccaacagcga gctgctgtgc gaagcgtttc tgcatgcctt taccgggcaa
    38041 cctttgccgg atgatgccga tctgcgtaaa gagcgcagcg acgaaatccc ggaagcggca
    38101 aaagagatca tgcgtgagat ggggattaac ccggagacgt gggaatacta aagcaaaaaa
    38161 gagcggcgcg gagtggaatc gcctgatgcg ctacgcttat caggcctacg tcatattgca
    38221 atttattgaa tttgcacgaa cttgtaggcc ggataaggcg ttcacgccgc atccggcata
    38281 aacaacgagc acgttgtctg cgacccaccg ctttttatac atggacgttt aactatgaaa
    38341 aacaggctgc tgatcctcag cctgctggtt tctgtacctg cctttgcctg gcagccacaa
    38401 accggcgaca tcatctttca gatctctcgc tcatcgcaaa gtaaagcgat ccaactggcg
    38461 acccattccg attatagcca caccggtatg ctggtgatac gcaacaaaaa gccctacgtt
    38521 tttgaagcgg tcggcccggt gaaatacacc ccgctcaagc agtggatcgc ccatggtgaa
    38581 aagggcaaat acgttgttcg ccgcgttgaa ggcggactga gtgttgaaca acagcaaaaa
    38641 ctggcgcaaa cggcaaaacg ttatctcggt aaaccgtatg atttcagctt ctcatggagc
    38701 gacgatcgcc agtactgttc ggaagtggtg tggaaggttt accagaacgc gctgggaatg
    38761 cgcgtgggcg agcagcagaa gttgaaagag ttcgatctca gcaacccgct ggtccaggct
    38821 aagctcaaag aacgctacgg caaaaatatt ccgcttgagg aaacggtagt ttcaccacaa
    38881 gccgttttcg acgcgccaca actcaccaca gtcgccaaag agtggccgct gttttcgtgg
    38941 taatggatga tgaaaaaaat aattcccgcc gtagtctgtt tgttgctttg tagttgcacg
    39001 acctttccgc aagctgtcgc tccggtaaac ggtgatgcct ccaattagtt gaattaagga
    39061 tataatactc gcttttaagg ttgtttcatg gtcacagtta accttcattg cccccgttgc
    39121 cagtccgttc aggtttaccg tcacgggcag aacccaaaaa gacatgagcg attccgcggc
    39181 cgtgactgtc accgggtatt tcagctcagt tactgttacg agccccgtaa accgggagtt
    39241 aaagaccaaa tcgctgaaat gactttcaac ggtgccgggg ttcgcgatac tgcccgtacg
    39301 ctgaaaatcg gcattaatac agtcatccga actttaaaaa actctcgcca cgaagaataa
    39361 cgtcatcccc ggtcgctcat gctgatgtgg cactcatctg caaacttagt cagcaatgga
    39421 gctttgtcgg cagtaaagcg cgacagcact ggctctggta cgtgtacaac acgaaaactg
    39481 gtggtgttct ggcctacact tttggccccc ggactgacga aacctgtcgt gaattgcggg
    39541 cgctgcttac ccttttacca tcggcatgtt gaccactgac gaatggggta gttacgctag
    39601 agagttaccg aaggagaagc atctgactgg taagattttt actcagcaca ttgagcgtaa
    39661 caatctgacc ctgagaaccc gaatcaaacg tctggcccgc agaaccatct gtttctcgcg
    39721 ttctgtagaa cttcatgaaa aagtcatcgg tgcttttatc gaaaatatat gttctactaa
    39781 ttggagtcat cacccttctg ataagctttc cttggatgat taccagactg aatacacagc
    39841 caaagatttt tacctcattc tgctcttttt tctcaaatga catatattat attttacatc
    39901 atattcatag gcatatgaca ttcccccgcc ccccattatg tcattgtggg ttgatgttgg
    39961 gctttcataa aaaaaacatc tttttttgaa ctagcattaa ttatatttac tttatatatt
    40021 aaaatatttt ctccatctgg aaatatttta gccacacaat ataaagcagc tgaatccgaa
    40081 ttacttaaaa atatattatc atcacccata actccagcca ttaaatgact ggagactaaa
    40141 gcaaggaaat aacaacattg tattgccttc attttaatta ctcccttgcc tgataattgt
    40201 aactccattc cctttagctt tgggattata ttttagatag gtgttatttg gtgttcccaa
    40261 gtaactactg tattcttttt ttcccattcc attcatataa gaattcgtta aatctttgct
    40321 tgaaaaattt agactatcat aaacatcatt ttcttttgtt actttatttc ctttatcgtc
    40381 agagtaaaaa ccgtgagtgt gataatcgcc tactctctct gaaccttcag ggcattttat
    40441 atttcgcaag tctacactat cattatcatt tgaacttatc ggattcatgg ggaaatattc
    40501 accttgcttc ttacatatta gtccaccata ttctttattc tcgttaattg attctccatt
    40561 acacattgcc aatgcaaata ttccaatatc atcaatattt tttgatttaa attcatataa
    40621 tcctaatgga tccatgctat tcaccgggct cagcggatat gtatacaggt tccatccccc
    40681 cttcagccca atcggatcct gagtgatata ccgcccctgc agcgggtcat aatagcggtg
    40741 gcggttataa tacaggccgg actcctcatc atactgctgc cccggcaaac gaataagctg
    40801 ctgcagctga tgcgggttct cttcattcag caggttgccc cattcatcat attcccggcg
    40861 tatttccagc ccgtcaggcc cggtggctga cgttaactgg ctgtggtggt tatagtaaaa
    40921 cgccgatgcc ctgccatccg gcgtggtgat gcgcgtgatg agacccgtca ccacatccgg
    40981 gctgtattct gttgtccggc ctgcggcatc cgtctgtgtc ctgagcctgc ccacggcgtc
    41041 aaactgactc tgcgtgacgc tgccgtccgc gtgttccttt ttcaccaccc gcttcagccc
    41101 ggcttcaccc tacgtgt
//
LOCUS       DAZPEP010000021        39145 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZPEP010000021 DAZPEP010000000
VERSION     DAZPEP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39145
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.021"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..266
                     /locus_tag="ACHUT9_001088"
     CDS             <1..266
                     /locus_tag="ACHUT9_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFR1347619.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSLVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="ACHUT9_001089"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="ACHUT9_001089"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFR1347620.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUT9_001090"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="ACHUT9_001090"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFR1347621.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="ACHUT9_001091"
     CDS             complement(1570..3270)
                     /locus_tag="ACHUT9_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFR1347622.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="ACHUT9_001092"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="ACHUT9_001092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFR1347623.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="ACHUT9_001093"
                     /pseudo
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="ACHUT9_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
     gene            4828..6600
                     /locus_tag="ACHUT9_001094"
     CDS             4828..6600
                     /locus_tag="ACHUT9_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1347624.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="ACHUT9_001095"
     CDS             6593..8374
                     /locus_tag="ACHUT9_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFR1347625.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUT9_001096"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="ACHUT9_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFR1347626.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUT9_001097"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="ACHUT9_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFR1347627.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUT9_001098"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="ACHUT9_001098"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFR1347628.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="ACHUT9_001099"
     CDS             11336..11908
                     /locus_tag="ACHUT9_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFR1347629.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="ACHUT9_001100"
     CDS             complement(11939..12250)
                     /locus_tag="ACHUT9_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFR1347630.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="ACHUT9_001101"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHUT9_001101"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="ACHUT9_001102"
     CDS             12629..12982
                     /locus_tag="ACHUT9_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFR1347631.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUT9_001103"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="ACHUT9_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="ACHUT9_001104"
     CDS             complement(14736..15206)
                     /locus_tag="ACHUT9_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFR1347632.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUT9_001105"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="ACHUT9_001105"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFR1347633.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /locus_tag="ACHUT9_001106"
     CDS             complement(16044..16262)
                     /locus_tag="ACHUT9_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFR1347634.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUT9_001107"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="ACHUT9_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFR1347635.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHUT9_001108"
     CDS             complement(17208..20357)
                     /gene="acrB"
                     /locus_tag="ACHUT9_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFR1347636.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHUT9_001109"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="ACHUT9_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFR1347637.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="ACHUT9_001110"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="ACHUT9_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFR1347638.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="ACHUT9_001111"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="ACHUT9_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFR1347639.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            25913..26011
                     /locus_tag="ACHUT9_001112"
                     /pseudo
     CDS             25913..26011
                     /locus_tag="ACHUT9_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHUT9_001113"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="ACHUT9_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFR1347640.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHUT9_001114"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="ACHUT9_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFR1347641.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="ACHUT9_001115"
     CDS             26836..27213
                     /locus_tag="ACHUT9_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFR1347642.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="ACHUT9_001116"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="ACHUT9_001116"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFR1347643.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHUT9_001117"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="ACHUT9_001117"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFR1347644.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="ACHUT9_001117"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="ACHUT9_001118"
     CDS             30024..30353
                     /locus_tag="ACHUT9_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFR1347645.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="ACHUT9_001119"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="ACHUT9_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFR1347646.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="ACHUT9_001120"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="ACHUT9_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFR1347647.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="ACHUT9_001121"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="ACHUT9_001121"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFR1347648.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="ACHUT9_001122"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="ACHUT9_001122"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFR1347649.1"
                     /translation="MRQTKIGILLANLGTPDTPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHUT9_001123"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="ACHUT9_001123"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFR1347650.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="ACHUT9_001124"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="ACHUT9_001124"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFR1347651.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHUT9_001125"
     CDS             complement(37406..39082)
                     /gene="ybaL"
                     /locus_tag="ACHUT9_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFR1347652.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9436 a        10008 c        10358 g         9343 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg ctggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 gcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgtaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaactg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtcgcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccggaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgcctcat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgata cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaa
//
LOCUS       DAZPEP010000022        38337 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZPEP010000022 DAZPEP010000000
VERSION     DAZPEP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.022"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            59..1423
                     /locus_tag="ACHUT9_001126"
     CDS             59..1423
                     /locus_tag="ACHUT9_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1347653.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUT9_001127"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="ACHUT9_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFR1347654.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="ACHUT9_001128"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="ACHUT9_001128"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFR1347655.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUT9_001129"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="ACHUT9_001129"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFR1347656.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUT9_001130"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="ACHUT9_001130"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFR1347657.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUT9_001131"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="ACHUT9_001131"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFR1347658.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUT9_001132"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="ACHUT9_001132"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFR1347659.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUT9_001133"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="ACHUT9_001133"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFR1347660.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUT9_001134"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="ACHUT9_001134"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFR1347661.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUT9_001135"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="ACHUT9_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFR1347662.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUT9_001136"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="ACHUT9_001136"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFR1347663.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUT9_001137"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="ACHUT9_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFR1347664.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUT9_001138"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="ACHUT9_001138"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFR1347665.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUT9_001139"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="ACHUT9_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFR1347666.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUT9_001140"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="ACHUT9_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFR1347667.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="ACHUT9_001141"
     CDS             13749..14288
                     /locus_tag="ACHUT9_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFR1347668.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUT9_001142"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="ACHUT9_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFR1347669.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUT9_001143"
     CDS             complement(15648..15995)
                     /gene="yajD"
                     /locus_tag="ACHUT9_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFR1347670.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUT9_001144"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="ACHUT9_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFR1347671.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUT9_001145"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="ACHUT9_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFR1347672.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUT9_001146"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="ACHUT9_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFR1347673.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUT9_001147"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="ACHUT9_001147"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFR1347674.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUT9_001148"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="ACHUT9_001148"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFR1347675.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUT9_001149"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="ACHUT9_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFR1347676.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUT9_001150"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="ACHUT9_001150"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFR1347677.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUT9_001151"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="ACHUT9_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFR1347678.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUT9_001152"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="ACHUT9_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFR1347679.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUT9_001153"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="ACHUT9_001153"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFR1347680.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUT9_001154"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="ACHUT9_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFR1347681.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUT9_001155"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="ACHUT9_001155"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFR1347682.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUT9_001156"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="ACHUT9_001156"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFR1347683.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLTLENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUT9_001157"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="ACHUT9_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFR1347684.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUT9_001158"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="ACHUT9_001158"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFR1347685.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUT9_001159"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="ACHUT9_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFR1347686.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="ACHUT9_001160"
     CDS             complement(37465..37920)
                     /locus_tag="ACHUT9_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347687.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="ACHUT9_001161"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="ACHUT9_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9466 a        10313 c         9734 g         8824 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacgc ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactgc cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcta
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctggcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ccgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactgacgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaagcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggac gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAZPEP010000023        38163 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZPEP010000023 DAZPEP010000000
VERSION     DAZPEP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.023"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            40..114
                     /locus_tag="ACHUT9_001162"
     tRNA            40..114
                     /locus_tag="ACHUT9_001162"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Gln,seq:ctg)
     gene            complement(268..1443)
                     /gene="ubiF"
                     /locus_tag="ACHUT9_001163"
     CDS             complement(268..1443)
                     /gene="ubiF"
                     /locus_tag="ACHUT9_001163"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFR1347688.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            1589..3013
                     /gene="miaB"
                     /locus_tag="ACHUT9_001164"
     CDS             1589..3013
                     /gene="miaB"
                     /locus_tag="ACHUT9_001164"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFR1347689.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            3166..4206
                     /locus_tag="ACHUT9_001165"
     CDS             3166..4206
                     /locus_tag="ACHUT9_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFR1347690.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            4203..4670
                     /gene="ybeY"
                     /locus_tag="ACHUT9_001166"
     CDS             4203..4670
                     /gene="ybeY"
                     /locus_tag="ACHUT9_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFR1347691.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            4760..5638
                     /gene="corC"
                     /locus_tag="ACHUT9_001167"
     CDS             4760..5638
                     /gene="corC"
                     /locus_tag="ACHUT9_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFR1347692.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            5663..7201
                     /gene="lnt"
                     /locus_tag="ACHUT9_001168"
     CDS             5663..7201
                     /gene="lnt"
                     /locus_tag="ACHUT9_001168"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFR1347693.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(7279..9156)
                     /locus_tag="ACHUT9_001169"
     CDS             complement(7279..9156)
                     /locus_tag="ACHUT9_001169"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFR1347694.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            9611..10519
                     /gene="gltI"
                     /locus_tag="ACHUT9_001170"
     CDS             9611..10519
                     /gene="gltI"
                     /locus_tag="ACHUT9_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFR1347695.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            10689..11429
                     /gene="gltJ"
                     /locus_tag="ACHUT9_001171"
     CDS             10689..11429
                     /gene="gltJ"
                     /locus_tag="ACHUT9_001171"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFR1347696.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            11429..12103
                     /gene="gltK"
                     /locus_tag="ACHUT9_001172"
     CDS             11429..12103
                     /gene="gltK"
                     /locus_tag="ACHUT9_001172"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFR1347697.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            12103..12828
                     /gene="gltL"
                     /locus_tag="ACHUT9_001173"
     CDS             12103..12828
                     /gene="gltL"
                     /locus_tag="ACHUT9_001173"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFR1347698.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            12946..13881
                     /gene="rihA"
                     /locus_tag="ACHUT9_001174"
     CDS             12946..13881
                     /gene="rihA"
                     /locus_tag="ACHUT9_001174"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFR1347699.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            13973..15643
                     /gene="hscC"
                     /locus_tag="ACHUT9_001175"
     CDS             13973..15643
                     /gene="hscC"
                     /locus_tag="ACHUT9_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFR1347700.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(15703..17154)
                     /gene="djlC"
                     /locus_tag="ACHUT9_001176"
     CDS             complement(15703..17154)
                     /gene="djlC"
                     /locus_tag="ACHUT9_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFR1347701.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(17151..17858)
                     /locus_tag="ACHUT9_001177"
     CDS             complement(17151..17858)
                     /locus_tag="ACHUT9_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1347702.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            17960..18514
                     /locus_tag="ACHUT9_001178"
     CDS             17960..18514
                     /locus_tag="ACHUT9_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1347703.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(18524..19951)
                     /locus_tag="ACHUT9_001179"
     CDS             complement(18524..19951)
                     /locus_tag="ACHUT9_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFR1347704.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(19948..20655)
                     /locus_tag="ACHUT9_001180"
     CDS             complement(19948..20655)
                     /locus_tag="ACHUT9_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1347705.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            20819..21796
                     /gene="ybeQ"
                     /locus_tag="ACHUT9_001181"
     CDS             20819..21796
                     /gene="ybeQ"
                     /locus_tag="ACHUT9_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFR1347706.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(21866..22348)
                     /locus_tag="ACHUT9_001182"
     CDS             complement(21866..22348)
                     /locus_tag="ACHUT9_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFR1347707.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            22583..25165
                     /gene="leuS"
                     /locus_tag="ACHUT9_001183"
     CDS             22583..25165
                     /gene="leuS"
                     /locus_tag="ACHUT9_001183"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFR1347708.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            25180..25761
                     /gene="lptE"
                     /locus_tag="ACHUT9_001184"
     CDS             25180..25761
                     /gene="lptE"
                     /locus_tag="ACHUT9_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFR1347709.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            25761..26792
                     /gene="holA"
                     /locus_tag="ACHUT9_001185"
     CDS             25761..26792
                     /gene="holA"
                     /locus_tag="ACHUT9_001185"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFR1347710.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            26794..27435
                     /gene="nadD"
                     /locus_tag="ACHUT9_001186"
     CDS             26794..27435
                     /gene="nadD"
                     /locus_tag="ACHUT9_001186"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFR1347711.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            27459..28070
                     /locus_tag="ACHUT9_001187"
                     /pseudo
     CDS             27459..28070
                     /locus_tag="ACHUT9_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            28330..28647
                     /gene="rsfS"
                     /locus_tag="ACHUT9_001188"
     CDS             28330..28647
                     /gene="rsfS"
                     /locus_tag="ACHUT9_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFR1347712.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            28651..29118
                     /gene="rlmH"
                     /locus_tag="ACHUT9_001189"
     CDS             28651..29118
                     /gene="rlmH"
                     /locus_tag="ACHUT9_001189"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFR1347713.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            29149..31050
                     /gene="mrdA"
                     /locus_tag="ACHUT9_001190"
     CDS             29149..31050
                     /gene="mrdA"
                     /locus_tag="ACHUT9_001190"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFR1347714.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            31053..32165
                     /gene="mrdB"
                     /locus_tag="ACHUT9_001191"
                     /gene_synonym="rodA"
     CDS             31053..32165
                     /gene="mrdB"
                     /locus_tag="ACHUT9_001191"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFR1347715.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            32176..33264
                     /gene="rlpA"
                     /locus_tag="ACHUT9_001192"
     CDS             32176..33264
                     /gene="rlpA"
                     /locus_tag="ACHUT9_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFR1347716.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            33403..34614
                     /gene="dacA"
                     /locus_tag="ACHUT9_001193"
     CDS             33403..34614
                     /gene="dacA"
                     /locus_tag="ACHUT9_001193"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFR1347717.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            34724..34987
                     /gene="ybeD"
                     /locus_tag="ACHUT9_001194"
     CDS             34724..34987
                     /gene="ybeD"
                     /locus_tag="ACHUT9_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFR1347718.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            35088..35729
                     /gene="lipB"
                     /locus_tag="ACHUT9_001195"
     CDS             35088..35729
                     /gene="lipB"
                     /locus_tag="ACHUT9_001195"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFR1347719.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            35989..36942
                     /locus_tag="ACHUT9_001196"
     CDS             35989..36942
                     /locus_tag="ACHUT9_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFR1347720.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            37151..38116
                     /gene="lipA"
                     /locus_tag="ACHUT9_001197"
     CDS             37151..38116
                     /gene="lipA"
                     /locus_tag="ACHUT9_001197"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFR1347721.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT         9563 a         9736 c         9578 g         9286 t
ORIGIN      
        1 tgtgtcgaat acgttcggca aattcaaaaa ccaatttgtt ggggtatcgc caagcggtaa
       61 ggcaccggat tctgattccg gcattccgag gttcgaatcc tcgtacccca gccacattaa
      121 aaaagctcgc ttcggcgagt tttttgcttt tctgcgttca ttcaatgtcg aatgcgatgt
      181 tgacacgtct tatccttcaa tgtcggatgc gacgctgccg cgtcttatcc gacctacggt
      241 tggcacgcat ccggcaatgt tgtaaggcta caaccctaac gcatatttca gcgcctgacg
      301 tttcaacacg ccagcacgct ctgccgccat taacccgaga ttacgcacaa aacgcagcgg
      361 cggcagatta ttactgaatc cggcataaaa cagatccata ccactttgca taatgaagtt
      421 atccgccatg cgccgcatct ggtaacgctt gaggacagga taactggccc acgcttcacc
      481 gtagctgcgg gcattcacca gaacatcaat cagggcatcg acatcacgat aaccaagatt
      541 caccccctgc cccgccagcg gatggatggt atgcgcggca tcgcccacca gcgcaagccc
      601 cggctgcaca tactgcaacg catgtcggcg cgtcagcgga aacgcaccag cggcaagcgg
      661 tgtaacgtaa cccagacgcg acgggaaatg cttcgcgatt tccgcctgga attgcgccat
      721 attcatattc tgcaactggc gaatacgcgc cggagagtca taccacacca gcgatgccca
      781 gttatcaaac aacggcagaa acgcacgcgg tccgtccggg gtaaattgct gccaggtgct
      841 gtcgccggga tcgttctcac actgtacgct aatcaacatg cacgactgcg catactgcca
      901 ggcatgaacg ccaattcccg ccatctgccg cacctgagaa tttgcgccgt cggcaccaat
      961 caccagtttc gcgcggatcg tttcaccgcc cttcagttcc agctcctgaa gatcattatc
     1021 tcgatgtaat gcaatcagcg agcctggcac acgtaacgtt actttcggat gcgcttccag
     1081 cgcctgccac agcgcctgtt gcaggacggt gttttccacc atatagccaa gcaacggtag
     1141 cttaagttca gcggcgtcaa acaccacatg cgccgtttcc cactcccacg tttccagtct
     1201 gcggtaagga tggcaacgca tagcctgtac tgcatcccag acccctaacc ctttaagcaa
     1261 tgataccgat gcggcgctga tcgccgagat ccgcacgtcc ggttggctat cagcgacaaa
     1321 cggtgcaggt tctgcgtgct cgatcaccgt taccgaaaat ccgtgctgtg ccagccccag
     1381 cgccagcgcg ccgccgacca ttcctccgcc gacaatggca atttccgttg gttgatttgt
     1441 catggtcaat catcctgtta gcaatatgct ttaagtttac aggattttta cccccaaggg
     1501 ctgataacgc tcatactggt cacaacggca gcaaagcatt acactatacg ccctgcattc
     1561 ctggctacta tttcgcaaga gcaagtcgat gaccaaaaaa ctccatatta aaacctgggg
     1621 ctgtcagatg aacgagtacg attcatcgaa gatggccgat ctgctggatg ccacccacgg
     1681 ctatcaactg accgacgtgg cggaagaagc ggatgtgctg ctgctgaaca cctgctcaat
     1741 ccgcgagaag gctcaggaaa aagtcttcca tcagttgggt cgctggaaac tgttaaaaga
     1801 gaagaatcca gacctgatta tcggcgtcgg tggctgcgtg gcatcgcaag aaggcgagca
     1861 cattcgccag cgcgcccact atgtcgatat tatttttggg ccgcaaacgc tgcaccgtct
     1921 gccggagatg atcaactccg tgcgcggcga ccgcagcccg gttgtagata tcagcttccc
     1981 ggaaatcgag aagtttgacc gtctaccgga accgcgcgcc gaagggccga ccgcgtttgt
     2041 ctccatcatg gaaggctgca ataaatattg cacctactgc gtggtgcctt acacccgtgg
     2101 tgaagaggta agccgtccgt ctgacgatat tctgtttgag attgcccagc ttgcggctca
     2161 gggcgtgcgt gaagtcaacc tgctcggtca gaacgtgaac gcctggcgtg gtgaaaacta
     2221 cgacggctcc accggatcgt ttgccgatct gctgcgtctg gttgctgcga tcgacgggat
     2281 cgatcgtatt cgctttacca ccagccatcc gatcgaattc accgacgata tcatcgaagt
     2341 gtatcgcgac acgccggagc tggtgagctt cctgcatctg ccggtacaaa gcggttccga
     2401 tcgcattctg aacctgatgg gccgtactca cacggcgctg gagtacaaag cgatcatccg
     2461 taaactgcgt gcggcgcgtc cggatattca gatcagttct gacttcattg ttggcttccc
     2521 tggcgaaacc accgaagact tcgagaaaac gatgaagctg attgccgacg tcaatttcga
     2581 catgagctac agctttatct tctctgcacg tccgggtaca ccagccgccg atatggttga
     2641 tgatgttccg gaagaagaga agaagcaacg tctgtatatt ctgcaagaac gtatcaatca
     2701 gcaagcgatg gcatggagcc gccggatgct cggcaccacc cagcgtattc tggtagaagg
     2761 gacttcgcgt aagagcatca tggagctttc cgggcgtacc gaaaataacc gcgtggtcaa
     2821 cttcgagggc acaccggata tgatcggtaa attcgtcgat gtagaaatta ccgacgtcta
     2881 cccgaactct ctgcgcggta aagtggtgcg tactgaagat gaaatgggtc tgcgcgtggc
     2941 agaaacaccg gaatcggtga ttgcccgtac ccgcaaagaa aacgaccttg gcgtgggtta
     3001 ttatcagccg taattctcag gcccgccgtt ccggtgggcc ttgttttttc tctctttatc
     3061 cccatcttta tcgcaatgct tgcgcctttt tcctgtggcg tgaataattt ccttataggc
     3121 cggtgaatgt tcagcttccg gcggcataca gataagagga acggtttgaa catagacact
     3181 cgcgaaatca ccctggagcc agcagacaac gcgcgtctgt tgagcctgtg cggcccgttt
     3241 gatgacaaca tcaagcagct cgaacgccgt ctcggcatcg agatcaatcg ccgcgacaac
     3301 cactttaaac tgaccggccg tccgatttgc gtcaccgctg cggcagacat tctgcgtagc
     3361 ctgtatgtcg atacagcccc gatgcgcggt cagattcagg atatcgaacc ggaacagatc
     3421 caccttgcga ttaaagaagc gcgagtactg gagcaaagtg cggagagcgt gccggagtac
     3481 ggcaaagcgg tcaatatcaa aaccaaacgc ggcgtaatta agccgcgcac gccaaaccag
     3541 gcgcagtaca tcgccaatat tctcgaccat gacattacct tcggcgttgg cccggcgggt
     3601 acgggtaaaa cctacctggc agtggctgcg gcagttgatg ccctggagcg tcaggagatt
     3661 cgccgtattc tgctgactcg tccggcggtc gaagccggtg agaaactggg cttcctgcct
     3721 ggcgatttaa gccagaaagt agacccgtat ctgcgcccac tgtacgacgc gctgtttgaa
     3781 atgctgggct ttgagaaagt tgagaaactg attgagcgca acgttattga agtcgcgccg
     3841 ctggcctata tgcgtggtcg tacgctgaac gacgcgttta tcattctcga tgagagccag
     3901 aacaccacca tcgaacagat gaagatgttc ctgacccgta tcggttttaa ctcaaaagcg
     3961 gttatcaccg gcgacgtcac acagatcgac ctgccgcgta atactaaatc aggcttacgt
     4021 cacgccatcg aagtgctggc cgatgtcgaa gagatcagct ttaacttctt ccacagcgaa
     4081 gacgtggttc gtcaccctgt ggtggcgcgt atcgttaacg cctatgaagc ctgggaagaa
     4141 gccgaacaaa aacgaaaagc ggcgctggcg gcagaacgca agcgcgaaga acaggaacaa
     4201 aaatgagtca ggtgatcctc gatttacaac tggcatgtga agataattcc gggttaccgg
     4261 aagagagcca gtttcaaaca tggctgaatg cggtgatccc gcagtttcag gaagaatcgg
     4321 aagtgactat tcgcgtggtc gataccgccg aaagccacag tctgaatctg acctatcgcg
     4381 gtaaggataa gccgaccaac gtgctctcct tcccgtttga agtgccgcct ggcatggaaa
     4441 tgtcgctact gggcgatctg gttatctgcc gtcaggtggt tgagaaggaa gctcaggagc
     4501 aaggcaaacc actggaggcg cactgggcgc atatggtggt gcacggcagt ctgcatttgt
     4561 taggttacga tcacatcgaa gatgacgaag cagaagaaat ggaagccctc gaaacagaga
     4621 ttatgcttgc tctgggctat gaggatccgt acattgccga gaaagaataa tttgccaggc
     4681 atttgactgg cagacgtccc cgccgttggt gattaacgac ggcgttttga ttaactaaca
     4741 tgagaaccca tacgacgcca tgagcgacga caattcacac agtagtgaca cgataagcaa
     4801 caagaaggga tttttctccc tgttactcag ccaacttttc cacggtgaac cgaaaaaccg
     4861 tgacgaactg ctggcgctga tccgtgattc cgggcagaac gaccttatcg acgaagatac
     4921 gcgcgatatg ctcgaagggg tgatggacat cgcagaccaa cgcgttcgcg acatcatgat
     4981 cccccgctcc cagatgatta ccctgaaacg caaccagacg ctggacgaat gccttgatgt
     5041 catcatcgag tccgcccact cacgtttccc ggtgattagc gaagacaaag atcacattga
     5101 agggattctg atggcgaaag acttgctgcc gtttatgcgc agcgatgctg aagccttcag
     5161 catggacaaa gtgttacgtc aggcggttgt cgttcctgaa agtaagcgcg tagaccggat
     5221 gctgaaagag tttcgctctc agcgttacca catggcgatc gtcattgacg aattcggtgg
     5281 ggtttccggt ctggtgacca ttgaagacat cctggaactg attgttggtg agattgaaga
     5341 cgagtatgac gaagaagatg atatcgactt ccgtcagctg agtcgtcata cctggaccgt
     5401 gcgtgcactg gcttccattg aagacttcaa cgaagcgttc ggcacccact ttagcgatga
     5461 agaggttgac actatcggtg gtctggtgat gcaggcattt gggcatcttc cggcgcgtgg
     5521 cgaaactatc gacatcgacg gttaccagtt caaagtggcg atggccgaca gtcggcgtat
     5581 tattcaggtt catgtcaaaa tcccggatga ctcaccccag ccgaagctgg atgaataaaa
     5641 ccgaaactgg atagataact acatggcttt tgcctcatta attgaacgcc agcgcattcg
     5701 cctgctgctg gcgttattat tcggtgcctg cggaacgctg gccttctctc cttacgacgt
     5761 ctggcctgcg gcgattattt cgttgatggg gcttcaggcg ttgaccttta accgccgtcc
     5821 actccagtct gccgctattg gcttttgctg gggatttggc ctctttggca gcggtattaa
     5881 ctgggtctat gtcagcatcg cgacctttgg cggaatgcct ggcccggtta acatcttcct
     5941 ggtggtgctg ctggcggcgt atttgtcgct gtataccgga ctgtttgctg gcgtgctgtc
     6001 gcgtctgtgg ccgaaaacca cctggctgcg cgtggcgatt gccgcccctg ctctctggca
     6061 agtgaccgag tttctgcgcg gttgggtact gacaggcttc ccgtggttac agttcggcta
     6121 tagccagatt gatggcccgt taaaagggct ggcaccgata atgggcgtgg aagccattaa
     6181 cttcctgctg atgatggtta gtggcctgct ggcactggcg ttggtcaaac gcaactggcg
     6241 tccgctggtg gtggccgtcg tgctgtttgc cctacccttc ccgctgcgtt acatccaatg
     6301 gtttaccccg caaccggaga aaaccattca ggtttcgatg gttcagggcg atattccgca
     6361 atcactgaaa tgggatggag atcagcttct taatacgctg aagatttact acaacgcaac
     6421 ggctccgctg atgggcaaat catcgttgat tatctggccg gagtcggcga taaccgatct
     6481 ggaaattaat cagcaaccgt tcctcaaagc actggacggt gagttgcgcg ataaaggtag
     6541 ctcgctggta accgggattg tcgacgcgcg tctcaataag cagaaccgct acgataccta
     6601 caacaccatc atcacactgg gtaaaggtgc accatacagc tacgaatcag ccgatcgcta
     6661 taacaaaaac catctagtgc cgtttggcga gtttgtcccg ctggagtcga ttctgcgtcc
     6721 gttagcaccg ttctttgatc tgccgatgtc gtcgttcagc cgtgggccat atatccagcc
     6781 gccgctgtcg gtaaatggta ttgagcttac tgcggctatt tgctacgaga tcattctcgg
     6841 cgagcaagtg cgcgataact tccgcccgga taccgactat ctgctgacta tctccaacga
     6901 tgcgtggttt ggtaagtcta ttggtccatg gcagcacttc cagatggcgc gaatgcgtgc
     6961 gctggagctg gcgcgcccac tgttgcgcag caccaacaac ggcattacgg cggtgattgg
     7021 cccgcagggg gaggttcagg cgatgatccc gcagttcacc cgcgaggtgt tgaccactaa
     7081 tgtgacaccg accaccggac tcacaccgta tgcacgtacc ggcaactggc cactgtgggt
     7141 gctgacggcg ctgtttggtt ttgccgctgt gttgatgagt ctgcgagcac gtaaacgttg
     7201 atgtgcctga tgtatcctgc atgagcaaaa cgtactttct cattgagctt tggggcatgg
     7261 ttatcatgcc cctttttctt acggttcagg gatacaagcc acattgccat attggggatg
     7321 acgaataatt gcctgcggat caagcgagcg cgtcctgtcc atttggatca acccgttctt
     7381 cgccatgaca aatatttttc taccatccgc tgaaaaacga aggtgattaa cgtagctggc
     7441 aggataaacg ccaataatct gcccggcatt aatattgagc aggatgacat aaccttgcag
     7501 gccatatgac accaacgcta aatattgacc gtccgggcta ataccctcaa cggaataaac
     7561 gctcctgcta caccattgcg tcataatgct ggcttgttga tcccctacgc ttatacgtga
     7621 aatcccctga ctatcggcaa aaataacctc gccgttttca ggtcgaaaac gcatcgccga
     7681 tatcgttcca cgcgcatcac ctgcgggaaa gtcttctttc tgatgcgtga tggtattgat
     7741 ccgcacgatg ttacctctgc ctgctcccgg aaccagtaat gttctttcat ccggggaaag
     7801 tgccataacc caggcgcctg gtttaccgct cgtccccgtc ccgtcgtatt tctccagcgg
     7861 gatatccgcc acatgctgat aagtcaccag atcaatcgct gacacactat tactggtgcg
     7921 attcatcacc caggcttttg tgccatcacg cgagaggata aggctgtcag gatgtgcacc
     7981 ggtggtaatc gactgaataa tctcccctgt cgccacgtta atgaccgata acgcattcgc
     8041 ttcaaatgaa gtcgcataaa tcaacttttc atccgggctt aatgccagcg atctgacgcc
     8101 ctgatcttta cagccaccac aatgtttgac tgacttttct ttcgctatgg gcgctgtaaa
     8161 ggtatgcagg attttctttt gtccgaggtc gaaaacaaca atgctgttcg tgttttcaat
     8221 tgcggcatac aacatattac catcccgact gatcgccata tcatggatat cggcaatctc
     8281 agcgagcaaa tcaccactgg cctgttcacg acttaccgga gcggataatg tttccctgag
     8341 ggcgttgcgt tcctccgcta actgctgctt tttttgccgt cgctcttgtt caatctcctg
     8401 tggataaaac gcttcacgca ggctttgcct gacctgtaaa actgactcat ccatctgctg
     8461 ctgcgcaagc cagatagccc cggtcagaag aagactgccc gcaacaataa tcccgccttc
     8521 cgtaacgagt cggttttgcc cagataaacg cgcgctcagc caacccaacg cgccacccgc
     8581 aatcaggccg ccaatgtggc aagcgttatc tattcctgtt tgccgggcac cgtacaacag
     8641 tgtcagcgcc accataccca acagaggaaa tactcgccgc tggtttttat ggtatgtgcc
     8701 cgtacctgaa gcaaactgtg ttgctatcgc tgcaccagct attcccagaa tcgccccgga
     8761 tgccccaacg ctgatgacta tctgctcact ttcgcgtaac gcataacagg cgctgatgag
     8821 cccaccaaag acgccggaga ataaccaggt aatcaggagt cgccatttcc ccagaatctg
     8881 ttccgccacg cctcccacga ccaggaatgc aaacatattc atcagcagat gagaaacact
     8941 actgtgcaga aagatactgc tcaataagcg ccagggttca cccgacaaag taagaggcgc
     9001 aacgtttgcg ccgagatgga taagtgcgga ttgatcgacc tcttcataac tcatccattg
     9061 tgcgagataa caaaatatcc caatacagag tgcaaaaagg ataagggtta ttgcgggtaa
     9121 ttgaacattt aacggcttaa ccgaagatgc agacataagg tgtttcttcc ttgaaaagac
     9181 aggtaaaaag catttttccc gtgtgcagtt atcatcccgg caaacgtccc atgccgaaaa
     9241 gataattctg tccgaaatat gactttttca gcctaaaatt acaatcgtaa ttaattcgcc
     9301 cctaagtatt ttttggcatg taagttgctt tatttactgt gtaagcgatg aaatcagccg
     9361 cgacgcaacc aggttgcacc accacaaatc agcggtagca ccaaaaaggt gcggtttaag
     9421 aattttgctt cggaatggtg cggcgcatct cgcaaaaaca aacaataaaa cagcaatatc
     9481 tttacattta gccaaactaa atgttaccta acaggcacaa cactgcacaa taaagttgca
     9541 gacgataaca acacaaacac tcacaacggg tatccatgcg ttcttaacgc agaagataaa
     9601 ggagttggat atgcaattac gtaaacctgc cacagcaatc ctcgccctgg cgctttccgc
     9661 aggactggca caggcagatg acgccgcccc ggcagcaggc agtacgctgg acaaaatcgc
     9721 caaaaacggt gtgattgtcg tcggtcaccg tgaatcttca gtacctttct cttattacga
     9781 caatcagcaa aaagtggtgg gttactcgca ggattactcc aacgccattg ttgaagcagt
     9841 gaaaaagaaa ctcaacaaac cggacttgca ggtaaaactg attccgatta cctcacaaaa
     9901 ccgtattcca ctgctgcaaa acggcacttt cgattttgaa tgtggttcta ccaccaacaa
     9961 cgtcgaacgc caaaaacagg cggctttctc tgatactatt ttcgtggtcg gtacgcgcct
    10021 gctgaccaaa aagggcggcg atatcaaaga ttttgccgac ctgaaagaca aagccgtagt
    10081 cgtcacttcc ggcactacct ctgaagtttt gctcaacaaa ctgaatgaag agcaaaaaat
    10141 gaatatgcgc atcatcagcg ccaaagatca cagtgactct ttccgcaccc tggaaagcgg
    10201 tcgtgccgtt gcctttatga tggatgatgc cctgctggcc ggtgaacgtg cgaaagcgaa
    10261 gaaaccagac aactgggaaa tcgtcggcaa gccgcagtct caggaagcct acggttgcat
    10321 gttgcgtaaa gacgatccgc agttcaaaaa gctgatggat gacaccaccg cgcaggtgca
    10381 gacctccggt gaagcggaaa aatggtttga taaatggttc aaaaatccca ttccgccgaa
    10441 aaacctgaac atgaatttcg aactgtcaga cgaaatgaaa gcactgttca aagaaccgaa
    10501 tgacaaggca ctgaactaat tacaagaacc aggggcggaa attccagccc tctcgattgt
    10561 tacgtagcac ggacagacta tacgcctgat ggtcgttccc catcgggcct gaaaaccgca
    10621 atacgctggg taacaatctt cgagggtagc agttaacgct gctaccctct tttttctgga
    10681 gtagatttat gtctatagac tggaactggg gtattttttt acaacaagcc ccgttcggca
    10741 acaccaccta tcttggttgg atctggagtg gttttcaggt gacgatcgct ttgtcgatct
    10801 gcgcctggat tatcgctttc ctcgtcggtt catttttcgg cattttacgt accgttccaa
    10861 accgttttct ctccggtctg ggtacgttgt atgtcgaact gttccgcaac gtgccgttga
    10921 ttgtgcaatt ctttacctgg tatctggtga tcccggagct gttgccggag aaaatcggca
    10981 tgtggtttaa ggccgagctg gatcccaata ttcagttttt cctttcatcc atgctctgcc
    11041 tggggctgtt taccgccgcc cgtgtttgcg aacaggtacg cgccgccatt cagtcgttgc
    11101 cgcgtgggca aaaaaatgcc gcgctggcga tgggcctgac gctaccgcag gcttatcgat
    11161 acgtactgct gcctaatgct tatcgcgtta tcgtcccgcc gatgacttcc gagatgatga
    11221 acctggtgaa aaactccgcc atcgcctcga ctattggtct ggtggatatg gcggcgcagg
    11281 cgggtaaatt gcttgattac tcagcccacg cctgggagtc gtttaccgct attacgctgg
    11341 cgtatgtgtt gattaacgcg gttatcatgc tggtaatgac gctggttgaa cgtaaagttc
    11401 gcctgcctgg caacatgggg ggcaaataat gtacgagttt gactggagtt ccattgtccc
    11461 ttccctgcca tatctgctcg acgggctggt gatcacgctg aaaatcaccg tcacggcagt
    11521 cgtgattggt attttgtggg gcaccatgct ggcggtgatg cgcttgtcca gctttgcgcc
    11581 cgtagcgtgg tttgccaaag cctacgttaa cgtattccgc tcgattcctt tagttatggt
    11641 tttgctgtgg ttttacctga tcgtgccagg ttttctgcaa aacgtgctgg gattatcacc
    11701 aaaaaatgat atccgtttaa tttcggcgat ggtggcgttt tcgatgtttg aagcggccta
    11761 ctattcagag attatccgtg ctggtattca aagtatttct cgtggtcagt cgagcgccgc
    11821 gctggcgttg ggaatgactc actggcagtc gatgaaactg attattctgc cgcaggcgtt
    11881 ccgcgcgatg gtgccgcttc tgctcactca gggcatcgta ctgttccagg atacctcact
    11941 ggtgtatgtg ttatctctgg ccgatttctt ccgtaccgcc tcaaccattg gtgagcgtga
    12001 tggtacgcag gttgagatga tcctgtttgc cggatttgtt tatttcgtta ttagtcttag
    12061 cgcgtcgttg ttggtcagct acttgaaaag aaggacagca taatgattac cctgaaaaat
    12121 gtttcaaaat ggtatggtca ctttcaggtg ctgaccgact gctcaaccga agtgaaaaaa
    12181 ggcgaagtgg tggtggtttg cggcccgtct ggttccggca aatcaacgct gattaaaacc
    12241 gtcaacggcc tcgaaccagt gcagcaaggt gaaatcaccg tcgatggtat cgtggttaat
    12301 gacaagaaaa ccgatctggc aaagctgcgt tcccgcgtcg ggatggtatt ccagcatttc
    12361 gagctgttcc ctcatctgtc gattatcgaa aacctgaccc tggcgcaggt gaaagtgctt
    12421 aaacgcgata aagcgcccgc tcgtgaaaaa gcattgaaat tgctggagcg cgtgggactt
    12481 tccgcacatg ccaataaatt tccggcgcag ctttccggcg gtcagcagca gcgtgtggca
    12541 atcgctcgcg cattgtgtat ggatcctatt gcgatgctgt tcgacgaacc gacatcggcg
    12601 ctggatccgg agatgatcaa cgaagtgctg gacgtgatgg tggaactggc gaacgaagga
    12661 atgaccatga tggtggtgac ccacgaaatg ggctttgccc gcaaagtggc gaatcgggtg
    12721 atctttatgg acgagggtaa aattgtcgaa gactcgccga aagacgcttt cttcgatgat
    12781 ccgaaatcgg accgcgcgaa agacttcctc gcgaaaatcc tgcattaatc actctggcgc
    12841 gcatacccaa gtgcgcgcca tctcacgctt tatcgtttcg cccctcgtat ctgttacatc
    12901 ctggcgttac ccttgtcgca aagaagcaca caacaaggag caacaatggc actgccaatt
    12961 ctgttagatt gcgacccagg tcatgacgac gctatcgcaa tagttctcgc cctcgcctca
    13021 ccagagcttg atgtcaaagc aattacgtct tccgccggaa accagacacc agaaaaaacc
    13081 ttacgcaatg ttctgcgtat gctgaccttg cttaatcgca ccgatattcc ggtagcaggc
    13141 ggcgcggtaa aaccgttaat gcgtgagttg attatcgcgg acaatgtgca cggcgaaagc
    13201 ggtctggatg gcccggcatt accggaaccg acgttcgcac cgcaaaactg tacggcggta
    13261 gagctgatgg cgaaaacgct gcgcgaaagt gcggaacctg tcacgattgt gtctaccgga
    13321 ccgcaaacta acgttgcctt gctgctcaat agccatccgg aactgcatag caaaattgcc
    13381 cgtatcgtga ttatgggcgg cgcaatgggg cttggtaact ggacgcctgc ggctgaattt
    13441 aatatttacg ttgacccgga agcggcagaa attgtcttcc agtcagggat cccagtggtg
    13501 atggccggtc tggatgttac tcacaaagca caaatccacg ttgaagacac cgagcgtttc
    13561 cgcgcgattg gcaaccctgt ttcaaccatt gttgccgaac tgctggattt cttcctcgaa
    13621 tatcataaag acgaaaaatg gggctttgtc ggtgcaccgc tgcatgaccc atgcaccatc
    13681 gcctggctgt tgaaacccga gttgtttacc actgttgagc gctgggttgg cgtggaaaca
    13741 caggggaaat atagccaggg tatgacggtt gttgattatt attatctgac tggcaataaa
    13801 ccgaatgcca ctgtaatggt cgatgttgat cgtcagggct ttgttgattt actggctgat
    13861 cgtctgaaat tttacgctta acacacgtca ttgattaaga tcaccacgcg ttacctgggt
    13921 ggtcttaatc tgtttatctg tttatctctt tttctttcaa gatataaatg caatggataa
    13981 tgcagaactc gccattggta tcgatctcgg cactaccaat agtttaattg ccgtctggaa
    14041 agacggtgcc gcgcaattaa ttccaaataa gttcggtgaa tatttaacac catccataat
    14101 tagcatggat gaaaataatc atattttagt cggaaaaccg gctgtatcac ggcgtacttc
    14161 gcatccggat aaaacggcag cgttatttaa acgtgcaatg ggcagtaata ccaactggcg
    14221 gttaggcagc gacacattta acgcgccaga actgtcctct ttggtattac gctcattaaa
    14281 agaagatgcc gaagaatttc tgcaacgtcc gattaaagat gtggtgatct ccgttccggc
    14341 ttatttcagc gatgaacaac gcaagcatac ccgtttagca gcggagttag ccgggttaaa
    14401 tgcggtacgc ttaattaatg aacccacagc agctgcgatg gcatatggcc tgcatacgca
    14461 acaaaatacc cgctcgctgg tttttgatct cggtggcggc acgtttgacg tcacggtgct
    14521 cgaatacgcc acgccggtga ttgaagttca cgcctccgct ggcgataact tcctcggtgg
    14581 cgaagatttt acccatatgt tggtcgatga cgttttaaag cgcgcggatg tcgccaggac
    14641 cacgcttaac gagagtgaac tggcagcctt gtacgcctgt gtggaagcgg caaaatgtag
    14701 caatcaatcg ccattgcaca ttcgctggca gtaccaggat gaaacgcggg agtgcgaatt
    14761 ttacgagaac gaactggaag atttgtggtt gccgctgctc aatcgcttgc gagtgccgat
    14821 tgaacaggcg ttgcgcgatg cacgtctgaa gccgagtcaa atcgacagtc tggtgctggt
    14881 tggcggcgcg tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt
    14941 accgtatcaa agttacgacc cgagcaccat tgtcgcgctg ggcgcagcaa tccaggccgc
    15001 ctgccgctta cgcagtgaag atattgaaga ggtgatcctc actgatattt gcccttactc
    15061 gttgggcgtt gaagttaacc gccagggcgt ttccggcatt ttctcgccaa ttattgaacg
    15121 aaacaccaca gtacccgtgt cgcgtgtaga aacttactcg accatgcacc cggaacagga
    15181 ttctattacg gttaacgtct atcagggaga aaaccacaaa gttaaaaata acattctggt
    15241 tgaatccttc gatgtgccgt tgaagaaaac cggggcttat cagtcgattg atattcgctt
    15301 tagttatgat atcaacggct tgcttgaagt tgacgtgctt ctggaagatg gcagcgttaa
    15361 gtccagagtc attaatcata gcccggtaac attgagcgcg cagcagattg aagagagtcg
    15421 gacgcggtta tccgcattga aaatttatcc gcgcgatatg ctaatcaacc gcacctttaa
    15481 ggccaaactg gaagagttgt gggcgcgggc gctgggtgac gagcgagaag agattggccg
    15541 ggtgatcacc gattttgatg cggcgttgca gtcaaacgat atggcccgcg tcgatgaagt
    15601 tcgtcgacgg gcgagcgatt atttggctat tgagatccct taaacacggc tgccggatgc
    15661 ggcataaacg ctgcatccgg catcggcgca aaaaacacat cctcagctaa acaacatcgc
    15721 gatttttccg agcgcataac ctatcatcag caccgttaag ctgattttga taatactggt
    15781 aatgctctgt ggtttttcat cctcatcatt tttttcttca tggttttcag gtgttggctc
    15841 atcgacatct tcatcctccg gctcgagttt cactttcgtc aacagcatct gggtgataat
    15901 ggcctctaat ggatgagcat ctccaccttc cagccttgta ttaagacggg ccagcatctc
    15961 ttgtttcggg aacgcttcca gcacaacctg ttccgctacc cgatacaaat ccagtaacgg
    16021 tgaatcttgc tctgtctcgc taccgactct cgatttccac cacgcccatg ctttgcgctt
    16081 gaagaataac gactcaataa acgattgacc taacggcgat aattttccgc tatgtaaaat
    16141 ctccgcccat aacagccgcg tccgggcgct ctcaccagga acatatttca acggtttacc
    16201 ttgcgcatct accatgcttc gcgcttcgat aaccatcact aataacgcga aataatccgg
    16261 gcaatgacgc ttgctccact gcaacaacaa attatcagct tgtgtagatg ggtaactttc
    16321 ccagtacgcg cagagatcgg caagcaggct ttccccctcg ccgcagtaca aacgttgtgc
    16381 acactggtaa taccgggcat cttcgctttc tggttcttcc gcttgccact gtttggctac
    16441 aggaatgagt tccgccatac ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg
    16501 taactttgca tcatcaggaa tgaaccacgg tccttccatc gccaaaaacg cagtgacata
    16561 gtcaggatgg taacggcagc aacgttccag cgcgaagtaa tattcaacag tctggttctg
    16621 taacgcgacg ggatattcca gaaggctaag gaaatcgaaa caatcaccag cctgaatggc
    16681 ctcaagaaaa gcctcccttt cttcctcatc cattccttcg ctgtcattaa acgaatgcca
    16741 gttcaaacgc tctgccaaca aagaagcaca atttaatgaa agatatcgcg cttctatagc
    16801 gattgcacac agcggccagc gtaattgatc gacatcctcc atgttccagg tatttaattg
    16861 ctgaataaat ttctgccagg cggagggctg aaaacgatca ctttctgaat ccagtaatgt
    16921 cctgaatgca cgtagtattt catgttcagc ggtggcatct ttttcttcat catctgcttc
    16981 ctcgacagga tttaccgcca gtcgcagcgc ctcttcatag gcctggcgta atgctttaaa
    17041 cccctgcgga tcggtttcgg gatgacacaa cggtaagcga gcaagataag cctggcggat
    17101 aatgtctatt tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt tcataggtcg
    17161 ctcacatcct taagcgttac cgggcattca ggctggacag ttaatggttg ccagggaagc
    17221 gtggcataga aacggtcgct tcccgctgcc agctcttcga acatccgact cccggcgcta
    17281 tcgtatttgt acagggttag cgcctcgcgt agcgaagcgt tgtcttcgca ctgagaagat
    17341 tgccagtaga gacgccctag tgaataggca gaaaaatagt gcatccagct gtgatagtaa
    17401 tggtgagctc gtgcatagac gcgcgattgc agccataaac tctcttcttc cgtcagccag
    17461 ttgttgagca caccgacccg tgacagaaac cccattcgaa cataatccca ggctttgatt
    17521 cccccttctc cacagcacat agcggtttgg gcaacgaatt gggtataaat caaccctctc
    17581 tccgagccac cagcaattaa tgctttccac tcttcagggg aagagcgttg ccatgtcaga
    17641 tataagccag ctaaatcatt ggcatgacca tcatcagtca tctgataaat agtctgaatt
    17701 aaatcatcac gattatttat tccccaggat gatgagagat cgaattcttt tatcccagta
    17761 tacatttttg gcgagtcatg cgcgggtttg caactctgat taagtatttc catgggcgca
    17821 gaaagcgcaa aaagaagata ttgttcttcc ttattcattt tttaataatc ccttaagtgc
    17881 attaactgac tactttttta ttattgatga gtataaactt gacgctttca aaataaaaag
    17941 aaaatcgaag cattcacaca tgaataaaaa atcaatgtat atattcgcaa tttttatagt
    18001 tgcagcaatt acctgtatta gccaacccaa gaaaacgacg ttgcgtgata aagccatggt
    18061 gaattatgcc tttgattatt taagatcacc aggcagtcta ccgttaacca cggcagccac
    18121 ggagctttcc gcgattcatg gtcactcaac atcgcaatat cgccttggcg aattttatct
    18181 tcatggtagc gacggtaaac cactggatta tacacaggcg agatactggt atgagcaatc
    18241 agcagaacag gaaaatccgc gcgcacaaag caaactgggg tggatctacc tgaaaggcct
    18301 gggagtcaaa cccgataccc gtaaagcaat tctctggtat aaggaagcag ctgaacaagg
    18361 gtatgctcat gctcaatata ctttgggttt gatctacaga aatggcactg gtattaatgt
    18421 taaccattat gaatctcaaa aatggttaaa actggccgcc aaacaacatt acaaaaatgc
    18481 ggaaagatta cttgccggac ttcccgcaca ttaacgtcga ctgtcacccg aagagatgga
    18541 gtatttttcc tatcaaaccg gcaaagataa aaatgtagaa aataattttg atgacgccca
    18601 gacagcccag cccgccatcg tcaacgggca ttggctgcgg ttgcggctcc ggctcgacgg
    18661 tttcctgacc ggtaaaattc gccgcctgaa ttaataatcg acgaataatt aatgcttcca
    18721 gtggacaacg atcttctgca tcaaggcggc tcacaatcgg cattaatgat tcttctgtcg
    18781 gttggctggc ctgcactaat tgtttggcaa ctaaatataa tggccataag ggatgttctg
    18841 tatcacgcgt gagtgagatc cgcggcttac gtcctttgtg caaaaccatc tcgaccagtg
    18901 aacgccccag cgcagagagt ttttcatcgc tgagtatttc gtataaatga aaacgcgtac
    18961 gggcgctgtc gccggggaca taaagtagct gtttaccctg atcgttaacc agttcacgag
    19021 cttcaatcat catcaccact aacgggtaat aatcgacccg atgctgacgg caccattgca
    19081 atattaaagc atccgcctgg gtggagggat actcgcgcca gtagtcacaa agttctgcca
    19141 gcaagctttc gccttctcca caatagacgc gttgcgcgta ttcataataa cgcgcatctt
    19201 cattatcagg ttcagcttcc cgccacgacc gggcgtaatc cagaagttcg gggatatcca
    19261 ggcgacctgc gctataccag cggagtaaat ttctgtgcag ttttgcatca tcggggatga
    19321 tcactgcgcg atgttgcgct aaatacaatg tcagccattc tggatgcgat gaccaaatcc
    19381 gagcgaacat ttggtaaaaa tcgatggtgt cattttgcac ggcaacgggc agatgcagaa
    19441 tggtctggaa gttaaaaaca ttccccttcg caatggcata taaaaaggat tccagttctt
    19501 cttcgtctat ttccccgacg ttttcctcct gcaaccaccg caatctttct gctaataaca
    19561 ccacgcactc gaaggataaa tgggcagtgt tcatggcgat tgtgcacagc gaccagcgta
    19621 attcatcaat ctcttccatc gagcaataat ttaattgctg aatgaatcgc tgccaggcgg
    19681 agggcagaaa acgttcgcta tcagaggcaa gtaacgcgcg aaaggcgagc agaatttcat
    19741 gttctgctac ctcatattct tctggttgcc aaacagattt agcaggcgac tgcgcaatcc
    19801 ggagcgcctc ctcatacgcc tgacgaagtt gtttaaaacc ctgcggatcg gtttctggat
    19861 gaaaggacgg taacagcgcc agataagcgc ggcggataat atcgacgtca gtcgtttcct
    19921 ctatttcgag gatcttccag caatttttca taggtcgctc atatccttaa gcgtttccgg
    19981 gcatgtgggg tagtcagcca gtggttgcca ggacaaggca gggtaatacg catcatcact
    20041 ggcaaataat tgataaaaca tatcattgcc ggaatcgtcg tattctttac gagctaagaa
    20101 ttcgcgtaat aaaggcagat gatcatcttc gggagactgc caatattgcc gaccaaaggt
    20161 ataaccggca aagtattgcc gccagttact ataataacgt agagcccgta gatgaatacg
    20221 cgactggatc catagacttt cttcttcact caaccagtta ttaagtacgc ccattcgact
    20281 taaaaaaccc atccggacat aatcccatgc tttaattcct ccctcaccac agcattcggc
    20341 tgtgctggcg acaaattgcg cataagcctg accttgctca tttagttcag caagatagtc
    20401 acgccattca catggcgaat aacgaaacca gcggtgataa aaccctgcca accgagcggc
    20461 atgaccgtta tcgatcattc ggtgaattgt gcgcagcagg tcatcacggt catcaattcc
    20521 ccatgaggaa gaaaggtcaa aaaagcatgt acctaaatac attttggggg atgaatgcga
    20581 aggtaaacag cattcattgc gtatttccat cggagtggaa agtgcaaaca atattttttg
    20641 cgaccccatg tccatataat ttatccttaa acattcattt aatgaatgta aactacattt
    20701 cgagactcag ttatttatac ttttctgacg caacataata cgcataaaat aaggcttacg
    20761 caaaaatgga acacataaaa atataatatg ttttatctac aaccataagg aaatgataat
    20821 ggtttttacg tcaagttgct gcgataattt atcaatagat gagattatcg aacgtgctga
    20881 aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg atagcgcaat
    20941 tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgctg agcaaggtca
    21001 ttgtgaagca cagtattcct tagggcaaaa gtataccgag gataaaagcc gtcataaaga
    21061 taatgagcaa gccatcttct ggctgaaaaa agctgcccta caaggccata ctttcgcttc
    21121 caacgccctt ggctggacac tggatcgtgg agaagccccc aattataaag aagcggttgt
    21181 ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc ttgggtggat
    21241 gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct ggtacaaaca
    21301 agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt atgaagacgg
    21361 aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa gcgcacagca
    21421 gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg aaggggtgga
    21481 ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg aaagcgtcgg
    21541 cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga aagattatca
    21601 ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg cctggtataa
    21661 cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac aggctctcga
    21721 cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa ttcgtgagac
    21781 tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc ccatattctc
    21841 tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg aactggtcat
    21901 gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga tggaagtgac
    21961 atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa accccatgat
    22021 gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg atatctgcca
    22081 gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat tcttctttca
    22141 ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct cgcgtcagct
    22201 catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc gcctgttcca
    22261 ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac gcgaccagtt
    22321 cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta actgactata
    22381 ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg taaattaatg
    22441 tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt tgacgcggac
    22501 gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg tagccgtatt
    22561 gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga tagaatccaa
    22621 agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg agagcaaaga
    22681 gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca tgggccacgt
    22741 acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg gcaaaaacgt
    22801 cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg cggtgaaaaa
    22861 caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa accagctcaa
    22921 aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc cggaatacta
    22981 ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat ataagaagac
    23041 ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac aagttatcga
    23101 cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc agtggtttat
    23161 caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg atcactggcc
    23221 agacaccgtt aaaaccatgc agcgtaactg gatcggtcgt tccgaaggcg tggagatcac
    23281 cttcaacgtt aacgactatg acaacatgct gaccgtttac actacccgcc cggacacctt
    23341 tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga aagcggcgga
    23401 aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag ttgccgaagc
    23461 tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg ttcacccatt
    23521 aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt acggcacggg
    23581 cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct ctaaatacgg
    23641 cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc tttctcagca
    23701 agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc ttgaccatga
    23761 agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg agcgtaaagt
    23821 gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg cgccgattcc
    23881 gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc tgccggtgat
    23941 cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag atccggagtg
    24001 ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt tcgacacctt
    24061 tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag aaggtatgct
    24121 ggattccgaa gcggctaact actggctgcc ggtggatatc tacattggtg gtatcgaaca
    24181 cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg atgcaggcat
    24241 ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc tggcagatgc
    24301 cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg atgctatcgt
    24361 tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc atgaactggt
    24421 ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc cgcaggtgat
    24481 ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt ctccggctga
    24541 tatgactctc gaatggcagg aatccggtgt ggaaggggct aaccgcttcc tgaaacgtgt
    24601 ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga acgttgatgc
    24661 gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg ctaaagtgac
    24721 cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta tggagctgat
    24781 gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga tgcaggaagc
    24841 gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct tcacgctgtg
    24901 gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg ctgacgaaaa
    24961 agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag tccgtgccaa
    25021 aatcaccgtt ctggtggacg caacggaaga acaggttcgc gaacgtgctg gccaggaaca
    25081 tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg taccaggtaa
    25141 actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct ggcaacattg
    25201 ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg tgataccacg
    25261 caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa cgggccatta
    25321 agccgtgcag tgcgtaacca gttacgtctg aatggtgtcg agttgcttga taaagaaacc
    25381 acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa agataccgca
    25441 tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt gaatgcgacc
    25501 gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg ttcgttcttt
    25561 gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt aaaagagatg
    25621 tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc tgcggatatt
    25681 cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc tgcacgcgtc
    25741 tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc gcgcagctca
    25801 atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta ttgcaggaaa
    25861 gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac cacacttttt
    25921 ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct atgagtctgt
    25981 ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat gcggcgatca
    26041 atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg atcgtccgcg
    26101 gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgctctt gcgaatcgca
    26161 gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg gttgctgcgc
    26221 gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc tgctactgtt
    26281 atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg ctctggccag
    26341 acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg catttcaccc
    26401 cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcgcattg catattcttc
    26461 agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta caacgtgaac
    26521 tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt gcgttgtttg
    26581 ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta aatcgcttaa
    26641 gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc accctcaaac
    26701 aagattacgg tcagtcagtg tgggcagagc tgaaagggtt atctcttctg ttgtgccata
    26761 aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc tctgtttggc
    26821 ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct ggcgaatttg
    26881 attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg tccccagccg
    26941 gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga caagccatta
    27001 tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc gcaaacactg
    27061 aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat tggtcaggat
    27121 tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa tgcacatttg
    27181 atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata ccagcaatgg
    27241 ctggaagatc atttgacaca taacccggaa gatcttcacc ttcagcctgc cggtaaaatt
    27301 tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga acgtttgcaa
    27361 aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat taaccaacaa
    27421 ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg ttaattcgtc
    27481 atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc acccccctga
    27541 ccgcgcgcgg tattgagcaa gcgcaaaatc tgcatacgct gctacatggt gtttcctttg
    27601 atctggtttt atgcagtgaa ctggaacggg cacagcatac cgcacgactg gttctcagtg
    27661 accgccagct ccccgtgcaa atcatccctg aactcaacga aatgtttttt ggcgactggg
    27721 agatgcgaca tcatcgcgac ctcatgcaag aagatgccga aaactatagc gcgtggtgca
    27781 atgactggca gcatgcaatc cccacgaacg gtgaaggatt tcaggcattt tcgcaacgtg
    27841 tggaacgctt tatcgcaagg cttagtgaat ttcagcacta tcagaatatt ttagtcgtca
    27901 gccatcaggg tgtactgagt ctgttaatcg cccgtttaat tggcatgcct gccgaggcta
    27961 tgtggcattt tcgcgttgac taggggtgtt ggagcgccat tgatatcaac caaaaattcg
    28021 cgacgctacg cgtcctcaat agccgtgcca tcggggtcga gaatgcatga cttttctgtt
    28081 tttttacggg taagccgcaa cgaccattga cagacccggg caggctgata ttctccgcag
    28141 ccagactttt ttcgccagac acgactttgt agaaattgtt ttacaaaaat ggcgatgcaa
    28201 tctgcggcgc ggggtgggat gatagcccac tttcgaaagc cgattcggcg acaattgtcc
    28261 cgaaatcgcc tctggttcag gtatactgac agaccatttt tatctatttg attcacccag
    28321 ggggaaaact tgcagggtaa agcactccag gattttgtta tcgacaaaat tgatgacctc
    28381 aaaggtcagg acatcatcgc cttagacgtt caaggcaaat ccagcatcac cgactgcatg
    28441 atcatctgta cgggtacgtc cagccgtcat gttatgtcca ttgctgacca cgttgtgcag
    28501 gagtctcgcg cagcgggcct gttaccgctc ggcgtagaag gtgaaaacag cgccgactgg
    28561 attgtcgtgg atttgggcga tgtgattgtc catgtcatgc aggaagagag ccgtcgcctg
    28621 tatgaactgg aaaaactctg gagttaatgc gtgaagctgc aacttgtcgc cgtgggaacg
    28681 aaaatgccgg actgggtaca aaccggtttt accgagtacc tgcgtcgttt tccgaaagat
    28741 atgcccttcg agctgattga aattccggcc ggaaaacgcg gcaagaatgc ggacatcaag
    28801 cgcatactcg acaaagaggg tgagcagatg ttggcggccg caggcaaaaa ccgcattgtc
    28861 accctcgata ttccaggcaa gccctgggat acgccgcagt tagccgctga gctggaacgc
    28921 tggaagctgg atggtcgcga cgtcagtcta ctgattggcg ggcctgaagg gttgtcgcct
    28981 gcctgtaaag cggcggcgga gcagagctgg tcgctgtcgg cgcttaccct cccccatccg
    29041 ctggttcgcg tgctggtcgc agagagtctg taccgggcgt ggagcatcac caccaaccat
    29101 ccttatcacc gtgagtgata agggagcttt gagtagaaaa cgcagcggat gaaactacag
    29161 aactcttttc gcgactatac ggcagagtcc gcgctgtttg tgcgccgggc gctggtcgcc
    29221 tttttgggga ttttgctgct gaccggcgtg cttatcgcca acctgtataa tctgcaaatt
    29281 gttcgcttta ccgactacca gactcgctct aatgaaaacc gcattaagct ggtgcctatc
    29341 gcgcccagcc gcggcattat ctacgaccgt aatggtatcc ctctggccct caaccgcact
    29401 atctaccaga tagaaatgat gccagagaaa gtcgataacg tgcagcaaac gctggacgct
    29461 ttgcgcagcg tggttgatct gaccgatgac gatattgctg cattccgaaa agagcgcgca
    29521 cgttcacacc gtttcacctc tattccggtg aaaactaacc tgaccgaagt acaagtagct
    29581 cgctttgccg tcaatcagta ccgttttccg ggtgtcgaag ttaaaggcta taaacgtcgt
    29641 tactatcctt acggttcggc gttgacccac gtcatcggct atgtgtcgaa aatcaacgat
    29701 aaagacgtcg aacgcctgaa taatgacggc aaactggcca actatgcggc aacgcatgat
    29761 atcggtaagc tgggcattga gcgttactat gaagacgtgc tgcacggtca gaccggttat
    29821 gaagaggttg aagttaacaa ccgtgggcgt gttatccgcc agttaaaaga agtaccaccg
    29881 caagccggac acgatattta cctgacgctg gatctcaaac tccagcaata tattgaaacg
    29941 ctgctggcgg gtagccgcgc agctgtggtt gtcaccgatc cgcgtacagg tggggtgctg
    30001 gcgctggttt ccacgcctag ttatgaccca aacctgtttg ttgacggtat ctccagcaaa
    30061 gattattccg ccttgttgaa cgccccgaat acaccgctgg tgaaccgcgc cacacagggg
    30121 gtttatcctc ccgcgtctac agttaaaccc tatgtggcgg tttcggcatt gagcgccggg
    30181 gtgatcacgc gcaatacgac gctgtttgac ccaggctggt ggcaactgcc aggttcggaa
    30241 aaacgttatc gtgactggaa aaaatggggc cacgggcgtc tgaatgtcac aagatcgctg
    30301 gaagaatctg cggatacctt cttctatcag gtggcctacg atatggggat cgatcgcctc
    30361 tccgaatgga tgggtaaatt cggttatggt cattacaccg gtatcgacct ggcggaagaa
    30421 cgttccggca acatgcctac ccgcgaatgg aaacagaaac gctttaaaaa accgtggtat
    30481 cagggtgaca ccattccggt tggtatcggt cagggttact ggacagcgac cccaatccag
    30541 atgagtaagg cactgatgat cctgattaat gacggtatcg tgaaggttcc tcatttgctg
    30601 atgagcaccg ccgaagacgg caaacaggtg ccatgggtac agccgcatga accgcccgtc
    30661 ggcgatattc attccggtta ctgggagctg gcgaaagacg gtatgtacgg tgttgctaac
    30721 cgccctaacg gtacggcgca taaatacttt gccagcgcac cgtacaaaat tgcggcgaaa
    30781 tccggtaccg ctcaggtctt cggtctgaaa gcgaacgaaa cctataatgc gcacaaaatt
    30841 gccgagcgtt tacgtgacca caaactgatg accgcctttg cgccatacaa caatccgcaa
    30901 gtggctgtcg ccatgattct ggagaacggt ggtgcgggtc cggcggttgg tacactgatg
    30961 cgccagatcc tcgaccacat tatgctgggt gataacaaca ccgatctgcc tgcggaaaat
    31021 ccagcggttg ccgcagcgga ggaccattaa tcatgacgga taatccgaat aaaaaaacat
    31081 tctgggataa agtccatctc gatcccacaa tgctgctgat cttactggca ttgctggttt
    31141 acagcgccct ggttatctgg agcgccagcg gtcaggatat tggcatgatg gagcgtaaaa
    31201 tcggccaaat cgcgatgggt ctagtcatca tggtggtgat ggcgcaaatt cctccacgcg
    31261 tttatgaagg ctgggccccc tatctctata tcatctgtat tattttgctg gtggcggtag
    31321 atgctttcgg tgccatctct aaaggtgctc aacgctggct ggacctcggt attgttcgtt
    31381 ttcagccgtc ggaaattgcc aaaatagccg taccactgat ggttgcgcgc tttatcaacc
    31441 gcgacgtttg cccgccatcg ttgaagaaca ctggcatcgc gctggtgctg atatttatgc
    31501 ccacgctgct ggtggctgca cagcctgacc tgggaacatc aatcctcgtt gcgctttccg
    31561 gtctgtttgt actgttcctc tctggcctta gctggcgtct gattggcgtc gcagtagtgc
    31621 tggtggcggc gttcattccg attctgtggt tcttcctgat gcatgattac cagcgccagc
    31681 gcgtaatgat gctcctagac ccggaatcag acccactcgg cgcgggctat cacattattc
    31741 agtctaaaat tgctattggc tccggcggat tacgcggcaa aggctggctg cacggcactc
    31801 agtcacagct tgaatttctc cccgaacgcc ataccgactt tatcttcgcg gtactggcgg
    31861 aagagctggg attagtgggc attctgattc tgctcgctct ctacattctg ctgatcatgc
    31921 gcgggctgtg gatagccgcc agagcgcaaa ccacctttgg tcgcgtcatg gctggtggct
    31981 taatgctgat attattcgtt tatgtcttcg taaatattgg tatggtaagc ggtattctgc
    32041 cggttgtagg ggttccgctc ccactggtca gttatggagg atcggcgcta attgtgctga
    32101 tggctgggtt cgggattgta atgtcaatcc acacccacag gaaaatgttg tcgaaaagcg
    32161 tgtaagaggt gcgcaatgcg taagcagtgg ctcgggatct gcatcgcggc aggaatgctc
    32221 gcggcatgta caagcgatga tggtcagcaa cagacggtaa gtgtaccgca gcctgcggta
    32281 tgtaacggcc ccatagttga aattagcggg gcggacccgc gtttcgaacc actgaacgcg
    32341 acggcaaatc aggattacca gcgcgacggt aaaagctaca aaatcgtgca ggatccgtct
    32401 cgatttagcc aggcgggact ggcggcaatc tatgatgccg aacccggcag taacctgacg
    32461 gcctctggcg aagctttcga tccgacacag ctgacggcgg cccatccaac gcttccgatc
    32521 cccagctacg ccagaatcac taacctggct aacgggcgaa tgatcgtggt gcgcattaat
    32581 gatcgcggcc cttacggcaa cgaccgcgtt atttcacttt ctcgcgcggc agctgaccgt
    32641 cttaacacgt caaacaacac caaagttcgt atcgatccga ttattgtcgc ccaggatggt
    32701 tcgctttctg gtcctggtat ggcttgtacc acagtcgcca aacagactta cgccctgcca
    32761 gcacctcccg atttaagcgg tggcgcggga acaagttcag tgtctggccc gcagggtgac
    32821 attcttccgg tcagtaattc gacgctaaaa agcgaagatc cgaccggcgc gccggtaacc
    32881 agtagcggtt tcctcggcgc tccaacgacc ttagcgcctg gtgtactgga aggcagcgaa
    32941 ccgacgcctg cgccacagcc cgttgttaca gctccgtcga caacgcctgc aacctcgcct
    33001 gcaatggtga caccgcaagc cgcctcgcaa agcgccagcg gcaactttat ggtgcaagtc
    33061 ggggccgtaa gcgatcaggc tcgtgcgcaa cagtaccaac agcaactggg acagaagttc
    33121 ggcgtccccg gtcgcgtaac tcaaaatggc gcggtctggc ggatccagct tggcccattc
    33181 gccagcaaag ccgaagccag taccttgcag caacgtttgc aaaccgaagc ccaattacag
    33241 tcatttatta ctaccgcgca gtagcgtaaa gcaggcatct gatgtgtcaa tttctgtaaa
    33301 tgacgttaac aaagtcacgc ggaaagtcag atgcctgccg gtagtgcatt tgctatagta
    33361 gggcactttt ttaattccat cacggatgtc gtagttcaga ccatgaatac cattttttcc
    33421 gctcgtatca tgaagcgcct ggcgctcacc acggctcttt gcacagcctt tatctctgct
    33481 gcacatgccg atgacctgaa tatcaaaact atgatcccgg gtgtaccgca gatcgatgcg
    33541 gagtcctaca tcctgattga ctataactcc ggcaaagtgc tcgccgaaca gaacgcagat
    33601 gtccgccgcg atcctgccag cctgaccaaa atgatgacca gttacgttat cggccaggca
    33661 atgaaagccg gtaaatttaa agaaactgat ttagtcacta tcggcaacga cgcatgggcc
    33721 accggtaacc cggtgtttaa aggttcttcg ctgatgttcc tcaaaccggg catgcaggtt
    33781 ccggtttctc agctgatccg cggtattaac ctgcaatcgg gtaacgatgc ttgtgtcgcc
    33841 atggccgatt ttgccgctgg tagccaggac gcttttgttg gcttgatgaa cagctacgtt
    33901 aacgcactgg gcctgaaaaa tacccacttc cagacggtac atggtctgga tgctgatggt
    33961 cagtacagct ccgcgcgaga tatggcgctg atcggccagg cattgatccg tgacgtaccg
    34021 aatgaatact cgatctataa agaaaaagaa tttacgttta acggtattcg ccagctgaac
    34081 cgtaacggcc tgttatggga taacagcctg aatgtcgacg gcatcaaaac cggacacact
    34141 gacaaagcag gttacaacct tgttgcttct gcgactgaag gccagatgcg cttgatttct
    34201 gcggtgatgg gcggacgtac ttttaaaggc cgtgaagccg aaagtaaaaa actgctaacc
    34261 tggggcttcc gtttcttcga aaccgttaac ccactgaaag taggtaaaga gttcgcctct
    34321 gaaccggttt ggtttggtga ttctgatcgc gcttcgttag gggttgataa agacgtgtac
    34381 ctgaccattc cacgtggtcg catgaaagat ctgaaagcca gctatgtgct gaacagcagt
    34441 gaattgcatg cgccgctgca aaagaatcag gtcgtcggaa ctatcaactt ccagcttgat
    34501 ggcaaaacga tcgagcaacg cccgctggtt gtgttgcaag aaatcccgga aggtaacttc
    34561 ttcggcaaaa tcattgatta cattaaatta atgttccatc actggtttgg ttaaaaattg
    34621 aacacttgaa agtgtaattt ccgtccccat atactaagca tcagtaaaaa actcccgcct
    34681 tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa
    34741 cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga
    34801 gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac
    34861 ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca
    34921 tatcgagcag gttgaaacac tgtatgaaga actaggcaaa atcgatattg tccgcatggt
    34981 tctgtaactc gcttctccgt tacccggccc ctggtcgggt aactcccctc tcctcgctgt
    35041 gatatacttt tcccccactt ttactcattc tccacggaga tgcagttttg tatcaggata
    35101 aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccag gctatgcatg
    35161 aattcaccga tacccgcgat gatagtaccc ttgatgaaat ctggctggtc gagcactatc
    35221 cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc
    35281 cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccgggg caacaggtga
    35341 tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc
    35401 ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg
    35461 acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta cgtattcgac
    35521 gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac
    35581 gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg
    35641 aagcgacgac taataatatt gcaccacgtt tactggaaaa tattttagcg ctactaaaca
    35701 atcctgactt cgaatatatt accgcttaat tgcatacctc aatggcccaa tttacactgg
    35761 gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta tactttcttt
    35821 atcacttgca gaaatgcggt gctacgcctt gctccgtaac tcaccccatt gattaaccta
    35881 ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat aagattattg
    35941 taattttttt aaatattatt tttccatgaa taaaattgga gtaagtgcgt ggatagtaat
    36001 aatcaaattg aaccctgttt aagcagaaag tcgtcagaag gtaaaccaca aatatttaca
    36061 accttacgaa atattgatct taaccttctg actatttttg aagctgtata tgtacataaa
    36121 gggatcgtta atgcagcgaa agtgcttaat ctgaccccct cggcaatcag tcagtctatt
    36181 cagaaactgc gcgttatatt ccctgaccca ttgtttattc gcaaaggcca gggtgtcact
    36241 cctaccgcat ttgcgatgca tctacatgag tatatcagtc agggccttga gtccattctt
    36301 ggcgcgctgg atatcgaagg aagctatgat aagcaacgaa cgataactat tgcgaccact
    36361 ccctcggtcg gagccctggt ccttcctgtc atctatcggg cgattaaaac tcactatccg
    36421 cagcttttac tgcgcaaccc gcccatcagc gacgcggaaa accaactcag tcagtttcaa
    36481 accgatctca tcatcgataa catgttttgc accaatcgta cggtgcaaca tcatgttctg
    36541 ttcaccgaca atatggtgtt aatttgccgc gagggaaatc cactactctc tttagaagat
    36601 gacagagaga ctatcgacaa cgctgcgcat gtactcctgt taccggaagg gcaaaatttc
    36661 agcggtctgc ggcagagagt tcaagagatg tttccggacc ggcaaatcaa tttcaccagc
    36721 tacaacattt tgacaatcgc tgcactggtt gccaacagtg acatgttagc gattattccc
    36781 agccgttttt ataacctgtt tagtcgttgc tggccgctgg aaaaactgcc ttttccgtcc
    36841 ttaaatgagg agcaaataga tttctctatc cactacaaca aattcagcct gcgtgatccc
    36901 atcctgcacg gggtaataga tgttatccgt aatgcatttt gaaaaagtat tgttacagcc
    36961 agatcacagc gcagaacgat aaaatggcac aatccaacaa caattttaca ttttcgcgac
    37021 cgctttggct gctttcaggt ccgtttcaat gatatactgc cagtcgttaa ttcaaaaata
    37081 gttgataatt acaacaatct attgaattga aacgctttcc ttcgtaattc gcaactggaa
    37141 cacgcacgct atgagtaaac ccattgtgat ggaacgcggt gttaaatacc gcgatgccga
    37201 taagatggcc cttatcccgg ttaaaaacgt ggcaacagag cgcgaagccc tgctgcgcaa
    37261 gccggaatgg atgaaaatca agcttccagc ggactctaca cgtatccagg gcatcaaagc
    37321 cgcaatgcgc aaaaatggcc tgcattctgt ctgcgaggaa gcctcctgcc ctaacctggc
    37381 ggaatgcttc aaccacggca cagcaacgtt tatgatcctc ggcgctattt gtacccgccg
    37441 ttgtccgttc tgtgatgttg cccacggtcg cccggtagct cctgatgcca atgaaccagt
    37501 gaaactggcg cagaccattg ccgatatggc gctgcgttat gtggttatca cctccgttga
    37561 ccgtgatgac ctgcgcgatg gcggtgccca gcactttgcg gattgcatta ctgccattcg
    37621 ggaaaaaagc ccgcaaatca aaattgaaac tctggtgccg gatttccgtg gtcgtatgga
    37681 tcgtgctctg gatattctga ctgcaacgcc accagatgtg ttcaaccata acctggaaaa
    37741 cgtaccgcgt atttaccgtc aggtacggcc tggtgcagat tacaactggt cgctgaagct
    37801 gctggaacgc tttaaagaag cgcatccgga aatcccgacc aagtctggtc tgatggtggg
    37861 actgggtgaa accaatgaag aaattattga ggtaatgcgc gacctgcgcc gtcatggtgt
    37921 gacgatgtta acgctggggc aatatttgca gccaagccgc catcacctgc cggttcaacg
    37981 ttacgttagc ccggatgagt tcgacgaaat gaaagccgaa gcgctggcga tgggctttac
    38041 ccatgctgca tgcggtccgt ttgtccgctc ttcttaccac gccgatttgc aggcgaaagg
    38101 gatggaagtt aagtaatcct gtaataatta cttacatctg tctgataaaa aacccgcttt
    38161 ttg
//
LOCUS       DAZPEP010000024        37813 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZPEP010000024 DAZPEP010000000
VERSION     DAZPEP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.024"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUT9_001198"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="ACHUT9_001198"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFR1347722.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="ACHUT9_001199"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="ACHUT9_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFR1347723.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUT9_001200"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="ACHUT9_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFR1347724.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUT9_001201"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="ACHUT9_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFR1347725.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUT9_001202"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="ACHUT9_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFR1347726.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="ACHUT9_001203"
     CDS             5280..5738
                     /locus_tag="ACHUT9_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFR1347727.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="ACHUT9_001204"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="ACHUT9_001204"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFR1347728.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="ACHUT9_001205"
     tRNA            6790..6874
                     /locus_tag="ACHUT9_001205"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="ACHUT9_001206"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="ACHUT9_001206"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUT9_001207"
     CDS             complement(7237..7914)
                     /gene="narI"
                     /locus_tag="ACHUT9_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1347729.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUT9_001208"
     CDS             complement(7914..8624)
                     /gene="narJ"
                     /locus_tag="ACHUT9_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1347730.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUT9_001209"
     CDS             complement(8621..10159)
                     /gene="narH"
                     /locus_tag="ACHUT9_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1347731.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10156..13899)
                     /locus_tag="ACHUT9_001210"
     CDS             complement(10156..13899)
                     /locus_tag="ACHUT9_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFR1347732.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUT9_001211"
     CDS             complement(14292..15683)
                     /gene="narK"
                     /locus_tag="ACHUT9_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFR1347733.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16022..17818
                     /gene="narX"
                     /locus_tag="ACHUT9_001212"
     CDS             16022..17818
                     /gene="narX"
                     /locus_tag="ACHUT9_001212"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFR1347734.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17811..18461
                     /gene="narL"
                     /locus_tag="ACHUT9_001213"
     CDS             17811..18461
                     /gene="narL"
                     /locus_tag="ACHUT9_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFR1347735.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUT9_001214"
                     /gene_synonym="iatA"
     CDS             complement(18462..19856)
                     /gene="ychO"
                     /locus_tag="ACHUT9_001214"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFR1347736.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUT9_001215"
     CDS             20041..20394
                     /gene="ychN"
                     /locus_tag="ACHUT9_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFR1347737.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUT9_001216"
     CDS             complement(20438..21133)
                     /gene="chaC"
                     /locus_tag="ACHUT9_001216"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFR1347738.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUT9_001217"
     CDS             complement(21291..21521)
                     /gene="chaB"
                     /locus_tag="ACHUT9_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFR1347739.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUT9_001218"
     CDS             21791..22891
                     /gene="chaA"
                     /locus_tag="ACHUT9_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFR1347740.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23294..23401
                     /locus_tag="ACHUT9_001219"
     CDS             23294..23401
                     /locus_tag="ACHUT9_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1347741.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            23830..23937
                     /locus_tag="ACHUT9_001220"
     CDS             23830..23937
                     /locus_tag="ACHUT9_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1347742.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            24365..24472
                     /locus_tag="ACHUT9_001221"
     CDS             24365..24472
                     /locus_tag="ACHUT9_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1347743.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24621..25475)
                     /gene="kdsA"
                     /locus_tag="ACHUT9_001222"
     CDS             complement(24621..25475)
                     /gene="kdsA"
                     /locus_tag="ACHUT9_001222"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFR1347744.1"
                     /translation="MKQKVVSIGDINVANDLSFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(25511..26320)
                     /gene="sirB1"
                     /locus_tag="ACHUT9_001223"
     CDS             complement(25511..26320)
                     /gene="sirB1"
                     /locus_tag="ACHUT9_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFR1347745.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(26324..26716)
                     /gene="sirB2"
                     /locus_tag="ACHUT9_001224"
     CDS             complement(26324..26716)
                     /gene="sirB2"
                     /locus_tag="ACHUT9_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFR1347746.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26713..27546)
                     /gene="prmC"
                     /locus_tag="ACHUT9_001225"
     CDS             complement(26713..27546)
                     /gene="prmC"
                     /locus_tag="ACHUT9_001225"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFR1347747.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27546..28628)
                     /gene="prfA"
                     /locus_tag="ACHUT9_001226"
     CDS             complement(27546..28628)
                     /gene="prfA"
                     /locus_tag="ACHUT9_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFR1347748.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28670..29926)
                     /gene="hemA"
                     /locus_tag="ACHUT9_001227"
     CDS             complement(28670..29926)
                     /gene="hemA"
                     /locus_tag="ACHUT9_001227"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFR1347749.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            30140..30763
                     /gene="lolB"
                     /locus_tag="ACHUT9_001228"
     CDS             30140..30763
                     /gene="lolB"
                     /locus_tag="ACHUT9_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFR1347750.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30763..31614
                     /gene="ispE"
                     /locus_tag="ACHUT9_001229"
     CDS             30763..31614
                     /gene="ispE"
                     /locus_tag="ACHUT9_001229"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFR1347751.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31765..32712
                     /gene="prs"
                     /locus_tag="ACHUT9_001230"
     CDS             31765..32712
                     /gene="prs"
                     /locus_tag="ACHUT9_001230"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFR1347752.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            32836..34515
                     /gene="dauA"
                     /locus_tag="ACHUT9_001231"
     CDS             32836..34515
                     /gene="dauA"
                     /locus_tag="ACHUT9_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFR1347753.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKTNSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34570..34848)
                     /gene="ychH"
                     /locus_tag="ACHUT9_001232"
     CDS             complement(34570..34848)
                     /gene="ychH"
                     /locus_tag="ACHUT9_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFR1347754.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            35125..35709
                     /gene="pth"
                     /locus_tag="ACHUT9_001233"
     CDS             35125..35709
                     /gene="pth"
                     /locus_tag="ACHUT9_001233"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFR1347755.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            35826..36917
                     /gene="ychF"
                     /locus_tag="ACHUT9_001234"
     CDS             35826..36917
                     /gene="ychF"
                     /locus_tag="ACHUT9_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFR1347756.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9415 a         9791 c         9238 g         9369 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcgcttc cttaaataat caaaacctgt
     7081 tactccgagc taattttgcc cgatggcacc acgtttaccg ggcctacaat atcctcccct
     7141 gtaggtcgga taagcgaagc gcatccgaca actcaccccg caccgtgccc ataaaaaaac
     7201 cccgccgaag cagggtctat gtgaactaaa attcgcttag tgacgagcgc gcaccagctg
     7261 gtacttacgt gtcagatact ccaccggtac gctccagatg tgtaccagac gcgagaacgg
     7321 gaacagcagg aataacgtca tccccagcac caggtgcaga cggaagataa acgccacacc
     7381 atcgaggtgt tgagaagcgc caccgtggaa ggtcaccacc gactgcgccc agccaaccag
     7441 tttcatcatc tcgctaccgt ccatatgctg agcggagaac ggaatggtca acaggcccag
     7501 cgcgcactgg ataacgagca gcgacaggat caggatatcc gctccggtag tggttgcacg
     7561 cacgcgtggg ctgaacagac gacgcttcag caacagcacg ccgccaatca gacacagcac
     7621 gccgctggca ccaccagcaa acatcgccat tttctgtttc acttcaatcg gcagccaggc
     7681 ttcatacatc cagtgcggcg tcagcatacc gaagaagtga ccaacaaaaa tccccagaat
     7741 accgatatgg aacaggttcg acgccaggtt catccctttg cgatccagca tctggctgga
     7801 cgccgcgcgc caggtgtact gcccgtagtc ataacgcagc cagctaccaa tcaggaagac
     7861 cgccccggct atgtacgggt agatatcaaa gaagaacata ttcaggaatt gcattagtgc
     7921 tgtcctccgg tggtgatatt cagatattgc ggcgcgacgg caccggcaaa gcgacgctga
     7981 tgagcagtga ttgctgaatc accgcagcct ttgtcagcaa agaatttaac ctgctcttct
     8041 tcccagacag catccagcgc ctgcggcgta tcatcgcgcg cttcgtcggc aattttttcc
     8101 gccactttgt cgctgtcgat agcggtattg gccaatttca gcagcagatc aaacatcacg
     8161 gcataacggc tttcacgctg ttgcagacgc gcgctcagca atgccagaat cggcgcgata
     8221 tctttcaaac cttccacggc ttcgctttgc ggcagctgcg acaggtactc cagatacagc
     8281 ggcagatggt ccggtaattc gcggctgttt aactgcaagc cgtgctgctc gtactgcgcc
     8341 agcaggtcca ccatcgcctg gccgcggtca cgggattcac cgtgaacatg ttcaaatagc
     8401 aacagtgacg tggcgcggcc acggtcgaac agttcgctgt actgggcctg ggcatcgagc
     8461 ggatccatcg ccgttaaatc gcgcaggaaa atgcccagcg catgggcatc ttctttcgac
     8521 agatttttcg acgcagcaat cgcctcaaac atctcttgtt gatgctgcca taaggcagca
     8581 tccggatatt caaggagacg cgatacaatc acgagttcga tcatggatgc ggctccgttt
     8641 tgctggtcac atcgatggca tcaatacgac ggctgttgaa cagattgaat ttggtatctg
     8701 aaccgtggca gccatcacca aaggtaaagc cgcagccatt tttctccggg aaggcttccc
     8761 gtgccagttc acgatgacta ctcggcacca caaagcgatc ttcgtagtta gcaatcgcca
     8821 gataacggta catctcctgt gcctgggctt cggtcagacc gacctcttcc agcgcacggg
     8881 tatcaacttt accgtcaacg gtttcagcac gtttgtaatg acgcatcgcc agcatacgtt
     8941 tcagtgcgcg cagtaccggt ttggtatcac cggcggtcag cagattcgcc agatactgta
     9001 ccggaatacg caagctttcg acgtctggca gaatgccgtt gctacccagc tcacccgcgt
     9061 ctgctgcaga ctgaatcgga gacagaggcg gcacgtacca gaccatcggc agtgtgcgat
     9121 attctggatg cagcggcagc gccagtttcc attccattgc cattttataa accggcgact
     9181 gctgtgcggc ttcaataacg ctcagcggaa taccgtcttt aatcgcctgc tcgatgactt
     9241 tcggatcgtt cggatcgagg aacacctcca gctgacgctg gtaaagatct ttctcgttct
     9301 cggtgctggc tgcacgttca atcgcgtcgg catcgtacag cagcacgcca agataacgga
     9361 tacgaccgac acaggtttct gagcacacgg tcggctgacc cgcttcaata cgcggatagc
     9421 agaagatgca cttctcagac ttaccgctct tccagttgaa gtagattttt ttgtacgggc
     9481 atccggtgat gcacatacgc cagccacggc atttatcctg gtcgatcagg acgatgccat
     9541 cttcttcacg cttgtaaatc gcaccgctcg ggcaggtcgc cacacatgcc gggttcaggc
     9601 agtgttcgca caggcgcggc aaatacatca taaaggtgtt ttcgaactgg ctatacatcg
     9661 ccttctggat gttgtcgaag ttcttgtctt tcgccagttt gtcaaactca ccacccaggt
     9721 catcttccca gttcggccct ttttcgattt tcgccatccg ttccccggta atcagcgaac
     9781 gcggacgggc aatcggctgc gatttgctgc cttccggcgc ggtatgcagg ttctgatagt
     9841 caaaatcgaa cggctcgtaa taatcgtcga tccccggcag atgcgggtta gcgaagattt
     9901 tacccagcaa catggcacgg ttacccatgc gcggctgcag tttgccgttg attttacgga
     9961 tccagccgcc tttgtatttt tcctggtttt cccagtcagt cgggaagccc tgacccggct
    10021 tggtttccac gttgttgaac cacgcgtatt ccacgccttc acggctggtc cagacgtttt
    10081 tacaggtaac tgaacaggtg tggcagccga tgcacttatc gagattcagc accatgccga
    10141 cttgtgaacg aattttcatt ttacgctctc ctgtacctgg tcattgcctt cgccatctaa
    10201 ccagtcaatg ttcttcatct tacgcactac aacaaactca tcgcggttag aacctacggt
    10261 gccgtagtag ttaaagccgt atgccagatg ggcatagccg ccgatcatat gcgtcggttt
    10321 cggcgtgata cgggtgaccg agttatggat accaccacgc tgttgggtaa tttccgaacc
    10381 aggcaggtta acgatacgtt cctgcgcgtg gtacatcatg gtcatccctg ccggaacacg
    10441 ctggctgaca accgcacggg cagtcagagc accgttgctg ttgaatactt caatccagtc
    10501 gttatcggcg atacccagat ctttggcatc ggcttcactc aaccagacca ccggaccacc
    10561 gcgacctaaa gtcagcatca gcaggttgtc gctgtaggtg gagtggatac cccacttctg
    10621 gtgcggcgtc aggaagttga gcgctttttc cgggttgccg ttggatttct ggcctatcac
    10681 ttctttcacc gaacgggtgt cgatcggcgg acgataaacc agcaggcttt caccgaaatc
    10741 acgcatccac tggtgatcct gatacagttg ctgacgacca gagagcgtac gccatgggat
    10801 cagctcgtga acgttggtgt aaccggcgtt gtaagaaacg tgttcatctt ccagaccaga
    10861 ccaggtcggg ctggagataa ttttgcgcgg ctgtgcctga atatcgagga agcggatctt
    10921 ctcgtcttct ttattcagcg ccagatgcgt atggtcacga ccggtaaatt cgctcagggc
    10981 agcccaggct ttcacggcta cctgaccgtt ggtttccggt gccagtgtca ggatcatctc
    11041 tgccgcatca attgcggtgt tcagcatcgg ctggcctttc gccggacctt ccgctttggt
    11101 gtagttgagc ttacgcagca gatccatctc gctctgggtg ttccaggcaa tccctttacc
    11161 gccattaccg attttctcca tcaacgggcc aatagaggta aagcgttcgt aagtcgccgg
    11221 ataatcgcgc tctacgacca taatgtgtgg cgcggtttta cctgggatca ggtcgcactc
    11281 gccttttttc cagtctttca catccagcgg ctgcgccagt tcagcggcag agtcatgctg
    11341 gataggcagc gtgacgatgt cggtttcttt acccagatgg ccgacgcaca cttcggagaa
    11401 tttcttcgcg atggctttgt agatttccca gtcgcttttc gcttcccagg ccggatcgac
    11461 cgccgcagac agcgggtgaa taaacggatg catatccgaa gtattcatgt cgtctttttc
    11521 gtaccaagtc gccgtcggca aaatgatgtc ggaatagaga caggtgctcg acagacggaa
    11581 gtccagcgta accaccagat ccagcttgcc ttccagacca ttgtcctgcc agtccacttc
    11641 ttccggcttc acgccgccct gttgccccag atctttaccc tggataccgt gctccgtccc
    11701 cagcaggtac ttgagcataa actcatgacc tttaccggaa gaaccgagca ggttagaacg
    11761 ccagatgaac aggttgcgcg ggtggttttt accgttttct ggttgttctg ccgcaaaacg
    11821 gatggaaccc tctttcaggg atttcaccgt atagtccacc ggattcatcc cggctttttc
    11881 cgcttcgcca gcgatagtca gcgggttagt gcctaactgc ggtgcagacg gcagccagcc
    11941 catgcgttcc gcacggacgt taaagtcgat caagtgtccg gtatagcggg atttgtccgc
    12001 catcggtgac agcaactctt ccgccgtgac ggtttcataa cgccactggc tggagtggtt
    12061 atagaaataa gaagtgctgt tcatgtgacg cgccggacgc tgccagtcaa gggcaaacgc
    12121 cagcggctgc cagccggttt gcggacgcag tttttcctga cctacatagt gcgcccagcc
    12181 gcccccgctc tgaccgacac agccgcagaa aatcagcatg ttgatcagac cacgatagtt
    12241 catatcgagg tgataccagt ggttcagccc cgcaccgacg ataatcatcg aacgaccgtg
    12301 cgttttatca gcgttatcgg caaattcacg ggcgatgcga ataatctggc tgcgagaaac
    12361 gccggtaatc tgctcggccc aggccggggt ataagctttc acatcgtcat agctggttgc
    12421 acagttaacg tcgttcaggc cacgttccag accgtagttt gccagcgtca gatcataaac
    12481 ggtgctcacc agggcggtgc tgccatcagc cagttgcagg cgtttcaccg gcagtttgtg
    12541 cagcagcacg ttttccagtt ccactttgtt gaagtgttcc gtgccgtcgc caccaaagta
    12601 cgggaagcct acctcagcga tctcatcctg gctacccagc aggctcagtt gcagctcggt
    12661 ttcttcgcca gttttgccgt cgcgctgctc aagattccat ttgcccttct cgccccagcg
    12721 gaagccaata gaaccgttcg gcgcaaccat ttcgccattg gtattaaagg cgacagtttt
    12781 ccattccgga ttgttttcct ggcccagcgc atcaaccaga tcagcagcgc gcagcatacg
    12841 acctgcagcg tagtaaccgt cgcgttcttc cagcatcacc agcatcggca tgtcggtgta
    12901 gcgacgcaca tagtcggtga aatactggct tgggttgtcg aggtggaatt cacgcagcat
    12961 tacgtggccc atcgccagcg ccattgccgc atcggtgccc tgtttcggtg ccagccacag
    13021 atcgcacagt ttggcgattt cagcgtagtc tggtgtgacg gcaacagttt tggtgccttt
    13081 gtaacgcact tcagtaaaga agtgagcatc cggggtacgc gtctgcggca cgtttgaacc
    13141 ccaggcgatg atgtagctgg agttatacca gtcagcagat tccggtacgt cagtttgctc
    13201 gccccaggtt tgcggagacg caggaggcag gtcgcagtac cagtcgtaga agcttaagca
    13261 agtaccgcca atcagcgaga gatagcgtgc acccgatgcg taagaaacca tcgacattgc
    13321 cggaattggc gagaaaccag caacacggtc cgggccgtag tttttgatgg tgtaaacgtt
    13381 agatgcggcg atcagttcgt tcacctcctg ccaggaagaa cgaacaaatc caccgcgtcc
    13441 acgcgcctgc ttaaagcttt tcgctttatc ggcgtcttca atgatagaag cccatgcctc
    13501 aaccggatcg ctatgcagcg ccttcgcttc acgccacatt ttcatcaggc gtttgcgcat
    13561 catcgggtat ttcaggcggt tggcactgta aagataccag gagtagctgg caccgcgcgg
    13621 gcagccgcga ggttcatggt ttggcagatc cggacgggta cgcggatagt cagtctgctg
    13681 ggtttcccag gtgaccagac cgtttttgac gtagattttc cagctgcagg agccggtgca
    13741 gtttaccccg tgggtagagc ggacgatttt gtcatgctgc caacgctggc gatatccatc
    13801 ctcccagtca cggttggtat tgagaagctg gccatgccca tcggcaaagg tttcaccctt
    13861 ctgtttgaag tagcgaaacc ggtccaggaa tttactcatc ggttttctcc tgtgggagcc
    13921 tgtcggccct ctgataaatc gacattgcta atgtttaagg gcaaggtaac gctctgaaac
    13981 agcgtgggaa ttgataacga tcaagagtga tggggatgaa aaataaagta aattcctaag
    14041 ataccccttt aggataatta acagccacca cataacagac tgtaaattaa gacgttttat
    14101 acctgtagtt atttaaacta ttaagatttt tatgcagatg ggtattaagg agtattcccc
    14161 atgggtaaca tattaatggc tcttaatgaa atccggcgaa ataagccata taatatgagt
    14221 atgttgtaac taaatgacgc ataaaaaaag cgcgacatca tgccgcgcaa aggataatca
    14281 aatattaaga tttatttttt agaatgccga ccatataccg cccaggtaat cactacgcag
    14341 gcgatataga aaatcaaaaa taccttcatt gcgccgactg gcgaacccgt taatgccagc
    14401 gagctaccaa acgctttcgg gataaagaag ccaccaatag cgccaatcgc agagatgaaa
    14461 cccagcgccg ctgccgtgtc ggttgccgct tcacgcatcg cacgttcgtc agaaccccct
    14521 tctgctttca cgcgatccat tgtcagttta cggaagatca ctgaaatcat ctggaaagtg
    14581 gaaccactac ccagcccagc tgtcaggaac agcgccagga agaccgcgaa gaacgccatg
    14641 aagcttccgc cctgcccgtc agtcggtaag gtcaggaaca gcaggccgct gaaaatcgcc
    14701 atcagaataa agttcaccag cgtgacacga gttccgccca gacggtcaga taatgcacca
    14761 cctgcagaac gcgccagcgc accaataaac ggcccgaaga aagcgtattg cagaatctga
    14821 acatccggga actgcgtttt tgacagcatc gcaaagcccg cggagaagcc gataaaggag
    14881 ccgaaggttg ccagatacag caggctcata atccacagat gaccccgttt gagtaccggc
    14941 aactgctcct tgatggaggc tttcgaggta gcaagatcgt tcatgccaaa ccacgccgca
    15001 atggtgaaga tggcaaggaa cggcacccat atccaggacg cattcgccag atacagctca
    15061 gtcccatccg gctgtttgac gccctggcta ccaaatactg cgaaaatcga cagtgatacc
    15121 accagcggag caaccaactg catgacgctg acgcccatgt ttcccagacc accattcaga
    15181 cccagcgcgc caccctgctt ctgtttcgga aagaagaagc tgatgtttgc catactggat
    15241 gcgaagttcg cgccagcaaa gccgcatagc agagagatga tgatgaagac gctataaggc
    15301 gtggaggtat cctgcacggc aaaacccagc cagacgcaag gaataatcag aataccggtg
    15361 ctgaacgccg tccagcgacg accaccgaag ataggaacca taaaggagta tggaacacgt
    15421 aataacgcgc cagaaaccga aggcagcgca gtcaacataa atagctgatc ggtcgtaaaa
    15481 ttaaaaccga ctttcggtaa gttcaccgca acagcgctga acaacatcca tacgcaaaac
    15541 gccagcagca gacagggaac ggaaatccac aggttacggc tggcaatacg ttgaccgcgt
    15601 tgttgccaga acgcaggatc ttccggtcgc caatctgtaa tgacagctcc agtagccctt
    15661 tcgggggcgg atgagtgact catagacacc tctgatactc gtttcgatgt ctgccacctt
    15721 agtgtctgta gctaaaggca atttgatgta aatcaaacga taagataact ttatctatca
    15781 ttgatattta tcattaccca tagtgagtac agtgatttca taaaaattat gagatttttc
    15841 acggtgctgt aaaatcccta cccttaccga tgtaaagcga ctaaccacac ggcaaataag
    15901 gagtaactct ttccgggtat gggtatactt cagccaatag ccgagaatac tgccattcca
    15961 gaatgtatcg tcacattcat taaggttatt gctcatttaa agcctgaagg aagaggtttg
    16021 catgcttaaa cgttgtctct ctccgctcac cctggttaat caggttgcgc ttattgtgtt
    16081 gctttctact gctattggac tggcagggat ggcggtttct ggctggctgg tgcaaggcgt
    16141 tcagggcagc gcccatgcga tcaacaaagc gggatcgctg cgcatgcaaa gttaccgtct
    16201 gttggcggca gtgccattaa gtgagaaaga caagccctta attaaagaga tggaacaaac
    16261 ggcatttagc gccgagttaa ctcgagcagc agaacgagac gaacaactgg cgcaattaca
    16321 gggtttacaa gattactggc gtaatgaact gatccctgca ctgatgcgtg cacaaaaccg
    16381 cgaaacggtg tcagcggatg tcagccagtt tgttgccggg cttgatcagc tggtatctgg
    16441 ttttgaccgc accacggaaa tgcgcattga gacagtggta ctggtccatc gggtaatggc
    16501 ggtatttatg gcacttttac tggtgttcac tattatctgg ttgcgggcgc gactgctaca
    16561 accgtggcgg caactgctgg caatggcgag tgccgtcagt catcgcgatt ttacccaacg
    16621 cgcgaacatc agcgggcgca acgaaatggc gatgcttgga actgcattga acaatatgtc
    16681 tgcagaactg gccgaaagtt atgccgtact tgagcagcgg gttcaggaga aaaccgccgg
    16741 gctggagcat aaaaatcaga tcctctcttt tttatggcag gctaaccgcc gtttgcattc
    16801 ccgcgccccg ctgtgtgaac gcctgtcacc tgtactcaac ggcttacaga atttaaccct
    16861 gctacgtgat atcgaattgc gggtgtatga cactgatgat gaagagaatc atcaggagtt
    16921 tacctgccag ccagatatga cttgtgatga taaaggctgc cagctctgcc cgcgcggcgt
    16981 attacccgtt ggtgatcgcg gcacgaccct gaagtggcgg ctggctgact ctcatacgca
    17041 gtacggtatt ttgctggcga ccctgccgca ggggcgtcat cttagccatg atcaacaaca
    17101 actgctggat accctggttg aacaactcac cgccacgctg gcgctggatc gccatcagga
    17161 acgtcagcaa cagttgatcg tgatggaaga gcgtgccacc attgcgcgcg aactgcatga
    17221 ttctattgcc caatctctct cttgcatgaa gatgcaggtg agttgtttac agatgcaggg
    17281 cgatgcgctg ccagaaagca gccgcgaact gttaagtcag atccgtaacg aactgaatgc
    17341 atcctgggcg cagttgcgtg aattgctcac cacattccgc ttgcagctca ccgagcctgg
    17401 attacgtccg gcgctggagg cgagttgcga agagtacagc gccaaatttg gcttcccggt
    17461 gaagctggat tatcaattgc cgcctcgcct ggtgccttcg catcaggcaa tccacttgtt
    17521 gcaaattgcc cgtgaggcat taagtaacgc cctcaaacat tcgcaagcga gtgaagtcgt
    17581 ggtgacggtg gcgcaaaacg ataatcaggt caaactgacc gtccaggata acggctgcgg
    17641 cgtgcctgaa aatgccatcc gcagcaatca ctacggcatg ataataatgc gcgaccgtgc
    17701 gcaaagttta cgaggcgatt gccgcgtccg ccgtcgtgaa tcaggtggca ccgaagtggt
    17761 ggtcaccttt attcccgaaa aaactttcac agacgtccaa ggagataccc atgagtaatc
    17821 aggaaccggc tactatcctg ctgattgacg atcacccgat gctgcgaact ggcgtaaaac
    17881 agcttatcag tatggcacca gatatcaccg tggttggcga agcgagtaat ggcgaacagg
    17941 gtattgaact ggcggaatct cttgatcccg atctgatcct gttagatctc aatatgcccg
    18001 gcatgaacgg tctggaaacg ctggataaac tgcgcgaaaa gtccctctca gggcgcattg
    18061 tggtattcag cgtctctaac catgaagaag atgtggtcac cgcactgaaa cgcggcgcgg
    18121 atggttatct gttaaaagat atggaaccgg aagatctgct gaaagcattg catcaggcag
    18181 ctgctggcga aatggtatta agcgaagcat taacgcctgt tctggccgcc agcttgcgcg
    18241 ctaaccgtgc cactactgag cgcgatgtta accagttaac cccacgcgag cgcgatattc
    18301 tcaagctgat tgcccagggt ttgccgaaca agatgattgc ccgccgcctg gatatcaccg
    18361 aaagcacagt aaaagtgcac gtcaagcaca tgctgaagaa aatgaagctc aagtctcgcg
    18421 tggaagcagc ggtatgggtg catcaggagc gcattttctg attacggttc ccagcgcagt
    18481 tcgtcgtttg acaatgcatc gaacggttcg acaagcgtta gcgtgatctc attggaggag
    18541 acacgctgcc cctggttatc ttccaccacc accgacaatc gccagtgatt gctcgcccct
    18601 tccccgttct gccagtcagg catgatcagc gtccagccct ccgcgctgtt ggcttgtgcg
    18661 cccggcgtca aacttaatat ctgcgtatcg ccctgccaaa tcagttgccg aataccgtaa
    18721 cgactgcgga tttgtaattt cagcggcact gtttcgccag gttttagatc ccacggcggt
    18781 gtcgccagaa acaccgttaa cgtttttcgc tgtcggtact caagagtcgg tagattattt
    18841 cgctgcggat tatcatagcg actaccacgt aacgactgac tttcggcaac ctcgcccgca
    18901 gaaagttgtt ttttgagcgg cacaccaaag cggtaattaa gattcagtcc gaggttattt
    18961 tgattttcgc cactttcacc ctgtttatgc tgggccgtca cagtgactaa tggcacaggg
    19021 gtgtaattta atcccagact caacgcgacg ggattgtgat aacccgtacc agagttaaac
    19081 aaatcaacac gctcaccaaa atactgttct accctgacac tggtattgag gtgttgatag
    19141 aacggcatgc gcatccgggc tgtcaggtca tacccgcgcg ccatccgttg ttcctgcgtg
    19201 gctgtctgtt catgccatgc agcaaacggc tgataaaagt ttgccgatag tcgcaaatat
    19261 tcgccccacg cttcggcacc aaagcccgct cgctgaagat tttcgtccag caagttgtca
    19321 taaaaagtgt tataacccac cagccagttg ccgcgcgccc agcgttgccc aacgcccaca
    19381 ttgctcacca acccatcatc ctgctgagta agaccaagct ggctccaggt gagataacga
    19441 tcattatctt gtaacggcac aaaccagctt ccacgactgc cggtgaaatg tccttcgtta
    19501 tccactttga cgtcaacact ggcatttccc cacggtgata gccaggactc tacgtgctga
    19561 ttaacctgtt gactaagcgc gtcgcggact tttcccaatg cgaaagcttt tgcctgctcg
    19621 ccagtatcca gcccgttatc attcatactg gtttcgccga aatctttcac aatttcagca
    19681 aagtgttttt ccccatcatg gttttcggga gccatgccta aatccggcag accatcattg
    19741 ttattatcaa agggatttgc cgctttttgc tcgaaggtag attgtgcgtt agctgtaccg
    19801 cctgccgcca gcaagagtaa ataaaacggg ataatgcgag gaacgaaacg gctcaaaacg
    19861 tcggtaactt ccggtaataa tgaaaaacca tagccttaaa gaataacgat tactggcaac
    19921 ttttgtcgta ttttctcgcg ctttcaggaa aatccttaaa aaggtgaccg attggtcaga
    19981 cgctgcagaa gcagtaagat agaagatgag tgtctaattc cctgaataca ggagtaaata
    20041 atgcaaaaaa tcgtgatcgt tgccaatggc gcaccttacg ggagcgaatc cttgtttaac
    20101 agcttgcggc tggccattgc gttacgagag caggagagca atctggatct gcgtctgttc
    20161 ctgatgtctg atgcggtcac agccgggttg cgcgggcaaa aaccagggga aggctacaac
    20221 attcagcaaa tgctggagat ccttaccgct cagaatgtac cggtgaaatt gtgcaaaacc
    20281 tgtaccgacg ggcgcgggat tagcacactt cctcttattg atggggtgga aatcggtact
    20341 cttgtggaac tggcgcaatg gacgctgtca gccgataaag tgctcacatt ttaatgtttg
    20401 agggggcgtc tgacgcccca ctatgaggga agtttactca ggcgaatccc ggccgtaaca
    20461 caccatccgg ataattctcc gccagcagtt tttttaccga taccagcaaa tcattcagcc
    20521 catcgtcctg cattcccagt ttgataagct cctgttccag tgaaaacagg tattgtgcgt
    20581 tggttcccag cggaccgctc gccgccgcaa tcaacggcgc gatgacctga gcgcgagtgt
    20641 cagattcata ttctggatgt cgcgggtcca taataaacac aatggcgttt actgtgcgtc
    20701 catcatcaag atcaagctga caccaggttg gcagataaca gccggtaatc atctcgcgct
    20761 tccacaacag ggttagttcc tgctccagcg tctcttctgg tagtcgatag gcgacgcctg
    20821 tggtgcgtcc gccctctttc agtgcaagca tccgtcccgg ctggtgcgca gtcccgcgcc
    20881 cggcggtcag gcgcaggcaa aacgcgcgat gccatccaac cagtgtaccg gtgcataatt
    20941 cggtaaactc cagtgccgga ttccacatca acgatccata accgaagatc cacaccggtc
    21001 cctcattagg tcgacaagcc agcgtcgccg ccagcgaagc cgcccgttgt tctgctgacc
    21061 ataagagtga ttcttcaatg gcaccaaatg ccgttttaca atcggcattc atcaagaaat
    21121 cacgcgttat caccttacac ctccgccact gcatacttcc ctgcgacaac tttttggtgt
    21181 cttccggaca tttttaccgc tcattttgaa accactaacg ggacaatatg caatccacgg
    21241 accacttgca aggcaacacc gcacgaatag ctttaactaa gctaccggtt ttacgatttt
    21301 ttatgccatt tatcatcatc ccctttggca tattcatgct tcacagcagc ccaggccact
    21361 ttatgcgcgg tttcttcgcg actggcgtca tcacgccgat cttctttatc tttatattga
    21421 tcccatgcgc tgttgaacgc ttctttatag atatcctggg catgagacgg tagaacgtgc
    21481 tttacgcttt ccggcagatc gcttttcgtt ttatacggca tcgttgactc cttttacaaa
    21541 gttgaacatt aagtgtggtc aacaatatct actcttgcca aaacataaga attttcatgt
    21601 cagactgttc ttaagaaacg ctaaaacaaa cattgtctaa acgtaatact tatttttatg
    21661 ccccaatgag taaaaaatcg acagcggggt aaaaattcgt aaaatgttac cgatatccag
    21721 acactttctg ttatttttcc tacctatttt ttacattttc gataaattca tctgcaaagg
    21781 gataaccata atgtcaaatg ctcaagaggc ggtaaaaacc cgccacaagg agacttcgct
    21841 tattttcccg gttctggcgc tggtagtgct gttcctgtgg ggaagcagcc agacactacc
    21901 agtggtcatt gccatcaatc ttcttgcgct tattggtatt ttaagtagcg cctttagtgt
    21961 tgtccgtcat gcggacgtat tagcccatcg cctgggagaa ccttacggtt cgcttattct
    22021 tagcctttca gtggttattc ttgaagtcag tttgatttca gctttaatgg caaccggcga
    22081 cgccgcgcca acgctaatgc gtgatacgct ctattcaatc attatgattg ttaccggtgg
    22141 gctggttggc ttttcattat tgttgggcgg tcgtaagttt gccacccaat atatgaatct
    22201 gtttggtatc aagcagtatt taattgcgct gttccccctg gcgataatcg tactggtatt
    22261 tccaatggct ctgcctgcgg cgaatttttc aaccggtcag gcgttactgg tagcattaat
    22321 ttctgcggca atgtatggcg tatttttgct gatccagacc aaaacgcatc aaagtttgtt
    22381 tgtctacgag cacgaagatg acagtgatga cgacgacccg caccacggta aaccgtctgc
    22441 ccatagcagc gtgtggcatg ctatctggtt gattatccat ctgattgccg ttattgcagt
    22501 caccaaaatg aatgccagcc cgctggagac attgctcgac agcatgaatg cccctgtcgc
    22561 ctttactggc ttcctggtgg ctctgttgat tctgtcgccg gaaggtttag gtgcgttaaa
    22621 agcagtgttg aacaaccagg tccagcgcgc gatgaatctg ttctttggtt cagtgttagc
    22681 aaccatttcg cttaccgtac ctgtcgtcac gctaattgcc tttatgacgg gtaacgaatt
    22741 acagtttgca cttggtgcgc cagaaatggt ggtgatggtg gcctctttag tgctgtgcca
    22801 tatctccttc tccaccggac gtactaacgt gctcaatggc gcagcgcatc tggcactgtt
    22861 tgccgcctat ttgatgacga tatttgcctg acagttatat ctcctccgat gacggaaatc
    22921 gggggagata agataaattc ctgcttattg tatttcttca tactacctgg acagctcgtt
    22981 aatcagaatt gcgagcctgt accacaacct gcacgaaacc actgcaacag ctgttgaaaa
    23041 gccggaatgt actctttatt gcaaacattt cagggatgaa gtgaagatga attgagacgt
    23101 agtatccaca ccaagccgga attatccggt gaggcgcaat gttgcggggg ctttatccct
    23161 ggtggcattg gttgctggaa agagaaaacc cccgcacgtt gagaggtaaa acctgacaac
    23221 aaaacggggg ctaatcttga aaccagacac ttcaagaata gccgccttgt tgtcattaca
    23281 acaaggcggc tatatgacgc tcgcgcagtt tgccatgact ttctggcacg acctggcagc
    23341 accgatcctg gcggggatta ttaccgcagc gattgtcagc tggtggcgta accggaagta
    23401 aggtgaaaac gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg
    23461 gtaacagcct gcggccattt ttgtctttat ctcttccaca agtaaatatt ggcaacccga
    23521 gctggaagca tcatccagag cactcatgaa acgactgtaa cagctgttga aaaaccggaa
    23581 tgcactcttc attgcaaaca tttcagggat gaagtgaaga tgaattgagt cgtagtatcc
    23641 acaccaagcc ggcgttatcc ggtgaggcgc aatgttgcgg gggcttaatc cctagtggca
    23701 ttggttgctg gaaagagaaa acccccgcac gttgagaggt aaaacctgac aacagaacgg
    23761 gggctaatct tgaaccatca acaattcaag aatagccgca aaatgttgtc attacaacaa
    23821 ggcggctata tgacgctcgc gaagtttgcc atgattttct ggcacgacct ggcagcaccg
    23881 atcctggcgg ggattattac cgcagcgatt gtcggctggt ggcgtaaccg gaagtaaggt
    23941 gaaaacgggc gtcaggctgc cgtgatggca atatgcgccc gaaaccggcc gcaggggtaa
    24001 cagcctgcgg ccatttttgt ctttatctcc tccacaagta aatattggca acccgagctg
    24061 gaagcatcat ccagagcact catgaaacga ctgtaacagc tgttgaaaaa ccggaatgca
    24121 ctcttcattg caaacatttc agggatgaag tgaagatgaa ttgagacgta gtatccacat
    24181 caagccggcg ttatccggtg aggcgcaatg ttgcgggggc tttatccctg gtggcattgg
    24241 ttgctggaaa gagaaaaccc ccgcacgttg agaggtttaa cctgacaaca gaacgggggc
    24301 taatcttgaa ccagaacaat tcaagaatag ccgcaaaatg ttgtcattac aacaaggcgg
    24361 ctatatgacg ctcgcgcagt ttgccatgat tttctggcac gacctggcag caccgatcct
    24421 ggcgggaatt attaccgcag cgattgtcgg ctggtggcgt aaccggaagt aaggtgaaaa
    24481 cgggcgtcag gctgccgtga tggcaatatg cgcccgaaac cggccgcagg ggtaacagcc
    24541 tgcggccttt tttgtatctg gcacccatga aaaaagtctt aacgcagaac gctaagactt
    24601 tatttttcaa gcaaaaaaga ttacttgctg gtatccagtt cttcgaaacc tttcaccaga
    24661 tcatcaatcg ctttcatctg cttgaggaac ggttccagtt tagccagcgg cagcgcggat
    24721 ggaccatcac atttcgcatg ttccggatcc ggatgcgctt caataaacag ccccgccaga
    24781 cctaccgcca taccggctcg tgccagctca gccacctgag cacgacgacc accggaagct
    24841 gcgccaaacg gatcgcggca ttgcagtgcg tgggtcacgt cgaaaatcac cggcgagtta
    24901 ccagacactt tcttcataat gctgaagccc agcatatcga caaccaggtt gtcatagccg
    24961 aagttagcac cgcgatcgca aagaatcact ttttcgttgc cgccttcttt gaatttatca
    25021 acgatattac ccatctgtcc cgggctgaca aactgtggtt tcttgacgtt aattaccgca
    25081 ccggttttcg ccatggcttc aaccaggtca gtctggcgag caagaaacgc cggcaactga
    25141 atcacatcca cgacatcagc aacgggctgt gcctgacttg gttcgtgaac gtcggtgata
    25201 attttcacgc caaaagtctg cttcaactcc tggaagattt tcatcccttc ttccaggccc
    25261 ggtccacgat aagagtggat ggaggagcgg ttggctttgt caaaagaggc tttgaacacg
    25321 taagggatac ccagtttctg ggtcacggtt acgtagtgct cgcaaatgcg catcgccaga
    25381 tcgcgagatt ccagcacgtt cataccacca aacagtacga acgacaggtc atttgctacg
    25441 ttgatgtcgc caatgctaac cactttttgt ttcataggat cgccttaatc ttgagtaaaa
    25501 tgtcgattaa ttaatgcagc acaatatgtt tatgcgcgat gttatttatt tgcgcacgga
    25561 tcatttcgct gatcgggtct tccggacact gttcaacgaa ataacttaaa tcgttcaacg
    25621 caacgtgttc gcaatccagt tgcgcataaa tcaacccgcg atcgcgaatt tcatagggat
    25681 cttccgggtt gaattgtaat aaagcttcgc tggtgcgtaa cgccagctcc atctgatttt
    25741 cttccatcaa cgaggctttg agtgtatcca acaatttgcg gattacctca atgttatcag
    25801 cttcatcaag gtcttcataa aacagttccg ccgacgggct gatatttccc tttaaccata
    25861 cgtccagcat atgttcgctt aacgattcac cgttaaaagg attaatcagc caaatttcgc
    25921 catccggaca ttcaatgcgc aatatcagct gcgtagggaa aatcaccggc agcagcggca
    25981 aatcgagacg attcgcgacc cataataaaa ccgcacctaa tgataccgca ctgccctgtc
    26041 gattctttaa cacctggtcc agccataatg catcggaaag acgataaacg ccgcgtgagg
    26101 ctttaaatcc ccagtcgccg taaaacagcg cgatcagttt ttccagctgc tcttctaaag
    26161 gcagaagctg gctgatttct tccttcgcca gactaacgag acgctccagt tcgtcgtaaa
    26221 catcttgcga gggaaaatcg cggcggattg cttcgcaagc caggatcatg ccttcgcaca
    26281 atggcgcttt attaaattcg aaatcagcta acgatctcat gacttacccc agtaacggta
    26341 cttttgtggt ggcgagttta atgatgatgt acagcaccac caacgccagc gggaaggcaa
    26401 taatgcgcgc ctgctgactg tgcatacgac gataatcgag tgcaataaaa cccaaaacga
    26461 tataaatgat aactccaaac agcttttcag tcagccactg tgcctgcccg gaaaatggca
    26521 ggatgtgcgc tttagccatc aacgcaatgc cgcttaacaa taacaccgta tcgacaaccg
    26581 gcggcacaat gcgcgtccag cgagcaaatg cctgtggatg cttctgataa cgtagccaga
    26641 aacgtaaggt taatagccca acagaaagcg cgatactaat aagatgaaca ctaagcagtg
    26701 tagaaaaact tgtcattgat aatagcggcc gagcgttacg cgctcgttat caccatagtc
    26761 acggcaggtt tcgacgtcat gataccccgc gaggataaat gcttgtcgca ccgcttcgcc
    26821 ctgctgccag ccatgttcca gaagcagaaa gccgccggat accagcgcgt tacgcgactg
    26881 ttcgatgata tgcacgatgt ctgccattcc actgtctgcc gcaaccagcg cagtgagcgg
    26941 ctcaaagcgg acatcgcctt gttgaagatg tgggtcctgc tcgtcaatat acggcggatt
    27001 gctgacaatc atcgcaaact gctgcccggc tagcgcgcta aaccagtcgc tttgcagaat
    27061 gtggatattt ttgatcgcca gatgctgggc attacgttgt gccagggaga cagcatcagg
    27121 catacgatct acagcgataa tttcgcagtc cgggcgctcg ctagccagcg caagcgcaat
    27181 cgccccggta cccgtcccga gatcgagaat acggcaaggt tgttcaggca accgcgccag
    27241 tgcctgctcc accagacact ccgtatccgg gcgcggaatt aaggtcgctg gcgaaacaaa
    27301 taacggcaac gaccagaatt ctcgcacccc ggttaaatga gcaatgggtt caccatcgcg
    27361 acgacgtgtc agtagcgcat caagttgctg acattgttcg tcagtcagct gcgtttcacc
    27421 aaaggccaga ataaaagtcc gccccctgcc agtgacatac tccaataaga tttcagcgtc
    27481 acggcgtggg ctttcgctcg cctgaagttg gcttattgct tcacgtaacc agtattgata
    27541 ttccattatt cctgctcgga caacgccgcc agttggtcgg cctgatgttc ctggataatc
    27601 ggttcaatca gcatatccag cttaccttcc atcacttcat ccagacggta gagcgtcagg
    27661 ttgatgcggt gatcggtaac gcgcccctgc gggaagttgt aagtacggtt acggtcgctg
    27721 cgatcgccac tccccagcag gttacgacgg gtagacgctt cggcctgttg ccgttttgcc
    27781 atttctgcag cgtggatacg agcaccaagt acagaaagtg ctttagcttt gtttttatgt
    27841 tgtgaacgtt cgtcctgaca ttcaacaaca atcccggtcg gcaagtgagt aatacgaatt
    27901 gccgaatcgg tggtgttaac gtgctgacca cccgcccctg acgagcggaa agtatcaatg
    27961 cgtaaatctg ctgggttgat gtccggcagt tctgcgtcag gcagttctgg cattaccgca
    28021 acggtacaag cagaagtatg aatacgaccc tgcgattccg tagcaggaac acgctgcacg
    28081 cgatgaccgc cggattcaaa tttcagacga ccatacacac catcaccgct aattttggcg
    28141 atgatctctt tataaccacc atgttcaccc tcgctggcgc tcatgatttc tacccgccag
    28201 cggcgggctt cggcataacg gctgtacata cggaacagat cgcccgcgaa cagcgccgct
    28261 tcgtcgccgc cggttccggc tcggacttcg aggaaggcgt tacgttcgtc atcaggatct
    28321 tttggcagta acagaacctg taattgctgt tccagttgct cgcttttttc tttagcttcg
    28381 cgcagttcat cctgcgccat ctcacgcatt tcaggatcat cgagcatcat ctgtgcggtt
    28441 tcgatatctt cctgaacctg ttgccagtcg gtaaaacagc gcgaaacatc acttaactgc
    28501 gcatattcgc gtgataatgc gcgaaaacgt tcctggtcgg cgatagtttg cgcgtcaccc
    28561 agcaacgcct gaacttcttc atggcgttca tgcagggctt ccagtttggc aacgatagaa
    28621 ggcttcatag gcgtaaatgc accctgtaaa aaaagaaaat gatgtactgc tactccagcc
    28681 cgaggctgtc gcgcagaata ttcaggcgtt cgttatcccc gtcacgggcg gcctgttgaa
    28741 gtgatttcgt tggcgcatgg atcaagcggt tagtcagttt ccatgccaga tcctgcataa
    28801 tggcttgcgc gtcgccgccc tgctcaaggg ccgctaacgc tttggcggtt aactcatcgc
    28861 gaacgtgctc tgcctggctg cgatactcgc gaatggtttc gctggcgctt tgtgctcgca
    28921 gccacgccat aaattcgctg gtttcctgag cgacaatagt ttccgcctca accgctgcgg
    28981 ctttacgctg cgccaggttg tgcgaaatga tgctttgcag atcgtccaca ctataaagat
    29041 aagcattcgc cagtttgcca acttccggct caacatcgcg cggaacggca atatccacca
    29101 acagcattgg ttggttacga cgacttttta atgcgcgctc caccatgcct ttcccgataa
    29161 tcggtaacgg gctggcggtg gaactgatga tgatatcggc ttcgcgcaga cgttcgtcga
    29221 tctcactcag ggcaatcact tccgcgccaa cttcatctgc cagaatttgg gcacgttcgc
    29281 gagtgcggtt ggcgataatc atcttctgta ctttatgttc gcgcagatga cgcgctacca
    29341 gctcgatggt ttcgcccgcg cctaccagca acactgtgac cgtagagagc gattcaaaga
    29401 tctgccgcgc cagcgtacaa gccgcaaaag cgacagacac agcactggca ccgatatctg
    29461 tttcagtgcg aacgcgtttc gctacagaga aagatttctg gaacatgcgt tccagttcgc
    29521 tggccttcat atgacctttt tgcgaatcgg caaacgcttt tttaacctga ccgaggatct
    29581 gcggctcccc aagaaccagt gaatccaggc cgctggcaac acgcattaaa tggctaaccg
    29641 cgtcgttatc ctgatgccag tagaggcttt tacgcagatc ttcttcatta agattgtgat
    29701 aatcgcaaag ccagcggatt aacgcctctt gcaggttatc ctgctcttca acgctaagat
    29761 aaagttccgt gcggttgcac gtcgacagca ccacgccgcc ctgcaccatc ggctgcgcaa
    29821 gcaggctgtc aagcgcctga tcgagcttat ccggcgaaaa cgatacacgt tctcgcagcg
    29881 atacaggtgc cgttttatgg ttgataccga gtgctaaaag ggtcatgtct gcgggaaata
    29941 ataccaacgt tgatatggtt agtctgcttg catcatacag gatgcgtaag atcaataaaa
    30001 gagagcgccc ccttttggag taattgccga aagccgttag attctggcaa ttaagacaac
    30061 ttgaacatag acgatagcgg acggtaacgc tagcattaag ggttataact gcaacgtatc
    30121 tcaaggactt gtcatcacta tgcccctgcc cgattttcgt cttatccgcc tgctaccgct
    30181 ggctgctctt gtgctcactg cctgttccgt taccacgccc aaaggtcctg gcaaaagccc
    30241 ggattcgcca caatggcgtc agcatcagca agacgtgcgc aatcttaatc agtatcagac
    30301 tcgcggcgcg ttcgcttata tttctgacca acaaaaagtg tacgcccgct ttttctggca
    30361 gcaaaccggc caggatcgct accgtctgct gctcactaac ccattgggca gcacggaact
    30421 ggagctgaat gctcaaccgg gtaacgtgca gttagtcgac aataaaggtc agcgttatac
    30481 cgccgatgac gccgaagaga tgattggcaa attgaccgga atgccaattc cgctcaacag
    30541 cttgcgccaa tggattttag gtttaccggg tgatgcaacc gactacaaac tggacgacca
    30601 gtaccgcctg agcgaaatta cctacagcca gaatggcaaa aactggaagg ttgtttatgg
    30661 tggttatgac accaaaacgc aacctgcgat gccagccaat atggaactca ccgacggtgg
    30721 tcaacgcatc aagttaaaaa tggataactg gatagtgaaa taatgcggac acagtggccc
    30781 tctccggcaa aacttaatct gtttttatac attaccggtc agcgtgcgga tggttaccac
    30841 acgctgcaaa cgctgtttca gtttcttgat tacggcgaca ccatcagcat tgagcttcgt
    30901 gacgatgggg atattcgtct gttaacgccc gttgaaggcg tggaacatga agataacctg
    30961 atcgttcgcg cagcgcggtt gttgatgaaa actgcggcag acagcgggcg tcttccgacg
    31021 ggaagcggtg cgaatatcag cattgacaag cgtttgccga tgggcggcgg tctcggcggt
    31081 ggttcatcca atgccgcgac ggtcctggtg gcattaaatc atctctggca atgcgggcta
    31141 agcatggatg agctggcgga aatggggctg acgctgggcg cagatgttcc tgtctttgtt
    31201 cgggggcatg ccgcgtttgc cgaaggcgtt ggtgaaatac taacgccggt ggatccgcca
    31261 gagaagtggt atctggtggc gcaccctggt gtaagtattc cgacaccagt gatttttaaa
    31321 gatcctgaac tcccgcgcaa tacgccaaaa aggtcaatag aaacgttgct aaaatgtgaa
    31381 ttcagcaatg attgcgaggt tatcgcaaga aaacgttttc gcgaggttga tgcggtgctt
    31441 tcctggctgt tagaatacgc cccgtcgcgc ctgactggga caggggcctg tgtctttgct
    31501 gaatttgata cagagtctga agcccgccag gtgctagagc aagccccgga atggctcaat
    31561 ggctttgtgg cgaaaggtgt taatctttcc ccattgcaca gagccatgct ttaagccggg
    31621 caagctgagt ttcggtgaca acgtcacctt gttccagacg ttgcatcgcg ctctttaata
    31681 caccgcctgg aaaggatcat gcctggcccg cacagttttc ggcagattct ttccaccaat
    31741 ggacgcatgc ctgaggttct tctcgtgcct gatatgaagc tttttgctgg taacgccacc
    31801 ccggaactag cacaacgtat tgccaaccgc ctgtacactt cactcggcga cgccgctgta
    31861 ggtcgcttta gcgacggcga agtcagtgta caaatcaacg aaaatgtacg cggtggtgat
    31921 attttcatca tccagtccac ttgtgcccct actaacgaca acctgatgga attagtcgtt
    31981 atggttgatg ccctgcgtcg tgcttccgca ggtcgtatca ccgctgttat cccctacttt
    32041 ggctatgcgc gccaggaccg tcgcgtccgt tccgctcgtg taccaatcac tgcgaaagtg
    32101 gttgcagact tcctctccag cgtcggtgtt gaccgtgtgc tgacagtgga tctgcacgct
    32161 gaacagattc agggtttctt cgacgttccg gttgataacg tatttggtag cccgatcctg
    32221 ctggaagaca tgctgcagct gaatctggat aacccaattg tggtttctcc ggacatcggc
    32281 ggcgttgtgc gtgcccgcgc tatcgctaag ctgctgaacg ataccgatat ggcaatcatc
    32341 gacaaacgtc gtccgcgtgc gaacgtttcc caggtgatgc atatcatcgg tgacgttgca
    32401 ggtcgtgact gcgtactggt cgatgatatg atcgacactg gcggtacgct gtgtaaagct
    32461 gctgaagctc tgaaagaacg tggtgctaaa cgtgtatttg cgtacgcgac tcacccgatc
    32521 ttctctggca acgcggcgaa caacctgcgt aactctgtaa ttgatgaagt cgttgtctgc
    32581 gataccattc cgctgagcga tgaaatcaaa tcactgccga acgtgcgtac tctgaccctg
    32641 tcaggtatgc tggccgaagc gattcgtcgt atcagcaacg aagaatcgat ctctgccatg
    32701 ttcgaacact aatcgaaccc gctcaaagac ccgctgcggc gggttttttt gtctgtaata
    32761 tccatttgta tgacctatgc ctccttcacc tgccatttag ttgacagatg atgcgctcac
    32821 ggatgaaaca ttattgtgaa caaaatattt tcctcacatg tgatgccttt ccgcgctctg
    32881 atcgacgctt gctggaaaga aaaatatact gccgcacggt ttacccgtga cctgattgcc
    32941 gggataaccg tcgggattat tgctatcccg ctggcgatgg cgttggctat tggtagtggt
    33001 gtggcacccc agtacggttt atataccgca gctgttgcgg ggattgtcat tgctctgacg
    33061 ggtgggtcac gctttagcgt ttccggtccg actgcggcat ttgtggtaat tctctatccc
    33121 gtgtcgcaac agtttggact ggcaggactg ctggttgcga ccttgctgtc ggggatcttt
    33181 ttgattctga tgggtctggc acgctttggt cgcctgattg agtatattcc ggtttccgtc
    33241 accttaggtt tcacctcggg tatcgggatc accatcggta ccatgcagat taaagatttt
    33301 ctcggtctgc aaatggccca tgtcccggaa cattatctac aaaaagtcgg cgcattattt
    33361 atggcgctgc cgaccattaa tgtgggtgat gctgccattg gcattgtgac gctaggtatt
    33421 cttgtttttt ggccgcgtct gggcattcgt ttacccggtc accttccggc cttgctggct
    33481 ggttgcgcgg tgatggggat tgttaacctg ctcggcggac atgttgctac catcggttcg
    33541 caattccact acgtcctggc cgatggttct cagggtaacg gtattccgca actgctgccg
    33601 caactggtgc tgccgtggga tctgcctaat tcagaattca cgctaacctg ggattctatt
    33661 cgcacactgc tgcctgcggc attctcaatg gcaatgctcg gcgcaatcga atctctgctc
    33721 tgcgccgtgg tactggatgg tatgaccggg acgaaacaca agacgaacag cgaactggtt
    33781 ggacagggac tggggaatat tatcgctccg ttctttggtg gtattaccgc tacagctgcc
    33841 atcgcgcgtt ctgccgctaa cgtccgtgcc ggggcaactt cccctatctc ggcggtgatc
    33901 cactctattc tggttattct tgccctgctg gtactggcac cgctgctctc ctggctgccg
    33961 ctttccgcta tggcagccct gctgttgatg gtggcgtgga acatgagtga agcgcataaa
    34021 gtggtcgact tgctgcgtca tgcaccgaaa gatgacatca ttgtcatgct gctgtgcatg
    34081 tcgctgaccg tgctgtttga tatggttatt gccatcagcg tggggatcgt gctggcatcg
    34141 ctgctgttta tgcgtcgtat cgcacgtatg actcgcctgg caccggtagt cgtagatgtt
    34201 ccagacgatg ttctggtact gcgcgttatt ggcccgctgt tttttgctgc tgctgaaggc
    34261 ttgttcacgg acctggagtc acgtcttgaa ggcaaacgga ttgtgattct gaagtgggat
    34321 gccgttccgg tacttgatgc tggtggtctt gatgcgttcc agcgttttgt gaagcgtctg
    34381 cccgaaggat gtgaactgcg cgtgtgcaac gtggaattcc agccactgcg cactatggct
    34441 cgcgcaggca ttcaaccgat cccgggacgc ctcgcgttct tcccgaatcg tcgcgcggcg
    34501 atggcggatt tataagtacg agattgacca gtcagcgcaa actgactggt cagcaaactg
    34561 cattatttgt tagctatgac ggcgattatc gctacggcgg caacgtttgt catagtgacg
    34621 aacccaccag taacggtcgc acacctgttc atgcccgcca acacgggcac ccgccagcca
    34681 gagaatggca ccgacgaaaa tacttagcac tgcaccatgt gcgaaatact ggggcatatt
    34741 aaactgtggt aactggttga ggattgaata ccccacgccg accaccatta ccaccagccc
    34801 caaccccatg agcacgttac cgagtaacga agcgtttttg cgtttcatat gtcacctccg
    34861 gaactttctg ggttgtaaca gggaataccc ctcttcctta tgtgtaaagt atagacaaca
    34921 ccctgtgggt tatgtgcggg cgtgatcaca attacaaccc ttatttcaac aaaactttac
    34981 aaataaacgc ctgaacacct tgactttcta atagtccact caggcaatta ttacgttatc
    35041 tgcatttgat acgcagtttt ttgtcaagcg gggccgcaac cagtaaacta cgcgccagtt
    35101 atgtacacac tcaggacaaa aaacgtgacg attaaattga ttgtcggcct ggcgaacccc
    35161 ggtgctgaat acgccgcaac gcgacataat gctggtgcct ggttcgttga cttactggca
    35221 gagcgtttgc gcgctccgct gcgcgaagag gctaaattct ttggttatac ttcgcgagtc
    35281 actcttggag gcgaagatgt ccgcctgtta gtcccgacta catttatgaa tctcagcggc
    35341 aaagccgttg cggcgatggc cagttttttc cgcattaatc cggacgaaat tctggtggcc
    35401 cacgacgaac tggatctgcc tcctggcgtc gccaaattta aattgggcgg tggccatggt
    35461 ggtcacaatg gactgaaaga catcatcagt aaattgggta ataaccctaa ctttcaccgt
    35521 ttacgcatcg gaatcggtca tcctggcgat aaaaataaag ttgtcggttt tgtgttaggc
    35581 aaaccgcctg ttagtgaaca gaagttaatt gatgaagcca tcgacgaagc ggcgcgttgt
    35641 actgaaatgt ggtttacaga tggcttgacc aaagcaacga accgattgca cgcctttaaa
    35701 gcgcaataag tcgttgtctg cggcattttt gccgagtgcc gtgtataata ggcaaagtta
    35761 tttccatttc tgcaatctgt tagcaataac agattgatta ttaagatatt aaggtgattt
    35821 aaatcatggg attcaaatgc ggtatcgtcg gtttgcccaa cgtcgggaaa tctaccctgt
    35881 tcaacgcgct gaccaaagcc ggtattgaag cggccaactt tccattctgc accattgagc
    35941 cgaacacagg cgtcgtacca atgcctgacc ctcgcctgga tcaactggct gaaatcgtaa
    36001 aaccgcaacg tacgcttccc acgaccatgg aatttgtcga tatcgccggt ctggtaaaag
    36061 gcgcatcgaa aggcgaaggt ctgggtaacc agttcctgac caacatccgt gaaaccgaag
    36121 cgatcggtca cgttgttcgc tgctttgaaa atgacaacat cattcacgtt tccggcaaag
    36181 ttaacccggc tgacgatatt gaagttatca acaccgaact ggcgctggca gacctcgaca
    36241 cctgcgaacg tgcgattcat cgcgtacaga agaaagccaa aggtggcgat aaagacgcga
    36301 aagctgagct ggcggtccta gaaaaatgcc tgccccagct ggaaaacgca ggtatgctgc
    36361 gcgcgctgga tttaagcgct gaagagaaag cggctattcg ttacctgagc ttcctgacgc
    36421 tgaaaccaac aatgtacatc gccaacgtca acgaagacgg ttttgaaaac aacccatatc
    36481 ttgaccaggt gcgtgaaatc gcggcgaaag aaggttctgt tgtggttccg gtttgtgctg
    36541 ctgttgaagc agacattgcc gaactggacg acgaagaacg tgacgagttt atgcaggagc
    36601 ttgggctgga agaaccgggc ctgaaccgtg tgatccgtgt cggttataag ctgctgaacc
    36661 tgcaaactta cttcaccgct ggggtgaaag aagtgcgtgc atggaccatt ccggttggag
    36721 caaccgcgcc gcaggcagcg ggcaaaatcc atactgattt tgaaaaaggc tttatccgtg
    36781 cacaaaccat ctcgtttgaa gatttcatca cttacaaagg tgaacaaggc gcgaaagaag
    36841 caggcaaaat gcgtgcagaa ggtaaagatt acatcgttaa agatggcgat gtgatgaact
    36901 tccttttcaa cgtctaatta atttattgtc tcatgaggtt tcgctaaatc tcatgaagag
    36961 cgcaaaagta tataaaatcc acgcaattgc gtggattttt catttcaccg agtcctaatc
    37021 attcttgtaa catcctacac ttttgtgctt attgcctaca gtgaaccacg agaataatta
    37081 aagacatcag tgtcagatga cggatatctc attagtagtg atataacgta agtttttgtt
    37141 caactacaca tcaccccctt cacagctgat caatattgat cacgctccaa tatgctggcg
    37201 agaaaaggac ttacctgaag ggtaatatcc cgcagataaa gtccccgaat aacgtgatta
    37261 cgtgcgatcc tggcaagatg aaaatgataa ccagtagcac aataacaacg ataggccaga
    37321 aaacccattg accaaaaatg aatatcgatg ttttttgctt tttcttatta gtaatgcaaa
    37381 aatggcgcat attgtttttg ttatgcagat acatcaagcg aaattcccat aactaattag
    37441 gattattaca aaaaatcttc accaaatttt ttaaccgctg tttgttgtaa tatttttgcc
    37501 atatttatag taacgaattg gaataagtaa cgacaactta gggaataact cgatataaac
    37561 tcacctttgt aagggggatg ttaaatatat attaattcct gtgttcctga ttttgcccgg
    37621 taatagagaa ttattctctg agaagctcat catgattgca ccatacaaat atcatgatga
    37681 taagaggata ttattcattg atttaaacaa aatgacaaaa tccatagacc catagtctgg
    37741 gtactatata ggagtggtat gggcatcaaa caacacaatg ggaataccaa agcagatcgt
    37801 ctcgctgaat taa
//
LOCUS       DAZPEP010000025        34772 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZPEP010000025 DAZPEP010000000
VERSION     DAZPEP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.025"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(210..3314)
                     /gene="acrF"
                     /locus_tag="ACHUT9_001235"
                     /pseudo
     CDS             complement(210..3314)
                     /gene="acrF"
                     /locus_tag="ACHUT9_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(3326..4483)
                     /gene="acrE"
                     /locus_tag="ACHUT9_001236"
     CDS             complement(3326..4483)
                     /gene="acrE"
                     /locus_tag="ACHUT9_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFR1347757.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4882..5544
                     /gene="acrS"
                     /locus_tag="ACHUT9_001237"
     CDS             4882..5544
                     /gene="acrS"
                     /locus_tag="ACHUT9_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFR1347758.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(5547..5726)
                     /locus_tag="ACHUT9_001238"
     CDS             complement(5547..5726)
                     /locus_tag="ACHUT9_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFR1347759.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5810..6694)
                     /gene="yhdJ"
                     /locus_tag="ACHUT9_001239"
     CDS             complement(5810..6694)
                     /gene="yhdJ"
                     /locus_tag="ACHUT9_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFR1347760.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6780..7076)
                     /gene="fis"
                     /locus_tag="ACHUT9_001240"
     CDS             complement(6780..7076)
                     /gene="fis"
                     /locus_tag="ACHUT9_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFR1347761.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(7102..8067)
                     /gene="dusB"
                     /locus_tag="ACHUT9_001241"
     CDS             complement(7102..8067)
                     /gene="dusB"
                     /locus_tag="ACHUT9_001241"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFR1347762.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(8396..9277)
                     /gene="prmA"
                     /locus_tag="ACHUT9_001242"
     CDS             complement(8396..9277)
                     /gene="prmA"
                     /locus_tag="ACHUT9_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFR1347763.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(9289..10740)
                     /gene="panF"
                     /locus_tag="ACHUT9_001243"
     CDS             complement(9289..10740)
                     /gene="panF"
                     /locus_tag="ACHUT9_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFR1347764.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10730..10972)
                     /locus_tag="ACHUT9_001244"
     CDS             complement(10730..10972)
                     /locus_tag="ACHUT9_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFR1347765.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(11081..12430)
                     /gene="accC"
                     /locus_tag="ACHUT9_001245"
     CDS             complement(11081..12430)
                     /gene="accC"
                     /locus_tag="ACHUT9_001245"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFR1347766.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(12441..12911)
                     /gene="accB"
                     /locus_tag="ACHUT9_001246"
     CDS             complement(12441..12911)
                     /gene="accB"
                     /locus_tag="ACHUT9_001246"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFR1347767.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13889..14863)
                     /locus_tag="ACHUT9_001247"
     CDS             complement(13889..14863)
                     /locus_tag="ACHUT9_001247"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFR1347768.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15015..16955
                     /gene="csrD"
                     /locus_tag="ACHUT9_001248"
     CDS             15015..16955
                     /gene="csrD"
                     /locus_tag="ACHUT9_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFR1347769.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17260..18303
                     /gene="mreB"
                     /locus_tag="ACHUT9_001249"
     CDS             17260..18303
                     /gene="mreB"
                     /locus_tag="ACHUT9_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFR1347770.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            18369..19472
                     /gene="mreC"
                     /locus_tag="ACHUT9_001250"
     CDS             18369..19472
                     /gene="mreC"
                     /locus_tag="ACHUT9_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFR1347771.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            19472..19960
                     /gene="mreD"
                     /locus_tag="ACHUT9_001251"
     CDS             19472..19960
                     /gene="mreD"
                     /locus_tag="ACHUT9_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFR1347772.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19969..20562
                     /gene="yhdE"
                     /locus_tag="ACHUT9_001252"
     CDS             19969..20562
                     /gene="yhdE"
                     /locus_tag="ACHUT9_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFR1347773.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            20552..22021
                     /gene="rng"
                     /locus_tag="ACHUT9_001253"
     CDS             20552..22021
                     /gene="rng"
                     /locus_tag="ACHUT9_001253"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFR1347774.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            22089..25889
                     /gene="yhdP"
                     /locus_tag="ACHUT9_001254"
     CDS             22089..25889
                     /gene="yhdP"
                     /locus_tag="ACHUT9_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFR1347775.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNAGLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            26045..27490
                     /gene="tldD"
                     /locus_tag="ACHUT9_001255"
     CDS             26045..27490
                     /gene="tldD"
                     /locus_tag="ACHUT9_001255"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFR1347776.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(27618..28547)
                     /gene="aaeR"
                     /locus_tag="ACHUT9_001256"
     CDS             complement(27618..28547)
                     /gene="aaeR"
                     /locus_tag="ACHUT9_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFR1347777.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            28730..28933
                     /gene="aaeX"
                     /locus_tag="ACHUT9_001257"
     CDS             28730..28933
                     /gene="aaeX"
                     /locus_tag="ACHUT9_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFR1347778.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28941..29873
                     /gene="aaeA"
                     /locus_tag="ACHUT9_001258"
     CDS             28941..29873
                     /gene="aaeA"
                     /locus_tag="ACHUT9_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFR1347779.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMDHRLREFG"
     gene            29879..31846
                     /gene="aaeB"
                     /locus_tag="ACHUT9_001259"
     CDS             29879..31846
                     /gene="aaeB"
                     /locus_tag="ACHUT9_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFR1347780.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31938..32210
                     /locus_tag="ACHUT9_001260"
     CDS             31938..32210
                     /locus_tag="ACHUT9_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFR1347781.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(32266..32529)
                     /gene="yhcN"
                     /locus_tag="ACHUT9_001261"
     CDS             complement(32266..32529)
                     /gene="yhcN"
                     /locus_tag="ACHUT9_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFR1347782.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(32894..33364)
                     /gene="argR"
                     /locus_tag="ACHUT9_001262"
     CDS             complement(32894..33364)
                     /gene="argR"
                     /locus_tag="ACHUT9_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFR1347783.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            33799..34737
                     /gene="mdh"
                     /locus_tag="ACHUT9_001263"
     CDS             33799..34737
                     /gene="mdh"
                     /locus_tag="ACHUT9_001263"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1347784.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
BASE COUNT         8495 a         8636 c         9075 g         8566 t
ORIGIN      
        1 ctcttattaa cgagctgaat gccattttat caccggaggt tataaatatt gcggaatgaa
       61 taatgacaaa ttatgtgaat tgtaaaaata gtgaagggag cgggaactat agaaagtttt
      121 taaagagtga gtagaagaat atattattaa tttttatggg tttcactgaa aataataaag
      181 gcacccgaaa gtgcctttat gtttctgatt tatcctttaa agcaacggcg gatcaccaca
      241 aagaacaccg gtacgaagaa gattgccagc aacgttgcag agaccattcc tcccattacc
      301 ccgataccca ctgcgttctg cgcgccactg ccggcaccgt tactgatagc tagcggtaat
      361 acgccgagaa taaaggcgag agaggtcatc aggataggac gcagacgcat acgtactgcc
      421 atcagtgtcg cttcaacaac acctttaccc tttttctcca tgagatcttt agcgaactca
      481 acaatcaaaa tagcgttttt ggccgacaag ccaattgtcg ttagcaagcc caccataaag
      541 tagacgtcat ttttttgatt aaagagtgtt gccgccagta gcacgccgac aatccctaac
      601 ggcacgacca acataaccga gacaggaatt gaccagcttt catagagtgc agcaaggcac
      661 aggaagacaa ccacaaagga aattgctacc agagcgggag cctggtttcc cgataagcgt
      721 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
      781 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      841 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      901 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      961 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
     1021 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
     1081 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
     1141 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
     1201 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
     1261 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
     1321 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
     1381 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
     1441 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
     1501 acgttcgctt tctcgttatt cagatagtaa tccgtcactt gatccaacac tttttgcgtc
     1561 cgctcttgcg tcgcgccagc gggtaactga atcatggtca gaaagacacc ctgatcctct
     1621 tcaggtaaga aggaagacgg aagacgtaaa aacaacacca ccattcctgc aacaatcagc
     1681 gcatagatca gtaaatatcg tcctgtggaa ccgaggattt tgccgacgct gttggtgtag
     1741 tggttaacgc tatgatcgaa ggtggtatta aaccagccga agaaaccgcc cttattttcg
     1801 tgatgctcag cagagacggg tttaagcagc gttgcacata acgcaggggt aagaatcaat
     1861 gccaccagaa cagaaagcgc cattgccgaa acgatggtga tagagaactg gcgataaatt
     1921 gccccagtag aaccgccgaa gaatgccatc ggaataaata ccgctgacag caccatcgcg
     1981 atacccacca gtgcgccctg aatttgcgac atcgattttt ccgtcgcttc ttttggcggg
     2041 agcttatcct ccatcatcac gcgctcgacg ttctccacca ccactatcgc atcatcgacg
     2101 agcagcccga tggcaagcac catcccgaac atcgttagtg tgttgatgga gtaaccaaaa
     2161 gcggcgagga tggcaaacgt ccctaacaac accacgggta ccgcaatggt ggggatcagc
     2221 gttgctcgca tattctgcaa gaacagatac atcaccagga acaccagcat aatggcttcg
     2281 aacagcgttt ttaccacttc gtgaatagaa agctggacga atggcgtggt gtcataaggg
     2341 tagagaacct tcattccctg cgggaagaat ggctgtaatt ccgccagttt tgccttaatg
     2401 gctttcgcgg tatcgagagc gttcgcgccg gttgctagct taatccccag gcccgccgcc
     2461 ggttttccgt tgatacgagc gataacgtta tagttttcac cgccaagttc aactcgtgcg
     2521 acatctttca ggcgtaccac cgagccgtca ctgtttacgc gcagggttac tttgccgaat
     2581 tcttccggat tttttaaccg cgtctgagca ataatcgagg cgttcaactg ttgccctggt
     2641 aacgctggcg ttccgcccaa ctgcccggca gcgatctgat cgttctgtac cttcaactgg
     2701 ttaatcacat caaccggtgt cagtttatat ttgtttagca gatcggcgtc cagccagata
     2761 cgcatcgcat actgtgcgcc gaaaagctgt acgtcaccga cgccattcag acggctaagc
     2821 gtatctttaa cgttagaggc cacatagtcc gagatatcgt cctgtgtggt gtctgggtta
     2881 tcagagacaa agcccgccac catcaaatag ctgctactgg acttttcaac actgatcccc
     2941 tgctgctgaa cctcctgcgg cagcaacggc gtggcgagct ggagtttgtt ctgcacctgc
     3001 acttgcgcga tatcaggatc ggtcccggac tggaaggtaa gggtaattgt cacgctaccg
     3061 gcggaatcgc tggtggagga catatacatc aggttatcga taccattcat attctgttcg
     3121 ataacctgcg tcaccgtatc ctgcacggtc tgcgcatcag cacctggata gtttgcctaa
     3181 acagaaaccg caggcggtgc aatggttgga tactgagcga cgggcaattg taggatcgcc
     3241 agtgcgcccg ccatcatcag aataatggcc agcacccatg caaatatcgg tcgtcgaata
     3301 aaaaagtttg ccatgtcaga ttaccttact tcgatgcagt atctgcgggg gtatcggtag
     3361 tggctttaac ctgctctccc ggacgcgctt tttgcaggcc gctgacaatg acttgatcgc
     3421 cagatttcag accttcgcta atcaaccatt tatcgccaat cgcctgactg gcaacgacag
     3481 ggcgcgcttc cacctgactt ttatcgttaa caatcagcac ggttgcatca ccacgcggtg
     3541 tgcggctaac gccttgttgc gggataagaa tggcgtcagg ttggacgcct tcatcaatcc
     3601 gtgcacgcac aaacataccc ggcaaaagcg tatgttgcgg gttagggaag acagcacgta
     3661 gggttatgga gccggtgcat tcatcaacgg tcacatcaga gaattgcagc gtacctttca
     3721 ggggataggt ttgaccgttt tccatgacca actctacgtt gctggtggcg ttttccttat
     3781 gcaaatttcc ttgctctacg gattgcttca gcctcataaa atcgttgctg gattgggtca
     3841 catcaacgta gataggatcg agctgctgga cagtcgccag ttcagtcgtt tgcccattag
     3901 tgacaagagc gccttcggtc acggtcgatt tgccgatacg tccgctaatt ggcgcagtca
     3961 ctttggtata agcgagattg atgcgagcgc tttcgactgt ggcttttgcg gcaatcacgg
     4021 cggcatcggc ctggcgagca tcagcaatgg cctggtcgta ctcctgctgg ctgatgtatt
     4081 tcgtacccac gagcggaacg taacgtttta ccgtcaaatg cgcgatggcg gcggcggctt
     4141 cacttttcgc cagttcgcct ttcgcgctgt cataatttgc ctgataggtc gcgggatcga
     4201 tctggtacag ggactggcct gcttgtacat cgctgccttc agtgaaatta cgattcagta
     4261 cgatcccgct aacctgtggg cgaacttcgg ctatacggta agcattggtg cggcctggta
     4321 attcagtctt aacttctaac ggggccgttt tcacaatatg aacggtaacc tgcggttcac
     4381 cgacgtgagc tttctcttct cccttatcgt tacaaccgac gattaaagcc gcggagatca
     4441 gaataaagga gggcaggagg aaaaacctgg catgtttcgt cattactatt cctcaaaaaa
     4501 acaaaagcgc gttatttacc cagaaaggca acgcgttaac tcgcagaaag aaaaatacag
     4561 tttgctatcc tacaaattat cattcgtcga tgtaaggaat agttatgaat actggcgtct
     4621 caaggcacat aaacacaaaa aaagattaat attctactgt tttattttga cgcgggttga
     4681 aagaggcaga attaaaacct cgtaaattga aatatatatt catgtggcga atgtatttta
     4741 ggtaaataat atatattatt tatttgacga tttatagcaa ctcaatgtta ccctctcgta
     4801 taaatacaca ttagatgata gattaacctt cgctattttc tcaccctgtg tcgaatatat
     4861 ttatttcctg aataattaat catggcaaaa agaaccaaag ccgaagctct gaagacccgg
     4921 caagaactga ttgaaactgc catcgcccag tttgcgcaac atggcgtaag caagacgacg
     4981 ttcaacgaca ttgccgatgc cgctaacgtt acgcgtggcg ctatctactg gcacttcgag
     5041 aacaagaccc aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc
     5101 caggaacact tgacggctgg attagagcat gacccgtttc aacaattgcg tgaaaaattg
     5161 attgtcggct tgcaatatat tgccaaaatt ccccgccagc aggcgttgct gaaaatctta
     5221 tatcacaaat gtgaatttaa tgatgggatg ctggccgagg gagtgatacg cgaaaagatg
     5281 ggctttaatc cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta
     5341 gcaaataacc tcgatttaga tgttgtgatg attattattg atggtgcctt cagcggaatt
     5401 gttcaaaact ggttaatgaa tatggcgggt tatgatcttt ataaacaagc acccgcgctg
     5461 gtcgataacg tattaagaat gttcatgcca gatgaaaaca taacgaaatt aattcatcaa
     5521 acgaatgaat taagtgtcat gtaaattcac attctgtcct gaaaattcag ttcgtcacag
     5581 ttaaccagtt ttagtggata aactgagttc atattacggc atttgtctgt ttctgttgtc
     5641 agcagttcaa tccactgcat cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg
     5701 acgagccacc aatacttgcg aatcattacc tgagcctgtg catgtttaac caagtctggc
     5761 aggaaatata cacgaaatga ataatctgaa aaccaggctt acacttcctt tactttgcaa
     5821 tgagatcggg gtcaacttcg cttaaccggc ttcgttttga caggttgccg gtctttcttt
     5881 ttttcacttt cgccagttct tccgcggagt aatgcgacgc gacatccagc cgtcgaagcc
     5941 ccattttgat gtactcgctg ttgatctcaa taccaatgaa ttttcgtccg ctggcgatgg
     6001 ctacggcacc ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg cctgggttgg
     6061 aagaggcgag aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg tggttctcat
     6121 attcatccat caaataacgc acgcgcggaa aatcccagac gttacccggt actttttgat
     6181 gattgtatgg ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt
     6241 tggcttctac cagaatagca tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca
     6301 tcaggatggg ttcgtacatg gagccgtagt gttttttcgc ctgtactcca gaactgtcat
     6361 atgaccagac gatgcgactt ttgatggtaa aaagcttgcg gcactggaga tcgataaagg
     6421 gcatgttttc cgtactgttc ataatgtaca tgctgccctg ctttttcaga acgcggtggc
     6481 actctacaat cacttcaaac agccagtcga taaacagatc ttctttccag gcttcgatca
     6541 gaccatcaaa atttttaccg atgttatacg gtgggtcggc aaagatcaga tcgacgcttt
     6601 cagtaggtag ctttttaagt tcggcaaggg catcaccgtg aataatggtc ttagcttcat
     6661 taccaaaccg ggtcggttca catcctgttc tcatggtcac tccctttgtg acacctataa
     6721 aaaaggcgct tccccatgcc gagtagcgcc tttttaatca agcatttagc taacctgaat
     6781 tagttcatgc cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc
     6841 agcgcagcac gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt
     6901 tctacttcag ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca
     6961 aaatagttct tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc
     7021 tgagagttaa cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt
     7081 ctgtcagctc tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag
     7141 ctgttcgctg gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg
     7201 ttcctggaga taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata
     7261 aaagtcatgc agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag
     7321 cgggggcagc aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct
     7381 tccctgagct gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct
     7441 ggctttaagc gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt
     7501 aactgcccga atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc
     7561 atgaatggtc agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt
     7621 acggtgttcc ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt
     7681 gacgacctcg gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc
     7741 gagcttgcga ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc
     7801 gctttccacg ttaatacgtg ctgcatctgc catttctttc ggatcgctac cagcaatttg
     7861 cacggtgcga ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc
     7921 ccaaacctgt gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta
     7981 gcacaacgtc cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt
     8041 tctgagctga tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg
     8101 cggcgtatat tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga
     8161 gatggatcaa agaattgcat ttaaaatgag cgtggtgaga taattactca taaaaatcat
     8221 catattagaa aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt
     8281 ttctttatga atgaaaattt tggcacgcaa atttgcgtaa ataaccggca aatttacgtg
     8341 ccttttgtga gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc gaaggttaat
     8401 tcttacgacc ggtaatgcgg caccactctt ctttttccac gaccgggtcc agtgcgaagc
     8461 tgtcgacata agcttcacaa acgctctctg cctggcttgc cagaataccg gaaaggccca
     8521 gcaaaccgcc tgaaaccggc aggacgctga ttaacggtgc cagttcacgt aatgggcctg
     8581 caaggatgtt agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt
     8641 agagttccag acggtcagaa acgccattac gttcggcgtt gtcgcggcta gcctgaatcg
     8701 cctgcggatc gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatag
     8761 ccagaatgcc ggaaccacag ccaaagtcga tgactatttt accggttaaa tcgaggctgt
     8821 cgagccactg caggcacaga gaggtagttg gatgggtacc cgtaccaaac gccagccctg
     8881 gatctaacat cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc
     8941 acagtcgttc accaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt
     9001 tatcttccag ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca
     9061 gaatcgccac cacgtcgttc atatcggttt cagcgtcgaa cagaccaatc acatcggtgt
     9121 cgccccacag gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct
     9181 gaaaagtgat agaaacggca cccgcttcca tcagcgcatc gctaagatct tcagcgttcg
     9241 cgccggtggt gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt
     9301 agtcaaaacg gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag
     9361 caaacttagt agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt
     9421 cgcgagtacg gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt
     9481 ggcgcgttcc cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc
     9541 gaaggccagc aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag
     9601 caacgcgccg agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg
     9661 catttgatcc ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgtag
     9721 caattgggcg ttaattgtcg acatgatcgc agccataggt gcagccagaa agatcccggc
     9781 agcaaacggt ggcagcactt ttaccattaa cgtcgggatc accagatccg gtacggtgag
     9841 atcggggatc actgcacgac ctaacgctcc ggctaggtgc ataccgaaca tcaggattgc
     9901 gactacaatc gtaccgatga tgatcccccg gtgtaccgct ttgctgtctt tataagagat
     9961 acagcgcacc gcagtatgcg gcaggccaat cacgccaaag cacaccagta cccagaacga
    10021 cgtcataaag gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc
    10081 gatggtttgc aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat
    10141 aagcagcaca acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct
    10201 ggcgcgaaag ccgccaaagg cggtatacaa cgcaatgctg ataccgaaaa tcagcagccc
    10261 ggtttcataa ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac
    10321 ggtcattgcc ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg
    10381 actctggtag cgggcaaaca gcatgtcgtt cagcgtcact gcattgtagc ggcgcgcaag
    10441 aatcgcaaac ttcttgccga gaataccgag tgaaagccag actgcaggaa gctgaatcat
    10501 cgccagcaat acccagccca gcccgtattt ataagcagct cctggcccgc cgataaacga
    10561 actggcactg atataggtcg cggtgagcgt catcgccagc acaataccgc ccatagagcg
    10621 gctgccgagg aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac
    10681 cgagataccg aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat
    10741 cgtcatcctc cagtgggata tcgcgataga taaatttcac catcgcccag cacagtccaa
    10801 taaacagtag cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac
    10861 cgggggcaac gccaggtaag taagcggcta ctaaccaaac tgccagatac aaaagggtca
    10921 gccccagcgc ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc
    10981 cctgatgggt gaagaaagcg aggattgtac cttatggggg ttgtcgatcc ccagtaataa
    11041 aaaaggccgg aaaatccggc cttttgacgc ttttgcagtc ttatttttcc tgaagaccga
    11101 gttttttctc cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga
    11161 tgcggatctg cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat
    11221 tcttcatgcg ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca
    11281 ttgagtcata gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgtacgc
    11341 caaaaccgcc aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg
    11401 gatcttcggc gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct
    11461 tgatcgacag cggttgaccg gcagcgatac ggagctgttc tttgatcagg tcaacgccag
    11521 tgatcatttc ggtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga
    11581 actcgccgtt ttcgaacaga aactcgaaag tacctgcacc gcgatagccg atatcaacac
    11641 acgctttagc gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg
    11701 gcgcttcttc gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat
    11761 agatagcgtt gccctgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca
    11821 ggtatttctc catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca
    11881 tggagatgga ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc
    11941 cgccgccgga ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt
    12001 tatccatatc gtcgcccaac gggccgtcag aacccggtac gcaagggacg cccgcttttt
    12061 tcatcgccgc gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc
    12121 cgatgaagat aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga
    12181 agccgtaacc cggatggatt gctactgcgc cggtgatttc agcggcgctg atgattgccg
    12241 ggatgttcag ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca
    12301 gtaatacgtg ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca
    12361 gttctttaca ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt
    12421 tatccagcat gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt
    12481 tgtccacttt cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc
    12541 atcattttca tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact
    12601 tcgatgaacg cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggaa
    12661 cgtacgatgt gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc
    12721 ggagcggctg cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc
    12781 atcacaggga aacttgcggc aggagctgca cggctaatgc gtactgactc ttcgccttca
    12841 gaaatttcca gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta
    12901 cgaatatcca tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac
    12961 cgccgtctgt aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc
    13021 gggacgccgg tctattttgc cccagagtat caatgttaga cttgacggac attgtgcagc
    13081 gatctgctgc tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt
    13141 ttccgcagcg caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag
    13201 caattggcag ctaaatactg gtctcatcag ggaagataat caacagctaa catgtaaata
    13261 accttcaaca ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc
    13321 gacggaactt cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg
    13381 gctgcaatga gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa
    13441 gatagacgac attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga
    13501 tagatgtgaa tgctaaagca cgcaaaatca accgactgac aattctcagc attaaatagg
    13561 ttcgagtggt taatttgtag tcaaataaaa tcagattatt caccccatgg taccagaaat
    13621 cagtaacagg ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg
    13681 aggcgtatca ataatggctg tttagaggag tatcaatgcg catcaatttg tggtcagtcg
    13741 aaatcatgac tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga
    13801 aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt
    13861 ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga
    13921 tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg
    13981 ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc
    14041 ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa
    14101 atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc
    14161 cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg
    14221 ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa
    14281 tacggctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa
    14341 cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg
    14401 cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca
    14461 cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc
    14521 cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc
    14581 agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg
    14641 gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac
    14701 ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt
    14761 gaacatcgac cgtgacatcg ccctccggca ggcgactttc gtccagagtc tgtactgatg
    14821 cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc
    14881 atgatggatt ggaaaataat ttctgaagat tatactcgct aatggaaaag caaagccgat
    14941 gaagcgcaat aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg
    15001 gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg
    15061 ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt
    15121 ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga
    15181 atgacttcag cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc
    15241 gtgtagacct gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc
    15301 gtccggttgg ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg
    15361 ggatgtcgct gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga
    15421 ctaccgcgcc gctcacgggg gcgattggct ttatcattgt tatgctcttc ctggcggtac
    15481 gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct
    15541 taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca
    15601 gcagtgcgct agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc
    15661 ttgatacgct gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac
    15721 tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg
    15781 ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatact tgggggcaca
    15841 gccaggttga agaacagttc ttcactctga ctaatctgct gtcgacattt atgatgcgct
    15901 accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc
    15961 ggacgttaaa agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc
    16021 cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta
    16081 gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat
    16141 tgcagggcgg caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta
    16201 atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc
    16261 aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct
    16321 tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct
    16381 tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc
    16441 cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc
    16501 gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg
    16561 aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag
    16621 tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca
    16681 gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca
    16741 ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga
    16801 ccagcaccca cgtttacgcc accggcgtgc gttcgcgaag cgagtggcag accctgattc
    16861 agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta
    16921 acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt
    16981 agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc
    17041 acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat
    17101 taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc
    17161 accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga
    17221 ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc
    17281 atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa
    17341 ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt
    17401 tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg
    17461 ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact
    17521 gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc
    17581 ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt
    17641 gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc
    17701 gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt
    17761 ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg
    17821 cgcattggtg gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt
    17881 tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg
    17941 ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc
    18001 ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt
    18061 gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc
    18121 gagcgcggca tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta
    18181 atggaagaaa ccggcattcc agtagttgtt gctgaagacc cgctgacctg tgtggcgcgc
    18241 ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag
    18301 taatcggatg caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg
    18361 cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg
    18421 cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa
    18481 tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg
    18541 aattgctgga tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc
    18601 gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca
    18661 aacaggagaa cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga
    18721 aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg
    18781 ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg
    18841 ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga
    18901 cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg
    18961 gttgtacgga tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg
    19021 atgtgctggt gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg
    19081 tctcttccgt aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg
    19141 caggactgca acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta
    19201 acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc
    19261 cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga
    19321 tcgctcagcc gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgcgc
    19381 ccacacagcc tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc
    19441 aaccgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggacgttg
    19501 ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa
    19561 cctgattgtt ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt
    19621 gcctcatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag
    19681 cggctcgacg cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct
    19741 gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct
    19801 ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt
    19861 cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt
    19921 gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg
    19981 tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg tgtgaccttt
    20041 gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat
    20101 gttgtgcgtc tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc
    20161 ccggtgctgg gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc
    20221 gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg
    20281 acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg
    20341 actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta
    20401 gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat
    20461 ggcagctatc acgccgtagt cggcttacct ctggttgaaa cgtatgaatt attaagtaat
    20521 tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac
    20581 gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga
    20641 acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact
    20701 tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc
    20761 atccgatatc atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt
    20821 gcgcgacatc tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga
    20881 tccgcttggc actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct
    20941 ggtctttatg ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga
    21001 acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat
    21061 ccgtaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa
    21121 acgcgtctgg accaaagtta tggagcgtaa aaagcgcccg cagacccgtt atcagctgta
    21181 cggcgaactg gcgctggctc agcgtgttct gcgtgatttc gccgacgccg aactggaccg
    21241 cattcgcgtt gactcacgcc tgacttacga agcattactt gagttcacct cggagtacat
    21301 tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt
    21361 tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg
    21421 ttatctcatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt
    21481 tgtcggtcat cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc
    21541 tatcgctcgc cagttacggt tgcgtaatct gggcgggatt atcattattg atttcatcga
    21601 tatgaataat gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa
    21661 agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg
    21721 taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg
    21781 tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg
    21841 tgtccaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg cagtagccga
    21901 agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca
    21961 ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta
    22021 aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg
    22081 agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga
    22141 tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc
    22201 cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct
    22261 cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa
    22321 aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc
    22381 tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca
    22441 acactcctat caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc
    22501 tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt ttcctgacgc
    22561 catccggtca gcgtgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc
    22621 gacaccgtgc ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc
    22681 aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac
    22741 aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc
    22801 tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg
    22861 gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata
    22921 cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct
    22981 ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc
    23041 tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc
    23101 ggattcgcgc cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga
    23161 aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca
    23221 ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga
    23281 gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc
    23341 tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt
    23401 acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc
    23461 tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg
    23521 tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta
    23581 ttctggctgg catcagtacc gatgatggtt cacaagcctg gcactatttc ccggaaaact
    23641 tgatgggtaa agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata
    23701 acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc
    23761 agtttgaagt gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg
    23821 cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg
    23881 atggcgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact
    23941 cgaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt
    24001 actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg
    24061 acggcgatgt gaatgctggc ttacatcttg atatcccgct gaacggcgag ctggtaaccg
    24121 cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc
    24181 tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga
    24241 cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag
    24301 cctaccaggt tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc ggcgttctgc
    24361 ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg
    24421 atctgcctta tcatgctggc gcgacctata acgtagagct gaacggcgat ctgaataatg
    24481 tgagtagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg
    24541 ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc
    24601 atttcaatag ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg
    24661 cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc
    24721 cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg
    24781 gtgcagcgag tttcccacaa tacataacgt tacgtacgcc tatgttgtca ctgggaaatc
    24841 agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg
    24901 cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga
    24961 atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt
    25021 catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac
    25081 gatgcacaga gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca
    25141 tttctggcga tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta
    25201 ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg tacctcgctg aaaggaaaac
    25261 tgcgcgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc
    25321 agtcgtcatt taatgtggat tacgatttac actggcgcaa agcaccctgg cagccagatg
    25381 aagcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt accgaaatca
    25441 ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg atgcgtaagc
    25501 tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca
    25561 ccgcgtggat taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg
    25621 cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg
    25681 ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca
    25741 ttgttggcgc ggcagttttt gccgctagta aagtgctggg gccgctgtgg agcaaagtct
    25801 ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac gaagtgttgc
    25861 gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc
    25921 atatgataat cgttgccaaa ggccaacgag ccagaacata accgtaggcc ggatagggcg
    25981 ttcatgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa
    26041 aacgatgagt ctgaacctgg taagtgaaca attgctagcg gcgaacagcc tgaaacatca
    26101 ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta
    26161 ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc
    26221 ttacaacatc gatcagggcg ttggcgtgcg tgcaatcagc ggtgaaaaaa ccggatttgc
    26281 ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat
    26341 cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt
    26401 gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg
    26461 tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta caggaagtga ctgccagcct
    26521 cagtggtgtc tatgaattaa ttctggttgc ggccaccgac ggcacgctag cggcggatgt
    26581 ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg
    26641 cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg
    26701 cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc
    26761 tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt
    26821 gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc
    26881 agtatttagt gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga
    26941 cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca
    27001 gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc
    27061 gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc
    27121 catgccgcgt atgactaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat
    27181 tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatatcac
    27241 ctccggcaaa ttcgttttct ccacttcaga agcatatctg attgaaaacg gtaaagtaac
    27301 gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc agcagatttc
    27361 gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca
    27421 aagtttgccg gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttggcgg
    27481 tactgcgtaa taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg
    27541 gtcccctcgc tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg
    27601 taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta
    27661 ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc
    27721 ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc
    27781 aggatctcca gctcaccgcg attgatctcg ttgatcaccc acatcagcgg cacgtaggcg
    27841 atcccggcac ccgccgtcag ccagcgcacc agcgtcatcg gatcattagt cacaaatctt
    27901 ccttgtggga tcaggcgagt cgagatccct tccggtgcga tcagttcaaa ttcattgtcg
    27961 ggccgcacgc tgtattcaag ccatgaatga ctactcaaat cggcgggttt ttccggtatg
    28021 ccgtattgtg tgagatagct tttcgcggcg cacaccacca tcggcatcgc gcccagacgg
    28081 cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg
    28141 gcaatcaggt cgggggctgg aattccggta accagattga cgctcaaacc tgggtattct
    28201 ttcagcattt ttgctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca
    28261 atgcgcagcg tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc
    28321 acttcatgaa gcatccgacg gcagccctgg tagtaaattc tgccggcttc ggtcaggcca
    28381 atgctgcgtg tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc
    28441 gtctgactga tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca
    28501 aattcaacta ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac
    28561 ttaaaagtga tttagatcac ataatataga taacagcata acagttacgc taatatatta
    28621 aatatcaatc tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac
    28681 agatgctgaa aataataacg cctgctctct ctttacaacc aaggtcaaca tgagtctgtt
    28741 tcccgttatc gtggtgtttg gactgtcctt cccaccgata ttttttgaat tgcttttatc
    28801 actggcgatt ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt
    28861 ctggcatccg gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg
    28921 actgttcgtt tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca
    28981 cggtcgtatt agtcattcta gccttcatcg caatttttaa tgcctgggtc tattacaccg
    29041 aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg
    29101 tttctggact cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga
    29161 tactgttcac catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg
    29221 ttgcttatta tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc
    29281 tcggtgtgca ggcgatgtct cgcgaagaga tcgaccaggc caacaacgta ctacaaacgg
    29341 ttctgcatca gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac
    29401 gcacggtgat ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg
    29461 agtttattac tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac
    29521 tggcctatat ggaagaaact aagctggaag gggggcgtcc ggggtatcgt gcagagatca
    29581 cgccgcttgg cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gcaggggtca
    29641 ccaacgccag cagcacgcgt gacgacaaag ggatggcgac tatagactct aatcttgaat
    29701 gggtgcgtct tgcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca
    29761 tctggcctgc gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa
    29821 gccaggattc gttcttccgt aaaatggacc atcgcctgcg tgagtttggt taatcacgat
    29881 gggtattttc tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat
    29941 tgtactggcg ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact
    30001 gacagcggcg attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg
    30061 cgctattcgc tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg
    30121 actggtgatc atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat
    30181 ctgggccggt ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg
    30241 ggggctggcc ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct
    30301 tacgccgcag tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat
    30361 tatggcggat ttgctctttt ctccgcgatc gatcaaacaa gaagtagatc gagagctgga
    30421 aagtttgctg gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga
    30481 agttgtcgat aaagcctggg gcgacctggt gcgacgcacc acggcactac aaggcatgcg
    30541 cagcaacctg aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat
    30601 caatacgcta tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac
    30661 gcgcccggaa ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc
    30721 gcaggacgtc cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ccggggaacg
    30781 ggaaacgcct gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa
    30841 gcgcggcgtt atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga
    30901 accggaagta aaagtagagt cagccgaacg tcatcatgca atggttaact tctggcgaac
    30961 cacactttcc tgcattctgg gcacgctttt ctggctgtgg acgggctgga cttccggcag
    31021 tggtgcaatg gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg
    31081 catggtggcg atcgacttta tctatgggac gctggccgcg ctgccgttag ggctgctcta
    31141 ctttttggtg attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc
    31201 agtgctggga ttcttcctcg gtatagaagt acagaaacgg cgactgggct cgatgggggc
    31261 actggccagc accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca
    31321 gtttctcgac agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt
    31381 gctggtgcgg gataaatcgc gcgacaggac tggacgtgta ctgcttaatc agtttgtttt
    31441 tgccgctgtt tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc
    31501 actttatcag cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg
    31561 cctggcgctg acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa
    31621 cgaggattta tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc
    31681 ccgtagcgat gataaacgtc gtcggtactt tggccagttg ctggaagaac tggaaatcta
    31741 ccaggaaaag ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct
    31801 ggcggggatg ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc
    31861 caaaagcgtc ggttttttca tggctatact tagcgatgca cggcagaact cgccgcgaaa
    31921 cgtgacggtg gcaacagatg aatatttata cctttgattt tgatgagatt gagagtcagg
    31981 aggattttta tcgtgacttt agccaaacct ttggtctggc taaagataag gtacgcgatc
    32041 tcgactcact atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat
    32101 ttgttcatct gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg
    32161 aggcagagga agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa
    32221 agcccccgaa ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc
    32281 agccgtagcg tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc
    32341 tttctcttcc gcttttttgt tcagcatttc acgcatatcc attggcgaag acgccacacc
    32401 acttacggat acggtcccga ttgcttcacg attttgtgct tgtgcagtat caatggagtc
    32461 ggcagcgaat gcaccgaaag agagaacaga aagtacgctt aatgcagcta cagtggtttt
    32521 gattttcatg atttttacct cgacataatc ttttagctgg gcctttgttt cgtgacccat
    32581 atcacaaaat caagcataca ctaattattg aactaattaa taccacgcta actatttcag
    32641 ttgatactaa gtgttaaaaa tgtgaatttt aataacaaaa aagaattaaa aacacggtta
    32701 tttattgtta attctagtat tttcaattag ataatgaatt ttacataatg tgcttttttt
    32761 accgtatgta ttcattgtgt gaatgatatg tcgcggtaaa acgcactatt cgttaaatta
    32821 atcgcagggg aaagcagggg gttgagagga ataagcaaca ttttccccgc cgccagaagc
    32881 gacggggcag agattaaagc tcctggtcga acagctctaa aatcgcttcg tacaggtctt
    32941 tgacggtgaa accgttagca ggggtggtaa agatggtgtc atcgccagcg atggtgccca
    33001 gaataccttc tgctttgccc agtgagtcca gcaggcgagc aattaactgc gccgcgccag
    33061 ggctggtatg aatcacgaca actgcatcgt tgtagtcgat atccagcacc agattcttca
    33121 atggactgga ggtggttggt acacccagtt cagctggcag gcagtaaacc atttccattt
    33181 tggcattgcg tgtacgtaca gcaccaaact tggtcaacat ccgcgagact ttagactgat
    33241 taatattgtc aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg gagctaaatt
    33301 tctcttcttt aagtaatgct ttaaatgctt taactagttc ttcttgctta gccgagcttc
    33361 gcataagtca cccgatatgg tggttgatac aacattattg tgcatacaga tgaattttta
    33421 tgcaaacagt cagccctgaa gaaggctgaa ataatgttat gaaagagcgg gattttatca
    33481 aatttcgtta ttgagaaaca tgcctgcgtc acggcatgca aattctgttt aaaagtaaat
    33541 taattgttat caaattgatg ttgttttggc tgaacggtag ggtatattgt caccacctgt
    33601 tggaatgttg cgctaatgca taagcgactg ttaattacgt aagttaggtt cctgattacg
    33661 gcaattaaat gcataaacgc taaacttgcg tgactacaca ttcttgagat gtggtcattg
    33721 taaacggcaa ttttgtggat taaggtcgcg gcagcggagc aacatatctt agtttatcaa
    33781 tataataagg agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg tattggccag
    33841 gcgcttgcac tactgttaaa aacccaactg ccttcaggtt cagaactctc tctgtatgat
    33901 atcgctccag tgactcccgg tgtggctgtc gatctgagcc atatccctac tgctgtgaaa
    33961 atcaaaggtt tttctggtga agatgcgact ccggcgctgg aaggcgcaga tgtcgttctt
    34021 atctctgcag gtgtagcgcg taaaccgggt atggatcgtt ccgacctgtt taacgttaac
    34081 gccggcatcg tgaaaaacct ggtacagcaa gttgcgaaaa cctgcccgaa agcgtgcatt
    34141 ggtattatca ctaacccggt taacaccaca gttgcgattg ctgctgaagt gctgaaaaaa
    34201 gccggtgttt atgacaaaaa caaactgttc ggcgttacca cgctggacat cattcgttcc
    34261 aacacttttg ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt gccggttatt
    34321 ggcggtcact ctggtgttac cattctgccg ctgctgtcac aggttcctgg cgttagtttt
    34381 accgagcagg aagtggctga tctgaccaaa cgcatccaga acgcgggtac tgaagtggtt
    34441 gaagcgaaag ccggtggcgg gtctgcaacc ctgtctatgg gccaggcagc tgcacgtttt
    34501 ggtctgtctc tggttcgtgc actgcagggc gaacaaggcg ttgtcgaatg tgcctacgtt
    34561 gaaggcgatg gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg taaaaacggc
    34621 gtggaagagc gtaaatctat cggtaccctg agcgcatttg aacagaacgc gctggaaggt
    34681 atgctggata cactcaagaa agatatcgcc ttgggcgaag agttcgttaa taagtaattg
    34741 attagcgaat aataaaaaac cggagcacag ac
//
LOCUS       DAZPEP010000026        34316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZPEP010000026 DAZPEP010000000
VERSION     DAZPEP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.026"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(404..1030)
                     /locus_tag="ACHUT9_001264"
     CDS             complement(404..1030)
                     /locus_tag="ACHUT9_001264"
                     /inference="COORDINATES: protein motif:HMM:NF018697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1347785.1"
                     /translation="MSKTSDDRTFHYNQIVRFSERLEKAMGQISKSELARRSGLSEAS
                     IRKYLKGGSYPTIDSAAKVADACGVSLNWLLTGEDDSDSHTYKQPISNPKDEPLADPT
                     ATLVSLLSFVGSDERQALATLACEIGIKGILDRTQHIQSQPNSVEEAIRALNIRESLK
                     DAFCMALAGDEYTDREILRGIESRARERSPDGSQIPTTPDDDSVKKTA"
     gene            1211..1483
                     /locus_tag="ACHUT9_001265"
     CDS             1211..1483
                     /locus_tag="ACHUT9_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1347786.1"
                     /translation="MQLSKQDLTDLFIRPGQDWSAKAIYAALDNKGADMAALERAQDL
                     KPGSMRNVFYRPCPGYERVIAGAIGVEPYVIWPSRYQNKSTRDVAA"
     gene            1494..3539
                     /locus_tag="ACHUT9_001266"
     CDS             1494..3539
                     /locus_tag="ACHUT9_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1347787.1"
                     /translation="MWVTAQQCLGLAGFPAAEKNVRARLDKLTEQNPEWKRKRKGTKA
                     FEYYIECLPAEAQRILKKKYTQQLMNDASVPAVVVAKPSKQIAMRQELAVMNKCSEIA
                     LQAVQSLTDKQKAIADARILLAAEVHKLREYAGMTRTAAVQHIVDGVRCQALPERIIT
                     AANTANARQGTRTGVSKGSLQEWYSTYLLAQGNAIQLQALLAPGHHKEVPWEQVAWLN
                     EFFMFYRVWTRPTLQQAYRQFAEWWNDTYAHNPDLLAALPSVHAVRRVLGKVPKIVKE
                     RFRYTGSSWRSLNPFVRRDWSTLPVNGVWVGDGHCMKLTAFSPDGNIARPEATFIMDA
                     GQRFIVGWSLSLSENVIAVADALRHGMSQHGVPLIYYSDNGGGEKNRQLDADITGILP
                     RLGVEHHTGIPGNPQGRGIIERFNKIVPKDVAISFQSYCAKEADGESVRLQQRIAQSA
                     LKATHKGKDLTKRQVEAMGKIPTWEQLLSAIEQEVHRYNNRPHTSLPRRDNGEHYSPA
                     AYRKKLLAEQGAEIDYLSPEELHEMYRPEIIRKTERGEVRLFNNIYFSHALATEAGNE
                     VRVCYDIHDASSVIVRRMDGAFICDAIWNGNKVDAFAKPVIERVKQQRVKGRIARATA
                     VIEDAKRELRPALEQSVPEFDLGYMQQHLPHEREAEELYLFAADRDASLKKSGTHNK"
     gene            3554..4447
                     /locus_tag="ACHUT9_001267"
     CDS             3554..4447
                     /locus_tag="ACHUT9_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1347788.1"
                     /translation="MDLVEQLKDVMERRGYSQTQVGRAIGRSGAVINQFLQGKYTGDN
                     ADLELRIEGFIARELEKQKAKRITARFIETSTARRGLEVLSYAHQESEMCVIYGAAGV
                     GKTMILREYAARNRDVLLIEADPGYTARTLLGELCRQLGLKVRGNIHELIDACVRELR
                     GSARLVMVDEAELLPYRALEVLRRLHDKSGVGVVLAGMPRLLINLKGRSGEFAQLYSR
                     VALGLNLDDSFSRDDFNALAYGMMPEADDIEISNALYERSKGNPRRLFKLARGVYRIC
                     DISRASVSVKAIDKFAEMLIH"
     gene            4461..4799
                     /locus_tag="ACHUT9_001268"
     CDS             4461..4799
                     /locus_tag="ACHUT9_001268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347789.1"
                     /translation="MSAVVTSLSAEKRVLRSFSSNVVFERFPSIKHYVSLGSARGTVY
                     LNKYDLMRDLAGVDDAISWTIEEHDMLMTEYRFTNRDEFTLIWRLARQELHRTMKIAR
                     LMGRHSLISK"
     gene            5142..5426
                     /locus_tag="ACHUT9_001269"
     CDS             5142..5426
                     /locus_tag="ACHUT9_001269"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347790.1"
                     /translation="MSIEVKISIKEDDGGLGVSVRAKHDGEITKEEAKFSLILSKVIN
                     ASVLNIYKGHVVTKEQNGLFNTSEEQKEIIEKMLRGTLEQQDEKSKPTVH"
     gene            5445..5969
                     /locus_tag="ACHUT9_001270"
     CDS             5445..5969
                     /locus_tag="ACHUT9_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1347791.1"
                     /translation="MAMKTKRIKSAASLYVPQSKEDVISDIKKIGDLQRELERTETMM
                     NDAIGDITEQHAPMIEALKKDIDVLSKGVQSWCEAHRDELTQNGKTKTATLITGKVEW
                     RNRPPSVAVRGVDAVLETLRRLGLERFIRTKEEINKDAILNEPKAVEGVAGISVRSGI
                     EDFSITPFEQNTGV"
     gene            6001..6165
                     /locus_tag="ACHUT9_001271"
     CDS             6001..6165
                     /locus_tag="ACHUT9_001271"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1347792.1"
                     /translation="MAYAVGAARAERFKNWDIAADLWEKAALRPVNAHQHEWATRRKA
                     FCENAYLKGW"
     gene            6268..6975
                     /locus_tag="ACHUT9_001272"
     CDS             6268..6975
                     /locus_tag="ACHUT9_001272"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFR1347793.1"
                     /translation="MSKEKQIDKIKKLLNKARNNSSAKEAAAALRMARKMMQEIGLQE
                     NEIELLDITLSDSQSSPSNAHKAPHYVAILMSAVSRAFGVKALTVWKRFDDGSSKRSV
                     QFYGPRERPEIAAYAFDVLVRQITKARKEFLAGLHKNIKTANKTARADAFCEYWVMGV
                     YSNLEEFACTPKEEALIAEYEQRHFEHAKAFSGRKAKEVRGVDEARCKGFGAGRRVKL
                     NHAVTGKASTSHQLEAR"
     gene            6977..7285
                     /locus_tag="ACHUT9_001273"
     CDS             6977..7285
                     /locus_tag="ACHUT9_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFR1347794.1"
                     /translation="MSVVYIAGPMSGIANFNRAAFDIAAQALQREGHSTLNPATLPDG
                     LSQRQYMQICLGMLFCADAIYLLDGWIHSAGARAEFALAEKLGLDIHYQTQDMGVIHG
                     "
     gene            7278..7646
                     /locus_tag="ACHUT9_001274"
     CDS             7278..7646
                     /locus_tag="ACHUT9_001274"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347795.1"
                     /translation="MAKSPAERKREQRERERAAARHAEISGHRRIVLTVSEHTFQQIE
                     MARHARRPGAEPYGIDEYFELLAVEDTRRLQKQMAELSQRQCSLCGDTLPGDAQGCIR
                     RGEHGCGQDAVRRLLMLKTM"
     gene            7716..8120
                     /locus_tag="ACHUT9_001275"
     CDS             7716..8120
                     /locus_tag="ACHUT9_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFR1347796.1"
                     /translation="MMKRLIGAVKAGQAFLGWDDSIYRAVISRLTGGKTSAKACSVQE
                     LSTIKEYMHEQGFPRKPPKNKGRRPNVARDRKAILSKIEALLADAGRSWKYAEGLAAH
                     MYGQHVLEWLTYEQLFGVMVALAKDARRRNAP"
     gene            8425..8754
                     /locus_tag="ACHUT9_001276"
     CDS             8425..8754
                     /locus_tag="ACHUT9_001276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347797.1"
                     /translation="MNALAAYMLYCVVCSLKVKRKPYCIMSVVRRSMFPAVIEHFERH
                     VMHAFSWILQKNKNQVAHCAKQWPFYALDMDLVTGMGGDFSGSNLTANNYQAPHKRGF
                     LINRGEK"
     gene            8869..9120
                     /locus_tag="ACHUT9_001277"
     CDS             8869..9120
                     /locus_tag="ACHUT9_001277"
                     /inference="COORDINATES: protein motif:HMM:NF027406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFR1347798.1"
                     /translation="MNGNTTSIGSYVMSFITVVISLFRPEEWMVIGIVVGILSAIFTA
                     AVNYFFRAKEHRLRREEHQMRERYYRRHLHSNEHSGEQP"
     gene            9117..9647
                     /locus_tag="ACHUT9_001278"
     CDS             9117..9647
                     /locus_tag="ACHUT9_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1347799.1"
                     /translation="MSMRKTVGGAVCSIAVIAGLVVELYPADIRTSKEGLMLIGNAEG
                     CRRDPYKCPANRLTVGVGSTRDVTPGKRYTDEEIAQLWVRDIKDAEDCVNRYFNGKHM
                     PQRAFEAMTSLVFNVGCYGVRWNRKKNQATQIYREAQAGNWSAMCYRMTDFSYSAGKP
                     ILRPRREEEVAWCLGK"
     gene            9644..10210
                     /locus_tag="ACHUT9_001279"
     CDS             9644..10210
                     /locus_tag="ACHUT9_001279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347800.1"
                     /translation="MSRVSICLCIALLAAVCGFKHVLQQRESAINALNTEVAQLKARN
                     TTLTQERDQARAALSLQAKQINRANQVAEEARQARQKAELRAEKIRQSLHTGISTYAC
                     SNQLLPADDAERLLRYVTHLRDEALHPDAKRTARPNTASAPTESLTWGQAIEWIPLLL
                     GNVESCNRDKAALRRIDKEKANETTQAQ"
     gene            10188..10496
                     /locus_tag="ACHUT9_001280"
     CDS             10188..10496
                     /locus_tag="ACHUT9_001280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347801.1"
                     /translation="MKQRKRNKVANKRVVTPARIEHERYTHLNARLDRIDARLSRLAV
                     PHDISEQLSQLDVQLSELYSVTKESAIKYGAVAGAMSGGVAGLMVSTAFLLLRSKLGM
                     "
     gene            10496..10996
                     /locus_tag="ACHUT9_001281"
     CDS             10496..10996
                     /locus_tag="ACHUT9_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFR1347802.1"
                     /translation="MAYPQDTRDRLRRAYIYNQWSLEVTASQVGVSFPTARRWKKEAQ
                     DAGDDWDKMRTANLIAGGGMEDAGRAVLMSLVTQCQAATEAINGAVDMPADKRVELLA
                     SLADAFNKATSASKKILPETNRLAIAIEVLQALGEHIERKHPNHKAAFVDILGSFAEV
                     MESDFG"
     gene            10983..11198
                     /locus_tag="ACHUT9_001282"
     CDS             10983..11198
                     /locus_tag="ACHUT9_001282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347803.1"
                     /translation="MISDKEELESWLYLLRSTVADGTPEGQAAVQASTEATRAFIEKL
                     KTDYPDKSGLFGGLIAALECAVQEMSD"
     gene            11209..11436
                     /locus_tag="ACHUT9_001283"
     CDS             11209..11436
                     /locus_tag="ACHUT9_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016150493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347804.1"
                     /translation="MERVVLTVDQIKHLASFAGLTVSAPKALNVEAQYIICHGEVPAF
                     ESDEGEKIPAYSGLIAYTDDVSDSGVLELDI"
     gene            complement(11470..11970)
                     /locus_tag="ACHUT9_001284"
     CDS             complement(11470..11970)
                     /locus_tag="ACHUT9_001284"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347805.1"
                     /translation="MMSFDIKGTIAGIKASIDALKNALELKQSIATNKEVGEVYAQLN
                     LLQDQLITQSVRYDALAREKEAIEEELIQLKRHLDDAEPYELFKLPAGSIVFRTKPDE
                     NAIDPTHYWCADCYHKQIKSVLQPWTPQNSHTTLHCNTCGATLLNERIPMTMTVAYNP
                     NKRGAF"
     gene            12159..13814
                     /gene="terL"
                     /locus_tag="ACHUT9_001285"
     CDS             12159..13814
                     /gene="terL"
                     /locus_tag="ACHUT9_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005276842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFR1347806.1"
                     /translation="MKSNRSKFSKKALMAELQEYVANLRQTIEAECLGFDIDPVASAE
                     RRRQVADAVSGYDFFVSTYFPHYIRNTARSQLHNYLFQRLPQMVASTVGENDAIAAPR
                     GEAKSTLVTQLFTLWCVIRGIKHYPVIIMDSIDQAYPMLEAIKAELTFNPRLKHDFPE
                     VCGQGRVWQMGTIVTANNIKITVAGSGKKLRGLRHGPYRPDLIVLDDIENDENVRSPE
                     QRDKLEAWLKKTVLPLGEAGGKTDVLYIGTILHYDSVLSRTLNNPMWRTARFKAILQW
                     PVNMSLWDRWEELLRNQQVEAADQFYREHEAEMREGAVVSWAARPLLALMKIRVRDGH
                     STFDSEYQNDPVSGDDAIFAAPGVLKFWKEEVREWVFYGACDPSLGKFGNNRRDPSAI
                     LVGGFNRLTGVLDVVVADIRRRVPDKIISDVIEYQRRFGCLAWSFESVQFQEFLRTEL
                     VKRSAQLGVPVPAIPVIPSSDKLLRIESLQPHIANGLIRFNQETQSTLLDQLRHFPMA
                     DHDDGPDALHMLWSLAVSRAHSTQIFTRSRHTYSGARLGSGAW"
     gene            13822..15402
                     /locus_tag="ACHUT9_001286"
     CDS             13822..15402
                     /locus_tag="ACHUT9_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFR1347807.1"
                     /translation="MPQIVDQFGNPLKKEALKTPQTIKMAGTYHQYPEHLSKGMTISK
                     LPRLLRQAELGMLSAQASFFEDMLERDGHVFAEMEKRKNALLTLERTIVPPENASSVE
                     KNATAAVKEWFSGIMELEDVILNGATAIGHGFSCQEIQWVTVENVLIPQHLHLRPHYW
                     FNSLPEKGDELRLNSTNPDGDVLWPFGWLVHRANARSGFVGSSGLFRVLVWPYLFKNF
                     AVRDLAEFLEIYGLPARIAYYAPGTSDEDRDQLLMNLIRLGHDAVAAVPTGNEIKFES
                     AATGGSDAFMALIEWAERTESKAILGGTLTTQADGKTSTNALGTVHNEVRHDLQAADA
                     RQMEGMFRSLIQMMLALNGFGDIPIHRQPRLVFDTRKKADLSQFASAVSTLVNAGLDT
                     IPVSWIHRESGIPLPQDGEAVLTPRQTGLANLRQAYPVSPGVGVAALSQTEDTPESDP
                     GQQAIDTMPPLSDAVGDAMNQLLAPMIAALQQGQTADEAMNILAEGYPQLDDTQLRAL
                     LTQAIFVTDLWGQLHANS"
     gene            15389..16555
                     /locus_tag="ACHUT9_001287"
     CDS             15389..16555
                     /locus_tag="ACHUT9_001287"
                     /inference="COORDINATES: protein motif:HMM:NF016148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1347808.1"
                     /translation="MPTAEKVDLAYAIGLSPAEAVAYFESKGYVIGLHWYDVYAEAHA
                     KGFTVAGVLKLDILKDLREGLQKALQEGSTQVDVERQLLPILERKGWLGKGLVVDQST
                     GELLGKRLMPRRLDTIFRTNMQSAYNAGRYREQMANAASRPYLERVAVMDSHTRPAHS
                     RLNGFIAPIDSPVWGFLYPPDAYGCRCRVRARSAAEVEKLGLTVHNPDVIEIEQECGI
                     PGEVRKVPAMINPFKGTVYAADPGFGINPGKVSWQPELDKYATPAARQYITATLTGPD
                     FARSYRKAIAGTVDKQQRYPVAMLANPIDGSRVVSVDGATLQNMATGTPTVTSEMFLL
                     AQQAIQHPAKTVEQQGATWYCVMRQGEWWVAHVAHGQLIGLTHGTDLDALLPEV"
     gene            16556..17104
                     /locus_tag="ACHUT9_001288"
     CDS             16556..17104
                     /locus_tag="ACHUT9_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1347809.1"
                     /translation="MLTVDIDTRGVQRHLNHLLQGIKHRQPLMQALAGDMWNAVEENF
                     KQQGRPTWAGWSPAYAARRGPGQILQRKGRLAASIVPVADNQMARVGTNVKYAAIHQF
                     GGTINMPARSQRAYYRQRKDGTIGNRFVHKSRSNYSEWHTLPAYNIEIPARPFLQLTD
                     EDISTLEETAQHYLQGVIDGNR"
     gene            17313..18458
                     /locus_tag="ACHUT9_001289"
     CDS             17313..18458
                     /locus_tag="ACHUT9_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFR1347810.1"
                     /translation="MKTRLAALSVEIAAGAEPVSRLRILPAGEFRGVDGRPRECAAWV
                     MTATAAARIIETVNSRVTELCFDYEHQTLRAVENGKPAPAAGWFSQLEWIEGEGLYAT
                     DIRWTEIATDMIRKKEYRYISPVFFYDQVTGEVTALVNVALTNTPALDDLDEVAVAAL
                     SRLAALSTTSSQSEGNTMDEEQIANLLADLRWILNLPKTATAEDIKVELDKVIAAMSD
                     NQGVAAASQGLMPWIAQQLSVKDQVIATCNTQIASLSAAAYDPTKFVPIEAVTQLRAQ
                     LSQIQTQAATQELDGLVQAALSDGRLVPAMADWAKELGRKDMAALKTYLDGAPRLAAL
                     SAMQSNKTELNAPQHRQDDSALDAAQLAICSSLGIEASDIAKELGEI"
     gene            18458..18853
                     /locus_tag="ACHUT9_001290"
     CDS             18458..18853
                     /locus_tag="ACHUT9_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347811.1"
                     /translation="MDRNTPFNDGKLFPVPCEAGAKIDVGNLVCVNSAGYAIAGAKGS
                     GNTLLGLCDEAVDNTNGAQGERVVMVRRGLAVLLENHSGDAVTQAEVGKPCYLHDSVT
                     VSKTDGGNAPLVGRVLTVSAHSGVQVLID"
     gene            18863..19768
                     /locus_tag="ACHUT9_001291"
     CDS             18863..19768
                     /locus_tag="ACHUT9_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFR1347812.1"
                     /translation="MLINKTNVTTFFVGLKKLFNDALKREPGQWEKIAMKVPSGTKTE
                     DYSWLSDFPRMRKWIGEKFVKSLSAFKYSITNDDWETTIEVDRNDLEDDQIGQYALKA
                     KSAGRAAADLPDDIVFALVNDGFKTTCFDGQYFFDTDHPQAGGTVSNKGTKALSCASL
                     AEAKASFGAAKMQMRRFTDSEGRPLSIRPTVLLVPPELEDTANTLMTADRFEDGKVNI
                     YKGSCEVVVEPRLTSPTAWFLLDTSNDVKPFIYQERKAPKLVEQTDEVADDVFMRRKF
                     KFGAEARAAGGYGFWQMAYGSTGEA"
     gene            19771..20097
                     /locus_tag="ACHUT9_001292"
     CDS             19771..20097
                     /locus_tag="ACHUT9_001292"
                     /inference="COORDINATES: protein motif:HMM:NF036841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFR1347813.1"
                     /translation="MIYITAKRNGFYRCGIAHSDQTTAYPDDHFTPEQLAALEAEPML
                     IVTRDNPQTATQEAAEQQIAALKQALTQTQQHNAELREELDAAHARLAELTGDAKVTT
                     PKAKAK"
     gene            20109..20537
                     /locus_tag="ACHUT9_001293"
     CDS             20109..20537
                     /locus_tag="ACHUT9_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFR1347814.1"
                     /translation="MYATLEDMLLSFGERECSSLSDPDYSGGINEAVMAAALQRASAE
                     IDGYLVARYPTPWPDTPRILVGRCCDIARYHLATASRQCTNEIRMRYEDAIRFLEKVA
                     SGVISLGRTDSGDPVKAGSQMRFMSTPRQFGRDATGGGAF"
     gene            20534..21193
                     /locus_tag="ACHUT9_001294"
     CDS             20534..21193
                     /locus_tag="ACHUT9_001294"
                     /inference="COORDINATES: protein motif:HMM:NF020447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFR1347815.1"
                     /translation="MITEIENAVIDRLKAGMGRMVRDVVSYGGELDDIGEIVRALPAA
                     WVTFGGITHTKAVSTSNKRFRTHGRFVVMVGDYNARCEVAGRQGGVRIDEVGCYRMIY
                     AVRRLLMMQSLGLDITPFQPGKVRTLFNTRMNEKALSVFAIEFDTSWIEAPLALGAWP
                     MREDCATEPDHIFTRYRGKLAEPDAPISRIGLSYDPPGIGSREHPADLINLDEDISDE
                     S"
     gene            21183..21383
                     /locus_tag="ACHUT9_001295"
     CDS             21183..21383
                     /locus_tag="ACHUT9_001295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR1347816.1"
                     /translation="MKVKAAKGLLVPKENNPFRYIDDRAFVEVPDSAYYRRLLQSGDL
                     QGKPVTGKTATVAEAHTEDKTE"
     gene            21380..22801
                     /locus_tag="ACHUT9_001296"
     CDS             21380..22801
                     /locus_tag="ACHUT9_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HFR1347817.1"
                     /translation="MSITFDTIPGSTRRPGQYLEFNTRMAVRSLPGNLQTMLIIAPLM
                     KSGTAKPLELHNVFSDDEAATLFGAGSLAHLMSRAAILSYKYLQLQVIGIDEHSAGRA
                     ATGTITLTGPATGNGSLSLWISNMRIDVAIDDQDDADAMHNALAAAINADSLLPVTAA
                     VTDSDGKKVTLTAKQKGAFGNDIMLLGSCNAAGVTLSVQAMSGGQHNPDIQPALDAVF
                     AAGHNVIVAPYSDQTTLTALREHLKKTGHAMEQRGAVGVAGWPGTLSNGTTLAGQIND
                     GRVSLAWHSGSRCLAGIIGAAYGAMVASEEDPARPLNSLPLAALDITPLTARSGGTEQ
                     ENALHNGVTPLEIGPGNVVQIVRAVTTYTRNPQGVADPALLDLTTIRTLDYTRKACRE
                     RIALRFPREKLHERTRAKVKSELLDVLLKLEEEEILENVLANKEKLLVERNAKDPNRL
                     DAVIPADVVNGLHVFAGRVDLYL"
     gene            22813..23184
                     /locus_tag="ACHUT9_001297"
     CDS             22813..23184
                     /locus_tag="ACHUT9_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016192711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1347818.1"
                     /translation="MTQEYMGAIVLEVDGQEIEVTSFDVKTTTGRKLVKTMNKTGRAK
                     GYCQGIATYDLSLSVVIPDTGDLDWENIVDAKVTLYPLNNQDKRTSYLGCFSIDISEK
                     YQVDNEAQRDLTMAALRKVDE"
     gene            23181..23558
                     /locus_tag="ACHUT9_001298"
     CDS             23181..23558
                     /locus_tag="ACHUT9_001298"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347819.1"
                     /translation="MSMTVKGSLRYGIEHNGKLHYDFEIGLLTMGQSGECLDATESRF
                     GSLDSRQADAFYRAASFAYALRQLGDMTKADITPELLYTQLTQDDADVLDKAVDEVKK
                     KRSEPKSAKLACDSPSSSSVNMA"
     gene            23719..25923
                     /locus_tag="ACHUT9_001299"
     CDS             23719..25923
                     /locus_tag="ACHUT9_001299"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1347820.1"
                     /translation="MARDFKVSMTLEAKDDASRQVARALKETTGHAEKAAKAIKQQGK
                     AQQQAAASAAAAAKQQARDSIAASRAQLDEARRMQRAREALGIRSEQAIQREIDRTIA
                     SYNRLTRAGTLSAREQARAYRQMQHQVAELRREMKGVTGLQRASAFFSKSGAIVGGAA
                     AAGAAFVSPIRNRMNYDERLTEMANTAFSDADLKGRQAGLQRLDKMIRDNAMSSGLNK
                     ESVMDTVNTLLASGLKEDAVSKLLPTITRYASAAKADPNELADIAVKARSTFGISDDQ
                     MPTMLNMAIAAGQSGNFELKDMAKWLGQQMAFAKKAGMSGLEDFGKLLTLNELAAVTA
                     GSSDQAGNNVVNFLGKLTSQDAANAAANVKVSGNKGIDLPGTLAEARKNGIDQIEAFS
                     RMIDEVVKSDKKYQQLQAKLKTLPKDDKSGIKETLEAMSAILEGAGVGKILNDQQALM
                     AMIAYRNGQGYKNEIEKNINKQRNLKPGEQGAGDVNFQFNKEQGWFKAQQFKNAKEFA
                     ELDSIKSLSDATGDAAGKVAEWAEQYPTLTAAVSGATTAIQGMTQAAIAFAGIKILTG
                     GSAGAAAGAAAAGSAAGTAAKAGSAFGWISKALGPAMAVTAAATFTSAEEEDELLNGE
                     AKWKALREKYPQEVIDKARKKYQPWYQFGRGYATENERWIKQYLETEGLGLPTPQHVM
                     NAIQPVTPQSTTATTPPPQPTAPSSQVIRLEVDGQVLAETVNRFNAQEGVRG"
     gene            25920..27362
                     /locus_tag="ACHUT9_001300"
     CDS             25920..27362
                     /locus_tag="ACHUT9_001300"
                     /inference="COORDINATES: protein motif:HMM:NF018818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR1347821.1"
                     /translation="MSWRNNLQDASFKGLRFDVIKETTAHSRDYAAHEYPFVNGADIH
                     DLGRKARNLRLTAVFWGEDYDYRLQNFLAVLDSAGAGELIHPIYGSIPHAQPIECQVS
                     HEADTPDYCTVELVFLESGAGKNLQPVVRPEQWGDTLFNSLEALQGDVMALYEAGMAP
                     ITRTRRLLTKGKTALSAMLNTLTIMRSGVKGVFTDTADYLAFPKRYVNDLRAVLDLRT
                     LATDTVLNRQHAQRTGSTTGRIYGDTHVVFTPQGANANDDVMQNLVAYKAGLNTVQTA
                     LKDPAPLLSAWKRDCGILEQVMQFPVALLNRSTTAVVPIPDTAKMDDVCDVVTLHTSL
                     AAMRAVEIFTDVLTDDAALVLTPTHIEQMANQARQYIQNALELNRETYKPFTTAISED
                     PDARGLLWPPVNERLKSIALGVQVLAEQQITRRPPLVVRVVTHDTNLHLLAHAWYGDY
                     QRAPELLRLNPQLVDPNAIKAGDKLNAYSR"
     gene            27346..28530
                     /locus_tag="ACHUT9_001301"
     CDS             27346..28530
                     /locus_tag="ACHUT9_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR1347822.1"
                     /translation="MLTVDNDNITLLLAGKAYSHWQRYRIDSDFLKPADGWQLGLGLP
                     SGTFPADAVRGAPVKVMAGDDVILSGRIDSVQREVSRRGLVLSLSGRDNAAVLVDCAA
                     PIYNARQLNLDEAISAIVSPLGIKRVRIQASSVTRNDMIHIEPGERAWDALVRVASAR
                     GLWPWCDPDGTLVIGGPDYTKAPVATLQLSRDENKTNVLSLVDNRNISGCYSELTMLA
                     QSHATLADSQPVVIDVTAPHSDTESADTLSSGETGRYNRSHTEKDPTVQWYRPQILVV
                     GDVDNAEQLAYRARKAMADARLSGLDITIEVAGHRTASGELWTPGQRIHVISELHGID
                     DVFFLMGRSLNGGRPSGKTTILRLKEDGVWLPDAYPTDKKASRRKKKKPELVVLPVWE
                     KN"
     gene            28533..29189
                     /locus_tag="ACHUT9_001302"
     CDS             28533..29189
                     /locus_tag="ACHUT9_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1347823.1"
                     /translation="MWNEVNRRISQALGRIRLAFRIAIGSVDSGAKVQTIQAAGIGGE
                     SLRGNELFQQYGFTSCPHPGTMGIVLPLGGVSTHGIVIATEHGAYRLKGLKAGEVALY
                     DDQGTKIVLKRGRIVDIECDAFRVNCKNYEVNAENRADFNTPSLNASQALTAEGKISG
                     NGGIGIKGGKGAEFEGSISQRGGSFSTDGDVRTSLVSVNHHAHTNGHDGRPTGDPIPQ
                     "
     gene            29247..29600
                     /locus_tag="ACHUT9_001303"
     CDS             29247..29600
                     /locus_tag="ACHUT9_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR1347824.1"
                     /translation="MDRLISPKTGDYTGTRCRSLENAVYLRLKTPLGSYWADKTLGSR
                     LHELAREKDLTRVHLLARQYAEQALTPLIRDGRASHIAATTQKSRAGWLTLQITVVDS
                     GGDEYCFEHPVRVMA"
     gene            29600..30667
                     /locus_tag="ACHUT9_001304"
     CDS             29600..30667
                     /locus_tag="ACHUT9_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1347825.1"
                     /translation="MAFVYEDMATIRERQLRDIRNQLPDEDIDTGSDSDHFVRASAVA
                     SVAEGLYQHQAWIVRQIFPDTADPDYLRLHARLRGLEKKEATYAEGIASVTGTLGATL
                     KVGAVIRNATRTCRTIEPLTLAEGVNTVKVRADTAGVAGNLAVAESAELVSAPMGINS
                     RVQIMQLSGGTDDESDASLLARLLDIIRRPPAGGNQYDYRRWALEVPGVTGAYVYPLR
                     RGLGTVDIAITSAGALPSESVIKATQAHIDDVRPVTARDALVLAPTQKRVDFDIAVSV
                     DGITLESAKTTISETIKDAMSRINPGQPLIRSQIEMLISLIPGITDRRIITPADNVIA
                     RVDKSHLEWLVCGSISVRSGV"
     gene            30664..31230
                     /locus_tag="ACHUT9_001305"
     CDS             30664..31230
                     /locus_tag="ACHUT9_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003030319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFR1347826.1"
                     /translation="MSHKQLLMALMPPVSYTPTEKNLRAELTAEGNVFDAVKQRAESV
                     LGAVTPFFAQELLPDWERVLDVQPQAGDTYQQRLSRVLAKISETGGLSIPYFIRLAAS
                     MGYQITIDELDAFRAGRNRAGDVVYAPEVIWIWRVNVFNSRVQTYRFRAGGSVAGERL
                     SYFADSIIELIFNDLKPAHTFCYFTYQG"
     gene            31234..32637
                     /locus_tag="ACHUT9_001306"
     CDS             31234..32637
                     /locus_tag="ACHUT9_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011147678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1347827.1"
                     /translation="MQDLMPTINTPDNVFHDGNDATGELGTVVSALWLNNIQSAVRDI
                     QAECHAILTANGFTPDSSRQNQLLQAIQKAITSKVPSASTSQSGLVQLSSAVNSDSET
                     TAGTSKAIKTAMDNANARLAKSRNLSDLDNPAQACQNLGLSQAIKNADGAMQKNANGA
                     DINDKQIFIDNLGLRETVNKASNAYPGVKLLDAPPGKEYIGAFSCSSQGQHVKGMNIG
                     VQSSDCAQVLVDANAELRIKFFNGNGAVKENAIFNGTRAFNTLPERDYAGAFTCGSND
                     SYAKGISIGVGADSDVGQIWLDSSANLHTRFLNSNGDIRRNKIVGVPIGATIEWQSTA
                     PIPAGFLENNGRSFSAPAYPELAKVFPNLKLPDDRGLFKRGLDNGRGIDSGRSIGSEQ
                     GDAMRNISGSVGGITAESSGVAVSGPFSYSYSSGGRAAGSGAGMLGFTFDASRVVPVA
                     SEFRPKNRAVIYITRVF"
     gene            32639..33262
                     /locus_tag="ACHUT9_001307"
     CDS             32639..33262
                     /locus_tag="ACHUT9_001307"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1347828.1"
                     /translation="MNKYSVDIMDAVFDDAGIAVKSGWVKIYHCHPVTREFIGAGMDF
                     VHQGFSIPADAYLDAPHLPEQTKNIAICRNVDGNAWVALVDYRGCTAYKTATGEPIVV
                     DFIGDLPETLTLIAPSSPFDHWLDGQWVTDTSAQHAAAVSEAETKKTSLLAEASTIIA
                     PLSDALAGGYITDEDSKKLLAWQKYRYSLTKVDTSLAEQTAFPMKPS"
     gene            complement(33709..34128)
                     /locus_tag="ACHUT9_001308"
     CDS             complement(33709..34128)
                     /locus_tag="ACHUT9_001308"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347829.1"
                     /translation="MAKIQVRNFPDDLYRRLEEMAAMSERSIEGQARLLLKEALEDKS
                     TANNPNNRPPALYKQIIPSPDWYFRHDNVEGSPTKSTVYQLAAWALTHEGEIVGLVTV
                     HDPKTGRPILVTPPPVAGDYLHKEQLENDEVLAAKCR"
BASE COUNT         8927 a         7712 c         9119 g         8558 t
ORIGIN      
        1 ctgtccagtg gcataaacag tacccgatgt cccgcatcca ccgtgtgggg aggtgatcat
       61 ttctggacta caggggaaag cggggaaaaa ccttgaaagc caagcgggac aagggattga
      121 ggcttagaga gcgattctgg atcgttttgg tgaaaatgag caaaaagcta tttttgtgcg
      181 cacaattctg tactgttttg atcaaagtct gagtatgttt aagcggcttt taaatccgtt
      241 tgaacatgtc tgtaaatcca tattgtcagc tatctatatg ttgccctcag tagtggtgtt
      301 gagtggcgct cataatcctc gtaataattg ccccttcgca ttggaggggt gcatagtctt
      361 gttttcattg tgattttaaa tagcgcccca tttctgaggc gcatcatgct gttttcttta
      421 ctgagtcatc atccggggta gtaggaatct gactaccgtc cggcgaccgt tcccgtgcac
      481 gagattcgat gcctcgcaaa atctctctgt cagtgtattc atctccagcc agagccatgc
      541 agaatgcgtc tttgagggat tctctgatgt taagtgctct gatggcttcc tctacgctat
      601 ttggttgaga ttgaatatgt tgagtcctat ctaaaatgcc tttaatgcca atttcacagg
      661 ctagagtagc taaagcttgc cgttcatcac tcccaacaaa actaagaaga gagaccagtg
      721 ttgctgttgg atctgctaaa ggctcgtcct ttgggtttga gataggctgc ttataagtgt
      781 gagaatcgga gtcatcttct cctgttaata gccaatttaa cgacacccca catgcgtcag
      841 ccaccttggc tgcactatca attgtaggat aactcccacc tttcaaatac ttgcgaatag
      901 atgcttctga caatccacta cgtcgagcta attcactttt actgatttgc cccatagctt
      961 tttctagtcg ctcgctaaac cgtactattt gattgtaatg aaaagtacgg tcatcactgg
     1021 ttttactcat actgtactat tccaataact aattgatttt tataagaaac aacaacaaat
     1081 cgtactttgt tgcgatcttt gataaaagta cgatattggt gttgacatcg tacttttatt
     1141 cgatctatat ttatcgcatt cggtaatcgc aattgattat cggattcgat aaacacaaga
     1201 ggatcacata atgcagttgt cgaaacaaga cctgactgac ctgtttattc gtcccggtca
     1261 ggattggtct gcaaaagcaa tttatgcggc tcttgataac aagggtgcgg atatggcagc
     1321 gcttgagcgt gctcaagact tgaagcccgg ttcaatgcga aatgtgtttt atcgtccgtg
     1381 ccctggatat gagcgagtta ttgcgggagc cattggtgtt gagccatatg tgatttggcc
     1441 cagccgatac caaaacaaaa gtacgaggga tgtggcagca taaaggtgat ggtatgtggg
     1501 taacagctca gcagtgcctt ggcctagccg gttttcccgc ggcggaaaaa aatgttcggg
     1561 caaggttaga taaacttacc gagcaaaacc ccgagtggaa acgcaagcgc aaagggacta
     1621 aagcatttga gtattacatt gaatgcttac ccgctgaggc tcagcggatt ctcaagaaaa
     1681 aatatactca acagcttatg aatgatgcct ccgttcctgc tgttgttgta gcaaaaccaa
     1741 gtaaacaaat tgctatgcgc caagagttgg cggtcatgaa caagtgctcg gagattgctt
     1801 tacaagctgt gcagtctttg acggacaagc aaaaagcgat tgctgatgca cgtatcttac
     1861 tggccgcaga ggtgcataag ttgcgcgaat atgcaggtat gacgcgcacc gcagcagtgc
     1921 aacatattgt tgatggtgtt cgctgtcagg cattacctga gcgcattatc actgcggcca
     1981 ataccgcaaa tgcacgacaa ggaacccgca caggagtaag caaagggagt ttacaagagt
     2041 ggtattccac ttatttgctg gcgcaaggaa atgctattca gctgcaagcg ttattggccc
     2101 caggtcatca caaagaagta ccttgggagc aagtcgcttg gttgaatgag ttttttatgt
     2161 tttatcgagt gtggactcgt cccaccctcc agcaagccta ccggcaattt gctgagtggt
     2221 ggaacgatac ttacgctcac aatccagatt tactggccgc attaccctct gttcatgctg
     2281 tgcggcgagt cttggggaaa gtgcccaaaa tagtaaaaga gcgattccgc tacacgggat
     2341 catcatggcg ctcgctcaat ccgtttgttc ggcgtgattg gtcaacccta ccggtaaatg
     2401 gtgtgtgggt gggtgacggt cactgcatga aattaacggc atttagccct gatggcaata
     2461 tagctcgccc agaagcgact tttatcatgg atgctgggca gcggtttatt gttggttggt
     2521 cattatcgtt atcagaaaac gtaattgcag ttgcggatgc actgcggcac ggaatgagcc
     2581 agcacggagt cccattgatt tattactccg ataacggcgg tggcgagaag aaccgacaat
     2641 tggatgctga tattacgggt atcttgcctc gattgggggt tgagcatcac acaggtattc
     2701 ccggcaaccc acaggggcgc gggattatcg aacggtttaa caagatagta cctaaagatg
     2761 tagctatcag ctttcagagc tattgtgcga aagaagctga tggggagtcg gtacgattac
     2821 agcaacggat agcgcaatcg gcattgaaag cgacccacaa aggaaaggat ttaaccaaac
     2881 gccaagtcga ggcgatgggg aaaatcccga cttgggagca gcttttaagt gccattgaac
     2941 aagaggttca caggtacaac aaccgtcccc ataccagcct gcctcggcgt gataatgggg
     3001 agcactatag ccctgcggca tatcgcaaaa agttacttgc ggagcaagga gctgaaattg
     3061 attacctgtc accggaagag ctgcatgaaa tgtatcgacc ggaaattatt cgtaaaacag
     3121 aacgcggaga agtcaggtta ttcaataaca tttatttctc gcatgcgttg gcgacagagg
     3181 ccggtaatga agtgcgggta tgttatgaca ttcatgatgc tagctcagtg attgtgcgtc
     3241 gcatggatgg tgcgtttatt tgtgacgcca tttggaacgg taacaaggtc gatgcctttg
     3301 ctaaacctgt tattgagcga gttaaacaac agcgtgttaa aggccgtatt gctcgcgcga
     3361 cagcggttat cgaagacgct aagcgtgaac tgcgcccagc attggaacaa agcgtccctg
     3421 agtttgatct tgggtatatg cagcagcatt taccgcatga acgagaagcc gaagagttat
     3481 atctgtttgc agcggatcgt gatgcaagtt taaagaaaag cggaacgcat aataaataac
     3541 tgaggttttt attatggatt tagtcgaaca attaaaagat gttatggagc gccgaggtta
     3601 ctcccaaaca caagttgggc gtgctattgg tcgcagtggt gcggtaatca accaattttt
     3661 gcaaggcaaa tacacgggcg ataatgctga tttagagtta cgcattgaag gatttatcgc
     3721 gcgcgaatta gagaaacaaa aagcaaaacg aattaccgca cgttttattg agacaagcac
     3781 tgcacgtcgt ggcttagagg ttctgtcata cgcccaccaa gaaagtgaaa tgtgcgtgat
     3841 atacggtgcg gcgggtgttg gtaagacgat gattttgcgt gaatacgcag cacgtaatcg
     3901 agatgtatta cttattgaag ctgatcccgg ttataccgcg cgaacattgc tgggagagtt
     3961 gtgtcgccag cttgggttga aggttcgcgg taacattcac gagctgattg atgcctgtgt
     4021 tcgtgagctt cgcgggtctg cgcgcttggt gatggttgat gaagcggagt tgctacccta
     4081 ccgcgcttta gaggtattgc gtcgtctgca tgataaatct ggtgttgggg ttgtccttgc
     4141 tggtatgccg cgcttattaa ttaacttaaa agggcgcagt ggtgaatttg cacagttata
     4201 tagccgtgtc gctctagggt taaacctcga tgatagtttt tcgcgtgatg attttaacgc
     4261 tctagcctac gggatgatgc cagaggctga tgatatagag attagtaatg cgctttatga
     4321 gcgctcaaaa ggcaatcctc gtcgtttatt taaattggct cgcggtgtct atcgtatttg
     4381 cgacattagc cgcgctagcg tcagtgttaa agcgattgat aaatttgctg agatgttaat
     4441 tcattaatag tgaggtatgg atgtcagctg ttgttacttc tttaagtgca gaaaagcgtg
     4501 tattgcgctc tttttccagc aatgttgttt tcgagcgttt tccgtcaatt aaacattatg
     4561 tttctctggg ctcagctcgt ggaacggtgt acttaaacaa gtacgattta atgcgcgacc
     4621 ttgcaggagt tgatgatgca atatcgtgga cgattgaaga gcacgatatg ttgatgaccg
     4681 agtatcgctt tactaatcga gatgaattta ccttgatttg gcgtcttgct cggcaagagc
     4741 tgcatcgcac catgaaaatt gcgcgtttaa tggggcggca ctcgttaatt agcaaataat
     4801 tgcatgatgg agaacaagca tatggagaag caaactatca atgccctgcg ctctcgtgcg
     4861 cgtgttgcga ttcaatccca aggcggaatg gtaacaaatt ggagtcagcg gttccccctt
     4921 ttgtttgctg tgcctccttc tggtggaata gcttggccgg ttagccagct agtcgaaaca
     4981 gtaaacggga aacggcgggt aataaaaaca tcacatatta atggatgcac cgtcatctgg
     5041 cagtaaacca ctaaaaatcg attatcaaaa taaaacgtca ataccaccag tgcatctggt
     5101 gtggggattg tttgtctaaa aaccaataag aggtaagcgt tatgagtatt gaagtaaaaa
     5161 tttccattaa agaggatgat ggagggttgg gtgtgtcagt aagggccaag catgacggtg
     5221 aaataactaa ggaggaggca aagttttcac ttattttaag taaagtcatt aacgcgtcgg
     5281 tactaaatat atacaagggg cacgttgtta cgaaagaaca aaacggccta tttaatacaa
     5341 gcgaagaaca gaaagaaatt attgaaaaga tgcttagggg tactcttgag caacaagacg
     5401 aaaaatcaaa accaacagtt cactaatcta ttaaggggga atatatggca atgaaaacca
     5461 aacgaatcaa aagcgcagca tctctgtatg ttccacaaag caaagaggat gtcattagtg
     5521 acatcaaaaa aattggcgac ctccagcgcg aacttgagcg cacggaaacc atgatgaatg
     5581 atgcaattgg tgacattaca gaacaacacg cacccatgat tgaagcactg aaaaaagaca
     5641 tcgacgtgct gagtaagggg gtgcaaagct ggtgtgaagc gcaccgtgat gaattgaccc
     5701 agaacggcaa gaccaaaaca gccactctaa ttacgggcaa ggtcgaatgg cgtaatcgtc
     5761 cgccatcagt tgcagtacgc ggtgttgatg ctgtcttgga aacgctgcgc cgcttagggc
     5821 ttgagcgctt catccggaca aaagaggaaa tcaacaaaga tgccatcctt aatgaaccta
     5881 aagccgttga gggggtggcg ggaatttcag tccgcagtgg tattgaagac ttctccatta
     5941 cgccatttga gcagaatacg ggggtgtaat atggatggat atcgaatcaa gaatacattt
     6001 atggcttatg ccgtaggtgc tgcacgagct gagcgcttta aaaactggga tattgcggca
     6061 gacttgtggg aaaaggcggc attgcgacca gttaatgctc accagcacga atgggccacg
     6121 cggcgaaagg cgttttgtga aaacgcgtac cttaaaggct ggtaacagcg cttaattaac
     6181 gccttttaaa acctaattta aatcactctt taaatatggc gtaaacccgt cagggctggt
     6241 ttacgcctaa aaaagggatt tttcacaatg agcaaagaaa aacagataga caaaataaaa
     6301 aagctattaa acaaagcgcg caataactca agcgcaaagg aggcagccgc agccttgcga
     6361 atggctcgca agatgatgca ggaaatcggg ttgcaagaga atgaaattga gttgcttgat
     6421 atcacattaa gtgacagtca aagctcgccc agcaatgcac ataaagcccc tcactatgtc
     6481 gcaatattaa tgagtgcggt aagtcgggct tttggtgtca aagcgttaac cgtatggaag
     6541 cgtttcgatg atggctctag taagcgctca gtgcaatttt atggcccacg agagcggcca
     6601 gaaatcgccg cttacgcctt tgatgtgctg gtacggcaaa tcactaaagc acgtaaggag
     6661 tttcttgcgg ggctgcacaa aaacattaaa acggccaata aaacagcgcg agcagatgcg
     6721 ttctgtgagt attgggtgat gggggtttat agcaatttag aagaatttgc ctgcacccca
     6781 aaagaggagg cgcttattgc tgaatatgag caacgtcatt ttgaacatgc aaaagcgttt
     6841 tctgggcgta aagccaaaga ggttcgaggc gttgatgaag ctcgatgtaa agggtttggg
     6901 gcgggtcggc gcgtcaaact caatcatgcc gtaacgggta aagcctccac ctcacaccaa
     6961 ctggaggcca gataaaatga gcgttgttta tattgctggc cccatgtcag gtatcgcgaa
     7021 ttttaaccgt gccgcatttg atattgccgc gcaggcgttg caacgtgagg gacactcaac
     7081 actgaacccc gcaacattgc cggatggatt aagtcagcgc caatatatgc agatttgctt
     7141 aggaatgctg ttttgtgcag acgccattta tctcttagat ggttggattc actccgcagg
     7201 tgcacgcgcg gagttcgcac tagcagaaaa gctcggattg gatatccatt atcaaactca
     7261 ggatatgggg gtgatacatg gctaagtcac ccgcagagcg taagcgtgaa caacgcgaac
     7321 gcgaacgagc cgcagcacga cacgctgaga tctccggtca tcgtcgtatt gtgttgacag
     7381 tgagtgagca cacctttcag caaattgaga tggcgcgtca tgcacgcaga ccgggtgcag
     7441 agccttatgg gattgatgaa tattttgagc tacttgctgt tgaggatacg cggcgcttac
     7501 agaagcaaat ggccgaattg tcccagcgtc aatgctcact ctgtggcgac acattgccgg
     7561 gtgatgctca ggggtgtatt cgacgaggag agcatggctg tggtcaagat gccgtgcgtc
     7621 gattgttgat gctaaaaaca atgtgatggt agcgtgacat gtcacattat ttttcgtgac
     7681 atgtcacgat gttccaaagg ttgtggagct aagtgatgat gaagcgtttg attggtgccg
     7741 tcaaggctgg gcaagcattt ctggggtggg atgactcgat ttatcgtgct gttatttctc
     7801 gcttaactgg cggaaagacc agcgccaaag cctgttcggt acaagagcta tcgaccataa
     7861 aagaatatat gcatgagcaa ggtttcccgc gtaagcctcc taaaaacaaa gggcgtcgac
     7921 caaatgttgc acgagacaga aaagcgattc tgtccaagat agaggccctg ctggctgatg
     7981 cggggcgctc gtggaaatat gccgaggggc ttgctgcgca tatgtatggt caacatgttt
     8041 tagagtggct aacctatgaa cagttgttcg gcgtgatggt ggcattagcc aaagacgctc
     8101 ggcgtcgaaa tgcgccatga tatcttgata accccgcaat catgcggggt tatcttttat
     8161 tgtgcttcat gatggtgaga gggggtaagt taaacttacc ccctttacct atctgtacag
     8221 gtgatcagtg ctgggtataa ttacatcacc tcttgctgtc cgagatgacc acgatgatac
     8281 tttgtgaaac tgacttggca cacctacaag actccctgcc tgaatctgtt catacattga
     8341 ttgctatctt gggttactcc gccactatgc gacttattag cgcattaggt ggctcaacat
     8401 tgcacgcaag aacgcgcgag cgatgtgaac gctctggcgg cgtatatgct ttattgcgtg
     8461 gtgtgctcac tgaaagtgaa acgcaaaccc tactgcatca tgtcggtggt gcgccgttct
     8521 atgttccccg ctgtgataga gcacttcgag cggcacgtaa tgcacgcttt ctcgtggata
     8581 ttgcaaaaga acaaaaatca ggtcgctcac tgcgccaagc aatggccgtt ttatgcccta
     8641 gatatggatt tagtgaccgg tatgggtggc gacttctccg gcagcaatct gacagccaac
     8701 aactatcaag ccccgcacaa gcggggcttt ttgattaata gaggagagaa atgataccgt
     8761 ttaggcttat ttccttctaa tgcccccttg ttcacgctga accctatcag tccataactg
     8821 ccaatccccc tccctatact ggtaccactt gttcgaggtg gtatcggtat gaatggcaac
     8881 acaaccagta ttggctctta tgtgatgtcc tttatcaccg tcgtgatcag tctgtttcgg
     8941 cctgaagagt ggatggtgat cggcattgtc gtcggtattt tgtctgcgat ttttacggcg
     9001 gcggtgaact atttttttcg cgctaaagag catcgtctgc gtcgcgaaga gcatcagatg
     9061 cgtgagcgct actaccgccg tcaccttcac agcaatgagc actcaggaga gcaaccatga
     9121 gtatgcgtaa aacggttggc ggtgcggttt gctcaatcgc agtgattgcc gggctagttg
     9181 ttgagttgta tcccgccgac attcgcacct caaaagaggg attgatgcta attggtaatg
     9241 ccgaaggttg tcggcgagac ccgtataaat gccccgctaa tcgcttaacc gtcggtgtgg
     9301 gtagtacgag agatgtgacg cctggcaagc gttataccga tgaagaaatc gcccaattgt
     9361 gggtgcgcga tatcaaagac gccgaagatt gtgtgaatcg ttattttaac ggcaagcaca
     9421 tgcctcagcg cgcctttgaa gcgatgactt cgttggtgtt taacgtgggg tgctacggtg
     9481 tgcgctggaa ccggaaaaag aatcaggcaa cgcagattta tcgtgaggct caagcgggca
     9541 attggtcagc aatgtgttat cgcatgactg atttttcata ttcagccggt aaacctattt
     9601 tgcgtcctcg acgtgaagaa gaagtggctt ggtgtttagg taaatgagtc gcgtatcgat
     9661 ttgtttatgc attgcgttat tggcggcggt ttgtggcttt aagcatgtgc ttcaacagcg
     9721 cgagtctgca atcaacgcgc tgaatacaga agtggcgcag ttaaaggctc gtaatacaac
     9781 acttacacaa gagcgagatc aagccagagc ggctttaagc ctgcaagcaa agcaaataaa
     9841 ccgcgcgaat caagttgccg aagaggcgcg ccaagcgcgt caaaaagcag agcttcgcgc
     9901 tgagaaaatc cgtcaatcac tgcatacagg tatctcaaca tatgcgtgta gtaatcaact
     9961 tttgcctgct gatgatgctg agcgcttgct ccgctacgtc acccacttgc gtgacgaagc
    10021 cttacatccg gatgccaagc gaactgctcg ccccaacact gccagcgctc ccaccgagtc
    10081 gctgacctgg ggacaagcca ttgagtggat accgctgttg ctcggtaacg tggagtcctg
    10141 caatcgtgat aaagcggcgt tgcgccgcat tgacaaggaa aaagccaatg aaacaacgca
    10201 agcgcaataa agtagcaaat aaacgtgtag tcacgcctgc acgtatcgag catgaacgat
    10261 acacccattt aaacgcacga ttagaccgca ttgatgcgcg tttatctcgc cttgccgtac
    10321 cacatgatat cagtgaacaa ctgtcgcagt tagatgtgca gttaagtgag ctttactcag
    10381 taaccaaaga gagtgccatc aaatatggtg ctgttgctgg ggctatgtcc ggtggtgtgg
    10441 cggggttgat ggtatcaacc gcttttttgt tgctgcgctc taaactgggg atgtaatggc
    10501 ctacccacaa gatacgcgcg accggttgcg gcgagcttat atctataacc aatggtcatt
    10561 ggaggtgacg gcttctcagg ttggggtcag cttcccaaca gcacgacgct ggaaaaaaga
    10621 agcgcaagac gctggagatg attgggacaa gatgcgtaca gccaatctca ttgccggtgg
    10681 cggcatggag gatgcggggc gtgcggttct tatgagtctg gttactcagt gtcaagcggc
    10741 aaccgaagcc atcaatggcg cggtagacat gccagcggat aagcgtgttg aactgttagc
    10801 cagccttgct gatgcgttca acaaagcgac ctccgccagc aaaaagattt tgcctgaaac
    10861 taatcgttta gccattgcta ttgaggtgct gcaagcgttg ggtgagcaca tcgagcgtaa
    10921 gcaccccaat cataaagcag cgtttgtcga tattttaggc tcatttgccg aggtgatgga
    10981 aagtgatttc ggataaagaa gaattggagt cgtggctgta tctcttgcgc agcactgttg
    11041 ctgatggaac ccccgaaggg caggcagcag tacaggcctc tacagaggcc acccgtgctt
    11101 ttatcgaaaa gttaaagacg gattacccag ataaatccgg gctgtttggt gggcttattg
    11161 ctgcgctgga atgtgctgtt caagaaatgt cggactaagg agtaaaacgt ggagcgagtt
    11221 gttttaactg tagatcaaat taagcacttg gcatcgtttg ctgggcttac agtttctgcc
    11281 cctaaagcgc ttaatgtcga agcgcagtac atcatctgtc acggtgaagt gcctgcattt
    11341 gagtcggatg aaggggaaaa aatcccagct tatagcggac tgattgcgta caccgatgac
    11401 gtatcggata gcggagtatt ggagttggat atttgatatg cggccctaga gggccgcaca
    11461 gagtgaatat caaaacgcgc cgcgcttgtt ggggttatag gcgacggtca tggtcatggg
    11521 gatacgttcg tttaataacg tagccccgca tgtattgcag tggagggtgg tatggctgtt
    11581 ctgtggcgtc catggttgaa gtaccgattt gatttgcttg tggtagcaat ctgcgcacca
    11641 gtagtgcgtg gggtcaatgg cattctcgtc tggcttcgtc cgaaatacta tgcttccagc
    11701 tggcagcttg aaaagctcgt agggttctgc gtcatcaagg tggcgtttaa gttgtataag
    11761 ttcttcttca atcgcctctt tttcgcgcgc gagcgcatca taacgcaccg attgagttat
    11821 taattggtct tgcaacaggt tgagttgagc gtaaacctct ccaacctctt tattcgtggc
    11881 aatgctctgt tttagctcca acgcgttttt taacgcgtca atggaggctt ttattccagc
    11941 tattgtgcct tttatatcaa agctcatcat ttcaatttct cgttaataga taaaactcaa
    12001 taaaaacaac acataaaggc atcttggcaa tgccgttagg tggttgaaat ttatcatata
    12061 gatagcgaga aaaaagaggc gagctagctt aaaaaatggc ctccgtttta aggaggccat
    12121 gagtcaattg attatgtctt tgctattcgg ttgtatcgat gaaaagcaat cgctcaaaat
    12181 tctccaaaaa agcgttaatg gccgagttgc aagagtatgt tgctaacttg cgccaaacca
    12241 ttgaggcgga gtgtctaggg tttgacattg atcctgtcgc tagcgccgag cgtcgccgcc
    12301 aagtggctga tgctgtgtcg gggtacgact tctttgtttc cacctacttt ccgcattaca
    12361 tccgtaatac tgcgcgcagt cagctgcaca attacttgtt tcagcgttta cctcaaatgg
    12421 tggcaagtac tgtcggtgaa aatgatgcca tagctgcgcc acgtggtgag gctaaatcaa
    12481 cgctcgtgac gcaactcttc accttgtggt gtgtgattcg tggcataaaa cactacccag
    12541 tcatcattat ggacagcatc gatcaagcct atccgatgct ggaagccatc aaggctgaac
    12601 tgacatttaa cccccgttta aaacacgatt ttcccgaggt ctgcggccaa ggtcgcgtgt
    12661 ggcaaatggg taccattgtc acggccaata acatcaaaat taccgttgct ggctcgggta
    12721 aaaaattgcg ggggttacgt catggccctt atcgtcctga tttgatcgtg cttgatgata
    12781 ttgagaacga tgaaaatgtg cgtagccctg aacagcgcga caaactagag gcttggctta
    12841 aaaagaccgt gttaccactc ggtgaagcag gcggcaaaac cgatgtgctc tatatcggca
    12901 caattttgca ttatgactcg gtgctgtccc gaacgttgaa taaccccatg tggcgtactg
    12961 cacgctttaa agccattctc caatggccgg tcaatatgtc gctttgggat cgttgggaag
    13021 agctattgcg caatcagcag gtcgaggccg cagaccaatt ctaccgtgag cacgaagcgg
    13081 aaatgcgaga aggtgcggtc gtctcttggg cggcgcgtcc tttgttggcg ctgatgaaaa
    13141 tccgtgtccg tgacggacac agcacctttg actcggagta ccaaaacgat cccgtcagtg
    13201 gtgacgatgc aatttttgcc gcgccaggtg tacttaaatt ctggaaagaa gaagtacgcg
    13261 agtgggtctt ttacggtgcg tgtgacccta gcttgggtaa gttcggcaac aatcgccgag
    13321 atccatctgc aattttagtc ggtggtttta accgtttgac gggggtattg gatgttgtcg
    13381 ttgcagatat ccggcgtcgt gtacccgaca aaatcatttc tgatgtgatt gagtatcagc
    13441 gccgttttgg ttgtttagcc tggtctttcg agtctgttca atttcaagag tttttgcgta
    13501 cagaactcgt caagcgctct gcgcaactcg gtgtgcctgt tccagcaatt ccggtgatcc
    13561 cgtcatccga taagttgtta cgcatcgaat cgctacagcc acacattgcg aatggtttga
    13621 ttcggtttaa tcaagaaacc caatccacgc tcttagatca gttacgtcat tttccaatgg
    13681 ccgatcatga tgatggcccc gatgcactgc atatgctgtg gtcactggcc gtatcgcgtg
    13741 cccactcaac gcagattttc actcgttcac gacacactta cagcggcgca cgcttaggta
    13801 gcggcgcttg gtgaggacgc tatgccacaa attgttgacc aattcggtaa tcctcttaaa
    13861 aaagaggcat taaaaacccc gcaaaccatc aaaatggcgg ggacgtatca tcaatatcca
    13921 gagcatctct ccaaagggat gactatcagt aaactgcctc ggctcctgcg gcaggctgag
    13981 ctaggtatgc tcagtgcgca agcgagtttt ttcgaggaca tgctagagcg tgatgggcat
    14041 gtttttgccg agatggagaa gcgcaaaaat gccctgttaa cgcttgagcg cactatcgtg
    14101 ccaccggaga atgccagtag cgtcgagaag aacgccaccg ccgcagtgaa agagtggttt
    14161 agcggcatta tggagttaga agatgtgatc ttaaatggtg caacagccat cggtcacggt
    14221 tttagctgtc aagaaatcca atgggtcacc gttgaaaatg tgctcattcc tcagcacctg
    14281 catttacgtc cgcactattg gtttaactcg ttgccggaaa aaggcgatga gctgcgactg
    14341 aacagcacta atccagacgg agacgtcctg tggccgttcg gttggttagt acaccgcgcg
    14401 aatgcgcgta gtggttttgt tggctctagc gggttgttcc gcgtgctggt gtggccgtat
    14461 ctctttaaaa actttgccgt tcgtgattta gccgagtttt tggagattta cggtctgccg
    14521 gcgcgtattg cgtattacgc accgggcaca tcagatgaag accgtgacca attgctcatg
    14581 aatttaattc gtttagggca tgatgcggta gccgccgttc ctacgggcaa cgaaattaaa
    14641 tttgaaagtg ccgctacagg gggaagcgat gcgtttatgg cgctgattga atgggccgaa
    14701 cgcaccgagt ccaaagctat tctcggtggt acgctaacca cacaggccga tggtaaaacc
    14761 tcaaccaatg cccttggtac cgtgcataac gaagtgcgtc atgacctgca ggccgctgat
    14821 gctcggcaga tggaggggat gttccggtcg cttattcaga tgatgctggc attaaatggt
    14881 tttggtgata ttccgatcca ccgtcagccg cgcttggttt ttgatacccg taaaaaagca
    14941 gatttaagcc aatttgcctc agcggtttca acgctggtta atgcgggctt ggataccatt
    15001 ccggtcagtt ggattcaccg tgaaagcggt atcccattac cgcaggatgg tgaagctgta
    15061 cttactccac gtcaaacagg gctagccaac ttgcgccaag cctatccagt gagtcccggc
    15121 gtgggggttg cggcgctatc gcaaacagag gacacgccag aaagtgatcc gggccaacaa
    15181 gctattgata cgatgccgcc gctctcggat gcggtaggtg atgcgatgaa tcagctatta
    15241 gcccccatga ttgccgcgtt gcaacaaggc caaacagccg atgaggcaat gaatatccta
    15301 gcagaggggt atccgcagct ggatgataca cagcttcgcg ccttactcac gcaagctatt
    15361 tttgtgacag acctttgggg gcaactccat gccaacagct gagaaagtcg atttggcgta
    15421 tgccatcggt ctgtcgccag cggaggccgt ggcttatttt gagtcaaagg ggtatgtcat
    15481 cggtctgcat tggtacgacg tctatgcaga ggcacatgcc aaaggtttta ccgttgccgg
    15541 ggtattaaag ctcgacatct taaaagacct acgcgagggc ttacaaaaag ccttgcaaga
    15601 agggagcacc caagttgatg tggagcgcca actgctccct attttggagc gtaaaggttg
    15661 gttgggtaag ggattggtag ttgatcaatc aacgggagag ttattgggta aacgcctgat
    15721 gccgcgccgt ttagatacga tttttcgcac caatatgcag tcggcttaca atgcggggcg
    15781 ttatcgtgag cagatggcta atgcagccag tcgcccctat cttgagcgtg tggccgtgat
    15841 ggatagtcat actcgccctg cgcattcacg gttaaatggc tttattgcgc ccattgatag
    15901 ccctgtttgg ggctttcttt atccgcctga tgcctatggt tgccgttgtc gtgttcgcgc
    15961 ccgttcggcg gctgaggtgg agaagctggg attaaccgtt cacaaccctg acgttatcga
    16021 aattgaacaa gagtgcggta ttcccggaga ggtgcgtaaa gtgcctgcga tgatcaatcc
    16081 tttcaagggc accgtgtatg cagccgatcc cggatttggt atcaatccgg gaaaagtctc
    16141 gtggcagcca gagttagata agtatgcaac acctgccgcg cggcaataca ttacggcaac
    16201 gctcacaggc ccagatttcg cgcgtagtta taggaaggct attgcgggca cagtggacaa
    16261 acaacagcgc taccccgtgg caatgcttgc gaaccccatt gatggtagtc gtgtcgtgag
    16321 tgtggatggt gccaccctgc aaaacatggc tacaggcacc cccacggtga catcagagat
    16381 gtttttattg gctcaacagg ccattcaaca cccagcgaaa accgtggagc aacaaggtgc
    16441 aacatggtat tgcgtgatgc gacagggtga atggtgggtt gctcatgttg cccacggtca
    16501 gctgattggc ttaacgcatg ggacggattt agatgcgctt ttgccggagg tgtaaatgct
    16561 aacggtagac atagatacgc gcggcgttca acgccattta aatcatcttt tacagggtat
    16621 taaacaccgc caacccttaa tgcaggcatt agcgggtgat atgtggaacg cggtagaaga
    16681 gaactttaaa cagcaaggcc gccccacttg ggctggttgg agtccggctt atgcagcgcg
    16741 acgagggccg gggcaaatat tgcaacgcaa aggccgtctt gctgccagca ttgtgccggt
    16801 tgctgataat cagatggcgc gggtcggtac aaatgtaaag tatgcggcta ttcatcaatt
    16861 cgggggcaca attaatatgc ctgcgcgcag ccaacgtgcc tattatcgcc aacgtaaaga
    16921 tggaacgatt ggaaatcgtt ttgtgcacaa atcgcggagt aactacagcg aatggcatac
    16981 gcttcccgcc tataacattg agatccccgc ccgtccattt ttgcaattaa ccgacgagga
    17041 catctcaacc ttggaggaga cggcgcagca ctatttacaa ggggtaattg atggaaatcg
    17101 ctaaaacgcg ctgtaagcga ttctgaggcg ttttcttgtt ttgtggtgtc atcgttcagg
    17161 tgacaccctt tcaaacaaat ttaaatggtg tttaaatgcc ttcctgagcg tcttcttttc
    17221 ttagcatccc ttttctgtcc ttttctccac taaacagcct tgctgaaccc actcaacgct
    17281 taatcccctc ttgtgatttg tagactagcg gcatgaaaac acgtcttgct gctttatcag
    17341 ttgaaatcgc tgccggtgcc gagcctgtct cccgtttgcg aatcttgcct gcgggcgagt
    17401 ttcgcggggt ggatggtcgt cctcgtgagt gtgcagcgtg ggtcatgacg gcaacggccg
    17461 ccgcccgcat tatcgaaacc gtcaattctc gcgtgacaga gctgtgcttt gactatgagc
    17521 accagacatt gcgtgccgta gagaatggca agcctgcccc agctgcgggc tggttctctc
    17581 agcttgaatg gattgagggt gaaggtttgt acgccacgga tatccgttgg actgagatag
    17641 ccaccgacat gatccgcaaa aaagagtacc gctatatctc gccagttttt ttctatgacc
    17701 aagtaaccgg tgaggtaacg gcactggtca acgttgcgtt gaccaatacc ccagccctag
    17761 atgatttgga tgaagttgcc gtggcggcgc tctcccgtct agcggcgtta tcaacaacat
    17821 catctcaatc ggagggtaac acgatggatg aagagcagat cgcgaatctg ctggccgatt
    17881 tacgatggat tttaaattta ccgaaaacgg ccacagctga agacatcaaa gtcgaactgg
    17941 acaaggtgat tgcggccatg agcgataacc aaggtgttgc agcggccagt cagggtctga
    18001 tgccgtggat cgcgcaacag ctgtccgtaa aagatcaggt tattgcgact tgcaacacgc
    18061 aaatcgccag cttatctgcg gctgcatatg acccaaccaa gtttgtaccc attgaggcgg
    18121 tcactcagct gcgcgcgcaa ctgagtcaga ttcaaaccca ggcagccacg caagagttgg
    18181 atggtttggt tcaggctgcg ctgtctgatg gccgcttagt gcctgcgatg gcggattggg
    18241 caaaagagct ggggcgtaaa gacatggccg cgttgaaaac ctatttagac ggcgcacctc
    18301 gcttagctgc tcttagcgcg atgcaatcca acaaaacgga gctgaatgca ccacaacatc
    18361 gccaagatga cagtgcatta gatgcggccc aattggcgat ttgctccagc cttggcatcg
    18421 aggcgagcga tatcgcaaaa gagttgggag aaatctaatg gatcgaaaca caccttttaa
    18481 tgacggcaaa ctgttccccg tgccctgtga agcgggcgca aaaatcgatg tcggcaacct
    18541 cgtctgtgtg aatagtgccg gttatgccat tgcaggggcc aaaggcagcg gtaatacgtt
    18601 gctggggtta tgcgatgagg ccgttgataa cacaaacggc gcacaaggtg agcgtgtggt
    18661 tatggtgcgc cgtggtttgg cggtgttgct ggagaaccat agcggtgatg ccgtgacgca
    18721 agcggaagtg ggtaagccat gctacctgca tgacagcgtt actgtgtcaa aaacggacgg
    18781 cggtaatgcc ccattggttg ggcgcgtgtt gaccgtctct gctcacagcg gtgttcaagt
    18841 tctcattgat taaggtacca acatgctgat taacaaaacg aatgtgacga cgtttttcgt
    18901 cggcttaaaa aagctcttta acgatgcgct aaaacgcgag ccgggtcagt gggaaaaaat
    18961 tgccatgaaa gtcccttccg gcaccaaaac agaagattac agctggttga gtgacttccc
    19021 tcgtatgcgt aagtggatcg gggagaaatt tgtcaaatcc ctgtctgcat tcaagtacag
    19081 catcaccaac gatgactggg aaaccaccat cgaagttgat cgcaacgacc tggaagatga
    19141 ccaaattggt cagtatgccc tcaaggcaaa aagtgcgggt cgtgcagcag ctgatctgcc
    19201 ggacgacatc gtttttgctc tggtcaatga tggatttaaa accacctgct ttgatggtca
    19261 gtatttcttc gataccgacc acccacaggc aggcggcacg gtatccaaca agggcaccaa
    19321 ggcgctttct tgcgctagct tggctgaggc taaagcctca tttggtgccg ccaagatgca
    19381 aatgcgccgg tttaccgaca gcgaaggccg ccctttgagc attcgcccaa ctgtactgct
    19441 ggtgccgcct gagctggaag acacggccaa cacgctgatg acagcggatc gctttgaaga
    19501 tggcaaagtc aatatttaca aaggctcgtg cgaagttgtg gtggagccgc gtctgacatc
    19561 accaaccgcc tggttcctgc tggatacatc aaatgacgtg aagccgttca tctaccaaga
    19621 acgcaaagca ccgaagttag tagagcaaac cgacgaagtt gcggatgacg tatttatgcg
    19681 tcgtaagttc aagtttggtg cagaagctcg tgcagcgggt ggttatggct tctggcagat
    19741 ggcatacggc tctacagggg aggcttgagc atgatttata tcaccgctaa acgaaacggg
    19801 ttttatcgct gcggtattgc tcacagtgac cagacaactg cgtacccaga tgatcacttt
    19861 acaccagaac aattagcggc attagaggcg gagccgatgt tgattgtgac ccgcgacaat
    19921 ccgcaaacgg caacccaaga ggccgcagag cagcaaattg ccgcattgaa acaggcattg
    19981 acgcaaacgc agcaacacaa tgccgagttg cgtgaagagc tggacgcagc acatgcacgc
    20041 cttgctgagc tgacaggcga tgcaaaagta accaccccaa aggctaaggc gaagtaaggg
    20101 ggcgaagaat gtacgcgaca cttgaagaca tgctgttgtc atttggtgag cgcgaatgtt
    20161 cgtcactgtc tgaccctgat tattcagggg ggattaatga ggcggtcatg gcggccgcct
    20221 tacaacgcgc gagcgcagag attgatggtt acttagtcgc gcgttatccc actccgtggc
    20281 cggatacccc gcgtattttg gtggggcgat gttgtgacat tgcgcgttat cacttagcta
    20341 ccgcctcccg gcaatgcacc aatgaaatcc gtatgcgtta tgaggacgct atccgctttt
    20401 tggaaaaggt ggcctcgggt gtcattagtc tggggcgaac tgacagcggc gaccccgtca
    20461 aagctgggtc acaaatgcgt ttcatgtcta caccacgcca atttgggcgt gatgcaacag
    20521 gaggcggcgc attttgatta ctgagattga aaatgccgtt atagaccgtt taaaggcggg
    20581 tatggggcgc atggtacgcg atgtggtgag ctatggcggt gagctggatg atatcggtga
    20641 gatcgtccgc gcattaccgg cggcgtgggt cacttttggt ggcattacac acactaaagc
    20701 cgtgagcacc agcaataagc gatttagaac gcatgggcgc tttgtcgtga tggtcggtga
    20761 ctacaacgcg cgttgtgagg tggcaggacg tcaaggcggt gtccgcattg atgaggtggg
    20821 ttgctatcgg atgatttatg ccgtgcgtcg cctgttgatg atgcaaagcc ttgggctgga
    20881 tatcacaccg tttcaacccg gaaaggtgcg cacgctcttc aatacccgga tgaatgaaaa
    20941 ggcgctctct gtctttgcca ttgaatttga cacgtcatgg attgaagcgc cgctggcatt
    21001 gggggcgtgg ccgatgcgag aggattgcgc tacggagcca gaccatattt tcacacgcta
    21061 tcgcggaaaa ctggccgaac cagatgcgcc catttcgcgt attggtctct cttatgaccc
    21121 acccggtatt gggtcacgtg agcatccagc agatttaatc aaccttgatg aggatattag
    21181 cgatgaaagt taaagcggca aaagggttgc tggtgccaaa agaaaataac ccattccgct
    21241 atatcgatga tcgagcattt gttgaggtgc cggatagcgc gtattaccgt cgtttgctcc
    21301 agtcaggtga cttacaaggc aaacccgtaa caggaaagac agcaaccgtg gcagaggcgc
    21361 acacagagga taaaaccgaa tgagcataac ttttgacact attccgggca gtacccgtcg
    21421 ccccggccag tacttagagt ttaatactcg catggccgtg cgctcattgc ctggcaacct
    21481 gcaaacgatg ttgattatcg ccccactcat gaaaagtggt acggccaagc cattagagct
    21541 gcataacgtc ttttctgatg atgaagctgc gaccttgttt ggtgcaggct cccttgctca
    21601 cttgatgtcg cgtgcagcta ttttgagcta taagtacctg caattgcagg tgattggaat
    21661 cgacgagcac agtgcaggtc gcgccgcaac aggcactatt actttaacag ggccagcaac
    21721 cggtaacggc tctctgtctc tgtggatctc caatatgcgc attgatgtcg cgattgatga
    21781 ccaagatgat gcagatgcca tgcataacgc actggctgcg gccattaatg ccgattccct
    21841 gttgccggtt actgccgctg ttactgatag tgacggtaaa aaggtcacgc taacggccaa
    21901 gcaaaaaggg gcgtttggca atgacattat gctgttgggg tcatgtaatg cggcaggcgt
    21961 tacgctatca gtacaggcca tgagtggcgg ccagcataac cccgatattc aaccggcgct
    22021 ggatgcagtc tttgccgcag ggcacaacgt gattgttgcc ccgtattcag atcaaaccac
    22081 gctgacggca ttacgtgaac acctgaaaaa aacgggtcat gccatggagc agcgcggcgc
    22141 cgttggcgtg gccggttggc cgggtacgct ttccaatggc accacacttg ccggtcagat
    22201 taatgacgga cgtgtttcgc tggcttggca ttccggctct cgttgtttag cggggattat
    22261 tggagcggca tatggtgcga tggttgcctc agaggaagat cctgctcgtc cgctcaactc
    22321 gttgccactg gctgccttag atatcacccc gctaacggca cggtcaggcg gtacagagca
    22381 agaaaatgcg ttgcacaacg gggttacgcc tttggagatc ggccccggta atgtggtgca
    22441 aattgtacgt gcagtaacaa cctatacccg taatccgcaa ggtgttgctg accccgcatt
    22501 gctcgattta acaacgattc gcacactgga ttacacacgc aaagcgtgcc gtgagcgaat
    22561 tgcgctacgt ttcccgcgag agaaattgca tgagcgcacc cgtgcaaaag tgaagtccga
    22621 gttgctggat gttctgttga agctggaaga agaggaaatc ctcgaaaacg tcctcgcaaa
    22681 caaagaaaag ttgttagttg agcgcaatgc caaagacccg aatcgtcttg atgcggtcat
    22741 tccagcggat gttgttaatg ggctgcatgt gtttgcaggc cgtgtggatc tctatctgtg
    22801 aggcaataag ttatgactca agaatacatg ggcgcgattg tccttgaggt ggatggtcag
    22861 gagatcgaag tcaccagttt tgatgtcaaa acaaccactg gacgtaagct ggttaagacg
    22921 atgaataaaa caggtcgcgc taagggctat tgccaaggga ttgcgaccta tgacctcagt
    22981 ttgtcggtgg ttatcccgga taccggtgat ttagattggg aaaacattgt tgacgcgaaa
    23041 gtcacgctgt accccctgaa taatcaggat aaacggactt cgtatttggg gtgtttctcc
    23101 attgatatca gtgagaaata tcaggtcgat aacgaagcgc agcgtgattt aacaatggct
    23161 gctttgcgga aggtggatga atgagcatga cagtaaaagg ctcactgcgt tacggcatcg
    23221 agcataacgg taaattgcat tacgattttg aaattgggtt gctgacaatg gggcaatcgg
    23281 gcgagtgctt agatgcaaca gagtcccgtt ttggctcttt ggatagccgt caggccgatg
    23341 cgttctatcg cgcggcgagc tttgcatatg ccttacgtca attaggggat atgaccaaag
    23401 cggatatcac cccagaactg ctgtatacgc agctgacgca agacgatgcg gatgtgctag
    23461 ataaagcagt tgacgaggtt aaaaaaaagc gcagcgagcc gaagagcgca aagctggctt
    23521 gcgactcgcc gtcctcatcc tcggtaaata tggcataaca gctgagcaag tatccgcgat
    23581 gagccgtcca gaattggacg gctatctcaa cgcattacag cgttaccacg ccccccgccc
    23641 ggtcggtaaa cgcgaccaca caacaacgac cgttgaacat aaaacgtata aatccttgcg
    23701 taaaaaacga ggttagtgat ggcacgtgat tttaaagtct cgatgacatt agaggcaaaa
    23761 gatgacgcct cgcgccaagt tgcccgcgcc ctcaaagaaa ccacgggaca cgccgaaaaa
    23821 gcggcaaagg ccatcaagca acagggtaaa gcacaacaac aggctgccgc atccgcagcg
    23881 gcggcagcaa aacaacaggc aagggacagc attgcggcaa gccgcgcaca actggatgaa
    23941 gcgcgtcgca tgcagcgcgc acgtgaagcg ttagggattc gctcggaaca agcaatccag
    24001 cgagaaattg accgcaccat tgccagctat aaccgtctga cccgtgcggg cacactgtct
    24061 gcgagagaac aggcgcgggc ataccgtcag atgcaacacc aagttgctga gctacggcga
    24121 gagatgaagg gggtaaccgg tttacaacgt gccagtgcat tttttagtaa aagcggcgca
    24181 attgtgggtg gcgcagctgc ggctggtgct gcattcgtta gcccgatacg caatcgaatg
    24241 aactatgacg aacgcctgac tgaaatggca aatacggcat tcagtgatgc agacctaaaa
    24301 ggtcggcagg ctggattgca gcgcctagat aagatgattc gtgataacgc aatgtccagt
    24361 gggctgaaca aagagtcagt tatggatacg gtcaatacat tgcttgctag cggattaaaa
    24421 gaagatgccg ttagcaagct gctaccgacg attacgcgtt acgcctctgc ggctaaagca
    24481 gaccccaacg aactagctga tattgccgta aaagctcgca gcactttcgg tatcagtgat
    24541 gaccagatgc cgaccatgct aaacatggct atcgcagcag gtcaatcagg caattttgag
    24601 ctgaaagaca tggctaaatg gctcggtcag cagatggctt ttgccaaaaa ggcgggcatg
    24661 tctggactag aggattttgg caaacttctc acattgaacg agttagctgc tgttaccgca
    24721 ggtagtagcg atcaagcggg aaataacgtt gttaattttc tgggtaagtt gacaagtcaa
    24781 gatgctgcca atgccgcagc caatgtcaaa gttagtggta ataaaggcat tgatttaccc
    24841 ggcacgttag ccgaggccag aaaaaatggc atagaccaaa ttgaagcgtt ttctcgcatg
    24901 attgatgaag ttgtgaaaag tgacaaaaag tatcaacaac ttcaagccaa actgaaaacg
    24961 ctccctaaag acgataaaag cggcatcaaa gaaacgctgg aggcgatgag tgccatattg
    25021 gagggggcgg gagtaggaaa aatccttaat gaccaacaag cgcttatggc gatgattgcg
    25081 taccgcaatg gtcaaggata caaaaacgaa attgaaaaaa acatcaataa gcaacgcaat
    25141 cttaagcctg gtgagcaagg tgctggcgat gtgaattttc agttcaacaa agaacagggg
    25201 tggtttaaag cacagcaatt taagaatgca aaggagtttg cagagcttga ttctatcaag
    25261 tcactatcag atgcaacagg agatgcagcg ggaaaagttg ctgaatgggc agagcaatac
    25321 ccaacactaa cagcggcagt cagcggtgcg actacggcta ttcaagggat gacgcaagcc
    25381 gccattgctt ttgctgggat taaaatctta acgggaggct ctgcgggcgc agcagcaggc
    25441 gcggctgccg ctggaagtgc tgccggaaca gcggccaagg cgggcagtgc ttttggctgg
    25501 attagtaaag cattagggcc tgcaatggcc gtaaccgccg cagccacatt taccagtgcc
    25561 gaagaggaag atgagttact caacggtgaa gctaaatgga aagcgttgcg agaaaagtac
    25621 ccgcaagagg tcatcgataa agcgcgtaaa aaatatcaac cgtggtatca gtttgggcgc
    25681 ggatacgcga cggaaaacga gcgctggatt aaacaatatc tcgaaacaga aggattagga
    25741 ttgccgactc cgcaacacgt catgaatgcg attcaacctg taaccccgca atcaacaacg
    25801 gcgactacac cacccccaca gccgaccgca ccaagcagtc aagtgatccg cttagaagtg
    25861 gatggtcagg ttttggctga aactgttaat cgcttcaatg cgcaggaagg agtacgggga
    25921 tgagttggcg aaacaaccta caggatgcct cctttaaggg gctgcgtttt gatgtcatca
    25981 aggaaacaac cgcgcacagt cgggattacg ccgctcatga gtaccccttt gtgaatgggg
    26041 cagatatcca tgatttgggg cgtaaggcac gtaatttgcg cttaaccgcc gtattttggg
    26101 gggaggatta tgattaccgg ttacaaaatt tccttgctgt gctggacagt gcgggagccg
    26161 gtgaactaat ccaccctatt tatggctcta ttccacacgc acagcccatc gaatgccaag
    26221 tatctcacga agcagataca ccggattact gcaccgttga gttggtcttt ttagagtctg
    26281 gggcaggtaa aaacttacag cctgtggttc ggcctgagca atggggcgac accttattta
    26341 attcgctaga ggcgttacag ggtgatgtta tggcgctata cgaggcaggt atggctccca
    26401 ttacacgcac tcggcggttg ttgaccaagg gaaaaaccgc gctttctgcc atgttgaaca
    26461 cgctcaccat catgcgtagt ggtgtgaaag gtgtctttac ggacacagcc gactacttgg
    26521 cctttccgaa acgctatgtg aatgatttgc gcgccgtgtt ggatttacgc accttggcta
    26581 cagacacagt cttaaatcgt caacatgcac agcgtaccgg ctctacaact gggcgaattt
    26641 atggtgatac tcatgttgta tttacgccac aaggtgcgaa tgcaaatgat gacgtcatgc
    26701 agaatttagt ggcttataaa gcgggattaa ataccgttca aacagcactt aaagaccctg
    26761 cgccgttgtt gtccgcatgg aaacgtgatt gcgggatatt agagcaagtt atgcagttcc
    26821 cggtggcgtt gttaaatcgc agtacgacgg cagttgttcc catcccagac acagccaaaa
    26881 tggatgacgt gtgcgatgtt gtcaccttgc atacctcgct tgccgcgatg cgtgcggtcg
    26941 agatctttac cgacgttcta actgatgacg cagcgcttgt gcttactcca acgcatattg
    27001 aacaaatggc taaccaagca cgccagtaca tccaaaacgc gcttgagtta aatcgtgaaa
    27061 cttataagcc atttactaca gcaatcagcg aagaccctga tgcgaggggg ctattgtggc
    27121 cgcccgttaa cgagcggcta aagtcaattg cattgggggt tcaggtgtta gccgagcaac
    27181 aaatcacacg tcgtccacca ctggttgtac gtgtcgttac tcacgatacc aatctgcact
    27241 tactagccca tgcatggtac ggcgactacc aacgtgcccc ggagttatta cgtctaaacc
    27301 ctcagttagt tgatccgaat gctattaagg cgggagataa actgaatgct tacagtcgat
    27361 aacgataaca tcacactgct gttggctggc aaagcataca gtcattggca gcgttaccgg
    27421 attgactcgg atttcctcaa gccggccgat ggttggcagc tgggattagg gttgccgtca
    27481 gggacatttc ccgcagatgc ggtgcgaggt gctccggtta aggtcatggc tggggatgac
    27541 gtcattctgt ctggacgtat cgatagtgtg cagcgtgagg tatcacggcg aggcctcgtg
    27601 ctgtcactca gtggtcgtga taatgctgcg gtgttggtcg attgcgccgc gcctatttat
    27661 aacgcgcgtc agttaaatct tgatgaagcc atcagcgcta ttgtctcccc gctgggtatt
    27721 aaacgggtac gcattcaagc atcaagtgtg acacgcaacg atatgatcca tattgaaccc
    27781 ggtgaacgcg cttgggatgc tttagtgcgt gttgcgtctg cgcgtgggtt gtggccgtgg
    27841 tgtgatccag atggaacact ggttattggt ggcccagatt acaccaaagc ccctgttgcg
    27901 acattgcaac ttagtcgcga tgaaaacaaa acgaatgtgt tatcgctcgt tgataaccgc
    27961 aatatcagtg gctgctattc agaattgacc atgttggcac aatcccatgc cacattggcg
    28021 gatagtcagc ccgtcgtcat cgatgtgact gcaccccaca gcgacacgga aagcgcagac
    28081 acattatcga gtggtgaaac tggccgctat aaccgctctc acaccgagaa agaccccaca
    28141 gtccagtggt atcgtccaca aattcttgtg gtgggcgatg tcgataatgc tgagcagtta
    28201 gcgtatcgcg cccgtaaagc aatggctgat gcgcgtttga gcggtttaga tatcactatt
    28261 gaggttgcag gccatcgaac ggcatctggt gaactatgga cacccggcca gcgcattcac
    28321 gttatcagtg agttgcatgg tattgatgat gtcttttttc tcatgggacg cagtttaaat
    28381 ggcggacgtc ccagcggaaa aacaacaata cttcgattaa aagaggatgg cgtctggcta
    28441 cctgatgcgt atccgacaga taagaaagca tcacgcagga agaagaaaaa accagagctt
    28501 gtagtcttgc ctgtatggga gaaaaattaa ccatgtggaa tgaggtcaat cgccgcatta
    28561 gccaagcatt agggcgtatc cgtctcgctt ttcgcatcgc cattggtagc gttgacagtg
    28621 gtgctaaagt gcaaacaatc caagccgcag gaattggcgg tgagtcgttg cgtggtaatg
    28681 agctattcca gcaatacggt ttcacgtcgt gcccacatcc gggcactatg gggatagtat
    28741 tgcccttggg tggagtatca acgcacggta ttgttatcgc tacagagcat ggcgcgtatc
    28801 gtctcaaggg attaaaagcc ggtgaagttg cactgtacga tgatcaaggg acgaagattg
    28861 ttcttaaacg cgggcgcatt gtagatattg agtgcgacgc tttccgcgtt aactgcaaaa
    28921 actatgaagt caatgcagag aaccgagccg actttaacac cccctcactc aatgccagcc
    28981 aagcgttaac ggcggaggga aaaattagcg gtaatggtgg tatcggtatc aagggtggca
    29041 aaggcgctga atttgaaggt agtataagcc agcgcggtgg cagttttagc acggatggtg
    29101 atgtgagaac gtctcttgtc tccgtgaacc accacgccca caccaacggc catgatggcc
    29161 gacctactgg cgatcctatt ccccagtaac gccttgctga actctctcag cccttaattc
    29221 actgtccatg cgagcatgat tgacgcatgg acagacttat ctctcccaaa acaggtgatt
    29281 acaccggcac acgttgccgg tcattagaga acgccgttta tttacgcctt aaaacccctt
    29341 tagggagcta ttgggcggat aaaacacttg gttctcgcct gcatgaatta gcacgagaaa
    29401 aagacctcac gcgcgtgcat ttattagcac ggcaatatgc cgaacaagcg ctgacgccac
    29461 tcatacgaga tggacgtgcc agccatattg ccgcaacgac acaaaaaagc cgtgcgggat
    29521 ggttaacact ccaaataacc gttgttgatt ctggtgggga tgagtattgc tttgaacatc
    29581 ctgtgagagt gatggcgtaa tggcttttgt gtatgaagat atggcgacta tccgtgagcg
    29641 acagcttcga gatattcgca atcagttgcc tgatgaagat atcgatactg ggtcagatag
    29701 cgatcatttt gtacgtgcat ctgctgttgc cagtgtggcc gaagggttat atcagcatca
    29761 ggcttggatt gttcgccaga tttttcctga taccgcagat cccgattatt tgcgactgca
    29821 tgcacggctt cgagggcttg agaaaaaaga ggcaacctac gcagaaggta ttgcaagcgt
    29881 cacgggcact ttaggggcta cgctaaaagt cggcgcagta attcgcaatg caactcgaac
    29941 ctgtcgaaca atagagcctc ttaccctggc tgagggtgtt aatactgtca aggtgcgagc
    30001 agatacagcg ggtgttgctg gtaatcttgc tgtcgcagag agtgccgagc tggttagtgc
    30061 tccgatgggc attaatagcc gtgtgcagat tatgcaattg tccggtggca cagatgatga
    30121 atccgatgct agcttgctgg cgcgcttact ggacattatt cgccgcccac ccgcaggagg
    30181 taatcaatat gattatcggc gctgggcatt ggaagtcccc ggtgtcaccg gtgcttatgt
    30241 ttatcctttg cgccgtggcc taggaaccgt tgatattgcg attacctcag caggcgcttt
    30301 gccgtctgaa agtgtcatca aagcgacgca agcacatatc gatgatgtta gaccggtcac
    30361 tgcccgcgac gcattggttt tagcgccaac gcaaaagcgc gttgattttg atatcgcggt
    30421 tagcgttgat ggaattaccc ttgaatcagc taaaacaacg attagcgaaa caataaaaga
    30481 tgccatgtca cgcatcaatc cggggcaacc attaattcgc agccaaattg aaatgctcat
    30541 ttcattaatt cccggcatca ctgaccgacg aattattaca cccgcagata atgtgattgc
    30601 gcgtgttgat aaatcacatt tggaatggtt ggtatgtggc tcaattagtg ttaggagtgg
    30661 cgtatgagcc ataagcaact attaatggct cttatgcctc ctgtaagtta cacgccaaca
    30721 gagaaaaact tacgggccga gttgaccgca gagggcaatg tttttgatgc ggtcaaacaa
    30781 cgagctgaaa gcgttcttgg tgccgttacc cctttctttg cgcaagagtt actgccggac
    30841 tgggagcgtg tattagatgt gcagccgcag gctggggaca cctatcaaca gcgtttaagt
    30901 cgagtacttg ccaaaatcag tgaaacgggt ggattaagca ttccttactt tatccggttg
    30961 gcggcaagca tggggtatca aattaccata gacgaactcg atgcttttcg cgctggcaga
    31021 aatcgtgcgg gagatgtggt ttatgcgccg gaggttattt ggatatggcg ggtgaatgta
    31081 tttaattctc gggtgcaaac ttatcgcttc cgcgcaggcg gctctgtagc tggagagcgg
    31141 ctttcttatt ttgctgattc aattattgaa ttgatattta atgacttaaa acctgcgcat
    31201 acgttctgtt attttaccta tcagggataa gttatgcaag atttaatgcc cacaatcaat
    31261 acgcctgata atgtctttca tgacggaaat gatgctacag gcgagttagg tacagttgta
    31321 tccgccttgt ggctgaataa cattcaatca gcggtgcgtg atattcaagc agaatgccac
    31381 gccatcttaa cggcgaatgg ttttacgcca gactcatcac gacaaaatca gttattgcaa
    31441 gcaattcaaa aagcaataac aagcaaggtt ccatcagcat caacaagcca atctggattg
    31501 gtgcaattat ccagtgcggt gaacagtgat agtgaaacaa cggcaggtac atccaaagca
    31561 atcaaaaccg caatggataa tgcaaacgct cgtttagcta aatcgcgtaa cttgtctgat
    31621 ttagataatc cagcgcaagc ttgtcagaat ttagggttgt cacaagccat aaaaaacgct
    31681 gacggcgcta tgcagaagaa tgctaatggc gctgacatta atgataaaca gatttttatt
    31741 gataatctcg gtttgcgaga aacggttaat aaggccagta acgcctatcc gggagtcaag
    31801 ttattggatg cgccaccagg gaaagaatat attggtgcgt tctcctgtag ctcacaaggt
    31861 cagcatgtca aagggatgaa tattggtgtt caatcgtcgg attgcgccca agttttagtt
    31921 gacgccaatg ctgagttacg aattaagttt tttaatggta atggtgctgt aaaggaaaat
    31981 gccattttta atggtacgcg agcatttaat acccttcctg aaagggatta tgctggggcg
    32041 tttacttgcg gcagcaatga tagttacgcc aaaggtatta gtattggtgt gggtgccgat
    32101 agtgatgttg gacaaatttg gctagattca tcggcgaatt tgcacacgag gttcttaaac
    32161 tctaacggtg atatccgacg aaataaaatc gttggtgtgc ctattggcgc aacgattgag
    32221 tggcagtcta cagcgcctat tccggcaggt tttttagaga ataatgggcg ttcatttagt
    32281 gcacccgctt atcctgaatt ggcaaaagtt ttccctaatc tgaaattacc cgatgaccgc
    32341 ggtttgttta aacgcggtct ggataatggg cgcgggattg attcggggcg gtctattggt
    32401 agtgagcaag gggatgccat gagaaacatc tccggctcgg ttggtggcat tactgcagaa
    32461 tcatctggcg ttgcggtgtc tgggccgttt tcctactcat attcgagtgg cggacgagct
    32521 gctggctctg gtgcggggat gctgggattt acatttgatg catcacgagt agtgcccgtg
    32581 gcttcagagt ttaggcccaa aaatagagct gttatttata ttacgagggt attttaatat
    32641 gaataaatac agtgttgata ttatggatgc tgtgtttgat gacgcgggta ttgcggtaaa
    32701 gtcaggttgg gtgaaaatct atcattgtca tcctgtaaca cgcgaattta tcggtgcagg
    32761 tatggatttt gtacatcaag ggttttctat acccgcagac gcatatctgg atgcaccaca
    32821 tttacctgag cagactaaaa atattgctat ttgtcgtaat gtggatggca acgcttgggt
    32881 tgcccttgtg gattatcgag gttgcacagc ctataagacc gcaacaggtg agccgattgt
    32941 ggttgatttt atcggggatc ttccagagac gttaacgcta atagccccct ctagcccatt
    33001 tgaccattgg cttgatggac aatgggtgac ggatacctca gcgcaacatg ctgctgcggt
    33061 gtcagaggct gaaactaaaa aaacaagtct acttgctgag gcttccacaa ttattgcacc
    33121 gttatctgat gcattagctg gcggctatat tacggatgag gattcaaaga agttactggc
    33181 atggcaaaag tatcggtaca gcctaacaaa agtcgatact agcttagcgg aacagactgc
    33241 atttccaatg aaaccaagct gataactctt ttagagaaag ctaaataatc cactcctgaa
    33301 cattgtgcta ggtgaaaaat tcttcataca tctctccgat cattttgcaa gtttcacgta
    33361 gcacaatgcc gttttactgc atcaaatgaa caactaaaag catcattttg tggttgcatt
    33421 tttgtagctt tatgaatggc aggaaatcat taaaagtctg tcctcaccat caatgcagtt
    33481 agttaagatc ctgattggtt gtaaacgcaa caaaagttta ggcccctgag atgaaagaga
    33541 aaaacaaacg tgccacaaga tacaaaaaat cccgaccagt agtcaggatt tttttgcatt
    33601 tcaccttaac gttggtcgct ttgacttgaa gaggtaactt tgaacctaac aatgagagat
    33661 aactgttatt acagattgtg cgaaaatata atcagcagtg atttattcct acctacactt
    33721 cgcagctaac acctcatcgt tttcaagttg ctctttgtgt agataatccc cagcaacagg
    33781 tggaggcgtt accagtatcg ggcgacctgt tttaggatca tgtaccgtaa ccaacccaac
    33841 aatctcccct tcatgggtta gtgcccatgc agctaattga taaacagtgc tcttggttgg
    33901 gctaccctca acattatcat gacgaaaata ccaatccggt gatggaatta tctgtttgta
    33961 gagtgccgga ggcctattgt ttggattgtt agcagttgat ttatcctcta gcgcctcctt
    34021 tagaagcaac cgtgcttgcc cttctataga gcgctctgac attgccgcca tttcttcaag
    34081 gcggcggtaa agatcatcag ggaagtttct aacttgtatt tttgccataa cgcatgactc
    34141 acattgagat ctaaatgagt catcatgtta cttgatgttg caagtaaagt gaacactaat
    34201 acgctgaggc ccgattagta acaacagtga tgatgcggtc aattctggat tattttttta
    34261 tggcgagtcc gtccagaatt gttcgtcttt tagtccagaa ttgttcgccg cgttac
//
LOCUS       DAZPEP010000027        34277 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZPEP010000027 DAZPEP010000000
VERSION     DAZPEP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34277
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.027"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..191)
                     /locus_tag="ACHUT9_001309"
     CDS             complement(<1..191)
                     /locus_tag="ACHUT9_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347830.1"
                     /translation="MIGHITNSGGNNSLMKHDVVQGNNIVDLDLLRNFNGVTGLNRDN
                     FIYISDIFLNIKQRNEKIM"
     gene            complement(420..1340)
                     /gene="malM"
                     /locus_tag="ACHUT9_001310"
     CDS             complement(420..1340)
                     /gene="malM"
                     /locus_tag="ACHUT9_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFR1347831.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKSDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1492..2832)
                     /gene="lamB"
                     /locus_tag="ACHUT9_001311"
     CDS             complement(1492..2832)
                     /gene="lamB"
                     /locus_tag="ACHUT9_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFR1347832.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2904..4019)
                     /gene="malK"
                     /locus_tag="ACHUT9_001312"
     CDS             complement(2904..4019)
                     /gene="malK"
                     /locus_tag="ACHUT9_001312"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFR1347833.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4384..5574
                     /gene="malE"
                     /locus_tag="ACHUT9_001313"
     CDS             4384..5574
                     /gene="malE"
                     /locus_tag="ACHUT9_001313"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFR1347834.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5728..7271
                     /gene="malF"
                     /locus_tag="ACHUT9_001314"
                     /pseudo
     CDS             5728..7271
                     /gene="malF"
                     /locus_tag="ACHUT9_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
     gene            7286..8176
                     /gene="malG"
                     /locus_tag="ACHUT9_001315"
     CDS             7286..8176
                     /gene="malG"
                     /locus_tag="ACHUT9_001315"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFR1347835.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8548..10023
                     /gene="xylE"
                     /locus_tag="ACHUT9_001316"
     CDS             8548..10023
                     /gene="xylE"
                     /locus_tag="ACHUT9_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFR1347836.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10067..10477)
                     /gene="psiE"
                     /locus_tag="ACHUT9_001317"
     CDS             complement(10067..10477)
                     /gene="psiE"
                     /locus_tag="ACHUT9_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFR1347837.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(10603..10746)
                     /locus_tag="ACHUT9_001318"
     CDS             complement(10603..10746)
                     /locus_tag="ACHUT9_001318"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347838.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            10747..11025
                     /locus_tag="ACHUT9_001319"
     CDS             10747..11025
                     /locus_tag="ACHUT9_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347839.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(11072..13168)
                     /locus_tag="ACHUT9_001320"
     CDS             complement(11072..13168)
                     /locus_tag="ACHUT9_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1347840.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13168..13905)
                     /locus_tag="ACHUT9_001321"
     CDS             complement(13168..13905)
                     /locus_tag="ACHUT9_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1347841.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13902..14540)
                     /locus_tag="ACHUT9_001322"
     CDS             complement(13902..14540)
                     /locus_tag="ACHUT9_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFR1347842.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14654..14896)
                     /gene="yjbE"
                     /locus_tag="ACHUT9_001323"
     CDS             complement(14654..14896)
                     /gene="yjbE"
                     /locus_tag="ACHUT9_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFR1347843.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15358..17007)
                     /gene="pgi"
                     /locus_tag="ACHUT9_001324"
     CDS             complement(15358..17007)
                     /gene="pgi"
                     /locus_tag="ACHUT9_001324"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFR1347844.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      17244..17440
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17532..18881
                     /gene="lysC"
                     /locus_tag="ACHUT9_001325"
     CDS             17532..18881
                     /gene="lysC"
                     /locus_tag="ACHUT9_001325"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFR1347845.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            19133..19405
                     /locus_tag="ACHUT9_001326"
     CDS             19133..19405
                     /locus_tag="ACHUT9_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFR1347846.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(19538..20410)
                     /gene="rluF"
                     /locus_tag="ACHUT9_001327"
     CDS             complement(19538..20410)
                     /gene="rluF"
                     /locus_tag="ACHUT9_001327"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFR1347847.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            20758..21595
                     /locus_tag="ACHUT9_001328"
                     /pseudo
     CDS             20758..21595
                     /locus_tag="ACHUT9_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            21665..22471
                     /locus_tag="ACHUT9_001329"
     CDS             21665..22471
                     /locus_tag="ACHUT9_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1347848.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            22481..22888
                     /locus_tag="ACHUT9_001330"
     CDS             22481..22888
                     /locus_tag="ACHUT9_001330"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFR1347849.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            22888..23382
                     /locus_tag="ACHUT9_001331"
                     /pseudo
     CDS             22888..23382
                     /locus_tag="ACHUT9_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            23448..24245
                     /locus_tag="ACHUT9_001332"
     CDS             23448..24245
                     /locus_tag="ACHUT9_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFR1347850.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            24256..25080
                     /locus_tag="ACHUT9_001333"
     CDS             24256..25080
                     /locus_tag="ACHUT9_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFR1347851.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            25132..26360
                     /gene="sorE"
                     /locus_tag="ACHUT9_001334"
                     /pseudo
     CDS             25132..26360
                     /gene="sorE"
                     /locus_tag="ACHUT9_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            26743..27432
                     /gene="pepE"
                     /locus_tag="ACHUT9_001335"
     CDS             26743..27432
                     /gene="pepE"
                     /locus_tag="ACHUT9_001335"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFR1347852.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(27523..29154)
                     /locus_tag="ACHUT9_001336"
     CDS             complement(27523..29154)
                     /locus_tag="ACHUT9_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFR1347853.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(29374..33057)
                     /gene="metH"
                     /locus_tag="ACHUT9_001337"
     CDS             complement(29374..33057)
                     /gene="metH"
                     /locus_tag="ACHUT9_001337"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFR1347854.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            33257..34081
                     /gene="iclR"
                     /locus_tag="ACHUT9_001338"
     CDS             33257..34081
                     /gene="iclR"
                     /locus_tag="ACHUT9_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFR1347855.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         8065 a         8955 c         8863 g         8394 t
ORIGIN      
        1 tgcatgattt tttcgttccg ttgttttata tttaaaaata tatcactgat ataaataaag
       61 ttatctctgt ttaaacctgt caccccattg aaattacgta gtaaatcaag atctactata
      121 ttatttcctt gaaccacatc atgtttcatc aaagagttat tgccgccaga atttgtgata
      181 tgacctatca taaaacctct gtaatacaga aataaaataa cctggaaaag tagcctgagc
      241 gtccagacac taccggcgaa aggaaaagaa tctctgataa ggcattgaga taatggacat
      301 tcttagtgaa gaagaagtga agtactataa aaaatcgctc atatcccagg aaggaagcat
      361 aaaaaaaggt gcgccaggag acgcaccagt tgttgcaaaa tcagcactgt ggggcgtaat
      421 taccccttgc cttttacact gctgataaag gtggaacggg cagatgtcga tcccaggcgt
      481 tcagcttcat caagcagttt taacgcctta tcaacatcac ttttcgcgac agcgtttttg
      541 atcgcggtat taaaataact ttccgtgtcg ttgagcatcg gctcgctttt cttcaccggc
      601 accggagcag gtgcagccac agctggtgcc gccgtgttgc ctaccgtaac cggagctgga
      661 gcggaagaac caaatagtgg tcctaccaac acgctggagc tggagttcgt tttgactttc
      721 agtttcagta agccatcgct ggtatgacga gcaaccggat cgggaatatc cgggatcgag
      781 ttaccgacgc ctttggcata ggctttagcc gggtcgagca gttgggtcgt ctgctggaga
      841 tctttttccg tggtaaagac cagaacataa agtttttgct gccccaacgc cggtgtcagg
      901 cgcataacgc cttccagccg atctgcactc atcacgcctg gttcctggta ggtgaaataa
      961 ctgctgggga agaaggctga tggggtcatg ttctgatcaa gaatcagcac gttcggcgca
     1021 aaaacgctgg tttgtttgtt cacttcgctg gtcagcgtca gggtcagttc gccaatgttt
     1081 gccgggacgc tgtacgcagc aaccggacca ctgatgccgg gaacgttcag ttgttggccg
     1141 ccggtcgcca gttgggtggt ctgggtttta gattgatcga ccggtgtcca ggtgagttgt
     1201 tgcagcgcgg cagatggaat ggctggcgcg tcgctggtgt tttgcggtac gtagttaaca
     1261 tcggcaaggc taattccagg cgcgcttgcc agtaaccctg ctgataaaca gagggcgatg
     1321 agacttttat tcattttcat tgttttcacc tcaaaatctg gagctcagcg gtagccaggc
     1381 aatagcgcgc taaacccgat aatcagaggg gctttcgccc ctttcagata atgacaacct
     1441 gtttttatgc cggatgcggc gtaaacgcct tatccggccc aggttttgct attaccacca
     1501 gatttccatc tgggcaccga aggtccactc gtcgctgtcg ccacgaccga agctgccgcc
     1561 gttgaaatca gcaggaacgg ctttgccgta gttcgggtta accttgctat cgccgttgta
     1621 gtcgtaaccc catttctcat cccacttggc gtaggttgcg aagacacgaa tagccgggcg
     1681 tgaccagatg ctgtcgccag cctgccattg ttgtgcgagg gtaattttgt actgattgtt
     1741 cttgtcgccg gtgcgctggg attcgacgtt gtcgtagccg atttccatca cggtgctcat
     1801 gattggcgtc cacttgtaca tcgggcgaat accgacggtc caccacttgg tgccgttgtc
     1861 gttatcccag ttgatatcct ggtacatacc cacgtacatc atgtcccagt tgtcgcccat
     1921 ggagatcgca ccgtggtcga ggatacgcag catgtgaccg ttgttgttga tattgtaggc
     1981 aaatttttcg ttatcaaacg cgacgccaga accctgcgac agacctttac cctgcgaggt
     2041 catcgagtca gtagcgtact gaacaacaaa cttgttaaag cccttcagga cactctgagt
     2101 atgttcagca gtgaataacc agccgtcttt cgatgcgcca tcaaccagac gatagttatc
     2161 acgcaggttg gcacgaccgt agtcgacacc cagttctaat gtgccgcccg ggttgatttc
     2221 catctgcgct aaacgcacat cgaaaacgtc gttcgcggtt tcgttggtat agtcataaat
     2281 attgttgctg gcaaaagagg aagaaccacc agcttcagag gagcgggttg ctgccagaga
     2341 gagtttaccg aagccaacat cgatgttttc cagaccggca ccaggaccag aaatatccca
     2401 gtagtagaag tcgatcatat gaacgtcatg acgttggtag aagcgcttac ctgcccagat
     2461 ggtggaacct ggcagccatt cgatcaggtt tttaccctgc acgtttgctt cacggaaggc
     2521 cggatcggta gcttcccagt cattctgttg cgcgacggaa taggccacgt tagtgtcgaa
     2581 atagaagctc ttatcgccct ctttccacac ttcctgaccc aattttaatt cagcataagt
     2641 ttcacattcg ttgccaagac ggtatttact ttgagcaccg gtagtctgga aacactgttg
     2701 ttcaccgccg ctacctgtcc agccaatacc ggaacgtgca tagccgtgga aatcaacagc
     2761 cattgcctga gcagacatta cgcccgctgc gacggcaacc gccagaggaa gtttgcgcag
     2821 agtaatcatc attctatctc ctgagtcatt gcttttcttt tttcacatca cctgtgacag
     2881 gctttgtgtg ttttgtgggg tgcttaaacg cccggctcct tatgcagtcg acgacatgca
     2941 gtgccatcct cacggaacag atggcaacgc tctggcggca ggccgatagc gaatgtggca
     3001 ccttcttcta ccaacaccac gtcgttctgg cggtacacca ggttttgacg aatggaaggg
     3061 atctggatat ggatttgggt ttcgttgccg agttgctcga cgacctgaac ttcaccctca
     3121 aggatgacgt cagcgatatc actcggcagt agatgttccg ggcgaatacc cagcgacata
     3181 ttggctccaa cctggacatc acggctttca actggcagcc agacttgctg acgatttggc
     3241 atcggcagct ccacctgcac ttgatcgatt gcggtggcgg tcacttttac cggcaggaag
     3301 ttcatctttg gcgaaccgat aaatccggcg acaaaacggt ctgccggata gtggtacagc
     3361 tccagcggtt tcccaacctg cgccacgcga ccggcgtcca gcaccacgat tttgtcggcc
     3421 agcgtcatcg cttcgacctg atcgtgggtg acgtaaatca ttgtgcggcc caggcgttta
     3481 tgcagacggg agatttcgat acgcatttgc acacgcagtg cagcatcgag gttggagagc
     3541 ggttcatcga gcaaaaatac gcttggctcg gccaccagcg tacggccaat cgccacacgc
     3601 tgacgctgac caccggagag cgctttcggt ttgcgatcca gcaaatgcgc cagttgcagc
     3661 acttccgcca cctggttgac gcgttggtta atcacctctt tttttgcgcc agccagtttc
     3721 aggccaaatg acatgttttc tgctactgac aggtggggat agagcgcgta agactgaaac
     3781 accataccaa cgccgcgttc tgctggcgga gtgtcattca tccgtttctc accgatgaac
     3841 aggtcgccgc tggtgatcgt ctcaagcccg gcaatcatgc gcagtaaagt cgatttaccg
     3901 cagccagacg gtccgacaaa caccacgaat tcaccttcat ggatatcgag attgatatct
     3961 ttcgatacca cgacctcgcc ccaggctttc gttacatttt gcagctgtac gctcgccatg
     4021 cccttctccc tttgtaacaa cctgtcatcg acagcaacat tcatgatggg ctgactatgc
     4081 gtcatcagga gatggcttaa atcctccacc ccctggcttt tttatggggg aggaggcggg
     4141 aggatgagaa cacggcttct gtgaactaaa ccgaggtcat gtaaggaatt tcgtgatgtt
     4201 gcttgcaaaa atcgtggcga ttttatgtgc gcatctccac attaccgcca attctgtaac
     4261 agagatcaca caaagcaacg gtggggcgta ggggcaagga ggatggaaag aggttgccgt
     4321 ataaagaaac tagagtccgt ttaggtgttt tcacgagcac ttcaccaaca aggaccatag
     4381 attatgaaaa taaaaacagg tgcacgcatc ctcgcattat ccgcattaac gacgatgatg
     4441 ttttccgcct cggctctcgc caaaatcgaa gaaggtaaac tggtaatctg gattaacggc
     4501 gataaaggct ataacggtct cgctgaagtc ggtaagaaat tcgagaaaga taccggaatt
     4561 aaagtcaccg ttgagcatcc ggataaactg gaagagaaat tccctcaggt tgcggcaact
     4621 ggcgatggcc ctgacattat cttctgggcg cacgaccgtt ttggtggcta cgctcaatct
     4681 ggcctgttgg ctgaaatcac cccggacaaa gcgttccagg acaagctgta tccgtttacc
     4741 tgggatgccg tacgttacaa cggcaagctg attgcttacc cgatcgctgt tgaagcgtta
     4801 tcgctgattt ataacaaaga tctgctgccg aacccaccaa aaacctggga agagatcccg
     4861 gcgctggata aagaactgaa agcgaaaggt aagagcgcgc tgatgttcaa cctgcaagaa
     4921 ccgtacttca cctggccgct gattgctgct gacgggggtt atgcgttcaa gtatgaaaac
     4981 ggcaagtacg atattaaaga cgtggccgtg gataacgctg gcgcgaaagc gggtctgacc
     5041 ttcctggttg accttattaa aaacaaacac atgaatgcag acaccgatta ctccatcgca
     5101 gaagctgcct ttaataaagg cgaaacagcg atgaccatca acggcccgtg ggcatggtcc
     5161 aacatcgaca ccagcaaagt gaattatggt gtaacggtac tgccgacctt caagggtcaa
     5221 ccatctaaac cgttcgttgg cgtgctgagc gccggtatta acgccgccag tccgaacaaa
     5281 gagctggcga aagagttcct cgaaaactat ctgctgactg acgaaggtct ggaagcggtc
     5341 aataaagaca aaccgctggg tgcagtggcg ctgaagtctt acgaggaaga gttggcgaaa
     5401 gatccacgta ttgccgccac catggaaaac gcccagaaag gtgaaatcat gccgaacatc
     5461 ccgcagatgt ccgctttctg gtatgccgtg cgtactgcgg tgatcaacgc cgccagcggt
     5521 cgtcagactg tcgatgaagc cctgaaagac gcgcagactc gtatcaccaa gtaatgctgt
     5581 gaaatgccgg atgcggcgtg aacgccttgt ccggcctaca aaactgaaac gcatgtaggc
     5641 ctgataagac gcatcagtgt cgcatcaggc agttgttgtc ggataaggcg tgaaagcctt
     5701 atccgtcctg gaatgaggaa gaaccccatg gatgtcatta aaaagaaaca ttggtggcaa
     5761 agcgacgcgc tgaaatggtc agtgctaggt ctgctcggcc tgctggtggg ttaccttgtt
     5821 gttttaatgt acgcacaagg ggaatacctg ttcgccatta ccacgctgat attgagttca
     5881 gcggggctgt atattttcgc caatcgtaaa gcctacgcct ggcgctatgt ttacccggga
     5941 atggctggaa tgggattatt cgtcctcttc cctctggtct gcaccatcgc cattgccttc
     6001 accaactaca gcagcactaa ccagctgact tttgaatgtg cgcaggaagt gttgttagat
     6061 cgctcctggc aagcaggcaa aacctataac tttggtcttt acccggcggg cgatgagtgg
     6121 caactggcgc tcagtgacgg cgaaatcggc aaaaattacc tctccgacgc gtttaaattt
     6181 ggcggcgaac aaaaactgca actgaaagaa acgaccgccc agcccgaagg cgaacgcgcg
     6241 aatctacgcg tgattaccca gaatcgtcag gcgctgagtg acattaccgc cattctgccg
     6301 gatggcaaca aagtgatgat gagctccctg cgccagtttt ctggcacgca gccgctctac
     6361 acactcgacg gtgacggcac gttgacgaat aatcagagcg gcgtgaaata tcgtccgaat
     6421 aaccaaattg gcttttacca gtccattacc gccgacggca actggggtga tgaaaagcta
     6481 agccccggtt acaccgtgac tactggctgg aaaaacttta cccgcgtctt caccgacgaa
     6541 ggcattcaga aaccgttcct cgccattttc gtctggaccg tggtgttctc gctgatcact
     6601 gtctttttaa cggtggcggt cggcatggtt ctggcatgtc tggtgcagtg ggaagcattg
     6661 cgcggcaaag cggtctatcg cgttctgctg attctgccct acgcggtgcc atcattcatt
     6721 tcaatcttga ttttcaaagg gttgtttaac cagagcttcg gtgaaatcaa catgatgttg
     6781 agcgcgctgt ttggcgtgaa gcccgcctgg ttcagcgatc cgaccaccgc ccgcacgatg
     6841 ctaattatcg tcaatacctg gctgggttat ccgtacatga tgatcctctg catgggcttg
     6901 ctgaaagcga ttccggacga tttgtatgaa gcctcagcaa tggatggcgc aggtccgttc
     6961 cagaacttct ttaagattac gctgccgctg ctgattaaac cgctgacgcc gctgatgatc
     7021 gccagcttcg cctttaactt taacaacttc gtgctgattc aactgttaac caacggcggc
     7081 ccggatcgtc ttggcacgac cacgccagcc ggttataccg acctgcttgt taactacacc
     7141 taccgtatcg cttttgaagg cggcgggggt caggacttcg gtctggctgc agcaattgcc
     7201 cgctgctctt cctgctggtg ggtgcgctgg cgatagtgaa cctgaaagcc acgcgaatga
     7261 agtttgatta agggagataa caaaaatggc aatggtccaa ccgaaatcgc aaaaagcacg
     7321 tttgtttatt actcacctgc tactgctact ttttatcgcg gcgattatgt tcccgctgct
     7381 gatggtcgtc gctatctcac tgcgccaggg gaactttgcg atcggcagcc tgatcccgga
     7441 gcaaatctcc tgggatcact ggaaactggc tttaggtttt agcgtggaac aggctgatgg
     7501 tcgcattacg ccaccgccat tcccggtact gctgtggcta tggaactcgg taaaggtcgc
     7561 cgggatttcc gcgattggca ttgtcgcgct ctctaccacc tgcgcctacg ctttcgcccg
     7621 tatgcgcttt ccaggcaaag cgacgctgct gaaaggaatg ctgattttcc agatgttccc
     7681 ggcagtactt tcactggtcg cgttgtatgc gttgtttgat cgtctgggtg agtacattcc
     7741 attcattggc ctgaatactc acggcggcgt aattttcgcg tatctgggtg ggattgcgct
     7801 gcatgtctgg accatcaaag gctatttcga aaccatcgat agttcgctgg aagaagcggc
     7861 tgcgctggat ggtgcgacac cgtggcaggc cttccgcctt gtcctgttgc cgctgtcagt
     7921 accgattctg gcggtggtat tcatcctgtc gtttatcgct gccattactg aagttccggt
     7981 cgcgtcgctg ttactgcgtg acgtaaacag ctacaccctg gccgtgggga tgcagcaata
     8041 cctcaacccg caaaactacc tgtggggtga ctttgccgcc gctgccgtga tgtctgcatt
     8101 accgatcacc atcgtcttct tgctggctca acgctggctg gtcaacggcc tgacggcagg
     8161 tggtgtgaaa ggttaaagat gttgttctgc caatgttatg ccgctgcacc ctcaacttac
     8221 gttatcccaa cttgtgactg ttattcggcg ctccacggag cgcctttttt tctttcgtct
     8281 gcaatctgaa tcgttcgccg gttaatattt ccatcataga gcttattatt tttacgttat
     8341 ttgttttccc acttacgata attctctttc gtgctctgag tcacggcaat agtattgttt
     8401 tcatcaattt ttgataatta tcacaattaa gatcacagaa aagacattac gtaaacgcat
     8461 tgtaaaaaat gataattgcc ttaactgcct gacaattcca acatcaatgc actgataaaa
     8521 gatcagaatg gtctaaggca ggtctgaatg aatacccagt ataattccag ttatatattt
     8581 tcgattacct tagtcgctac attaggtggt ttattatttg gctacgacac cgccgttatt
     8641 tccggtactg ttgagtcact caataccgtc tttgttgctc cacaaaactt aagtgaatcc
     8701 gctgccaact ccctgttagg attttgcgta gccagcgctc tgattggttg catcatcggc
     8761 ggtgccctcg gtggttattg cagtaaccgc ttcggccgtc gtgattcact taagattgct
     8821 gctgtcctgt tttttatttc tggtgtaggt tctgcctggc cagaacttgg ttttacctct
     8881 ataaacccgg acaacacagt acctatttat ctggcaggtt atgtcccgga atttgttatt
     8941 tatcgtatta ttggcggtat tggcgttggt ttagcctcaa tgctttcacc aatgtatatt
     9001 gcggaactgg ctccagctca tattcgcgga aaactggttt catttaacca gtttgcgatt
     9061 attttcgggc aacttttagt ttactgcgta aactatttta ttgcccgttc cggtgatgcc
     9121 agctggctga atactgacgg ctggcgttat atgtttgcct cggaatgtat ccctgcactg
     9181 ctgttcttaa tgctgctgta taccgtgcca gaaagtcctc gctggctgat gtcacgcggc
     9241 aagcaagaac aggcggaaag tatcctgcgc aaaattatgg gcaacacgct tgcaactcag
     9301 gcagtacagg aaattaaaca ctccctggat catggccgca aaaccggtgg tcgtctgctg
     9361 atgtttggcg tgggcgtgat tgtcatcggc gttatgctct ccatcttcca gcaatttgtc
     9421 ggcatcaatg tggtgctgta ctacgcgccg gaagtgttca aaacgctggg ggccagcacg
     9481 gatatcgcgc tgttgcagac cattattgtc ggagttatca acctcacctt caccgttctg
     9541 gcaattatga cggtggataa atttggtcgt aagccactgc aaattatcgg cgcactcgga
     9601 atggcaatcg gtatgtttag cctcggtacc gcgttttaca ctcaggcacc gggtattgtg
     9661 gcgctactgt cgatgctgtt ctatgttgct gcctttgcca tgtcctgggg tccggtatgc
     9721 tgggtactgc tgtcggaaat cttcccgaat gctattcgtg gtaaagcgct ggcaatcgcg
     9781 gtggcggccc agtggctggc gaactacttc gtctcctgga ccttcccgat gatggacaaa
     9841 aactcctggc tggtggccca tttccacaac ggtttctcct actggattta cgggtgtatg
     9901 ggcgttctgg cagcactatt tatgtggaaa tttgtcccgg aaaccaaagg taaaaccctt
     9961 gaggagctgg aagccctctg ggaaccggaa acgaagaaaa cacaacaaac tgctacgctg
    10021 taatcttcct gtccagcacg ccgcgccagt tcggcgtgct ttctttttac tcccgcttca
    10081 gccgtttcga attacacagc cacagagtga tcaccagtaa caggatcgca gccgagtaga
    10141 tcaacacatc cagtggcgat ttatgatcga cgatgatcaa gcgcacaatg gcggtgatcc
    10201 caatatagac aaagtaacgt agcgggaagt gaaaaccgga ctgaaagtac ttcacaatca
    10261 gcgcgataaa ttcgaaatag agaaagtaaa ccaccagtcc ttctaccagc tcatatttgc
    10321 tggtttgttc tggcgcgaac agcacatctg ccagatgcac cgtttctttg ccgaggaaga
    10381 caaccaaaat caggccgagg cacagcaggc caagattgag tacggtctgc aaaatggtgg
    10441 agataaactc cacgcgcgga cgagagagtg acgtcatagc agtacctcct tcacagtggc
    10501 gagcttcctg tataacgcaa aaatgtgacg gagatctata ttttttgtgt atgttttgtt
    10561 caacctggat ttggagcgcg cctccaccaa agccggatat acctacccgt aggccggata
    10621 agccgcgcct ggcggcgcat ccggcaagac aaaccgcacc caagccggta attcaaaaat
    10681 cacccattca ggtgattccc agccaccagc aatgcgcgaa aaatcgacaa tgttcctctg
    10741 gaacaaatga aacgtaacct gataaaggtg gtaaaaatga agccttattt tgctgctttg
    10801 atgttatcag tctctgttct tcctgcttat gctggtccgc tcggtactgc tgataaagcc
    10861 gacctaccgc aaagcaatgt ctccagccca atgatggccc agtcgctcag gcaaccagac
    10921 ctgcaaccga tatcgacgga caggaagaca gaatgttttc gcctttacac accagatcgc
    10981 aaaccggggg tgaactgcgt tcctgatggc tcaacaggcc attaagaatg agggtgccag
    11041 tgattgcgaa aactggcacc tttcccatga cttagcggaa attgacgcta cggtcgctgg
    11101 tcatatcata caactggaac ttacgcccaa gttgctgacc accgtcacgc gtcagcggcg
    11161 tccagccaat cgccgcacgg ctgcgtgtcg ggccagacga gaagagatcc aacggtaccg
    11221 agacatacac gcctttggtg aagtcccctt cgccgtactc ctcttttgaa acattcgtga
    11281 tcgtggcata gccacccacc accacgccgc tatcaaagcg tttggcgatc tccagcgtgc
    11341 cgcctttatc ccctgccaga tactgcccga cgctggcttt cactaacacg tcctgggcga
    11401 aagatggcgt ccagtaggcg gtcagatgtc cggttttcac gctgtagtcg gtgaatttca
    11461 tcatatcttt tgcactacgc cagtcgcgct gtttaacgta gttggcatcc agaccaaacg
    11521 cccagttgct gtccagcgga cgatacaaca cttcagcccc cgcaccgcca aacatggttt
    11581 cgagataacc tccatagacc tgaccgtaga agccgttgcc cagatgctgg aagtagttgg
    11641 cttgcaggtt attcacatag gcatcgttct gcacatactc gcgcacatgg gtacgaacgc
    11701 gcggtaagtg cgagtcctgt ggcggattgg tgtagttaaa cttgtcgtag ttgttggcaa
    11761 gatttgcaaa caggctgccg atggtcagca gatggtccgt cagccacaaa tctgccgttc
    11821 ccatcacgcc cagctgatac atgtaaaagt tttccgggcc accgaccgac tggttcagca
    11881 ccggatcgat atggaaatca aagcgtgatt tgtcgatata ccagccctgc tcggtggact
    11941 gcggaaccac tggctcgacg cgtttttgcg ccagcgtcgt ttcgtggccc aacggctctc
    12001 cggcgagatg atttttcagg ctggcgacat cggtttccgt cgtcacctgc ggcatgttaa
    12061 ggcgattttc cgtaatgcgg atcgttttga tcccatccgg cagatcgttc atcacgatcc
    12121 gattagcacg gatgatccct tcgcgcgaat cacgatattt cacctgctcg ccggtaacat
    12181 acagcgtatc gcctttcgcc tggatctgcg gatcggcaag tccggcattg tatttcagca
    12241 acgttaactg attcgccacc accgagtgct gcaaaatggc atcctgcggc tgcggttgat
    12301 attgcgggcg ggcgttatcg atgtaggacg ggcgcagatc gttaaagttg gtgcgcagcg
    12361 tgacgccaaa cataaaggtg ttgccacgtt catagctaag gttaacgtcg gcccaatcgg
    12421 taacgcgata aatcgccccg acgttaaact tacttttttg ctccagcttc ccggcaaaat
    12481 cctgctgata attattacct tcatactcca gtttcaggcg cagcggttgc cagggcgtct
    12541 ggtattccac gccgccaaac agtgaggccg ggccgtggaa catctgactg ccgtcgatag
    12601 atcccgcttg tttgtagctg ttatcgcgat agcaatattt atcactggct gaacagagcg
    12661 gatttttcac attaccgctg gtgcccagat atccccagcc caggccgagc gtaaaatcga
    12721 acggccccca ggctttgctg gcaacaagat attccgcatc aaacagcccc gtaccgccga
    12781 tatcccgcgc accaaccgcc acttgcggca gccagtaact ctcttcccac aaacgcagtt
    12841 tgagatcgaa ggctttatct ttatacgttt gatcgccaga gaacgcttcg acgctgctgt
    12901 actgccgggt gcgcacgtcg gtgtaacgca gcgttgtttc cagccacggg aagagttgca
    12961 ctgaagctga gtaataacgg tactgatcgt tatcgcgata gttcagactc aactcccctt
    13021 cccgtgccat acgcgcggtg ggagtttgta ataatcctac gccaccgaaa tccgactgcg
    13081 acggaccaat gggcgcagga tatgtttcgc cgtagcaagc tgtgctaatg cccagcgcca
    13141 gtaagctaag cagatgtctt tttttcatta ttggggtatc cgctgcgtca gagtctgaag
    13201 aatgtcggcg ttaagggcat caggcgtctc actccagacg gagtctgcga ggccaacata
    13261 aataatgctg ccgggcatcg gctctacgtg acgcttgttc cagtaagcca ccggcgcttt
    13321 ttgcgtgcgt ccgtccgggt aaacgaccca cgcgtagctg cgatccgcac cgctgagcag
    13381 gctctggtca gagagatagc tcgccacgtc gcgacctgga gtgaatggct gattgccagg
    13441 atggctgata agcccgaaca acgtgaccgt ggacggcggt ggtccgaccc acagcgtgta
    13501 gttgccctgc aacggcgggt taccgcgttc ggcaacgcgg acgatatcgg gatcaagatt
    13561 gattttttgc ctgcctgtca cttccaatgc ctggatttgc tggcgtaagg cgttaatggc
    13621 ggcggcatcg tcggtgctgg aatctgcgcc ctgttctgcc agccgtgtca gcaacgcctg
    13681 ctgctgacgc aatgctgccg ccgttgccag ctcttcgcta atcaccgcac caggccacca
    13741 gctgtttgcc agtcgaggtt gccctaccag atcgattaaa tgctctgcgc ccgttagcgt
    13801 tttggcctca ctgctgccat tgctgaacac cttgacggtt cctgccgcga agaccgatga
    13861 cgctcccaca ctcaaaagca acgcgacaat agtttgttta atcattgttt tgccgccttg
    13921 atgagagtgg ttttcaccgg gaaataatcc gcgccgagat actgttccga ctggcgaatt
    13981 tgcccttcgc tgtcgatcca gtagcggtta tgccagcgcg cctggtcggt ggagacttct
    14041 tcgtccagca cgcgaaccag ggtttcatcg ctgccgacct tgacggtatc ggtgccatcc
    14101 catttgaaga ctgagcgtgc ggtggcgtag cgtacctgct ggtactcggt ccagcccatc
    14161 gtgcgcgtcc atgttgcacc atcaacaatt tgtgcgggtt taatcagcgg gtcggcggca
    14221 aggttattca cttcaatcag gttgtcgccg ccaagcaaag tcttcaccag acgaccatgt
    14281 tgtgtgacaa gagtggcttg atcctgagtg acccattttt gttgtccgtc ttcagcgaag
    14341 gccagcacca caaacaattg cggcccgcca ttaagctgca tgtactggct ggcgtagggc
    14401 atattttgaa tatcatcgtc cgtcagctgt acgcctggcg tgccgaacag actgtcccac
    14461 agtgaattgc ccagctcttt agtcgtggct gaacaggcct gtaataacag gcagataaga
    14521 atgagtgcag gtcgcttcac gactcttctc cagaggggca gaataaccac accggagtgt
    14581 ggttatggtt aatcccttca tatttcaagc cacagatgca atgactcgaa ttattttggg
    14641 gatacatact ttattactgg gtactggtgg ttgtggttgt tgtggtcccg gtattagaac
    14701 catcaccgcc accggttgcc gcgagcgcga cacccaccgc cgagcttacg gtgctgacgc
    14761 tggtcgcgga ggaactcccc gccgaaaccg acgttgctgc cgcccctgcc gcttcgccca
    14821 cctgtacagg tgcagcccac acagaagtcg ccgcaagcgc agatatggca aaaatgccat
    14881 acagaacttt tttcataaca atttcccttc attgaatgaa tggagattta cccaaaaaca
    14941 tttcgggcgg gatcgagtat acacaactaa agcatgcgaa ttacggcgcg gggaattagc
    15001 gatggtgttt taggataatt ggaatgaata tcaaatataa gtttcaactt atatttaaat
    15061 aacaataaaa atcataaact taaaataaaa acaaaagtaa ttaatctgcg tattctccga
    15121 aataaacaac gattaaaaat ctattttagg attaacctca cggtatgatt tccgttaaat
    15181 cacagacaag gggatttatc tgataaaaca atgccgactt attgtcggca ttgtttttaa
    15241 ggttatttaa ctcctcatac tatgcattaa tgtcgatatt ggcatcaggc tccggttgcc
    15301 ggatgcggcg taaacgcctt atccggccta catatcggca tcaggcacgc atgatgatta
    15361 accgcgccac gctttatagc ggttaatcag accattggtc gagctatcgt ggctgctgat
    15421 ttctttgtca tctttcagct ctggcagaat acggttcgcc agttgtttac ccaactccac
    15481 gccccactgg tcgaaggtaa agatgttcag gatcacgccc tgagtaaaga ttttgtgctc
    15541 atacagcgca atcaacgcac ccaggctgaa cggagtgatt tcgcgcagca ggatggagtt
    15601 ggtcgggcgg ttaccttcga acactttgaa cggcaccacg tagtcaagcg ttgccggatc
    15661 tttaccctga tcgcgatatt cctgctcaac cacttcgcgg gatttaccaa acgccagcgc
    15721 ttcggtctgg gcgaagaagt tagacagcag tttctggtgg tgatcagaga gcgggttatg
    15781 ggtgatagcc ggggcgatga aatcgcacgg taccattttg gttccctggt ggatcagctg
    15841 gtagaacgcg tgctgaccgt tagtgcctgg ttcaccccag ataatcgggc cagtctggta
    15901 atccacaacg ttaccgttac ggtcaacata cttaccgttg gactccatat tgccctgctg
    15961 gaagtacgcc gcgaaacggt gcatatactg gtcatacggc agaatcgctt cagtttccgc
    16021 accaaagaaa ttgttgtacc agatgccaat cagcgccagc aggacaggca ggtttttctc
    16081 ggcaggcgtg gtggagaaat gcttgtccat cgcgtgtgcg ccagaaagca gttcaacgaa
    16141 gttatcaaag ccgatggaga gaacaatcga caggccaatc gctgaccaca aagagtaacg
    16201 gccgccgacc cagtcccaga actcgaacat gttggcagta tcaataccaa actcgccaac
    16261 ggctttggca ttggtggaaa gcgccgcaaa gtgttttgca acgtgttttt catcacctgc
    16321 cgctttcagg aaccagtcac gcgcgctatg ggcgttggtc atagtttcct gagtggtgaa
    16381 ggttttagat gctaccagga acagcgtggt ttccgggttt acttttttca gcacttcagc
    16441 gatgtgagtc ccatcgacgt tagaaacaaa gtgcatgttc aggtgatttt tgtacggacg
    16501 cagtgcttcg gtcaccatgt acgggccgag gtcagaacca ccgatcccga tgttcactac
    16561 gtcagtgatt gctttgccgg tataaccctt ccactcaccg gagataatcg cttccgagaa
    16621 ggttttcatc ttctccagca ccgcgttgac ttccggcatt acgtctttgc catcaaccag
    16681 aatcggggta ttgctacggt tacgcagcgc tacgtgcagc acggcgcggt tttcagtgcg
    16741 gttgatcttc tcgccagaga acatcgactt aatcgcgccc gccagatcgc actctttcgc
    16801 cagatcctgt aatttcgcca gcgtctcttc agtgatgcgg tttttggagt aatccaccag
    16861 catctgatcg tcgaatgttg cggagaactt agaaaaacga tcgccgtctt tagcaaaaag
    16921 atcggcgatc gtaacgtctt tcatttcatc gaagtgtttc tgtagtgcct gccaggcagc
    16981 ggtctgcgtt ggattgatgt ttttcattag caatactctt ctgattttga gaattgtgac
    17041 tttggaagat tgtagcgcca gtcacagaaa aatgtgatgg ttttagtgcc gttagcgtaa
    17101 tgttgagtgt aaacccttag cgcagtgaag catttattag ctgaactact gaccgccagg
    17161 agtggatgaa aaatccgcat gaccccgtcg ttgacaaccg ccccgctcac cctttattta
    17221 taaatgtact acctgcgcta gcgcaggcca gaagaggcgc gttgcccaag taacggtgtt
    17281 ggaggagcca gtcctgtgat aacacctgag ggggtgcatc gccgaggtga ttgaacggct
    17341 ggccacgttc atcatcggct acaggggctg aatcccctgg gttgtcacca gaagcgttcg
    17401 cagtcgggcg tttcgcaagt ggtggagcac ttctgggtga aaatagtagc gaagtatcgc
    17461 tctgcgccca cccgtcttcc gctcttccct tgtgccaagg ctgaaaatgg atcccctgac
    17521 acgaggtagt tatgtctgaa attgttgtct ccaaatttgg cggtaccagc gtagctgatt
    17581 ttgacgccat gaaccgcagc gctgatattg tgctttctga tgccaacgtg cgtttagtag
    17641 tcctctcggc ttctgctggt atcactaatc tgctggtcgc tttagcggaa gggctggaac
    17701 ctggcgagcg attcgaaaaa ctcgacgcta ttcgcaacat ccagtttgcc attctggaac
    17761 gtctgcgtta cccgaacgtt atccgtgaag agattgaacg tctgctggag aacattactg
    17821 ttctggcaga agcggcggcg ctggcaacgt ctccggcgct gacagatgag ctggtcagcc
    17881 acggcgagct gatgtcgacc ctgctgtttg tcgaaatcct gcgcgaacgc gatgttcagg
    17941 cacagtggtt tgatgtacgt aaagtgatgc gtaccaacga ccgatttggt cgtgcagagc
    18001 cagatgtagc cgcgctggcg gaactggccg cgctgcagct gctcccacgc ctcaatgaag
    18061 gcttagtgat cacccaggga tttatcggta gcgaaaataa aggtcgtaca acgacgcttg
    18121 gccgtggagg cagcgattat acggcagcct tgctggcgga ggctttacac gcatctcgtg
    18181 ttgatatctg gaccgacgtc ccgggcatct acaccaccga tccacgcgta gtttccgcag
    18241 caaaacgcat tgatgaaatc gcgtttgccg aagcggcaga gatggcaact tttggtgcaa
    18301 aagtactgca tccggcaacg ttgctacccg cagtacgcag cgatatcccg gtctttgtcg
    18361 gctccagcaa agacccacgc gcaggtggta cgctggtgtg caataaaact gaaaatccgc
    18421 cgctgttccg cgcgctggcg cttcgtcgca atcagactct gctcactttg cacagcctga
    18481 atatgctgca ttctcgcggt ttcctcgcgg aagttttcgg catcctcgcg cggcataata
    18541 tttcggtaga cttaatcacc acgtcagaag tgagcgtggc attaaccctt gataccaccg
    18601 gttcaacctc cactggcgat acgttgctga cgcaatctct gctgatggag ctttccgcac
    18661 tgtgccgggt ggaagtagaa gaaggtctgg cgctggtcgc gttgattggc aatgacctgt
    18721 caaaagcctg cggcgttggc aaagaggtat tcggcgtact ggaaccgttc aacattcgca
    18781 tgatttgtta cggcgcatcc agccataacc tgtgcttcct ggtgcccggc gaagatgccg
    18841 agcaggtggt gcaaaaactg catagtaatt tgtttgagta aatactgtat ggcctggaag
    18901 ctatatttcg ggccgtattg attttcttgt cactatgctt atcaataaac gagcctgtac
    18961 tctgttaacc aacgtcttta tcgagaataa ttgcctttaa tttttttatc tgcatctcta
    19021 attaattatc gaaagagata aatagttaag agaaggcaaa atgaatatta tcagttctgc
    19081 tcgcaaacga attcccgtac actgaactct ctggctcact gagggcacca tcatggcact
    19141 cccccgtatt acccaaaaag agatgactga acgcgagcag cgtgaactta aaacactgct
    19201 ggatcgggcg cggattgcgc atggtcgcgt actgaccaac tccgaaacca acagcattaa
    19261 gaaagagtac atcgataaat taatggtcga gcgtgaggct gaagcgaaaa aagcccgcca
    19321 attgaagaaa aagcaggctt ataaaccgga tcctgaagcg tcatttagct ggtcagcaaa
    19381 tacgtcgacg cgcggcaggc gctaatgaac aatcaacgcc tgagattttc actcgtcagt
    19441 gtgaacacaa ttttcccctc accctaaccc ttttcccgaa ggggcgaggg gactgtccga
    19501 gcacttttgg gccgttttca tcagtctgag gcgttaatca gcgccccttc tttttacgcc
    19561 ccggcgaggt aaaacgctta ccgttggacg ccgggcgacc gcctttttcc gccgtttttt
    19621 ccatcttaac gactggacgt ttgatgcccg ctgttttcgg tttggccttc gctttgggct
    19681 tcgcctcgga agaggaattt tcaatgagct taaagagatc gataagctcg tcgtcggtta
    19741 aatcgcgcca ttctcccagc ggaatgccgc ttaagctgac gttcataatg cgcgtacgtt
    19801 ccagcttttt cacttcatag ccgaaatgct cgcacatgcg ccggatctga cggttcaacc
    19861 cctgcaccag ggtaatgcgg aagacaaacg gtgcttcttt tttgactttg cactttttcg
    19921 tcaccgtccc gaggatcggc actcccgccc ccataccacg aataaattca tcggtaatcg
    19981 gtttatcgac cgtcaccaga tactctttct catgatcgtt acccgcacgg aggatcttat
    20041 tcaccagatc gccgtgattg gtgaggaaaa tcagcccctg ggagtcttta tccaggcggc
    20101 caatcgggaa cacgcgtttg ctgtggttaa cgaaatcgac aatgttatcg cgctcgccat
    20161 cttcggtggt gctgacaata ccgacgggct tgttcagggc gataagtacc aaatcttcgg
    20221 cttcccgagg ctcaatcaac tgaccgttta ctttcacaat gtcgccgggt ttcacctgat
    20281 caccaatggt ggctcgcttg ccattaagga acacattgcc ttgctcgata tagcgatccg
    20341 cttcgcggcg tgagcaaatt ccgctttcgc tgatgtattt atttaaacgg actgatgagt
    20401 cgggcagcat aaattctcct gtaaaacgcg gaatataccg tacctcttag cggggaaaaa
    20461 cagggttatg cggcaacgtc gaaattaaat gtgatctgcc acgcctttta tcacaaaagt
    20521 gtctccggaa gtacgatgca aatttgcctg gcaaaataag gataatcaac atattacgaa
    20581 tcgctgcaca cctgtgctga tgactactac gcagagaaac aaatgtgcaa gagaaagcgc
    20641 tatttctccc catgcaaaga gtgaaaaatc gctttgaaag gccctcataa tggggttata
    20701 tcagatcaag cggtgagaaa gcgtatttgc acaaatgtgc agaaccagga ggcggcaatg
    20761 gaaaacagtg acgatatccg tttgattgtg aagattgccc aactctatta cgaacaggat
    20821 atgacgcagg cgcaaatcgc gcgcgaactg ggtatttacc gcaccaccat cagccgcttg
    20881 cttaaacgag gccgcgatca gggaattgtc accatcgcca tcaactatga ctacaacgaa
    20941 aatctctggc tggagcagca actgaagcaa aagtttggct tgaaagacgt tgtggtggtg
    21001 tcgggaaatg atgaggatga agagactcaa ctggcgatga tggggttaca cggcgcgcaa
    21061 ctgctggatc gcttgctgga gcctggcgat attgtcggtt tttcctaggg tcgcgcggtg
    21121 agcgcactgg ttgaaaactt gccgcaagcg gggcaatcgc ggcagttaat ctgcgtgcca
    21181 attattggcg gaccgtccgg taaactcgaa agccgctatc acgtaaacac attaacctac
    21241 agcgtggcag cgaagctgaa aggggaatcg catctcgcgg attttccggc tctgctggat
    21301 aaccccttaa ttcgtaatgg gatcatgcag tctcagcact ttaaaaccat ctctgcctac
    21361 tgggataatc tggatgtcgc cctggtggga attggctcac cggccattcg cgacggcgct
    21421 aactggcatg cgttttatgg tggtgaagag agtgacgacc tgaaagcagg cgcggtattc
    21481 cattggcatt gccatgagtg aagaaaaata cagcggaatt gttggtgcac tgcgtggaaa
    21541 atatattaat tgtctggtaa cgaatagcag cacagctgaa ctattactga aataaaaatc
    21601 ttcccgatgt ttaccgtaat aaaaattacg gcgggtccct attatctaaa atcaggagtc
    21661 tgttatgcaa acgtggttaa atttgcagga taaaatcatt atcgtcaccg gcggcgcatc
    21721 cggtattggt ctggcgattg tagaggaatt attagcacaa ggcgcaaacg tacagatggt
    21781 agatattcac ggtggcgatg gtcaatatga agttcataaa ggctatcagt tttggccgac
    21841 cgatatttcc agcgccaaag aggtaaatca tacggtagcg gaaattatcc agcgttttgg
    21901 tcgcatcgac ggtctggtca ataacgccgg ggtcaatttc ccgcgtctgc tggtcgatga
    21961 gaaagcgcct gccgggcagt atgaactcaa cgaagctgca ttcgaaaaaa tggtcaatat
    22021 caaccaaaaa ggcgtttttc tgatgtcgca ggcggtggcg cgccagatgg tcaaacaaca
    22081 tgatggcgtg attgtgaatg tttcctcaga aagtggactg gaaggctcag aaggccaaag
    22141 ctgttacgcc gctaccaaag ccgcgctcaa tagcttcacg cgctcctggt cgaaagagct
    22201 gggtaaacac ggtatccgtg tggtcggtat cgcgccgggg attctggaaa aaaccgggct
    22261 acgcacgccg gaatatgaag aggcgctggc gtggacgcgc aatatcaccg tcgagcagct
    22321 gcgtgaaggc tataccaaaa acgccattcc tattgggcgc gccggaagat tagcagaagt
    22381 cgctgatttt gtttgttatc tgctgtctga acgcgccagc tacatcaccg gagtaaccac
    22441 taacattgcg ggcggcaaaa cgcgcggata aggaggcttt atggtcaacg ccattttttg
    22501 tgcccacggc aaattagctt gcgccatgct ggaatcggtt cagatggtgt acggcgacgc
    22561 caatgtcgag gcggttgaat ttgtaccagg cgagaacgcc ggagacatcg tagcaaagct
    22621 ggaaaagtta gtaagcattc acaatcaaga tgagtggctg attgccgtgg atttacagtg
    22681 cggcagcccg tggaatgcgg cagcgatgct tgcgatgcgc aatcctcgtc tgcgggtaat
    22741 cagcggcctt tccctgccgc tggcgctcga actggttgat aaccaggaca gcatgaatgt
    22801 tgatgaactg tgtgaacacc tgacgcaaat cgcaaagcaa acctgcgttg tctggaaaca
    22861 actggcaacg gcagaggaag atttctgatg aacattacgc tcgcccgcat tgatgaccgt
    22921 ctgatccacg gccaggtcac taccgtctgg tcgaaagtgg ctaacgccca gcgcattatt
    22981 atctgcaatg acgaagttta taacgatgaa gtgcgccgga cattattgcg tcaggctgcg
    23041 ccaccgggaa tgaaggttaa cgtggtcaat attgaaaaag ccgtcgcggt ttatcacaac
    23101 ccgcaatatt aggacgaaac cgttttttat cttttcactc gcccacagga tgccttagcg
    23161 atggttcgcc agggggtaaa aataggcacc ctgaatattg gcggcatggc ctggcggccc
    23221 ggtaaaaaac agttaaccaa agcggtttca ttagatgatg atgatattaa cgcatttcat
    23281 gagttaaata atcttggcgt cataatggat ttacgcgtcg tagcgtcaga tccctcaatc
    23341 aatattatcg acaaaataaa cgaacagtta atcgcaaatt aaataatagc gcctgtgtac
    23401 atatgcttta acaggcaggg atgacgacac ttaaaaggtg acaaatcatg gaaataagta
    23461 ccctacaaat tattgctata tttctttttt cctgtattgc cggaatgggc agcgtgctgg
    23521 atgaatttca gactcatcgc ccgttaattg cctgtacggt gattggttta attctcggtg
    23581 atttaaaaac cggaataata ctcggtggta cgctggaatt gatagctctc ggctggatga
    23641 acgtcggcgc ggcgcaatct ccggattctg cactcgccag cataatctcc gccattctgg
    23701 ttatcgttgg tcagcaaagc atcgccaccg gaatcgccat cgcgttgcct gtggctgcgg
    23761 caggccaagt gctgacggtg tttgcccgta ccatcaccgt agtgttccag cacgcggcgg
    23821 ataaagcagc ggaagaggcg cggtttcgca ctctcgatat tctgcatgtc tccgcgcttg
    23881 gcgtgcaggc gctgcgcgtt gctattccgg cactgattgt ctcactgttc gtcagcgccg
    23941 atatggtcag caatatgtta aacgccattc ccgaatttgt gacccgtgga ctgcaaattg
    24001 ctggcggttt tatcgtggtg gtcggttacg ccatggtgct acgtatgatg ggcgtgaaat
    24061 atttgatgcc tttctttttc ctcggtttcc tcgcaggtgg ctacctcgat ctcagcctgc
    24121 tggcgttcgg tggcgtcggc gtgatcatgg ccctggtcta catccagcta aatccacagt
    24181 ggcgtaaagc tgaacctcaa ctccaggcca ccgcctccac cgcccttgac caacttgacg
    24241 attaacggag ccatcatgga acagagaaaa attacacgca gcgatctggt gagcatgttt
    24301 ctgcgctcca acctgcaaca ggcgtccttt aactttgaac gtattcacgg actgggcttt
    24361 tgctacgaca tgatccccgc catcaagcga ctttacccat taaaagagga tcaggttgcg
    24421 gcgctcaggc gacacctggt gttcttcaat accacgccag ccgtatgtgg cccggtcatc
    24481 ggcgtcactg ccgccatgga agaggcgcgg gccaacggcg cggaaattga tgacggtacc
    24541 attaacggca tcaaagtcgg tctgatggga ccattggcag gagttggcga tccactggtc
    24601 tggggaacgc tgcgcccgat taccgctgcg ctcggcgcat ctctggcact ttctggcaac
    24661 atcctcggcc cgctgctgtt tttctttatt ttcaatgcgg tgcgtctggc gatgaagtgg
    24721 tatggcctac agctcggctt tcgcaaaggg gtgaatatcg tcagcgatat gggcgggaat
    24781 gtgctgcaaa aactcaccga aggcgcgtcg attctcggac tgtttgtgat gggcgtgctg
    24841 gtcaccaaat ggacgtcaat caacgtaccg ttggtggttt cacaaacgca tgccgccgat
    24901 ggctccaccg tcatcatgac cgtgcagaac attctcgacc aactttgccc tggtttgctg
    24961 gcgctcggtc tgacgctact gatggttcgt ctgctcaaca aaaaaattaa cccggtatgg
    25021 ctgattttcg ccctgtttgg cttagggatt atcggcaatg cgctgggctt cctgtcctga
    25081 ttcttcgccc cggcacgact gccggggcca tcgctcaaca tgaggtggtt tatgaaaaca
    25141 acagctctgc gtctttatgg taaacgtgat ttacgcctgg aaacctttga ccttcctgaa
    25201 atgcaggagg atgaaatcct cgcgacggtg gtcactgaca gcctgtgcct ctcttcctgg
    25261 aaagaggcaa cctgggtgaa aaccataaaa aagtacccga cgatgtggcg accaacccaa
    25321 tcatcatcgg ccacgagttt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca
    25381 aattccagcc gggtcagcgt tatgtgattc aggccaacct gaaactccct gaccgcccgg
    25441 actgccccgg ctactccttc ccgtgggtgg gcggcgaagc cacgcatgtg gttattccca
    25501 acgaggtcat ggaacaagat tgcctgctgg catacgacgg cgaaacctat tttgaaggct
    25561 cgctggttga accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg
    25621 aaggtagtta taaccacacg atggggattc gcccgcaagg gcgcacgctg atcctcggcg
    25681 gcaccggacc aatgggattg ttggcgattg attatgcgct gcatggacca gttaacccgt
    25741 cgctgctcgt cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc
    25801 catcagaacc gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt
    25861 taatggcgct gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag
    25921 gactggtgac tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg
    25981 gaccgcagga taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta
    26041 cccactacgt gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga
    26101 ttgaagagaa aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg
    26161 cgggcgaaac cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg
    26221 ggaaatacct gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc
    26281 tggcgcgtca tcaggggatc tggtccggtg aggcggagca atatctgctc actcatgcag
    26341 aggcaatttc ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgccgc
    26401 ggcaacttcg cgacctgatg tgcggctgat tgctgaagcg gcccaatgtg cacgagaagg
    26461 ataaaaaaac atcagcgcct taaggggcgc tcaaattggt ggcaaagtac acattgttta
    26521 tgccggatgc ggaataaagc cttattcggc ctacataatc tcgaaaattc aatatattgc
    26581 agttatctgg taggcctggt aagcgtagcg catcaggcaa tgctgtgttt gtcatcagtc
    26641 tcagcgcccg gtgcaatacg tgacatcgtg agccatgtca gtaagtggtt aaattcgcag
    26701 tggtagcatg ccttccgtga tttcttacta accggagaag taatggaact gcttttattg
    26761 agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct aattgctgaa
    26821 cagttgcagg gtcgccgctc agcggtgttt atccctttcg ctggcgtaac gcagacctgg
    26881 gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc tgtcaccggt
    26941 attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg ctgagatcgt gattgtcggg
    27001 ggcgggaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct ggcaccaatt
    27061 actgacgtgg ttaaacgtgg tgcgctgtat attggctgga gcgcaggcgc taaccttgct
    27121 tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg tttcgatgcg
    27181 ctaaatctgt tcccgctgca aatcaacccg cacttcacca acgcgctgcc ggaaggccat
    27241 aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc agaactgacg
    27301 attattggtc taccggaagg taactggatc acggtgagta aaggtcacgc tacgctgggt
    27361 ggcccgaaca ccacttatgt gtttaaggcc ggtgaagaag cggtgccgct ggaagcgggt
    27421 caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata gtgcctgatg
    27481 cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact cgtcccgtcc
    27541 ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca gcgagttcag
    27601 acgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc tctgcacgtt
    27661 ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaagcg
    27721 atgcttgctg cggcgcaaac gacgagcgct ggtgacatcg ccagagaaga acaccgacat
    27781 tgccagcttt aaattactga gcaattgctc atagagcgca tccagttcct tcaacccatc
    27841 aagcgaaaat gcccgccgtg ctgccagcga tttatcagca atttcgctgc ccatgcgctc
    27901 gacgatatcg gaagcctgtt caaggttgag cgacatttca ataatctccg cccagcgacg
    27961 cgactcttct tctgccagtt cctcttttgg cattcgcgcc agatacagct taatggcggt
    28021 gtagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc gtggctcgcc
    28081 gtgcatcact ttattcagcc cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt
    28141 ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga catccagatg
    28201 tttaggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg
    28261 tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt agaagacgtg
    28321 gaaatagatc accagttccg cttttggcaa tgacaacttc cccattgtct ctgccagcaa
    28381 atggacaaac ggcaggataa tcaggctacc caccagctta aacagcaaac tacccagcgc
    28441 gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca ggccggaacc
    28501 gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc ctgcggcggt
    28561 cagagtcgcg gtcagcagta cagcagcaag gctggagtag ctgataatgg cgaacatcgc
    28621 gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca cctgaacgcc
    28681 gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca gcaaaatcag
    28741 cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa
    28801 aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga aggtgagaat
    28861 acgcgccatt agcgccgtcc cgacatctgc acccagcaca atcaccagag ccggtgcgag
    28921 ggctaccaga tcctgagcga cgaacgaggt caccagcatg gtggtggcat tactgctctg
    28981 taccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt cgacgctccg
    29041 gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg
    29101 agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta acacgagttc
    29161 aggttctcct tatcgttata ttttcctgaa gcaattgaat gaccaaagga ccggaaacac
    29221 gctgactggc atctttactg tgccttttga cattcgtttt tccggctaag attattccag
    29281 cctgtttcca gtataagtgt ttcatattaa taaagagaca gggcactcat tgagcgccct
    29341 gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc ccagattcgg
    29401 tgccagccag cgctcaacat cggagacgct cataccttta cggcgggcat agtcttcaac
    29461 ctgatcgcgc tgaatttgcg ccaccgcgta gtacttgctg tccgggtggc tgaaatacca
    29521 acccgaaacc gatgcaccag gccacatggc gaaagattct gtgagtttca tgccagtatg
    29581 tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg ggcaggccgg
    29641 gtagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt cgttgctgag
    29701 gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga gatactccgc
    29761 aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt aatcatcgtg
    29821 ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc
    29881 gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga gacagtagtt
    29941 agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga tcacatgggt
    30001 gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg ggaacaagcc
    30061 caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca tgtcgttggc
    30121 gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa tgcgcggata
    30181 cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat tacgcagagt
    30241 ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc
    30301 ctgccagtca aaagcgaagt cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg
    30361 cggtttcttg cgcccgtgct gaatacgtac ggtttcatac tccttgcggg tacgagcgac
    30421 aaaatcatcg cgctgagtat cggaaagcag cgccgccacc acaccaacgg tgcgcgaggc
    30481 attctgcaca tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc
    30541 ttttgaggtc gtcgcgccac cgatcagtaa cggaatggtg aagccctgac gctccatctc
    30601 tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa gtccaatcag
    30661 atcagcattc acttctttag cggtacggag aattttttcc gcaggcacca taacgccgag
    30721 atcgacaatt tcgtagttgt tacattgcag caccacacca acgatatttt taccgatgtc
    30781 gtggacgtcg cccttcacgg tggcgatcac catcttgccg ttggttttgc cctgctcttt
    30841 gctggcttca ataaacggtt cgaggtaggc cactgcctgt ttcatgacgc gcgccgattt
    30901 gaccacctgt ggcaggaaca ttttcccctc gccaaacaga tcgccgacca cgttcatgcc
    30961 gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc
    31021 ggtatcctgc tcgataaact cagtaatgcc tttgaccagc gagtattcca gacgtttatt
    31081 cacttcccac gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg ttttgctgcc
    31141 gcgatatttc tcggcaagct ccagtaaacg ttcagtgcca tcatcgcgac gattaagaat
    31201 cacatcttcc accgcgtcgc gcagttcagc gggcaggtcg tcgtaaatcg ccagttgccc
    31261 ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga acaccgcgtg
    31321 aatggcttcg cgcaccggtt cgttgccacg gaatgagaaa gaaacgttag atacgccgcc
    31381 ggaaatcagc gcgtgcggca gttcgcgttt gatgtcttcg caggcgccga taaagtcctg
    31441 cgcatagttg ttgtgctctt caatgccggt ggcgaccgcg aagatgttcg ggtcgaagat
    31501 gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat
    31561 ctcgattttc cgtgcgcggg tatcggcctg tccctgttcg tcaaaggcca ttaccaccac
    31621 cgctgcaccg taacgacgca acagtttcgc atgatggata aaggcgtcga cgccctcttt
    31681 cattgagata gagttaacaa tgcctttgcc ctgaatacac ttcagcccct tttcgatgac
    31741 ctcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat
    31801 cagattgaga aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat ccatattgat
    31861 atcgataatc tgcgcgccgt tttccacctg ctggcgggcc acatccagtg cctcgctgta
    31921 tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg tgcgttcacc
    31981 cacgttcaca aacaggctat cttcgccaat gttcagcggc tccaggccgg acaaacggca
    32041 ggctacgggg atttccggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc
    32101 tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa agcccgcttc
    32161 cgcccattca cgtatttgtt ttgccatcgt gtcggcgtcg agatcgtact caccaaaggc
    32221 gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc gtgacagctc
    32281 ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggtcagcgc
    32341 ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc
    32401 ggaggcgtcg gtgatggtgc cagagatcat aatcggcagc tcaacgccca gcgtttcaaa
    32461 ctccgttttt accgcaaata ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat
    32521 caggatcaga tccgcaccac cttccaccag cgctttggtg gactctcgat aagccgccac
    32581 cagcccgtca aaagtgatat tacgaaatgc cggatcgttg acgtccggag aaatagacgc
    32641 cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc
    32701 ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga
    32761 cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc
    32821 gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt
    32881 actgagtacc agcaggtcgt tgttgccttt gaggtcgcat ggccagtcgg caaagcgttc
    32941 accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca taccgccgtc
    33001 cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact
    33061 tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac
    33121 gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg
    33181 atcagttcag caactattgc attaactaac aataaaaatg aaaatgattt ccacgataca
    33241 gaaaaaggag actgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt
    33301 tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga
    33361 gtggattgcc gaatccaatg gcagtgtggc actcacggaa ctggcgcaac aagccgggtt
    33421 acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt tcgtgcgtca
    33481 ggttggcgaa ctgggacact gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt
    33541 tctccagagc cgtaatttgt tagcgattgt tcaccctatc ctgcgcaatc taatggaaga
    33601 gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat
    33661 cgatcaggta cagtgtactc acctgatgcg tatgtccgca cctatcggcg gtaaattgcc
    33721 gatgcacgct tccggtgcag gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac
    33781 gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt
    33841 gcatttaaaa gaagatctcg cccaaacgcg caaacggggc tattcatttg acgatgagga
    33901 acatgcactg gggctacgtt gccttgcagc gtgtattttc gatgagcacc gtgaaccgtt
    33961 tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt
    34021 tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg
    34081 actttttctg gcgggcagag gcaatattct gcccatcata cctgagtggc aatagaataa
    34141 gggtgtctgt taatcgcatt gacgccaaaa taacttaatg tcatacactt cacacacttt
    34201 tattaactca gcattatttt taaacatcaa accacttaat tattaacaaa atcgcaagat
    34261 ctcattccac cgaaact
//
LOCUS       DAZPEP010000028        34135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZPEP010000028 DAZPEP010000000
VERSION     DAZPEP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.028"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            38..580
                     /locus_tag="ACHUT9_001339"
     CDS             38..580
                     /locus_tag="ACHUT9_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1347856.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="ACHUT9_001340"
     CDS             663..1364
                     /locus_tag="ACHUT9_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1347857.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="ACHUT9_001341"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="ACHUT9_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="ACHUT9_001342"
     CDS             3979..5049
                     /locus_tag="ACHUT9_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1347858.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="ACHUT9_001343"
     CDS             5061..5603
                     /locus_tag="ACHUT9_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1347859.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="ACHUT9_001344"
     CDS             5611..6126
                     /locus_tag="ACHUT9_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1347860.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="ACHUT9_001345"
     CDS             6092..6829
                     /locus_tag="ACHUT9_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1347861.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUT9_001346"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="ACHUT9_001346"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFR1347862.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUT9_001347"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="ACHUT9_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFR1347863.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUT9_001348"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="ACHUT9_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFR1347864.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUT9_001349"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="ACHUT9_001349"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFR1347865.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNLGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="ACHUT9_001350"
     CDS             12136..14043
                     /locus_tag="ACHUT9_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1347866.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUT9_001351"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="ACHUT9_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFR1347867.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUT9_001352"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="ACHUT9_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFR1347868.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUT9_001353"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="ACHUT9_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFR1347869.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUT9_001354"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="ACHUT9_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFR1347870.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUT9_001355"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="ACHUT9_001355"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFR1347871.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="ACHUT9_001356"
     CDS             complement(18711..20471)
                     /locus_tag="ACHUT9_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1347872.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="ACHUT9_001357"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="ACHUT9_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFR1347873.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUT9_001358"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="ACHUT9_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFR1347874.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHUT9_001359"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="ACHUT9_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFR1347875.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="ACHUT9_001360"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="ACHUT9_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFR1347876.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHUT9_001361"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="ACHUT9_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFR1347877.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="ACHUT9_001362"
     CDS             complement(26072..26518)
                     /locus_tag="ACHUT9_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFR1347878.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="ACHUT9_001363"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="ACHUT9_001363"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFR1347879.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHUT9_001364"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="ACHUT9_001364"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFR1347880.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="ACHUT9_001365"
     CDS             complement(29281..29943)
                     /locus_tag="ACHUT9_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFR1347881.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30116..30529
                     /locus_tag="ACHUT9_001366"
     CDS             30116..30529
                     /locus_tag="ACHUT9_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFR1347882.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHUT9_001367"
     CDS             complement(30574..30891)
                     /gene="hspQ"
                     /locus_tag="ACHUT9_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFR1347883.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHUT9_001368"
                     /pseudo
     CDS             complement(30949..32139)
                     /gene="rlmI"
                     /locus_tag="ACHUT9_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            32234..32512
                     /gene="yccX"
                     /locus_tag="ACHUT9_001369"
     CDS             32234..32512
                     /gene="yccX"
                     /locus_tag="ACHUT9_001369"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFR1347884.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHUT9_001370"
     CDS             complement(32509..32838)
                     /gene="tusE"
                     /locus_tag="ACHUT9_001370"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFR1347885.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHUT9_001371"
     CDS             complement(32929..33588)
                     /gene="yccA"
                     /locus_tag="ACHUT9_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFR1347886.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33795..33882)
                     /locus_tag="ACHUT9_001372"
     tRNA            complement(33795..33882)
                     /locus_tag="ACHUT9_001372"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33846..33848),aa:Ser,seq:tga)
BASE COUNT         8830 a         8131 c         8899 g         8275 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggcca aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaaa aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc gtgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcttggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggatccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctgtgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 catacttact aacatataga catatttccc gcttttgctc tcattcattc gtattagctg
    30061 catggttggc atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa
    30121 agaaaccgat attgcaggca ttttaacgtc cactcacacc attgcgctgg tgggggcaag
    30181 cgacaaaccc gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca
    30241 cgtcattccg gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg
    30301 cacgctggca gacgttccgg aaaaggtcga tatggtcgat gttttccgta attcagaagc
    30361 ggcgtggggc gtggcacagg aagctattgc cattggcgcg aaaaccctct ggatgcaact
    30421 gggggtgatt aatgagcaag cggcagtact ggcacgggat gccgggttga atgtggtgat
    30481 ggatcgctgc ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg
    30541 aaggcaaaaa ccttccgggg attcgttcag ggatcagtta cgcagacgcg gggcctggag
    30601 ttgtttgcgg atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg
    30661 caattcgcta cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc
    30721 ctccatcact acgtgatacc acggagcggc gcgaagctcg tcatttaccg ccagctcatc
    30781 aggcgacggt tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc
    30841 taacagggaa tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc
    30901 ccgggaaatc tttacctttg atataagggt aaaattccac atttcaagtt acatgacgcg
    30961 acaggcaaac cctttcagat atagcccttc cggataggta gcgatcaccg gatgatcggc
    31021 tgcctgacgg aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc
    31081 gatgattttc tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat
    31141 accgccttca ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc
    31201 gcccatcaac tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa
    31261 tttttcaccg cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc
    31321 agccttgctc agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc
    31381 ctaggaggta tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa
    31441 accaccggta taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc
    31501 caggcggctg tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac
    31561 cagcagcttc atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac
    31621 gaggccctgg gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta
    31681 aatcgcacat tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatattc
    31741 tgccccagca ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc
    31801 cggcaggcca tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc
    31861 cagtcagtca cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat
    31921 agactcagac gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc
    31981 gccgcgtgct aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc
    32041 tttaccttcc atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga
    32101 tttttcgcgc cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc
    32161 aaagaaatgg gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat
    32221 aaggagagca gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg
    32281 cgtaggattt cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc
    32341 caaaaatctt gacgatggca gcgtggaagt ggttgcctgc ggtgaagaag ggcaggtgga
    32401 aaaattaatg cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct
    32461 cagcgagccg catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt
    32521 taccggtttt ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag
    32581 tcggtataaa tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac
    32641 cagcatacga atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac
    32701 cacttcccag tgttccggtg acagcgaaat cccttcgttc tctgcaatca ccactgccag
    32761 cggctcactc cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt
    32821 accttcgaag atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa
    32881 aacaaagccc cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct
    32941 ggcgaagccc agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct
    33001 aaccgtggca cgaatatagt tcgtttcacc gccatgaacg atgttgctgg tttcaaacaa
    33061 aatagcgcca gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag
    33121 ctgcaggaag atattagcaa ccataccaat cagcaccacc acaatacccg ccatcagcat
    33181 accgccgagg aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa
    33241 cactaacgcc gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata
    33301 ggtgttcaga ataggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat
    33361 aatcccggtc ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac
    33421 cagcgtcaga atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat
    33481 cgccgaaaag gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt
    33541 gctaagcagt gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc
    33601 tatgacagat gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt
    33661 tacccatctt tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag
    33721 caaatataac gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa
    33781 ccccttatct tatttggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc
    33841 ggttttcaag accggtgcct tcaaccgctc ggccacactt ccggaatgac gcgcactata
    33901 aacatcccga tgcggcgtgt aaacccctaa tttgtttgtt tgcctgaaaa acagccaaaa
    33961 gtgcattgat agcgtgaaat aacagcagat tgatcatttc atcaccatga attccttctc
    34021 ttttactcgt ttagcaaccg gctaaacatc cccaccgccc ggcaaaaaga aaaataggtc
    34081 catttttatc gctaaaagat aaatccacac agtttgtatt gttttgtgca aaagt
//
LOCUS       DAZPEP010000029        33562 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZPEP010000029 DAZPEP010000000
VERSION     DAZPEP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33562
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.029"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            376..825
                     /gene="alaE"
                     /locus_tag="ACHUT9_001373"
     CDS             376..825
                     /gene="alaE"
                     /locus_tag="ACHUT9_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFR1347887.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
     gene            complement(862..1206)
                     /locus_tag="ACHUT9_001374"
     CDS             complement(862..1206)
                     /locus_tag="ACHUT9_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFR1347888.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1358..1687
                     /locus_tag="ACHUT9_001375"
     CDS             1358..1687
                     /locus_tag="ACHUT9_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFR1347889.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1936..2181
                     /gene="nrdH"
                     /locus_tag="ACHUT9_001376"
     CDS             1936..2181
                     /gene="nrdH"
                     /locus_tag="ACHUT9_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFR1347890.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2178..2588
                     /gene="nrdI"
                     /locus_tag="ACHUT9_001377"
                     /pseudo
     CDS             2178..2588
                     /gene="nrdI"
                     /locus_tag="ACHUT9_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            2561..4705
                     /gene="nrdE"
                     /locus_tag="ACHUT9_001378"
     CDS             2561..4705
                     /gene="nrdE"
                     /locus_tag="ACHUT9_001378"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1347891.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVQSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4715..5674
                     /gene="nrdF"
                     /locus_tag="ACHUT9_001379"
     CDS             4715..5674
                     /gene="nrdF"
                     /locus_tag="ACHUT9_001379"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1347892.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6030..7232
                     /gene="proV"
                     /locus_tag="ACHUT9_001380"
     CDS             6030..7232
                     /gene="proV"
                     /locus_tag="ACHUT9_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFR1347893.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7225..8289
                     /gene="proW"
                     /locus_tag="ACHUT9_001381"
     CDS             7225..8289
                     /gene="proW"
                     /locus_tag="ACHUT9_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFR1347894.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8347..9339
                     /gene="proX"
                     /locus_tag="ACHUT9_001382"
     CDS             8347..9339
                     /gene="proX"
                     /locus_tag="ACHUT9_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFR1347895.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9630..10814
                     /locus_tag="ACHUT9_001383"
     CDS             9630..10814
                     /locus_tag="ACHUT9_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1347896.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10938..11675
                     /gene="ygaZ"
                     /locus_tag="ACHUT9_001384"
     CDS             10938..11675
                     /gene="ygaZ"
                     /locus_tag="ACHUT9_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFR1347897.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11665..12000
                     /gene="ygaH"
                     /locus_tag="ACHUT9_001385"
     CDS             11665..12000
                     /gene="ygaH"
                     /locus_tag="ACHUT9_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFR1347898.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12091..12621
                     /gene="emrR"
                     /locus_tag="ACHUT9_001386"
     CDS             12091..12621
                     /gene="emrR"
                     /locus_tag="ACHUT9_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFR1347899.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12748..13920
                     /gene="emrA"
                     /locus_tag="ACHUT9_001387"
     CDS             12748..13920
                     /gene="emrA"
                     /locus_tag="ACHUT9_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFR1347900.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYCFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13937..15475
                     /gene="emrB"
                     /locus_tag="ACHUT9_001388"
     CDS             13937..15475
                     /gene="emrB"
                     /locus_tag="ACHUT9_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFR1347901.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15539..16054)
                     /gene="luxS"
                     /locus_tag="ACHUT9_001389"
     CDS             complement(15539..16054)
                     /gene="luxS"
                     /locus_tag="ACHUT9_001389"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFR1347902.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16204..17760)
                     /gene="gshA"
                     /locus_tag="ACHUT9_001390"
     CDS             complement(16204..17760)
                     /gene="gshA"
                     /locus_tag="ACHUT9_001390"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFR1347903.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            complement(17833..18261)
                     /locus_tag="ACHUT9_001391"
     CDS             complement(17833..18261)
                     /locus_tag="ACHUT9_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFR1347904.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18258..18824)
                     /gene="yqaB"
                     /locus_tag="ACHUT9_001392"
     CDS             complement(18258..18824)
                     /gene="yqaB"
                     /locus_tag="ACHUT9_001392"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFR1347905.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19105..19181)
                     /locus_tag="ACHUT9_001393"
     tRNA            complement(19105..19181)
                     /locus_tag="ACHUT9_001393"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19145..19147),aa:Arg,seq:acg)
     gene            complement(19380..19456)
                     /locus_tag="ACHUT9_001394"
     tRNA            complement(19380..19456)
                     /locus_tag="ACHUT9_001394"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19420..19422),aa:Arg,seq:acg)
     gene            complement(19520..19596)
                     /locus_tag="ACHUT9_001395"
     tRNA            complement(19520..19596)
                     /locus_tag="ACHUT9_001395"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19560..19562),aa:Arg,seq:acg)
     gene            complement(19600..19692)
                     /locus_tag="ACHUT9_001396"
     tRNA            complement(19600..19692)
                     /locus_tag="ACHUT9_001396"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19656..19658),aa:Ser,seq:gct)
     gene            complement(20008..20193)
                     /gene="csrA"
                     /locus_tag="ACHUT9_001397"
     CDS             complement(20008..20193)
                     /gene="csrA"
                     /locus_tag="ACHUT9_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFR1347906.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20428..23058)
                     /gene="alaS"
                     /locus_tag="ACHUT9_001398"
     CDS             complement(20428..23058)
                     /gene="alaS"
                     /locus_tag="ACHUT9_001398"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFR1347907.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23186..23686)
                     /gene="recX"
                     /locus_tag="ACHUT9_001399"
     CDS             complement(23186..23686)
                     /gene="recX"
                     /locus_tag="ACHUT9_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFR1347908.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(23914..24975)
                     /gene="recA"
                     /locus_tag="ACHUT9_001400"
     CDS             complement(23914..24975)
                     /gene="recA"
                     /locus_tag="ACHUT9_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFR1347909.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25055..25552)
                     /gene="pncC"
                     /locus_tag="ACHUT9_001401"
     CDS             complement(25055..25552)
                     /gene="pncC"
                     /locus_tag="ACHUT9_001401"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFR1347910.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(25697..26782)
                     /gene="mltB"
                     /locus_tag="ACHUT9_001402"
     CDS             complement(25697..26782)
                     /gene="mltB"
                     /locus_tag="ACHUT9_001402"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFR1347911.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            27038..27601
                     /gene="srlA"
                     /locus_tag="ACHUT9_001403"
                     /gene_synonym="gutA"
     CDS             27038..27601
                     /gene="srlA"
                     /locus_tag="ACHUT9_001403"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFR1347912.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            27598..28557
                     /gene="srlE"
                     /locus_tag="ACHUT9_001404"
                     /gene_synonym="gutE"
     CDS             27598..28557
                     /gene="srlE"
                     /locus_tag="ACHUT9_001404"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFR1347913.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            28568..28939
                     /gene="srlB"
                     /locus_tag="ACHUT9_001405"
                     /gene_synonym="gutB"
     CDS             28568..28939
                     /gene="srlB"
                     /locus_tag="ACHUT9_001405"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFR1347914.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            28943..29711
                     /gene="srlD"
                     /locus_tag="ACHUT9_001406"
                     /pseudo
     CDS             28943..29711
                     /gene="srlD"
                     /locus_tag="ACHUT9_001406"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            29817..30176
                     /gene="gutM"
                     /locus_tag="ACHUT9_001407"
     CDS             29817..30176
                     /gene="gutM"
                     /locus_tag="ACHUT9_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFR1347915.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30243..31016
                     /gene="srlR"
                     /locus_tag="ACHUT9_001408"
                     /gene_synonym="gutR"
     CDS             30243..31016
                     /gene="srlR"
                     /locus_tag="ACHUT9_001408"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFR1347916.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            31009..31974
                     /gene="gutQ"
                     /locus_tag="ACHUT9_001409"
     CDS             31009..31974
                     /gene="gutQ"
                     /locus_tag="ACHUT9_001409"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFR1347917.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31971..33485)
                     /gene="norR"
                     /locus_tag="ACHUT9_001410"
     CDS             complement(31971..33485)
                     /gene="norR"
                     /locus_tag="ACHUT9_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFR1347918.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
BASE COUNT         7925 a         8934 c         8430 g         8273 t
ORIGIN      
        1 actggtgctt tctgctgtta atgtacattt tctggcatca aaaatgctgt aaatgacacg
       61 gtcacgacat acaccgaatt ctctacgctt atagcgttac ctcaccccca aactttcatt
      121 agtgaaatta aaggcaaggt catatctaat tgatttaatt gaaaataaat catatcggca
      181 tgtaaaaatc cacaatgtac aaaaaacgag ccgttacgga atattttatc tacaaaaact
      241 gactaaataa aaatttttca ctaattgaat agtcatagcc agcgatatac gctatgcgaa
      301 aatgcagatg gcaatgagat ccactgcttt catctccatt aacatcccat tacgctttta
      361 ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc gtcatgcagt tgcagatacg
      421 ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta ttgaagtttt cctctccgga
      481 atgagcttcg aacagtcttt ttattccaga ttggtagcga ttccggtgaa catcttaatt
      541 gcatggccat acggtatgta ccgcgatcta tttatgcgcg cggcacgcaa agttagcccg
      601 tcgggctgga taaaaaatct ggctgatatc ctggcttatg tgacgttcca gtcaccggtg
      661 tatgtggcga tcttgttagt ggtgggcgca gactggcatc agattatggc ggcggtcagt
      721 tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg gctacttcct cgattattgc
      781 cgccgactgt ttaaagtcag ccattaccag caggtaaaag cctgactctt cttttcgcga
      841 ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat aaaccattca aataacgttc
      901 aagcgcaaca cgcgaactga agccatgcgg gataccgtaa tgctcgtgtc gctcgcctgc
      961 taaatagagc ggaaattgtt gataatgatc gcaagtttta atatcggaag cgggttcagc
     1021 cagtgtcgaa ctacgttctt ttaatgttgg atgaattacc agtgcggtac gtcccattcg
     1081 cgcttcgcga ttaacataga cataattttc cccacggcgg taaccataag ctttctgggt
     1141 taccacatcg acagtaaaac cgactttttc aagtacgcgc gccacctcgt ctggtcgtaa
     1201 atacatattt gatcctcgtt attatcaacc gcgggcctac cttacctgat tgcgcattca
     1261 caacgctttc agaaaagtcc ataaagccgc gatgaacctc agttaagaaa attatggtct
     1321 acactgaaaa ttacatcgaa ttctaatggg ggatcatatg tttaaccgac cgaaccgcaa
     1381 cgacgttgat gatggcgtgc aggatatcca gaatgatgtc aatcaattag ctgacagcct
     1441 cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg gaagctgaag ccgcacgcag
     1501 taaagctcag gcattgctga aagaaaccag ggcgcgaatg catggtcgta ctcgcgtcca
     1561 gcaagccgca cgcgatgccg ttggctgcgc ggattctttt gttcgtgaaa gaccctggtg
     1621 tagcgtgggt acagcagctg cggtaggtat ttttatcggc gcactgttaa gcatgcgcaa
     1681 atcgtagtgc aaaaatgata ataaatacgc gtctttaacc ccgaagcctg tcttcggggt
     1741 ttctttttgc ctggtgaatc acaaaaatcc ccctcccccg tcacgctcat atctagggta
     1801 atttcgatca ctatttgcta tatattgtgt ggttgaatct ttttttcaac tacatctagt
     1861 atctctgtat caacagagag acaacccgac gcgtatcatc gcgccgtatc ttcattttaa
     1921 acggaaatac gaatcatgcg cataactatt tacactcgta acgattgcgt tcagtgccac
     1981 gccaccaaac gggcgatgga aaaccggggc tttgattttg aaatgattaa tgtcgatcgc
     2041 gttcctgaag cagcggaagc gttgcgtgct cagggctttc gtcagttgcc ggtagtgatt
     2101 gctggcgatc ttagctggtc tggtttccgt ccggacatga ttaaccgtct gcatccagcg
     2161 ccacacgcgg ccagtgcatg agccagctcg tctacttctc cagcagctcc gaaaacacgc
     2221 agcgttttat cgaacgttta ggtctgcccg cggtgcgcat cccgctcaat gagcgggaac
     2281 ggattcaggt agacgagcct tacatcctga tcgtgccctc ttacggcggc ggcggtacgg
     2341 ctggcgcggt gccacgatag gtaattcgct ttttaaacga cgagcacaac cgggcgttgc
     2401 ttcgcggcgt tattgcttct ggtaatcgca actttggtga ggcgtatggc cgcgccggag
     2461 atgtgattgc ccggaaatgc ggcgtgccgt ggctgtaccg ttttgaactc atgggtacgc
     2521 aaagcgatat cgaaaacgtt cgtaaaggag taaccgaatt ttggcaacga caaccgcaga
     2581 atgcctgacg caggaaacga tggattacca cgcgctgaat gcgatgctta acctctacga
     2641 tagcgcaggt cgcattcagt tcgataaaga ccgccaggcc gttgacgcct ttatggcgac
     2701 gcatgtgcgt ccgaacagtg tgaccttcag tagccagcag cagcgcctga actggctggt
     2761 caacgaaggt tactatgatg aaagcgttct taatcgctac tctcgcgact ttgtcattac
     2821 gctgtttgcc cacgcacaca ccagcggttt tcgtttccag acattcctcg gggcatggaa
     2881 gttttacacc agctatacgt tgaagacatt cgacggtaaa cgttatctgg aagattttgc
     2941 cgatcgagtc acgatggtgg cgctgacgct ggcacaaggc gatgcgacgc tggcgttgca
     3001 actgaccgat gaaatgctgt caggacgctt tcagccagcc acgccgacat tcctcaactg
     3061 cggtaagcag cagcgcggcg aactggtttc ctgttttctg ctgcgtattg aagacaatat
     3121 ggagtcgatt ggccgggcgg taaattccgc actgcaactg tcgaaacgcg gcggcggcgt
     3181 ggcatttttg ctgtcgaatc tgcgagaagc gggcgcgcca attaaacgta ttgaaaatca
     3241 atcttctggc gtaattccgg tgatgaaaat gctggaagac gcattttcct atgccaacca
     3301 actcggcgcg cgtcaggggg caggtgcagt ctatttacat gctcatcatc ccgatattct
     3361 gcgttttctc gatacaaaac gggaaaatgc cgacgaaaaa atccgcatta aaacactgtc
     3421 gctgggcgtg gtgatcccgg atatcacttt ccatctggca aaagagaatg cgcagatggc
     3481 gctgttttcg ccctatgacg ttgagcgagt ttatggcaag ccgtttgccg atatcgccat
     3541 cagcgaacac tatgacgaac tggttgccga tgaacgcatc cgcaaaaaat acctcaacgc
     3601 ccgtgatttc ttccagcggc tggcagaaat ccagtttgag tccggctatc cctacatcat
     3661 gtatgaagac acggtaaacc gtgctaaccc tgtcgccggg cgcataaata tgagtaatct
     3721 ctgctcagag attttgcagg tcaactgtgc ctcagagtat gacgagaatc tcgactatgc
     3781 ccgcacaggc catgatatct cctgcaattt aggttcgttg aatattgcgc acaccatgga
     3841 ttcccccgat tttgcccgca cggtagagac tgccgtgcgc ggtttaacgg cggtgtcaga
     3901 tatgagtcat atccgcagcg tgcagtccat cgaagccgga aatgccgcct cacacgccat
     3961 cggactgggg cagatgaatt tacacggcta tctggcgcga gaaggcatcg cttatggttc
     4021 gccggaagca ctggatttca ccaatctcta tttctatgcc atcacctggc acgcactgcg
     4081 tacctcgatg ttgctggcgc gcgaacgcgg tgaaaccttc gccgggttca aacagtcacg
     4141 ttatgccagt ggtgaatatt ttagccaata tctgcaaggt aactggcagc cgaaaacggc
     4201 gaaagttggc gaactgtttg cccgcagcgg tattacgtta cctacccgtg agatgtgggc
     4261 gcaactgcgc gacgacgtga tgcgctacgg catttacaac cagaatcttc aggcggtgcc
     4321 gccaaccggt tctatctctt atatcaacca tgctacgtcg agtattcatc cgattgtggc
     4381 gaaagtagag atacgcaaag agggcaaaac aggacgcgtt tactaccctg ccccgtttat
     4441 gactaacgaa aatctggcgc tgtatcagga tgcttacgaa atcggcgcag aaaagatcat
     4501 cgacacctac gcggaagcga ctcgccatgt cgatcagggt ttgtcgctga cgctgttttt
     4561 ccccgatacc gccaccactc gcgatatcaa caaagcgcag atttacgcct ggcgcaaagg
     4621 tatcaaaacg ctctattaca ttcgcctgcg tcagatggcg ctggaaggca ctgaaattga
     4681 aggctgcgtc tcctgtgcac tttaaggaat atctatgaaa ctctcacgta tcagcgccat
     4741 caactggaac aagatatctg acgataaaga tctggaggtg tggaatcgcc tgaccagcaa
     4801 tttctggcta ccagaaaagg tgccgctgtc gaacgatatt cctgcctggc agacattaac
     4861 tgtcgtagaa caacaactga cgatgcgcgt ttttactggc ctgacgctgc tcgacacgct
     4921 gcaaaatgtt atcggcgcgc cttcgctgat gcccgatgca ctcacgcctc atgaagaagc
     4981 ggtattatcg aatatcagct ttatggaagc ggttcatgcc cgctcttaca gttcgatttt
     5041 ctcgacgctg tgccagacca aagatgtcga tgccgcctac gcctggagtg aagaaaacgc
     5101 accgttgcag cgaaaagctc agattattca gcaacattat cgcggtgatg atccgctgaa
     5161 aaagaaaatc gccagtgtgt ttcttgaatc ttttttgttc tattccggtt tctggctgcc
     5221 gatgtatttt tccagccgcg gaaagctgac caataccgca gacctgatcc gtctgattat
     5281 ccgtgatgaa gcagtccacg gttactacat aggctataaa tatcagaaaa acatggaaaa
     5341 gatatctctg ggacaacgtg aagagttgaa gagtttcgcc ttcgatttgt tgctggaact
     5401 ctacgacaac gagctgcaat acaccgatga gctgtacgcc gaaaccccgt gggctgacga
     5461 tgtgaaagcg tttctctgtt acaacgccaa taaggctttg atgaatctgg gctacgaacc
     5521 tttatttccc gcagaaatgg cggaagtgaa tccggcaatc ctcgccgcgc tttcgccgaa
     5581 tgccgatgaa aatcacgatt tcttttccgg ttcaggctcc tcttatgtga tggggaaagc
     5641 ggttgaaaca gaagatgaag actggaattt ctgagggtgt tattttcaaa aatatcacta
     5701 cccgcgacag ggaaataatt cccgccaaat agctttttat cacgcaaata ttttgtggtg
     5761 atctacactg atactctgtt gcattattcg cctgaaacca caatattcag gcgttttttc
     5821 gctatctttg acaaaaaata tcaacttttc tcgatttgct ctcagccctt atatcacggg
     5881 aaattccggc gatttgctcg catcaatatt catgccacat ttgccatcag gggttgcctc
     5941 agattctcag tatgttaggg tagaaaaaag taactatttc cattgggtaa tatatcgaca
     6001 taaacaaata acaggaatct ttctattgca tggcaattaa attagaaatt aaaaaccttt
     6061 ataaaatatt tggtgagcat ccacagcgag cattcaaata tatcgaacaa ggactttcaa
     6121 aagaacaaat tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca
     6181 ttgaagaagg cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg
     6241 tacgccttct caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg
     6301 atattgccaa aatatccgat gccaaactcc gtgaggtgcg cagaaaaaag attgcgatgg
     6361 tcttccagtc ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta
     6421 tggaattggc cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc
     6481 aggtcgggct ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc
     6541 aacgtgtggg attagcccgc gcgttagcga ttaatccgga tattttatta atggacgaag
     6601 ccttctcggc gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac
     6661 aggcgaaaca tcagcgcacc attgtcttta tttcccacga tctcgatgaa gccatgcgta
     6721 ttggcgaccg aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg
     6781 aaattctcaa taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta
     6841 gtcaggtatt cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa
     6901 cccctggctt cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg
     6961 gctacgttat cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa
     7021 ccgcgttaac gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg
     7081 atgcacaaac gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc
     7141 ccgtggtcga cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg
     7201 ctttagatcg tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca
     7261 gcggcggaca gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccgact
     7321 gacggcggtg gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat
     7381 attctcgatc cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc
     7441 gactgggttg ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat
     7501 atcctcaaca gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt
     7561 ttcgctctca tcgcctggca gatttccggg gtcggaatgg gcgtggcgac gctggtttcg
     7621 ctgattgcca tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg
     7681 ttaaccgccc tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga
     7741 agtccgcgag cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg
     7801 tttgtttatc tggtaccaat cgtcatgctg tttggtatcg gtaacgtgcc gggcgtagtg
     7861 gtgacgatca tctttgctct gccgccgatt atccgtctga cgattctggg gattaaccag
     7921 gttccggcgg atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg
     7981 ttcaaagttc agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg
     8041 atgctggctc tttctatggt ggtcatcgcc tcgatgattg ccgtcggcgg gctgggtcag
     8101 atggtacttc gcggtatcgg tcgtctggat atgggccttg ccaccgttgg cggcgtcggg
     8161 attgtgatcc tcgccattat cctcgaccgc ctgacgcagg ccgttgggcg cgactcacgc
     8221 agtcgcggca accgtcgctg gtacaccact ggccctgtcg gtctgctgac ccgcccattc
     8281 attaagtaac tctgcacttg cccggtaacg ccgggcatta tcaccctgcc aaaaaaagga
     8341 ataacaatgc gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa
     8401 acttttgcag ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc
     8461 actgaagaaa ccttccagac gctgctggtc agtcgtgcac tggagaaatt aggttatacc
     8521 gtcaataaac ccagcgaagt ggattacaac gttggctaca cctcacttgc ttccggcgat
     8581 gcaaccttca ccgccgtgaa ctggacgccg ctgcatgaca acatgtacga agctgccggt
     8641 ggcgataaga aattttattg tgaaggggta ttcgttaacg gcgcggcaca gggttacctg
     8701 atcgataaga aaaccgccga ccagtacaaa atcaccaaca tcgcacaact gaaagatccg
     8761 aagatcgcca aactgttcga taccaacggc gacggtaaag cggatttaac cggctgtaac
     8821 ccaggctggg gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac
     8881 accgtgacgc ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac
     8941 aaagagggca aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg
     9001 aagccaggga aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa
     9061 aacgccgata ccaaactgcc gaatggtgcg aattatggct tcccggtcag caccatgcat
     9121 atcgttgcca acaaagcctg ggccgagaaa aacccggcag cagcgaaact ttttgccatt
     9181 atgcagttgc cagtggcaga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc
     9241 tcagaaggcg atattcaggg ccatgttgat ggttggatca aagcccacca gcagcagttc
     9301 gatggctggg tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagt
     9361 cgtttttgcc tcatccgcca ttctgtgcac aatgcctgat gcgacgctgg cgcgtcttat
     9421 catgcctaca aaatacactc attccgtatg gcggataagg cgttttcgcc gcatccgccg
     9481 ttctgtgcac aatgcctgat gcgacgctgg cgcgtcttat catgcctaca aaccacatcg
     9541 cacatttcaa caagctatct ttcgccccca ttcatcaaca tccgctatta ttgatttcca
     9601 gcttaatcat cacctgatga acaaaaataa tgactaaacc taatcatgag cttagcccgg
     9661 cgctgatcgt gctgatgtct atcgccaccg gtcaggcggt agccagtaac tattacgccc
     9721 agccattgct cgacaccatc gcgcgtaact tttccctttc cgccagttcg gcaggcttta
     9781 ttgttaccgc cgcgcagttg ggctatgcag caggtctgct gtttcttgtt cccctcggtg
     9841 atatgtttga acgccgccgc ctgattgtct cgatgacctt actggcggca ggcggcatgt
     9901 taatcactgc cagcagtcag tcgctggcga tgatgatcct cggtacggca ttaaccggtt
     9961 tattctcagt cgtggcacaa attctggttc cgctggcagc gacgctggct tcaccggata
    10021 aacgcggcaa agtggttggc accattatga gcggtctgct gttggggatc ttgctggcac
    10081 gaaccgttgc cgggttactg gcaaacctcg gcggctggcg caccgtcttt tgggttgcct
    10141 cggtgttaat ggcactgatg gcgctagcat tatggcgtgg tctgccacaa atgaaatcag
    10201 aaacccacct caactaccca cagttgttag gttccgtttt cagcatgttt atcagcgata
    10261 agatcctccg cacccgcgca ttgctgggct gcctgacctt tgccaacttc agcattctct
    10321 ggacctcaat ggcctttttg cttgccgctc caccttttaa ctacagcgat ggtgtcattg
    10381 gtctgtttgg acttgcggga gctgccggag cgttgggcgc tcgtccggcg ggcggttttg
    10441 ccgataaggg taaatcgcac cacaccacaa ctttcggtct gctgctgcta ttactttcat
    10501 ggctggcgat ctggtttggt cacacttccg tactggcgct gattatcggc atcctggtgc
    10561 tggatctcac cgtacagggc gtgcatatca ctaaccagac ggtaatttat cgaatacacc
    10621 ctgatgcgcg taatcgcctg accgcaggtt acatgaccag ctactttatt ggcggtgccg
    10681 ccggttcgct aatttcagcc tcagcctggc aacatggcgg ttgggctggc gtttgtctgg
    10741 ctggcgcgac gattgcccta gttaacttac tggtctggtg gcgaggtttt catcgtcagg
    10801 aagccgcaaa ttaagcaatc agcaaccgtt ttcgtggctt tacacttata agggtgttaa
    10861 gaagcccatc agtctgataa ggttaagata ttcattcagt ctatttataa tattaacaat
    10921 cgttaagcgt acactctatg gaaagcccta ctccacagcc tgctcctggt tcggcgacct
    10981 tcatggaagg atgcaaagac agtttaccga ttgttattag ttatattccg gtggcctttg
    11041 cgttcggtct gaatgcgacc cgtctgggat tctctcctct cgaaagcgtt tttttctcct
    11101 gcatcattta tgcaggcgcg agccagttcg tcattaccgc gatgctggca gccgggagta
    11161 gtttgtgggt tgctgcactg accgtcatgg caatggatgt tcgccatgtg ttgtatggcc
    11221 cgtcactgcg tagccgtatt attcagcgtc tgcaaaaatc gaaaaccgcc ctgtgggcgt
    11281 ttggcctgac ggatgaggtt tttgccgccg caacagcaaa actggtacgc aataatcgcc
    11341 gctggagcga gaactggatg atcggcattg ccttcagttc atggtcatcg tgggtatttg
    11401 gtacggtaat aggggcattc tccggcagcg gcttgctgca aggttatccc gccgttgaag
    11461 cagcattagg ttttatgctt ccggcactct ttatgagttt cctgctcgcc tctttccagc
    11521 gcaaacaatc tctttgcgtt accgcagcgt tagttggtgc ccttgcaggc gtaacgctat
    11581 tttctattcc cgtcgccatt ctggcaggca ttgtctgtgg ctgcctcact gcgttaatcc
    11641 aggcattctg gcaaggagcg cccgatgagc tatgaggttc tgctgcttgg gttactggtt
    11701 ggcgcggcga attattgctt ccgctatttg ccgctgcgcc tgcgtgtggg taatgcccgc
    11761 ccaaccaaac gtggcgcggt aggtattttg ctcgacacca ttggcatcgc ctcgatatgc
    11821 gctctgctgg ttgtctctac cgcaccagaa gtgatgcacg atacacgccg tttcgtgccc
    11881 acgctggtcg gcttcgcggt actgggtgcc agtttctata aaacacgcag cattatcatc
    11941 ccaacactgc ttagtgcgct ggcctatggg ctcgcctgga aagtgatggc gattatataa
    12001 cccacaagaa tcatttttct aaaacaatac atttacttta tttgtcactg tcgttactat
    12061 atcggctgaa attaatgagg tcatacccaa atggatagtt cgtttacgcc cattgaacaa
    12121 atgctaaaat ttcgcgccag ccgccacgaa gattttcctt atcaggagat ccttctgact
    12181 cgtctttgca tgcacatgca aagcaagctg ctggagaacc gcaataaaat gctgaaggct
    12241 caggggatta acgagacgtt gtttatggcg ttgattacgc tggagtctca ggaaaaccac
    12301 agtattcagc cttctgaatt aagttgtgct cttggatcat cccgtaccaa cgcgacgcgt
    12361 attgccgatg aactggaaaa acgcggttgg atcgaacgtc gtgaaagcga taacgatcgc
    12421 cgctgcctgc atctgcaatt aacggaaaaa ggtcacgagt ttttgcgcga ggttttacca
    12481 ccgcagcata actgcctgca tcaactctgg tccgcgctca gcacaacaga aaaagatcag
    12541 ctcgagcaaa tcacccgcaa attgctctcc cgtctcgacc agatggaaca agacggtgtg
    12601 gttctcgaag cgatgagcta acgcgtcatc tcgctcaaaa atccagattt ataaaagaaa
    12661 aaatgaccgg ccagcatcgc aacatgctgg cctttttggc aagcaggtcg gctcagccga
    12721 tgagttaaga agatcgtgga gaacaatatg agcgcaaatg cggagactca aaccccgcag
    12781 caaccggtaa agaagagcgg caaacgtaag cgtctgctcc tccttctcac cttgctcttt
    12841 ataattattg ccgtagcgat agggatttat tgttttttgg tactgcgtca tttcgaagaa
    12901 accgatgacg catacgtggc agggaatcag attcaaatta tgtctcaggt gtctggcagc
    12961 gtgacgaaag tctgggccga taacaccgat tttgtaaaag aaggcgacgt gctggtcact
    13021 ctcgacccga cagatgctcg ccaggcgttt gaaaaagcca aaactgcact ggcttccagc
    13081 gttcgccaaa cccaccagct gatgattaac agcaaacagt tgcaggcgaa tattgaggtg
    13141 cagaaaatcg ccctcgcaaa agcacaaagc gactacaacc gccgtgtgcc gctgggcaat
    13201 gccaacctga ttggtcgcga agagctgcaa cacgcccgcg acgccgtcac cagtgcccag
    13261 gcgcaactgg acgtcgctat tcaacaatac aatgccaatc aggcgatgat tctggggact
    13321 aaactggaag atcagcctgc cgtgcaacag gctgccaccg aagtacgtaa cgcctggctg
    13381 gcgctggagc gtacccgtat tgtcagtccg atgaccggtt atgtctcccg ccgcgcggta
    13441 cagcctgggg cgcaaattag cccaacgacg ccgctgatgg cggtcgttcc agccaccaat
    13501 atgtgggtgg atgccaactt taaagagacg cagattgcca atatgcgtat cggtcagccg
    13561 gtcactatca ccacggatat ttacggcgat gatgtgaaat acaccggtaa agtggttggt
    13621 ctggatatgg gcacaggtag cgcattctca ctgcttccgg cgcaaaatgc gaccggtaac
    13681 tggatcaaag tcgttcagcg tctacctgtg cgtatcgaac tggaccagaa acagctggag
    13741 caatacccgc tgcgcattgg tttgtccaca ctggtgagcg tcaataccac taaccgtgac
    13801 ggtcaggtac tggcaaataa agtacgttca acaccggtcg cggtaagtac cgcgcgtgaa
    13861 atcagcctgg cacctgtcaa taaactgatc gacgatatcg taaaagctaa cgctggctaa
    13921 tccagaggtg cgtgtgatgc aacagcaaaa accgctggaa ggcgcgcaac tggtcattat
    13981 gacgattgcg ctgtcactgg cgacattcat gcaggtgctg gactccacca ttgctaacgt
    14041 ggcgatcccc actatcgccg ggaatctggg ctcatcgctc agccagggaa cgtgggtaat
    14101 cacttctttc ggggtggcga atgccatctc gatcccgctt accggctggc tggcaaagcg
    14161 cgtcggggaa gtgaaactgt tcctttggtc caccatcgcc tttgctattg cgtcgtgggc
    14221 gtgtggtgtc tccagcagcc tgaatatgct gatcttcttc cgcgtgattc aggggattgt
    14281 cgccgggccg ttgatcccgc tttcgcaaag tctattgctg aataactacc cgccagccaa
    14341 acgctcgatc gcgctggcgt tgtggtcgat gacggtgatt gtcgcgccaa tttgcggccc
    14401 gatcctcggc ggttatatca gcgataatta ccactggggc tggatattct tcatcaacgt
    14461 gccgattggc gtggcggtgg tgttgatgac actgcaaact ctgcgcggac gtgaaacccg
    14521 caccgaacgg cggcggattg atgccgtggg gctggcactg ctggttattg gtatcggcag
    14581 cctgcagatt atgctcgacc gcggtaaaga gctggactgg ttttcatcac aggaaattat
    14641 catccttacc gtggtggcgg tggtggctat ctgcttcctg attgtctggg agctgaccga
    14701 cgataacccg atagtcgatc tgtcgttgtt taagtcgcgc aacttcacca tcggctgctt
    14761 gtgtatcagc ctcgcgtata tgctctactt cggcgctatt gttctgctgc cgcagttgtt
    14821 gcaggaggtc tacggttaca cggcgacctg ggcaggtttg gcctctgcgc cggtagggat
    14881 tattccggtg atcctgtcgc cgattatcgg ccgcttcgcg cataaactgg atatgcggcg
    14941 gctggtaacc ttcagcttta ttatgtatgc cgtctgcttc tactggcgtg cctatacctt
    15001 tgaaccaggt atggattttg gcgcgtcggc ctggccgcag tttatccagg ggtttgcggt
    15061 ggcctgcttc tttatgccgc tgaccaccat tacgctgtct ggtttgccac cggaacgact
    15121 ggcggcggca tcgagcctct ctaactttac gcgaacgctg gcggggtcta tcggcacgtc
    15181 gataaccacg accatgtgga ccaaccgcga gtcgatgcac catgcgcagt tgactgagtc
    15241 ggtaaacccg ttcaacccga atgcccaggc gatgtacagt caactggaag ggcttgggat
    15301 gacgcaacag caggcatcag gctggattgc ccagcagatc accaatcagg ggctgattat
    15361 ttccgccaat gagatcttct ggatgtcagc cgggatattc ctcgtactgc tggggctggt
    15421 gtggtttgct aaaccgccat ttggcgcagg tggcggtggc ggcggtgcgc actaagtaca
    15481 actaagccag ttcatttgaa ctggcttttt tcaattaatt gtgaagatag tttactgact
    15541 agatgtgcag ttcctgcaac ttctctttcg gcagtgccag ttcttcgttg ctgttgatgc
    15601 gtacgtcacg ttccagaatg ctacgcgcaa tatcctgcgc ttcctgcaac gagtgcatct
    15661 ggtaagtgcc acactggtag acgttcagtt ccgggatctg attctgatcc tgcactttca
    15721 gcacgtcttc cattgccgct ttccaggcat cagcaacacg ctgctcatct ggcgtaccaa
    15781 tcagactcat ataaaaaccg gtgcggcagc ccattggcga gatatcgata atctctacac
    15841 cattaccgtt aagatggtta cgcataaaac cagcaaacag gtgctccagg gtatggatcc
    15901 ctctttctgg catcacttct ttgttcggca cgcagaagcg cagatcgaac acggtgattg
    15961 cgtcgccatg cggggtgttc attgttttcg ccacccgaac tgcaggcgct tccatccggg
    16021 tatgatcgac tgtgaagcta tctaacaacg gcatttagcc acctccggta atttttttaa
    16081 aaattttctg aactctttct tcccaggcga gtctgagtat atgaaagacg cgcatttgtt
    16141 atcatcatcc ctgaattcag agatgaaatt ttggccactc acgagtggcc tttttctttt
    16201 ctgtcaggtg tgtttttcca gccacaccgc aaacggttcg gtatcagcgg tttccatttc
    16261 ctgctgacgg cgttcagatg cctcgcgctc ggctacaaaa tcctcttcgc gcagaatttc
    16321 cagcggctct tcacgcagca gattacggta ggcttctgca aacgctttgc ctgttccgcc
    16381 aataccagta tcaatcatag accttaagat acgggcagag aaagtcagat cgggattatc
    16441 gaagcaggca accagttcat cacacacttt ctgatacgcg tcgccgccgt taatgctatc
    16501 cagcgtttgc gcgacgcgtt tcagatcgcg gaacagatct ttacccacct gcagtaacgg
    16561 gaactgcgcg gtttcgcagc cgatacccag cgtcaggccc ggtttgcgac cttcaaggat
    16621 cacccggttc cagttaacgc gcgtacaggc aagttcgcta ctgctcattt ccggtgcatc
    16681 agccagcgca caccagacca taaacaggtc gaggaatcgc acctgctgtt catctacacc
    16741 aatcggcgag aacgggttga tgtccagcga acgcacttca atatattcaa tgccgccacg
    16801 taacagcgca tcagaaggtg actcgccgct gcgggtaacg cgttttggac gaatcggcgc
    16861 atacagttcg ttttcaatct gcagcacgtt gctattgatt tgcagcctct taccgtcttt
    16921 ctcaatacca atcttcgcgt actcttccga tggcgttttg attgcctgtt taaggcccgc
    16981 tacgtactcg taaagatcgt tgaaggtaat accaagattg ctttgcgatt tattggtata
    17041 gccgagatcg ctcaaacgaa gagaggtcgc atacggcagg taatacatac cgcactcggt
    17101 tttctcaaac ggcagcgacg ttggttttcc ttgcaggaaa gaagaacaaa tcgccggaga
    17161 tgcaccaaac agataaggaa tgacccaacc gaaacgatag taattgcgga taacgcggaa
    17221 atagcccgca gaaattttct ctttggcatc agcgcccgag atatcaccgc acttcgcttg
    17281 ccagaatgcc attggcaaag agaaattgta gtgcacgccg gaaatggttt gcatcagcgc
    17341 gccgtagcga tttttcagcc cttcacgata cagcgtttta aagcgtccgg tgttagaagt
    17401 gccgtactgt gccagttcga tgtcctgacc ttctgcgatg tagcatggca tacttaacgg
    17461 ccacatccgc tcatcgccca tattgcgcgc cgtataacga tgcagatcgc gcataaaggt
    17521 cagcatatgt tcaatatcac catccactgg tgtaatgaat tccagcaatg cttccgcaaa
    17581 atcggtagta atccatttgt gcgtcagtgc ggaacctaat gcttcaggat gacctgttgt
    17641 tgccagtgtg ccatcagcat taacacgcaa agtttcgcgc tccagcccac gctgtatccc
    17701 ctttaacgcc tgaggatgtt tttccagcca ggccagcgcc tgtgatacgt ccgggatcaa
    17761 attgacctcc cgcctgtcaa aatcgtttta attagcataa ctgtaatggt gaccatatgt
    17821 gcaggcctac aattagtgcc accacatcat gccctgaacg gtcgctgctg caactgcaac
    17881 atagcgtaac gctttaccaa ggcacaaaaa aaagattacc ggtccccacg agatgcgcat
    17941 ccatcccgct aacagacaca gtaaatcgcc aaccacgggc atccagctta ataatagcgt
    18001 gactgcacca tagcgtttca gccagccgat agctttctct tgccagcgcg atgttttacg
    18061 caatggaaag aaacgcccaa ggataacgtt agttaaccct ccaaggctat tacccattgt
    18121 tgctgttaaa actaaaaccc agggatgact gatcccggaa agcaacattg ccaccagcac
    18181 gacttcggag ttgccgggta atagtgtagc gctgagaaaa ctactggcaa acaacgagaa
    18241 aagcgataac gcttcactca cagcaagcga acgtccacag cgtccatacc tgcggcacgg
    18301 gccgcctgaa taccgaaatc ggcatcttca aagaccacac actgcgtcgg ttgcacgccc
    18361 atacgctgcg cgcacaacaa aaatgtgtct ggcgcgggtt tatggtgttt gacgtgatcg
    18421 gcagcgacga cggcgtcaaa ataatggcgt aatcccaggt gcgccagcaa tgcctcagcg
    18481 atggcgcttt cactccccgt tcctacagcc attgggcgac gaccatgcca acttttcacc
    18541 acatcaacaa gaggaagcgg ttcgacgcta tccagcagca tacttcttac tgcttctgtt
    18601 ttttcacgcg ctaacgcatg cgggtcgaga tcggcctgat tcagctcaat aattgcctga
    18661 gcaatacgcc aggtgggcga tccattaagc gcaatcatcg cctgaatatc gtactgaaga
    18721 ccgtagtgcc ctaatacttc gcgccacgct ttacggtgcg taggctccgt atccaggatt
    18781 gtgccatcca tatcaaaaat taaacctgca taacgctcgt acatggtctt ctcgcaagtc
    18841 gaaaaatatg acgctacttt agcgtaattg cttgattttg tcgctgatgg cgaaggagaa
    18901 gaaaattcag gaaatggata aaggagttat ttagaaatga gatatttttg aaggaaattt
    18961 tttggagaag atggtgcatc cgggaggatg actcactgac gttcgccctt cgggccgttg
    19021 ctggcgcaac gttatccttc ctggtgcctg cggttaatac cgctatctct tcgatacctt
    19081 cattgctgaa ggttacggag aatatggtgc atccgggagg attcgaacct ccgaccgctc
    19141 ggttcgtagc cgagtactct atccagctga gctacggatg catcgggaaa acttattcta
    19201 ctgctgatat tgataccgct actaaagcca tatcaattaa gagatggtgc atccgggagg
    19261 attactcgcc ttgcggctcg cccttcgggc cgttgctaaa gcaacgttat catccctggt
    19321 gcctgcggtt aattccgcta cctcttcgat accttcattg ctgaagattt cggagaatat
    19381 ggtgcatccg ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca
    19441 gctgagctac ggatgcatcg ggaaaattta ttttactgct gatattgata ccgctactaa
    19501 agccatatca agtaagagat ggtgcatccg ggaggattcg aacctccgac cgctcggttc
    19561 gtagccgagt actctatcca gctgagctac ggatgcaaat ggcggtgagg cggggattcg
    19621 aaccccggat gcagcttttg accgcatact cccttagcag gggagcgcct tcagcctctc
    19681 ggccacctca ccacacgcct cttacgagtg cttcgaagaa cttgtttatc gctcatcgtc
    19741 gctgcgtggc gcacatatta ctttctgaga cttataagtc aaacaatttt tcccacactt
    19801 ttatcgtttg cacacttcac gctcaattag tctgtaaaaa cgtcaaaaag agtgttttat
    19861 ctacagaaaa acggaggtct ggcagatagc agtaatgcaa aaaaatggag acttaagttg
    19921 aatgaacggg agtaaagcga aaagactata gagtaaagga gaaattttga gggtgcgtct
    19981 caccgataaa gatgagacgc ggaaagatta gtaactggac tgctgggatt tttcagcctg
    20041 gatacgctgg tagatctctt cacggtgaac agaaacttcc ttcggggcat ttacgccaat
    20101 acgtacctgg ttgcccttta cccctaaaac tgtcacggtg acctcatccc caatcatgag
    20161 ggtctcacca actcgacgag tcagaatcag cattctttgc tccttgaaag attaaaagag
    20221 tcgggtctct ctgtatcccg gcattatcca tcatataacg ccaaaaagta agcaatgaca
    20281 aacacattac atgtaagcag tcatggcatt acattctgtt aaacctaagt ttagccgata
    20341 tacacaactt caacctgact ttatcgttgt cgatagcgtt aatgcgaatg ccgtgaagcg
    20401 agtccacggc attgcctgac gcttatatta ttgcaatttc gcgctgaccc agcctttcac
    20461 actggctaac gctgcaggta aggccgcagc atccgtacca ccggcttgcg ccatgtcagg
    20521 acgtccacca cccttgccgc ccacctgctg agcgaccata ccaatcagtt cccctgcttt
    20581 cacacgatct gtgacgtcct tagatacgcc tgcaatcaga gaaaccttac cttcggctac
    20641 cgttgccagc acgataattg tcgaccccag ctgatttttt aaatcgtcaa ccatggtacg
    20701 caacattttc ggctcaacac cgctaagctc gctaaccaac agcttaacac cattaacatc
    20761 aattgcctta ctggaaagat ttgcgctctc ctgtgcggca gcttgttctt taagctgttg
    20821 tagttctttt tctaactgac gcgtacgttc cagtactgag cgcactttat ccgccagatt
    20881 attgctatcg cctttcagca gatgcgcgac ttcgcttaag cgatcgctgt ctgcatgaac
    20941 ggtggtgata gcgccttctc cggttaccgc ttcgatacga cgaacgcctg cagcagtacc
    21001 cgattcagag atgatgcgga acagaccaat atcaccagtg cggctggcgt gagtaccgcc
    21061 acacagctcg gtggagaaat cgcccatgct cagcacgcgt acacgctcat catacttctc
    21121 gccgaacagc gccatcgcac ctttcgcttt cgccgcttcg agatccatga tgttggtttc
    21181 gatcggcaaa ttgcgacgaa tctgtgcgtt caccaggtct tcgaccgcac gaatctcttc
    21241 cggtttcatc gcttcgttgt gtgagaagtc gaagcgcagc accttgtcgt taaccagtga
    21301 acctttctgc gatacatgag tccccagaac ctggcgcagc gcagcgtgca tcaggtgcgt
    21361 tgcggagtga ttcagacgaa tacgggcgcg acgagcctca tcaacatcag cctgcacagc
    21421 gtcgcccact ttcagagaac ccgcagcaag tttaccgatg tgaccaatcg cctggccgta
    21481 tttctgcgta tcttccaccg cgaaggagaa gttagcgcct ttcagttcgc ctttatcacc
    21541 aacctgaccg ccggattccg catagaatgg cgtttgatcc agcacgacca cagcttcctg
    21601 gcctgcattg atggcatcaa ccgctttacc atcaacaaac agcgcggtca ctttgccgtt
    21661 cagttccaga tggtcatagc ctttaaattc agatgcacta tcaacacgga tcattgcgtt
    21721 gtaatcggca ccaaagccgc tggcttcgcg cgcgcgacga cgctgctctt ccattgctgc
    21781 ttcaaaacca gcttcgtcaa ctttgatgtt gcgctcacga caaacatcag ccgtcaggtc
    21841 aaccgggaag ccataggtgt cgtacagacg gaaagccgtt tcaccatcca gcgtatcacc
    21901 agaaagtttt gccagctctt catccagcaa cgccagaccg cgctccagag tacgggcaaa
    21961 ctgctcttct tcagtcttca gcacctgctc aacctgcgcc tgctggcgtt tcaggtcttc
    22021 acccgcagag cccataacgt cgatcagcgg accaaccagt ttgtagaaga aggtttcttt
    22081 cgcgccgagc atattgccgt gacgcactgc gcgacgaatg atacgacgca gtacataacc
    22141 acggttttca ttggacggca ttacgccatc cgcgatcagg aacgcacaag aacgaatgtg
    22201 gtcagcgatt acgcgcagcg atttattgct cagatcggtc gcgccagtga ctttcgctac
    22261 cgcctggatc aacgtgcgga acaggtcgat gtcatagtta gagttaacgt gttgcagcac
    22321 cgcagcaata cgctccagac ccataccggt atctacagac ggcttcggca gcggttccat
    22381 cgtgccatct gcctggcggt tgaactgcat gaagacgatg ttccagatct caatgtagcg
    22441 gtcgccgtct tcttccggac ttcccggagg gccaccccaa atgtggtcgc cgtgatcgta
    22501 gaagatttcg gtgcacgggc cgcacggacc agtgtcaccc atctgccaga agttgtcaga
    22561 tgcgtatggc gcacctttgt tatcgccgat gcgaataata cgttcgcgcg ggatccctac
    22621 ttctttttcc cagatttcgt aggcttcgtc gtcgctttca tagacggtaa cccacagacg
    22681 ctctttcggc agggcaaacc atttttcgct ggtcagcagt tcccatgcaa actgaatggc
    22741 atcgtgtttg aaatagtcgc cgaagctgaa gttgcccagc atttcgaaga aggtatggtg
    22801 acgcgcggtg taaccgacgt tttccaggtc gttgtgttta ccacccgcac gcacgcagcg
    22861 ttgggaagtg gtagcgcggg aataattacg cttgtcgagc ccaaggaaca catccttgaa
    22921 ctggttcatc ccggcgttgg taaacaacaa agttgggtcg ttatggggta ccagggagct
    22981 gctggcaact acctgatgtc ccttactatg gaaaaagtcg agaaacgcct gacggatctc
    23041 agcggtgctc ttgctcataa ttatcctgaa atcaagctaa cgaaatatcg ccaccagctc
    23101 cagcgtgtct taaccgccgg gctggtaact gaaaagtggg aataagataa gttttcttga
    23161 gtgggaagta aaataccgta tgcgttcagt cggcaaaatt tcgccaaata tcctggatat
    23221 cttccatcag atagccacga tagagcagaa aacgctggat cttaactttt tctgaaaaga
    23281 cagttggcaa aggttcacca tattttcgcg tcgcctgatc gcgcaccagt gcgcaccagt
    23341 cgatgtcaca ttcacgcatc gctttttctg tcgcttcgcg ggaaatacct ttctgattca
    23401 gttcctgacg aatacgtgca ggtccataac ctttgcggct acggctggcg ataaagcgcg
    23461 caacaaatcg gctgtcatcg agatagccat gttcatggca ccaggcaata acgcgctcgt
    23521 aatcttctgc cgtagcatca atctcttctg ggccattttt gcccataatc ggtgccgcga
    23581 gtttacgtcg cagttcttgc tcactgtgat cgcgcaccgc cagaatgcgt accgcacgat
    23641 ccaacaggcg agcatatgcc gggcgacggg atgttgattc tgtcatggca tatccttaca
    23701 acttaaaaaa gcaaaagggc cgcagatgcg acccaggttt tgctgataaa cagatttaat
    23761 agtcaatgag tgtgcagcca attcgttaga ggatcgaggt gagagtaatt tttcacgcca
    23821 gtgttggcct gttcccctca ccctaaccct ctccccaaag gggcgagggg accgtccgtg
    23881 cacacattat ctattatcat caataacgaa cgattaaaaa tcttcgttag tttctgctac
    23941 gccttcgctg tcatctacag agaaatccgg cgttgagttc gggttgctca gcagcaactc
    24001 acgtactttc ttctcgatct ctttcgcggt ttccgggtta tctttcagcc aggcagttgc
    24061 attcgcttta ccctgaccga tcttctcacc tttgtagctg taccacgcgc ctgctttctc
    24121 gatcagcttc tcttttacgc ccaggtcaac cagttcgccg tagaagttga taccttcgcc
    24181 gtagaggatc tgaaattcag cctgtttaaa cggtgcagcg attttgttct tcaccacttt
    24241 cacgcgggtt tcgctaccca ccacgttttc gccctctttc accgcgccga tacgacggat
    24301 gtcgagacga acagaggcgt agaatttcag cgcgttacca ccggtagtgg tttccgggtt
    24361 accgaacatc acaccaattt tcatacggat ctggttgatg aagatcagca gcgtgttgga
    24421 ctgcttcagg ttacccgcca gcttacgcat cgcctggctc atcatacgtg ccgcaaggcc
    24481 catgtgagag tcgccgattt cgccttcgat ttccgctttc ggcgtcagtg ccgccacgga
    24541 gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg gcgtcacaga tttccagtgc
    24601 ctgctcgccg gtgtccggct gggagcacag caggttatcg atatcgacgc ccagtttacg
    24661 tgcgtagatt gggtccagcg cgtgttcagc atcgataaac gcacaggttt taccttcacg
    24721 ctgcgctgcg gcgatcacct gcagcgtcag cgtggtttta ccggaagatt ccggtccgta
    24781 gatttcgacg atacggccca tcggcagacc acctgcccca agcgcgatat ccagtgaaag
    24841 cgaaccggta gagatggttt ccacatccat ggaacggtct tcacccaggc gcatgatgga
    24901 gcctttacca aattgtttct caatctggcc cagtgctgcc gccaacgctt tctgtttgtt
    24961 ttcgtcgata gccattttta ctcctgtcat gccgggtaat accggatagt caatatgttc
    25021 tgttgaagca attatactgt atactcatac agtatcaagt gttttgtaga aattgttgcc
    25081 acaaggtctg caatgcatac gcagtagcct gacgacgcac cgcatcacgg tcgccgctga
    25141 agcattcccg ccgggtaatg ccttcaccgc gggcagtggc aaaagcaaac cagacggtgc
    25201 cgacaggctt ctcttcactg ccgccatccg gcccggcgat accactaata gacacggcat
    25261 aatcagcacg agccgctttc agtgcaccta tcgccatttc caccacgacg ggttcactca
    25321 ccgcgccatg ctgcgccagc gtctcttcgc gtacgccgat catctgcgct ttggcttcgt
    25381 tactgtaggt gacaaatccg cgttcaaacc aggcggagct accggcaata tcggtaatca
    25441 ctttcgctac ccaaccaccg gtacaagact cggcagttgt tacggttgcg ccacgggctt
    25501 tcagcgcctg cccaacctgt tcgcttaact gcatcagttc actgtcagtc atcacaacct
    25561 cagtcagtca aaaatttatg ccggacaaga tagcactttc gtcaggaaga tggtgttggc
    25621 gttgcaattt tacgaggagg attcagaaaa aagctgatta gccagaggga agctcacgcc
    25681 cccctcttgc aaatagttac tgcacgcgcg ccagcgccac ggcttgtcct aactgccaga
    25741 ccgccattgc gtaatgggtg ctgtggttat aacgggtaat ggtgtagaag ttcggcagac
    25801 cgtaccagta ctggtaaccg gtaccgacat ccagacgcag caggctggct tgttgatggt
    25861 tgcccagcgg ctgctgtggc gttaaacctg ccgtggcaag ctgcgaaatg ctgtacctgg
    25921 ttttgaagcc atttggcaag cctggagcct gaccgtttgc cattaccgcg acctgatcgc
    25981 ctttcaccca gccgtgcgct ttgaaatagt tcgccacact accgatcgca tcaaccggat
    26041 cccacaggtt gatatgcccg tcgccgctga aatctaccgc atattgtttg taagacgacg
    26101 gcataaactg tccgtagccc atcgccccgg caaaggaacc tttcagattg agcggatcgt
    26161 cctgctcgtc gcgcgccatc aacaggaagg tttccagctc gccagagaaa tactccgcgc
    26221 ggcgtgggta gttaaatgac agcgttgcca gcgcatcgag gatgcgagtt ttccccatca
    26281 cgcgccccca gcgggtttca acgccgataa tcccgacgat aatttccggc ggtacgccat
    26341 acacctgcca cgcgcgattc aacgcatctt catactgatt ccagaaaacc acaccgttct
    26401 gcacgttgtc cggcgtaata aattttttgc gataacgcag ccatgcgccg ttcggacctg
    26461 atggcggttt caccgatgtg gttggtgcct ggttatccat cagccgcagt accgaatcca
    26521 gacgcttcgc ctgggagaga atttcctgca actgctgacg atcgaaaccg tgtttgttca
    26581 ccattctgtc gatgaactgc tgggcattcg ggttattagc gaaatcgccg cccatctgca
    26641 tcacattgtg ctgaggctca agcaggaagc cgccagacgg cgttccggtg gtcgtttcag
    26701 tctcagtagg ttttggcttg ctgctacagg cggcaagcaa cacaaaaagg ggaagcaatg
    26761 ttacataacg acgcttgaac atgaggggtc catttaacag attcaaccag gggcaagtat
    26821 ggtaaagcat cacgccccgc acaaggaagc ggtagtcact gcccgatacg gactttacat
    26881 aactcaactc attcccctcg ctatcctttt attcaaactt tcaaattaaa atatttatct
    26941 ttcattttgc gatcaaaata acacttttaa atctttcaat ctgattagat taggttgccg
    27001 tttggtaata aaacaataaa tcctgaagga gagaacaatg atagaaacca ttactcatgg
    27061 tgcagagtgg tttatcgggc tgttccaaaa gggcggagag gtgtttaccg ggatggtgac
    27121 cggcattctt ccgctgttga ttagcctgct ggttatcatg aacgcactga ttaattttat
    27181 cggtcagcat cgtattgaac gttttgctca acgttgcgcc ggtaaccctg tttcccgtta
    27241 cctgctgcta ccgtgcattg gcacgtttgt cttttgcaat ccgatgacct taagcctggg
    27301 tcgctttatg ccggaaaagt acaaacccag ctactacgcg gcagcctctt atagctgcca
    27361 ctcaatgaat ggcctctttc cccatatcaa ccctggcgaa ctgtttgttt atcttggcat
    27421 tgccagcggt ctgacaacgc tgaacctgcc acttggccca ctggcggtga gctatctgct
    27481 ggttggtctg gtcaccaact tctttcgcgg ctgggtgacc gatctgacca ccgccatttt
    27541 tgagaaaaag atgggcattc aacttgaaca aaaagttcac ctggcaggag caacatcatg
    27601 acgcgtattc ggatcgaaaa aggaacgggt ggctggggcg gcccgcttga gctggaagcc
    27661 acgccgggca aaaaaatcgt ctatatcacc gccggtaccc ggcctgcgat tgttgacaaa
    27721 ctggcacaac ttactggctg gcaggctatt gacggattta aagaaggtga acccgcggag
    27781 gcggaaattg gtgtcgcggt aatcgactgt ggcggcacat tacgctgcgg catctatccg
    27841 aaacgacgta ttcccaccat taatatccac tcgacgggca agtccagtcc gctggcgcag
    27901 tacattgtgg aagatattta tgtctctggc gtaaaagaag aaaacatcac tgtagtgggt
    27961 gatgcgacac cacaaccctc ttccgtgggc cgtgactatg acaccagcaa gaaaatcacc
    28021 gaacaaagcg atggtttact ggcgaaggtg ggaatgggta tgggttctgc cgttgccgtg
    28081 ttgtttcaat ctggtcgtga caccatcgac actgtattaa aaaccattct gccgtttatg
    28141 gcgttcgtct cggcgctcat tggcatcatt atggcttctg gccttggtga ctggattgcc
    28201 cacggtcttg ctccgctggc gagccatcca ctgggtctgg tcatgctggc gctcatctgc
    28261 tccttcccgc tgctttcacc tttcctcggc ccaggcgcag ttatcgcaca ggttatcggc
    28321 gtattgattg gcgtgcagat tggtctcggc aatattccgc cgcatctggc tttaccggca
    28381 ctgtttgcca tcaacgcgca ggcggcctgc gacttcatcc cggtcggttt gtcgctggcg
    28441 gaagcccgtc aggacacggt tcgcgtcggt gtcccttctg tactggtgag ccacttttta
    28501 accggcgcac cgactgtact gatcgcctgg tttgtctccg gttttatcta tcaatagagg
    28561 ctgaaacatg accgttattt atcagaccac catcacccgt atcggcgcga gtgccactga
    28621 cgccctcagc gaccagatgc tcatcacctt tcgtgaaggc gcgccagcgg acctcgaaga
    28681 gtattgcttc attcattgcc acggcgagtt gaaaggtgca ctccatcccg gtttgcaatt
    28741 tacgctcggg cagcatcgct atccggtgac cgctgttggc agcgtggcgg aagacaacct
    28801 tcgcgaactg ggtcatgtca ccctgcgctt cgatggttta aacgaagcgg aatttccggg
    28861 cactgtccat gtggcaggcc ctgtacccga cgatatcgcg ccgggatcgg ttttgaagtt
    28921 tgaatctgtt aaggagtaaa aaatgaatca ggttgccgtt gtcatcggtg gtgggcaaac
    28981 cttaggcgcg ttcctgtgcc acggtctggc tgccgagggg tatcgcgtcg cggttgtcga
    29041 tattcagagc gacaaagccg caaatgtggc acaagaaatt aacgccgaat atggtgaagg
    29101 aacagcgtat agttttggtg ctgacgccac cagcgagcaa agcgtcctgg cgctctctcg
    29161 tggggtagat gaaatctttg gtcgcgtgga tttgctggtc tacagcgccg gaatagcaaa
    29221 agcagccttt atcagcgact tccagctcgg cgattttgac cgtgaatctg gtgggttatt
    29281 tcctgtgtgc ccgtgaattt tctcgtttga tgatccgcga cgggattcag ggtcgcatta
    29341 ttcagatcaa ctcgaaatcc ggcaaagtgg gcagcaaaca caactctggc tacagcgcag
    29401 cgaaatttgg tggcgtcggg ctgactcaat cactggcgct ggatctggcg gagtacggca
    29461 ttacggtgca ttcactgatg ctcggtaacc tgctgaaatc gcctatgttc cagtcactgt
    29521 taccccaata cgcgaccaag ctgggtatca aaccggatca agtcgagcag tattacatcg
    29581 acaaagtacc gctcaaacgc ggctgcgatt atcaggatgt gctgaatatg ctgctgctct
    29641 atgccagtcc taaggcgtcg tactgcaccg gacagtcgat caatgtcacc ggcggtcagg
    29701 tgatgttcta atcaacagcg gagatccgtt aaggatctcc gtgagactaa tagaatgcct
    29761 gatgcgctac gctcatcagg catacaggac ttccgccact acattaagga aaagttatgg
    29821 tatccgcact catcaccgtc gccgttatcg cctggtgtgc gcaactggcc ttaggcggct
    29881 ggcaaatttc tcgttttaac cgtgccttcg acacactatg ccagcaaggg cgggttggcg
    29941 tgggccgttc cagcgggcgc tttaaaccac gggtcgtggt cgccatcgcc ctggacgatc
    30001 agcagcgcat cgtcgacacc ttgtttatga aaggactgac cgtcttcgca cgaccgcaaa
    30061 aaattcccgc aattaccggt atgcatgcgg gtgatttaca gcccgatgtg atctttcccc
    30121 atgatccact atcacagaat gctctatcat tggcgcttaa actgaaacgt ggataatttc
    30181 gttgtgaatg ttacttgctt gcgaagttat cattttgaaa cctaaatcag gtaatcacgc
    30241 ccatgaaacc tcgtcagcgt caggccgcca ttctggagta tctgcaaaag cagggtaaat
    30301 gctcggttga agaattggcg caatactttg acaccacagg cacaaccatt cgcaaagatc
    30361 tggtcattct ggaacatgca ggaaccgtca ttcgtactta tggcggagtg gtgttgaata
    30421 aagaggaatc cgatccgcct atcgatcata aaacactcat caacacccac aagaaagagc
    30481 tgatagcaga agctgccgtt agttttattc atgatggcga ttcgatcatt cttgatgctg
    30541 gcagtaccgt tttgcagatg gttcccctgc tctcgcgctt taataacatc acggtgatga
    30601 ccaacagtct gcatatcgtc aatgcgctat ctgaactgga taacgaacaa actatcctga
    30661 tgccaggcgg aacgtttcgt aaaaaatcgg cctcatttca cggacagctg gcagagaatg
    30721 ccttcgaaca tttcagtttc gataaattgt ttatgggcac cgacggcatc gatctcaatg
    30781 cgggcgtaac cacctttaac gaggtttata ccgtcagtaa ggcaatgtgc aatgccgcgc
    30841 gcgaagtgat tttgatggcg gactcatcaa agtttggccg taaaagcccc aacgtagttt
    30901 gcagtcttga aagcgtcgat aagctgatta ccgacgcagg tatcgatccg gcgtttcgtc
    30961 aggcgctgga agagaaaggg atcgatgtga tcataaccgg agagagcaat gagtgaagca
    31021 ctactgaacg cgggacgtca gacgttaatg ctggagttgc aggaagcaag ccgtttaccg
    31081 gaacgtctgg gcgatgattt tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa
    31141 gtggtggttt cgggaattgg caaatcgggc cacattggta agaaaatcgc cgcaacactt
    31201 gccagtaccg gcacatcggc tttttttgtc catccggcag aagcactgca cggcgatctg
    31261 gggatgattg aaagccgcga tgtgatgctg tttatctctt actccggtgg cgcgaaggaa
    31321 ctggatctga ttattccgcg tctggaagat aaatctatcg cgctgctggc gatgaccggc
    31381 aaaccgacgt caccgctggg cctggcggca aaagcggtgc tggatatctc cgtagaacgc
    31441 gaagcctgcc cgatgcacct tgcgccgacc tccagcaccg tcaataccct gatgatgggt
    31501 gacgcgctgg cgatggcggt catgcaggcg cgcggattta atgaagaaga ttttgcccgc
    31561 tcccacccag ccggggcact gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc
    31621 cgtgacgatg ccatcccaca ggtggcgtta accgccagcg tgatggatgc gatgctggaa
    31681 ctcagccgca ccggtctggg gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc
    31741 gtctttaccg acggcgattt acgtcgctgg ctggtaggcg gcggcgcact caccacgcca
    31801 gttaatgaag cgatgacgac aggcggcacc acgttacagg cgcaaagtcg cgccatcgac
    31861 gccaaagaga tcctgatgaa gcgcaaaatc actgccgcac cggtggtgga tgaaaacggc
    31921 aaactcaccg gcgcaattaa cctgcaggat ttctatcagg ccgggattat ttaatccttc
    31981 attcccagac gtttcgccag ccgatgcagg ttggcgacgt cggtttccag catccgcgcg
    32041 caggcagccc agttatgatg attttgtgcc agtgcctggc gaatagtttc acgctggaac
    32101 gcttctgtcg cttcacgcag gttttgctta acaacgggca ccgccgccgc ttctggcggc
    32161 ggcaacgtca cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc atcgccgttg
    32221 cgggtggctc tcgccagcac taccgcccga tgaatagcat gttccagttc gcgcacgttc
    32281 cccggaaaac gatagtgttg cagtaaattt cgcgctccgg cacttaatac cacgcgggag
    32341 agccccagcc gcaaacgaca ctgctcgcag aaataccccg ccagcagaat gacatcatcg
    32401 ccccgctcac gcagcggcgg caccgaaagt ggaaacacgc tcaggcgatg aaacaagtca
    32461 gcgcgaaatc gccctgccat cacctcttcg cgtaagtcgc ggttagtcgc cgccagcacg
    32521 cgcacatcga cccgcaaact acggtcatcg ccaacgcgct gaatatcgcc atactgcaac
    32581 accctcagca gcttggcctg caatgccaac gacaactcgc cgatctcatc cagaaacaga
    32641 gtgccgttat ccgccatttc aaacttcccg ctgcggttac tgatagcgcc agtaaacgct
    32701 cctttcacat gcccgaacaa ctcactttcc gccacacttt ccggcagtgc agcacagttg
    32761 agatagacca gcggattcac cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc
    32821 tccttaccgg ttcccgtctc accgctgatc aggacgttga gatcggacgc cgccacaatc
    32881 tcaatctctt ttttcagttg cgtcatgcca ggggacaagc caatcatctg cgtctgtttc
    32941 accgcttcaa acggcgtggc atcgcctggc atcatattct ggctttccag ttgttcaatc
    33001 agcaacgcat tgcttaacgc tcccgccgcc agcgcagcaa tcagccgtag ctcttcgtcg
    33061 ctgaaaacat cgaactgatc gggctgcatc ccgtcgagcg tcagtgcgcc gatcaggttt
    33121 tgcccggcaa acaatggcag accaacgcag gcgtgaacct tcagactctc ctgcccagga
    33181 atcaaaccgt catagggatc gggcaattcg ctgtctgcgg gaaagcgcac cacatccccg
    33241 gcgcgggcaa tcgcttccag ccgtggatgc ccttccagcg caaagcgtct accgagtaca
    33301 tcctttgcca gaccgtcgat ggcaagcgga ataaactgcc gcgaatcgta acgtagcaac
    33361 gcagacgcat cgcactccag cacctgacgt agcgtggtga tcaggcgctg aaaacgatcc
    33421 tgatgaccaa tcccacgctg caattcgatg gcgatattcg ccagcacatc aacggaaaaa
    33481 ctcatctttg cctcactgtc aatttgacta tagatattgt catatcgacc atttgattga
    33541 tagtcatttt gactactcat ta
//
LOCUS       DAZPEP010000030        32841 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZPEP010000030 DAZPEP010000000
VERSION     DAZPEP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32841
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.030"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            267..1925
                     /locus_tag="ACHUT9_001411"
     CDS             267..1925
                     /locus_tag="ACHUT9_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFR1347919.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="ACHUT9_001412"
     CDS             complement(1922..2878)
                     /locus_tag="ACHUT9_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFR1347920.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="ACHUT9_001413"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="ACHUT9_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFR1347921.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="ACHUT9_001414"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="ACHUT9_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFR1347922.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUT9_001415"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="ACHUT9_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFR1347923.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUT9_001416"
     CDS             6534..6608
                     /gene="yljB"
                     /locus_tag="ACHUT9_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFR1347924.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUT9_001417"
     CDS             6707..7027
                     /gene="clpS"
                     /locus_tag="ACHUT9_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFR1347925.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUT9_001418"
     CDS             7058..9334
                     /gene="clpA"
                     /locus_tag="ACHUT9_001418"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFR1347926.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9740..9827)
                     /locus_tag="ACHUT9_001419"
     tRNA            complement(9740..9827)
                     /locus_tag="ACHUT9_001419"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9791..9793),aa:Ser,seq:gga)
     gene            complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUT9_001420"
     CDS             complement(10081..10299)
                     /gene="infA"
                     /locus_tag="ACHUT9_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFR1347927.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUT9_001421"
     CDS             complement(10584..11288)
                     /gene="aat"
                     /locus_tag="ACHUT9_001421"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFR1347928.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUT9_001422"
     CDS             complement(11330..13051)
                     /gene="cydC"
                     /locus_tag="ACHUT9_001422"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFR1347929.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUT9_001423"
     CDS             complement(13052..14818)
                     /gene="cydD"
                     /locus_tag="ACHUT9_001423"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFR1347930.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUT9_001424"
     CDS             complement(14941..15906)
                     /gene="trxB"
                     /locus_tag="ACHUT9_001424"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFR1347931.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16451..16945
                     /gene="lrp"
                     /locus_tag="ACHUT9_001425"
     CDS             16451..16945
                     /gene="lrp"
                     /locus_tag="ACHUT9_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFR1347932.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHUT9_001426"
     CDS             17080..21147
                     /gene="ftsK"
                     /locus_tag="ACHUT9_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFR1347933.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            21302..21913
                     /gene="lolA"
                     /locus_tag="ACHUT9_001427"
     CDS             21302..21913
                     /gene="lolA"
                     /locus_tag="ACHUT9_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFR1347934.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21924..23267
                     /gene="rarA"
                     /locus_tag="ACHUT9_001428"
                     /gene_synonym="mgsA"
     CDS             21924..23267
                     /gene="rarA"
                     /locus_tag="ACHUT9_001428"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFR1347935.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23358..24650
                     /gene="serS"
                     /locus_tag="ACHUT9_001429"
     CDS             23358..24650
                     /gene="serS"
                     /locus_tag="ACHUT9_001429"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFR1347936.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHUT9_001430"
     CDS             24889..27333
                     /gene="dmsA"
                     /locus_tag="ACHUT9_001430"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFR1347937.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHUT9_001431"
     CDS             27344..27961
                     /gene="dmsB"
                     /locus_tag="ACHUT9_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFR1347938.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHUT9_001432"
     CDS             27963..28826
                     /gene="dmsC"
                     /locus_tag="ACHUT9_001432"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFR1347939.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHUT9_001433"
     CDS             complement(28862..29488)
                     /gene="ycaC"
                     /locus_tag="ACHUT9_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFR1347940.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29803..30951
                     /locus_tag="ACHUT9_001434"
     CDS             29803..30951
                     /locus_tag="ACHUT9_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1347941.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31161..32591
                     /locus_tag="ACHUT9_001435"
     CDS             31161..32591
                     /locus_tag="ACHUT9_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1347942.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(32592..>32841)
                     /locus_tag="ACHUT9_001436"
     CDS             complement(32592..>32841)
                     /locus_tag="ACHUT9_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1347943.1"
                     /translation="DDVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITER
                     PGFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         8058 a         8033 c         8814 g         7936 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatctatc atgtggagta gattggtcag gcaaataagc tcttgtcagc
     6661 ggcagggcgt tctgccgata accgtaaccg aagatgataa ctgacaatgg gtaaaacgaa
     6721 cgactggctg gactttgatc aactggcgga agaaaaagtt cgcgacgcgc taaaaccgcc
     6781 atctatgtat aaagtgatat tagtcaatga tgattacact ccgatggagt ttgttattga
     6841 cgtgttacaa aaattctttt cttatgatgt agaacgtgca acgcaattga tgctcgctgt
     6901 tcactaccag gggaaggcca tttgcggagt ctttaccgcc gaggttgcag aaaccaaagt
     6961 ggcgatggtg aacaagtacg cgagggagaa tgagcatcca ttgctgtgta cgctagaaaa
     7021 agcctgaatg caggcataaa aattggggga ggtgcctatg ctcaatcaag aactggaact
     7081 cagtttaaat atggctttcg ccagagcgcg cgagcaccgt catgagttta tgaccgtcga
     7141 gcacttgtta ctggcgctgc tcagtaaccc atctgcccgg gaggcgctgg aagcgtgttc
     7201 tgtggatttg gttgcgctcc gtcaggaact ggaagccttt attgaacaaa ccacacccgt
     7261 tctgcctgcc agtgaagagg agcgcgacac acagccgacg ctgagttttc agcgtgtact
     7321 gcaacgtgcg gtcttccatg tccagtcctc cggtcgcaat gaggttaccg gtgcaaacgt
     7381 tctggtcgct atctttagcg aacaggagtc gcaggcggca tatctgttgc gtaaacatga
     7441 agtcagccgt ctcgatgtgg tgaattttat ctctcatggc acgcgtaaag acgagccgac
     7501 acagtcttct gatcctggca gccagccaaa cagcgaagaa caagctggtg gggaggaacg
     7561 tatggagaat ttcacgacga acctgaatca gcttgcgcgc gtgggcggaa tcgacccact
     7621 gattggtcgt gagaaggagc tggagcgtgc tattcaggtt ctctgccgtc gccgtaaaaa
     7681 caacccgctg ctggtggggg aatctggtgt cggtaaaacc gcgattgcgg aaggtcttgc
     7741 ctggcgaatt gttcagggcg atgtgccgga agtgatggct gactgtacga tttactctct
     7801 cgatatcggt tctctgttag cgggcacaaa atatcgcggc gactttgaaa aacgttttaa
     7861 agcgttgctc aagcagctgg agcaggacac taacagcatc ctgtttattg atgagatcca
     7921 caccattatc ggtgcggggg cagcgtctgg tggtcaggtt gatgcggcta acctaatcaa
     7981 accgttgctc tccagcggta aaattcgtgt aattggttcg acaacctatc aggagttcag
     8041 caacattttc gagaaagacc gtgctctggc gcgtcgcttc cagaaaattg atattactga
     8101 accgtcaatc gaagaaactg ttcaaatcat caatggcctg aaaccgaagt atgaagcgca
     8161 ccacgacgtg cgttataccg caaaagcggt gcgtgcggcg gtagagctgg cggtgaaata
     8221 catcaatgat cgtcatctgc cggataaagc gattgatgtt atcgacgaag cgggcgctcg
     8281 cgcacgcctg atgccggtaa gcaaacgcaa gaaaaccgtt aatgtggcgg atattgagtc
     8341 cgtggtggcc cgtattgcac gcattccaga gaagagtgtt tctcagagtg accgcgatac
     8401 cctgaaaaac ctcggcgatc gcctgaaaat gctggtcttc ggtcaggata aagccattga
     8461 ggcgctgact gaagccatta agatggcgcg tgcaggttta ggtcacgaac ataaaccggt
     8521 cggttcgttc ctgtttgccg gtcctaccgg ggtcgggaaa acagaggtga cggtacagct
     8581 ttcgaaagcg ttgggcattg agcttctgcg ctttgatatg tccgagtata tggaacgcca
     8641 taccgtcagc cgtctgattg gtgcgcctcc gggatacgtt ggttttgatc agggcggttt
     8701 gctgaccgat gcggtaatca agcatccaca tgcggtgctg ctgctggacg aaatcgagaa
     8761 agcgcatccg gacgtgttca atattctgtt gcaggtgatg gacaacggta cgctgaccga
     8821 taacaacgga cgcaaagcgg acttccgtaa cgtggtgctg gtgatgacca ccaacgccgg
     8881 ggtacgggaa actgagcgca aatccattgg tcttatccac caggataaca gcaccgatgc
     8941 gatggaagag atcaagaaga tctttacacc ggaattccgt aaccgtctcg acaacattat
     9001 ctggtttgat catctgtcaa ccgacgtgat ccatcaggtg gtggataaat tcatcgtcga
     9061 gttgcaggtt cagctggatc agaaaggtgt ttctctggaa gtgagccagg aagcgcgtaa
     9121 ctggctggcc gagaaaggtt acgaccgggc aatgggcgct cgtccgatgg cgcgtgtcat
     9181 ccaggacaac ctgaaaaaac cgctcgccaa cgaactgctg tttggttcgc tggtggacgg
     9241 cggtcaggtc accgtcgcgc tggataaaga gaaaaatgag ctgacttacg gattccagag
     9301 tgcacaaaag cacaaggcgg aagcagcgca ttaatctaat tgttaggtag gttggtgaag
     9361 tccgtaatct cgaaagaggt tacggacttt ttgtttatgg ggtggaacgg tgaaaaccct
     9421 atttttggag gtgaaggtaa tttgttgata attagtgctg ctggaaaagg taaggataaa
     9481 aaagggtgac agcaggaaaa tgagatggtt ttgctttatt aacaacggac taaacgtgta
     9541 gtatttgagt tcactgccgt acaggcagct tagaaatcga cggggtgcgg taaaaccttt
     9601 gcgaacgcgt tcactgccgt acaggcagct tagaaattca caggtaacat actccacccg
     9661 cccaccatgt tcactgccgt acagaaagat aaaatgcgaa aaaaaagctc gcactttcgt
     9721 acgagctctt ctttaaatat ggcggtgagg gggggattcg aacccccgat acgttgccgt
     9781 atacacactt tccaggcgtg ctccttcagc cactcggaca cctcaccaaa ttgttttgct
     9841 accaaacctc atgggtggca acggggcgct actataggga gttggagtaa aacggtcaag
     9901 aagaatttta atgataatta ttgtttgctc atactgtaaa caagttgtgc agtatatcta
     9961 catcgagaca agttacggac ttatacttcc aaagtacttc atacatatca caaaataaaa
    10021 aggccggtta aaccgacctt ttactcgttc tttctcttcg cccatcaggc ggtaaaacaa
    10081 tcagcgacta cggaagacaa tgcggccttt gctcaggtcg tacggggtca gttcaacagt
    10141 cactttgtcg cccgtcagga tgcggatgta gtttttgcgc attttaccgg agatgtgtgc
    10201 agtaaccacg tgaccgtttt ctaactctac gcggaacatg gtattaggca acgtttcaag
    10261 aacggtacct tgcatttcaa tattgtcttc tttggccatc taatcctctg gggtatcact
    10321 accgtaattt gaaccggcaa gataatgccg aagttctgta aataagtaaa gatttgcgcg
    10381 ctaaatcgca acaaacaggt tcggcacatt actccgaaaa cacacggcta agccgcacca
    10441 aaagcgcaac gtataaggga gcggtgagat aaacgatggg cgttacctga cgcgaaaaat
    10501 tccttatcgg cagcggggta atgagcataa ccaactctgc gaccgcaatt ataacactct
    10561 ggggagaaat gtgccgaaaa cattcatttt tgtggtgaaa acaagcatcg tggcacccag
    10621 aaattattcg gcaatcgtcc gaggcgcatt tgattgagat aattcagata atcccgacgg
    10681 ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa ggacctggca gtcgataagc
    10741 ttaccgccat gactgataaa ttcctcacag aataccagaa gcgccgtttt agacgcattt
    10801 tccatccggc tgaacatgga ctcgccacaa aatagcgttc cctgggccac gccgtacata
    10861 ccgccgacaa gctcatcttc atgccagact tcaatggagt gggcatgacc gagttcgtga
    10921 aggcgatggt aggcttcgac cacgccacgc gtgatccagg ttccttcttc gcgatcgctg
    10981 gcacaacctt caatgacctg accaaaagcg taattcatcg tgacacgata gggcgagcgt
    11041 ttatgaaatc gcttcatact acggctgata tgcagtgatt caggccaaag caccgcgcga
    11101 ggatcgggcg accaccagag gatgggggcg cctggagaaa accacggaaa aataccacgc
    11161 tggtaagcca ttaacaggcg cgcagggcta agatcgcccc ccagagccag caggccgtta
    11221 ggctcacgta atgcgccttc cggggaaggg aaggctattg aatggcgaga aagctgaacc
    11281 aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc aataatagct tacaaaccct
    11341 gcttaaactg gtaataacgc ccctgtctgg caagcagttc tgcgtgagta ccttgctcaa
    11401 taatttgccc gttgtccatc actattattt gttggaaacg agagagtccg cgaagtcgat
    11461 gggtgaccat taacaccgtt ttctcacgca tcatttctgc aagcaattca aggatctggc
    11521 tttcggttgt ggcatctaag ccttcggtag gttcatccag caacaccagt ggcgcatcat
    11581 gtaacagcgc acgggcgata gccagacggc gcagttcacc accggagagc tggcgtccgc
    11641 cttcacctaa ccaactgttg agacctgcgt cctcaagcag cttctccaga ccaacgcgac
    11701 gcaagatctc cgacagagcc tcatcactac tgccaggcga ggcgagtaac agattatcac
    11761 gcagcgtggc gctaaacaga tgcactcgct gaggaacaac gctgatggtc tgtcgtagag
    11821 ccgcttcatt caggctggct atggggctat cgttaagcaa aatctcgccc tgttgcgggt
    11881 cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc gcatccggtt cgcccgagaa
    11941 tcgcaatatg ttccccggcg tttacctgaa gagaaatccc tttaagtgcc tgttgggatt
    12001 gctccggata agtgaactga acatcccgta atgtcagcga aacgcgatcg gcaacacgag
    12061 tttgggtatc agggaaggtg acttccggtt tttgatccgt taaatcagtg atccgaacgg
    12121 cagaggcaat gacttgcccc agatgctgaa atgcacccgt tactggtgcc agtgcttcaa
    12181 acgcggctaa cgcgcagaag acaaacaggg caattaacgc gccgggttga gcattgccgc
    12241 caacgccgcc agacgccatc cacagcatca ggatcaccgc taacgcgcca atgagcagca
    12301 ttatcgcttg cgacaatgcg gtcagttcag attgacggcg ttgcgcttcc agccattgaa
    12361 tttctgtatt ctctagttgc gtgcgataac gatcgctggc accaaaaatg gtcagctcag
    12421 cttgcccttg cagccaggcc gtcagttgtt ggcgatactg tccgcgaaga tgagtcagat
    12481 tttgcccggt gctttttccc gcacgataaa acagcggtgg catcaggaaa agcgtcagta
    12541 acataatgcc gcccagcgta aaggcgaggg tgaaatcaag gaaacttaac ccgactgtca
    12601 ccaccataat caccacaaaa gcgcccacca gcggcgagat aacgcgcagg taaagatgat
    12661 cgagcgtatc aacatccgcc accacgcgat tgagcaattc gccctgacga tagcgcgcca
    12721 gtccggcagg ggagaggggc aacaatttgc tgaaggtgta aatgcgcaga tgctgcaaca
    12781 cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata acgcccggca gtacgggtga
    12841 ttgcggcacc acgcacgccc gcagcgggta gcatatagtt gaagctgtac agtccggcaa
    12901 cccccgcaac cgctgaggcc gagaggaacc agccggaaag tgtcaacaga ccgatactgg
    12961 cgagcagcgt cacaattgcc agcacaatac caagacttaa catccattta tgacgtttat
    13021 acagtgccag atagggtagc aaagcgcgca tttaaatctc ctcctgacga tgggccagta
    13081 atgtggcgaa tgggccacca gccacactta attccgcgta acgtccttgc tcaataatct
    13141 gaccatcctg cattacccaa atgacatccc agtcagcaag atcttctaac tggtgggtga
    13201 ccattaacgt tgtctggcgc agagaggcgg cattcagcgc ctccattacg cgctgttcac
    13261 tgtgagcatc aaggctggca gcgggttcat ccaacagtaa tagcgaacag ggatttagta
    13321 acgcacgggc caccgccacg cgctgcgcct gccccacgga aaggcgggca gcctggtcgc
    13381 caacaggcgt atcaacgcct tgtggcagga gcggtagaaa ctcgctgacc caggcgttat
    13441 ccagcgctgc ttgtaattca tgttcgctgg catcaggtcg cgccagtagt acgttatccc
    13501 gtaatgttgc tgccggtaat tgtggatttt gcccaaccca ggagagatgt ttacgccatg
    13561 attctggtga taaatcgcgt aattctatcc cgttgattcg tagcgatccc tgatatgaga
    13621 gaaaaccaga aagcgcgttc aacagtgaac ttttacctga accgctgcga ccaaccaaca
    13681 cggcacgttg gcctgctggc aaagtaaagt tcagcggtcc ggccagcgtt ttaccttccg
    13741 gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg atcggtcgat gctaattcca
    13801 cctcgccacg ctgcggatgg gcgagcgggg tttccataaa cgttttcaga ctgtcagctg
    13861 caccaacagc ttgggcttta gcatgataaa acgtaccgag atcgcgtaat ggctggaaaa
    13921 actctggcgc aaggatcagg gccagaaaac ccgcagccag cgtcacgcca gtatcgtagt
    13981 gaccaaaatc cagctcgccg agataggaaa aaccaaagta gaccgccacc agagcaattg
    14041 acagcgaggt aaaaaattcg agaatgccgg aggataaaaa tgccagccgt agcacttcca
    14101 ttgtccgttg gcggaaatct tccgaagcag aacgaatact ttcaatttca gcttcaccac
    14161 gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc gaggaaatgc ccacttaagc
    14221 gagcaagagc gagaaagtta cgtcggttag catcggcagc ccccattcca accagcgcca
    14281 taaacaacgg aattaacggt gcagtgccca gcagaatgag cgccgcagcc cagtttgagg
    14341 ggaagattgc caccacaatc agcaacggca ccgacactgc cagcgccatt tgcggcaggt
    14401 agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag taccagcgtc gcccagctcc
    14461 ccgcaggttt accctgaatc cacgctggcc ctgcttgttg cagacggtcg agaacctgac
    14521 ggcggatggc aaagcggata tgctgcccgg cgtgataacc cacccgttcg cgtaaccaga
    14581 ccgcccatgc gcgcagtaca aaggtcagaa ccagtaacgt aaagggaagc agcagggctt
    14641 cacggggaat attctccata atcatatgtt gcagaattcg cgccatgaac caggcctggg
    14701 caatgatcaa tatgccgctt acaaagccca gcagacgaga aatattcagc caacgttggg
    14761 agatgacgct ttgctgtttt aaccagcggg ttaactcttt ttgacgagat ttattcattg
    14821 cacgcttagc aggtgagtta tcagaattat ttgcagagca atgttacaac ggggaaaaaa
    14881 taaaggcgac ccatagtcgc aaggtgtcgc cttctttact tttgttactg atttgtaaaa
    14941 ttattttgcg tcagctaaac catcgaggta gcgttccgca tcaagtgctg ccatgcagcc
    15001 agtaccggcc gaagtaatgg cctggcgata aatgtgatcc atcacgtcgc ctgcggcaaa
    15061 gacgccagga atgctggtct gggtggcatt accatgaata cccgactgta ctttgatgta
    15121 gccgttttcc agttccagct gcccttcgaa aatcgcagta ttcgggctgt gaccgatagc
    15181 aacaaacaga ccggcaacgt cgagtgactc gatgttatcg ctgttttgcg tatcgcgcag
    15241 acgaacgcca gtgacaccca tttgatcgcc ggtcacttct tccagcgtac ggttggtgtg
    15301 cagaatgatg ttgccgttct ccactttatc catcagacgc ttaataagga ttttttccgc
    15361 gcggaaaccg tcacggcggt gaatcagatg cacttccgaa gcgatgttag acagatacag
    15421 cgcctcttca accgcggtat tgccgccgcc gatgaccgca actttctggt tgcgatagaa
    15481 gaaaccgtcg caggttgcac aagcagaaac cccacggcct ttaaaggctt cttcagaggg
    15541 caggccgaga tagcgtgcag aagctccggt ggcaataatc agcgcgtcgc aagtgtattc
    15601 gccgttatcg ccattcagac ggaacggacg gttttgcaga tccaccttgt tgatatgatc
    15661 aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc atgcgctcca ttaataacgg
    15721 accggtcaga tcgtttggat caccaggcca gttttccact tccgtggtgg tggtcagttg
    15781 gccgcctttt tccatgccgg taatcagcac aggttgcagg ttggcgcgcg ccgcgtagac
    15841 agcagcggtg tatcccgccg ggcctgaacc caggataagc agtttactgt gtttggtcgt
    15901 gcccatgaga tccccatagt tgttggcaga caatgggcag gattgtaggg aatttacaga
    15961 cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc aatcggcagc atcgataagc
    16021 aggtcaaatt ctcccgtcat tatcacctct gctacttaaa tttcccgctt tataagccga
    16081 ttaaatgatg aataaacgcc cctgttaatg aatatctggc atgttgtact aaaaatcgat
    16141 gttttgcttt gacaatcccc tggtgttttg cgaaaacatt cgaggaagaa aaaaaacagt
    16201 attcttatat gcgcataacc atgcatgtaa ataccatgtt taccgtgcta gtgaaatcta
    16261 cgtatggcgt ggacagacgc cattcgtgat gtcgatagct gccacaaggc aacggtcttc
    16321 tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg atttcggtct atcgtgacgg
    16381 gtagcgactc tgaacagtga tgtttcaggg gcagacagga gtagggaagg aatacagaga
    16441 gacaataata atggtagata gcaagaagcg ccttggcaaa gatctcgacc gtatcgatcg
    16501 taacattctt aatgagttgc aaaaggatgg gcgtatttct aacgtcgagc tttctaaacg
    16561 tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt cggctggaaa gacaagggtt
    16621 tattcagggc tatacggcgc tgcttaaccc ccattatctg gatgcatcac ttctggtatt
    16681 cgttgagatt actctgaatc gtggcgcacc ggatgtgttt gaacaattca ataccgctgt
    16741 acaaaaactt gaagaaattc aggagtgtca tttagtatct ggtgatttcg actacctgtt
    16801 gaaaacacgc gtgccggata tgtcagctta ccgtaagttg ctgggggaaa ccctgctgcg
    16861 tctgcctggc gtcaatgaca cacggacata cgttgttatg gaagaagtca agcagagtaa
    16921 tcgtctggtt attaagacgc gctaacacgg aacaggtgca aaatcggcgt attttgatta
    16981 cactcctgtt aatccataca gcaacagtac tggggtaacc tggtactgtt gtccgtttta
    17041 gcatcgggca ggaaaagcct gtaacctgga gagcctttct tgagccagga atacactgaa
    17101 gacaaagaag tcacattgac aaagttaagt agcggccgcc gccttctgga ggcgttgctg
    17161 atccttattg tcctgtttgc cgtctggttg atggctgcct tactaagctt taacccttcg
    17221 gaccccagct ggtcgcaaac ggcctggcat gaacctatcc ataatttagg tgggatgccc
    17281 ggtgcgtggt tggcagatac gctgttcttt atttttggcg tgatggctta caccattccc
    17341 gtcattattg tcggcggttg ttggtttgcc tggcgtcatc agtccagcga cgaatacatt
    17401 gattattttg ccgtttcgct acgcatcatt ggcgttttgg cgctcatcct tacctcctgt
    17461 ggtctggcgg caatcaacgc tgacgatatc tggtattttg cctccggtgg cgtcattggc
    17521 agcttactaa gcactacgct acaaccactg ctacacagta gcgggggaac tattgcgctg
    17581 ctctgcgttt gggcagcggg cctgacgttg ttcaccggtt ggtcatgggt gaccattgct
    17641 gaaaaactcg gcggctggat tttaaacatt ctcaccttcg ccagtaatcg tacccgtcgc
    17701 gatgatacct gggtcgatga agatgagtat gaagacgacg aagagtatga agatgaaaat
    17761 cacggcaaac agcatgaatc acgccgtgcc cgtattcttc gcggcgcgct agcgcgtcgt
    17821 aaacggttgg cggaaaaatt cattaatccg atggggcggc aaacagacgc tgcgttgttc
    17881 tccggtaagc ggatggatga tgacgaagag attacctaca ctgcacgcgg tgtggctgcc
    17941 gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc agccagaata tgacgaatac
    18001 gatccattat taaacggtgc gccaattacc gaacctgtcg ctgtagcagc tgctgctacc
    18061 acggcggcac aaagctgggc tgcgccggtt gaacctgtga ctcagacgcc gcctgttgcc
    18121 tctgttgatg ttccgccttc gcaacctaca gtagcctggc agcctgtacc gggtccacaa
    18181 acgggagagc cggttattgc tcctgcaccg gaaggttacc cacagcagcc acaatatgcg
    18241 cagcctgcag tgcaatataa tgagccgctg caacaaccag tacagccaca gcagccgtat
    18301 tatgcacctg cagctgaaca acctgtgcaa caaccttact acgctcctgc ggcggaacaa
    18361 cctgttcagc aaccgtatta cgcccctgcg ccagaacaac cggtggcagg taacgcctgg
    18421 caagccgaag agcagcaatc cacttttgct ccacagtcta cataccagac tgagcaaact
    18481 tatcagcaac cagccgctca ggagccgttg taccaacagc cgcaacccgt tgaacagcag
    18541 cctgttgtgg agcctgaacc cgttgtagaa gagacaaaac ccgcgcgtcc gccactttac
    18601 tattttgaag aagtggaaga gaagcgagcc cgtgaacgtg aacaacttgc ggcctggtat
    18661 caaccgattc cagaaccggt taaagaacca gaaccgatca aatcttcgct gaaagcacct
    18721 tctgttgcag cagtacctcc agtagaaacc gctgccgctg tttctccgct ggcatctggc
    18781 gtgaaaaaag cgacactggc gacgggggct gccgcaaccg ttgccgcgcc agtcttcagt
    18841 ctggcaaata gcggtggacc gcgtccgcag gtcaaagagg ggattggccc gcagttgcca
    18901 cgaccgaaac gtatccgcgt gccaactcgc cgtgaattgg cgtcttacgg tattaagctg
    18961 ccctcacagc gtgcggcgga agaaaaagcc cgtgaagccc agcgcaatca gtacgattct
    19021 ggcgatcagt acaacgatga tgaaatcgat gcgatgcagc aggatgaact ggcacgtcag
    19081 ttcgcccaga cacagcagca acgctatggc gaacagtatc aacatgatgt gcccgtaaac
    19141 gcagaagatg cagatgctgc ggcagaggct gaactggctc gtcagtttgc gcaaactcaa
    19201 caacaacgtt attccggcga acaaccggct ggggcgaatc cgttctcgct ggatgatttt
    19261 gaattttcgc caatgaaagc gttgctggat gatggtccac acgaaccgtt gtttacgcca
    19321 attgttgaac ctgtacagca gccgcaacaa ccggttgcac cgcagcagca gtatcagcag
    19381 ccacaacagc cggttgcgcc acagcagcaa tatcagcagc cgcaacagcc ggttgcgccg
    19441 cagccacaat atcagcagcc gcaacaaccg gttgcgccac agcagcaata tcagcagccg
    19501 caacaaccag ttgcgccgca gccgcagtat caacagccgc aacaaccggt tgcgccgcag
    19561 cagcagtatc agcagccaca acagccagtt gcgccacaac cgcaggatac cctgcttcat
    19621 ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac gccgctgcct
    19681 tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac ctttgcgctt
    19741 gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa agccgatgtt
    19801 gtcaattact ctccggggcc ggttatcact cgctttgaat tgaacctggc accgggcgta
    19861 aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc gacggtggcg
    19921 gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt accgaataaa
    19981 aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg cgataatccg
    20041 tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt tgccgatctg
    20101 gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc tgtcggtgtg
    20161 aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg tttcatcatg
    20221 atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct gttaacggaa
    20281 gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa cgagatggag
    20341 cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta taacgaaaaa
    20401 attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa gccgggtgac
    20461 agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt gttggttgac
    20521 gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat agcacgtctg
    20581 gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg tccatcggtt
    20641 gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt taccgtatcc
    20701 agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact gctggggatg
    20761 ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca tggtgctttt
    20821 gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg tcgcccacag
    20881 tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg tttcgatggc
    20941 gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga aaaacgcaaa
    21001 gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc agcgcgtatt
    21061 atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg taatcgtgaa
    21121 gtgctggctc caccgccgtt tgactaatta atgcattgcc ggataaggcg cggtagcgtc
    21181 gcatccggca ctctatcaac tgaaaattca gtattttctt ctttcctcaa gctgattatt
    21241 agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt gaggaataat
    21301 gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa gcagcgtttg
    21361 ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct tccacgccag
    21421 cttcacacaa aaagtgactg acggtagcgg cgcggcggtg caggaaggtc agggcgatct
    21481 gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg aaagcattct
    21541 ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag ctacggcaac
    21601 ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca accagtccag
    21661 cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga cgccgaaagc
    21721 cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca caatccatca
    21781 gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat cccagcaaaa
    21841 tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca cggtagatga
    21901 tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg gataatactt
    21961 ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc ggccagcaac
    22021 atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat ctgcattcta
    22081 tgatcctctg ggggccaccg ggtaccggca aaacaaccct cgctgaagtg attgcccgct
    22141 atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa gagattcgcg
    22201 aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt ctttttgttg
    22261 acgaagtgca ccgattcaac aaaagccagc aggatgcatt tctgccacat attgaagacg
    22321 gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt aattcggcac
    22381 tgctttcccg tgcccgtgtc tatctgttga aatccctggg tacagaggat attgagcaag
    22441 tactaactca ggcgatggaa gacaagaccc gtggttatgg tggtcaggat attgttctgc
    22501 cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc cgggcgttaa
    22561 atacgctgga aatgatggcg gatatggccg aagtcaatga tagcggtaag cgggtcctga
    22621 agcctgaatt attgaccgaa atcgccggtg agcgaagcgc ccgctttgat aacaaaggcg
    22681 atcgctttta cgatctcatt tccgcactgc ataagtcggt acgtggtagc gcacccgatg
    22741 cggcgctcta ctggtatgcg cgaattatta ccgctggtgg cgatccgtta tatgtcgcgc
    22801 gtcgttgcct ggcaattgct tccgaagatg tcggcaatgc cgacccacgg gcgatgcagg
    22861 tggcaattgc ggcctgggat tgctttactc gcgttggccc ggcggaaggt gaacgcgcca
    22921 ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc tacactgcgt
    22981 ttaaagctgc gctggcggat gctcgcgaac gcccggatta tgacgtgccg gttcatttgc
    23041 gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat cgttacgctc
    23101 atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgccggaa atagcacaaa
    23161 cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa aagctcgcct
    23221 ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt tatcgttgcg
    23281 gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc atttaattcg
    23341 ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca gacgcagtcg
    23401 ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc gctcttgaag
    23461 agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt aactcccgat
    23521 cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta cgtctggaag
    23581 tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct ttacaggctg
    23641 aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg ccggtaggta
    23701 aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag tttgactttg
    23761 aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt gcagctgcag
    23821 ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc atgcaccgcg
    23881 cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt gagaactatg
    23941 ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg aaatttgctg
    24001 gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac tatgcgctga
    24061 tcccaacggc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc gatgaagatg
    24121 atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc ggttcatatg
    24181 gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa atggtgcaga
    24241 tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat gcagaaaaag
    24301 tcctgcagtt gctgggcctg ccgtaccgta aaatcatcct ttgcactggc gacatgggct
    24361 ttggcgcttg caaaacttac gacctggaag tatggatccc ggcacagaac acctaccgtg
    24421 agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag gcacgttgcc
    24481 gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct ggtctggctg
    24541 ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt cgtattgaag
    24601 taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa tacccaattt
    24661 ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata ccgaacaata
    24721 attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat ctctttgatt
    24781 ttcaaattat tcgatgtata caagcctata tagcgaactg ctatagaaat aattacacaa
    24841 tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat gaaaacgaaa
    24901 atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa aacgacagcg
    24961 atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg gattgcgcac
    25021 gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag cgcctgtaca
    25081 gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga aatcaaatat
    25141 gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcaccaggt tcgcgcctgc
    25201 ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg accgcctgaa atatccgatg
    25261 aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga agaagcctac
    25321 gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga gtctatctat
    25381 ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc gccgggaaat
    25441 accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta cggcgactac
    25501 tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc agatggcaac
    25561 agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa ccctggcgaa
    25621 acgcgaatga gtggcggtgg ggtgacttac tatcttgaac aggcacgcca gaaatctaat
    25681 gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg cgaagatgag
    25741 tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc gtacgtcatg
    25801 atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg ctacgatgag
    25861 aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat tctgggtgaa
    25921 gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg tgttccggca
    25981 gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt tatcagccag
    26041 ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat ctcgatgctg
    26101 gcgattctga ccggtaacgt tggtattaat ggagggaaca gcggcgcgcg tgaaggttca
    26161 tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac cagcatttcg
    26221 atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct gcgtgatggt
    26281 gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta tgccggtaac
    26341 tgcctggtta accagcattc tgaaatcaac cgtacccatg aaatccttca ggatgataag
    26401 aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa atatgctgac
    26461 atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga tgcatcctgc
    26521 gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt tgaatgtaag
    26581 accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca acagtttact
    26641 gaaggccgta cccaggaaga gtggatgcgg catctgtatg cccagtcgcg ggaagcgatt
    26701 cctgaactgc caacgtttga agagttccgc aagcagggga tctttaaaaa gcgcgaccca
    26761 caagggcatc acgttgctta taaagccttc cgtgaagatc cgcaggcaaa cccactgact
    26821 acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc cgctacctgg
    26881 gaattgccag aaggcgatgt gatcgatcca ctgccgatct acacgccggg ctttgaaagt
    26941 tatcaggatc cgctgaacaa acagtatccg ctgcagctta cgggtttcca ctataaatct
    27001 cgcgttcact caacttacgg caacgttgat gtgctgaaag cagcttgccg tcaggaaatg
    27061 tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa agtcaggatc
    27121 tttaacgatc gtggtgaagt tcatattgag gcgaaagtga cgccacgaat gatgccaggt
    27181 gtggtcgcac tgggtgaagg tgcctggtat gacccggatg caaaacgtgt cgataagggt
    27241 ggttgtatta acgtactgac cactcaacgt ccgtctcctc tcgctaaggg gaatccgtca
    27301 catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa cccagtatgg
    27361 attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg cttgtaaaga
    27421 ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg ctggcggcga
    27481 ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc tgtcgatttc
    27541 gtgcaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg cgatgcataa
    27601 acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc gctactgcca
    27661 tatggcttgc ccgtatggcg caccgcaata taacgaaacg aaaggccata tgaccaaatg
    27721 cgatggttgt tatgaccgtg ttgcggaggg taaaaagccg atctgtgttg aatcttgtcc
    27781 gctgcgggcg ctggatttcg gccctatcga cgagctgcgt aaaaaacatg gcgatctggc
    27841 ggccgttgcg ccgttgccgc gagctcactt taccaaaccg aatattgtga tcaaacccaa
    27901 tgccaatagc cgcccgaccg gggataccac tggctatctg gcaaacccga aggaggtgtg
    27961 agatgggaag tggatggcat gaatggccgc tgatgatctt cacggtcttc gggcaatgtg
    28021 tggcgggtgg ttttatcgtt ctggctttgg cgctgctcaa aggcgacctg cgagcagaag
    28081 cccagcagcg tgttatcgcc tgcatgtttg gcttatgggt gctgatgggc attggcttta
    28141 tcgcttctat gctccatctt ggttcaccga tgcgcgcttt taactcgctc aaccgggtag
    28201 gggcttcagc actcagtaac gaaatcgcca gcggttcgat cttttttgcc gtaggcggca
    28261 tcggctggtt gctggcaatg ctgaaaaagc tgtcaccggc attgcgtacg ctttggctgg
    28321 tggtgacgat ggttcttggc gtcgtctttg tctggatgat ggtgcgtgtg tataacagca
    28381 ttgataccgt tccgacctgg tacagcatct ggacgccgat gggcttcttc ctgacgatgt
    28441 tcatgggcgg cccgttactg gggtacctgt tgctgagtct ggctggcgtc gatagctggg
    28501 cgatgcgtct gctgccagcc atttccgtac tggcactggt ggtgagtggc gtggtgtcag
    28561 tgatgcaggg cgcagagctg gcgactattc atagttctgt gcagcaggcc gcagcgctgg
    28621 tgccggacta tggtgcgctg atgtcctggc ggatcgtgct tttggccgtt gccctgtgct
    28681 tgtggattgc accacagcta aaaggttatc agcctgcggt tccgctacta tccgtctcat
    28741 tcattctgct tctggcaggg gaattaatcg gtcgcggcgt attctatggt ttgcatatga
    28801 ccgtggggat ggccgtcgca agctaatcag taacaaccgg ggtttcggcc ccggttctct
    28861 tttatttctg cttcgttaac gtgtcataac tggtcatcaa attacgataa tcaggaatat
    28921 ggttagagaa caacgtcgcc agtccttcaa tatcattacg ccagtcacgg tgcagctcgc
    28981 aagccacgcc gaaccatgtc atcaattgcg cacctgcttg cgacatgcga tcccatgccg
    29041 aatgccgggt aatttcatta aaggtccctg acgcgtcagt cacaacaaac acatcaaacc
    29101 cttcttcaat ggctgaaagc gccgggaatg ccacgcaaac ttcggttacc acaccggcaa
    29161 taattaactg ttttttacct gtcgctttga ctgcttttac aaaatcttcg ttatcccagg
    29221 cgttaatatt gcccgggcga gcaatgtaag gtgcatcggg gaattgtgct ttcagttctg
    29281 gaactaatgg gccgttagga ccggtttcaa aactggtggt aagaatagtt ggtaagttga
    29341 aatacttagc caggtcaccc agcgccagca cgttattttt aaacttatcg ggttcgatat
    29401 cccgtacaag ggaaagtaaa ccagcctggt gatcaacaag caaaacagca gcatcatttt
    29461 tatcaagacg aacatacggt ttggtcatcc tcttctcctt tcgagagtca gtggtacgcc
    29521 tgaaatcggt cagacgtgat gcattgctct gaaagcatag acgggaaata tgagtttgct
    29581 gtgaccatga aatttttcga ctgaacgaag cgtatgaaat ttgtgttaga tcaataaaaa
    29641 caatcagatg agattaatca tctcttcagt attagcgtta tgaatgagta atataaatcg
    29701 ataataccgc cgttgcgata tgcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    29761 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    29821 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgatcgc ggtgttaaat
    29881 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    29941 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaaa
    30001 cgcattggtt ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30061 ggccttggcc tgatgattgg attctggagt tggttggcgt ggcgttttgt cgcgggcgtc
    30121 ggctgtgcca tgatttgggt agtcgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30181 aaccgtgggc gtttgcttgc tgcatacatg atggtttatt acgtgggaac gtttttaggc
    30241 cagttactgg tcagcaaggt ctcaaccgag ctgatgagcg tattgccgtg ggttacaggt
    30301 ttgacattgg cagggatctt accgctgttg tttacgcgtg tcctgaatca gcaggctgaa
    30361 aaccatgatt cgacgtcaat tacggcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    30421 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gcctctgtac
    30481 ctcaatcaca aaggggtgag caatgccagt attggtttct ggatggcggt actggtcagt
    30541 gcgggtatcc tcggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    30601 gttttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta ttgcgatgct tagccaggcg
    30661 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    30721 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaagcctta
    30781 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    30841 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    30901 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    30961 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31021 ttattccaaa ttttattcgt ttaaaaataa aatgtgtagc aggttataat tttgcatttc
    31081 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctataa
    31141 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagctg cgatggtaca
    31201 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactacg
    31261 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcgctc tatttcacac
    31321 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    31381 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    31441 gggtggtgca tattccctat ctggcacaaa aaccccaggc aattctgatt gcgctcggtt
    31501 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    31561 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    31621 aaatcgtcgg ttctgtggca gggattgcta tgtttgttat gtcgctcctg tatgtggcga
    31681 tggcggtaac cgcgcctgca attactgaag tgcatattgc gaccacaaac attacctggg
    31741 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    31801 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    31861 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    31921 cgctggcgat ggggatgatg tttgattcgc gtaatatccc ggatgactta atgaccaacg
    31981 gtcagtatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32041 tgatttacgc cattgcgaat accctgggac aagtagcggc gctggtattc tcgattgatg
    32101 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32161 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggta ctggtggcga
    32221 ttctgattat gctgccgact ctcggcattg gtgatatgaa caatctctat aaatggctgt
    32281 tgaaccttaa ttcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    32341 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    32401 tggcaatgac cgtcgggatt tggtgttttg cctttaccgc ctttgcctgt ttgacgggga
    32461 tcttcccgaa aatggaagcc ttcactgcag agtggacctt ccagttggcg ctgaatgttg
    32521 caacgccgtt tgtgctggta ggattaggac tgatattccc gctgctggcg cgtaaagcga
    32581 atagtaaata attattgtgg tcggtgacgc tgactctggc agatctgacc cgtttttaca
    32641 taatcgagaa atgcgcgcaa gccgcaggac atatactggc ggtttgggta atagatctgg
    32701 aagcctggac gctctgtgat ccagtcttcc agtacacatt ctaaacgacc ggtatcaagg
    32761 tactccttga tctgttcata gagcagatac ccgataccag cacccatcaa aacggcatcc
    32821 agttcagcat caacatcatc c
//
LOCUS       DAZPEP010000031        32752 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZPEP010000031 DAZPEP010000000
VERSION     DAZPEP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32752
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.031"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            71..313
                     /locus_tag="ACHUT9_001437"
                     /pseudo
     CDS             71..313
                     /locus_tag="ACHUT9_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(355..1431)
                     /gene="glpQ"
                     /locus_tag="ACHUT9_001438"
     CDS             complement(355..1431)
                     /gene="glpQ"
                     /locus_tag="ACHUT9_001438"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFR1347944.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(1436..2794)
                     /gene="glpT"
                     /locus_tag="ACHUT9_001439"
     CDS             complement(1436..2794)
                     /gene="glpT"
                     /locus_tag="ACHUT9_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFR1347945.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            3067..4695
                     /gene="glpA"
                     /locus_tag="ACHUT9_001440"
     CDS             3067..4695
                     /gene="glpA"
                     /locus_tag="ACHUT9_001440"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFR1347946.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4685..5944
                     /gene="glpB"
                     /locus_tag="ACHUT9_001441"
     CDS             4685..5944
                     /gene="glpB"
                     /locus_tag="ACHUT9_001441"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFR1347947.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            5941..7131
                     /gene="glpC"
                     /locus_tag="ACHUT9_001442"
     CDS             5941..7131
                     /gene="glpC"
                     /locus_tag="ACHUT9_001442"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFR1347948.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            7324..8226
                     /locus_tag="ACHUT9_001443"
     CDS             7324..8226
                     /locus_tag="ACHUT9_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1347949.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8267..9070)
                     /gene="yfaU"
                     /locus_tag="ACHUT9_001444"
     CDS             complement(8267..9070)
                     /gene="yfaU"
                     /locus_tag="ACHUT9_001444"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFR1347950.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(9088..10377)
                     /locus_tag="ACHUT9_001445"
     CDS             complement(9088..10377)
                     /locus_tag="ACHUT9_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1347951.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(10434..11639)
                     /gene="rhmD"
                     /locus_tag="ACHUT9_001446"
     CDS             complement(10434..11639)
                     /gene="rhmD"
                     /locus_tag="ACHUT9_001446"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFR1347952.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAVMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            complement(11654..12436)
                     /locus_tag="ACHUT9_001447"
     CDS             complement(11654..12436)
                     /locus_tag="ACHUT9_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFR1347953.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(12656..13858)
                     /locus_tag="ACHUT9_001448"
     CDS             complement(12656..13858)
                     /locus_tag="ACHUT9_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFR1347954.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(13958..14500)
                     /locus_tag="ACHUT9_001449"
     CDS             complement(13958..14500)
                     /locus_tag="ACHUT9_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFR1347955.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            14779..15204
                     /gene="nudI"
                     /locus_tag="ACHUT9_001450"
     CDS             14779..15204
                     /gene="nudI"
                     /locus_tag="ACHUT9_001450"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFR1347956.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(15243..15845)
                     /gene="ais"
                     /locus_tag="ACHUT9_001451"
     CDS             complement(15243..15845)
                     /gene="ais"
                     /locus_tag="ACHUT9_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFR1347957.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            16153..17292
                     /gene="arnB"
                     /locus_tag="ACHUT9_001452"
     CDS             16153..17292
                     /gene="arnB"
                     /locus_tag="ACHUT9_001452"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFR1347958.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            17296..18264
                     /gene="arnC"
                     /locus_tag="ACHUT9_001453"
     CDS             17296..18264
                     /gene="arnC"
                     /locus_tag="ACHUT9_001453"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFR1347959.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            18264..20246
                     /gene="arnA"
                     /locus_tag="ACHUT9_001454"
     CDS             18264..20246
                     /gene="arnA"
                     /locus_tag="ACHUT9_001454"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFR1347960.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            20243..21133
                     /gene="arnD"
                     /locus_tag="ACHUT9_001455"
     CDS             20243..21133
                     /gene="arnD"
                     /locus_tag="ACHUT9_001455"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFR1347961.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRANQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            21133..22785
                     /gene="arnT"
                     /locus_tag="ACHUT9_001456"
     CDS             21133..22785
                     /gene="arnT"
                     /locus_tag="ACHUT9_001456"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFR1347962.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            22782..23117
                     /gene="arnE"
                     /locus_tag="ACHUT9_001457"
     CDS             22782..23117
                     /gene="arnE"
                     /locus_tag="ACHUT9_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFR1347963.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            23117..23503
                     /gene="arnF"
                     /locus_tag="ACHUT9_001458"
     CDS             23117..23503
                     /gene="arnF"
                     /locus_tag="ACHUT9_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFR1347964.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(23497..23763)
                     /gene="pmrD"
                     /locus_tag="ACHUT9_001459"
     CDS             complement(23497..23763)
                     /gene="pmrD"
                     /locus_tag="ACHUT9_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFR1347965.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(23873..25228)
                     /gene="menE"
                     /locus_tag="ACHUT9_001460"
     CDS             complement(23873..25228)
                     /gene="menE"
                     /locus_tag="ACHUT9_001460"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFR1347966.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     D"
     gene            complement(25225..26187)
                     /gene="menC"
                     /locus_tag="ACHUT9_001461"
     CDS             complement(25225..26187)
                     /gene="menC"
                     /locus_tag="ACHUT9_001461"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFR1347967.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(26187..27044)
                     /gene="menB"
                     /locus_tag="ACHUT9_001462"
     CDS             complement(26187..27044)
                     /gene="menB"
                     /locus_tag="ACHUT9_001462"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFR1347968.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(27059..27817)
                     /gene="menH"
                     /locus_tag="ACHUT9_001463"
     CDS             complement(27059..27817)
                     /gene="menH"
                     /locus_tag="ACHUT9_001463"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFR1347969.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(27814..29484)
                     /gene="menD"
                     /locus_tag="ACHUT9_001464"
     CDS             complement(27814..29484)
                     /gene="menD"
                     /locus_tag="ACHUT9_001464"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFR1347970.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(29573..30868)
                     /gene="menF"
                     /locus_tag="ACHUT9_001465"
     CDS             complement(29573..30868)
                     /gene="menF"
                     /locus_tag="ACHUT9_001465"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFR1347971.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(30947..31252)
                     /gene="elaB"
                     /locus_tag="ACHUT9_001466"
     CDS             complement(30947..31252)
                     /gene="elaB"
                     /locus_tag="ACHUT9_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFR1347972.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(31307..31768)
                     /locus_tag="ACHUT9_001467"
     CDS             complement(31307..31768)
                     /locus_tag="ACHUT9_001467"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1347973.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            31833..32750
                     /gene="rbn"
                     /locus_tag="ACHUT9_001468"
     CDS             31833..32750
                     /gene="rbn"
                     /locus_tag="ACHUT9_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFR1347974.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         7957 a         8860 c         8183 g         7752 t
ORIGIN      
        1 tgacaccgct tttgcccttc atttgcagcg catagattcc accagccacg accactaata
       61 gtgcgcccag cagataaaat gtgaatttaa tcaggtggat acagcgatga attttattcg
      121 acagggatta ggcatcgcct tattaccaga gttaacgctg aaaaccattg caggtgaatt
      181 gtgttccgtt cctcacgaac caaccttcta tcgacagatt tcgttgttgg ctaaagaaaa
      241 gccggtagaa ggcagtccac tgtttttact acaaatgtgc atggaacaat tagtggcgat
      301 tggaaaaatt tgatatgaga caggatggcg catgaacgcc atcctgcagt agcattactc
      361 tttattaagg aattttactg ccttatcagg gaaatcagta aacagcccat ttacacccgc
      421 tttgttatac agagcatcat ataactgatt cacatcagta gtgtattcag gcagtttatc
      481 cgaccgcacg gtataaggat gcactaccag tttattctgc tgagcatctt gcaccatgcc
      541 agtgagtttg atattacccg gctgcgatgt ttcctcaatc aacatatggt aatccggccc
      601 gataccgtcg gcatattccg cgacttgctt catggcacct ggcttaaaca tccagtcata
      661 gctgtaatta acccagcttc cgtccggctg tttctgctgc gtttcattcc agtcggtata
      721 ggcaatcagc tgtaccagat tgagatccat gcccatttta ggttccagct cattcttaat
      781 acgcttcagc tcatcagcat caaaacattg caaataaact ttatcgtcct taccggtgta
      841 accatatttc ttcagcactt ccagcgtttt tgccgcaata tccttccctt cctgatgatg
      901 gaaccacggc gctttgattt ctggatagat accgatattt ttcccggtag agtgatttaa
      961 cccctgaaca aattcaatct cttcttcaaa ggtgtgcccc cggaagtcgg acttacccat
     1021 tgggaaacgc cccggataag tctgtacttt tttaccgttt tcaatatcga aaccttcggt
     1081 aaatttcagc gacttaattt catccagcgt gaaatctatc gcgtagtaac gaccgtcttt
     1141 gcgcgcccga tccgggaaac gatcggcaac atcagtaaca cgatcgagat aatggtcatg
     1201 cagaacaacc agatggtcgt ctttggtcat caccaaatcc tgttccagat aatccgctcc
     1261 ctgcgcatac gccatcgctt ttgctggcag cgtatgctcc ggcaaatatc cactggcacc
     1321 gcgatgggcg atgactattt tttcgttgct gtccgccgcc attgcactgc ttcccatgac
     1381 tatagtgctc atcatgatcg ccatgctaag gtttttcagc ttcaatttca tgccattagc
     1441 ctccgttgcg tttttgcagt aattgttcat ggcgacgttt ttcgccaatc atcacaacaa
     1501 tcaacaagat aaccgccaga atgctgccgc caatcattac cataaagccg ccatcccagc
     1561 cgaagaagtc cacggtgtag ccaacaatcg cgctcgccgc caccgaaccg cccaggtaac
     1621 caaacagccc ggtaaagccc gctgccgtac ctgccgcttt tttcggtgcc agttccagcg
     1681 catgcagacc gatcagcatc acaggaccgt agatcaggaa gccgataaca atcatacaaa
     1741 tcatatcgac ggttgggtta cctgccgggt tcatccagta aacgatagtc gcgatggtca
     1801 ccagtgtcat aaagaaaacg ccggttgccc cacggttgcc acggaagact ttatccgaca
     1861 tccagccgca cagcagagtg cccggaatac ctgcatattc ataaaggaag taggcccagg
     1921 aggatttatc cagcgcgaaa tgcttaacct ctttcagata agtcggtgac cagtcgagga
     1981 tgccgtaacg cagcagataa acgaacacgt tggcgatggc gatataccac agcagtttgt
     2041 tcggcagtac gtactgcatg aagatttgct tcgccgtcag ctcctgttcc gctttttcgt
     2101 tatagtcgtc cggataatca tttttgtact cttcgatcgg cggcaagcca caggattgcg
     2161 gggtatcgcg catcatcgca aaggcgaata atgccaccag aatggcgcag aaagcaggca
     2221 tatagagcgc cgcatgccag tcattgaacc aggccatccc cagcaggaac agcagcggcg
     2281 gaataccacc accgacgttg tgcgcacagt tccacactga cacaatgccg ccacgttctt
     2341 tctgcgacca ccagtgcacc atagtacgac cacacggcgg ccaccccatc ccctggaacc
     2401 aaccgcagag gaacaacagt acaaacatca ccgcaatgct cgacgtcgcc catggcacaa
     2461 agcccataaa caacatcact gccgccgcca gaatcaaacc tgcgggcagg aaaacgcgcg
     2521 gattcgagcg atccgatacc gaacccatga tgaatttcga aaatccataa gcaatcgaga
     2581 tccccgaaag ggcaaaacct aaatcaccgc gtgagaatcc ctgctcaacc agataaggca
     2641 tagcaagcgc aaagttctta cgaaccaaat agtaagccgc atagccaaag aatatcccca
     2701 ggaaaatttg ccagcgcaat cgacgataag tcggatcgat ctccgcggca ggtaagcgcg
     2761 ctttgtgtgg cgctggttta aaaatactca acattgatag cctccgtggc ccgtggtctt
     2821 atttatgatt aacagcctga ttcagtgaga gaacctgccg tttcttgagt tgccgcgatg
     2881 ttaagaaaac attcataaat taaatgtgaa ttgccgcaca cattattaaa taagatttac
     2941 aaaatgttca aaatgacgca tgaaatcacg tttcactttc gaatcatgag cgaatatgcg
     3001 cgaaatcaaa caattcatgt ttttactatg gctaaatggt aaaaaacgaa cttcagaggg
     3061 ataacaatga aaactcgcga ctcgcaatca agtgacgtga ttatcattgg cggcggcgca
     3121 acgggagccg ggattgcccg cgactgtgcc ctgcgcgggc tgcgcgtgat tttggttgag
     3181 cgccacgaca tcgcaaccgg tgccaccggg cgtaaccacg gcctgctaca cagcggtgcg
     3241 cgctatgcgg taaccgatgc ggaatcggcc cgcgaatgca ttagtgaaaa ccagatcctg
     3301 aaacgcattg cacgtcactg cgttgaacca accaacggcc tgtttattac cctgccggaa
     3361 gatgacctct ccttccaggc cacttttatt cgcgcctgcg aagaagcagg gatcagcgca
     3421 gaagctatag acccgcagca agcgcgcatt atcgaacctg ccgttaaccc ggcattgatt
     3481 ggcgcggtga aagttccgga tggcaccgtt gatccatttc gtctgaccgc agcaaacatg
     3541 ctggatgcca aagaacacgg tgccgttatc cttaccgctc atgaagtcac gggactgatt
     3601 cgtgaaggcg cgacggtgtg cggtgttcgt gtacgtaacc atctcaccgg cgaaactcag
     3661 gcccttcatg cgcctgtcgt ggttaatgcc gccgggatct gggggcaaca cattgccgaa
     3721 tatgccgatc tgcgcattcg catgttcccg gcgaaaggat cgctgctgat catggatcac
     3781 cgcattaacc agcatgtgat caaccgctgc cgtaaacctt ccgacgccga tattctggtg
     3841 cctggcgata ccatttcgct gattggcacc acctctttac gtattgatta caacgagatt
     3901 gacgataatc gagtgacggc agaagaggtt gatattctgc tgcgtgaagg ggaaaaactg
     3961 gcccccgtga tggcgaaaac gcgcattttg cgggcctatt ctggcgtgcg cccgctggtt
     4021 gccagcgatg acgacccgag cggacgtaac gtcagccgtg gcatcgtgct gctcgaccat
     4081 gctgaacgcg atggtctgga cggatttatc accatcaccg gtggcaaact gatgacctat
     4141 cggctgatgg ctgaatgggc taccgacgcc gtgtgccgca aactgggcaa cacgcgcccc
     4201 tgtacgaccg ccgatctggc acttcctggt tcacaggaac ccgctgaagt taccttgcgt
     4261 aaagtcatct ccctgcctgc cccgctgcgc ggttctgcgg tttatcgtca tggcgatcgc
     4321 acgcctgcct ggctgagcga aggtcgtctg caccgtagcc tggtatgtga gtgcgaagcg
     4381 gtaacagcgg gtgaagtgca gtacgcggta gaaaatttaa acgttaatag cctgctggat
     4441 ttacgccgtc gtacccgtgt ggggatgggt acctgccagg gcgaactctg cgcctgccgc
     4501 gctgccggac tgctgcaacg ttttaacgtc acgacgtccg cgcaatctat cgagcaactt
     4561 tccaccttcc ttaacgaacg ctggaaaggc gtgcaaccca tcgcctgggg agatgcactg
     4621 cgcgaaagcg aatttacccg ctgggtttat cagggactgt gtggtctgga gaaggagcag
     4681 aaagatgcgc tttgatactg tcattatggg cggcggcctc gccggattac tctgtggcct
     4741 gcaactgcaa aaacacggcc tgcgctgtgc cattgtcacg cgtggtcaaa gcgcgctgca
     4801 tttctcatcc ggttcgctgg atttgctgag ccatctgcct gatggtcaac cggtgacaga
     4861 cattcacagt ggactggaat ctttgcgtca gcaggcacca gcccatcctt acagtcttct
     4921 cgaaccacaa cgcgtgctcg atctcgcttg ccaggcgcag gcattgatcg ctgaaagcgg
     4981 tgcgcaattg cagggcagcg tggaactggc ccatcagcgc gttaccccgc tcggcacttt
     5041 gcgtgcaacc tggttaagtt cgccagaagt tcccgtctgg ccgctacccg cgaagaagat
     5101 atgtgtagtg gggataagcg ggctgatgga tttccaggcg catcttgcgg cggcttcgtt
     5161 gcgcgaactg gatctttcgg ttgaaaccgc agaaatagag ctgccggaac tggatgtcct
     5221 gcgcaataac gccaccgaat ttcgcgcggt gaatatcgcc cgtttccttg ataatgaaga
     5281 aaactggccg ctgttacttg atgcgcttat tcctgtcgcc aatacctgcg aaatgatcct
     5341 gatgcccgcc tgcttcggcc tggccgatga caaactgtgg cgttggttga atgaaaaact
     5401 accttgttca ctgatgcttt tgccaacgct gccgccttcc gtgctgggca ttcgtctgca
     5461 aaaccagtta cagcgccagt ttgtgcgcca gggtggcgtg tggatgccgg gcgatgaagt
     5521 gaaaaaagtg acctgtaaaa atggcgtagt gaatgaaatc tggacccgca atcacgccga
     5581 tattccgcta cgtccacgtt tcgcggttct cgccagcggc agtttcttta gtggcggact
     5641 ggtagcggaa cgtaacggca ttcgcgagcc gattctcggc cttgatgtgc tacaaaccgc
     5701 cacgcggggt gaatggtata agggcgattt ttttgcgccg caaccgtggc agcagttcgg
     5761 tgtaaccact gatgagacgc tacgcccgtc gcaggcaggg caaaccattg agaacctgtt
     5821 tgccatcggt tcggtgctgg gcggatttga tcccatcgcc cagggatgcg gcggcggtgt
     5881 ttgtgccgtc agtgctttac atgccgctca acagattgcc caacgcgcag gaggccaaca
     5941 atgaacgaca ccagcttcga aaactgcatt aagtgcaccg tctgcaccac cgcctgcccg
     6001 gtgagccggg tgaatcccgg ttatccaggg ccaaaacaag ccgggccgga tggcgagcgt
     6061 ctgcgtttga aagatggcgc actgtatgac gaggcgctga aatattgcat caactgcaaa
     6121 cgttgtgaag tcgcctgccc gtccgatgtg aagattggcg atattatcca gcgcgcgcgg
     6181 gcaaaatatg acaccacgcg cccgtcgctg cgtaattttg tgttgagtca taccgacctg
     6241 atgggtagcg tttccacgcc gttcgcacca atcgtcaaca ccgctacctc gctgaaaccg
     6301 gtgcggcagc tgcttgatgc ggcgttaaaa atcgatcatc gtcgcacgct accgaaatac
     6361 tccttcggca cgttccgtcg ctggtatcgc agcatagcgg ctcagcaagc acaatataaa
     6421 gaccaggtcg ctttctttca cggctgcttc gttaactaca accatccgca gttaggtaaa
     6481 gatttaatta aagtgctcaa cgcaatgggt atcggtgtac aactgctcag caaagaaaaa
     6541 tgctgcggcg taccgctaat cgccaacggc tttaccgata aagcacgcaa acaggcaatt
     6601 acgaatgtag agtcgatccg cgaagctgtg ggagtaaaag gcattccggt gattgccacc
     6661 tcctcaacct gtacatttgc cctgcgcgac gaatacccgg aagtgctgaa tgtcgacaac
     6721 aaaggcttgc gcgatcatat cgaactggca acccgctggc tgtggcgcaa gctggacgaa
     6781 ggcaaaacgt taccgctgaa accgctgccg ctgaaagtgg tttatcacac tccgtgccat
     6841 atggaaaaaa tgggctggac gctctacacc ctggagctgt tgcgtaaaat cccggggctt
     6901 gagttaacgg tgctggattc ccagtgctgc ggtattgcgg gtacttacgg tttcaaaaaa
     6961 gagaactacc ccacctcaca agccatcggc gcaccactgt tccgccagat agaagaaagc
     7021 ggcgcagatc tggtggtcac cgactgcgaa acctgtaaat ggcagattga gatgtccaca
     7081 agtcttcgct gcgaacatcc gattacgcta ctggcccagg cgctggctta aactcctttc
     7141 tgatgcccgg taagcatgtg gttaccgggc atttttgcgt acacgattcc gtgcccaatg
     7201 tatgcgttgc aacgcagtga aaattcctct gaaaacgtct cgcaaaggct gaaactggca
     7261 gatgtcaaag gcctgggata accgtaatgt cgcgtcatca taaatatcag gtgacggaca
     7321 accatgaccg aatcaacaac ctcctccccg catgatgcgg tatttaaaac ctttatgttc
     7381 acacccgaaa ccgcacggga ttttctcgaa atacatttgc cagaaccact gcgcaagctt
     7441 tgcaacctgc aaaccttacg cctggaaccc actagtttta ttgaaaaaag tttacgcgct
     7501 tactactcgg atgttttgtg gtccgtggaa accagcgacg gtgacggcta tatctactgc
     7561 gtgattgaac atcaaagctc tgcagaaaag aatatggctt ttcggctaat gcgctatgcc
     7621 actgccgcca tgcagcgtca cctggataaa ggctatgaca gagttccgct ggtggtgcca
     7681 ttgctgtttt atcatggcga aacatcgccc tacccgtact cacttaactg gctggatgag
     7741 tttgacgatc cgcaacttgc ccggcagttg tacaccgaag cttttccgtt ggtggatatc
     7801 accatcgtac ctgacgatga gatcatgcaa catcggcgta tagctctgct tgaactgatt
     7861 caaaagcata ttcgcgaccg cgatttaatc ggtatggtcg acaggatcac cacgcttttg
     7921 gttaaaggct tcactaatga cagccagcta caaacactgt ttaattatct gctgcaatgc
     7981 ggcgatacct cccgtttcac ccgttttatt gaggagattt ccgaacgttc accactacaa
     8041 aaggagagat tgatgactat tgctgagcgg ctacggcagg aaggacacca aattggctgg
     8101 caggaaggta tgcatgaaca agccattaaa attgctttgc gcatgctgga acagggcatt
     8161 gatcgtgacc aggtgctcgc ggccacccag ctaagcgaag ccgatctggc agcgaataac
     8221 cactaattaa cacaggccca cagccgatcc ccatgggcct ttgatatcaa taactacctt
     8281 ttatgcgtgg cccatttttg cctgatttaa acatcgccag tcgttgatcc agggcatcgc
     8341 tgtagagcat cgtgtcaacg ccaacagcga caaagttcgc tccccacgcc aggcattgct
     8401 gcgccatatc aggagccaca gccagaaaac cagccgcttt acccgcagca cggatccgcc
     8461 gaatgctggt ttcaataatt cgctgcactt ccgggtgccc ggcgttatcc gggtagccca
     8521 acgacgcaga aagatccgct ggtccaataa acacgccatc aatcccttcg acgtcgagga
     8581 tctcgtccag gttatccagt gccgttttac tttccacctg cactaacaga caaagcgaat
     8641 cgttgacttg cgccatgtaa ttctcaatgc gtccccagcg cgcagcccgt gccacactgg
     8701 ccccaacacc acgctcaccg tagggaggat agcgcgtggc agacgccacc tgacgagcct
     8761 gatcggcagt atcgaccatc gggatcaata gagtttgcgc gccaatatcc agaacttgtt
     8821 taatcagcga tttactgcct tccaccggac ggatcacggg ttggctggca tagggcgcta
     8881 ccgcctgtag ctggtgataa agatcctgaa tggtgtttgg cgcgtgctcc ccgtcaatca
     8941 gcaaccagtc ataaccggaa gtagcggcaa tttctgccat ataagaagta gtcgagctta
     9001 accacagacc aatttgcact tcgcccttgc gtaaacgttc tttaaaggga ttggttaata
     9061 atgcgttcat cgtgttcctt aacctattta atgatgtgcc acgtcggtct gcgcaacagt
     9121 acgatttaca cgcagagtga aaataatcag cgaaccgatg accgccaccg ctgccagtgt
     9181 cagtaatcct gccgcatcgc tggcaaataa cgtttctgct ttcacgcgca ggatcggggc
     9241 aataaacccg cccactgcac caaacaggtt tacaaaacca atccccgcag ccagcgccgt
     9301 accggaaagc agttgtgtcg gcatcgtcca gaacaccggc tgcacggcaa taaacccgat
     9361 agctgcaaca cacagcgcta cgatcgccat tactggagaa agcagcccgg acagaccaat
     9421 accaatgccc gccgccagta atgtcagcgc tgcgacatta cgccgttcgc cggttttatc
     9481 ggaatagcgc ggaataagcc aggtcccaaa caaggccgca acccacggaa tggcggtgac
     9541 caccgacgct gtaaagccca cttttgttcc cagcaatgcc gcaacctggg tcggcaggaa
     9601 gaaaattaat ccgtaaaccg ccacctgaat ggtcaggtag ataatcgcta gttgccagac
     9661 tcggccatta cgcagcgcat cgctcagccg agaagtcact ttctgttgtt cttcacttgc
     9721 cagttgattg ataagcaacg ttttttcttg tttactcaga aaacgtgcct gctccggtgt
     9781 gtcatcaagc caaaagaatg tgaatacccc agcaccgact gccaacaatc cttcaatcac
     9841 aaacatccag aaccagccgg gatgccccat aaatccatgc atctccaaca gcgcgccgga
     9901 aagcggtgat cccagtgtta acgccagcgg tgcgcccata tagaacagcc ccataatgct
     9961 ggcgcgatta cgctgcggaa accattgcga ggtgagataa atcataccag ggaaaaatcc
    10021 ggcctccgca gcaccaagca gagtgcgaac tatcagaaat ttcgcttcag tatcggccca
    10081 tgccatggct gccgaaagaa atccccacag cagtgttgtc gtaccaatcc aggttctggc
    10141 ccccagtttg cgcatcaaaa gattcgccgg aacacccaga aacgcatata ccacaaagaa
    10201 aatgcctgct cccagcgcat aagcttcatt actcaacccg gtatcaatct ggtaggtctg
    10261 tttggcaaaa ccgatattcg aacggtcgag aaacgccagc acatacagcg ccaacataaa
    10321 cggaattaaa cgcacacggt ttttcttcac cacggcgtca agcaaagcgg tgctcataaa
    10381 aagctcctta gaatatgtgg gcgttaccgt tgtcacagca acgcccggag tgattagtgg
    10441 ctgtaggggc gtttcaaatt gcagtcacga ttgagttcga cgccaaaacc gggtttatca
    10501 agcactgatt tatgaatacg accattcacc ggaaccggct cattgagcag aatcgggtca
    10561 aactgcggac gcatcgttga acaatccggg ctggtcatca ggaattcgct gaatggcgta
    10621 ttggtgaagg tgatcaccgc atggtgagag taaacagacg aaccgtgcgg caccaccagt
    10681 tgccccctgg atttggcgat tgcggcaatt tccaccagcg tggttaaacc accgcaccag
    10741 ccaacatccg gctgcataat gtcgataccg gtttctgaaa gcgtacgaaa agattgcagt
    10801 gtgccgtggt gctcaccgct ggtgaccatc attccgactg gcgcgttgcg tttcagttcg
    10861 cgataacctt catactgctg tggcggcagg cactcttcga tccatttcag gttatagggc
    10921 gcgcaagcgt gggccagttt ggtggcatag ttcacgtcct gactcatcca gcagtcgagc
    10981 attaaccaga aatcctcacc gcatttttca cgcatatccg cgaccataac ggcatctttg
    11041 cggatccccg catcgccatc atgtggcccc cagtgcgtcg gcattttgcc accgataaag
    11101 cccatctctt ttgccagatc cggacgcgcg cctgtggcgt agaactgaat ctcatcacga
    11161 actgcgccgc ctaaaagttt ataaaccgga agcccgacca ctttgccgaa cagatcccac
    11221 agagccagat cgacacagga aatcgtgttc atcaccaggc caccagagcc ggagtaatac
    11281 agggtggcat tgagcatttg atcatggatc agtttgatat cactgacaca tttaccctca
    11341 atgaaacggt taagatgttt ttcgacaata aaacagccca tttcaccggc tgtcgaaaca
    11401 gcgaatccgg tctggccgtt ttctgcttcg acttcaacaa tcaacgtgcc aagaacgtta
    11461 atgccaaatg actggcgtga ctgctcgtaa tcgcggtatt tactcatcgg ggtggcaata
    11521 tgatcgtcaa tccagtgatt cgccccctgg tcgtgataat caccgccgcc agcacctttt
    11581 tctgctgtcg caccgccagt aaaccaggcg cgaacctgtt tgatttttgg tagggtcatg
    11641 atgttctcca ttgttatgag gcctgtaagt caaagggact tttccatccc aacagacgtg
    11701 aaatatccct ggcgcaggca atggccttgc ccgccagata atcacggtat tcttcattga
    11761 tttgtaagcg ggtaccgacc accgagatcg cagcggtaag ctcgttattg gcgttaaaca
    11821 ccggcgcagc gacacaacgg acatcggggt aatcttcgcc gttgtcatag ctccagccct
    11881 gacggcgaat acgcgccagt tcttcgtgaa gttgctgtgg atgagtaatc gttgtgggtg
    11941 tcgcctgctc ccagaccagc ccttcgataa tactttgctg taccgctgca ggttgccagg
    12001 caagcaggca tttacctatt ccggagcgat aaagcgaaag gctttttcct tcatgggaac
    12061 gcacgctgat agtggctgat gactccactt ttaaaatgta ataagcgctg ccgttatcaa
    12121 taatccccag gtgacataac agcccggtgg tatccatcag ttgcgtcaaa cgcggtcgag
    12181 ccagttcccg gagatccatc ttgcttaacg catgaccaga aagctccacc agcctggtcc
    12241 acaaacaaaa attttcctgg tgatcaaggc taagaaaacg ctgacgcctg agttcattaa
    12301 gcagcaaata ggccgtgctt ttagggattc ccaatgtgtc aatgatcgtc gcagcactac
    12361 aggggccaat gcgggcaatc aaattgagaa tatcaatagc ccgtgtcagg gcgggaactt
    12421 tgcttgattc caacatactg gactccagtc ttagatactg gaatcagtct cgttgctcag
    12481 ggggagtaaa attgtgaagc gcatcaaatc ccgctcctcg tccagcggac tggagaaatt
    12541 acgcacaaat ttgtgaatca gtgcacggat tttttcttat tgagtacaac aggttggaca
    12601 agttttgccg gagggggaat gtgccggtgc agtaaaccgg cacagagtgg cggaatcaga
    12661 cagataaggt catggactca acaacctcaa tccagccatg ctcactggcg atatcctggc
    12721 cgtttaacca acggcgcagc atattcagtg ccatcattgc gcacacttcc tgacggatag
    12781 ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc gaaagtgccg tctggcgtgg
    12841 ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac agccagtgcc agcccggcaa
    12901 aatggttggc ccgccgttct gtaatccagt gcgcagtttg cgccagggtt tcttcctgtg
    12961 aaggaaccac ttcacacgcc agcaatggag cacctgcgcg agaaagttgc aaagccaata
    13021 aaccaccggt gaattgctcg ctcaacgtca ggctgaactg gcggttttgc aattcgcgac
    13081 tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac gctctgtccg gcaacacgtt
    13141 taacgtccag ccacagtttt tccatcgcct gttgctcgct tgccggtccg gtgagtttca
    13201 gttcgatgat cggcattgag gagcgatagc ccattgttac acccggcggc agttgtagag
    13261 tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa ggtagttaaa cgcagacaaa
    13321 ccggcgactg tggtaaagaa aaacgctcgc gcaggcgcgg caggatttcg tgctcgacca
    13381 tcaccttaaa ttctgacggt acgccgggag taaagaacat caggcaacga ttaagccgca
    13441 cggcaaaacc acaggcggtg cctaccgggt tattgataaa ttcagcactg gcaggcagct
    13501 ccgcttgttt acggttgctc ggtgccatta ctcgtccacg ttcgtggaaa tagcgttcca
    13561 tctctttgag ccaggcttca tgcagcacca ggccttcacc ttttgctgtc gcagcggcga
    13621 gtgcacttaa atcatcactg gtcggtccca gcccgccgtt aacgatcagc acatcggcgt
    13681 gctgactacg ttcgcgaaga atggtgacta agtcatcaag gttatccccc accgtattgc
    13741 ggcgagataa tggcaacccc tgatgaaaga aaaaatcggc cagccaggca gcgttagtgt
    13801 caacgatttg cccgtgtaac acttcatccc cggtggataa catttccact tttaacatgg
    13861 ttatctcctg cttttgttat cgaatcacta taacgcaaag ggagataagg cgtagtaaga
    13921 aactgaatgg cgcgacagaa tgccgcgccg gaagggatca gaaactggcg ttaaccccaa
    13981 catacgggcc gtcagccacg gcgttgtcgc ggttaccgtc tttacccgac agattcaggt
    14041 agcgataacc cgcctcaata ctgactggat gcataatggt gtaacgcgcg ccagcattcg
    14101 cttcctcata acttttaata ccgctggaga gtgaatccgg agagtagtaa tactcgccaa
    14161 acaaacggaa gctgttgcca attttccact gcaaaccacc tcctaccgct gcggcataac
    14221 cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc gccaacggtc gccattaacg
    14281 gcccgagagg cagattcaac cccagcccca cgccagcgac gtcaccgtcg tcgtcgttat
    14341 gtgtccagtt accgcttaac gccaggcccg tcgattcagt cccaaaaccg acaccaatat
    14401 tggtgtattc tttacccgcc tgaccgctga tgctcattgc attgaccgat gcagaaacca
    14461 acagcattcc ggccagacct gctagcgcaa tttttttcat tatccccttt cccacaagca
    14521 attaatcaaa attcccaaag ctgcgggatt gtactgcctg gcacagagga ttcaatgagc
    14581 gaaaaaaggg atctagtcac ttattgtaac taaatacttc ttaaataatt aaacgccgaa
    14641 aaaataaaca cactttaccg atacacacaa aaataataat aagtgcagtt atcatcgcaa
    14701 aagagattac tgtcactttc acatatttct gagttaccgt attctcatgg tttcttttta
    14761 tttataagga atacccctgt gcgacaacgg actattgtat gccctttgat tcaaaatgat
    14821 ggtgcttatt tgctgtgcaa aatggccgac gatcgcggcg ttttccccgg tcaatgggcg
    14881 ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag aggcactacg ccgcgaaatt
    14941 cgcgaagaac tgggagaaca gctgcttttg acagaaatca cgccgtggac cttcagcgat
    15001 gatattcgca ccaagacgta tgcagatggt cgcaaggaag agatttatat gatttacctg
    15061 atttttgact gcgtttctgc caaccgagaa gtgaaaataa acgaagagtt tcaggactac
    15121 gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa atgttgccac ccgaaaaacg
    15181 ttacgtttga aaggtcttct ttaacaatga tgacagccat cactggctgt cagattgaaa
    15241 atttagtggt taacgaattc cccatccaga taaactttgc ctttttccac atgcatgact
    15301 aaaccatcca gataatcagg tttaaatgtc gcatcacgct tatttttagc aatatatgtc
    15361 aggcaatgat tatgggtgaa aataacgata tttttatcag gcgctttgct ttgtaagtcc
    15421 ttaattgcac tataaatctc attaccgcac tgaagaagtc gtttatctac cgtcagtttt
    15481 ttacccgcgg aaaaccaggt cgccgactga atggtccgga cggtattact ggaataaaga
    15541 tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt cacgggcatc ctgggtacct
    15601 ttaaccgtaa tacctgtttt atctgacaag cattgattgg ttgaacgatc gcaacgttca
    15661 gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca gtctggccaa tgttttattg
    15721 tcgatacgtg gcaaaccatt gctactccag gcggcatgag tacccagtcc ggcaattgca
    15781 gcgagcgcca gtaaaatgat gaaatatttt tttgacttca atgatgagcg gcaaaaagct
    15841 aacacgtaaa ctccacctat agacaagcgc aaccagacaa ttaccgtgaa attgagcgac
    15901 atttctggcg ataattagca gttggtgtaa tattaaaaat cctacgatgt cggcaaaatg
    15961 cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa atataaaaat ttcaatattt
    16021 acgtctaata ttagtttctt aaggttaagt taatattcta tccttaaaat ttcgctccaa
    16081 atggcaaaat atacacaaca ctctttatag caaatataag tggacaggta ttcaatggcg
    16141 gaaggaaaag caatgtcaga atttttgcct ttttcgcgac cagcaatggg cgtggaggaa
    16201 ctcgctgcag ttaaagaggt tctcgaatcc ggttggatca caaccggtcc gaagaatcag
    16261 gcgcttgagc aagccttttg ccagttgacg ggaaatcagc atgccatcgc ggtcagttca
    16321 gccaccgccg gaatgcatat cacgctaatg gcgttggaaa ttggcaaggg cgatgaagtg
    16381 attacgcctt ccctgacctg ggtttcaacc ctcaatatga tttccttgtt gggtgcaacg
    16441 ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca cgcctgaagc gatcgagtca
    16501 gccattacgc cacgcactaa agccatcatt ccggtgcatt atgccggtgc gccagcagat
    16561 attgacgcca ttcgcgccat tggcgaacgt tacggcatcg cagttatcga agatgctgcc
    16621 catgccgtcg gtacgtatta caaagggcga catattggcg caaaaggtac cgctattttt
    16681 tcatttcatg ccattaaaaa tattacctgt gcggaaggtg gcctgattgt aactgataat
    16741 gaaaaccttg cccgccagct acggatgctg aaatttcacg gtctgggtgt cgatgcctat
    16801 gacagacaaa cctggggccg tgcaccgcag gctgaagtct taacaccggg ctataagtac
    16861 aatctgaccg atattaacgc cgcgattgcc ctgacacagt tagcaaaatt agagcacctc
    16921 aacacccgtc ggcgcgaaat tgcccagcaa tatcagcaag cactggcagc tctccccttt
    16981 cagccattaa gccttcccgc ctggccgcac gttcacgcct ggcatctgtt tattattcgt
    17041 gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga tggaagcgtt aaaagaaaga
    17101 ggcattggta ccgggttaca tttccgcgcc gctcacacac aaaaatatta tcgcgagcgt
    17161 tttcccacgc tgtcgttacc gaataccgaa tggaatagcg aacgcatctg ttctttgccg
    17221 ctgttcccgg atatgactac cgccgatgcc gaccgtgtca tcacagccct tcagcaactc
    17281 gcaggacaat aagccatgtt tgaaatccac cctgttaaga aagtctcggt ggttattccc
    17341 gtttataacg agcaggaaag cttaccggaa ttaatcaggc gcaccaccac agcctgtgaa
    17401 tcgttgggga aagagtatga gatcctgctg attgatgacg gcagtagcga taattccgcg
    17461 catatgctgg tcgaagcctc acaagcggag aacagccata ttgtgtctat tttgcttaac
    17521 cgcaattacg ggcaacattc agcgattatg gcgggattca gtcacgttac tggcgactta
    17581 attattaccc ttgatgccga tctccagaat ccgccagaag aaatcccccg cctggtggca
    17641 aaagccgatg aaggttacga cgtggtaggg actgtacgcc agaaccgcca ggacagctgg
    17701 tttcgtaaaa cagcttcgaa gatgattaac aggcttattc agcgcaccac cggcaaagcg
    17761 atgggtgact acggttgtat gctgcgcgcc tatcgccgtc atattgtcga tgcgatgttg
    17821 cactgccatg aacgtagcac ctttatcccg attctggcga atatcttcgc ccgccgtgcc
    17881 attgaaattc cagtacatca tgccgagcgt gagtttggtg aatccaaata cagttttatg
    17941 cgcctgatta atttgatgta cgacctggtg acctgcctta ccacaacgcc gctacgtatg
    18001 ctgagtctgc tcggcagcat tattgcgatt ggaggtttta gcattgcggt gctgctggtg
    18061 attttacgcc tgaccttcgg accacaatgg gcggcagaag gcgtctttat gctatttgcc
    18121 gtgctgttta cttttattgg cgctcagttt atcggcatgg gattgctcgg tgaatatatc
    18181 ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt ttgttcagca agttatccgt
    18241 ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt tgcctaccac gatatgggat
    18301 gcctcggtat tgaagctctg ctggctgccg gttacgaaat tagcgccatt tttacccata
    18361 ccgataatcc cgctgaaaaa gccttttatg gttcggtggc tcgtctggcg gcggaaagag
    18421 gcattccagt ttatgcgccg gataacgtta atcatccgct gtgggtggaa cgcattgccc
    18481 aactctcacc agaggtgatt ttctcttttt attatcgcca tcttatttgc gacgagattt
    18541 tgcagctcgc tcccgcaggt gcatttaatc tgcacggttc gctgttacca aaatatcgtg
    18601 gtcgcgcgcc gctgaactgg gtgctggtga acggtgaaac ggaaacgggc gttacattgc
    18661 accgaatggt aaaacgtgcc gatgctgggg ccattgtggc gcaactgcgc gttgccattg
    18721 cgccagacga tatcgctatt acgctgcatc ataaattgtg ccatgccgcg cgccagctac
    18781 tggaacagac attacccgcc attaaacacg gtaatattct ggaaattgcc cagcgcgaaa
    18841 acgaagccac ctgttttggt cacagaacgc cagatgacag tttccttgaa tggcataaac
    18901 cggcatccgt actgcacaac atggtacgtg ccgttgccga tccgtggccg ggtgccttca
    18961 gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg tgttcatcct catgccagca
    19021 aagcacagcc ggggagcgtg atttctgttg cgccactgct gattgcctgt ggcgatggcg
    19081 cgctggaaat cgtcaccgga caggcgggcg acggcattac tatgcagggc tcgcaattag
    19141 cgcagatgct gggcctggtg caaggttcac gcttgaatag ccagcctgcc tgcaccgccc
    19201 gacgccgtac ccgggtactc atcctcgggg tgaatggctt tattggcaac catctgacag
    19261 aacgcctgct gcgcgaagat cattatgaag tttacggtct ggatattggc agcgatgcga
    19321 taagccgttt tctgaatcat ccgcattttc actttgtcga aggcgatatc agtattcatt
    19381 ccgaatggat tgagtatcac gtcaaaaaat gtgatgtcgt cttgccgctg gtggcgatag
    19441 ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt tgaactcgat tttgaagaga
    19501 atctgcgcat tatccgctac tgcgttaagt accgtaagcg aatcatcttc ccgtcaactt
    19561 cagaagttta tgggatgtgt agcgataaat acttcgatga ggaccattct aatttaatcg
    19621 tcggcccggt gaataaacca cgctggattt attcggtgtc aaaacaatta cttgatcggg
    19681 tgatctgggc ctatggcgaa aaagagggtt tacagttcac cctcttccgc ccgtttaact
    19741 ggatgggacc acgactggat aaccttaatg cagcgcgaat tggcagctcc cgcgctatta
    19801 cgcaactcat tctcaatctg gtagaaggtt caccaattaa gctaattgat ggcggaaaac
    19861 aaaaacgctg ctttactgat atccgggacg gtatcgaggc gttgtaccgc attatcgaaa
    19921 atgcgggaaa tcgctgcgac ggcgaaatta tcaacattgg caatcctgag aacgaagcga
    19981 gcattgagga actgggcgag atgctgctgg cgagcttcga aaaacatccg ctgcgccatc
    20041 acttcccacc gtttgcgggc tttcgtgttg tcgaaagtag cagctactac ggcaaaggat
    20101 atcaggacgt agagcatcgt aaaccgagca tccgcaatgc ccgccgctgc ctggactggg
    20161 aaccgaaaat tgatatgcag gaaaccatcg acgaaacgct ggatttcttc ctgcgcaccg
    20221 ttgatcttac ggataaacca tcatgaccaa agtcggctta cgcattgatg tcgatacctt
    20281 tcgtggcacc cgtgaaggcg tgccacgtct gctggaaatc ttgagtaagc ataatattca
    20341 ggccagcatt tttttcagcg tcggcccgga caatatgggc cgccatctct ggcgactggt
    20401 gaagccacag tttttgtgga agatgctgcg ctcaaacgcg gcatcgcttt atggctggga
    20461 tattttactg gcaggtacgg cctggccagg taaagagatt ggtcatgcca atgccgatat
    20521 cattcgtgaa gcggctaaac atcacgaagt cggcctgcac gcctgggatc accatgcctg
    20581 gcaagcccgt agcggtaact gggatcggca aacaatgatc gacgatattg cacgcggtct
    20641 tcgcactctg gaagagatta tcggtcaacc ggtaacctgt tctgccgctg cgggctggcg
    20701 tgccaaccag aaggtgatcg aagcaaaaga agcgttccat ttgcgctaca acagcgattg
    20761 tcgtggggcc atgccgttcc gtccactgct cgaatcagga aaccctggca ctgcgcaaat
    20821 tccggtgacc ttacccacct gggatgaagt gattggtcgg gatgtgaaag cagaagattt
    20881 taacggttgg ttactcaacc gcatcctgcg agataaaggc acgccggttt ataccattca
    20941 tgcagaagtt gaaggctgcg cttatcagca taattttgtg gatctcctca aacgcgcagc
    21001 tcaggaaggc gtgacatttt gccctttaag cgaactgtta tcagagacgt tgccgctcgg
    21061 acaagttgtt cgcggaaata ttgccggacg tgaaggctgg ctgggttgcc aacaaattgc
    21121 gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc tcttcgcgtt tattgcctgc
    21181 tattacctgt taccgatcag cacgcgtctg ctctggcaac ccgatgaaac gcgttatgcg
    21241 gaaatcagtc gagaaatgct ggcatccggc gactggattg tgccccatct gttagggcta
    21301 cgttatttcg aaaaacccat tgccggatac tggattaaca gcattgggca atggctattt
    21361 ggcgcgaata actttggtgt gcgggcaggc gttatctttg cgaccctgtt aactgccgcg
    21421 ctggtgacct ggtttactct gcgcttatgg cgcgataaac gtctggctct actcgccaca
    21481 gtaatttatc tctcattgtt tattgtctat gccatcggca cttatgccgt gctcgatccg
    21541 tttattgcat tctggctggt ggcgggaatg tgcagcttct ggctggcaat gcaggcacag
    21601 acgtggaaag gcaaaagcgc aggattttta ctgctgggaa tcacctgcgg catgggggtg
    21661 atgaccaaag gttttctcgc ccttgccgtg ccggtattaa gcgtgctgcc atgggtagcg
    21721 acgcaaaaac gctggaaaga tctctttatt tacggctggc tggcggttat cagttgcgta
    21781 ctgacggttc tcccctgggg actggcgata gcgcagcggg agcctgactt ctggcactat
    21841 tttttctggg ttgagcatat tcaacgcttt gcactggatg atgcccaaca tagagctccg
    21901 ttctggtact acgtgccggt catcattgcc ggtagcctgc cgtggctggg attactcccc
    21961 ggtgcactgt acacaggctg gaaaaaccgc aagcattccg caaccgtcta tttgttgagc
    22021 tggacgataa tgccgctgct gtttttctcc gtcgctaaag gtaaattgcc cacctatatt
    22081 ctttcctgct ttgcatctct ggcaatgctg atggcgcatt acgctttgct ggcagcaaaa
    22141 aataatcctc tggcgctgcg gattaatggc tggattaaca tcgcttttgg cttcactggc
    22201 attattgcca catttgtggt ctccccgtgg ggaccaatga acacgccggt gtggcaaacc
    22261 ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt cgctatgggc atttttcggc
    22321 tggtacacct taacaaacgt cgaaaagacc tggtcttttg ccgcgctttg cccgctgggg
    22381 ctggcgttgc tggtaggatt ttcaattcct gacagagtca tggaaggaaa acatccgcaa
    22441 ttttttgtcg agatgacaca agaatcactt cagccaagcc gctatattct tactgatagc
    22501 gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg atgacatcat catgtatcgc
    22561 cagacaggtg agttgaaata cggccttaat tatccggatg cgaaagggag atttgtcagc
    22621 ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg aggggattat tacactcgtg
    22681 ctttcggttg accgcgatga agatatcaac agtctcgcca ttccgcccgc agatgccatc
    22741 gatcgtcagg agcgtctggt gctgattcag tatcgtccca aatgatctgg ctaacattag
    22801 tctttgccag cttgcttagc gttgccgggc agttgtgtca gaaacaggca acctgctttg
    22861 tggcgataaa caaacggcgc aaacatatcg tgctgtggct gggactggcg ctggcttgtc
    22921 ttggtcttgc catggtgctc tggctgctgg tcttgcagaa cgtaccggta ggcattgcct
    22981 acccgatgtt aagtctgaat tttgtctggg tgacgctggc tgcagtaaaa ctgtggcacg
    23041 aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat tattggcggc attgtgatcc
    23101 tcgggagtac ggtgtaatgg gcctgatgtg gggattattc agcgtcatta ttgcctcagt
    23161 tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg ccgatgacgc acttatggga
    23221 ttttatcgcg gctctgttag cttttggcct ggatgcccgg atactgttac tcgggctgct
    23281 gggttatctg ctttccgttt tttgttggta taaaacgcta cataaacttg cgctaagcaa
    23341 agcctatgcc ttgctcagta tgagttatgt gctggtatgg attgcttcaa tggtacttcc
    23401 cggctgggaa gggacttttt cgctgaaagc actactggga gtagcctgta ttatgagcgg
    23461 gttgatgctg attttcctgc ccacgacaaa acaacgttac tgagttttcc ctgccacttt
    23521 acactgccgt tcccactcat cggggctata actgcttgcc gagaggactt tcaccgtatg
    23581 cagcttttca cgattaatac aataaagcgc attctgcaaa ggcgagagta aatcccccac
    23641 tttcacggcg aagtcactct tcacttcggc gatcattttt atcgcgccgc cagcatcgca
    23701 gagcataagt acatgtctgt tatcttgttt attgcagcac gattttttga ccagccattc
    23761 cattgcatta tcctgtttgc taagagtttt cacatcaatt tacagattaa cagataaacc
    23821 tattatcaac ggcaatggaa tggcaaaatt gcgaagtgaa actaatgatt tcttaatcct
    23881 ggcgttgcac ccactctttt aacgcctgac gtgaaatttt aataccgccg tttttcagct
    23941 ccggcggtaa agttagccag cgcaccggtt gttgaaaacg agccagctta tctttcaccc
    24001 attcgccaag atcaacggtt tgctggtcat actccaccac cgctaccggt cgatgaccaa
    24061 actccttgtc ggcaacgggg acgataaaca cctgcaaaac cgcaggatgt gcagcaatta
    24121 cgcgctcgac ttcttccggc tgaataccct ctccgccact gaagaacaga ttgtctaaac
    24181 gtccgacaat ggtcagcttg ccattatgca tctcaccgcg atcgcgcgta gcgtaccagc
    24241 cttcgtcatt aaccagtgaa accagttgcc cgttacgcca gtaaccttct gccatactgg
    24301 cagcccgcag ccacacttca ttattaacga ttttcacttc ccgacccggc agcggcgaac
    24361 caacgtctgc caggccgtcg gcttctttcg cacacaccgt ggaggcaaac tcggtcagac
    24421 catagccgca aaagcaacga atcccctgct cgcgcgcctg ttccgtcaac tcgaccggga
    24481 tagccgcgcc gccaagtaac accgctttca gggaaacgga actgcggtta accagcaaac
    24541 gccagagttg tgttggcacc agtgaagcgt gagtacagcc tgccagcatt tgctccaatg
    24601 gctgtttatc acgcaccgtc atccgcgcac cagcgtataa ccagcgccac ataattccct
    24661 gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc atcgtgatcg ccaaacggaa
    24721 tcagcgataa cacaccttgc gcactggcaa gatgggcctg ataagtatgg acagcggctt
    24781 tcggcaaacc ggtagaaccc gaggtcaacg tcattgagca cagacgcgtc ggctgccacg
    24841 tagcggcatg tgcgccttca acccgctgaa tgtgcagcga cgttaatgcc ggaaacgtgt
    24901 tttccccatc cggcaccagc gcaaattgca gcgtcagatt gggcagcaat tcttcaagca
    24961 acggttgcgg cagctgaggg ttcacgggca acacccgcgc cccgcattgc agtaacgcca
    25021 gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa catcacgccg ctgccctcaa
    25081 ccacaccctg cgccgcaaag cccgtagcta actcatcgac gcgagcacaa agctcgcgcc
    25141 agttgagttg ctcgtcatta agacgtaagg cgatggcttc tccccgcact tgccgccagt
    25201 gacgccacgg ccagtcagag aagatcataa caactgctcc agtacatcag cgtccactaa
    25261 cggcagtggg ctccccggcc agcgacgaac ctgctgcgcc tgcatcagat ccagcgtgtc
    25321 cagtcccgga atggtgtctg gcgttaacca ggcagcaatc cgcgccagtt gcgttagacc
    25381 taagctcgac tcaatggaag aactgatcac cgccgtcagc cccagcgcat gcgccgcctg
    25441 tacctgctcg cgtacttttt ccagactgcc cgtgagcgtg ggtttgataa ctaccgcgcg
    25501 cacgccctct tcagccacaa aggcaaaatc cggctcgcgc aggctttcat cccaggcaat
    25561 ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg cgggttttgc acggctcttc
    25621 gagaaacgcg atgcggtggc gataatccgg gttaacatat ttggcaaact gctgaccttt
    25681 cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc agatccggaa ttgcctccag
    25741 caacagatta accaccatgc cgtcgcgcac ggcttcgtac aatcccactt tgaccttcgc
    25801 cactttctcg cctggcatat cagcaagttt gaggatcaga tcgtcaggat cgccattaca
    25861 cagtggtgcc gcacggtaat tggccgcctg cgggagcgta tctgccagct ccgccaatgc
    25921 acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc tcgccatcgc ctgccagcca
    25981 gttatttacc caagcaagca gcacaatttg cgcctcttcc caggtttcct gactgaagcc
    26041 cggcagtggg gagatctccc cccacccttc gcgctcgcct tcacgcaggc aaacatacag
    26101 cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccatc cccgcgtcca tggggatctg
    26161 ccagcggtat acctgcgcgc tacgcattac ggattccgtt taaatttgct gaaatcaggc
    26221 tgacgtttct ggttgaaagc gttgcgacct tcctgacctt cttccgtcat gtagaacagc
    26281 atggtggcgt tgcccgccag ctcctgcaga cccgcctgac cgtcacagtc ggcgttcagc
    26341 gcagctttca ggcagcgcag cgccatcggg ctgttttgca gcatttcgcg gcaccaacgg
    26401 acggtttctt tttccagatc cgccagcggt accacggtgt tgacaaggcc catatccagc
    26461 gcctgttttg catcgtactg acggcacagg aaccagattt cacgcgcttt tttctgtccg
    26521 acgatgcgag ccatgtagga agcgccccag ccgccgtcga aggaaccgac tttcgggcca
    26581 gtctgaccga agatggcatt atctgccgcg atagtcaggt cgcacatcat gtgcagaacg
    26641 tgaccaccgc cgatggagta gccagccacc atcgcgacaa ccggtttcgg acaggtacgg
    26701 atctgacgct ggaagtccag cacgttcagg tgatgtacgc cggaatcatc tttatagccg
    26761 ccgtaatcac cacgcacttt ctggtcacca ccggagcaga acgctttatc gcctgcgcca
    26821 gtcagaatga tcacgccgac gttgtcgtca taacgcgcat ctgccagtgc ctggatcatc
    26881 tcttttaccg tcagaggacg gaaggcattg cgcacctgcg gacgattaat ggtgattttt
    26941 gcgataccgt cggtggattt ttcataacga atgtcctcaa aaccttcgga gcagtcgtgc
    27001 cattcaaccg gtgcgtaaag cattgcttca tcaggataaa tcatattgtg tcctttagtc
    27061 agaaacgcaa gatctgcgcc agacttgcga taacgccagc gggattttcc cgatgcgcgt
    27121 tatgtccggc gcgaggaatg acatggcagt cggcagccag ttccgccgcc aaggcgcgga
    27181 atttgctgtc acgttcacca cataaataat aaaacgcaaa tgtgcgggcg ctaaggttag
    27241 cacgtaaatc aggctggacg gcgagagaag tcgcctccag catggcggca agggttgcgc
    27301 cattattgtt actgcgcagc gccaccagct cccggcgttg atcgtcattg agcgaggcaa
    27361 aaacaggctg ttgataccag tcggcaaata ccgccgttaa cggttctgtg cgaaaacgct
    27421 gcgcccattg gcgatcggaa cgctgacgtt ccgcgcgttg ttcagcattt tgcaacccag
    27481 gatgcccgcc ttcgacaaca accccacaaa gccccggcat cccctggcaa gccgccatca
    27541 tcgccacccg tcccccaagc gagtacccca ccaaccagaa gttaaggatg ttgtaactaa
    27601 ccagggtttt acgcagtaag tcggtgacat catcaaatcc atcgacgcta atcgccgccg
    27661 aaccaccgtg acctgggaga taaacataca accgtgagta gtcggcaaac gcctcgccca
    27721 cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa aaacaccagc cagggtaaac
    27781 ctggtttgcc gtgttttgcc tgcgcgtgca ggatcataaa tggcttacct gcgccagaag
    27841 ttgctggagc gtttgcgcac catcggtgtc gttaaccacc atttcaatta ccgtggtggt
    27901 tggcgtgcgc caggcatcgg caagtgccgt ttcaagttcc tgccagtttt gcggacgatg
    27961 atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg acgttttgcg gcatcagata
    28021 gaaacgctcg cgctcgcttt tcggcgttgg cagcagcgag aaaatttgcc cgccgttgtt
    28081 gttcaccaca attaatatca gcggcgcgga aacctgacgc aataacgcca gcgcgttgag
    28141 atcgtaaagt gcggagagat cgcccacaat cgccagcgtc ggtttgccgc ttgcccgctg
    28201 aacgccggcg gcagtcgaga gcagcccgtc gataccgctg gccccacggt tgctgtacac
    28261 cgggtaacct gccggaagtt gcgaaagcgc atcaatcaga cgtaccacca ggctgttacc
    28321 gacaaacaat tgcccctgtt caggcagata gtcgctgatg cgatgcgcca gttgcgcttc
    28381 gccaaacgca tcgcgacggg caataaccgc ctgcattgcc tgttccgcca ggcgcgggat
    28441 ttcaacgcac cagggctggc gtttttctgc cggatgcagc tccagccagt cggcaatatt
    28501 ggcaattaag cggcgtccac gatggtgcgc cggatcaagc cgcccttcga tgtcatccac
    28561 aatccagtac tcttcgggtt cacagcttgc ctgccattgc aggagccgtt tgcccgtcag
    28621 gctgcttccc agttgcacca caatttgcgc ctgttgcagt tcgctggtcg ctttggcatt
    28681 gcctaaccag agatcggcac acggcagcgg ctgtccggtt tgcgacagca cgtcgccaat
    28741 cagcggccag ccaagagttt gcgcccacag tgcaactttt ttgccctctt ccgcactcat
    28801 gcgcccggca accaccacgc cgcgtttttg tcgccagaag aaccagtcgc gctgtttttc
    28861 actttccaga cgaggcgctt cacgcagcca cggtttgtcg tcctgccacc agtcgcccag
    28921 ccgctgttgc cagctaagcc cggtgtcgtc catttcgcca tacagcggtt cggcaaacgg
    28981 gcagttgata tggactccac cagaatgaag cgtaccgaga gcatggtcga tggtagaaac
    29041 cagccaacgt gcggggatat cctgggtcgg gcgcggcaat gaaatactgt gcgtggggtg
    29101 agaggcgaac attcccggct gacgaatcgc ctgattcgcg ccgcagtcaa tcagctccgg
    29161 cgggcgatcg gcggttaaca ggatcagttt ttctccggtt aacccagctt caatcagtgc
    29221 cggatagaga tttgctaccg ccgtaccgga ggtcacaatc accgccaccg gctgcttgct
    29281 gactttcgcc agccccagcg ccagatgccc caacccacgc tcatcgaaat gggtgtggtg
    29341 aatgaatgcg gaattctccg ccgccgctaa cgttaacggt gtagaacgcg agccaggggc
    29401 gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc agaatgaccg ccgcccagcg
    29461 tcggttaaat gcgcttactg acatgagttt gtccggtatc aatattgcgg ctaagtataa
    29521 ggagctacaa aaatagatta ttgatatgaa tcggtaatga tgcgactcat tactattcca
    29581 tctgtaataa agtacgcagc cctgccgctt tgttgtcgat ttcctgccac tcttgctcgg
    29641 ggtcggaacc acggacaatg cccgcgccag catataatcg cacgacattg ccgctaattt
    29701 ttgctgagcg cagggaaacg cagaattcgc tttgctgtaa tgagagatag cccgcagaac
    29761 cggcgtacca ttctcgggtg aacggttcgt gacgggcgat aaactgtcgc gccagatcgc
    29821 gcggtaagcc agcaactgct gccgttggct gcaactgatg taaacagatc acattatccg
    29881 ctttgttgag tgaagtccag atacagcggc gaagatgctg cactttacgc agacgcagta
    29941 cctgcggcgg taaaacatcc agcgtctggg tatcggcctg taaccgttgg cagatatctt
    30001 ccaccaccag catattctcg cgctggtttt tatcatccgc catcagccac tcacctaact
    30061 gctgcgcctg cttatcatca ggatgatttg ctaccgttcc cgccagcgct tcagtacgca
    30121 gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga gccaagaaaa gcattttcgc
    30181 catcaaaggc catgtaaaaa tggtagcaat tcagattcag tcgacgactg gcagccatca
    30241 tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt tgcccgagcg agcaccactt
    30301 tgtcgagctc accttcggcg atggttttcg ttgccagttc gattaattgc gtccagcccg
    30361 ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg taacccaggc aagggcttga
    30421 tactcaccag tgtggcgata aattctcttg ccttaatcgc atcgtgctga agggagcttt
    30481 cgctgaatag cgtcagccgc agcgtggctt taccgccaca gcgtcgccat tccaggcgtg
    30541 gtaaaagtaa attgccctgc gacgggtcaa acgcattcag cccccaaatg cgtaagtcgg
    30601 cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc caacgacgta aaacgggtaa
    30661 tcgcgcccag gacggcagct tcttcatcac cattacgttg ttgccagtag aattgcgggt
    30721 atgtttgctg actggccagc cagctcaagg catcaaaagc gtctttgaga gggaaaggaa
    30781 tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg cgacaaatgg cgcagtagat
    30841 tttccagcgc cgtagtaagt gattgcacgc ggacctctcc ctgttaaaag cctcacatta
    30901 tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg gaggttttaa cggcgtgcca
    30961 gcaacagtcc tagtaccagc ccaacggccg cgcccacacc aattccttgc cagggttttt
    31021 cgtggacgta gtcatcagca cgataaactg cctgcttcgc ccgatagtaa taactgtctg
    31081 aagcctggct aacccgtttt ttcacatcat ccagcgcttt ttctgcacgc gctttcagct
    31141 caacatattt ctgatcggcg ggatcgccag aggagcggag cacctcttcc agtgtttcac
    31201 taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa ctgattagac attctcgttc
    31261 tccatttgcg taaaacctgt cactaactat agacaagggt tgttgattac gcctgaatta
    31321 cctcgcgtgc catcccaatg tgtgggatac catcttcttc atagacctcc gtcacaggga
    31381 taaaaccgaa actctggtag aagttttgca gatgcgcctg cgccccgagg taaacaggct
    31441 tatcaggcca gtgatgcgtg caggtgtcga gggttttact catcagttgc tgccccactt
    31501 tttcgccacg caacgcttca ctgacaatca cccgaccaat aacgaccggc tcaagatcat
    31561 catcactttt cagaatcctc gcatatgcca ccagttcatc atttttccaa ccgagaatat
    31621 ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg ataagggcaa ttctgctcga
    31681 caacaaacac cgcgcaacgc agttgtaata aggcataaag ctgggaaacc gataattcag
    31741 agtgatgcag atcttgccat tcaatcataa tgctctcctt ttagatgtat gccttatact
    31801 agccggaata ttttttgaaa cgtgatgaac tcatggaatt aattttttta ggtacttcag
    31861 ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt gcttaacctg caacatccga
    31921 cccagagcgg actttggttg tttgactgcg gtgaaggcac ccagcatcag ctactgcata
    31981 ccgcctttaa ccctgggaaa ctggacaaga tttttatcag tcaccttcat ggcgatcatc
    32041 tctttggttt acccggcttg ctgtgcagtc gttctatgtc aggcattatc caacccttaa
    32101 cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac cgcgctgcgg attagcggct
    32161 catggaccga ttatccgctg gaaattgtcg aaattggcgc tggcgaaatt ctcgatgatg
    32221 gcctgcgcaa agtgaccgct tatccgatgg aacacccact ggaatgttat ggctatcgta
    32281 ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc attaaaagct gctggcgtgc
    32341 cgcctggccc actgtttcag gaattaaaag cgggcaaaac aatcacgctg gaagatggaa
    32401 ggcagattaa cggcgcagat tacttagctg ctccagtgcc aggtaaagcg ctcgctattt
    32461 tcggcgatac cggcccctgc gatgccgcac ttgacctggc taaaggtgtc gatgtcatgg
    32521 tgcacgaagc gacgctggat ataaccatgg aagccaaagc caatagtcgc ggccatagct
    32581 ctacacgcca ggctgcgaca ctagcccgtg aggctggagt cggcaagcta atcattaccc
    32641 acgtcagctc gcgctatgat gacaaaggtt gtcaacacct gttacgtgaa tgcaggtcaa
    32701 ttttcccggc gactgaactg gcgaatgatt tcaccgtgtt taacgtttaa ct
//
LOCUS       DAZPEP010000032        32654 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZPEP010000032 DAZPEP010000000
VERSION     DAZPEP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32654
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.032"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..347
                     /locus_tag="ACHUT9_001469"
     CDS             <1..347
                     /locus_tag="ACHUT9_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFR1347975.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
     gene            complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUT9_001470"
     CDS             complement(331..969)
                     /gene="ytfB"
                     /locus_tag="ACHUT9_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFR1347976.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUT9_001471"
     CDS             1188..1808
                     /gene="fklB"
                     /locus_tag="ACHUT9_001471"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1347977.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            complement(2290..2853)
                     /locus_tag="ACHUT9_001472"
     CDS             complement(2290..2853)
                     /locus_tag="ACHUT9_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347978.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPHQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            3018..3269
                     /locus_tag="ACHUT9_001473"
     CDS             3018..3269
                     /locus_tag="ACHUT9_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1347979.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKVS"
     gene            3271..5367
                     /locus_tag="ACHUT9_001474"
     CDS             3271..5367
                     /locus_tag="ACHUT9_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFR1347980.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRRVSEARAALVVEVLKLESAGNLGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VRQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRLAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
     gene            5443..6393
                     /locus_tag="ACHUT9_001475"
     CDS             5443..6393
                     /locus_tag="ACHUT9_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1347981.1"
                     /translation="MTDIDDVFNTINALINEGDKSQEDIADEAGMSPATLSALRKGEY
                     NGNVEGMHDKLQKWYQVWQKGQNLSGTPGVVKTQTFCDLHELFKTVRSLGIISVVVGV
                     PGVGKTFAAREYCRRTANAWMVTLAPAHSSVTECLLEIAYALEINNPGRNKGNITRAI
                     RRKLGGGKETSGRVNPLLIIDEADHLSVDGLEQLRAIQDATGVGMVLIGNPKQMADAT
                     RRGTDELARLFSRFAMTKQLRKVKKTDVMAVAKAWGVTQEDELDLLMRVAEKPGGLRV
                     LTHTLNHAWLAAKGAGTSLNKSHIKAAFKEAYSNPRLLNW"
     gene            6409..6636
                     /locus_tag="ACHUT9_001476"
     CDS             6409..6636
                     /locus_tag="ACHUT9_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347982.1"
                     /translation="MMSRNIKMATDAQNWLQAHGSHVNESYLGVARPVLEITCPPPEL
                     VRNAVRIMEHKSGVARSVWTARLNGCQIIWR"
     gene            6669..6932
                     /locus_tag="ACHUT9_001477"
     CDS             6669..6932
                     /locus_tag="ACHUT9_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347983.1"
                     /translation="MAKVIIEIKNTVSGIKGLNLRTSIAVDGSAELDGDEGTLAGMVA
                     LLVLNKSQKIINESAHEAIEILKNDSVITGGRVTEMAVEKTCH"
     gene            6947..7366
                     /locus_tag="ACHUT9_001478"
     CDS             6947..7366
                     /locus_tag="ACHUT9_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347984.1"
                     /translation="MNVKIRNEIRALIRIKERNNNGGDLREFICAREVEGYGEKTYLI
                     AFDHYSICARYCGEAVFRAITFGNAFNVDLWEYVMDREYICASDPKAREMWQRIWRDY
                     RLMAKGWARCCYSSLVLKAVQLSLRHIPASLREPLLY"
     gene            7379..7666
                     /locus_tag="ACHUT9_001479"
     CDS             7379..7666
                     /locus_tag="ACHUT9_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347985.1"
                     /translation="MTCNRKRWSREDREFIEASVGKMTVEEMAKKLKVAPTALQAHAR
                     RHGISLCAYRVSEHDKYLCRELYKEGLAIHVIAKKMELSNRAVSGIVYSEY"
     gene            7685..8209
                     /locus_tag="ACHUT9_001480"
     CDS             7685..8209
                     /locus_tag="ACHUT9_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1347986.1"
                     /translation="MAKPAKRIRNAAAAYVPQSRDAVVCDIRRIGDLQREAARLETEM
                     NDAIAEITEKYASQIAPLKTSIETLSKGVQGWCEANRDELTNGGKVKTANLVTGDVSW
                     RQRPPSVSIRGVDAVMETLERLGLQRFIRTKQEINKEAILLEPKAVAGVAGITVKSGI
                     EDFSIIPFEQEAGI"
     gene            8307..8855
                     /locus_tag="ACHUT9_001481"
     CDS             8307..8855
                     /locus_tag="ACHUT9_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFR1347987.1"
                     /translation="MKAYAYFKLSDAAANGYTIRWLKDVSVKRDKIICSLQRAFGADG
                     VSLVSNRGYERVECIWMNQLAPELWRGKSDGLFIGGFQFYGVVPDITTPEGRNNATLI
                     QEHEEQLRKYPTFPVWLCNELGVDVVPNMFDLKSVWTMHHSRDGFGILFEVCLLDGEV
                     KGPVPAECQRIKHSEFVALTEE"
     gene            8859..9398
                     /locus_tag="ACHUT9_001482"
     CDS             8859..9398
                     /locus_tag="ACHUT9_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347988.1"
                     /translation="MIDAKVLNGVSTLLRAYGRLTCGVLAEKMNMLPSSMVYFLRDAV
                     DAGVLTECNGFYDVPRPRLTSPVRRNTTELPGVDDAAWCTFRRSLPWLEGNAIPALAK
                     EFATGVLTCESVYIVAEVDDEMCKQGMPRFVMAYIDIRLGRFICGSSGWNITDHVLRY
                     LILDYSPVPAETQEISGHD"
     gene            9391..9573
                     /locus_tag="ACHUT9_001483"
     CDS             9391..9573
                     /locus_tag="ACHUT9_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347989.1"
                     /translation="MIKSIGFVLLVGTCGNDACDAIPVTEKIWPTEQACMQVMERIQK
                     RYPGEIFYCEDALRNE"
     gene            9566..9928
                     /locus_tag="ACHUT9_001484"
     CDS             9566..9928
                     /locus_tag="ACHUT9_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347990.1"
                     /translation="MSKRIYDDPKYRAEMRAQLVVSGYTPEQADATLTELMGERPFDE
                     AEYEYWLKQFRLEILENTTLPSLIRIIKSVIDNACYNGDCLAAAIATHALVNLLEVTG
                     AVFLNPEKVLRDENTDDY"
     gene            9906..10241
                     /locus_tag="ACHUT9_001485"
     CDS             9906..10241
                     /locus_tag="ACHUT9_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347991.1"
                     /translation="MKIQMITDADLMAATLISSGMSEQKVNDFLDNVVYQRYEQAREN
                     LISEKRTMIAESLSPAWIIEELRKIIASQDKVAAVAALDVLHRMSKELFNTELILNPG
                     GHNEPHIPD"
     gene            10219..10770
                     /locus_tag="ACHUT9_001486"
     CDS             10219..10770
                     /locus_tag="ACHUT9_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFR1347992.1"
                     /translation="MSRTSLIKLIHVARRDLQLDDDTYRTFLVQCTGKTSCRELSVAQ
                     LERVLDAMKERGFKKQKKHPRRRFKGHVTPREKIYKIWQQMFLDGFVSDISDAALDKY
                     VERLTTRRNGGQGVSTLAWCHGESLQVVLETLKQWHMRCIREAFARHGVPLPVSKTGQ
                     ELRGYDALTSAYARARNSGRIAV"
     gene            10767..11216
                     /locus_tag="ACHUT9_001487"
     CDS             10767..11216
                     /locus_tag="ACHUT9_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000812146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFR1347993.1"
                     /translation="MKQMKEQDLFEDFRDDSVLEYLNDYQENTAYPALLSELNALLRK
                     ELARIGADPAHSLELVVAICRHIGGMQVYVPRGNILENLVRDMRIWRDFNGHNIPELV
                     QRYGVTYKTVYKAIKRMRRLECNKYQPDLFYHSPNLPHPVRRPPRRP"
     gene            11302..11889
                     /locus_tag="ACHUT9_001488"
     CDS             11302..11889
                     /locus_tag="ACHUT9_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFR1347994.1"
                     /translation="MENLKFSPAFEHALNFILRPDIEGVYVNDPTDRGGETRYGISDR
                     RDGVIDGKTDVSGDGKPDTRIRDLTREQVAQIYWRDYWLPAGCDQWPDGVAMFVFDAA
                     VQHGVKKAIRILQEAADVDADGIIGPRTRKAVSLSTPDWLLTRCIVRRSRFYADIIKS
                     KPAQGKYLNGWFNRMEKVTDACLEIIDNVSSVTRG"
     gene            11891..12118
                     /locus_tag="ACHUT9_001489"
     CDS             11891..12118
                     /locus_tag="ACHUT9_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347995.1"
                     /translation="MGKGWDASLRAGRRDRLRQEVLHRMAGGPPPKPLDYTGHDGTHA
                     SHYMRGWNTVDTRDIFWQCQKYKEKLNVQNE"
     gene            12102..12512
                     /locus_tag="ACHUT9_001490"
     CDS             12102..12512
                     /locus_tag="ACHUT9_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005110321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347996.1"
                     /translation="MYRMNSLFVRVIYSRFMLCQWSFLGLSAVLFLASCFAGWQAFAV
                     MVLAYGATLCLIHSFQLGNVPYRLQPKRRFRAASLRIVVWSWGVWAVGFFMLTFGLLY
                     LKEPYQLAFWLGGIFCAALYKHQRRFHKGDSWTR"
     gene            12500..13108
                     /locus_tag="ACHUT9_001491"
     CDS             12500..13108
                     /locus_tag="ACHUT9_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1347997.1"
                     /translation="MDPLTLSGIASVLLKAGPGLIRSVGRWFGGSTSAAADSVAGMVE
                     SVRESLPVAEQQRILEQKLVMLSPEQLVQLETLKVQLQQLEVERQKLVLADQQAAHHE
                     QQETIRNGDNATDSYVRQTRPLLARLSCYSSLAYVLLLSCGQIAGAVAGANGITLHMP
                     SPDWDITLMLFTPALGYLGVRTLDGFARYSKSSCHKISAGPK"
     gene            13105..13332
                     /locus_tag="ACHUT9_001492"
     CDS             13105..13332
                     /locus_tag="ACHUT9_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000128965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HFR1347998.1"
                     /translation="MTDETDRISEVVLNERQDVVNAWLMRIKEAPHSRGFCNDCGNVI
                     PAQRIAVLPDVVTCIDCQQARERRRKTCPGK"
     gene            13317..13622
                     /locus_tag="ACHUT9_001493"
     CDS             13317..13622
                     /locus_tag="ACHUT9_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFR1347999.1"
                     /translation="MSWEVIRANWPVLWALLMTAINLIQLILAKTYVKREEFDSLRSR
                     VSVMEGLVNQLPDRNEFHRLQLDISNLRGEIKELGPSIRQVSRISDLLLENELKEKN"
     gene            13628..13915
                     /locus_tag="ACHUT9_001494"
     CDS             13628..13915
                     /locus_tag="ACHUT9_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005103431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFR1348000.1"
                     /translation="MKEILTEDRRLVLLRSLLDCGDSANESVLQTCLQAYGHKVSRDV
                     VRTQLAWLREQGLVRLSDVGGCYVAEITGSGEDVANGLSGVPGVKKPRARD"
     gene            13918..14205
                     /locus_tag="ACHUT9_001495"
     CDS             13918..14205
                     /locus_tag="ACHUT9_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348001.1"
                     /translation="MARKLKPLSRGERAVVRQLAYCLVLADIEQNAIVRAYEKHTGKP
                     WNPDAPDTPVKRALRSSPAYARLWKLPGKDIQSVREEIYAGLKTQRTEDGK"
     gene            14195..14776
                     /locus_tag="ACHUT9_001496"
     CDS             14195..14776
                     /locus_tag="ACHUT9_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3486 family protein"
                     /protein_id="HFR1348002.1"
                     /translation="MENEQRPTRGRLSKVDLLPDSIREQLHQMLREKRHTQEEIREAI
                     NALIDEHNLPGEMQLSRTGLNRYASRMEKVGAKIRASREMAEVWAAKLGSAPTSDVGR
                     LLMEFVKTLAFETSMSMTEDDKPVAPKALGQLALVAQRLEAAAMTSHKREKAIRDAFA
                     QEMAEKTEELVRAGGLSGGAADTIKREILGISV"
     gene            14773..16308
                     /locus_tag="ACHUT9_001497"
     CDS             14773..16308
                     /locus_tag="ACHUT9_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1348003.1"
                     /translation="MTQMNAFSESDVLLPYQKRWVADDSKLKIAEKSRRTGLTWAEAA
                     DAALTASLKKENGGRDHFYIGSNKEMAREFIDAVAMWAKAFNAAAEEIREEVITDEDK
                     DILTFVVYFASGFKVKALSSNPGNIRGMQGNVTIDEAAFHEKLDEVLKAVTPLTTWGG
                     KVRLISTHDGVDNPFNQLIQESRAGKKDYRIHTITLDDACNDGLYRRICQVRGMVWSP
                     EAEVEWKEGLLRNTATREDALEEYYCVPKNGGGTYIPRSLRERAARGTGKVLRFTGTP
                     EFNALTEGQRRAEIREWLETVVRPELEKLPKNLRHCLGEDFARSGDLTVLAPVTVNDD
                     TTREVPFLVELANVPFKQQEQVLFWLCDRLPRRDGIKMDARGNGQYLAEQAAERYGDE
                     VEQVMLSVAFYRENMPRFRAAFEDDELILPKHEDVINDLGAIQLLRGVPGIDDARTKG
                     SDGRKRHGDAAVAIFLGFLASKDDCHRYELHRLNRPAKPEEGNARRQMKLTRGLKNEG
                     GLL"
     gene            16305..17876
                     /locus_tag="ACHUT9_001498"
     CDS             16305..17876
                     /locus_tag="ACHUT9_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFR1348004.1"
                     /translation="MNLKQLAGAVRRLLNPATGEEDTLKKEALNEAQARPRHAGVRPT
                     SPGISVASGLNPGRLAGILRNAADGITRDFFILAEEMEERDLHYASVLRTRKLTVAGI
                     EPVVVAASDDDADVQLADAIRALMEAPQIPELMFDLLDGLGKGVAVCEILWDTRNNHW
                     MPRDYEWVDPRFLKAEKPTLRQFRLLTDDEPVDGVPLTPGKFIVHQPRLKSGLPLRNG
                     LARLVAVMYMLKSFTVRDWWAFAEKFGIPVTVGKYGPNATEEQIRVLIDAIASIASDA
                     GCAIPQSMQLEMQETASRNNGGALFREMAEWCDAQISKAVLGQTMTTDNGSSRSQADV
                     HNQVRMDIVRWDARQLANTLNEYLVRPYIEANYGPQAHYPRVVLRISEAEDLKALTDA
                     LVPLIDRGMRVQESELRDRFGLAEPDEGADVLHPVSAGMPGDMAMNRERVALNREHPD
                     ELAQMVDDALRDWQKTGEAFTNPVLTLAQECDSFDDFLKRLPELQETLNADDFALQLA
                     EVCFKARALGDTAHA"
     gene            17869..18633
                     /locus_tag="ACHUT9_001499"
     CDS             17869..18633
                     /locus_tag="ACHUT9_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1348005.1"
                     /translation="MRETLIPKEALAWLKAKKLRPGFDYRDVWREEHRNSFTVAKMLQ
                     LDLLSDVKALVEDALQSGQTFSEFREMLQPLLIKRGWWGVQEMDDPLTGETRTVQLGS
                     DRRLRTIFDTNMRTARAAGQWERIQRTKQAMPYLMYELGPSREHRVEHVKWARLCLPV
                     DHPFWQTHFAPNGWGCKCTIRQVSRGEYAQLAAQGTIHTEAPEIRTVRWVNKRTGEEE
                     DVPEGIDPGWNYNPGINREQELARQLAARQARFNSE"
     gene            18848..19804
                     /locus_tag="ACHUT9_001500"
     CDS             18848..19804
                     /locus_tag="ACHUT9_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFR1348006.1"
                     /translation="MQPELLALCFSLPEPIPELTPAQLPEWLELVPAGEFTGRDGRTW
                     MNRNPHEVVARSSDINIPVDIEHATEIKGPRGEEAPAYGWVEALRVTDSGTIEGRVVW
                     SESARWMLSERRYRYYSPAFFHDADGAVTRLSSVGLTNKPNLDFPALNTEKNPMTVPV
                     QITGLLGLAESATVDDTVAAIKQLQENEQVALNRAQTPDLTKFVPVETHNLALNRAET
                     AEKRLQQLEEKEAEALVDAAIGAGKVAPANRDMFLATCRTEEGRKQFAEYTKGAQPLV
                     NNDKPSQGKDKPAQTLTDAELAMCRSMGITGEEFLAAKPKQE"
     gene            19812..20717
                     /locus_tag="ACHUT9_001501"
     CDS             19812..20717
                     /locus_tag="ACHUT9_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFR1348007.1"
                     /translation="MSQTISSEILHALTTCLSAAFTRGLSGVEPQWNRIASEVPSSSA
                     SNTYGWMKDLPDIKEWVGERQLATLDGYGYTITNKLWESSIRVKREHIEDDQIGQYSI
                     TAERYGRMTAVFPDKLCYALLCAGFNTLCFDGQNFFDEDHPLGDGTYSNVVGTPASDK
                     GEPWFLIDESQVLKPIIWQTRRAFKFEALDDLNSEHTFKNHEFLYGVDGRCNAGFGFW
                     QTAVGSHAALTVENYKKANELLRGMKGTNGEPLGIRPTTLVVGPKNRADAKLIIDAML
                     VNGGDSNIWYKDVKIVDSPYITTPA"
     gene            20789..21100
                     /locus_tag="ACHUT9_001502"
     CDS             20789..21100
                     /locus_tag="ACHUT9_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348008.1"
                     /translation="MSEKAETKGAKAAKNSAAQENPAPLADVIVADGQANEPRPAEDP
                     VAVQGDAPVRLNVRAVSENGFWRCGRFWSHIGEDVAVSAAVATRLMAEPNLIVREAEK
                     G"
     gene            21100..21537
                     /locus_tag="ACHUT9_001503"
     CDS             21100..21537
                     /locus_tag="ACHUT9_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016807456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 domain-containing protein"
                     /protein_id="HFR1348009.1"
                     /translation="MGYITQEDLLRADGNLVWNMAINRETNGLDEDKIRQAISDAEAE
                     IDSFLSRRYQLPLGVTEIPRPLQRVAVSLAFYWLSERDNQITELIQKRYDDAIKTLRE
                     MANGTRDLGLPTYATPAETDNGKIIVVGANARLFTRNNLKGVL"
     gene            21537..22079
                     /locus_tag="ACHUT9_001504"
     CDS             21537..22079
                     /locus_tag="ACHUT9_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004114586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1348010.1"
                     /translation="MGISVQVSGDQRLEDIRRAVEKLAEGSLQSELLESIGAVVESQT
                     RRRIIDEKTSPGGERWPDWSDGYKKTRHGNQSLLRGEGHLLESIQYIVENRVVRIGSP
                     LDYARIMNDGFSGSVPVSAHKRLISQCFGRALKYPVWQTVGAHHRMMNIPQREFLGLS
                     SASQQELQQVISHFWKEVLP"
     gene            22076..22687
                     /locus_tag="ACHUT9_001505"
     CDS             22076..22687
                     /locus_tag="ACHUT9_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348011.1"
                     /translation="MTEQRPELRTPGSTVAAAERIVAWLQTALQGDTPDRADVVERHI
                     GQFNSTDEVKRYLSGRTGCIRVAALRVRDINPRGGLSGLVTWVAYIMATDSWGYSRDV
                     RCEVLAGKVIKRLLSSDATGGMGAERMAADVRADNIYSVSLDGLGVTMWAVTWEQEFR
                     LDEEIDLAALPDFLRLGATLRSGEHTEINDVIHVRGDDGTETD"
     gene            22668..22853
                     /locus_tag="ACHUT9_001506"
     CDS             22668..22853
                     /locus_tag="ACHUT9_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFR1348012.1"
                     /translation="MEQKLIKPARENVRVRKPDGAHLSPEGERLNVCAYWLRREAEGD
                     VEITTLPKNNNKTRGKK"
     gene            22853..24328
                     /locus_tag="ACHUT9_001507"
     CDS             22853..24328
                     /locus_tag="ACHUT9_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1348013.1"
                     /translation="MSLGSIPDDIRVPLVWIDIDNSQALEGASAQSRKILVMGHAVSS
                     GSADALSLTRITSDSQADQLYGKGSMLAEMLKMLRRANTYTETWAMPVAAPEGAAAKA
                     TLTVLGTATEAGTVALLINGVSVQVSVSAGATKENIAKAIADAVTKKPATQVAAAVKD
                     DATDTVELTVNWHGVTGNGADVRLNYHTGEAFPAGVKVTATAFTGGTGTPEMADAVAA
                     IGPEWFTDIIAPFTDTKSLNTLRDELLNRWGPLKMMEAQLWTAFRGTHGETGTFGETR
                     NDWLISCIGTNLSPHPAWMWAASYGATAAYHLAIDPARPLQTLVLTGILPPARNVRWD
                     MPERNLLLHDGIATHMVDAGDNVCIEREITMYRVNQYGDADVSYLDVQSPATLGRIRY
                     IIKNRFTNRYPRHKLADDDVLDSLDAGQPVMTPKLCTAELLDICQTELLPAGLVENFS
                     DYKDTLQVTRDSSDKNRLNFICHPNLVNQLRVLAGLIQFKL"
     gene            24338..24694
                     /locus_tag="ACHUT9_001508"
     CDS             24338..24694
                     /locus_tag="ACHUT9_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1348014.1"
                     /translation="MAKILGMATIRVNGREIKTEGKSTLNPGGFSRTQHMGGGKVWGI
                     SSKMASPSIKVTIAAAADMDVIEISSWEDVTVMFYGNNGLNYMMTGSATDNPAELDED
                     SGTISANFIGEKCVKV"
     gene            24696..25091
                     /locus_tag="ACHUT9_001509"
     CDS             24696..25091
                     /locus_tag="ACHUT9_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1348015.1"
                     /translation="MAAMEFELKHGLLTGKGTADETLHKTVKLRELTASDVIDAQLAA
                     ERVVMGGNGKAVAYCSEVLMGLEMMRRQVASIGNIPGPLDMKQLRMLHPADLELISTK
                     AAALDEMLEEVATRGRTDAAGGGSDEPAG"
     gene            25178..26971
                     /locus_tag="ACHUT9_001510"
     CDS             25178..26971
                     /locus_tag="ACHUT9_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1348016.1"
                     /translation="MATGNRLSTEIMINLAGNLTAKARQYGANMSQFARNNQKAMRLV
                     KATTEAAGRGLDTLGNRYTAMIAGLGSSLTIKQVADFDAQMRRMGTDAQLTTEQVKTL
                     HDKIRDVSNQKDIRIDASALGQGASELLGKTGDYQYVVDNLRNMGLMMQAFGVDGQVA
                     AGLMAQFWEKNVRGADAVNNMMDRLYSQFAVGSVSVADVARAAPKLFSIIQDQGPEAI
                     AQMGAFAQVFAKNKGSIDETVTSIQAMYASLSDKKNIEFLKKNGIDVFVKGTKDIKKP
                     YELMMEILKRAKYDPLKLQDVFDLTGMQGIKALLNPENRQLMEQMIYGTIELGSTQKA
                     AQTNAEGFNAAIQSLNNEWQRFAEGQLAKPVQDLADALNSVDQNTAQNWLQVGKYMAI
                     ALGGIIAIRKTYQFGKTIHDIMNPKGKGKGIPGSITDVFGSGVMPVYVVNMGSGGMKG
                     NTGGLPDAPDSSRNPRNSRNPRGPKTPALVTPQTASGFNLLDMVLSAFPKNKEEADAL
                     IARVQENNNRPTVWTDIRNWFNSLEKKNIADPRPWAGMQPTQNYPFLPQQLQGEIRVV
                     VEGDARVKSVRVDQPGVRLSAQAGVTSVEQG"
     gene            26971..28245
                     /locus_tag="ACHUT9_001511"
     CDS             26971..28245
                     /locus_tag="ACHUT9_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR1348017.1"
                     /translation="MTTSNGKWDGLRDASFRGVPFFLVDTEGTGGRRAIPRAYPRRET
                     AWTDDNGAVPGQQQINAKLPGKNFRDDLNALLDALNTPGPGELVHPWFGIQTVQVGKV
                     THRLSTEEDGIAYVTFEVFEAGERLFPSAADNTQQEVLTGIDAVKAAIDDGDWFGALD
                     GLGDMADSFLADMENLVANLPTLPAALNQWMDRLKHFKAMAGTLIATPGRLVSELSAR
                     VEDIAGLVTEPPEALAVYTTLRNQWAGERARQIATGALPEDITVKPGSVADGNPGFAI
                     GLSPDYQPVSDSLQKNIDDFRQVVVLGALLGQASAVASMTFDTRDAALSAGDTLAAEL
                     HEQAVAAVENNQRTLWRTLRDLRQAVITDARERAARLPETRQVTLTTTTSAALLAWRE
                     HGDTRRRDEIVQRNRLRHPSFILPTQPVEITD"
     gene            28245..29315
                     /locus_tag="ACHUT9_001512"
     CDS             28245..29315
                     /locus_tag="ACHUT9_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR1348018.1"
                     /translation="MESVILTVDGKLWEGWTEMSVSRSLKAIAGEFELSVTTRWSAAA
                     PRVIREGQPCTVRLGADTVLTGYIDDFIPGYDAENVEIRVMGRDKTGDLVDCSVVHSS
                     GKWKGVRLEQVAADVCRPFGITVITETPTGEAFASVVLEQGETGFELLDRLAKQRGVL
                     LTSDGAGNLIITRASSVRAGVSLVLGKNILAARGRFSWRERNSQYIIKGTTSAGGKLW
                     DSQPAPMVGGRQYITEDPEINRYRPRILVNEDSLTVGGASIRGEWYKARMLGESRTTE
                     ITVAGWREQGDSGPLWQTNRLVDIDDSIQNLKTTWLISGVTWTDGAQGRMTVLALVPP
                     ESLDMPEMKAKTKKTKAVATWD"
     gene            29306..29842
                     /locus_tag="ACHUT9_001513"
     CDS             29306..29842
                     /locus_tag="ACHUT9_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1348019.1"
                     /translation="MGLNAIARRLRLMVDRAVVRLVSDGLARQNLQIQTLADATDDDV
                     ERFQNYGFTSVPPEGSEAIVLAVGGRRAGLVAIAVEDKRCRPKGLSPGDVRVYHRDGK
                     SHITLKENGVIEITGEQVDVSGKTVNLTADELLNIIGKQMKFVGPCEFTEDVRIKGKS
                     FSEHIHKDGDNEKTSPPV"
     gene            29839..30291
                     /locus_tag="ACHUT9_001514"
     CDS             29839..30291
                     /locus_tag="ACHUT9_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR1348020.1"
                     /translation="MTTEIRWNNMLSRGDITVTHNGLSRDEGLVTQVLICLFTDARAD
                     DDDVIPDGSGDPRGWPGDTYSDFSWGSRLWLLEREKLTEDVRLRVEDYARLSMQPLLR
                     AGYARNATVTASIIIPDRIAFLVVLTRPDKTTLRIEITRRWEATINAV"
     gene            30281..31357
                     /locus_tag="ACHUT9_001515"
     CDS             30281..31357
                     /locus_tag="ACHUT9_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1348021.1"
                     /translation="MPYEIPTLGKLIADGEKDIAYELGLQKLPPVSVEQALNVSFSSQ
                     VRDLYDHQSWIKDQIIPSTTSDDETIIKTAKYEGVIRKQATFASGRVTFTSQSPLPAE
                     TRMQSDTNQVYQVLTSGEVQDGEVTVIVQAEEAGVAGNLAAGAVLTLLSPLPGTASTG
                     AVAEGGITGGADTESIAALLDRLLYVRRNPPTGGALHDYVIWAREVPGVSRAWAWDAW
                     HGPGTVGLAWLYDDRENIVPTREDLKTMEQYLFCHKHPATGVMVGKPGGIEVWPVQVR
                     LKKVSISIRLTPDSLANRSAVRANLTALQKTLAPGQTLPVSSLRTAIGMTSGITDYFL
                     NIGEDTTSDVDELITIGEVTWLTA"
     gene            31342..31926
                     /locus_tag="ACHUT9_001516"
     CDS             31342..31926
                     /locus_tag="ACHUT9_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR1348022.1"
                     /translation="MAHSVTEWLAALQQVMPRGKAWPRDNDADLNRFLRALAERLNRT
                     EYDASRLHVEMRPETTLRLLPEWEQYLALPECGIAPTTREARRRAVVEKYRRRGGLAT
                     WQTEAAAAALGFTIKVTAVLPHHCLRDCMYPLHPARYRWMLKVNVPGKDAGRFTCIDG
                     VMTPLISERTRELECLLKHYRLAGTEYEYYYTGE"
     gene            31930..>32654
                     /locus_tag="ACHUT9_001517"
     CDS             31930..>32654
                     /locus_tag="ACHUT9_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1348023.1"
                     /translation="MFYVDNPTGVPVMPPVAAELSKTTLYFTEGGNGIPPTYPGPDWF
                     NIIQSELLEILRQANIKPDKNTTDQIMTALKKLFITNSGSAGAIAGLTGQNNTFPYFT
                     GKDTMALTPLSAFVRGILGKESATDFADALKVIKQSGGTMTGELKIRGVNALRIFNEA
                     FGLIFRRSEECLHLIPTSEGQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNG
                     ALGIGVQNALGGNSIVLGDNDTG"
BASE COUNT         7899 a         8312 c         9494 g         6949 t
ORIGIN      
        1 acccttggtt tttaaaaatt gtgcatagtg aaaatcaaag taaaggtgtg cattatgcaa
       61 aatcactgcg tctcttagaa attaaccatg cgcacttgca gttaatggaa tccttattgg
      121 atgaagggaa gaaacacaat atttttaagc cagatatcga cccattgcag gtgaatatta
      181 atattgctgc gcttggggga tattatttga tcaaccagca tacgcttggc ctggtttatc
      241 acatcagtat ggtttcaccc caggcactgg aagccagacg taaggtcatc aaagagacaa
      301 tccttagctg gcttttggtt gacccttcat ctaccgcgca cgaataaaac tgccatccgg
      361 ctggcgggta aacaggacct gttgattatt ccccgtatca atggttaagc ccgtcaccac
      421 gccgctggcg ttctggcgga ttttcaccat ctgcccattt tgcaaattac tcagcggctt
      481 acccgcgcct tctacctgcg ccattgcata gacatcggta gcgggcagtc cgtggtcgcg
      541 gaacagctgc gccatcgttt taccgggttc gacacggtaa gagcgccatt gattatcaat
      601 tccgctatct ggctggaatg gttgcgtttg ggtagtttgc ggctgttctt ctggctggcc
      661 ttcctgtata ggttctggag cgaccggagc cacctgatca ggatcatttt gtggtgtgac
      721 taattgcgcc cgcagctgtt cttctgtcgg cggttgtgat tgcgactgaa tatctaactg
      781 cgcttcacgc gtcaccaccg gcgcgttggg cgtatcatca gatggaagca gaaatccgac
      841 caccaataca atggcggcaa taatgatccc ccgacgatgc atcggcggca gcgggtccat
      901 gaagcgaaaa ttgtccggcg cgtgccagac tttctccagg gttggtttta gttcaaagcg
      961 cccgggcatg gtttcctcct gctccgcgtc ttgttcctca atcatagcct atgaataagc
     1021 taacgctatg atgtccgtgg taaacccgcc tttattatat tcatacggga tattgctatt
     1081 gtttcttttt ccccgggatt tgtcatcatt cctgcgacaa agttttaccc aaagaagtgt
     1141 ggctgatatg ctgcccgcta cttttatacc ctaagaaagg aaatacgatg accaccccaa
     1201 cttttgacac catcgaagcg caagcaagct acggcattgg tttgcaggta gggcaacaac
     1261 tgagtgaatc tggcctggaa gggctgctgc cagaagcact ggttgcaggt attgccgatg
     1321 cgctggaagg caaacatccg gctgtgccgg ttgatgtggt gcatcgcgcg ctgcgtgaaa
     1381 tccacgaacg cgccgatgcc gttcgtcgtc agcgtttcca ggcgatggct gctgaaggtg
     1441 tgaaatacct ggaagaaaac gccaaaaaag aaggtgtgaa tagcaccgaa tctggcctgc
     1501 aattccgcgt gatcaaccag ggtgaaggcg caattccggc acgtaccgac cgcgttcgtg
     1561 ttcattacac cggtaaactg atcgacggca ccgtgtttga cagctccgtt gctcgtggtg
     1621 aacccgctga attcccggtt aatggcgtga tccctggctg gattgaagca ctgactctga
     1681 tgccggtagg ttctaaatgg gaactgacta tcccgcagga actggcatat ggcgagcgcg
     1741 gcgcaggcgc atccatccct ccgttcagca ccctggtgtt tgaagtcgaa ctgctggaaa
     1801 tcctctaagc agcgcattct gttcccctcg aaccagaggg gagcaggcat tcagcaataa
     1861 acccttcagt ttgccaaacg gcgctatttt gtgttgcaaa gaccccgtaa gcgtgtattt
     1921 ttgtgagctg tttcgcgtta tcaccgtgat atgacactca ctttaaacat aaaattaaca
     1981 ttagatctaa atcttagtat tcatcccgcg tattgtatga ctaggtaatt tattcaagat
     2041 aaccgggaag agattaaaaa agacgggaat agattggaat caacaagtta ggaactcaag
     2101 gttttcaaga taaagcgagt ccaaaaatgt taaacggttc gatatcaata aaacattcaa
     2161 gataactcac aggttcttac aatagccttt aaagaccgtt caaaaacgat tcaggtgttt
     2221 attatagcgc tgatgggtgc aagcgccaaa accaatgtaa ttttagcaca gctaggcact
     2281 tgcactgggt tatgcgctgg ctgctttctg aggtagattt aagtgatcgc ccttagcttc
     2341 ggacaacgcg taaatctctc taaccttttc gtctggcaaa ttaccaaggc ttaacgctat
     2401 ggctctacga actccaacta gagcgtgcaa ctcttggatg tgctcatcca gaagaatgag
     2461 taattgctcc gctccttttc tcccaagaac atcagctact tgttgttttt cttgtggttt
     2521 aagaaatgac aagaacatta gaatttgagc atcaacatta tccgcattct cttttttggg
     2581 ttctttggaa tcttttagtt caacatcacc ctctcccgtc atgagccatt ccaaggtaac
     2641 attttcgctt tctgctatct tttttaactg atgcgggcgt ggtatcggtg cgtaatcccg
     2701 cctgttgtaa taatttttta aagtgccttc attgatcccc catgcccgag cagccgcatt
     2761 tcttgaaggg tatcgttcta ctaacctaaa gattctttcg ataatcggtt cttttccatc
     2821 ggcaggaaaa gaaccttggg gtgcttttct cattcttggt tcctttctat ctaattcatt
     2881 gataaataat gaaattaaaa aataaaccat aaaaacacca aagaaccctt tacaaggaat
     2941 ctttggtatc ttaatcttta tccatacgga tatccgaccg gatatccgcg cgggtaattt
     3001 tgtaggttaa aggaaagatg agacaaagag aagatagtga ccgcgattgg ccttcgctga
     3061 agattcagct ggcgctcaaa gataacgggt ggtcttcctt ccgggagctt tcactccatg
     3121 ctggttatgc tcaggattca ctgaaagcgg tagttcgcac accaaacaaa aaatacgaac
     3181 gcgtaatttc tgacgctata ggtgtaccgc cagaaatcat ctggcccagc cggtatcgta
     3241 acgatggatt tatcaggaag gtgtcgtgat atgcagtcgt acgttaccgt aaatgacctg
     3301 ctcggcgtac caggaatgcc agccaccaca aaaggcatcc gacaggcgtt acagcgtttt
     3361 tctcgtgatc ttggtgacgt tagtcgccgt cgggaaggca caaaagccat tgagtaccac
     3421 attgattgtc tgcctgaaat aacacgaaag gcactgcgtg aacgctatgt ggaacagctg
     3481 gtggctacag agaacaatgt ttctgaagtg aaagctgtta ccagaaaaac acgcaatcct
     3541 gacgctgtcc aggcaatcga agcatatcgc ggttctccac aactgatgga agaacgcctg
     3601 aatgcgctga ccgaaaacca gcgccgggta tctgaagcaa gagctgcgct ggtggtggaa
     3661 gtgctgaagc tggaaagcgc cggtaacctc gggcgactga aagccattaa ctttcttgtt
     3721 gaaaaagccc gtaaaggtga gctgccggag cgcctgcaac aggccgcagt taacgccaat
     3781 gcaaaacgtg gcgctaatcg tactatcagc cgcgatccac tttaccagtg ggttctgaaa
     3841 tacaaccagt cgcagaacgc cgccgaacgg ctgctgttac tggcaccggg aaaacgcgac
     3901 gaaataaaac cggaagaaat cagctggctg cctgaatttc tggcgcagta tcggcaggtg
     3961 aacggcagac ccatgtcaga agcctacgag gattttgtcg cagaatggca gcgtcgccat
     4021 gcggacgaac cgtacatgct ggaggtgatg ccttcctatg atgttgtccg gtatgccatg
     4081 aaaaaactgc cggaagtggt gagacaaaaa gggcgcgtta ccggcagtga ataccgtcag
     4141 cttgaaggat tcacccgccg cgactggaca gctatgccgg tgaattatgt ctggattggt
     4201 gacggtcacg gtatgaagct gaaatgtgcg catccgatcc acgggcgtcc attctcaccg
     4261 gaagtcacgt ttgtgatcga tggtggaaca cggtttgttg ttggctggag tcttgacctt
     4321 gccgaaaacg tgttcgccgt tgccggtgcg atacagcacg gcattcgcaa ccacggaaaa
     4381 ccgttcctgt attactcgga taacggttcc ggtgaaacgg ctgacatgct ggacaaagag
     4441 gtcgtgggta ttctgccacg cctggggatt aaacacccta ccggtattgc cggtaatcca
     4501 cagggacgcg gcattattga acgcctgaac cggacactgc cgatgcgtat agccaggagg
     4561 tatcgcacct attttggcaa gggtgctgac cgggaatcgt tgcgtgtact taaccgtgat
     4621 ctgcgttcag cgtttaacgc cctgcaacag gacaagcctc tgaacgaccg ccagaaagcg
     4681 gcaatgcgcg agctgccatc gtgggcggag cttattgagg ctattcgtga aggcgtggaa
     4741 tggtacaaca accgcccgca ttctgagctg ccgatgaaac cgaacggacg gcattacagc
     4801 ccgacagagt tcagaaaaaa acgcctggca gaagaagaca ccgaaattga gtggctgagt
     4861 gatcttgagc tgcgcgacat gttccgccca atggtggaac gaccggtaag acgctgcgaa
     4921 attcagtggc tgaacaatat ttactacgcg ccggagttgc gtgacgaaca cggtcgcaaa
     4981 gtgcttatca gttatgacat tcatgacgcc gagcgaatta cggtacgtcg caaggacggc
     5041 agctttatct gtgaggcgat atggaacggc aataaacgcg cagcctttgc tgtcagtgct
     5101 gaatatcaca aacaacagca gcgtattaaa ggaatgcgca aacgcgctga ggaaaaaatt
     5161 cgtgatgctg aagatgaagg tattcagatt ctggaacaca aacaggctga accctggctg
     5221 agtaatgttt atcgcccggt tggtaatgtg gtggcagttc agcaaccgga atatgaagaa
     5281 gagcatgacg aagaatttga acgcgatttc cggctgggga tgcaaaaact ttttgccatg
     5341 caggaagaag atgatccgct ggcctgaata aaaaaacagt ccgcgggaac ggactgttaa
     5401 ataaaggaaa caaagctgat ttgaaataac cgaggaaata ctatgactga tattgatgat
     5461 gttttcaaca ctattaacgc tttgattaac gagggcgaca agtctcagga agatattgct
     5521 gatgaagccg gaatgtctcc ggcaacactc tcggctctgc gtaaaggaga gtacaacggt
     5581 aatgtcgaag gtatgcatga caaattacag aaatggtatc aggtctggca gaagggacag
     5641 aacttgtcag gtacccctgg tgttgtgaag acccagacat tttgtgattt gcatgagctg
     5701 tttaaaacag tgcgctcact cgggattatc agtgtcgttg ttggcgtacc gggtgtcgga
     5761 aaaacctttg ctgcccggga atattgtcgc aggacagcta atgcctggat ggtgactctt
     5821 gctccggcac attcgagcgt aacggaatgc ctccttgaaa tagcatatgc gctggaaatt
     5881 aataatccgg gaagaaataa ggggaatata acccgcgcca ttcgcagaaa actgggtggt
     5941 ggtaaagaaa ccagtggccg tgttaacccc cttctgatta ttgatgaagc tgatcactta
     6001 agtgtggatg gtctggagca attacgcgcc attcaggatg ccactggcgt ggggatggtt
     6061 ttaattggca acccgaaaca gatggcggat gccacccgtc gtgggactga tgaactggcc
     6121 cgtctgttca gccgttttgc catgacaaaa caactgcgca aagtaaaaaa gaccgatgtg
     6181 atggctgtag ccaaagcatg gggagtaaca caggaagatg agctggacct gctgatgagg
     6241 gtagccgaaa agcccggggg attgcgtgta ttaactcaca cactgaatca tgcgtggctg
     6301 gcggcaaaag gtgcaggcac atccctgaat aaaagtcaca ttaaagcggc ttttaaggag
     6361 gcttacagca accccaggtt actgaactgg tagtaaagga cataaaccat gatgtcacga
     6421 aatataaaaa tggcaacgga tgcgcagaac tggttacagg cgcacgggag tcacgtaaat
     6481 gaatcatatc tcggcgtggc gcgtccggta cttgaaatca cctgcccacc accggagctg
     6541 gtcagaaacg ctgtcaggat tatggagcat aaatcagggg ttgcccgttc agtatggacg
     6601 gcccgcctta atggctgcca gattatctgg cgttaataac ggcttattta tttgtgagga
     6661 ataacaaaat ggcaaaagta attattgaaa ttaaaaatac agtatctgga attaaaggac
     6721 tgaatttgcg caccagcatt gcggtggacg gtagtgcgga actggacggt gatgagggta
     6781 cacttgctgg catggtggcg ttactggtgc ttaataaaag ccagaaaata attaatgagt
     6841 cagcccatga agctatcgag attttgaaaa acgatagtgt catcaccggt ggccgtgtca
     6901 ctgaaatggc tgttgaaaaa acatgccact gaaaggaacg ctgacaatga acgtcaaaat
     6961 ccgaaatgaa atccgggcat taattcggat taaagagcgt aataacaatg gtggagactt
     7021 gcgcgaattt atttgtgcgc gcgaagttga ggggtatggc gaaaagactt acctgattgc
     7081 atttgaccat tacagcattt gtgcgcgtta ttgcggagag gcggtattcc gcgccattac
     7141 gtttggcaat gccttcaatg tggatttatg ggaatacgtc atggaccgcg aatacatctg
     7201 cgcgtcagac ccgaaagccc gtgaaatgtg gcagcgtatc tggcgcgatt accggttaat
     7261 ggcaaaaggc tgggcgcgct gctgttattc ctcgcttgtc ctgaaagcgg ttcagttatc
     7321 gctgcggcat attccggcat cactgcgcga gcctctgttg tactgagtgg tgaccggaat
     7381 gacgtgtaat cgtaaacgct ggtcacgcga agaccgggaa tttatcgaag ccagtgtcgg
     7441 aaaaatgacc gtcgaggaaa tggcgaaaaa actgaaagtt gccccaaccg ccctacaggc
     7501 acatgccaga aggcacggaa tatcgttgtg tgcataccgg gtcagtgagc acgacaaata
     7561 tttatgccgt gaactttata aagaagggct ggctattcat gtgatagcca aaaagatgga
     7621 attaagcaat cgtgctgtat ccggcattgt atacagtgaa tattaattaa caggagcttt
     7681 atttatggct aaacccgcaa aacgaatcag gaacgccgca gcggcttatg ttccgcaatc
     7741 ccgcgatgcc gtggtatgtg atatccgccg gattggggac ctgcaacgcg aagcagcacg
     7801 acttgaaacg gaaatgaatg acgctatcgc tgagattacg gagaaatacg cctcacagat
     7861 tgcaccgctt aaaaccagta tcgaaaccct ttcaaaaggc gttcagggat ggtgtgaagc
     7921 gaaccgtgac gaactgacga acggcggcaa ggtgaagacg gcaaacctgg taaccggtga
     7981 tgtgtcatgg cgtcagcgcc caccatcagt aagtattcgc ggtgtggatg cagtgatgga
     8041 aacactggag cgtcttggcc ttcaacgctt tattcgcacg aaacaggaaa tcaacaagga
     8101 agcgatttta ctggagccga aagcagtcgc aggtgtcgcc ggaattacag ttaaatcagg
     8161 cattgaagat ttttctatta ttccgtttga acaggaagcc ggtatttaat accacgacga
     8221 atatttaatt aattcacttt cttttaatta tggcgcgatg cgtcagggga ttgctcgcgc
     8281 ctgaatcagg accagaggaa atgaatatga aagcatatgc ttattttaaa ctgagtgacg
     8341 cagccgctaa cggttatacg attcgctggc tgaaagatgt ttctgttaag cgtgacaaaa
     8401 tcatctgctc attgcagcgt gcgtttggcg cagacggtgt ttcccttgtc agtaacagag
     8461 ggtatgaacg ggttgaatgt atctggatga atcaattagc gccggaatta tggcgtggta
     8521 aatcagacgg actgtttatt ggtggatttc agttttatgg cgttgtaccg gatataacaa
     8581 caccggaagg ccgaaacaat gcgacgctta ttcaggaaca tgaagagcaa ctccggaaat
     8641 atcccacatt cccggtctgg ttgtgtaatg aactcggggt tgatgttgta cccaacatgt
     8701 ttgatctcaa atcagtctgg acgatgcacc acagccgtga cggattcggc attttgtttg
     8761 aggtgtgcct gctggatggt gaggttaagg gaccggttcc tgccgaatgc cagcgcatta
     8821 aacattctga atttgtggca ctgacggagg aataaccggt gattgatgca aaagttctga
     8881 acggtgtcag cacattatta cgggcttacg ggcgtctgac ctgcggggtt ctggctgaaa
     8941 aaatgaatat gctgccctcg tcaatggtgt atttcctgcg tgatgcggtt gatgccggag
     9001 tgctcaccga atgcaacgga ttttatgacg ttccgcgccc tcgtctgacg tcacctgtaa
     9061 ggcgaaacac aacggagctg ccaggggttg atgatgctgc atggtgcacg ttccgtcgtt
     9121 ctttgccttg gctggaaggt aacgccattc cggcactggc aaaagaattt gcgacaggcg
     9181 tactgacctg cgagtcggtt tacatcgttg ctgaagtgga tgacgagatg tgcaaacagg
     9241 gaatgccccg ttttgtgatg gcgtatatcg atatccggct gggtcgtttt atttgtggct
     9301 ccagtggctg gaatatcacc gaccacgtcc tgcgctatct cattcttgat tattcaccgg
     9361 ttccggcgga aacacaggaa atcagcgggc atgattaagt caattggctt tgtcctgctg
     9421 gttggcacct gtgggaatga tgcctgcgat gccatacctg taacagaaaa aatctggccc
     9481 acagagcagg catgtatgca ggtgatggag cgcatacaaa aacgttatcc cggtgaaatc
     9541 ttttattgtg aggatgcatt aagaaatgag taaacgtatt tatgacgacc cgaaatatcg
     9601 ggctgaaatg cgggcgcaac tggttgtcag tggatatacg ccagagcagg ccgatgcaac
     9661 actgacggaa ctgatgggtg agcgtccatt tgatgaagct gaatatgaat actggctgaa
     9721 gcagttcagg ctggaaattc tggaaaacac cacactgcca tcgctgataa gaataataaa
     9781 aagcgttatc gacaatgcct gctacaacgg tgattgcctg gctgcagcaa ttgcgaccca
     9841 tgctctggtg aatctgctgg aggttaccgg cgcagttttc ctgaatccag aaaaggtatt
     9901 aagagatgaa aatacagatg attactgatg ctgatttaat ggctgcaacc ttaatttcca
     9961 gtggcatgtc agaacaaaag gtaaatgatt ttctggataa tgtcgtatac caacgctatg
    10021 agcaggccag agaaaattta atttcagaaa aacgcacgat gattgccgaa tcattaagtc
    10081 cagcctggat tattgaggaa ttacgaaaaa tcattgccag ccaggataaa gtagcggcag
    10141 ttgccgccct cgatgtgcta catcgaatga gtaaagagct attcaacacc gaattaattt
    10201 taaatccggg gggacataat gagccgcaca tccctgatta aattaattca tgttgcccgt
    10261 cgtgacctgc aactcgacga tgacacttac cgcacctttc tggtgcagtg cacgggcaaa
    10321 accagttgcc gcgagctttc cgttgcacaa ctggaacgtg tgctcgatgc catgaaagag
    10381 cgcggtttta agaagcagaa aaaacatccc cgccgtcgct ttaaggggca tgtcacaccg
    10441 cgcgagaaga tttataaaat ctggcagcag atgttccttg atggtttcgt ctccgatatc
    10501 agcgacgctg cgctggacaa atacgttgaa cgcctgacaa ccagacgtaa tggcggtcag
    10561 ggtgtttcca cgctggcctg gtgtcacggg gaatcgttgc aggttgtgct cgaaacgctc
    10621 aaacagtggc atatgcgctg catccgtgaa gcctttgccc gacatggtgt gcctttacct
    10681 gtgagcaaaa ccgggcagga gctgcgcggc tatgatgcac tgaccagtgc gtatgcccgc
    10741 gcacgaaaca gcgggagaat tgctgtatga aacagatgaa agagcaggat ttattcgaag
    10801 acttccggga cgacagcgtt ctggaatacc ttaacgatta ccaggaaaac acggcttatc
    10861 cggcgttact ttctgaactg aacgcactgc tgcgtaaaga gctggcccgg attggcgcag
    10921 accctgctca ttcccttgag ctggttgtgg cgatttgtcg ccatatcggc gggatgcagg
    10981 tttatgttcc gagggggaat attctggaga atcttgtccg ggatatgcgt atctggcgtg
    11041 attttaacgg ccataatatt cctgaactgg ttcagcgtta cggggtgacg tataaaaccg
    11101 tctacaaggc catcaaacgg atgcggcgtc tggagtgtaa taaataccag ccggatttgt
    11161 tttaccactc cccgaacctg cctcaccccg ttcgtcgtcc gccacgacgt ccttaatcaa
    11221 tgaagccggt aaatccggct ttttttatgc ctccggcacc atgaagcaga ccacgtttaa
    11281 ctctgcttca caggtgcttt tatggaaaat ctgaaattct ccccggcctt tgaacatgcg
    11341 ctgaatttta ttctgcgtcc cgatatcgaa ggcgtctatg tcaatgaccc cactgaccgt
    11401 ggcggcgaaa ccagatacgg catttctgac cgccgcgacg gtgtgattga cggcaaaacc
    11461 gatgtcagcg gcgacggtaa accggatacc cgcatcaggg atttaacccg cgaacaggtc
    11521 gcgcagattt actggcgcga ttactggctg cctgccggat gtgaccagtg gcctgatggc
    11581 gtggcgatgt ttgtgttcga tgcggcggtt cagcatggcg ttaaaaaagc tatcaggatt
    11641 ttgcaggaag ccgctgatgt ggatgctgac ggcatcatcg gtccgcgtac ccgtaaggca
    11701 gtgagcctgt ccaccccgga ctggttgctg acccgctgca ttgtccgtcg ttcccgcttt
    11761 tatgccgaca tcatcaaatc caaacctgcc cagggcaaat acctgaacgg ctggtttaac
    11821 cgcatggaga aagtgaccga cgcctgtctg gaaattatcg acaacgtgtc gtccgtcacg
    11881 cggggatgat atgggcaaag gctgggatgc ctcgctgaga gccgggcggc gtgaccgcct
    11941 gcgtcaggag gttttacatc gtatggccgg aggtccgccc ccgaaaccgc tggactatac
    12001 cggtcatgac ggcacacacg ccagccacta catgcgtggc tggaacaccg tggatacacg
    12061 ggacattttc tggcagtgcc agaagtacaa ggaaaaactc aatgtacaga atgaatagtc
    12121 tttttgttcg cgttatttac tcccgcttta tgttgtgcca gtggtcgttt ctggggctgt
    12181 cggcagtcct ttttctggcg agctgttttg ccggatggca ggcgtttgca gtcatggtgc
    12241 tggcatacgg cgcgacactc tgcctgattc acagtttcca gctgggcaac gtaccttatc
    12301 gcctgcaacc caaaaggcgt ttccgggccg cctcccttcg aattgtcgtg tggtcatggg
    12361 gggtctgggc cgttggtttt ttcatgctga cattcggcct tctttatctg aaggagcctt
    12421 atcagctggc gttctggctg ggcggtatct tttgtgcggc actttataaa catcagcgcc
    12481 gttttcataa gggggattca tggacccgtt aaccctttca ggcatcgcct ccgttctgct
    12541 gaaagccggg ccggggctga ttcgttccgt cgggcgctgg tttggtggca gcacatctgc
    12601 cgccgctgac tcggtggccg gtatggtgga aagcgtccgg gaaagcctgc cggtggctga
    12661 acagcaacgc attctggaac aaaaactggt gatgctgtca ccggagcaac tggtgcagct
    12721 ggaaaccctg aaagtccagc tgcaacagct ggaggtggag cggcagaagc tggtgctggc
    12781 tgaccagcag gccgctcacc acgaacaaca ggaaaccatc cgcaacggtg acaacgccac
    12841 ggacagctat gtgcgccaga cgcgcccgtt gctggcccgt ctgtcctgtt acagcagtct
    12901 ggcctatgtg ctgttgctct cctgtggcca gattgccggt gcggttgccg gtgcgaacgg
    12961 tatcacgctg catatgccgt caccggactg ggatatcacg ctgatgttgt ttaccccggc
    13021 gctgggctat ctcggggtaa gaacccttga cggctttgcc cgttacagca aatcgagctg
    13081 tcacaaaata tcagcggggc cgaaatgacc gatgaaaccg accgcatcag tgaagtggtt
    13141 ttaaacgaac gtcaggacgt cgttaacgcc tggctgatgc ggataaaaga agcaccccac
    13201 agtcggggct tctgcaatga ctgcggtaac gttattccgg cacagcgcat tgcggtgctg
    13261 ccggatgtgg tgacctgtat tgactgccag caggcgcggg aacgcaggag gaagacgtgt
    13321 cctgggaagt gatccgggcc aactggcctg ttttatgggc gctgttgatg acagcaataa
    13381 acctgattca gctgattctg gcgaagactt acgtcaaacg cgaagagttt gattcgctgc
    13441 gttcccgcgt gtcggtgatg gaaggcctgg tgaatcagct acccgaccgc aatgaatttc
    13501 accgcctgca actggatatc agcaacctgc ggggtgaaat caaagagctg gggccatcca
    13561 tccgccaggt atcccgcatc agcgatttgt tattagagaa cgaattaaag gaaaaaaatt
    13621 aatggcgatg aaagagatcc tcactgaaga ccgtcgtctg gtgttgctgc gttccctgct
    13681 ggactgtggc gacagcgcga atgaatccgt gttgcagacc tgccttcagg cctacggtca
    13741 taaggtatcc cgtgatgtgg tgcgcaccca gcttgcatgg ctgcgcgagc aggggctggt
    13801 tcgtctgtct gatgtgggcg gctgttacgt ggctgaaatc accggcagcg gcgaggatgt
    13861 ggcaaacggc ctgtccggtg tgccgggcgt taaaaaaccc cgtgcgaggg actgaacatg
    13921 gcccgaaaac tgaagccgtt aagtcgtgga gaacgggccg tcgtacggca gctggcgtac
    13981 tgccttgtgc tggcagacat tgagcaaaac gccattgtcc gtgcatacga aaaacataca
    14041 ggaaagccct ggaacccgga tgcaccggat acccccgtga aacgtgccct gcgttcatca
    14101 ccggcatatg cgcggctgtg gaagctgcca ggtaaggata ttcagtccgt tcgtgaagaa
    14161 atatacgctg gtctgaaaac acagaggaca gaagatggaa aatgaacaac gccccacccg
    14221 tggccgtctt tccaaagtgg atttactccc ggacagcatc cgtgagcagt tacaccagat
    14281 gctgcgggaa aagcggcaca cgcaggaaga aatccgcgaa gccatcaatg ccctgattga
    14341 cgaacacaac ctgccggggg agatgcagtt aagccggacg ggcttaaacc gctatgccag
    14401 ccgcatggaa aaagtcgggg caaaaatccg tgcctcccgc gaaatggccg aagtctgggc
    14461 ggcaaagctg ggttccgcgc cgacgtcaga cgtcggcagg ctgctgatgg agtttgtaaa
    14521 aacgctggcc tttgaaacgt ccatgtcgat gacagaagac gacaaacctg ttgcaccgaa
    14581 ggcgctgggg caactggcgc tggttgccca gcgtctggaa gcggcagcca tgaccagcca
    14641 taaacgcgaa aaagcaatcc gcgatgcgtt tgcgcaggaa atggcagaga aaaccgaaga
    14701 gctggtcaga gcgggcggtc tgtcaggcgg tgcggctgac accatcaaac gtgaaattct
    14761 ggggattagt gtatgacaca gatgaacgca ttcagtgaat ccgatgttct gctgccgtat
    14821 cagaaacgct gggtggcgga tgattcaaaa ctgaaaatcg ccgaaaaatc ccgccgtacc
    14881 ggtttaacct gggcggaagc ggcagatgcg gcactgaccg catcactgaa gaaggaaaac
    14941 ggcgggcgcg atcactttta catcggttcg aacaaggaga tggcccgcga atttatcgac
    15001 gccgtggcga tgtgggcaaa agcgtttaat gcagccgcgg aagaaatccg cgaggaagtg
    15061 atcaccgacg aagacaagga cattctgacg ttcgtcgtgt acttcgccag cggatttaag
    15121 gtcaaggcgc tgtccagtaa tccgggcaac attcgcggga tgcaggggaa tgtcaccatc
    15181 gacgaagcgg cgtttcatga aaagctggat gaggtactca aggcggtcac accactgacc
    15241 acgtggggcg gtaaggttcg ccttatctcc acccatgacg gcgtggacaa cccgtttaac
    15301 cagctgattc aggaaagccg cgcgggcaaa aaagattacc gtattcacac catcacgctg
    15361 gatgacgcct gtaatgacgg gctgtaccgg cgtatctgtc aggtgcgcgg catggtgtgg
    15421 tcaccggaag ccgaggtgga atggaaagaa ggcctgctgc gaaataccgc cacccgcgaa
    15481 gatgcgctgg aggagtatta ctgcgtcccg aaaaacggcg gcggcacgta tatcccccgc
    15541 tcactgcgtg aacgtgcggc ccgtggcact ggaaaagtgt tgcgctttac cggcacaccg
    15601 gaatttaacg cactgacgga aggccagcgc cgggcagaga tcagggaatg gctggaaacg
    15661 gtggtgcgcc ctgagctgga aaaactcccg aagaatctgc gccactgtct gggggaagac
    15721 tttgcgcgtt cgggtgacct gaccgtgctg gccccggtga cggtgaacga cgacaccacc
    15781 cgcgaggtgc cgtttctggt tgagcttgcc aatgtgccgt ttaaacagca ggagcaggtg
    15841 ctgttctggc tttgcgatcg tctgccccgt cgtgacggta tcaaaatgga tgcgcggggt
    15901 aatggtcagt atctggcaga acaggcggca gaacgatacg gcgacgaggt ggaacaggtg
    15961 atgctgtccg tggcgttcta ccgcgaaaac atgccccgtt tccgtgcggc gtttgaagat
    16021 gatgagctga tccttccgaa gcatgaagat gtgatcaatg acctcggggc cattcagtta
    16081 ctgcgtggcg ttcccggtat tgacgatgcc cgtactaaag gcagtgatgg ccgcaagcgt
    16141 cacggcgacg ccgctgtggc tattttcctg ggcttccttg ccagtaaaga tgactgccac
    16201 cgttacgagc tgcaccgcct gaaccgcccg gcgaaaccgg aagaaggcaa cgcgcgccgc
    16261 cagatgaagc tgacacgcgg cctgaaaaat gagggaggtt tactgtgaac ctgaaacaac
    16321 tggccggggc cgttcgccgt ctgctgaatc cggcaaccgg tgaggaagac acgctgaaaa
    16381 aagaggcgct caacgaagca caggcccgtc cgcgtcatgc cggtgtgcgt ccgacctcgc
    16441 cgggtatcag cgtcgcctcc gggctgaacc ccggcaggct ggcaggcatt ctgcgcaatg
    16501 ccgccgacgg catcacgcgt gattttttta tccttgccga agagatggaa gaacgtgatt
    16561 tgcattacgc ctcggtgctg cgcacccgca agctgacagt ggcaggtatc gaaccggtgg
    16621 tggtggccgc cagtgatgac gatgccgacg tgcagctggc ggacgccatc cgtgcgctga
    16681 tggaagcccc gcaaatcccc gaactgatgt ttgatttgct cgacgggctg ggaaaaggcg
    16741 tggcggtctg tgaaatcctg tgggacaccc gcaacaacca ctggatgccc cgtgattatg
    16801 agtgggttga tccccgtttt ctgaaagccg aaaagcccac gctgcgccag ttccgcctgc
    16861 tgacggacga tgaaccggtg gatggcgtac cgctgacacc gggaaaattc attgttcacc
    16921 agccgcgtct gaaatccggt ctgccgctgc gtaacggtct ggcccgtctg gtggctgtga
    16981 tgtacatgct gaaatccttt accgtgcgcg actggtgggc atttgcggaa aaattcggca
    17041 ttccggttac ggtggggaaa tacggtccca acgccacgga ggagcaaatc cgcgtgctga
    17101 ttgatgcgat tgcctccatt gcctcggatg ccgggtgtgc cattccgcag tccatgcaac
    17161 tggagatgca ggaaaccgcc agccggaata acggcggcgc actgttccgt gaaatggcgg
    17221 aatggtgcga tgcgcaaatc agtaaggctg tgctggggca gaccatgacc acggataacg
    17281 gcagttcgcg ttcacaggcg gacgtgcaca atcaggtgcg catggacatt gtgcgctggg
    17341 acgcgcgaca gctggctaac acgctgaatg aatatctggt gcggccctac atcgaggcca
    17401 actacgggcc acaggcgcac tacccccgtg ttgttctgcg tatcagtgaa gccgaagatc
    17461 tgaaggcgct gacggatgct ctggtgccgc tgattgaccg ggggatgcgg gttcaggaat
    17521 cggagctgcg ggaccggttc ggcctggctg agccagacga aggggctgat gttctgcatc
    17581 cggtttccgc cgggatgccg ggtgatatgg cgatgaaccg cgagcgcgtc gccctgaaca
    17641 gggaacaccc cgacgaactg gcacagatgg tggatgatgc cctgcgtgac tggcagaaaa
    17701 ccggcgaggc gttcacgaac ccggtgctga cactggcgca ggagtgcgac agttttgatg
    17761 actttctgaa acgcctgcct gagcttcagg aaacgctgaa cgcggacgac tttgccctgc
    17821 aactggcgga ggtgtgcttt aaggcgcgtg cgctgggaga cactgctcat gcgtgaaacc
    17881 ctcatcccga aagaggcgct ggcgtggctg aaggcgaaga agctgcgccc cggttttgat
    17941 taccgggatg tatggcggga agagcaccgg aacagcttca cggtggcaaa aatgctgcaa
    18001 ctggatttgt tgtcggatgt gaaagccctt gtggaagacg ccctgcaaag cgggcagacg
    18061 ttcagcgagt tccgggagat gctgcaaccg ttgctgataa aacgcggatg gtggggcgta
    18121 caggagatgg atgatccgct gacgggtgaa acccgcaccg tgcaactggg cagcgaccgc
    18181 cgcctccgca cgatttttga taccaacatg cgcaccgccc gcgcggcggg ccagtgggaa
    18241 cgcattcagc ggacaaagca ggccatgccg tatctgatgt acgagctggg gccatcccgt
    18301 gagcaccggg tggagcatgt gaaatgggcg cgtctgtgtc tgccggtaga tcatccgttc
    18361 tggcagacgc attttgcccc taacggctgg ggctgcaaat gcaccatccg tcaggtcagc
    18421 cgtggtgagt atgcgcaact ggcggcacag ggtaccattc acaccgaagc gccggaaatc
    18481 agaactgtcc gctgggtgaa caaacgcacg ggcgaagagg aagatgtgcc ggaagggatt
    18541 gatccgggct ggaactacaa tcccggtata aaccgtgagc aggagctggc gcgccagctg
    18601 gcggccagac aggcccgttt taacagtgaa taacccaccc cgccgtaaat acccctgaaa
    18661 cgcatcagaa acgcgttttt tattctgatg gcatgaatgt acaccctggc ttttttgaag
    18721 cgtctgtggc gtttttgaag gggttttgaa gggggtattt ccccgttttc agtgaagccg
    18781 gtaaatccgg ctttttttct gccttccgca tactgaccgt cggtaacccc agacgacgga
    18841 gactgacatg caaccggaac tgctggcgct gtgtttttcc ctgccagaac ccatccctga
    18901 gctgacaccc gctcaactgc cggaatggct tgaactcgtt cctgcgggtg agttcaccgg
    18961 gcgcgatggc cggacgtgga tgaaccgcaa tccacatgag gtggttgccc gttcgtccga
    19021 catcaacatt ccggtggaca ttgaacatgc caccgaaatt aaaggcccgc ggggtgaaga
    19081 ggctccggcg tatggctggg tggaagcact gcgggtgacg gacagcggca ccattgaagg
    19141 gcgtgttgtc tggagtgagt ccgcccggtg gatgctgagc gagcgccgct accgctatta
    19201 cagcccggcg tttttccatg acgcagacgg tgcggtgacg cgcctgtcca gcgtcgggct
    19261 gaccaacaaa cctaacctgg attttcctgc actgaatacg gagaaaaacc cgatgacagt
    19321 acctgtgcaa atcaccggcc tgcttgggct ggctgaatcc gccacggtgg acgataccgt
    19381 ggccgccatt aaacaacttc aggagaacga acaggtggcg ctgaaccgcg cacagacacc
    19441 tgacctgacg aagtttgtgc cggtggaaac ccacaatctg gcactgaacc gtgccgaaac
    19501 cgcagaaaaa cgccttcagc aactggaaga gaaggaagcc gaggcgcttg tggatgcggc
    19561 catcggggcc ggaaaagtcg ccccggcaaa ccgtgacatg ttccttgcca cctgccgcac
    19621 ggaagaaggc cgcaaacagt ttgcggagta caccaaaggt gcacagccgc tggttaacaa
    19681 cgacaagccc agccagggca aggataaacc cgcgcagaca ctgaccgatg ccgaactggc
    19741 gatgtgtcgc agcatgggta ttaccgggga agagttcctc gccgctaaac cgaaacagga
    19801 gtaagtaaca catgtcacag actatcagtt ctgaaatcct tcatgcactg accacctgcc
    19861 tgagcgccgc ctttacccgt ggactgagtg gtgtcgaacc gcagtggaat cgtattgctt
    19921 ctgaggtgcc gagttcgtcc gcctccaaca cttacggctg gatgaaagat ttaccggaca
    19981 tcaaagaatg ggtcggtgag cgtcagctgg caacgctgga cggttatggt tacaccatca
    20041 ccaacaaact ctgggaaagt tccattcgcg ttaagcgtga acacattgaa gatgaccaga
    20101 ttggtcagta cagcattacc gccgaacgct atggccgtat gactgctgtg ttcccggaca
    20161 agctgtgtta cgccctgttg tgtgccggtt ttaacaccct gtgctttgac ggccagaact
    20221 tctttgatga agaccacccg ctgggtgacg gtacgtacag caacgttgtc ggcaccccgg
    20281 catcagacaa gggcgaaccg tggttcctga ttgatgagtc gcaggtgctg aaacccatca
    20341 tctggcagac gcgacgcgcc tttaagtttg aagccctgga cgatctgaac agcgagcaca
    20401 ccttcaagaa ccacgagttc ctgtacggcg tggacggtcg ctgtaatgcg ggcttcggct
    20461 tctggcagac cgccgtcggt tcccatgcag cactgacggt ggagaactac aaaaaagcca
    20521 atgagttgct gcggggcatg aagggcacca acggtgagcc gctgggcatc cgcccgacca
    20581 cccttgtggt ggggccgaaa aaccgtgcgg acgcgaagct cattattgac gccatgctgg
    20641 ttaacggcgg cgattccaac atctggtaca aggatgtgaa gatcgtggac agcccgtaca
    20701 tcaccacccc ggcataaccc gtcatccgta aaccgcagtt aaaaggtgct gtgaatgccc
    20761 ttttaactgc cttttaaaag gcagagtcat gagtgaaaaa gcagaaacca aaggcgcgaa
    20821 agccgcaaag aacagcgctg cacaggaaaa cccggcaccg ctggcagacg ttattgtggc
    20881 tgacggtcag gcgaatgaac cacgtccggc tgaagacccg gttgctgtac agggtgacgc
    20941 ccctgtccgg ctgaacgtca gggccgtgtc tgaaaacggg ttctggcgct gtggccgttt
    21001 ctggtcacat atcggtgagg atgtggcggt gagcgctgcg gttgccaccc gcctgatggc
    21061 agagccgaat ctgattgtcc gggaagcgga gaaaggctga tgggatacat cacgcaggaa
    21121 gacctgttac gcgcggacgg caatcttgtc tggaacatgg cgattaaccg ggaaaccaac
    21181 gggctggatg aagacaaaat ccgtcaggcc atcagtgatg cggaagcgga aattgattcg
    21241 tttctgtccc gccgctacca gctgccgctg ggggtgacgg aaatcccgcg cccgctgcaa
    21301 cgcgtggcgg tatcgctggc gttttactgg ttatcagagc gtgacaatca aatcacggag
    21361 ctgatccaga aacgctacga cgacgccatt aaaaccctgc gtgaaatggc gaacggcacc
    21421 cgtgacctgg gcctgccgac gtatgccacc ccggcagaaa ccgacaacgg gaaaattatt
    21481 gtggtgggtg ccaatgcccg gctgttcacc cgtaacaacc tgaaaggggt gctgtgatgg
    21541 ggatttctgt acaggtcagc ggtgaccagc gtctggagga tatccgccgc gccgttgaaa
    21601 agctggcaga gggttcattg cagtcagagc tgctggaaag catcggtgcg gtggtggaat
    21661 cacagacccg ccgccgcatc atcgatgaga aaaccagtcc gggtggcgaa cgctggccgg
    21721 actggtctga cgggtacaaa aagacccgcc acggcaacca gagtctgctg cgcggtgaag
    21781 gccatctgct ggagagtatc cagtacatcg tggaaaaccg cgtggtgcgt atcggttcac
    21841 cgctggatta tgcccgcatc atgaatgacg gcttttccgg cagcgtgccg gtcagcgccc
    21901 acaaacggct tatctcgcag tgtttcgggc gggcgctgaa atatccggtc tggcagaccg
    21961 tcggtgcaca tcaccgcatg atgaacattc cgcaacgtga atttctgggg ctgtcttctg
    22021 ccagccagca ggagctgcaa caggttatca gtcatttctg gaaggaggtt ctgccatgac
    22081 agaacaacgc cctgaactgc gcacaccggg gagcaccgtt gcggccgcag agcgcattgt
    22141 agcctggctg cagacggccc ttcaggggga cacccccgac cgggctgatg tggtggagcg
    22201 tcacatcggc cagttcaaca gcacggatga agtgaaacgc tatctgtccg gtcgcaccgg
    22261 ctgtatccgg gtagctgccc tgcgtgtccg ggatatcaac ccgcgcggcg ggctgtccgg
    22321 tctggttacc tgggtggctt acatcatggc gacggattcg tgggggtatt cccgcgacgt
    22381 gcgctgtgaa gtgctggccg ggaaggtgat caaacgcctg ctgtcgtcgg atgccaccgg
    22441 gggcatgggg gctgaacgta tggccgctga tgtgcgggca gacaacattt actccgtcag
    22501 cctcgacggg ctgggtgtca ccatgtgggc ggtgacttgg gagcaggaat tccggctgga
    22561 tgaagagatt gatctcgccg cgctcccgga tttcctgcgc ctcggggcga cactgcgcag
    22621 cggcgaacac actgaaatta acgacgtgat ccatgtacgg ggtgacgatg gaacagaaac
    22681 tgattaagcc agcgcgggaa aacgtccgtg tccgtaaacc ggatggcgca catttatccc
    22741 cggaagggga acgtctcaac gtctgcgctt actggctgcg ccgtgaagcc gagggagatg
    22801 tggaaataac cacacttccg aaaaataaca acaaaaccag agggaaaaaa taatgtcgct
    22861 gggttccatt cctgatgata tccgcgtccc gcttgtctgg atcgatatcg acaattcaca
    22921 ggcgctggag ggtgcatccg cgcaaagccg aaaaattctg gtcatgggcc atgcggtatc
    22981 gtccggcagc gcagacgccc tgtcactgac ccgtatcacc agtgacagcc aggcagacca
    23041 gctttatggc aaagggtcga tgctggctga aatgctcaaa atgctgcgtc gtgccaacac
    23101 gtacacggaa acctgggcaa tgccggttgc cgcgcctgaa ggcgctgccg caaaagccac
    23161 gctgaccgtg ctgggtacag cgactgaagc cggaacggtg gcactgctga ttaatggtgt
    23221 gtccgttcag gtgagtgtga gcgccggggc tacgaaggaa aacattgcaa aagccattgc
    23281 tgatgcggtg acgaaaaagc ctgccacgca ggtggccgct gcggtgaagg atgatgccac
    23341 ggataccgtg gagctgaccg tgaactggca cggcgtcacc ggcaacggtg ccgacgttcg
    23401 cctgaactac cacaccggtg aagccttccc ggcaggtgtg aaggtgaccg caaccgcgtt
    23461 taccggcggc accgggacac cggaaatggc agacgccgtt gcggccatcg gcccggagtg
    23521 gtttaccgat atcatcgccc cgttcaccga cacgaaaagc ctgaacaccc tgcgtgatga
    23581 actgctgaac cgctgggggc cgctcaaaat gatggaagcg cagctgtgga cggcgtttcg
    23641 tggcacacac ggggaaaccg gcacgtttgg tgaaacccgc aatgactggc tgattagctg
    23701 tatcggcacc aacctgtcac cgcacccggc gtggatgtgg gccgcgtcat acggcgcaac
    23761 ggcagcgtat caccttgcca ttgacccggc gcgaccgctt cagacgctgg tcctgaccgg
    23821 cattctgccg cctgcgcgta atgttcgctg ggatatgccg gagcgtaatc tgctgctgca
    23881 tgacggcatt gccacgcaca tggtggacgc cggggataac gtctgcatcg agcgcgaaat
    23941 caccatgtac cgggttaacc agtacggtga tgcggatgtg tcgtaccttg atgtgcagtc
    24001 acccgccacg ctgggccgta tccgttacat catcaagaac cgtttcacga accgttatcc
    24061 gcgccacaag ctggcggatg atgacgtgct ggactcgctg gatgcggggc agccggtgat
    24121 gacgccgaag ctgtgcaccg cagaactgct ggatatctgc cagaccgaac ttctcccggc
    24181 gggccttgtg gagaacttca gcgattacaa agacacgctt caggtgacac gcgacagcag
    24241 cgataaaaac cgtctgaact ttatctgcca tccgaatctg gtgaaccagt tgcgtgtact
    24301 ggcaggcctg attcagttca agctttaagg gggccacatg gcaaaaattc ttggcatggc
    24361 gaccattcgc gtgaatggcc gcgaaattaa aaccgagggg aaatccacgc tgaatccggg
    24421 cggcttcagc cgcacccagc atatgggcgg cggcaaggtc tggggtatct ccagcaagat
    24481 ggcctcgccg tccatcaagg tgaccattgc ggcggcagcg gatatggacg tgattgaaat
    24541 cagcagctgg gaagatgtca cggtgatgtt ctacggcaac aacggcctga actacatgat
    24601 gacgggttca gccacggata acccggcgga gctggacgag gattccggca ccatcagtgc
    24661 gaactttatc ggtgaaaaat gcgtaaaggt gtgacatggc cgcgatggaa tttgaactga
    24721 aacacgggct tctgaccggc aaaggcacgg cagatgaaac cctgcataaa accgtgaaac
    24781 tgcgcgaact gaccgccagt gacgtgattg atgcgcaact ggccgcagaa cgcgtcgtca
    24841 tgggcgggaa cggaaaggcg gtggcctact gctcggaagt gctgatgggg ctggagatga
    24901 tgcgccgtca ggttgcgtca atcggtaata tccccggccc gctggacatg aaacagctgc
    24961 gaatgctcca cccggcagac cttgaactta tcagcacgaa agccgctgcg ctggatgaaa
    25021 tgcttgagga ggtggcaacg cgggggcgaa ctgatgccgc tggcggcggc tctgatgaac
    25081 ctgctggtta acctgtcttc gcgattcagc attcagtacc tggaacagct gcccctgcgg
    25141 cagctgttcc gcctgataaa gcaactggag aaacagcatg gcaacaggta accgtctcag
    25201 cacggaaatc atgatcaacc ttgccgggaa cctgaccgcc aaagcccggc agtacggcgc
    25261 aaacatgtcg cagttcgccc ggaacaacca gaaggccatg cgccttgtca aagccacaac
    25321 ggaggccgca ggacgcggcc ttgatacgct tggtaaccgc tacacggcaa tgatagccgg
    25381 gcttggcagt agtcttacca tcaaacaggt tgccgatttt gatgcgcaga tgcgccggat
    25441 gggtacggat gcccagttaa ccactgaaca ggtaaagacg cttcacgaca aaatcaggga
    25501 tgtatcgaac cagaaagata ttcgtattga tgcttctgcg cttggtcagg gggccagcga
    25561 acttctgggg aaaaccggtg attatcagta tgtggtggac aacctccgca acatggggtt
    25621 gatgatgcag gcgttcggtg ttgacggaca ggttgcagcc gggctgatgg cccagttctg
    25681 ggaaaaaaac gtccggggtg ctgatgcggt caataacatg atggacaggc tgtattcgca
    25741 gttcgcagtg gggtctgtaa gcgttgccga tgttgcccgc gctgccccga aactgttttc
    25801 catcattcag gatcagggac cggaagccat tgcccagatg ggggcatttg ctcaggtgtt
    25861 cgccaaaaac aaaggcagca ttgatgagac ggtaaccagt attcaggcca tgtatgcttc
    25921 cctgtcggac aaaaagaaca tcgagttcct gaaaaaaaac ggcattgacg tttttgtcaa
    25981 aggcacaaaa gacatcaaaa agccctatga gctgatgatg gaaatcctta aacgggccaa
    26041 atacgacccg ctgaaacttc aggatgtgtt tgatctgacc ggaatgcagg ggattaaggc
    26101 gctgcttaac cctgaaaacc ggcaactgat ggaacaaatg atttacggca cgattgagct
    26161 gggttccacg caaaaggccg cacagaccaa tgccgaaggc tttaatgccg caatacagtc
    26221 actgaataac gaatggcaac gttttgctga agggcaactg gcaaagcccg ttcaggacct
    26281 ggcggatgcg ctcaacagcg tggaccagaa cactgcccag aactggttgc aggtcggtaa
    26341 atatatggcg atcgcgctgg gcggcattat cgccatcaga aagacgtatc agttcggtaa
    26401 aaccatccac gacatcatga atcccaaagg gaaaggcaaa gggatacccg gcagcattac
    26461 ggatgttttc ggctccggtg tgatgccggt ttatgtggtc aatatgggca gtggcgggat
    26521 gaagggtaat accggcggtc tgccggatgc gccggattca tcgcgcaatc cccgtaactc
    26581 ccgcaaccca cgtgggccaa agacgccagc gctggttaca ccacaaactg catcaggatt
    26641 taatctcctt gacatggtgc tctctgcttt ccctaagaac aaagaggaag cagatgcgtt
    26701 aatcgcacgg gttcaggaaa acaacaaccg cccgacggta tggacggata tcaggaactg
    26761 gtttaactct ctggaaaaaa agaatattgc cgacccgcga ccgtgggctg gtatgcaacc
    26821 cactcagaat tatcctttcc ttccgcaaca attgcagggg gaaatccgtg tggtggtgga
    26881 aggtgatgcc cgcgtgaaaa gcgtcagagt ggatcaaccc ggcgtcagac tcagtgcgca
    26941 ggctggcgtc accagcgtgg agcagggtta atgacaacga gcaacggtaa atgggacggg
    27001 ctgcgcgacg cctcgtttcg tggcgtcccc ttctttctgg tggatacgga gggcaccggt
    27061 ggtcgtcgtg ccattccccg tgcttatccc cggcgtgaaa ccgcctggac ggatgataac
    27121 ggggccgttc cggggcaaca gcagattaac gcaaagctgc caggtaaaaa cttccgggat
    27181 gatttaaacg cccttttaga tgcgctcaat acccccggcc ccggcgagct tgtccacccg
    27241 tggttcggga tacagaccgt acaggttggc aaggtcaccc atcgcctcag cacggaggaa
    27301 gacggcattg cgtatgtcac ctttgaagtg tttgaggcag gcgagcgcct gttcccgtct
    27361 gcggcggata acacgcagca ggaagtgctg acaggcattg atgcggtgaa agccgccatt
    27421 gacgatggcg actggttcgg cgcactcgac gggctgggtg acatggccga cagttttctg
    27481 gccgatatgg aaaaccttgt ggctaacctg cccacgttac cggcagcact gaatcagtgg
    27541 atggacaggc tgaagcattt taaggcgatg gccggaaccc tcattgccac accggggcgt
    27601 ctggtcagtg agctgtcagc acgggtggaa gatattgcgg gccttgtgac ggaaccaccc
    27661 gaagcactgg cggtttacac gacattacgc aaccagtggg ccggagaacg tgccaggcag
    27721 attgccaccg gcgcactgcc ggaagatatc accgtgaagc ccggcagcgt ggcagacggc
    27781 aaccccggct ttgccatcgg gctgtcaccg gattatcagc cggtatctga cagcctgcaa
    27841 aagaacattg acgacttccg ccaggtggtt gtgctgggag ccctgctggg acaggccagt
    27901 gccgtggcct cgatgacgtt cgatacccgt gatgcggcgt tatctgccgg tgacacgctg
    27961 gcggctgaac tgcatgagca ggcggtggcg gcggtggaaa acaaccagcg gacattgtgg
    28021 cgaacgctgc gcgatttacg gcaggccgtg attacggatg cccgcgagcg tgccgcccgt
    28081 ctgccggaaa cccggcaggt gacgctgacc acaaccacct ctgccgcatt gctggcatgg
    28141 cgcgagcatg gtgacacacg ccgacgggat gaaatcgtgc agcgtaaccg cctgcgtcat
    28201 ccgtcattca tcctgccgac gcaacctgtg gagatcaccg actgatggaa tccgtgattc
    28261 ttaccgttga cggcaaactg tgggaaggct ggacggaaat gtccgtcagc cgttccctga
    28321 aggcgattgc cggtgagttt gaactcagtg tgacaacccg ctggtcagcg gcggcaccgc
    28381 gcgtgattcg tgaggggcaa ccctgcacgg tcaggctggg cgcggatacc gtgctgacgg
    28441 ggtatatcga tgattttatt cccggctatg acgctgaaaa cgtggagatt cgcgtcatgg
    28501 ggcgcgataa aaccggcgac ctggtggact gctctgtggt gcattcgtcc gggaaatgga
    28561 aaggcgtgcg gcttgaacag gtggcggctg atgtctgccg cccgttcggg ataaccgtca
    28621 tcacggaaac cccgaccggg gaggcatttg cgtctgtcgt tctggaacag ggtgaaacgg
    28681 gctttgaact gctcgaccgg ctggcaaaac agcgcggcgt tctgctgacg tctgacggcg
    28741 cgggtaacct gattatcacc cgcgcctctt ccgttcgcgc gggcgtgtca ctggtgctgg
    28801 gaaaaaatat cctcgccgcc cgtgggcgct tcagctggcg ggagcgtaac agccagtaca
    28861 tcatcaaggg caccaccagt gccggtggca aactgtggga cagccagcct gccccgatgg
    28921 tgggcggtcg ccagtacatc accgaagacc cggaaattaa ccgttaccgc ccgcgcattc
    28981 tggtcaatga agacagcctg accgtgggcg gggccagcat tcgcggggaa tggtacaaag
    29041 cccggatgct gggagagtcc cgcacgacgg aaatcacggt ggcaggctgg cgcgaacagg
    29101 gcgacagcgg cccgctgtgg cagaccaacc gccttgtcga tatcgacgac agcattcaga
    29161 acctgaaaac cacctggctg atatccgggg tgacatggac ggatggcgca caggggagga
    29221 tgacggttct ggcgctggtt ccgcctgaat cactggacat gccggaaatg aaagcgaaga
    29281 cgaaaaaaac aaaggcggtg gccacatggg attaaacgca attgcccgcc gcctgcgact
    29341 gatggttgac cgcgctgttg tccgcctggt gtctgacggt ctggcacggc agaacctgca
    29401 aattcagacg ctggcagatg ccaccgatga cgacgtggaa cgcttccaga actacgggtt
    29461 tacgtccgtt ccgccggaag gttctgaagc cattgtgctg gccgttggcg gacggcgtgc
    29521 cgggctggtt gccatcgcgg tggaagacaa gcgatgtcgc ccgaaaggac tgtcccccgg
    29581 cgatgtcagg gtgtatcacc gggacggcaa atcacacatc accctgaagg aaaacggcgt
    29641 cattgaaatc acaggagaac aggtagacgt ttcagggaaa acggtaaatc tcaccgccga
    29701 cgaattgctg aatattatcg gtaaacaaat gaaattcgtc ggtccctgtg aatttactga
    29761 agatgtcaga atcaagggta aatcattcag cgaacacatt cataaggacg gcgacaatga
    29821 gaagacatca ccgcccgtat gacgacagaa atccgctgga ataacatgct ctcacggggt
    29881 gacatcaccg tcacccataa cggcctctca cgggatgagg ggctggtcac tcaggttctt
    29941 atctgccttt tcaccgatgc ccgcgctgat gacgatgatg tcattcctga cggctccggc
    30001 gacccgcgcg gctggccggg cgacacgtac agcgattttt cgtggggttc ccgcctgtgg
    30061 ctgcttgagc gtgaaaagct gacggaagat gtccgcctgc gcgtggaaga ttacgcgcgg
    30121 ctgtccatgc agccgcttct gcgtgccggt tacgcccgta acgccaccgt cacggccagc
    30181 atcatcattc ccgaccgtat tgcctttctg gtggtgttaa cccgcccgga caaaaccaca
    30241 ctcagaatcg aaatcacccg tcgatgggag gccaccatta atgccgtatg aaatccccac
    30301 gctgggcaaa ctgattgccg acggcgaaaa agatattgcg tatgaactcg gcctgcaaaa
    30361 gctgccgcct gtcagtgttg agcaggcgct gaacgtgtca ttcagcagtc aggtccggga
    30421 tttatacgac catcagagct ggattaagga ccagataatc ccgtccacca cgtctgatga
    30481 tgaaaccatt atcaaaacag caaagtatga aggggttatc cgcaagcagg ccacctttgc
    30541 cagcgggcgg gtgaccttca ccagtcagtc cccccttccg gcagaaaccc ggatgcagtc
    30601 cgacacgaac caggtgtatc aggtactcac gtccggcgag gtgcaggacg gcgaggtcac
    30661 cgtcatcgtg caggctgaag aagccggtgt ggcgggcaat cttgctgccg gtgccgtact
    30721 caccctgctg tccccgctgc ccggaacggc cagcacaggc gcggtggctg aaggtggcat
    30781 caccggtggc gcagacactg aatccatcgc agcgctgctg gaccgtctgc tgtatgtgcg
    30841 ccgcaatcca ccgacaggcg gcgcgttgca tgactacgtg atatgggcgc gtgaagtgcc
    30901 gggcgtcagc cgcgcgtggg cgtgggatgc atggcacggc cccggcacgg tcgggctggc
    30961 atggctttat gatgaccggg aaaacatcgt gccaacccgc gaagacctga agacaatgga
    31021 gcagtatctg ttctgccaca aacacccggc cacgggcgtg atggtgggca aaccgggcgg
    31081 cattgaggtc tggccggtac aggtcaggct gaagaaagtc agcatatcca tccgtctgac
    31141 accggacagc ctggcgaaca ggagcgccgt ccgggcaaac ctgaccgcgt tacagaagac
    31201 actggcaccc ggtcagacgc tgccggtgtc ctcgctgcgc acggccatcg gtatgacgtc
    31261 cggcatcacg gattacttcc ttaacatcgg ggaggacacc accagtgatg tggatgagct
    31321 tatcaccatc ggggaggtga catggctcac agcgtgacgg aatggctggc cgcactgcaa
    31381 caggtcatgc cacggggtaa ggcatggccg cgtgataacg acgcggattt aaaccgcttt
    31441 ttaagggcgc ttgcagaacg tttaaaccgc actgaatacg acgcctcgcg tctgcatgtg
    31501 gagatgcgcc cggaaaccac gctccggctg ctgccggagt gggagcagta tctggcgctg
    31561 ccggaatgcg gaattgcccc caccaccagg gaagcccgcc gccgtgccgt ggtggagaaa
    31621 taccgtcgca ggggcgggct ggcaacctgg cagactgaag ccgcagcagc ggcgctgggc
    31681 ttcaccatta aggtgacagc cgttctgccg caccactgtc tgcgcgactg catgtatccg
    31741 ctgcatccgg cgcggtatcg ctggatgctg aaagtgaacg tcccgggtaa agatgccggg
    31801 cggtttacct gtattgatgg cgtcatgaca ccattaatca gcgaacggac ccgcgagctg
    31861 gaatgcctgc tgaagcatta ccggctggcg ggcacggaat atgaatatta ttacaccgga
    31921 gaataattta tgttttatgt ggataacccg acgggtgtgc cggtaatgcc acctgtcgcg
    31981 gcagaattaa gcaaaacaac gctttatttc accgaaggcg ggaacggtat tccgcccacc
    32041 tatccggggc cggactggtt taatattatt cagtctgaat tactggaaat actccggcag
    32101 gcaaatatca aaccggataa aaacacaacc gaccagatta tgacggcgct gaaaaagctg
    32161 tttattacga acagtggttc agccggtgct attgccggat taaccggtca gaataataca
    32221 ttcccgtatt ttaccggcaa agacacaatg gcattaacac cgctcagtgc ttttgtgcgc
    32281 ggtattctcg gcaaagagag tgcaacagat tttgcagatg cactgaaagt cataaaacaa
    32341 tcaggcggca cgatgacagg agagctgaaa atccgtggtg ttaatgcgct gaggattttc
    32401 aacgaagctt ttggcctgat ttttcgtcgt tcggaagagt gcctgcacct tattcccacc
    32461 agtgaaggtc agggcgagaa tggcgatatt ggtcccctgc gcccgttcac cattaatctg
    32521 cggacgggtg aaatatccat gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat
    32581 ggtgcgctgg gtatcggcgt tcagaacgcc ctggggggga attcaattgt tcttggtgat
    32641 aatgacaccg gatt
//
LOCUS       DAZPEP010000033        32648 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZPEP010000033 DAZPEP010000000
VERSION     DAZPEP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.033"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            282..1655
                     /gene="aroP"
                     /locus_tag="ACHUT9_001518"
     CDS             282..1655
                     /gene="aroP"
                     /locus_tag="ACHUT9_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFR1348024.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1698..2552)
                     /gene="ampE"
                     /locus_tag="ACHUT9_001519"
     CDS             complement(1698..2552)
                     /gene="ampE"
                     /locus_tag="ACHUT9_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFR1348025.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2549..3100)
                     /gene="ampD"
                     /locus_tag="ACHUT9_001520"
     CDS             complement(2549..3100)
                     /gene="ampD"
                     /locus_tag="ACHUT9_001520"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFR1348026.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3188..4081
                     /gene="nadC"
                     /locus_tag="ACHUT9_001521"
     CDS             3188..4081
                     /gene="nadC"
                     /locus_tag="ACHUT9_001521"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFR1348027.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4284..4724
                     /gene="ppdD"
                     /locus_tag="ACHUT9_001522"
     CDS             4284..4724
                     /gene="ppdD"
                     /locus_tag="ACHUT9_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFR1348028.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            4734..6119
                     /gene="gspE"
                     /locus_tag="ACHUT9_001523"
     CDS             4734..6119
                     /gene="gspE"
                     /locus_tag="ACHUT9_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFR1348029.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVNQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRQQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6109..7311
                     /gene="hofC"
                     /locus_tag="ACHUT9_001524"
     CDS             6109..7311
                     /gene="hofC"
                     /locus_tag="ACHUT9_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFR1348030.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPTLLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7346..8389)
                     /gene="guaC"
                     /locus_tag="ACHUT9_001525"
     CDS             complement(7346..8389)
                     /gene="guaC"
                     /locus_tag="ACHUT9_001525"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFR1348031.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8545..8589)
                     /locus_tag="ACHUT9_001526"
     CDS             complement(8545..8589)
                     /locus_tag="ACHUT9_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348032.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8614..9234
                     /gene="coaE"
                     /locus_tag="ACHUT9_001527"
     CDS             8614..9234
                     /gene="coaE"
                     /locus_tag="ACHUT9_001527"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFR1348033.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9234..9977
                     /gene="zapD"
                     /locus_tag="ACHUT9_001528"
     CDS             9234..9977
                     /gene="zapD"
                     /locus_tag="ACHUT9_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFR1348034.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9987..10184
                     /gene="yacG"
                     /locus_tag="ACHUT9_001529"
     CDS             9987..10184
                     /gene="yacG"
                     /locus_tag="ACHUT9_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFR1348035.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10284..10673)
                     /gene="mutT"
                     /locus_tag="ACHUT9_001530"
     CDS             complement(10284..10673)
                     /gene="mutT"
                     /locus_tag="ACHUT9_001530"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFR1348036.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10733..13438)
                     /gene="secA"
                     /locus_tag="ACHUT9_001531"
     CDS             complement(10733..13438)
                     /gene="secA"
                     /locus_tag="ACHUT9_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFR1348037.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13500..14012)
                     /gene="secM"
                     /locus_tag="ACHUT9_001532"
     CDS             complement(13500..14012)
                     /gene="secM"
                     /locus_tag="ACHUT9_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFR1348038.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(14243..15160)
                     /gene="lpxC"
                     /locus_tag="ACHUT9_001533"
     CDS             complement(14243..15160)
                     /gene="lpxC"
                     /locus_tag="ACHUT9_001533"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFR1348039.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15261..16412)
                     /gene="ftsZ"
                     /locus_tag="ACHUT9_001534"
     CDS             complement(15261..16412)
                     /gene="ftsZ"
                     /locus_tag="ACHUT9_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFR1348040.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16473..17735)
                     /gene="ftsA"
                     /locus_tag="ACHUT9_001535"
     CDS             complement(16473..17735)
                     /gene="ftsA"
                     /locus_tag="ACHUT9_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFR1348041.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17732..18562)
                     /gene="ftsQ"
                     /locus_tag="ACHUT9_001536"
     CDS             complement(17732..18562)
                     /gene="ftsQ"
                     /locus_tag="ACHUT9_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFR1348042.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18564..19484)
                     /gene="ddlB"
                     /locus_tag="ACHUT9_001537"
     CDS             complement(18564..19484)
                     /gene="ddlB"
                     /locus_tag="ACHUT9_001537"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1348043.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19477..20952)
                     /gene="murC"
                     /locus_tag="ACHUT9_001538"
     CDS             complement(19477..20952)
                     /gene="murC"
                     /locus_tag="ACHUT9_001538"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFR1348044.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21006..22073)
                     /gene="murG"
                     /locus_tag="ACHUT9_001539"
     CDS             complement(21006..22073)
                     /gene="murG"
                     /locus_tag="ACHUT9_001539"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFR1348045.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22070..23314)
                     /gene="ftsW"
                     /locus_tag="ACHUT9_001540"
     CDS             complement(22070..23314)
                     /gene="ftsW"
                     /locus_tag="ACHUT9_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFR1348046.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23314..24630)
                     /gene="murD"
                     /locus_tag="ACHUT9_001541"
     CDS             complement(23314..24630)
                     /gene="murD"
                     /locus_tag="ACHUT9_001541"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFR1348047.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24633..25715)
                     /gene="mraY"
                     /locus_tag="ACHUT9_001542"
     CDS             complement(24633..25715)
                     /gene="mraY"
                     /locus_tag="ACHUT9_001542"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFR1348048.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25709..27067)
                     /gene="murF"
                     /locus_tag="ACHUT9_001543"
     CDS             complement(25709..27067)
                     /gene="murF"
                     /locus_tag="ACHUT9_001543"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFR1348049.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLNAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27064..28557)
                     /gene="murE"
                     /locus_tag="ACHUT9_001544"
     CDS             complement(27064..28557)
                     /gene="murE"
                     /locus_tag="ACHUT9_001544"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFR1348050.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28544..30310)
                     /gene="ftsI"
                     /locus_tag="ACHUT9_001545"
     CDS             complement(28544..30310)
                     /gene="ftsI"
                     /locus_tag="ACHUT9_001545"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFR1348051.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30326..30691)
                     /gene="ftsL"
                     /locus_tag="ACHUT9_001546"
     CDS             complement(30326..30691)
                     /gene="ftsL"
                     /locus_tag="ACHUT9_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFR1348052.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30688..31629)
                     /gene="rsmH"
                     /locus_tag="ACHUT9_001547"
     CDS             complement(30688..31629)
                     /gene="rsmH"
                     /locus_tag="ACHUT9_001547"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFR1348053.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31631..32089)
                     /gene="mraZ"
                     /locus_tag="ACHUT9_001548"
     CDS             complement(31631..32089)
                     /gene="mraZ"
                     /locus_tag="ACHUT9_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFR1348054.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT         7758 a         9159 c         8052 g         7679 t
ORIGIN      
        1 ttttgatttt taagtcaata aaaacgaatt taaattcatt ctgcatattg agaggggttg
       61 aggctgagct ttacaaacgg tttcttttta agcaactcat cttcaaccat gcataaagcg
      121 ggtgcattcg ctgccgcata ccattattct tgatctgacg gaagtctttt tgtaacaatt
      181 caaacttctt tgatgtaaaa aaattaatac aacaaacgga attgcaaact tacacacgca
      241 tcactgcgta gatcaaaaaa acaatcaccg cacgaggttt catgatggaa ggtcaacagc
      301 acggcgagca gctaaagcgc ggccttaaaa accgccatat tcagcttatc gcgctgggtg
      361 gcgcgatagg gacagggcta ttcctgggta gcgcctccgt aatacagtcc gcagggccag
      421 ggattatcct gggttacgcc attgctggtt ttatcgcctt tctgatcatg cgtcagctgg
      481 gtgaaatggt ggtcgaagaa cctgtcgcag gctcctttag ccactttgct tataaatact
      541 ggggcagttt tgccggtttc gcctctggct ggaactactg ggtactgtac gttttagttg
      601 ccatggcaga gctgactgcc gtgggtaaat acattcagtt ctggtatccg gaaatcccaa
      661 cctgggtttc tgccgccgta ttctttgtgg tgattaacgc catcaacctg accaacgtta
      721 aagtgtttgg cgagatggag ttctggtttg ccattatcaa agttattgcg atagtagcga
      781 tgatcatctt cggcggctgg ctgctgttca gtggcaacgg cggcccgcag gcgagcgtta
      841 gcaacctgtg ggatcaaggt ggtttcctgc cgcacggctt caccggactg gtgatgatga
      901 tggcgattat catgttctcg ttcggtggtc tggaactggt ggggatcacc gcagcagaag
      961 ctgataaccc ggagcaaagt atcccgaaag cgactaacca ggttatctac cgcatcctga
     1021 ttttctatat tggttcgtta gccgttctgc tctcactgat gccgtggacc cgcgttaccg
     1081 ccgataccag tccgtttgtg ctgatcttcc acgagttagg cgataccttt gtggcgaatg
     1141 cgctgaacat cgtggtactg actgcggcgc tctccgtgta caacagctgc gtatattgca
     1201 acagccgtat gctgtttggt ctggcacaac agggtaatgc gccaaaagcg ctggcgtctg
     1261 tcgataaacg cggcgtaccg gttaacacca ttctggtgtc tgcgctggtt acagcattgt
     1321 gcgtattgat taactatctt gctccggaat ccgcatttgg cctgttaatg gcactggtgg
     1381 tatccgcact ggtgatcaac tgggcgatga tcagtctggc gcatatgaag ttccgtcgcg
     1441 ccaagcagga acaaggcgtg gtaactcgct tccctgctct gctttatccg ctgggtaact
     1501 ggatctgcct gctgtttatg gcggcggtac tggtgattat gctgatgacc ccaggaatgg
     1561 cgatttcggt atacctgatc ccggtatggc tgatcgtgtt aggtatcggc tatctgttta
     1621 aagagaaaac cgccaaagcc gtaaaagcgc attaatctct ctacgccctc acccatgcag
     1681 ggtgagggca ataatcttta caccaacgct ccgtaaatcg tcagtagcgc aatcaccacc
     1741 acgaccacga acgaggtttt cttcgccatt gaaaccgctg ccttcggcgt ctccacctta
     1801 tcgacatgcg gttcacgcgc cagagagaac tgcgccagac gcgttaacac ctgatactgc
     1861 gaagtatgga agtcacccag cgaagcaaac caggccggta acgctttctc cccatgaccg
     1921 atcaaggcat ataccacacc cgcaagacga accggcaccc aatccagtac atgaagcacg
     1981 gcatcaatgc cggactgtaa acgatgatgc ggcgtctggt atcgcgccag ccagtattgc
     2041 catgcacgca aaaacgcata ccccatcagc gtaacgggtc cccaggttcc ccccacaatc
     2101 aaccagaaca gcggtgcaag ataaaaacga aagttaatcc acagcaatgc attttgcagc
     2161 tcacgcaaat actcacgttc gtcgcagcct gccgggacgc cgtgaatcat ggtgagttcg
     2221 ccagccatcg tggcacgggc atggctatca ttacgtgaag cagctgtcag ataagcatga
     2281 taatgaagac gaactttacc tgcgccaata cacagcaaac caatcagcag ccacaccagc
     2341 agcgtgggaa cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa agtcacgccc
     2401 atcgcaataa tggtcatgcc taacgtgcgc ccgagagaaa aatgtttcac ccgccgaaag
     2461 aacgcttcaa gacgatgatc aagctgccag tgctcgccca acttaaacag gcgctcgaaa
     2521 attaacacca gtaaggttgt aaatagcgtc atgttgtctc cttgctgacc agcgcacgaa
     2581 accgtgccca atcaaatgca ggaccgggat cggttttccg atccggcgca atatcacaat
     2641 ggcccgtcat gttattagcg atatcgggat agcgatctat cagtgcccgc gtaaccgccg
     2701 caagctgttg atactgcgca tcggtatacg ccagagtatc ggtgccttca agctcaatcc
     2761 caatagaaaa atcattgcag cgttcacgcc cctgatactg agagactccc gcatgccaag
     2821 cacgtttatc gaaaggaaca tactggacta tttcaccatc acggcgaatc aaacagtgag
     2881 cggagacgcg caaatgggcg atctcagcaa agaaaggatg tgcctgcgga tcaatagttc
     2941 cagtgaataa tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg ctaatattgt
     3001 gcaccaccag cagggtgggt gtttcgtcat ccgggcggca atcgtaatgt ggtgagggaa
     3061 cgcggcgcgc gccaaccagc cacccctgtt ctaacaacat gcaggatctc cttatatgtg
     3121 gtgctaatac ccggttcaga gtagcatgtt tctaccttat gattcgttag ctatctggag
     3181 ttttaacatg ccgcctcgcc gctataaccc tgacacccga cgtgacgagc tgctggaacg
     3241 cattaatctc gatatccccg gcgcggtggc ccaggcgctg cgggaagatt taggcggaac
     3301 agtcgatgcc aacaatgaca ttacggcaaa acttttaccg gaaaactctc gctctcatgc
     3361 cacggtgagc acccgcgaga atggcgtatt ttgcggcaaa cgctgggttg aagaggtgtt
     3421 tattcaactg gcaggcgacg atgtcaccat aatctggcat gtggatgacg gcgatgtcat
     3481 caacgccaat caacccttgt tcgaacttga aggcccatcc cgcgtgctgt taacgggcga
     3541 acgcactgcg cttaattttg tgcaaaccct ttcaggagtt gccagtaagg tacgccacta
     3601 tgtcgaatta ctggaaggca ccaacacgca gttgttggat acgcgcaaaa ccttacctgg
     3661 cctgcgttca gctctgaaat acgcggtact ttgcggcggc ggagcgaatc accgtctggg
     3721 gctttctgat gccttcctga tcaaagaaaa tcatattatt gcctccggct cagtgcgcca
     3781 ggccgtcgaa aaagcgtcct ggctgcaccc ggatgcgcca gtagaagtcg aagtagagaa
     3841 tctggaagaa cttgatgaag ccctgaaagc aggagccgat atcatcatgc tggataactt
     3901 cgaaacagaa cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc tactggaagt
     3961 gtctggcaac gtcactgaca aaacactgcg cgaatttgcc gaaacgggcg tggactttat
     4021 ctccgtcggt gcgctaacta aacacgtaca agcactcgac ctttcaatgc gttttcgcta
     4081 attgctgaca gatttatccg ctccatcgcg ggcggataat ctccccttcc ccaactttct
     4141 tcgtaacgcc tcgcaaattt cttgtctttt gctgcaacga aataaaaaac ctcggaagct
     4201 gctttcccgt ttcgtttttt gcccctcgct tgtgctgatg gcgtttgcca aagtagcgcc
     4261 aaccaaatca aggagcgaaa cagatggaca agcaacgcgg ttttacactt atcgaactga
     4321 tggtggttat tggcatcatt gccattttaa gcgccattgg tattcccgct tatcaaaact
     4381 acctgcgcaa agctgcactc accgacatgc tacaaacctt tgtgccttac cgtacagccg
     4441 tagagttgtg cgcgctggaa catggtggat tagatacctg cgacggtggc agcaatggca
     4501 ttccctcgcc taccaccacc cgctatgttt cagccatgag tgtggcaaaa ggcgtggtgt
     4561 cgctgaccgg gcaagaaagt ctcaatggac taagcgtcgt catgacaccg ggttgggata
     4621 acgcaaacgg cgtcaccggc tgggcgcgca actgcaatat tcaaagtgac agcgcattgc
     4681 agcaagcctg cgaagatgtc ttccgctttg atgacgccaa ctaaggagcg gcaatgaata
     4741 ttccacagct caccgccctg tgtctgcgtt atcagggagt cttgctggat gccagcgaag
     4801 aggtggttca tgttgcggta gtcgatgcac cttcgcatga gttgctggac gcattgcatt
     4861 tcgctaccac caaacgtatt gagatcacct gctggacgcg ccaacaaatg gaaggccacg
     4921 ccagtcgcac acaacagaca ttgcccgtag ctgttcagga gaagcatcag cccaaagcag
     4981 agttgctgac tcgaacgtta caatctgcgc tggaacaacg tgcgtctgat attcatatcg
     5041 aaccagcgga caatgcctac cgcatccgct tgcgtatcga cggcgtattg catcctttac
     5101 cggatgtttc accggatgcc ggagtcgcat taaccgccag attaaaagtg ctgggaaacc
     5161 tggatattgc ggaacatcgc ctgccgcagg acgggcaatt cactgtcgaa ctggcaggaa
     5221 acgccgtctc atttcgtatt gcgaccttac catgtcgggg tggtgaaaag gtggtattaa
     5281 ggttgttaca gcaggtgaac caggcactgg atgttaacac gctgggaatg cagccgttac
     5341 aactggcgga ctttgctcat gccttgcaac aaccacaggg actggtgctg gtaactggcc
     5401 ctacaggcag cggcaaaacg gtcacgcttt atagtgccct gcaaaagctg aataccgctg
     5461 acattaatat ttgtagcgtc gaagatccgg ttgagatccc catagccgga ctaaaccaga
     5521 cgcaaatcca tccgcgtgcc gggctcacct ttcagggcgt tttgcgtgcg ttattgcgcc
     5581 aggatcctga cgtcatcatg atcggagaga tccgcgatgg cgaaacggca gaaattgcca
     5641 ttaaagccgc gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg
     5701 aaacgctggt acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta
     5761 cgctggtaat agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag
     5821 gggagcccat ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac
     5881 ccggttgtgt acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc
     5941 ccataacgcc ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa
     6001 cgcacgcacg acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc
     6061 aaggcttaac cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag
     6121 caactctggc gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatgg
     6181 gcagagagcc gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc
     6241 ctgaagcgaa tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt
     6301 catcaactgg cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg
     6361 gcggaacagc atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc
     6421 gaacagggca ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc
     6481 tatcaggcga tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa
     6541 ctggcgcgtc agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt
     6601 tatcccatca tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt
     6661 ctgccggagt ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag
     6721 gggatcatga cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc
     6781 tttctgctgg cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg
     6841 cagaaattgc tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg
     6901 cagatcttta cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta
     6961 gagagcgtca gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag
     7021 cacgatatca gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc
     7081 ccgctctgtt tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta
     7141 gacaacctcg cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc
     7201 ttactggaac cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg
     7261 atgtatctgc caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt
     7321 gttgctccac accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct
     7381 gcacacggat aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg
     7441 tacaagctga acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca
     7501 gcggcagctt aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac
     7561 gtttcatcgc agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct
     7621 caacgatgcg accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat
     7681 cggcaccgcc gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga
     7741 cgatcattcc gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg
     7801 gataaccgac gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt
     7861 taacgatatc agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc
     7921 cagcacaaat ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt
     7981 gttcggaata accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga
     8041 gaatctgttt agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt
     8101 tcagcacatc agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt
     8161 tatgcacagc agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta
     8221 cggtgtccat atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga
     8281 aggtgaattg acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc
     8341 ggatgagaac gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc
     8401 tggggttaat ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat
     8461 caatgcggcg caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct
     8521 ggtaaatagt gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt
     8581 gataatcatg atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga
     8641 ggcattggca gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc
     8701 attgatgccg atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc
     8761 attgctgatc actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc
     8821 ttgcgcgagc ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat
     8881 ccgctgattc agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg
     8941 tgggttgtgc cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg
     9001 gtggatgtca gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc
     9061 gagcatgtcg aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca
     9121 gatgacgtca ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg
     9181 cacgcacact atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga
     9241 cccaggtcct ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt
     9301 ttttgattca gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt
     9361 tcttccgtaa tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc
     9421 tgttgaaaga acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg
     9481 tggaccagag ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa
     9541 tttccgcgcc gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc
     9601 agcgactgag catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc
     9661 tgcatctacc ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc
     9721 cgctcaccca ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta
     9781 aacaaaccag tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc
     9841 tgaatctgtc gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg
     9901 ccattcgttt tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg
     9961 aactggcctg ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct
    10021 gcgggaaaac ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt
    10081 gtcagctgat cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg
    10141 atctttccga aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg
    10201 cacgtaggcc tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat
    10261 gcggcgtgaa cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg
    10321 gctggcggaa aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct
    10381 tctttacccc acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc
    10441 ctgtccggga attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg
    10501 acttcttcct gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta
    10561 ccgccgggaa actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag
    10621 atttcattgt tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta
    10681 aacctataaa aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca
    10741 ggcgaccatg gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc
    10801 ctactttgcg ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct
    10861 gatggctaag ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca
    10921 gctcctcaac ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat
    10981 attttaacga ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct
    11041 tcggatcttt ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg
    11101 ctaggtgctc tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac
    11161 gcatcatctc agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca
    11221 gaattcgctc acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa
    11281 ttggcaaatc gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca
    11341 tttcttccag tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac
    11401 gaatgctgtt gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt
    11461 aaatggcgcg acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt
    11521 cgaagttacg gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt
    11581 gttcaatggc ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg
    11641 aagcaaaaat acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat
    11701 ccccctgacg accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct
    11761 cggtaccgat gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt
    11821 cggctttaat tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct
    11881 gccagctacc accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca
    11941 ccgcagccgg ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt
    12001 tcaggacgtt gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt
    12061 cgatggagat agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt
    12121 caatgatcgc ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac
    12181 gaatcattgg acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt
    12241 cgaaagcttc ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt
    12301 agttctggaa ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt
    12361 tcgcttccac agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg
    12421 tgtgttcgtc aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa
    12481 acagcgcatg agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag
    12541 agtacagaga ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca
    12601 ccagaccacg ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt
    12661 cgccctggaa ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat
    12721 tcacgcgttt atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac
    12781 gcgcttcatc gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct
    12841 gtacacgttc ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt
    12901 tgttcgtacc gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc
    12961 ccggcaggtt gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg
    13021 cgtcacgttg cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg
    13081 cgttcaggta agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg
    13141 cgatgcagcg ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac
    13201 caaagacgcg cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca
    13261 gcacttcgcc tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt
    13321 cggagagttt ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc
    13381 ggcgcagggt gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa
    13441 taaaatctca aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt
    13501 taggtgaggc gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg
    13561 ctgaattttg cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc
    13621 gtgccttcct gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga
    13681 agaggcaaag attcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga
    13741 cggattaccg tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg
    13801 cggcgtgtgt tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg
    13861 tggttgcggg ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc
    13921 aaacctaaac tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca
    13981 aactgtcgcc agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac
    14041 gtatttagcg cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag
    14101 ttgtgcggaa aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa
    14161 ttcaaaaaaa aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt
    14221 ttatacgaca gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg
    14281 gcagttctgc gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag
    14341 cctgcagcag tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa
    14401 tattgtgacc acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt
    14461 cgtcttcaaa acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac
    14521 aatcgaagct gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga
    14581 aaccgaacgt acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg
    14641 catagcgctg gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg
    14701 aaaaaccatt gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct
    14761 ctttgatgcg aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca
    14821 ggtatacaaa cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga
    14881 taacaatgtt atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa
    14941 tccgtacatc atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg
    15001 catcggccgg gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt
    15061 tggccggcgc agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg
    15121 tcgcctgaac gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta
    15181 cctatccaac cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa
    15241 acccaaattc cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga
    15301 taatccggct ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc
    15361 tgctcctggg tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga
    15421 acctgcttat tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca
    15481 acaacggtta cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca
    15541 gtcgcgttgt cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga
    15601 cgcaggtcga agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata
    15661 tcttccagca gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg
    15721 ctcgccacgc cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct
    15781 gcaaagtcca cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg
    15841 cctttcagta catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga
    15901 actttcagca gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca
    15961 gtgatcccct gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg
    16021 acgacagcaa cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca
    16081 cctgtaccgg taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc
    16141 gccgcacgca atgcatcgcg atcctcatca gccgcattgc ggccaacttc tggattagcg
    16201 ccagcgccca gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt
    16261 ttacgcagcg cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg
    16321 cgcaccatgt gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc
    16381 gcgtcattgg taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc
    16441 tgaggccgta atcatcgtcg gcctcataaa aattaaaact cttttcgcag ccaactattg
    16501 agtcgcttga tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg
    16561 ttaagatgtg actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc
    16621 tgagcataat ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga
    16681 aacacgcgct gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca
    16741 atgcctgccg ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat
    16801 atctcttcgt tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg
    16861 gtctgacgtt gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca
    16921 tcttttccaa cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg
    16981 cttggcggcg tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga
    17041 attaccttag tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca
    17101 ccgatatcga cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt
    17161 gatgccagtc cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg
    17221 acgatgtttt tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc
    17281 acgcccgaaa gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc
    17341 gggatcacat gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg
    17401 acgttttcca catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca
    17461 ttctggcagc tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct
    17521 gccatcaatt ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg
    17581 ttcaccccgc ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc
    17641 ataccgtcgg gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt
    17701 cctactacca gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt
    17761 gttgctgagt agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat
    17821 aacgcaaatc aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg
    17881 gataaagttc tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct
    17941 taatatcgtt attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca
    18001 gagtaaatct gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat
    18061 tggcgctgcc ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg
    18121 gcacgctgaa ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg
    18181 gcacatattc aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct
    18241 gcttaatcca cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca
    18301 taaaggtacc cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt
    18361 aatggcgttc accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc
    18421 agcccaacac gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga
    18481 tccccgccag acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg
    18541 tgttcagagc agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc
    18601 gagaagctca tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc
    18661 ggtgaggtat tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata
    18721 cgtccccatc ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg
    18781 gcctcttgtg acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac
    18841 ttcgcctcat aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct
    18901 tcaccgagta tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct
    18961 tcatcgtgct gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt
    19021 gacattccca cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca
    19081 gaaatttctg ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct
    19141 acccacggcg cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc
    19201 attgaaagcg cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc
    19261 atgccctgca gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga
    19321 aaacccatcg acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca
    19381 ataccgcctt cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc
    19441 tcagcggaag tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg
    19501 agtttgcggc ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc
    19561 cccctgaacg agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg
    19621 cgcagggtcc ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag
    19681 cgaacggctg tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa
    19741 cagggtatca acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt
    19801 aaaacggtgc ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc
    19861 tttaatggtg gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt
    19921 accgctttta ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc
    19981 agtcccctgg aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt
    20041 agcaaccgca accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt
    20101 gacgcgcatc ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg
    20161 ctgataatct tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg
    20221 tcccacacgc ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc
    20281 gtaaaacggc aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc
    20341 ctggtaggta tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag
    20401 atgcaggaac gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa
    20461 acgcgcatga acccccgccg cttttaccag cccgccgtta acgaaggttg ggtcgagccc
    20521 cgcttctgcg tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc
    20581 aatggcgatg ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac
    20641 cggaatacgc gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga
    20701 aacaacgacc acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc
    20761 tcccagattc attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg
    20821 ataaccttca ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac
    20881 aaaatgtatg tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg
    20941 ttgtgtattc attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc
    21001 tacaattacg cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga
    21061 atggatgcag cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc
    21121 agggtgttgg cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc
    21181 gctttttcca gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca
    21241 aacaacgccg gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa
    21301 acgacgacat ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta
    21361 tgctgcggtt gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg
    21421 ctctgatgcc agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg
    21481 ttaagaatgc gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt
    21541 ccagccaaac gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc
    21601 accacttccg cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc
    21661 gccagccatt tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg
    21721 cctaacgacc acgcggccag accacctgga cctgatacat agccgcccat accgagtacc
    21781 acgtcaggtt tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc
    21841 agcggcgcag ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa
    21901 tcaatttcga tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc
    21961 cagcgaactt gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca
    22021 tgtccaccgg ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac
    22081 ctcgtacaaa cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca
    22141 tgatggctgt cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca
    22201 gacctttggt cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa
    22261 accagatgcc aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt
    22321 tgcggccaat cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca
    22381 caccgacata ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg
    22441 gcagatactc cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac
    22501 caaacgccat cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt
    22561 tccagaatgc ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg
    22621 ctgaaatgcc cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca
    22681 acatcgccag cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca
    22741 gtaacactgc caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac
    22801 gtacttcgtc gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg
    22861 tcagctccgc aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa
    22921 ccgagctacc cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg
    22981 cactgtagcg ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg
    23041 ccaggatcag atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt
    23101 gccctatggg cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg
    23161 tcagccacag taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc
    23221 ccatcaccca gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc
    23281 gcggcatttt caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc
    23341 aaactcattg cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga
    23401 gagcagaacc atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat
    23461 ggtttcggtt tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc
    23521 aaaacagtac aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc
    23581 cgatttacca tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa
    23641 cgccgcttcc gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt
    23701 gtgctccagc accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag
    23761 gctgctggcg cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt
    23821 gtagttatgc tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac
    23881 ccgcagccag gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa
    23941 gctgacgcag cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac
    24001 gacacaaact ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc
    24061 aaacggatag cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg
    24121 taagctggaa gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc
    24181 agcatcaagc agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc
    24241 cgccgctttc gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt
    24301 aatcgccaca atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc
    24361 gattccggca tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc
    24421 gacaatcaga tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac
    24481 ggcttcgggt aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt
    24541 cacaccgcga gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat
    24601 gacgacattt ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca
    24661 atcagaacca gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag
    24721 cctttcagtt catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt
    24781 ttaaaggagc cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata
    24841 atcaccagca ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac
    24901 gccagcgaac ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag
    24961 cccagtcctg ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga
    25021 tacggtatat gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca
    25081 aaaccaccgg cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg
    25141 tttaccgcgt tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc
    25201 agctgcggca tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg
    25261 ccggcaaggt acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc
    25321 caacgagcga tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag
    25381 ccaataacac cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg
    25441 taagcccaca gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc
    25501 ggcgtaccgc gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag
    25561 gaaagttttt gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg
    25621 gtcagcaggc tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa
    25681 tatttgacca aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta
    25741 ctacctcttc catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct
    25801 cagcaatcag tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa
    25861 cgccgctggc ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac
    25921 cagcagcttt tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt
    25981 ccgccatatc gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag
    26041 cggcggtcat tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg
    26101 ccagttggat ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga
    26161 tagcattaag cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa
    26221 tattgtgacg ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg
    26281 taaattccgt accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg
    26341 cattgggtga gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt
    26401 cgttgttgtc ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac
    26461 ctttcgcttt cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt
    26521 tgttgaccag cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat
    26581 ggttcgcgcc aagttcaata actgcgtaat cgtattccgg cgttaagcgc aacaacgtca
    26641 tcggtacacc gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc
    26701 ttaaaatcgc cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa
    26761 ccacgcgcgc cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg
    26821 tatccttgac gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc
    26881 ccgcgccgcc agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca
    26941 gggcaacaaa caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg
    27001 tgatatctgc accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc
    27061 taatcatgca atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg
    27121 ctgattgcca acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt
    27181 ctctttagcc tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc
    27241 atgtccggca tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt
    27301 acgcgggtta tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat
    27361 cagtggacgc ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc
    27421 cgcacagtgc aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta
    27481 atccaccacc accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg
    27541 ttgcagacgc gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt
    27601 cgccagcgcg agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc
    27661 gccatcgccc caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc
    27721 gatcgctttt aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc
    27781 aaccgcgtcc ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat
    27841 cgcctgaccg caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat
    27901 atcaccatga taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt
    27961 caatgccgcc acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt
    28021 cgcgccctgc tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt
    28081 attttctgtc gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct
    28141 ggtttcgcca agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt
    28201 ggtgcccgtt acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc
    28261 cagtgcagat aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc
    28321 acggatttca ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac
    28381 accttgcgct atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc
    28441 tacaaagaga tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg
    28501 cgaaggtgcg tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg
    28561 tcccctcgcc ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct
    28621 cgatgttcat ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg
    28681 cgccgccgta gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg
    28741 gctgactcgc aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt
    28801 ccggcccgac ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg
    28861 ccttcacgcc gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga
    28921 caatggatcc cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca
    28981 gtgggcgata aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg
    29041 ttaccattag cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt
    29101 tttgaggata taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc
    29161 caaaacgtga gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa
    29221 caccgacgtt actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca
    29281 cgtctttgat ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc
    29341 gcaccacgcc acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt
    29401 caaacacgtc ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt
    29461 tagggttgta tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca
    29521 gcacggcgct accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa
    29581 ccagcgcctg caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag
    29641 tagaagaaat atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg
    29701 gctgcccggt aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga
    29761 cgttagtaaa gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact
    29821 cttcacgcag atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt
    29881 tcacctgacg cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg cgggctgaaa
    29941 gctgatccag cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga
    30001 taccgccagc gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca
    30061 ccgctaacgg gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa
    30121 cgcgaagaga acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc
    30181 acgctacgcg tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa
    30241 aacgccaact gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg
    30301 ctgctttcat gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat
    30361 caacatgctg catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga
    30421 gcgcattctc ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca
    30481 ccagttgttc gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca
    30541 ccgccgtcaa aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat
    30601 cgtcaccgat aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt
    30661 tgcttagagc ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac
    30721 tgaactacgg gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag
    30781 tgctcgcagc tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc
    30841 cggaacttgc ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag
    30901 cgagtggaag ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt
    30961 tagcgcctgc tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt
    31021 acgggtcgcg ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc
    31081 cagttctttg gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat
    31141 gcgtttggca aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc
    31201 tgcggtttgt agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag
    31261 cggaccatcg cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac
    31321 gccaagatcg aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc
    31381 gcccagcgcg gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc
    31441 aacggcgata gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg
    31501 cgagaggatc agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc
    31561 aggacggata ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt
    31621 ttccatcatt ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact
    31681 gctctgcgtc gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa
    31741 acttgttgaa ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta
    31801 ctggcgcgat taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca
    31861 gtaggcgctg cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct
    31921 cgataatttc ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg
    31981 tgcaaaccat ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg
    32041 ataagcgccc tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac
    32101 cccttattcc cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa
    32161 agcttgtcaa gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata
    32221 tcagcgttgt ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa
    32281 gaaaaaatcc ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata
    32341 taaagtgtca gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc
    32401 cctgttgcgt ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc
    32461 ttgttaaatc ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat
    32521 atcacttaat tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac
    32581 ttatcaataa tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt
    32641 actctacg
//
LOCUS       DAZPEP010000034        30870 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZPEP010000034 DAZPEP010000000
VERSION     DAZPEP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30870
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.034"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            470..985
                     /locus_tag="ACHUT9_001549"
     CDS             470..985
                     /locus_tag="ACHUT9_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1348055.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            1203..1931
                     /gene="artP"
                     /locus_tag="ACHUT9_001550"
     CDS             1203..1931
                     /gene="artP"
                     /locus_tag="ACHUT9_001550"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFR1348056.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            1949..2680
                     /gene="artI"
                     /locus_tag="ACHUT9_001551"
     CDS             1949..2680
                     /gene="artI"
                     /locus_tag="ACHUT9_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFR1348057.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            2687..3403
                     /gene="artQ"
                     /locus_tag="ACHUT9_001552"
     CDS             2687..3403
                     /gene="artQ"
                     /locus_tag="ACHUT9_001552"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFR1348058.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            3403..4071
                     /gene="artM"
                     /locus_tag="ACHUT9_001553"
     CDS             3403..4071
                     /gene="artM"
                     /locus_tag="ACHUT9_001553"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFR1348059.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            4363..5094
                     /gene="artJ"
                     /locus_tag="ACHUT9_001554"
     CDS             4363..5094
                     /gene="artJ"
                     /locus_tag="ACHUT9_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFR1348060.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(5269..6396)
                     /gene="rlmC"
                     /locus_tag="ACHUT9_001555"
     CDS             complement(5269..6396)
                     /gene="rlmC"
                     /locus_tag="ACHUT9_001555"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFR1348061.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(6437..6925)
                     /locus_tag="ACHUT9_001556"
     CDS             complement(6437..6925)
                     /locus_tag="ACHUT9_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFR1348062.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(6985..7830)
                     /gene="potI"
                     /locus_tag="ACHUT9_001557"
     CDS             complement(6985..7830)
                     /gene="potI"
                     /locus_tag="ACHUT9_001557"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFR1348063.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(7827..8780)
                     /gene="potH"
                     /locus_tag="ACHUT9_001558"
     CDS             complement(7827..8780)
                     /gene="potH"
                     /locus_tag="ACHUT9_001558"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFR1348064.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(8790..9923)
                     /gene="potG"
                     /locus_tag="ACHUT9_001559"
     CDS             complement(8790..9923)
                     /gene="potG"
                     /locus_tag="ACHUT9_001559"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFR1348065.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLNGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(10018..11130)
                     /gene="potF"
                     /locus_tag="ACHUT9_001560"
     CDS             complement(10018..11130)
                     /gene="potF"
                     /locus_tag="ACHUT9_001560"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFR1348066.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(11481..11957)
                     /locus_tag="ACHUT9_001561"
     CDS             complement(11481..11957)
                     /locus_tag="ACHUT9_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFR1348067.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(12045..12947)
                     /gene="rimK"
                     /locus_tag="ACHUT9_001562"
     CDS             complement(12045..12947)
                     /gene="rimK"
                     /locus_tag="ACHUT9_001562"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFR1348068.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(13008..13730)
                     /gene="nfsA"
                     /locus_tag="ACHUT9_001563"
     CDS             complement(13008..13730)
                     /gene="nfsA"
                     /locus_tag="ACHUT9_001563"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFR1348069.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(13714..14001)
                     /locus_tag="ACHUT9_001564"
     CDS             complement(13714..14001)
                     /locus_tag="ACHUT9_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFR1348070.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            14161..14418
                     /gene="grxA"
                     /locus_tag="ACHUT9_001565"
     CDS             14161..14418
                     /gene="grxA"
                     /locus_tag="ACHUT9_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFR1348071.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(14448..14825)
                     /locus_tag="ACHUT9_001566"
     CDS             complement(14448..14825)
                     /locus_tag="ACHUT9_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFR1348072.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            15095..16780
                     /locus_tag="ACHUT9_001567"
     CDS             15095..16780
                     /locus_tag="ACHUT9_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFR1348073.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(16955..17491)
                     /gene="rcdA"
                     /locus_tag="ACHUT9_001568"
     CDS             complement(16955..17491)
                     /gene="rcdA"
                     /locus_tag="ACHUT9_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFR1348074.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            17575..18783
                     /locus_tag="ACHUT9_001569"
     CDS             17575..18783
                     /locus_tag="ACHUT9_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1348075.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            18783..19598
                     /gene="ybjI"
                     /locus_tag="ACHUT9_001570"
     CDS             18783..19598
                     /gene="ybjI"
                     /locus_tag="ACHUT9_001570"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFR1348076.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            19684..19968
                     /gene="ybjH"
                     /locus_tag="ACHUT9_001571"
     CDS             19684..19968
                     /gene="ybjH"
                     /locus_tag="ACHUT9_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFR1348077.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTGDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(20009..21236)
                     /gene="mdfA"
                     /locus_tag="ACHUT9_001572"
                     /pseudo
     CDS             complement(20009..21236)
                     /gene="mdfA"
                     /locus_tag="ACHUT9_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            21521..22117
                     /gene="ybjG"
                     /locus_tag="ACHUT9_001573"
     CDS             21521..22117
                     /gene="ybjG"
                     /locus_tag="ACHUT9_001573"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1348078.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            22175..22933
                     /gene="deoR"
                     /locus_tag="ACHUT9_001574"
     CDS             22175..22933
                     /gene="deoR"
                     /locus_tag="ACHUT9_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFR1348079.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(22980..24182)
                     /gene="dacC"
                     /locus_tag="ACHUT9_001575"
     CDS             complement(22980..24182)
                     /gene="dacC"
                     /locus_tag="ACHUT9_001575"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFR1348080.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            24429..25054
                     /gene="gstB"
                     /locus_tag="ACHUT9_001576"
                     /pseudo
     CDS             24429..25054
                     /gene="gstB"
                     /locus_tag="ACHUT9_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(25051..26166)
                     /gene="yliI"
                     /locus_tag="ACHUT9_001577"
     CDS             complement(25051..26166)
                     /gene="yliI"
                     /locus_tag="ACHUT9_001577"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFR1348081.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(26277..26660)
                     /gene="bssR"
                     /locus_tag="ACHUT9_001578"
     CDS             complement(26277..26660)
                     /gene="bssR"
                     /locus_tag="ACHUT9_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFR1348082.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            26873..28198
                     /gene="rimO"
                     /locus_tag="ACHUT9_001579"
     CDS             26873..28198
                     /gene="rimO"
                     /locus_tag="ACHUT9_001579"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFR1348083.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(28245..29566)
                     /locus_tag="ACHUT9_001580"
                     /pseudo
     CDS             complement(28245..29566)
                     /locus_tag="ACHUT9_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(29574..>30870)
                     /locus_tag="ACHUT9_001581"
     CDS             complement(29574..>30870)
                     /locus_tag="ACHUT9_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
                     /protein_id="HFR1348084.1"
                     /translation="SIAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLA
                     VGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENV
                     QTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFS
                     EFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVS
                     AERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPW
                     QLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISID
                     DFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVE
                     SEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         7780 a         7962 c         7480 g         7648 t
ORIGIN      
        1 tcgagagtca aagctgggac tatcaaattt cgctcatttt caaaaataca acgtaatttt
       61 ggcaatcatc ttaatcgcag gatctcttat cgcatgtgaa aaattttgta atgattagac
      121 aataaccgcg ttcatttatc acgcgttata aatgtgaaac tggcttcttg cagatgctct
      181 taacctctac aggaggattt ataccaccat ttcagaacta tcatatttac cttaaatgat
      241 catttcagcc atataacacg ctcaacctca caaatggttt acactgtcta ttgtttgggt
      301 aatttaagta agtcagctac gagcaacctt gagtggatag ccagcaatgg actcaaaccc
      361 aatcaggtgt tcagattgtt tcagaattgt attaaaaaca atgtattaaa taatctgata
      421 gcgtttggtc gtagtgtgac gcagacagca agctaataag gaaatgatta tgcgctaccg
      481 ttcactatct ctacttattc catgtgcact gctgctgagt gcctgtacca ccgtcacgcc
      541 agcttataaa gataacggtc cccgcaccgg ttcctgtgtc cagggggggc ctgacagcgt
      601 ggctcaacaa ttctatgact accgcattca acatcgcagc aatgacatta ccgccctgcg
      661 cccctatttg agtgacaaac tggcaaccct tcttagcgat gccagccgcg ataatagtca
      721 tcgtgaattg ttaagcagcg atccgttctc cagtcgaacc accttgccag atagcgccca
      781 cgttgccagt gcatcgacta tccctaatcg tgatgcccgt aacattccat tgcgcgtaga
      841 tctcaaacag ggtgaccagg gttggcagga tgaagtgtta atgattcagg aaggccagtg
      901 ttgggtcatc gacgatgtgc gctacttagg cggcagcgtt catgccacgg cgggtacgct
      961 ccgtcagtcc atcgagaatc gctaacgcct tgatcaacca ataaaccccg taaattgtcc
     1021 ggcatttcgt gaggaatgcc gacatttatg ctcgccgacc accgcccccg ttattttgtg
     1081 ctatgtttat tgaataatgc gctttgcttt taacttttaa agcagaaata ttgcataatt
     1141 attctgtcaa aggtactatc ttcgacctca atagctatca gactgccagt atacgagtgt
     1201 caatgagtat tcaattaaac ggcattaatt gcttctacgg cgcgcatcag gcgctgttcg
     1261 atatcacgct ggattgccca cagggcgaaa cgctggtgtt acttggcccc agcggcgcgg
     1321 gtaaaagctc gctgctgcgt gtactcaatc tgcttgagat gccacgctcc ggtacgctca
     1381 acattgcagg caaccatttc gatttcacca aaacaccctc tgacaaagcg attcgcgatt
     1441 tgcgtcgtaa cgttggcatg gtgtttcagc aatacaacct gtggccgcat ctgaccgtgc
     1501 agcaaaacct gattgaagcg ccctgccgtg tactggggtt gagtaaagat caggcgctgg
     1561 cccgtgcaga aaaactgctg gaacgtctgc gtctcaaacc ttatagcgat cgttacccgc
     1621 tgcatctttc tggtggtcag cagcagcgtg ttgctattgc ccgtgcgttg atgatggaac
     1681 cgcaggtact gctgttcgat gaaccgaccg ccgcactgga cccggaaatt acggcacaaa
     1741 tcgtcagcat cattcgtgag ctggcagaaa cgaatattac ccaggtgatc gtcacccacg
     1801 aagttgaagt ggcgcgtaaa accgccagcc gagtggtgta tatggaaaat ggtcatatcg
     1861 tagaacaagg cgacgcgagc tgctttaccg agccgcaaac cgaagcattt aaaaactatc
     1921 tctctcacta agattcggga taacaacaat gaaaaaagtt ctgattgccg cgttaattgc
     1981 aggttttagt ctttccgcca cagctgccga aaccattcgt tttgctaccg aagcatccta
     2041 tcctccgttt gaatcgattg atgcaaacaa ccagatcgtt ggttttgacg tcgacctggc
     2101 acaagcgctg tgtaaagaga ttgatgcaac ctgcactttc tctaaccagg cgtttgacag
     2161 cctgatccca agcctgaaat tccgtcgcgt agaagccgtg atggcgggca tggatatcac
     2221 tccggagcgt gaaaagcagg tgctgtttac caccccgtac tatgacaact ctgccctgtt
     2281 tgtgggtcag caaggcaaat acaccagtgt tgatcagctg aaaggcaaaa aagtcggcgt
     2341 acagaacggg acgacacacc agaaattcat tatggataag cacccggaaa tcactaccgt
     2401 tccgtatgac agctaccaga acgcaaaact ggatctgcaa aacgggcgta tcgacggcgt
     2461 cttcggtgac accgcagtgg tcactgagtg gctgaaagat aacccgaaac tggcggcggt
     2521 gggcgacaaa gtgaccgata aagattactt cggcactggc ctcggcatcg cggtacgtca
     2581 gggcaacact gagctgcagc agaaactcaa cactgcgctg gaaaaagtga agaaagatgg
     2641 cacttacgaa accatctaca acaaatggtt ccagaagtaa ttcctgatga atgaattttt
     2701 tcctttagca agcgccgccg ggatgaccgt cggccttgcc gtttgtgcat tgattgtcgg
     2761 gctggcgctg gcgatgttct ttgcggtatg ggagtcggca aaatggcgtc ctgtcgcgtg
     2821 ggcaggttca gcgctggtaa ccattctgcg tggcctgcca gaaattctgg tggtgctgtt
     2881 tatctatttt ggctcctcgc agctgctgct gacgctttcg gatggcttca ctatcaatct
     2941 tgggttcgtg cagatcccag tgcagatgga cattgagaac ttcgacgtta gcccgttcct
     3001 ttgtggtgtc atcgctctgt cactgctgta tgccgcctat gcctcgcaaa cgcttcgggg
     3061 cgcgttgaaa gcggtgccgg tgggtcagtg ggagtctggt caggcgctgg ggctgtcgaa
     3121 atcggctatc tttttccgtc tggtgatgcc gcagatgtgg cgtcatgcgc tgcctggcct
     3181 cggtaaccag tggctggtgc tgctgaaaga taccgcgctg gtcagtttga ttagtgtgaa
     3241 tgatttaatg ctgcaaacaa aaagcatcgc tactcgtacc caggaaccat ttacctggta
     3301 cattgtggcg gcggcgattt acctggtgat caccctgctc agtcagtaca ttctcaaacg
     3361 cattgacctg cgcgcgacac gttttgagcg gaggcccagc taatgtttga gtatttaccc
     3421 gaactgatga aagggctaca caccagcctg acgctaaccg ttgcctcgct gattgtggca
     3481 ctgattctgg cattgatttt taccatcatc ctgacgctga aaacgccggt gctggtgtgg
     3541 ctggtgcggg gttatatcac gctgtttacc ggtacgccgc tgctggtgca gatcttcctg
     3601 atttattacg ggccgggcca gtttccgacg ttgcaggagt atccggcact gtggcatttg
     3661 ttgtcagaac cgtggttatg tgcgctgatt gcgttatcgc tgaacagtgc ggcgtatacc
     3721 acgcagctgt tttacggtgc aattcgtgcg atcccagaag gtcagtggca gtcctgtagc
     3781 gccctgggaa tgagcaaaaa agatacgctg gcgctcctgc tgccgtatgc ctttaaacgc
     3841 tcgctctctt cttattccaa cgaagtggtg ctggtgttca aaagtacctc tctggcttac
     3901 accattacgc tgatggaagt gatgggatac agccagttgt tgtacggacg cacctacgat
     3961 gtaatggtgt tcggtgcggc agggattatt tacctggtcg tcaacggcct gctgacgctg
     4021 atgatgcgtc tgatcgagcg caaagcactg gcattcgaac ggcggaatta atgtagctgc
     4081 gtacattcgc cgggggggtt tgtaggtctg ataagacgcg ccagcgtcgc atcagacatc
     4141 agcacggtgc ctgtgccgga tgcggcgtgg acgccttatc cggcctacat agtgcataaa
     4201 ttcttataat gaagacggac aactcactaa gttatccgtc tttttttatt tcatttaaat
     4261 tatttaatca tatttattgc atataaattc acttgatagc attgttatcc catgccgcag
     4321 acacggccaa aaatcataag attgacagac gggagttcca tcatgaaaaa gttagttctt
     4381 gccgctttac ttgcttcctt tactttcggt gcttctgccg cagagaaaat caattttggc
     4441 gtttcagcca cctatccacc ctttgaatct ataggtgcta ataatgagat tgtcggcttt
     4501 gatatcgatc tggcaaaagc cttgtgcaaa caaatgcagg cagaatgtac ttttactaat
     4561 cacgcgttcg acagcctgat cccgtcactg aaattcagaa aatatgactc cgtaatctcc
     4621 ggtatggata tcacgcccga gcgtagcaag caggtatcgt ttaccacgcc ctactatgaa
     4681 aactcagccg tcgtgattgc caaaaaagat acctacaaaa cgtttgccga tctgaaaggc
     4741 aaacgtattg ggatggaaaa cggcaccacg caccagaaat atattcagga tcagcacccc
     4801 gaagtgaaaa ctgtctccta tgacagttat cagaatgcct ttatcgatct gaaaaatggt
     4861 cgtattgatg gggtatttgg tgacacagcg gtggtaaacg aatggctgaa aaccaatccg
     4921 caactgggcg ttgctactga aaaagtgacc gatccgcaat acttcggcac cggtctgggc
     4981 atcgctgtac gtccggataa caaagctctg ctggaaaaac tgaataacgc gctggcagca
     5041 attaaagctg acggtacata tcagaaaatc aatgaccagt ggttcccgca gtaattgaga
     5101 ctgatgacaa aagtaagatt gcctgatgcg ctgcgcttat ctggcccaca tcaactctgc
     5161 aatgtattga tttagcatga ttttgtaggc cggataaggc gtacacgccg catccggcat
     5221 gaacaaagcg cacctgatca gcaatctgaa gccgcacctg cggctttttt attgcttcac
     5281 cagcagcgtc atcacttcat agtgcgcggt gtgcgggaac atatcaaaaa gctgtacccg
     5341 ttcgatgcgg taacctggca gttcgcggat atctttcgcc atggtttggg cgttacagct
     5401 ggagtagata ataaaacgcg gtgccatcgt tgagagataa tcacacagcg gtttaccaat
     5461 gccgcggcgc ggcgggttaa ccagcaccag ctccggcaca tccccctgag cggtggcaaa
     5521 ctgagtggag tccagcgcct gaaattgcaa acgcgttaag cccagttcag cggctgactg
     5581 ctttgcacag gcaatggcct ctgatgcaat ttcgatcccg gttaactgca tgtcaggcgt
     5641 cgcgcagtgt aaaccaaagc cacccacacc gcagaacaga tcccacatat gtttaactgg
     5701 cagctgccgt acccagtcgc gcgcggtagc gtacagctgg ctggcgaccg ccggattagt
     5761 ctggaagaaa ctttgcggac ggatccacag cggtacgtca ttaaaacgct ctgccagcgc
     5821 ctgttgttca gtcaggtaga tctccgtctc cccttccata atcgccatat gtaccggctg
     5881 aatattgacg gtaataactt tcagttgcgg tagttgttcc tgtaaccacg gcagcgcctt
     5941 acgcagttgt gccagtttgg tttcagaacg cagtacaaag cgcagcatca tgccgccatc
     6001 gctctggctt tcagtcagca gaatgtattt cagttcgcca cgcttacgcg ccacgttgta
     6061 gggcgttaac cccgcacggg cgataaacgg ttttagcgcc gcaaaaacgg gcgcaaaaga
     6121 ggcaggataa agcgggcagt cacaaaggtc ttccggcgtg ccatcgcgat gcagcatacc
     6181 gagcagtggt ttttcaacgc taccactcac caccattttg gctttattac gaaacccttg
     6241 ttccgggcct gacaccggcg cgcaccattc ctcaaccgga aaatcggcga gcagattttt
     6301 aagatcggcg gttttagcgg agagttgctc tggaatcggc tgcgttatcc actgacagga
     6361 acgacagcga cccgcgtcgt aaagtgcgca ctgcatacat tgaccttcac atcatcaggg
     6421 gcgacgatta tacacattat tgcaactgga agaaccgccg actggtcgag ggaacgaaca
     6481 gcagcatcag gatgagcata tccggcagct tctgcagcat caggctatgg aagatttcac
     6541 gttttgattc accgggaatg ctgaacagct ccggataacc gtaccccagc gaagccgccc
     6601 acaagtaact tgcggcggtg atttgtgtca gcagatatag ccagcgcgcc cagcgacgcc
     6661 ctttcaccag tgaaaacgca caccagatct caatgaaaac cagcaccaga ctgcttaaaa
     6721 agaccagcgt taaactccag gtttgtacgc tgcgatgaat gaactcgcca ataccgcgca
     6781 cgcccagcgt attgaaaatc atcagcacgt cgaggccacg gatcataata atggcgagcg
     6841 ccgccacctg caccagcgca ggcacattca ggcgagcatg agatgaagat gttttcttaa
     6901 aaaatcccaa cgtttcgtct tccatgaaaa caatgccgcg gcatgcgcgg cattatgtag
     6961 ccaggttggc aaattttagt gtcttcagcc atgtcttgca cgctggatat cgcgtatccg
     7021 ctgtttttct gcgcgagcca tcagatacca ggcgataaat ccgacaattc cgaccgcacc
     7081 gagaattaac gttgcaaggg cgttgatttc cggattcact cccatccgca cgctggaaaa
     7141 gaccagcatc ggtaacgtgg tggctcccgg cccggaaaca aagctggcga tcaccagatc
     7201 atcaagcgac aaagtaaaag ccagtaacca gccagaaatg atcgcgggca tgatcatcgg
     7261 tagcgtaatg acgaaaaaca ctttcagcgg cgtcgcaccg agatccatcg ctgcttcttc
     7321 tatcgagcga tccagttccc gcaatcttga agaaataacg accgccacat aagccgtaca
     7381 aaaagtgaca tgcgccagcc agatggtgag cataccgcgg tccgcaggcc agccaatggc
     7441 atgagcaagc gcgacgaata ataacaacag cgacaagccc gtgatgacat ctggcatcac
     7501 cagcggcgcg gtgatcataa aggcaaaacc atttgatccg cgaaacctgc caaaacgcac
     7561 cagcaccacc gccgcaatag tcccgaggat cgccgccgcc gttgccgcac aggcagcaat
     7621 tgtcaggctt aaaccaaccg cactcatcat cgcgtcatcg cgcaataact caccatacca
     7681 gcgcgttgac cagccggccc acaccgtcac cagcttcgag ctgttaaacg aatagataac
     7741 cagcatcagc attggcgcgt agagaaaggt aaagcccagc agcaaaatca caatccgcca
     7801 gggcgaacga actaccggta aattattcat ccgtgttctc ccacgctttt ttgctggtgt
     7861 ttgtggaacc acattatcgg cacaattagc agcagcaaca tgatgatcgc taccgccgag
     7921 gccaccggcc agtcgcggtt attgaaaaac tcttgccata acacgcgccc gatcatgatg
     7981 ctgtccgggc caccgagcag ttccgggatc acaaactcgc ccacagccgg gataaacacc
     8041 agcatcgatc cggcaataat cccaccttta gtcagcggca cgatcacagt aaagaacgtt
     8101 ttcagcggtc gtgcaccgag atccagcgct gcttccacca gcgaataatc aatacgaatc
     8161 aacgcggtat aaatcggcag taccataaac ggcacgtaag cgtaaacaat gccgatataa
     8221 acggccagat tggtatgcag aatggtcagc ggttgatcga taacccccag ccacagcaga
     8281 aaattattca gcacaccgtt gttttttaat attcccatcc aggcataaac gcggatcaga
     8341 aacgaggtcc acgacggcag gatcaccagt agtaataaaa tattacgggt cgaaggctta
     8401 ctgtgcgcca ccgcccacgc cagcggatag ccgatcagta aacagcaaaa tgtcgaaatc
     8461 gccgccacct gtaacgactg gagataagca tcaaaataga gcggatcgtc ggtcagttgc
     8521 agaaaattac cgagattaag agtgatggaa agttgcccgt cagcccactc catcagttcg
     8581 gtataaggtg gaatagcgcg cgccatctct cccaggctta ttttaaagac aatcagaaat
     8641 ggcagcagaa acagcaagat caaccagata tatagcaacg caatgaccag tttgcgccca
     8701 tgcttcattt gcagctgcga caaccacagc ttaaatccgc ccggcggttt cgactgggca
     8761 gcaggttcaa gtgtactcat ctttgctcct taaaccgtca gcaccacaca gctgtccact
     8821 tcccagcaca aacgcacttc gtcgccccag gtcggtaacc ctttacgatg acgatgggcg
     8881 ttttgtagct gggcgctgat catctgccca cttttcagtc gaacgtgata caccgaaaga
     8941 tcgccgagat aggcaatgtg tatcacctcc cccaccgcga agttacaacc attggcgggc
     9001 ggctcttcgc aaagcatgat tttttccggg cgcagcgcta cgtgtaccgg cacgttatcg
     9061 accactgaag catccgcatc gactttcagt ggatgcacca gccccggcga atcaagcacc
     9121 aggccatctt cctgacgctc tttgagtaca ccttcaaaga cattaaccga accgataaat
     9181 tcagcgctgt agcgggtagt tggatgctcg tagatctctt ccggctcgcc aatctggaca
     9241 aatttcccgc gattcataat ggcgatacgc cccgccatgg tcatcgcctc ttcctgatcg
     9301 tgggtgacca tcacacaagt cacaccgacg cgctccagaa tatccaccac ttcaagctgc
     9361 atccggtcac gcagcttttt atccagcgcg cccatcggct catcgagcag taatagtttc
     9421 gggcgtttgg caaggcttcg ggccagggcc acacgctgtc gctgaccacc ggaaagctga
     9481 tgcggtttgc gtttggcgaa ctcctgcatg tgcaccaggc cgagcatctc atggacccga
     9541 ctggcaattt ccgctttcgg tagtttgtcc tgtttcaggc caaaagcgat gttctgttcc
     9601 acggtcatat gcggaaacag cgcgtaagac tgaaacatca tattgatggg gcgcaggtaa
     9661 ggcggcacct gtgacaaatc gacgccattg agcattatct gcccggcaga aggttgttca
     9721 aaacctgcca gcatacgcag cagcgtggac ttgccacagc cggatgcgcc cagcagcgcg
     9781 aagatttcac ctttgtagat ggtcaggctg acatcatcga ccgcatgctg accatcgtag
     9841 gatttggtga ggttgcggat ttctaatagc ggcgtcagcg ccttacgggt tttcgcctgc
     9901 gggcgaggga tagcgtcatt cacggctgct ctccggcatt gaatgtgtgc aagcgcacca
     9961 tgatggtgcg aattgccggc tggtgtggtg cgcccgcccg gcatctaagg ctgcggatta
    10021 ttttccgctc ttcactttgg tccacgcgcg ggtgcgcaca cggtcgattt tcggatcctg
    10081 tactttcaga gtgaatagct tcgcacgaac gtccgccgga ggataaatac ccgggttctc
    10141 acggacttcc gcactcacca gcggcgtggc tgctttattg gcgttggcat agaacacatg
    10201 gtcggaaata tgcgctacta catccgggcg cagcaggtaa ttcaggaact gataggcttc
    10261 gtctttgttt ttggcatccg caggcatggc gaatacatca aagaacgcca tcgccccttc
    10321 ttttggaatc gagaacgaga cattcactcc attcttcgct tccttcgcgc ggtttgacgc
    10381 ctgccagaca tcacctgccc agccgatagc gacgcaaata tcgccgtttg ccaggtcgtt
    10441 aatgtattga gatgaatgga aataacgaat gttcgggcgc agctttaaca gcagatcggt
    10501 tgccggtccg gtgtaatcat ccgctttagt gctgttggga tctttgccga gataattcaa
    10561 cacggtagca aaaacttctt ctggcgcatc caggaaagag acaccgcagc ttttcagttt
    10621 ttccagattt tcaggtttga ggatcaagtc ccagctatcg acgggcgcgt tttcgcccag
    10681 cacagcttta actttatcaa cgttatagcc aatcccggtc gtcgcccaca tatagggcat
    10741 agcaaattta ttgtcgggat cgtgtttggc gaccagcttc agcagctccg ggtcgagatt
    10801 cttccactcc ggcaatttgc ttttgtccag cggctggaaa actcccgcag tcaactggcg
    10861 ttcaagaaag ctggcagatg gaaccaccaa gtcaaagccg gtgctacccg ccattaattt
    10921 gccttccagt acttcgttag agtcgaaaac atcgtagacg actttaatac cggtttcttt
    10981 ttcaaaattg gccaccgtgt ccggggcgat ataatcagac cagttataaa tgtggagtgt
    11041 tttttgttca gccgcgagcg tgccgacaga gacggccatc agagcacccg cagccagacc
    11101 cgaaagccat tttttattta aggcggtcat atctctttcc ttctgaaagt tcgttaacaa
    11161 acgaacaaaa gttgtcacac taaaagatat gcaataaacg tgcatatttt ttatcggcgg
    11221 ggtgtaagaa aagagaactc tctcccggcc agcactgctc gctatataaa agcatcgcaa
    11281 gaataacttt agccagggtt tgagactata gctcggatta aaaaacggta tttaacaaat
    11341 ttttatgcgt atttgctcta tgtgataaag ccaaatcgac acaagagaag tgaaataatc
    11401 tttaagaaaa ggttaaaaac agcagataat tttgcagcgc gcagccactg caccagcgac
    11461 tgcgtgctcg aaaggagtta tcagtggagg aaatagtttt cggtaacgtt gttttgccct
    11521 tcatcgtcag tcggtaacag cagttgatgg gcattagctt caagaatgac catcgaaatc
    11581 tgctcttcgc tctgacgcac gaaccatgca aactgctcat aggttacgcc ctgcataacg
    11641 gataaagact ggcaaaccac cagctttggc agattatcat cctgcatatc aagaaatgct
    11701 ttcacggtca gcgaactggc attgatggca gataaatccg ccgccagcgg caatacggct
    11761 gaaggtcgga cttccgccat ggcagaaaac aggatcgtgt tatcgatcag atcgattttg
    11821 gcatcaaaga caccgtcgaa attctgcata tggggcagat gcagagcctg acagttatca
    11881 cattcaaaaa aactcatccc caggtcatcg agccattgac gcagcgtatc cagaccagga
    11941 acgaccagcg atgtcataat tttgtacaac ctctttcgat aaaaagaccg gcacagctta
    12001 cgcaaaaagc gcaggcaaaa ccatgatcag taatgtgatt gcgactaacc acccgttttc
    12061 aggcaatatt ctgtcgtagc gtggcgttcg atccagcgga tcattttacc cgcgatgtcg
    12121 ataccagtgg ttttttctat tccttccagc cccggcgacg cattcacttc catcaccaac
    12181 ggcccgcgat tagcacgcag aatatccaca ccagcaacgt ccagcgccat cgttcgcgcg
    12241 gcttttatcg cgatttcacg ctcctgtggt gtgatactgg cgacgcttgc cgcgccgcca
    12301 cgatgcaaat tggaacgaaa atcgccctct ttcgcccgcc gttcaatcgc agcgaccact
    12361 tcatcgccaa caaccagaca gcggatatcg cacccttgcg cctctttgat atattcctgc
    12421 accagaatat gcgcgttcag accgcggaaa gcgtcaatca cgctttccgc cgcctgacgc
    12481 gtctccgcca gcacgacgcc aattccctgc gtgccttcaa ccaacttgac caccagcggc
    12541 gcaccaccga ccatgtcgat taaatcgctg gtatcatccg gcgaatgcgc aatgcccgtg
    12601 acaggcaggt cgatgccctg acgcgccagc agttgcatgg aacgcaattt gtcacgcgcc
    12661 cgggcaatgg cgaccgactc attgagcgga tagctcccca gcatctcgaa ctggcgcagt
    12721 gccgccgtcc cataaaaggt aatggcggtg ccaatacgcg ggatcactgc gtcaaaatgg
    12781 ggtaacttgc ggcctttgta gtgaatagaa gacgccgcag gatttatgtt catgtagcaa
    12841 gaaagcggat caagaatttc aaccaggtga ccgcgctgta tagcggcttc acgcagccgc
    12901 ttacacgaat agagcgttcc atcccgggac aatatggcaa ttttcaccct gcacctctct
    12961 gtcagacctg gtcaaaagcc cgcgtatcat acatcgacgt ggcggtttta gcgcgtcgcc
    13021 caaccctgtt tgtgcaaata atccagaata aatgggcggc tttctttaat gattgttcgg
    13081 cggatatgat cgctccaggt atcccggcga ttattgctgc cacgggtgag gtaatattcc
    13141 gccagttgct cgtcatactg cgccagtgcg tctttatcca gcggttgata gctgttttca
    13201 tgcaccaaaa tggaggccgg taaacgcggc ttgagatccg gattatccgc aggccagcca
    13261 aggcacagcc caaacagcgg cagaacatgc tgcggtaatt tcagcagttt cgtcaccgct
    13321 tcaatattat tgcgcaggcc gccgatatat accccgccca atcccagcga ttccgctgcg
    13381 gttaatgcat tctgcgccat cattgccgta tcaacgacac cgagcaacag ttgttccgcc
    13441 aggccgagct gagcatccgg acagatctgt aaatggcggt taaagtcggc acagaacacc
    13501 cagaactccg ccgcttgcgc tacgtgtttt tgcccgccgg tcagcgtcac cagttcttca
    13561 cgtaatgctt tgtcggtaat gcgaataatg ctactacact gcaaaaaact ggaactggac
    13621 gtcgcacggg cgctgttaat aatcgcctca cactgcgctt cggaaatggg ttcatcagtg
    13681 aaatggcgaa tggagcgatg gccacaaata agttcaatgg ttggcgtcat tatctttttc
    13741 tctttctgaa cgtgaatatt gcggtggacg gttcatcagc tgtggggcaa gacgttttgc
    13801 cacctgaaga ataaccacca ccgcagcggg aagcatgagc aaaacaccga gaaaaatcat
    13861 cagaatctgc acttctggcc gagaaaatgg ctcaggcagc gacagggagt cgcttaccga
    13921 cagcagcgcc accgccagta gcatcattcc gataaattcc agtatcaaca cgcctttagg
    13981 caatttaccg atcgcgcgca tacgcttccc tctgcaaagt gagccttcag tctaaaactt
    14041 ttcactgtat tgtgtttaac agttatagct tttagcaatt aatgcaacag gttaaaccta
    14101 ctttcagcga atacatttta gcgtgatcat tacaggcata aatctatgag gagagaaata
    14161 atgcaaaccg ttatttttgg tcgttcgggt tgcccttact gtgtgcgtgc aaaagatctg
    14221 gctgagaaat tgagcaatga acgcgatgat tttcagtatc agtatgtaga tattcgtgcg
    14281 gaagggatca ctaaagaaga tctacaacaa aaggcaggta aacccgtaga aaccgtgccg
    14341 cagatttttg tcgatcagca acatatcggc ggctataccg attttgctgc atgggtgaaa
    14401 gaaaatctgg acgcctgatc gtctgacaag ccctcgcgtt gagggcttta ctgatttttt
    14461 ctgtgctgtg gtttaaacaa actactgata aataagaaac acagtgcccc cagcgcacac
    14521 cagaacaccg cgcttagtaa ccatgccagc tcttgccaga atgagcgcgt cggtgaaaaa
    14581 aacagccgca taatgagcat tgaacagggt gccgccagca ttgcgccaaa tagaggtttc
    14641 aggacttctc tacgctgtga aaagaaactg gcaactgctc caggaagaat gaaaaacagc
    14701 aagccgattt caggatgccc ggcagcccga aaagcgcctt tcatgtgcgt cgccagaaaa
    14761 aggcacacca caatgaagag gacaaaacag cagattgccc ccgcccaacg ttgtttatgt
    14821 ttcactcgtt cctcctgaca ctgcgtctat cgaacacatt tttcgccagt gtggcgttca
    14881 gtaagataaa gccgcttcgc attccatgct aatataggcc aacgcaattc atatagccgt
    14941 tgatacctaa tgtgattaca ctagtaaaat atattgttac tttactatcg tttaggtgcg
    15001 ctgaatgaat ctgcgccctg aattctggta aaaaacatta tcgtaaatta ccatttcttt
    15061 caacagctta ctagtaaaca agaagttagc ctccgtgaat ataaacgtcg ccgaattgtt
    15121 aaatgggaat tacattctgt tattatttgt ggtcctcgcg cttgggctat gtctcggaaa
    15181 gttacgactt ggttcgatcc aactgggtaa ttccattggc gttttagtcg tatcgctgtt
    15241 attaggccaa caacatttca gcattaacac cgatgcgctt aatcttggct ttatgctgtt
    15301 tattttctgc gtcggggtcg aagccggacc gaactttttt tccatttttt ttcgcgatgg
    15361 gaaaaattac ctaatgttag cactggtgat ggttggcagt gcgctggtga tcgccttagg
    15421 gttaggtaag ctgtttggct gggatattgg cctgacggcc ggtatgttag caggctctat
    15481 gacgtcgaca ccggttctgg tcggtgctgg cgatacactg cgtcattccg gcatggaaag
    15541 caggcagctc tcactggcac tggataatct gagcctcggg tatgccttaa cctatttaat
    15601 cggtctggtg agtttgattg ttggtgcgcg ttacttgccg aaattgcagc atcaggactt
    15661 acagaccagc gcccagcaaa tcgcccgcga acgtggcctg gacactgatg ccaaccgtaa
    15721 ggtttattta ccggtgatcc gcgcctatcg cgtcggcccg gagctggtgg cctggaccga
    15781 cggcaaaaat ctgcgtgaac tgggtattta tcgacaaacc ggctgctaca ttgaacgtat
    15841 tcgacgtaac gggattctgg caaatccaga cggtgatgcc gtgctacaaa tgggcgatga
    15901 aatagcgttg gtaggctatc ccgacgccca tgcccgactc gatcccagct tccgtaacgg
    15961 taaagaagtt ttcgatcgtg accttctcga catgcgtatc gtcactgaag aagtggtcgt
    16021 taaaaaccat aacgctgtag gtaaacgtct cgcacaactg aagttgaccg atcacggttg
    16081 cttccttaac cgcgtcattc gtagccagat tgagatgccg atagatgaca acgtcgtgct
    16141 taacaaaggt gacgttttac aagtcagcgg tgatgcccgt cgcgtaaaaa ccatcgccga
    16201 tcgcatcggc tttatctcga ttcacagcca ggtcactgac ctgctggcat tttgcgcctt
    16261 ctttgttatt gggctgatga tcgggatgat caccttccag ttcagcacat tcagtttcgg
    16321 catggggaac gctgccgggt tgttattcgc cggaattatg ctgggcttta tgcgtgctaa
    16381 ccacccgacc ttcggttaca ttccgcaagg tgcattaagc atggtgaaag agttcggctt
    16441 gatggtgttt atggcaggcg ttggtctgag cgccggtagc ggtattaata acggcctggg
    16501 cgcgattggc ggtcagatgt tgattgccgg attaattgtc agtcttgtgc ccgtggttat
    16561 ctgtttcttg ttcggtgctt atgtattgcg aatgaaccgc gcactgttgt tcggcgcaat
    16621 gatgggcgca cgcacctgcg cgccggcaat ggagatcatc agtgatacag ctcgcagtaa
    16681 catccctgcg ctgggctatg cgggcaccta cgcaatcgcc aacgtcctgc tgacgctggc
    16741 agggacaatc atcgtcatgg tatggccagg attaggataa aactgaagtt gccctgaaaa
    16801 tgaaattttt ttgcacaacc gcagaacttt tccgcagggc atcagtctta attagtgcca
    16861 ctgcttttct ttgatgtccc cattttgtgg agcccatcaa ccccgccatt tcggttcaag
    16921 gttgatgggt tttttgttat ctaaaattta tctactaccc tgcaaccctc tcaaccatcc
    16981 tcaaaatctc ctcgcgcgat agcggcttac ggtcggtgac aaaatgcagc gtcatcccct
    17041 caataaacgc atcaagcgcg cgggcggttc cgggttcaaa ccattgttcg agcgtttgct
    17101 gactgcgctg catccagttt tgcattaccg tttttaatag cggttttcgg ctagccagcg
    17161 cgtagagctg gtacatcagc tccatattat ccggcgttgc aacctgtgag ctgtagatca
    17221 tatcggtgat agcctggcat gcgccttgag catcactaac atcgctaaaa aatgcctgat
    17281 attgccggga cataatctca gtaaaactgc tgaacgcctc cagcaacaac tcatcaattc
    17341 ctgaaaaata gtaggtcatc gatcccaacg gtaccccggc aagggtagca attttgcggt
    17401 gcgtaacagc atgtattccg taaagtttca ccgcctccag cgtggcctgg ataatttttt
    17461 ctcgccgttg cggatcgtta gcgcgacgca tgtcatttcc tctgtctgca attgtgtaca
    17521 aatgtacaca accttgctaa tgttgtcttc cctcttctta ttttgacgtg atctatgacc
    17581 gtaaattctt cacgtaatgc attgaaacgc cgaacctggg cgctgtttat gttcttcttt
    17641 ttgccaggcc tgttaatggc gtcctgggca acccgtacgc ctgctatccg cgatattctc
    17701 tctgtctcga tcgctgaaat gggcggtgtt ctctttggtc tgtcgatcgg ttcgatgagc
    17761 ggtattctct gctcggcgtg gttagtgaaa cgctttggga cacgtaatgt catcctggtc
    17821 acgatgtcct gcgcattgat cgggatgatg atattaagtc tggcactctg gctgacatcg
    17881 cccctgctct ttgccgttgg tctcggcgtc tttggggcaa gttttggttc tgcggaagtg
    17941 gcgataaacg ttgaaggtgc cgccgttgag cgagaaatga ataaaacggt tttgccgatg
    18001 atgcacggtt tttatagcct aggcacgctg gcaggcgctg gtgtcgggat ggcactgacg
    18061 gcctttggcg ttccggcaac ggtgcacatt ttattggcgg cgctggtagg tatcgcgcct
    18121 atttatatcg ccattcaggc aatccctgac ggtacgggca aaaatgctgc cgatggcacc
    18181 cagcatggcg aaaaaggcat tcctttttat cgcgatatcc agttgctgtt gattggtgtt
    18241 gtagtgctgg cgatggcctt tgccgaaggt tctgccaacg actggttacc cttattaatg
    18301 gttgacggtc acggttttag ccctacttcc ggctcgctga tttatgccgg ttttacccta
    18361 gggatgaccg ttggacgctt cactggcggt tggttcatcg accgttacag tcgcgttgcc
    18421 gtggttcggg ccagtgcact aatgggggcg ttgggtattg ggctgattat ttttgtcgat
    18481 agcgcctggg tcgctggggt gtctgttgta ctctggggac tgggtgcctc gctgggcttc
    18541 ccgctgacca tttctgccgc cagcgatacc ggccccgatg caccaacccg cgtcagtgtg
    18601 gtagcaacga ccggttatct ggctttcctc gtcgggccgc cgctgctggg ctatctcggc
    18661 gaacattatg gattacgtag tgcaatgctg gttgtactgg cgctggttat tctcgcggct
    18721 attgtcgcga aagccgtcgc caaacccgat accaaaacgc ggacggcgat ggagaatagt
    18781 tgatgagcat taaattaatt gcggtagaca tggatggtac tttcttaagc gatcaaaaaa
    18841 cctataaccg tgagcggttt atggctcagt atcagcaaat gaaagcacaa ggcattcgct
    18901 ttgtggtcgc cagcgggaat caatattatc agttgctctc tttcttccct gaaattgcta
    18961 atgaaattgc ctttgtggct gaaaacggcg gctgggtagt gagtgaaggc aaagatgttt
    19021 ttaatggcga gctgtcgaag gatgccttta ctactgtcgt ggaacatttg ctgacgcgcc
    19081 cggaagtgga aattattgcc tgcggaaaaa atagcgccta tacgctcaaa aaatatgacg
    19141 atgccatgaa aacggtggca gaaatgtatt atcaccgtct ggaatacgtc gataactttg
    19201 acaacttaga agatattttc tttaagtttg gcctgaatct ttccgatgaa ctgattccac
    19261 aagtacaaaa agcattacat gaggccatcg gcgatattat ggtgccagtc cacaccggca
    19321 acggcagcat cgatctgatt atccccggcg tacataaagc caatggcctt cgccaactgc
    19381 agaaattatg gggaatagac gacagcgaag tggtggtctt tggcgatggc ggtaacgata
    19441 ttgagatgct gcgtcaggca ggctttagtt ttgcaatgga aaatgccggc agcgcggtcg
    19501 tcgcagcggc aaaataccgg gcaggctcca ataaccgtga aggcgtactg gatgtgatcg
    19561 ataaagtgct taaacacgaa gcgccatttg accaataaaa acggcagcac gcccggcaac
    19621 acaatacctg aatgaaggct gcataattcg cggaaattaa cccgctgcat tatttcaggc
    19681 gaaatgatta tgaaaaattg tctactgttg ggcgcgcttt taatgggctt tactggtgtg
    19741 gcgatggcgc aaagtgtcac cggcgatgtg ccgagtggat acaaagtggt tgtagttcct
    19801 gattcagtca gcgttccaca agcggtcagc gtcgcgactg tgccgcaaac cgtctatgtg
    19861 gctcctgcgc cagctccggc ttaccgcccg catccttatg ctcgccatct tgccagcgta
    19921 ggtgaaggga tggttatcga acaccagatt gacgaccatc accattaatt cgagaatgcc
    19981 tgatcgcaca aagcagtcag gcattttttt acccttcgtg agaatttccc atctgtttat
    20041 cttttaaaaa gataaccatc agcgacagcc acaaaattcc gttgacaagg ttgaagagat
    20101 taaactgtcc gttgcccccg ttcagccagg catgtttgct gatttcaata ccaacggtaa
    20161 agatcagcat ttgcagcatt cccatcgccg cagaaaccgt acctttactc atatcgctgg
    20221 caaacagggt taatcgcacc agtcccgcat tcgccagacc aataccgaaa gcataaatac
    20281 ttaacccggc agtcatccat aaatacgcgt gcgatgagat aaccgttgcc gcagcagcga
    20341 ccaacagacc aatcataatc ggccagccgc ccataattat cacgtacggt gcggcgcgag
    20401 gtcagacgcg ctaacagcaa gttacccgca attaacgccc cgaaaatagg cacttgcagc
    20461 aagccatatt catagctgct caactgctcc ccggtaatga tgataatcgg cgactgggcg
    20521 atccacgcca gcaatggcag gctaacgaat cccagcgcca gcgcccccgc cacaaagcgg
    20581 ccgttcttca gcaccagctt atagtcacga ccgagttctt tcagtgacag tttctcgcct
    20641 atacgcgtgg cggtttcagg catagctcgt tgcagaccga aaaaggagat cgctgccaat
    20701 gcggcaaaca aaacaaacat cccctcccag ggcagcacat ggatccacgc cgcgcccacc
    20761 agcggaccaa gtagcggagc aatcagcgcc acgttcgcca tcagcgcggt gatcttgata
    20821 caaaccgcct cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc aatgaaacag
    20881 aggcttatgc cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg cgccagtaat
    20941 attgccagac aggtgacgat aaaccacacc acacccgcca gcatcaccgg acggcgacca
    21001 atacgatccg acagcggccc caggagccat tgtaaaaaca tcccgcccgc cagatacgcg
    21061 gtcatcgaag taggaaccca atcaatgccc gcctgatatt gttccaccac ggccaacata
    21121 ccgggttgga tcatatcgtt gccgatatag gttgaaaatt cgtaaagcac cagacagaga
    21181 gggaaaagta acgcctgacg tccaagcctg gcaccgaaag ctaatttatt ttgcatgcaa
    21241 tttcttcgcc aataataatc gcgcagagtt taataaaagc gcagctaacg agaaagcgaa
    21301 ttttgtagct gaaaccacgg ttaagcacat tcttacatta ttgcgagtat agctacgctt
    21361 tctttaagtt ttatttaacc tatgcccgtt acaatcaccc accgtaaaca ggccgcttga
    21421 gggaaataag acgatgccgc tttacccagt ttaacctgca ctttattctc aacgacttgc
    21481 ctgtattggc tcccttttaa tcactttgcg tcgggaagtt atgctggaaa atttgaatct
    21541 ctctctattc tctcttatta acgcgacgcc agactcggct ccgtggatga tctcgttggc
    21601 gatttttatt gctaaagatt tgattaccgt ggtgccgttg ctggccgcgg tactttggtt
    21661 gtgggggctt acagcacaac ggcaactggt gataaaaatc gctatcgcgc tggcggtcag
    21721 cctgtttgtg tcctggacga tgggacatct ttttccgcac gaccgaccct ttgtcgaaaa
    21781 tatcggctat aacttcctgc atcatgcggc ggatgactca ttcccgagcg atcacggtac
    21841 ggtgattttc acctttgcac tggcattttt atgctggcat cgcctgtggt ccggctcact
    21901 tttaatggtt ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg tccactggcc
    21961 gctggatatg ctcggtggat tgctggcagg tatgattggc tgccttagtg cccagattat
    22021 ctggcaagcg atggggcata aactctatca acgtctgcaa tcgtggtatc gcgtctgttt
    22081 tgcattaccg atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa gtgtagtatt
    22141 gagcggctcg cttcaataac tattcagagg gattatggaa acacgtcgcg aagagcgtat
    22201 cgggcagctg ctgcaagaat taaaacgcag cgataagtta catcttaaag acgccgccgc
    22261 cctgcttggg gtttcggaga tgacgattcg tcgcgatctg aacaaccaca gtgcgcccgt
    22321 cgttttgctc ggcggctata ttgttctgga accgcgcagt gccagccatt acctgttaag
    22381 cgatcaaaaa tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg ctgcgacact
    22441 ggtagaaccc gatcagaccc tcttttttga ctgtggcacc accacgccgt ggattattga
    22501 agcgattgat aatgaaatcc cttttaccgc cgtttgttat tcgctaaata cctttctggc
    22561 gctgaaagag aaaccccatt gccgcgcatt tctgtgcggt ggtgaatttc acgccagcaa
    22621 cgccattttc aaccccatcg attttcagca aacgttgaat aatttttgcc cggatatcgc
    22681 tttttattcc gcggcgggcg tgcatgtcag taaaggcgct acctgtttta atcttgaaga
    22741 gttgccggta aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg ttgtcgacca
    22801 cagtaaattt ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct ttgatattgt
    22861 ggtgagtgat tgttgcccgg aagatgagta tgtgaagtac gcgcagacgc accgtattaa
    22921 gttgatgtat taatgacgta taaccggatg acgtttcgcg ccatccggtt atcagaagat
    22981 taagagaacc agctgccgaa ccactgatgg aatttcatca tcacgaaatc ccacatccga
    23041 ccaaagaatc cgccctcttc cacattttcc atcacgatca gcggacgctg ctcaatggat
    23101 ttaccgttaa gctggaaatc aatggtcccg acaacctgac cttttttcag cggtgcggta
    23161 agctgcggtt ccgttaacgt gtagctcgcc ttcaggtttt tcagctgccc acgcggtatg
    23221 gtcacggagc ccgcttcgcc tgccccgaga ttcacttcgc tcttatcacc aaaccagacg
    23281 cgctgagtca caaaggtggc atcaggttta attggcgtca cagtttcaaa gaagcggaaa
    23341 ccccaggtca ataatttctc agactcatta aaacggatac ggtcagtttt cgcccccagc
    23401 actacggaga ttaaacgcat atcaccctgg gtagccgaag caaccagatt atatcccgca
    23461 cctgccgtgg ttcctgtctt catgccatca acattcagat tgctgctcca cagcagacgg
    23521 ttacggttag gctgacgaat tttgttgaag gtgaactctt tctctttatg aatggcgtac
    23581 tcttccggca catcgtggat caatgcttta cccagcaatg ccatatcgcg cgcggtactg
    23641 aactgccccg gcgcatccag accgtgcacc gtctggaagg tagtgttggt cagacccagt
    23701 tttttggcat aaccattcat caaaccaata aatgactcct ggctcccggc aacgtaatcg
    23761 gccagcgcaa tacaggcgtc attaccggac tggataatca cacctttgtt caagtctgcc
    23821 accgaaacct gatcgcccgg tttgaggaac attaccgatg aaccacgcag tgccggattt
    23881 cccgtcgccc aggcatcttt accgaccgtc accatatcgg tgagtttaat cttatcggcc
    23941 ttaagcgcct gcccaaccac atagctggtc atgattttag tcaggctcgc gggatccagt
    24001 ttctcatccg cgttgccttc tgccagcact ttaccgctgg cgtaatccat taaaatccat
    24061 gcacgcgcat ccacgctcgg cgcttcaacg gtttgttccg ccgcgaatgc cgttggggca
    24121 aaaaggaata aaaatgcaga acccgctgca agaccacgaa ggagagagga gtattgcgtc
    24181 ataaatgcca cccgagtatc cattcaaaaa aaatacgtta tatcgccgta tgataagttc
    24241 ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag tttttaaagt
    24301 ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag ctgttgctta
    24361 aaagttaact ttacttcacc atcggagtcc tgttcagcct gtggctgcct gtcggaaaag
    24421 gggtgagtat gattacgctg tggggtcgga ataattcaac taacgtaaaa aagtattgct
    24481 gacgctcgaa gaactggaac taccttatga gcaaattctc gcgggccgtg agtttgggat
    24541 aaatcacgat gctgattttc tggcgatgaa ccctaacggg ctggtgccgt tgttgcgtga
    24601 cgacgaaagt gatctcattc tttgggaatc aaacgccatt gtccgctatc tggcggcaca
    24661 gtacgggcaa aaacgcctgt ggatcgactc accggcacgt cgtgcggaag cggaaaaatg
    24721 gatggactgg gcaaaccaga cgctcagtaa tgctcatcgc gggatcctga tgggattagt
    24781 cagaacacca ccggaagagc gcgatcaggc cgccattgat gccagttgca aagagtgcga
    24841 cgccctgttt gccctgctcg atgcggaact ggcaaaagta aaatggttct ctggcgacga
    24901 gtttggtgtg ggcgatatcg ctatcgcacc gttcatctac aatttgttta acgttggcct
    24961 gacctggaca ccgcgtccaa atctgcaacg ctggtatcag caactcactg aacgccccgc
    25021 agtgcgcaaa gtggtgatga ttcccgttag ctaattgcgt gggctaactt taagtaattc
    25081 cccactggac tcgtcggtga gaacgtataa ataaccgtcg ggtccagtgc gaacatcacg
    25141 aattcgctgc cctctgtccg ttaaaatacg gccatcttct gtcactttgt cgccgttgac
    25201 gctcatcaca atgacatctt tatctttcag cgcgccaata aataattttt gctgccactg
    25261 ggggaattta tcgctgttat agaaggccat gccgctcaca gcgggcgaat ctttccagta
    25321 aaaaacaggt tgctcggtcc cggcgacgat ctcccctttc gcttccggta tcttaaagcc
    25381 tgaatagttg attccccagg ttgccagcgg ccagccgtag tttttgcctt tttgcgggat
    25441 attaatttca tcaccaccgc gcgggccatg ttcattcagc cacagtgcat tactccacgg
    25501 attcatcgcc attccttgcg gattacgaat gccataagac cagatctcgg cgcgcgcacc
    25561 ggattccttt ataaaaggat tatcatccgg gatttcgccc tggtcggtca gacgcaccag
    25621 tttgccctgt aatttatcca gatcctgcgc cgtcgggcgc tgattgtttt cgcccagagc
    25681 aataaaaaga taacctttac cgtcgaatac cagccgcccg ccaaaatggt tgccggtaga
    25741 cagttttggc atctggcgga agacggtgcg gaagtcggtc acttttgaga gatcatcact
    25801 taagcggcca taacccacag cagttccggc tttgccatca tcgccaactt cggaataact
    25861 taaccagatg cggcgagact gagcaaaatc aggcgctaaa accacgtcca gcaggccgcc
    25921 ctgcccgtgc gcccaaacgt ccggaactcc ggaaagcggc gcagataatc cttttcctgc
    25981 ttgccagtga cgcaactcgc cgccgcgcag agtgattaac ataccgtgat tatcgggtaa
    26041 aaaggccagt gcccagggat ggtcgagttt gtcttgcagt acttcgacat ttaccgttgc
    26101 aggagccgcc cagagagcgg aagaaagaca aataaggggc acaaggaaaa aggattgtcg
    26161 atgcataacg cctccggatt gactctggct taaagcgtag tcagtggagg agataacaaa
    26221 ttcattttta caaaaactta aacatgaagg gggagacgct ttctccccct tagttttcag
    26281 gccttctcaa gcatggcgtg cttctgcagg ctctggatac tcagcgttaa gctcatcaga
    26341 caattttcaa gcttatcggc gttgacggta ataacagtcg ggcaatcatg gtgcccactc
    26401 atcaaacata ctgcggctgt cgctaatgct tcttcagcat gatgaagagc actccactct
    26461 tcctgatcca gatgaagatt caaccgcagc gatttatcgt gcagttcgcg attcagttta
    26521 aaaaagttat ctcgtagatg attgctttcg ctgacggaca tgtatccttt tgccttcctc
    26581 agttgcacgt atgcagcgag gtcaacgcgt gcgtcgatca accggttcag cagatcgatt
    26641 cgctgtctgt caacgaacat acttcgatcc tcctctttcc agccttgttg cgattaaata
    26701 gtatggctat tttttgagca aaatgctgtc caggatcaat tcttcacgcc aaattaacaa
    26761 aatttacatt tccttcgcct ccgccagcga atgagattgc gatacaaatt ttatccagaa
    26821 ctctgtagaa tccctgccct gacctttcct gtaactgaat attttttaag ctatgagcaa
    26881 agtaactccc cagccgaaaa tcggctttgt ttcccttggc tgtccgaaaa accttgtcga
    26941 ttcagagcgt attctcaccg aactacgcac tgaaggttat gacgtggtac cgagctatga
    27001 cgatgcggac atggtgatcg tcaacacctg cggctttatt gacagcgcgg tacaagaatc
    27061 actggaagcc attggtgaag cgttgaatga aaacggcaag gtaattgtga ccggttgtct
    27121 gggggcaaaa gaagatcaga tccgcgaagt ccacccgaaa gtgctggaaa tcaccgggcc
    27181 tcatagctat gagcaggttc tggagcacgt tcatcactac gtgccaaaac cgaaacacaa
    27241 cccattcctg agcctggtgc cagaacaagg tgtgaaactg acaccgcgtc attatgccta
    27301 tctgaaaatt tcggaaggct gtaatcaccg ctgcaccttc tgcattattc cgtctatgcg
    27361 cggcgacctg gtgagccgtc cgattggcga agtgttaagt gaagcgaaac gtctggtaga
    27421 tgcgggcgtt aaagagattc tggtgatctc gcaggatact tccgcctatg gcgttgatgt
    27481 taaacatcgt actggcttcc acaacggcga gccggtaaaa accagcatgg tcagcctgtg
    27541 cgaacagtta tcgaaactgg ggatctggac acgtctgcac tacgtttacc cttatccgca
    27601 tgtggacgac gtcatcccac tgatggcaga aggcaaaatc ctgccgtatc tggacattcc
    27661 gttgcagcac gccagcccgc gcattctcaa actgatgaag cgtccgggtt ctgtagatcg
    27721 ccaactggcg cgcatcaaac agtggcgcga aatctgcccg gaactgaccc tacgctcaac
    27781 ctttattgtc ggcttcccta gcgagacgga agaagatttc cagatgctac tcgacttcct
    27841 gaaagaagcg cgtctggatc gcgttggctg ctttaaatac agcccggttg aaggtgcaga
    27901 cgccaatgcc ctgcctgacc aggttccgga agaagtgaaa gaagaacgct ggaaccgttt
    27961 catgcagctg cagcagcaga tttccgccga gcgcctgcaa gagaaagtgg gccgtgaaat
    28021 tctggtgatt atcgacgaag tggacgaaga aggcgcgatt ggtcgcagca tggcagatgc
    28081 accggaaatc gacggcgcgg tttatctcaa tggtgaaacc aacgttaagc cgggtgatat
    28141 cctgcgtgtg aaagtcgagc acgccgatga gtacgatttg tggggtagcc gggtttaatt
    28201 aattcaggcc gggcaaaatc atttgtccgc cctgcgaata atgattactg ccagctaatc
    28261 tgagccctgc cgtttttctt actcttatac agccgctcat ccgccagcat tttcaagcgt
    28321 tcgacggtgg tacattcatt tgaatgcgct acaccgatac tggtacttaa attaatgctt
    28381 cccacctgga aatgaacgat ttcttttaga atcgatagca actcttccag cgttccaccc
    28441 gtataaatca ccgcaaattc gtcaccgccg aagcggtaga ttttaccttt atctaccggg
    28501 atctgtgatt taaggatttt caccactttc atcaacactt catcgcccag caaatgaccg
    28561 aaggtgtcgt taatgttttt gaatttatcg acgtcaaaca ggcacaaata accgcctttt
    28621 tccggcagcg cttttaaatc ttcgtcaaag gattttctgc ccattgcttt ggtcaggtcg
    28681 tccaggaacg ccgagttata catactgtag agttttttga catcggcagc cgaaaagaaa
    28741 agcccgccat ccagtacgcg ttcaaaatgg ttattaatga tggcttcttt ctccacaata
    28801 ccacgattaa tcactctgcg taccagatag aggcaactgg tacccgatat cactgccacc
    28861 aatgcggcaa agaaaataat caatgtacag acccgcaaat aaacatcttt tttatacgca
    28921 aatacgtaca atccggtcgg caaataggcg aagtgctcca tcgcttccga aacgatgaag
    28981 atggcgtttt tagcattata ttcatcaccg gtttaccgtt cttaaataga gagaacatca
    29041 ggttctcgct gtccatcgcc agcgattttg acagtaaaga aaaacgctcc agaccaagca
    29101 aaaatatcag actacgattg tgagttaccg gatagaggta atagacgaag gttttaccgt
    29161 caaaagagat aaaccctgta tttttgacat gtcgatactt cacaacctgc ataatatctg
    29221 cacaataatt tttctcgaac tcctttacct gaatacattc gtcactggaa atcagataag
    29281 gaccgacatc gtacaaataa gagggggaaa ccgagtcggc gtaagctgtg cgcaaaccaa
    29341 ccaacagctg ttcctccgtg ggatttagcg tgttgataag atcaattgcc acgctacgcc
    29401 gatattcagc aatatttgcc accgcaaaag cagtatcaat aacggactgt ttcaggctat
    29461 acacccgttc ctttaccatt tgagtgtaga tcccgcaggc aactgctgaa atcatgataa
    29521 aaatcagcag actgactgta agtacgaact tattgattct ggacatacgt aaactactct
    29581 tttactaatt ttccactttt atcccaggcg gagaatggca tgggtttctg gtacagatac
    29641 ccctgagcct gaacacatcc taatttaatt aattcgcgcg caacgtcttc gctctctacg
    29701 ccttccgcaa tcaccgggat ccggttatag cgtgccagat cgaaaataat gcgcacaatt
    29761 tttcccgcag taccgaaaat atcgccggta aattgcgagt ccacctttat gctgtctgga
    29821 ttaatttcat aaaaacgttt caggtttgac aaaccggtgc caaagtcatc aatcgatatc
    29881 cgtacccctt gtttacgcag ttgttcaata ttgagataaa aatgttcatt aattcgctcg
    29941 atcacggttt cagtgatctc aacacagagg ctgttcgcct tcaggccatt ttctttcatg
    30001 atggtggtaa aacgctcatg aaagtgcggt tcgtttaact gccagggcga aacattaata
    30061 tgcagtaaga aatcatcctt cagcgacgca cgctgactaa aatgtctgaa ttcacccatt
    30121 gccgttttaa taatctgata acccagctca ttgataaacc cgatatcttc agcaatggta
    30181 atgaacttca gcggtgaaat gatcccccgc tccgcagaaa cccaacggca caacgcctcc
    30241 gcgcccagaa tggcgcgggt gttaatatca acgatcggtt gatagacgag gaatagttcg
    30301 ccaatttcca ttgcatggcg aatattacgc gcaatccaga tattctcttc tatttcgttg
    30361 agtttcgcgt catcacagaa aataaactcg gagaacatat ggtcacggat actggaaatc
    30421 gccagtttca gtttttctgc gtaactccag gcgttttccc cctgcaaagg ttcacaaatg
    30481 gcagacttat ggatgcgcag attgcggtag ccaaagctgc tgtcaaaatc gtttaccatc
    30541 gctattttac gggtaaatgt ctgtacattt tcccgacaca gaagtaaata gagatcggca
    30601 ttgaatgtca cgatatcgca gcattgcgca tatttctcgg caattcgctg acacaatttc
    30661 ctgacgattt tattgcgttc atcaacacca aacaccgctt ccacggcttc cagaccacac
    30721 agtttgatga tccccacagc cagatactga ttattgttgg actcaataat cgccatattt
    30781 ttaataaaac cgctttcctg atcgtaagag atcagtgaga gcaatttatt tgaccgttca
    30841 atttcggcat ctttcatttc cgcgatcgaa
//
LOCUS       DAZPEP010000035        30154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZPEP010000035 DAZPEP010000000
VERSION     DAZPEP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.035"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHUT9_001582"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHUT9_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFR1348085.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHUT9_001583"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHUT9_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFR1348086.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHUT9_001584"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHUT9_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFR1348087.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUT9_001585"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHUT9_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFR1348088.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUT9_001586"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHUT9_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFR1348089.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUT9_001587"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHUT9_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFR1348090.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUT9_001588"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHUT9_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFR1348091.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUT9_001589"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHUT9_001589"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFR1348092.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUT9_001590"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHUT9_001590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFR1348093.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUT9_001591"
     CDS             complement(12918..14051)
                     /gene="thiH"
                     /locus_tag="ACHUT9_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFR1348094.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUT9_001592"
     CDS             complement(14048..14818)
                     /gene="thiG"
                     /locus_tag="ACHUT9_001592"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFR1348095.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUT9_001593"
     CDS             complement(14820..15020)
                     /gene="thiS"
                     /locus_tag="ACHUT9_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFR1348096.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUT9_001594"
     CDS             complement(15004..15759)
                     /gene="thiF"
                     /locus_tag="ACHUT9_001594"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFR1348097.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUT9_001595"
     CDS             complement(15752..16387)
                     /gene="thiE"
                     /locus_tag="ACHUT9_001595"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFR1348098.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUT9_001596"
     CDS             complement(16387..18282)
                     /gene="thiC"
                     /locus_tag="ACHUT9_001596"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFR1348099.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18363..18463)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18516..18992)
                     /locus_tag="ACHUT9_001597"
     CDS             complement(18516..18992)
                     /locus_tag="ACHUT9_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rsd/AlgQ family anti-sigma factor"
                     /protein_id="HFR1348100.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKLGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUT9_001598"
     CDS             19087..19860
                     /gene="nudC"
                     /locus_tag="ACHUT9_001598"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFR1348101.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUT9_001599"
     CDS             19900..20964
                     /gene="hemE"
                     /locus_tag="ACHUT9_001599"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFR1348102.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUT9_001600"
     CDS             20974..21645
                     /gene="nfi"
                     /locus_tag="ACHUT9_001600"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFR1348103.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21688..22278
                     /locus_tag="ACHUT9_001601"
     CDS             21688..22278
                     /locus_tag="ACHUT9_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFR1348104.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUT9_001602"
     CDS             22465..22737
                     /gene="hupA"
                     /locus_tag="ACHUT9_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFR1348105.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22750..23445
                     /locus_tag="ACHUT9_001603"
     CDS             22750..23445
                     /locus_tag="ACHUT9_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFR1348106.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUT9_001604"
     CDS             complement(23447..23872)
                     /gene="zraP"
                     /locus_tag="ACHUT9_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFR1348107.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUT9_001605"
     CDS             24110..25486
                     /gene="zraS"
                     /locus_tag="ACHUT9_001605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFR1348108.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUT9_001606"
     CDS             25483..26808
                     /gene="zraR"
                     /locus_tag="ACHUT9_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFR1348109.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUT9_001607"
     CDS             complement(26805..28094)
                     /gene="purD"
                     /locus_tag="ACHUT9_001607"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFR1348110.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUT9_001608"
     CDS             complement(28106..29695)
                     /gene="purH"
                     /locus_tag="ACHUT9_001608"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFR1348111.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7057 a         7868 c         8307 g         6922 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctacgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ccccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgat caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacgc cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac atataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgct tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgctcgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggtaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaat ccctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttggtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggagctg gtgccggaac gcctgcgtgg tgaaactgca
     5101 tctttcgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ttggctaagc tgagccagtc tggtcacaag
     5341 cgtattgaaa cgctgttcac caacgacctg gatcacggcc cgtatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacctgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ctgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggac
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc cgggtggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcatt acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctt tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaggtga ccgacggtgt tgtaactgac gaaattcact acctttctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgtga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtacgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtttg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgaccgacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtgtatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcggggtaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggtgcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccactggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactact ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcgaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gagggtggtg acgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg taaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaaaggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaaca ccgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtgccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 ccagaccatt actcgtcaga ccgacgaact gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatgggtg ttattcagag ggcaaattct tcacggaaaa tattaagaaa ggcagggcga
    12781 aacgaggaag aagcttttcg ccctggacaa cattcctgct gacggcacta ccataaaaaa
    12841 acacaacgct ttcaacaacc ttgcgttttt catcttccgt agcggctcgg aataaattta
    12901 cgttcttaca ttccgtctca tagtctttgc gaggcgcgtc ccagatagct gtcccagtct
    12961 ttccataccg gctgcaaacc ctgagcggtt aacgcggcag caaccgcttc cggtctgcga
    13021 tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat cggcatagcc acctggctgc
    13081 gttttcgaga aggcgctgac gttattgatc gccagcggaa taacgcgatc gcgaaaccac
    13141 ggtgattccc gcgtggagag tgacagttca atctccggtg caagcagtcg gaaggcgcag
    13201 atggtttgca ctaactggcg ttcatccata atcgacgcag gctcaatgcc gccagtacac
    13261 gggcgcaggc gcggaaagga gacagagtaa cggctttgcc agtaatgctg ttgcagccat
    13321 agcaaatgtt cggcaaccat atagcagtca acgcgccagt tgtcggaaag gccaattagc
    13381 gcgccgaggc ctatcttatc aatccccgca cgccccagcc gatccggcgt ttccagccgc
    13441 cagaagaagt cctgtttttt gcctttcaaa tgatggcggg tataagtcgc ctcgtgatat
    13501 gtctcctgat aaaccatcac cccatccaga ccaagttgct ttaactcggc gtattccgtc
    13561 tccgccagcg gttgcacttc catttgtagt gaagagaact gttcacgcag ggcggggaga
    13621 tgacgacgaa agtaatccat ccccactttc gcctgatgtt caccagtgac taatagcaga
    13681 tgttcaaagc ccatctcccg tatagcggcg ctttccctgg caatatccgc ttcatccagc
    13741 gttttgcgct tgatgcgatt actcatggaa aatccgcagt acgtgcagtc gttagcgcaa
    13801 agattggaaa gataaagcgg gacgtagaaa ctaactgtgt tgccaaatcg ctgacgggtc
    13861 agacgctgcg cccgttgggc cagtggttcc agatagccac tggcggctgg cgataacagc
    13921 gccatcatat cgtcgcgggt gagttgcgag gcatttagcg cccgttctac gtcagcagcc
    13981 gttttgccgt tgatacgcag gcggatgtcg tcccagtcca gttgtcgcca gcgatcgctg
    14041 aaggttttca tgccgatgcc tccagaaatc cagtcagcgg gctggtggca tgagcaaaat
    14101 gactgcggct gcccggtccg gactgacgtg ccagcaggcc tgcttctacc gccagacgaa
    14161 atgccttcgc catgttgacg ggatcgtccg ctacggcaat cgccgtattc actaacaccg
    14221 cgtcggcccc catttccagg gcctgcgcgg catggctggg aacgccgatg ccagcatcga
    14281 caaccaccgg aactgtggcc tgctggataa taatctccag catggcacgg gtttccagtc
    14341 cctgattcga gccaatcggc gcgccgagcg gcatcaccgc tgcacagccg acttcttcca
    14401 gacgtttaca caataccgga tcggccccgc agtaaggcag cacgacaaat ccctgttgta
    14461 ccagcatttc ggcggctttc agagtttcga tgggatcggg caacagccag cgggcgtcag
    14521 ggtgaatctc taattttaac cagtttgtgc ctaacgcttc acgtgccaga tgggcggcga
    14581 aaatggcttc ttccgctgtt ttcgccccgg aagtatttgg cagcagggtc acacccgccg
    14641 cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa gtcgacacgt ttcatcgcca
    14701 gtgtcaccag ctggctgccg gaagcgcgga tcgcctccac catcagttgt gaagaagcga
    14761 atttccctgt gccggtaaac agatgtgaat caaacgtttt gtccgcaata cgtaacatct
    14821 caaccccctg caataacctg aaaaagcagg atctggtcgc catcctgcac gatatgttgc
    14881 gtccactgct cacgcgggac gatttgctga ttaatcgcca gagccgcgcc cgcttgtcgt
    14941 tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg cggcgcactg catcggttga
    15001 tcgttaaaca ggatctgcat tgcttcctcc gcataccggg caaccactgg cgcggcgcaa
    15061 cgccaggctg cgccactggc tcgatttacc gtcgaacagt cggagttctc ccgcaggtgt
    15121 ctctataccg cttaataact taatggcttc cagtgcctgc aaagtgccca taaccccgac
    15181 caccgggcca accacgcccg ccgtgcggca gttgcgttct ggctcctggt tatctggcca
    15241 caggcagcgg taacacccct gctcccaggg cggcgtcagt accatcaact gaccgccaaa
    15301 tccgaccgcg ctggcggtga taagcggcgt gttgagtacc acgcaggcgg cattaatctc
    15361 ctggcgagtc gccatattgt cggtacagtc gagcaccaca tcggcccgtg caaccgcatc
    15421 ttttaacgcc tcacccgtta accgttgttg taatgctgtc agttgaatat cgggattcaa
    15481 ctgtgtcagt cgctgatggc tgacctgcga tttcgggcga tcgatatctt cagtggtaaa
    15541 gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg tcatctgcca gtaccagcgt
    15601 cccgacgcca gcccccgcca ggtatagcgc agcaggtgta cccagcccgc caagaccaat
    15661 aatcagcacc tggctgtcga gcagtttttg ctgcccgtcc agagcgatat cgtcgagcag
    15721 aatttggcgg ctataacgca taaagtcacg gtcattcatc gccaactcct gcaatttcca
    15781 gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt aatggcgctg acgacggcga
    15841 tactgccgac acccgttgct atcaccgcag gcgcgcgcgc cagactgata ccgccaatcg
    15901 ccacggtggg ataatccgcc agtcgctcaa catgccgtgc cagctgttcc agcccctgcg
    15961 gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg tcccagcgcg atataagagg
    16021 ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg tgtcgaaacg cccagccgca
    16081 ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa atcttcctgc cccaaatgga
    16141 cgccatacgc ctgatgcttg atcgccagcc gccagtaatc gttgataaac aatcgcgcgt
    16201 tatagcggcg gcccagcgca attgccgcca cgacatcggc ttccacctct tcattgcgcc
    16261 gatctttgat gcgtagctgg agagtacgta cgcctgcatc caacagacgt tcgatccact
    16321 gtacgctgtc caccaccggg tacagtccta aacgaaaagg tacaggagga aaatcaggct
    16381 gatacatcac gcttcctcct tacgcaggta gatttctccg cctctggcgc ggaagttctc
    16441 cgacatatcc gccattccca cttcaatggt ttgcgcggcg gcgtaatcac gtacttcctg
    16501 gctgattttc atcgagcaga atttcggccc acacatggag caaaaatggg cgactttacc
    16561 tgactcttgc ggcagggttt catcgtgata agcgcgggcg gtaaacgggt cgagggccag
    16621 attaaactgg tcttcccagc gaaattcgaa gcgggctttc gacatggcgt tatcgcgaat
    16681 ttgcgcgcct ggatgccctt tcgccaggtc agcggcgtgg gcggcaatct tataggtgat
    16741 aagcccctgc ttaacatctt ctttattagg cagacccaga tgctcttttg gcgttacgta
    16801 acagagcatc gcgcagccaa accagccaat catcgccgca ccaatccccg acgtgaagtg
    16861 gtcatagccc ggcgcaatat cggtagttag cggccccaga gtgtaaaacg gcgcttcgtg
    16921 gcagtgctct aactcctcgg tcatattgcg gcggatcatc tgcatcggca cgtggcctgg
    16981 gccttcaatc atcacctgca cgtcatattc ccaggcaatt ttggtcagtt cgcccagcgt
    17041 atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga atagaaccgg ggcgcagacc
    17101 gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt tcacaaattt cgcggaagtg
    17161 ttgatagagg aaattttcct gatgatggga gaggcaccat ttcgccataa tcgaaccgcc
    17221 gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc acatagcgca gcagtacgcc
    17281 cgcatggata gtgaagtaat ccacaccttg ctcggcctgt tccagcagcg tgtcgcggaa
    17341 cgcttcccag gtaagatctt cggcgatccc gttaaccttc tccagcgcct ggtagatcgg
    17401 cactgtaccg atcggcaccg ggctgttacg caaaatccac tcgcgggttt cgtgaatata
    17461 gcgaccggtg gagagatcca tcaccgtatc cgctccccag cgcgtggacc ataccagctt
    17521 ttccacttct tcttcgatgg aagaggtgac cgccgagttg ccgatattgg cgttaacttt
    17581 taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc ggatgattaa tgttggccgg
    17641 gataatcgca cgtccggcag caacttcatc acggacaaat tccgcagtga tattttccgg
    17701 cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt aaaacctcgc tacggatgcg
    17761 ctcgcggccc atattctcgc ggatggcgat gaattccatt tccggcgtga tgatgccctg
    17821 gcgggcgtag tgcagttggg tgacacggcg tcctgttttg gcgcgttttg gcgttaataa
    17881 gccgctaaaa cgtagctcgt cgagaccatc atctgccagc cgcgttctgg tgtaatcgga
    17941 actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg atccacggct ggcgtagttt
    18001 tgccagccct tgctgcacgt taatggcgat ctgcggatca ccatacgggc cggaggtgtc
    18061 gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt tctttgctac cgccaattag
    18121 cgtcgggcta agctggatct cacgcatcgg cacgcgcacg ccggggtgtg tgccagtgat
    18181 ataaatgcgt tttgagttgg gaaaggcggt gccttccagg gtgtcgataa aatgttgggc
    18241 ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac atagctcatt ccaaaaagtc
    18301 aaggacgtgg cttgtcagac gacggatgaa gcaagagacg atcgccgcaa aggcgatgcg
    18361 atagcagatt aactcttgtt cccttcgcag gtattagcct gatcaggttc cgcggatccc
    18421 gaataaacgg tctcagccag ttaaggcact ccgacaagaa attagccccc acaaacgggg
    18481 cgttgaatgt aaattacgcg ttaacagcgc agaactcaag caggatgttt gacgcgggcg
    18541 gcgtcaagca ccagcagaat cagcttatct tccagcacaa agcgcgcttc cagcgcctcg
    18601 ccgatgtcag ataaaacctg ttgaaactca aggtaattat catgatcgat ggcggtttcc
    18661 aggctggaat cgtagtaatc cataatctgt tgggtattgg cttcgagttg cggccaaatc
    18721 ttcgcggcgc gcgcgagttg cccgttgcct tccagcttat gaagaatgcg ctcataaata
    18781 ctgaaatgtc cggcagacaa gtaatcgacc aggctctgac aaaaatcatc aagggctttt
    18841 tcgtttagcc tcatgtacga ttctttgcca agcttaatgc caaccagatt gtagtaagcc
    18901 acgagcagat gcttacgtac atgtagccag cgatcaacca gtttgttact tcctctgacg
    18961 cgctccgtca ggttatcgag ctggttaagc atgattgact ccgcaagttt gtattcaaaa
    19021 actgctcagt gagaaatgta aaaaccatgt taaacatgcc agtgatgcaa aggtagtgca
    19081 agagctatgg atcgtataat tgaaaaatta gatcacggct ggtgggtcgt cagccatgaa
    19141 caaaaattat ggttgccgaa gggagaattg ccatatggcg aagcggcaaa tttcgatctt
    19201 gtgggtcagc gcgcactaca aatcggcgaa tggcaggggg aacctgtttg gttagtacaa
    19261 cagcagcggc gtcacgatat ggggtcggta cgtcaggtca ttgatctcga tgttgggctg
    19321 tttcaactgg ccggacgagg cgtacaactg gcggagtttt accgatcgca taaatactgt
    19381 ggttactgcg ggcatgaaat gtatccgagc aaaaccgaat gggcgatgct gtgcagccat
    19441 tgccgtgagc gttactaccc gcaaatcgcc ccctgcatta ttgttgccat ccgtcgcgat
    19501 gattcgatcc tcctcgccca gcatacccgc catcgtaacg gtgtccatac agtacttgcc
    19561 ggattcgtcg aagtgggcga aaccctcgag caggcagtcg cgcgggaagt gatggaagag
    19621 agcggaatta aagttaaaaa cttgcgttac gtgacttctc agccgtggcc gtttcctcag
    19681 tctttaatga ccgcgtttat ggcggaatat gacagcggcg acatcgtgat cgacccgaaa
    19741 gaattgctcg aggcgaactg gtatcgctat gacgatttgc cgttactccc gccgcccggc
    19801 accgtagcgc gccgtctgat agaagatact gtggcgatgt gtcgggcaga gtatgagtga
    19861 tgatacactg accgcctgac gcactaagga acagccaaaa tgaccgaact taaaaacgat
    19921 cgttatctgc gggcgctgct gcgccagccc gttgatgtca ctccagtatg gatgatgcgc
    19981 caggcgggtc gctatctacc ggaatataaa gccacgcgcg cccaggcggg cgattttatg
    20041 tcgctgtgca aaaacgccga gctggcgtgc gaagtgactt tgcaaccgct gcgtcgctac
    20101 ccgctggatg cggcgatcct cttttccgat atcctcaccg tgccggacgc gatggggtta
    20161 gggctctatt ttgaagccgg agaaggtccg cgttttacct cgccagtcac ctgcaaagcc
    20221 gacgtcgata aactgccaat tccggacccg gaagatgagc tgggttacgt gatgaacgcg
    20281 gtgcgtacca ttcgtcgcga actgaaaggc gaagtgccgc tgattggttt ttccggcagc
    20341 ccgtggacgc tggcgaccta catggtggaa ggcggcagca gcaaagcgtt caccgtgatc
    20401 aaaaaaatga tgtatgccga tccgcaggcg ctgcacgctc tactcgataa actggcgaaa
    20461 agcgtcactt tgtatctgaa tgcgcagatt aaagccggtg ctcaggcagt gatgattttc
    20521 gacacctggg gcggtgtgct taccgggcgc gattatcaac agttctcgct ctattacatg
    20581 cataaaattg ttgatggttt actgcgtgaa aacgacggtc gccgcgtgcc agtcacgctg
    20641 tttaccaaag gcggcggaca gtggctggaa gcgatggcag aaaccggttg cgatgcgttg
    20701 ggcctcgact ggacaacgga tatcgccgat gcgcgccgcc gtgtgggcaa taaagtcgcg
    20761 ttgcagggta atatggatcc gtcgatgctg tacgcgccgc ctgcccgcat tgaagaagaa
    20821 gtagcgacta tacttgcagg tttcggtcac ggcgaaggtc atgtctttaa ccttggtcac
    20881 ggcattcatc aggatgtgcc gccagaacat gctggcgtgt tcgtggaggc agtgcatcga
    20941 ctgtctgaac aatatcaccg ctaaggagtg attatggatc tcgcgtcatt acgcgctcaa
    21001 caaatcgaac tggcttcttc tgtgatccgc gaggatcgac tcgataaaga tccaccggat
    21061 ctgatcgccg gagccgatgt cgggtttgag cagggcggag aagtgacgcg agcggcgatg
    21121 gtgctgctga aatatccctc gcttgagctg gtggagtata aagttgcccg catcgccacc
    21181 accatgcctt acattccagg ttttctttcc ttccgcgaat atcctgcgct gctggcagcg
    21241 tgggagatgc tgtcgcaaaa gccggattta gtgtttgtcg atggtcatgg gatctcgcat
    21301 cctcgccgtc ttggcgtcgc cagccatttt ggtttgatgg tggatgtgcc aacgatcggc
    21361 gtggcgaaaa aacggctctg cggtaaattc gaaccgctct ccagcgaacc gggcgcgctg
    21421 gccccactga tggataaagg cgagcagctg gcctgggtct ggcgcagcaa agcgcgctgt
    21481 aacccgttgt ttatcgctac cggccatcgg gtcagcgtgg acagcgcgct ggcgtgggta
    21541 caacgctgca tgaaaggcta tcgtctgccg gagccaacgc gctgggcgga cgcggtggcc
    21601 tcggaacgtc cggcgttcgt gcgctataca gcaaatcagc cctaattcag gtaaactgcg
    21661 gataatttcc gtatttgaga actcaacatg ttacaaaacc caattcatct gcgtctggag
    21721 cgcctggaaa gctggcagca tgtcactttc atggcttgct tatgcgaacg catgtacccc
    21781 aattacgcca tgttctgcca gcaaaccggt tttggtgatg ggcaaattta ccgtcgtatt
    21841 ctcgatctca tctgggaaac gctgaccgtt aaagacgcaa aagtaaattt cgacagccaa
    21901 ctggagaaat ttgaagaagc gattccttca gccgacgatt tcgatctgta cggcgtttat
    21961 ccggcaatcg atgcctgcgt ggcgttaagt gaactggtcc attcgcgttt gagtggtgaa
    22021 acgctcgaac acgcggtgga agtgagtaag acctccatca cgaccgttgc gatgctggaa
    22081 atgactcagg ctggtcgcga aatgagcgat gaagagctta aagaaaaccc agctgtagag
    22141 caagaatggg acattcagtg ggaaatattc cgacttttag ctgagtgcga agaacgcgat
    22201 atcgagctga taaaaggcct tagggcagac ctgcgtgagg cgggtgagag caatattggt
    22261 ataatttttc agcaataaga ccagaaaacg tgatttaacg cctgatttgt cgtacctgga
    22321 gtcttccctt tcgccccccg tctggtctac atttgggggg cgaaaaaaag tggctatcgg
    22381 tgcgtgtatg caggagagtg ctattctggc atttccgtcg cactcgatgc ttagcaagcg
    22441 ataaacacat tgtaaggata acttatgaac aagactcaac tgattgatgt aattgcagag
    22501 aaagcagaac tgtccaaaac ccaggctaaa gctgctctgg agtccactct ggctgcaatt
    22561 actgagtctc tgaaagaagg cgatgctgta caactggttg gtttcggtac cttcaaagtg
    22621 aaccaccgcg ctgagcgtac tggccgcaac ccgcagaccg gtaaagaaat caaaattgcc
    22681 gcagctaacg taccggcatt tgtttctggc aaggcactga aagacgcagt taagtaagat
    22741 tgcgtggcag tgaacagttt taacgaaggg gtggtttcac cccttttgtc tttctggcgt
    22801 cgatcattga tgctggctgg cgctctgctt ctcactgcct gtagtcataa ctcttcactt
    22861 cctcccttta ccgccagtgg atttgctgaa gaccagggcg cggtacgcat ctggcgaaaa
    22921 gacagcggcg ataatgtgca tctgcttgcc gtgtttagcc cgtggcgcag tggcgatacc
    22981 acgacgcgag agtatcgctg gcagggcgat aacctcacgc tcatcaatat caatgtttac
    23041 agcaaaccgc cggtgaatat tcgcgcgcgt tttgacgatc gcggtgatct gagctttatg
    23101 caacgtgaat ccgatgggga aaagcagcag ctttctaacg accaaatcga tttataccgt
    23161 tatcgtgctg atcagatccg ccagattagc gatgccttac gtcaggggag agtcgtgctg
    23221 cgccaggggc gctggcatgc gatggaacag accgtgacca cctgcgaagg gcaaaccatt
    23281 aaacctgatt tagattcgca ggcgatagcg catatcgagc gccgccagag ccgctcttct
    23341 gttgatgtca gcgtggcatg gctggaagcg cccgaaggtt cgcaattact gttagtggca
    23401 aactctgatt tctgtcgctg gcaacccaac gagaaaacgt tctgatttac cagtggccca
    23461 tacccatatg accgccacca ccgcagccgc cgtagcccat gcccattccg gctccgcgcg
    23521 gaatacccgc ttcagccatc gcgatatctc gtttcacccg taactcatct aacgactgac
    23581 gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt gggttcgcgg
    23641 ctaacagggc attgtattca taacgcttcg tcaccagttg ctgttgcagt gcgctgcttt
    23701 gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg gtcaaaggcg
    23761 cggcattttg ctgccacata ccgtgtccgc cgtgagcaaa tgcagatgtc gatcccatcg
    23821 ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc atggttaatc
    23881 ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacgatga aacgctgata
    23941 tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg ggtagcgagt
    24001 catttttact cattgaaact taagcctttg tgttacagcg cagggtaagc gctgataaaa
    24061 gatggcatga tttctgctgt cagaaaggga tgagcaggca aagaagaaga tgcgttttat
    24121 gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg tggtcattgt
    24181 tgggctggta gggttgtttg cggtgacggt gattcgcgat tatgggcgcg agactgccgc
    24241 cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgcgctcttg aatccggctc
    24301 gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac tggaagaaat
    24361 ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa caatcgtgat
    24421 gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa tgcagcagtt
    24481 acatccggga gatgaagaag cgtggcggcg gatcgatagc gcagacggcg agcctgttct
    24541 ggaaatttat cgccagtttc aaccgatgtt tgctgctgga atgcaccgga tgcgccatat
    24601 gcagcagtat gccgcgacac cacaagcaat tttcatcgct ttcgacgcca gtaatattgt
    24661 gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc tggcgacggt
    24721 cttgctggca agcgtattgt cattcttctg gtatcgccgc tatttgcgct cgcgccagct
    24781 tctacaagat gaaatgaagc gcaaagagaa gctggtggcg ctggggcatc ttgcggcagg
    24841 cgttgcccac gaaatccgta acccactttc ctcgattaaa ggactggcga aatactttgc
    24901 cgagcgcgcg cctgcagggg gagaagcgca tcaactggcg caggtgatgg cgaaagaggc
    24961 cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa cgcatctggc
    25021 tttgcaggcg gtggatctca acacgctgat taaccactca ttacagctgg taagccagga
    25081 tgcgaacagc cgggagatcc agttacgctt taccgccaac gacacattac cggaaattca
    25141 ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaatg ctattcaggc
    25201 gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg gcgtgaaaat
    25261 cagcgttacc gacagcggta agggaattgc ggcagatcag cttgatgcca tcttcactcc
    25321 gtacttcacc actaaagccg aaggcaccgg actggggctg gcggtcgtgc ataatattgt
    25381 tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg caacgttcac
    25441 cctctggctt ccggtcacta ttacgcgtaa gggcccacaa ggatgacgca cgataatatc
    25501 gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc tttactgcgc
    25561 ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga gcaggtgcgg
    25621 gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cggagatgga cggcattgcc
    25681 acgctaaaag agatcaaagc gttaaatccg gcgattccgg tgctgattat gaccgcatac
    25741 tccagcgtcg agacggcggt agaagcgttg aaaaccgggg cgctggatta tctcatcaag
    25801 ccgctggatt tcgataacct gcaggcgacg ctggaaaagg cgctcgcgca tacgcacagt
    25861 attgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg taaaagcccg
    25921 gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc cacggtgctg
    25981 atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca cgccagtagc
    26041 gcacgtagcg aaaaaccgct ggtaacgctc aactgtgcgg cactcaacga atccttgctg
    26101 gaatctgaat tgttcggtca cgaaaaaggg gcgttcactg gagccgatag acgccgggaa
    26161 gggcgctttg ttgaggcgga cggcggcacg ctgtttctcg atgaaattgg cgatatctcg
    26221 ccgatgatgc aggtgcgtct gctgcgtgcg attcaggagc gcgaagttca gcgtgtcggt
    26281 agcaaccaga ctatctcggt tgatgtccgg ttgattgcgg cgacccatcg cgatcttgcc
    26341 gcagaggtga atgccgggcg ttttcgccag gatctctact atcgcctgaa tgtggtggcg
    26401 attgaagtac catcgctgcg gcaacggcgg gaagatatcc ctctgctggc tgtccatttt
    26461 cttcagcgct ttgccgagcg taatcgcaag gcggtaaaag gctttacgcc acaggcgatg
    26521 gatttgctga tccactacga ctggccggga aatattcgtg aactggaaaa cgcggtggaa
    26581 cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc gctggcgatt
    26641 gccggtacgc cgatcccact ggggcaaagt caggatattc agccgctggt ggaagtcgaa
    26701 aaagaagtga ttctggcggc actggagaaa acgggcggca acaaaaccga agccgcccgt
    26761 cagttaggga tcacgcgcaa aacgctattg gcaaaactgt cgcgttagtt ctgctcgcgt
    26821 tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccagtg aatatcggtc
    26881 attaaggcat aagcgcgttt ctgcgcttct gccacggtat gacccagcgc ggtgacgcac
    26941 agtacgcgcc cgccgctggt cactacctgc tcgttatccg ccagtttcgt gcccgcgtgg
    27001 aacactttgc cgcctgccac ttcttccagc ggcaggccgt ggatcacatc gccggtgcgg
    27061 taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcacg ctcatcccac
    27121 tctgacgttt tctcgtccag tttgccttca caggccgcca ggcagagctc aaccagatcg
    27181 gacttcatgc gcagcataat cggctgggtt tctggatcgc caaagcggca gttaaattcg
    27241 ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa accggtgtag
    27301 gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc catggtgcgc
    27361 tgatgaactt catcggttac taccggcgcg ggggagtaag cgcccatccc gcccgtgttt
    27421 ggtccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat cggcagcaca
    27481 tgctcgccgt ctaccatcac gataaagctc gcttcttcgc cgtcgaggaa ctcttcgata
    27541 acgatgcgat gacccgcgtc gccaaaagcg ttgcccgcca gcatatcgtg aacagccgct
    27601 tcggcttctt ccagcgtcat cgccacgata acgcctttcc cggctgccag accgtccgct
    27661 ttaatgacga tcggcgcacc tttctcacgc agatacgcca gcgcaggttc tacctcggtg
    27721 aagttctggt attccgctgt agggatctta tggcgggcca ggaaatcttt ggtaaacgct
    27781 ttcgaacctt ccagttgcgc ggcacctgcg gttgggccga agattttcat cccggcggcg
    27841 cggaaggtat cgaccacgcc tttcaccagc ggcgcttccg ggccgacgat ggtcagatca
    27901 atcttttcgt tttgtgcgaa atccaacagc gccgggatat cggtcacgcc gatagcaacg
    27961 ttttgcagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa aacagtttca
    28021 accagcggcg actgcgccgc tttccaggcc agcgcgtgct cgcgcccgcc gttaccaatc
    28081 actaatactt tcatctattg ctccattaat ggcggaagtg gcgcatgtcg gtgaagagca
    28141 tcgcaatacc gtgctcgtcg gcggcggcaa tcacttcgtc atcacggata gaaccgccag
    28201 gctggattac gcaggtcacg cctgcagcgg cggcggcatc aataccgtcg cggaacggga
    28261 agaacgcgtc agaagccatc gaggaacctt tcacttccag gccttcatcg gcagctttaa
    28321 taccggcgat tttcgcggag tacacgcggc tcatctggcc cgcgccaatc ccgatggtca
    28381 tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact ttccagcaga
    28441 acagcgcatc acgcagttcc tgttcggtcg gctgacgttg ggtgacgacg cgcagttctt
    28501 ccgcaccgac catgcccagg tcacgatcct gaaccagcag accgccgttc acgcgtttga
    28561 aatcaagacc cggaacacgc tcgccccact gaccgcaggt cagaacgcgt acgttctgct
    28621 tggcggcggt gattttcagg gcttcttcgc tggcggaagg cgcaataatc acttcgacaa
    28681 actgacgaga aatgatggcc tgtgcggttt ccgcatccag ctcgcggtta aaggcaatga
    28741 tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca tcaagaatgg
    28801 aattgccgat agccacgccg caagggttgg cgtgcttcac aatcacacat gccggctcgg
    28861 cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta taagagaggg
    28921 ctttaccctg aacctgggtt gcggtagcaa cggaggcttc tttcacattc tcttctatat
    28981 agaaggcagc ctgctggtgg ctgttctcgc cgtaacgcat atcctgcttc ttaatgaagt
    29041 tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gctttcaccg tggtaagccg
    29101 gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcagtg tgttcgaagg
    29161 ctttgatggc gaggtcgaaa cgggttgcaa gcgtcagcga tccttcgttg tcatccatct
    29221 ctttaataat ggcgtcgtag tcgctgctct tcaccacgat tgcgacatct ttatggttct
    29281 tagcggcgga gcgcaccatg gtcgggccgc cgatatcgat gttctcaacc gcatcttcca
    29341 gcgagcagcc ttcacgagca acagtctggg cgaacggata caggttaaca accaccatat
    29401 cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggccgcga cggcccagaa
    29461 tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc tccgggaaac
    29521 cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga cgggcagtgc
    29581 cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat tcgacgatac
    29641 cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt tgcatggtaa
    29701 atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa aaactcagct
    29761 aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac gtttgcgcaa
    29821 cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggtt accgtgggaa
    29881 aaatacagaa attacattga tgattgtgga taactctgtg cgtaaaaagg tataaagcgg
    29941 ggttttgctg gggaatgcag cagtcagtca tttttctgca atttttctat tgcggcctgc
    30001 ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact tcacacaaac
    30061 agccggttcg gttgaagaga aaaatcctga aattcagggt tgactctgaa agaggaaagc
    30121 gtaatatacg ccacctcgcg acagtgcgct aaag
//
LOCUS       DAZPEP010000036        29548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZPEP010000036 DAZPEP010000000
VERSION     DAZPEP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.036"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="ACHUT9_001609"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="ACHUT9_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dGTP triphosphohydrolase"
                     /protein_id="HFR1348112.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="ACHUT9_001610"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="ACHUT9_001610"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFR1348113.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUT9_001611"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="ACHUT9_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFR1348114.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="ACHUT9_001612"
     CDS             complement(3644..4030)
                     /locus_tag="ACHUT9_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFR1348115.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUT9_001613"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="ACHUT9_001613"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HFR1348116.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUT9_001614"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="ACHUT9_001614"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFR1348117.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUT9_001615"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="ACHUT9_001615"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFR1348118.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHTPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUT9_001616"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="ACHUT9_001616"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFR1348119.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUT9_001617"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="ACHUT9_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFR1348120.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUT9_001618"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="ACHUT9_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFR1348121.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUT9_001619"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="ACHUT9_001619"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFR1348122.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="ACHUT9_001620"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="ACHUT9_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFR1348123.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUT9_001621"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="ACHUT9_001621"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFR1348124.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUT9_001622"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="ACHUT9_001622"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFR1348125.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUT9_001623"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="ACHUT9_001623"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1348126.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUT9_001624"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="ACHUT9_001624"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFR1348127.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUT9_001625"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="ACHUT9_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFR1348128.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="ACHUT9_001626"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="ACHUT9_001626"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFR1348129.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUT9_001627"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="ACHUT9_001627"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFR1348130.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUT9_001628"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="ACHUT9_001628"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFR1348131.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUT9_001629"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="ACHUT9_001629"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFR1348132.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUT9_001630"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="ACHUT9_001630"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFR1348133.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUT9_001631"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="ACHUT9_001631"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFR1348134.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUT9_001632"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="ACHUT9_001632"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFR1348135.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="ACHUT9_001633"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="ACHUT9_001633"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFR1348136.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8161 g         7108 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggg tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgacaaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcccgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttcttgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAZPEP010000037        29478 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZPEP010000037 DAZPEP010000000
VERSION     DAZPEP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29478
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.037"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..644)
                     /locus_tag="ACHUT9_001634"
     CDS             complement(<1..644)
                     /locus_tag="ACHUT9_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFR1348137.1"
                     /translation="MVKKPQQGTIFAAAQRSDSYFVKCINMLSLYEKIKIRLIILFLL
                     AALSFIGLFFIINYQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLLK
                     NTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDES
                     GRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
     gene            complement(744..1655)
                     /gene="gsiD"
                     /locus_tag="ACHUT9_001635"
     CDS             complement(744..1655)
                     /gene="gsiD"
                     /locus_tag="ACHUT9_001635"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFR1348138.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1658..2578)
                     /gene="gsiC"
                     /locus_tag="ACHUT9_001636"
     CDS             complement(1658..2578)
                     /gene="gsiC"
                     /locus_tag="ACHUT9_001636"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFR1348139.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2596..4134)
                     /gene="gsiB"
                     /locus_tag="ACHUT9_001637"
     CDS             complement(2596..4134)
                     /gene="gsiB"
                     /locus_tag="ACHUT9_001637"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFR1348140.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4154..6025)
                     /gene="gsiA"
                     /locus_tag="ACHUT9_001638"
     CDS             complement(4154..6025)
                     /gene="gsiA"
                     /locus_tag="ACHUT9_001638"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFR1348141.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6012..6977)
                     /gene="iaaA"
                     /locus_tag="ACHUT9_001639"
     CDS             complement(6012..6977)
                     /gene="iaaA"
                     /locus_tag="ACHUT9_001639"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFR1348142.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7181..8416
                     /gene="moeA"
                     /locus_tag="ACHUT9_001640"
     CDS             7181..8416
                     /gene="moeA"
                     /locus_tag="ACHUT9_001640"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFR1348143.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8416..9165
                     /gene="moeB"
                     /locus_tag="ACHUT9_001641"
     CDS             8416..9165
                     /gene="moeB"
                     /locus_tag="ACHUT9_001641"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFR1348144.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9344..10006)
                     /gene="fsa"
                     /locus_tag="ACHUT9_001642"
     CDS             complement(9344..10006)
                     /gene="fsa"
                     /locus_tag="ACHUT9_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFR1348145.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            10137..11036
                     /locus_tag="ACHUT9_001643"
     CDS             10137..11036
                     /locus_tag="ACHUT9_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFR1348146.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            11042..13474
                     /locus_tag="ACHUT9_001644"
     CDS             11042..13474
                     /locus_tag="ACHUT9_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFR1348147.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHNCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13620..14435
                     /gene="ybiV"
                     /locus_tag="ACHUT9_001645"
     CDS             13620..14435
                     /gene="ybiV"
                     /locus_tag="ACHUT9_001645"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFR1348148.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALNGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14587..15852
                     /locus_tag="ACHUT9_001646"
     CDS             14587..15852
                     /locus_tag="ACHUT9_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFR1348149.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15819..16979)
                     /locus_tag="ACHUT9_001647"
     CDS             complement(15819..16979)
                     /locus_tag="ACHUT9_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1348150.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQCAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            17217..18518
                     /locus_tag="ACHUT9_001648"
     CDS             17217..18518
                     /locus_tag="ACHUT9_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1348151.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWVHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18533..20896
                     /locus_tag="ACHUT9_001649"
     CDS             18533..20896
                     /locus_tag="ACHUT9_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HFR1348152.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLDFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(21076..22671)
                     /locus_tag="ACHUT9_001650"
     CDS             complement(21076..22671)
                     /locus_tag="ACHUT9_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFR1348153.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22890..23810
                     /gene="ldtB"
                     /locus_tag="ACHUT9_001651"
     CDS             22890..23810
                     /gene="ldtB"
                     /locus_tag="ACHUT9_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1348154.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23869..24987)
                     /locus_tag="ACHUT9_001652"
     CDS             complement(23869..24987)
                     /locus_tag="ACHUT9_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFR1348155.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24984..25451)
                     /gene="mntR"
                     /locus_tag="ACHUT9_001653"
     CDS             complement(24984..25451)
                     /gene="mntR"
                     /locus_tag="ACHUT9_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFR1348156.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25637..25765
                     /gene="mntS"
                     /locus_tag="ACHUT9_001654"
     CDS             25637..25765
                     /gene="mntS"
                     /locus_tag="ACHUT9_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFR1348157.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            26037..27620
                     /gene="opgE"
                     /locus_tag="ACHUT9_001655"
     CDS             26037..27620
                     /gene="opgE"
                     /locus_tag="ACHUT9_001655"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFR1348158.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27669..28184)
                     /gene="ompX"
                     /locus_tag="ACHUT9_001656"
     CDS             complement(27669..28184)
                     /gene="ompX"
                     /locus_tag="ACHUT9_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFR1348159.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(28237..28302)
                     /locus_tag="ACHUT9_001657"
     CDS             complement(28237..28302)
                     /locus_tag="ACHUT9_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348160.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28537..29424
                     /gene="rhtA"
                     /locus_tag="ACHUT9_001658"
     CDS             28537..29424
                     /gene="rhtA"
                     /locus_tag="ACHUT9_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFR1348161.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         7145 a         8075 c         7388 g         6870 t
ORIGIN      
        1 aacaaatcgt gatctttgcc gttgatacgt agcgtaaaag agatacattt tttacccgga
       61 cgatcaaagc aattgtatgc cggattccag ctgttgttag agtcatctga agccaccgac
      121 cacggtcgtt tcgatgggtt aaaatcggca aaaataaccc gccctgactc gtcgacaagc
      181 ggtccattta cttgttcctg atgataaaca acgattttat cattcgccct ccgcgaaaag
      241 agataatatt tattatcatc aagtaccagt cccaccgagt ctaaaaatgg catcatttcc
      301 attttcagaa tcacggcgcg ttgaatctcc tctgtatttt ttaataaata cagtgagtcc
      361 tttaatgtca gggccgaacg ttcaatatct ttaaagaaat agccaacgtt tttctgaata
      421 agttcaaagc ggctatcggc acgttttacc gcgcgttccg ataccagttg atagttaatg
      481 atgaaaaaaa gaccaataaa tgacagtgct gccaataaaa ataaaattat cagccttatc
      541 tttatctttt cgtataaact cagcatgtta atacatttaa caaaataact atctgaacgc
      601 tgtgccgctg caaaaatagt cccttgctgc ggctttttaa ccaaattacc cgattctaca
      661 aaatcagcag aaagttacaa cacttgctta gggcaataat ttcctgtgag gtgattaccc
      721 tttcaagcaa tattcaaacg taactatcct ttaattttcg gatccagcgc atcgcgtaaa
      781 ccatcgccca acaaattgaa cgccagtacg gtcagaaaaa tagccagggc cggaaaaaca
      841 gcgacatgcg gcgcgataac catatccgct cgtgcctcat tgagcattgc tccccactct
      901 ggtgtcggcg gctgcgcacc gaggccgaga aatgacaggc tggcggcaga gataatcgag
      961 gtgccaatgc gcatggtgaa aaacaccacg atagacgaga cggtcccagg caagatatga
     1021 cgcaataaaa tggtcatatc gctggcacca atactgcgtg ctgactcaat aaaggtttgc
     1081 tgtttcaaca ccagcgtgtt gccgcgcacc agtcgggcaa acgcggggat ggaaaaaatg
     1141 gcgactgcga taatcacgtt agcaatgccg cttcccaaca ccgcaacaac agcgatcgcc
     1201 agtaaaatac ccgggaaggc aaacagcaca tcgcaaatgc gcatgatcag ccgatcccac
     1261 cagccttcat aatatccagc caacaagccc agcaacgtcc cgatcgccgc accgataaac
     1321 acggcaaaca cgcccgccgc cagcgagatt tgcgcaccaa ccaggacacg gctgaaaatg
     1381 tcgcgcccca gtgaatcgac gccaaaccag tgctgcaaag aaggtccgtt attcagattg
     1441 tcataatcaa aataattttc ggcgtcatag ggagcgatcc agcgtgcaaa aatggccacc
     1501 acaatcaata aaataacgaa taatgcggcg gtcatcgcca tatgctgacg gcgaaatcgt
     1561 cgccagaatt catgccacgg tgtacgcacc tggtcaggtt tgaccagtgg catggcgttt
     1621 aacaccgcct gacgtcgcca gttaaatagt cgcatcctta cttgtacctg atagccgggt
     1681 taatggcggc gtaaagcaca tccaccacta agttgataag aataaattcc agcgagaaaa
     1741 gcagaatttc cgcctgaatc accgggtaat cgcgcatttc tacggagtca acgagtaagc
     1801 gtccaagtcc gggccagttg aaaactttct ccaccacgat agaaccaccg agcaaaaagc
     1861 caaactgtaa gcccatcatg gtcactaccg ggatcatcgc gttacgtagc ccgtgtttga
     1921 ggacaaccca ggtttcgctc acccctttcg ccctcgcggt acgcatataa tcttcgctta
     1981 aaacatcgac aaacgacgcg cgggtaaagc gcgccatcac ggctgccact gccgcgccga
     2041 gcgtcaggga gggtaaaatg tagtgctgcc aactgtctgc tcccacggta ggcagccagc
     2101 ccagttcaac ggagaatacc tgaattaaaa gcatccccag cgcaaatgcc ggaaacgaga
     2161 tccccgacac cgcaatggtc atactcaatc gatccggcca acgattacgc cagacggcgg
     2221 cgataattcc cgccgccata ccaaatatca ccgcccagac catactggtt atggtcagcc
     2281 acagcgttgg cataaagcgg ctggcaatct catcggcaac cggacgacgc gacaccatcg
     2341 acaggccaaa atccccctgc acagcattgc tgatatagtg ccagaactgg tgatacagcg
     2401 gctgatccaa ccccagctgc tgacgcacca gttctataac ctgcgcatca gcttcgagcc
     2461 cggcaatcaa tcgcgccgga tcgccgggca gcatatgcac aaataaaaac accagcaccg
     2521 agacgataaa cagcgtcgga atcaacccca gtaagcgttt gataacgtaa ttaagcattc
     2581 cactccctgc gttgcttatt gcaaatccgc gtcttcaaag ctgaagccgg tgtctggcat
     2641 gatccaaaaa ccggtcaggt ttttactgtg tgccgacacc agtttttcta ccaccagcgg
     2701 gatccacggc gactcttgcc agatgatatc ctgcgccgcc ttatacaagc gggtcttttc
     2761 cgccggatca ttagttttca gtgcctgagc caggaagtcg tccacctgtt tattgctgta
     2821 aaatgcggta ttaaacagcg tcggtggcca gttttgcgag gcgaacagtg gcgatagcgc
     2881 ccagtccgct tcgccggttg aagccgacca gccagtgtag aacatccgca cgccgctctc
     2941 tttttgccct ttaccttcaa cttctgccgc ccgctgtccg gcatccatcg cggtcacctg
     3001 ggctttaatc ccgacctgcg ctaactgctg ctgggtaaat tgcagcactt tctgcgcggt
     3061 gctgtggtta tgtgacgacc acagcgtggt actgaaaccg ttgggatatc ccgcctcttt
     3121 cagtaattcg cgcgctttca ctggatcgta aggccacggt ttataacttt gcgcgtaggc
     3181 gatacttggc ggtaccacac cagtagctgg cgttgcatag cccgcaaagg caactttcac
     3241 cagcgccgga cggttaatgg cgtaattcag cgcctcacgg accttcgggt tatcgaacgg
     3301 cttttgcgtc acgttcatac tgatataacg ctgcataatt gacggactgg ccatcaactc
     3361 gatatttttg tttttctcca gcagtgtggc ctgctcgtag ggaatgggga aagcaaactg
     3421 cgcttcaccg gtttgcagca ttgccgcgcg ggtgttgtta tccgccaccg gacgccaggt
     3481 tatgctgtcc agtttgggca atcctggctg ccagtaaccc gcgaattttt tcaccttcac
     3541 aaaatcggtc tgattccagg tatccagttc atacggtccg gttcccaccg gatgaaaacc
     3601 aatctccttg ccatattttt ccagcgctgc cggtgaaatc atcgcggtcg ccggatgggc
     3661 aagaatatta ataaacgctg agaacggctg tttgagggta atctttaccg ttgtcggatc
     3721 gatcgcctcc gttttggcaa tattcttata caggttatag cgtttaagat gattcgccgg
     3781 atcgctggcc cggtccagat tcgcttttac cgccactgcg ttgaaatcgg tgccatcctg
     3841 gaatttaatg ccttcccgca atttcacggt gtaagtaatg ccgtcatcgg aaacggtata
     3901 actctccgcc agcacgtttt tcagtttcat ctctttatcc agaccgaaca gcccctggta
     3961 aaacgatttc gctacggcct gagataacgt gtcattcgcg tcatacggat cgagcgtggt
     4021 gaaattcgat cctaccgcca ccaccacatc tttggcagcg aatgcacaag atgccatcaa
     4081 cgctgtcgca atgcccagcg ccactaaccc actacggtgt acagctcttg ccattttatt
     4141 ctccgcctga atgttatcta cgcatgaatg cgtattctga ttgtggttgt gcgacgtaat
     4201 gccccggccc gacgcattgc aacgagacgg ctgccacctc ttcgccacgc agatgaatat
     4261 tgctgggaag atcgtccgac agcagtacac gctgcggtcg ttgtcgggac ggttcagcga
     4321 ccggaactgc cgccagtaat ttacgcgtat aaggatgctg cgggttttcg aagaccgcgc
     4381 gccgtggacc aatttcaaca atttgcccga gatacatcac cgccacacga tgactaatcc
     4441 gctctaccac ggccatatcg tgggagataa acagatacgc aatgccgaaa tcacgctgga
     4501 gatcgagcaa caagttgata atctgcccgc gaatagaaac atccagcgcc gaaacggctt
     4561 cgtcggcaat gatcactttt ggattcaatg ccaacgcgcg agcaatgcag atgcgctggc
     4621 gctgaccgcc ggaaaactca tgcgggtaac gccaggcatg ttcaggtaac aggcccacgc
     4681 gctccagcaa ccacgcaacg cgtgcagccg cgtctttacc tggcaataaa ccgtgtacac
     4741 gcagcggttc gataatcgaa tcaccgatgg tctgacgtgg gtccagcgaa gcgtaagggt
     4801 cctgaaaaat aaactgaata tcccggcgca atgcctgaag tttgccgggt gacaaggtat
     4861 cgattctctg accgttaaag ataatttcgc cgccctgcga ttcgaccagg cgcagcaacg
     4921 cccgcccggt agtggattta ccgctgccag actcgcccac cagcgatagc gtttcgccag
     4981 gccagagatc aaaactgact ttctcaacgg catgcacttc ccgcgttacg cgattcaaca
     5041 aaccgctgcg caaagggaaa cgggtgacaa gattacgcac tcgtaaaaca ggttcgccat
     5101 ccaccaccgt tttctgctcg atgggggggg cctgtttcgc tggatgttca agcgatatca
     5161 acgggaaacg tcggggataa tccaaccctt tcatcgcacc aagttgcgga acagcagcta
     5221 acagcgcacg ggtgtaagga tgttgcggtg catgaaaaat ctgttcgacg gtacccgttt
     5281 ccaccgcctc gccctgatac atcaccagta cccgatcggc aatctctgcc accacgccca
     5341 tatcgtgagt gataaagata acgcccatcg acatctcttt ttgcaatact ttgattaatt
     5401 gcaggatctg cgcctgaata gtgacatcca gcgcggtggt tggctcatcg gcaatcagca
     5461 ccgccgggcg gcatgacagc gccatcgcaa tcatcactcg ctggcgcatc ccgccagaga
     5521 gttgatgcgg ataacgtgaa agaatggttt gtgcctcagg aatgcgtacc tgatccagca
     5581 tccgcttcgc ctcgaccatc gcttcttcac gactggcgtt ctgatgcaga cgaattgatt
     5641 cggcaatctg ttcacccaca gtaaataccg ggttcagcga tgtcatcggc tcctgaaata
     5701 tcatcgccat atccgcaccg cgaacatggc gcatttgtgc agcgctctgc tcgctaagtt
     5761 caatcacttc gcgactgcgc cgctgcaaca gcattttatc gcactgtact aaaccgcccg
     5821 cctgttccaa caggcgcatc aatgccaacg cagtcactga cttaccggag ccggattcgc
     5881 caacaattgc cagcgtctca ccgcgttgca gactaaaaga gagattgcgg accgcagcta
     5941 ttttctgctg gtcctgcata aaggcaatat tcagattttc aaccgccagc acattaccgg
     6001 catcaagttc atcactgtgt ggcaacggtg tccccttttt cacggtagat accggtggtt
     6061 ggcgtatcgc ctgcgtagcc ccaggcgcga tacattcctt cggtgttaaa cggtagcgcg
     6121 acattccctt catggtcgat agcgattaag ccaccgctac cgccaagcgc agggagtttt
     6181 tccattacta cccgctcgca ggcttccgcg agacttaatc cgccgtaatc cattaacgcg
     6241 gcgatgtcat atgccgccag cgcgcggatg aagacttcgc ccgtgccgat acaagaaacc
     6301 gccacactgg cgttattggc gtagcatccg gcacccacta agggactatc gccaactcgt
     6361 ccgggtaatt tattggtcat tccgcctgtg gacgtggctg ccgccaaatt gccgtctaaa
     6421 tccaacgcca cggcccccac ggtgcccatt ttctgttttt catccagtgg cgcaccgcta
     6481 tggtcgagga cggttgcccc ttccttgcgc gctgccagta gttgttcata acgcaaaggc
     6541 gtggagaaaa tctccggcga gacgcgctcc atgccacgag caaacgcaaa attttctgcc
     6601 ccttcgccaa tcatcatcac atgcgggctt tgctccatca ccagccgggc ggcaagaacc
     6661 ggattacgca gatgactaac gcccgccacc gcaccggctt tcagggtgtt accatccatc
     6721 acacaggcgt ccagttcatg ggtttcatca cgcgtaaaga cagcgccaat tccggcgtta
     6781 aacagtggac actcttccag cagacgcacc gcttccgtca ccacatccag cgcactttcg
     6841 cccgcttcca gcattttctg cccggtttca acaatggcag acaacgcctc aatgtagcgt
     6901 aattcctgtt gcagactcat ctgcgcgcgg ctaattgcac ctgcgccacc atgaattgca
     6961 atgactgctt tgcccatagt attcgtccat taagggtggc tcgccacttt tgctataaat
     7021 atcagaattt ttatgaatta ctcattcaat ttttgaatat agaaagatgg atatggcatg
     7081 taaaggcagg agccaacgct acgcccgagg tggcatacag tctgtggcgt ttttctgaca
     7141 taataggcaa attcgatttt gcctccgcag gagtgttttc atggaattta ccaccggatt
     7201 gatgtcgctc gacaccgcgc ttaatgagat gctttctcgc gtcaccccac tgaccgccca
     7261 ggaaacgctg ccactggtac agtgttttgg tcgtattctg gcgagcgatg tcgtttcgcc
     7321 gcttgatgtt ccggggtttg ataactccgc aatggacggc tacgcggtgc gtttagccga
     7381 tattgcctcc gggcaaccgc tgcccgttgc cggtaaatcc tttgccggtc agccgtatca
     7441 tggtgaatgg cctgcgggca cctgcattcg tattatgacc ggtgcgccgg tgccggaagg
     7501 ctgcgaagcg gtggtgatgc aggagcagac tgaacaaacg gacaatggcg tgcgttttac
     7561 tgctgaagtg cgtagcgggc aaaatattcg ccgtcgcggt gaagatatct ctgcaggtgc
     7621 ggttgttttc ccggcgggaa ctcgcctgac taccgcagag ctgccagtaa ttgcttcact
     7681 ggggattgcc gaagttccgg tgattcgtaa agtgcgtgta gcgctttttt ctaccggtga
     7741 tgaactccag ttgcccggtc agccgctggg cgacggccaa atctacgaca ccaaccgtct
     7801 cgccgtacac ctgatgttag aacagctggg ttgcgaggta atcaacttag ggattatccg
     7861 cgacgatccc catgccctgc gcgccgcatt tattgaagcc gacagccagg cggatgtagt
     7921 gatcagttcc ggcggtgttt cagtgggtga agcggattac accaaaacga ttcttgaaga
     7981 gctgggggag atcgccttct ggaagctggc gattaaacca ggtaaaccgt tcgcgttcgg
     8041 taaactcagc aatagctggt tctgcggcct gccgggcaac ccggtttcag cgacgctgac
     8101 cttctatcaa ctggtacagc ctttgctggc aaaactaagc ggcaacaccg ccagcggcct
     8161 gcccgcgcgc cagcgcgtac gcacagcgtc ccgcctgaaa aaaacgccag gacgtcttga
     8221 tttccagcgc ggcgtgctgc aacgcaacgc cgacggtgaa cttgaagtga cgaccaccgg
     8281 acatcagggt tcacatatat ttagctcctt tagtctcggc aactgcttta tcgtgctgga
     8341 acgcgatcgc ggcaatgtgg acgtgggcga atgggtggaa gtagaaccgt ttaacgcgtt
     8401 gttcggaggc ctgtaatggc ggaactcagc gatcaggaga tgctgcgcta caaccggcaa
     8461 atcattctgc gcggttttga ttttgacggt caggaggcgc taaaagattc tcgtgtgctg
     8521 atcgtcggcc tcggtggact cggctgcgca gcttcgctgt atctggcaag cgccggtgtc
     8581 ggcaacctga cgctgctcga cttcgacacg gtttcgctct cgaatctgca acgccagaca
     8641 ctgcacagtg atgccacggt cgggcaaccg aaggtggaat ccgcccgtga cgccctgacg
     8701 cggatcaacc cacatatcgc gattacgcca gtcaatgcac tgctagatga cgcagaactt
     8761 gcagcattga ttgctgaaca cgatctggtg ctcgactgta cggataacgt tgcggtacgt
     8821 aatcaactga acgcaggctg ttttgccgcg aaggtgccgc tggtttccgg cgcggcaatt
     8881 cgtatggaag ggcaaatcac ggtcttcact tatcaggacg gcgaaccgtg ctatcgctgc
     8941 cttagccgtt tgtttggcga gaacgcatta acctgcgtag aagcaggcgt gatggcaccg
     9001 ttgatcggcg taattggttc gttgcaggca atggaagcga tcaaactgct ggcaggttat
     9061 ggcaaaccga ccagcgggaa aatcgtcatg tacgatgcga tgacctgtca gtttcgcgaa
     9121 atgaaactga tgcgcaatcc ggggtgtgag gtgtgcgggc agtaattgcc acctccaggc
     9181 ctgataagac gcgacaagcg tcgcatcagg catttgctcc agacgccaga tgcggcgtga
     9241 acgccttatc tggcctacgg ttcgggcaca gatttccaag cgcggcaggc atcggtgcac
     9301 tccgccacgt catcagcaat ttatgaggat gcagaacgtc cggttaaatc gacgttctgc
     9361 caaacgctcc ctgccagtcc tgctcaaact tcgccacagc ggcttccacc gccggatagc
     9421 taatcatctg ttgtgccaca tccagtggca gagtaattga ttcacatcct gccagtaagc
     9481 agtccagcgc ctgacgcggg gttttgaaac tcgctgccag cactttcgcc tgcggcgcat
     9541 gcattttcaa taactggtgt aagtcggtca cagtctgaat gccgctaccg ccctgagcat
     9601 caatacgatt aacgtaaggc gcaacatatt ccgcacctgc cagcgccgac agcaaccctt
     9661 gtgctgcgcc atataccgcg gttcccagcg ttggaatccc ttccgctttt aacatcttaa
     9721 tagctgccag cccctcggcg gtcaccggaa ctttcaccac gatatccgca ataatagaac
     9781 gcaatttacg tgcgtcattc accatccctt cagcagtggt agccattacc tgggcaaaca
     9841 gacgcccctg accgcccatc gcttcatgaa gttgcggaag cacaacatcc agcggttttt
     9901 tacccgcggc gataatgctt gggttagtgg tcacacccgc cagcggaaaa atacgtgaca
     9961 gcgccttcac cgcaacaacg tctgaagtat ccagatacag ttccataacc atcctcaaaa
    10021 tgtacttttt gtctgaatgt agactatcac gggaaaactc tctgaacagt tgacgcacat
    10081 caatctaact ttcattcgaa agtattttaa tgtttgaacg aaacagagag gcaactatga
    10141 ttttcaatat tcagcgctac tcgacccatg atggccccgg tatccgcacg gtcgtatttc
    10201 ttaaaggctg ttcgctgggc tgccgctggt gtcagaaccc ggaaagccgc gcccgcacgc
    10261 aggatctgct gtatgacgca cgactgtgtc tggaaggctg cgagctgtgc gctaaggccg
    10321 cgccggaagt gattgagcgc gcgctgaatg gtttgcttat tcatcgggaa aagttaaccc
    10381 cggagcatct gacggcgtta accgactgct gtccgacaca ggcattaacc gtgtgtggtg
    10441 aagtgaaaag cgttgaggag atcatgacga ccgttctgcg cgataaaccg ttttacgatc
    10501 gcagcggcgg cggtttaacg ctttcgggtg gtgagccctt tatgcagccg gaaatggcga
    10561 tggcgctact gcaagccagc cacgaggcag gcattcatac tgcggtagaa acctgtctgc
    10621 atgtgccgtg gaaatatatc gccccttctc tgccctatat cgatctgttt cttgccgatt
    10681 taaaacacgt tgccgacgcg ccgtttaaac agtggaccga cggtaacgcc gccagagtgc
    10741 tggataacct gaaaaaactc gccgcagcgg gcaaaaaaat cattatccgc gtgccgctga
    10801 ttcagggctt taatgccgac gaaacctctg taaaagctat taccgatttt gccgccgacg
    10861 agctgcacgt tggcgaaatt catttcctgc cctaccacac gctaggcatc aacaaatatc
    10921 acttacttaa tctgccctat gacgccccgg aaaaaccgct tgatgcgcca gaactgctcg
    10981 actttgccca gcagtatgcc tgccagaaag ggttaaccgc gaccttacga ggataacaac
    11041 catgaccaca ctgaaactgg acacgctcag cgaccgcatt aaagcgcaca aaaatgcgct
    11101 ggtgcatatt gtgaaaccgc cagtctgtac cgagcgcgcg cagcactata ccgagatgta
    11161 tcaacaacat ctcgataagc cgatcccggt acgtcgcgcg ctggcactgg cgcatcacct
    11221 ggcgaatcgc accatctgga tcaaacacga tgagttgatc attggcaacc aggcaagcga
    11281 agttcgcgcc gcgccgatct tcccggaata tactgtctcg tggatcgaaa aagagattga
    11341 tgatctggca gatcgtcccg gtgctggctt tgcggtgagc gaagagaaca aacgcgttct
    11401 gcatgaagtg tgcccgtggt ggcgcggtca gaccgtacag gatcgctgct acggcatgtt
    11461 taccgatgag caaaaaggtc tgctggcgac cggaatcatt aaagcggaag gcaatatgac
    11521 ctccggcgat gcgcacctgg cggtgaattt cccgctgctg ctggaaaaag gtcttgatgg
    11581 tctgcgcgag aaagtagcgg aacgtcgctc gcgcatcaat ctgacggtac tggaagattt
    11641 gcacggcgaa cagttcctga aagcgattga tatcgtgctg gtggcagtca gcgaacacat
    11701 tgaacgtttt gcagccctgg cgcgtgaaat ggccgcgacc gaaacccgcg aaagccgtcg
    11761 cgatgaactg ctggcgatag cagaaaactg cgatcttatc gcccaccagc cgccgcagac
    11821 tttctggcag gcgctgcaac tgtgttactt catccaattg atattgcaga tcgaatctaa
    11881 cggtcactcg gtatcgtttg gtcgtatgga ccagtatctc tacccgtact atcgccgcga
    11941 cgttgaactc aaccagacac tggatcgcga acacgccatc gaattgctgc ataactgctg
    12001 gctgaaactg ctggaagtga acaagatccg ctccggctca cactcaaaag cctctgcggg
    12061 aagtcctctg tatcagaacg tcaccattgg cggacaaact ctggttgatg gtcaaccaat
    12121 ggacgcggtg aatccactct cttacgctat cctcgaatcc tgcggtcgcc tgcgttccac
    12181 tcagcctaac ctcagcgtgc gttaccatgc aggaatgagt aatgatttcc tcgacgcctg
    12241 cgtacaggtg atccgttgcg gcttcgggat gccggcgttc aacaacgacg aaatcgtgat
    12301 cccggaattt attaaactcg gtattgaacc gcaggacgct tacgactacg cagcgattgg
    12361 ttgtatcgaa accgccgtcg gtggcaaatg gggctatcgc tgtaccggca tgagctttat
    12421 caacttcgcc cgcgtgatgc tggcggcgct ggaaggcggg cgtgatgcca ccagcggcaa
    12481 agtgttcctg ccacaagaaa aagcgttgtc ggcaggcaac tttaacaatt tcgatgaagt
    12541 aatggacgcg tgggatacgc aaatccgtta ttacacgcgt aaatcaatcg aaatcgaata
    12601 tgtcgtcgac accatgctgg aagagaacgt gcacgatatt ctctgctcgg cgctggtgga
    12661 tgactgtatt gagcgagcga aaagtatcaa gcaaggcggc gcgaaatatg actgggtttc
    12721 tggcctgcag gttggcattg ccaacctcgg caacagcctg gcggcagtga agaaactggt
    12781 atttgaacag ggcgcgattg gtcagcaaca gctggctgcc gcactggcgg atgacttcga
    12841 tggcctgact catgagcaac tgcgtcagcg gctgattaac ggtgcgccga agtacggcaa
    12901 cgacgatgat accgtcgata cgctgctggc tcgcgcttat cagacctata tcgacgaact
    12961 gaaacagtac cataacccgc gctacggtcg tggtccggtt ggcggcaact attacgcggg
    13021 tacctcgtct atctccgcta acgtaccgtt tggcgcgcaa actatggcaa caccggacgg
    13081 gcgtaaagcc cacaccccgc tggcagaagg cgcaagcccg gcctccggta ctgaccatct
    13141 tggccctact gcggtcattg gctcagtggg taaactgcct acggcagcga ttctcggcgg
    13201 cgtgttgctc aaccagaaac tgaatccggc aacgctggag aacgaatctg acaagcagaa
    13261 actgatgatc ctgctgcgta ccttctttga agtgcataaa ggctggcata ttcagtacaa
    13321 catcgtttcc cgcgaaacgc tgctggaggc gaaaaaacat ccggatcagt accgcgatct
    13381 ggtagtgcgt gtcgcgggct attccgcctt cttcaccgcg ctctctccag acgcacagga
    13441 cgatatcatc gcccgtactg aacatatgct gtaatctcct cggcccggcg gcgtcgtcgc
    13501 cgggctaaat cattcacatc atcaatttca tccttacttt cattcgaaat ataatttgtg
    13561 ctctgcgtca cattgttatt acactgttac ggtttcattt tcgagccagg agcacaccta
    13621 tgagcgtaaa agttatcgtc acagacatgg acggtacttt tcttaacgac gccaaaacgt
    13681 acaaccaacc acgttttatg gcgcaatatc aggaactgaa aaagcgcggc attaagttcg
    13741 ttgtcgccag cggtaatcag tattaccagc ttatttcgtt ctttcctgag ctaaaggatg
    13801 agatctcttt tgtcgcggaa aacggcgcac tggtttacga acatggcaag caactgttcc
    13861 acggcgaact gacccgacat gaatcgcgga ttgttattgg cgagttgcta aaagataagc
    13921 aactcaattt tgtcgcctgc ggtctgcaaa gtgcatatgt cagcgaaaat gcccccgaag
    13981 catttgtcgc actgatggca aaacactacc atcgcctgaa agctgtaaaa gattatcagg
    14041 agattgacga cgtactgttc aagttttcgc tcaacctgcc agatgaacaa atcccgttag
    14101 tgatcgacaa actgcacata gcgctcaatg gcattatgaa acccgtcacc agtggttttg
    14161 gctttatcga cctgattatt cccggtctac ataaagcaaa cggtatttcg cggttactga
    14221 aacgctggga tctgtcaccg caaaatgtgg tagcgattgg cgacagcggt aacgatgcgg
    14281 agatgctgaa aatggcgcgt tattcctttg cgatgggcaa tgctgcggaa aacattaaac
    14341 aaatcgcccg ttacgctacc gatgataata atcatgaagg cgcgctgaat gtgattcagg
    14401 cggtgctgga taacacatcc ccttttaaca gctgacctct ctaccggagc cgtgcgctcc
    14461 ggttttccct tctttttaca tcctgtacat cacatcaaat cattaaactt gcattaactt
    14521 atttataact tatattgtcg catgcaaatc agaatggttt cgaatgaaag ttaatgagag
    14581 gtggttatgg cttctacttt taccagcgac acattgcctg ccgatcacaa agcagctatc
    14641 cgtcagatga agcacgcgct gcgggcgcag cttggcgacg tccagcagat ctttaatcag
    14701 ctaagcgatg acattgccac gcgagtggct gaaatcaacg cactcaaagc acagggcgat
    14761 gccgtctggc cggtgctgtc ttatgccgat atcaaagcag gtcatgttac tgcagagcag
    14821 cgcgaacaga ttaaacgtcg cggttgtgcg gtgataaaag gccatttccc ccgcgaacaa
    14881 gcgctaggct gggatcagtc gatgctggac tatctggacc gcaaccgctt tgacgaggtc
    14941 tacaaaggcc ccggcgataa tttcttcggg acgctcagcg cttcacgtcc cgagatttac
    15001 cccatctact ggtcgcaggc gcaaatgcag gcccgccaga gtgaagaaat ggcgaatgcg
    15061 cagtcgtttc tcaatcgtct gtggacattt gaaagtgatg gaaagcaatg gtttaacccg
    15121 gatgtgagcg tcatctaccc tgaccgtatc cgccgccgtc cgcccggaac gacctccaaa
    15181 ggtcttggag cgcataccga ctccggggcg ctggaacgct ggctgcttcc agcgtatcag
    15241 cgcgttttcg ccaacgtctt taatggcaat ctggcgcaat atgatccctg gcatgcggca
    15301 catcgtacgg aagttgaaga gtacacggtg gacaacacca ccaaatgttc cgtgtttcgg
    15361 acattccaag gctggacagc gctctctaat atgctgcctg gtcaggggct gctgcacgtt
    15421 gtgcccattc ctgaagccat ggcgtacgta ctgttacgtc cgctgcttga tgatgtgccg
    15481 gaggatgaac tgtgcggcgt agcgcccgga agagtgttgc cggtatcaga gcaatggcat
    15541 ccactgttaa ttgaggcgtt aaccagcatt ccaaaactcg aggccggaga ctccgtctgg
    15601 tggcactgcg acgtcatcca ttccgttgcc cccgttgaaa atcaacaggg ttggggcaac
    15661 gtgatgtaca ttcctgcggc accaatgtgc gagaaaaatc ttgcctacgc gcacaaggtg
    15721 aaggccgcac tggaaaaagg cgcatcgccg ggcgacttcc cgcgcgagga ctatgaaaca
    15781 aactgggaag gacgctttac gcttgccgac ctcaacattc acggtaagcg agcgttggga
    15841 atcgacagct gagtgtcgta cagcccttcc cgttccacgg cggtgttccc ttttcctggg
    15901 cgaacccgcc gcactgactc ccgcaccgcc aatcgtccgt gcagcagcag cgagcccacc
    15961 ggagcatccg ggcgaatcag ccgctgttgc aacatttgca ccgcttcatt gcctagctca
    16021 tcgcgtggca catgcacagc cgtcagggga acatcctcga ttgccgcaag gttaaagccg
    16081 tcgatactca tcaccgagac atcctgtggt acgcgcagac cacgacgatg tagcgcattt
    16141 accgttcccg ccgccataaa atccccgccg accagaaacg ccgtcggcaa cgtcttgcca
    16201 ttggcggcat ccagccacgc tccgaccttt tcttccgctt ctttagcgct aaaactgctg
    16261 atattcagca aatgacggtc atggtgaaag gccagattat gctgacgcca tgattcttta
    16321 atccctgcaa accgctgctc catagtgtag cggcgcaggc acatcacatt catcacctcc
    16381 cggtgaccca tttcaaacag atagcgggcg gcgaacgcgc caattaaccg atgatccggc
    16441 gcaatcgccg ggaggcgcat tcgttcatca cggcagttga tcagcacaca aggcttagcg
    16501 agatcggcgg ccagatcatg gatgtgcgga tcgtcaatcc ccagcaggat cgctgcctgc
    16561 gtatctgctt cattcatccg cgagagaaag agcgcgggat cgctgtcaaa ctcctccagc
    16621 gcgcagtaac gcagccgcac ttcatgcggc gataacgctt tgctgatgct ctggatcaca
    16681 cggtaataaa agatatcaga tcgttggtca aaagcgcatt gcggcgcgaa aataatcagg
    16741 ttgttcagca acatacgacc tgctgccatg ccatccatca cgcccaactc acgcgcgcag
    16801 gccagcacct gttcgcgagc gcgtgcactg gtattcgctt tgccagccag cacccgcgaa
    16861 accgtactcg gcgatagccc ggtacgtgcg gcgatatcgg cgattttcag cttcttgtcc
    16921 attttgtgat ctgcctctaa ttcggaaatg acagaatttt catgagcaaa aaagcccatt
    16981 ctgtctggca ttaaatccat ttcagtaatg tatatcagtc actcatggaa aaacgtgcac
    17041 aaaacccgct ttctttcgct tttagggtct cttactgtga attggtagaa caacttccat
    17101 acaaaaataa tggaaaaacg aacaataaag actttattta tcaaaaagtt aaaagtggtg
    17161 taacaactcc attctgacgt tgccgcacaa accctatacc attctgtgga gtgcatatga
    17221 gtcagggtat caataacgat atgactgcca gtaaaccgcg ccatatcgtc aaaaatctgc
    17281 gttggtggat actggtcctt tttttgctcg gtgttactgt caactacatc acgcgtaact
    17341 cgctgggtat tctcgccccc gaactgaaaa gcagcctagg gattaccacc gaacaatatt
    17401 cctggattgt aggtgctttc cagctcgcct ataccctatt ccaaccgttg tgcggatggc
    17461 tgattgacgt cattggcctc aaactgggct ttatgatctg cgcgggactg tgggcactgg
    17521 cctgtctgat gcatgcgggg gcgggaaact gggtacatct ggcgctgttg cgcttcttta
    17581 tgggcggtgc ggaagccgcg gcaaccccag caaacgccaa aaccatcggt gaatggtttc
    17641 caaaatcaga acgcccgatc gccgcaggct gggcaggcgt cggtttttcc atcggtgcca
    17701 tgctggcccc accgattatc tattttgccc acgcttcatt cggctggcag ggtgcattta
    17761 tgttcactgg catcctggcg atgctgtggg tggtgctgtg gtgggcgttc tatcacagcc
    17821 cggataagca tcctaacctc agcaaagccg agctggactt tattcgccag gataacgaag
    17881 cgcccccggt caagttaccc tttctgaccg cactgaaaac cgtgggcaaa aataaacgct
    17941 tttacggtat cgctatcccg gcctttatgg cagaaccggc gtgggcggtg atgagtttct
    18001 gggtgcctct atacctgtcc aaaacctatg gcatggacct gaagcagatc gcattattcg
    18061 cctggttgcc gttcctcgcc gctgatcttg gcagtattgc cagcggctat ctgaccaaac
    18121 tttacacccg cctgtttggg ctgagtcgtg tgaactccgt tgtcgcatca tcggtcagtg
    18181 gcgcattctt gatggtttcg ctggcggtga tggcctttac caataacccg tatatcgcca
    18241 tcgtccttat ctccatcggc ggcttcggtc accagatcat ctcctgcatg ctgagcgcac
    18301 tggtggtgga atccttcgac aaaggtcaga tggccactgt taacggcatg cgcggttctg
    18361 ccgcgtggat cgcaagtttc ctgttctccc tgattatcgg ggttaccgct gacaaaatcg
    18421 gctttaaccc cctgtttatc gccatgggct tctttgacct gattggcgct ttcttcctgg
    18481 tcacctttat tgctgaacgt cgcgcaaaac gcgcctgaga gtggatgtaa acatgaaaac
    18541 gctgaaaaac tggaccctgc acaaacagtc tgcccaccac gttgaactgc tggtcgatgg
    18601 ccagcatatc ctgtgcctgt acgtgctgga agaaaatctg ttccgcgtgc tgttaaaacg
    18661 caaaggcgag ctggcgctag atcgcacctg gagcattgct ccggcgcagg atgtgccatg
    18721 ggaagggcgc agccgcgaag atctcagcgg ctttagcctg cccgcgtgga cagtgatcca
    18781 gcacgacgac accgtggtta tcgccaccga gtcactgcgt gcaaccgttc accagccgct
    18841 gtggctggag tggcactatc gtaacaacga aggcgaatgg ctgccgttgg tgaatgaccg
    18901 cccgaccagc gcttatctgc tgaatgccca cggcgatggc gtggcacact atcagagccg
    18961 tcgtaaagat gaacgctttt atggcctcgg agataaagcc ggcgatctgc aacgcaacgg
    19021 taaacgttac gagatgcgta acctcgacgc gatgggctac aacgccgtta gtactgaccc
    19081 gctgtacaaa cacattccat tcaccatcac ccgccgcgat gacattagct tcggcctgtt
    19141 ttatgacaac ctcagcagct gctggctgga tctgggcaac gagatcgaca actaccacac
    19201 cgcctatcgc cgctggcagg cggaagcggg tgatatcgac tactacctgt ttaccggcga
    19261 acgcgtactt gatatcacca aagccttcgt acgcctgacc ggaaaaaccc tgttcggacc
    19321 gaaatggagc ctgggctaca gcggttcaac tatgcactac accgatgcgc cggacgcaca
    19381 aaatcagttg atgaacttta tccgcctgtg tgaagaacat gcaattccct gcgattcgtt
    19441 ccagctctct tccggttata cctcaattaa cggcaaacgc tatgtgttca actggaacta
    19501 cgacaaagtg ccgcagccga aagtgatgag ccaggcattt cacaatgctg gtttgcacct
    19561 ggccgccaat atcaagccgt gcctgttgca ggatcaccca cgctataacg aagtggcaga
    19621 aagaggactg ttcatccgtg attcagaaac agatgaacca gaacgttcca gcttctggga
    19681 tgacgaaggt tctaacctcg actttactaa tccgcagacc atccagtggt ggcaggaagg
    19741 cgtgaccaca cagcttctgg agatgggtat cgactccacc tggaacgaca acaacgaatt
    19801 cgaagtatgg gacggtgaag cacgctgtca cggctttggt cagacgatcg cgatcaaaca
    19861 tattcgccca gtgatgccac tgctgatgat gcgagcctcg atggaagccc agcagcgttt
    19921 cgctccggaa aaacgtccgt acttgatctc ccgctccggc tgcgcgggaa tgcagcgcta
    19981 cgtgcagacc tggagtggcg ataaccgcac tagctgggac actctgcgct acaacacccg
    20041 catgggtctg gggatgagcc tctccgggct gtataacctc ggtcacgacg tcggcggttt
    20101 ctccggtgat aagccggacc cggaactgtt tgtacgctgg gtacaaaacg gcgtgatgca
    20161 tccgcgcttt acgattcact catggaacga tgaccatacc gtaaacgaac cgtggatgta
    20221 tccgggcgtc actccggcga tccgcagcgc tattgagttg cgctatcgcc tgctgcccta
    20281 cttttatacc ctgatgtggc aggcatacgc cgatgatgaa ccaatgctac gtccgacttt
    20341 ccttgaccac gagcacgacg ttcagacttt tgaagagtgt gatgacttcc tgcttggtcg
    20401 cgatattttg gttgccagcg tcgtagagca aggcgagcgc cagcgccgtg tatggctgcc
    20461 agataacgaa actggctggt acgatttcta caacggcgaa tggttcagtg gcggtcagtg
    20521 gatcaccctc gacgcaccgc tggagaaact gccgctgatg gttcgtgccg gggctggtct
    20581 accgctcagc aaacgcatca cctatgtgag cgcagaacaa gacgataccc gcgaactgaa
    20641 actgttcccg ctgaaaggtg tgggcacaac gtccggcctg ctgtttgaag acgatggcga
    20701 aagctggggc taccaaactg gtaatgcgct ttgggtggaa tgggaaatgg tttgcgatgg
    20761 ggcgaccatc aacctgaaag tcaacgcgcg cggggattat cgtcctgcat ggagtgcact
    20821 gaaagtgtca ttaccattgg aagaaaaacg cacgctgctg gtcaatggtg ttgaagggag
    20881 tgaatggatg cgttaaatca ttaccggggc cgggaggcga ctgcacctta cccggtccag
    20941 ccatcgaatc ctttacctca gtatttcacg ctgcaggaag gcagcaaacc tgtaaacccc
    21001 gggagcttaa ataagtaagt gaccggggtg aacaggtgaa gccaatgcat ctgccgcatg
    21061 aaagacggcg gggaattaag cgtcgatccc tttactacgc aggtaatctt cgtaattacc
    21121 gctaaagtcg atcgcgcgtt ccggggtgat ttccagaatg cgggtcgcca gggagcttac
    21181 gaactcacgg tcgtgtgaaa caaagatcag cgtgccctga tacagttcca gcgccatgtt
    21241 cagtgactcg atggattcca tatccaggtg gttggtcggt tcgtccatga tcagaatgtt
    21301 cggcttctgc atcattaact taccaaacag catccgccct ttttccccac cggaaagcac
    21361 tttagctggc tttttgatgt cgtcctggct gaacagcaaa cgaccgagaa tgctgcgtac
    21421 cgcctgctcg tcatcgcctt cctgcttcca ctgactcatc cattcgaaca cggtcagatc
    21481 attttcaaac tcatattcgt ggtcctgggc gtagtaacca atgcgcgcgt tttcagacca
    21541 ttttacggtg ccgctgtccg gttgcagatc gcccaccagc gttttcagca gcgttgattt
    21601 accgacgccg ttggtaccca gtaccgcaag tttttcaccg acctccagca gcaggttgag
    21661 atttttaaac agtggaccgt tatcaaaccc tttggtcaga ccttccactt ccagcgcgtt
    21721 acggaacagt ttcttatcct gttcaaaacg gatgaacggg ttctgacggc tggaggcttt
    21781 cacctcttcc agtttgattt tatcaatctg gcgcgcgcgc gaagttgcct ggcgagattt
    21841 cgaggcgttg gcgctaaagc ggctaacgaa agattgcaac tcagcaatct gcgctttctt
    21901 cttggcgtta tcggccagca gacgttcacg cgcctgggtc gccgccgtca tgtactcatc
    21961 gtagttaccc ggataaacgc gcagctcgcc gtaatccaga tccgccatgt gggtacagac
    22021 catgttaagg aagtgacggt cgtgcgagat gatgatcatg gtgctgtcac gctcgttcag
    22081 cacctgttcc agccagcgaa tggtgtcgat gtcgaggttg ttggtcggtt cgtcgagcag
    22141 gagaatatcc ggatcagcaa acagcgcctg cgccagaagc acacgcagtt tccagccagg
    22201 agcaacttca ctcatcgggc cgtagtgctg ttccactgga attcccacgc caagcaacag
    22261 ttcaccggcg cgagcttccg cagagtaacc gtccatttcg ccgtatttaa cttccagatc
    22321 ggccacttta tagccgtctt cctcactcat ttccggcaaa gcatagatgc ggtcacgctc
    22381 ctgcttcact tcccacaact ctttatgccc catgatcacc gtatccagca cagtgaactc
    22441 ttcaaaggca aactgatcct gacgcagctt accaatgcgc tcgttgggat cgagggaaac
    22501 gttacccaaa gtcggctcta agtcgccgcc gaggatcttc ataaaggtgg atttaccact
    22561 accgttcgcg ccaatcaggc cgtaacggtt gccgccgccg aatttgacgg aaatgttttc
    22621 aaacaacggc ttactgccga actgcatggt gacgttactg gaaactaaca caggcgtatc
    22681 ctgaaaagag atatgacaaa ccgcgcatta tgccataagt ctgaaagaag atcctgctcc
    22741 tcgcttaagg tctacactca agccaaaggc aaaaaatgtg atttcgtaca catctgattt
    22801 cactgtgagc tggaatgaac ttataatgcg cttccaatac tctaatattc tcaacccaat
    22861 ggcctgccag gcacaaaatc tcgcttaaca tgaatatgaa attgaaaaca ttattcgcag
    22921 cggccttcgc tgttgtcggc ttttgcagta ccgcctctgc ggtaacttat cctctgccaa
    22981 ccgacgggag tcgcctggtt ggtcagaatc aggtgatcac cattcctgaa ggtaacactc
    23041 agccgctgga gtattttgcc gcggagtacc agatggggct ttccaatatg atggaagcga
    23101 acccgggtgt ggataccttc ctgccgaaag gcggtactgt actgaacatt ccgcagcagc
    23161 tgatcctgcc ggataccgtt catgaaggca tcgtcattaa cagtgcagag atgcgtctgt
    23221 attactatcc gaaagggacc aacaccgtta tcgtgctgcc gatcggcatt ggtcagttag
    23281 gcaaagatac gcctatcaac tggaccacca aagttgagcg taagaaagca ggcccgacct
    23341 ggacgccgac cgccaaaatg cacgcagagt accgcgctgc gggcgaaccg cttccggctg
    23401 tcgttccggc aggtccggat aacccgatgg ggctgtatgc actctacatc ggtcgcctgt
    23461 acgctatcca tggcaccaac gccaacttcg gtatcggcct gcgtgtaagt catggttgtg
    23521 tgcgtctgcg taacgaagac atcaaattcc tgttcgagaa agtaccggtc ggtacccgcg
    23581 tacagtttat tgatgagccg gtaaaagcga ccaccgagcc agacggcagc cgttatattg
    23641 aagtccataa cccgctgtct accaccgaag cccagtttga aggtcaggaa attgtgccaa
    23701 ttaccctgac gaagagcgtg caaacagtga ccggtcagcc agatgttgac caggttgttc
    23761 ttgatgaagc gattaaaaac cgctccggga tgccggttcg tctgaattaa tcttcaaaac
    23821 ttaaagcaaa aggcggactc ataatccgcc ttttttattt gccagaccct agttggccgg
    23881 gagtataact aacaaaacat atcccaccaa cgccgcccac aacagcatcg gtattgaata
    23941 gagatggaaa cgccaccaga tgcggcgatc gttcgccata cgtagcgcaa tcaaatttgc
    24001 cagcgatccg ggtaataacc caaagccacc tacgtttacc gcccatacca gtaataaaga
    24061 cggcggcaca tagttcagca acaatatggt actcggcacg ttactgatca cctgcgataa
    24121 accgattgcc gttaaccata acccgggttc agatagatga ctcacgttac ccaacacgcc
    24181 ttgcaacgct ggaagctggg tcagtaaatg gacgtcgata aacatcgcca taaacaccag
    24241 cagcagcgtc cagtccacac tgagcaccac gcgacgtgcc agcagcgcaa agcctgccgc
    24301 cacaatcacc agtccccaca gctcttgttt gaactccagc gccgtcagaa agacgatata
    24361 cagccccaga caactccaca ccagccgcgg tttccactcc ggtgtttgca cccccgtatg
    24421 gtattgcagc gcctttccag ggaaacaaca ccagcacagg agcaggagcg tcagcatcat
    24481 tgcgccagcc agcggtgcca tttgggcaat aaatccggca aacgaaagac cagaacgtcc
    24541 ccagataaga atattttgcg ggttgccaat tggcgtcagt agcgaaccag cgttgactgc
    24601 cagcgcctca aaaataatca gccgattaac cgggatctca cacagtcttt ttagcgtgat
    24661 agtcagcgga acaacaataa acagcgcgac atcgttggtc agaaaggtag aaagcagcgc
    24721 cgccgccagc accataaaca tcgccagccg acgctccgta gcaaagcggc gcaccatttt
    24781 gcgccccagc acatcaaaat aaccgcttaa ctccacacct ttggtcagca gcatcaggcc
    24841 gcttaaggtg atgatggtgt gccagtcgat agcagcaggc caggatttcg gtgcaaaggg
    24901 cacgaaaaag cttaatccga taccaacaag aattaataac tgaaaaaaac gatcgccttg
    24961 cagcgtgcgt aaaaaaggca ggctcatttg gcaccgtgtt tctgggtaaa caaacgaaaa
    25021 gcgtcgagcg tctcttcact aacatggtgc tccatgcctt ccgcgtcgcg acgggcgatt
    25081 tccggactga cgcccaacac caacaagaaa ttttcgacta tctgatgtcg ctcgcggctt
    25141 tcctgcgcca gcttctctcc ttctgccgtt aaaaacacgc cacgccaggg gatcatttca
    25201 atcagcccca tggttgccag ccgcttaagc attttagcca ccgtcggttg cgaaactccc
    25261 agacgagcag ccatgtccac ctgacgagct tcccccactt ccctgatcaa gtcagaaatc
    25321 agctcaacgt aatcatcaat aagctcgcgc cgatgcgcct ctctgacctg gcggaaccct
    25381 tcaacgtgct cttccacgtt cactaactgc gtcacttttt ttgctgttgg cgtacctgcg
    25441 cgacgactca ttgtgcttcc tcattatggt gacgcataca tagcgttctc atttcgtgcg
    25501 cacattgtaa accagagttg cgaaggtaca aaaaattaac gttttagcaa tagctacata
    25561 atatagcctg tgctatatct gtatgtaatg caatcatccc tcaaggatcg acgggattag
    25621 caagtcagga ggtcttatga atgagttcaa gaagtgtatg cgcgtgttta gtcattctcc
    25681 ctttaaagta cggttaatgc tgctctctat gttgtgcgat atggtcaaca acaaaccgca
    25741 gcaagataaa ccttccgata aatagcggcg tcgcggtacg ccgcttcact cctgctttta
    25801 tgcaggcata acgcgttttg gtctgaaaaa ccccactttt tgtcggattt gcaatcccct
    25861 tcgcaaaaga tttgttcgtc agtagttgac ctgaacggcg gctcgctcta tcttcttgca
    25921 gccctgcgta tattgcggct cgcggatgcg gacccctttc ccctcttcac gcactcttgc
    25981 aggtattgac ccttgacgcc agggtaagca catggcgttt gttacgatag tggcatatga
    26041 atttaaccct caaagaatcg cttgttaccc gtagccgggt atttagcccg tggactgcgt
    26101 tctacttttt acagtcgcta ttaattaacc tcggcttagg ttaccccttc agtttgctct
    26161 acaccgctgc gtttacggct attttgcttt tgctatggcg aacattgcct cgcgtacaaa
    26221 aagttctggt cggtgtcagt tcgctggtgg cggcttgtta tttccctttt gctcaggcct
    26281 acggcgcgcc taatttcaat acattgctgg cattgcactc caccaatatg gaagagtcga
    26341 ccgaaatcct gacgattttt ccgtggtaca gctacctggt cggcttattt atttttgcgc
    26401 tcggcgtaat agcaatcagg cgaaaaaaag agaatgaaaa agcgcgctgg aataccttcg
    26461 acagcctgtg tctggtattc agtgtggcga cattttttgt tgctcccgtg caaaacctgg
    26521 cctggggtgg cgtatttaaa ctgaaagata ctggctatcc ggtatttcgt tttgctaagg
    26581 atgtcatcgt caataataac gaggtgattg aagagcaaga acggatggca aaactttccg
    26641 gaatgaaaga tacctggacg gtcactgccg ttaagccgaa gtatcagacc tatgtggtgg
    26701 tgatcggcga aagcgcgcgt cgcgatgccc tcggtgcctt tggcggtcac tgggacaata
    26761 ccccgtttgc cagcagcgtt aacggtttga tatttgctga ctacattgcc gccagtggct
    26821 ccacgcagaa atcacttggc ttaacgctca atcgcgtagt cgatggcaaa ccacagtttc
    26881 aggataactt tgtcaccctg gcaaatcgcg cgggcttcca gacctggtgg ttttccaacc
    26941 agggccaaat cggcgaatac gatactgcta tcgccagcat cgccaaacgt gcagatgaag
    27001 tgtacttcct gaaagaaggt aattttgaag cagataaaaa cacgaaagac gaagcgttac
    27061 tggatatgac cgctcaagtg ctggcgcaag agcactcgca accgcagctg attgttctgc
    27121 atctgatggg ctcgcatccg caggcctgcg acaggacaca aggcaaatac gaaacctttg
    27181 tgcaatcgaa agaaacgtcg tgctatctct ataccatgac gcaaacggac gatttactgc
    27241 gcaagctgta cgatcagtta cgcaacagcg gcagcagctt ctctctggtt tacttttctg
    27301 accacggtct ggcctttaaa gagcgcggca aagacgtgca ataccttgcc catgatgata
    27361 aatatcagca aaatttccag gtgcctttta tggtcatttc cagcgacgat aaagcgcatc
    27421 gggtgattaa agcccgccgc tcagccaatg acttcttagg ctttttctcg cagtggacgg
    27481 gaattaaagc gaaggaaata aatatcaaat acccgtttat atctgagaag aaagccgggc
    27541 cgatatacat caccaacttc cagttacaga aggtggatta caaccatctc ggaaccgata
    27601 ttttcgaccc gaaaccttaa aacaaaaaat ccgccccgag aggcggattt tttatatcac
    27661 caaagtgatt agaagcggta accaacaccg gcaatccagg tgcctacgtc aacgctacga
    27721 atacggctct gctcgtaaga gaagtccaga gcaacgtttt ccatcgggtt gaactgcaga
    27781 ccagcaccgt aggagaaacc gtagtcgctg gtgtcgtgtt tgtaggtcgg gtattcagtg
    27841 gtctggaatt taccataacc cacccccact acaccgtaga tgcttgccca gtcgttaatg
    27901 cggtaagccg gaccagcagt gatgccgtag tactggtttt tgttgtagtc accagagctt
    27961 gcagtacggc ttttctcggt gtaagtgaaa gaaccgatca cacccagcgg gctgttgtct
    28021 tcttcatagc ggtatttcag gttgaaaccg cccattttgt tcatttggcc ctgagcgtcg
    28081 ctctgtgcgt aaccgccagt tacggtagaa gtcgcagcta cggaagtacc tgcggtgaaa
    28141 gccagaactg cggccagtgc tgaaagacat gcaatttttt tcataaccac ctcaaatgtg
    28201 attcaaataa gtcctaagtt ttaaatatat caaaaattaa tgggaaactc ttcgcgattt
    28261 gtgatgtcta acgggccatt tcatgtaaca gaacgtttcc atacaccgct atccatctaa
    28321 atttaaatca ctttttcaga gaactgcgta agtattacgc atgttttccc tgtcattcat
    28381 ccagattatt cctaatcacc agactaatga ttccatcaat cctggcgcat tttagtcaaa
    28441 acgggggaaa attttttcaa caaatgctca accagcattg ggtatatcca gtacactcca
    28501 cgctttactt aagtctagat atttgtggga gaaaggatgc ctggttcatt acgtaaaatg
    28561 ccggtctggt taccaatagt catattgctc gttgccatgg cgtctattca gggtggagcc
    28621 tcgttagcta agtcactttt tcctctggtg ggcgcaccgg gtgtcactgc gctgcgtctg
    28681 gcattaggaa cgctgatcct catcgcgttc tttaagccat ggcgactgcg ctttgccaaa
    28741 gagcaacggt taccgctgtt gttttacggc gtttcgctgg gtgggatgaa ttatcttttt
    28801 tatctttcta ttcagacagt accgctgggt attgcggtgg cgctggagtt caccggacca
    28861 ctggcggtgg cgctgttctc ttctcgtcgc ccggtagatt tcgtctgggt tgtgctggcg
    28921 gttcttggtc tgtggttcct gctaccgctg gggcaagacg tttcccatgt cgatttaacc
    28981 ggctgtgcgc tggcactggg ggccggggct tgttgggcta tttacatttt aagtgggcaa
    29041 cgcgcaggag cggaacatgg ccctgcgacg gtggcaattg gttcgttgat tgcagcgtta
    29101 attttcgtgc caattggagc gcttcaggct ggtgaagcac tctggcactg gtcggttatt
    29161 ccattgggtc tggctgtcgc tattctctcg accgctctgc cttattcgct ggaaatgatt
    29221 gccctcaccc gtttgccaac acggacattt ggtacgctga tgagcatgga accggcgctg
    29281 gctgccgttt ccgggatgat tttcctcgga gaaacactga cacccataca gctgctggcg
    29341 ctcggcgcta tcatcgccgc ttcaatgggg tctacgctga cagtacgcaa agagagcaaa
    29401 ataaaagaat tagacattaa ttaaatttac atttctgcat ggttatgcat aaccatgcag
    29461 aatttctcgc tacttttc
//
LOCUS       DAZPEP010000038        29391 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZPEP010000038 DAZPEP010000000
VERSION     DAZPEP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.038"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            66..1505
                     /gene="norV"
                     /locus_tag="ACHUT9_001659"
     CDS             66..1505
                     /gene="norV"
                     /locus_tag="ACHUT9_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFR1348162.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            1502..2635
                     /gene="norW"
                     /locus_tag="ACHUT9_001660"
     CDS             1502..2635
                     /gene="norW"
                     /locus_tag="ACHUT9_001660"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFR1348163.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2763..5015)
                     /gene="hypF"
                     /locus_tag="ACHUT9_001661"
     CDS             complement(2763..5015)
                     /gene="hypF"
                     /locus_tag="ACHUT9_001661"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFR1348164.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5168..5695)
                     /gene="hydN"
                     /locus_tag="ACHUT9_001662"
     CDS             complement(5168..5695)
                     /gene="hydN"
                     /locus_tag="ACHUT9_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFR1348165.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5843..6853)
                     /gene="ascG"
                     /locus_tag="ACHUT9_001663"
     CDS             complement(5843..6853)
                     /gene="ascG"
                     /locus_tag="ACHUT9_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFR1348166.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7113..8570
                     /gene="ascF"
                     /locus_tag="ACHUT9_001664"
     CDS             7113..8570
                     /gene="ascF"
                     /locus_tag="ACHUT9_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFR1348167.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            8579..10003
                     /gene="ascB"
                     /locus_tag="ACHUT9_001665"
     CDS             8579..10003
                     /gene="ascB"
                     /locus_tag="ACHUT9_001665"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFR1348168.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10117..10536)
                     /locus_tag="ACHUT9_001666"
     CDS             complement(10117..10536)
                     /locus_tag="ACHUT9_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1348169.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(10533..10844)
                     /locus_tag="ACHUT9_001667"
     CDS             complement(10533..10844)
                     /locus_tag="ACHUT9_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFR1348170.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(11006..11779)
                     /locus_tag="ACHUT9_001668"
     CDS             complement(11006..11779)
                     /locus_tag="ACHUT9_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348171.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            complement(11804..12274)
                     /gene="hycI"
                     /locus_tag="ACHUT9_001669"
     CDS             complement(11804..12274)
                     /gene="hycI"
                     /locus_tag="ACHUT9_001669"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFR1348172.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(12267..12677)
                     /gene="hycH"
                     /locus_tag="ACHUT9_001670"
     CDS             complement(12267..12677)
                     /gene="hycH"
                     /locus_tag="ACHUT9_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFR1348173.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(12674..13441)
                     /gene="hycG"
                     /locus_tag="ACHUT9_001671"
     CDS             complement(12674..13441)
                     /gene="hycG"
                     /locus_tag="ACHUT9_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFR1348174.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(13441..13983)
                     /gene="hycF"
                     /locus_tag="ACHUT9_001672"
     CDS             complement(13441..13983)
                     /gene="hycF"
                     /locus_tag="ACHUT9_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFR1348175.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(13993..15690)
                     /gene="hycE"
                     /locus_tag="ACHUT9_001673"
     CDS             complement(13993..15690)
                     /gene="hycE"
                     /locus_tag="ACHUT9_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFR1348176.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            complement(15708..16631)
                     /gene="hycD"
                     /locus_tag="ACHUT9_001674"
     CDS             complement(15708..16631)
                     /gene="hycD"
                     /locus_tag="ACHUT9_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFR1348177.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            complement(16634..18460)
                     /gene="hycC"
                     /locus_tag="ACHUT9_001675"
     CDS             complement(16634..18460)
                     /gene="hycC"
                     /locus_tag="ACHUT9_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFR1348178.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(18457..19068)
                     /gene="hycB"
                     /locus_tag="ACHUT9_001676"
     CDS             complement(18457..19068)
                     /gene="hycB"
                     /locus_tag="ACHUT9_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFR1348179.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(19193..19654)
                     /gene="hycA"
                     /locus_tag="ACHUT9_001677"
     CDS             complement(19193..19654)
                     /gene="hycA"
                     /locus_tag="ACHUT9_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFR1348180.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            19866..20216
                     /gene="hypA"
                     /locus_tag="ACHUT9_001678"
     CDS             19866..20216
                     /gene="hypA"
                     /locus_tag="ACHUT9_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFR1348181.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            20220..21092
                     /gene="hypB"
                     /locus_tag="ACHUT9_001679"
     CDS             20220..21092
                     /gene="hypB"
                     /locus_tag="ACHUT9_001679"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFR1348182.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            21083..21355
                     /gene="hypC"
                     /locus_tag="ACHUT9_001680"
     CDS             21083..21355
                     /gene="hypC"
                     /locus_tag="ACHUT9_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFR1348183.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            21355..22476
                     /gene="hypD"
                     /locus_tag="ACHUT9_001681"
     CDS             21355..22476
                     /gene="hypD"
                     /locus_tag="ACHUT9_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFR1348184.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            22473..23483
                     /gene="hypE"
                     /locus_tag="ACHUT9_001682"
     CDS             22473..23483
                     /gene="hypE"
                     /locus_tag="ACHUT9_001682"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFR1348185.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            23557..25634
                     /gene="flhA"
                     /locus_tag="ACHUT9_001683"
                     /pseudo
     CDS             23557..25634
                     /gene="flhA"
                     /locus_tag="ACHUT9_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(25671..26024)
                     /locus_tag="ACHUT9_001684"
     CDS             complement(25671..26024)
                     /locus_tag="ACHUT9_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFR1348186.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(26101..26235)
                     /locus_tag="ACHUT9_001685"
     CDS             complement(26101..26235)
                     /locus_tag="ACHUT9_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348187.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            26311..28872
                     /gene="mutS"
                     /locus_tag="ACHUT9_001686"
     CDS             26311..28872
                     /gene="mutS"
                     /locus_tag="ACHUT9_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFR1348188.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            28978..29391
                     /locus_tag="ACHUT9_001687"
                     /pseudo
     CDS             28978..29391
                     /locus_tag="ACHUT9_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT         6996 a         8043 c         7813 g         6539 t
ORIGIN      
        1 actggcacgt aatctgcaat tagcaagaca tctttttaga acacgctgaa taaattgagg
       61 ttgctatgtc tattgtggtg aaaaataaca ttcattgggt tggtcaacgt gactgggaag
      121 tgcgtgattt tcacggtacg gaatataaaa cgctacgcgg cagcagttac aacagctacc
      181 tcatccgcga agaaaaaaac gtgctgatcg acaccgtcga ccataaattc agccgcgaat
      241 ttgtgcagaa cctgcgtaat gaaatcgatc tggcggatat cgattacatc gtgattaacc
      301 atgcagaaga ggaccacgct ggggcgctga ccgaactgat ggcgcaaatt cccgatacgc
      361 cgatttactg tactgccaac gctatcgact cgataaatgg tcatcatcat catccggagt
      421 ggaattttaa tgtggtgaaa actggtgaca cgctggatat cggcaacggc aaacagctca
      481 tttttgtcga aacgccaatg ctgcactggc cggacagcat gatgacttac ctgacaggcg
      541 acgcggtgct gttcagtaac gatgctttcg gtcagcacta ctgcgacgag catctgttca
      601 acgatgaagt ggatcagacg gagcttttcg agcagtgcca gcgttactac gccaatatcc
      661 tgacgccgtt cagccgcctg gtaacaccga aaattaccga gatcctgggc tttaacttac
      721 cagtcgatat gatagcgacc tctcacggcg tggtatggcg cgataacccg acgcaaattg
      781 tcgagctgta cctgaaatgg gcggctgatt atcaggaaga cagaatcacc attttctacg
      841 acaccatgtc gaataacacc cgcatgatgg ctgacgctat cgcccagggg attgcggaaa
      901 ccgacccacg cgtggcggtg aaaattttca acgtcgcccg aagcgataaa aacgaaatcc
      961 tgaccaatgt cttccgctca aaaggcgtgc tggtcggcac ttcgacgatg aataacgtga
     1021 tgatgccgaa aatcgccggt ctggtggagg agatgaccgg attacgcttc cgtaacaaac
     1081 gcgccagtgc tttcggctct cacggctgga gcggcggtgc ggtggatcgt ctttccacgc
     1141 gcctgcagga tgcgggtttc gaaatgtcgc ttagcctgaa agccaaatgg cgaccagacc
     1201 aggacgctct ggagttatgc cgtgaacacg gtcgcgaaat cgcccgtcag tgggcgctcg
     1261 cgccgctgcc gcagagcacg gtgaatacgg tggttaaaga agaaacctct gccaccacga
     1321 agtccgacct cggcccacgg atgcagtgca gcgtctgcca gtggatttac gatccggcaa
     1381 aaggcgagcc aatgcaggac gttgcgccag gaacgccgtg gagtgaagtt ccggataact
     1441 tcctctgccc ggaatgctct ctcggcaaag acgtctttga cgaactggca tcggaggcaa
     1501 aatgagcaac ggcattgtga tcattggttc gggcttcgcc gcccgccaac tggtgaaaaa
     1561 tattcgcaaa caggacgcca gtattccatt aaccctgatc gccgccgaca gcatggatga
     1621 gtacaacaaa ccggacctca gccatgttat cagccagggg caacgtgccg acgaccttac
     1681 ccgccagacg gcgggtgaat ttgccgggca gtttaatctg cacctgtttc cgcacacctg
     1741 ggtgacggat atcgatgccg aagcccatgt ggtgaaaagc cagaataatc agtggcaata
     1801 cgacaagctg gtgctggcaa ctggtgccag tgcctttgtc ccgccagtgc ctgggcgtga
     1861 gttaatgctg acgttaaata gtcagcaaga gtatcgcgcc tgtgaaacgc aactgcggga
     1921 tgcccgacgc gtgttgattg tcggcggtgg tttgattggt agcgaactgg cgatggattt
     1981 ttgtcgtgca ggcaaagcgg tcacgctaat cgacaacgct gccagtattc tggcgtcatt
     2041 aatgccacca gaagtaagca gccgcttgca gcatcggttg acggagatgg gcgttcatct
     2101 gctgttgaaa tctcagttgc aggggctgga aaaaacggat tctggcattc tggcaacgct
     2161 ggaccaccag cgtagtatcg aagtggatgc ggtaattgcc gccaccggac tgcgcccgga
     2221 aacagccctg gcacgacgcg ccgggctgac gattaatcgc ggcgtttgcg tcgatagtta
     2281 tctgcaaacc agtaatgacg atatttatgc gctgggcgat tgcgcggaaa tcaacggtca
     2341 ggtactgcca ttcctgcagc cgattcaact tagcgcgatg gtgctggcaa aaaatcttct
     2401 cggcaataac acgccgctga aactaccggc gatgctggtg aaaatcaaaa cgccggaatt
     2461 accgctgcat ctggcaggcg aaacccagcg tcaggattta cgctggcaaa ttaataccga
     2521 acgccaggga atggtggctc gcggtgttga cgatgctgac cagcttcgcg cctttgtggt
     2581 cagtgaggat cggatgaaag aggcatttgg attgttgaaa acattgccga tgtaggtggg
     2641 ctactgtgcc taaaatgtcg gatgcgacgc tggcgcgtct tatccgacct acggggacgc
     2701 atgtgtaggc cggataaggc gtttacgccg catccggcaa tggtgtccaa atgcaacacg
     2761 ttttatccgt tctggacttc acccgctaac caacgcgccg cagcaataac cccctgcccc
     2821 agagacaaac cgccatcacc cgccggtaaa ctctgtggaa agagcaatgt gaaatcagcg
     2881 agataatgcg ccagacgtgc acgcagcaaa cggttatgaa taaccccgcc gctaaatacc
     2941 agcgtagtga taccacgcat cgtggcctgc tcacgcatca acgcggcaaa accctgcgcc
     3001 agcgcatcat gaaacgccca cgcgcgttga ttaaccggtg cctgccagtt cagccactgc
     3061 tgccagaaag cggcgagatc cagttgattg tccaccagcg gcatcgtcac cggatgcgtc
     3121 actccgtggc atgaggctgc gagcgcctcc agagcacaag ccgcttcacc ttcataactt
     3181 aacgtggctg gcgcacagcc cagtgccgcc gccactgcat cgaacaaccg cccacacgat
     3241 gacgccagcg gcgcgttaat tccacgctca atggcccgcg ccagcacgct ccagttttgc
     3301 tgttgcacac ttgctgtttc agggtaattc tgccactccg gcacaaagcg caggcactgc
     3361 gccagcaggt ttcgccacgg ctgcttcgct gccaaatcgc cacccggaag cgccactgca
     3421 ggcaagccgc ccaggtgctc acattcgcga tagttcaccc gcaggcactc gccgccccac
     3481 aaagcgccgt tctcccccat accgataccg tcgagcgtca aagcaatgac atcaccgcca
     3541 tccaacggcc actgatgctc tgccagacac gccgctgcat gggcatgatg atgcagtacc
     3601 gtttgcgtcg gcagattcat ttcacgcgcc cactggctgg agacatagcc cggatgcgcg
     3661 tcatgcacaa cgtattgcgg ggtaaaatcg tagatgtttt gcatcaggcg taacgcttcg
     3721 cgccactgca tctggatgcc atcgtcactt aaatcgccca gatgctgact caacaccgct
     3781 tgttcaccgc gcaccaggca gaaggtattt ttcagatccg cgccgagaca cagcacaggc
     3841 ggaacatttt taaagcccgg aggcaaagcc agcgcatccg gcacataccc ccgcgaacgg
     3901 cgcagcattt cgccgctttc gcgcaccacc gaatcatcca tccgctgcac gatgtcgcgg
     3961 ttatgtatca agaatccgtc ggcaatgccc tgcaaatccg ccagcgcctg ttcgttgctg
     4021 atagctggtg gtttaccgct caggttgccg gaggtcatca ccagcgggca ttgcagttcc
     4081 tgtaacagca aatgctggag cgggttcgca ggcaacatta ccccgacttc gttaaggtca
     4141 ggggcgatat catcacaaag ctcaggaacg tattttttat ccaccagcac aatcggcgcg
     4201 gcgggcgtgg taagcaactg gcgcgcagcg tctggtaaac cgtcagccac tggcaacatg
     4261 accgccagcg gtttcgccgg gcgatgtttg cgcgcccgaa gtgtcgccac cgcgttactg
     4321 ttacgtgcat cgcaggcaag atgaaatccg ccaatccctt tgatggcgac aatgttgccc
     4381 atttttaact gtgcgatagc cgcctgtaat gccgcctctt gttctgcatg ttcaccatga
     4441 cttacccatt caagatgtgg gccacactcc gggcaggcca ccggctgggc gtggaagcga
     4501 cgatcgagcg ggtcacggta ctctttgtca caagccggac acaacggaaa cgccgccatc
     4561 acggtaaacg ggcggtcgta aggcatggcg cgaataatgg tgaaacgcgg gccgcagtgg
     4621 gtacagttga taaacggata acgataacgc cgttcgcctg gggtattcat ttcggcaagg
     4681 caagcagggc aagtagcggc atccgggaca atttgcgtat tcatggcacc tcccgcgctc
     4741 tggcggatgg tgaactcagt gggcagttgt gaccagatgt acggctcacg ctcgacgcta
     4801 tcaatacgcg ccagcggcgg gcagtgctga tgcaattgaa caagtaacgt ttccgggtct
     4861 tcccgcagcc ggacttctac gccatcgccg tcattacaga catcgccgtg aagatttaat
     4921 tgctgtgcca gctgccagac aaacggacga aaaccgacgc cctgcacttt gccacgaata
     4981 cgcagttgga caccgcaaga tgtgtttttt gccattgagt tattcccgcc atcatgaatt
     5041 gcgtaacccg tcctgccgga cacgactgcg tcgtatccgg cagtctcagg tcggcgatac
     5101 cgccagctcc gtattctacg aatatttccg ggaattcctt tgatgccaga acagttctgt
     5161 aagattttta gaacatcagc gccgtacggc ggcgtttttc tgcactcagt tgttcaagtt
     5221 tattacgatc gacacaaatc agcgcatgag tcgggcaagc cgccatacac gccgggccgt
     5281 cttcacgatg gttgcacagg tcacatttat tggcttcggc tttgtcagcc cgtacattca
     5341 gccccgcgcc gctgttgcga atcaccggac gtaccaccac ttccatcgcg ccatacgggc
     5401 aagccacaac gcaggttttg caaccaatgc aacgttcctg catcacatga acaaaccctt
     5461 tatcacggct gatagcacca ttcgggcaga cgttagcgca cggtgcatct tcacactgac
     5521 ggcaaactgt cgccgtggaa atgttcacac ctttaatgac atggatacgc ggtaaaaatg
     5581 tttccggggt cagcgatgca cagtcctgat tttcctgatg agaaaccacg cacgccactt
     5641 cacaggtacg gcaaccaata catttactcg cgtcagcaat gatgaaacgg ttcatcaaat
     5701 tctccagcaa tgacagttaa tgcgccgata cattcacaaa tcatgccagt tttaattcac
     5761 tgttatttaa ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg acactagtga
     5821 cgatgacatg tgatgacaat gtttatcgcg aaagagcaat gagtgagtcg cggcggatca
     5881 gttttccgct gaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc
     5941 gtccaataat ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa
     6001 cgatataggg ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg
     6061 ctacgccgcg ctcgtgcaac gctttcatcg cacctatcgc catatcgtcg ttactggcaa
     6121 ctaacgcgct aaatttagcc ccacgttcga gcaacgtttc taccccttcg gccccgctgg
     6181 cgggcgtcca tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgcca
     6241 gcgcgtcttt atacccagca agacgttcaa tgctggtggg ggaatccatc gagccggtaa
     6301 ggaaagcaat ctcctgatgt ccggcgttta tcaactctgc cacggcatta aagctggtct
     6361 gtttatgatc gcaccagacg ctatggctgc tgtttttgcg caggcggcga ttaagcacca
     6421 ttatcggctg actgtgcgcg tcaatgatgt catcgatctc atccacgctt aaaaaacgcg
     6481 ggtaaatcat aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt
     6541 cttcggcgct gtgtttacca tctgccaata gcaactgccg ccctttctct tccgccattc
     6601 gcgcggcatg aaagagtaat tcactaaaat aaatgccgtg gtaaagcgtg ttggtcacta
     6661 ccaaccccag cgtctgagta ctcttcgccg acagattgcg cgccagcaag tttgggcggt
     6721 aaccgctctc ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat
     6781 tacctgaaag cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca
     6841 gcatcgtcgt catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt
     6901 gccgattgtc cttgcgcaat cggcgggaaa aatattcagg tgaccggttt cacaaatata
     6961 aaaaatgaac aattaactct cttgcttatt tagtgacaac tattcatgat tttgtgaaac
     7021 cggtttccta attccgtttc agcatcggca tttttccgtt acgtcgactg ataacaacta
     7081 catctaccct actgataaca ggataaaatc cgatggccaa aaattatgcg gcgctggcac
     7141 gctcggtgat agcggcactg ggcggcgttg ataacatctc ggcggtcacg cactgtatga
     7201 cgcgtttgcg cgttgttatc aaagatgacc agcttatcga cagcccgacg ttaaaaacca
     7261 tccccggcgt gctcggcgtg gtacgtagtg acaaccagtg tcaggtgatt atcggcaata
     7321 ccgtttcaca agcctttcag gaagtcgtca gcctgctgcc gggagatatg cagcccgcac
     7381 agcccgtggg taaacccaaa ctcacgctgc gtcgcattgg tgcggggatc ctcgatgcgc
     7441 tgatcggcac catgtcaccg ctgatcccgg cgattatcgg cggatcgatg gtcaaactgc
     7501 tggcgatgat cctcgagatg agcggcgtgc tgacaaaagg atcgccgacc ttaaccattc
     7561 tgaatgtgat tggtgacggt gctttcttct tcctgccgct gatggtcgcg gcatctgccg
     7621 ccatcaaatt taaaaccaat atgtcgctgg cgattgccat cgcgggtgtg ctggtacatc
     7681 cgagttttat tgaactgatg gcgaaagcgg cccagggtga acatgtcgaa tttgccctga
     7741 ttccggtcac cgcggtgaaa tacacctaca cagtgatccc ggcgctggtg atgacctggt
     7801 gcctgtcata tatcgaacgc tgggtggatc gcattacacc ggcggtgacc aaaaacttcc
     7861 tcaagccaat gctgattgtg ttgattgccg caccgctggc aatcctgctg attggcccga
     7921 ttggtatctg gatcggtagc gccatttcgg cgctggttta caccattcat ggttatctgg
     7981 gctggctttc agtcgccatt atgggcgcgc tgtggcctct gctggtaatg accgggatgc
     8041 accgcgtctt tacgccaacc atcattcaga ccattgccga aaccggcaaa gaagggatgg
     8101 tcatgccgtc agagatcggc gctaacctgt cgctgggcgg ttcatcactg gcggtggcgt
     8161 ggaaaacgaa aaacccggaa ctgcgccaga cggcgctggc tgcggcggca tcagccatta
     8221 tggcggggat ttccgaaccg gcgttatacg gcgtggcgat ccgcctgaaa cgtccgctta
     8281 tcgccagtct tatcagcggt tttatttgcg gcgcggttgc cggtatggcg gggcttgcca
     8341 gccactcaat ggcagcgcca gggttattta ccagcgtgca attcttcgat ccggcgaatc
     8401 caatgagcat cgtctgggtg ttcgcggtca tggcgctggc ggtggtgctg tcgtttatcc
     8461 tcacactgtt gctcggcttt gaggatattc ctgttgagga agcggctgcc gaggcgcgaa
     8521 agcatcagag cgtacaaccg accgtcgcca aagaagtaag tcttaattga ggatgaaaat
     8581 gtcagtattt ccagaaggtt ttttatgggg cggcgcgctt gccgccaacc agtctgaagg
     8641 tgcgttccgt gaaggtggca aagggctgac cactgtcgat atgatcccac acggcgagca
     8701 tcgaatggcg gtgaaactgg ggctggaaaa acgttttcag ttgcgcgatg acgagtttta
     8761 tcccagccat gaggcgacgg atttttatca tcgttataaa gaagatatcg ccctgatggc
     8821 agagatggga ttcaaggttt tccgtacctc aattgcctgg agccgtctct ttccgcaggg
     8881 cgatgaaatc acgcccaatc agcagggcat tgctttttat cgttctgtct ttgaagagtg
     8941 taaaaagtac ggtatcgaac cgctggtcac gttgtgccac ttcgatgtgc cgatgcatct
     9001 ggtcaccgaa tatggctcct ggcgtaaccg caagctggtg gagtttttca gccgctacgc
     9061 ccggacctgc tttgaagcat ttgatggtct ggtgaaatac tggcttacct tcaatgaaat
     9121 caacattatg ttgcatagcc cgttctccgg cgcgggtctg gtgtttgaag aaggtgaaaa
     9181 tcaggatcag gtgaaatatc aggccgcgca tcaccagctg gttgccagtg cgctagccac
     9241 caaaatcgcc catgaggtta acccgcaaaa tcaggtgggc tgtatgctgg cgggcggtaa
     9301 cttctaccct tacagttgca agccggaaga tgtctgggcg gcgctggaga aagaccggga
     9361 aaacctgttt tttatcgatg tgcaggcgcg gggcacgtat ccggcttact ctgcccgcgt
     9421 attccgcgaa aaaggggtaa ccatcgacaa agcaccgggc gatgatgaaa tcctgaaaaa
     9481 caccgtcgat tttgtctctt tcagctatta cgcctcgcgc tgcgcctcgg cggagatgaa
     9541 cgccaacaat agcagtgcgg cgaacgtagt gaaatcgctg cgtaacccgt atctgcaagt
     9601 gagcgactgg ggctggggta ttgatccact cggtctgcgt atcaccatga acatgatgta
     9661 cgaccgttat cagaagccgc tgtttctggt ggaaaacggc ctcggcgcaa aagatgaact
     9721 tgctgccaat ggcgagatta acgatgacta tcgcatcagc tatttacgcg aacatatccg
     9781 cgcaatgggc gaagcgattg cagatggcat tccgctgatg ggctacacca cctggggctg
     9841 tattgattta gtctccgcct ctacgggtga aatgagcaaa cgctacggtt ttgtctacgt
     9901 agaccgtgac gacgcaggca acggcacgct gacgcgcaca cgtaagaaat cgttctggtg
     9961 gtataaaaaa gtgattgcca gtaatgggga agatttagag taggtaacag tgccggatgc
    10021 ggcgtgaacg ccttatccgg cctacatgtc gcacgaacca ggaaggcccg ataagcgcag
    10081 cgcatcgggc aatctggggt ttggcatcag tcaaaatcaa agaaaccatt ccggcttaac
    10141 gccaaatcgg gctgaaagtg ccttaatgtg ggatatcgtc agggatcgtt gtccagacaa
    10201 aatctgactg accagggatt tagagccgat ttcgttcttc aaatccgcat aggtcagatt
    10261 atgttgatca atcagcgtgc gaagtaacgc cacgccaaca ggcattgccg caacggcttt
    10321 gtcgaactct gcaaactctt ggttgttgtt ttcatattct gcaatgcggc tcgccagaaa
    10381 gtcgataagt ggattggtgt catcttcttc aatcaaatat tcgaccagtt ccagtgcttc
    10441 acgataatca tcttctgagt cactacctcc cagaaagggg actgcattga ccagttctct
    10501 tgttgcttta acagcgcgtg cagcattagc agtcattctt tgttctctct gtagtaacgt
    10561 gtcagtttgt catactcggc gtgattagtt atgtgcttca caaagaaccg cttattaaca
    10621 aagtttatgt aggcaatcac ccttaggttg ttcccaccta catccagaac gtaccatttg
    10681 ttgcgatatt taaaattgtc gaggttggga aacgccgttc gcatctcttc tggcgtagaa
    10741 aaatccgttt ccttaataac ccgatacaat gcctgaagag ccagagcgtc gttaggatat
    10801 ttacgcgcag actcttcaaa tggcgcttta gaaatgatgt gcatttttaa gttttaactt
    10861 ccttatagta agtaaacagc cacatctgcg agttctatcc ttatgtgttc ggaaactata
    10921 gctcatcaaa tccaagttca caatatgtaa acacttgagc gcgatataat tacccccaaa
    10981 atcctcatag ccactctgca aaaagtcagt tctggttcac cccatcaaga acatccccct
    11041 ttatcaagca acgctgcgtc tgcgccagat cttccgtttg cgggtcgcgc ccggcgatgg
    11101 gtttgaggac gagtttacgg ttgggggtat aagtaaaacg cgtgagcgcg ggatagctta
    11161 acttgccaaa ccagccgtga acacgctgcc atttggcgtc ttttacctga tacagactgg
    11221 ttatccagta accggaatca aactgcatat ccagcaattg cgtgtgacca ttgccttgca
    11281 aatccagcaa atcatctacg ccagttttgc ttgcggtgta gaatcccctg ggttcaaaga
    11341 cacagggacg gccttcgcgc gtgaacgtca taagaattaa atgtgcataa ggagccagac
    11401 cattaccggt acttggtaac cagataacca gatcctgaat gccgttacga tcaagatcgg
    11461 cgcgatagag ttgccctttg cccgctatgt ttgccagcat acccacaggc acagaccagg
    11521 atttaccggc gcgatcttta ccgttcaatt gccattcact atcatcattt ggcatctttt
    11581 caagcgtcac gccagcaacg cccacttttc taaaatgcca ggtttgctcg gcatcataag
    11641 gcatggtcag gagaggtttc agcgcccgct tttgcgcgta ttcgctgtca ctcattggca
    11701 gatattcggt aaagctggtg gcctgtgcag gcagcactaa gctcaataaa aaagcgcaca
    11761 tccctgttct gatattcatt tcgctgaatt ccctgatgga gaattattct tcttccaccg
    11821 ctaactgcgc gaagccgcca ttcccttccc agccttccag tcgttgataa acggtttcta
    11881 ccgcatcttt aatcggctga gtcatcgggt agtaaaagcc gacgatatcc ggctggatgc
    11941 cgaggaaaat cacttcgcca atgtcttctt tcaactggtc gataagatag ttcagcggca
    12001 tgttatgggt agtcatcata aacatctcgg cgatatcatc cgggtcgatg atgcggattt
    12061 cgccggggtt tagccccata tcagtagcgt caacaatcag taaccgcgta gggcgcagtt
    12121 cacggatggc gacgatgtcg ttttccggtg cactaccgcc atcaatcacc acccagttac
    12181 ctttcggcgc ggcggcgcac ttttccgcca gcagcgggcc agcgccatca tcgcccatca
    12241 tgctattgcc aacacagagt aaaacgtcag tcacgcagtc tcctcaccat caaatagatg
    12301 gcgttttcct gatgaatatc atgcagcata cttaacagca ttgtactcca ttccttctgc
    12361 tgcgaagttt gcgctgcccg ggctttatcg aatgcgttgg ccagcatcgg cacatggtta
    12421 atgtcgatga cgatctcacc atatttcggc acgccttcca ttttgcggcg ggcttcactg
    12481 cccgcctcca gagtggcaat ccatgccaga tattcatccc acgggcaggt gagcgccgct
    12541 tccaggcaat cgataacccc aaggtggtga ccaatcgcca ggctgtaata gaccacctgc
    12601 tgcgcctcgg cgggcgtggc atcgttctca tcaataaatt tacggctcag ttgactgaac
    12661 accacctttt cactcatcgg atacgcgcct cttcaacaac atgattcaga tggctgacaa
    12721 tctcgttcag acgcggatcg ttttccgctt ccagccagcg tgcaacctgt tcttcgccct
    12781 gccctaactg tgtcaggaag tcatcggcaa tctgacgacc gtaacgataa cccgccaggc
    12841 ggcgtgcttc gcgatccact ttcacacgca gcggctgcac catatcgcca tgcaggattt
    12901 ccgcctgttg ttcatcctgc tcacccgggc cacgggcgtg aattttctgc tccagcaggc
    12961 cgagcgccat tgcaaagccg tacagcgtgg cggcaggcgt tggcgggcaa ccaggaatgt
    13021 agacatctac cgggacgatt ttatccgtac cgccccacac gcagtagaga tcgtggaaaa
    13081 taccaccgct gttaccgcag gcaccgtagg agatacaaat tttcgggtcc ggcgcggact
    13141 gccacgcacg cagcgcaggg gatcgcattg cacgggtgac cgcgccggta aacagtaaaa
    13201 tatccgcatg acgcggtgaa ggaacgactt taatgccgaa gcgttctgca tcaaacagcg
    13261 gcgaaagcgt ggcgaaaatt tcgatttcgc agccgttgca gccgccgcag tccacgcgat
    13321 aaacgtaggc agaacgtttg atttttttca gtaacgacgc cttcatgctg gcgatggatt
    13381 catccaccgt catcgggacc ggaatgccgt tggcgtcacg ggggcctaat aaattgctca
    13441 tcagatggcc tctttcatat ggcgagtcag ttcaatacgg tcggacggca ccaggcattt
    13501 ctggcgctta cattccgggc aagtctcaaa gctttcgcgg tggttttccg cgcggctgtc
    13561 gccgttgtgc ttaagcagcg caatggcgta gtcgatctct ttctggacgg cgaaaggacg
    13621 attgcagacg cggcagttgc acagcgcgaa gcgggactgt tgcaggaagt cttctttctt
    13681 ccacactgcc agttcgtact cttgcgacag tttgatcgcc gccgtcgggc aaacttcttc
    13741 gcagcgtcca cagaagatgc agcgcccgag attaaactcc cacgccagct caccggtggc
    13801 gaggtcagtt tcaaccgtta aggcgtttga cgggcaggca ttgacgcagg ccgcacagcc
    13861 gatgcactgc tgcgggttct gctccggctt accacggaag tttttatcaa ccgcaatcgg
    13921 ctccagcgga taagacgagg tcgccgtgcc ggttttgatg acttttttga taaaggtaaa
    13981 catggcgatt ccttatttca gcggcgagtt tttacgctca atgctgtaac gctcgagttc
    14041 tttgtacggc accactttgc tctttttctt acgcacatcg accacggtca tgcggtcggt
    14101 acaggagtag caagggtcca agctaccgat aatcagcggc gcatcggaaa cggtgttacc
    14161 gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc gcggcacggc agcgccagcg
    14221 gtacagcttc tggttgtcgc cggtcatgct ccagtggata tcgtcgccgc gcggcgcttc
    14281 ggcaaagccc agcgcgaaac ggtgcggaat gtaggtaaag ccttccacca tcagcggacc
    14341 gcccggcagg ttatccagac cgtagtcgat catgttcagc gcggtataaa cttcgttgat
    14401 acgcactttc agacgggaaa taacgtcgca gccctgctcg ctgtggactt ccattggcag
    14461 caggccataa ccgacaaacg ggtgatcggc gcgagtatcg cgggcgtgac cgctggcgcg
    14521 aaccatcggg ccaacgttac tgaaatcgcg ggccatttcc gggtccagac gaccaatgcc
    14581 gacggtgcgc tgttccatgt tcggcgtact cagcagcaca tccaccagct cctgcacttc
    14641 acgacgcatc tgttgcgcca gctggcgggt ctggatcatg tcatctttca acagatcgcg
    14701 acgaatcccg ccgatcaggt tcaggccgta ggttttacgc gcaccggtga ggatctctgc
    14761 cattttcatg gaggtttcac gcacgcggaa gaactgcata aagccggagt caaagccggt
    14821 aaagtggcag gcgagaccga ggttgagcag atgcgagtgc aggcgttcca cctccagcag
    14881 aatggcgcgg atcatctgcg cacgttctgg cacctgaata cccatcgcgt tttctaccga
    14941 cgtggtgtag gcggtgctgt gggcaaagcc gcagatcccg cacacacggt cagagaggaa
    15001 ggtcacttcg ttgtaaccca tacgggtttc cgccagtttt tccatgccgc gatgaacgta
    15061 gaacagacgg tagtcggcgt cgataatgtt ttcgccatcg acgaacagac ggaagtggcc
    15121 cggttcgtca gaagtgacgt gcagcggacc aatcggcacg acgttgtttt tcttgtcgcc
    15181 cagttcgttg atgaactcgt aggtttcagc atcggtggtc ggtgccggac gctgacgata
    15241 atccatgctg tctttacgca gcggataaag ttcatccggc agcaccagac ggcgttcatc
    15301 cggcagacca accggaatca aaccgtacat atcgcgcact tcgcgctcgc cccacaccgc
    15361 cgccggaacg cgcggcgtca cggacggata ctccggtttg ttggcatcaa cttcgacgcg
    15421 aaccgttacc cagcacttag tccccttctc catcgacagc acgtaataaa cggcgtaatg
    15481 accattcagt ttgcgttcgt cgttaccaaa aagcaccgac aaccagcccc cctgcttgta
    15541 gtagagaaac tccaccactt ccggcagata gttcaccttt acggtgacag tcagctgatc
    15601 tttggtctgc caggcgtggt ccagcacgac gcccggaaat gcctcattca gcgcggcgag
    15661 ataatgttga cctaattttt cttcagacat gctcaaactc tctttaatca cgccgccagc
    15721 aaggagacga acgctaaaaa tgcaaagcca aacccagtcc aggtaatgcg cggagtaata
    15781 tcaagacgca gacgggccat gctgttttcg aacagcgcga taaccaggac gccgacgacc
    15841 agtttgacga tggcaatcac cagcgccagc agcagcccac cggcggtgaa ggtttccatc
    15901 tgtccccacg ggataaatac gccgacgaac atctgcagca ccaccagctg tttcaggctg
    15961 ataccccatt tcatgacgcc aaagccgctg ccgctgtatt cagagagcgg gccttcctgc
    16021 agctcctgct cggcttccgc caggtcgaac ggcagtttgc ccatttcgat aaaggtggcg
    16081 aacgcacagg cacaaagcgc cagtaccagc gggatgctct ggctcagcgg ccagtgataa
    16141 acggtgtcgg tgatgttgct gatgttggtg gaaccggcaa cctgtgcggc aacccacaga
    16201 ccaagcagca gcatcggttc gaccagcacg ccaagcatcg cttcacggct cgcgccgata
    16261 gcggtaaacg ggctaccggt atccagacca gaaatggcaa agaagaaacg cgcgatggca
    16321 aagagataca gtaaggtgat taaatcaccc agttgcggca gcggagaacc gacggtcacc
    16381 accggcagcg cagtagcgat agtcagcatg acgcccacca tcacatacgg cgtcaggcgg
    16441 aacacccagc cggaggcatc cgggccaacg ctctggcggc ccagcagttt gaaaatgtca
    16501 cgatactcct gcaacacacc cggcccacga cggttatgca gacgggcgcg cgccacgcgg
    16561 gtaataccgg agagcagcgg cgcaacggca aataacacca gcgcctgaat taacggatat
    16621 aaaacactca ttctcaggct cctcgtgaaa caataatcac caccagtacc gccagttcaa
    16681 ccagcgccat ccggcggaac agcaacgcac taccttcgca ctgccagccc ggcaccagcg
    16741 acaccggatt cagccatttg cgcagtttca gcactggcgc aaacgcctgt ttaaccggca
    16801 tggcaaaacc gtgagcggta atcaccattg atttttcatg gtcgtagccg cacacccagg
    16861 ccgcaccgcg ggaacgtgac ggcaggcggt cgcctttgca aatcgccata atgatgaatg
    16921 gcagcagcgg gcaggcaatc agcagcaatg tgatcattgg ttgagaaacg gtggtgttag
    16981 caggctccag aggcagaggt acagcagcag agagcatcgg cagcagccac ggcgcagcaa
    17041 caccgccaat tacgcagcaa atcgccagtg ccactacgct tacgctcatc aggagcggcg
    17101 cacaggtggc gttttcggct tctttggtac gcggcgcgcc gaggaacgtg acgccataga
    17161 ctttcgccat acacatcacc gccagcgcac cggtaattgc cagacccaca gcgagcagcg
    17221 gccccagcag acgggcaaca aacgcgccgc tattgctcag tttgaagaag gattgataga
    17281 taacccattc cccggcaaac ccgttcagtg gcggcagcgc agccattgcc atcaacccga
    17341 ctaacatggc gatggagata accggcattt tcttgccaat accaccgagt ttttcgatat
    17401 cgcgatgacc ggtacggaac cagacgctcc ccgccccgag gaacagtacg cttttgaaca
    17461 ggctatggtt aagcagatgg tacagaccac cgaccaggcc aagagcaatc agcgccggtt
    17521 gttcgagcgc gatacccgtt acgccagcgc ccagccccag caggataatg ccgatatttt
    17581 ccagggtgtg gtaagccagc aggcgctgga tgttgtgttc catcaacgca tacagaccac
    17641 cgacgaacgc ggtgatcatg ccgagcacca gcagcgcgat cccccaccac agcggtgcat
    17701 taccgcccag cagtgacagg gttaacatgc ccagcaggcc aattttcatg actaccgtag
    17761 aaaacaacgc ggcagctggc gcagaggcgt tagcatgtgc ctgcggcacc cagccgtgca
    17821 gcggaataat cccggccagc aggccaaagc caatcacacc gagcagccag atatcggaac
    17881 cgagcggcag ctgttgcata cgcatatcca gtaggcacag atccagcgtg ccataacgct
    17941 gccacagcag ccagcaagca atcaccagca gcagagtgcc aagacgcccc agcgcaaacc
    18001 acagtttgcc ctctttgctg ttgctggtga ggaacaccgc acacaaggcc atgacttcgg
    18061 ccattaccac gaacatgccg aggttgctgg cgatgacggc gcagacggcg gcagccatca
    18121 acatattgat ctgcaagccg ttgcacttca cctgcgcgtg gcgatgccag tcaatgttgt
    18181 agaggctgac aaacagaccg cacagaccga gcgtaatcag ccaaatcgcg ttaagcggag
    18241 agatttgcac atcgtagctt accagcgaca gcgcaccgct cacgccaacc gcgccagtca
    18301 gtacagtgaa gcccgcggct gccgtataca gactaccaac cgcgccgcca attccagcta
    18361 tccagccact taacgctttt tgaaaagaaa agagaaatgc cagaacagcg gcggcgacaa
    18421 accatgccac gccgctattg atcagggaaa ttgcgctcat ttagcctctc cactttgagc
    18481 ctgctgaaac aaggtgagat cgccaaagtc cgtgttaaag gtcagctcac gcttacgttt
    18541 gctgacgcgg gcgatatcgg tgttatccac cagatgcagg gctttagtcg ggcacatccg
    18601 cacgcaggcc ggaccttgtt catcaaaact gcaaaggtca catttgacgg cgatcgcgcg
    18661 aatacccggc acccagtcaa gcagcgtact gatacgcgcc ggagcaggcg gtgccggtgg
    18721 cgctttcggg gtattggcgt ttgccggaat atccagcgga cggctgccgg aaaattcaat
    18781 tgcgccaaac gggcaggcga tgccgcacag tttgcagctt acgcacagac tttcattcaa
    18841 ctgcacggcc ccatcaatgc gggtgatggc gttaaccggg caaaccaccg cacagggtgc
    18901 atcttcacag tggtgacaga gctgcggcgc agattctttt tcattcagca tcactctcag
    18961 gcgcggcatt gattgcaggc cgtgctggcg atgcgtctct gaacaggcgg cctcacaagt
    19021 gtggcagccg atacagagcg tggagtcaac aattacaaaa cgattcacca ggcattcctc
    19081 aggtgattgt catttttgac gaaaacatgc cgatgaaatg tcattttcga cactcatcga
    19141 cacgcccatc cccaaacagg cgtaacgcct gcaaaacggg caaagcctca gctcatgctg
    19201 ccgggctttg tccctttacc agttggctta aattcaccgg cacattgttt tcaatagcgg
    19261 tgtataagct gtcgtaaacg ccagcgattt tttcgagata gtgttccaga acctgttcgc
    19321 gatcgcggat gttgaccttg ccatcaatca tgccgtcaat gctcagttgc gcctgcgcaa
    19381 tccgctgttt gtcttcgcca tcttttgttt cgacgtaata ctcgatggtg tggctgttaa
    19441 agccatccgc cagggtgacg taaatacgaa aatgttcaaa aaggacgaaa cagagttctt
    19501 tgtccggcgc gcagctcatg gcgtgatgca ggcgcgcttt cgataacgtg atcccctgaa
    19561 ccagcgaatt gcagtagatg tgccactggt cctgtaggcg acgatgccgc tgtgcgatgt
    19621 aatcggcttt ctcgcttatt tcccaaatag tcattgtcag gttacccgtt taacagagat
    19681 gccagcttta agcattttct atgccaactt ttaatttatt gttattaaat agatttttaa
    19741 gctaaagatg aatttcgtcg ccgtgtcgac gtgtcatttc gacatcatcg acattattca
    19801 ccgcagggat aatcaacact ggcacaatta ttgcttgtag ctggcaatag ttaatgggag
    19861 gcgatatgca cgaaataacc ctctgccaac gggcactgga attgatcgaa cagcaggccg
    19921 caaaacacgg cgcaaaacgc gtaactgggg tctggctcaa aattggcgca ttttcttgtg
    19981 tcgaaaccag ctctcttgcc ttttgttttg atctggtttg ccgcggcagc gtggcggaag
    20041 gttgtaaact gcacctcgaa gaacaagagg ccgaatgctg gtgtgaaaca tgccaacagt
    20101 atgtgacgct actgacccag cgcgtccgcc gctgtccaca gtgtcatggt gacatgctac
    20161 agattgtggc agacgacggt ttacagattc ggcggataga aatagaccag gagtgagcga
    20221 tgtgtacaac atgcggttgc ggtgaaggca acctgtatat cgagggtgat gaacataacc
    20281 ctcattccgc gtttcgtagc gcgccatttg ccccggcggc acgcccgaag atgaaaatca
    20341 ccggcattaa agcgcctgaa tttaccccca gccagactga agaaggcgac ctgcattacg
    20401 gtcatggcga agcgggcact cacgcgccgg gcatgagcca gcgtcggatg ctggaagtcg
    20461 aaattgacgt gctggacaaa aataaccgtc tggctgaacg caaccgcgcg cgctttgctg
    20521 cccgcaagca actggtgctc aacctggttt ccagccctgg ttccggtaaa accaccctgc
    20581 tgacggaaac cctaatgcgc ctgaaagaca gcgttccgtg cgcggttatt gaaggtgacc
    20641 agcaaaccgt gaacgatgcc gcacgcattc gcgctaccgg cacaccagcg attcaggtga
    20701 acaccggtaa aggctgccat cttgacgcac agatgattgc cgacgccgca ccgcgtctgc
    20761 cactggacga taacggtatt ctgtttatcg aaaacgttgg caacctcgta tgcccggcca
    20821 gcttcgatct cggtgaaaaa cacaaagtgg cggtgctttc cgttaccgaa ggtgaagaca
    20881 aaccgctgaa atatccgcat atgtttgccg ccgcctcgct gatgctgctc aacaaagttg
    20941 acctgttgcc gtatctcaac tttgacgttg agaagtgcat cgcctgcgcc cgcgaagtca
    21001 atccagaaat tgaaatcatc cttatttccg ccaccagcgg cgaagggatg gaccagtggc
    21061 tgaactggct ggagacacag cgatgtgcat aggcgttccc ggccagatcc gcaccattga
    21121 cggcaaccag gcaaaagtcg acgtctgcgg cattcagcgc gatgtcgatt taacgttagt
    21181 cggcagctgc gatgaaaacg gtcagccgcg cgtgggccag tgggtactgg tacacgttgg
    21241 ctttgccatg agcgtaatta atgaagccga agcacgcgac actctcgacg ccttacaaaa
    21301 catgtttgac gttgagccgg atgtcggcgc gctgttgtat ggcgaggaaa aataatgcgt
    21361 tttgttgatg aatatcgcgc gccggaacag gtgatgcagt taattgagca tctgcgcgaa
    21421 cgtgcttcac atctctctta caccgccgaa cgccctctgc ggattatgga agtgtgcggt
    21481 ggtcataccc acgccatttt taaattcggc ctcgaccagt tactgcctga aaacgttgag
    21541 tttatccacg gtccgggttg cccggtgtgc gtactgccga tgggtagaat cgacacctgc
    21601 gtggagattg ccagccatcc ggaagtcatc ttctgtacct ttggcgacgc catgcgcgtg
    21661 ccggggaaac agggatcgct gttgcaggca aaagcacgcg gtgccgatgt gcgcatcgtt
    21721 tactcgccga tggatgcgtt gaaactggcg caggagaatc caacccgtaa agtcgtattc
    21781 ttcggcttag gttttgaaac caccatgccg accaccgcca tcaccctgca acaggcgaaa
    21841 gcccgcgatg tgcagaattt ttacttcttc tgccagcata ttacgcttat cccgacgctg
    21901 cgcagtttgc tggaacagcc ggataacggt atcgacgcgt tcctcgcgcc gggccacgtt
    21961 agtatggtta tcggcactga tgcctataat tttatcgcca gcgattttca tcgtccgctg
    22021 gtggtcgctg gtttcgaacc ccttgatcta ctgcaaggcg tggtcatgct ggtggagcag
    22081 aaaatagcgg cccacagcaa ggtagagaat cagtatcgtc gggtggtacc ggatgccggt
    22141 aacctgctgg cgcaacaggc aattgctgat gtgttctgtg tcaacggcga cagcgaatgg
    22201 cgcggcttag gcgtgattga atcttctggc gtgcacctga cgccggatta tcaacgattc
    22261 gatgccgaag cacatttccg cccagcaccg cagcaggtct gcgatgaccc gcgcgcgcgt
    22321 tgtggtgagg tattaacggg caaatgtaag ccgcatcaat gcccgctgtt tggtaacacc
    22381 tgtaatcctc aaaccgcatt tggtgcactg atggtctcct ccgaaggagc gtgcgccgcg
    22441 tggtatcagt atcgtcagca ggagaatgaa gcgtgaataa tatccaactc gcccacggca
    22501 gcggcggcca ggcgatgcag caattaatca acagcctgtt tatggaagcc tttgccaacc
    22561 cgtggctggc agagcaggaa gaccaggcac gtcttgatct ggcgcagctg gtagcagaag
    22621 gcgaccgtct ggcgttctcc accgacagtt acgtcattga cccgctgttc tttcctggcg
    22681 gtaatatcgg caagctggcg atttgcggca cagccaatga cgttgcggtc agtggcgcta
    22741 ttccgcgcta tctctcctgt ggctttatcc tcgaagaagg attgccgatg gagacactga
    22801 aagccgtagt gaccagcatg gcagaaaccg cgcgcgcggc aggcattgcc atcgttactg
    22861 gcgatactaa agtggtgcag cgcggcgcgg cagataaact gtttatcaac accgctggca
    22921 tgggcgcaat tccggcgaat attcactggg gcgcacagac gctaaccgca ggcgatgtat
    22981 tactggttag cggcacgctc ggggaccacg gggcgactat ccttaacctg cgtgagcagc
    23041 tggggctgga tggcgaactg gtcagcgact gcgcggtact gacgccgctt attcagacgc
    23101 tgcgtgacat tcccggcgtg aaagcgctgc gtgatgccac ccgtggtggt gtgaacgcgg
    23161 tagttcatga gttcgcggca gcctgcggtt gtggtattga aatttcagaa gtggcactgc
    23221 cggttaaacc tgccgtgcgc ggcgtttgcg aactgctggg actggacgcc cttaactttg
    23281 ccaacgaagg caaactggtg atcgccgttg aacgcaacgc ggcagagcaa gtgctggcag
    23341 cgttacattc ccatccactg gggaaagacg cggcgctaat tggtgaagtg gtggaacgta
    23401 aaggtgttcg tcttgccggt ctgtatggtg tgaaacgaac cctcgattta ccacacgccg
    23461 aacctcttcc gcgtatatgc taataaaatt ctaaatctcc tatagttagt caatgacctt
    23521 ttgcaccgct ttgcggtgct ttcctggaag aacaaaatgt catatacacc gatgagtgat
    23581 ctcggacagc aagggttgtt cgacatcact cggacactat tgcagcagcc cgatctggcc
    23641 tcgctgtgtg aggctctttc gcaactggta aagcgttctg cgctcgccga caacgcggct
    23701 attgtgttgt ggcaagcgca gactcaacgt gcgtcttatt acgcatcgcg tgaaaaagac
    23761 accccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc cgcattttgt
    23821 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    23881 tcgcaggtgg gctatacccc aaatttggtc actattgcct gatgccactg gcggcggaag
    23941 ggcatatttt tggtggctgt gaatttattc gttatgacga tcgtccctgg agcgaaaaag
    24001 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    24061 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    24121 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    24181 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagtatc gtcttacgca
    24241 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    24301 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    24361 aagagatgct gctgatcaat ctccacgagc gggacgattt agccccctat gaacgcatgt
    24421 tgttcgacac ctagggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    24481 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    24541 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    24601 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    24661 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    24721 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    24781 gtgaaactgg cacgggtaaa gagctgattg cccgcgcgat ccataatctc agtgggcgta
    24841 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg ctggaaagcg
    24901 atctgtttgg tcatgagcgt ggggctttta ccggtgccag cgcccagcgc attggtcgtt
    24961 ttgaactggc ggataaaagc tctctgttcc tcgacgaagt gggcgatatg ccactggagt
    25021 tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca
    25081 aaatcattca gacggacgta cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg
    25141 tcgccgaccg agagttccat agcgatctct attaccgcct gaacgtattc ccgattcacc
    25201 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    25261 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ctgcgcacct
    25321 tgagcaacat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcgg
    25381 tattgctaac acgcggtaac gtgctgcagc tgtcattgcc agatattgtt ttaccggaac
    25441 ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga
    25501 ttgtgcgcgt gctgaaagaa accaacggcg tggttgccgg gcctaaaggc gctgcgcaac
    25561 gtctggggct gaaacgcacg accctgctgt cacggatgaa gcggctggga attgataaat
    25621 cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga
    25681 ggttttttcg gacgatattt ttccggcagt tctggtaccg gacgcttgtc atcgatgaga
    25741 tgacgcacgg ttaagatcgg atgacgccac agcattctcg gcccggccca acgcataatc
    25801 tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg cttacaggct
    25861 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    25921 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    25981 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc tccatactct
    26041 taaggtgtat ttatattaca acttaatttg aaaggggact atggctctga aggaggaggt
    26101 ttattcaacc gacgcaattt tgcgtacttg cttcataagc atcacgcaaa agctgcaaaa
    26161 cagcatcttt cccggaacca gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat
    26221 taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacacccc
    26281 atttaatatc agggaaccgg acataacccc atgagtgcaa tagaaaattt cgacgcccat
    26341 acgcccatga tgcagcagta tctcaggctg aaagcccagc atcccgagat cctgctgttt
    26401 taccggatgg gtgattttta tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg
    26461 ctggatattt cactgaccaa acgcggtgct tcggcgggag agccgatccc gatggcgggg
    26521 attccctacc atgcggtgga aaactatctc gccaaactgg tgaatcaggg agagtccgtt
    26581 gccatctgcg aacaaattgg cgatccggcg accagcaaag gtccggttga gcgcaaagtt
    26641 gtgcgtatcg ttacgccagg caccatcagc gatgaagccc tgttacagga gcgtcaggac
    26701 aacctgctgg cggctatctg gcaggacagc aaaggtttcg gctacgcgac gctggatatc
    26761 agctccgggc gttttcgcct gagcgaaccg gccgaccgcg aaacgatggc ggcagagctg
    26821 caacgcacta atccggcgga actgctgtat gcagaagatt tcgccgagat gtcgctgatt
    26881 gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc
    26941 cagcagttga atctgcaatt tggcacccgc gatctggtcg gttttggcgt cgagaacgcg
    27001 ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg
    27061 accctgccgc atattcgttc tatcaccatg gaacgtgagc aggacagcat cattatggat
    27121 gccgcgacac gtcgtaacct ggaaattact cagaacctgg cgggcggtgc ggaaaatacg
    27181 ctggcttctg tgctcgactg caccgtcacg ccgatgggta gtcgtatgct gaaacgctgg
    27241 ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca
    27301 ttgcaggatt tcaccgccga gttgcagccg gtactgcgtc aggtcggcga cctggaacgt
    27361 attctggcac gtctggcttt acgaactgct cgcccacgcg atctggcccg tatgcgccac
    27421 gctttccagc aactgccgga gctgcgtgcg cagttagaaa ctgtcgatag tgcaccggta
    27481 caggcgctac gtgagaagat gggcgagttt gccgagctgc gcgatctgct ggagcgagca
    27541 atcatcgaca caccgccggt gctggtacgc gacggtggtg ttatcgcatc gggctataac
    27601 gaagagctgg atgagtggcg cgcgctggct gacggcgcga ccgattatct ggagcgtctg
    27661 gaagtccgcg agcgtgaacg taccggcctg gacacgctga aagttggctt taatgcggtg
    27721 cacggctact acattcaaat cagccgtggg caaagccatc tggcacccat caactacatg
    27781 cgtcgccaga cgctgaaaaa cgccgagcgc tacatcattc cagagctaaa agagtacgaa
    27841 gataaagttc tcacctcaaa aggcaaagca ctggcactgg aaaaacagct ttatgaagag
    27901 ttgttcgacc tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg
    27961 gaactcgacg tgctggtgaa cctggcggaa cgggcctata ccctgaacta cacctgcccg
    28021 accttcattg ataaaccggg cattcgcatt accgaaggtc gccatccggt agttgaacaa
    28081 gtactgaatg agccatttat cgctaacccg ctgaatctgt cgccgcagcg ccgtatgttg
    28141 atcatcaccg gtccgaacat gggcggtaaa agtacctata tgcgccagac cgcactgatt
    28201 gcgctgatgg cctacatcgg cagctatgta ccggcacaaa aagtcgagat tggacctatc
    28261 gatcgcatct ttacccgcgt aggcgcggca gatgacctgg cttccgggcg ctcaaccttt
    28321 atggtggaga tgactgaaac cgccaatatt ttacataacg ccaccgaata cagtctggtg
    28381 ttaatggatg agatcgggcg tggaacgtcc acctacgatg gtctgtcgct ggcgtgggcg
    28441 tgcgcagaaa atctggcgaa taagattaag gcattgacgc tgtttgccac ccactatttc
    28501 gagctgaccc agctaccgga gaaaatggaa ggcgtcgcca acgtacatct cgatgcgctg
    28561 gagcacggcg acactatcgc ctttatgcac agcgtgcagg acggcgcggc gagcaaaagc
    28621 tacggtctgg cggttgctgc tctggcgggc gtaccgaaag aggttattaa gcgcgcacgg
    28681 caaaaactgc gtgagctgga aagcatttcg ccgaacgccg ccgcgacgca agtggatggt
    28741 acgcagatgt ctttgctgtc agtgccagaa gaaacttcgc ctgcggtcga agcactggaa
    28801 aatctcgacc cggattcact gactccgcgt caggcgctgg agtggattta tcgcttgaag
    28861 agtctggtgt aataacaatt cccgatagtc ttttgctatc gggaatatta acgacaactg
    28921 acgaatcaaa taaaaatacc ctgtataata ggaaagctta ttttacaggg taaaaccatg
    28981 ccatctacac gctatcaaaa aatcaatgcc catcactatc gccatatatg ggtcgttggt
    29041 gatattcatg gtgaatatca gttattacaa tcccgttaac atcaactctc tttttaccct
    29101 gaaaccgact tacttatttc cacaggcgat aatattgatc gtgggccgaa gagtcttaac
    29161 gtgctgcgcc tgctaaacca accctggttt acgtcggtta aaggcaacca cgaagcgatg
    29221 gcgttggatg cattcgaaac tggcgatgga aatatgtggc ttgccagcgg tggtgactgg
    29281 tttttcgatt taaatgattc agagcaacaa gaggcaatag atctgttgct gaaattccat
    29341 caccttccac atattattga aatcattaat gacaacataa aatatgccat c
//
LOCUS       DAZPEP010000039        28894 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZPEP010000039 DAZPEP010000000
VERSION     DAZPEP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28894
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.039"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHUT9_001688"
     CDS             complement(<1..1262)
                     /gene="ipaHb"
                     /locus_tag="ACHUT9_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaHb"
                     /protein_id="HFR1348189.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRAFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFTV
                     AADATESCEDRVALTWNNLRKTLLVHQAS"
     gene            1753..2310
                     /gene="pinE"
                     /locus_tag="ACHUT9_001689"
                     /pseudo
     CDS             1753..2310
                     /gene="pinE"
                     /locus_tag="ACHUT9_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2649..3497)
                     /locus_tag="ACHUT9_001690"
     CDS             complement(2649..3497)
                     /locus_tag="ACHUT9_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFR1348190.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHUT9_001691"
     CDS             3706..4341
                     /gene="yfhb"
                     /locus_tag="ACHUT9_001691"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFR1348191.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4399..4902
                     /gene="tadA"
                     /locus_tag="ACHUT9_001692"
     CDS             4399..4902
                     /gene="tadA"
                     /locus_tag="ACHUT9_001692"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFR1348192.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQPSTD"
     gene            complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHUT9_001693"
     CDS             complement(4899..6455)
                     /gene="mltF"
                     /locus_tag="ACHUT9_001693"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFR1348193.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDCTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6713..10600
                     /gene="purL"
                     /locus_tag="ACHUT9_001694"
                     /gene_synonym="purI"
     CDS             6713..10600
                     /gene="purL"
                     /locus_tag="ACHUT9_001694"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFR1348194.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            11158..12585
                     /gene="qseE"
                     /locus_tag="ACHUT9_001695"
     CDS             11158..12585
                     /gene="qseE"
                     /locus_tag="ACHUT9_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFR1348195.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12750..13463
                     /gene="qseG"
                     /locus_tag="ACHUT9_001696"
     CDS             12750..13463
                     /gene="qseG"
                     /locus_tag="ACHUT9_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFR1348196.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13453..14787
                     /gene="glrR"
                     /locus_tag="ACHUT9_001697"
     CDS             13453..14787
                     /gene="glrR"
                     /locus_tag="ACHUT9_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFR1348197.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14848..15186
                     /gene="glnB"
                     /locus_tag="ACHUT9_001698"
     CDS             14848..15186
                     /gene="glnB"
                     /locus_tag="ACHUT9_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFR1348198.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHUT9_001699"
     CDS             complement(15231..16421)
                     /gene="hmpA"
                     /locus_tag="ACHUT9_001699"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFR1348199.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16749..18002
                     /gene="glyA"
                     /locus_tag="ACHUT9_001700"
     CDS             16749..18002
                     /gene="glyA"
                     /locus_tag="ACHUT9_001700"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFR1348200.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(18200..19393)
                     /locus_tag="ACHUT9_001701"
                     /pseudo
     CDS             complement(18200..19393)
                     /locus_tag="ACHUT9_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19511..22788
                     /locus_tag="ACHUT9_001702"
                     /pseudo
     CDS             19511..22788
                     /locus_tag="ACHUT9_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22885..23868
                     /locus_tag="ACHUT9_001703"
     CDS             22885..23868
                     /locus_tag="ACHUT9_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFR1348201.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23891..25402
                     /locus_tag="ACHUT9_001704"
     CDS             23891..25402
                     /locus_tag="ACHUT9_001704"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFR1348202.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25427..26425
                     /locus_tag="ACHUT9_001705"
     CDS             25427..26425
                     /locus_tag="ACHUT9_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1348203.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26458..27552
                     /locus_tag="ACHUT9_001706"
     CDS             26458..27552
                     /locus_tag="ACHUT9_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFR1348204.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHCATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27564..28436
                     /locus_tag="ACHUT9_001707"
     CDS             27564..28436
                     /locus_tag="ACHUT9_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1348205.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28484..>28894)
                     /locus_tag="ACHUT9_001708"
     CDS             complement(28484..>28894)
                     /locus_tag="ACHUT9_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1348206.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6866 a         7143 c         7865 g         7020 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gtgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggagaatg cccgggaatt ctcctcggtg
      241 gaaaaagaag tccattgtgt agaaacctcc cgacgatctg cttcaggaaa ccaggctgcc
      301 acagtttcgg gaagaggggg gatctgaaag ttctgccccc cttcagtgat gactctgata
      361 tggcgcgaat gtaaattttg cggcaacatc tggcgtaaaa acagtctgtt ttggtctgac
      421 agggaaatac gaaactgagg cccgttaaaa tttggaaccg aagtttgctg tattaagaat
      481 agcagagttt gatctgataa ggggttattt gcaatctcaa tagttatatg aggatctaaa
      541 cgaaaaagat ttgtcgggat tgtagttatt tggttgtttt caaaataaaa aaatcttctc
      601 atgataatat tttcttggat gtttggtaag ttgcttaatc tattgctata tgcaaaaaga
      661 agttgcagtc tcctaggtaa agggggcagt tcagtaagat ggttgtgcgc aaccttaagt
      721 atcttaagat tagaaggcag agatggaaga gtttgcagtt cgttgttcct aacctcaagt
      781 ttttttaaat tcaagggtaa tgaaggcagt gtggataacc tattatactc tgcgcttagt
      841 gatttgatgg tgtctggtaa taacggcaga ttctctaatt gattttcgga cacatccaaa
      901 gttattagat aatgtggtaa tgcagatata tgtgttaaga aattatctct tgcaataagt
      961 tctttaatat tttcaggtaa atcagtaagg atggttaatt tattacagct cacatcaagc
     1021 tttttaatgc aagcagggag tacaggtaac gaggaaagcg ccaaggctcg caggtccagt
     1081 ttatgcccct tagaggtaag acatgcacga agtcttctta ccgcctcagt acgctgttct
     1141 tccggtaacc cctgtttctc ccaatcatcc cattcggaca aataatggtt gggggatgta
     1201 attgtgctga tatgtggaaa taaacaggat gaaatatttc tgagcatatt aatttctcgc
     1261 atatggatat ggaattagat tgtgaataaa ttgtatttaa tgtatctctc ctaatgagga
     1321 gggtgattca gaatattcgc cataagggaa tgagtgttat atcatgcttt ctaccctggt
     1381 aagtatattt gcttttttcg tatatttggt tcatcttttt ttgatgtttt acatattcac
     1441 aatgcagagg atttatacca gaagacatta gtcttgagta agatactaca acatctccaa
     1501 ctattttagc cagcctgact acttgacttg gtaatatttg ttgctaataa tcatgtataa
     1561 aataagctta tatacatagt aacttcagca aaggatttag taatgatcca aatcatatct
     1621 caatgtactg tttttttctt taaaaaaaca gtacattcta ctttataatg actgtgtatt
     1681 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
     1741 gggggattta ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
     1801 ctacaacgta atgcgttgaa ctgtgcagga tgcgagctga tttttgaaga caagataagt
     1861 ggcacaaagt ccgaaaggcc ggggctgaaa aaactgctca ggacattatc ggcaggcgac
     1921 acactggttg tctggaagct ggatcggctg gggcgcagta tgcggcatct tgtcgtgctg
     1981 gtggaggagt tgcgcgaacg aggcatcaac tttcgtagtc tgactgattc aattgatacc
     2041 agcacaccaa tgggacgctt tttcttccat gtgatgggtg ccctggctga aatggaacgt
     2101 gaactgattg ttgaacgaac aaaagcagga ctggaagctg ctcgcgcaca gggacgaatt
     2161 ggcggtcgcc gtcccaaact ttcaccagaa taatgggcgc aggccgggcg actaattgca
     2221 tcaggagttt ctcgccagaa ggtggcgatc atctatgatg ttggcgaatc gacttttttg
     2281 tataagaggt ttccagccag ggttatataa aaccgcagac acggcgtata caagaacgtg
     2341 ctacggttga ctgaaaagtt aatgtggtat gtcacaggcc ttttgcgccg taaagccccg
     2401 tcctttaggg cgcggaggac gttaaagaaa gtggtgttct ttgtgttgct gaacttatgc
     2461 aaaatttttt tgcaccataa atccaacaac acattcaata acacattgct gtatcactat
     2521 gaaaataaat gtgtttttat ttgatttcac acatatcaca attgatgcat tttttagtga
     2581 ttaacaacgc cataaggaaa ctctcaaaac aacacaaacc gggcgggcat tgtacgcgca
     2641 ttctttactc agaccagttt tttcaccagc gcttcactat gacgaatacg ttctggtgcc
     2701 agttccagat cctgctgaat cagcgcaatg aacagcaaat ccgttaacat tccctgagcg
     2761 tgacaagcag agattgaagc actgtttgtc gcttgttctt cggcaatggt atacaggcaa
     2821 tgagaagcac gctgttgcag ggcattcgga gtaaagccgg taatcgccag cacttttccg
     2881 cccactcgca gcatctcatc tgccgccagg tttaactcgc gtcgtacacc ggtgtaggaa
     2941 atggctaata acagatcgtc aggggacgac gcctgtactg ttgcgagtag cgcatgcata
     3001 tcgcgcactg cggcagcatt gaagccaatc ttcatcagct tccaggcaaa gttttgcgcc
     3061 accagacccg aagcgccaat accggtcaga attatccgcc gcgcagagcg caacattgtt
     3121 acgcattcat gcagtttctc ttcactatta acgtttagcg ttgcgtacat agcggcagta
     3181 ttttctttaa tcagtttttc gccgaccagc cgtaacggat catcaccgcg gatttggtta
     3241 tgaatgggca cggagggcga ttccggctgg cttgccagcg cttcactcaa cgccaactta
     3301 agcgccggaa aacctttata gccgagtttt tgcgcgaact tcacgacgct ggactgactg
     3361 actccggctt cgttggccag ttgctgagag cttaaatggc gcgccgtatc aggttgtagc
     3421 agcagataat ccgccagttt tttatcactc tgggcaagcc cctggtaacg ctgacggata
     3481 cgaagtaagc cgttcatatc tttcctcgcg atgaaatagt ggcgacaaca ggcaatatga
     3541 gacacacgcg agtgaatttt atattccatt gccgctaatt gccatgaatt aaaaattcca
     3601 gttagccatg atgcggtcga ttgttgccgg aaaagtgatg atctatctgt taggctatgt
     3661 agtagcgaaa attaatcagg atgtttcagt ccagaggagt atggtttggc aactcacgag
     3721 cgtcgtgtgg tgttttttga cttagatgga acattgcatc agcaggatat gttcggcagt
     3781 tttctgcgct atttactgcg tcgccaaccg ctgaatgcgt tacttgtcct gccgttgtta
     3841 ccgattatag ccattgcgtt attgataaaa ggtcgtgcgg cacgctggcc gatgagtctg
     3901 cttctgtggg ggtgcacttt tggtcacagc gaagcacgtt tacagacgtt gcaggccgat
     3961 ttcgtgcgct ggtttcgcga caatgttacc gcctttccgc tggttcagga gcgattaacc
     4021 acctacctgt taagttccga tgctgatatc tggttgatta ccggctctcc gcagccgctg
     4081 gttgaagcgg tttatttcga tacgccctgg ctgccgcggg ttaatcttat cgccagccaa
     4141 attcagcgtg gctatggtgg ttgggtattg acgatgcgtt gtctgggaca tgaaaaggtc
     4201 gcacaactgg agcgcaaaat cggcactccg ctgcggctgt acagtggcta tagcgacagt
     4261 aatcaggaca atccgctgct ttatttctgt cagcatcgtt ggcgagtaac cccgcgcggt
     4321 gaactccagc aactggaata gagtaaagca tagcgtccgt gtataatgcg ccgcgctttt
     4381 ataaccggag ttttcttttt gtctgaagtc gaatttagcc acgaatactg gatgcgtcac
     4441 gcgctgacgc tggcgaaacg tgcctgggat gagcgggaag tgccggtcgg cgcggtatta
     4501 gtgcataaca atcgggtaat cggcgaaggc tggaaccgcc cgattggtcg ccatgatccc
     4561 accgcacatg cagaaatcat ggccctgcgg cagggtggtc tggtgatgca aaattatcgt
     4621 ctgatcgacg ccacgttgta tgtcacgctt gaaccatgtg taatgtgtgc cggagcgatg
     4681 atccacagtc gcattggtcg cgtggtcttt ggtgcgcgtg acgcgaaaac tggcgctgcg
     4741 ggatctttaa tggatgtgct gcatcatccg ggtatgaatc accgagtgga aattacggaa
     4801 ggaatactgg cggatgagtg cgcggcgttg ctcagtgact tctttcgcat gcgccgccag
     4861 gaaattaaag cgcagaaaaa agcgcaaccc tcgacggatt aattttgttt ctcttcactc
     4921 cctttcctgg aaaacagcag agagggagaa tgggtcaaat agtttgatga cgactgggaa
     4981 agaaacgaga gaaaaggaaa tttctctttg cccaactccg taggcgatac cgccggataa
     5041 tcctgcgcca gttgcatcgc cgcttctgta gcctgcttct ctttctcttg cagataaccc
     5101 accaggctaa tctgatactt acgaatattt tcgacataag cgtaggcttc atgtccacga
     5161 gcgtagccgt aagtcagctt gctgtaatag ggtttctggc taagtaaagg cagacgctgt
     5221 tttacgtcag cccaactgtc aggattccct ttggtttttg ccgtcagggc gcgggcatcc
     5281 agcatatgcg catagcccat attgtacgca gcgagggcaa accagatccg ctcgttctcc
     5341 ggcacacttt ccggcacttt actcatcata tcctgcaaat aacgcacgcc accgctgatg
     5401 ctctgttcgg catcggtacg atccgtaatg ccgaggcttt gcgcggtatt tttggttaac
     5461 atcatcatgc cgcgcacacc cgtcggtgaa gtggcctgtg catcccagtg cgattcctga
     5521 taagcaatag cggccagcaa acgccagtca atttcttcgg cgtatttctc aaacaggggc
     5581 tttaactgcg gcagtaccgc atcgacggcg cgtaaaaatg tgcgcgtatc gacgtaatca
     5641 aaatcatcgc catgccccag gtatttctct tcaatgcgtg ccagcgtaca gtcttcattc
     5701 atttcgttga agaagtcgag cagggcggcg gaaagggtat tatcgccatc taacgggcta
     5761 aaccaagtca ccggttgttc atcggtgata tcgagcgcta cggcgagctc cgggtgaacg
     5821 cgctgaaaca ggctgatggc gacagaatca gcaatggtgt aatcgagttt tccttcgatg
     5881 acatcttcca ttaattccgc agagcctttt ttgtcgtcta ccttccagct taattccggg
     5941 aattttgtgt ctttcagggt ttggagatcg ttaaccacca cgtgacccgg tgcaacggtg
     6001 agttgctccg ccgtcaggtt gcccagtgtg cgcgggcgat actgactcac tttataaacc
     6061 agttgttgtg acacggaata ataggtaggg ccaggctgat aattttttac ccgctcactg
     6121 ttatagacca gtcctgccgc cagcaggtcg gcgttaccat tatcgaggtc gtcaaacagc
     6181 tggctgatat tctgccgcac ggtcactttc agttttacac cgaggtaatc ggcaaactgt
     6241 ttcgccagtt cgtaatccag gccaaaaggt ttcccgttga tttcgttata agtcagggga
     6301 gtatgaatgg tgctcacacg caactctccc cgcgcttgaa tggcggcgat acggttgtcg
     6361 gctttaccaa accagggaat ggatggccag agagcgaccg cgagcagcag tgccagaatg
     6421 ccgatgaaca gataattaat ctttaatttt ttcaattagt taattctctg tgtcgtgcgc
     6481 gtcccagctt gaaaaaacgt aataatagtg aaaggtttac tcataaatga gcggcatttt
     6541 gcgtaaacct gcgccagatg gcaacttatt acagccattg gcggcacgcg ttgctaattc
     6601 acgatggtga ttttatttcc acgcaaacgg tttcgtcagc gcatcagatt ctttataatg
     6661 acgcccgttt cccccccttg ggtacaccga aagcttagaa gacgagagac ttatgatgga
     6721 aattctgcgt ggttcgcctg cactgtcggc attccgaatc aacaaactgc tggcacgttt
     6781 tcaggctgcc aggctcccgg ttcacaatat ttacgccgag tatgtccatt ttgctgacct
     6841 caatgcgccg ttaaacgatg atgagcacgc acaacttgaa cgtctgctga aatatggccc
     6901 ggcactcgcc agccacgccc cgcaaggcaa actcctgctg gtgaccccgc gtcctggcac
     6961 catctctccc tggtcttcga aagcgaccga tattgcccat aactgcgggc tacaacaggt
     7021 aaaccgcctt gagcgcggcg ttgcttacta tatagaagcc ggtacgctga ccaatgaaca
     7081 atggcagcag gttaccgctg aactgcacga ccgcatgatg gaaacggtct tttttgcttt
     7141 agatgatgca gagcagttgt ttgcccacca tcaaccgact ccggttacca gcgttgattt
     7201 gctggggcag ggccgtcagg cgctgatcga cgctaacctg cgtcttggcc tggctctggc
     7261 ggaagatgaa attgactatc tgcaggatgc tttcacaaag cttggtcgta acccgaacga
     7321 catcgagctg tatatgttcg ctcaggcgaa ctctgagcac tgccgccaca aaatttttaa
     7381 tgccgactgg attatcgacg gtgaacagca gccgaaatcg ctgttcaaga tgatcaaaaa
     7441 tactttcgaa accacgccag atcacgttct ctctgcttat aaagataacg ccgccgtaat
     7501 ggaaggttct gaagtgggcc gctactttgc tgaccacgaa acgggccgct acgatttcca
     7561 tcaggaaccg gcgcatattc tgatgaaagt cgaaactcac aaccacccga cggcgatttc
     7621 tccgtggccg ggcgcggcga cgggttccgg cggcgaaatt cgtgatgaag gcgccaccgg
     7681 acgcggtgcg aagccgaagg ctggtctggt tggtttctcc gtttccaacc tgcgaattcc
     7741 tggcttcgaa cagccgtggg aagaagattt cggtaagcct gagcgcattg tcaccgcgct
     7801 ggacatcatg accgaaggcc cgctgggcgg cgcggcgttt aacaacgaat ttggtcgtcc
     7861 ggcactgaat ggctacttcc gtacttatga agaaaaagtg aacagccaca acggcgaaga
     7921 gctgcgcggt tatcacaaac cgatcatgct ggcgggcggg atcggcaaca ttcgcgccga
     7981 tcacgtacaa aaaggcgaga tcaacgtcgg tgcgaagctg gtcgttctcg gcggcccggc
     8041 aatgaacatc ggtcttggcg gcggagcggc gtcttccatg gcgtcaggtc agtctgatgc
     8101 cgacctcgac tttgcttccg tacagcgcga caacccggag atggagcgtc gctgccagga
     8161 agtgatcgac cgttgctggc agcttggtga tgccaaccca atcctgttta tccacgacgt
     8221 cggcgctggc ggtctttcta acgcgatgcc ggaactggtg agcgacggcg ggcgcggcgg
     8281 taaatttgaa ctgcgcgata ttctaagcga cgaaccgggc atgagcccgc tggaaatctg
     8341 gtgtaacgaa tcccaggaac gctacgtgct ggcggttgct gccgatcaat taccgctgtt
     8401 tgacgaactg tgtaagcgtg agcgcgcacc ctacgcggtg attggtgaag cgaccgaaga
     8461 actgcatctt tctctgcacg atcgtcattt tgataatcag ccgatcgatc tgccgctgga
     8521 cgtcctgctt ggtaaaacgc cgaagatgac ccgcgatgta caaacgctga aagcgaaggg
     8581 cgatgcgctg gtccgtgaag ggatcaccat tgcagacgcg gtgaaacgcg tactgcatct
     8641 gccgaccgtg gcagagaaaa ccttcctggt gaccatcggc gaccgcagcg taaccggcat
     8701 ggtagcgcgc gatcagatgg tggggccgtg gcaggtgccg gtcgctaact gcgcggtcac
     8761 taccgccagc ctcgacagct actacggtga agcgatggcg attggcgagc gtgcgccggt
     8821 tgcactgctg gatttcgccg cctctgcccg tctggcggtc ggtgaagcgt taaccaatat
     8881 tgccgcaaca cagattggcg atatcaaacg cattaaactt tccgccaact ggatggcggc
     8941 ggcaggccac cctggtgaag atgcgggcct gtatgaagcc gttaaagccg tgggcgaaga
     9001 gctttgtccg gcgctgggcc tgacgatccc ggtgggtaaa gactctatgt cgatgaaaac
     9061 ccgctggcag gaaggtaacg aagagcgcga aatgacgtcg ccgctgtcgc tggtgatttc
     9121 tgcatttgcc cgcgtggaag atgtgcgtca caccatcacg ccacagcttt ctaccgaaga
     9181 taacacactg ctgctgattg atttgggcaa aggcaataac gcgctgggcg caacggcgct
     9241 ggcgcaggtt tatcgtcagc ttggcgacaa accggcagat gtacgcgacg tcgcgcaact
     9301 gaaaggcttc tatgacgcga ttcaggcgct ggttgcacag cgtaagctgc tcgcatacca
     9361 cgaccgctct gatggcggcc tgctggtaac gctggcggaa atggcctttg ctggtcattg
     9421 cggcattaac gcggatattg cctctctggg cgacgatcgc ctggcggcgt tgtttaacga
     9481 agaactgggt gcggtgattc aggttcgtgc cgctgaccgt gaagcggtcg agtccgtact
     9541 ggcacagcat gggcttgctg attgtgtcca ttatgtaggg caggcggttt ccggtgaccg
     9601 ttttgtgatt accgccaacg ggcagactgt attcagcgaa agccgcacca cgttgcgtgt
     9661 ctggtgggca gaaactacct ggcagatgca gcgcctgcgt gacaacccgg agtgtgctga
     9721 ccaggagcac caggcgaaat caaacgacgc cgatccgggc ctgaatgtga aactgtcgtt
     9781 cgatatcaac gaagatgtgg cagcaccgta tattgccact ggcgcacgtc cgaaagttgc
     9841 tgtgctgcgt gagcagggcg tgaactcgca tgttgaaatg gcggcagctt tccaccgtgc
     9901 aggctttgat gctatcgacg tgcatatgag tgacctgctg accggacgca cgggcctgga
     9961 agatttccac gccctggtcg cgtgcggtgg tttctcctac ggtgatgtgc tgggtgccgg
    10021 tgaaggttgg gcgaagtcaa tcctgttcaa tgaccgtgta cgcgatgagt ttgcaacctt
    10081 cttccaccgt ccgcaaacgc tggcgctggg ggtatgtaac ggttgccaga tgatgtctaa
    10141 cctgcgtgaa ctgatcccgg ggagcgagtt gtggccacgt tttgtgcgta acacctccga
    10201 tcgctttgaa gcgcgtttca gcctggttga agtaacccaa agcccgtctc tgctgttgca
    10261 ggggatggtg ggctcgcaaa tgccgattgc tgtctctcat ggtgaagggc gcgtggaagt
    10321 gcgtgatgcg gcgcatctgg cggcactgga aagcaaaggg ctggtggcac tgcgctatgt
    10381 cgataacttc ggcaaagtca ctgaaaccta cccggctaac ccgaacggtt caccgaacgg
    10441 tattacggcg gttacgactg aaagtggtcg agtcaccatt atgatgccgc acccggaacg
    10501 tgttttccgt actgtcagca actcctggca tccggaaaac tggggcgagg atggcccatg
    10561 gatgcgcatt ttccgcaatg cgcgtaagca gttggggtaa gtcatcagtt cattggtttt
    10621 gcagcccgga tgcggcatta agcaccaacc actatcataa gctcccaact ccgggagctt
    10681 ttttgtgtct gtaaatcacg acaatgggtg gtttaccgtg tcgctttctg gcgacactta
    10741 aatgattgat tttattgtaa tctaataagt ttatcttaag ttgtctcttt ttagcgacac
    10801 agtggctgat ttacattcaa atagcgaaga agccataaat ccctcaaagc aacgtataat
    10861 cagtacgtta ccaaactatt ttctttattg gcacagttac tgcataatag taagcagtgg
    10921 ctcattcacc gacttatgtc agccccttcg ggacgtgcta cataaaattc gaatgacgca
    10981 caacaaggtg cctgccgtcc aacttctgat atcagcgtag ctatatcaac catcgggcga
    11041 aacgtcgagt taggcaccgc cttattccat aacaaagccg ggtaattccc ggctttgttg
    11101 tatctgaact tcccctcggt tagcatcagg ctattcgcgt ctgacgagag taacaccttg
    11161 aaacgctggc ccgtttttcc ccgctcatta cgacaactgg taatgctggc atttttgcta
    11221 attctactac ccctgttggt gctggcatgg caagcctggc aaagcctgaa tgcgcttagc
    11281 gatcaggcgg cgctggttaa ccgcactacg cttattgatg cccggcgtag tgaagcgatg
    11341 accaacgcgg cgctggagat ggagcgtagc taccgtcagt attgcgtgct ggacgaccct
    11401 acgcttgcga aggtttatca aagccagcgc aagcgttaca gcgaaatgct cgatgcccac
    11461 gcaggcgtac tgccagacga taaactttac caggcattgc gtcaggattt gaacaatctg
    11521 gcccaacttc agtgtaacaa cagtggtccc gatgctgccg ccgccgcgcg tcttgaagcc
    11581 tttgccagtg ccaataccga aatggtgcag gccacgcgca cagtggtgtt ctctcgtggg
    11641 cagcaacttc agcgtgaaat cgccgaacgt gggcaatatt ttggttggca atcgctggtg
    11701 ctatttctgg tgagtctggt gatggtgcta cttttcacgc ggatgattat cgggccggtg
    11761 aaaaatatcg agcgcatgat caaccgtctg ggggaagggc gttctctggg caatagcgtt
    11821 tcgttcagtg gaccgagcga gttacgctcg gttgggcaac gtattctttg gttaagtgag
    11881 cgcctgtcat ggctggaatc ccaacgccat caatttttaa gacatttatc tcatgaatta
    11941 aaaacgccac tggcgagtat gcgcgagggc actgaattac tggctgacca ggttgtcggg
    12001 ccgcttacgc cagagcaaaa agaggtggtg agcattcttg atagcagcag ccgcaatttg
    12061 caaaaactga tcgaacaact gcttgattac aaccgtaaac aggcggacag tgcggtggaa
    12121 ctggagaatg ttgaattagc accgctggtg gagacagtgg tttctgctca tagcctgccc
    12181 gcacgggcta aaatgatgca taccgacgtc gatctcaaag caacagcttg cctggcggag
    12241 ccaatgctgc tgatgagcgt actggataat ctttactcca atgcggtgca ctacggggct
    12301 gaatccggta acatttgcct tcgcagcagt tcacatggtg cgcgggttta tattgatgtc
    12361 atcaatacag gcacgcccat tccgcaagag gaacgcgcca tgatcttcga accctttttt
    12421 cagggaagcc accagcgaaa aggggcggtg aagggcagcg gtctgggatt aagcattgcc
    12481 agggattgta ttcgccgtat gcaaggggaa ctgtatctgg tcgacgagag cgggcaagac
    12541 gtttgtttcc gcattgaatt accgtcgtcg aaaaacacga aataatgcta acagcctatc
    12601 ctatcaggct attttatttg tcagttcggc cccgggcagt gctcgaaatc ttcacgtacg
    12661 atatgtacgc tccggtttct ccgcgctgtc catgtccaga ctgtctgcaa caattacgcc
    12721 cactgggata ggctttaagt ctggtgaata tgcgacacat ttttcaacga ttattgcccc
    12781 gacggctgtg gctggcaggg ctgccatgtc tggcattgct gggttgcgtg cagaatcaca
    12841 ataagccagc cattgatacg ccagcagaag aaaaaattcc ggtttatcaa ctggctgact
    12901 atctttctac tgaatgtagc gacatctggg cgctgcaagg taaatcaacc gaaaccaatc
    12961 cgctttactg gctgcgggcg atggattgtg ctgatcgttt aatgcctgcg cagtcgcgtc
    13021 agcaagctcg ccaatatgac gacggcagct ggcaaaatac ctttaaacaa gggattttgc
    13081 tcgccgacgc caaaattacg ccgtatgagc gccgccagct ggtcgcgcgt attgaagcgt
    13141 taagtaccga gatcccggca caggttcgcc cactgtatca actctggcgc gacggtcagg
    13201 cgctgcaact gcaactggcg gaagagcgcc aacgttacag taaactccag cagtccagcg
    13261 acagcgagct ggatacattg cgccagcaac atcacgttct acagcagcaa ctggaactca
    13321 ccacccgcaa gctggaaaac ctgaccgata ttgaacgcca gctctcgacc cgcaaaccga
    13381 ctggaaattt ctcgccggat acgccgcacg agagtgaaaa gcccgcgcca tccacccatg
    13441 aggtcactcc tgatgagcca taaacctgcg catttattat tggtcgatga cgatccggga
    13501 ttgctgaaac tgcttggcct gcgcctgacc agcgaaggct acagtgtggt cacggcggaa
    13561 agtggcgctg aaggattacg ggtactgaat cgcgaaaaag tagatttagt tatcagcgac
    13621 ctgcggatgg atgaaatgga cggtatgcag ctatttgctg aaatccagaa agtgcagccg
    13681 ggaatgccag taattattct tactgcgcat ggttctatcc ccgatgccgt tgctgcaaca
    13741 cagcagggcg tttttagttt cctcaccaag cctgtcgata aagatgcgct atatcaggca
    13801 attgacgatg cgctggagca atccgcgcca gccaccgatg aacgctggcg cgaggcaatt
    13861 gtcacccgca gcccgctgat gctgcgtttg ctggaacagg cgcggctggt ggcgcaatca
    13921 gacgtcagcg ttttgattaa cggtcagagc ggcaccggga aagagatttt cgcccaggct
    13981 atccacaacg ccagcccgcg caacagcaaa ccatttattg ctattaactg tggcgcattg
    14041 cccgagcaat tgctggagtc ggagctgttt ggtcatgcgc gtggcgcgtt tactggcgct
    14101 gtcagcaatc gcgaaggttt attccaggcg gcggaaggcg gaacgctatt tctcgatgaa
    14161 attggcgata tgcctgcgcc gttacaggtc aaactgctgc gcgtgttgca ggagcgtaaa
    14221 gtgcgcccgc tgggcagtaa ccgcgatatt gatatcaatg tgcggattat ttctgccact
    14281 caccgtgatc tgccaaaagc gatggcgcgc ggggaattcc gcgaagatct ctattaccgc
    14341 ctcaacgttg tcagcctgaa aattccggca ctggcggagc gcacagaaga cattccgcta
    14401 ctggcaaatc acctgttgcg ccaggcggca gagcgacata aaccgtttgt ccgcgcgttc
    14461 tctaccgatg cgatgaaacg cctgatgacc gcgagctggc cgggtaatgt gcgccagttg
    14521 gtcaacgtga ttgaacagtg cgtggcgctg acctcatctc cggtgattag tgatgcgctg
    14581 gtggagcagg cgctggaggg tgaaaatacg gcgctgccaa cctttgttga ggcacgtaat
    14641 cagtttgaac tcaactattt gcgtaagctg ctgcaaatca ccaaaggcaa cgtcacccac
    14701 gcggcgagaa tggcggggcg caaccggaca gaattttata aactgctttc ccgacacgag
    14761 ctggatgcaa acgatttcaa ggaatgaatt ggcgttatgt gttacgttta gcagatcaaa
    14821 agacaggcga ccttttcaag gaatagcatg aaaaagattg atgcgattat aaaacccttc
    14881 aagctggacg atgtccgcga agcactggcc gaagtcggta ttaccggcat gacggtgacc
    14941 gaagtgaaag gctttggtcg ccagaaaggc cataccgagc tgtaccgcgg cgcggagtat
    15001 atggtggatt ttctgccgaa agtgaaaatt gagattgtcg taccggacga cattgtcgat
    15061 acctgtgtcg ataccattat tcgcacggcg caaaccggca aaatcggtga cggtaaaatc
    15121 ttcgtctttg acgtggcacg ggtcattcgc atccgtaccg gtgaagagga cgacgcggca
    15181 atttaaaccg cgtcaagggt tgccggatgt ttccatccgg caacatcaaa ttacagcacc
    15241 ttatgcgggc caaagcattc gtaatgaatg ttttcctgct tcacgcccag atccactaac
    15301 tgtttcgcgg taaactgcat gaagccaacc gggccgcaga gatagaactg cattgtcgga
    15361 tcgctgaacg caccttccag tttgctcaaa tccatcagac cttcgctatc aaactgacct
    15421 ttagcgcgat cggcttcgct cggctgacga tgccaggtgt gcgcggtaaa gcgcggcagt
    15481 gactgcccca gttccttaac ttcatcggca aaggcgtgaa catcgccatt ttctgccgca
    15541 tggaaccagt tcacttgtgc tgtgtggcct gcttttgcca gcgtgtcgag cattgccagc
    15601 attggcgttt gaccaacacc ggcagagatt aacgtcactg gtgtgtcatc tgcgacagcc
    15661 ataaagaaat cacctgccgg agcgaccagt ttcacgacat cgccaacatt ggcgtgattg
    15721 tgcaaccagt tggatacctg cccaccctct tcgcgtttca ccgcaatacg atagcctttg
    15781 ccatccggtt tgcgagtcaa agagtactga cgaatttcct gatgcgggaa accttccggc
    15841 ttcagccaga cgccgagata ttgccccgga cggtattctg ccactgcgcc accgtcgacc
    15901 ggctccagtt cgaagctggt gataagcgcg ctgcgcggtg ttttagccac aatgcggaaa
    15961 tcgcgagtac cttcccaacc accggctttg ctggcgtttt cgttatagat ttccgcctcg
    16021 cgattgataa atacattagc cagtacacca taggctttac cccacgcgtc cagcacttcc
    16081 tgccccgggc tgaacatttc gtccagcgtt gccaacaggt gttcaccgac gatgttgtac
    16141 tgttccggtt tgatctggaa gctggtgtgc ttctgcgcga ttttttctac cgctggcagc
    16201 agcgcaggca ggttttcaat attactggcg taggcggcaa tagcgttaaa cagggcttca
    16261 cgttgatcgc cattacgctg gttactcatg ttaaaaattt ctttgagttc tgggttatga
    16321 gtaaacatac ggtcgtagaa atgggcggtt aactttggcc ccgtttccac cagtaaaggg
    16381 atggtggctt ttactgtagc gatggtttga gcgtcaagca tatggtcttc ctttttttgc
    16441 atcttaattg atgtatctca aatgcatctt ataaaaaata gccctgcaat gtaaatggtt
    16501 ctttggtgtt tttcagaaag aatgtgatga agtgaaaaat ttgcatcaca aacctgaaaa
    16561 gaaatccgtt tccggttgca agctctttat tctccaaagc cttgcgtagc ctgaaggtaa
    16621 tcgtttgcgt aaattccttt gtcaagacct gttatcgcac aatgattcgg ttatactgtt
    16681 cgccgttgtc caacaggacc gcctataaag gccaaaaatt ttattgttag ctgagtcagg
    16741 agatgcggat gttaaagcgt gaaatgaaca ttgccgatta tgatgccgaa ctgtggcagg
    16801 ctatggagca ggaaaaagta cgtcaggaag agcacatcga actgatcgcc tccgaaaact
    16861 acaccagccc gcgcgtaatg caggcgcagg gttctcagct gaccaacaaa tatgctgaag
    16921 gttatccggg caaacgctac tacggcggtt gcgagtatgt tgatatcgtt gaacaactgg
    16981 cgatcgatcg tgcgaaagaa ctgttcggcg ctgactacgc taacgtccag ccgcactccg
    17041 gctcccaggc taactttgcg gtctacaccg cgctgctgga accaggtgat accgttctgg
    17101 gtatgaacct ggcgcatggc ggtcacctga ctcacggttc tccggttaac ttctccggta
    17161 aactgtacaa catcgttcct tacggtatcg atgctaccgg tcatatcgac tacgccgatc
    17221 tggaaaaaca agccaaagaa cacaagccga aaatgattat cggtggtttc tctgcatatt
    17281 ccggcgtggt tgactgggcg aaaatgcgtg aaatcgctga cagcatcggt gcttacctgt
    17341 tcgttgatat ggcgcacgtt gcgggcctgg ttgctgctgg cgtctacccg aacccggttc
    17401 ctcatgctca cgttgttact accaccactc acaaaaccct ggcgggtccg cgcggcggcc
    17461 tgatcctggc gaaaggtggt agcgaagagc tgtacaaaaa actgaactct gccgttttcc
    17521 ctggtggtca gggcggtccg ttgatgcacg taatcgccgg taaagcggtt gctctgaaag
    17581 aagcgatgga gcctgagttc aaaacttacc agcagcaggt cgcgaaaaac gctaaagcga
    17641 tggtagaagt gttccttgag cgcggctaca aagtggtttc cggcggcact gataaccacc
    17701 tgttcctggt tgatctggtt gataaaaacc tgaccggtaa agaagcagac gccgctctgg
    17761 gccgtgctaa catcaccgtc aacaaaaaca gcgtaccgaa cgatccgaag agcccgtttg
    17821 tgacctccgg tattcgcgtg ggtactccgg cgattacgcg tcgcggcttc aaagaagcag
    17881 aagcgaaaga actggctggc tggatgtgtg acgtgctgga cagcatcaat gatgaagccg
    17941 ttatcgagcg catcaaaggt aaagttctcg acatctgcgc acgtttcccg gtttacgcat
    18001 aagcgaaacg gtgatttgct gtcaatgtgc tcgttgttca tgccggatgc ggcgtgaacg
    18061 ccttatccgg cctacaaaac tttgcaaatt caatatattg caatatccgt gtaggcctga
    18121 taagcgtagc gcatcaggca atcttaggta tgtgatcatc aaggcttcct tcgggaagcc
    18181 tttctacgtt attgcgccat caaatctgtc gtaactgcgc ctcaacatac aaatagccaa
    18241 tccccaacac ctgttgtgcg cggcttaatt gcccaaagcc aatttgcgtt gctttcacgc
    18301 tcggcccttc gtcgcggtcg aacgggttaa atcccgtctg gcgaataatg gtattaagcc
    18361 agtgctcacc aaaggcacaa ctgcgaccgt acaaccaaat ctgattaata ttgaggatgt
    18421 tgaggaagtt atacagactc aaaccaatgg cattggcact gcgatcaacc caactggtga
    18481 tccacggttc tccactctac caggcagcga ttaactgcgc tgtagtcagt ttttccggat
    18541 caagctgagt attaaccggt tgtgatttta gccatacccg cgcctgtttt tttaatgcac
    18601 ttaacgaggc gacggtttcc aggcagccgt aacgtccaca gtcgcagacg acgccatccg
    18661 gattaacgat ggtgtgacca atctgtccgc taccatacaa actgccgcga taaatttgct
    18721 cgttaatcac gaacgacgag ccaatgccgt aatcaacgtt gatcacgcag aaatcccgtt
    18781 tctgcgaatt attttgccat ttctccgcca gcgccagcat cacgcagtca ttatcgacca
    18841 tcacccgaat gccgagcttc tcttccagca gatacttcac ctcaaccggc gttgtccacg
    18901 gcgcttgcgg catggtttgc gacacgccgg tcaccggatc aacctgaccg tggattgcca
    18961 gcgccagatt gatggtgcgg tccggccaca atttacggtg gcgatgccag catttttcga
    19021 tttcggacag cagcgcctgc ggcgtcggtg catcaatctg taaatactca aattcacctt
    19081 tcggacttaa acaagcatta gcaacctgac actcaatact ggtgggcgtc acgttcaggc
    19141 acagcgtcca gtcaccttcc ggcgcaatca gccatgtacc gctactatgc ccgcgcgtct
    19201 ggctttcatc gtcgatattc accacccgtt tttcgctttc cagttcctgc aaaatattac
    19261 tgactgccgg aatcgaaatt tgcgccagcc gggccagcgt tgatttattg gcgcgctttt
    19321 gccggtacag cagttccaga atcacgcatt tgttatggtg gcgaatctgt tgattgttaa
    19381 tgcaggctct catcttcact aaaccttgtt tagttaattg cgtgattatt ccgcgaaaac
    19441 attgccagat tgttcaatac actgccacaa atcttttaat tcagtatgtc ttgttaatat
    19501 tgagggcacc atgactccag taaaagtgtg gcaagagcgc gttgagatcc cgacctatga
    19561 aaccgggccg caggatatac atcccatgtt cctggaaaat cgcgtttatc agggatcgtc
    19621 cggcgcggtt tatccctacg gcgtgaccga tacgctgagc gagcagaaaa ccctgaaatc
    19681 ctggcaggcg gtgtggctgg aaaacgacta catcaaagtg atgatcctgc cggaactggg
    19741 cggtcgggtg catcgcgcgt gggataaagt gaaacagcgc gattttgttt atcacaatga
    19801 agtcattaaa cctgcgctgg tggggctgct ggggccgtgg atttccggtg ggattgagtt
    19861 taactggccg caacatcatc gcccgaccac ctttatgccc gttgatttca ccctcgaagc
    19921 ccatgaagac ggtgcacaga cggtgtgggt aggcgaaacg gagccgatgc atggtttaca
    19981 ggtgatgaca ggtttcaccc tgcgccctgg gcggcgctgg aaatcgccag ccgcgtctat
    20041 aacggcaacg ccacgccgcg tcatttcttg tggtgggcca acccggcagt gaaagggggt
    20101 gaagggcatc agagcgtttt cccaccggat ttgactgcgg tgtttgatca cggcaaacgg
    20161 gccgtctccg ctttccccat cgctaccggc acttactaca aagtggacta ctccgctgga
    20221 gtggacattt ctcgctataa aaatgtgccc gttccaacct catatatggc cgaaaaatcg
    20281 cagtacgatt ttgtcggcgc gtggtgtcac gatgaagatg gcggtttgct gcacgttgcc
    20341 aaccaccata ttgcgccagg taaaaaacag tggagttggg gacacagtga atttggccag
    20401 gcgtgggata agagtctgac cgacaataac ggcccgtata tcgaactgat gaccggtatt
    20461 tttgccgata accagcccga ttttacctgg cttgatgctt acgaagagaa gcgttttgag
    20521 cagtatttcc tgccttatca ttctctgggc atggtgcaaa atgcctcccg cgatgcggtg
    20581 ataaaactcc agcgtagtga gcaggggatt gagtgggggc tgtatgccat ctctccgttg
    20641 aacggatacc gcctggcgat ccacgaaatc ggcaaatgca acgcgttact tgatgatgcc
    20701 gtggcactga tgcctgcgac cgccatccag ggcgtgttgc acggtatcaa tcctgatagg
    20761 ctgaccattg agctttccga tgccgacggc aatattgtac tgagttatca ggaacatcag
    20821 ccgcaagcgt tgccgttacc ggacgtcgcc aaagcgccac tggcagcaca agacattacc
    20881 agtacagatg aagcctggtt tatcggtcag catctggagc aatatcatca cgccagccgt
    20941 tcaccgttcg attactacct gcgcggcgtg gcgctggacc cgctggatta tcgctgtaac
    21001 ctggcgctgg cgatgctgga atataaccgc gcagatttcc cgcaagcggt ggcgtatgcc
    21061 agtcaggcgc tgaaacgcgc acatgcgctg aacaaaaacc cgcagtgtgg acaagcaagt
    21121 ttgattcgcg ccagtgctta cgaacgtcag ggacaatatc aacaagccga agaggatttc
    21181 tggcgtgcgg tctggagcgg caacagcaaa gccggtggct attatggcct ggcacgactg
    21241 gcggcgcgta atggtaactt cgacgctggt ctggattttt gccaacaaag tcttcgcgcc
    21301 tgcccaacca atcaggaagt gctttgcctg cataatctgc tgctggtgtt aagtggtcgt
    21361 caggacaacg cgcgtttgca gcgcgcgaaa ctgctgcgtg attatccgct gaacgccatt
    21421 ctgtggtggc tgaactggtt cgatggtcgt agcgaatcag ccctcgcgca gtggcgcggt
    21481 ctgtgtcagg gacgcgacgt taacgccctg atgaccgccg ggcaactgat taactgggga
    21541 atgcccactc tggcgacaga gatgctgaac gcactggact gtcagcgcac gttatcgctt
    21601 tatctacaag ccagcttgct gccgaaagcc gaacgcggcg aactggtcgc aaaagccatt
    21661 gatgtcttcc cgcagtttgt ccgtttcccg aatacgctgg aagaagtggc ggcgctggag
    21721 agtattgaag agtgctggtt tgcccgtcat ttactggcct gcttctacta caacaagcgc
    21781 agctacaaca aagccattac tttatggcaa cgttgcgtag agatgtcgcc ggagtttgcc
    21841 gatggctggc gcgggttggc gatccatgcg tggaataagc aacacgatta tgaactggcc
    21901 gcgcgttatc ttgataatgc ttatcagctt gcgccgcagg atgcacgtct gcttttcgaa
    21961 cgggatttgc tggataagtt aagtggagcc acaccggaga aacgactggc gcgtctggaa
    22021 aataatctgg aaattgcgct gaaacgcgac gacatgaccg cagaactgct caatttgtgg
    22081 catctcacgg gtcaggcaga caaagcggcg gacattctcg ccacgcgcaa attccacccg
    22141 tgggaaggcg gggaagggaa ggtcaccagt cagtttatcc tcaaccagtt attacgcgcc
    22201 tggcagcatc ttgatgccag acagccgcag caggcctgcg aactgcttca tgccgcgctg
    22261 cattatccgg agaatttaag cgaaggccgt ttaccggggc aaactgataa cgacatctgg
    22321 ttctggcagg cgatatgcgc caacgcgcag ggcgatgaaa ctgaagcgac gcgttgttta
    22381 cgtctggcgg cgaccggcga tcgcaccatt aacatccaca gttattacaa cgatcagccg
    22441 gttgattatc tcttctggca aggaatggcg ctgcgactgc tgggcgaaca acaaatcgca
    22501 cagcaactgt ttagtgaaat gaaacagtgg gcgcaagaga tggcgaaaac cagtattgag
    22561 gcggatttct ttgctgtttc acaacctgac ctgttgtcgc tgtatggcga tttacaacag
    22621 cagcataaag aaaaatgcct gatggtggcg atgctggcgt ccgcgggact cggggaggtt
    22681 gcgcaatatg aatctgctcg cgctgaattg acggcgatta atccggcctg gccgaaagcg
    22741 gcattattca ccaccgtgat gccttttatt tttaactacg ttcactaaac cctattaact
    22801 gtaatatttg agcggcacgc gctgccgctc atcacaaaat aattagaata agccgttgtt
    22861 aataaaaact ctacaggagg cactatgcct aaaaaaatga gaacaacccg taatctatta
    22921 ttaatggcta cgctattagg tagcgcacta tttgccaggg ctgcggataa agaaatgacc
    22981 attggtgcaa tataccttga taccctggga tattacgctg gagtgcgtca gggcgttcag
    23041 gatgcggcaa aagattcttc agtacaggta cagttaattg aaactaacgc ccagggtgat
    23101 atttcgaaag aaagtacctt tgttgatacc ctcgtggcgc gtaatgtcga tgccattatt
    23161 ttatcggcag tgtctgaaaa tggcagtagc cgtaccgttc gtcgcgccag tgaagcgggt
    23221 attccggtga tttgctacaa cacctgtatt aatcaaaagg gtgtcgataa atatgtctcg
    23281 gcgtatctgg tcggcgatcc actggaattt ggtaaaaaac tgggtaacgc tgccgccgat
    23341 tattttattg ccaataaaat tgaccagccg aaaattgccg tcatcaattg cgaagccttt
    23401 gaagtttgtg tgcagcgacg taaaggattt gaagaagtat taaaatcccg cgttcccggc
    23461 gcgcaaattg tcgctaatca ggaagggact gttttaaata aagcgatttc cgttggtgaa
    23521 aaactgatta tctccacgcc ggatctcaac gccattatgg gggagtcggg cggtgcgaca
    23581 ctcggcgcgg taaaagcggt acgtaatcaa aatcaggccg gaaaaattgc tgttttcggt
    23641 tcggatatga caaccgaaat tgctcaggag ctggaaaaca atcaggtgct gaaagcggta
    23701 gtggatattt ccggtaagaa aatgggcaat gctgttttcg cgcaaacatt gaaggttatc
    23761 aataaacaag ccgacggtga aaaagtgatt caggtgccaa tcgatctcta taccaaaacg
    23821 gaagatggta aacagtggct ggcaacgcac gttgatggtc tgccctaatc gctgattcgc
    23881 agaggtccct atgttcacgg caacagaggc agtcccggta gcaaaagtgg tggcaggaaa
    23941 taagcgttac cccggcgtcg ttgcgctgga taacgttaac ttcacgctca ataaaggcga
    24001 agttcgtgcg ctgttaggta aaaacggcgc gggcaaatcg actctcattc gaatgcttac
    24061 cggcagcgaa cgcccggata gcggtgatat ctggattggc gagacgcgac tggaaggtga
    24121 cgaagctacg ttgactcgcc gtgccgctga actgggggtt cgtgcggttt atcaggaatt
    24181 aagtctggtg gaagggctga cggtggcaga aaacctctgc cttggtcaat ggcctcgccg
    24241 caacggcatg attgattacc tgcaaatggc gcaggatgcc caacgttgct tacaggcgct
    24301 gggcgttgac gttagtcctg aacaacttgt ttcaacgcta agcccggcgc aaaagcaact
    24361 ggtggaaatt gcacgggtga tgaagggcga gccgcgcgtg gtcattcttg atgaacccac
    24421 cagctcgctt gccagtgcag aagttgaact ggtgatcagt gcggtgaaaa agatgtcggc
    24481 actgggcgtg gcggtgattt atgtcagcca ccggatggaa gaaatccgcc gcattgcctc
    24541 ctgtgccacc gttatgcgcg atggtcaggt ggcgggcgat gtgatgctcg aaaacacctc
    24601 cacgcatcat attgtgtcgc taatgctagg gcgtgatcac gttgatattg cgccggttgc
    24661 ccctcaggaa attgtggatc aggccgtact ggaagtccgt gcgttacgcc ataagcccaa
    24721 gctggaggat atcagcttta cgttacgtcg cggcgaagtg cttggcattg ctggcctgct
    24781 gggggcaggg cgcagtgaat tgctgaaggc gattgttggg ctggagacgt atgaacaggg
    24841 cgaaattgtt atcaacggcg agaaaatcac gcgccccgat tacggcgaca tgctgaaacg
    24901 cggcattggc tatacgccag aaaaccgcaa agaagcgggg atcattccct ggttgggcgt
    24961 tgacgaaaat acagtgctga ccaatcggca aaaaatcagc gccaacggag tgctgcaatg
    25021 gtccaccatc cgccgcctga ccgaagaggt gatgcagcgg atgacggtca aggccgctag
    25081 tagcgaaacg cccatcggca cgctttctgg tggcaatcag caaaaagtgg tgatcggtcg
    25141 ttgggtctat gccgccagcc agattttgtt gctcgacgag ccaacgcgcg gcgtcgatat
    25201 cgaagccaaa cagcagattt accgtattgt ccgtgagctg gctgccgaag gaaaaagcgt
    25261 ggtgtttatc tccagtgaag tggaggagtt gccgctggtg tgtgaccgca tcctgttatt
    25321 acagcacggc acgttctcgc aggagtttca ctcgccggtc aatgtggatg agctgatgtc
    25381 cgccattctg tctgtgcact gatgaatttt accgaggaac ctgaagatgt ctgcttcgtc
    25441 attaccattg ccgcagggca agagcgtctc gctcaaacaa tttgtcagtc gccatattaa
    25501 tgagatcggt ttgctggtgg tgattgccat tctttatctg gtcttctccc tgaacgcgcc
    25561 tggctttatc tcattgaata accagatgaa cgtgctgcgc gatgccgcca ccattgggat
    25621 tgccgcctgg gcgatgacgc tgattattat ctccggtgaa attgatgtca gcgttgggcc
    25681 gatggtggct tttgtctcgg tgtgcctggc atttttgctg caatttgaag ttccgttggc
    25741 gattgcttgt ctgctggtgt tgctgttagg cgcgctgatg ggaacgctcg ccggggtgct
    25801 gcgcggcgtg tttaacgtac caagtttcgt tgccacactg gggctgtgga gcgccctgcg
    25861 cggaatgggg ctgtttatga ccaacgcact gccagtgccg attgacgaaa acgaggtgct
    25921 ggactggctg ggcggacaat ttctcggtgt gccagtatcc gcgctgatca tgatggtgtt
    25981 gtttgcgctg tttgtgttca ttagccgaaa aaccgccttc gggcgctcgg tttttgccgt
    26041 tggcggtaat gccacggcgg cgcagttgtg cggtattaac gttcgtcggg tgcgcattct
    26101 tatctttacc ctttcgggat tattagcggc ggtgaccggc attttgctgg cggcacgcct
    26161 cggttccggt aacgcaggtg ccgcaaacgg tctggagttt gacgtcatcg ccgcggtcgt
    26221 ggtcggcggt acggcacttt ccggcggtcg cggttccctg ttcggtacat tgcttggtgt
    26281 gctggtgatt acgctaatcg gtaacggtct ggtgctgctc ggtattaact cctttttcca
    26341 gcaggtggta cgcggcgtca tcatcgtggt ggcggtgctg gcgaatatct tgctgaccca
    26401 gcgaagcagt aaagcgaaac gctaaacctt aacgcccctg gcgcgggtgc ggcagggatg
    26461 aaaattaatc gtattgttaa tgagggattt atgaaaacga tgctggcagc ttatttacca
    26521 ggaaattcaa ccgtcgatct gcgggaagtt gcggtgccga cgccggggat taaccaggta
    26581 ctgatcaaaa tgaaatcctc cgggatttgc ggaagcgatg tccactatat ctatcatcaa
    26641 cactgtgcca cggcggcggc acccgataaa ccgttatacc agggatttat caacggtcat
    26701 gaaccgtgcg ggcagattgt ggcgatgggg caaggctgcc gccattttaa agagggcgac
    26761 cgcgtgctgg tgtatcacat ttctggctgt ggtttttgcc cgaactgccg ccgcggcttt
    26821 cctatctctt gtactggcaa aggaaaagcg gcttacggct ggcagcgtga cggcggtcat
    26881 gccgaatatc tgctggcgga agaaaaagat ctgatcctcc tgccggatgc gctgagctac
    26941 gaagatggtg cgtttatcag ttgcggcgtt ggcacggcct atgaagggat tttgcgcggc
    27001 gaagtttccg gcagcgataa cgtgctggtg gtcggtctgg ggccggtcgg catgatggcg
    27061 atgatgctgg cgaaaggtcg cggtgcaaaa cggatcatcg gcgttgatat gctgccggaa
    27121 cgtctggcga tggcaaaaca gttaggggtg atggatcacg gctatttagc aaccaccgaa
    27181 ggtctgccgc agattatcgc cgaactcacc cacggtggcg cggatgttgc gctcgattgt
    27241 tctggtaatg ccgcaggtcg cttgctggca ctgcaatcca ccgctgactg gggacgggtg
    27301 gtttacattg gtgaaaccgg aaaagtggaa ttcgaggtca gcgccgatct gatgcaccat
    27361 caacggcgga ttatcggctc ctgggtgacc agtctgttcc atatggaaaa atgcgcccac
    27421 gatttgactg actggaagct gtggccgcgt aacgccatta cccatcgctt ctcgctggaa
    27481 caggcaggtg atgcctatgc gctgatggcg agcggcaaat gcgggaaagt tgtgattaac
    27541 ttcccggatt aaggagttag cggatgacca tctatacctt atcccacggg tcgctaaagc
    27601 tggacgtttc tgaccagggc ggcgtaatcg aaggcttctg gcgcgatacg acgccgctct
    27661 tacgtcctgg taaaaaaagc ggtgtggcaa ccgatgcctc atgttttccg ctggtgccat
    27721 ttgcaaaccg ggtgagcggc aaccgttttg tctggcaggg gcgtgagtat caactgcaac
    27781 cgaatgttga gtgggatgcg cactatctac acggcgatgg ctggctgggc gagtggcaat
    27841 gtgtctcgca cagcgatgat agtttgtgtc tggtgtatga acatcgcagc ggtgtctatc
    27901 actatcgggt aagtcaggcg tttcatttaa cggcggatac gctgacggtg acgctctctg
    27961 tcaccaatca aggggcagag acgctgccat ttggtaccgg ctggcatcct tattttccgt
    28021 tgtcaccaca aacgcggatt caggcgcagg cgagcggtta ctggctggaa cgggagcagt
    28081 ggctggcggg tgagttttgc gagcagctac cgcaggaact ggattttaac cagctcgcgc
    28141 cgttgccgcg ccagtgggtg aacaatggtt ttgccggatg gaatggtcag gcccgcatcg
    28201 agcagccgca ggaaggttat gccatcatca tggaaacgac gccacctgca ccgtgttatt
    28261 tcatctttgt ttccgaccct gcgtttgata aaggatatgc gtttgatttc ttctgtctgg
    28321 aaccgatgag ccatgcgccg gatgatcatc atcgcccgga agggggtgat ctcattgcgc
    28381 tagcaccagg ggaatcaaca acttcagaga tgtcgttgcg ggtagagtgg ctgtaattta
    28441 ttaaatgccg gataaggcgt tcacgccgca tccggcatct ttcctaacgc cgatcgagag
    28501 aaattgcccc cggcccggta attgccaata gcaagaacgc gccagcgata ctgacattct
    28561 tccagaaatt aatcatattt ggcccaaccg catcgccggt catatcccag taatggtgac
    28621 caatcaccgc cgtacccagc gtgtagaaaa taaacagcac cgccagcgga cgggtgaaaa
    28681 agccaagcac gattaatatc gcggcgggca cttccataac taccgcaata atcgctgcca
    28741 gcatcggcat tggcgcgccc aacgaggcca tatattggac cgtaccgtca aagcccatca
    28801 ttttgggaaa accaaaaata atgaaaatta agaccacggc gatacgggca attaataaca
    28861 aaacggggcg ggcagctcca aaatcaaaat aacg
//
LOCUS       DAZPEP010000040        28601 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZPEP010000040 DAZPEP010000000
VERSION     DAZPEP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28601
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.040"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUT9_001709"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="ACHUT9_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFR1348207.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUT9_001710"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="ACHUT9_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFR1348208.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUT9_001711"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="ACHUT9_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFR1348209.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUT9_001712"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="ACHUT9_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFR1348210.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUT9_001713"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="ACHUT9_001713"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFR1348211.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUT9_001714"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="ACHUT9_001714"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFR1348212.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="ACHUT9_001715"
     CDS             complement(6574..7338)
                     /locus_tag="ACHUT9_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFR1348213.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUT9_001716"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="ACHUT9_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFR1348214.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="ACHUT9_001717"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="ACHUT9_001717"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFR1348215.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="ACHUT9_001718"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="ACHUT9_001718"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFR1348216.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="ACHUT9_001719"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="ACHUT9_001719"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFR1348217.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="ACHUT9_001720"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="ACHUT9_001720"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFR1348218.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUT9_001721"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="ACHUT9_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFR1348219.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUT9_001722"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="ACHUT9_001722"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFR1348220.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUT9_001723"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="ACHUT9_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFR1348221.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUT9_001724"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="ACHUT9_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFR1348222.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="ACHUT9_001725"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="ACHUT9_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFR1348223.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUT9_001726"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="ACHUT9_001726"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFR1348224.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUT9_001727"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="ACHUT9_001727"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFR1348225.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUT9_001728"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="ACHUT9_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFR1348226.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUT9_001729"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="ACHUT9_001729"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFR1348227.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUT9_001730"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="ACHUT9_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFR1348228.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..>28601)
                     /locus_tag="ACHUT9_001731"
     CDS             complement(27550..>28601)
                     /locus_tag="ACHUT9_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HFR1348229.1"
                     /translation="LLGLFFIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIA
                     RPLQVPNKQRRWFAVLLGQGSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPI
                     LLVILNRLEQSSTEEAREADEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHD
                     PDHIETLRKFGMKVFYGDATRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFP
                     HLQIIARARDVDHYIRLRQAGVEKPERETFEGALKTGRLALESLGLGPYEARERADVF
                     RRFNIQMVEEMAMVENDTKARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGK
                     HTGNMADEPETKPSS"
BASE COUNT         6780 a         7855 c         7598 g         6368 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aaaaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggcg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caggagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaacgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtctg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatctcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgccg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaggca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc a
//
LOCUS       DAZPEP010000041        28050 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZPEP010000041 DAZPEP010000000
VERSION     DAZPEP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.041"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..248
                     /gene="tnpA"
                     /locus_tag="ACHUT9_001732"
     CDS             <1..248
                     /gene="tnpA"
                     /locus_tag="ACHUT9_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFR1348230.1"
                     /translation="MDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRPDIAV
                     RYYYKGVLWSPGYFANSCGGAPISVIRQYIEQQQTPG"
     gene            308..382
                     /locus_tag="ACHUT9_001733"
                     /pseudo
     CDS             308..382
                     /locus_tag="ACHUT9_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(379..1702)
                     /locus_tag="ACHUT9_001734"
                     /pseudo
     CDS             complement(379..1702)
                     /locus_tag="ACHUT9_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1702..3318)
                     /locus_tag="ACHUT9_001735"
     CDS             complement(1702..3318)
                     /locus_tag="ACHUT9_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFR1348231.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3614..4330
                     /locus_tag="ACHUT9_001736"
     CDS             3614..4330
                     /locus_tag="ACHUT9_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1348232.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4327..5988)
                     /locus_tag="ACHUT9_001737"
     CDS             complement(4327..5988)
                     /locus_tag="ACHUT9_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFR1348233.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHUT9_001738"
     CDS             complement(6184..6612)
                     /gene="ibpB"
                     /locus_tag="ACHUT9_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFR1348234.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHUT9_001739"
     CDS             complement(6724..7137)
                     /gene="ibpA"
                     /locus_tag="ACHUT9_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFR1348235.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7131..7203)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7443..7775
                     /locus_tag="ACHUT9_001740"
     CDS             7443..7775
                     /locus_tag="ACHUT9_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1348236.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7777..8991)
                     /locus_tag="ACHUT9_001741"
     CDS             complement(7777..8991)
                     /locus_tag="ACHUT9_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFR1348237.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHUT9_001742"
     CDS             9092..10156
                     /gene="cbrA"
                     /locus_tag="ACHUT9_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFR1348238.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10153..11490)
                     /locus_tag="ACHUT9_001743"
     CDS             complement(10153..11490)
                     /locus_tag="ACHUT9_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1348239.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHUT9_001744"
                     /pseudo
     CDS             complement(11565..12713)
                     /gene="dgoD"
                     /locus_tag="ACHUT9_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHUT9_001745"
     CDS             complement(12710..13327)
                     /gene="dgoA"
                     /locus_tag="ACHUT9_001745"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFR1348240.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHUT9_001746"
     CDS             complement(13311..14189)
                     /gene="dgoK"
                     /locus_tag="ACHUT9_001746"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFR1348241.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHUT9_001747"
     CDS             complement(14186..14875)
                     /gene="dgoR"
                     /locus_tag="ACHUT9_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFR1348242.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15153..15809
                     /locus_tag="ACHUT9_001748"
     CDS             15153..15809
                     /locus_tag="ACHUT9_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348243.1"
                     /translation="MKLNLKGFFKTAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLKDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYNWSTEEEKARVTQYRFACGVQHCTRTVENLEGTIHEKNKN
                     MDYSNVMAFYHPFKVRFYEYHSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHUT9_001749"
     CDS             complement(15855..16667)
                     /gene="yidA"
                     /locus_tag="ACHUT9_001749"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFR1348244.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16782..17180)
                     /locus_tag="ACHUT9_001750"
     CDS             complement(16782..17180)
                     /locus_tag="ACHUT9_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFR1348245.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHUT9_001751"
     CDS             complement(17420..19834)
                     /gene="gyrB"
                     /locus_tag="ACHUT9_001751"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFR1348246.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHUT9_001752"
     CDS             complement(19863..20936)
                     /gene="recF"
                     /locus_tag="ACHUT9_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFR1348247.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHUT9_001753"
     CDS             complement(20936..22036)
                     /gene="dnaN"
                     /locus_tag="ACHUT9_001753"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFR1348248.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHUT9_001754"
     CDS             complement(22041..23444)
                     /gene="dnaA"
                     /locus_tag="ACHUT9_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFR1348249.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23741..23821
                     /locus_tag="ACHUT9_001755"
     CDS             23741..23821
                     /locus_tag="ACHUT9_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348250.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHUT9_001756"
     CDS             24051..24191
                     /gene="rpmH"
                     /locus_tag="ACHUT9_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFR1348251.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHUT9_001757"
     CDS             24208..24567
                     /gene="rnpA"
                     /locus_tag="ACHUT9_001757"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFR1348252.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24531..24788
                     /gene="yidD"
                     /locus_tag="ACHUT9_001758"
     CDS             24531..24788
                     /gene="yidD"
                     /locus_tag="ACHUT9_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFR1348253.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24791..26437
                     /gene="yidC"
                     /locus_tag="ACHUT9_001759"
     CDS             24791..26437
                     /gene="yidC"
                     /locus_tag="ACHUT9_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFR1348254.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHUT9_001760"
     CDS             26543..27907
                     /gene="mnmE"
                     /locus_tag="ACHUT9_001760"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFR1348255.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6760 a         7571 c         7159 g         6560 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gttactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtcctggc tattttgcca
      181 atagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccgc gccttatatc cccgccctga gggacggggt tttacggcgc
      301 accggataaa tttggttatg cgccgtcagc cgtgctaaag aatacggacc agcacccaac
      361 ggatgccagt ccacacaatt aacgtaactc tggccagtac gctttgttgg cctccagcaa
      421 ctcatccaga atagctttgg cgaccgaggc gctcggtacc gttttcgata acgtaatcgc
      481 ctgccacagg tgctgatatg aacgctgttc ccaggcgtcc acgaccagtt tttccaccgc
      541 cacttgctga ctcattaacc ctttctgaaa ctgcgggata tcgccgacca ccagtggttc
      601 tggtccgttg tgcccgacca gacacgggat ctcgaccatc gcttcatcat caaagttatg
      661 gattgccccg ttgttaggca caatcagcaa catccgctcc tgagtgttga aggcaattgc
      721 cgccgccaga tcgacgatgt atgacgcatg ttcgtcaatc tccagcttgg ccgccgctga
      781 atttcctgcc gctgtaatgg cgcggcaagc atcgaaaacc tgtttctcgc gatgttccat
      841 tcacctcatt cgcgcgggta tgttcagggt tggaatgctg aacaacataa tccgggaaga
      901 gataatattt cagataggtg ttcggcaatg tatccgggtc cagtgcctgg acatcccgcg
      961 cttttgcata ggtgtcgttc cagctcgctt cagtatgtgt atcttgctgc ggtggaacat
     1021 aaccgtgctt cgagacatat tgccgcagct ggggcatcag gtcgttgccc tgcaaatctt
     1081 caattgatgt ccaccagcca aagtggttca ggccgtagta gcgcacgcgc atctgtttgc
     1141 gatcttgcag cccaacaatt tgcgccatcc ggctttcaat accgattggc atgtcacaga
     1201 tgttgaggat tttcgcattc gggcgcagac gacgcgtggc ttctgcgaca atggctgccg
     1261 ggttggagta gttgagcatc caggcatttg gtgaatattt ttccatataa tccaccagtt
     1321 ccaggacgcc gccaatggaa cgcatgccgt acgctattcc gccgggtccg caagtttcct
     1381 gaccaacaac accgtggcgc agtgggattt tttcatccag ttcgcgcatc gggtacttgc
     1441 cgacgcggat gtgcgccatg acaaaatcaa cgtcgctgaa cgccacttca ggatcggtgg
     1501 tgtaactaaa cgcaatgtcc ggcgctttct ctttaaggat gactttacag gcttcggcaa
     1561 tcacttcctg ccgcgcgcca tcgttatcat aaaatttcag tgcacgaagc gggaaacgct
     1621 cctgattcgc caggagcatc aacacgatgc ctggcgtaaa ggtgcttcca ccgcctgcga
     1681 caaccactga gaatttggtc attatactgc ctccgtaatg gcaacatttt ctgctgattg
     1741 atgagaatta attaagctat cgagctgttc acgcagctgg gatacatgca gaccaatgat
     1801 cacctgaatg gcatcgccac tacggaagac gccgtgcgct cccagctttt taaagacttc
     1861 gtcatccagc gtttgtgaca tgtcatgcag tgcaatgcgt agacgcgtcg cacaattgtt
     1921 aatgctggag atattgccga ccccgcccag ggcttgcagg ataccggcag cttgatccag
     1981 ctcttttttt ggctctgccg cggtggtttg gcctcgcgag gctttgtatt cggcttttga
     2041 gtagagtttc acttccgcat cttcacgtcc cggcgtgcac atgttgaact gcagaatcag
     2101 ggtgcggaaa accacgaagt acagcagggt aaagcacaac ccaatggcga tttgggtcag
     2161 catcatatcc gcgtggttgc tgaacatcgg gatccagttt tgcggtaaaa cctggtcaat
     2221 cagacctccg cccatgttgc ccaccacacc aaagagatac atcacggtcg acattgaggc
     2281 cgcaagcaca gcgtgtaccg caaacagcaa cggtgaaatg aacaggaagg taaattccag
     2341 cggttcggta attcccacca gcatggcggt taaggttgcg ggaatcagca agcccgccac
     2401 ttttacccga ttttccggtg cggcagtgaa gtacatcgct aaagaaatgc ccacggcacc
     2461 aaagattttt gagttaccgt gcagggcaaa accgccttcc gggaacaacg atttcagcgg
     2521 ctcggcgctc aaactgaact cttgcagatg ctgcgcccag tacatctgaa tgccgccttc
     2581 aacagctgcc ggacccaaga taaacggtcc gtagatgaag tggtgtaaac cggttgggat
     2641 cagaatacgt tcgaggaagg tgtaaaccca gaccccaagc gcacccgccg aacgcaggaa
     2701 cgcttgcaga gattcaatcc ccgtttgtac ttttggccag ccgagcaatg tcagccaggc
     2761 acaggggatc atcaccagga aggcgataat caccacataa gacgtcccct ggaaaatgcc
     2821 gagaaaaacc ggcagttttt tatcgaacag acggttatgc agcgccgtca caatgccgga
     2881 aatgataatt gcgccgataa tgctggtatc gagggtttta atcccggcca tcattgtcag
     2941 accgctacct gccaccgcgt cctgagtgaa atcgacgccg aagtagcttc cccaggtcat
     3001 tcccatcgcg ttgatgaaat agttccaggt caggaaactc accatcaccg ccagacaagc
     3061 acgtccctgc gcttgcttag caaggccaat gggtaaaccg acagcaaaaa tcagcggcat
     3121 attacggaat accgtccaac cgccctcttc aataatgtgt acgatttgcg cgaataaact
     3181 gttcggatcg gtcagtgatt ccccgacaaa catcgggttt tgcagcaaga tggcaagacc
     3241 caccacaatc ccggcgaagg gaaacagcag cactggcgta aacatcgcgc cgccaaagcg
     3301 ttgaatttga ctgagcattt ccacatcctc ttttctcaat tctgtaagta ggaagcctga
     3361 ttggttatgc ggcaggcctg gagcaaggat aggaagatgc gatcggcaga acatgacgac
     3421 ccaatgggat tcgtgatcgc tttcatgctt ttcgttgggc ggtagagatc tacgttttcg
     3481 cgaaaaagta gacatctgtg agcacaggta cacagcatcg aacacatctt taaaaaaaag
     3541 atgttttttc aatcgattaa gcagaacttg tgtgcgcatt acccgggctt gcaggcaaaa
     3601 aagagatcta gagatgatct acaaaagcat tgcggagcgg ttaagaattc gacttaactc
     3661 cgcagatttc acgctaaaca gccttcttcc gggtgaaaaa aagctggcgg aagagtttgc
     3721 ggtatcgcgg atgaccatcc gtaaagccat tgacctgctg gtagcgtggg ggctggtggt
     3781 ccgccgccac ggtagtggca cttacctggt gcgcaaagat gtactgcatc aaaccgccag
     3841 tctgaccggg ctggtggagg tgttaaaacg gcagggaaaa actgtcacca gccaggtgct
     3901 gatttttgaa atcatgcctg cgcctccggc cattgccagc cagttacgga ttcaaatcaa
     3961 cgagcagatc tacttctccc gtcgcgttcg ttttgtggaa gggaaaccgc tgatgctgga
     4021 agacagctat atgccggtaa aactgttccg taatctttcg ctgcaacatc tggaagggtc
     4081 gaagtttgaa tatattgaac aagagtgcgg gattttgatt ggcggtaatt atgaaagcct
     4141 gacgccggtg ctcgccgata gactgctggc gcggcaaatg aaggtagcgg aacacacgcc
     4201 actgctgcgg atcacctcgt tgtcatatag cgagagcggg gagtttttga attattcagt
     4261 gatgttcaga aatgccagcg aataccaggt ggagtaccat ttacggcgac tccacccgga
     4321 aaagagttaa ccgatactcc agaagagcac cgccagtaat tggggggtga taatgcgcag
     4381 gaacatcact aacggataga cagtggcgta agagagcgcc gccgcaccgc tagttggatg
     4441 aagattatta gcaaacgcca gcgccggagg atcggtcatg gaacctgcca gcatcccgca
     4501 catggtcagg taattcattt tggctaacat ccgcgccaga atgccaacag taatcagcgg
     4561 aacggcggtg atcagggcac cataaccaat ccagcttagc ccttcgccat tgaccagggt
     4621 attcacaaaa tccccaccag atttcagacc aacgaccgag aggaacagca cgatccccag
     4681 ctcccgcagc gcgaggttgg cacttggcgg cataaaccag tacagcttgc cgatactgcc
     4741 gatacgcccg aggatcaacg ccataatcag cagaccgccc gccagcccca gtttcaacgc
     4801 ggccgggaat cccggcacaa agacgggaat agaacctaac aatacgccaa gcccgatgcc
     4861 aataaacact ggcagcatct gaacctgttg cagtttttgc tgcgcattcc ccagcacatt
     4921 ggcaacggca tcaattgcgg acggacgccc caccaggttg aggatatcgc cgaactgcag
     4981 gctgatatcg ccactggcga ccagttcgac cccggcacgg ttcaggcgcg agataacaac
     5041 gtcatagcgt tctttaaagt gcaggtcgcg aatacgtttt ccgagcacgt tttcattggt
     5101 gaccaccaca cgctcgacgc gcaaatcagt gtctttcgtg gacagcgaag tatcgacctc
     5161 ctgaccaatc accagttgcg cattatgtaa atccgctggc tgacccacca gatgcagcaa
     5221 atcgcccagt tggataatgg tatctggcga aggaactttt agggtttctt cgcgtttcag
     5281 acgcgagcag ataattttgt cgccgttgag aatcggtaca tctttaatgg ctaaatcatg
     5341 caggttaggg ttctcaacgc gaatattgat agtcttgatc agcgcgccgc cattggtgcg
     5401 tgaagactcg tgctgctgag cttctgtctc gacattgacg cggaaaataa cccgcaacat
     5461 ccacatggtg aacaaaatcc cgcaaatgcc gaatggatac gccatcgcgt aactcatccc
     5521 catctgatcg accatttcca ttggtgtacc caggtcgcgc aaaatctgct gccctgcccc
     5581 cagcgctggc gtattggtaa ccgcaccgga gaaaatcccc agcactaccg gcagtggaat
     5641 atcaaacagt ttatgcagga tggcggtaac cagaccaccg atgatgacga tcagaacagc
     5701 aaacaggttg aggcgtaatc cggagacgcg caatgaggca aagaagcccg gccctacctg
     5761 aatcccgatg gtataaacga acaggatcag gccaaattcc tgaataacat gcagcatatc
     5821 gctacttaat gtcatccccg cctgagaaac aaaatggccg acgatgatcc caccaaacag
     5881 cacgccgcca atacctaatc ctatgccgcg aaatttgacg ttgccgataa acaaaccgac
     5941 gactgccacc aaagccagaa tactgaccgt taatgctata tcactcatcg tccatttctc
     6001 ttgagataac attttaattg ctacggatta tggctgaatc ggtgaagcag atttgcctta
     6061 tagcgcacat tattacggga aagaacgtgc cgaaatatct taaacagtcg aaatttaacg
     6121 cattgttgaa gaagaaagac aaaaaaagcc ccaccagaat ggcggggcaa agagaatagc
     6181 tagttagcta tttaacgcgg gacgttcgct gatagcgata cgctgcgctg cgatgggttc
     6241 aggctcatta cgaattaaat caatatgcag taaaccgttt acgaaagttg cgccagagac
     6301 ttccatattt tcagccagcg taaagctcag gctaaatggc tgattcataa gcccttgatg
     6361 cagccatttt ttctcttctt ttggctgctc cggcgtgcct tttacgctca ggcgcgtacc
     6421 ttccagttga atctctaaat cttcctgacg gaaacctgcc agcgcaaggg taatgcggta
     6481 gtggttatcg tcgcttttct caatgttgta cggcgggaag ctctgacttt caccggcgtt
     6541 ttgcagtgcg ttggccagtt tgtcaaaacc gatccattga cgcatcagtg gggataaatc
     6601 gaagttacgc atagtcattt ctccttctaa gaagcgagta agtacctgca aatccgaaga
     6661 ttcgcatatg ctccctgacg gcgagcatgg agatgtcagg ccgcgccagg cggccttaag
     6721 gaattagttg atttcgatac ggcgcggttt tttcgcttcc ggaatcacgc gttcgagatc
     6781 gatatacagc aaaccattta ccaggttagc gccacgaaca tgaatgttct cagctaactg
     6841 gaatttgcgt tcaaagttgc gttcagcgat gccctggtac agataggtgc gctctttttg
     6901 ttcgtcggcg tgagcacctt tcaccaccag cagattatcc tgggcggtaa tttccagttc
     6961 gctctcagca aaaccagcca cagcgatagc aatgcggtaa tggttttcgt ctaccagttc
     7021 aacgttatac ggagggtagc cgccattact ctggctctgg ttgttttcta agtggttaaa
     7081 caaacggtca aatccaatag cagaacggta aagcggggat aaatcaaagt tacgcataat
     7141 caatagctcc tgaaatcagc gagaatgtaa gaccttccac aatggacagg tcaggtagcc
     7201 agaacaccca tcaggcgcgt cctcatcgac tacgatgtaa aaatgggtct ggaaatgaac
     7261 ttttcaagcc ttatcagcgg actttttttt gcagtttatg gtctattgca tagactgagg
     7321 gggcagcacg aaacgttaaa gtgcgatagc cgccacagag cgacgtaatg atgatgttat
     7381 tttcagcaag ggatcgctat aactcatcag gccaacaccc acgatcaaca gatgaattta
     7441 ttatgataag aaatgtgttg ttagcgttca tgatatgcag cggaatggca ttactcggag
     7501 gatgctccag cgtgatgtcc cataccggag ggaaagaagg aacgtatccc ggcacccgcg
     7561 ccagcgccac catgattggc gatgatgaaa ccaactgggg cactaagtcg ctggcgattc
     7621 tcgatatgcc gtttaccgct gtgatggaca cgcttttatt accgtgggat gtgttccgca
     7681 aagacagctc ggtgcgctca cgcgttgaaa aaagcgaagc caacgcgcaa gccaccaatg
     7741 cggttattcc accggccaga atgcctgata actgatttag cgatcagttt cggtgatcca
     7801 cagctggcca ccttccatcc acgccagcca ttgaccatcc ggcgagaaga ccacggcgtc
     7861 cgcagaaggt ggattggcgt ggttttcggt taaatactca acctcgccgc tttgcgcatg
     7921 ggcacaagca attcgattat ccagcacaaa gcccaaccat tctcctgacg gatgccagtt
     7981 aaatgcagac tgaatatccg ttttgttatg ggttaactgg cgcggctcgc cgccctgtgg
     8041 cgagataagc cacagttgca caatgccgtt atcatcacgc attaaaaacg cgatttgcgt
     8101 accctgcgga ttacagcgca cccagtggcg cgggacgttg actaaccccg gataagcccg
     8161 atggtgggta aaggttaaac gtcgctgcac gacgccacgc ggtggcgcgg gcagggtggt
     8221 ttccgttcca cttaacggcg catcacctgc cgctttccag ccagcttcat cttgcggcaa
     8281 ctcaacgata aacagctccg gcactttctc accctttggc gaaagtgtgt cgccaataaa
     8341 cgccagcgcg tgatttccta cccatccttc ttcataagca cgattgattt catcactgcc
     8401 aggctgcggc gtgggcgtgg ttttactcac cagcacacac cagtggctgc cgctgtattc
     8461 acgcggatgc tgcttttgta cgttgaccgg accaaacggc gcagcaacac cgacgtttcg
     8521 caaatccagc gccggatcga gttcatgcat tacatggtcg ttataggtaa agctcaccct
     8581 ttcaccgttc gggctaaaga catgcacatg gctgccgccg cgcagcgctc ctggcgtata
     8641 cggcgcggta atatccattg catcgagatt gctcaccttg ccgccttcag caatgactcc
     8701 gcgccgatga tggaagtcgt aatgccatgt ttcatcagga ttttccgggc cgtgaataaa
     8761 gacatatttc tctgacttcg gatgaacggt caccacgccg acgtgtgcgc cctgtgacgc
     8821 acagtagata acctcgacct caccggtatg gatattcaca cgctcaatgg tctcgccggt
     8881 aaacgacgcc ccagaaggac gcacgtcaaa taccagccac tggctgtcgg gcgtccaggt
     8941 attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca tggcgcggtc
     9001 ctgatacaaa acgggtccag ccatcatact tcacaaggac ttcactttca gacaatagtc
     9061 ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg cgattattgg
     9121 cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc aggtgatcgc
     9181 gctggataaa aagcaccagc atggtactga aggtttcagc aaaccttgtg gcggtctgct
     9241 ggcaccggac gcgcagcgat cttttattcg cgatgggctg acgcttcctg tcgatgtgat
     9301 cgctaatccg cagattttca gcgtcaaaac cgtcgacgtc gccgcatcgc tcacgcgtaa
     9361 ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga tgaaatcgct
     9421 gatccccgcc agcgttgagg tttatcacga cagcctgtgt cgaaaaatct ggcgtgagga
     9481 tgataaatgg catgtcattt ttcgtgcaga cggctgggag cagcatatta ccgcccgcta
     9541 tctggtcggt gccgatggtg ccaactcgat ggtgcggcga catctctacc cggatcatca
     9601 aatccgtaaa tatgtcgcaa tccagcagtg gttcgcagag aaacatccgg tgccgttcta
     9661 ctcctgcatc tttgataatg cgataactga ctgttattca tggagtatca gcaaagacgg
     9721 ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt tcacgactct
     9781 gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa gcgaaaaatg
     9841 cacggtgctg tttccctcac gctggcagga ttttgtctgc ggtaaggaca acgcctttct
     9901 gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta gctatgcgct
     9961 ggatagcgca gacattctgc gttcggtgtt actgaagcag ccagagaagc tcaataccgc
    10021 ttactggcgc gccacccgca aactgcgttt aaaactcttc ggcaagatag taaaaagccg
    10081 atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg cacatattcc
    10141 acagttgaag gattagccaa cgcgcttcac atcgcccacc agcaggatat aagagagcgc
    10201 gccaatcaac gcgacggcgg agatataaac cagtgcaggt gcgaaaccgt aaccctgcgc
    10261 caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga agttaaacac
    10321 gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gaaaccagcg accaggtaat
    10381 cgaagcaaaa ccgttaccga agaatgccag cgccatcagg cacataatca tcatcggatc
    10441 gttagtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga taatcggcgt
    10501 tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcta cccagccaga
    10561 gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc ctgctttcag
    10621 cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga aaaaccacag
    10681 tgtagaagcc accgcaaatt gcccaagata gacgccgatc agtttacgat ggaacaccag
    10741 tttccagtct ttggctgtta acggctgacg tgcctctttc ttcactggcg catcaccatc
    10801 caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatgc ctttggtcag
    10861 gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga taccaccagt
    10921 cacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca gcggagtcaa
    10981 aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag cgcgttcatg
    11041 ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg cttcgaaaat
    11101 accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc cctggaataa
    11161 agtcgccact gaccagccaa atatcgcaat aaaataagtc acgcgagaac ctacgcgatc
    11221 taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg ccgaaaatac
    11281 atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg aagccacggc
    11341 caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca ccagcgtcag
    11401 ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga gctttctcca
    11461 gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg ttatattttt
    11521 gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca ctctgctacg
    11581 ctgttatctt catgacgcca gagtggatta cgccagtccg gggcattttt actgaactca
    11641 ataactttag cttcgtcgat ttccacgcct aagcccggtt tcgttagcgg tttaaagaag
    11701 ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc cgcgcctttg
    11761 ttgtaatgaa ttcccatact ttgttcctga agtaccgcgt tataggaaac aaagtcgata
    11821 tgcagacaag ccgccagtgc aatcggtccg agcggacagt gcggcgcaag ggtcacgtca
    11881 taggcttctg ccattccggc aattttgtag cattcggtaa taccgcctgc atgggagaga
    11941 tccggttgta gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc gaaacgtgag
    12001 aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt cgggtagtat
    12061 tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg ctccagttct
    12121 ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag accaaactca
    12181 atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc atctaccgcg
    12241 cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt atcgaagccg
    12301 atttcgcgca gggttttaat accgtcgatc acatccgccg gacgatcgcc gccgacccaa
    12361 ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac cgacgcattc
    12421 agcactttac ctttgatatc ccataacgcc tggtcgatcc cggcaatggc gctcatcagg
    12481 attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc attgatgcgc
    12541 tatggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc caccgtgcgg
    12601 gcgcggcctt caatcactgg ctcgccccag ccgacgacgc cttcatcggt ttcaattttc
    12661 aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt cattgcactg
    12721 cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct acggactgcc
    12781 cggcgcgata gagatcgctg cctaagcccg ccccagcaca acctgcgtct atccactgcg
    12841 ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccgat ggcaataccg
    12901 cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt ttcagcgcct
    12961 gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg gggcagacgg
    13021 tcatgccgta gcccaccgca cggcggatca cttcactatg gatattgggc gtaacgatga
    13081 gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc gtacctgcgc
    13141 caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctttgc tcccattgtg
    13201 gggaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg ccaacatgcg
    13261 ccagcgcctc gtcgggctta atgccgcgca aaatggcgat cagcggaagt ttagtttgcc
    13321 actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc caccgccgtc
    13381 acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcaacga tgttccggcg
    13441 acaagggtga tggcgtgttg atgggccaca tagtcgcgca tactggcgac ctctgcgcca
    13501 atcaacaaac cagagagaaa ttcactgacc tgttcacggg gaagtgttcc cagcacatgc
    13561 gaggcacgaa cttcaaaaag ctgcggcaat atggcgggcg tattaagacc acgctcaagg
    13621 ccagccgtga aggcatcggc agagttttcc tgcggcggca aacctgcacc aatcaatgag
    13681 tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc gttgatttgc
    13741 tggctatcgg cctgcaccca tttgcaatgc gtaccgggca tgacataaag agaggaagga
    13801 gccagagctc gcgcgccgat caattgagtt tcttcgccgc gcatcacatt gtggttatca
    13861 tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga cgttaattgt
    13921 tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt ccagccgaca
    13981 ttgctgccga ccattcctgc cattaccact ggcgtattct cttcacgcca gtcggtcgtg
    14041 acttctgcta acaccgcagc cggagatttt ccgttcaggc gcgtgacgcc tgcttctgat
    14101 tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt ggtcgatccc
    14161 cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt gtcgagctgg
    14221 cgatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt atcgcatcga
    14281 acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc atccaggttc
    14341 gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt aacaccgggt
    14401 tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta aacgcttcgc
    14461 ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc agatcgctgg
    14521 aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt cgcacttcgc
    14581 tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc agtacgtcag
    14641 tgtcgaggta attccactgg ttacgcggtg ccacaaacgc gccgcgataa cgtttcattt
    14701 caatcagccg tttcgccatc aacgaacgga acacctcacg gatgatgttg cgcgaggttg
    14761 caaactcctc acagagttcc gcctcagccg gaagcggcga gcctggtacg tatttgccgt
    14821 gaacgatctg tttacccagc gtaatgacga tgcgatcggt tttattgaga gtcatggaga
    14881 gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa cacggcaact
    14941 ttgtagtacc agcgtgatga cgttcgcgtt tgctgtgcgt gtaatgtagt acaaacttat
    15001 attgttgtac tacaatttag atcacaaaaa gaacaatgcg taaaaaaatg acatgcgtcg
    15061 ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga gtatgctttc
    15121 aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaatcttaag ggatttttta
    15181 agactgccgg tttattccca ctggcgctga tgctttcggg ctgtatctcg tatgccctgg
    15241 tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac accatcacgg
    15301 ggctatcgca ggcaaaggat agtaatggaa caaaaggcta tgtttttgta ggggaatcgc
    15361 tggattacct tatcacagat ggtgccgatg acatcgttaa gatgctcaag gatccagctc
    15421 ttaaccggca caatattcag gttgctgatg acgcaagatt tgttttaaat gcggggaaaa
    15481 agaaatttac cggcacaata tcactctatt acaactggag cacagaagaa gaaaaagcac
    15541 gagtgacaca atatcgtttt gcttgtggtg ttcagcactg tacccggaca gtagaaaacc
    15601 tggaaggtac tatccatgag aaaaataaaa acatggatta ctcaaacgta atggctttct
    15661 accatccatt taaagtgcga ttttatgaat accattcacc cagaggcatt ccggatggtg
    15721 tttccgcagc attactgcca gtgactgtta cgctggacat cattactgca ccgctgcaat
    15781 ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt ttatgctgtt
    15841 tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac gccatcttca
    15901 aggttagatt tggtgacaaa gttcgccact tctttcaccg aaggaatagc gttatccatc
    15961 gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc gccaatcgcc
    16021 atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc cgtacctttg
    16081 ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac ggtatatttc
    16141 tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg ttcatcaatc
    16201 atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca gaacaccagc
    16261 ggaatagtgg caacgaaaga ttcatgcact gtgtagtagc tgatatcgcg gttggcggtg
    16321 tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga gagtttttcc
    16381 aggaaacgat agtcgtcata gctgagagca gtttgcgcca cggtgctacc atcagcggcc
    16441 ttctgtacta gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc catatgcagc
    16501 tctttcaggt agttgtgcac acctgcatac gggcgacccg tcgttagcac gacattcacg
    16561 ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt gtgatcgggc
    16621 agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag ttccccaggt
    16681 agattggttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca acacccgagg
    16741 gaaaaggggg atgcaacgcg ggttgcatgc tccccatttg cttagcggaa gagtttccct
    16801 ttcagcagtt ccatacctgc ggaaagcaga tcgttattgg cttgtggtga cacttcacct
    16861 tgcggtgaga gcgcatcaat aatcttcggc aattgctctg ccagtaaact ggaagctgta
    16921 ctggtatcca cgccaagttt ttgcccgaga tcggacaccg catttgtgcc gagcgccgat
    16981 tccagttgct cgccactaac cgattgattg ccctgttgat tactcagcca ggttgagaga
    17041 atggccccta agccgccact ttgcagtttt tccagcagca cctgaatgcc gccctgctcc
    17101 tcaacccaac ttaaaatagc ctgatatttc cccgcatcgc ctttcagaaa ggcaccgaca
    17161 acttcatcaa aaagccccat gataatcacc tgtaaagcgt tacgtgttga cccaaaaagt
    17221 atagatttgc gggatgataa ttgcggattg cagaaataaa aagggcggag atgatctccg
    17281 cccttttctt atagcttctt gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc
    17341 atgaaaattc aatacattgc aagattttcg taggcctgat aagcgtagcg catcaggcac
    17401 gctcgcatgg ttagcgccat taaatatcga tattcgccgc tttcagggcg ttctcttcaa
    17461 taaacgcacg gcgcggttca acggcgtcgc ccatcagcgt ggtgaacaac tggtcggcag
    17521 caatcgcatc tttaacggta acgcgcagca tacgacggct ttccgggtcc atggtggttt
    17581 cccacagctg ttccgggttc atctcgccca gacctttata acgctggatg gagaggccgc
    17641 gacgggactc tttcaccagc cagtccagcg cctgctcgaa gctggctacc ggctgacgac
    17701 gctcgccacg ttcgataaac gcatcttctt ccagcaagcc acgcagtttc tcacccagcg
    17761 tgcagatacg acgatattcg ccaccggtga taaactcgtg atccagcgga tagtcagtat
    17821 ccacaccgtg ggtacgcacg cgaacaatcg gttcaaacag gttttgttcg gcattggtgt
    17881 ggacatcaaa cttccactgg ctgccgtgct gttctttgtc gttcagttcg ctgaccagcg
    17941 cgttcaccca gcgggtaacg gtctgctcat cagagaggtc ggcttccgtc agcgtcggct
    18001 gatagataag ctctttcagc attgctttcg gataacgacg ctccatgcgg ttgatcattt
    18061 tctgcgtcgc gttgtactca gacaccaatt tctctaacgc ttcgccagcc aatgccggtg
    18121 cgctggcgtt ggtgtgcagc gttgcgccat ccagcgcgat agagatctgg tactgatcca
    18181 tcgcttcgtc gtctttaatg tactgttcct gcttgccttt cttcactttg tacagcggcg
    18241 gctgagcgat gtagacgtga ccgcgttcaa cgatttccgg catctgacga tagaagaagg
    18301 tcaacagcag cgtacgaatg tgcgagccgt cgacgtccgc atcggtcatg atgatgatgc
    18361 tgtgataacg cagtttgtcc gggttgtact cgtcacgacc gataccacag ccaagcgcgg
    18421 tgataagcgt cgccacttcc tgagaagaga gcatcttatc gaagcgcgct ttctcaacgt
    18481 tgaggatttt acccttcagc ggcagaatcg cctggttctt gcggttacgc ccttgcttcg
    18541 cagagccgcc cgcggagtcc ccttccacca ggtacagttc ggaaagcgcc ggatcgcgtt
    18601 cctggcagtc tgccagtttg cccggcaggc ccgctaagtc gagcgcacct ttacggcggg
    18661 tcatttcacg cgcgcgacgc gccgcttcac gggcacgggc agcatcgata attttgccaa
    18721 ccacgatttt cgcgtcggtt gggttttcca gcaggtattc tgccagcagt tcgttcatct
    18781 gctgttcaac cgctgatttc acctcagaag aaaccagttt gtctttggtc tgggaggaga
    18841 atttcgggtc cggcactttc acggaaacga ccgcaatcag gccttcacgc gcatcgtcac
    18901 cggtggcgct aactttggct tttttgctgt agccttcttt gtccatgtag gcgttcaggg
    18961 tacgggtcat cgccgcacgg aagcctgcca ggtgagtacc gccgtcacgc tgcggaatgt
    19021 tgttggtaaa gcagtagatg ttttcctgga agccatcgtt ccactgcaac gccacttcga
    19081 cgccaatacc gtctttttca gtggagaagt agaagatatt cgggtggatc ggcgttttgt
    19141 tcttgttcag atattcaacg aacgccttga tgccgccttc atagtggaag tggtcttctt
    19201 tgccgtcgcg cttgtcgcgc agacgaatgg aaacgccgga gttgaggaac gacaactcac
    19261 gcagacgttt cgccagaatt tcatattcga actcggtcac attggtgaag gtttcgaggc
    19321 tgggccagaa acgcaccatg gtgccggttt tttcagtctc gccggtaacc gccaacggtg
    19381 cctgcggtac accgtgttcg tagatctgac ggtgaatttt accctcgcgc tggataacca
    19441 gctccagttt ttgcgacagg gcgtttacta ccgaaacacc aacgccgtgc agaccgccgg
    19501 acactttata ggagttatcg tcaaatttac cgcctgcgtg cagaacggtc atgatcactt
    19561 ccgccgccga tacgccctct tccgggtgaa taccggtcgg aatgccgcgc ccgtcatcct
    19621 gtacagagac agagttatcg gcgtgaatgg tgacgataat ttctttacag tgacccgcga
    19681 gcgcttcgtc gatagcgtta tctaccacct cgaataccat gtggtgcaga ccggtgccgt
    19741 catccgtgtc gccgatatac atacccgggc gcttacgcac cgcatccagc cctttcagga
    19801 ctttgatact ggaggagtca taagaattcg acatcaacgt ttctcgctca tttatacttg
    19861 ggttaatccg ttattttacc cttttccacg gtaaacatct tcgaattttc gtccgacatg
    19921 tctataacgt gttcagcact gatcgcgctg acaaagacct gtgattgcgt cgcttttaag
    19981 cggctggcaa gcagcccgcg acgctcatca tcaagctcag aggcaaaatc atctatcagg
    20041 tagagacacc gccgcccgct ttcacgggtg aggaactctc cttgcgccag acgtaaggcg
    20101 cacatcaaca gcttaagctg cccacgcgat aaggtatctt ccaccggcgc accgtcggcg
    20161 cgaatgcgta agtccgcttt atgcgggcca tgcgcggtgt aggttagctg gcgatcgcgt
    20221 tcaaaattac gttccagcac ctcagcatat tctgtctctt tctcccagcc gcgctggaaa
    20281 gagaaagtca gagaaaactc agggagaaat tgcttacaag tatcggccat atcagccgcg
    20341 ataccggcgc tatactccgc gcgccaggtg ctgatttgct ccgccagcgg gatcagctct
    20401 ttatcccacg ggcgtagctg ttcgtaacgt gtcacctggc gcagcgccgc attgcgctgc
    20461 ttgagcaatc gcttgagatt gctccaggcg gtgaaaaatc cgggttcgtt gtgaaagcat
    20521 ccccagtcga ggaatgctct tctgtatttg gggccgccgt tgagtaaagt aaacccttct
    20581 ggcgttatca actgcattgg catcaggtgc gccagttccg cgaccttatg cccgtctgta
    20641 ccgtcgatgc ggactttgct gtcgccctgt ttgtctttgg ttaagccaat cgctgtctcg
    20701 cgctcttcgc cctgtaatcg cccgtggaga acaaacgcct cctgctcatg gcgaatgacg
    20761 cgaccaatct gcaaactgcg aaacgcccga ccatggccga gcgtatagat ggcttccagc
    20821 acgctggttt tgccactgcc gttggcacct accagaaagt taaagccggg agataaagcg
    20881 agatccgcgg tttcaatgtt gcggaaatcg cggatcaaca agcgggtgag ggacattaca
    20941 gtctcattgg catgacaaca taggcagccg actgtgatgc ggcatcttca atctgcacgc
    21001 tggaaaccga atcggtcagc atcatgcgga cgttttcaca tttcagcgcg ttcagaacat
    21061 ccagcacgta gctgacgttg aagccgattt ccatctccgc accgctatag gtaacgtcga
    21121 ggatctcttc cgcttcttcc tgttccgggt tgttggcggt gattttcagc tggttttcgc
    21181 tgacataaag acgtacgccg cggaatttct cgttagagag aatcgccgcg cgagcaaacg
    21241 cctgcttgag cagatcgcag ccagcttcca gatgtttgtc cgggttcttc ggcagaacgc
    21301 ggcgataatc cgggaagcga ccatccacca gtttggaggt gaagataaag tcgccaacgt
    21361 gggcgcgaat gttgttgctg ccaatctgta cgcgcagcgg attgtcgccg ccgtcgagca
    21421 tacgcatcag ttcaatcacg cctttacgcg gcacgatcac cgaatggctt ggcaaagatt
    21481 gaccaattgg cattgaacag actgccagac ggtggccgtc ggtcgccacg gtgcgcagtt
    21541 cttcgccttc ggtttcaaac agcataccgt ttaagtaata gcgaacgtcc tgatgcgcca
    21601 tagaaaactg ggtcgcttca atcagacgct tcatcgtcgc ctgcggcagg gtaaattcga
    21661 cttcgctctg ccagtcatcg aggttcggga aatccgccgc tggcagggta gacagcgaaa
    21721 aacggctacg cccggagcgc accagcatcc gttcaccttc cagctgtacg gcaatttccg
    21781 cgccttcagg cagaccacgg cagatatcaa agaatttgcg cgccggaacg gtcgtcgctc
    21841 ctggctcgtg tggctgaacc agcgcaacgc gtgccaccat ttccatctcg agatcagtac
    21901 cggtcagcga caacgtaccg tcagcaacct gtaacagcag attaccgaga atcggtagcg
    21961 taggacgacc acctaacggc ccgctcacct gttgtagcgg ttttaataaa tgctcacgtt
    22021 ctacggtaaa tttcataggt ttacgatgac aatgttctga ttaaatttga aaaatcttct
    22081 ttgatatcgt ggctctcttc acgcaactgc tcgatcttac ggcaggcatg aagcaccgtc
    22141 gtgtggtcac gaccaccaaa cgcatcgcca atctccggca gactgtggtt agtcagctct
    22201 ttcgccagcg ccatcgccat ctggcgcgga cgcgccaccg agcgggatcg acgcttggaa
    22261 aggagatccg cgactttgat cttgtagtac tccgccaccg tcttctgaat attgtcgatg
    22321 gtgaccagtt tttcctgcaa tgccagcaag tcgcgcagtg cctcacgcac gaagtcgatg
    22381 gtgatcgccc gtccggtaaa gttggcgtta gcaataacgc ggttcagcgc cccttccagc
    22441 tcacgtacgt tagatcgtag acgcttggcg ataaagaacg ccacttcgcc tggcaaacga
    22501 atgtcatttt cgtcggcctt tttcatcagg atcgccacgc gggtttccag ctctggcggt
    22561 tcgatcgcca cagtcagtcc ccaaccgaag cgggatttca aacgatcctc aacgccgttg
    22621 atctctttcg gatagcgatc cgaggtgaga atgatctgtt gattaccttc cagcagggcg
    22681 ttgaaggtgt ggaaaaactc ttcctgagat cgttctttat tagcaaaaaa ctgaatatcg
    22741 tcgatcagca gtgcatctac ggaacggtag tagcgtttaa actcttcgat cgcgttgttt
    22801 tgcagggctt taaccatgtc ctgaacaaag cgctcggagt gcatataaac cactttggca
    22861 ttcggcttgc gcgccataat gccgttaccc accgcatgca gcaggtgagt tttacccaga
    22921 cccgtgccgc cataaaggaa caatgggtta taggcaccgc cagggttatc cgccacctgg
    22981 cgagccgccg cgcgcgccag ttggttagat ttaccttcaa cgaagttatc aaacgtgtgt
    23041 ttgacgttta cgttagaacg ataggtcggt tctgccgggg ccgggacgtt atcccaacct
    23101 gagcgcgtag aaggcgcagc acgttgcggc tgcgtttgcg ccacctgtgc aggggccgcg
    23161 acgttgctcg tcactgccgc ttgtggcgtt tgcgtcaccg gtttggtgcc gacttcaaaa
    23221 cgcagctgtg gggcatccgc tccgcagaaa ctggttagca gtccattgat attattaagg
    23281 tacttgtccc gtacccaatc gaggacaaaa cggtttggcg cgtacagggc cagcgtgtta
    23341 tcgctcagtt ccgcctgcaa tgggcgtatc cacatactga attctgtggc tggtaactca
    23401 tcctgcaatc gggcaagaca ctgctgccaa agcgaaagtg acacggcgga ctccactcga
    23461 acaaaagtcg ataatgacta aggctgaaac attcatgatt gttgacgtac gtcgaaaaga
    23521 ccctgcttga gggtgacgca cgaaccgctg tctgcggtta tatgcccgat caagatcctg
    23581 caaaacgatc gggaccgcgg atcatagcct aaactgcgca agagatcttc tgtttctcac
    23641 agattttccc gatttatcca caggactttc cagacctcgc taagtgtaaa cgatcctgcc
    23701 gcgaggcggg cacgatttac gccgcatatt ggaaaattta atgaccatag acaaaaattg
    23761 gcttaatcga tctaataaag atctaggacg atccttgcgc tttacccatc agcccgtata
    23821 atcctccacc cggcgcgcca tgctggtttt cactggtgtg aggtcgtaca ttttccctgc
    23881 gaaaaggtgc ggaaaagcgc ggtaaataag gaaagagaat tgactccgga gtgtacaatt
    23941 attacaatcc ggcctcttta atcacccatg gcttcggtgt ccatcgtttc atttttcggc
    24001 ggatatccaa taaagccatt gaatttattc aagtttaggt agaaatcgcc atgaaacgca
    24061 cttttcaacc gtctgtactg aagcgcaacc gttctcacgg cttccgtgct cgtatggcta
    24121 ctaaaaatgg tcgtcaggtt ctggcacgtc gtcgtgctaa aggccgcgct cgtctgaccg
    24181 tttctaagta ataaagctaa cccctgagtg gttaagctcg catttcccag ggagttacgc
    24241 ttgttaactc ccagtcaatt cacattcgtc ttccagcagc cacaacgggc tggcacgccg
    24301 caaattacca ttctcggccg cctgaattcg ctggggcatc cccgtatcgg tcttacagtc
    24361 gccaagaaaa acgttcgacg cgcccatgaa cgcaatcgga ttaaacgtct gacgcgtgaa
    24421 agcttccgtc tgcgccaaca tgaactcccg gctatggatt tcgtggtggt ggcgaaaaaa
    24481 ggggttgccg acctcgataa ccgtgctctc tcggaagcgt tggaaaaatt atggcgccgc
    24541 cactgtcgcc tggctcgcgg gtcctgatag ccctcattcg ggtctatcaa cgcctgatta
    24601 gtccgctact cgggccgcat tgtcgtttca ctccaacctg ttcaagctac ggaattgagg
    24661 cattgcgcag gtttggagtg ataaaaggca gttggttgac ggtgaaacgc gtattaaaat
    24721 gccacccttt acaccctggt ggtgacgatc ccgtcccgcc cggaccattt gataccagag
    24781 aacactaacg atggattcgc aacgcaatct tttagtcatc gctttgctgt tcgtgtcttt
    24841 catgatctgg caagcctggg agcaggataa aaacccgcaa cctcaggccc aacagaccac
    24901 gcagacaacg accaccgcag cgggtagcgc cgccgaccag ggcgtaccgg ccagtggcca
    24961 ggggaaactg atctcggtta agaccgacgt gcttgatctg accatcaaca cccgtggtgg
    25021 tgatgttgag caagctctgc tgcctgctta cccgaaagag ctgaactcta cccagccgtt
    25081 ccagctgctg gaaacttcac cgcagtttat ttatcaggca cagagcggtc tgaccggtcg
    25141 tgatggcccg gataacccgg ctaacggccc gcgtccgctg tataacgttg aaaaagacgc
    25201 ttatgtgctg gctgaaggtc aaaacgaact gcaggtgccg atgacgtata ccgacgcggc
    25261 aggcaacacg tttaccaaaa cgtttgtcct gaaacgtggt gattacgctg tcaacgtcaa
    25321 ctacaacgtg cagaacgctg gcgagaaacc gctggaaatc tccacctttg gtcagttgaa
    25381 gcaatccatc actctgccac cgcatctcga taccggaagc agcaacttcg cactgcacac
    25441 cttccgcggc gcggcgtact ccacgcctga cgagaagtac gagaaataca agttcgatac
    25501 cattgccgat aacgaaaacc tgaacatctc ttcgaaaggt ggttgggtgg caatgctgca
    25561 acagtatttc gcgacggcgt ggatcccgca taacgacggt accaacaact tctataccgc
    25621 taatctgggt aacggcatcg ccgctatcgg ctataaatct cagccggtac tggttcagcc
    25681 tggtcagact ggcgcgatga acagcaccct gtgggttggc ccggaaatcc aggacaaaat
    25741 ggcagctgtt gctccgcacc tggatctgac cgttgattac ggttggttgt ggttcatctc
    25801 tcagccgctg ttcaaactgc tgaaatggat ccatagcttt gtgggtaact ggggcttctc
    25861 cattatcatc atcaccttta tcgttcgtgg catcatgtac ccgctgacca aagcgcagta
    25921 cacctccatg gcgaagatgc gtatgctgca gccgaagatt caggcaatgc gtgagcgtct
    25981 gggcgatgac aaacagcgta tcagccagga aatgatggcg ctgtacaaag ctgagaaggt
    26041 taacccgctg ggcggctgct tcccgctgct gatccagatg ccaatcttcc tggcgttgta
    26101 ctacatgctg atgggttccg ttgaactgcg tcaggcaccg tttgcactgt ggatccacga
    26161 cctgtcggca caggacccgt actacatcct gccgatcctg atgggcgtaa cgatgttctt
    26221 cattcagaag atgtcgccga ccacagtgac cgacccgatg cagcagaaga tcatgacctt
    26281 tatgccggtc atcttcaccg tgttcttcct gtggttcccg tcaggtctgg tgctgtacta
    26341 tatcgtcagc aacctggtaa ccattattca gcagcagctg atttaccgtg gtctggaaaa
    26401 acgtggcctg catagccgcg agaagaaaaa atcctgattc ggtgagtttt cgctaaaata
    26461 agggcggtca gttgaccgcc ttttttcttt tcgtagggtg gataagcacc gcgcatccgc
    26521 cacacaaagc aacaggaaca tcatgagcga taatgacact atcgtagccc aggccacgcc
    26581 tccgggacgt ggcggcgttg gcatcctgcg catctccggc ctaaaagccc gtgaagttgc
    26641 cgaaaccgtg ctgggtaaac tgcctaagcc gcgctacgcc gattatcttc cgtttaaaga
    26701 cgccgacggc agcgtgctcg atcaggggat tgcgctatgg ttccctggcc cgaactcgtt
    26761 caccggcgaa gatgtgctgg aactgcaggg tcatggcggt ccggtgatcc tcgacctgct
    26821 gttaaaacgc attctgacca ttcccggcct gcggattgct cgccctggtg agttttccga
    26881 acgcgcgttt cttaacgata aactcgactt agcccaggcc gaggcgattg ccgatcttat
    26941 cgacgccagt tcggaacagg cggcccgttc ggcacttaac tcgctgcaag gcgcattctc
    27001 cgcacgggtc aaccatctgg tggaagccct cacccacttg cgcatttacg tcgaagcggc
    27061 aattgatttc cctgatgaag agatcgattt cctctccgac ggcaaaattg aagcccagct
    27121 taatgacgtg attgccgatc tcgatgcagt gcgtgctgaa gcacgtcagg gtagtttgtt
    27181 gcgcgaaggg atgaaggtgg tgattgccgg acgtcctaac gccggtaaat cgagcctgtt
    27241 aaacgcgctg gcgggccgtg aagcagcaat cgtaactgat atcgccggaa ccacacgtga
    27301 cgtgctgcgt gagcatatcc acattgacgg aatgccgctg catatcatcg ataccgctgg
    27361 gctacgtgaa gccagtgacg aagtggaacg tattggtatc gagcgcgcgt ggcaggaaat
    27421 tgaacaggcc gaccgcgtgc tgtttatggt cgatggcacc acaacagacg ccgtggatcc
    27481 ggcagagatc tggccggaat ttatcgcccg tctgccagcg aaactaccga tcaccgtggt
    27541 gcgcaataaa gccgatatca ccggcgaaac gctgggaatg agtgaagtga acggtcacgc
    27601 gttaattcgt ctctcggcaa ggacaggtga aggcgtggag gtgctgcgta accatctcaa
    27661 acagagcatg ggctttgaca ccaacatgga aggcggcttc ctggcgcgtc gtcgccacct
    27721 acaggcgctg gaacaggcag cggagcattt gcagcaaggt aaagcgcaac tgttaggtgc
    27781 ctgggcgggt gaactgctgg cggaagagtt gcgcctggca cagcagaact taagcgaaat
    27841 caccggggaa tttacctctg atgatttgct ggggcggatt ttctccagct tctgtattgg
    27901 taagtaaccg cgcttacgaa gccgtattct gactgtcaga tgcggcttcg cttcattgtt
    27961 agcactcctg ttattcctca accctttttt taaacattaa aattcttacg taatttataa
    28021 tctttaaaaa aagcatttaa tattgctccc
//
LOCUS       DAZPEP010000042        27108 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZPEP010000042 DAZPEP010000000
VERSION     DAZPEP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27108
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.042"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..925
                     /locus_tag="ACHUT9_001761"
     CDS             <1..925
                     /locus_tag="ACHUT9_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1348256.1"
                     /translation="TSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL
                     NDSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPV
                     NIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNL
                     TAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQ
                     MRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGT
                     VNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(968..1828)
                     /gene="rsmI"
                     /locus_tag="ACHUT9_001762"
     CDS             complement(968..1828)
                     /gene="rsmI"
                     /locus_tag="ACHUT9_001762"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFR1348257.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1892..3928
                     /gene="lpoA"
                     /locus_tag="ACHUT9_001763"
     CDS             1892..3928
                     /gene="lpoA"
                     /locus_tag="ACHUT9_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFR1348258.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3886..4281
                     /locus_tag="ACHUT9_001764"
     CDS             3886..4281
                     /locus_tag="ACHUT9_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFR1348259.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4301..4891
                     /gene="diaA"
                     /locus_tag="ACHUT9_001765"
     CDS             4301..4891
                     /gene="diaA"
                     /locus_tag="ACHUT9_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFR1348260.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4901..5476
                     /gene="dolP"
                     /locus_tag="ACHUT9_001766"
     CDS             4901..5476
                     /gene="dolP"
                     /locus_tag="ACHUT9_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFR1348261.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5590..6630)
                     /locus_tag="ACHUT9_001767"
     CDS             complement(5590..6630)
                     /locus_tag="ACHUT9_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1348262.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6703..7338)
                     /locus_tag="ACHUT9_001768"
     CDS             complement(6703..7338)
                     /locus_tag="ACHUT9_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFR1348263.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7466..7984
                     /gene="yhbO"
                     /locus_tag="ACHUT9_001769"
     CDS             7466..7984
                     /gene="yhbO"
                     /locus_tag="ACHUT9_001769"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFR1348264.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7964..8407)
                     /locus_tag="ACHUT9_001770"
     CDS             complement(7964..8407)
                     /locus_tag="ACHUT9_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFR1348265.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8458..8760
                     /gene="yhbQ"
                     /locus_tag="ACHUT9_001771"
     CDS             8458..8760
                     /gene="yhbQ"
                     /locus_tag="ACHUT9_001771"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFR1348266.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8747..9250)
                     /locus_tag="ACHUT9_001772"
     CDS             complement(8747..9250)
                     /locus_tag="ACHUT9_001772"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1348267.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9244..9768)
                     /locus_tag="ACHUT9_001773"
     CDS             complement(9244..9768)
                     /locus_tag="ACHUT9_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFR1348268.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9977..10972
                     /locus_tag="ACHUT9_001774"
     CDS             9977..10972
                     /locus_tag="ACHUT9_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFR1348269.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10981..11859
                     /locus_tag="ACHUT9_001775"
     CDS             10981..11859
                     /locus_tag="ACHUT9_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFR1348270.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDKLGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12065..13072
                     /locus_tag="ACHUT9_001776"
     CDS             12065..13072
                     /locus_tag="ACHUT9_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFR1348271.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13190..14434)
                     /gene="mtr"
                     /locus_tag="ACHUT9_001777"
     CDS             complement(13190..14434)
                     /gene="mtr"
                     /locus_tag="ACHUT9_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFR1348272.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14588..16477)
                     /gene="deaD"
                     /locus_tag="ACHUT9_001778"
     CDS             complement(14588..16477)
                     /gene="deaD"
                     /locus_tag="ACHUT9_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFR1348273.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16470..16550)
                     /gene="yrbN"
                     /locus_tag="ACHUT9_001779"
     CDS             complement(16470..16550)
                     /gene="yrbN"
                     /locus_tag="ACHUT9_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFR1348274.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16657..17541)
                     /gene="nlpI"
                     /locus_tag="ACHUT9_001780"
     CDS             complement(16657..17541)
                     /gene="nlpI"
                     /locus_tag="ACHUT9_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFR1348275.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17650..19785)
                     /gene="pnp"
                     /locus_tag="ACHUT9_001781"
     CDS             complement(17650..19785)
                     /gene="pnp"
                     /locus_tag="ACHUT9_001781"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFR1348276.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20032..20301)
                     /gene="rpsO"
                     /locus_tag="ACHUT9_001782"
     CDS             complement(20032..20301)
                     /gene="rpsO"
                     /locus_tag="ACHUT9_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFR1348277.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20450..21394)
                     /gene="truB"
                     /locus_tag="ACHUT9_001783"
     CDS             complement(20450..21394)
                     /gene="truB"
                     /locus_tag="ACHUT9_001783"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFR1348278.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21394..21795)
                     /gene="rbfA"
                     /locus_tag="ACHUT9_001784"
     CDS             complement(21394..21795)
                     /gene="rbfA"
                     /locus_tag="ACHUT9_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFR1348279.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21959..24631)
                     /gene="infB"
                     /locus_tag="ACHUT9_001785"
     CDS             complement(21959..24631)
                     /gene="infB"
                     /locus_tag="ACHUT9_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFR1348280.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24656..26143)
                     /gene="nusA"
                     /locus_tag="ACHUT9_001786"
     CDS             complement(24656..26143)
                     /gene="nusA"
                     /locus_tag="ACHUT9_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFR1348281.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26171..26623)
                     /gene="rimP"
                     /locus_tag="ACHUT9_001787"
     CDS             complement(26171..26623)
                     /gene="rimP"
                     /locus_tag="ACHUT9_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFR1348282.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26830..26906)
                     /locus_tag="ACHUT9_001788"
     tRNA            complement(26830..26906)
                     /locus_tag="ACHUT9_001788"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26870..26872),aa:Met,seq:cat)
BASE COUNT         6423 a         7517 c         6809 g         6359 t
ORIGIN      
        1 tacctctgca acgctaagta gcactaactg gcaatacgcc tgttcctgct ctgcgggtaa
       61 ggcagttaaa cttgtctata tggtcagccc cgtacttacc accactggac atcagacagg
      121 atattacaaa ctcaatgaca gcctggatat taaaaccaca ttacaggcaa acgacattcc
      181 aggactcaca accgaccagg ttgtctctgt taacacccga ttcacacaga taaaaagcag
      241 caccgtatat tctgctgcaa cccaaacggg tgtttgccag ggtgacacgt ctcgttatgg
      301 acccgttaat attggtgcaa ataccacctt taccctgtat gtcaccaagc catttctcgg
      361 ctcgatgacc attccgaaaa cggatattgc cgtcattaaa ggcgcgtggg tcgatggaat
      421 gggaagcccg tctacaggtg acttccatga tttagtcaag ttatcgattc agggaaatct
      481 caccgcccca cagtcgtgca aaattaatca gggcgatgtt attaaggtta attttggatt
      541 catcaatggt cagaagttta ccacccgcaa tgccatgcca gacggtttta ctccagtaga
      601 ctttgatatc acttatgact gtggtgatac ttcaaagatt aaaaactcgt tgcaaatgcg
      661 catcgacggt acaactgggg tagtagacca gtacaacctg gtcgccaggc gaagaagttc
      721 agacaatgtg cccgatgtcg gtattcgtat tgaaaatctc ggcggcggag ttgcaaatat
      781 tccttttcag aacggtatcc ttcccgttga tccttccggg catggcaccg tcaatatgcg
      841 cgcctggcca gttaatctgg tcggtggtga gctggaaaca ggaaaatttc agggcaccgc
      901 caccattacc gtcatggtgc ggtaaaagaa gacgttgcgt tttgattaac gcaacgcttc
      961 ggcactgtta cccctgctgc tccagcgcat acttatacag cgcatttttc ttcacgccat
     1021 gaatttctgc ggccagcgcc gccgcttttt tcagcggcag ttctgcctgt agcagcgcca
     1081 gcgtgcgcag ggcatcggcg ggtaagtctt cttcctgtgc tttatgacct tcgacaatca
     1141 gcaccatttc gcctttgcga cggttttcat cttcctttac ccacgccagc agctcgccaa
     1201 cgggcgcgcc gtgaatggtt tcccaggttt tggtcagctc acgcgccaga accacgtagc
     1261 gggattcgcc taataccgca acgatatctt ccaggctatc taacagacgg tgggtagatt
     1321 cataaaaaat cagcgtgcgc ggctccgctt caatggcttt tagcgcatca cggcggcctt
     1381 ttgatttggc aggtaaaaag ccttcgtaac agaaacggtc agagggtaaa cccgcggcgc
     1441 ttaacgcagt gatagcagca cacggtccgg gtagcggcac cacacgaatc cccgcttcac
     1501 ggcaggtacg caccagatgg tagccaggat cgttaattag cggcgttccg gcatcggaaa
     1561 ccagcgcaat gttttgcccc tcttgcagct tcgccagcag cgtttcggct ttttgttgtt
     1621 cgttatggtc gtgcagcgca aacaaccggg cattaatccc aaaatgttgc agcaataaac
     1681 cggtgtgacg agtatcctcg gcggcaatca gatcaacggc ctgtaatacc tctaacgcac
     1741 gctgggtgat atccgccaga ttgccgattg gcgtcggtac aatgtaaagc tggccctgag
     1801 aattatccgc cgattggtgt tgtttcattg tgtcgtccgt attgccgatt taatattgag
     1861 cattgcgtaa aaaaatatca ctggatacat tatggtaccc tcaacatttt ctcgtttgaa
     1921 agccgcgcgt tgtctgcctg ttgttctggc agccctgatt ttcgccggtt gtggcaccca
     1981 tactcccgat cagtccactg cttatatgca gggcacggcg caggctgatt ctgcctttta
     2041 tcttcagcag atgcagcaaa gctctgatga taccaggatc aactggcaat tactcgccat
     2101 tcgtgcactg gtgaaagaag gtaaaaccgg gcaggcggtt gagttgttta accaactacc
     2161 gcaagaactg aacgattctc agcgtcgcga gaaaacactg ctggcggtag agattaaact
     2221 ggcgcagaaa gattttgctg gcgcgcaaaa cttgctggcg aaaatcacac ctgccgattt
     2281 agaacaaaac cagcaagcgc gttactggca ggcaaaaatc gatgccagcc aggggcgtcc
     2341 ttccattgat ttactgcgcg cgttaattgc tcaggaaccg ctgctcggcg cgaaagaaaa
     2401 aaagcagaat attgatgcca cctggcaggc gctctcctcc atgactcagg aacaggcgaa
     2461 tacgctggtg atcaacgccg acgaaaatat tctgcaaggc tggctggatc tgcagcgcgt
     2521 ctggtttgat aaccgtaacg atcccgacat gatgaaagcc gggatcgccg actggcaaaa
     2581 acgttatccg aacaatccgg gcgcgaaaat gctgccaacg cagttggtta acgtaaaagc
     2641 gtttaaacca gcctcgacca acaaaatcgc cctgctgttg ccgctgaatg gccaggcagc
     2701 ggtatttggt cgcaccattc agcaaggctt tgaagcggcg aaaaatatcg gcactcagcc
     2761 agtggcggct caggtagctg ccgcacctgc cgcagacgta gctgaacaac ctcagccgca
     2821 aaccgtggat ggcgttgcca gcccggcaca agcctcggtt agcgatctga ccggtgatca
     2881 gcctgcggcc cagccggtgc ctgtaagcgc cccggcgaca agcaccgcag cggtaagcgc
     2941 acccgcaaat ccatccgcag agctgaaaat ctacgatacc tcatcacaac cacttagcca
     3001 gatcttaagc caggttcagc aggatggcgc gagtattgtg gttggtccgt tgctgaaaaa
     3061 taacgttgaa gagttgctga agagcaacac cccgctgaac gtgctggcac tgaaccagcc
     3121 agagaatatc gaaaaccgcg tcaatatttg ttacttcgcg ctttcacctg aagacgaagc
     3181 gcgcgatgca gcgcgtcata ttcgtgacca gggtaaacaa gcgccgctgg tgctgatccc
     3241 acgcagttca ttgggcgatc gcgtagccaa tgcgtttgcg caagagtggc agaaactggg
     3301 cggcggcacc gttctgcaac aaaaatttgg ttccaccagc gaattacgcg cgggtgttaa
     3361 cggcggttct ggtatcgctt taacgggtac cccgattact cccagagcga caaccgactc
     3421 cggcatgacg accaacaatc caacgctgca aaccacgcca accgatgacc agttcaccaa
     3481 taatggcggt cgtgtcgatg cggtgtacat tgtggcaacg ccgggtgaaa tcgcttttat
     3541 caaaccgatg atcgccatgc gtaacggtag ccagagcggt gcaatgctgt acgccagctc
     3601 ccgcagcgca caaggcaccg caggcccgga tttccgtctg gagatggaag gtttgcagta
     3661 cagcgaaatc ccgatgctgg cgggcggtaa tctgccgtta atgcagcagg cactcagcgc
     3721 ggtgaataac gattattcac tggctcgcat gtatgcgatg ggcgtcgatg cctggtcgct
     3781 ggcaaatcat ttctcacaaa tgcgccaggt tcagggtttt gaaatcaacg gtaataccgg
     3841 aagcctgacg gcaaacccgg attgcgtgat taacaggaag ttatcatggc tacagtacca
     3901 acaaggtcag gtagtccccg tcagttaacc accaaacaga ccggcgatgc gtgggaagca
     3961 caagcgcgtc gctggctgga aggcaaagga ctgcggttta tcgccgctaa cgtgaacgag
     4021 cgtggcggcg agatcgatct gataatgcgt gaaggccgga ccaccgtttt tgtcgaggta
     4081 cgctatcgcc gctctgcgct ttatggcggc gcggcagcca gtgtgacccg cagcaaacaa
     4141 cacaaattat tacagactgc ccgcttgtgg ctcgcgcgtc ataatgggag ttttgatact
     4201 gtggattgcc ggttcgatgt ggtagccttc accgggaatg aggttgagtg gattaaggat
     4261 gcctttaatg accactcata attaaggttt aaggattagc gtgcaagaaa gaattaaagc
     4321 ttgcttcact gaaagcattc aaactcaaat tgcggcggca gaggcgcttc cggatgccat
     4381 ctcccgtgca gccatgacgc tggttcagtc tctgctcaat ggcaacaaaa tcctctgttg
     4441 tggtaatgga acttccgctg ccaatgcaca gcattttgct gccagcatga tcaaccgttt
     4501 cgaaacggag cggcccagct tacctgccat tgcactaaat actgataatg ttgtcttaac
     4561 ggcgattgcc aacgatcgct tacatgatga agtgtatgca aaacaggtgc gggcgctggg
     4621 tcatgcggga gatgtattgt tagccatttc cacccgtggc aacagccgcg atattgttaa
     4681 agcagttgaa gccgccgtta cgcgtgatat gaccattgtg gcattgaccg gctatgacgg
     4741 cggcgagctt gcaggtttgt tagggccaca ggatgtggag atccgcattc cttcgcatcg
     4801 tagtgctcgc attcaggaaa tgcatatgct gacggtaaat tgcctgtgcg atctgatcga
     4861 taacacgctt ttccctcacc aggatgatta aggagaatac atgaaggcat tatcgccaat
     4921 cgcagtcctt atttccgcgc tgctgttgca aggttgtgtt gccgctgccg tagtgggtac
     4981 cgctgctgtg ggtaccaaag ccgcaactga cccacgcagt gtcggcaccc aggtggacga
     5041 tggtaccctg gaagtgcgcg tgaacagcgc attgtcgaaa gacgaacaga ttaagaaaga
     5101 aacccgtatt aatgtcacgg cgtatcaggg caaagtgctg ctggttgggc agtcaccaaa
     5161 cgctgaactt tcggctcgcg ccaaacagat tgctatgggc gtagacggtg ccaacgaagt
     5221 gtataacgag attcgtcagg gccagccgat tggtctgggc gaagcatcta acgatacgtg
     5281 gatcaccacc aaagtgcgtt cgcagctctt aaccagcgac ctggtgaaat cgtccaacgt
     5341 gaaagtgacc accgaaaacg gtgaagtgtt cctgatgggg ctggtgactg aacgtgaagc
     5401 gaaagcggcg gcagatattg ccagccgggt gagcggcgta aagcgggtaa ccacagcgtt
     5461 tacgtttatt aaatagcagc cccttgtaat gcctgatgcg acgcttgccg cgtcttatca
     5521 ggcctacaac gatgcaaacc gtaggtcgga taaggcgttt acgccgcatc cgacatgttt
     5581 tccctcaaat cagaataaca gcgccagccc gccgacaatc acgcctgaca ctgccaccat
     5641 cgcccccgtc agccataagg ctttcgccgg gaacgctttg cgcagcataa tcagtgacgg
     5701 caaactcact gccgggagcg tcatcaacaa tgccagcgcc ggagcggttc ccatacctgc
     5761 cagcatcatc gtttgtacaa tcggaatttc tgctgccgtg ggaatgacaa acaagcatcc
     5821 tgctaccgcc atcgccacca cccacatcag gctgttatcg acagcaccat cggcatgggg
     5881 gaataaccag acgcgtgcgg cacccaacac cagtactgca aggatgtaaa ccgggatcgt
     5941 actccagaaa agtgtccata gcgccctgcc ccagcggcta aaaaaaccgc tctgtgcttc
     6001 cggtatgtca atttcgaccg gtgcctgcgt ttgcggtgtt tcacgcaccc atttttgcac
     6061 cagcgtcgca atcagcaaca ccatcaccag cccggccacc agacgaatcg ccgcaaaacc
     6121 ccagccgagg acaaacccca taaacaccag cgtcgccggg tttaacaccg gattgcccat
     6181 ccagaatgcc agcgcaccgc ccatcgatac ctgttggcga cgcattcccg ccgcgaccgg
     6241 agccgcacag caggtacaca tcatgcccgg cagcgaaaac agcgttccca gcagcgtgcc
     6301 gcgaaagcgc gattgtccaa gcgtacgcaa caaccagtca cgcgggatca gcacctgaat
     6361 caacgaaccg aggatcaccc ccagcaccgc cgctttccat accgcgagga aatagatcat
     6421 cgcgtaatcc aacgccgcct gccacggatt agcatccgcc tgcgcaagga tagatttacc
     6481 gatactgtgg gtttcggcag cagtaaacgc tttgccgtag taaggttgcc atttcacata
     6541 ccagagaccg gcaatgacaa cgagaaagaa aagcgcgggt ttccaccact gaatgggcgt
     6601 tgccgcctga gatgaagact gaccagtcat agcattcccc aggaataagt tatgataatt
     6661 gagcgcgtga atattacgct cactatcaat tcttgggaat aattattcag ctcttttgcg
     6721 taattctgaa gagcttaaaa tagtcacgcc ttcatgctca ggccgcatcg cctctgccag
     6781 catgacccgc gccacgtctc tcgcatcaat ggatttccag ttaccgggca gtaaacgaaa
     6841 cagcggcgca aaaagcgttt cgttcatccg ctgtttgcta cgatcgccca gcaacatcga
     6901 cgggcgagca atggtcaatt tcggccagtt ctgggcaatt aatgcttctt ccatctcccc
     6961 tttgacgcgg ttatagaaaa acggcgagtg ggcattggca cccatagcac tgaccaccag
     7021 catatgctgc gcgcccagtc gccgcccggt taatgcggta tccactacca gcgtgtaatc
     7081 ggcatgaata aacgcctctt tgctccccgc ttctcgccgc gtggtgccga gacaacaaaa
     7141 cacaatgtcg atgggttcgg tgacctgcgc caacgcgtca ctcagttgcg gatcatgggg
     7201 attaaacacc ccgggcatat cgcccaacgg acgtcgcgtc ggcgcggcta tggcgttaac
     7261 tttcggttcg ttaatcaaca tccgcagcag gtgaccgccc accagccccg ttgcgcctgt
     7321 aatcagtacc tgactcatct tcgctccttt acagaattgt ctgccttgcg ctccacggct
     7381 catgcaccag gcttaataga ccatgaggta attatccccg attgtgtgaa attcgcctca
     7441 tccaatgcaa caacgcggag gaagcatgag taagaaaatt gccgttttaa tcagtgatga
     7501 atttgaggat tcagaattta cttcacccgc agacgagttc cgtaaagccg gacacgaagt
     7561 gattaccatt gaaaaacaag cgggtaaaac ggtgaaaggc aaaaaaggag aagccagcgt
     7621 gaccatcgat aaatccatcg atgaagtgac gccagcggag tttgatgccc tgctgctacc
     7681 gggtggtcat tcaccggatt atctgcgtgg cgacaaccgt tttgtcacct ttacccgtga
     7741 ttttgtgaat agcggcaaac ctgttttcgc catctgtcac ggtccgcagc tgctgatcag
     7801 cgccgatgtg attcgggggc gcaaactgac cgcagttaaa ccgatcatta ttgatgttaa
     7861 aaatgcgggc gcggaatttt acgatcagga agtcgtggtt gataaagatc agctggttac
     7921 cagccggaca ccggacgatc tgccagcgtt taaccgcgag gcgttacgcc tgctcggtgc
     7981 ctgagtcgcg tagccaaatc atttttttac caaagcccag cgtgttgtca gtgaatttga
     8041 tttcatcgag ccggatttcc cacaccgggg ccgacagcat tctggcaacc ggaaagcgac
     8101 gattgtacgc ctggcgcgcg aggtcgcttt cctcaccttc cagcctgcgg atctcacctt
     8161 taaactgcac gccgcgaatt aacgctaccg ttttcggctg accgtttacc gttccggcaa
     8221 ccgcagcctg cggcccactc atctgcgcgt ggcgcgtttt ttcttccgtc aaaatgtaga
     8281 aggcgacttt ctgcgcatca aacagataaa aagcattagc gcaccacagt tccccttcct
     8341 gctgcacaca ccaggtgaca acatgttgtt ttgccaacca acggctgatg gtgatgagtg
     8401 tttccattgc tgctctcttt tatactgtgg gcctgacctt aacatgcgac gcagacgatg
     8461 acaccctggt ttctttactt gatccgtacc gccgacaata agctttatac cgggatcacc
     8521 acggatgtcg aacgccgcta tcagcagcac caaagcggca aaggggcgaa agcactgcgc
     8581 gggaaaggag aactgacgct ggcgttttct gcgccagtgg gcgatcgttc gctggcgtta
     8641 cgggcggaat atcgcgttaa gcaactgacc aaacggcaaa aagagcgtct ggtagcggaa
     8701 ggcgcagggt ttgcagagct gttaagcagt ctgcaaaccc cggagattaa aagcgattga
     8761 aatgctcgtg atactcaacc agaccggtta cgccattcag cgcgtcatcc gctaaacgat
     8821 gtacctggaa ggcgctttca gtgcccggcc agcggcaacg cagatcgtga tgcgccgcca
     8881 gttcaaagcc gaaacgactg tacagcgccg gatcgcccag cgtcaccact gcggcatagc
     8941 cgaactcatt aagcgaatcg agtccttcat agaccagttg gcgtgccagc ccctgtccac
     9001 ggtatttttc atcaaccgcc aacggtgcca tgccgaccca ttgcaggtct tcgccctgca
     9061 catcaaccgg actaaatgcc acatagccaa tgacctgacc ttcgtcatct gtcgccacca
     9121 gccccagcgt cagaaagcca tcttcacgca gatcgtgaac cagcttcgct tccgcatcac
     9181 tttcgaatga gcgacgcagc agggcatcaa tacccggcgc atcaatggga atttctactc
     9241 gaattagcat ggttcaccta ccgatgtctg tttggtttca ggcgcggttt tcattcccgc
     9301 ctcaacaaaa tccgccagtt gcagcagcat catgcgtaag gctttcggca tttgctccag
     9361 ttcaatggcg tccatcaggt ttttcacata cagccccagc tccgtatcgc cttcaatcac
     9421 cagccgacgc tggaagaaga gcgtatccgg atcttgttta cgcgccgcaa tcatcagcag
     9481 atcgctggcg tcggcactaa aactcacatc agcttgcgcg ttctggctga ccaccagttt
     9541 gccattcacc accgaggtaa accattgcag gtcaatatca cgcacatgaa tacttaacca
     9601 gcggccttca agaaactcca gctcaccatc atccagcgcc tgacggaatt gccagcttaa
     9661 gacctgctca agaacctggc gttttagcgc aaatggcgtc agttttaccg gtacactcaa
     9721 cagagatggc cccaaatgca caatacggga acgcagttta tccaacacga gttttactcc
     9781 ctgtttcaac aatcatccta ttttgccata tcagaaaaat aacatagcgg tataaatcaa
     9841 caattccata tgaaattgct gctaccacca atacaacttt aactgcctta aatcaaaaat
     9901 tgtcgcagca aggttaacta aaatcccagt tcgttaacat ttttgcgttt tgaaagcgca
     9961 accttcagga aaaattatgg agctgctctg ccctgccgga aatctcccgg cgcttaaggc
    10021 ggccatcgaa aacggcgcag atgctgttta tatcgggcta aaagatgata ctaatgcccg
    10081 tcacttcgcc ggccttaact ttaccgagaa aaaattgcag gaagcggtga gttttgtcca
    10141 tcaacatcgc cgcaaacttc acatcgcgat taacactttt gcgcatccgg acggttacgc
    10201 ccgttggcag cgcgccgtgg atatggcggc gcagctgggt gccgacgcgc tgatcctcgc
    10261 cgacctcgcc atgctggagt acgccgccga gcgttacccg catattgagc gtcatgtgtc
    10321 agtgcaggct tcggcgacca atgaagaggc gattaacttt tatcatcgcc attttgatgt
    10381 tgcccgcgtg gtgctgccgc gcgtgttgtc gattcatcag gtgaaacagc tggcacgggt
    10441 cacacctgtc ccgctggaag tctttgcttt tggcagcctg tgcattatgt cggaaggtcg
    10501 ttgctatctg tcgtcgtatc tgacgggtga gtcgcccaac accgtgggcg cgtgttctcc
    10561 ggcccgtttc gtgcgctggc aacaaacgcc gcaggggctg gaatcccgcc tgaacgaagt
    10621 gctgatcgac cgttatcagg atggcgaaaa cgcaggttat ccgacgctgt gtaaagggcg
    10681 ttatctggtg gacggcgagc gctatcacgc gctggaagaa ccaaccagtc tcaataccct
    10741 ggaactgctg ccggagttaa tggcggcgaa tattgcttcg gtgaaaattg aaggccgcca
    10801 gcgtagcccg gcgtatgtca gccaggtggc gaaagtctgg cgtcaggcta tcgaccgttg
    10861 taaggccgat ccgcaaaact tcgtaccgca aagcgcgtgg atggagacgc tcgggtcgat
    10921 gtccgaaggc acgcagacca cccttggcgc gtatcaccgt aaatggcagt gagaagagca
    10981 atgaaatatt ccttagggcc agtgctgtgg tactggccaa aagaggcgct ggaagaattt
    11041 tatcagcagg ccgccaccag cagcgccgac gtgatttatc ttggtgaagc ggtatgcagc
    11101 aagcgccggg caaccaaagt tggcgactgg ctggagatgg caaaatcgct cgccggaagc
    11161 gggaagcaga tagtgctctc cacgctggcg ctggtgcagg catcctctga actgggcgaa
    11221 ctgaaacgct atgttgagaa cggtgagttt ctgattgaag ccagcgatct cggcgtggtg
    11281 aatatgtgcg ctgaacgcaa actgccgttc gtcgccgggc acgcgctgaa ctgctacaac
    11341 gcggtgacgc tgaaaatatt gctcaaacag ggcatgatgc gctggtgtat gccggtggag
    11401 ctttcccgcg actggctggt gaatctgctt aatcagtgcg ataagttggg gattcgcaac
    11461 cagtttgaag tggaagtcct gagctacggt catctaccgc tggcctactc cgcccgctgc
    11521 tttaccgcgc gttcggaaga ccgcccgaaa gatgagtgtg aaacctgctg cattaagtat
    11581 ccgaacgggc gcaacgtgct gtcgcaggaa aaccaacaag tgtttgtact caatggcatt
    11641 cagaccatga gcggctacgt ttacaacctc ggtaacgagc tgacatccat gcagggattg
    11701 gttgatgtgg tccgcctgtc accgcagggt actgacactt tcgcgatgct cgacgccttc
    11761 cgcgctaatg aaaatggcgc tgcgccactg ccgttgacgg caaacagcga ctgtaacggc
    11821 tactggcggc ggctggctgg gctggagctg caagcctaag taaatatgct cgtcatactt
    11881 caagttgcat gtgctgcatc tgcattcgtt caccccagtc acttacttat gtaagctcct
    11941 ggggattcac tctcttgtcg ccttcctgca actcgaatta tttagagcac agctcacttt
    12001 gttaacaact ttaactactc tttaatgcag tattaaagat taatcggtaa caaagtgagc
    12061 tgttatgact gataaaacca ttgcgttttc gctactcgat ctggccccca ttcccgaagg
    12121 ttcttcagcg cgagaagcat tctcccactc tctcgatctc gcccgtctgg ctgaaaagcg
    12181 cggctatcat cgctactggc tggcagaaca ccacaatatg actggcattg ccagtgctgc
    12241 cacgtcggta ttgattggct atctggcggc gaataccacc acgctgcatc tggggtctgg
    12301 cggcgtgatg ttgcctaacc actcaccgtt ggtcattgcc gaacagttcg gcacgctcaa
    12361 tacactctat ccggggcgaa tcgatttggg gctgggtcgt gcgccaggta gtgaccaacg
    12421 gacaatgatg gcgctgcgtc gccatatgag cggcgatatt gataatttcc cccgcgatgt
    12481 cgcggagctg gtggactggt ttgacgcccg cgatcccaat ccgcatgtgc gcccggtacc
    12541 aggctatggc gagaaaatcc ccgtgtggtt gttaggctcc agcctttaca gcgcgcaact
    12601 ggcggcgcag cttggtctgc cgttcgcgtt tgcctcacac ttcgcgccgg atatgttgtt
    12661 ccaggcgctg catctttatc gcagcaactt caaaccgtcg gcacgactgg aaaaaccata
    12721 cgcgatggtg tgcatcaata ttatcgccgc cgacagcaac cgcgacgctg aatttctatt
    12781 tacctcaatg cagcaagcct ttgtgaagct gcgccgtggc gaaaccggac aactgccgcc
    12841 gccgattcaa aatatggatc agttctggtc accgtctgag cagtatggcg tgcagcaggc
    12901 gctgagtatg tcgttggtgg gtgataaagc gaaagtgcgt cacggcttgc agtcgatcct
    12961 gcgcgaaacc gacgccgatg aaattatggt taacgggcag attttcgacc accaggcgcg
    13021 cctgcattcg tttgaactgg cgatggatgt gaaggaagag ttgttgggat agtgtgtctt
    13081 aacgcgggaa gccttatccg agctggcaac gctgtcctac gtagacctga taagcgaagc
    13141 gcatcaggca ttgtgtaggc agcagaaatg tcggataagg caccgctgat tactgataca
    13201 ccggcagcaa attaaagctc gataagatat ggaccagtgc gttgccaacg ccaaacacca
    13261 gaatcagcgt aatcatcggc ttgccgcccc agacacggaa tttcgggctg ccaaagcgtt
    13321 tacgcgatgc gcgggctaac agcgccggaa caattgccgc ccagatggta gctgctaaac
    13381 cagcataacc aatggcgtac aggaatccgt tcgggaacag tagaccaccc acaacaggcg
    13441 gggcaaaggt cagcaatgcc gttttcaagc gccccagggc tgagtcatcg aaaccaaaca
    13501 gatctgccag atagtcaaac aaacccagcg ttacgccaag gaacgaactc gctaccgcaa
    13561 agtttgagaa cacgaccagc agaaggtcca ggctacggct gttcagcacg ccgcttaatg
    13621 cctgtaccag cacatcaata ttaccgccct tctctgcaat accgataaac tccggacgcg
    13681 ggatgttacc catcgtcgcc agcaaccaga tggtatacag cgccagtgcc atcagcgtac
    13741 cgtacaccag gcatttcacg atggttttcg gatctttgcc gtaatacttc atcaggctcg
    13801 gcacgttacc gtgataacca aacgatgcca gacagaacgg cagggtcatc agcaaatacg
    13861 gcgcataaga tgcattgctt tcggcgacgt tgaacaatgt cgcaggctgc acatgcccca
    13921 gtaggctacc gaaggtgagg aagaaggtaa tgactttcgc ccccagtaca atcgccgtca
    13981 tgcggctgac ggccttagta ctcaaccaca ccacaaacgc taccagcagt gcaaaaccaa
    14041 aacccgccgc ccgtgccggg acgtttagcg acatctctgc gaaggtgtga tgcagaattg
    14101 aaccactggc agaaatatag gcataggtca ggatatagag cacaaaggca atggaaatac
    14161 cgttgaccac gttccagcct ttgcccagca aatctttggt gatggtgtca aaactcgaac
    14221 cgattcggta attcaggtta gcttccagaa tcatcaagcc ggaatgcagc atacagaacc
    14281 aggtaaagat cagcgccgcc attgaccaga aaaaccacgc cccggacatg accactggca
    14341 gagaaaacat ccctgcgcca ataatggtgc cgccgataat caccacgccg ccaagcagcg
    14401 acggtgacgt ttgggtggtg gttagtgttg ccatgagagc ttctctccag tgaaaaatag
    14461 tgcgactgcg ttgttatgca ttgcactgta ccagtacacg agtacaaaag acagaaaaaa
    14521 agccccgatg gtaaaaatcg gggctgtata tattatttta cagattgtgt tcgctgttca
    14581 gcgatgatta cgcatcacca ccgaaacgac gacgaccggt agagtcatca cgacgcggag
    14641 cgcggccttc acgacgttcg ccgctaaaac gacgaccatc accacggcca ccttcacggc
    14701 gttcaccgct gaagttacga ccgccttcac gacgttcgcc accgaaacca cgaccaccgc
    14761 cacgacgctc accgccagta tgcggctgtg catcgcccag taactgcatg ttcatcggct
    14821 tgttgagaat gcgagtgcgc gtaaagtgtt gcagcacttc acccggcata cctttcggca
    14881 gttcgatggt ggagtgagaa gcaaacagct tgatgttacc aatgtaacgg ctactgatgt
    14941 cgccttcgtt agcaatcgca ccaacgatat gacgaacttc aacaccatca tcgcggccca
    15001 cttcaatgcg gtacagctgc atatcgccaa catcacgacg ttcacgacgc ggacgatctt
    15061 cacggtcacc acgcgggcca cggtcgttac gatcgcgcgg accacggtca tcacggtcac
    15121 ggaattcacg tttcggacgc atcggcgcat ctggcggtac gatcagagta cgttcaccct
    15181 gtgccatttt cagcagtgcc gcagccagag tttcgagatc cagctcttca ccttctgcag
    15241 tcggctgaat tttgctcagc agtgcgcggt attgatccag atcgctgctt tccagctgct
    15301 gctgtacttt agcggcgaat ttttccagac ggcgtttgcc cagcagttct gcgttcggca
    15361 gttctacttc cggaatagtc agcttcatag tacgttcaat gttgcgcagc agacgacgct
    15421 cgcggttctc aacgaacagc agcgcgcggc cagcacgacc cgcacgaccg gtacgaccga
    15481 tacggtgaac gtaagactca gaatccatcg ggatatcgta gttaactacc aggctgatac
    15541 gctcaacgtc caggccacgg gctgcaacgt cggtcgcaat caggatgtcc agacgaccat
    15601 ctttcaggcg ttccagtgtc tgttcacgca gcgcctggtt catgtcaccg ttcagcgcgg
    15661 cgctgttgta gccgttacgc tcaagagctt cagccacttc cagagtcgcg tttttggtac
    15721 gaacgaagat aatcgccgca tcaaaatctt ccgcttccag gaaacgtacc agtgcttcgt
    15781 ttttgcgcat accccagaca gtccagtagc tctggctgat gtcaggacgg gtagtcacgc
    15841 tggactgaat gcgcacttcc tgcggctctt tcataaagcg gcgggtaatg cgacgaatcg
    15901 cttccggcat ggttgcagag aacagagcgg tctgatgacc ttccgggatc tgcgccataa
    15961 tggtttcaac gtcttcgatg aagcccatgc gcagcatttc gtcagcttca tccagaacca
    16021 gaccgctcag tttagagagg tccagagtgc cacgtttcag gtggtccagc agacggcccg
    16081 gagtaccgac aacgatctgc ggcccctgac gcagggcgcg taattgcacg tcataacgct
    16141 ggccgccgta cagagcaacc acatttacgc cgcgcatgtg tttagagaaa tccgtcattg
    16201 cttcagcaac ctgtaccgcc agttcgcggg tcggtgccag caccagaatc tgtggtgctt
    16261 tcagctcagg atcaagattc tgcaacagag gtaaagagaa tgctgcagtt tttccgctcc
    16321 ccgtctgggc catacccaga acgtcgcggc cattcagcag atgtggaata cactctgcct
    16381 gaattggaga tggtttttcg taacccagat cgttaagggc ttcaaggata ggagccttca
    16441 ggcccagatc tgcaaaagtg gtttcgaatt cagccatgta gtacgtgtgc ctcaaaatta
    16501 atggcggcca gtctacataa ctcatcatga aattgatcag caattttcat tgaaaagtgt
    16561 gaaccggctc aaagtaggtg tattaacgaa caacaacgcc ctcacccgtt aaggtgatgg
    16621 caatcaaaaa agattacggg ctgatgtgta cgtcagctat tgctggtccg attctgccag
    16681 gtcatcttgg tcctggccca ggagcgataa ttccaacaat gcgtatcggt gctcaacaaa
    16741 gttatgaacg ttgttggcaa ccgccagttt gaacagtgcc gtggcgctgt ccaaatcccc
    16801 cagacttagg tagtacttac ctaaatagaa gttggtttca ctgagatgct cagcgagcga
    16861 ggtgttatcc gttgcgtccg ccttgagcct ttccattaac gtttgttcgc taatgttgcc
    16921 caggtagaac tcgacaatgt tccatcccca ctgttcctta tccgattttt cgaagtgctg
    16981 tttcaacact tctttagcct gcttctcatc gagcttctgc tcggcgagat aaagccacag
    17041 actacggaaa ggatcattgg gatcgtcttg ataaaacgcc agcagatcat cttgcgctaa
    17101 cttgtcacga ccgccgtaat ataatgcgat cccgcgattc aagtgcgcgt agttgtaagt
    17161 tggatcaagc tcaagtacag aatcaaacgc ttcataggca gcatcaaaat tgcctgcctg
    17221 cgttaaatat atgcctaagt aattgaatac ttcaggcata tccggtcgga ttgccagcgc
    17281 ttgcgaaaaa tcgttacgcg ctaatgccct cagaccgaga ctatcataca acactccgcg
    17341 ctcatataaa agctgtgcgc gttcgtcatc ggttaaagcc cgactggcaa ggatttgttc
    17401 catacgtgcc agaatcactt cctgctgtaa agtcggttgc aatggtaccg cgaggacttc
    17461 acttttacgc caggaagtat tactgcatcc tgcaagcgta agtgctgtcg caacgaaaca
    17521 ccagcgcaaa aaaggcttca tttcccactc ccgaagacca cggttgaatg aacgtcctgt
    17581 tcccggttgc taacaaggcg tcctgcccgg ttaaaagccc cccgccgcag cggagggcaa
    17641 atggcaacct tactcgccct gttcagcagc cggagcttcc ggtgctgcag caggttgaga
    17701 ctgctcagtc gcttctttaa tgctcagacg gatacggccc tggcgatcaa cttctagaac
    17761 tttcaccggt acttcctgac ccatctgcag gtaatcggtc actttctcaa cgcgtttgtc
    17821 agcgatttga gagatgtgga ccagaccttc tttaccgccg ccgatggcaa caaatgcgcc
    17881 aaagtcaacg atacgggtca ctttaccagt gtagacgcgg cccacttcga tttctgcagt
    17941 gatctcttcg atacgacgaa tagcatgttt cgctttctcg ccgtcggtcg ctgcgatctt
    18001 cacagtaccg tcatcttcga tttcgatggt agtgccagtt tcttcggtca gggcacggat
    18061 aacagagccg cctttaccga taacgtcttt gatcttatcc gggttgatct tgatggtatg
    18121 gatacgcggt gcgaactcag agatatcgcc acgcggcgcg ttgatcgcct gttccattac
    18181 gcccaggata tgcagacgcg cacctttagc ctggttcagc gcaacctgca tgatctcttt
    18241 ggtgatacct tcaattttga tatccatctg cagtgcagag ataccgtcgc gggaacctgc
    18301 aactttgaag tccatatcgc ccaggtgatc ttcgtcgccc aaaatgtcag acagtacaac
    18361 gtagttgtcg ccttctttca ccagacccat tgcgataccc gcaacggcag ctttgattgg
    18421 cacacctgcg tccatcagcg ccagagacgc gccgcacacg gaagccatag aagaagaacc
    18481 gttggattcg gtgatttcag acactacacg tacggtgtac gggaatttgt ccatatccgg
    18541 catgactgcc agcacgccgc gcttcgccag acgaccgtga ccaatttcac gacgcttcgg
    18601 agaaccgacc atgccggttt cgcctacgga gtacggaggg aagttgtagt ggaacaggaa
    18661 ggtatcggta cgttcgccca tcagttcatc aagaacctgc gcgtcacgag cggtacccag
    18721 cgttgcagta accagtgcct gcgtttcacc gcgggtgaac agcgcagaac cgtgagtacg
    18781 cggcagcacg ccagtacgca catccagacc acggatcata tctttttcac gaccgtcgat
    18841 acgcggttcg cctgccagta cgcggctacg aacaacgttt ttctcgatag cgtgcagaat
    18901 ttcacccagt tcgttttcgt ccagggtttc gtcttcagca agcagcgtcg cgatggtttc
    18961 agatttgatg acatcaacct gcgcataacg ctcttgtttg tcggtgatgc ggtaagcatc
    19021 actcagacga gcttcagcca gtgcagcaac gcgcgcgttc agcgcttcgt ttaccggctc
    19081 cggctgccag tcccaacgcg gtttaccggc ttctttcact agttcattga tgttctgaat
    19141 aacaacctgc tgttgttcat gaccgaacac tactgcgccc agcatctgat cttcgctcag
    19201 cagttcagct tcagattcaa ccatcagtac agcggcttca gtaccggcaa caaccagatc
    19261 cagtttgctc tctttcagct cgtcctgagt cgggttcagt acgtactggt cattgatgta
    19321 acctacgcgg gcagcaccaa tcgggccatt gaacggaata ccagacagag acagtgctgc
    19381 ggaagcacca atcatcgcga cgatatccgg gttaacctgc gggttaacag aaaccacggt
    19441 ggcgataacc tgaacttcgt tgacgaagcc ttccgggaac agcgggcgaa tcgggcggtc
    19501 aatcagacgc gcgatcaggg tttcgccttc gcttggacga ccttcacgac ggaagaagct
    19561 acccgggata cgaccagcag cgtaggtacg ctcctgatag ttaacggtca gtgggaagaa
    19621 gtcctgacct ggtttggctt ttttctgtcc aacaacggta acgaataccg cggtgtcatc
    19681 catgctaacc ataacagcgg cagtagcctg acgagccatc atgccggttt ccagagtcac
    19741 ggtgtgttgg ccgtactgga atttacgaac gatcggatta agcaatgtaa tatcctttct
    19801 ctttcttaga cagtacctta cggcactggt gttaataccc gatcttctgc gcatcctcgc
    19861 gactaatgac aaccctaacc cagctctatg tgggtaaagc ctctcattag ccgcgcgaac
    19921 ctctgcaacg gaagatcatt catagcaaca atacattagt ttccagtgaa ttgctgccgt
    19981 cagcttgaaa aaaggggcca ctcaggcccc cttttctgaa actcgcaaga attagcgacg
    20041 cagacccagg cgctcgatga gctgggtgta acgtgctacg tctttacgtt tcaggtagtc
    20101 gagcagttta cgacgctgag aaaccatgcg cagcagacca cgacggctgt ggtgatcttt
    20161 tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt gcagtcagca gtgctacctg
    20221 aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac tcagaaacga ttttagctgt
    20281 tgcttcagta cttagagaca ttttaaaact ccaaagtata tagaatgaaa ggacgccgat
    20341 ctctaattca gcgatcccag tgtacgttac gcaaagtgtt aaacaattta cgcgacgtta
    20401 agcggcagta ttctactcgt agcgacctgt tatcgcaaga cggttaacat tacgccgggt
    20461 attcaaccac caggcgacga ggcgcgacgc ggccttcatc gtcaatttcg cccataccga
    20521 taaacttgcc gttctcacct tctgtaacgc gaaccagtcc ttccagcggc gcaccggatg
    20581 tacgaactgg gttaccattt ttgaagtaaa cagaagacgt taacggaaga ttcaccaccg
    20641 ggtagtccga agctggactg tccattggca tcagtaatgg atcaagtaac tccgcagccg
    20701 gaatatcctg ctgttcggct tgttcgacca gttcacgcag atgttccagg gtcaccatcc
    20761 gttcaaccgg atatttactt accgccagac gacgcaggta aataacatgc gcgccacaac
    20821 cgagtttttc acccaggtca tcaatgatgg tgcggatata agtgcctttt gagcagtgaa
    20881 tttccagctc cagctcattg ccttcgtggc gaataaacag caattcataa acggtaatcg
    20941 ggcgcgcttc acgcggtact tcaatgccct gacgcgcata ttcgtacagt tttttaccct
    21001 gatatttcag tgctgaatac atcgaaggga tctgttcgat atcgccacgg aaagtatcca
    21061 gcgccgccgc cagttgctct gcgctaaagg ttaccggacg ttcttcaacg atttgtccgt
    21121 cggcatcaga agtatcggta cgctgtccaa gacgcgcaat gacccgatag cgtttgtcgg
    21181 agtccagcag atactgggaa aacttcgtcg cttccccgag gcaaattggc aacatgcctg
    21241 ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt ggcgttataa atccgtttca
    21301 ctttttgcag cgcatcattg ctggacatac cctgcggttt atccagcaac aaaacgccgt
    21361 taatgtcgcg accgcgacga cgaggacgac tcattagtcc tccttgctgt cgtccgggtt
    21421 aacacgacgt tcttcgtcat gtttgaccac gctggtcacc aggtttgaca tgcgcatccc
    21481 ttcaaccaga gagttgtcgt agaagaaggt cagttccggc acgatacgca ggcgcatcgc
    21541 tttccccagc aggctgcgga tgaaaccaga agcttcttgc aacgctttga tgcccgcttt
    21601 aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat actttggcat acgccaggtc
    21661 gcgagacatt tcgacaccgg aaacggtggt catcatgccc aggcgaggat ctttaatttc
    21721 acgctgcagg atgagagcga tctctttttg catttcctgc gctacgcgct gcgggcgacc
    21781 aaattctttc gccataataa attctcctga caaaaaaggg gctgttagcc cctttttaaa
    21841 attaatttca ggtggaaggg ctgttcacgt tggcctgata agacgcgcca gcgtcacatc
    21901 aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg catggaaccc taaaaacctt
    21961 aagcgatggt acgttggatc tcaatgattt cgaatacttc gatcacatcg ccagtgcgga
    22021 cgtcgttgta gttcttaacg ccgataccac attccatacc gttacggact tcgttaacgt
    22081 catctttgaa gcggcgcagg gactccagct cgccttcgta gataaccacg ttgtcacgca
    22141 gaacgcggat cgggttgtga cgtttaacca caccttcggt aaccatacag cctgcgatgg
    22201 caccaaattt cggcgatttg aacacgtcac gaacttccgc cagaccgata atctgctgtt
    22261 tcagttccgg agacagcata ccgctcatcg ccgctttcac ttcgtcaatc aggttataga
    22321 tgacggagta gtaacgcaga tccaagcttt ccgcttcaat cactttacgt gcagaggcat
    22381 cagcacgtac gttgaagcca accaggatgg cgttggacgc cgcagccagg gtggcgtcgg
    22441 tttcggtgat accacctacg ccagaaccga tgatcttcac tttaacttcg tcagtagaca
    22501 gtttcagcaa ggagtcggag atcgcttcca cagaaccctg tacgtctgcc ttcaggacga
    22561 tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa catgttctcg agtttagatt
    22621 tctgctgacg cgccagttta acttcgcgga atttaccctg acgatacagt gcaacttcac
    22681 gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc cgcagccggt acgccggaca
    22741 ggccgaggat ttccaccgga atggacggac ccgcttccag cacttcctga cccagttcgt
    22801 tacgcatcgc acgaacacga ccgtattcga agccacacag aacgatatcg cccttgtgca
    22861 gagtaccttc acgtaccaga acggtagcaa ccggaccacg acctttatcg aggaaggatt
    22921 cgataaccgc accgctcgcc atacctttac gtaccgcttt cagctccaga acttccgcct
    22981 gcagcaggat agcgtccagc agttcatcga tgccggtacc cgctttcgca gatacgtgta
    23041 cgaactggct ttcaccgccc cactcttccg gcaggatgcc atactgggag agttcgttct
    23101 taacacgatc cggatcggct tctggtttat caatcttgtt cactgcaacc accaccggta
    23161 cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg cggcatcacg ccgtcgtcgg
    23221 cagcaacaac caggactacg atgtccgttg cctgcgcacc acgtgcacgc attgaagtaa
    23281 acgcggcgtg ccccggggtg tccaggaagg tgatcatgcc gttttcagtt tcaacgtggt
    23341 atgcaccaat gtgctgggta atgccgcccg cttcgccaga ggcaactttc gttgaacgaa
    23401 tgtagtccag cagagaggtt ttaccgtggt caacgtgacc catgatggtc acaaccggcg
    23461 cgcgcggttc agccgcagca cccgtgtcac ggtcgctcat taccgcctct tccagctcgt
    23521 tttcacgacg caggataact ttgtggccca tctcttcagc aaccagctgt gcggtttcct
    23581 gatcgataac ctggttgatg gttgccattg cgcccagttt catcatcgct ttgatgacct
    23641 gagagccttt aaccgccatc ttgttcgcca gttcgccaac ggtgatggtt tcgccgatca
    23701 caacgtcacg gttaacggcc tgagctggct tctggaagcc ttgctgcagg gaagaacctt
    23761 tacgttttcc gcctttaccg ccacgtactg ctgcgcgtgc ttcttcacga tcagcttttg
    23821 attcagcgtg tttgttgcct ttcttcggac gcgctgcttt cgcgttacga ccacggccac
    23881 gaccgccttc gacttcacga tcgctttcgt cttctgcctg gcgagcatgt tgagaagtag
    23941 tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt atcagtccat ttgttttctt
    24001 ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc ttcttcgagt ttacgatgcg
    24061 cttcttcttc ggctttacgc ttgagctctg cagcttcctg ctcacggcgg gctttttcag
    24121 cctgggcgtt tttagtcata tcgtcttgtt gattgctcac tttgtctttt tccgcagctt
    24181 cacgtttcgc ttgttcagca gcttcacgct tagcttgttc tgcagcctca cgttcagctt
    24241 tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg acgggcttgc tcttccgctt
    24301 cacgctgcgc ttgctcttcc gctgcaaggc gttcagcctc ttgcggatcg cgtttcacaa
    24361 aggtgcgttt cttgcggact tcgatttgta ccgatttgct ttttccaccg gtaccaggaa
    24421 tgttaagggt gctgcgtgtt ttacgttgca gcgtcaattt gtccgggcct gaatttttct
    24481 gattcaggtg gtcaatcaaa gtctgtttct cttgtgcaga cacagagtcg tcagcagact
    24541 tccggatacc tgcatcagca aattgctgta ccaggcgttc cacggaggtc tgtcgctctg
    24601 cggccagcgt tttaatcgtt acatctgtca tgctgttcct tcctgctaca gtttattacg
    24661 cttcgtcacc gaaccagcaa atattacggg cagccataat cagtgctccg gccttttcgt
    24721 cggtcaaccc ttcgatatca gccagatcat caatgccctg ttcggcgaga tcttccagcg
    24781 tacaaacgcc acgggcggcc agtttgaatg ccaaatcacg atctacccct tcaaggttca
    24841 gcagatcgtc agccggtttg ttatcaccga ggctttcttc ctgggcctgt gcaatggtgg
    24901 ccagtgcatt tttagcacgc tcgcgcagtg cttcaacggt cggctcatca aggccttcga
    24961 tttccaacag ctctttcatc ggcacatagg ccagttcttc cagcgtcgag aagccttctt
    25021 ctaccagaac agtcgcgaag tcttcgtcga tgtcgagata tttggtgaag gtgtcgatcg
    25081 ctgcgtgcgc ttccgcctga tgcttagcct gcaggtcgtc aacggtcatc acgttgagtt
    25141 cccaaccgct cagctgcgaa gccagacgca cgttctgacc gttacggcca atcgcctgcg
    25201 ccaggttacc ggcttcaacg gcgatatcca tggtgtgttt atcttcatcc accacgatag
    25261 aagcaacgtc tgccggtgcc attgcgttaa tcacgaactg cgccgggtta tcatcccaca
    25321 ggacgatatc gatacgctcg ccacccagtt cagtagacac cgcctgaaca cgcgcgccac
    25381 gcatacctac gcaagcacct accggatcga tacgtttatc gttggttttc accgcgattt
    25441 tcgcacgaga acccggatcg cgagccgctg ctttaatttc aatcacttct tcgccgattt
    25501 ctggcacttc aatacggaac agttcgatca gcatttccgg cttggaacga gtgacgaaca
    25561 gttgcgcgcc acgcgcttcc gggcgaacgg aataaagcac gccacgaacg cggtcgccag
    25621 ggcggaagtt ttcacgcggc agcatatctt cacgcaggat cacggcttca gcgttgttgc
    25681 ccagatccag agagatgttg tcgcggttta cttttttcac cacgccggtg atgatttcac
    25741 cttcgtgttc acggaactga tcaaccacca tcgcacgttc ggcttcacgc actttctgca
    25801 cgataacctg ttttgccgtc tgggtagtga tacggtcaaa ggtaacagac tcaatctgat
    25861 cttcaacgta atcgcccagg ttcaggcttt catcttcata acgtgcggct tcaagggtga
    25921 tttccttggt cggctgggtg acttcatcaa caactaacca gcgacggaag gtgtcaaaat
    25981 caccgctttt gcgatcgatc tgtacgcgga cgtcgatctc ttgttcatat tttttctttg
    26041 ttgctgtcgc cagcgcgctt tccaatgctt cgaaaatctt ctcgcgaggt agcgcctttt
    26101 cattggatac ggcttcaact acagccaaaa tttctttgtt catcgcgggc ttttcacctc
    26161 atccagacta ttaaaagtgg ggaaccaggt tcgccttctg gatattactc agcgcgaaca
    26221 cttcatcttt accttcgacg gtaactgtga tcatttcacc gtctaccgct ttgataacgc
    26281 cctgccattt acgacggttt tgtaccgcca tacggagaac cagagtcacc tcttctccga
    26341 caaaacgggc gtagtgttca gccgtgaaca gtgggcgatc gagacccggt gaggagactt
    26401 ccaggttata agcaacggtg atgggatctt caacatccag cacagcactt acctggtggc
    26461 tcacatcagc acaatcatca acattgatgc catcttcact atcaatatag atgcgcagtg
    26521 tggatgtgcg accgcgaata aattcgatgc caaccagttc aaaacccagg gcctcaactg
    26581 gcgcagtaat catctctgtt aatttttgct ctaatgtgga caagcccacc cccaagacat
    26641 aaaaaaaggg cctaaagccc agttattctg tagtcagata acaaaaaacc ccgataaatc
    26701 ggggctttat ataactgaac cctataaccg caactgcggt ctggagcact ttccagaagg
    26761 attttttcaa atcccactac gaaggccgaa gtcttcacag tatatttgaa aaaggactct
    26821 aagggaaagt ggttgcgggg gccggatttg aaccgacgac cttcgggtta tgagcccgac
    26881 gagctaccag gctgctccac cccgcgcctg aaacgtggta aattctactc gttttgggta
    26941 aaaaatgcaa atactgctgg gatttggtgt accgagacgg gacgtaaaat ctgcaggcat
    27001 tatagtgatc cacgccacat tttgtcaacg tttattgcta atcatgtgaa tgaatatcca
    27061 gttcactttc atttgttgaa tacttttgcc ttctcctgct ccccctta
//
LOCUS       DAZPEP010000043        25194 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZPEP010000043 DAZPEP010000000
VERSION     DAZPEP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.043"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            17..268
                     /locus_tag="ACHUT9_001789"
                     /pseudo
     CDS             17..268
                     /locus_tag="ACHUT9_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="involved in type II protein secretion; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..817
                     /gene="rpsJ"
                     /locus_tag="ACHUT9_001790"
     CDS             506..817
                     /gene="rpsJ"
                     /locus_tag="ACHUT9_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFR1348283.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            850..1479
                     /gene="rplC"
                     /locus_tag="ACHUT9_001791"
     CDS             850..1479
                     /gene="rplC"
                     /locus_tag="ACHUT9_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFR1348284.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1490..2095
                     /gene="rplD"
                     /locus_tag="ACHUT9_001792"
     CDS             1490..2095
                     /gene="rplD"
                     /locus_tag="ACHUT9_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFR1348285.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2092..2394
                     /gene="rplW"
                     /locus_tag="ACHUT9_001793"
     CDS             2092..2394
                     /gene="rplW"
                     /locus_tag="ACHUT9_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFR1348286.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2412..3233
                     /gene="rplB"
                     /locus_tag="ACHUT9_001794"
     CDS             2412..3233
                     /gene="rplB"
                     /locus_tag="ACHUT9_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFR1348287.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHUT9_001795"
     CDS             3250..3528
                     /gene="rpsS"
                     /locus_tag="ACHUT9_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFR1348288.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3543..3875
                     /gene="rplV"
                     /locus_tag="ACHUT9_001796"
     CDS             3543..3875
                     /gene="rplV"
                     /locus_tag="ACHUT9_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFR1348289.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHUT9_001797"
     CDS             3893..4594
                     /gene="rpsC"
                     /locus_tag="ACHUT9_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFR1348290.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4607..5017
                     /gene="rplP"
                     /locus_tag="ACHUT9_001798"
     CDS             4607..5017
                     /gene="rplP"
                     /locus_tag="ACHUT9_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFR1348291.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHUT9_001799"
     CDS             5017..5208
                     /gene="rpmC"
                     /locus_tag="ACHUT9_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFR1348292.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHUT9_001800"
     CDS             5208..5462
                     /gene="rpsQ"
                     /locus_tag="ACHUT9_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFR1348293.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5627..5998
                     /gene="rplN"
                     /locus_tag="ACHUT9_001801"
     CDS             5627..5998
                     /gene="rplN"
                     /locus_tag="ACHUT9_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFR1348294.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6009..6323
                     /gene="rplX"
                     /locus_tag="ACHUT9_001802"
     CDS             6009..6323
                     /gene="rplX"
                     /locus_tag="ACHUT9_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFR1348295.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6338..6877
                     /gene="rplE"
                     /locus_tag="ACHUT9_001803"
     CDS             6338..6877
                     /gene="rplE"
                     /locus_tag="ACHUT9_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFR1348296.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHUT9_001804"
     CDS             6892..7197
                     /gene="rpsN"
                     /locus_tag="ACHUT9_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFR1348297.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHUT9_001805"
     CDS             7231..7623
                     /gene="rpsH"
                     /locus_tag="ACHUT9_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFR1348298.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7636..8169
                     /gene="rplF"
                     /locus_tag="ACHUT9_001806"
     CDS             7636..8169
                     /gene="rplF"
                     /locus_tag="ACHUT9_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFR1348299.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8179..8532
                     /gene="rplR"
                     /locus_tag="ACHUT9_001807"
     CDS             8179..8532
                     /gene="rplR"
                     /locus_tag="ACHUT9_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFR1348300.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHUT9_001808"
     CDS             8547..9050
                     /gene="rpsE"
                     /locus_tag="ACHUT9_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFR1348301.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHUT9_001809"
     CDS             9054..9233
                     /gene="rpmD"
                     /locus_tag="ACHUT9_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFR1348302.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9237..9671
                     /gene="rplO"
                     /locus_tag="ACHUT9_001810"
     CDS             9237..9671
                     /gene="rplO"
                     /locus_tag="ACHUT9_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFR1348303.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9679..11010
                     /gene="secY"
                     /locus_tag="ACHUT9_001811"
     CDS             9679..11010
                     /gene="secY"
                     /locus_tag="ACHUT9_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFR1348304.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DATVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHUT9_001812"
     CDS             11042..11158
                     /gene="rpmJ"
                     /locus_tag="ACHUT9_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFR1348305.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHUT9_001813"
     CDS             11305..11661
                     /gene="rpsM"
                     /locus_tag="ACHUT9_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFR1348306.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHUT9_001814"
     CDS             11678..12067
                     /gene="rpsK"
                     /locus_tag="ACHUT9_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFR1348307.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHUT9_001815"
     CDS             12101..12721
                     /gene="rpsD"
                     /locus_tag="ACHUT9_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFR1348308.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHUT9_001816"
     CDS             12747..13736
                     /gene="rpoA"
                     /locus_tag="ACHUT9_001816"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFR1348309.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHUT9_001817"
     CDS             13777..14160
                     /gene="rplQ"
                     /locus_tag="ACHUT9_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFR1348310.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14267..14635
                     /locus_tag="ACHUT9_001818"
     CDS             14267..14635
                     /locus_tag="ACHUT9_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFR1348311.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            14646..15071
                     /gene="zntR"
                     /locus_tag="ACHUT9_001819"
     CDS             14646..15071
                     /gene="zntR"
                     /locus_tag="ACHUT9_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFR1348312.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15127..15345
                     /gene="arfA"
                     /locus_tag="ACHUT9_001820"
     CDS             15127..15345
                     /gene="arfA"
                     /locus_tag="ACHUT9_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFR1348313.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHUT9_001821"
     CDS             complement(15342..15752)
                     /gene="mscL"
                     /locus_tag="ACHUT9_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFR1348314.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHUT9_001822"
     CDS             complement(15882..17258)
                     /gene="trkA"
                     /locus_tag="ACHUT9_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFR1348315.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHUT9_001823"
     CDS             complement(17280..18569)
                     /gene="rsmB"
                     /locus_tag="ACHUT9_001823"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFR1348316.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQHQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHUT9_001824"
     CDS             complement(18615..19562)
                     /gene="fmt"
                     /locus_tag="ACHUT9_001824"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFR1348317.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHUT9_001825"
     CDS             complement(19577..20086)
                     /gene="def"
                     /locus_tag="ACHUT9_001825"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFR1348318.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20216..21340
                     /gene="dprA"
                     /locus_tag="ACHUT9_001826"
     CDS             20216..21340
                     /gene="dprA"
                     /locus_tag="ACHUT9_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFR1348319.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21312..21785
                     /gene="smg"
                     /locus_tag="ACHUT9_001827"
     CDS             21312..21785
                     /gene="smg"
                     /locus_tag="ACHUT9_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFR1348320.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            21814..22356
                     /locus_tag="ACHUT9_001828"
     CDS             21814..22356
                     /locus_tag="ACHUT9_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFR1348321.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHUT9_001829"
     CDS             22361..22933
                     /gene="tsaC"
                     /locus_tag="ACHUT9_001829"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFR1348322.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            22938..23756
                     /gene="aroE"
                     /locus_tag="ACHUT9_001830"
     CDS             22938..23756
                     /gene="aroE"
                     /locus_tag="ACHUT9_001830"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFR1348323.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            23753..24010
                     /locus_tag="ACHUT9_001831"
     CDS             23753..24010
                     /locus_tag="ACHUT9_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFR1348324.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(23986..24540)
                     /locus_tag="ACHUT9_001832"
     CDS             complement(23986..24540)
                     /locus_tag="ACHUT9_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFR1348325.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25013..>25194
                     /locus_tag="ACHUT9_001833"
     rRNA            25013..>25194
                     /locus_tag="ACHUT9_001833"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6425 a         5971 c         6586 g         6212 t
ORIGIN      
        1 gcttctttaa tgggcgcttt ggatagtaaa agcagaagtc actgtagagg ctgaaacgcc
       61 cgcttttaaa caatcaacac aacactattt cttcaaaaaa cagccactcc ccgtcgtcga
      121 atccgtcgag gaggaagatg acccagacgt cgcggttgag aatgctccct cttcttctga
      181 agacgaagaa aatactgtaa aagaaagcga cgagaaagcc ggtttaagag agcgtgtcaa
      241 aaatgcgctg aacgaacttg agagataacc cgaaggctgt ttactttact agcaatacgc
      301 ttgcgttcgg tggttaagta tgtataatgc gcgggcttgt cgtagttgac agcaggttca
      361 atctgaacct cagttagccg aatttggcta cctaacaatg ctcccaatcg gggagctacg
      421 taagaacggt tacactctcc catcaatcgt aatgggtctg aggagtaatc attttcgttt
      481 ataaaataat tggagctctg gtctcatgca gaaccaaaga atccgtatcc gcctgaaagc
      541 gtttgatcat cgtctgatcg atcaagcaac cgcggaaatc gtcgagactg ccaagcgcac
      601 tggtgcgcag gtccgtggtc cgatcccgct gccgacacgc aaagagcgct tcactgttct
      661 gatctccccg cacgtcaaca aagacgcgcg cgatcagtac gaaatccgta ctcacttgcg
      721 tctggttgac atcgttgagc caaccgagaa aaccgttgat gctctgatgc gtctggatct
      781 ggctgccggt gtagacgtgc agatcagcct gggttaatca ggtcattgag cgattgagag
      841 gttgaaacaa tgattggttt agtcggtaaa aaagtgggta tgacccgtat cttcacagaa
      901 gacggcgttt ctatcccagt aaccgtaatc gaagttgaag caaaccgcgt tactcaggtt
      961 aaagacctgg ctaacgatgg ctaccgtgct attcaggtga ccaccggtgc taaaaaagct
     1021 aaccgtgtga ccaagcctga agctggccac ttcgctaaag ctggcgtaga agctggccgt
     1081 ggtctgtggg aattccgcct ggctgaaggc gaagagttca ctgtaggtca gagcattagc
     1141 gttgaactgt ttgctgaggt taaaaaagtt gacgtaactg gcacctctaa aggtaaaggt
     1201 ttcgcaggta ccgttaagcg ctggaacttc cgtacccagg acgctactca cggtaactcc
     1261 ttgtctcacc gcgttccggg ttctatcggt cagaaccaga ctccgggcaa agtgttcaaa
     1321 ggcaagaaaa tggcaggtca gatgggtaac gaacgtgtaa ccgttcagag ccttgacgta
     1381 gtacgcgttg acgctgagcg caacctgctg ctggttaaag gtgctgtccc gggtgcaacc
     1441 ggtagcgacc tgatcgttaa accagctgtg aaggcgtaag gagatagcaa tggaattagt
     1501 attgaaagac gcgcagagcg cgctgactgt ttccgaaact accttcggtc gtgatttcaa
     1561 cgaagcgctg gttcaccagg ttgttgttgc ttatgcagct ggtgctcgtc agggtactcg
     1621 tgctcagaag actcgtgctg aagtaactgg ttccggtaaa aaaccgtggc gccagaaagg
     1681 caccggccgt gcgcgttctg gttctatcaa gagcccgatc tggcgttctg gtggcgtgac
     1741 ctttgctgct cgtccgcagg accacagtca aaaagttaac aagaagatgt accgcggcgc
     1801 gctgaaaagc atcctgtccg aactggtacg tcaggatcgt ctgatcgttg tcgagaagtt
     1861 ctctgtagaa gcgccgaaaa ctaagctgct ggcacagaaa ctgaaagaca tggctctgga
     1921 agatgtgctg atcatcaccg gtgagctgga cgaaaacctg ttcctggctg cgcgcaacct
     1981 gcacaaggtt gacgtacgcg atgcaactgg tatcgacccg gttagcctga tcgccttcga
     2041 caaagtcgta atgactgctg atgctgttaa gcaagttgag gagatgctgg catgattcgt
     2101 gaagaacgtc tgctgaaggt gctgcgtgca ccgcacgttt ctgaaaaagc gtctactgcg
     2161 atggaaaaat ccaacaccat cgtactcaaa gttgctaaag acgcgaccaa agcagaaatc
     2221 aaagctgctg tgcagaaact gtttgaagtc gaagtcgaag tcgttaacac cctggtagtt
     2281 aaagggaaag ttaaacgtca cggacagcgt atcggtcgtc gtagcgactg gaaaaaagct
     2341 tacgtcaccc tgaaagaagg ccagaatctg gacttcgttg gcggcgctga gtaagtcgga
     2401 ggagtaatac aatggcagtt gttaaatgta aaccgacatc tccgggtcgt cgccacgtag
     2461 ttaaagtggt taaccctgag ctgcacaagg gcaaaccttt tgctccgttg ctggaaaaaa
     2521 acagcaaatc cggtggtcgt aacaacaatg gccgtatcac cactcgtcat atcggtggtg
     2581 gccacaagca ggcttaccgt attgttgact tcaaacgcaa caaagacggt atcccggcag
     2641 ttgttgaacg tcttgagtac gatccgaacc gttccgcgaa catcgcgctg gttctgtaca
     2701 aagacggtga acgccgttac atcctggccc ctaaaggcct gaaagctggc gaccagattc
     2761 agtctggcgt tgatgctgca atcaaaccag gtaacaccct gccgatgcgc aacatcccgg
     2821 ttggttctac tgttcataac gtagaaatga aaccaggtaa aggcggtcag ctggcacgtt
     2881 ccgctggtac ttacgttcag atcgttgctc gtgatggtgc ttatgtcacc ctgcgtctgc
     2941 gttctggtga aatgcgtaaa gtagaagcag actgccgtgc aactctgggc gaagttggca
     3001 atgctgagca tatgctgcgc gttctgggta aagcaggtgc tgcacgctgg cgtggtgttc
     3061 gtccgaccgt tcgcggtacc gcgatgaacc cggtagacca cccacatggt ggtggtgaag
     3121 gtcgtaactt tggtaagcac ccggtaactc cgtggggcgt tcagaccaaa ggtaagaaga
     3181 cccgcagcaa caagcgtact gataaattca tcgtacgtcg ccgtagcaaa taattttaga
     3241 ggataagcca tgccacgttc tctcaagaaa ggtcctttta ttgacctgca cttgctgaag
     3301 aaggtagaga aagcggtgga aagcggagac aagaagcccc tgcgcacttg gtcccgtcgt
     3361 tcaacgatct ttcctaacat gatcggtttg accatcgctg tccataatgg tcgtcagcac
     3421 gttccggtat ttgtgaccga cgaaatggtc ggtcacaaac tgggtgaatt cgcaccgact
     3481 cgtacttatc gcggccacgc tgctgataaa aaagcgaaga agaaataagg taggaggaag
     3541 agatggaaac tatcgctaaa catcgccatg ctcgttcttc tgctcagaag gttcgccttg
     3601 ttgctgacct gattcgcggt aagaaagtgt cgcaggctct ggatattttg acctacacca
     3661 acaagaaagc ggctgtactg gtcaagaaag ttctggaatc tgccattgct aacgctgaac
     3721 acaacgatgg cgctgacatt gacgatctga aagttacgaa aattttcgta gacgaaggcc
     3781 cgagcatgaa gcgcattatg ccgcgtgcaa aaggtcgtgc agatcgcatc ctgaagcgca
     3841 ccagccacat cactgtggtt gtgtccgatc gctgagactc tggagactag caatgggtca
     3901 gaaagtacat cctaatggta ttcgcttggg tattgtaaag ccatggaact ctacctggtt
     3961 tgcgaacacc aaagaattcg ctgacaacct ggacagcgat tttaaagtac gtcagtacct
     4021 gactaaggaa ctggctaaag cgtccgtatc tcgtatcgtt atcgagcgtc cggctaagag
     4081 catccgtgta accattcaca ctgctcgccc gggtatcgtt atcggtaaaa aaggtgaaga
     4141 cgtagaaaaa ctgcgtaagg tcgtagcgga catcgctggc gttcctgcac agatcaacat
     4201 cgccgaagtt cgtaagcctg aactggacgc aaaactggtt gctgacagca tcacttctca
     4261 gctggaacgt cgcgttatgt tccgtcgtgc tatgaagcgt gctgtacaga acgcaatgcg
     4321 tctgggcgct aaaggtatta aagttgaagt tagcggccgt ctgggcggcg cggaaatcgc
     4381 acgtaccgaa tggtaccgcg aaggtcgcgt accgctgcac actctgcgtg ctgacatcga
     4441 ctacaacacc tctgaagcgc acaccactta cggtgtaatc ggcgttaaag tgtggatctt
     4501 caaaggcgag atcctgggtg gtatggctgc tgttgaacaa ccggaaaaac cggctgctca
     4561 gcctaaaaag cagcagcgta aaggccgtaa ataaggagcg tcgctgatgt tacaaccaaa
     4621 gcgtacaaaa ttccgtaaaa tgcacaaagg ccgtaaccgc ggtctggcgc agggtacgga
     4681 tgttagcttc ggcagcttcg gtctgaaagc tgttggccgt ggtcgtctga ctgcccgtca
     4741 gatcgaagca gcacgtcgtg ctatgacccg tgcagttaag cgtcaaggta agatctggat
     4801 ccgtgtgttc ccggacaaac cgatcactga aaagccgctg gcagtgcgta tgggtaaagg
     4861 taaaggtaac gtggagtatt gggttgcctt gattcagccg ggtaaagtcc tgtatgaaat
     4921 ggacggtgtt ccggaagagc tggcccgtga agcattcaag ctggcagcag cgaaactgcc
     4981 gattaaaacc acctttgtaa ctaagacggt gatgtaatga aagcaaaaga gctgcgtgag
     5041 aagagcgttg aagagctgaa caccgagctg ctgaacctgc tgcgtgagca gttcaacctg
     5101 cgtatgcagg ctgcaagtgg ccagctgcaa cagtctcacc tgttgaagca agtgcgtcgc
     5161 gatgtcgcac gcgttaagac tttactgaac gagaaggcgg gtgcgtaatg accgataaaa
     5221 tccgtactct gcaaggtcgc gttgttagcg acaaaatgga gaaatccatt gttgttgcta
     5281 tcgaacgttt tgtgaaacac ccgatctacg gtaaattcat caagcgtacg accaaactgc
     5341 acgtacatga cgagaacaac gaatgcggta tcggtgacgt ggttgaaatc cgcgaatgcc
     5401 gtccgctgtc caagactaaa tcctggacgc tggttcgcgt tgtagagaaa gcggttctgt
     5461 aatacagtac actctctcga tacgaataaa cggctcagaa atgagccgtt tattttttct
     5521 acccatatcc ttgaagcggt gttataatgc cgcgccctcg atatggggat ttttaacgac
     5581 ctgattttcg ggtctcagta gtagttgaca ttagcggagc actaaaatga tccaagaaca
     5641 gactatgctg aacgtcgccg acaactccgg tgcacgtcgc gtaatgtgta tcaaggttct
     5701 gggtggctcg caccgtcgct acgcaggcgt aggcgacatc atcaagatca ccatcaaaga
     5761 agcaattccg cgtggtaagg tcaaaaaagg tgatgtgctg aaggcggtag tggtgcgcac
     5821 caagaagggt gttcgtcgcc cggacggttc tgtcattcgc ttcgatggta atgcttgtgt
     5881 tcttctgaac aacaacagcg agcagcctat cggtacgcgt atttttgggc cggtaactcg
     5941 tgagcttcgt agtgagaagt tcatgaaaat tatctctctg gcaccagaag tactctaagg
     6001 agcgaatcat ggcagcgaaa atccgtcgtg atgacgaagt tatcgtgtta accggtaaag
     6061 ataaaggtaa acgcggtaaa gttaagaatg tcctgtcttc cggcaaggtc attgttgaag
     6121 gtatcaacct ggttaagaaa catcagaagc cggttccggc cctgaaccaa ccgggtggca
     6181 tcgttgaaaa agaagccgct attcaggttt ccaacgtagc aatcttcaat gcggcaaccg
     6241 gcaaggctga ccgtgtaggc tttagattcg aagacggcaa aaaagtccgt ttcttcaagt
     6301 ctaacagcga aactatcaag taatttggag tagtacgatg gcgaaactgc atgattacta
     6361 caaagacgaa gtagttaaaa aactcatgac tgagtttaac tacaattctg tcatgcaagt
     6421 ccctcgggtc gagaagatca ccctgaacat gggtgttggt gaagcgatcg ctgacaaaaa
     6481 actgctggat aacgcagcag cagacctggc agcaatctcc ggtcaaaaac cgctgatcac
     6541 caaagcacgc aaatctgttg caggcttcaa aatccgtcag ggctatccga tcggctgtaa
     6601 agtaactctg cgtggcgaac gcatgtggga gttctttgag cgcctgatca ctattgctgt
     6661 acctcgtatt cgtgacttcc gtggcctgtc cgctaagtct ttcgacggtc gtggtaacta
     6721 cagcatgggt gtccgtgagc agatcatctt cccagaaatc gactacgata aagtcgaccg
     6781 cgttcgtggt ttggatatta ccattaccac tactgcgaaa tctgacgaag aaggccgcgc
     6841 tctgctggct gcctttgact tcccgttccg caagtaaggt agggttacta aatggctaag
     6901 caatcaatga aagcacgcga agtaaaacgc gtagctttag ctgataaata cttcgcgaaa
     6961 cgcgctgaac tgaaagcgat catctctgat gtgaacgctt ccgacgaaga tcgttggaac
     7021 gctgttctca agctgcagac tctgccgcgt gattccagcc cgtctcgtca gcgtaaccgc
     7081 tgccgtcaaa caggtcgccc gcatggtttc ctgcggaagt tcgggttgag ccgtattaag
     7141 gtccgtgaag ccgctatgcg cggtgaaatc ccgggtctga aaaaggctag ctggtaattg
     7201 tcaccaattg aatcacggga gtaaagacag atgagcatgc aagatccgat cgcggatatg
     7261 ctgacccgta tccgtaacgg tcaggccgcg aacaaagctg cggtcaccat gccttcctcc
     7321 aagctgaaag tggcaatcgc caacgtgctg aaggaagaag gttttattga agattttaaa
     7381 gttgaaggcg acaccaagcc tgaactggaa cttactctga agtatttcca gggcaaagct
     7441 gttgtagaaa gcattcagcg tgtcagccgc ccaggtctgc gcatctataa acgtaaagat
     7501 gagctgccga aagttatggc gggtctgggt atcgcagttg tttctacctc taaaggtgtt
     7561 atgactgatc gtgcagcgcg ccaggctggt cttggtggcg aaattatctg ctacgtagcc
     7621 taatcggagg aaaaaatgtc tcgtgttgct aaagcaccgg tcgttgttcc tgccggcgtt
     7681 gacgtaaaaa tcaacggtca ggttattacg atcaaaggta aaaacggcga gctgactcgt
     7741 actctcaacg atgctgttga agttaaacat gcagataata ccctgacctt cggtccgcgt
     7801 gatggttacg cagacggttg ggcacaggct ggtaccgcgc gtgccctgct gaactcaatg
     7861 gttatcggtg ttaccgaagg cttcactaag aagctgcagc tggttggtgt aggttaccgt
     7921 gcagcggtta aaggcaatgt gattaacctg tctctgggtt tctctcatcc tgttgaccat
     7981 cagctgcctg cgggtatcac tgctgaatgt ccgactcaga ctgaaatcgt gctgaaaggc
     8041 gttgataagc aggtgatcgg ccaggttgca gcggatctgc gcgcctaccg tcgtcctgag
     8101 ccttataaag gcaagggtgt tcgttacgcc gacgaagtcg tgcgtaccaa agaggctaag
     8161 aagaagtaag gtaacactat ggataagaaa tttgctcgta tccgtcgtgc gacccgcgca
     8221 cgccgcaagc tccaggagct gggcgcaact cgcctggtgg tacatcgtac cccgcgtcac
     8281 atttacgcac aggtaattgc accgaacggt tctgaagttc tggtagctgc ttctactgta
     8341 gaaaaagcta tcgctgaaca actgaagtac accggtaaca aagacgcggc tgcagctgtg
     8401 ggtaaagctg tcgctgaacg cgctctggaa aaaggcatca aagatgtatc ctttgaccgt
     8461 tccgggttcc aatatcatgg tcgtgtccag gcactggcag atgctgcccg tgaagctggc
     8521 cttcagttct aaggtagagg tgtaagatgg ctcacatcga aaaacaagct ggcgaactgc
     8581 aggaaaagct gatcgcggta aaccgcgtat ctaaaaccgt taaaggtggt cgtattttct
     8641 ccttcacagc tctgactgta gttggcgatg gtaacggtcg cgttggtttt ggttacggta
     8701 aagcgcgtga agttccagca gcgatccaga aagcgatgga aaaagcccgt cgcaatatga
     8761 ttaacgtcgc gctgaataac ggcactctgc aacaccctgt taaaggtgtt cacacgggtt
     8821 ctcgcgtatt catgcagccg gcttccgaag gtaccggtat catcgccggt ggtgcaatgc
     8881 gcgccgttct ggaagtcgct ggggttcata acgttctggc taaagcatat ggttccacca
     8941 acccgatcaa cgtggttcgt gcaactattg atggcctgga aaatatgaat tctccagaaa
     9001 tggtcgctgc caagcgtggt aaatccgttg aagaaattct ggggaaataa accatggcaa
     9061 agactattaa aattactcaa acccgcagtg caatcggtcg tctgccgaaa cacaaggcaa
     9121 cgctgcttgg cctgggtctg cgtcgtattg gtcacaccgt agagcgcgag gatactcctg
     9181 ctattcgcgg tatgatcaac gcggtttcct tcatggttaa agttgaggag taagagatgc
     9241 gtttaaatac tctgtctccg gccgaaggct ccaaaaaggc gggtaaacgc ctgggtcgtg
     9301 gtatcggttc tggcctcggt aaaaccggtg gtcgtggtca caaaggtcag aagtctcgtt
     9361 ctggcggtgg cgtacgtcgc ggtttcgagg gtggtcagat gcctctgtac cgtcgtctgc
     9421 cgaaattcgg cttcacttct cgtaaagcag cgattacagc cgaagttcgt ctgtctgacc
     9481 tggctaaagt agaaggcggt gttgtagacc tgaacacgct gaaagcggct aacattatcg
     9541 gtatccagat cgagttcgcg aaagtgatcc tggctggcga agtaacgact ccggtaactg
     9601 ttcgtggcct gcgtgttact aaaggcgctc gtgctgctat cgaagctgct ggcggtaaaa
     9661 tcgaggaata agtagcagat ggctaaacaa ccgggattag attttcaaag tgccaaaggt
     9721 ggcttaggcg agctgaaacg cagactgctg tttgttatcg gtgcgctgat tgtgttccgt
     9781 attggctctt ttattccgat ccctggtatt gatgccactg tacttgccaa actgcttgag
     9841 caacagcgag gcaccatcat tgagatgttt aacatgttct ctggtggtgc tctcagccgt
     9901 gcttctatct ttgctctggg gatcatgccg tatatttcgg cgtcgatcat tatccagctg
     9961 ctgacggtgg ttcacccaac gttggcggaa attaagaaag aaggggagtc tggtcgtcgt
    10021 aagatcagcc agtacacccg ctacggtact ctggtgctgg caatattcca gtcgatcggt
    10081 attgctactg gtctgccgaa tatgcctggt atgcaaggcc tggtgattaa cccgggcttt
    10141 gcattctact tcaccgctgt tgtaagtctg gtcacaggaa cgatgttcct gatgtggttg
    10201 ggcgaacaga ttactgaacg aggtatcggc aacggtattt caatcattat cttcgtcggt
    10261 attgtcgcgg gactcccgcc agccattgcc catactatcg agcaagcgcg tcaaggcgac
    10321 ctgcacttcc tcgtgttgct gttggttgca gtattagtat ttgcagtgac gttctttgtt
    10381 gtatttgttg agcgtggtca acgccgcatt gtggtaaact acgcgaaacg tcagcaaggt
    10441 cgtcgtgttt atgctgcaca gagcacacat ttaccgctga aagtgaatat ggcgggggta
    10501 atcccggcaa tcttcgcttc cagtattatt ctgttcccgg cgaccatcgc gtcatggttc
    10561 gggggcggta ctggttggaa ctggctgaca acaatttcgc tgtatttgca gcctgggcaa
    10621 ccgctttatg tgttactcta tgcgtctgca atcatcttct tctgtttctt ctacacggcg
    10681 ttggttttca acccgcgtga aacagcagat aacctgaaga agtccggtgc atttgtacca
    10741 ggaattcgtc cgggagagca aacggcgaag tatatcgata aagtaatgac ccgcctgacc
    10801 ctggttggtg cgctgtatat tacctttatc tgcctgatcc cggagttcat gcgtgatgca
    10861 atgaaagtac cgttctactt cggtgggacc tcactgctta tcgttgttgt cgtgattatg
    10921 gactttatgg ctcaagtgca aactctgatg atgtccagtc agtatgagtc tgcattgaag
    10981 aaggcgaacc tgaaaggcta cggccgataa ttggtcgccc gagaagttac ggagagtaaa
    11041 aatgaaagtt cgtgcttccg tcaagaaatt atgccgtaac tgcaaaatcg ttaagcgtga
    11101 tggtgtcatc cgtgtgattt gcagtgccga gccgaagcat aaacagcgcc aaggctgatt
    11161 ttttcgcata tttttcttgc aaagttgggt tgagctggct agattagcca gccaatcttt
    11221 tgtatgtctg tgcgtttcca tttgagtatc ctgaaaacgg gcttttcagc atggaacgta
    11281 catattaaat agtaggagtg catagtggcc cgtatagcag gcattaacat tcctgatcat
    11341 aagcatgccg taatcgcatt aacttcgatt tatggcgtcg gcaagacccg ttctaaagcc
    11401 atcctggctg cagcgggtat cgctgaagat gttaagatca gtgagctgtc tgaaggacaa
    11461 atcgacacgc tgcgtgacga agttgccaaa tttgtcgttg aaggtgatct gcgccgtgaa
    11521 atcagcatga gcatcaagcg cctgatggat cttggttgct atcgcggttt gcgtcatcgt
    11581 cgtggtctcc cggttcgcgg tcagcgtacc aagaccaacg cacgtacccg taagggtccg
    11641 cgcaaaccga tcaagaaata atcggggtga ttgaataatg gcaaaggcac caattcgtgc
    11701 acgtaaacgt gtaagaaaac aagtctctga cggcgtggct catatccatg cttctttcaa
    11761 caacaccatc gtgactatca ctgatcgtca gggtaacgcg ttgggttggg caacagccgg
    11821 tggttccggt ttccgtggtt ctcgcaaatc cactccgttt gcagctcagg ttgcagcaga
    11881 gcgttgcgct gacgccgtga aagaatacgg catcaagaat ctggaagtta tggttaaagg
    11941 tccgggtcca ggccgcgaat ctactattcg tgctctgaac gccgcaggtt tccgcatcac
    12001 taacattact gatgtgactc cgatccctca taacggttgt cgtccgccga aaaaacgtcg
    12061 cgtataacgc ctcgttttcc aggtttgttg gagaaagaaa atggcaagat atttgggtcc
    12121 taagctcaag ctgagccgtc gtgagggcac cgacttattc cttaagtctg gcgttcgcgc
    12181 gatcgatacc aagtgtaaaa ttgaacaagc tcctggccag cacggtgcgc gtaaaccgcg
    12241 tctgtctgac tatggtgtgc agttgcgtga aaagcaaaaa gttcgccgta tctatggtgt
    12301 gctggagcgt cagttccgta actactacaa agaagcagca cgtctgaaag gcaacaccgg
    12361 tgaaaacctg ttggctctgc tggaaggtcg tctggacaac gttgtatacc gtatgggctt
    12421 cggtgccact cgtgcagaag cacgtcagct ggttagccat aaagcaatta tggtaaacgg
    12481 tcgtgttgtt aacatcgctt cttatcaggt tagtccgaat gacgttgtaa gcattcgtga
    12541 gaaagcgaag aagcagtctc gcgtgaaagc cgctctggag ctggctgagc agcgtgaaaa
    12601 gccaacctgg ctggaagttg atgctggcaa gatggaaggt acgtttaagc gtaagccgga
    12661 gcgttctgat ctgtctgcgg acattaacga acacctgatc gtcgagcttt actccaagta
    12721 aagcttagta ccaaagagag gacacaatgc agggttctgt gacagagttt ctaaaaccgc
    12781 gcctggttga tatcgagcaa gtgagttcga cgcacgccaa ggtgaccctt gagcctttag
    12841 agcgtggctt tggccatact ctgggtaacg cactgcgccg tattctgctc tcatcgatgc
    12901 cgggttgcgc ggtgaccgag gttgagattg atggtgtact acatgagtac agcaccaaag
    12961 aaggcgttca ggaagatatc ctggaaatcc tgctcaacct gaaagggctg gcggtgagag
    13021 ttcagggcaa agatgaagtt attcttacct tgaataaatc tggcattggc cctgtgactg
    13081 cagccgatat cacccacgac ggtgatgtcg aaatcgtcaa gccgcagcac gtgatctgcc
    13141 acctgaccga tgagaacgcg tctattagca tgcgtatcaa agttcagcgc ggtcgtggtt
    13201 atgtgccggc ttctacccga attcattcgg aagaagatga gcgcccaatc ggccgtctgc
    13261 tggtcgacgc atgctacagc cctgtggagc gtattgccta caatgttgaa gcagcgcgtg
    13321 tagaacagcg taccgacctg gacaagctgg tcatcgaaat ggaaaccaac ggcacaatcg
    13381 atcctgaaga ggcgattcgt cgtgcggcaa ccattctggc tgaacaactg gaagctttcg
    13441 ttgacttacg tgatgtacgt cagcctgaag tgaaagaaga gaaaccagag ttcgatccga
    13501 tcctgctgcg ccctgttgac gatctggaat tgactgtccg ctctgctaac tgccttaaag
    13561 cagaagctat ccactatatc ggtgatctgg tacagcgtac cgaggttgag ctccttaaaa
    13621 cgcctaacct tggtaaaaaa tctcttactg agattaaaga cgtgctggct tcccgtggac
    13681 tgtctctggg catgcgcctg gaaaactggc caccggcaag catcgctgac gagtaaccgg
    13741 atcacaggtt aaggttttac tgagaaggat aaggtcatgc gccatcgtaa gagtggtcgt
    13801 caactgaacc gcaacagcag ccatcgccag gctatgttcc gcaatatggc aggttcactg
    13861 gttcgtcatg aaatcatcaa gacgactctg cctaaagcga aagagctgcg ccgcgtagtt
    13921 gagccgctga ttactcttgc caagactgat agcgttgcta atcgtcgtct ggcattcgcc
    13981 cgtactcgtg ataacgagat cgtggcaaaa ctgtttaacg aactgggccc gcgtttcgcg
    14041 agccgtgccg gtggttacac tcgtattctg aagtgtggct tccgtgcagg cgacaacgcg
    14101 ccgatggctt acatcgagct ggttgatcgt tcagagaaag cagaagctgc tgcagagtaa
    14161 tctgaagcaa tgtaaaaaaa cccgccccgg cgggtttttt tatacccgta gtatccccac
    14221 ttatctacaa tagctgtact ctttttgttc atcccctgga gtatttatgt ggttacttga
    14281 ccagtgggca gagcgccata tagcagaagc gcaagcgaaa ggtgagtttg ataacctggc
    14341 aggtagcggc gaaccattga tactggatga tgattctcac gtgccaccgg aattacgtgc
    14401 ggggtatcgc ttgctgaaga atgccggttg cttaccgcca gaacttgagc aacggagaga
    14461 agcaattcag cttctggata ttctcaaagg tatccgtcac gatgatccgc aatatcaaga
    14521 ggttagccgt cgattgtcat tactggaatt gaagctgcga caagctggat tgagtaccga
    14581 ttttttacgc ggcgattatg ctgacaagtt gttggacaaa atcaacgata actagtggag
    14641 tatgtatgta tcgcattggt gagctggcaa aaatggcgga agtaacacct gacacgattc
    14701 gttattacga aaaacagcag atgatggagc atgaagtgcg tactgaaggc gggtttcgcc
    14761 tgtataccga aagcgatctc cagcgattga aatttatccg ccatgccaga caactaggtt
    14821 tcagtctgga gtcgatccgc gagttgctgt cgatccgcat cgatcctgaa caccatacct
    14881 gtcaggagtc aaaaggcatt gtgcaggaaa gattgcagga agtcgaagca cggatagccg
    14941 agttgcagag tatgcagcgt tccttgcaac gccttaacga tgcctgttgt gggaccgccc
    15001 atagcagtgt ttattgctcg attcttgaag ctcttgaaca aggggcgagt ggcgttaaga
    15061 gtggctgttg attttttgca ctgacaggat tacactcgcg ccgttaaata accaactgga
    15121 gtttttatga gccgatatca gcatactaaa gggcagataa aggataatgc gatagaagca
    15181 ttactacatg atcccttatt ccgacagcga gtagagaaaa ataagaaagg gaaaggcagt
    15241 tacatgcgaa aaggaaaaca tggcaatcgg gggaactggg aggccagtgg caagaaagta
    15301 aatcactttt ttaccactgg tcttctgctt tcaggcgctt gttaagagcg gttattctgc
    15361 tctttcagca aatcacgaat ttctgccagt aatacttctt ctttagttgg tgcaggtgcg
    15421 gcagctggtt cttctttttt ccgattcagt ttgttgatta gcttaatcgc cataaagatg
    15481 gcaaaggcca caatcagaaa atcaaagacg ttttgaatga agacaccgta atgcatcaca
    15541 acagcaggga tatccccctg cgcatcgcgt agcgtgacag caaactgttt aaagtcgatc
    15601 ccgccaatta ataagcccag aggtggcatg atgatatcgg caaccagtga agatacaatc
    15661 ttcccgaatg ccgcaccgat aatgacaccc accgccaaat ccaccacgtt cccgcgcatc
    15721 gcaaattcgc gaaattcttt aataatgctc atgttattct ccctatgaag ccgacaacca
    15781 taagtctaac aaatgttaag ccattttcct gccaggaatg aatttaatta atccttgata
    15841 atcaaaggga aataataagg cgtcattaga cgccttatta attacaagaa gaaagggctt
    15901 ggctggaaga gtctttcgac gtcggtaata aactttttat ctgtgaggaa cataattacg
    15961 tgatcgcctt gctcaatgcg cagattgtca ttggcaatca tcacgtcgtt tccacgtacc
    16021 accgctccaa taatcgttcc tggcggtagc ttgatttcgt caatgactct gccgacaacg
    16081 cgtgaggtgc tttcatcacc gtgagcaacg gcttcaatag cttctgctac gccgcggcgc
    16141 aatgaggaaa caccaacaat atctgctttt cgcacatggc taagcaacgc agaaatagtt
    16201 gcttgttgtg gtgaaattgc aatatcgatc acgctcccct gaaccagatc cacataagcg
    16261 cgacgctgga tcaataccat cacctttttc gcacccatac gtttggcaag catggcggac
    16321 atgatattgg cctcgtcatc gttggtgacg gcaataaaca gatcaacttg atcgatatgt
    16381 tcttcggcca gcagttcttg atccgacgca tcaccaaaaa agacgatcgt attctgcaac
    16441 ttttccgcca gttcggcagc gcgctgctga ttacgttcga tgagtttaac gctgtaatct
    16501 ttttccagac gacgcgccag ccctgcaccg atattaccgc caccaaccag catgatccgc
    16561 ttatacggtt tttccagtcg ctgtaattca ctcatcaccg cgcggatatg ctgtgaagcg
    16621 gcaataaaga acacttcatc accagcttca acaatggtcg aaccttgtgg acgaatgggg
    16681 cgatcgtggc ggaaaattgc tgccacacga gtatcgatat gtggcatatg ttcgcgcatg
    16741 gtcgaaagtg cattaccaat cagcgggccg ccataatagg ctttaactac agcgaggctg
    16801 actttaccct cagcgaagtt caccacctgc aatgcgcctg gatactcaat cagtcggtaa
    16861 atattatcga taaccaactg ctctggtgcg atcagatgat caatcggtac agcatctgaa
    16921 tgaaatagct tatcggcatc gcgtacgtag tctggtgagc ggatgcgagc gatgcgatta
    16981 ggggtgttga aaagtgagta ggctacctgg caggcaacca tattggtttc atctgaactg
    17041 gttacagcaa ccagcatatc ggcgtcgtcg gcacctgcct cccgcaatac gcgtggatga
    17101 gagccatgcc cctgcacgac tcgcaggtca aatttatcct gcaaggtccg cagacgctca
    17161 ccgttggtat cgacaacagt aatatcgttg ttctcgccaa ccaggttttc cgccagtgtg
    17221 ccgccaacct ggccggcacc cagaatgata attttcatca gtctcgaccc gttatctcat
    17281 cactttttga ttagcttagc gtaaaagaag ccgtcgccct cttcggcacc aggcagattt
    17341 tgtttacccg gttgctctgg tgttcctgtt tcgcaaagtt cggcatcagc ggtacgttgc
    17401 aaaaaagctt taatctgcag gctattctct tccggtaaca ccgaacaggt ggcatagacc
    17461 agggttccac cggattttaa atgcggccaa atggcgtcga gaatttcaga ctgcaattgc
    17521 gcgagttccg ggatatcgcg atcgcgacgt aaccatttaa tatccggatg gcgacgaatc
    17581 acccccgttg ctgaacaagg cgcatctaat aaaatgcgat caaactgttg ctcgccacac
    17641 cattgggaag ggtaacggcc atcaccttgt ttcacggtcg ccttcatacc aaggcgtttt
    17701 aaattgtcgt gaacgcgaga gaggcgctgt tcgtcaatat caaccgcaac aacctgcgct
    17761 tctggtgcca cctcaaggat atgcgttgtt ttaccgccgg gggcggcaca aagatccaga
    17821 atgtgttcac cgttttgtgg cgcaagccag gtcatgcaac cttgtgctga tgcatcctga
    17881 acggtaaccc atccgtcttc aaaaccaggt agcgcatgaa caggtgcagg tgtttccagc
    17941 cgtacagcat cagggtaatc cgcatgcggg aaacctttca ttcctgcttc atccaacaat
    18001 gcaagccagc tgtcgcggga atgatgcgta cgattaacgc gcagccacat tggcggacgc
    18061 tggttattgg cttcgacgat ggattgccac tgctctggat acgctttttg cagacgcttc
    18121 agcaaccagg aaggatgcag ataacgtgca tcactggtat taaactcggc taataactct
    18181 tcttgctgat gctggaactg gcgtaataca ccgttaatca accctttaag ttgcggacgc
    18241 ttaattgcga tagcgccttc aaccgtttca gccagcgcag catgaggtgg aatgcgggta
    18301 taaagcagtt gatacaaacc aaccataatc aggtaatgca cagtgcgctg tttgccggtc
    18361 atcggacggg ccattaactt attaatcagc cagtctaact gcgaaagcgt acgcagtacg
    18421 ccaaagcaca actcttgaag aagtgctttg tctttatcag aaactttttg ctggagcggt
    18481 ggcagaatgt tgcttaatga ttgcccttgc tcgacgactt gttcaacggc ctgggccgcc
    18541 atgctacgta aattacgttg ttttttcata agtataaaaa tgcccggtaa gaccgggctt
    18601 agaagagtgg actatcaggc cagacggttg cccggaacaa accattcccg acgagaattc
    18661 aggaggtctt gcgcgctcat cgctttctta cccgcaggtt gtaacgagag caggttcagg
    18721 atgccatcac cagtcgcaac ctgaatgcct tgtttgttgg cttcaaggat cgttcctggt
    18781 gcagcgttgg ttgccgtatc aatgaccgac gctttccaga ctttaaccgg ttgtccttca
    18841 atttccagcc agctcattgg ccatgggttg aaagcgcgaa tgcagcgttc aagctgcgct
    18901 gccgaaagcg accagtcaat acgcgcttct tctttactca acttctcggc gtaagtgaca
    18961 agagtttcgt cctgaacttc tggtttcgcc gtgccgtctg ctagttgttt caacgtggtg
    19021 attagccctt gtgggccaag ctctgccagc ttgtcgtaca gcgtaccact ggtatcttct
    19081 gcagtaatcg ggcaggagag cttatagagc atatcaccgg tgtctaaacc gacatccatt
    19141 tgcataatgg tcacaccagt ttctgcatca cccgcccata gtgagcgttg gattggtgca
    19201 gcaccgcgcc agcgtggcag cagtgaacca tgaacgttga tacagccaag acgcggcatc
    19261 tccagcactg ctttaggcag aattaagcca taggcgacga cgaccataac atcagcctgc
    19321 agatcggcga ccagttgctg gctttcttgt ggacgcaggg aaacaggttg aaaaacgggc
    19381 agaccttttt cctcagccag aactttaacc gggctgggca tcagtttttt accgcgtcct
    19441 gccggtcggt ctggctgggt gaacacgcca acgacgttat gaccagaaga caacagcgcg
    19501 tcgagatgac gcgctgcaaa gtcaggtgta cccgcaaaaa taatacgtag tgattctgac
    19561 acgttagttc ttatccttaa gcccgggctt tcagacgatc tagtttttca actttctgac
    19621 gaatacgttg ttgtttcagc ggtgacagat aatccataaa cagtttgccg accaggtgat
    19681 ccatctcatg ctgaatacag atggctaaca gcccctctgc ttccagttca aatggtttac
    19741 cgtcgcggtc aagggcgcga attttaactt tctctgcgcg cggcactaaa gcacgttgtt
    19801 cagggatcga caggcaacct tcttcaatgc ctgtttcgcc gcttttttct aaaagctccg
    19861 gattgattaa caccagccgt tcgtcacggt tttccgaaac atcaataaca atgatacgtt
    19921 gatggatatc aacctgggtt gccgccaggc caataccttc ttctgcgtac atcgtctcga
    19981 acatatcatc gacgatacgc tgtatttctg cattcacttc ttctaccggt ttagcaactt
    20041 tgcgaagccg ctcgtccgga atatgtaaca cttgcaaaac tgacataaat ctccagagat
    20101 gtgttcaggg gttagaaaga ttatttcttc tattctagac aaatccccct ctgattgaca
    20161 gcatcactga ccaatcgcaa agattgctaa ggctgcttat ggcagggaga taaggatggt
    20221 cgatacagaa atttggctgc gtttaatgag tatcagcagc ttgtacggcg atgatatggt
    20281 ccgtatagct cactggctgg caaaacagtc gcaaattgat gcggttggat tgcagcaaac
    20341 agggcttaca ttgcggcagg cacaacgctt tctttcattt ccgcgaaaga gtatcgaaag
    20401 ctcactttgt tggttggagc aacccaacca tcatttaatc cctgcggaca gcgaatttta
    20461 tcctcctcaa cttctggtga cgacagatta ccccggcgca ctgtttgttg aaggagaact
    20521 gcacgcgctg cattcatttc agcttgccgt agtggggagt cgggcgcatt catggtatgg
    20581 cgagcgatgg ggacggttat tttgcgaaac tctggcgacg cgtggagtga caattacgag
    20641 tggactggcg cgtggaatcg atggtgtggc gcataaagct gccttacagg taaatggcgt
    20701 cagcattgct gtattgggga atggacttaa taccattcat ccccgccgcc atgcccgact
    20761 ggctgccagt ctgcttgaac atggcggagc tctcgtctcg gaatttcccc tcgatgttcc
    20821 accccttgct tacaatttcc cacgaagaaa tcgcattatc agtggtctaa gtaaaggtgt
    20881 actggtggtg gaagcggctt tgcgcagtgg ttcgctggtg acagcacgtt gtgcgcttga
    20941 gcagaggcgt gaagtttttg ccttgccagg tccaataggg aatccgggaa gcgaagggcc
    21001 tcactggtta ataaaacaag gtgcgattct tgtgacggaa ccggaagaaa ttctggaaaa
    21061 cttgcaattt ggattgcact ggttgccaga cgcccctgaa aattcatttt attcaccaga
    21121 tcagcaagac gtggcattgc catttcctga gctcctggct aacgtaggag atgaggtaac
    21181 acctgttgac gtcgtcgctg aacgtgccgg ccaacctgtg ccagaggtag ttactcaact
    21241 actcgaactg gagttagcag gatggatcgc agctgtaccc ggcggctatg tccgattgag
    21301 gagggcatgc catgttcgac gtactaatgt atttgtttga aacctatatt cacactgaag
    21361 ctgagttgcg tgtggatcaa gacaaacttg aacaggatct taccgacgca ggttttgagc
    21421 gagaagatat ctacaatgcc ctgctatggc tggaaaaact tgctgattat caggaagggt
    21481 tggcagaacc gatgcaactg gcctcggatc ctctctccat gcgtatttat acaccggaag
    21541 agtgtgaacg actggatgcc agctgccgtg ggtttctgct tttccttgag cagattcagg
    21601 tgctcaacct tgaaactcgt gaaatggtga tagagcgagt gctcgcgctg gataacgcag
    21661 agttcgaact ggatgatctg aaatgggtga tcctgatggt gttgttcaac atcccggggt
    21721 gcgaaaatgc gtaccagcaa atggaagaat tactctttga agtgaatgaa ggtatgctgc
    21781 attaatcatt tctttaattc agcataagtt gttatggcaa aatcagcact gttcacggtg
    21841 cgtaataatg agtcctgccc aaagtgcggg gctgaactgg ttattcgatc cgggaaacac
    21901 ggtccgtttc ttggatgctc acagtatccg gcgtgtgact acgtccgtct tctgaaatct
    21961 tcagcggatg gacatattgt caaagttctg gaggggcagg tttgccctgc atgtggcgca
    22021 aatctggtat tacgccaggg acgctttggt atgtttattg gttgcagtaa ctaccctgaa
    22081 tgcgaacata ccgaacttat cgataaaccg gacgaaacag caattacatg cccccaatgt
    22141 cggacgggcc atctggtcca gcgccgctcc cgttatggca aaacatttca ctcctgtgat
    22201 cgctacccgg agtgtcaatt tgccattaac ttcaaaccaa tagctggaga atgccctgag
    22261 tgtcattatc cgctactcat cgaaaagaaa accgcgcagg gtgtaaaaca cttttgtgcc
    22321 agtaaacaat gtggaaagcc ggtttcggcg gaataataac gtgaataata acctgcaagg
    22381 agacgctatc gcagctgcga tagatgtcct caatgaagaa cgtgtcatcg cctatccaac
    22441 ggaagccgtt ttcggtgttg ggtgcgatcc tgatagcgaa acagcagtga tgagactgtt
    22501 ggagttaaaa cagcgtccgg ttgataaggg gctgatttta atcgcggcaa attacgagca
    22561 gcttaaaccc tatattgatg acaccatgct gactgatgcg cagcgtgaaa ccattttttc
    22621 ccgctggcca ggtcctgtca cctttgtctt tcccgcgcct gcgacaacac cgcgctggtt
    22681 gacgggccgc tttgattcgc ttgctgtacg agtcaccgac catccgttgg tggttgcttt
    22741 gtgccaggct tatggtaaac cgctggtttc taccagtgcc aacttgagtg gattgccacc
    22801 ttgtcgaaca gtagacgaag ttcgcgcaca atttggcgcg gcgttcccgg ttgtgcctgg
    22861 tgaaacgggg gggcgtttaa atccttcaga aatccgcgat gccctgacgg gtgaactgtt
    22921 tcgacagggg taacataatg gaaacctatg ctgtttttgg taatccgata gcccacagca
    22981 aatcgccatt cattcatcag caatttgctc agcaactgaa tattgaacat ccctatgggc
    23041 gcgtgttggc acccatcaat gatttcatca acacactgaa cgctttcttt cgtgctggtg
    23101 gtaaaggtgc gaatgtgacg gtgcctttta aagaagaggc ttttgccaga gcggatgagc
    23161 ttactgaacg ggcagcgttg gctggtgcag ttaataccct caagcggtta gaagatggac
    23221 gcctgctggg tgacaatacc gatggtgtag gcttgttaag cgatctggaa cgtctgtctt
    23281 ttatccgccc tggtttacgt attctgctta tcggcgctgg tggagcatct cgcggcgtac
    23341 tactgccact tctttcccta gactgtgcgg tgacaatcac taatcggacg gtatcccgcg
    23401 cggaagagtt ggctaaattg tttgcgcaca ctggcagtat tcaggcgttg ggtatggacg
    23461 aactggaagg tcatgagttt gatctcatta ttaatgcaac atccagtggc atcagtggtg
    23521 atattccggc gatcccgtca tcgctcattc atccaggcat ttattgctat gacatgttct
    23581 atcagaaagg aaaaactcct tttctggcat ggtgtgagca gcgaggctca aagcgtaatg
    23641 ctgatggttt aggaatgctg gtggcacagg cggctcatgc cttccttctt tggcatggcg
    23701 ttctgcctga tgtagaaccg gtcatcaaac tattgcaaca ggaattgtca gcgtgaatca
    23761 ggccatccag tttccggata gggaagagtg ggacgagaat aaaagatgtg tatgttttcc
    23821 tgctctcgtg aatggtatgc aactgacatg tgcgatctct ggcgagagtc tggcgtatcg
    23881 ctttactgga ggtacgccag aacagtggtt agcgagtttt cgtcagcatc gctgggacct
    23941 ggaagaagaa gcggaaaact taattcagga acaaagtgaa gatgatcaag gttgggtctg
    24001 gttaccttga tccagatatt cgtccttcca tttcacgtag ttatttgcgg aatagcgtaa
    24061 cccagccttc tcttcatcac ttaacgggcg gatctgtttg acgggactac cgagatacag
    24121 atatccgctc tccagccgtt tattttgtgg gaccaggcta cccgcaccaa tcatcacatc
    24181 atcttctact attgcgccat caagtaaaat tgagcccatc ccaaccaaaa ctcgattgcc
    24241 aatggtgcag ccgtggagca tcaccttgtg accaacagtg acatcttcgc caatggttaa
    24301 tgggttgcca tctgggttgt acgaggattt atgagtgaca tgcaacatac tgccatcctg
    24361 gatattggtg cgtgctccga tctgtacata atgtacatct ccacgaatca caacgagcgg
    24421 ccagatcccc acatcatcag ccagacgaac gtcaccaatc acgacgctgc tatcgtcgat
    24481 cattacgcgc tggccgattt gtggaaaaag atcgcggtat gggcgtaaaa catcagacat
    24541 actcacctca acaataaatg atttcctaat gactttgggt gcattattag ccttgtgcaa
    24601 gtcttttagt atgcaaaaaa gcaccgtttt gtgtgcgaat gcagcaaaaa gggtgaaaaa
    24661 acaacaaaca gaaaaaaaga tcaaaaaagt acttgtgcaa aaaattggga tccctataat
    24721 gcgcctccgt tgagacgaca acgtgaaaca cttcacagga tggtcggaac aacgaagaga
    24781 aaaaattctg aaattcaggg ttgactctga aagaggaaag cgtaatatac gccacctcgc
    24841 gacagtgagc tgaaagccgc gtcgcaactg ctctttaaca atttatcaga caatctgtgt
    24901 gggcactcga agatacggat tcttaacgtc gcaagacgaa aaatgaatac caagtctcaa
    24961 gagtgaacac gtaattcatt acgaagttta attctttgag catcaaactt ttaaattgaa
    25021 gagtttgatc atggctcaga ttgaacgctg gcggcaggcc taacacatgc aagtcgaacg
    25081 gtaacaggaa gcagcttgct gcttcgctga cgagtggcgg acgggtgagt aatgtctggg
    25141 aaactgcctg atggaggggg ataactactg gaaacggtag ctaataccgc ataa
//
LOCUS       DAZPEP010000044        25106 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZPEP010000044 DAZPEP010000000
VERSION     DAZPEP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.044"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            196..384
                     /gene="bcsR"
                     /locus_tag="ACHUT9_001834"
     CDS             196..384
                     /gene="bcsR"
                     /locus_tag="ACHUT9_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFR1348326.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHUT9_001835"
     CDS             396..1148
                     /gene="bcsQ"
                     /locus_tag="ACHUT9_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFR1348327.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1145..1309
                     /locus_tag="ACHUT9_001836"
     CDS             1145..1309
                     /locus_tag="ACHUT9_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HFR1348328.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHUT9_001837"
                     /pseudo
     CDS             1316..3763
                     /gene="bcsA"
                     /locus_tag="ACHUT9_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHUT9_001838"
     CDS             3774..6113
                     /gene="bcsB"
                     /locus_tag="ACHUT9_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFR1348329.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVLRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHUT9_001839"
     CDS             6120..7226
                     /gene="bcsZ"
                     /locus_tag="ACHUT9_001839"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFR1348330.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHUT9_001840"
                     /pseudo
     CDS             7208..10700
                     /gene="bcsC"
                     /locus_tag="ACHUT9_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
     gene            10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHUT9_001841"
     CDS             10782..12770
                     /gene="hmsP"
                     /locus_tag="ACHUT9_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFR1348331.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12953..14239
                     /gene="dctA"
                     /locus_tag="ACHUT9_001842"
     CDS             12953..14239
                     /gene="dctA"
                     /locus_tag="ACHUT9_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFR1348332.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14459..15955
                     /locus_tag="ACHUT9_001843"
     CDS             14459..15955
                     /locus_tag="ACHUT9_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1348333.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHUT9_001844"
     CDS             complement(16051..16980)
                     /gene="kdgK"
                     /locus_tag="ACHUT9_001844"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFR1348334.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHUT9_001845"
     CDS             17212..17979
                     /gene="pdeH"
                     /locus_tag="ACHUT9_001845"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFR1348335.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18049..20109
                     /locus_tag="ACHUT9_001846"
     CDS             18049..20109
                     /locus_tag="ACHUT9_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1348336.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20291..21613)
                     /locus_tag="ACHUT9_001847"
     CDS             complement(20291..21613)
                     /locus_tag="ACHUT9_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1348337.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASIAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHUT9_001848"
     CDS             complement(22024..23037)
                     /gene="yhjD"
                     /locus_tag="ACHUT9_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFR1348338.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23086..23985)
                     /locus_tag="ACHUT9_001849"
     CDS             complement(23086..23985)
                     /locus_tag="ACHUT9_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1348339.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24505..>25106
                     /locus_tag="ACHUT9_001850"
     CDS             24505..>25106
                     /locus_tag="ACHUT9_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFR1348340.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6364 c         6991 g         5856 t
ORIGIN      
        1 atttagcttg tgaatcaatt tagaaggtca caagacgtgt gattttagtc ttttcgtttt
       61 taactttttc tgttgcgtaa cggaaagtca aaaagtgagc aaattcccgt ctcgccgtaa
      121 acaataaacc ggcgaaatgc tcacggttag aattgtttca tgaacggtac ggttatttca
      181 tagggatcaa gcaaaatgaa taacaatgaa ccagatactc tgcctgatcc cgcgataggc
      241 tatatcttcc agaatgatat tgtggcgtta aagcaggcat tttcactgcc tgatattgat
      301 tatgccgata tttcccaacg cgaacagttg gccgcggcat taaaacgctg gccgttgctg
      361 gcagagtttg cgcaacaaaa gtaggggatt ggtgaatggc cgtactggga ttgcaggggg
      421 tgcggggagg cgtggggaca acaaccatca ccgccgcatt agcctggtca ttacaaatgt
      481 tgggagaaaa tgtcctggtg gtcgatgcct gcccggacaa cttgttgcgc ctgtcattta
      541 atgttgattt tacccaccgt cagggctggg ccagagcgat gctggatggc caggactggc
      601 gtgacgctgg gttgcgctac acctcgcagc tcgatttgct gccttttggt cagttatcca
      661 ttgaagaaca agaaaatcca cagcactggc aaacccggct gagcgatatt tgctccggct
      721 tacagcaact aaaagccagc gggcgttacc agtggatttt aatcgactta ccgcgtgatg
      781 cctcgcagat aacccaccag ctgctgagtt tgtgcgatca ctcgctggca atcgtcaatg
      841 tggatgccaa ctgccatatc cgactgcatc agcaagcgct gccggatggc gcacatattt
      901 tgattaatga cttccgtatt ggcagtcagg ttcaggacga tatttaccag ctttggttgc
      961 aaagccagcg ccgattactg ccgatgctca ttcatcgtga tgaagcgatg gctgaatgcc
     1021 tggcggctaa gcaaccagta ggtgaatatc gcagtgatgc gctggcggct gaagagatac
     1081 tgacgctggc gaactggtgc ctgttgaact actccgggct gaaaacgcca gtcgggagtg
     1141 catcatgagt atcctgaccc ggtggttgct tatcccgccg gtcaacgcgc ggcttatcgg
     1201 gcgttatcgc gattatcgtc gtcacggtgc gtcggctttc agcgcgacgc tcggctgttt
     1261 ctggatgatc ctggcctgga tttttattcc gctggagcac ccgcgctagc agcgtattcg
     1321 cgcagaacat aaaaacctgt atccgcatat caacgcctcg cgtccgcgtc cgctggaccc
     1381 ggtccgttat ctcattcaaa catgctggtt actgatcggt acatcgcgca aagaaacgcc
     1441 gaaaccgcgc aggcgggcat tttcaggtct gcagaatatt cgtagacgtt accatcaata
     1501 gatgaacgag ctgcctgagc gcgttagcca taaaacacag catcttgatg agaaaaaaga
     1561 gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg tcaccttctc
     1621 gctgattctg gcgttaatct gcgttactca accgtttaac ccgctggcgc agtttatctt
     1681 cctgatgctg ctgtggggtg tagcgctgat cgtacggcgg atgccggggc gcttctcggc
     1741 gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt ggcgttacac
     1801 ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc tgctcttcgc
     1861 tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat ggccgctgaa
     1921 tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg atatctttgt
     1981 cccgacttac aacgaaaatc tcaatgtggt gaaaaatacc atttacgcct cgctgggtat
     2041 cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca gggaagagtt
     2101 tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc atgaacatgc
     2161 gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg tgtcgatttt
     2221 cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct ggttcctgaa
     2281 agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg acccgtttga
     2341 acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct atggtctggt
     2401 gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctatg cggtgattcg
     2461 tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg aagatgcgca
     2521 tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta ttccgcaggc
     2581 ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc gctgggcgcg
     2641 cgggatggta caaatcttcc gtctcgataa cccgctcacc ggtaaaggac tgaagtttgc
     2701 tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc cacggctgat
     2761 cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct atgcgccagc
     2821 gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga ccaactcgaa
     2881 gatccagggc aaatatcgcc actctttttg gagtgaaatc tacgaaacgg tgctggcgtg
     2941 gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca aatttaacgt
     3001 caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct cgcggcccta
     3061 catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc gctacttcta
     3121 tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt tctacaacct
     3181 gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac gccgatcgca
     3241 ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc tcttctcgtg
     3301 taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc aggcgcagat
     3361 tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat acgtcttccc
     3421 gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc cgctcaccac
     3481 ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat gggcgctctg
     3541 gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc tgaagctcgg
     3601 cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg gcatattccg
     3661 tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccacc cggagcggag
     3721 cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac gcgatgaaaa
     3781 gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc tctttcatga
     3841 cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta gccgcccaga
     3901 cggaacaaaa tccgcaggtg gggcaagtga tgccgggcgt gcagggcgct gatgcgccag
     3961 tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa attgcgccgc
     4021 cgccgggcag catggtgcta cgtggcatta acccgaacgg cagcattgag tttggtatgc
     4081 gcagcgatga agtggtgacg aaggcgatgc tcaacctcga atacacgcca tcgccatcgt
     4141 tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg ggcgtgctgc
     4201 cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt aacccactgt
     4261 ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag gacgtgtgcg
     4321 aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga ctggatctga
     4381 cctatcagac cctgaatgtg aagagtgacc tgtcacactt cccggtgcca ttctttgacc
     4441 cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg gatgttgggc
     4501 tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc tggcgtgggc
     4561 agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc tttgcaacca
     4621 acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg gtgattgaga
     4681 tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt cgtgacgaca
     4741 aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc cgtggtgaaa
     4801 gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat gcgccgaact
     4861 gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa gaacagttac
     4921 aatccagcgg tcttgagcca gcagcgatta acgtttcgct aaacctgccg ccggatctct
     4981 acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc atgccgccgg
     5041 tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa tccttcaacc
     5101 tgagcagtaa acaggaggcg aaccgcctgc tgctgcggat tccggtatta caaggtttgc
     5161 tggatggcaa aacggatgtc tctattccgg cgctgaaact gggcgcgacc aaccagctgc
     5221 gcttcgactt tgagtatatg aacccgatgc cgggcggttc ggtggataac tgtattacct
     5281 tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac ttctcgaagt
     5341 attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc ttcccgttca
     5401 gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaaacgcct aacgaagcac
     5461 agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc ttcccggcga
     5521 ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc gacatcatga
     5581 tcatcggtgg tatcccggac aaattaaaag acgataagca gatcgacctg ttggtgcagg
     5641 cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc attgtgccgg
     5701 acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt gcgatggcgg
     5761 cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg ctgctggcag
     5821 atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc aaacgcgcca
     5881 ccatgttcgg ttcggtcgcg gtgctccgcg agtccggtat caacagccta cgtgttggcg
     5941 acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg ctggcaaacc
     6001 atccgattct gctggcggtg ctggcggcaa tcagtgtgat attgctggca tgggtactgt
     6061 ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag taattgaaga
     6121 tgaatgtgtt gcgtagtgga atcgtgacga tgctgctgtt ggctgccttt agtgttcagg
     6181 cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt caggaagggc
     6241 gcgtcatcgc ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa agttacggta
     6301 tgttctttgc cctggcggct aacgaccgtg tagctttcga taatattctc gactggacgc
     6361 agaacaatct cgctcagggc tctttaaaag aacgtttgcc cgcctggctg tggggcaaga
     6421 aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt gatgtctgga
     6481 tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat accgacatcg
     6541 gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct gggctgggtt
     6601 ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt tttaacccca
     6661 gctacctgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg tggaccacgc
     6721 tgcgcgaaac caatcaacgt ttattgctgg aaaccgcccc gaaaggtttt tcgccagact
     6781 gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca ttgatcagca
     6841 gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc gatccgcaaa
     6901 aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa aacggttatc
     6961 cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg atcggttttt
     7021 ctgccgccat gctgcccttt ttacaaaacc gcgatgcgca ggccgttcag cgccagcgcg
     7081 tggccgataa ctttcccggc agcgatgcct attacaacta tgtgctgacc ctgtttggac
     7141 aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta cctgactggg
     7201 gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc tggccgtcat
     7261 gccgatggtc gaggcagcac caaccgctca gcaacagttg ctggagcaag ttcggttagg
     7321 cgaagcgacc catcgtgaag atctggtgca acagtcgtta tatcggctgg aacttattga
     7381 tccgaataac ccggacgtcg ttgccgcccg tttccgttct ttgttacgtc agggcgatat
     7441 tgatggcgcg caaaaacagc tcgatcggct gtcgcagtta gcgccgagtt caaatgcgta
     7501 taaatcgtcg cggactacga tgctactttc cacgccggat ggtcgtcagg cactgcaaca
     7561 ggcacgattg caggcgacga ccggtcatgc agaagaagct gtggcgagtt acaacaaact
     7621 attcaacggt gcgccgccgg aaggtgacat tgctgtcgag tactggagta cggtggcgaa
     7681 aattccggct cgccgtggcg aagcgattaa tcagttaaaa cgcatcaatg cggatgcacc
     7741 gggcaatacg ggcctgcaaa acaatctggc gctattgctg tttagtagcg atcgccgtga
     7801 cgaaggtttt gccgtcctgg aacagatggc aaaatcgaac gccgggcgcg aaggggcctc
     7861 taaaatctgg tacgggcaga ttaaagacat gcccgtcagt gatgccagtg tgtcggcgct
     7921 gaaaaaatat ctctcgatct ttagtgatgg cgatagcgtg gcggctgcgc aatcgcaact
     7981 ggcagaacag caaaaacagc tggccgatcc tgctttccgc gctcgtgcgc aaggtttagc
     8041 ggcggtggac tctggtatgg cgggtaaagc cattcccgaa ctacaacagg cggtgcgggc
     8101 gaacccgaaa gacagtgaag ctctgggggc gctgggccag gcgtattctc agaaaggcga
     8161 tcgcgccaat gcagtggcga atctggaaaa agccctcgca ctggacccgc acagcagcaa
     8221 caacgacaaa tggaacagtc tgctgaaagt aaaccgctac tggctggcga tccagcaggg
     8281 cgatgctgcg ctgaaagcca ataatcctga ccgggcagaa cgcctgttcc agcaggcgcg
     8341 taatgtcgat aacaccgaca gttatgcagt gctggggctg ggcgatgtgg cgatggcgcg
     8401 aaaagattat cccgccgccg aacgttatta tcagcagacc ttgcgtatgg acagcggcaa
     8461 cactaacgcc gtgcgcgggc tggcaaatat ttaccgccag caatcgccag aaaaagctga
     8521 agcgtttatc gcctcgctct ctgccagtca gcggcgtagc attgatgata tcgaacgcag
     8581 cctgcaaaac gaccgtctgg cacagcaggc agaggcactg gaaaaccagg gcaaatgggc
     8641 gcaggcggca gcacttcagc ggcaacgact ggcgctggac cccggcagcg tatggattac
     8701 ttaccgactt tcgcaggatc tctggcaggc cggacaacgc agccaggccg atacgttaat
     8761 gcgcaatctg gcgcagcaga agtcgaacga cccagagcag gtttacgctt acgggctgta
     8821 cctacgctta cgggctgtac ctctctggtc atgaccagga cagagcggcg ctggcgcata
     8881 tcaacagcct gccgcgtgcg cagtggaaca gcaatattca ggagctggtt aatcgactgc
     8941 aaagcgatca ggtgctggaa accgctaacc gcctgcgaga aagcggcaaa gaggcagaag
     9001 cggaagcgat gctgcgccag caaccacctt ccacgcgtat tgacctcacg ctggctgact
     9061 gggcgcaaca acgacgtgat tacaccgccg cccgcgctgc atatcagaat gtcctgacgc
     9121 gggagccagc taacgccgac gccattcttg gtctgacgga agtggatatt gctgccggtg
     9181 acaaagcggc ggcacgtagc cagctggcga aactgcccgc taccgataac gcctcgctga
     9241 acacacagcg gcgcgtggcg ctggcacagg cgcagcttgg cgataccgca gcagcgcagc
     9301 ggacgtttaa taagttgatc ccgcaggcaa aatctcagcc accgtcgatg gaaagcgcga
     9361 tggtgctgcg tgatggtgcg aagtttgaag cgcaggcggg cgatccaacg caggcgctgg
     9421 aaacctacaa agacgccatg gtcgcatccg gtgtgactac gacgcgtccg caggataacg
     9481 acacctttac ccgactgacc cgtaacgacg agaaagatga ctggctgaaa cgtggcgtgc
     9541 gcagcgatgc ggcggacctc tatcgccagc aggatcttaa cgtcaccctt gagcacgatt
     9601 actggggttc gagcggcacc ggtggttact ccgatctgaa agcgcacact accatgttgc
     9661 aggtggatgc gccgtattct gacgggcgga tgttctttcg cagtgatttc gtcaatatga
     9721 acgtcggcag tttctccact aatgccgatg gcaaatggga tgacaactgg ggcacctgta
     9781 cattacagga ctgtagcggc aaccgcagcc agtcggattc cggtgccagc gtggcggtcg
     9841 gctggcgaaa tgacgtctgg agctgggata tcggtaccac gccgatgggc ttcaacgtgg
     9901 tggatgtggt cggcggcatc agttacagcg atgatatcgg gccgctgggt tacaccgtta
     9961 acgcccaccg tcggcccatc tccagttctt tgctggcctt tggtgggcaa aaagactccc
    10021 cgagcaatac cgggaaaaaa tggggtggcg tacgtgccga cggtgtgggg ctaagtctga
    10081 gctacgataa aggtgaagca aacggcgtct gggcatcgct tagtggcgac cagttaaccg
    10141 gtaaaaatgt cgaagataac tggcgcgtgc gctggatgac gggctattac tataaggtca
    10201 ttaaccagaa caatcgccgc gtcacaatcg gcctgaacaa catgatctgg cattacgaca
    10261 aagatctgag tggctactca ctcggtcagg gcggttacta cagtccgcag gaatacctgt
    10321 cgtttgccat accggtgatg tggcgggagc gcacggaaaa ctggtcgtgg gagctggggg
    10381 cgtctggctc gtggtcgcat tcacgcacca aaaccatgcc gcgttatccg ctgatgaatc
    10441 tgatcccgac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    10501 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    10561 ttggcacggc aattgatatc cagcaggcga aagattacgc acccagccat ttcctgctct
    10621 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    10681 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    10741 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    10801 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    10861 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    10921 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    10981 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11041 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgcccggtgc
    11101 cgataatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtttactcgc
    11161 tcgaacgtcc ggcaaacccg cagccaattg cctatctggt actacaggcg gattccttcc
    11221 gtatgtataa gttcgtgatg agcaccctct caacgttagt gaccatttac ttacttttgt
    11281 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    11341 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    11401 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    11461 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    11521 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    11581 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    11641 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    11701 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    11761 gtgtacagga accgtggcac gcaatcacct taggtcagca agtgctcact atcatgagcg
    11821 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    11881 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    11941 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagccgccc
    12001 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcta
    12061 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12121 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12181 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12241 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    12301 aactgatgca cccaaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    12361 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    12421 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    12481 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    12541 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    12601 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    12661 cacaacgcga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    12721 acccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    12781 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    12841 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaaggc
    12901 cgcaggtacc ccataacctt acaagacctg tggttttact aaaggacacc ctatgaaaac
    12961 ctctctgttt aaaagccttt actttcaggt cctgacagcg atagccattg gtattctcct
    13021 tggccatttc tatcctgaaa taggcgagca aatgaaaccg cttggcgacg gcttcgttaa
    13081 gctcattaag atgatcatcg ctcctgtcat cttttgtacc gtcgtaacgg gcattgcggg
    13141 catggaaagc atgaaggcgg tcggtcgtac cggcgcagtc gcactgcttt actttgaaat
    13201 tgtcagtacc atcgcgctga ttattggtct tatcatcgtt aacgtcgtgc agcctggtgc
    13261 cggaatgaac gtcgatccgg caacgcttga tgcgaaagcg gtagcggttt acgccgatca
    13321 ggcgaaagac cagggcattg tcgccttcat tatggatgtc atcccggcga gcgtcattgg
    13381 cgcatttgcc agcggtaaca ttctgcaggt gctgctgttt gccgtactgt ttggttttgc
    13441 gctccaccgt ctgggcagca aaggccaact gatttttaac gtcatcgaaa gtttctcgca
    13501 ggtcatcttc ggcatcatca atatgatcat gcgtctggca cctattggtg cgttcggggc
    13561 aatggcgttt accatcggta aatacggcgt cggcacactg gtgcaactgg ggcagctgat
    13621 tatctgtttc tatattacct gtatcctgtt tgtggtgcta gtattgggtt caatcgctaa
    13681 agcgactggt ttcagtatct tcaaatttat ccgctacatc cgtgaagaac tgctgattgt
    13741 actggggact tcatcttccg agtcggcgct gccgcgtatg ctcgacaaga tggagaaact
    13801 cggctgccgc aaatcggtgg tggggctggt catcccgaca ggctactcgt ttaaccttga
    13861 tggcacatcg atatacctga caatggcggc ggtgtttatc gcccaggcca ctaacagtca
    13921 gatggatatc gtccaccaaa tcacgctgtt aatcgtgttg ctgctttctt ctaaaggggc
    13981 ggcaggggta acgggtagtg gctttatcgt gctggcggcg acgctctctg cggtgggcca
    14041 tttgccggta gcgggtctgg cgctgatcct cggtatcgac cgctttatgt cagaagctcg
    14101 tgcgctgact aacctggtcg gtaacggcgt agcgaccatt gtcgttgcta agtgggtgaa
    14161 agaactggac cacaaaaaac tggacgatgt gctgaataat cgtgcgccgg atggcaaaac
    14221 gcacgaatta tcctcttaat ctcaccactt atgcccgcac tcccttcccc gagtgcgggt
    14281 gtttgcgcat aattgacccc tgtgcctgaa cttaccacct gggtgagttc aggtttattt
    14341 cacttcttcc gtgacatttt catgttcttg cggtctaaca cgaagtgttt ttaacgtcat
    14401 ttagactgac tgacaacggg cagtttttta ttaccaggat agttgatcag gggttcacat
    14461 gcagggcaca aaaattcgac ttttagcggg cggtttgctg atgatggcca ctgctggcta
    14521 tgtgcaggca gatgcgctcc agcctgatcc agcatggcaa caggggacgc tttccaacgg
    14581 tttacagtgg caagtgctga ccacccccca gcgtcccagc gatcgtgttg aaattcgcct
    14641 gctggttaat atcggttcgc tcgccgaaag tacacaacag agcggttaca gtcacgccat
    14701 ccctcgtatt gcgctaacgc aaagcggtgg ccttgacgca gcacaggcgc gttcattgtg
    14761 gcagcagggg atcgacccta aacgcccgat gccgccggta attgtctctt atgacaccac
    14821 gctgtttaat ctgagtttgc ccaataaccg taacgatttg ctgaaagaag cgctctctta
    14881 tctggcaaat gccactggca aattgaccat cacaccagaa accatcaacc acgcgctgca
    14941 aagtcaggac atggtggcaa cctggcctgc cgatactaaa gagggctggt ggcgctatcg
    15001 tctgaaaggg tcaaccttgt taggtcacga tcctgccgat ccgctgaaac aacccgttga
    15061 agcggaaaaa attaaagatt tctatcagaa atggtacacc ccggatgcaa tgacgctact
    15121 ggtggtggga aacgtggatg cgcgctcggt tgtcgaccaa atcaacaaaa cgtttggcga
    15181 actgaaaggc aaacgtgaaa cgccagctcc ggtgccgacg ctttctccgc tgcgtgcgga
    15241 agcggtgagt attatgactg acgcggtgcg tcaggaccgg ttatctatca tgtgggatac
    15301 gccgtggcag ccgattcgtg aatcagccgc actgctgcgc tactggcgtg cggacctggc
    15361 ccgcgaggcg ctgttctggc atgttcagca agcgttaagc gccagtaaca gcaaagacat
    15421 cggtcttgga tttgactgcc gtgtgctgta tctgcgtgcg cagtgtgcca tcaacatcga
    15481 atcaccaaac gacaagctga acagcaacct taatctggtg gcgcgtgaac tggcgaaggt
    15541 tcgcgataaa ggtctgccgg aagaagagtt caatgcgtta gtggcgcaaa agaaactgga
    15601 gctgcagaaa ctgtttgccg cctatgcgcg ggctgatacc gatattctga tgggtcagcg
    15661 gatgcgttcg ctgcaaaatc aggtcgtcga tatcgcgccg gagcagtatc agaaactgcg
    15721 gcaggatttc cttaacagcc tgacggtgga gatgttaaat caggatctgc gtcagcagtt
    15781 gtcgaatgat atggcgttaa tactgctgca gccgaaaggc gagccggaat ttaacatgaa
    15841 agcgttgcag gcggcctggg atcaaatcat ggccccatcg actgctgctg ccgccacctc
    15901 tgtcgccacg gatgacgtac atcctgaagt gacggatatt ccacctgcac agtaatgcct
    15961 gatggtgatg agttagcaca accatcaggt atttatggat gagctggata gaggggtaac
    16021 actttatcca gccttttgca tatgctgcgt ttacgctggc atcgcctcac gcgggataat
    16081 cgcgccgcga tactgaataa cggtacttgc ggtcaggtgc ccgcgtttcg ccgcgttttc
    16141 cgcactgccg cccgtcaaac ataccgccag ataaccggcg ctgaaagagt cgccagctgc
    16201 ggtggtatcg atcacttttt ctttcggcag tttcaccgcc ggaacatcca ctaacccttc
    16261 gccagcaatg gacaccaggc aagaatccgc cccgcgtttc accaccactt ctttcacgcc
    16321 cgcgttatgg gtgcgcgcaa tgacgtcttc caccggctgt tgaccccaca gcgcgtcttc
    16381 atcgtccagc gtcaggaagg cgatatccgt gcattccagc atttgttggt acacctgctg
    16441 tgtctcttct ttgctggccc acaggcgcgg acgatagtta ttgtcgaaaa tcacttttcc
    16501 gccgttggcg cggcattcgc gcagcaggga aagcagcttt tcgcggctgg tcgggcttaa
    16561 gatcgccagg ctaatcccgc tcaggtagag ataatcgaaa ttcgccagct cttcgcaaat
    16621 cgccgcagac tgctcactct ccagccagaa tttggcggcg gcttcgttcc gccagtagta
    16681 gaacgtacgc tcgccggtgc tgtcggtttc aatgtagtaa aggcccggca gacggttttc
    16741 catccgttgg gtcagggaag tatcaacgtt ctcgccgtgc caggcgtcca gcatttgctg
    16801 gctaaaactg tccgttccca gcgccgttac gtaatgaacg gttaatgccg caggatcgac
    16861 ctgacgggcg atatagacgg aagtgttcag ggtatcgccg ccgaaaccgc gcttaacgtc
    16921 cgcgcctttc tcggaaagct caatcatgca ttcgccaatc acggcaatct ttttggacat
    16981 agtcgtgaac ctgatctgta aaaaattagc gttagtttgc gctgtggtgg tttgctggtc
    17041 aaccatatta aaacagtgtt ccattatttt tttgagccag aacgtattcc tgaaagattc
    17101 cgttgtggag tgaggaaatt taccccggcc aggcggtaaa gttctgccct tgcgcgccga
    17161 taatctttgt cgagtccggg cagcatcact tttaaacaca ggacatcttt gatgataagg
    17221 caggttatcc agcgaataag caaccctgaa gcaagcatcg agagcttgca ggaacggcgt
    17281 ttttggttgc agtgtgagcg tgcttacacc tggcagccga tctatcaaac atgcggacgg
    17341 ttaatggccg tggagctatt aacggtggtc acgcatccct tgaacccttc gcaacgcctg
    17401 ccgccggatc gctattttac tgaaatcacc gtcagccatc ggatggaggt tgtgaaagag
    17461 cagattgatt tgctggcgca aaaagccgac ttctttgtag agcacgacct gctggcatcg
    17521 gtcaatattg atggccctac gctcatcgcc ctgcgtcagc aaccaaaaat cctgcgccaa
    17581 attgagcgtc ttccctggct gcgtttcgaa ctggtggaac acatccgcct gccgaaagat
    17641 tcaacctttg cctcgatgtg tgaatttggc ccgctgtggc tggatgattt tggcaccggg
    17701 atggcaaatt tctctgcgct aagtgaagtg cgttatgact acatcaaaat cgcgcgagag
    17761 ctgttcgtga tgctgcgtca gtcgccggaa ggccgcacac tcttttctca gcttttacat
    17821 ctaatgaatc gctattgtcg cggggtgatt gtcgagggcg tagaaacacc ggaagagtgg
    17881 cgtgatgttc agaactcgcc cgcattcgcc gcacaaggct ggtttctttc acgcccggca
    17941 ccgatggaaa cgctgaatac ggcggttctg gcgctataag ctgcctcatt ttccgctggg
    18001 ctggactatc tttaggactg gcacaggaag aatatgagga agcgaacgat gagcaaggca
    18061 ggcaaaataa ccgctgcgat ttcaggggct ttcttgttgt tgattgtcgt ggcgatcatt
    18121 ttgattgcaa cttttgactg gaatcgactc aaaccgacca tcaaccagaa agtctctgcg
    18181 gagttgaatc gtccgttcgc tatccgtggc gatctgggcg tggtgtggga gcggcaaaaa
    18241 caagaaactg gctggcgcag ctgggtgccg tggcctcatg tacatgcaga agacatcatt
    18301 cttggtaatc caccggatat tcccgaagtc acgatggtgc atttgccgcg agtagaggca
    18361 acgctggcac cgctggcgct gctgaccaaa acggtctggc tgccgtggat caagctcgaa
    18421 aagcccgacg cgcgcctgat tcgcctctcc gaaaagaaca ataactggac gtttaatctt
    18481 gccaacgatg acaacaaaga cgcgaatgca aagccgtcgg catggtcgtt tcggctggat
    18541 aatattcttt tcgatcaagg gcggatcgcc attgatgaca aagtaagcaa agcggatctg
    18601 gaaatttttg ttgatccgtt aggcaagccg ctgccgttca gcgaagttac tggatcgaaa
    18661 ggtaaagcgg ataaagaaaa ggtgggcgat tacgtttttg gcctgaaggc gcagggacgt
    18721 tataacggtg aaccgctcac gggtacggga aaaataggcg gtatgctggc gctgcgtggc
    18781 gaagggacgc cgtttccggt acaggctgat ttccgttcag gtaatacccg tgttgctttt
    18841 gatggcgtcg tgaatgaccc aatgaagatg ggcggtgtcg atttacggct taaattttct
    18901 ggcgattcac tgggtgatct ctatgaactg acgggcgttc tgctgcccga taccccgccg
    18961 tttgaaacgg atggtcggct ggtagcgaaa atcgacactg aaaaatcgtc ggtctttgat
    19021 tatcgcggtt ttaatgggcg aattggcgat agcgatatcc acggttctct ggtctacacc
    19081 accggaaagc cacgaccaaa actggaaggt gatgtcgagt cgcggcaatt gcggctggcg
    19141 gacctgggac cgttgattgg cgttgattcc gggaaagggg cagaaaagtc gaaacggtct
    19201 gaacagaaga agggcgaaaa aagcgttcag cctgcgggca aagtgctgcc ttatgaccgc
    19261 ttcgaaaccg ataaatggga cgttatggat gccgatgttc gcttcaaagg gcggcgcatt
    19321 gagcatggca gtagcctgcc gattagcgat ctttctactc atatcatcct caaaaatgct
    19381 gacctgcgcc tgcaaccgct gaaatttggc atggcgggcg gcagcattgc ggcgaatatt
    19441 catctggaag gcgataaaaa gccgatgcag gggcgggcag atattcaggc tcgtcgactg
    19501 aaactgaaag aactgatgcc cgatgtggaa ctgatgcaga agacgctggg ggaaatgaac
    19561 ggtgacgcgg aactacgcgg tagcggtaac tcggtggcgg cgcttttagg caacagtaac
    19621 ggcaacctga aactgttgat gaatgacggg ctggtgagcc gcaacctgat ggagattgtt
    19681 gggctgaatg tcggcaacta cattgtcggt gcgatatttg gtgacgatga ggtgcgggtg
    19741 aactgcgcgg cggcgaatct gaatattgcc aacggcgtgg cgcgcccgca gatttttgct
    19801 ttcgatactg agaacgcgtt gattaatgtt accggcacgg caagttttgc ttcagaacag
    19861 ctggatttga ctattgatcc ggagagtaaa gggattcgga ttatcacact gcgttcgccg
    19921 ctgtatgtgc gtgggacgtt taaaaatccg caggctgggg tgaaagccgg accgctgatt
    19981 gcccgtggtg ctgttgctgc ggcactggca acgctggtaa caccgacggc ggcgttactg
    20041 gcactgatct caccttccga aggggaggct aatcagtgtc ggacgatatt gtcgcagatg
    20101 aagaagtgat gataattgcc ggatgtctga catccggcaa ttaaacagat ttagattttt
    20161 ggcaaagtgt gctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaatc
    20221 cttgtgaatt caatatattg tatggaaatt cagtcctgat aagcgtagcg catcaggctt
    20281 ttcacactta ttacaacgac tgatgtcgcg tctcatgggt cagcagcagg gcgattaacg
    20341 tcaagccagc catcgccgcc agatataacc ccaccgcacc taacccgtag ttagtctgca
    20401 accaggctgc gatatacggt gcaatggaag ccccgagaat cgacgctacg ttgtaagaga
    20461 atgatgctcc ggtgtaacgc acttctgttg ggaacagctc tggtaacagc gcgcccatcg
    20521 gcccgaaggt cagccccatc agacttaacc ccagcagcag gaaggcaaaa accagaatcg
    20581 ggttgccaga accgagcagc gggttaaagg cgaacagcgc gaacaggatg atcagcgtgg
    20641 tgataattac catgctttta cggcgaccaa aggcatcagc cagtaatccg gcgactggca
    20701 ccatcacgcc aaaaccaata actgccatca tcagcatcca caacacttcg ttacgcggca
    20761 ggccaagccc aactggcgcg gcggcggtac tgaaggtcat agagtagacc gtcatgatgt
    20821 aaaacagcgt ataggttgcc agcatgatga acgtacccag tacggttacg cgaacatgtt
    20881 tggtcagcag cgtacccagc gggatcttca cctgtttttt cgctttagcg actttctcaa
    20941 acaccggcga ctcatgcagc gacacgcgaa catacaggcc gataatgacc agtaccgccg
    21001 agaagataaa tggcacacgc cagccccagc tcataaactg ctcgtcggtc agtagccagg
    21061 aaagcagcaa gaaagtgccg ttggcaaaga agaagccaat cggtgcgccc agctgtggga
    21121 aggagccata cagtgcacgt ttgcgcggtg gggcgttttc agtcgccagc agcgccgcgc
    21181 cgccccattc accgcctaag cccagaccct gaccaaatcg agccagcgcc agcagcagcg
    21241 gggcgaaaat accaatcgtg gcatagcccg gcagcagacc aatcaccacg gttgaaatcc
    21301 ccatcgttag caacgaggcg accagcgtcg ctttacgccc aacgcgatcg ccaaaatgac
    21361 caaaaacggc agagccaatg gggcgcgcga cgaaggcgat ggcgaaggtg gcgagcgact
    21421 gtagcgttgc tgccgttgga tcgccctgcg ggaagaagat atgcggaaac acaataacgg
    21481 ccgcagtggc gtaaatgtaa aagtcgaaga actcaatggc cgtgccaatg agagaggcga
    21541 caaggacttt attacgcgag ttgatcggcg tgtattcttg ctcgtggtcg agtgttgtgg
    21601 ctgttgcttg cataatcttt tcttattttt tggctaacga atagccatat tacgcacagc
    21661 aaaagtgata tttcaatgtg taacaaatcg cggaaatggt caaaaaccag gcaaaaaggg
    21721 gataatgttt aggccagtga tttttcttct atgaaatgct tcacataatt ttataaatag
    21781 tggataaaac tagtttcagg ttaaataaaa gttaccaact ggatttatat gctggggtta
    21841 tgagttggaa tgaaattgtt tgctcccaaa tagatggctg aagaggctct ggggaagagt
    21901 ttcaagaggt tatgtgcgca ttatagccgg atgcgatgct ggcgcatctt atccggccta
    21961 cggggagcca ggccgaataa aatcgtatgc cggataaggc gtttacgccg catccggcct
    22021 attttaaggc tgcgttttcc ctggcattcg cgggtcgtct ttatattcgg cggtggcaat
    22081 ccacgccgcg caaaacagcg tcaaacgggc gaagaagtag aaaaatgcca tcagccccag
    22141 cacggaacca aatgctgcgc cagaggggga tttcatcagc gatggcaggg tgtaggtcat
    22201 cacgatttta atcacttcaa aaccaatagc agccagaaat gttccgcgaa tcagggcttt
    22261 tttgcgcgga cggtggcgcg gtaaccgcca gaagatccag aagaaaagca gatagttggc
    22321 gaagatggaa atcgccaggc caatcaaccg ccacgtcggt ttcagccact caatgctgtt
    22381 caggtgcagg gcgctaataa tcatttgctg cgccgaaccg gcaaccgagg tgatcgaaag
    22441 cgtcacaatc agcgcaatca acaaaccaat cagcgaaata aaatcacgta gatattttac
    22501 ccagaacttc tcctgatctt gcggcgagcg ttcccagaca tcgcgcgact gggcgcgaat
    22561 cgcttcacgc aggttaccca tccagttgat gccggaataa agcgccacgg ccaggccgac
    22621 aagccctaca gtcgtacgct gctgaacggc ggtgttgatg gtgtttttca acgtggcggc
    22681 tagcgtcgga tcgctgatgt tttgcagaat tttgtcgaag atatcctgta gcaacatcgg
    22741 atgggaggcc agcacaaagc cccctgcggc aaacgacacc atcaaaatcg ggatcatcga
    22801 caaaaatgag aaataggtga tagccgcacc aaactggtta cccagccgat cgttaaagcg
    22861 ttctgtcgcg cgaatcaggt gcgcaataac cggttggcgt tggacttttt cggcagtggt
    22921 ggtgacggtt tccagcgcct ggctaacttt gccgcctttc tcgctattat ccaactgctt
    22981 aatcggctcg tgctccagat cctgggtggg acgtttgatc tcgttttcct gcgtcatccg
    23041 ctcttttgtc cttctgttgt ggtcattctt aaaaagtata gtcggtcagt ccaggtactc
    23101 tttcattacg ccagccagcc actgcataaa caggtttaca cgccgggaaa gctcccgacg
    23161 ctgcggataa accagcgaaa gggagagcgg ctcggcacgg tagcctggca acacttcaat
    23221 aagctgcccg gcacgcaggg cttcgcgcac ggcaatgcgc gggatctgaa taatccccag
    23281 accggcaaga ccggcggcga gatagttttc gctactgttt actgtcaaca tgccgccaga
    23341 cttaaaccac tggacgccat taacgctggc aacctcaaaa cctaacggat gtacacccag
    23401 gtgcggtgta taacgcacca ttgcgtgtga agtcagatca tcggggcttt gcggataacc
    23461 aaagcgcgtc agatagtgcg gactggcaca gttgaccatg gtcagtttgc cgaggggacg
    23521 ggcgataacg ccatcttccg gtaacgcgcc agtgcgtatc acgcaatcaa aaccatcatg
    23581 aagaatatct accggacggt tatggctact cagttccagc tcaattcccg gatgctgata
    23641 gagaaattcc gacagacgcg gcagtaacag gtttttcgcg attcctgacg ggatgtcgat
    23701 gcgtaattta ccgctgatac tggtggcatc ctgttgaaac agaccgtcca gttcgctgag
    23761 attactcaac acatcttttg cccgttgata ataggtcatg ccttctggcg tgagtttgac
    23821 ccgtcgcgta gtgcgctgga gaagctgggt gccaagttga tgttccagtg cctgtatctg
    23881 gcgcgaaaca cttccctttg gcaaagcaaa gaaatccgcc gcgcgggaaa aactttccag
    23941 ctccgcgact ttgatgaaca actgcattgc gtgaatttta tccatttctt tgaccgattg
    24001 ttgttcacag cgaaacagta acgcgcatat cgcattgttt attgtttttt ctaacatcta
    24061 atgccgggtt tcacaatgtt gtcatgacga atgtggcgat ttctcagcag gaaaagggcg
    24121 gaatagcttt atgagaaagg tgaatcccct taaacagagc gtatttttca gtgattccct
    24181 ttaaagtgaa tagaggacgt attggctatt tgtgcttatt tttgtctcat tgccatttgt
    24241 tattttggct tgtagtcgcg gaatttcatt tttaattgca tgcgtactaa ccaccaatat
    24301 aaggaaatcc tcaaaattgt tttaaatagt gccctcgaca tgtaatttca ggcgtttagc
    24361 tgtaactatt tatatcggta actattttct acgcgtcatc tctgtagaat ggccaccgcc
    24421 aaatggcgaa ttcttatttt ttctcgcggg taataccctt ccactactta agtcttttta
    24481 acgtattcag gtggagattc agttatgcaa atagtcatgt ttgacaggca gtcaatattt
    24541 attcatggaa tgaaaatcag tttacagcag cgtattccag gagtgagtat tcagggggcc
    24601 agtcaggcag acgagttatg gcaaaagctg gaaagttacc ctgaagcctt agttatgctc
    24661 gatggcgatc aggatggtga gttttgctac tggttgctgc aaaaaaccgt ggtgcagttt
    24721 cctgaggtta aggtgttaat cacggcgacg gattgcaata aacggtggtt acaggaagtt
    24781 attcatttta atgtgctggc cattgtgcct cgtgattcaa ccgtcgagac atttgcgttg
    24841 gcggtaaata gtgcggcgat ggggatgatg tttctgcctg gagactggcg gacgacgccg
    24901 gaaaaggaca ttaaagatct caaatcgtta agcgcccgcc agcgggagat tttaaccatg
    24961 ttagcggcgg gcgaatcaaa taaggagatc ggcagagcgc tgaatatcag taccggaacg
    25021 gtaaaagcgc atctggaatc cctgtatcgc cgtctggaag tgaaaaatcg cacccaggcg
    25081 gcaatgatgt taaatatctc ctcctg
//
LOCUS       DAZPEP010000045        24472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZPEP010000045 DAZPEP010000000
VERSION     DAZPEP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.045"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            90..806
                     /locus_tag="ACHUT9_001851"
     CDS             90..806
                     /locus_tag="ACHUT9_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1348341.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFLGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            836..1336
                     /locus_tag="ACHUT9_001852"
     CDS             836..1336
                     /locus_tag="ACHUT9_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFR1348342.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1428..4112
                     /locus_tag="ACHUT9_001853"
     CDS             1428..4112
                     /locus_tag="ACHUT9_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFR1348343.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4109..5197
                     /locus_tag="ACHUT9_001854"
     CDS             4109..5197
                     /locus_tag="ACHUT9_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFR1348344.1"
                     /translation="MRNRLIAAILGLFGTLIGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5280..6577)
                     /gene="exuT"
                     /locus_tag="ACHUT9_001855"
                     /pseudo
     CDS             complement(5280..6577)
                     /gene="exuT"
                     /locus_tag="ACHUT9_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            7060..8472
                     /gene="uxaC"
                     /locus_tag="ACHUT9_001856"
     CDS             7060..8472
                     /gene="uxaC"
                     /locus_tag="ACHUT9_001856"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFR1348345.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8487..9974
                     /gene="uxaA"
                     /locus_tag="ACHUT9_001857"
     CDS             8487..9974
                     /gene="uxaA"
                     /locus_tag="ACHUT9_001857"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFR1348346.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10057..10608
                     /locus_tag="ACHUT9_001858"
     CDS             10057..10608
                     /locus_tag="ACHUT9_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFR1348347.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10613..11857)
                     /gene="sstT"
                     /locus_tag="ACHUT9_001859"
     CDS             complement(10613..11857)
                     /gene="sstT"
                     /locus_tag="ACHUT9_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFR1348348.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12473..13438)
                     /gene="alx"
                     /locus_tag="ACHUT9_001860"
     CDS             complement(12473..13438)
                     /gene="alx"
                     /locus_tag="ACHUT9_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFR1348349.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(13721..14707)
                     /locus_tag="ACHUT9_001861"
     CDS             complement(13721..14707)
                     /locus_tag="ACHUT9_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFR1348350.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14786..15469)
                     /locus_tag="ACHUT9_001862"
     CDS             complement(14786..15469)
                     /locus_tag="ACHUT9_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFR1348351.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15546..16049)
                     /locus_tag="ACHUT9_001863"
     CDS             complement(15546..16049)
                     /locus_tag="ACHUT9_001863"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFR1348352.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            16134..17270
                     /gene="rlmG"
                     /locus_tag="ACHUT9_001864"
     CDS             16134..17270
                     /gene="rlmG"
                     /locus_tag="ACHUT9_001864"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFR1348353.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            17555..17869
                     /gene="higB"
                     /locus_tag="ACHUT9_001865"
     CDS             17555..17869
                     /gene="higB"
                     /locus_tag="ACHUT9_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFR1348354.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            17866..18282
                     /gene="higA"
                     /locus_tag="ACHUT9_001866"
     CDS             17866..18282
                     /gene="higA"
                     /locus_tag="ACHUT9_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFR1348355.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(18327..20345)
                     /gene="fadH"
                     /locus_tag="ACHUT9_001867"
     CDS             complement(18327..20345)
                     /gene="fadH"
                     /locus_tag="ACHUT9_001867"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFR1348356.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLNVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(20571..22922)
                     /gene="ygjK"
                     /locus_tag="ACHUT9_001868"
     CDS             complement(20571..22922)
                     /gene="ygjK"
                     /locus_tag="ACHUT9_001868"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFR1348357.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(22939..24016)
                     /gene="ygjJ"
                     /locus_tag="ACHUT9_001869"
                     /pseudo
     CDS             complement(22939..24016)
                     /gene="ygjJ"
                     /locus_tag="ACHUT9_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            complement(24150..>24472)
                     /locus_tag="ACHUT9_001870"
     CDS             complement(24150..>24472)
                     /locus_tag="ACHUT9_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348358.1"
                     /translation="ASMLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIA
                     IFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPA
                     SNVD"
BASE COUNT         6190 a         6209 c         6258 g         5815 t
ORIGIN      
        1 ctaaaaatgc ttttttatat tgacttacaa taaatctaca tctctaatac accaactttt
       61 tggaaacaat attgtaagat caaatttata tggtggcgaa aattaatctc ttatcacggc
      121 ttacgccgct tttattcgtg ttcgctcctt ttttggcaca gtcaaatatg actgtttatc
      181 ctatggctgt ttccattaat agtcagggtg aaggcaacgt tcgcgtaata tctaaaagta
      241 atgaagttca gtacatcaag gcgacagtat tccgtatcga taatccatcg acgcctcagg
      301 aaaatgaagt tgagattaaa tccggtgatg caaatcatct ggttgttatg ccacctaaat
      361 tcgctttacc agccggtagt agcaaaactg tgcgctttgt tgcaatggaa ccagagcaaa
      421 aagaaaaaaa ttatcgcgtt aaatttgaag cggttcccag tattgatgac gttgccacag
      481 ataaaaaaga tctctctatg cagttaacag ttaacttaat ttgggggatt gttgttagtg
      541 ttccacctca gcaacctatt gctaagttag aagtaaatgc tgcccaaaaa ttagttaatg
      601 cagggaatca acgcttaaag attttaacga tcgcttattg taaaaataat agcaaagaaa
      661 attgtaagat acagaccgta aataaaaaca tcttccttgg tcaggaaaga aatcttgaaa
      721 gcatatctgg ctacgacaaa atcgttgtca aatataataa ctggatcacc aaagataatg
      781 gcgagtttga actggcggtc cattaattat cattgtaata aacgaaggaa tagtaatgaa
      841 aaaggtattt gcaaaatctc ttctggtcgc agcgatgttt tctgttgcag gctccgcatt
      901 ggcagtacaa aaggatatta ctgtaacggc caacgttgat gccgctctgg atatgacaca
      961 gaccgataac accgcgttgc cgaaagcagt agaaatgcaa tatctgccgg ggcagggtct
     1021 gcaatcttac cagttgatga ccaaaatctg gtctaacgac gttaccaaag atgtaaaaat
     1081 gcagttagtt tccccggcgc aactggttca aagcctggat gctagcaaga ttgttccgct
     1141 gacagtaacc tggggcggtg aagaaattaa agctgatgcg gcaaccactt ttaccgcaac
     1201 caaaatcttt gcttccgatg cgttaactaa cggttctctg gctaaaaacc tgatgtttgc
     1261 tcaaaccacc aaaggtgttc tggagacagg catctaccgt ggtgtagtaa gcatttatct
     1321 gtctcaggat atctaatcca gataacagaa ttaacacgta acaaagggag gggtagccct
     1381 cccttatttt aactgtttta gtatttgtta tggatttcag gattgccatg gataaaaaat
     1441 tactggctct tttgatcctg gcgagtctca gcccggcaga ggcgacatta accgaaattc
     1501 ccgcagggtt tgaggttatt gctcagggac agcaggagta tatcgaggtt tatttttcag
     1561 ggaaaagtct cggtaaatat tatgcaatgg ttaatcttga taccgtaaca tttcttgatc
     1621 cagcaagtct atataacaag ctggaactgg atgtagacga tcagaaaatc gcgcatatag
     1681 tgaaagaaaa attatcgcag ccgctagctc gccacggtga attggcttgc ggttatgtac
     1741 gtactgactc agggtgtggt tttctgaata ccgatacgct ggaaataatc tataatgatg
     1801 aagaaagttc ggcaacgttg tttattaatc cgcaatggaa ttcagctttc gatgcgaagt
     1861 cattatattt aaatccagac aaaaatactg ttaatgcttt tatacatcag caagacatca
     1921 atgttctggc acaggatgat taccaatcgt tgtctattca gggaaacggt gcgctgggaa
     1981 taacagaaaa tagttatatt ggtgcacact ggaatttcaa cggttatgat gcagatgatg
     2041 tcagtgacag taatgctgac gtcagcgatc tctattatcg ttatgatttt ttacgtcgtt
     2101 attatgtgca ggcgggtcgc atggacaacc gcacactatt taatgcacaa ggcgggaact
     2161 ttacctttaa ctttctgcca ctcggtgcaa ttgatgggat gcgtatcggg tcgacactga
     2221 gctatttaaa ccaggcgcaa agccagcaag gaaccccagt aatggttctg ctttcgcgca
     2281 attctcgtgt tgacgcttat cgtaatgagc aacttttggg atcgttttat ctcaatagtg
     2341 gttcgcaatt tattgatacc agttcctttc cgccaggcag ctacagcgtg gcattaaaag
     2401 tctacgaaaa taaccaactc acccgcaccg agcttgtgcc gtttaccaaa accggcggtc
     2461 tgactgacgg aaatgcgcaa tggttcttac aggcaggtaa aactacatca caggtttctg
     2521 atgatgaaag ttcagcttat cagctaggag tacgcctgcc attacatccg caatatgagc
     2581 tctacgcagg gctggcgaat gccgatgatg tgagtgcttt cgagttaggt aataactgga
     2641 cggcagattt aggcggggtg gggaatcttg caatcagcgc cagcgtgttc cgtaacgatg
     2701 acggcggcaa aggtgatatg caacaggcca actggagtaa tccgggatgg ccgacgttgg
     2761 gcttttatcg gaccaactct gacggcgatg cttgtacaac cgacagcaga gagagctata
     2821 acgccttaag ctgttatgaa agtatttccg cgacggtttc acagaatttt gtcggctgga
     2881 atatgatgct gggttatacc cgcacacaaa ataacactga tgatagtttg cgttgggata
     2941 aacagcagag ctttgaaaat aactatcttc gccagacaac agcgcaaagt atctctgaaa
     3001 ctgtacaact tagcgcttcc cgcgcttttg tgatgcgtga ctggattttg agtacgtcag
     3061 tcggtgtttt ccatcgtaat gacaacggtg gcgataacga cgacaacggc ttgtacttat
     3121 cgttttcgtt atctgacacg ccaacgatgg atagcaataa caacagccat tcaacaaatg
     3181 tgtctacgga ttatcgttat agcgaacagg atggcgatca aacgtcatgg cagttatcgc
     3241 atacctttta taacgattca ttcagccata aagaacttgg cgtaaccgtc ggaggcctga
     3301 acaccgatac cataaacagc gcggttaacg ggcgttggga tgggcaatac ggaaatgtct
     3361 acgctaccgt atctgatagt tatgaccgta agaatcatga tcatctttcg gcctttacgg
     3421 gcacttatag ctctacgctg gctgtcagtt gctatggcgt taatttggga gccagtggta
     3481 cagacgattt gctgggtgcg gtactggtgg atgtgaaagg cttctctgaa caggatgaag
     3541 agagtcagga tctgcaactc gaagcgcggg tggcaggcag ccgaactttg cagcttggtc
     3601 aaagtgacag tgtgttgttc ccttatcctg gatttcagtc tggttttgtt gaggttaacg
     3661 acagtagcca gggcaatcag caggggacaa caaacatcat taacggtgca gggaatcgtg
     3721 aattaatgtt gttgcctggc aagctgcgct atcgcgaagt gtctgccagc tttaactaca
     3781 actatatcgg tcgtttatta ctgccggcag cggtgaaaaa attcccgatt gtggggctga
     3841 acagcgccat gttactggta gctgaagatg gaggatttac acttgaaatt aacggcagcg
     3901 aaaaagagct ctatctgctt tccgggcagc aattccttaa gtgtccactg agtgttgtaa
     3961 agaaacgcgc cagcattcgt tacagcggag atgttacttg tagtgtggtg acttattcac
     4021 aattaccgga gtcgattcag gttcaggcac agttgaaaca gcctaaatta cgtggaaacg
     4081 ttcagacggc gcaaagggag gttgcaccat gagaaatcga ttgattgcgg cgatattggg
     4141 cttgtttggc acgctcattg gcgttcaggc agctcctgac gtgaccagtg aaattacgta
     4201 tgatttggca tctggtagag cggattatta cttctggaag gatgaagcgt cggcaggaaa
     4261 taatggatat atgtggtatg aatgttcgta tcctgacctc caacaaacct gtacagctaa
     4321 tggaaatata tcgacagtac aaatctattt aactgaacaa cgcagtggga tgcgttggcc
     4381 ggtaaaactc aaaggattta aaacagccat tgtaagtagc gatgaagcgc cgccaggatg
     4441 caaggggggc aaagggcttc agacgaatct taaggattct aatagatctt catgtacaga
     4501 agatggtcaa cattattata tatacgatac aaagtttctt acgctctacc ttgagcagac
     4561 agagatgaag aatttgccga ttggtggcgt ctggaagggg aaagttaaat tacattcgaa
     4621 cagcccggcc caggactatt tcgcaaatat taccctgaat acgctcgacc ccaaccatat
     4681 tgacgtgttc ttcccggagt tcgcccacgc cacgccaagg gtgcagttag acttgcatcc
     4741 aacaggaagc gttaacggca gcaactacgc gcaagatctg accatgttgg acatgtgcct
     4801 gtacgatggt tttaacggta atgccatcag ttatgaaatc atgctcaaag atgaagggcg
     4861 accagctgca gggcgcagag acggtgactt ctctatctat cgtcaaggag ggaccaccac
     4921 cgacgaggga gaacgcattg attaccgggt caaaatgtac aacccggaaa ccggtgggca
     4981 aattgatgtg cgcaataatg aaaatatggt ctggaacagc attaacctga aacgtgtgcg
     5041 tccggtggta ctgcccggta ttcgctatgc cgtgatgtgt gtgccgacac ccttgactct
     5101 ggcagtcgat aaattcagcg tgatggataa acaggccggg tactacatgg gcaaattgtc
     5161 agtaatcttt acgccttcct tgccaaccat caattgaccc cgcagaccag aaaaaactaa
     5221 acaacgaagc cgcctgataa ggcggcttcg tcattacaac ttacgttaaa cgttaaacgt
     5281 taatgttgcg gtgcgggatc gttatgtgtt tcctgcgcca cctcaatcgc cggtttgttc
     5341 tgcaatacgg tccagataac cagcgcaccc agcaggtcaa atactgccag aactgcgaac
     5401 agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgcaaa cagcgtactt
     5461 gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt acgaccgaac
     5521 acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgggcaaa accaccgata
     5581 cacagcagca taattgcgac atacgggttg gtgaacagac cgatcatacc cgggccaatc
     5641 atcagcacag cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt cacaccaaac
     5701 caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc agcaaacagc
     5761 atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac tttaaacatg
     5821 aacagcggga tccacgcgtt aaaagtaccc caggccggtt ctgccaggaa acgcggcagt
     5881 gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt cttcgccgtg
     5941 ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc atcggtcaga
     6001 tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca gataaagctc
     6061 aatgcaccgg agatgataaa tgccatctgc cagctgtgca tcacgattgc cataccacca
     6121 gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt aaagtagcct actgcgatgg
     6181 aacgctcttt cgccgggaac cattcggagc tggctttcag acccgccggg atcatcgctg
     6241 cttccgcggc accgaccgca ccacgagcaa cagccaggcc accccagcta cctgccagcg
     6301 cggttacacc acagaacacg gcccacagca cagcaaacat tgcataaccg attttcgtac
     6361 ccagcacatc cagtacataa cctgctaccg gttgcatgac cgtataagca gcagaatagg
     6421 ctgcgatgat ataggaatac tgttgggtgg agatgtttaa ctcttccatc agagttggcg
     6481 cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac ggtgccgagc gtcaccagtg
     6541 cgatcatata ccaacgtaac cctttaattt tacgcatgta aaacctcatc gttttgttat
     6601 atccgcagga agcgcggcca tcatcccgcc agaggctgaa gatgttagtt acgaccgggt
     6661 aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa agtttcgtgc taattcggct
     6721 tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa tcggcaattt gcattccgtc
     6781 gacttatgat ttgcgacggc agaaagataa cttgtcatac aactttaaaa ggtgagagcc
     6841 atcacaaatg tgcgaatatt tgtagggaca ttacctgacg acagcgaggc cagtactggc
     6901 gcggcctgca gcgagattta tcactttgag agtaattttt ttaactacgt ttattgatct
     6961 aactcacgaa aatatcttcg gactctggaa attggtgtga taactttgtc agcatcgcac
     7021 cataagcaag ctagctcact cgttgagagg aagacgaaaa tgactccgtt tatgactgaa
     7081 gatttcctgt tagataccga atttgcccgc cgtctgtatc acgactacgc aaaagaccag
     7141 ccgattttcg attaccattg ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt
     7201 aaaaacctgt atgacatctg gctgaaaggc gatcactaca aatggcgcgc tatgcgtacc
     7261 aacggtgtgg ccgagcgtct gtgtaccggt gatgcatctg accgtgaaaa atttgacgcc
     7321 tgggcggcga ctgttccgca cactatcggc aacccgttat accactggac gcaccttgaa
     7381 ctgcgtcgtc cgtttggtat cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc
     7441 tggaacgaat gtaacgaact gctggcgcag gataacttct ccgcgcgcgg catcatgcag
     7501 cagatgaacg tgaaaatggt cggcaccacc gatgacccga tcgattctct ggagcatcac
     7561 gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc tgccgagctg gcgtccggac
     7621 aaagccttca acatcgaaca ggcgaccttt aacgactaca tggcgaagct gggcgaagtt
     7681 tccgataccg acattcgccg ctttgctgac ctgcaaactg ccttgaccaa acgtctggat
     7741 cacttcgccg ctcacggctg taaagtgtct gaccatgcgc tggacgtggt gatgtttgct
     7801 gaagcgaacg aagcggaact ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg
     7861 agcgagcacg aagtggcaca gttcaaaact gcggtactgg tgttccttgg tgccgaatat
     7921 gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc tgcgtaataa caacctgcgt
     7981 cagttcaaac tgctggggcc ggatgtaggc tttgactcca tcaacgaccg tccgatggcg
     8041 gaagagttgt ctaagctgct gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt
     8101 ctctactgcc tgaacccgcg cgataacgaa gtgctgggca ccatgatcgg taacttccag
     8161 ggcgaaggta tgccgggcaa aatgcagttc ggttccggct ggtggtttaa cgatcagaaa
     8221 gacggtatgg aacgtcagat gacccaactg gcgcagctcg gtctgctgag ccgctttgtc
     8281 ggtatgctga ctgacagccg tagcttcctg tcatacaccc gtcacgaata cttccgccgc
     8341 attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac
     8401 ctgctgggcg agatggtgaa aaatatttgc tttaacaatg cgcgtgacta cttcgccatt
     8461 gaactgaact aaggtctggg gttgatatgc aatacatcaa gatccatgcg ctggataacg
     8521 tcgcggtcgc tttagcagat ttggctgaag gcacagaagt cagtgtcgat aaccagactg
     8581 ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc gttaacggat atcgcaaaag
     8641 gggccaacgt catcaaatat ggcctgccga ttggttatgc attggcggat attgcggcgg
     8701 gggaacacgt ccacgcccac aatacgcgca cgaatctgag cgatctggat cagtatcgct
     8761 atcaacctga ttttcaggat ctgcctgcgc aagcggcaga tcgtgaagtg cagatctatc
     8821 gtcgcgctaa cggcgatgtc ggggtgcgta acgagctgtg gatcctgcca accgtgggct
     8881 gtgtcaacgg catcgcgcgg caaatccaga accgtttcct gaaagagacc aacaacgccg
     8941 aaggtaccga cggcgtgttc ctcttcagcc acacctacgg ctgctcacaa ctgggcgacg
     9001 atcacatcaa cacccgcacc atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag
     9061 tgctggtgat tggtctgggc tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg
     9121 gcgatatcga tcctgaacgc gttcatttca tgatctgcca acagcaggat gatgagatcg
     9181 aagccgggat cgagcatttg catcagctgt ataacgtgat gcgcaacgat aaacgcgagc
     9241 caggcaaact cagcgaactg aagtttggtc tggagtgcgg tggttctgac ggtctttctg
     9301 gtattactgc taacccgatg ctggggcgtt tctctgacta cgtgattgct aacggcggta
     9361 ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga gcagttgctg atggaccatt
     9421 gccgcgacga agcaacgttt gaaaaactgg tcaccatggt caacgacttt aaacagtact
     9481 ttattgccca tgatcagccg atctacgaaa acccatcacc ggggaacaaa gcgggcggta
     9541 tcaccacgct ggaagacaaa tcacttggct gtacccagaa agcgggttcc agtgtcgtgg
     9601 ttgacgtgct gcgttacggc gagcgtctga aaacgcctgg gctgaacttg ttaagtgcgc
     9661 cgggtaacga tgccgtagcg accagcgccc tggcgggtgc gggctgccat atggtgctgt
     9721 tcagtactgg tcgcggcacg ccatacggtg gatttgtgcc gacggtgaaa atcgccacca
     9781 acagtgaact ggcggcgaag aaaaaacact ggatcgactt tgacgcgggt cagctgatcc
     9841 acggtaaagc gatgccgcag ttgctggaag aatttatcga caccatcgtt gagtttgcca
     9901 acggtaagca aacttgcaac gagcgtaacg acttccgcga actggcgatc ttcaaaagcg
     9961 gcgtaacgct ataaagtcga aatgagttgt taataaaaaa cggcgtttca taatgtgaag
    10021 cgccgtttgc ttttccatac ctgataagga tttatcatga ccgcgtattg gctggcccag
    10081 ggcgtgggtg tcatcgcctt tctgattggt atcacaacat ttttcaatcg tgacgaacgt
    10141 cgcttcaaaa agcagctttc ggtctatagc gccgttattg gcgtacattt ttttcttctg
    10201 ggcacctatc ccgctggtgc cagtgccatc cttaatgcca ttcgtacatt gattacctta
    10261 cgcacgcgca gcttatgggt aatggcgatt tttattgtgc tgactggcgg aattggcctc
    10321 gcgaagttcc atcatcctgt cgaactattg ccggttatcg gtacgattgt cagtacctgg
    10381 gcgctgttcc gctgtaaagg gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc
    10441 tgggtgattc acaacttctg ggcggggtcg ataggcggca caatgattga gggtagtttt
    10501 ctgcttatga atggcttgaa tatcattcgt ttctggcgga tgcagaaaag gggaattgat
    10561 ccgtttaaag tagagaaaac ccctcccgcc gtagacgaaa ggggttaaac aattaattac
    10621 gcagggcgct atttgccaga cgatcgtctt ccgcctggca agctgccgca gtgaacagca
    10681 cgtcagttga agagttcagc gcggtttcgc aagagtcctg caatacgccg atgataaagc
    10741 cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc gaacatatta caggccagcg
    10801 ggatcagcag cagagaaccc cccgccacgc cggatgcgcc acaggcacac agagaagcca
    10861 ccacgctcaa cagcagcgcc gtgggcagat cgaccgggat acccagcgta ttaaccgcag
    10921 ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat attgatggtg gcacccagcg
    10981 gaatagaaac ggaataggta tcgcgatcca gattcagctt ttcacacagt gccatattca
    11041 ccggaatgtt cgccgcagag ctgcgggtga agaaggcata cacgccgctt tcgcgcaggc
    11101 acagcagcac cagcgggaac gggttacgac gaattttcca ccacaccagc aatgggttaa
    11161 ccaccagcgc caccagtaac atacagccaa ccagcacgac cagcagttgc gcgtagcccc
    11221 acagtgtgga gaaaccggtg gttgccaggg tagaagaaac cagaccaaaa ataccaatcg
    11281 gtgcgaagcg aatgaccagt ttcaccataa aggtgacggc attcgacata tcgttgacca
    11341 ggtttttggt ggtctcgtta ccgtgacgca gtgcgaaacc gaggccaatt gcccacacca
    11401 ggatcccgat gtagttacct ttcagcagcg cgtcgatggg gttggaaacc atgctcatta
    11461 ccagcccgcg catcacttct acaatgcctg acggtggcga aatatcaccc gcgctactgg
    11521 ataagtgcag ggtagaaggg aaggcgaagc tgaaaacgac tgcggccaga gcagcagaga
    11581 aggtacccag cagatagagg aacaaaatag ggcggatatt ggttttctgc ccgtgctggt
    11641 ggttagcaat agatgccatc accagcatca acaccaggat gggggcaacg gctttcagtg
    11701 cgccgacgaa caaagtacct aacagaccaa cagcttccgc cgcgggtttt gatatccatg
    11761 ccagaagaat ccccagaaca aggccgacca ggatttgttt taccaggctg ccatgagcca
    11821 gacgccggaa tagccccggt gaacgttgcg tagtcatttt tcgatccttt cattgtgtta
    11881 tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa
    11941 ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa atgctggatt ttaaagagaa
    12001 gtcatatttt gaaacttaat ggttacattt gtttgacata aacagaggga aggactggct
    12061 actaaggctg attagataac atatgggcaa aactatttgg aatatgagaa cggttgcgct
    12121 cctttaatgt gctatgccat ataaagtcac tggcgcaggg agcgcgcagg gggcggccaa
    12181 tcgccgccgc cccctgcacc cccaggctct ggcgaacaaa atcgccgctg cgcgattccc
    12241 tcagcttatc ccttgcggct accgggtcgg gcgcaaggga acgtccctgt aagatgcgcc
    12301 ctcgccgcgc atccatgcgc gtcgccccgg cctccaggga acgcttcggc gattttgacg
    12361 ccaccaacac ccgtgcggtc tttttgtaaa gagctacaag ccgattacat aattaatagc
    12421 tcacctttgt ctggaatgag ttatctctag gaatggctta atatacagcg aatcattctc
    12481 cccgctgctt atcatgccga taattcaccc aggcgttgat aataaacgtc atcaccagaa
    12541 tgccaaacac cacgcccagc gagacggcga ttggaatatg gtagaagtcg acaatcagca
    12601 tcttgatacc gataaacacc agaatcaccg ccaggccata tttgagcatc gagaaacgct
    12661 ctgctacgcc cgccagcagg aaatacatcg cacgcaggcc gaggatcgca aacaggtttg
    12721 aggtcagcac aatgaacggg tcagtggtca cggcgaagat agccggaatg ctatccacgg
    12781 cgaaaatcac gtcgctcaat tccaccagaa tcagtaccag catcagcggt gtggcgtaca
    12841 gcaatccatt cttacgcaca aagaaatgct cgttgtcgat ggtgtcggtc atgcgcaaat
    12901 gaccgcgtag ccagcgcacc agcggcttgt cgccaatgcc tgattcatct tcatgggcga
    12961 gcgccatctt cacgccggta aacagcagga aggcaccgaa gatatacagg atccagtcga
    13021 actgcgaaat tagccagctg ccagtgaaga tcatgatggt acgcagaaca atcgccccga
    13081 gcacgccata caccagcacg cggcgttgta atgccgccgg aacagagaaa tagctgaaca
    13141 acatcagcca gacaaagacg ttatcgaccg ccagcgattt ctcaatcaga taacccgtga
    13201 gaaaggccag tgcctgtgga tcggcaacgg cgcgaccttc agtttgcacc agataccacc
    13261 agaaagcggc gttaaacagt aacgacagcg tcacccagac cagcgaccag gccgcagcct
    13321 gtttcatggt catggcatgt gccccacgac gcccctgcaa caacaggtcg atagccagca
    13381 taatggcgac aacaacagcg aatccgcccc atagcaacgg cgtgccgaca gtattcatag
    13441 aagttcctta cacataaaaa aacggccaac gtcggaagac gtcagccgct gctttttatg
    13501 catagacctc gccttccggc aaggtctcac ttacaacaaa aaaggaatac gtctgcgtat
    13561 tcctttcggg ttgcccggtg accggatgtg gtttttcaca catcgtaatg acgatcgacc
    13621 ggcaatgaag ttactcccct ttgcaggtaa caaagtatga cagaccattc agccagtcaa
    13681 tggtctgtta catacctttt acactgtttt actttggcaa ttatagtttt acgctatctg
    13741 ccggaaaaat caccccagtc tggcggcgga tctcagtcag cagtttggcg gtgatgcgac
    13801 tgaccgccag ccctggatga tccaccagat gctcatccac cagggtagca aacagctctg
    13861 cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc tggctgccac
    13921 gcggcacgaa gcacactttc tggcattcag acagtttttc aatcaccagc gatcccgctt
    13981 cgccctgaat ctcgctttcc aggacagaat cactgacctt tgagtgctgc aacgtaacgc
    14041 tgaaatcacc gtaatccatc accaccacgc cgtgggcatc tacgccgctt gccaacaaac
    14101 tggcggttgc ctgcacgctt ttcggttcac cgaataatgc caccgccgac gccaggcagt
    14161 aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg ttgggattct
    14221 caccatccag gtaacgttga taccgcgagg aatactggca atagttgaaa aagactttac
    14281 gcaatttgcc gactttcggc agcgcctggc gcagcaaatg aaagttcggc aggctggcgg
    14341 ttttaaatgc ctcaaacagc accacctgat tttcccgcgc acaggcaatg gcggcatcca
    14401 cttccgccag attcgacgcc agtggtttct cgcaaatcac atgaatttta tggctaagga
    14461 aaagttgtgt ctgggaaaag tgcagggcat tcgggctggc aatatacacc gcgtcaatgg
    14521 catcgctttc cgccatcgct tccagcgagg taaacagatg ctcgacagaa aaatcattgg
    14581 cgaagtgctg ggcctgttca aggctgcggg aatatacggc ggttaacttg tatttaccgc
    14641 tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc acagcgaaac
    14701 gtatcataac gcgtggatac tccataaagg gattctgtcg tcaatgtagc acgccttttt
    14761 catcttcgac gtgcgcagat acgcattatt ctttcgccgg acagccatgc tcactaaacg
    14821 gcccaatcat gaccgatggc ccgagatatt tcggctggcg atggaggatt ttggcgtcaa
    14881 tcaccgcgct gacacgagcg agtttctccc gcagttgagt ataaaaaccg tgcttcatat
    14941 taaggtctgg cgcgttaaac ccgatagcgt cgatgccgta ctgttttgcc agccagatgg
    15001 cgcgttggtt atgaaattct tgcgagataa tggtgatatg gttttcccca aacacctttt
    15061 tggcacgcac cactgaatca agcgttgaga atccggcata gtcgcagaag atcactttcg
    15121 ccggtacgcc tttagcgatc aaagcctgct gcatacctga ggcttcatcg taatttttac
    15181 gtccgttatc gccactgact aacagccatt tcacttttcc gacgtggtac aacgctgctg
    15241 cggtatcaat acgtcgggta aagtagcgat tacccggcct cgcccccagc aataaaccca
    15301 cgttacgcgc cggaactgcg ttgacatcgc tccaggtcag ctgcttgctg gcgttgacca
    15361 ttacgcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag caggtaatct
    15421 ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg gggctgcatg gtcgttatcc
    15481 ttaacgaaag gggggaggtc gattttaggc gctaaagaaa gaaaagataa cgcctgacat
    15541 cattttcaga ttttgtcctg cccaagcgac aactgcgtta gccacagccg ggtgtcgaac
    15601 tcaagctggt ggtactgcgg ctccatatgg cagcacaact ggtaaaacgc tttgttatgc
    15661 tctttctcct tcaggtgcgc cagttcgtgc accacgatca tgcgtaaaaa cggttccggc
    15721 gcgttgcgaa acacagtagc gacgcggatc tctgcttttg ctttcagctt gccgccctgc
    15781 acacgagaaa cagcggtatg taatccgagc gcgtttttca gtacgtggat cttattgtca
    15841 tacatcactt tagtgatcgg cggggcattg cgcagaaact gattttttag atcctgggta
    15901 tattgccaga gggctttatc ggtggcgtag tcgtgtgttc ccggatagcg ttttgccagc
    15961 acatcgccca gacgctgttc gttaatcagc gttcgcacct gggaaagtag ctgctcggga
    16021 taaccctgaa gataagtaag attgctcata accgcccacg aaaaagagaa aaagggtata
    16081 ctcacgcacc cttttcaggg gcaacgtcga aattttatca ttcaggaggg ccgatgagcc
    16141 acttagacaa cggtttccgt tcactgacac tacaacgttt tccggcgacg gatgacgtta
    16201 acccgctaca ggcgtgggaa gcggcggatg aatatttgct gcaacagttg gacgacacag
    16261 aaatccgcgg cccggtgttg atcctgaatg atgcctttgg tgcgttaagc tgcgcgctgg
    16321 cggaacataa gccgtacagc attggcgact catacatcag tgaactggcg acgcgcgaga
    16381 atttacgcct caacgggatt gatgagtcga gcgtgaagtt tctcgacagc accgccgact
    16441 acccgcaaca gccgggtgtg gtgctgatca aagtgccgaa aacactggca ttgctggaac
    16501 agcaactgcg tgcgctgcgc aaagtggtca cgtcggatac acgtattatt gccggtgcca
    16561 aagcccgtga cattcacact tccacgctgg agctgttcga gaaagtgctc ggcccgacca
    16621 ccactacgct ggcatggaaa aaagcgcgcc tgattaattg cactttcaat gaaccgccgc
    16681 tggccgatgc gccgcagacc gttagctgga agctggaagg tactgactgg actatccaca
    16741 accatgcgaa tgtcttctcc cgcaccgggc tggatattgg cgcacgcttc tttatgcaac
    16801 atctgccaga gaatctcgaa ggtgagattg tcgatctcgg ttgcggtaat ggcgttattg
    16861 gtctgacgct gcttgataaa aaccctcagg cgaaagtggt gtttgtcgat gaatcgccga
    16921 tggcggttgc ttccagccgt ttgaacgttg aaaccaacat gccagaggcg ttagatcgca
    16981 gcgagtttat gatcaacaac gcgctctccg gcgtggagcc tttccgcttt aatgctgtgc
    17041 tctgcaaccc gccgtttcac caacaacatg cgctgaccga taacgtcgcc tgggagatgt
    17101 tccaccatgc ccgccgctgc ctgaaaatca acggcgagct gtatattgtt gccaaccgtc
    17161 acctggatta cttccataaa ctgaagaaga ttttcggcaa ctgcaccacc atcgccacga
    17221 ataataaatt tgtggtgctg aaagcagtga agctggggcg tcgtcggtaa gattgggagt
    17281 tacctgccgg atgcggcgtt aacgccttat ccggcctacg ttcagcacgt tactcccagg
    17341 ccggataaga cgcggcaagc gtcgcatccg gcaatgtctg cccagttctg gaatcgcgct
    17401 tccataaaac aaaaaatccc tctcaccatc ctctacattc tcttgtttag cgtttttcta
    17461 cgtttattct tccgtcacac agataaattc atccgttgcc aatctgtcaa cggatgttat
    17521 catattgcca atttgtcaac ggacgtgatg aagaatgcac ctgataactc aaaaagcatt
    17581 gaaagatgct gcggaaaaat acccgcaaca taaaacggag ttggtggctc tggggaacac
    17641 gattgctaag ggatatttca aaaaacctga gtcattaaaa gcagtattcc catctctgga
    17701 taacttcaaa tatctggata agcattatgt tttcaatgtt gggggcaatg aattacgtgt
    17761 tgtagcaatg gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta tgacgcataa
    17821 agaatacgat ttctttaccg ctgttcatcg tactaagggg aaaaaatgat tgcgattgcc
    17881 gacatcttgc aagcaggaga aaagctaact gcggtggcac cttttctggc gggtattcag
    17941 aacgaggaac aatacaccca ggcgctggaa ctggtagatc atctgctgct caacgatcct
    18001 gaaaacccct tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga atcagcgccc
    18061 gaatttgcgg aatttaatgc catggctcaa gccatgcctg gcggtatagc cgtgattcgt
    18121 acccttatgg atcaatatgg tttaaccctt gccgatctgc cggaaattgg cagtaaatct
    18181 atggtgtcac gcgttttgag cgggaagagg aaattaacgc tggaacacgc taaaaaattg
    18241 gcaacgcgat tcggcatttc tcccgccttg tttattgatt aacgcgacgt gccggatgcg
    18301 ttgcatccgg cgcttaaaat aacgatttaa atctccagcg ccagccgtgt tccctgggca
    18361 attgcccgtc gtgcgtccag ctccatagcc acatcgcagc cgccaattaa atgcacagtt
    18421 ttcccgctat caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc tgcgcagatc
    18481 accacattgt ccactgctaa tacctgcgtt tcgccgttga tcaccacatg cagcccgtca
    18541 tcgtcaatct tctgataact tacgcctggg atcattttca caccccgcga gagcagggtg
    18601 gtgcgatgga tccagccggt ggttttgcct aacccctgtc ccggtttgct ggctttgcgc
    18661 tggagcatca caatttgccg tgggctacgc gggatctgca ttccctgcgg gcttaagcca
    18721 ccagcctgtt gtaggctgct gtcgatcccc cactcattac agaacctggc gatattctgg
    18781 ctggtggatt cgcccggctg acttaaatac atcgccgtat caaaaccaat cccgccacaa
    18841 ccgatgatgg caaccttgtt gcctaccggc gctttgtcgc gcagtacatt gagataactc
    18901 aataccttcg gatgatcgat cccgtcgata ggcggaacgc gcggcacgat cccactggcg
    18961 aggatcgttt catcgaacgc ctgtaactga tccgccgtca cggtgtgatt gagtttcagc
    19021 gtcacgcccg tcacttcgat catccggtgg taatagcgca gcgtttcgta aaactcctct
    19081 ttgccgggga tctgtttggc gatattaaac tgcccgccga tctcgctgtg ggcatcaaac
    19141 aatgttacct gatgcccacg cgccgccgcg ttaatggcaa acgccagccc agcaggtccc
    19201 gcaccgacca ccgccagatt ttttttctgc acggcaggaa ggattggcat tttggtttcg
    19261 tggcaggcgc gaggattcac caggcacgag gtgactttgc caacgaagat ctgatcgagg
    19321 caggcctgat tgcagccaat gcaggtgttg atctcatcgg ctcgccccga ttgcgctttt
    19381 gacagcagtt ccgcatcagc aagaaacggt cgtgccatcg ataccatatc ggcatcgccg
    19441 cgcgagagaa tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt taccagcggc
    19501 agcgagacgt ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg cggcacgggc
    19561 gtggcaatgg tcggaatacg tgcttcatgc cagccaatgc cggtgttgat aatggtcgcg
    19621 cccgccgctt caatggcctg cgccagctct accgtttcgg caaaagtccc gccgtcttcg
    19681 accaggtcga gcatcgacag tcggtagata ataatgaagt cgttgccgac gcgttcgcgc
    19741 accgcacgca ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc gccccactgg
    19801 tcactacgct gattggtgcg cagcgtcaga aattcgttga tcaaataccc ttcggacccc
    19861 attacctcta caccgtcgta tcctgcctcc cgtgccagtt gcgcgcagtg ggcgaaatcg
    19921 tcgatcagtt gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg gttgatgggg
    19981 gcctgcaatg cggagggagc gaccagatgc ggttggtagc tgtagcgccc ggtatgcaaa
    20041 atttgcaggg ctattttgcc gccttcctga tgtaccgctt cggtaatggt gcgatggtgt
    20101 gggatctggc tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc tgttaaatct
    20161 ggtgcaatgc cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc ggcataaaac
    20221 gctgccagcc gctcggcacc gtccgggtat tcctccagcc cggtgtgcat tgagcccatc
    20281 aacacgcggt tttttaacgt ggtaaaacct aaatccagcg gggcgaacag cgacgggtag
    20341 ctcataaaat tgtccagtat gtaaaataat tgttatgtgg tcggatgagt tctaatttag
    20401 ccgtcgatgg agaaaaggga aaaggggagt gcggggtttg tgatgggatt caaaaagaat
    20461 acgcccggtg gtaacgccgg atgcaacgct tgccgcgttt tatccggcct gggggattgc
    20521 acatgtaggg cggataaggc gttcacgccg catccgccag ccgttgcaaa ttactgctta
    20581 cggaaaaaat cgttatacaa catatacaaa tgagcggcac tccaggagaa atttggtgcg
    20641 ccttgctgtg cgccagtcag cgggttgtaa ttctcctgaa ttgggccatc ggcggttaat
    20701 cctttggcgt gccggaagaa cgtatccgcc agcttcaggg catcatcacg ataaccgtaa
    20761 cgctccatcc ctttcagacc aaaccagaac tgatccaccc atacgcgccc acgccagtag
    20821 atatcagcgc caaaagccgg attggttaac gccgccgttc ccagcgggac aaaggtgttg
    20881 aactctttag gatcgagcat caccttcacc accgcgtcgg cgttggcctg cgttgccgca
    20941 ccgttaaaca gcggcgacca gccttccggc cctttaccgc gctcaacaat cggtttgccc
    21001 gcgcagccgt tcgccagtgg tttatcttca atacgtacgt catagtagaa ctgggtagtc
    21061 gggtcgaaca tacaggtgtt gatgtagtcc gcgagctgct gtgccaactg gcgatagcgt
    21121 ttggcctctt ctggcttacc gaggatggtt gccatctccg ccagataatg gttatcgctg
    21181 tacatatagc tggcctgatc caccgactcc tgcaatagcg agtagcccag caacgttccg
    21241 tcctgactgc ggttttcggc gaatttcacc gtccagtcgc tacgtttgcc gccattagcg
    21301 acatatttat ccagctgttc tttgtcgata aacccaaaga cggcggcatc atcacgcccc
    21361 gattcccacg atgcagcaac ctgtgccgga atttccagac tgtcgtacag gcctttctcc
    21421 accacgcggg cgtagttgtt cagcccagac tgcgtctctt ctttatcgcc ttttttcacc
    21481 gtaaacagca tctcgccgct ctcagtgttg tgggctttgt cgcgggtcgc gccatattcc
    21541 ggcacgccgt tgccgttgtg atcgcggtta cgtaaccacc agtcgtgata ggccaccagt
    21601 ttcgggtaca tctctgccaa ccaggcttta tcctgggtga cgttgtacac ttccatcacc
    21661 gaccaggcgg caaggctggg tttggtatta cgttcgttcc agttgccgcc atcgccacca
    21721 cgctcggggc taaggttcca cgctatcagg tcggggacaa agcccacatc ctgcggacgc
    21781 acgctatcgc caggctggat ctgccaggag aagaccgcgc ggatattctc tttggcgatg
    21841 tccggattga aatgcgccat cgcaaacgcc tgcttccagg tatcccacgg ccaggtctga
    21901 ttgccggaga accagcgccc ggtcaccgac ggtgtaacgg tgttaaattt caccgcaccg
    21961 cccggtgaga gccagttacc gttgagcgtt tcgatcgctt tcaccgcgac gcgcgtctgt
    22021 tccggcgtcg catccggatt ggttaaccct ttcttcaggt attcttccca gcgttgctgc
    22081 gaggcggtga gataaaacgc cggacgcgcc agaatatcgc ggatctgcat ttgctctttg
    22141 ctaacttcct gagcggtcag cagatgggaa taggtggtgt agagcgtggt cgaaccgttg
    22201 atatgcgcct tactggtaaa gcgattaccg ttgatttcag tctgcaccgg cagggattta
    22261 tgcacctgat attccgattc gccggaggtc agcagatccc aggtggcgcg cactttgcca
    22321 aaggtgactt tcaggccatc acgggtggcg ctgattttgc gctgatagtc agggtattcg
    22381 ccagcaatgg ttttatcgga aagcggtttc ccttctttcg cttccagttt ttccagcagt
    22441 tcgccgtccc acaccagatc cagcggttta ttgctggtga ttttggtttc cagtagtgac
    22501 gtgcgcggcg tggcgaagcg cagagtcatt tcgacttgca catcttttgc tgtcagtttt
    22561 tgcaccagcg caccgggaat actgtatgcc tccagcgtaa agtcgacttt cttgccgtcc
    22621 tgccagacgg tcaggcggtc gaaattgctg gccataaagt tgatgtactc ttccgtcagc
    22681 agcgcaacgc ccggaaagcc gcccatggtg ttagggccgt ctggcaacag atgaccatgc
    22741 caggctccga gatcgaaaaa cggattaaaa cgctggtgat cgtcgtaatc gtaatccttc
    22801 atgtactgcg gcgcgccagt acggttaatc acgtttttat aattgtcggc gttagcggca
    22861 tgggcggaaa aacttatcag cagagcgcag gttactggcg ttaaaatagt ttttattttc
    22921 atgaaattac agtccttatt accagtagaa atattgcatc aggaagattt ggtcgctggg
    22981 atcgtgagag gcatttttca tgtagaagcg gctatcaaaa gccgtggcga aacgtccgcc
    23041 gccgtattcg taccagatac caaactgcat gaaagaggtg gtttcatcgt cagcgtggtc
    23101 agggcgatgt ttggcgtagg tgtaagcggt ggagagataa acgcccttta acgcttcata
    23161 catggcgctc accatcatcc cactttcact tcccggtacg tccagcccgt cggcgcgtcc
    23221 ggtatggtgc caggcgcgag cggcaaagtt aggagttagc aggcccgtga gatagagctg
    23281 tcctgtgccg tcggtgattt ccagaccgtt catccaggtg atgccttcgg caaggtcata
    23341 ctggatgtaa ccgttcatca ttgccggata ggtgtatttg tcgtcatagc gatcgtattt
    23401 gccaaaatgt agccaggctt tgctttcatc atgatgtccg gcaggagtcg cggtgacgct
    23461 ataacgaaag ttgccgctta agttctgtac cttcagcgca gtcataatgt cgcgggtatt
    23521 gggaatgacg tagcccagat cgggggtgaa atcgccccac cattgcaggt catccagcga
    23581 ggaatcctga cgcagactga gcatgatttc ggtgccatca ttggtacgat aaccgccata
    23641 gaaacggtta attccgccct cgaaaccacc ccagctatgg tctggcaccc aggcattgcc
    23701 gtcgtggtcg gcctgcacgg tccagccttc gccgtaaatc aggccgaacc aggagccgta
    23761 cttcatctcc aggccacctt caatataggt accgtcgctg tatttatgtt tattctcacc
    23821 ctctaaatcc atatatccac caccccctaa tccctaattc gccgtaaaaa cgaaatgaag
    23881 gagaagatgt cgaggtaatt gcgggtgttt ttgtgtcgtc atgttctgcc ggacgtgctt
    23941 cttctcctgc cgcagaaaag gcgtagcaaa acggcagagc aagtaatgct ctgcatggtg
    24001 cagtgattaa tttcattgtg ttaatccatg agctaaagaa aaagtacagc cttttatcgt
    24061 tgtaattata aaaatataaa ggcagaaact attttccctc tcctcatcca ggaagaggga
    24121 aaatgcctgc tcatttttta ttattctgct taatcaacat tgctggctgg cgtcgcttca
    24181 atatggcgct cttcagccgt taatcccttt atatatttac tgtatttcca ccacgcaaag
    24241 ccaaggaaga taacaatacc gccgacgtta taaaaaatga tggtcagaat attcgcgcca
    24301 gtcgggaatg tcgaagcgac aaaccctacg gcaaatatcg caatcagcat tgaaacaacg
    24361 ataataccgg tgcggcggga gcccatacgg aaatcgcgtg gcaggtgatc taatttggcg
    24421 cgtaaattca ggtaagccag catgatgaat aacggcggaa gcatggacgc tg
//
LOCUS       DAZPEP010000046        24354 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZPEP010000046 DAZPEP010000000
VERSION     DAZPEP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24354
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.046"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="ACHUT9_001871"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="ACHUT9_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFR1348359.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="ACHUT9_001872"
     CDS             1828..3147
                     /locus_tag="ACHUT9_001872"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFR1348360.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3519..4901
                     /gene="dosC"
                     /locus_tag="ACHUT9_001873"
     CDS             3519..4901
                     /gene="dosC"
                     /locus_tag="ACHUT9_001873"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFR1348361.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            4926..7325
                     /gene="dosP"
                     /locus_tag="ACHUT9_001874"
                     /gene_synonym="pdeO"
     CDS             4926..7325
                     /gene="dosP"
                     /locus_tag="ACHUT9_001874"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFR1348362.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7584..8165
                     /gene="ddpX"
                     /locus_tag="ACHUT9_001875"
     CDS             7584..8165
                     /gene="ddpX"
                     /locus_tag="ACHUT9_001875"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFR1348363.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            8179..9729
                     /locus_tag="ACHUT9_001876"
     CDS             8179..9729
                     /locus_tag="ACHUT9_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1348364.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9731..10753
                     /locus_tag="ACHUT9_001877"
     CDS             9731..10753
                     /locus_tag="ACHUT9_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1348365.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10750..11646
                     /gene="ddpC"
                     /locus_tag="ACHUT9_001878"
     CDS             10750..11646
                     /gene="ddpC"
                     /locus_tag="ACHUT9_001878"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFR1348366.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11643..12629
                     /locus_tag="ACHUT9_001879"
     CDS             11643..12629
                     /locus_tag="ACHUT9_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1348367.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            12658..13554
                     /locus_tag="ACHUT9_001880"
     CDS             12658..13554
                     /locus_tag="ACHUT9_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016153094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFR1348368.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIAIARALSSQPDVIVLDEPT
                     SALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQ
                     QVLTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHG
                     CEVRQSLAIREDGRELRCWRAL"
     gene            complement(13610..14041)
                     /gene="osmC"
                     /locus_tag="ACHUT9_001881"
     CDS             complement(13610..14041)
                     /gene="osmC"
                     /locus_tag="ACHUT9_001881"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFR1348369.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14386..14601
                     /gene="bdm"
                     /locus_tag="ACHUT9_001882"
     CDS             14386..14601
                     /gene="bdm"
                     /locus_tag="ACHUT9_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFR1348370.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14703..14840
                     /gene="sra"
                     /locus_tag="ACHUT9_001883"
     CDS             14703..14840
                     /gene="sra"
                     /locus_tag="ACHUT9_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFR1348371.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            14997..16694
                     /gene="maeA"
                     /locus_tag="ACHUT9_001884"
     CDS             14997..16694
                     /gene="maeA"
                     /locus_tag="ACHUT9_001884"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFR1348372.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            16828..17830
                     /gene="adhP"
                     /locus_tag="ACHUT9_001885"
                     /pseudo
     CDS             16828..17830
                     /gene="adhP"
                     /locus_tag="ACHUT9_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            17976..18260
                     /locus_tag="ACHUT9_001886"
     CDS             17976..18260
                     /locus_tag="ACHUT9_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1348373.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18488..19141)
                     /gene="fdnI"
                     /locus_tag="ACHUT9_001887"
     CDS             complement(18488..19141)
                     /gene="fdnI"
                     /locus_tag="ACHUT9_001887"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFR1348374.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19134..20018)
                     /gene="fdnH"
                     /locus_tag="ACHUT9_001888"
     CDS             complement(19134..20018)
                     /gene="fdnH"
                     /locus_tag="ACHUT9_001888"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFR1348375.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(20031..23078)
                     /gene="fdnG"
                     /locus_tag="ACHUT9_001889"
     CDS             complement(20031..23078)
                     /gene="fdnG"
                     /locus_tag="ACHUT9_001889"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22491..22493),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1348376.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23310..24191
                     /gene="yddG"
                     /locus_tag="ACHUT9_001890"
     CDS             23310..24191
                     /gene="yddG"
                     /locus_tag="ACHUT9_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFR1348377.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5987 a         6166 c         6137 g         6064 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gcttttttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg gggccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggca cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttatctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcaaagacc ttcttccctg acttctctaa agtgggcacc ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcatc cgcaacccac gtcaatgaaa
      841 tgagcaaccc agggcgcgac tatccgttgg ctatgttact gctgatggta gcggcaatct
      901 gcttaagctc tgttggtggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcacagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcta ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ctctaccgat atgtatgttg
     1501 aattactggt cgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgctcgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcatatatg gatatctgct cccgaaacga gaaattagcg
     1861 attagaagac cagcgatact ggttgcactg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggtgt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taaccccacc agccgggccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct gcaacgtctc ggtataaaca cggtcttttt ccaggttaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccat ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattta tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgca gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgag tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgca aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaagt atcgcacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gctgacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccga ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact cccatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtccta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtca atctcgcacg ttgcgttttg
     3481 attccgtctg atttcccagc cttataaggg tgtgaattat ggagatgtat tttaaaagaa
     3541 tgaaagatga gtggaccgga cttgtcgaac aggcagatcc actcattcgt gctaaagccg
     3601 cggaaattgc cgttgcgcat gctcattatc tgagtatcga gttttatcga attgtccgca
     3661 tcgacccgca tgccgaagaa ttcttgagta atgaacaagt tgagcggcag ttgaaaagtg
     3721 cgatggaacg ctggattatt aacgtgcttt ctgcccaggt tgacgatgtc gaaaggctaa
     3781 tacaaatcca gcataccgtc gcggaagtgc atgcccgcat aggaattccg gtagaaattg
     3841 tcgagatggg gtttcgggtg ctgaaaaaaa tcctctatcc ggtaatcttc tcttcggatt
     3901 attccgccgc agaaaaactt caggtctatc atttctcgat taacagtatt gatatcgcga
     3961 tggaagtgat gacccgcgcg tttaccttta gtgacagtag tgcctcaaag gaagatgaaa
     4021 attatcgtat cttctcgtta ctggaaaacg ccgaagaaga aaaagaacgg caaatagcct
     4081 caatactttc atgggaaata gatattatct ataaaatcct gctggattct gatttaggca
     4141 gtagtttgcc attaagccag gctgattttg gcctgtggtt taaccataaa ggtcgacatt
     4201 attttagtgg cattgccgaa gtaggccata tctcccgtct cattcaggat ttcgacggta
     4261 ttttcaatca aaccatgcgt aacaccagga atttgaataa cagaagtctg cgggtgaaat
     4321 ttttattaca gataagaaat accgtatcgc agattattac cttgctgcgt gaattgtttg
     4381 aagaagtatc gcgccacgaa gtcggtatgg atgtactaac gaaattactt aaccgccgtt
     4441 tcctgccgac tatcttcaaa cgcgaaattg cccatgccaa ccggaccggt acaccgctgt
     4501 cagtgctgat tattgacgtt gataaattca aagagatcaa cgatacgtgg ggccataaca
     4561 ctggcgatga aattctgcgt aaagtctctc aggcctttta tgacaacgtc cacagtagtg
     4621 attatgtttt tcgctacggg ggcgatgaat ttatcattgt tttgactgaa gcttctgaaa
     4681 acgaaacgtt acgtaccgca gaacgtattc gcagtcgggt ggagaaaacc aaactgaaag
     4741 ccgcaaacgg cgaggatatt gccctctcac tttccatcgg tgccgccatg tttaatggtc
     4801 atcctgacta tgagcgcctc attcaaatag ccgatgaagc tctgtatatc gccaaaagac
     4861 gaggtagaaa ccgtgttgaa ctctggaaag ccagtcttta gatgcgccag gatgcagagg
     4921 taatcatgaa gctaaccgat gcggaaaatg ccgccgatgg catttttttc cccgcccttg
     4981 aacaaaatat gatgggcgcg gtgttaatta acgaaaatga tgaagtgatg tttttcaacc
     5041 ccgccgcaga gaagctctgg gggtacaaac gtgaagaagt cattggcaat aacattgata
     5101 tgctgattcc gcgggatttg cgtcctgcgc atcctgaata cattcgtcat aaccgtgaag
     5161 gtggtaaagc gcgcgtcgag gggatgagtc gggagctgca gctggagaaa aaagacggca
     5221 gtaaaatctg gacccgtttt gcgctatcga aagtgagcgc cgaggggaaa gtttattacc
     5281 tggcgctggt acgggatgcc agcgtagaaa tggcgcaaaa agaacagacc cgacaattga
     5341 ttattgccgt tgaccatctc gaccgaccgg tgattgtcct cgatccggaa cgccatattg
     5401 tgcagtgcaa tcgcgcattt accgaaatgt ttggttactg cattagcgaa gccagcggta
     5461 tgcagcccga tacactcctg aacattcctg aattccctgc cgataaccgc attcgtttac
     5521 aacagttgct atggaaaacc gcccgcgatc aggacgaatt tctgctgttg acacgcaccg
     5581 gtgaaaaaat ctggattaaa gcctctatca gcccggttta tgacgtgctc gcgcatctgc
     5641 agaacctggt aatgaccttc tcggatatca ccgaagaacg gcagatccgc cagcttgaag
     5701 gcaatattct cgccgccatg tgcagcagcc cgccatttca tgaaatgggg gaaatcattt
     5761 gtcgtaacat cgaatctgtt ctcaacgaat cgcatgtttc gctgttcgca ctgcgcaacg
     5821 ggatgccgat acactgggcg tcatcttcgc acggtgcaga agttcaaaat gcgcaaagct
     5881 ggtcagcgac cattcgtcag cgtgatggtg cgcctgcggg gatcctgcaa attaaaacct
     5941 cgtcaggagc agaaaccagc gcctttatcg aacgcgtggc agatatcagc cagcatatgg
     6001 ccgcgctggc gctggaacag gaaaaaagcc gtcagcatat tgaacaactc atccaatttg
     6061 atccgatgac cggtctgcca aatcgcaata acctgcacaa ttacctcgat gaccttgtcg
     6121 acaaagccgt ctctccggtg gtgtatctca tcggtgttga ccatattcag gatgtgattg
     6181 atagccttgg ctatgcgtgg gccgatcagg cattgctgga agtggtcaat cgctttcgtg
     6241 aaaaactcaa accggatcag tatctctgtc gtatcgaagg tacgcagttt gtcctcgtga
     6301 gcctggaaaa cgacgtcagt aacattaccc aaatcgccga tgagctacgg aatgtggtca
     6361 gcaagccgat aatgattgac gataaaccat tcccgctaac cttgagtatt ggcatcagct
     6421 acgacgtggg taaaaaccgc gattacctgc tctccactgc tcacaatgca atggattata
     6481 ttcgcaagaa tggcggtaac ggctggcagt tcttcagccc ggcgatgaac gaaatggtaa
     6541 aagagcgttt ggttttaggc gcagcgctga aagaagcgat tagcaataac caactgaaac
     6601 tggtttacca gccgcaaatc ttcgcagaaa cgggtgaact gtacggcatc gaagcccttg
     6661 ctcgctggca cgatccccag catggtcatg tacccccttc acggtttatt cctctcgcag
     6721 aagagattgg tgaaatcgaa aatattgggc gctgggtcat cgcggaagct tgccgtcagt
     6781 tagcagaatg gcgtagccag aatattcata tcccggcgtt atccgtgaac ttgtcggcgc
     6841 tgcactttcg cagtaatcag ctgcctaatc aggtgtctga tgcaatgcaa gcctggggta
     6901 ttgacggcca ccagctgacg gtagaaatca cggaaagcat gatgatggaa cacgataccg
     6961 aaatctttaa gcgcattcag atcctgcgcg atatgggcgt gggcttatcg gtagatgatt
     7021 ttggcacggg cttttccgga ttatcccgct tagtcagtct tccggtaacg gaaatcaaaa
     7081 ttgacaaaag ttttgtcgat cgttgtctga cggaaaaacg catccttgcc ttacttgaag
     7141 ccattaccag cattgggcaa agcctcaatt taaccgtcgt ggcggaaggc gtcgaaacca
     7201 aagagcaatt tgagatgcta cgcaagatcc actgtcgcgt tattcaggga tatttctttt
     7261 cccgccccct acccgccgag gaaattccag gctggatgag cagcgtgtta ccgctgaaaa
     7321 tctgacaaat tcctctcgcc cgcactcgcg ggttttcatt taacgtgaca ctgtcactta
     7381 aatactgtga tttcagctac gattccggga gattccttcc ttaacttccc ctcttgcacc
     7441 aaaactgcac tacgctgcac aaatgttgat caaaagttaa aaaatataac tttaagccag
     7501 cattctggcg cttatcccgg catggcatga gatctgcata agcggaaagc gcagcaattt
     7561 ttgtcttaat acaggaaccg tttatgtcgg ataccaccga actggttgat ttagccgtga
     7621 tcttccctga tctggagatc gaattgaaat acgcctgcgc tgataacatc acaggtaaag
     7681 ctatttacca gcaagcgcgt tgtctgttac acaaggatgc gattaccgcg ctggcgaaaa
     7741 gtatcagtat cgcccagctg tcaggcttac aactggtgat ttacgatgcg tatcgcccac
     7801 aacaagcaca ggcgatgttg tggcaagcct gcccagaccc gcaatatgtt gttgatgtga
     7861 cggtcggttc taatcacagc cgtggcacgg cgatcgacct gacgcttcgt gatgagcacg
     7921 ggaacatcct ggatatgggc gcagggttcg atgaaatgca cgagcgctcc catgcctatc
     7981 acccttccgt cccgcccgcc gctcagcgca atcgcctgtt gctgaatgcg ataatgactg
     8041 gtggcggttt tgtcggtatc tccagcgaat ggtggcactt cgaattaccc caggcagcga
     8101 gttaccctct gctcgctgat caattctctt gttttatatc ccctggcaca cagcacgtca
     8161 gttaatcagg aaactgtcat gaagagatcg atatcgtttc gtcccacatt gctcgcgctc
     8221 gtccttgcca caactttccc ggttgcgcac gccgccgtac cgaaagatat gctggtgatt
     8281 ggtaaggccg ccgatccaca aaccctcgac ccggcggtaa caatagataa taacgactgg
     8341 acagtgacct acccgtctta tcagcggctg gttcagtaca aaacggacgg tgataaaggc
     8401 tcaaccgacg ttgaaggcga tctggcaagt agctggaaag cgtctgacga tcaaaaagag
     8461 tggacgttca ccctgaagga caatgctaaa tttgccgatg gcacacctgt cactgccgaa
     8521 gcagtaaaac tctcttttga gcggttacta aaaatcggcc aggggccagc agaagcattt
     8581 cccaaagatt taaagattga tactcccgac gaacatacgg tgaagtttac ccttagccaa
     8641 ccattcgcac cgttcctcta cacgctagcg aatgacggtg catccattat caatccggcg
     8701 gtcttaaagg aacatgcagc ggatgatgct cgcggcttcc tcgcgcaaaa taccgccggt
     8761 tccggaccat ttatgctgaa aagctggcaa aaaggtcagc aattagttct ggtgccaaat
     8821 ccgcattacc ccggcaataa accgaacttt aagcgagtat cggtaaaaat tatcggtgaa
     8881 agtgcctccc gtcgcctgca gctctcccgt ggcgacattg acattgccga tgcgctgccg
     8941 gtggatcaac tcaacgccct gaagcaggaa aataaagtca atgtggcaga gtatccgtca
     9001 ctgcgcgtta cctatctgta tctgaataac agcaaagcgc ctcttaatca ggcggatctg
     9061 cgtcgggcca tttcctggtc taccgattat cagggcatgg ttaacggcat tctgagtggt
     9121 aacggaaaac agatgcgcgg cccgattccg gaaggcatgt ggggctacga tgcgacggca
     9181 atgcaataca accatgacga aacgaaagcc aaagccgaat gggataaagt gacgagcaaa
     9241 cccaccagcc tgacgtttct ctactccgat aacgatccga actgggagcc tattgctctg
     9301 gcgacacaat ccagtctcaa caagctgggc atcaatgtga agctggaaaa gctggcgaac
     9361 gccaccatgc gcgacagagt gggtaaaggt gattacgaca ttgcgattgg caactggagt
     9421 ccggattttg ccgacccgta tatgtttatg aattactggt ttgagtcaga caaaaaaggt
     9481 ctgccgggta accgctcgtt ctatgaaaac agtgaggtcg ataagttact gcgcaatgcg
     9541 cttgcgacca ccgaccagac gcagcgtacc cgggactacc agcaggcaca gaaaatcgtc
     9601 attgatgacg ctgcttatgt gtacctgttc cagaaaaact accaactggc gatgaacaaa
     9661 gaggtgaaag gctttgtgtt caatcccatg ctggaacagg tcttcaatat caataccatg
     9721 agtaaataac gtgaccttct ggagtatttt acgccaacgc tgctgggggc tggtgctcgt
     9781 ggtggcgggc gtctgcgtga ttacgtttat tatctcgcat ctgatccctg gcgatccggc
     9841 gcggttactg gcgggtgacc gcgccagcga tgctatcgtg gaaaatattc gccagcaact
     9901 gggactggac cagccactgt atgtacagtt ttaccgctac ttcagcgacc tgtttcatgg
     9961 tgacctggga acatccattc gtaccgggcg tccggtgctg gaagagttgc gtatattttt
    10021 cccggcgacg ctggaactgg ctttttgtgc cctgctgctg gcactcctga ttggcatccc
    10081 gctgggcata ctctctgcag tctggcgaaa tcgctggctg gatcatctgg tgcgaataat
    10141 ggccattacc ggaatctcca cacctgcgtt ctggcttgga ctgggcgtca ttgtgctgtt
    10201 ttatggtcat ctgcaaattc ttcccggcgg cggaaggctt gatgactggc tggatccacc
    10261 aacgcacgtt accggctttt atctgctcga tgcgctgctt gaaggcaacg gtgaagtctt
    10321 cttcaatgcg ttgcaacatc tcatcttacc ggcattaacg ctggcgttcg ttcacctggg
    10381 aattgtcgct cgccagatcc gctcagcgat gctggaacaa ttgagtgaag actacattcg
    10441 taccgcccgg gccagcggct tgcccggctg gtatatcgtt ttatgttatg cgctacccaa
    10501 tgcgttgatc ccatcgatta ccgtattggg tctggcgctg ggcgatttgt tgtatggcgc
    10561 agtgctcacc gaaaccgttt ttgcctggcc cggaatgggt gcatgggtag taacatcaat
    10621 acaggcgctc gacttcccgg cagtgatggg ctttgccgtc gtggtttcat ttgcttatgt
    10681 gctggtcaac ctggtggtgg atttgctcta tttgtggatt gatccgcgta tcggacgtgg
    10741 aggtggtgaa tgatgctaag cgaggaaacg tccgccgtac gcccacaaaa acaaacgcga
    10801 tttaacggtg caaaactggt atggatgctg aaaggcagtc cgctcaccgt gaccggcgca
    10861 gtcatcattg tattaatgct attgatgatg attttttcac cgtggcttgc gacgcatgat
    10921 cccaacgcca ttgatttaac cgcccgcctt ttgccgcctt ctgcggcgca ctggtttggc
    10981 accgatgaag tgggacgcga tctgttcagc cgcgtactgg tcggcagtca gcaatcaatt
    11041 ctcgccggat tagtcgtggt tgccattgcg ggtatgattg gttcgctact cggatgtcta
    11101 tccggtgtgc ttggcggacg cgcagacgcc atcatcatgc gcatcatgga cattatgctg
    11161 tcgattcctt cgttggtact gacaatggca ctggctgccg ctctcgggcc gagtttattt
    11221 aacgccatgc tggcgattgc tattgtgcga attccctttt atgtgcgcct ggcgcggggg
    11281 caagcattag ttgtacgcca gtatacctat gttcaggcgg cgaaaacctt tggtgcgtcg
    11341 cgttggcatc tgatcagctg gcatatttta cgtaactccc tgccgccgct gatcgtgcag
    11401 gcatcgctgg atatcggtag cgcgatttta atggccgcca cgttgggatt tattggcctg
    11461 ggtgctcaac aaccgagtgc tgaatggggc gcgatggtag ctaatggtcg caactatgta
    11521 ctcgatcagt ggtggtattg cgcatttccg ggggcagcga ttttgcttac cgccgtcggg
    11581 tttaatctct ttggtgatgg tattcgcgat ctgcttgacc cgaaagcagg aggaaagcag
    11641 tcatgaccca acccgttctg gacattcaac agctgcattt gagtttcccc ggttttaacg
    11701 gttatgttca cgtgctcaac aatgtgtcct tgcagattaa ccgcggtgaa attgtcggtc
    11761 tggtgggaga atccggctca ggtaaatcag tcaccgcaat gctgattatg cgtctgctac
    11821 cgacgggcag ttattgcgta catcgggggc agatttcact gctgggagac gatgttctta
    11881 acgctcggga gaaacagctt cgtcagtggc gcggcgcacg agtggcgatg atctttcagg
    11941 aaccgatgac cgccctcaat ccgacacgtc gaataggtct tcagatgatg gacgtgatcc
    12001 gccatcatca accaataagt cgtcgggaag ccagagctaa agcgattacc ctgctggaag
    12061 agatgcaaat cccggatgcc gtggaagtta tgtcgcgcta tccgtttgag ctttcaggtg
    12121 gtatgcgcca gcgggtaatg attgcgctgg cattctcctg cgagccgcaa ttgattattg
    12181 ccgacgaacc gactacggcg ctggacgtca cggtacagtt gcaggtactg cgtttgctta
    12241 aacataaagc tcgcgccagt ggaactgcgg tactgttcat cagccatgat atggccgtgg
    12301 tgtcgcaact gtgcgatagc gtttacgtga tgtatgccgg aagcgtaatt gaaagcggcg
    12361 tgacggcaga cgttatccat catccccggc atccgtatac cattggtttg ctgcaatgcg
    12421 caccagaaca tggagtacca cgccagccat tacccgccat tccagggacg gtaccaaacc
    12481 tcacccattt gcctgacggc tgcgcttttc gcgatcgttg ctatgcggca ggtgcacagt
    12541 gtgaaaacgt cccggcgctg acagcgtgtg gtgacaacaa ccagcgctgc gcctgttggt
    12601 atccgcagca ggaggtcata agtatctgac acgttattaa cgttacgcga cgtccatatc
    12661 aatttcccgg cccgtaaaag ctggcttggt aaaactacgg aacatgttca tgcgattaat
    12721 ggtattgatt tacagatccg ccgtggtgaa accttaggga tcgtcggtga gtcaggctgc
    12781 ggcaaaagca ccctcgcaca gcttttaatg ggtatgctgc aaccgagcca cgggcaatac
    12841 atccgttcag gatcacaacg cattatgcag atggtgtttc aggacccgct ctcttcgctt
    12901 aatccgcgct tatcggtgtg gcgcatcatc acagaaccgc tctggatagc taagcgtagt
    12961 agtgaacaac agcggcgagc gttggcaaag gagttggctg tgcaggtggg tattcgtccg
    13021 gagtatctcg accgcctgcc tcatgcgttc tccggcgggc agcggcaacg catcgccatt
    13081 gccattgcca gagcactctc ttcgcagcct gacgtgattg tgcttgatga gccaacctcg
    13141 gcgctggata tctccgtgca ggcgcagatc ctcaatttac tggtaacgct acaggaaaat
    13201 cacgggctga cttatgtgct gatttcacac aatgtctcgg tgatacgtca tatgagcgat
    13261 cgggtggcgg tgatgtatct cgggcagatt gtagaactgg gcgacgcgca gcaggtgctg
    13321 acggcacctg cacatccata cacccgatta ttgctggatt ccctccccgc cattgataaa
    13381 ccgctggagg aagaatgggc attatgtaaa acggatctgc cgggaaaccg cacgttgcca
    13441 caaggctgtt ttttctacga acgttgccct ctggcaaccc acggatgtga agtccggcaa
    13501 tcattagcaa taagggagga cggacgtgag ctccggtgct ggcgggcgct gtagagatgc
    13561 taacaacgca tcaggcattc acgcgcctga cgcgtcatcc ggcaatgctt tacgatttca
    13621 actggtaatc cagcgtaatt tccgctttca gtacctgaga aaccgggcat cctgcttttg
    13681 ctttctggat gatgccgtca aaggtagagg catcaatacc cggcaccgca acttcactct
    13741 tcagtgcgat tttcgtaatc gcaaaatcgg catccacttt atccagcgac acatcggcgg
    13801 tggtatcaat cgatgttggc gtgaatcccg cttcccccag cattaatgaa agcgccattg
    13861 agaaacatgc ggcatgcgct gcgccaatca gttcttcagg gttggttcct ttttcgcctt
    13921 caaaacgcgt gttaaatcca tacggctgtt ggttcagcac gccactctgg gtggatactg
    13981 ttcccttccc gcgtttgata tcgccttccc agtgtgcctg acctttctta tggattgtca
    14041 ttgttgctct cctgtgggct taaaaaggaa attatagact acgtgagaaa cagcacgagg
    14101 ataagcagga tattccgaat aaaatacggc gaaaatatta cttctgcact taaattaaat
    14161 tcccacaata gataaccggg aatatatatc tcaagagata ttgcatcaat atcaatcaac
    14221 tcaccacaat aataatacat atcaatatca tggagttaaa atataaaaat catcaaccag
    14281 gactaatctt aacaacgaag cggcaaatat ttgccatgct gaatgtgctt tataaatctg
    14341 cgatccgtag cagacaccat aaatacacag acacggagaa tcactatgtt tacttattat
    14401 caggcagaaa attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg
    14461 gcagagcaag gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa
    14521 gcttctgata acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt
    14581 ggccagcacc ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag
    14641 cacgtgtttc ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta
    14701 aaatgaaatc gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc
    14761 agcgcaaaat agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa
    14821 aacgccccgc tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg
    14881 cctgcgcaat ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt
    14941 aaagcaaaga cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg
    15001 aaccaaaaac aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg
    15061 aatttccgtt gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc
    15121 tgctggggtt actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga
    15181 tccagtatca gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg
    15241 acaccaacga aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg
    15301 ttatttatac cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt
    15361 cacgcggcgt gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg
    15421 taccgaacca taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg
    15481 gtgaccaggg catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct
    15541 gtggcggcat cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc
    15601 aacaactgct taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg
    15661 aatactatga attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc
    15721 tgttgcagtt tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca
    15781 atgaaatttg ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac
    15841 tgatcgcagc aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg
    15901 gcgcaggttc agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag
    15961 gattaagcga ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga
    16021 cggacaagat gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc
    16081 tcagtgactg ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa
    16141 aaccagatat tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc
    16201 gtgagatgca taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac
    16261 gcgtggaagc cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca
    16321 cgggcagccc gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta
    16381 acaacgcctt tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta
    16441 tcaccgatga gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc
    16501 tgaacggcga aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa
    16561 ttgcgtttgc ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg
    16621 aagctttgca acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc
    16681 gtacctccat ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt
    16741 tttcagatct catccatact gggtagtggc gaataaatct catttgcctc acctgctatg
    16801 cagaacatca tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat
    16861 gttgacgtta cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg
    16921 gagtgttgtg gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa
    16981 accgacgtaa ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc
    17041 acctcattaa aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat
    17101 tgcgaatact gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac
    17161 agcgttgatg gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg
    17221 tctggactcg gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt
    17281 taagctgtca aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg
    17341 taacctcgcc ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt
    17401 caatgatgag cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg
    17461 caccgaagac gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt
    17521 aacagcagta gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg
    17581 tgttgtggct gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct
    17641 ggatggcatt gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt
    17701 ccagtttgcc gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat
    17761 caacaccatc tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt
    17821 ccgtcactaa gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc
    17881 caacatgaaa tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc
    17941 ccagagaagg aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc
    18001 cgggggacat tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca
    18061 gagcaatgga aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga
    18121 cgccagaaat ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga
    18181 atctgcaaaa cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac
    18241 gccgtctggt tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca
    18301 aacgcaacat tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg
    18361 tatttgaagg gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc
    18421 cacgttgtct acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca
    18481 aaagagatta tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat
    18541 accagcgcgg atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt
    18601 taatcgaccc tttcacccag aatgccatat acatatggat caggatggcg tggatcagga
    18661 tgatacccgc agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact
    18721 gcgcaaagta cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca
    18781 tgatcgacca gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt
    18841 gctcattgcc ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg
    18901 ggatgttgtg atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc
    18961 cgaagaacgg gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt
    19021 gcagcgtcgg gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca
    19081 cggtccagtg acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca
    19141 tgatgatcct cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa
    19201 atcaacccgg caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc
    19261 cacaggctta ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc
    19321 tgatcggcgt gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag
    19381 acgccagcat gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc
    19441 agcatctcct tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc
    19501 ggttcctggc cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc
    19561 tctttgttga ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg
    19621 cagttttccg actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac
    19681 gggcacgcct tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc
    19741 cactccagct tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg
    19801 ctcagatcgg cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg
    19861 ttccactccg aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata
    19921 agttttgcga cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt
    19981 gcggaccttt tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc
    20041 tcgatgttga ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc
    20101 ggcgtcagag tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt
    20161 ggaataccca ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt
    20221 accaccgcca ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta
    20281 ttaatgcctt tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca
    20341 attgcgttga gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc
    20401 gtacccacat acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga
    20461 cgaacaaccg ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca
    20521 atcggctcgt agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt
    20581 cccatccctt ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg
    20641 aagtccggaa tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc
    20701 ggatcccacg gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg
    20761 agcggccacg cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg
    20821 cggttagcca tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat
    20881 gcggttgtac cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca
    20941 atcagcacgc cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc
    21001 acttcgtcag attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt
    21061 tctacgcctt taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc
    21121 agaatttcgc cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc
    21181 aaccagcgac cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga
    21241 cggaatactt cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc
    21301 cagaaggtag aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc
    21361 aggcagctca ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag
    21421 aagtaaccgg tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc
    21481 tggtcccact tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca
    21541 tagaaagatt tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga
    21601 tcagccagcg tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt
    21661 tctgacggaa gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc
    21721 ccctgaatgt tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta
    21781 ccgagcagca actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc
    21841 gtccagccca gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc
    21901 acttcacaca ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc
    21961 ggcgtgtagc gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt
    22021 gtttcatcgc gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca
    22081 tactggcgtt tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca
    22141 cgcaccagca ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt
    22201 tcgatcaggt agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt
    22261 gcgtaaatat ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt
    22321 gcatcgttgt tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca
    22381 gcgttaccgc ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc
    22441 atcgcaccgc gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc
    22501 gcctggttgt ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg
    22561 gtttcgttgc tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt
    22621 acgccctgct cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca
    22681 atacgggaga atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga
    22741 tattccgggt agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttt
    22801 gggcacagcg caccacggct taccggatga tccgggtcac cttcaatgtg ataaatcgct
    22861 tctctggcgt ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa
    22921 cagtatgtgc aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga
    22981 gccagtgctt gcttcggggc aaagcccaat gccgctaccg ttgttccagc cataccgccc
    23041 gcgcagattt taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg
    23101 tattactgcg cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag
    23161 aacacgtgta tgccacaaaa taatccgcct gcgcaaatta acctacctca atagcggtag
    23221 aaaaacgcac cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt
    23281 ttaacggcaa gagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc
    23341 taatagcgat cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc agcgaggggc
    23401 tcggtccggt cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca
    23461 cggttggatt tccgcgtatt cggcagattc cgaaaggcta tttactcgcc gggagtctgt
    23521 tattcgtcag ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc
    23581 aggcgattga agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca
    23641 ttctgtttaa tggtcagaaa accaactggc tgattgtacc aggattatta ttagccctgg
    23701 tcggcgtctg ctgggtgtta ggcggtgaga atgggttaca ttatgatgaa atcatcaata
    23761 atatcaccac cagcccattg agttatttcc tggcgtttat tggtgcgttt atctgggcag
    23821 cctattgcac agtaacgaat aaatacgcac gcggatttaa cggaattacc gtttttgtcc
    23881 tgctaacggg agcatgtctg tggatttact attttctcac gccacaacca gaaatggtat
    23941 ttagcacgcc cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg
    24001 ctgcatggaa tgtcggtata ttgcatggca atgtcaccat aatggcggta ggctcgtatt
    24061 ttacgcctgt actttcctca gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct
    24121 cgttctggca aggcgcgcta atggtctgcg gcggttccct gctctgctgg ctggcgacac
    24181 gtcgtggtta aaataattgc cgggtctacg gcccggcagt taatcagata aattccgaca
    24241 atattaaaaa ataaatttca aataccagta attcacctta tatctcattt cgacttcgat
    24301 cacatatcga taaataatat tatttcatct atttttctca ttgtttttaa tcac
//
LOCUS       DAZPEP010000047        24187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZPEP010000047 DAZPEP010000000
VERSION     DAZPEP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.047"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            72..209
                     /locus_tag="ACHUT9_001891"
                     /pseudo
     CDS             72..209
                     /locus_tag="ACHUT9_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(317..1552)
                     /locus_tag="ACHUT9_001892"
     CDS             complement(317..1552)
                     /locus_tag="ACHUT9_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1348378.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1699..1775)
                     /locus_tag="ACHUT9_001893"
     tRNA            complement(1699..1775)
                     /locus_tag="ACHUT9_001893"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1739..1741),aa:Pro,seq:tgg)
     gene            complement(1818..1904)
                     /locus_tag="ACHUT9_001894"
     tRNA            complement(1818..1904)
                     /locus_tag="ACHUT9_001894"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1868..1870),aa:Leu,seq:cag)
     gene            complement(1925..2000)
                     /locus_tag="ACHUT9_001895"
     tRNA            complement(1925..2000)
                     /locus_tag="ACHUT9_001895"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1965..1967),aa:His,seq:gtg)
     gene            complement(2059..2135)
                     /locus_tag="ACHUT9_001896"
     tRNA            complement(2059..2135)
                     /locus_tag="ACHUT9_001896"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2099..2101),aa:Arg,seq:ccg)
     gene            complement(2238..3623)
                     /locus_tag="ACHUT9_001897"
     CDS             complement(2238..3623)
                     /locus_tag="ACHUT9_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1348379.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3814..4554)
                     /gene="wecG"
                     /locus_tag="ACHUT9_001898"
     CDS             complement(3814..4554)
                     /gene="wecG"
                     /locus_tag="ACHUT9_001898"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFR1348380.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4557..5909)
                     /gene="wzyE"
                     /locus_tag="ACHUT9_001899"
     CDS             complement(4557..5909)
                     /gene="wzyE"
                     /locus_tag="ACHUT9_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFR1348381.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWMALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLIIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5906..6985)
                     /gene="rffT"
                     /locus_tag="ACHUT9_001900"
                     /gene_synonym="wecF"
     CDS             complement(5906..6985)
                     /gene="rffT"
                     /locus_tag="ACHUT9_001900"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFR1348382.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6982..8232)
                     /gene="wzxE"
                     /locus_tag="ACHUT9_001901"
     CDS             complement(6982..8232)
                     /gene="wzxE"
                     /locus_tag="ACHUT9_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFR1348383.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8234..9364)
                     /gene="rffA"
                     /locus_tag="ACHUT9_001902"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8234..9364)
                     /gene="rffA"
                     /locus_tag="ACHUT9_001902"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFR1348384.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9369..10043)
                     /gene="rffC"
                     /locus_tag="ACHUT9_001903"
                     /gene_synonym="wecD"
     CDS             complement(9369..10043)
                     /gene="rffC"
                     /locus_tag="ACHUT9_001903"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFR1348385.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10021..10902)
                     /gene="rfbA"
                     /locus_tag="ACHUT9_001904"
     CDS             complement(10021..10902)
                     /gene="rfbA"
                     /locus_tag="ACHUT9_001904"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFR1348386.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10921..11988)
                     /gene="rffG"
                     /locus_tag="ACHUT9_001905"
     CDS             complement(10921..11988)
                     /gene="rffG"
                     /locus_tag="ACHUT9_001905"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFR1348387.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11988..13250)
                     /gene="wecC"
                     /locus_tag="ACHUT9_001906"
     CDS             complement(11988..13250)
                     /gene="wecC"
                     /locus_tag="ACHUT9_001906"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFR1348388.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13247..14377)
                     /gene="wecB"
                     /locus_tag="ACHUT9_001907"
     CDS             complement(13247..14377)
                     /gene="wecB"
                     /locus_tag="ACHUT9_001907"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFR1348389.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14433..15479)
                     /gene="wzzE"
                     /locus_tag="ACHUT9_001908"
     CDS             complement(14433..15479)
                     /gene="wzzE"
                     /locus_tag="ACHUT9_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFR1348390.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15491..16594)
                     /gene="wecA"
                     /locus_tag="ACHUT9_001909"
     CDS             complement(15491..16594)
                     /gene="wecA"
                     /locus_tag="ACHUT9_001909"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFR1348391.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16834..18093)
                     /gene="rho"
                     /locus_tag="ACHUT9_001910"
     CDS             complement(16834..18093)
                     /gene="rho"
                     /locus_tag="ACHUT9_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFR1348392.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18178..18279)
                     /gene="rhoL"
                     /locus_tag="ACHUT9_001911"
     CDS             complement(18178..18279)
                     /gene="rhoL"
                     /locus_tag="ACHUT9_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFR1348393.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18420..18749)
                     /gene="trxA"
                     /locus_tag="ACHUT9_001912"
     CDS             complement(18420..18749)
                     /gene="trxA"
                     /locus_tag="ACHUT9_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFR1348394.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18880..20145
                     /gene="rhlB"
                     /locus_tag="ACHUT9_001913"
     CDS             18880..20145
                     /gene="rhlB"
                     /locus_tag="ACHUT9_001913"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFR1348395.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20279..21763
                     /gene="gppA"
                     /locus_tag="ACHUT9_001914"
     CDS             20279..21763
                     /gene="gppA"
                     /locus_tag="ACHUT9_001914"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFR1348396.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21810..23831)
                     /gene="rep"
                     /locus_tag="ACHUT9_001915"
     CDS             complement(21810..23831)
                     /gene="rep"
                     /locus_tag="ACHUT9_001915"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFR1348397.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24048..>24187
                     /locus_tag="ACHUT9_001916"
     CDS             24048..>24187
                     /locus_tag="ACHUT9_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348398.1"
                     /translation="MAINFSPKVGEILDCNFGNYPVSQNGQFSTTYYDGRIPPEMIKN
                     RLV"
BASE COUNT         6071 a         6635 c         5990 g         5491 t
ORIGIN      
        1 gggagaggga tctcaattat caatgcttaa ttacgtcatc attttgatga catgaaaccg
       61 gagcattttg catggaattt tcgttttcac ccaaacttct tgttgttgct gtcgccgccg
      121 ctcttcctct catggccagc gcagcagata ccccgtcaac tgccaccgca cgcaaaggct
      181 ttgccggata cgatcaccca aaccagtatc cacagaacgc tgcaatttat tgaatttgca
      241 cgatcatgta ggccggataa ggcgtttacg ccgcatccgg caatcaaccg caggcggcca
      301 caaaatggcc gcttttttac ttattcacca ccagcaaatg tgcctgcata atgtcactgg
      361 ccgggcgacc gtgcgccagc aaatcagcca ttgctttaag atatggcggt agatggcgga
      421 aataacgctg atacccggca cacaaataat tcagccccgg tttgccgctg gcatcgagca
      481 tgaagcggtg tttcgggcag cctccccagc acgcttttaa cacgttacaa ctgcgacact
      541 gcgccggtaa ttgcttaaat ttatcttcac caaacacctg ctgttgcggg gaatcgatca
      601 tttctgcaat tgtttgctga tgcatattcc ccagccgata ttgcggataa acatagtgat
      661 cgcaggcgta aacgtcgccg ttgtgctcaa caatcaccga gcgcccacag gttggctgat
      721 gatggcaaac cgcacccggc gcaccgacaa aattggcaaa cgcccattcg atattcatca
      781 cgaaaatctt gccgacgtcg cgtttgatcc agtggtcgaa tatcgccacc agaaattcac
      841 caaattcatc ggggcgcacc gaccattccg tcagttcccc ctgaatatca ccaggcgcat
      901 gcaatttcag tccatcgcgg gcagctgttt catcagccag acgctcgacc accggaataa
      961 actggatgaa ttcgactccc gcatcgcaca aaaaatcata aacctgcaac ggttgctgcg
     1021 cgctggtgcg gttgacgcag accagcacgt tatagtcgac atgatgtttt tgcagcagcg
     1081 tcagggcacg catcaccagc ttatgcgttg gtctgccacc tttggtcacg cgatattgat
     1141 tgtggatctc agccggaccg tccagcgata acccaacaag aaaatgattt tctgccagaa
     1201 atgcacacca tttatcatcg agcagcacgc cgttagtctg gaagctgtta cttatcttcc
     1261 tgccagcacc gtatttcgcc tgtaacttta cggcacggcg gtaaaaatcc agcccgagta
     1321 acgttggttc gccgccctgc caggtaaaag ccacttcgtt ttgcggttcg ctggcagcga
     1381 tatagtgacg gacatacgct tccagcgtgt catcgtccat atgcgtgact ggcttttcgc
     1441 ggtaaaggga ttgtttttcg agataaaaac agtagtcaca gttcagatta caatcggaac
     1501 cactcggttt cgccatcaca tgaaaagcac gcgttggaac ctgttgcagc atggtcactc
     1561 cttgcctgaa gtcatccttc aatacgttaa gggcgaaatc ataccgtggg gcaatgagtt
     1621 attacgggcg tttattgcga agggagggtg acgaaatgca cagaaaacaa aaaaccccgc
     1681 cgaagcgggg ttcaaaattg gtcggcgaga gaggattcga acctccgacc cactggtccc
     1741 aaaccagttg cgctaccaag ctgcgctact cgccgaaata ctgctttttg aatttttagt
     1801 tcaattcttt aaagtcgtgg tgcgaggggg gggacttgaa cccccacgtc cgtaaggaca
     1861 ctaacacctg aagctagcgc gtctaccaat tccgccacct tcgcattgtc acaacttcta
     1921 ataatggggt ggctaatggg attcgaaccc acgacaactg gaatcacaat ccagggctct
     1981 accaactgag ctatagccac cactacaaat cttgttacgc ggtattacta ccaccgcagc
     2041 tcaagcgccg ggactaaatg gcgcgcccga caggattcga acctgagacc tctgcctccg
     2101 gagggcagcg ctctatccag ctgagctacg ggcgcttagc gccgttgcgg gggtggataa
     2161 tacggacttc ccacccctct gtctagtccc tttttaaata aattgcgcgt ttggttacgg
     2221 tttgtgcgtt ttgctgctta ttcctccagt ttatgcgctt tcccgtggcg attaaggcca
     2281 aaaactttat aaatcgccgt caccgccagc ataaagatga tgccaacaaa cagcgacata
     2341 cgcgtatctt cattaaagta catgccgatc aaaacgcaaa tcaggaatgc cattgttacg
     2401 tagttggccc acgggaacag gattgagcgg aacggatggc tggcaatcgc cgctttatgt
     2461 gcacgccgaa aacgcagctg gcttatcaat atcacaaacc atggcaccat ccccggaagc
     2521 acgctggcac tgtagacgta gacaaacaca cgctgcggat tgggaatgat gtagttcagg
     2581 catgagccaa ttagcagaat agcaatagat actgccacac ccgcaactgg tacgccgtga
     2641 cgggaaactt tcgccatcgc cgccggtaac tgacggtttt tcgccagtgc gtagagcata
     2701 cgtccgcaac tgtacatgcc gctgttacag ccagagagcg cagccgtcag caccacaaag
     2761 ttgataatgc ccgccgctgc ggtaataccg attttggcaa aagtcagtac gaacgggctg
     2821 ccgttgctgc caatttcatt ccacgggaag atggtgacga taacgaaaat cgcgcctacg
     2881 tagaaaatca ggatccgcca cagcactttg cctacagcac tgcgcagcgt cacctgcgga
     2941 ttcttcgctt caccggcagt aatgccaatc agctccacgc cctggtagga cgccaccaca
     3001 atacacagcg cggtcaggaa ccctttccag ccacccgcaa agaaaccgcc atgctctgtg
     3061 agattgctaa aaccaatcga ctgcccgcca ttgccaaagc caaagaaaat cacgcccagg
     3121 ccaatgacaa tcatcacgat aatcgtggtg actttgatca tcgcgaacca gaactcgatt
     3181 tcgccgtaca accgcaccgc cgccagattc gccaacgcca ccagcgccac cgcgatcaat
     3241 gcaggtatcc actgcgccat ctccgggaac cagaactgga cataaacgcc aatggcggtg
     3301 atttctgaga tccccaccgc catccacata aaccagtaag accaggcggt gagataacca
     3361 aaaaacgggc tcatataacg atgcgcataa acggcgaacg aaccggtaac aggttcaagg
     3421 aacaacattt cgcccattga acgcatgatg aaaaagacga acagcccggc gatgatatag
     3481 gccaacaata cggatggccc ggcccatttc agggtactgg cggcccccat aaacaggccg
     3541 acgccaatag tgccccccag ggcgatgagt tcgatatgtc gagcttccag cccacgctgt
     3601 agctctggtt tgttatctgc cataaatcct cgtgttgtgt ttgcatgctt tccggtgtta
     3661 ccggttatcg ttatgggtac atcgagtgtt gcgaatgttt tcgtaattca ggagaaatgg
     3721 caaataaagc attaaaaatt tgaatgcttt gtgtaataaa aaagcagaca ggcgacggag
     3781 tgaccactcc gtcgctttac aaagagagga aaatcagagg ttgccggtgt agtgccagcg
     3841 taaataacgc agcaaacgaa gctgacgctt aatgcggctc ggctgcgaaa gcaggcggta
     3901 gagccactcc agtcccagcg tttgccagat tttcggtgcg cgttttacgt gaccggtgaa
     3961 aacatcgtaa gtcccgccaa cgcccatata cagcgcatct ggatgcacca gacggcagtc
     4021 gcgcatgaag atctcctgct ttggcgatcc catcgcaacg gtgacgattt gcgctccgct
     4081 ggcatgaatg cgttcaaaca gcgcctgacg ctgctcgggt ttaaaataac catcctgact
     4141 gccaacgata ttcacattcc attggttgcg cagtttagct tcagtttgcg ccagcacttc
     4201 aggtttaccg cccacaagaa ataccggcgt cccttctttg cctgcgcgcg ccatcagctc
     4261 ttcccagaga tcggcaccgg caacgcggga aacctgcgcc tgcgggtact ttttacgtac
     4321 tgaacgtaca acgctgatgc catccgcata tttaaattcg gcagcgttaa ttaactccct
     4381 gacctcggcg ttatcttcaa tggtcagcat tttttcagca ttaatggcaa ccagcgttcc
     4441 ctgcttaagc tgcccgtcag caaacagata atcgagggcg tgctgcatat cacgccaacc
     4501 aatcaactgt aagccacgca gcgtataggt cggtgccgtg gtgttgttat tcattgttat
     4561 ccttcaacct gcgtccggag cgatgatttt gtacgtttat gaatgagtcc ggcgctttca
     4621 aaaagccagt acagcagttt tgcgattatc agacatgcgc cgaagaccac gataaaaaag
     4681 actacgcgtg agacaaacga atccagccct tcacgcgcca gcacgatcat attgaagatc
     4741 gccccaaagc agaaactgtg caatatcgca gccttatagc gattaggctc gcggttgccc
     4801 agctcataca gccagtcgaa ccatttgatg atcagaccaa ccacgatcgc cccgagcggg
     4861 atgaacaacg cgccgcccat caccaccagt gagcctataa gcgtaggcga gatcgccagt
     4921 ccggagtggt tattcagcac ttcccaggta aagtagttgg ctgagttcag caccatactc
     4981 gggcgacccg gccacagcca ggaagggata aagacataga aatcgcggac aattggagcc
     5041 aggccctgga agtcgatgtt gtcgtagttc tgcaacagca acgccagatt ctcccacggg
     5101 gagaaggtgt cgcgagtgag atagagaaac gtatagaacg cttcatcgcc gctcacattc
     5161 attccatagc gttttagcgc catccagaac atgccaacaa tccccagcac gcccgccgcc
     5221 gccagcatcc acaacgaaat ccagccgcga ataatgccaa taaacaggaa gatagcgaat
     5281 gcgatgatga tattggcgcg agtgccgccg acaatcatat aagtcagcaa gccaaaggcg
     5341 accgtgctga cgaggaaaaa cagccacgct ttgctgtcct ggcgcagaaa gtagaccacc
     5401 agcatcgccg ggatgaaaaa gtaaaagaag cgttttaacg ccacgccgga gacttcactg
     5461 gagaagatct gactgtagga gttaagccgg aacagcaaaa agccgttgtg catgaagaag
     5521 atgccgacgc ttaccagcgc gatacccatc aggatcaccc acgtaagatt ggtctccacg
     5581 cggttcatgg taaacagcgg acggcgcggt acatcagcaa cgcgtttgcg taggcgggtt
     5641 ttgtaggtga catagtaaac cgcgtagaag cagcccgcag aaagcaacgc ctgcaacaag
     5701 atttctggcg gcgcgacacc aacatcaaag cgaaatacca gcacgctggt cagcgggaag
     5761 ccgaagaaaa aggtgagcaa aaacagcaat gaaaagaaga cattgaagtt aaagcgcaca
     5821 cggcgaaact caaaccaggt cagcgtggca ataaacagcg tgcagagcag ccagaccaca
     5881 aacaggccac tgaattgcag cagactcatg cgacctcccc ggcggcaatc gccaacgccc
     5941 gctgccagcc ttgtagatag ttagggctaa agaaggcaat ggtgttttta tccaccgacg
     6001 ccaactggcg ctgcgcttca cgcacaatat cctcgttgag atcgtcggta gtaaacagca
     6061 ccggcaaatg ctgttccgtc atatcctgcc agaacggatt ttcccggtta agcacacaag
     6121 gaatgcccgc ctgaatcagt aagcaaagcg taccaatgcc ctgctggcgg gcaaaaataa
     6181 agtaaccaag atcgcactga cgaagtagcg ccagataggc gtcaaattcc agtttttcgc
     6241 tcaaaatttg cagattttct tcgctgaata actccagccc cgcctgacga acttcctcaa
     6301 tgtacgcttc gttattaggc ggatatccca tcggcaccac cacttttacc gtatcgccaa
     6361 attgctgatg aacggcgcgc aaggcagcaa tatggtcatt gctgcggtca ccggagttcc
     6421 ccaccagaat ggtcattttc ccttcacgtt gccgatcatt cgccatcgta ttgagagaag
     6481 ggtccatccg cgtcgggaag tacagcagtt cgccccgcac ctttggatgc gttttggcaa
     6541 aaaagctcaa atcaccgcgg gtggcaaata cacagccgac tcgcttttgc gccaggcgac
     6601 gtagcgggta aaaaagctta tatctcaagc cactggaaag ctcatacagg tctgcccccc
     6661 agatatgcca gaaaaactgg ctgggcttaa tgccaccact cagcagagcc agccacagtg
     6721 tgggattgaa ctgaccgtgg aaaaaaaatc gctgctgacg gttagctttt gctttcgcga
     6781 tgaccgcttc cgccagcgat tttttcccag ggaaaaattg cacagaaagc gccggacagc
     6841 tatcacttaa gccgtcgtcc ttgccaacaa ccataaactc gcgcgcgtgc tcgctcgtcg
     6901 cggccagcgc gtcattgaaa aaccgcaaaa cggttcggtt atggtgaggg atatccgatc
     6961 ccagtacgtg aatcagtaca gtcatgcccg cctacgccag agtaaaaaca cgccacaaca
     7021 aagagaaaaa tagacgatat aagttgccat atatgcctgc gccgcgccca gtgcaccatg
     7081 cgcagggatt agccagtggg caaataccat caataaagtg aactggctga cttccgccag
     7141 aatataaaac cgcagtgacg ctttggcgat caccagataa ccaaagacat aagcgcccac
     7201 ttttaacaca tcacccacta actgccaggc aaagagatcg cgcatagcgg taaatttatt
     7261 cgacaacagc aaccagatag caaaatcacg cagcaaccag acggtaaaac tcgccgccgc
     7321 cactgccggt aagacgaatt tcagcgattt aaccacttcc cgggtgatat cgcgcttttc
     7381 cgttagccgc gacaacgtgg gcagcaaata tacgctgaac gatgccgtaa taaattgcag
     7441 gtaggcatcg gaaatactgc tcaccccttg ccagatcccc acttcatccc agctatactg
     7501 cgccgccagc agtttacgca tcatgatgta agcaacaggc aaggtcaccg acgtaatcaa
     7561 cgccatgagc gtaaatttgc tcaactgccc tgccagaccg ttatcccagc tgggtttcag
     7621 atagcttaac gggatgacac cacgtttgat caacatgatg gcggcaggaa tcaccaccag
     7681 cgcgggaatc agcgccagac ccagcaacgc cccttcataa ccgcccaaac ggtaactgac
     7741 gtagtacgcg agaacgccaa tcaagctgcc gacaatcagc gataacgcat tacctgcggc
     7801 atcgcgaaag cctttcatca gcgccagtaa caggttgccc caggcgatcc ccatttgcac
     7861 cagcgccact aaacgcacca gcccctgata gtcggtatta ccaaacagtc cctggctgat
     7921 tggcgcagct gccagcacaa aaaccagcgc catcagcgta gagaaaccaa gtaccatcgc
     7981 tgatgaagtg ccgaccacgc ggcgcagctg ttgcggatta tcatggtact gggcaacgta
     8041 tttggttaca ccgttaaaga tgccagctcc ggcaagcacg ccgagcacgg taatcaactg
     8101 gcggaaattt gccgccagcc caagccccgc cggaccaaat gacacagcca gcaacttacc
     8161 gaccagtaac ccggcaccaa tcttgaccag tgtactggcc gccgtccaca aggacgcttt
     8221 tgccaacgac atatcaggag aagtagttca acaaagtcgc aattaccgta cgctgattga
     8281 cgggcgacag gttgtagaac agcggcaggc gcagcaggcg ctcgctctct ttggtggtgt
     8341 agcgatcttc accgtggaac tcaccaaagc gttcccccgc agggcaaccg tgcagcggaa
     8401 tgtaatggaa caccgccatg atttccgctt ctttcagaaa gttaatcaac gcgctccggt
     8461 catcaatatc ccgcagttta atgtagaaca tatgcgcgtt ctgcacgcag ccatcgggaa
     8521 tcgacggcag ctcgatacgc ccggctttcg ccagaggcgc taacgcatcg tagtagtttt
     8581 gccacagcgc cagacgttgc tggttgatac gatccgctgc ttccagttgc gcccacaggt
     8641 acgcagcttg cagatcggac atcaaatagc tggagccaat atcgcgccag gtatatttat
     8701 cgacctgacc acggaagaac tggctgcggt tagtgccctt ttcacggatg atctcggctc
     8761 gttcgattaa cgctttatcg ttaatcagcg tcgcgccgcc ttcaccaccc gccgtatagt
     8821 ttttggtttc atggaagcta aagcagccaa tatgaccaat ggttcccagt gcacgccctt
     8881 tgtaagtgga catcacgccc tgagcggcat cttctaccac aaacagatta tgctttttcg
     8941 ccaacgccat aatggtgtcc atttcgcagg ccacacccgc gtaatggacc ggcacgataa
     9001 cgcgcgtttt gtcggtgatc gccgcttcaa tcagcgtttc gtcgatgttc atggtgtccg
     9061 ggcgaacatc cacaaaaacg atttttgcgc cacgcagcac aaaggcattg gcggtggaga
     9121 caaaggtgta gctcggcatg atcacttcat cgccaggctg gatatcgagc agcagcgccg
     9181 ccatctccag cgaagcggtg caggacggcg tcagtaacac tttggcgctg ccaaaacgtt
     9241 gctccagcca ctgctggcag cgacgggtaa aaccgccatc gccacacagt ttgccgctac
     9301 ccattgccga ctgcatatag tcgagttcgg ttcccaccac cggcggtgcg ttaaatggaa
     9361 tcatgtgatc acctgtataa ccagtacgcg gtgctttcta cattcgcacc actttgtatg
     9421 tatcgtttaa gcgcggcggt gttgcccatt tgggtcgcca cccgcaaagt tgttttaccg
     9481 cgagcatacg cccagtttag cgccgtttgc atcagctcag cacctgcacc gcgtccagcc
     9541 agcaggccaa ttcgcgcatc tgtcgcattg agttcccgta aagagacata accgcgaata
     9601 ccgccggacg ccgcacgtaa aatcagacat tgatgatcaa aggtgccgcg cacggcattt
     9661 tcaatccact gtgcataaaa gcgtccgctg gcgtcaggcg cataccacgg cgcacgaaaa
     9721 cggctttgcg caaatgcggc gctggctaac tgacgtaatg cgggaatatc ggtctcttgt
     9781 gccactacag caccactatc actgacattg ttcacgggta gtgccaaatc aacttcacct
     9841 tctaccaggg agaatcccag ctgttgcagg gcatccagtt cacccgtatt tgatgccgca
     9901 attttggcct gcacccgtga ccacggcgct aacacgtctg gcgtcaggag cggtgcttca
     9961 gacgtaatgc gcacgatggc gctgttaaca ccaaagaagg cgttttccca ggttagtggc
    10021 tcaatactgg cgcggacggg cacgaagtaa ctccagcaga tattggccgt agccagtttt
    10081 cgctaatgaa ctggcagcac gcttcacacc ctcgtcatcg agccagccgt tacgccaggc
    10141 gatctcttcc aggcaggcaa tcttaaagcc ctggcgtttt tctactgtct gcacaaaggt
    10201 actggcttca atcaggctgt cgtgagtgcc agtgtccagc caggcaaatc cgcgcccgag
    10261 cagttcaacg gtcaggttgc ccgcctcgag gtacatctgg ttgatggagg taatctccag
    10321 ttcaccacgc tccgacggct tcacctgctt tgcgtactcc acgactttac tgtcgtagaa
    10381 ataaagcccg gtcaccgccc agtttgactt cggctgtttt ggcttttctt ccagcgagat
    10441 agcgcggaaa ttgtcgtcaa actccaccac gccaaagcgt tccgggtcca tcacctgata
    10501 gccaaatacc gtcgcccctt ccgtgcgcgc cgcaacatga cgcagcttcg gactgaagcc
    10561 ctgaccgaag aagatgttat cgcccagcac cagacaagaa ggttcaccgt tgaggaaggt
    10621 ttcaccgatg ataaaggcct gcgccagacc gtccgggctg ggctgttcgg cgtattccag
    10681 ctggataccg aactcactac catcgcccag caggcgctgg aaataacctt tatcttccgg
    10741 cgtagtgatg atgagaattt cgcggatatc ggccaacatc agcaccgaca gcggatagta
    10801 aatcattggc ttatcgtaaa tcggcaacag ttgcttcgat acgccgcgcg taatcggatg
    10861 caatcgggtg ccggaaccgc ccgccaggat aatacctttc atgtttgcct ccgctgaaaa
    10921 ttagcctttc agacctaaac gctcgccctg atagctgccg tcctgcacct gcttccacca
    10981 gctttcatta gccagatacc actgcaccgt tttacgcatt ccactttcaa aggtttcctg
    11041 tggcagccag ccaagttcac gggcaatttt cgaagcatca atggcataac gcagatcatg
    11101 ccccggacgg tcagcgacaa aggtgatcaa gtcacgatag tgcaccacgc cgtgcggctt
    11161 gttcggagcc agttcttcca gcagctcgca aatggtttcc acaacatcga gattcttacg
    11221 ctcgttgtga ccaccaatat tataggtttc accgactttc ccggtggtcg ccacgcaata
    11281 cagcgcgcgg gcgtgatctt ccacatacag ccagtcacgg atttgctgcc cgttgccata
    11341 taccggcagc ggtttacccg ccagcgcgtt gaggatcatc agcgggatca gtttttccgg
    11401 aaagtggtaa ggaccgtagt tattcgagca gttggtgata agcgtcggca gaccgtaggt
    11461 ccgcagccag gcgcgcacca gatggtcgct gctggctttt gacgcggaat aagggctgct
    11521 cggcgcatac ggcgtggttt cggtgaagaa atcatccgtc gagtgcaggt caccatatac
    11581 ttcgtcggtg gagatatgat gaaaacggaa cgctgatttt ttatcttccg tcagcgtatt
    11641 ccagtaagcc cgcgccgctt caagcaatgt ataagtcccg acaatgttgg tttcaataaa
    11701 cgctgccggg ccgtcaatag aacggtcaac atggctttcg gctgccagat gcatgacaca
    11761 gtctggctga tgctcagtga atacgcgtgc cagttctgcc cgatcgcaga tatcaacttt
    11821 ctcaaaggca aagcgctcgc tttgcgcgac cggtgccagc gacatcaggt ttccggcgta
    11881 ggtcagctta tcgaccacta ccaccgcgtc gctcgtttcg ttgatgatat aacgcaccag
    11941 cgccgagcca ataaacccgg caccacctgt tatcagaatt tttctcatca gcgccagact
    12001 cctttggcat cgacgacata ctgctgatgg acattgtcgc cattgataac tttaaactga
    12061 ctatgatcga ccagcatcac cagcacgtct gccgttgcca gcgcctcgtc aagctgcgcc
    12121 agagtacaaa gcccggtcag ttttttcggc aactggtgga tgttaggctc aacaaccaga
    12181 gtttcgccgc tatgccactg ggcgatcagt tcagcgattt ccatcgccgg gctttcgcgc
    12241 aggtcatcaa tattcggttt aaacgccaga ccaaagcagg cgattttcag ttcactggcg
    12301 cgtttatcgg tagccgccag gcaatcagcc accgccgctt tcacctgatc gataacccag
    12361 aacggtttgt gatcgttcac ttcgcgcgcg gtacggataa gccgcgcctg ctgggggttc
    12421 tgtgccacga taaaccacgg atcaacagca atgcagtgac cgcccacgcc agggccaggc
    12481 tgaagaatat taacgcgagg gtgacgattc gccaggcgaa tcagttccca gacgttaatc
    12541 ccctgatcgg cacaaatcag cgacaattca ttagcaaaag cgatattcac atcgcggaag
    12601 ctgttttcgg tgagcttaca catttccgcc gtccgcgagt tagtgacgac acactcacct
    12661 tcgaggaaaa ttttgtacag ttcgctggcg cgggtcgaac aaaccggcgt cataccacca
    12721 atcacgcgat cgtttttaat cagctcgacc ataacctggc ccggtaacac gcgttccggg
    12781 cagtaagcaa tgttgacgtc cgcctgctcg cccacctgct gtgggaaagt gagatccgga
    12841 cgcatctctg ctaaccattc tgccatcttc tcggttgacc ccaccggcga ggtggattca
    12901 aggatcacca gcgcgccttt tttcagcact ggcgcaatgg agcgagcagc cgattcaacg
    12961 taggtcatat ctggctcatg atcgccttta aacggcgtgg gtacagcaat cagccaggca
    13021 tccgcttcaa ctggcgtcgt gctcgctcgt aaaaaaccgc cttctacggc agtttttact
    13081 acgctcgcca aatcaggctc gacgatatgg atttcgccac gattgatggt atcaaccgcg
    13141 tgttggttga tatcgacacc aatcacctgt ttttgccgtg aggcaaacgc tgctgctgta
    13201 ggcagcccga tgtaacccag tccgataaca gaaatggtcg caaaactcat agtgatatcc
    13261 gattattttt taacgcttcc agaatgcgag agcatgcctg accatcacca tacgggttat
    13321 gggcgcggct catagtttga tattcgtttt cgtcttttaa aagacgcgtc acttcctcga
    13381 caattcgctg cttatccgtg cctaccagac gcaccgtacc cgccgtcacc gcttccggac
    13441 gctcagtggt atcgcgcatc accagcaccg gtttccccag cgaaggcgct tcttcctgaa
    13501 tgccgcctga gtcggtcaaa atcagccagg cgtggttcat cagccagaca aacggtaaat
    13561 actcctgggg atcgatcaga atgacatttt tcacatgccc cagaatgcga ttgaccggct
    13621 ctctgacgtt cgggttgaga tgcaccggat agacaatctg gatgtcctgg tgcgtggtgg
    13681 caatgtctgc caacgcgtgg cagatttctt caaagccacg accgaaactt tcacgcctgt
    13741 gaccggtcac cagaatcatc tttttatcgg ggtcgataaa cgggtaattt gccgccagtt
    13801 ctgaacgcag cgtatcgctg ctcatcacct ggtcacgcac ccataacagt gcatcaataa
    13861 ctgtattacc agtaatgaag attcggctat ccgcaacgtt ttcacgcagc aagttttgcc
    13921 gggaagtttc ggttggagag aagtgataca tcgccagatg cccggtcaat gtacggttag
    13981 cctcttccgg ccacggcgaa taaagatcgc ccgtgcgcag accagcctca acgtgaccaa
    14041 caggaatacg ctgataaaac gccgccaggc tggttgccag cgtcgtcgtt gtgtcgccgt
    14101 gaaccagcac gacgtctggt ttgaactcgg caagaatagg ttttagccct tccagaatcc
    14161 gacaggttat ctctgtcagg ccctgtcctg gctgcattat gttgagatcg tagtcaggta
    14221 caatggaaaa gagtttcagc acctgatcga gcatctcccg atgctgcgca gtgacgcaaa
    14281 ctttagcctc aaaaaaagga tcttttgcca acgcatgcac caacggcgcc atcttgatgg
    14341 cttccgggcg cgtaccaaat acagtcagta ctttcacatc gattctcttc gaataagcgg
    14401 cgagcgcctt tgcgctcacc gcagcagtgt tgctatttcg agcaacggcg ggttaatgcg
    14461 acaccagccc cgatcagccc cccgacaatg ccccacataa tcatcaggaa ggcacgacgt
    14521 gggctatcgc gttttaccgg ttcttccggc gtacgcaaat agcgataggt ctgaaaacgc
    14581 ggatccaggg ttggaccaac attcagggtg tttaacatgg cccgattctg atcatagtcg
    14641 agatcaaagg ccggaccgac ggcctgtaaa ttttccagtc gagcctggag cattggacgc
    14701 ccaagcagga acatttctga atcaggtaat tcctcggcag gcacatctgt cgcactgcgc
    14761 gaaatattat gctgctcagc aattttcagc gcctgttcaa tactgttcat ccggcggtcg
    14821 tagatggctt tcgccacctc ttcctgacgc ttcacctgtg ctttcatctg gatggtacgc
    14881 gccgcccatg cgcctttcag ctcatcattc agatggctgg ctgcacgctg gctggcaaaa
    14941 gcaacatact gacgtaacag gttattagcg tcaggcgcgg tttcagcaat aagcttcacg
    15001 ctgtcattga ccgcgcgggt aaagtctccg gggataaact ggatgttgtt aatcatttca
    15061 tccagcaacg ccgcatcggc tttgctgttg cccaccatcc gctgtttgta atagtcggtt
    15121 tgcagccaga actctctgcg ggtatcccac gaggccagtt gcataacaaa ctctttatag
    15181 gcttcgtcca tgaccgatgg ttggtcggca gaagccatgt ttgaacggac atccaggtta
    15241 cgcaaaaatt gctgctgcga gtaatatccc cccagcatat tcaccgttgg acgatcggta
    15301 atcgccgtcg agctccactc ctgacgagca aaaaaagtat acgccagcgc gattaacgca
    15361 aatgccagcc ccatgccaat aatccatagc ttcccagccc acaaggtacg aaacaaccca
    15421 cgaatatcca gttcattttc agcgtcttcg gccggtttcc caggcattgg ttgtgtcatc
    15481 acatcctcat ttatttggtt aaattggggc tgccaccacg atttctacgc agtctgcgtt
    15541 ttacgcgctt aataaagcga gcaactttcc aggcacgctt aatgcaatat ccatagagga
    15601 agaatgctag caaaaagagc accagcatga cccactccgg gacaaaatga gaatattctg
    15661 ccagcacgcc aatggaagcg agcagtgccg cagcaagggt aatcagcaca aacgcctgac
    15721 gggaagtaaa cccggcacgc atgatcaaat ggtgaatatg ctgacggtca ggagagaatg
    15781 ggctcatgcc tttacgcagg cgacggtaca taatcgccac catatccatt agcggaatgg
    15841 cgattatcca caaagcggta accgggctga tgggatgggt tttgccctgg gtcgtttcga
    15901 gcaggatcca gataacggta aaaccaatca gcgtactgcc cgcatcaccc ataaagactt
    15961 tgtagcggcg acccaggata ccaaggttaa gcatgatgta tggcaggatg gcggcgatca
    16021 tcgcaaagca ccagattgcg aggctggttt gcccgtcgaa ccacaaaatc ataccgattg
    16081 ctgcaaacga gacgcaggac aacccgccca gcaagccatc aatgccatca accatgttga
    16141 acgcattaat ggccgcccag acggcaaata gcgtcaggaa gtaaccaaac ggtccgagca
    16201 ccatctccca ggagccaaag atataaccca ggctactgag ataaagtttg ccgaacacca
    16261 tcataacaat gccaacagcg gcctgtatgg tggcacggat ttttacgctg atatcaaaac
    16321 ggtcatccag cgcgccaatg aaaacaagca caccggcaca agcgagatag agagatgcat
    16381 gcggaatata gtaatcgaca attccgaacg tgaagcaaat ccctgcgtaa accgaaatcc
    16441 ccccaacgag aggtatcaat ccctggtgac gtttgcggaa gtttggttta tccactaaac
    16501 cgactttttt tgccacctta cgggcaaaaa acagaaacag tgtcgtgaat aaaaaaatac
    16561 tgatgagatc agtactcact gtcagtaaat tcacaatgca tgctctcaga gaaaattatt
    16621 agcagaagta taaccacgaa gacctttatt cagaagggaa agtccgttcc gaacccacca
    16681 gtcctgtgca aaaattaacc atttgatatg caaattgcat aacacgccag caaattccag
    16741 gaattttcct aacatcgcca gcgcggcatt aagattacag atataaaagc aaaacgccac
    16801 gtaaacacgt ggcgtttttg gcataagaca aatttatgag cgtttcatca tttcgaagaa
    16861 atcgtcattg gtcttggtca tggccagttt attaatgagg aattccattg catcgatttc
    16921 gcccatcggg tgaatgattt tgcgcaggat ccacattttc tgcagttctt cctgagtagt
    16981 gagcagttct tctttacggg taccggaacg gttgtagtcg atagccggga agacgcgttt
    17041 ttcagcgatc ttacgagaga ggtgcagttc catgttgcct gtacctttaa actcttcgta
    17101 gataacttcg tccattttag aaccggtatc gataagcgcc gtcgcgataa tagtcaggct
    17161 gccgccctct tccacgttac gcgccgcacc aaagaagcgt ttcggacgat gcagggcgtt
    17221 ggcatccaca ccaccggtca acactttacc tgacgccgga acaacggtgt tgtaagcgcg
    17281 cgccagacga gtgatggagt cgagcagaat gataacgtct ttcttgtgct caaccaggcg
    17341 tttggccttc tcgatcacca tttccgcaac ctgaacgtgg cgagatgcgg gttcgtcaaa
    17401 ggtagaagca acaacttcac cttttaccag acgctgcatc tcggttactt cttccggacg
    17461 ttcgtcgatc agcagaacca tcagcacaca atccgggtgg ttgtaagcaa tgctctgggc
    17521 aatgttctgc agcagcatgg ttttaccggc tttcggcggt gccacaatca gaccacgctg
    17581 accacgaccg ataggtgatg ccagatccag tacgcgagca gttaaatctt cagtagaacc
    17641 gttaccacgt tccatacgca gacgagaatt tgcgtgcagc ggggttaagt tctcaaagag
    17701 gattttgttg cgggcgtttt caggtttgtc gaagttaact tcgttaactt tcagcagcgc
    17761 aaaatagcgt tcaccttctt tcggcgggcg aatcttacca gagatggtat caccagtgcg
    17821 gaggttgaaa cggcggattt ggctagggga aacgtagatg tcatcaggac cggcgaggta
    17881 ggagctgtct gcggaacgga ggaaaccaaa tccatcctgc aatatctcca gtacgccatc
    17941 accaaagata tcttcgccac tctttgcgtg ctgcttcagg atggcaaaaa taatgtcctg
    18001 cttacgcatg cgagccaggt tttccagccc catattttcg ccgagagtga tcagctcaga
    18061 aaccggcgta ttctttaatt cggtaagatt catagtggtg tgagttctta aacttggggt
    18121 aaatctcgaa cttaatgttg tgaatggtat ggcaggatca tccatgcctg tttacggtca
    18181 tcgactcatg tcttttcgct gcctggtcac aggagagtac gcagaactga aacgacaaga
    18241 cggattgagt gacgagccag aaatctgttc acttcgcatt taagataaaa cgggaagcgt
    18301 tggaaattac aagattcaaa cttaataagg tatgtttaat acgaagtcaa cactaagtta
    18361 gcatgactca cgccgggcgt ccagttttta gcgacggggc acccgaacat gaaattccct
    18421 tacgccaggt tagcgtcgag gaactctttc aactgacctt tagacagtgc gcccactttg
    18481 gttgccgcca cttcaccgtt tttgaacagc agcagagtcg ggataccacg gatgccatat
    18541 ttcggcgcag tgccagggtt ttgatcgatg ttcagttttg caacggtcag tttgccctga
    18601 tattcgtcag cgatttcatc cagaatcggg gcgatcattt tgcacggacc gcaccactct
    18661 gcccagaaat cgacgaggat cgccccgtcc gctttgagta catccgtgtc aaaactgtcg
    18721 tcagtcaggt gaataatttt atcgctcata tataactcca caggaataag cctggcgtgt
    18781 tggtttcgtt gttggtgtaa cattaaccaa ctaaaggttg actttatttc accggatacg
    18841 ctttcgtaaa gcaatagtaa gctgatattc taccacacta tgagcaaaac acatttaaca
    18901 gaacagaagt tttccgactt cgccctgcat ccgaaggttg tagaagccct tgaaaaaaaa
    18961 gggtttcata actgtacgcc cattcaggca ctggcccttc cgctgacgct ggcgggtcgt
    19021 gacgtagccg ggcaggcgca aaccggtacc gggaaaacga tggcgtttct tacgtcaacg
    19081 tttcattatc ttctctctca tcctgcgatt gccgatcgca aggtgaatca accgcgtgcc
    19141 ttaattatgg caccgacgcg tgaacttgcc gtgcagatcc atgccgacgc agaaccgctg
    19201 gcggaagcta ctggcctgaa gctgggtctg gcttacggtg gtgatggcta cgacaaacag
    19261 ctgaaagtgc tggaaagcgg cgttgacatt ctgattggca ccacgggtcg tttaattgac
    19321 tacgccaagc agaaccacat taacctcggt gccattcagg tagtggttct ggatgaagcc
    19381 gatcgcatgt acgatctggg ctttattaaa gatatccgct ggctgttccg ccgtatgccg
    19441 cctgcaaacc agcgcctcaa catgctgttc tccgccacgc tttcgtaccg ggtacgtgaa
    19501 ctggcgttcg agcagatgaa caatgccgaa tatattgaag tggaaccgga acagaaaacg
    19561 ggccaccgta ttaaagaaga gcttttctac ccttctaacg aagaaaaaat gcgtttgctg
    19621 caaacgctga tcgaagaaga gtggccagac agagcgatta ttttcgccaa taccaaacac
    19681 cgttgtgaag agatctgggg ccacctggcg gcagatggtc atcgtgtcgg tttgttgaca
    19741 ggcgatgtcg cgcagaaaaa acgtctgcgt attcttgatg aatttacccg tggcgatctg
    19801 gatattctgg ttgccaccga cgttgccgcg cgtggtttgc atattccggc agtgacgcac
    19861 gtctttaact acgatttacc cgatgactgt gaagattacg ttcaccgtat tggtcgtaca
    19921 ggtcgcgcag gcgcaagcgg tcactctatc agcctggcgt gtgaagagta tgcattgaat
    19981 ttgcctgcta ttgaaaccta tattggtcac tcaattccgg taagcaaata caatccggac
    20041 gcattgatga ccgatctgcc aaaaccgctg cgcctcacgc gcccacgtac aggcaatggt
    20101 ccgcgtcgta ctggcgctcc gcgtaatcgt cgtcgttcag gttaaataaa atgtcatact
    20161 caaaatcatt caaactgtat caagatggca agagaatgaa tcccgatgag catactgtag
    20221 taagtgattc aggtgaaaga atgcagccaa cacagagaca gattgaagga tgaagagtat
    20281 gggttccacc tcgtcgctgt atgcagccat tgatctcggt tcgaatagtt ttcatatgct
    20341 ggttgtgcgc gaggtggctg gaagcatcca gacgctgacg cgaataaaac gcaaagtgcg
    20401 tctggctgct ggcctgaata gcgaaaatgc cctgtctaat gaagcaatgg agcgcggttg
    20461 gcaatgtctg cgcctgtttg ctgaacgtct gcaagatatc cctccctcgc aaattcgcgt
    20521 tgtcgctacg gcgacattac gtcttgccgt caatgcgggt gattttattg ccaatgcgca
    20581 ggaaatcctc ggttgtccgg tacaggtgat cagcggtgaa gaggaagcac gtctgattta
    20641 tcagggcgtt gctcacacaa ctggcggtgc cgatcagcgc ctggtggtgg atataggcgg
    20701 tgccagtact gaactggtaa ccggcacggg tgcacaaacc acctcgttgt tcagcctgtc
    20761 gatgggctgc gtcacctggc tggaacgcta ttttgccgat cgtaatctgg ggcaggaaaa
    20821 ttttgatgct gcagaaaaag cggcacgcga agtgttacgt ccggttgccg atgaattacg
    20881 gtatcacggc tggaaagtgt gcgttggtgc ttccggcacc gtgcaggcgt tacaggaaat
    20941 catgatggca caggggatgg atgaacgcat taccctggaa aagttgcagc aattgaaaca
    21001 gcgagccatt cattgcggtc ggctggagga actggagatt gacgggctga cgctggaacg
    21061 tgcgttagtg ttcccgagtg gtctggcgat cctgatcgcc atttttaccg aactgaatat
    21121 tcagtgtatg accctggcgg gcggtgcgct gcgtgaaggc ctggtctacg gcatgttgca
    21181 tctggccgtc gagcaggata ttcgcagccg tacgctgcgt aatattcagc gccgctttat
    21241 gatcgatatt gatcaggcgc agcgcgtagc caaagttgcg gctaacttct tcgatcaggt
    21301 ggaaaaggaa tggcatcttg aagcaataag ccgcgatttg ctcatcagcg cctgtcagct
    21361 tcatgaaatc ggcctgagcg ttgactttaa acaagcgccg caacacgctg cttatctggt
    21421 gcgtaatctg gatcttcccg gttttacccc cgcacagaaa aaattgctgg cgacgctact
    21481 gctcaaccag actaatccgg tcgatctctc atcgctgcat cagcaaaatg ccgtaccgcc
    21541 gcgcgtcgca gaacaactct gccgtttact ccgcctggcc atcatttttg ccagccgtcg
    21601 ccgtgacgat ctcgtgccag agatgacatt acaggctaac catgaactgt tgaccttgac
    21661 gcttccgcaa ggttggctaa cccaacatcc gctgggtaaa gagattattg ctcaggaaag
    21721 ccagtggcag agctatgtcc actggccgct ggaagtgcat taatgagtaa gtgccggatg
    21781 cgatgctgac gcatcttttc cggccttgat tatttccctc gttttgccgc catcatcgct
    21841 ttcagattcg ccagatggct ttgccctttc tgcatccgtt cttcggcgct gaccactttg
    21901 cgctcctgtt cccaaatcag atcatcctgc ggcagctcca gcaaaaagcg gctcggctcc
    21961 gggcgcacca gctcgccgta ctgacggcgt tctttacaca gcgtaaaggt caattccttc
    22021 tgggcgcggg taatgccgac ataggccagc cgccgctcct catcgatatt atcttcatcg
    22081 atgctgctct ggtgcggcaa aaacccttct tccataccga ccatgtagac ataaggaaac
    22141 tccagccctt ttgacgcgtg cagagtcatc agttgcacct gatccagctc ttcttcactc
    22201 tcaccacgct ccatcatgtc gcgcaaagta aagcgcgtca ccacctgggt gagcgtcatc
    22261 ggctcatcca gttcgctgcc ttccagcatc tccgtcatcc agctaaacag ttggttgacg
    22321 ttcttcatgc gcatttcggc ggctttcggg ctgggcgatg tttcgtacag ccaggattca
    22381 taatccatgc cgtggatcag atcgcgcacc gcggcaatcg gctcccgctc cgccagacgc
    22441 tggatttctg ccaaccagtg agtaaagcgg gtcaatgctt cataaccacg tccgctaagc
    22501 gtctgactca ggcccatatc aaagctggcg gtaaacatgc ttttattgcg cgtcatcgcc
    22561 cactcaccca gctttttcag cgtcgccggg ccaatctctc tcttcggcgt gttaacgata
    22621 cgcagaaatg cgctgtcatc gtccgggtta gtcagcacgc gcagataagc cagcaagtcc
    22681 ttgatttcag gacgagagaa aaacgacgta ccaccagaaa ttttgtacgg gatgcggttt
    22741 tgcatcagga atttttcaaa cactcgcgac tgatggttac cgcgataaag aatagcgtaa
    22801 tctttgtact gcgttttatt gacgaagtga tgcgcaatca gttcaccagt gacgcgctcg
    22861 gcttcatgtt cttcgttatt cgcgcttaaa actttaagct ctgtgccgta acccagttcg
    22921 gagaacagac gcttctcaaa gacgtgcggg ttattggcga tcaggatgtt cgccgctttc
    22981 aaaatacgtc cggaagagcg gtagttctgc tcaagcttaa tcaccttcag cgccggaaaa
    23041 tcctgactca gcagcaccag gttttgtgga cgtgcgccgc gccaggagta gatcgactgg
    23101 tcgtcatcgc ccaccacggt aaagcgcgcg cggctgccca ccagcagttt caccagttca
    23161 tactggctgg tgttggtgtc ctgatactca tccaccagca gatagcgaat tttgttctgc
    23221 cagcgctcgc ggacttcttc attgcgttgc agcagcaacg tcggcaataa aatcagatca
    23281 tcgaagtcga gaacattgca cgctttcagg tgtgcatcat acagcccata acaatgggca
    23341 aaaatacggt cccgctcgcc aatcgcactg gctgccgcct gggacggtgt tttgagatcg
    23401 ttcttccagt tagagatggt cgaaatcaac tgttgcagga gaactttgtc atcttcaatc
    23461 agcccttcgg tcaactcttt gagcaacgcc agctgatcgg tatcgtcaaa cagcgaaaag
    23521 ttcgccttca tccccagcgc cgcatactca cgcttgatga tatccagccc cagcgtgtgg
    23581 aaggttgaga tcatcagccc acgcgcctct ttgcgaccca gcgtctgtcc gacacgctct
    23641 ttcatctcgc gcgccgcctt attggtaaag gtcaccgccg cgatatgtcg cgcctgataa
    23701 ccgcaaccgc ggatcagatg ggcgatttta ttggtgatca cacgagtttt accggaaccc
    23761 gcgcccgcca gcaccaggca ggggccggta acgaattcga cagcttgttg ttggccgggg
    23821 tttagacgca taggtgtatt gctcaatctt cgaacggggg gaggattgta gcagaatcgc
    23881 atttgaatgt gaccgcgtgt aagccgctaa ccgattggct gcggatgttt aaatcagaga
    23941 gggaagaccg aaaccggagt tcatcgtgaa tccattagac ttagaaaata tcgggtcgta
    24001 acgttgtgtt attacttttt cagagtttac taaggagagg tgtactcgtg gcgattaatt
    24061 tcagtcctaa ggttggtgaa atactggatt gcaactttgg taactatcct gttagccaga
    24121 atggacagtt cagcaccacg tattacgatg gccgcattcc tcctgaaatg ataaaaaaca
    24181 ggttagt
//
LOCUS       DAZPEP010000048        23719 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZPEP010000048 DAZPEP010000000
VERSION     DAZPEP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23719
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.048"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..308)
                     /locus_tag="ACHUT9_001917"
     CDS             complement(<1..308)
                     /locus_tag="ACHUT9_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1348399.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     "
     gene            complement(305..511)
                     /locus_tag="ACHUT9_001918"
     CDS             complement(305..511)
                     /locus_tag="ACHUT9_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR1348400.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            complement(508..2433)
                     /locus_tag="ACHUT9_001919"
     CDS             complement(508..2433)
                     /locus_tag="ACHUT9_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1348401.1"
                     /translation="MNISNSQVDRLRHFVRAGLRALFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWSPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEEGAAT
                     NKKTLADYARALSGEDE"
     gene            complement(2408..2953)
                     /locus_tag="ACHUT9_001920"
     CDS             complement(2408..2953)
                     /locus_tag="ACHUT9_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR1348402.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRLASEADLHPGTLEFERHRLTRAQATAQELKNA
                     KESAEVVETAFCTFVLSRIAREISSILDGIPLSVQRRFPELDNRHIDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3243..3419
                     /locus_tag="ACHUT9_001921"
     CDS             3243..3419
                     /locus_tag="ACHUT9_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348403.1"
                     /translation="MRDKTAHHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLMREYSD
                     DTAHDVSGLNTFIR"
     gene            complement(3501..3950)
                     /locus_tag="ACHUT9_001922"
     CDS             complement(3501..3950)
                     /locus_tag="ACHUT9_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348404.1"
                     /translation="MQEEANKILVDLLKKASDGIDSAIAFSQAQIPDVVHQLLVWNMV
                     DSLIKTLIAISTIPLVIWFMKKQCKKVEIGKFDNEGRSCDNGQPKYKPTMIWESDGRL
                     SGFVLPLVAVFILWFSFIISVVANMTWLKIWLAPKLYLIEYAASLIK"
     gene            complement(4074..4934)
                     /locus_tag="ACHUT9_001923"
     CDS             complement(4074..4934)
                     /locus_tag="ACHUT9_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348405.1"
                     /translation="MKELQKINSIMERLSALERKLDDCGKLCVEKQNELYREFFTIVS
                     ELMPIVNNCLMTSRFFNVDATTSRKFSVKIQERVSGSVTREMAEQLVAMSNKTKRKKA
                     LLVTANQLSGRVDFQVRQGGRVLVNDAFVGRSFSPYSMKYDVDADDQDISISWKSETA
                     GIVLTAELLSDDKNDVQHHLDEKNHISEICMKPRSGMTLLSRTLAAGFSAPEKRVLLV
                     VPEDADIRPLNGCDVISAKTLHTRVIPVPDVVIIDDVEKCKSVSSTATIKRILRCSTS
                     AVVFRKQLHN"
     gene            complement(5215..5526)
                     /locus_tag="ACHUT9_001924"
     CDS             complement(5215..5526)
                     /locus_tag="ACHUT9_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFR1348406.1"
                     /translation="MKIYIAGPMTGYENFNRDAFNKEADRLSRHGHSVLNPATLPNGL
                     TQREYMDICFAMLRCADAILMLPGWKASAGATAEYHYAYKMEMPVFTTLNYPPACSSV
                     A"
     gene            complement(5568..6005)
                     /locus_tag="ACHUT9_001925"
     CDS             complement(5568..6005)
                     /locus_tag="ACHUT9_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1348407.1"
                     /translation="MSRVTAIISGLVICIIICLSWAVNHYRDNAIAYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GNGESPRLTADAELNYYRLRSGIDRITAQVNYLQEYIKTQCLN"
     gene            complement(6002..6499)
                     /gene="rrrD"
                     /locus_tag="ACHUT9_001926"
     CDS             complement(6002..6499)
                     /gene="rrrD"
                     /locus_tag="ACHUT9_001926"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFR1348408.1"
                     /translation="MQSSLRKAVTAAIGGGAIAIASMLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCYGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWISAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(6499..6771)
                     /locus_tag="ACHUT9_001927"
     CDS             complement(6499..6771)
                     /locus_tag="ACHUT9_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348409.1"
                     /translation="MKMDERYSSASYGSAGLAAFFASLSLQDWGFIIGVAFSIILGVL
                     TYRLNKREQMKRTKILQDILDKTNTNNLSATAMVIGELGKRAPKEI"
     gene            complement(6867..7943)
                     /locus_tag="ACHUT9_001928"
     CDS             complement(6867..7943)
                     /locus_tag="ACHUT9_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1348410.1"
                     /translation="MFKTTNIHGAQLVCADSLQFIKTIPDNSVNLIATDPPYFGVKAN
                     AWDNQWDSDADFLGWIDEFLAEFWRILAPNGSLYMFTGSRLASKIELLTRDRFNVLNH
                     IIWAKPSGMWRGCHKESLRAYFPATERILFAEHYGASGYAKGQSGYASKCADLRKNIF
                     SPLIDAFSLARRQLDISAADINSATGKQMCSHWFSYSQWRLPSLIDFNKLCELFRRKA
                     DSLGVPCPYPFNVDYSAHEKRYSDLKLHYEEVKKQYDDLKAQYENLRRPFHVTADVPY
                     TDVWEFPPVQYYPGKHPCEKPAAMMEHIIKSSSRPGDIVADFFMGSGSTIKAALKLGR
                     QAIGIEIESERYLQTVDEVKKLFE"
     gene            complement(8211..8438)
                     /locus_tag="ACHUT9_001929"
     CDS             complement(8211..8438)
                     /locus_tag="ACHUT9_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348411.1"
                     /translation="MNINEIRGNMTEAALSVECVMRGYPRISLKELSEACFLSQAAVE
                     FIIEQMICFGVAKRSGSGRYSLTDEYKQATF"
     gene            complement(8452..8673)
                     /locus_tag="ACHUT9_001930"
     CDS             complement(8452..8673)
                     /locus_tag="ACHUT9_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348412.1"
                     /translation="MNYQGNEKMRDDVAEIANELYELWQKVERFEKEYGFNSTNLADR
                     LAGRLVGTMGPKLAELNRFMADVDFQFQD"
     gene            8838..9149
                     /locus_tag="ACHUT9_001931"
     CDS             8838..9149
                     /locus_tag="ACHUT9_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1348413.1"
                     /translation="MFNVITHPAALDELLELPDDLRGRMTRLIERLEKEGNKLKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPVKAIEIARTRLKEM
                     N"
     gene            9149..9436
                     /locus_tag="ACHUT9_001932"
     CDS             9149..9436
                     /locus_tag="ACHUT9_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1348414.1"
                     /translation="MKAKGIPFNEVKEKLLNTPEAVKGYEEADKELEMVEMLYEMRER
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLAKYAHACGATIDIRAVY"
     gene            complement(9479..9682)
                     /locus_tag="ACHUT9_001933"
     CDS             complement(9479..9682)
                     /locus_tag="ACHUT9_001933"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348415.1"
                     /translation="MSEAAVSTLEQLDSNLRDIDAVLDLVSVTLASPEASLHIGEVSR
                     LINMSREIAQHCQKTIAVERSHH"
     gene            complement(9765..10007)
                     /locus_tag="ACHUT9_001934"
     CDS             complement(9765..10007)
                     /locus_tag="ACHUT9_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348416.1"
                     /translation="MDKKALLFEMIRKRSEKSFSDGGDGFVFASMLAFDVGLNSRIVK
                     RMLDSAVRTGLLEKVDRGIGREHKYRSTQQFTQLIH"
     gene            complement(10368..10754)
                     /locus_tag="ACHUT9_001935"
                     /pseudo
     CDS             complement(10368..10754)
                     /locus_tag="ACHUT9_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829410.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10895..11116)
                     /locus_tag="ACHUT9_001936"
     CDS             complement(10895..11116)
                     /locus_tag="ACHUT9_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020805071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348417.1"
                     /translation="MNKAELFQKISALATECHAIASELDVGDERTEMFEIYSVLRNLC
                     RRGYATQVGRMTNPLLSSCDEDDSDEDDE"
     gene            complement(11109..11843)
                     /locus_tag="ACHUT9_001937"
     CDS             complement(11109..11843)
                     /locus_tag="ACHUT9_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1348418.1"
                     /translation="MKFSKFSELVNRILSNNHSHRRDMDVTIVVHSPGSIGSTPSVEV
                     QSIHAGFDWDSGKVLIFPSQPLTTLTPEQITDITDSVRKGQSWHAYQEYKNHKEQLEK
                     LSIELESAKNHIAELEAKPVSQTYKLTFEQWLEQQHDKIDVDCGCVSTEMLMHWIRVA
                     YEAGNYPVTPDGWISCSERMPEKGQNVLISVNFDSSLVEPLICSARYTGSTFRRGDAT
                     IKPGNGIEQATHWMPLPEPPQESKSE"
     gene            complement(11840..12130)
                     /locus_tag="ACHUT9_001938"
     CDS             complement(11840..12130)
                     /locus_tag="ACHUT9_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFR1348419.1"
                     /translation="MNIDTTITIDTALNTGLALLGWLYIMSRTWRWLGSIFLKQWKKR
                     RKQELRQKALEAFYDAFELSRIEPGTTARIATKGDLMIVMFRQERAEKGESA"
     gene            complement(12130..12549)
                     /locus_tag="ACHUT9_001939"
     CDS             complement(12130..12549)
                     /locus_tag="ACHUT9_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1348420.1"
                     /translation="MAKAFTPEQREELNKQIVELVRLNGRGTVRQLADETGISRCAVS
                     RLSRELAASGDLYISGSGIFLSAQARKDWQNARKKLSRVKPKTSVVVDPDLIWSLPDG
                     EIRRYDRRLNMICHDCRKSEVMQRVLAFYQGNFQEVA"
     gene            complement(12549..13106)
                     /locus_tag="ACHUT9_001940"
     CDS             complement(12549..13106)
                     /locus_tag="ACHUT9_001940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFR1348421.1"
                     /translation="MIDLETMGTNTCAPIVSIGAVFFSPESEELGPTFYVPVNLRSSM
                     LNGGVADGDTILWWLKQSKEARAAICTNDALDIKDALFELSHFITCHACNLKKLKVWG
                     NGATFDNVILRGAYERVGLACPWEYFNDQDVRTIVNLGQFIGFNPKKDMPFDGERHNA
                     LADAIHQAKYVSAIFSRLVKGQGES"
     gene            complement(13172..13345)
                     /locus_tag="ACHUT9_001941"
     CDS             complement(13172..13345)
                     /locus_tag="ACHUT9_001941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348422.1"
                     /translation="MTHDEAERLSDTYRRRGKKVLVVRSDFLGDGYCVYVHLPELERT
                     PKPSRTYQQKIWV"
     gene            complement(13609..14319)
                     /locus_tag="ACHUT9_001942"
     CDS             complement(13609..14319)
                     /locus_tag="ACHUT9_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFR1348423.1"
                     /translation="MELEYIRSCVSTALADVHYRQRGILEVQLEQMRLGRSGRFNNKP
                     VRSISVGDDNSYEVSVPAEPVRFHQGKTFKQSSMLLTDIDFQSASWRRAIGQLNNEES
                     AWLYYCYGCKPDYNNDVIVCQWLWLDFLVAHSGGGFKKMKASTKKAMRKLIYYAAQQV
                     KSELTCAEAVDEREQDRHLSFLLNISIDSWRQDYKERWLLIKSRCLSLNRTALLNAAE
                     KRSEIIKRHRAGSAILPL"
     gene            complement(14309..14530)
                     /locus_tag="ACHUT9_001943"
     CDS             complement(14309..14530)
                     /locus_tag="ACHUT9_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1348424.1"
                     /translation="MKPEELIRHFGSVEKAAAGVGVTPGAVYQWLTAGEIPSLRQSDI
                     EVRTAYKLKSDFTTRRVKQSIKESRKRGA"
     gene            14622..15218
                     /locus_tag="ACHUT9_001944"
     CDS             14622..15218
                     /locus_tag="ACHUT9_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1348425.1"
                     /translation="MKTRGERLKARRLEMKLTLREVAEAVDISIAGVQNLERGDVMPS
                     LEIGIALAKCLRKPVHWVLYGTEYDPDRIPVIGTTESGPDEEWKPGQVPNTERFLPFI
                     SQRNTVYALTISNLTQGNYQPGDFLLVDSALELVPGEDVLVRDTAGNITIQRLARFDD
                     ENYYLDGTNSQRAIFRKSDLEFVHQIVGTIKSFMVEGR"
     gene            15464..19459
                     /locus_tag="ACHUT9_001945"
     CDS             15464..19459
                     /locus_tag="ACHUT9_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication protein, CyRepA1 family"
                     /protein_id="HFR1348426.1"
                     /translation="MTLNDFYADRFGSDPFSLIEAARDELTELAQMAGINWPACSDNI
                     QLNPRGGVERYSSYNKTSPEALEKSLKGRVEIYSRLEHSKGIDYPFINFVHKGSDAGS
                     WSGFSFLFSEYRREQQRNNATVVAQPEEERERIERQAEARRRREEQKRINDLKNNQMD
                     HERLLGWLAFHSAWEHAPAEDGSWPYAIKKGIRDVFSSCDVRRVTSHDSAKWSRGPTT
                     YMAIPLAHLDGRYDGRIVGWQRIDKQGGKYQTSAVTSGDFVGACFVIGELKGAQNIAV
                     TEGFATGASIWLATKKDKRFDAVVVAVSANNMVHVVEQLVNMYPAAKITCALDNDRKS
                     ATEGKGNTGLRTGYEILSKFNDVRCVYPTFEDDPQMECSDFNDLHKLRGLRETARQLF
                     ATANRLKTGTDLLSLTLNRLKTIKRDNRRTFAKELLRAVDIGMLTCPVPNSPSDLFNM
                     FCIVLREMELENIYRATVKDHIARRLNKKCRIAQAPRSFSERITDPNKRPQHITYKRF
                     ETSVMTDDVLKYVQSLQGIVIVRAGMGSGKSTGLLRPLMHSSERGISVAHRVSLIGGL
                     WEMMTEGKGVKADILHYQDPGYQEMAPYASKLTICINSIVKNCWQPLMRQHDYFGFDE
                     ATQGLRAVLSGRAMENPVAVFNTLIDALARTEQHPIMVDADANDLLVDLAELAMKRRE
                     ELGLPAWLQIHVIELPVDVRNRETGEPIRVFYTEKDRIMTEVVKAVSDGEKIMLATDS
                     STFAEDVTATLRMHFPGKKFLCVNQKNKPEPEVEAFTNQPKKMVKKYDGLIYSPSISS
                     GVSIEQKHFDRHFGMFCGEVVPSDAVQMLRRDRTAKEFIIGFDKARAKRETDPQKIER
                     AFAQALLATAGMNGELTDVVFDGDRISMGVANTDFTRMKIKAAAIEASARNDYASNMI
                     CIMHSDGYKVSPLATDYAANDIGKDLRKEARDIVWEQTLDLHLNTETPQESEREELLK
                     KRALTLEEQAKLVRWDIENELKLPVEEGTLKFYFDGARDKVRRYETMLLDEVTARRYD
                     REEAAINFTYAYRQAGQWQYFVVTAMTREQADDVFKTKFPGIIDHKVKATPTVEVGAR
                     GFYALKSATLRQYFIDCGINPETMTGEATQERLKFARDNLMTAERRDLLNNVLHIGGF
                     MTPKGKPKVPEALFKNICDSLGLKTGKRRGRDGDKRPTLRFVEPESAAFMLDILVQRQ
                     KDGLTLKTRKAEKTAIEMDRDLDLNIYMNDKTRSTNEQVQNAPHSVISEALAVLPVAV
                     PESWAMSALSSEELATMSSWSSASIAMTFASLYLTEFMDHLSSNELRRLREYITGNSS
                     CSYEEQGAFYG"
     gene            19648..19974
                     /locus_tag="ACHUT9_001946"
     CDS             19648..19974
                     /locus_tag="ACHUT9_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348427.1"
                     /translation="MSTTAFLDKLDYRQLQFCRDECERRIRAIQEEEKKVAWAVTDRG
                     LIFGWFRTEDYLKAVELLARKAAERWERADKENPETKYELNLYIEGNRLPLSEYNALF
                     ADGEWG"
     gene            19977..20372
                     /locus_tag="ACHUT9_001947"
     CDS             19977..20372
                     /locus_tag="ACHUT9_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348428.1"
                     /translation="MAVQSYKYESPLDKGYIRIKLSRSQHKAIFKVRKIRILDAYAYY
                     YNGENVVVEHFLARWFVALIFVPLLLIGTFVDGFPSTWREIKKGLFPHKYGRFSQDSW
                     RVIPGKHSVDEQPIVDSWLNRMADAKKVD"
     gene            20400..20687
                     /locus_tag="ACHUT9_001948"
     CDS             20400..20687
                     /locus_tag="ACHUT9_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348429.1"
                     /translation="MKSFIFVLCFAFSAAVYAQEDSISVINSSAELACKNNPDAASKE
                     SCKELLLSTAAFASENAKFYSAFCRGYVKDEDKEKCDDAGKLVRYFQSLNQ"
     gene            21064..21228
                     /locus_tag="ACHUT9_001949"
     CDS             21064..21228
                     /locus_tag="ACHUT9_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFR1348430.1"
                     /translation="MFKFKFAAIVRTDKKSHIHHLSTIASSEREARRQFASRFVLVLS
                     ARIPVREVAA"
     gene            21225..21458
                     /locus_tag="ACHUT9_001950"
     CDS             21225..21458
                     /locus_tag="ACHUT9_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348431.1"
                     /translation="MNQVKMNTVGLLESLDERLAQVYALVSSAHRTISSCEASIYLSE
                     AGKLLQIACELTHEAQGYSASLSSQLLHDKEAE"
     gene            21455..21619
                     /locus_tag="ACHUT9_001951"
     CDS             21455..21619
                     /locus_tag="ACHUT9_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFR1348432.1"
                     /translation="MSALAVFSFQEEHQVRVVMINGEPWFVASDVCMAAGIDSTAIRK
                     LDEDEKGQAR"
     gene            complement(21665..23560)
                     /locus_tag="ACHUT9_001952"
     CDS             complement(21665..23560)
                     /locus_tag="ACHUT9_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protelomerase family protein"
                     /protein_id="HFR1348433.1"
                     /translation="MSKVKIGELINSLVSDVESIDNSDRPQGEKTKKIKAVALKFKNA
                     LFNDKRKFRGKGLEKRITANTFNAYMSRARKRFDDKLHHSFEKNIHRLSEKYPLYNEE
                     LLSWLSLPAADIRQRMSALQAKLKLILPLSEDLSNIRIGDNKSHNKLLKLSNKYKEWQ
                     FAISDLTSDNWKDKRDYLYKLLQQGSALLEELNQLKVNHEILYHLQLSPAERASIQQR
                     WADVLHNKKRNVVVIDYPKYMQAIYDIINSPATLNNLNTRSGMAPLAFALAALSGRRM
                     IEIMYQGTFTVSGKYTVDFLGQAKKRTSDDITRKIYTLCEAKTFVYLINVLRSCPAAS
                     DFDDVVKGYGDNDTRSENGRINAILAKAFNPWVKSFFKDDRRVYKDSRAIYARIAYEM
                     FFRVDPRWKNVDEDVFFMEILGHDDENTQLHYKQFKLANFSKTWRPNTGNENTRLEAL
                     QQLDDEMPGFARGDAGVRLHNTVKQLVEQDPSIKITNSTLRAFKFSPTMISRYLEFAA
                     DALGQFVGENGQWQLKIDSPEIVMPDEEELEAIPEQTDELSEDDDLDDDEIEIEEEEE
                     EESVEEQQPPAPSSPVSKPVFKPVRNIGDGSYVVEFHYNGQNYAWSGSANSPISAMQL
                     AWLTYFG"
BASE COUNT         6395 a         5710 c         5167 g         6447 t
ORIGIN      
        1 tcttcctccc cgatgcccag atagcgccag cttgggcgat gactgagccg gaaaaaagac
       61 ccgacgatat gatcctgatg cagctggatg gcgttggcgg catagccgtt attgcgtacc
      121 agatcgtctg cgcgggcatt gccacgggta aagttgggca gcagggctgc atccacactt
      181 tcacccggtg ggttccacgc ccgcaactgc ccaccaaatc cgctgccacc gccgtgataa
      241 ccggcatatt cacgcagcga tgtcatgccg tccggcccca gaagggtggg aatggtggac
      301 gttttcatac ataaaatcct gcaggtcccc tgcgtcgctg tgtcatgccg gtctgcactt
      361 ccagctccgc aatgtatttt ttcaggtcag acacggaagt ggccgtaaac tccactcgcc
      421 gtccgtcttt ctgtaccgtt gccacccgtt tacctgtcat caggtcatgc agtgccgcac
      481 gggcagcggc aagttcttcc tgtcgcgtca ttcatcctct ccggataagg cacgggcgta
      541 atctgccagt gttttcttgt tggttgctgc accctcctct tcctgcaggc tcgccagcag
      601 tgcactgaga tccagctgcc agcgggaaat actgatgcgc agcgccgcca gcgcataaac
      661 gaagcagtcg agcgcctcat tgcgtcgctt tttgctgtcc cacagtattt tttttctgcc
      721 atccacccat ttttcgacct gctcttcagc agtcagctgc tgcgcttcgg tcagatcaaa
      781 aatatccggg ttattcggga agtgaacggc accgggaagc ggttcatccc cttccggcgt
      841 cagtgtgaag cggttataaa tttgctcttt cgcggtatcc gtaccgattt cggtaaggta
      901 aaccccgttt ttgtttcgct tacgtggcat gctggccacc ggctttccgt atacggatgc
      961 acctttaatg gggatcaccc tgaacagccc atgttttttc gagcgttcat acacaatggt
     1021 cgggtcaatc ccgccagtat cccagcagat acgggatacc gacatttctg caccattccg
     1081 gcgggtgtag gttttattga tggcctcatc cacacgcagt agcgtctgct cgtcgtcgtg
     1141 gcggcccata ataatctgcc ggtcaatcag ccagctttcc tcacccggcc cccatcccca
     1201 tacgcgcatt tcgtagcggt ccagctggga gtcgataccg gcggtcaggt aagccacacg
     1261 gtcaggaacg ggcgctgaat aatgctcttt ccgttctgcc atcacttcag catccggacg
     1321 ttcgccgatt ttcgcctccc acgtctcacc gagcgtggtg ttcacgaagg ttttacgttt
     1381 tcccgtatcc cctttcgttt tcatccagtc tttgacaatc tgcacccagg tggtgaacgg
     1441 gctgtacgct gtccagatgt gaaaggtcac actgtccggc ggctcaatct cttcaccgga
     1501 tgacgaaaac cagagaatgc catcacgggt ccagatcccg gtcttttcgc agatataacg
     1561 ggcatcagta aagtccagct cctgctggcg gatgacgcag gcattatgct cgcagagata
     1621 aaacacgctg gaggggtcat ccggcgacca tttgaggcca aacggcgtct ctttgtcgcc
     1681 aaatttaaga tactgctcct ccccgcagtg cgggcaggca acatgaaaac gcataaaatg
     1741 cggggattca ctggcagcac gctcaatctg gcaggtgcct ctcactttag gcgtcgagcc
     1801 acggatggac tttggccaga ccgagccttc aatacgcttg tcgcccagga acgtcgggga
     1861 gccttcctgt tcaatatcat catcaaaggc agcaagttca tcataacccg ccacatccac
     1921 cgacttttca cggtagtttt ttgccgcttt accgcccagg caccagaagc cacgaccatt
     1981 ggtgaaacgc ttcatggtga gcgtgttatc ccggtgcttt ttgccatacc acggagccag
     2041 cgccagcagc gatggaatat cacggatggt cggctcgacg tgggttttca tgaagttctc
     2101 ggcatcacca tccgtcggca accagataag ggtgttacgc tgtttatgct ctatgaagta
     2161 ggcataaaca cccagcagca ttttggaata accgacacgg gcagacttca ccacattcac
     2221 ctcacggatg tagtcgctgc ccatcgcatt catgatggcc cgctgaaagg gcagtgtttc
     2281 ccagcgccct tcctggtatg cggattcttt cgggaggtag taattagcat ccgcccattc
     2341 aacggcggtc tgtggctccg gcctgaacag ggcacgaagc ccggcgcgga caaaatgccg
     2401 cagcctgtca acctgactgt tcgatatatt cactcagcaa ccccggtatc agttcatcca
     2461 gcgcggctgc tttgttcatg gctttgatga tatcccgttt caggaaatca atatgccggt
     2521 tatccagctc aggaaaacgt cgctgcaccg acagaggaat accgtcgaga atactggata
     2581 tttcccttgc tatacgcgac agcacgaaag tacagaatgc ggtttccacc acttcagccg
     2641 attctttggc atttttcagt tcctgcgccg tcgcctgagc acgagtcagg cgatggcgct
     2701 caaattcaag tgttcctggg tgaagatctg cctcgctggc cagccgcagt tcttcaacct
     2761 cccggcgcag cttttcgttc tcaatttcag catccctttc ggcataccat tttatgacgg
     2821 cggcagagtc ataaagcacc tcattaccct tgccaccgcc tcgcagaacg ggcattccct
     2881 gctcttgcca gttctgaatg gtacggatac tcgcaccgaa aatatcagcc agctgctttt
     2941 tgttgacttc cattgttcat tccacggaca aaaacagaga aaggaaacga cagaggccaa
     3001 aaagcccgtt ttcagcatct gtcgtttcct ttcttttcag ggggtgtttt aaataaaaac
     3061 atgaagttac ggcgaagaag aacggaaacg ccttaaaccg gaaaattttc ataaatagcg
     3121 aaaacccgcg cgccttccgc cccgtagcct gccggatcgc cggaaaggac ccgcaaaggt
     3181 gagaataatt atcacttgca ttaatatccg gcttcttcca ccatcgcacc ggacgggcga
     3241 ctatgaggga caaaaccgcg caccataaac gcggtaaaaa cccggtgtgc atcgtttttg
     3301 attattcccg cacactcgcg cagaaggagt tccccgtcgg gctacggtca tggttaatgc
     3361 gggaatacag cgacgataca gcgcatgatg tgtcaggctt gaataccttt atccgttaaa
     3421 agggatatca gttaagttat cccgtgtagg gtataagcca ttatcaaagc cactcagtag
     3481 cgaatggctt ttgtaatgga ctatttaatt agtgatgctg cgtattcgat tagatataac
     3541 ttaggtgcca gccaaatttt taaccatgtc atattggcta ctacagaaat aataaaactg
     3601 aaccatagaa taaaaactgc tactaatggc aagacaaaac cacttaacct accgtcactt
     3661 tcccaaatca tagtcggttt gtatttaggc tgtccattat cacatgagcg tccttcatta
     3721 tcgaacttac caatttcaac ttttttgcac tgtttcttca taaaccaaat aaccagcggg
     3781 attgttgaaa tggctattaa tgttttaatc aggctatcaa ccatattcca taccagcaac
     3841 tgatgaacaa catccggaat ctgtgcctga ctgaatgcaa tggcggaatc aattccatcg
     3901 ctggccttct tcaataaatc aacaagaatt ttatttgctt cttcttgcat gtttatatcc
     3961 tgtaagttgt aagcctgttc gaaaaaaata aacaactacg agagacagaa aaaatatctc
     4021 aggctctctt tctataagct tttagttgag gatgttctgc aaaacaaaac tggttagtta
     4081 tgtaactgct tccggaatac aacagccgac gtgctacaac gtaaaattcg tttgattgtg
     4141 gcagttgagg aaacagactt gcatttctca acatcatcaa taatgaccac atccggtaca
     4201 ggaatgacgc gggtatgcaa tgtttttgca ctaataacat cacagccatt cagtggtcga
     4261 atatctgcat cttcaggcac taccagaagc acacgttttt ccggtgcaga aaagccagca
     4321 gcaagagtgc gtgacaataa agtcattccg ctgcgtggct tcatacaaat ttcagaaata
     4381 tgattctttt cgtccaaatg atgctgaaca tcatttttat catcactcaa caactctgct
     4441 gttaatacaa tccctgctgt ctcagatttc caggaaatac tgatatcctg atcatctgca
     4501 tcaacatcat atttcatcga gtacggacta aagctacgtc caacgaacgc atcatttacc
     4561 agtacacgcc caccctgtct gacctgaaag tcaacgcgcc ctgaaagctg atttgctgtt
     4621 acaagcaatg ccttttttct ttttgtttta ttgctcattg ccaccaactg ttcagccatc
     4681 tctcttgtca cactaccaga aactctttct tgaattttca cagaaaattt tctggatgta
     4741 gtagcatcga cattaaaaaa tctggatgtc atcaggcagt tattaacaat tggcatcagc
     4801 tcacttacga ttgtaaaaaa ttcccggtaa agctcatttt gcttttcaac acacagcttt
     4861 ccacaatcgt ccagcttacg ctcaagagca cttaaccttt ccattattga attaattttt
     4921 tgcaactctt tcatcaaaac ctcctgacaa aaataccgtg cgaccacggt cgcacgctcc
     4981 tgaatacatg ccctgtttct tccaccctcg cacaggactg gcgagcatga gggacaaacc
     5041 cgcgaatcat tagcgcggta aaaacccggt gtgcatcgtt tttgattatt tccgcacact
     5101 cgcgcagagg agttccccgt cgggctacgg tcatggttaa tgcgggaata cagcgacgat
     5161 gcagcgcggt atttaatgtg cgaccacggt cgcaaacgag aaacaaagag atttttatgc
     5221 tacagaggaa caagctggcg ggtaattcag cgtagtgaat accggcatct ccatcttgta
     5281 tgcgtaatga tactcagcag ttgctccagc agacgctttc cagccaggaa gcatcaaaat
     5341 agcatcagca caacgaagca ttgcaaagca aatatccatg tattcacgtt gtgtcagacc
     5401 attaggcaaa gtggctggat tcaagacaga gtgaccatgt cgtgacaaac gatctgcctc
     5461 tttattaaag gcgtcacgat taaagttctc atatcccgtc attggcccgg caatataaat
     5521 tttcattcac tatccaacaa attgagctcg tttttgatac agagggatta attcaggcat
     5581 tgcgtcttga tgtattcctg caagtagtta acctgcgcag ttatcctgtc gatcccactt
     5641 cggagacggt aataattgag ttcagcatct gctgtaagtc ttgggctttc tccattgccc
     5701 atgccgctgg ctccggtcgt tgactttgca caggtggcgg cgacttgcag gcgcttacgc
     5761 ccagcagaaa catcagcacg gaggctttcg atagtcgcgt tagcatcagc aagctccttt
     5821 gtatatctgg catcgagttc tgctacgtca cgttgacgct tctgcatatc agcgattgtg
     5881 gatgtggctt tatcgcgctg ctctttgtag gcgatggcgt tatcacggta atgattaaca
     5941 gcccacgaca ggcagatgat gatgcagata accagaccgg agataatcgc ggttaccctg
     6001 ctcattgttg accccacaaa cagacttcac gctcaatctc acgacgagtc atcaggcctt
     6061 tccattgctt accgccagca gatatccagc gacgtagctg atcacatgcg cctttgatat
     6121 cgccctggtt tattttgcga agaagcgtcg atgttctgaa attaccagcg cccacgttgt
     6181 aaacgaacga gtaaagagcg ccgcgcgttg tttccggtat atcgactttg atgtacgggt
     6241 taatttgtct ggcgacagtg gcaaggtctt tattcaggag agctttgcat tctgcttcgg
     6301 tatacgtttt accgagcatg atgtcttttc cggtgtgtcc gtaacataca gtccatacgc
     6361 caacaatatc tttgtatggt atgtaactga caccttccag accatcgtta ccacttgggc
     6421 cagtgatgag catagacgct atggcaatag ccccgccacc aatagcagct gtgacagctt
     6481 tgcgtaatga tgactgcatc atatttcctt cggtgctctc ttccccagct ctccaataac
     6541 catcgctgta gcagaaagat tattggtatt agttttatcc aatatgtcct gcaatatctt
     6601 cgtgcgcttc atctgctcgc gtttattgag ccggtatgta agcacaccga ggataatgct
     6661 gaacgcgaca ccgataatga agccccaatc ctgcaatgac aggctggcaa agaacgcagc
     6721 aagaccagcg ctaccataag atgcactgct gtatcgttca tccatcttca tatctcttac
     6781 ctcgcaatag ttgctgaggt ttcggcttgg aaactatatg gcgaaaataa cccctaccaa
     6841 aaacggcagg ggaatatatt ctccagttac tcaaataact ttttcacttc atcaactgtc
     6901 tgaagataac gttcgctctc aatctcaata cctattgcct gacgtcctaa cttcagcgca
     6961 gctttaatag ttgagcctga tcccataaag aaatcggcaa cgatatctcc ggggcgtgaa
     7021 ctactcttta taatatgttc catcatcgct gcaggttttt cacatgggtg cttgccagga
     7081 taatattgca caggaggaaa ctcccataca tcggtataag gtacatcagc agtaacatga
     7141 aatggacggc gtaaattttc atactgagcc ttcaaatcat catactgctt ctttacttcc
     7201 tcataatgca atttcagatc gctataacgc ttttcatgtg ctgaataatc aacattaaaa
     7261 ggatatggac acgggacacc gagtgaatct gccttcctgc gaaataattc gcatagttta
     7321 ttaaaatcaa ttaatgatgg aagccgccac tgcgaataag aaaaccagtg tgaacacatc
     7381 tgctttcctg tcgctgaatt aatgtctgcg gctgatatat ctaactgacg gcgagccagc
     7441 gaaaaagcat caattagtgg agaaaaaata ttttttctta aatctgcaca ttttgaagca
     7501 taacctgact gacctttagc ataacctgac gcgccgtaat gctctgcaaa taatattctt
     7561 tcagtagcag gaaaataagc ccttaaactt tctttatgac aaccgcgcca cataccactg
     7621 ggtttagccc aaatgatatg gttcagaaca ttgaaacggt cgcgagttaa taattcaatt
     7681 tttgacgcaa ggcgagagcc ggtaaacata tacaggctgc cattaggggc caatatccgc
     7741 caaaattctg ccaaaaattc gtcaatccac cccaaaaagt cagcatcgct atcccactgg
     7801 ttatcccatg cgttagcctt tacgccgaag tatggtgggt ctgttgcaat caaattgacc
     7861 gagttatcag ggatggtttt gataaattgc agagaatctg cgcaaacgag ttgtgcgcca
     7921 tgaatattag tggttttgaa catagctatt atttttttgc ctgggtaagc taacccggcg
     7981 atgcgcatcg cgggtgggct ttaggttcag cctatacctc tggcatgggt tgaccgcagg
     8041 acaagctgca acttgtcctg cgcccacttt tcaggcacaa aaaaaccgcc ataaaggcgg
     8101 cttgttggct gttgggtaca aaaaacccaa cttaacaaaa caataactaa aaaccgtcat
     8161 tttgccaagc tttttcgttt ttgtgctgtg cgaccgtggt cgcacagatt ttagaaagtt
     8221 gcctgcttat attcatcggt caaagaatat cgaccagatc cgctacgctt tgcaacccca
     8281 aaacagatca tctgttcgat gataaattca acggcagcct gactcaaaaa acaggcttcg
     8341 cttaactctt tcagggaaat gcgtgggtat ccacgcataa cacattccac actcagggcc
     8401 gcttcagtca tatttccacg aatttcatta atattcataa cgcctctcta atcaatcttg
     8461 aaactggaaa tcaacatcag ccataaagcg gttcaactca gccagttttg gccccatagt
     8521 cccaactaag cgaccagcca gtcggtctgc taggttggta ctgttgaaac catattcctt
     8581 ttcgaaacgt tcaaccttct gccacaactc atacagttcg ttagctatct ccgcaacgtc
     8641 gtcgcgcatt ttttcgttac cttgataatt cataatatcc tccaaattca tatagttagt
     8701 gggttattgc ccctcaatga cacgaactgt aactctgcca aacgaacaca tccagtgtta
     8761 ttttcacttt ttagtgaaat ttttaatttg cgctgttttc ttgttttggt gtattttatg
     8821 gatatggagg ctataatatg tttaacgtga taacccaccc tgcagcactg gatgaactgc
     8881 ttgaactacc agatgatttg cgaggtcgca tgaccagatt aattgagcga ctggagaaag
     8941 aaggcaacaa actgaagatg ccccatagcc gtgtaatagg tggagggctt tttgaactac
     9001 gagtagggga taaaaacatc gcaagaacgc tgtacgccta cgcgactggt aacgaaattt
     9061 atctattgca tgcatttgta aaaaagacac agaaaacacc ggtgaaggct atcgaaatcg
     9121 ccagaactcg cctgaaggag atgaactgat gaaagcgaaa ggcatcccgt ttaacgaagt
     9181 aaaagaaaaa ctcctcaaca ctccagaagc cgtcaaagga tatgaagagg ccgacaaaga
     9241 gctggagatg gtcgaaatgt tatacgaaat gagggaaaga gcaggtttaa cgaaatcagc
     9301 tctggctgaa cgaatggggt tgcgaccgtc agccatcagc cgactggaaa gcaatccttt
     9361 aggggcgagt atgaaaactt tggctaaata tgctcatgcc tgcggcgcaa ccatcgatat
     9421 tcgcgctgtt tattaattag actcttttac taccccgtcc agaagggcgg ggtgtgcatc
     9481 aatgatgtga acgctcaaca gcgatagttt tctgacaatg ctgggcaatc tctcgcgaca
     9541 tgttgatgag tcgtgaaacc tcaccaatat gtaggctggc ttctggagaa gccagggtga
     9601 cgctaaccag atccagaacg gcgtcaatat ctcttaaatt tgaatcgagt tgttcaagag
     9661 ttgatacggc tgcttcagac attaaatctc ataccattta actaaaaaag gttatttata
     9721 ctgtataaaa ttacaggata aaactcaaac tttacggtca attttcaatg aattaactgt
     9781 gtaaactgct gtgtggaccg atacttatgc tctctcccta tgccacgatc aactttctca
     9841 agaagtccag tacggacagc agaatcaagc atccttttaa caattctgct atttaaccca
     9901 acatcaaaag caagcatcga agcgaaaaca aacccatcac caccatcaga aaagctcttt
     9961 tcgctccgtt ttcgtatcat ttcaaaaagc aatgcctttt tatccattta ttcctccgtg
    10021 accagtcacg ctttgagcgc aagtttcagc ctcgtagttg taaggtggca tgacccttcg
    10081 ccatcaaaaa tgcattctga aacaggcatt ttttgaccac atcgcgggca agtagtagat
    10141 agctgcttct gcagctcctt atagtttttc ctgatcagca aaccaatgac ttcgttctcc
    10201 gaatatggaa ctcgccctgg tctacgcaat acacaaattt cccccaacat acgtaactcg
    10261 tcaggttcta gaatccagtc ccgtcttgat gtgccttcct tcttcaaccg ttccctgcgt
    10321 ctcctttgcc gttctgccgg tgtcagcgcc ataaatcctc ctgaaaatta cttaacccaa
    10381 ttaactacac cgccagcggc cacaaccgcc ctttcacagt cacgctcata tctcaaaggt
    10441 tgaaatacac catcaataaa atatttatct ccaccaaacg ctgcaggaag tgaaacagaa
    10501 aactgaatat gaccatccgg agttaccgga gagttgccag ccagtctacg caacacagcc
    10561 ttaacggcct caatacggtc atcatcaact ttttctacaa tattggaatt taaatacttc
    10621 gaagccttgc tatgccaatc gctggtttta gggtcttgtc catccatcaa acgccaagct
    10681 tcagagatta actcatagcc agtgacagtg gcttcctgcg catcaccaat aagtcgtgta
    10741 atctctcttt caagccgacc gcctagctct gttttagtgc aatgttctga ccaatcgcca
    10801 gcctcaagca gcgcgagaat gcttaaaata tcgtccaata gaacggtagt attatcgact
    10861 tccttctgaa actcaccggc tactgattta tgcatcattc gtcatcctca tccgagtcat
    10921 cctcatcaca ggatgagagt agtgggttag tcattcgccc tacttgagtg gcgtagccac
    10981 gccgacagag attgcgcagc acactgtata tttcgaacat ctcggttcgt tcatcgccaa
    11041 catcaagctc agatgctata gcgtggcatt cagtcgcgag agccgatatt ttctgaaata
    11101 actctgcttt attcactctt agactcctgc ggaggttctg gtagcggcat ccagtgagtt
    11161 gcttgctcaa taccattacc cggcttaatc gttgcatctc cgcgccgaaa ggtgcttccg
    11221 gtatagcgtg cggagcatat tagcggttca accagagagc tatcgaaatt caccgaaata
    11281 agcacgttct ggcccttttc aggcattcgc tcactacagc ttatccaacc atccggagtt
    11341 accggatagt tgccagcctc atacgcgacc cttatccagt gcataagcat ttcagtactt
    11401 acacatccac aatcaacatc aattttgtca tgctgctgtt ccagccattg ctcaaaagtt
    11461 aacttgtaag tttggcttac aggtttggct tccagttctg ctatgtgatt tttggctgat
    11521 tccaactcaa tcgacaattt ttccaactgc tctttatgat ttttatattc ctgatatgcg
    11581 tgccaagact gacctttgcg cacactatca gtgatatcag taatctgttc tggtgttagc
    11641 gtggtcagtg gctgtgatgg gaaaataagc actttcccgg aatcccaatc aaaaccagcg
    11701 tgaattgact gaacctcaac tgaaggtgtt gaaccaatgc tgccaggcga atgaacaacg
    11761 attgttacat ccatatcgcg acgatggctg tggttgttgg acaaaatacg attcaccaac
    11821 tcagaaaatt tggaaaattt catgctgatt cccctttctc tgctctctcc tgtcggaaca
    11881 tcactatcat caggtcgcct tttgtcgcta tcctggctgt tgtacctggt tcaatgcggc
    11941 taagctcaaa tgcgtcatag aacgcttcta atgccttctg gcgtagttcc tgtttgcgcc
    12001 gttttttcca ctgttttagg aaaatggaac ccagccatcg ccatgtacgg gacatgatgt
    12061 aaagccaacc gagaagtgcc agaccggtat ttagggccgt atcgatcgtt atcgtggtgt
    12121 cgatattcac tacgccacct cctgaaaatt tccctgataa aaagccagca cacgctgcat
    12181 aacttcactt ttccggcaat cgtgacaaat catgttcaag cgcctgtcgt aacgacgtat
    12241 ttctccgtca ggtaatgacc agataaggtc tggatcaacc actaccgatg tcttcggctt
    12301 tactcttgat agttttttac gggcgttttg ccagtccttg cgtgcttgtg cagacagaaa
    12361 tatcccggag ccagagatat acaaatcacc actggcagca agctctcttg ataaacgact
    12421 aacagcacat cggctaatac cagtttcatc cgcaagttgc ctaaccgttc ctcgtccatt
    12481 caggcgcacg agttccacaa tttgtttatt cagttcttcc ctctgttctg gtgtaaaagc
    12541 ctttgccatt acgattctcc ttgccctttt acaagacggg agaaaattgc ggatacatat
    12601 ttagcctgat gaatagcatc agccagggca ttgtgtcgtt cgccatcaaa tggcatatct
    12661 tttttaggat taaaaccaat aaactgacca agattaacga ttgttcgaac atcctgatca
    12721 ttaaaatatt cccacggaca ggctaagcca acgcgctcat aagcaccgcg taaaattaca
    12781 ttatcgaacg ttgctccatt tccccatact ttcaattttt ttaaattaca tgcatggcaa
    12841 gtaataaagt gtgataattc aaaaagtgca tccttaatat caagggcatc attagtacaa
    12901 attgcggctc tggcttcctt gctctgtttc aaccaccata aaatggtatc accatcggcg
    12961 acaccaccat tcaacatcga gcttctgaga ttcactggca cataaaaagt aggacctaat
    13021 tcctcacttt ctggagaaaa aaacactgcc ccgatagaaa caattggagc acatgtgtta
    13081 gtacccattg tttcaaggtc aatcattaca tcagaccgtt tcctgtcgta aataatctga
    13141 ttagtcacga ataattctcc tcaacgttta tctataccca aattttctgt tgatatgttc
    13201 tggatggttt tggcgttctt tctaattcag gtaaatgaac ataaacgcaa tatccatcac
    13261 ctaaaaaatc agaacgaaca accagaactt tcttaccacg ccgccgataa gtatctgaaa
    13321 gacgctctgc ttcatcatgc gtcatcggcc cctgcttaaa tggcgttatt ttcattttct
    13381 tgtgcgacca cggtcgcacc ctctctgatt tccaagtaac gcttaatcca aagattttct
    13441 atatgcttat tacctggctg atttgataaa taccaatcgg caattacaga ctgcttatta
    13501 ctatctggtc gtgtcctgta accacaagtt ggacaccaga taacatattc atttcttacc
    13561 cctgaatacc ttaattctgg cttcgacggt ttcctgaaca taacttgttt acaaaggcag
    13621 aatggcactt cctgcccgat ggcgtttgat gatttcactg cgcttctccg ccgcatttaa
    13681 taatgcagtt cgatttaagc tcagacatcg cgacttaatt aaaagccagc gttctttata
    13741 atcttgtctc cagctatcaa tagatatatt cagtagaaaa ctcagatgcc tgtcctgttc
    13801 tctctcatca actgcctcag cacacgtaag ttctgattta acctgctgtg ccgcgtaata
    13861 aatcaatttc cgcatggctt ttttcgtgga ggccttcatt tttttaaagc cgccccccga
    13921 atgagcaacc agaaaatcaa gccataacca ctgacaaaca atcacatcat tgttgtaatc
    13981 aggcttgcat ccgtagcaat aataaagcca ggctgattcc tcgttattca actgaccaat
    14041 agccctgcgc cagctggcgc tctgaaaatc aatatcagtg agcaacattg atgattgttt
    14101 aaatgttttc ccctggtgga atctaaccgg ctcagcagga acggacactt cgtatgagtt
    14161 atcatcccct acactgattg accggactgg tttattgtta aaccgtcctg atctaccgag
    14221 acgcatctgc tcaagctgga cctccagaat gccacgctgg cgataatgaa catcagccaa
    14281 tgccgtagag acgcagctgc gtatatactc aagctccacg cttacgactc tccttaatcg
    14341 actgctttac ccgccgagtt gtaaagtcac tcttcagctt ataagcagta cgaacttcaa
    14401 tatcgctttg ccgcaatgaa ggaatctctc cagcggttaa ccattggtaa acagctccgg
    14461 gtgttacacc tacccctgcc gctgcttttt ctacactgcc aaaatggcga ataagttctt
    14521 ctggtttcat gttgttatta taaacaataa acaacaatta aagccagcta taataaataa
    14581 attttatagc cagctataag aagatcattt atgattacag tatgaaaaca cgaggcgaac
    14641 gactgaaagc acgccgttta gagatgaagc tgacgttgcg agaagttgct gaggctgtcg
    14701 atatctctat agcaggcgtc caaaacttag aacgtggcga cgtaatgccg tcactggaga
    14761 taggtattgc cctggctaag tgcttacgca agccagtgca ttgggtgcta tatgggacag
    14821 aatatgaccc tgatcgcatt cctgttattg gcacaactga aagtgggccg gatgaagagt
    14881 ggaagccggg acaggttcca aatactgagc gattcctgcc attcatcagc cagcgcaata
    14941 ctgtgtatgc gttaactatc agcaatctta cgcagggaaa ctatcagcca ggcgattttc
    15001 tcctggtcga ttctgcgctg gaactggtcc ccggcgagga tgttctggtt cgtgatactg
    15061 caggaaatat cactatccag cgcctggccc gttttgatga tgaaaattac tatctggatg
    15121 gaacaaactc acaacgcgcc atcttcagga aaagcgatct tgaatttgtt caccaaatag
    15181 tcggaacgat caaatcattc atggttgagg gtagatgact taatacgggg tttattgttt
    15241 gctgtaaatc tggtttaatg cagggcatat gttctgtggg ttgactcagg ttacagcagc
    15301 agaaaaaaga cgaaaaaaaa cccgagtcgg caaactcggg tccttttcag gaagcagcca
    15361 cagtaacgcg acggattccg tcaattcaag atgcgtttat tgtggctgct cctgcggatt
    15421 ttttcaaccc gaaaaaacat aattcgcatg gaatggctaa aaaatgacat taaacgattt
    15481 ctatgcggat cgctttggca gcgatccgtt ctcgctaatt gaagcagcac gggatgaact
    15541 aaccgagctg gcacagatgg ctggtatcaa ctggcctgcc tgttccgaca acatccaact
    15601 caaccctcgc gggggggttg agcgttactc ttcatacaat aaaacatctc cagaagctct
    15661 cgaaaagagc ctgaaaggac gcgtagaaat ttactccagg ctggaacaca gcaaagggat
    15721 tgattacccg ttcattaact tcgtccataa aggcagcgat gctggctcat ggagcggttt
    15781 ctccttcctg ttttctgaat atcgccgtga gcaacaacgg aataatgcga ccgtggtcgc
    15841 acaaccggaa gaagaacggg aaagaataga acgtcaggcc gaagcacgtc gtcgccgtga
    15901 agaacaaaaa cgaattaatg acctgaaaaa caaccaaatg gaccatgaac gtttgctggg
    15961 atggctggca ttccacagcg cgtgggaaca tgctccggct gaagacggct catggccata
    16021 tgcgattaaa aaaggtattc gtgacgtttt tagttcatgt gatgtgcgtc gcgtgaccag
    16081 tcacgacagt gcgaaatgga gcagagggcc aaccacatat atggctatcc ctctggctca
    16141 cctggacggt cgttacgatg gtcgtattgt gggttggcag cgaatcgaca aacaaggtgg
    16201 gaaataccag accagcgcag taaccagcgg cgatttcgtc ggtgcctgct ttgttatcgg
    16261 tgagctgaaa ggggcgcaaa acatcgctgt tacagaaggt tttgctactg gtgcatccat
    16321 ctggcttgct acgaaaaaag ataaacgctt tgacgctgtg gttgtcgctg tatccgctaa
    16381 caacatggtt catgttgtgg aacaactggt aaacatgtac ccggcggcaa aaatcacctg
    16441 cgcactggat aatgaccgga agtcagccac agaaggaaaa gggaataccg gcctgcggac
    16501 tggctatgaa atcttatcga aattcaacga tgtccgctgc gtctatccaa cttttgaaga
    16561 cgatccgcaa atggagtgca gtgacttcaa cgacctgcac aaactacgtg gacttcgtga
    16621 aacagcacgt caactatttg caacagcaaa ccgcctgaaa accggtactg atttgctatc
    16681 tctgacgctc aaccgcctga agacaattaa gcgggataac cgtcgtactt ttgccaaaga
    16741 gctgctgaga gcggtggata ttggcatgct aacctgtccg gttcctaaca gtccgtcaga
    16801 tttatttaac atgttctgca tcgttctgcg tgaaatggaa ctggaaaata tctatcgcgc
    16861 tacggtaaaa gaccacattg cccgtcgcct gaataaaaag tgccgtattg cccaggctcc
    16921 gcgctctttc agcgaacgca taactgatcc taataaacgc cctcaacata taacctataa
    16981 gcgttttgaa acttcggtaa tgaccgatga tgtcctgaag tacgtgcaat cactacaggg
    17041 gatagtcatc gtccgtgctg gtatgggttc cggcaaatct actggattgt tacgaccgtt
    17101 aatgcatagc tcagaacgtg gtatctctgt tgctcaccgt gtatccctta ttggtggcct
    17161 gtgggaaatg atgacagaag gaaaaggtgt aaaagccgat atcctgcact atcaggaccc
    17221 tggctatcag gaaatggctc catacgccag taaactgacc atctgcataa actcgattgt
    17281 taaaaactgc tggcaaccgt tgatgcgcca gcacgactat tttggctttg acgaggccac
    17341 acaagggcta cgtgccgttc tttccggtcg ggctatggaa aacccggtgg ctgtattcaa
    17401 tacgctaatt gacgcgctgg ccagaacgga acaacaccca atcatggtgg atgctgatgc
    17461 gaacgatctg ctggttgacc tggctgaact ggctatgaaa cgccgtgaag aactaggttt
    17521 accggcatgg ctgcagatcc acgtaattga attacctgtt gatgttcgca accgcgaaac
    17581 aggtgagcca atccgggttt tttatacaga aaaagatcgc atcatgaccg aggtagtgaa
    17641 agctgtatcg gatggcgaaa aaatcatgtt ggccaccgac agctccacct ttgcagaaga
    17701 tgtgacagcc accctgagaa tgcattttcc aggtaagaaa tttctgtgtg taaaccagaa
    17761 aaacaaacca gagccagaag ttgaagcatt taccaatcaa ccgaaaaaga tggtcaaaaa
    17821 atacgacggc ctgatttata gcccgtctat ttcatccggc gtgtcgattg agcaaaaaca
    17881 ttttgaccgt catttcggca tgttctgtgg tgaagttgta cccagcgacg cagtgcaaat
    17941 gctacgccgc gaccgtacag caaaagaatt tattatcggc tttgataaag ctcgtgcaaa
    18001 acgtgaaact gacccgcaaa aaatagaacg agcattcgcc caggcattgc ttgctactgc
    18061 aggtatgaac ggtgaactga cggatgtcgt tttcgatggc gaccgtatat caatgggggt
    18121 ggccaatacc gactttacca ggatgaaaat taaagcagcc gcaattgaag cgtctgcacg
    18181 taacgactac gccagcaaca tgatctgcat catgcacagc gacggttata aggtatcgcc
    18241 gctggccacc gattatgctg caaatgatat cggtaaagat ctgcgtaaag aagcgcggga
    18301 tattgtgtgg gaacagacac tggatctgca tctcaataca gaaactccac aagaatcaga
    18361 gcgtgaagaa ctgctaaaaa aacgcgcgct gacgcttgaa gaacaggcaa aacttgtccg
    18421 ctgggatatt gaaaacgaac tgaaactacc ggttgaagaa ggcacgctaa aattctattt
    18481 cgatggtgct cgagacaaag ttcgccgcta tgaaacaatg cttttagatg aggtaacagc
    18541 tcgtcgttac gaccgggaag aagcagccat caattttacc tacgcatacc gtcaggcggg
    18601 acagtggcaa tattttgtag tgaccgcgat gactcgcgag caggctgatg atgtctttaa
    18661 aactaaattc cctggaataa tcgatcacaa ggtaaaagct actcctactg ttgaagtcgg
    18721 ggcgcgcggt ttctatgcgc ttaaatcagc aactctccgg cagtacttta tcgactgcgg
    18781 tatcaaccct gagactatga ccggcgaggc gactcaggag cgtctcaaat tcgctcgaga
    18841 taacctcatg acagccgaac gccgtgacct gttgaataac gttctgcaca ttggtggttt
    18901 tatgacgcca aaagggaagc ctaaagtgcc ggaagcgttg tttaaaaaca tttgcgactc
    18961 tcttggcctt aaaacaggta aacgtcgcgg cagagacggc gataagcgtc caaccttgcg
    19021 ttttgttgag cctgaatcag cggcatttat gctggatatt cttgttcagc gtcagaaaga
    19081 tggcctaacc ctaaaaacgc gtaaagccga gaagacagcc atcgaaatgg atcgcgattt
    19141 ggatctcaat atatatatga atgataaaac gcgatccaca aacgagcagg ttcaaaacgc
    19201 ccctcattca gtaatttctg aggctttggc cgtgttgcca gtggctgtac ctgagtcctg
    19261 ggcgatgagt gcgctttcca gtgaggagtt ggccactatg tcatcatggt catcagccag
    19321 catagctatg acgtttgcat ccctgtacct cacagagttc atggatcacc tttccagcaa
    19381 tgagctgcgg cggttacgtg aatacattac ggggaatagc tcatgcagct acgaagagca
    19441 gggcgctttc tatgggtaat gattggatat tatctttgtg tctcttgctt tgtgtgttcc
    19501 atctatggtt ttggttcata gaccgaaagt ctcacaagga aaacatgaag gccaggaagg
    19561 cgatgtttcg tggtgaacaa tttgcgcaga cttatcgtat gtggaaacgt ttctatcgtg
    19621 aattttcggt gaacggggag taaaaatatg tctactactg catttttgga caaacttgat
    19681 taccgtcagc tgcagttctg ccgggatgaa tgcgaacgac gcattagggc tatccaggag
    19741 gaagaaaaaa aggtcgcctg ggctgtaaca gatcgtggcc ttatctttgg ctggttccgt
    19801 acggaggatt atctgaaagc agtggaatta ttggcgcgta aggctgctga gcgttgggaa
    19861 cgagccgaca aggaaaaccc ggagacgaag tatgaactga atctctacat cgagggcaac
    19921 cgtctaccat tgtcagagta taacgctttg tttgctgatg gtgagtgggg ttaaaaatgg
    19981 ctgttcaatc atacaaatac gaatcccctt tggataaggg atacattcgt atcaagttaa
    20041 gtcgttctca gcataaggct atttttaagg taaggaaaat tcgcatattg gatgcgtatg
    20101 cgtattacta caacggtgaa aatgttgttg ttgaacactt tcttgcacga tggtttgtag
    20161 ctctgatttt tgtccctctc ctgttaattg gtacttttgt ggatggtttt cctagtacat
    20221 ggcgggagat aaagaaaggc ctattcccgc ataagtacgg cagattttcg caagatagct
    20281 ggcgagttat acctggtaaa cattctgtag atgagcaacc gattgttgat tcctggttga
    20341 atcgtatggc tgatgctaaa aaagttgatt gaattgtgat gactgttaca ggagataaag
    20401 tgaagagttt tatttttgtt ttatgttttg ccttttctgc tgcggtttat gcccaggaag
    20461 atagcatttc tgttattaac tcatcagcgg agcttgcatg taaaaataat cccgatgctg
    20521 catcaaaaga gtcatgcaaa gagctgctcc tttcgacggc tgcttttgct tctgaaaatg
    20581 caaaatttta ttcagccttt tgtcgcggct atgttaagga cgaagataaa gaaaaatgtg
    20641 atgatgccgg taagttagtt aggtatttcc aatctcttaa tcagtaatgg cctttgatta
    20701 acgatcaact tctgatcact cgttcaagca aactataaaa acacctgtcc ccgtgactgg
    20761 tcacggggta aaatgtcttt gcaaattatt gacgtgcgcg tcgttttgac ggtaaagttt
    20821 actccgctac ggtaaatccc gtagccgggc gtaggaaccc gagtaaatga agcgcacaac
    20881 acgcgccagc gtgttttttt gtgtgtaaaa tctgcgtaca ccaaatttat ggtggctcag
    20941 atggggcagc ttttagctgg ccggtttctt cgtttaccgg tattcctacc cccgtctggg
    21001 ctaccgccat atagagcgta ggaactctgg tggtagcacg accaattaaa cggagtgcat
    21061 accatgttca aattcaaatt tgcggcaatc gttcgcacag acaaaaagtc acacatccat
    21121 catctttcca ccatcgcgtc atctgaacgg gaagctcgtc gccagttcgc cagccggttt
    21181 gtcctggtat tgtctgcccg tattccggtg cgcgaggtgg ctgcatgaat caggtaaaaa
    21241 tgaacactgt tggcctgctg gaatcgttgg atgaacgtct ggctcaggtc tatgcgctcg
    21301 tatcatcagc ccatcggaca atctccagtt gtgaggcgtc gatttatctc tcagaagcag
    21361 ggaaactcct tcaaattgcg tgtgagctaa cacacgaagc acagggttat tcagcatcgc
    21421 tctcgtcgca attgctgcac gataaggagg ctgaatgagc gcgttagcag tattttcctt
    21481 tcaggaagag catcaggttc gtgtagtgat gatcaacggc gaaccgtggt ttgttgcatc
    21541 tgatgtgtgt atggcggcgg gtattgattc tacggccatc agaaagttgg atgaggacga
    21601 aaaggggcag gcgaggtaga ccacacaaca aggcaccgat aattcggtgc ctttaccgta
    21661 tgctttatcc gaaatatgtc agccatgcta actgcattgc tgatataggg ctgtttgcac
    21721 tgccggacca agcataattc tggccattat aatgaaactc aacaacgtat gagccatcac
    21781 caatatttct cacaggctta aataccggct tagatacagg tgaagaaggc gcgggcggtt
    21841 gctgttcttc tacgctctct tcctcttcct cttcctctat ctcaatttcg tcgtcatcca
    21901 gatcgtcatc ctctgataat tcatccgtct gttcaggtat tgcttccagc tcttcttcgt
    21961 caggcataac gatttctgga gaatctattt tcagctgcca ttgtccattt tcgccaacaa
    22021 attgccccag agcatcagca gcaaattcca ggtatcgact aatcattgta gggctaaatt
    22081 tgaatgctct tagagtgctg ttcgtgattt ttatagaggg gtcctgctct accagctgct
    22141 taactgtgtt atgcagacga actccggcat ctcctctggc gaatcctggc atttcgtcat
    22201 caagctgctg caatgcttcc agcctggtat tttcgtttcc agtatttggc ctccatgtct
    22261 ttgagaagtt ggccagcttg aattgcttat agtgtaattg agtgttttca tcgtcatggc
    22321 ctaaaatttc cataaagaaa acatcctcat ctacgttttt ccaacgagga tctacgcgga
    22381 aaaacatttc atacgcaata cgggcataaa tagcacgact atctttataa acacggcggt
    22441 catctttgaa aaatgattta acccacgggt taaaagcctt tgctaatatg gcattaatcc
    22501 gtccgttttc agagcgtgtg tcgttatctc cataaccttt aacgacatca tcaaaatcag
    22561 atgctgctgg gcatgagcga agtacattta ttaaataaac aaatgttttt gcttcgcata
    22621 atgtatatat ttttctggtt atatcatcag aagttctttt ttttgcttgg cctaaaaaat
    22681 caacggtata cttaccagaa acagtaaatg taccttgata cataatctca atcattcgtc
    22741 ttcctgacaa tgcagcaagt gcaaatgcca aaggagccat acctgaacga gtgtttagat
    22801 tatttaatgt agcagggcta tttataatat cgtaaatagc ttgcatatat ttagggtaat
    22861 caatgaccac cacgttacgt ttcttattgt gcagaacgtc tgcccatcgt tgctggatag
    22921 atgcgcgttc ggcagggctt agttgaagat gatagagaat ctcatggtta acttttagct
    22981 gattcaactc ctcaagtaag gcagatcctt gctgtagtaa tttatataaa taatcgcgct
    23041 tatctttcca gttatcgcta gttaaatcag aaatggcgaa ttgccactct ttatatttat
    23101 ttgataattt taataactta ttatgacttt tgttatcacc aattctgata ttagataaat
    23161 cttctgataa agggagaatt aattttaatt ttgcctgtaa agctgacatt ctctggcgaa
    23221 tatcggcagc aggtagagaa agccatgata atagctcctc attatataaa ggatactttt
    23281 cagataggcg atgtatattt ttttcaaagc tatgatgaag tttatcatca aaccgttttc
    23341 tggccctgct catataggca ttgaatgtgt tggctgtaat tcttttttct aatcctttcc
    23401 ccctaaactt tcgcttgtca ttaaataatg catttttaaa ttttaatgca acagctttta
    23461 ttttttttgt tttttcccct tgaggacggt cagagttatc tattgactca acatcgctaa
    23521 caagtgagtt tattaattca ccgattttta ctttgctcat ctttcctctc ctgattatcc
    23581 gctcggatta tcgtaacaca atttgagaat aagataacta ataacgcgtt aacttagtca
    23641 caaactaaca gaacacaata acggataata aataaaaaca caatatcgga acgttaaaat
    23701 ttgttccttg actttgttc
//
LOCUS       DAZPEP010000049        23714 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZPEP010000049 DAZPEP010000000
VERSION     DAZPEP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23714
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.049"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..163)
                     /locus_tag="ACHUT9_001953"
     CDS             complement(<1..163)
                     /locus_tag="ACHUT9_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1348434.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFA"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUT9_001954"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="ACHUT9_001954"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1348435.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUT9_001955"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="ACHUT9_001955"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1348436.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUT9_001956"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="ACHUT9_001956"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1348437.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUT9_001957"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="ACHUT9_001957"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1348438.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUT9_001958"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="ACHUT9_001958"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1348439.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="ACHUT9_001959"
     CDS             5058..6191
                     /locus_tag="ACHUT9_001959"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1348440.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="ACHUT9_001960"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="ACHUT9_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFR1348441.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUT9_001961"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="ACHUT9_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFR1348442.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUT9_001962"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="ACHUT9_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFR1348443.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUT9_001963"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="ACHUT9_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFR1348444.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUT9_001964"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="ACHUT9_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFR1348445.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUT9_001965"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="ACHUT9_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFR1348446.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..10631
                     /locus_tag="ACHUT9_001966"
     CDS             9762..10631
                     /locus_tag="ACHUT9_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFR1348447.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLNAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(10834..11187)
                     /locus_tag="ACHUT9_001967"
     CDS             complement(10834..11187)
                     /locus_tag="ACHUT9_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFR1348448.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHUT9_001968"
     CDS             complement(11325..12971)
                     /gene="groL"
                     /locus_tag="ACHUT9_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFR1348449.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13015..13308)
                     /locus_tag="ACHUT9_001969"
     CDS             complement(13015..13308)
                     /locus_tag="ACHUT9_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFR1348450.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHUT9_001970"
     CDS             13584..14840
                     /gene="yjeH"
                     /locus_tag="ACHUT9_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFR1348451.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(14862..15332)
                     /locus_tag="ACHUT9_001971"
     CDS             complement(14862..15332)
                     /locus_tag="ACHUT9_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFR1348452.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            15669..17105
                     /gene="aspA"
                     /locus_tag="ACHUT9_001972"
     CDS             15669..17105
                     /gene="aspA"
                     /locus_tag="ACHUT9_001972"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFR1348453.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHUT9_001973"
     CDS             17223..18524
                     /gene="dcuA"
                     /locus_tag="ACHUT9_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFR1348454.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            18640..18978
                     /gene="cutA"
                     /locus_tag="ACHUT9_001974"
     CDS             18640..18978
                     /gene="cutA"
                     /locus_tag="ACHUT9_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFR1348455.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHUT9_001975"
     CDS             18954..20651
                     /gene="dsbD"
                     /locus_tag="ACHUT9_001975"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFR1348456.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            20688..21263
                     /locus_tag="ACHUT9_001976"
     CDS             20688..21263
                     /locus_tag="ACHUT9_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1348457.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21370..21445
                     /locus_tag="ACHUT9_001977"
     tRNA            21370..21445
                     /locus_tag="ACHUT9_001977"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21403..21405,aa:Phe,seq:gaa)
     gene            21688..21802
                     /locus_tag="ACHUT9_001978"
                     /pseudo
     CDS             21688..21802
                     /locus_tag="ACHUT9_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            22062..22361
                     /locus_tag="ACHUT9_001979"
     CDS             22062..22361
                     /locus_tag="ACHUT9_001979"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HFR1348458.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYK"
     gene            complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHUT9_001980"
     CDS             complement(22352..22477)
                     /gene="laoB"
                     /locus_tag="ACHUT9_001980"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFR1348459.1"
                     /translation="MVRLWEGEFVSGTASGIGGGEDSIISSPSSSLYQITGTIHL"
     gene            22544..23599
                     /gene="cadC"
                     /locus_tag="ACHUT9_001981"
     CDS             22544..23599
                     /gene="cadC"
                     /locus_tag="ACHUT9_001981"
                     /inference="COORDINATES: protein motif:HMM:NF007540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFR1348460.1"
                     /translation="MVFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVN
                     KSCNSWSSPYQLSYAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVN
                     QRQYRAQQCFMSVKLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQR
                     MQETLQQILPHRGALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEK
                     ALVDIVRHSQHPLDEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKIDESY
                     QAINTGIDLEMSWLNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQ
                     TSVPYVVPYLDKFLASE"
BASE COUNT         5948 a         6005 c         5827 g         5934 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc
     9901 tgattgcgct ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc
     9961 agtatgagca agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc
    10021 tggttgatga gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg
    10081 ataatcaagg cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt
    10141 tagtcgtcaa tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata
    10201 atgcggaaat gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat
    10261 acgacgttga ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat
    10321 ggaaacataa cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg
    10381 gcaaagaaca atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa
    10441 tagaggcgat cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg
    10501 tgccatgtca ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg
    10561 atcaatgctt acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac
    10621 aggcgaaata atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg
    10681 ataacgtgat cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc
    10741 tcaagcattc aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca
    10801 ataatacgtt tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc
    10861 gataatctgg ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat
    10921 gccataaatc acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc
    10981 gcgcatagaa ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt
    11041 cgcagtacca atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg
    11101 accggcagca ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac
    11161 aatccctgct aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac
    11221 tcgcgtcgtc cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac
    11281 atttctgccc gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc
    11341 atgccaccca tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc
    11401 aggtcggtaa ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc
    11461 agagcagaac gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat
    11521 tcttcggttg ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca
    11581 acagacggtt cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc
    11641 agtgcaactt tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta
    11701 gacgctacgc ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca
    11761 cgggtcgcgt gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta
    11821 gcagcaccta ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt
    11881 ttttcacggt cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg
    11941 ccctggattg cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc
    12001 acaacacgtt tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc
    12061 tcttcagaga tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga
    12121 cgatcgccga agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta
    12181 acaaccagag ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg
    12241 cctgctttgg caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg
    12301 tcagccagca ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg
    12361 aagtaaggag acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc
    12421 tgcagaccgg taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct
    12481 tcagcgatca gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca
    12541 atcgctttag agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta
    12601 acggctttgt cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc
    12661 agaccctcag tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca
    12721 gcgtcgttcg ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg
    12781 tcttccagtt cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg
    12841 aaagatttat ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct
    12901 gccagtacgt ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct
    12961 ttagctgcca ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc
    13021 ttcaacaatt gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt
    13081 ctcagatttc acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag
    13141 cggcttcact tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg
    13201 ggtggattta gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac
    13261 ttctttacgc ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc
    13321 tttgagaaag tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt
    13381 gaccagagaa atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg
    13441 atcagcacaa aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat
    13501 cgccgtcata agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt
    13561 tgggcgcaat cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg
    13621 gcattggcct gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag
    13681 ctgcacaggt agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt
    13741 tcccgattgc gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg
    13801 cgcacttcgt cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt
    13861 tatcggtcat tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg
    13921 caatgtttgg ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg
    13981 tgtggtatat cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg
    14041 gacttatcgt cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata
    14101 tccccttccc ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga
    14161 tgttctggtg ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc
    14221 cagagcgtga ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact
    14281 ggggctgtac ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag
    14341 catcgcttcc aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg
    14401 taattggcta tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc
    14461 tggtctggtc gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc
    14521 atatcccgaa taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga
    14581 ttcatgcttt agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta
    14641 tgatttatct gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac
    14701 tggcggtggt tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc
    14761 tctatgcgct gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg
    14821 aaaatggcat aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga
    14881 tcaaggcggt cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca
    14941 ccggtcccgg cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac
    15001 ttcactgtca gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg
    15061 aaaaagcccg gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc
    15121 tccgccgcag ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag
    15181 ccctggttac gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc
    15241 agcaataccc ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa
    15301 aggaaaatgg caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta
    15361 aacccggcta cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa
    15421 atttcaagat gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac
    15481 aaattaataa ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc
    15541 ctcaagcagc atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa
    15601 acattcgaaa ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga
    15661 aggttcacat gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc
    15721 cagctgatgc ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca
    15781 acaacaaaat cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg
    15841 cagctatggc aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg
    15901 ccgcatgtga tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct
    15961 accagggcgg cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg
    16021 gtctggaact gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg
    16081 ttaacaaatg tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact
    16141 cttccctgat taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag
    16201 ctgtcgaatt ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga
    16261 tgaccctcgg tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca
    16321 tccaacgtac cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg
    16381 gtctgaacac gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg
    16441 gcttcccatg cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg
    16501 ttatggttca cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc
    16561 tgcgcttgct ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc
    16621 aggcgggctc ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc
    16681 aggtatgctt caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc
    16741 agctgcagtt gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca
    16801 ttctgaccaa cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca
    16861 aagaagtgtg cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt
    16921 tcatcggtca ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg
    16981 tacgtgaagt cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct
    17041 ccgtacagaa tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac
    17101 agtaatcgta cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt
    17161 gtgagcagta acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta
    17221 atatgctagt tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg
    17281 ggggaatagg tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg
    17341 ttaaacccgg taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta
    17401 tttctgccat gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc
    17461 tgcgccgtaa cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa
    17521 tctttgctgg tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga
    17581 aggaacaagg cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg
    17641 cgatcaccgc atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc
    17701 atggcatcag ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc
    17761 tggtgatgtc cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt
    17821 atcgcaagcg tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca
    17881 aatccggtgc aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct
    17941 atgcaatcat caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca
    18001 acgcaatcct gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg
    18061 ataccgacaa catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta
    18121 tcctgggtgt tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag
    18181 ataccgctgg tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg
    18241 cttctgctct gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg
    18301 cactgaacgt ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca
    18361 ttctgccgac ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc
    18421 gtatcggtaa attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc
    18481 tggccgtttg cttcggcttc gtgctgggta gcttcatgct gtaatgacca atcgcggggc
    18541 gttcacgccc cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga
    18601 cctctctctt gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg
    18661 aataccgcgt ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta
    18721 gccgccaaag tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc
    18781 tctctctatt actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa
    18841 actaccgtat ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa
    18901 accccggaac ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc
    18961 aacgcatctt tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg
    19021 acgcgccggg acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc
    19081 aaaaccaaca tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta
    19141 aacagatccg cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg
    19201 tctggcatga agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc
    19261 ccgtaaccat caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg
    19321 ctgatgccgg tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg
    19381 ccaacaacgc agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat
    19441 tgcccttttc cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg
    19501 tgctgccaat gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca
    19561 ctgccagagc attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg
    19621 cgctgggtct ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat
    19681 acgtgctcat tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt
    19741 ttactctaca actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac
    19801 agggcggctc acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac
    19861 catgcaccac cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt
    19921 ggctgggcgg cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa
    19981 ttaccgtctt tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa
    20041 ccgcgtttgg ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg
    20101 atatatgggg attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta
    20161 tcaccagcct acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag
    20221 cggcattggt tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc
    20281 aaactcagac gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc
    20341 tcgttgaagc caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct
    20401 gtaaaaagtt tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca
    20461 cggtcttact tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc
    20521 atcttaatgt ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc
    20581 cacaagcacg cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc
    20641 gccaaccgtg aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag
    20701 atgtactggg agaagccctg aaattattag aattacaagg gattgccaac accacgctgg
    20761 agatggttgc tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca
    20821 aagaggcaat cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc
    20881 gtcagctgat gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg
    20941 cgttatcgga gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta
    21001 cgttttatcc cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg
    21061 cctacgattt cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag
    21121 caaagcagat ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc
    21181 aggcggatgt cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc
    21241 gccagggtgg tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta
    21301 agcagttgaa tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg
    21361 cgccccgttg cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt
    21421 ggttcgattc cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt
    21481 ttcgtttcag gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa
    21541 ccagattctc cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt
    21601 cctgagcgat agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt
    21661 caaatgacgt catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga
    21721 ctgatgagat tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa
    21781 gactaacaat ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac
    21841 acatttttct ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc
    21901 agtaaaatcc tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc
    21961 ttgcattcac caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc
    22021 aattatcatt gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc
    22081 gcgttggcga atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac
    22141 ttacccttga gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag
    22201 tacttagcag ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg
    22261 ttgtgacgca gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc
    22321 ctgtctatat cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt
    22381 acagcgaaga agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg
    22441 ttcctgacac aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg
    22501 aacaatcccc agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt
    22561 gtcgttaggt atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat
    22621 gagcaaatcg cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag
    22681 ttgtaacagc tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt
    22741 ggcgacatca cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat
    22801 tgatgaaccg agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata
    22861 ccgtgctcaa caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat
    22921 gctggataaa cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact
    22981 ggagagttta tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca
    23041 gcaaattttg cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt
    23101 actgcatggc gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca
    23161 atcatcccca gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca
    23221 ttctcaacac cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat
    23281 tgttacactg ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc
    23341 tctggtaaaa ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga
    23401 aatgtcctgg ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg
    23461 ggaagcagct gatgcatatc tcactgcctt taatttacgc ccaggggcaa acacccttta
    23521 ctggattgaa aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa
    23581 atttctcgct tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg
    23641 agtttttgta tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt
    23701 tattacataa attt
//
LOCUS       DAZPEP010000050        23445 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZPEP010000050 DAZPEP010000000
VERSION     DAZPEP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23445
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.050"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..2064
                     /locus_tag="ACHUT9_001982"
     CDS             <1..2064
                     /locus_tag="ACHUT9_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFR1348461.1"
                     /translation="GYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNSG
                     LNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSVR
                     FRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITDL
                     QLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHSD
                     FVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGDV
                     FDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYDI
                     ADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLRR
                     ISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFASN
                     NTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQAG
                     ASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMTP
                     YRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQPL
                     TFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDESR
                     NYICQ"
     gene            2080..3099
                     /locus_tag="ACHUT9_001983"
     CDS             2080..3099
                     /locus_tag="ACHUT9_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFR1348462.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNANLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATIN
                     SKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFMLESNGS
                     PVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALI
                     NVKYD"
     gene            complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHUT9_001984"
     CDS             complement(3181..3519)
                     /gene="rcnB"
                     /locus_tag="ACHUT9_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFR1348463.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHUT9_001985"
     CDS             complement(3738..4562)
                     /gene="rcnA"
                     /locus_tag="ACHUT9_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFR1348464.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHUT9_001986"
     CDS             4683..4955
                     /gene="rcnR"
                     /locus_tag="ACHUT9_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFR1348465.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      5047..5143
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5178..5966
                     /gene="thiM"
                     /locus_tag="ACHUT9_001987"
     CDS             5178..5966
                     /gene="thiM"
                     /locus_tag="ACHUT9_001987"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFR1348466.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5963..6763
                     /gene="thiD"
                     /locus_tag="ACHUT9_001988"
     CDS             5963..6763
                     /gene="thiD"
                     /locus_tag="ACHUT9_001988"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFR1348467.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6828..7552
                     /locus_tag="ACHUT9_001989"
                     /pseudo
     CDS             6828..7552
                     /locus_tag="ACHUT9_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
     gene            7604..8350
                     /locus_tag="ACHUT9_001990"
     CDS             7604..8350
                     /locus_tag="ACHUT9_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFR1348468.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8324..9289)
                     /locus_tag="ACHUT9_001991"
     CDS             complement(8324..9289)
                     /locus_tag="ACHUT9_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFR1348469.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9286..10290)
                     /locus_tag="ACHUT9_001992"
     CDS             complement(9286..10290)
                     /locus_tag="ACHUT9_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFR1348470.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10287..11564)
                     /locus_tag="ACHUT9_001993"
     CDS             complement(10287..11564)
                     /locus_tag="ACHUT9_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFR1348471.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11821..12873
                     /gene="fbaB"
                     /locus_tag="ACHUT9_001994"
     CDS             11821..12873
                     /gene="fbaB"
                     /locus_tag="ACHUT9_001994"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFR1348472.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13192..14046
                     /gene="gatY"
                     /locus_tag="ACHUT9_001995"
     CDS             13192..14046
                     /gene="gatY"
                     /locus_tag="ACHUT9_001995"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFR1348473.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14075..15332
                     /gene="gatZ"
                     /locus_tag="ACHUT9_001996"
                     /pseudo
     CDS             14075..15332
                     /gene="gatZ"
                     /locus_tag="ACHUT9_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15342..15794
                     /gene="gatA"
                     /locus_tag="ACHUT9_001997"
     CDS             15342..15794
                     /gene="gatA"
                     /locus_tag="ACHUT9_001997"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFR1348474.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            15825..16109
                     /gene="gatB"
                     /locus_tag="ACHUT9_001998"
     CDS             15825..16109
                     /gene="gatB"
                     /locus_tag="ACHUT9_001998"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFR1348475.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            16113..17468
                     /locus_tag="ACHUT9_001999"
     CDS             16113..17468
                     /locus_tag="ACHUT9_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFR1348476.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            17516..18556
                     /gene="gatD"
                     /locus_tag="ACHUT9_002000"
     CDS             17516..18556
                     /gene="gatD"
                     /locus_tag="ACHUT9_002000"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1348477.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            18662..19435
                     /locus_tag="ACHUT9_002001"
     CDS             18662..19435
                     /locus_tag="ACHUT9_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1348478.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(19517..20416)
                     /gene="yegS"
                     /locus_tag="ACHUT9_002002"
     CDS             complement(19517..20416)
                     /gene="yegS"
                     /locus_tag="ACHUT9_002002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFR1348479.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
     gene            20822..21139
                     /gene="yegR"
                     /locus_tag="ACHUT9_002003"
     CDS             20822..21139
                     /gene="yegR"
                     /locus_tag="ACHUT9_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFR1348480.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            complement(21469..22830)
                     /gene="yegQ"
                     /locus_tag="ACHUT9_002004"
     CDS             complement(21469..22830)
                     /gene="yegQ"
                     /locus_tag="ACHUT9_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFR1348481.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(22977..23309)
                     /locus_tag="ACHUT9_002005"
     CDS             complement(22977..23309)
                     /locus_tag="ACHUT9_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFR1348482.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
BASE COUNT         6044 a         5492 c         6002 g         5907 t
ORIGIN      
        1 ggctatgttc caccggaaaa ctgggagcgg ggtattaatg cgttttatac ctcttattat
       61 gtgagtcagt attacagtga ctataaagcg tcgggtaata gtaagagtac atatgtacgt
      121 tttaacagcg ggttaaattt actggggtgg caactgcatt ctgatgccag tttcagtaaa
      181 acaaataaca atccaggggt gtggaaaagc aataccctgt atctggaacg tggatttgcc
      241 caacttctcg gcacgcttcg cgtgggtgat atgtacacat caagcgatat ttttgattct
      301 gttcgcttca gaggtgtgcg gttgtttcgt gatatgcaga tgttgcctaa ctcgaaacaa
      361 aattttacgc cacgggtgca ggggattgct cagagtaacg cgctggtaac tattgaacag
      421 aatggttttg tggtttatca gaaagaggtt cctcctggcc cgtttgcgat tacagatttg
      481 cagttggccg gtggtggagc agatcttgat gtcagcgtga aagaggcgga cggttcggta
      541 accacctatc tggtgcctta tgcagcggtg ccaaatatgc tgcaacccgg cgtgtcgaaa
      601 tatgattttg cggcgggtcg tagccatatt gaaggggcga gcaaacatag tgattttgtc
      661 caggcgggtt atcagtatgg ttttaataac ttattgacgt tgtatggtgg ctcgatggtt
      721 gcgaataatt attacgcgtt cactttgggg actggctgga atacacgcat tggtgccatt
      781 tccgtcgatg ccacgaagtc gcatagtaaa caagacaacg gcgatgtgtt tgacgggcaa
      841 agttatcaaa ttgcctacaa caaatttgtg agccaaacgt cgacgcgttt tggtctggcg
      901 gcctggcgtt attcgtcgcg tgattaccgg acatttaacg atcacgtttg ggcaaacaat
      961 aaagataatt atcgccgtga tgaaaacgat gtctatgaca ttgccgatta ttaccagaat
     1021 gattttggtc gcaaaaatag cttttccgcc aatatgagcc agtcattgcc agaaggttgg
     1081 gggtctgtgt cattaagtac gttatggcga gattactggg ggcgtagcgg cagtagtaag
     1141 gattatcagt tgagttattc caacaacctg cgacggataa gctataccct cgcggcaagc
     1201 caggcttatg acgagaatca tcatgaagag aaacgtttta atatttttat atcgattccc
     1261 tttgattggg gtgatgacgt ttcgacgcct cgtcggcaaa tatatatgtc taactcaacg
     1321 acgtttgatg atcaggggtt tgcctcaaat aatacgggat tatcaggaac agtagggagt
     1381 cgggatcagt tcaattatgg tgtcaacctg agtcatcaac atcagggaaa tgaaacgaca
     1441 gctggagcga atttaacctg gaacgcgccg gtggcaacag tgaatggcag ttatagtcag
     1501 tcgagtactt atcgacaggc tggagccagt gtttcagggg gcattgtcgc ctggtcgggt
     1561 ggcgttaatc tggcgaaccg tctttccgaa acgtttgctg tgatgaatgc gccaggaatt
     1621 aaagatgctt atgtcaatgg gcaaaaatat cgcacaacaa accgtaatgg agtggtggta
     1681 tacgacggaa tgacacctta tcgggaaaat cacctgatgc tggatgtgtc gcaaagcgat
     1741 agcgaagcag aattacgtgg caaccggaaa attgccgcac cttatcgcgg cgcggttgta
     1801 ctggttaatt ttgataccga tcagcgcaag ccctggttta taaaagcgtt aagagcggat
     1861 gggcaaccat taacgtttgg ttatgaagtc aatgatatcc acggtcataa tattggcgtt
     1921 gtcggccagg gaagccagtt atttattcgc accaatgaaa taccgccatc ggttaatgta
     1981 gcaattgata agcaacaagg actttcatgc acaatcacct tcggtaaaga gattgatgaa
     2041 agtagaaatt atatttgcca gtaatagtca ggtggttcta tggaaattcg cataatgcta
     2101 tttatattaa tgatgatggt tatgcctgtg agctatgcgg catgttatag tgagttatct
     2161 gttcagcaca atttggttgt tcagggggat tttgcactta ctcaaacaca aatagcgaca
     2221 tatgagcata attttaatga ttcgtcatgc gtgagtacaa atactatcac ccctatgagt
     2281 tcgtcggata ttattgttgg gctttataac gataccataa aattaaattt acattttgaa
     2341 tggaccaata aaaacaacat cacgttgtca aataatcaga ccagtttcac cagtggttat
     2401 tcagttacgg tgacacctgc ggccagtaat gcaaaagtga atgtttctgc ggggggcggc
     2461 ggttcagtga tgattaatgg tgttgcgaca ttatccagtg cttcatcatc gacacgcggg
     2521 agtgccgcag tacaatttct actgtgttta ttaggtggca agtcatggga tgcatgtgta
     2581 aatagctaca gaaatgcatt ggcacaaaat gctaatctga cattgtcata caacccgata
     2641 accacaacct gcaaaccgga cgatttatta attactttag acagtattcc cgtttcacaa
     2701 ttaccagcca caggtaacaa agcaacaata aatagtaaac aaggggatat tattctgcgt
     2761 tgtaaaaatt tattaggtca acaaaatcaa acatcacgga aaatgcaggt gtatttatca
     2821 agttctgatt tgttagccgg cagtaacacg atcctgaaag gtgcagaaaa taatggagtc
     2881 ggatttatgc ttgaaagcaa tggttctcca gtcacatttt taaatatcac tgataacagc
     2941 aaaggataca caaatttaaa agaaattgcg gcaaagtcaa aacttacaga tacaacggtt
     3001 tcaattccga taactgccag ttactacgtc tacgatacaa acaaagttaa atctggcgca
     3061 ctggaggcaa ccgcattaat caacgtgaaa tacgactaat aacaagcaag acgagcaagt
     3121 ggctaataat aaaaaataac aaggtgtctg catttccccc tcatgatgag ggggcttttt
     3181 ttagcgatga taaaaaatct caccgtcgta ggctttaatg attttaccgt cggtgtcgct
     3241 gatcaacacg tacgcgccac ccatataggt ccagtgcgtc ccggcatcag gcgcgggcag
     3301 gttacgtaac tgccactgct taatgttgta ctcatcggtg cggtacatct ctgggacggt
     3361 atcacctggt ttgaaatgct taaagtcagc aatgaaacta cttaactcat acttagaaat
     3421 tcccgaggta ttggtcgaac ccgcggtggc tggtgcggcc caggcggcac tggtaaccag
     3481 caaaagcaca cccagcaaca tcttattttt aatggtcata aactctccac tttggggatc
     3541 tgtaatatca gtcgcatatc tttcctgcca tacaatataa ctgacaagcg atattttgtt
     3601 agtgatccca tgatttcgta aaaagtgtaa atgaaaggat gccctaaaag catccttagc
     3661 gagcagagag tcagcggaaa tagctgattt aagaatcatc ctgggctgaa gataacatgc
     3721 aagcctaaag gattgagtta tcgcattatg cccatgaagc catgtacgcc catatacaca
     3781 ccgactaagc caatcaacag actggaaaaa tagggggcgc gtttagcgag agtgttaaat
     3841 ccgctccagc gttttgcgac ctgctgaacg ctgattgctg cgccaacgcc tacggtgaca
     3901 agcgttaacg ccaggccaat gctgaaactg acgaccagtg ttgcgcccag tgtcagggct
     3961 ttcaactgaa tgcatatcaa cagcacggta attgctgccg ggcaggggat aaggccaccg
     4021 gttaagccaa atagcaaaat ttgccagttg gtgacctctc taccatcaaa gcgtcgttta
     4081 atgtcattgg catgggctcg tgcatgggca tcctgatact cgccatgttc gtgatggtga
     4141 tgatgtccat ggtcgtggtg atcttcgtga tcgtgatgat gatgctcata atcatactcg
     4201 tgcatattct ccagccagtt gcgttcgccg cgccaggtac gccagaacat ccagaacgcg
     4261 gtgccaataa tgatcactgc ggaaatcagc tggagccacg gttctgctga ttgagcagta
     4321 aagcgcttgc tgatcaccat cccgccaaag gcaattaacc agaccactgc ggtatgcgaa
     4381 atagttgctg ccagtccgag catcaccgct tgtttaatgg tgcctttgat ggcgatgata
     4441 aacgccgcca tcatcgtttt tgagtgccct ggttctaggc catgaagcgc accaagtaag
     4501 atggcgctgg ggatgaagaa ccaggcgttt ccttgctgaa gaagagttgt aaattcggtc
     4561 atgataataa ttcttagtat taattcggca atctgattct actcccccca gtacctgata
     4621 ctacccccca gtagattaat agtgctatga tattttcata ttcttgttaa ccaggtgttg
     4681 ccatgtctca tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg
     4741 gccaggtcgt ggcgctcaag aaaatgctcg acgagccgca tgaatgcgct gcagttttac
     4801 aacagattgc tgctatccgt ggcgcggtaa acggtctgat gcgggaagtg attaaaggtc
     4861 atctgacgga acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg
     4921 tcgttctgaa ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc
     4981 cgtttttcct cgtttacaac gcgtgcgctg gacattacca tcctcctctg cgatttatca
     5041 tcgcaaccaa acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc
     5101 tgatctggat aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt
     5161 tatggcagga gcaaactatg caagtcgacc tgctgggttc agcgcaatct gcgcacgcgt
     5221 tacacctttt tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa
     5281 cctttaccgc taataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg
     5341 aagaggccag tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga
     5401 cgcagccacg cgctcaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac
     5461 cctggacgct tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg
     5521 aacttttatc ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag
     5581 ctggcattgc taatggtgga cggggagtgg ataccactga cgccgcagct aacgcgatac
     5641 ccgctgcaca aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg
     5701 attatgttac cgatggccat cgcgccgttg gcattcacgg tggcgatccg ttaatgacca
     5761 aagtggtagg aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag
     5821 gcgatacgct ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagagcgcg
     5881 cagtcgccag aagcgagggg ctaggcagtt ttgttccaca tttccttgat gcgctctggc
     5941 aattgacgca ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga
     6001 tccgagtggt ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta
     6061 tggttgctca gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta
     6121 tcgcattgag cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat
     6181 cgataccact aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg
     6241 gttgcaacgt tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg
     6301 cgacccactg ctttcacctt cggcagttgc cacgctacgc agtcgattat tgccacaggt
     6361 ttcattaata acgccaaact tgcccgaagc agccgccttg ctcgacgcgc cacacgcgcg
     6421 caccgaacag gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt
     6481 gctaatgaaa ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga
     6541 gggtgaacaa cggtttaccg caccgcgcat tatgaccaaa aacacccacg gtactggttg
     6601 tacactctct gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt
     6661 acaggaggca aaaagctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg
     6721 tcacggtatt ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg
     6781 ctttggttat tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa
     6841 ttaccagtcg aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg
     6901 caatttatcc gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact
     6961 acatccactt ctacggttat cgcccggtta aatctttcgc tattcgtatt cctgccagtt
     7021 tgcctttatt aaggcgacgg aaggcgaaaa gctggtggac ccctattttt cgcgtaactg
     7081 gcaactaagc cgcgaaaatg gcttgctgcg cggggcatat cattatttct ccccgtcggt
     7141 agcagcaccg gttcaggtgc gattatttct gcaaacggta gatttctcac aaggcgattt
     7201 ccctgcggta ctggacgtag aagagcgagg aaaattatcg gcaaaagaat tacgcaagag
     7261 ggtaagtcag tggctaaaaa tggtcgaaaa aagtacgggg aaaaagccga ttatttactc
     7321 aggagccgtt ttttatcaca cgaatctggc gggctatttc aatgaatatc cgtggtgggt
     7381 ggctcactac tatcaacgtc gtccggacaa tgacggcatg gcctggcgct tctggcagca
     7441 ttccgaccgt ggacaggtag atggcatcaa tggtccggtg gattttaatg tgtttaatgg
     7501 cacggtggag gagttgcagg gattcgttga tgggattaaa gaaacgcctt aaataagaag
     7561 cagtgatata ctcccaccag actccctggc aggaaaaaga caaatggaac aagcgcatac
     7621 ccagctgatt gcccaactga acgaacgtat tttagcggcg gataacacgc cgctttatat
     7681 taagtttgcc gaaacggtaa aaaatgccgt gcgcagcggg gtgctggagc atggcaatat
     7741 tttgcccggt gagcgtgatt taagtcagtt aaccggcgtt tcgcgcatta cggtgcgcaa
     7801 ggcgatgcag gcgctggaag aagagggcgt ggtgacgcgt tcgcgcggtt acggcacgca
     7861 gatcaacaac atcttcgaat actcgttgaa agaagcgcga ggtttttctc agcaggtggt
     7921 gttgcgcggg aaaaagcccg atacgctatg ggttaacaag cgtgtcgtga aatgtcccga
     7981 agaagtcgcg cagcagctgg cggtcgaagc aggaagtgat gtctttttgc ttaagcgtat
     8041 tcgctatgtc gatgaagaag cggtatcgat tgaggaatcg tgggttccgg cgcatttaat
     8101 tcatgatgtt gatgccatcg ggatttcact ttatgactat ttccgcagcc agcacattta
     8161 cccacaacgt acgcgttccc gcgttagcgc ccggatgccg gatgccgagt ttcagtcaca
     8221 tattcagtta gatagcaaaa taccggtgct ggtgatcaag caagtggcgc ttgaccaaca
     8281 gcaacggcct attgagtaca gcattagcca ctgtcgcagc gatctatacg tttttgtgtg
     8341 cgaggagtag ttcctcgcgc gttggcgcac aatcaccgcc ccgatgcccg acaacccacg
     8401 acgccactgc attgcccagt aatacggcat ccgccagtgg cagaccagag gccagcccgg
     8461 caagtacgcc acctgcatga ctgtcgcccg ccccaatggt gtctacaact tgcgttggaa
     8521 atgccggaat gcagccagaa gcgtcgttgc tgaaatacca tgcgccttct ttatcgagac
     8581 gaacgatcaa cggcgcggca aatttctcct gccattgctc gccaagtgtt gttatctctg
     8641 cggataaagc aaaacgttcg gcggcaatct cagcctcttg acgattgagc gacactaaag
     8701 gtcgacaggc catgatccgc gccagtaatg catccgggat atcgccaata cgtgggccaa
     8761 aatcgataaa cggcgtcacg tcttgcagct cttccagcca ttccactaac aattcgccgc
     8821 agggcgaggc cagttgataa ccggaaaaat agagcaggct gccaggcgca acggttaatc
     8881 gcgccagcca ctggcgattc cactgatttt ccacaccgct gaatgacata aaagtgcgtt
     8941 cgccatccgg ctcaaccagc gccagacacc agccgttatc accttcggcg ttatcgatca
     9001 ggctgattaa gccctctttt gccatccggt tgcgaataat ctccgcccac acgccctgac
     9061 cgagcggcaa ggcattaccc gcttcgatgc cgaggcgctt taacgccacg gcaatattca
     9121 gtgcgcagcc gccaacgtta acgctctgct gtttcagttc gatatcgcac ccacgccagg
     9181 ggagggcata agcgtcggcg atcacgtcaa tgaccgccgc gccgacgacc atcaccggct
     9241 gacgcgtggt taattcaggc agcagcgtgt gtaatcgagc gccgctcata ccgcctcccg
     9301 ttgttgacga tatttcgcca gcgctgtggc atagcggttg aaatcaagct gatttaccgc
     9361 atccagttcc gcctttaacg caggatcgat agcgttaacg ccatgcaacg cgccgcaaat
     9421 tgccgtcgcc atagcaccga tggtgtctgt gtcgccgcca aggttagcgc acaggacggc
     9481 gcagcgattc gggtcggttt gtgctagttc aaccagcgca atggcgcacg gaacggactc
     9541 aatagtgctg gtacctgcgc caacgacctg gtaaagctgt tcgctggcgg attcggtgcc
     9601 gtcggcattg cgcacaattt tcagcgcaat ctccagccgc gccgccagtg aggcgctgaa
     9661 cgtagtgatg cgtttttgct gtgcatgtcg cgcaattgaa ggcagggaat caacaatcgc
     9721 tgaccagctt tctccgtcaa tggcacgaga aatcgcccat gcgatgacta ccgcgcctgc
     9781 aaccgccaga tcggatttat gggtcgggct ggacgccagc gctacatcat caataaagga
     9841 atcaacatca cgcgccggaa gcaaacaacc taatggcgag acgcgcatcg ctgcgccgtt
     9901 ggtcacgccg ttgttttcca gttcagcaac gggtttaccg tcgcgaatgg cgttaagcgc
     9961 aatcttcgag gtcggaccta gtacgttttt gttaaaggcg tcgaaacgca gcgcccagtc
    10021 gagaatatta cgcccaatca gatccggatc gatcttgcct tcacgttcca gtaacgcatc
    10081 cgccagacac agcgccatcg aggtatcgtc ggtgaattcg gcgcggttaa aataacaggc
    10141 cgcgttattc tcctttggtc caggaagaaa acggtcaatc cagccaaagt gtgctttaac
    10201 gcggctgcgt ggccaaagct cggagggcat ccccatcgca tcccctaacg cctgcccata
    10261 aagagcaccg agaatacgtt ctgttttcat ttaacttccc cttgtgtcaa cgcaatatcg
    10321 cgatcatcga ccttgatagc cgtaatttcg ttgtcggatt cgcggaaaaa aatcatgaac
    10381 agcacggcga taatggcaat catcaccgcg ccgaaagtcc acatcccgga ccagttgaaa
    10441 gtcagtccgt ttaccggttc ctgataagcg aacatctttt ccatcatcac accgccaaga
    10501 cgatagccga gcaaactgcc gaagccctgg cagcagagcg tgatcagccc ctgcgcagcg
    10561 gtacgcatat gcacgggggc ttttttatcg acatagatgt aagcggtaac gtagtaaaaa
    10621 tcgtaactta cgccgtgaag caaaataccg aggaacagta acgcgtaggt gaaatattca
    10681 tccgtactac cgtaaataaa gaagccatag cggatcgcag cggtgaccag accaagcaat
    10741 aatacctttt tgataccaaa gcgtttagtg aaaaacggca atgccagcat aaagaagatt
    10801 tcagagaact ggccgagcgt catccagccg gtggcgtttt tcatgccaac ttcggtcaga
    10861 taaccgttgg caaagatgta atagaacgcc agtggcatcg caaacaggaa tgaacagaag
    10921 aaaaagacga ggaagttttt atcgcgcagc aggatcagcg catccaggcc gagcatgact
    10981 ttaatatcca ttttgccggt gctttttggt ggcgtgtcgg gcaggaaaaa cgcaaacaca
    11041 ccgagcagag cagaacttcc ggcggtaatc agcagcggga tgttagtcgg tgagatatcg
    11101 gcatacccca gtatttgcgg caagaaacca catgccagac cggaggcaat ccagccgata
    11161 gtgcccatca cacgaatgcg cgggaaatca cgctccacat ccggcacgtt ggcaaaagcg
    11221 atgctgttag tcagcgcaat ggtcggcata taggttagag agtaagccag cagtaacgga
    11281 aagaacccgg caaaagtggt ctgttgcgca gcgaaataca tcagcagcgc gcctgcgaac
    11341 atcaataccg ccagcacttt ttgcgccgag aaaaagcggt cagtgatgga gccaaccaga
    11401 attggcgaca ggatcgccgc aatggcggta caggcatacg accagccaat ttctccggca
    11461 ctaaaaccgc ttttacttaa ccacaaccac aatggcacaa accacgcgcc ccagataaac
    11521 cattcaacaa acatcatgaa cgacagcttt gctgttgttt tcatctttta attccttcat
    11581 gacaggtaat gtaaggtacg cgatgacaat accacttaac aatacctttt aaataccttt
    11641 gcggtgaaaa cttgaccgcg ctaacatttt ttctgatgaa tcgagccaac agaaaacgct
    11701 gaaaaaacat ccaaaagatg gaaaaactcg cccgtaacga ccaggattat gattgcccgc
    11761 aaaaggcggg ccaggacaat ccatagccga tatccaatcg gaatttacgg gagcatagta
    11821 atgacagata ttgcgcagtt gcttggcaaa gacgccgaca accttttaca gcaccgttgt
    11881 atgacaattc cttctgacca gctttatctc cccggacatg actacgtaga ccgcgtaatg
    11941 attgacaata atcgcccgcc agcggtgtta cgtaatatgc agacgttgta caacaccggg
    12001 cgtctggctg gcacaggata tctttctatt ctgccggttg accagggcgt tgagcactct
    12061 gccggagctt catttgctgc taacccgctc tactttgacc cgaaaaacat tgttgaactg
    12121 gcgatcgaag cgggctgtaa ctgtgtggcg tcaacttacg gcgtgttggc gtcggtatcg
    12181 cggcgctatg cgcatcgcat tccatttctc gtcaaactta atcacaacga gacgctaagt
    12241 tacccgaata cctacgatca aacgctgtat gccagcgtgg agcaggcctt caacatgggc
    12301 gcggtggcgg ttggtacgac tatctatttt ggctcggaag agtcacgtcg ccagattgaa
    12361 gaaatttctg cggcctttga acgcgcgcac gagctgggca tggtgaccgt gctgtgggcc
    12421 tatttgcgaa actccgcctt taagaaagat ggcgttgatt accatgtttc cgccgacctg
    12481 accggtcagg caaaccatct ggcggcaacc atcggtgcag atatcgtcaa acaaaaaatg
    12541 gcggaaaata acggcggcta taaagcaatt aattacggtt acaccgacga tcgtgtttat
    12601 agcaaattga ccagcgaaaa cccgattgat ctggtgcgtt atcagttagc taactgctat
    12661 atgggccggg ctgggttgat aaactccggc ggtgctgcgg gcggtgaaac tgacctcagt
    12721 gatgcagtgc gtactgcggt tatcaacaaa cgcgctggcg gaatggggct gattcttgga
    12781 cgtaaagcgt tcaagaaatc gatggctgac ggcgtgaaac tgattaacgc cgtgcaggac
    12841 gtttatctcg atagcaaaat tactattgcc tgatacgcct catacgaaca gcaccacccc
    12901 gtaggacgga taaggcgttt acactgcatc cggcaaccta tgcctgatgc gacgcggaag
    12961 cgtcttatca tgcctacata gcactgccac gtatgtcgga aaaggcgttt acaccgcatc
    13021 cggcataaaa acacgcgcac tttgctacgg cttccctatc gggaggccgt ttttttgcct
    13081 ttcactcctc gaacagtttt catattgtcg ttttgtgatc gttatctcga catttaaaaa
    13141 caaatcattt cattatattt tgaaatcgaa aacaagcgac aggatatgaa aatgtacgtg
    13201 gtatcgacaa agcagatgct gaacaacgca cagcgcggcg gttatgcggt tccggcattc
    13261 aatattcaca atctcgaaac gatgcaagtg gtggtggaaa ccgctgccaa cctgcatgcg
    13321 ccggtcatca tcgccggaac gcctggcaca ttcacttatg ctggtacaga aaatctgttg
    13381 gcgctggtca gcgcgatggc gaagcaatat caccatccac tggcaattca tctcgaccat
    13441 cacacgaaat ttgacgatat cgctcagaag gttcgttctg gcgtgcgctc agtcatgatt
    13501 gacgcctcgc atttgccttt tgcgcaaaat atttcacggg tcaaagaggt ggtggatttt
    13561 tgccatcgct ttgatgtcag cgtcgaagcg gagctggggc aacttggcgg ccaggaagat
    13621 gatgtgcaag tcaatgaagc cgatgttttt tataccaacc ccgctcaggc gcgtgaattt
    13681 gccgaggcaa ccggaattga ttccctggcg gtcgccatcg gcacggctca tgggatgtat
    13741 gccagcgcac cggcgcttga tttttctcga ctggagaaca ttcgccagtg ggtgaactta
    13801 ccgctggtgc tgcatggcgc gtcagggtta tcgactaagg atattcagca aaccatcaaa
    13861 ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa atgccttctc gcaggcgtta
    13921 aaaaattacc tgaccgagca ccctgaagcg accgatcccc gggattattt gcagtcggct
    13981 aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg attgtggctg cgagggcagg
    14041 gcataacgca cctgccattt aacaaggaaa aaacatgaaa acgttaattg cccggcataa
    14101 agctggtgaa catatcggca tatgttcagt ctgttctgcc catccgttgg ttatcgaagc
    14161 ggcgctggca tttgatcgca acagcacgcg caaagtgctg attgaagcaa cgtcaaacca
    14221 ggtcaatcaa tttggcggtt ataccggaat gacaccggca gactttcgcg aatttgtttt
    14281 tacgattgcc gataaagtcg gatttgcacg cgaacgcatt attctcggcg gcgatcatct
    14341 ggggccaaac tgctggcagc aagaaaatgc ggatgcggcg atggaaaaat ccgtcgagct
    14401 ggtaaaagca tatgttcgtg ctggcttcag taaaattcat cttgatgcgt caatgtcctg
    14461 cgcgggggat cccataccgt tagcaccaga aacggttgcg gaacgagctg ctttgctttg
    14521 ctgcggaaag tgtggcgaca gattgccagc gtgagcaact gagctatgtc attggcactg
    14581 aagttccggt tccgggcggt gaggccagcg ccattcagtc agtacacatc acccatgttg
    14641 aagatgccgc caatacttta cgtacgcatc aaaaggcctt tattgcccgt gggctgacag
    14701 aggcgttaac acgcgtgatt gccatcgtgg tgcagccggg cgtggaattt gatcacagca
    14761 atattatcca ttatcagccg caggaagcgc agccgctggc gcaatggata gaaaacaccc
    14821 gaatggttta tgaagcacat tctaccgatt accagacccg gacggcttat tgggaattag
    14881 tccgcgatca ctttgcaata ttgaaagtcg gtcccgcatt aacctttgct ttacgcgagg
    14941 cgatatttgc actggcacaa attgagcagg aacttatcgc ccctgaaaat cgcagcggtt
    15001 gcctggcggt aattgaagaa gtgatgctcg acgaaccgca atactggaaa aaatattatc
    15061 gtacgggttt taacgattca ttactggata ttcgttacag cctgtcggat cgtattcgtt
    15121 attactggcc gcatagtcgg attaaaaata gcgtcgaaac gatgatggtg aatcttgaag
    15181 gcgtggacat cccactgggc atgattagtc agtatcttcc caaacaattt gaacgcattc
    15241 agtccgggga attatcagca ataccgcatc agctgattat ggataaaatt tatgatgttt
    15301 tgcgcgccta tcgctacggc tgtgcggaat aaggacggta tatgactaac ctgtttgttc
    15361 gtagcggaat ttcttttgtc gatcgtagcg aagttttaac ccatatcggt aatgagatgc
    15421 tcgccaaagg tgtggttcat gatacctggc cacaggcatt aattgccaga gaagcagaat
    15481 tccctaccgg gataatgctt gagcagcacg ccattgcaat accgcattgt gaggcgattc
    15541 atgctaagtc gtcagccatt tatctgttaa ggccaacaaa taaagttcat tttcagcaag
    15601 cggatgatga taacgacgtg gaggtatcgt tggttattgc gttgattgtg gagaatccgc
    15661 agcagcaatt gaaactttta cgctgtttat ttggcaagtt acaacagccc gatatcgtcg
    15721 agacactaat cactcttcct gaaacccagt taaaggaata cttcacaaag tatgttttag
    15781 attcagacga ataaatccct ctgtaacaat aaaaaggact atttatgaaa cgcaagatta
    15841 ttgtcgcttg cggaggcgcg gttgcgacct ctacgatggc ggcggaagaa attaaagagt
    15901 tgtgtcagaa tcatagtatt cctgttgaat taatccagtg tcgggttaat gaaatagaaa
    15961 cctatatgga tggtgtgcat ttgatatgca ccactgccaa agtggatcgt agttttggcg
    16021 atattccgtt agttcacggc atgcctttta tttctggtgt cggtatcgaa gcattacaaa
    16081 ataaaattct gactatctta caggggtgac ctatgttttc agaagtcatg cgttatattc
    16141 tcgacctcgg ccctacggtg atgcttccga ttgtcatcat tattttttct aaaatattag
    16201 gcatgaaggc aggcgattgc tttaaagcgg gtctgcatat cgggattggc tttgttggca
    16261 ttggccttgt gattggctta atgctggatt ccattggtcc ggcggcgaaa gcgatggcgg
    16321 aaaatttcga cctgaatctg catgtggtcg atgtcggctg gccgggctct tcaccaatga
    16381 cctgggcgtc gcaaattgcg ctggtggcga ttccgattgc gattctggtt aacgtggcga
    16441 tgttactgac ccgtatgacg cgggtggtaa atgttgatat ctggaatatc tggcatatga
    16501 ccttcaccgg cgcgttgctg catctggcaa ccggttcatg gatgataggg atggcaggtg
    16561 tggtaattca cgcggcgttt gtttataagc tcggcgactg gtttgcccgc gatacccgaa
    16621 atttctttga gctggaaggt attgctattc cgcacggtac gtcggcgtat atggggccga
    16681 ttgcggtgct ggtcgatgct atcatcgaga aaatcccagg cgttaaccga attaaattta
    16741 gcgccgacga tattcagcgc aaatttggtc catttggcga gcctgtcacc gtgggttttg
    16801 tgatggggct gattatcggc atcctcgcgg gttacgatgt caaaggtgta ttgcagctgg
    16861 cggtaaaaac ggcggcagtt atgctgctga tgccacgggt gattaaaccc atcatggatg
    16921 gtttaacgcc catcgctaag caggcccgta gtcgtttaca ggcgaagttc ggcggtcagg
    16981 agttcctgat tggcctggat cccgcattac tgctggggca tacggcggtg gtatcggcaa
    17041 gcctgatttt tatcccgctc accattttaa ttgctgtttg tgttccgggt aatcaggtac
    17101 tgccgtttgg cgatcttgcc accatcggct tcttcgtggc gatggcggtc gcggtgcatc
    17161 gtggaaatct gttccgcacc ttaatctcgg gtgtcatcat tatgagcatc accctgtgga
    17221 tcgcgacgca aactattggt ttgcacaccc aactggcggc taatgctggg gcgttaaaag
    17281 ccgggggtat ggtggcttca atggatcagg gcggttctcc cattacctgg ttactgattc
    17341 aggttttctc cacgcaaaat attcccggtt tcattattat cggcgcaatt tatcttaccg
    17401 gtattttcat gacctggcgt agagcgcgtg gttttattaa acaagagaaa accgttctcg
    17461 cagaataatt tttacctgag ggggaggtta tcccccttaa caatcaggag tttttatgaa
    17521 atcaatggtg aatgatactg atggtatcgt gcgcgttgca gaaagcgtca ttcctgaaat
    17581 taaacatcag gatgaggtgc gggtaaaaat tgccagctcg ggcttatgtg gttccgattt
    17641 acccaggata tttaaaaatg gtgcacatta ttatccaata acgttaggcc atgaatttag
    17701 cggctatatt gatgcggtgg gatccggtgt tgatgattta caccctggcg atgcggttgc
    17761 ctgtgtgccg ttattaccct gttttactta tccagagtgt ttgaaagggt tttattccca
    17821 gtgcgcaaaa tatgatttta ttggctcgcg gcgtgatggt ggatttgctg aatatattgt
    17881 cgttaagcga aaaaatgtct ttgctctacc cacggatatg cctattgagg atggggcttt
    17941 tattgagccg attaccgttg gcctgcatgc ttttcattta gcgcaaggtt gtgagaataa
    18001 aaacgttatt attattggtg ccggaaccat tggcctgctg gctattcagt gcgctgtcgc
    18061 gctgggagca aagagtgtga cggcgatcga cattagctca gaaaaactgg cattggcaaa
    18121 atctttcggt gcgatgcaaa catttaacag tagcgaaatg agcgcgccgc aaatgcagag
    18181 cgttttacgc gaactgcgct ttaatcagct tatcctcgag acggctggcg taccgcaaac
    18241 tgtcgaactg gcggtagaga ttgccggtcc tcatgcccaa ctggcgctgg tgggcacgtt
    18301 gcatcaggat ctgcatttaa catcggcaac gtttggcaaa atattgcgta aagagctgac
    18361 ggttatcggc agttggatga actattccag cccttggccg gggcaggagt gggaaacggc
    18421 gagccggttg ctgacagaac gtaagttaag cctggagcca ttaatcgctc accgtggaag
    18481 ctttgaaagc ttcacccagg cggtgcgtga catcgctcgt aatgccatgc cgggcaaagt
    18541 gttgctcatt ccatgaaacc gcgggccagc gtgatgctgg cccggtattg tgcaaaacag
    18601 atcattcacc aatggtcccc cttcgtttac actagccaca attgaatatg ggtaaatgac
    18661 gatgaattca ttcgagcgaa ggaataagat catccaatta gtgaatgaac agggaaccgt
    18721 gcttgttcag gatctggcgg gagtatttgc tgcctcggaa gcgacaatcc gtgccgattt
    18781 gcgctttctc gaacaaaaag gcgtggttac gcgctttcat ggcggtgcgg cgaaaataat
    18841 gtccggtaat agtgaaaccg agacccagga agtcgggttt aaagagcgat ttcagctcgc
    18901 cagcgcgcca aaaaacagaa tagcgcaggc ggcagtcaaa atgatccatg aagggatgac
    18961 cgttattctc gacagcggaa gtacgacaat gcttatcgcc gaaggattaa tgaccgctaa
    19021 aaatatcacg gtgattacca acagtctccc ggcagcgttt gccctttccg aaaataaaga
    19081 cattactctg gtcgtctgtg gtggcacggt ccgccataaa acgcgctcga tgcatggttc
    19141 cattgccgag cgttcattgc aggatattaa tgccgattta atgtttgtcg gtgctgatgg
    19201 tattgatgcg gttaatggta ttacgacctt taatgaaggt tattcgatta gcggtgcgat
    19261 ggtcaccgcc gctaataaag tgattgccgt tctcgattca tcgaaattta accgtcgcgg
    19321 ttttaatcag gtactgccaa tcgaaaaaat tgacattatt attaccgatg atgctgtatc
    19381 agaggtggat aaactggcat tgcagaagac acgggtaaaa ttaattacgg tgtagtattg
    19441 ttctgtatgc cggataaggt atttacaccg catccggcat ttcggattct ccctggttca
    19501 agagagaata gtttgattaa cgcaataata gacaatctgg tggtaatcga caacgcaacg
    19561 ccgccggaag tatttcaata tgaaagtttt gtccgctcaa cggttcgcca tcaagattaa
    19621 aagtgatttc gtgtggggct tgtatatcaa accacgacga agcgccttcg ataatattcg
    19681 ggttatcttc gtcagatttt aaggttgata cgagagccgg aagaatttca tcgccggtaa
    19741 aaatgcgcag ttgcagcaag ccatcgttaa ttaacgcgtt cggacacaat tgctgaccgc
    19801 caccggcctg acgcccgtta ccaataccaa tgaccagggc gtcaccttgc cagtgaaagt
    19861 tttcaccgcg gatttcacaa cggtccggtt gcagagtatc catgcgcatt aagccatgaa
    19921 tgatgtaaga gacgccaccc agcgcggctt ttaatttttc cggcgtttct gtggtaatac
    19981 gcgtcccaaa tccgcctgtc gccatattaa taaaacaggt ttgtttgttg acctgcgcca
    20041 tatctatcgc aatggcgtta ccggcaattg ccagtttcag cgccttatcc agtgcctcag
    20101 gaatccctac actggtggca aaatcattgg cggttcctaa tggcaaaatt cccagcgcgg
    20161 gtatgccatc cccctcacac tgaatcaacg ccgtagaaac ttcattaatg gtgccatcgc
    20221 caccaccggc aatcaccgtt gcgacgccca acttccgggc ctcctctaca tatcgtgcgg
    20281 catcgccttt ctcccaggtg acccgcacat ggatcgtcat tccttcctca cgcaacagca
    20341 taattgcttc gcgcaagggt agattgtcag tacttttgcc attaagaatc agtaagctgg
    20401 cgggaaattc tgccatgatc gtatgtgcct ttatgattgg tctgtagata gtgtagagca
    20461 gaaaacaaaa ggtggagtca gaacaggatg aaagtcggag gataaaaagt cagttcaagt
    20521 atggaggatt cagtggttac caccaggagc ttgcaatggg gacagcgaat actaacggct
    20581 gccaccgata aatttcaaaa aagagcatat acctaatatt caactaaaca gtggcatctt
    20641 caatataata tattaaagcc cccatggagt taccctgaag ggcctcaatg tccgtaattc
    20701 ctacttatgt aggaaatgtt gtacagaaca tttattataa tcctattcaa ttataataat
    20761 catgccatta ttatatttaa acactagaga gtgtcgttgg tatttaatgg gggaaggtga
    20821 gatgaaaaag atagctgcta tatcattaat tagtattttt cttatatctg gttgtgctgt
    20881 gcataatgat gaaacaagta tcggtaaatt tggtcttgca tataaaagta atattcagcg
    20941 taaactcgat aaccaatact acaccgaagc cgaagcttct ttagctaggg gcagaatatc
    21001 tggtgcagaa aatatagtaa aaaatgatgc agcccatttc tgtgttactc agggcaaaaa
    21061 aatgcagata gttgacctga agacagaagg tgcaggatta catggcgtcg ctcgtctgac
    21121 attcaaatgt ggagagtgag aatatttttt ggtaagcatc aaacatgcgc gttctggttg
    21181 tgcttagccg gaacctgtgc gagcacgatg ccgttacgtg aaaggcatcg tgctatgaag
    21241 ggagattcta tcgatgtggt caatggaaga cggtgaccag ggatagggct tatgcataaa
    21301 aaataagccc gtgtaaggga gattacacag gctaaggagg tggttcctgg tacagctagc
    21361 attttatggg ttatgttttt cagcgaaacg gatgataacc ttaataaatg cagctgtatg
    21421 tgatcggttt ctaagaattt tccatccggg aaaaataatc gaaattaatc acttaccgtg
    21481 tgggttacgc gtggtttccc cggagaaatt acgcatcagc agcgcgtaat ttagctcaag
    21541 atcctgcggg accgggagcc acacagtata accatcgcct ggtgcgaccg gcatcgcttc
    21601 acctttggcg ttttccatgt gctcaagggt aaagttaatg ttgccttgcg gcgtcatcag
    21661 ctcaaggctg tcgccaacgg agaatttatt tttcaccgct accgctgcga ggtccccctt
    21721 gcgctcaccg gtaaactcac caacaaactg ctggcggtca gaaactgaat aaccgtattc
    21781 gtagttctga taatcgtcgt gagtatgacg acgcaggaaa ccttcggtat agccacgatg
    21841 cgccagacct tccagagttt ccagcaggct ggtatcgaac ggtttgcccg cagcggcatc
    21901 atcgatagct ttacgataaa cctgtgcggt gcgtgcgcaa tagtagaaag atttagtacg
    21961 gccttcgatt ttcagcgaat gcacgcccat tttggtcagg cgttctacat gggcgatggc
    22021 gcgcagatct ttcgagttca tgatgtaagt gccgtgctca tcttcaaacg cggtcatata
    22081 ctcgcccgga cgctgggctt cttcgatcat aaacactttg tcggttggcg cgccgatacc
    22141 tagcgtcggc tcaacatttt gcaccggaat cggctcgtac ttgtgtacga tgttgccaac
    22201 gtcatcttct ttcccttcct ggacgttgta ctcccagcgg caggcgttgg tgcaggtacc
    22261 ctggttcgga tcgcgcttgt tgatatagcc agagagcagg cagcgaccgg agtaggccat
    22321 gcacagcgca ccgtgaacga agatctcaat ctccatatcc ggcacctgat tgcggatctc
    22381 ttcaatctct tccagcgaca gctcgcgaga gaggatcacg cgggtcaggc ccatttgctg
    22441 ccagaatttc accgtcgccc agtttacggc gttagcctgc accgagaggt ggatcggcat
    22501 ttcagggaag tgctcacgca ccagcataat cagccctgga tcggacataa tcagcgcatc
    22561 cggccccatt tccaccaccg gtttcaggtc acggataaag gttttcagct tggcgttgtg
    22621 cggtgcaatg ttgaccacga cataaaactt tttccccagc gcgtgggctt cattgatgcc
    22681 gagctgaaga ttttcgtggt tgaattcgtt gttgcgcaca cgcagggagt aacgcggctg
    22741 gcccgcataa acagcatctg cgccataagc gaaagcgtaa cgcatatttt tcagcgttcc
    22801 cgccggggaa aggagttccg gtttaaacat aattctctcg ttctgatgac aggtcagctc
    22861 cgcttcacct gatgaagcgg ttaggggagt gcccccactt taagggcggg cattgtagcg
    22921 ctgtgggggg atctggtaaa gcgccggacg ccgacaaccc acgcccggca tcgtaattac
    22981 gtattgtctt tcaccgtctg gcttgtgcca ttggctttaa cggaggcaat tccggtttca
    23041 cgagattgcg cggtggcgta catctggctg gagccgataa tttgatgatt ggcggctttc
    23101 agattgaaat agaatttgcc gttacttgcc gtttttttct catagcgttc ttcttgcggg
    23161 ctgttgctac gcaccgacgc gatgcccttt tccgcagagg cttttgatgt atacagctcg
    23221 ctggtgagga tagtctcacc attgcccgct tttagcacaa accggaactg attatcactg
    23281 ctcttactga gttcaaacca accagccata atgactcctt gattgaaatt gcgtatcgaa
    23341 aacagcatct aaaggtgctg tttaccgatt aaagtatgag cgattttctg atgcctgcca
    23401 gggcggagag ataatgtcag ttggtaatga ctccaactta ttgat
//
LOCUS       DAZPEP010000051        23417 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZPEP010000051 DAZPEP010000000
VERSION     DAZPEP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.051"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUT9_002006"
     CDS             complement(<1..674)
                     /gene="focB"
                     /locus_tag="ACHUT9_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFR1348483.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHUT9_002007"
     CDS             complement(696..2708)
                     /gene="hyfR"
                     /locus_tag="ACHUT9_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFR1348484.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHUT9_002008"
     CDS             complement(2738..3151)
                     /gene="hyfJ"
                     /locus_tag="ACHUT9_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFR1348485.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHUT9_002009"
     CDS             complement(3144..3902)
                     /gene="hyfI"
                     /locus_tag="ACHUT9_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFR1348486.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHUT9_002010"
     CDS             complement(3899..4438)
                     /gene="hyfH"
                     /locus_tag="ACHUT9_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFR1348487.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHUT9_002011"
     CDS             complement(4448..6163)
                     /gene="hyfG"
                     /locus_tag="ACHUT9_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFR1348488.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHUT9_002012"
     CDS             complement(6153..7733)
                     /gene="hyfF"
                     /locus_tag="ACHUT9_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFR1348489.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHUT9_002013"
     CDS             complement(7738..8388)
                     /gene="hyfE"
                     /locus_tag="ACHUT9_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFR1348490.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHUT9_002014"
                     /pseudo
     CDS             complement(8400..9838)
                     /gene="hyfD"
                     /locus_tag="ACHUT9_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHUT9_002015"
     CDS             complement(9855..10802)
                     /gene="hyfC"
                     /locus_tag="ACHUT9_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFR1348491.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHUT9_002016"
     CDS             complement(10813..12831)
                     /gene="hyfB"
                     /locus_tag="ACHUT9_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFR1348492.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVVCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHUT9_002017"
     CDS             complement(12831..13448)
                     /gene="hyfA"
                     /locus_tag="ACHUT9_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFR1348493.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHUT9_002018"
     CDS             complement(13701..14171)
                     /gene="bcp"
                     /locus_tag="ACHUT9_002018"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFR1348494.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14171..14743)
                     /locus_tag="ACHUT9_002019"
     CDS             complement(14171..14743)
                     /locus_tag="ACHUT9_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFR1348495.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14889..15767
                     /gene="dapA"
                     /locus_tag="ACHUT9_002020"
     CDS             14889..15767
                     /gene="dapA"
                     /locus_tag="ACHUT9_002020"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFR1348496.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15784..16818
                     /gene="bamC"
                     /locus_tag="ACHUT9_002021"
     CDS             15784..16818
                     /gene="bamC"
                     /locus_tag="ACHUT9_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFR1348497.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17031..17744
                     /gene="purC"
                     /locus_tag="ACHUT9_002022"
     CDS             17031..17744
                     /gene="purC"
                     /locus_tag="ACHUT9_002022"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFR1348498.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17912..18760
                     /locus_tag="ACHUT9_002023"
     CDS             17912..18760
                     /locus_tag="ACHUT9_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFR1348499.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHUT9_002024"
     CDS             18775..20790
                     /gene="tmcA"
                     /locus_tag="ACHUT9_002024"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFR1348500.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRGSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHUT9_002025"
     CDS             20864..21562
                     /gene="ypfH"
                     /locus_tag="ACHUT9_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1348501.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21672..21872)
                     /locus_tag="ACHUT9_002026"
     CDS             complement(21672..21872)
                     /locus_tag="ACHUT9_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFR1348502.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHUT9_002027"
     CDS             complement(21900..23027)
                     /gene="dapE"
                     /locus_tag="ACHUT9_002027"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFR1348503.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23031..23387)
                     /locus_tag="ACHUT9_002028"
     CDS             complement(23031..23387)
                     /locus_tag="ACHUT9_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFR1348504.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         5859 a         6225 c         6270 g         5063 t
ORIGIN      
        1 aaatggcgaa tggcaatggc gaacggaatc acaaacaaat tggcgataca gtgctcaaac
       61 ccactggcga caaacagggt gatgggcaaa atcatggcga cgattttgtc gcataatgaa
      121 cgcccgcaat aactcatcca cagcgccaga caaaccatca gattgcacat aatgccgagg
      181 ctgacagatt cagtaaatgt atgatgcatt ttgccctcgg cgcagtgtaa aaccgcgact
      241 ccccacatcg cgttttcact catcaccagc ccggaaaacc agattaacaa actgaaacag
      301 gcaatacctg ccagattacc gcaggccacc agaagtgcgt taatcagcca agttcgccaa
      361 ctaataacgc cccgactttt tgccatcacc gtcattaccg acgaggtgaa caggctggtg
      421 ccacaaaccg ccagcaaaat aaagccgagt gtaaagcaaa ggccgcccac cagatgggtt
      481 aatgcctgtg aagacggggc atcggcgata acggaaaggt aaaaagtaaa tccaatcgcc
      541 ataaacacgc cagcggacat cgccattaaa aagaccgaca ctttgctgcg ggcaatttta
      601 tgggcagtaa tccgttcagc gattgccgct tttctggcgc tcaactgcaa gtcgaaagag
      661 agtttgtttc tcatgcatac tcctgcaaaa gcagattaca acacctcgcg aaccgagagc
      721 cccagacgct gcattcgtga cagcagcgtg gtgcgcttca tccctaatcg tgtcgccgcg
      781 ccacggggcc cggcaacaat gccattggtt tctcgcaata cctgaacaat gcgctgacgt
      841 tcttcttcgt cattctctgg cgtcgtcggg tgcagcaact gcgccattga actgcgaagc
      901 gctgaatctt catttagcgt cggcagtaaa tggctttgtc ggacattaag atgtaaattc
      961 agactgttac cgcgagtcag cagtaccgct cgctcaatca cgttttccag ctcgcgcacg
     1021 ttgcccggcc agtcccacga catcaactgg cgtagcgcct cggtcgggat ggcgtcaatt
     1081 gaacgattca tatggcgcgc cattttttgc gtgaaatgct ttgctaaaag agggatatct
     1141 tccggacggt cgcgtagcgg cggcaattcc agtgggaaga cattcaggcg ataaaagaga
     1201 tcgctgcgaa actggcgatc ttcaaccatt tgccacaaat cacggttggt ggcggcaatg
     1261 actctgacat tcaccgggat cgttctactc ccgccgagac gctcaatctc ccgctcctgc
     1321 aatacgcgca gcagtttagg ctgaagttct aacggcagat cgccaatttc atcaagaaac
     1381 aacgtgccgc catcggcaat ttcaaaacga ccacgatggg tattaatcgc accagtaaac
     1441 gcccctttgt catgaccgaa taactcactt tccagaagac tggcggggat ggcagcgcag
     1501 ttgattttga ccagcggctt gtcgcgtcgc gggctaagtt gatggatcgc tctggcgatc
     1561 acctctttgc cggttccggt ttcaccacaa atcaacactg tactgtcgct cttcgccaca
     1621 atatctacct gctggagcag gtcttccatt gcctggcttt gatagataat gtcaccgacg
     1681 cccagattcg aaaggagctg ctcgctaagc tggtggtttt cttgctgcaa actttcctgc
     1741 aaatcggtca tgctacgcca ggcatcggca ttgccaacgg caatagcgat gcgatcggct
     1801 atgtgttgta gtagctgaca gttttcctca ctaaagagag tggaagaggt atgcgccagc
     1861 aacaatgcac ccggtgtatg gttgccaaag gtaagcggta taaggagcgc agattcgcag
     1921 ccgttagatg caagtaagag taataacggg tcgcgttgcc agagaaacag atcgtctgca
     1981 cggtgcgtca aggtcggttg tcgggtttgt agcgtttgtg tcagcaatac actgtcgcca
     2041 ggcatattgc gtggcagaca cgcacaatgt gaggcagaaa gatcgctgca ccacaggcta
     2101 aacttctcgt tctttcgatg atcgcccagt accatactga ctgaagccag accgaaaaaa
     2161 tgatggatct cacgagcgac gtcagcgatc agatcgtcga gatcaagatg tgacagcact
     2221 gcattagtaa tatcaaccag tacccgatga tgatcgcgat ctttctccag caaacgcagc
     2281 gccacctgct gttcttcatc attgagccat gattgcagaa ccggggtgat tatccgcgcc
     2341 agccagttaa acagtgtgca atcctgggga gtaaaacggc agccgtgggt gcgaataaag
     2401 cgtagctccg ccattccact actgccgaga aggacggcgt gacgcgaata atcgaccatc
     2461 tcgccctgat actctgttgt tgccgtcaca tcgctgccat catcatggta gcggtaatag
     2521 aagttgcttt tgagaacatg cagttcaata agttgtaccg ggctgaacgg cggcggcagc
     2581 gggatgaagg cgcgtaataa agacgcctta ccgtgtttct gcgctaaccg ctccgcgagc
     2641 attccgttta ccgcttcaat tgttattcct tgcggcgggg caaacatcgc ctcgtctgac
     2701 atagccatca acagtcctta ttaatgagaa atccttctca cgcaattttt ctggccatca
     2761 gatagatggc cggttcctgc acaatttcat ccaacagatg aatgaattga atgctacgcg
     2821 cctgccacac agacgtcgcg tcatccgcca gtggcgcgaa tgcccgggtc aacagggcgg
     2881 tgtggctggc gtcaataaca atctcaccaa aagtcatcac ccccagcatc ttacgtcggg
     2941 cttgctcctc ttcgaccagt gcaagccaat cttcgtattc cgtcagtggg caggggaagg
     3001 cgacattcag acaatcaatc acgccaacgt gatgaccgat agccagcgag taatacatta
     3061 cctgagagct gtgttccggc atctcgtcgc tactggcgac aaactttttt cgcagcgtcc
     3121 agaaaacaat ttccccgcac tcttcagtca tgtaaccccc ggagtacgcg aaagagttgc
     3181 tgaacgatac tattgagacg tggatcgtcg gcatcgcgca accaggtgtt aacccgattt
     3241 cctgtaggat cgtcgcttaa atggcgcagg agccgatcgc aaatttctcg cccctgacga
     3301 tagcccgcca gccgccgcgc ttctcgctca atggcgatac gctgtgatgg cgggatctgc
     3361 ggccacaacg gttgcatagt caccccggtg ggatcgcgat aatccaccgc gtgaatcttc
     3421 tgttgcagca aaccgagcgc cacggcgaaa ccgtgaatgg tggcggccgg tgttggcggg
     3481 cagccgggga tccaaacatc aatggggaca atggtgtcgc tgccgcccca gacgctgtag
     3541 agatcgtgga aaataccgcc gccgacaccg cacgcgccgt aggaaacaca aattttatga
     3601 tcgggggcag actcatacgc ccgaagtgca ggcatacgca tcgcccgggt gactgcgcct
     3661 gtaaataaca aaatatcggc gtgacgcggt gatgaaataa ccttaatgcc aaaacgttct
     3721 gcgtcgaata ctggtgtgat ggcggcaaaa atttcgattt cacaggcgtt gcagccgccg
     3781 caatcgacgc gataaacgta agccgagcga cggatatcct gtagcaaatg ctgcttcatc
     3841 ttttgcgttt gctcgtccag cgtgatgggc tggctgacat gttgtgtaag cactggactc
     3901 atagctgctc cttagccacc agcggtacat ctgtatcgtc gttgagcagc gtcgcgcgtt
     3961 gtttgcactc cgggcagacg ctcgcttgcg cccataacat ttcgcggttt tgtggcgcat
     4021 tttgttgctg tgccaacaat tcagcagcca gtgcgacggt tttttgcggg gcaaacgggc
     4081 gttcgcaacg gctgcaacgt tgtagatgga acgtcgcgcg ggtatagaga tcggctttat
     4141 tggtgacggt cagttcaaag ttattggtaa gctggatggc tctggtcggg cacacttctt
     4201 cacaacgtcc gcagtaaata caacgcctca gatagagctg ccaggtgcgc gaattttgct
     4261 ggtcgtcggt ctggatagtc agcgcatttg ccggacaagc acaggcgccg caggcaatac
     4321 attggctggg catcaggtcc ggtttcccgc gaaagcctgg gctgacctcc agtggcgcga
     4381 agggatattt caccgtcgcg gttccggcgc gcataatagt tttcagtaac ttcagcatct
     4441 tctgtcctta cttcagcggc gaacggttac gatcaatgcc gtagcgttcg atctctttat
     4501 acggcacggt ttttgactgg cgcttgcgca catctaccag cgtcacacgg tcggtacagg
     4561 agtagcaggg atcaaggcta ccgataatca gcggtgcgtc agaaacggta ttgccgcgca
     4621 gcatgtaacg caacaccggc cagttggcgt aggtggcggc acggcagcgc cagcggaaca
     4681 atttttggtt atcaccgagc atgctccagt gcacgtcttc accgcgtggc gcttcaacaa
     4741 agcccagcgc gaatgcgtga ggtttatagc taaagccttc ggtcagcagt ggggtatccg
     4801 gcatgttgtc gagggcaaat tccagcattg ccagcgaatc aaacgtctct ttgacacgga
     4861 ccatcacgcg ggagaaaaca tcgccgccgg taaaggtaaa cagtgttttt ggaatattgc
     4921 cgtagtcggc gtaggggtga tcaaagcgca aatcacgggc aaaaccactg ccgcggatca
     4981 gcggccctac agggctataa tcacgggcga tttgtcggtc gagaatgcca atgccctgag
     5041 tgcgttgttc catattcggc gtggcaagca gcatctctac cagctccgac acgtcggcgc
     5101 gcatctcgcg caccagtttc agcgtttgca gacgttgctc tttgagaata tcgcggcgaa
     5161 caccaccaat cagattcaga ccgtaggttt tacgcgaccc ggtcagcaat tccgccattg
     5221 tcatcgactt ttcccgcacg cggaaaaatt gcataaagcc ggtatcaaat ccaacaaaat
     5281 ggcaggagag gccaaggttg agcaaatgac tgtgcagacg ttcgacttcc agcagaatcg
     5341 agcgaatagt atgcgctcgt tgcggcacct caatccccag tgcattttca accgagttgg
     5401 tataggccac actgtgggca aaaccgcaaa tcccacacac gcgatccgat aagaaggtca
     5461 cttcgttgta gcccatccgc gtttctgcca gtttctccat gccgcgatgg acataaaaca
     5521 ggcggtaatc agcatcgaca atttgctcgc catccacaaa caagcggaag tgacccggtt
     5581 catcggaggt gatatgcagc gggccaatag ggatcacccg cgcatcgcta ttgccctcat
     5641 tgataaacgg atacgtttcg gtatcagtcg tcggttcagg gcgcagtcga taatccatcg
     5701 catctttgcg cagcggatgc atatcttccg gccagtcatc gggcaacacc aggcgacgct
     5761 gatccggcag gccaaccgga atcagcccgt acatatcgcg aatttctcgc tcgccccaga
     5821 ccgcggcagg gacgcgcggt gtgactgacg gaaactcccg actgtcggca tcgaccagtg
     5881 ccttcaccac gatccagcat ttttcggccc cttccattga aagggcataa taaaccgcgt
     5941 aatgaccgtt aagtgtccgc tcgtcgttgc caaacaggac tggaagccag ccatcatgtt
     6001 gataataaag ataatgtaca acgtcaggca gcagattgat tttcaccgta atggtgacct
     6061 gttcaggcgt ttgtcgctct tcatccagca ccgcgccggg gaactgcgtt ttcagggcgg
     6121 cgagaatcgc ttcgccacga tttgacgatg aattaacgtt cactgtgttt ctccgaaaca
     6181 gatgcggtgc ctgaaggcaa aaaatcatgc caggtgctac gttgtgcagg caggtcgtgc
     6241 gtccctgaga ggactatagt ggaagcgccc gccaggatcc tgatgacagg ttgtggaata
     6301 tgcgttccca tcgccagcat catgaccagc agaatcacca ttggcgaggt ggtcaaccag
     6361 ccgagatcac cccggttaac ggcctgcggc ggtttcgcca ttaacacccg cgctgccatc
     6421 cgtaccaggc ccgccagcac cagcgttaac agcaataaca gcaggacgat aatcagcagg
     6481 tgattacgtg ccagtccggc ggtaatggtc ataaattcgc taagaaaaat gttgaagggc
     6541 ggcatccctg ccagcgccag cgcaccgccg ccaaacagca cggcggtaaa tggcatgatt
     6601 ttgagcatcc cacagacgac gttgagatcg cgcgtgccgt acttgagcag tacattgccg
     6661 gaaccgcaga acagcagcgt ttttgccaga ctgtggttta agatgtgcag cagcgcggca
     6721 aaaattccca gcggcccgcc aatgcctagc gccaccgcga ccagccccat gttctccacg
     6781 ctggagtacg ccagcagacg cttaatgtcc cgctgtacca gaatgaaaaa tgccgccacg
     6841 gcaaccgaca acatgccgaa gatgagcaac aaccggttgg ggaaatcgct gccgatggct
     6901 tggcaaataa tgatgtaata gcgaatcagc accaacagcg cgcagttcag caatacggcg
     6961 gagagcaggg cgctgaccgg actcggcgct tcactgtgag catccggcag ccaggcgtgc
     7021 atgggaaata gcccggtttt ggtaccaaag ccaattagca caaacacaaa ggccaacagc
     7081 attaatgttg ggtcaagcaa ggacgattgc ttaagaacct cgctccagaa tatcgccatt
     7141 tctgcctgcg gcataacgct ggcggcgttg gcgtatacca gcacggtacc gaacagacca
     7201 aaagcgacac caacagtaca aataatgatg tacttccatg ctgattccag cgatgaacgc
     7261 tgaccgtaaa tgcctaccag aaacgccgag cttaaggtgg tggcttcgat cgccgcccac
     7321 atcacaatca ggttattgct ggtaacaacc agcagcatgg tgaacaaaaa cagatggaag
     7381 aaaccgtagt aatcgcacag cgtaacgggt gaaagctcgc cgtgtgccac ttcatgacgc
     7441 atgtagccaa tcgagtaaac accggtgaga aagccaatca caccaagaat ggcgaggaac
     7501 aaaccgccca gaccatcaat atgaagccac agtcccgcag cgaatatttc tcctgcatca
     7561 gcggcagttt ggaccaccca gagtgccaga atcagcagca gtgtgatccc taagctatgt
     7621 aatacagtca ccgtacaagt cgcagagagc ccccgtttcc ggcaggcaaa acagagcagc
     7681 gaaaaaagca gcggcgtgag caggagtaaa gcgaacatca cagaataact catctcatta
     7741 tcccttcagc gcggtcaagt tgttcacgtc cagcgtgccg tgggtacgcc atatttttct
     7801 tgccagtaac accatcacaa tgacggcgaa gatggcgtcg gtagcgatac ctatttccac
     7861 cagttccggt gctcgccagg caagaagcgc cagcaccaga tgggagccgt tttccatcag
     7921 gcagtaacca aaaatttgcc gcaggatatt gcgctggctg acaatgcaca gcaggccaag
     7981 cagaaaatga cctaacgcta ccgccagcgc cggtttcagc ccggtagcca tcggtagctt
     8041 cacgggctga acgacaaatg cgcaaagcag gacaattaac gccgcgagca gtgccatcat
     8101 tgccggaccg aataacgctt tttccgggat gttctgggga atatttcgtg cagcgtaagt
     8161 catgattaac ggtaccagca gcactttggt gataaaggcg ctggcggacc agatcagcag
     8221 ttgctctgcg gcgaacaggc acgagagagt ggcgaaaata gacaccagca ccagcgactg
     8281 gcaggcgtaa aatccgcatg acaggcgata gcttttgacg ctaatcacaa acagcgatgt
     8341 cagcatcatc agtcccgcca gattatttac gatcatagaa ccggtcattc atcatctcct
     8401 tactgcaacc aggtcgcggc gattacgctg gaaatcagcg acatcacaat cagtacaatc
     8461 aacaccaggc gcattgaccc tggcagcggt gcggcatcgg cgacggcctc gctcggtttg
     8521 cccggcacga cgcgaccaaa ccagcgaata aaccaggcga aactggcgac cgattcaatc
     8581 atcagcagaa tcatggcggg cagcaggatc cagtactcca ctgacaacgc aaaaccgacg
     8641 gcaaacagcg ggaatttact gaagaagccg ttgaacggcg gcacaccggt aatcgccagc
     8701 gctgccacgc agaaacccac gcttggcaac ggcagggtgt gcaatacgcc acgcagacgc
     8761 ggcaacaagc gcgtgccgca gctgtaactc agcgcacctg ctacaaggaa aaacaggctt
     8821 ttagcgaacg cgtggttgac gatgtaggcg atgctaccct ccagcgccag ccgcgagccg
     8881 aagatggaga gcgacaagcc gaagaacatc cagccaagtt gagtgatggt cgaccaggcc
     8941 agcaaccgct tcatatcctg ctgtggcaaa tacatcagaa agccataaag aatggtgacc
     9001 agcgccatga ccatgccaac gccgccaatc acatgcggga tattgccgcc gtcgataatg
     9061 gcgcgggcaa aaatgtaaac gcccactttc accatcgatg cggcgtggag ataggcactg
     9121 atcggtgttg gcgcttccat tgcatccggt agccacgctt gcatcggtag ctgggccgat
     9181 ttcccccacg cggcaaacag gatgccgcca taaaccagat aacgtgcgtc gccgtgtaac
     9241 tcgctcatcg cgctaagcgc aaacgttccg gtctgcaaaa acagcgtggc ggcggcaaga
     9301 tacaacccca acgagccgat atgagtgata agtaacgctt ttagtgctga aagctgtgct
     9361 ttatcgctct ggtaataact gatcaacgcc caggagcagc cgcccgtaat ttcaaaaaac
     9421 aacaactgac cgagcagcgt cgaggagagt accagtcccg ccatcgcgcc gataaacacc
     9481 agcaaaaatg cgtaataacg attcgtgccg ttatgcgggt gttcgcgatt tttatccgtc
     9541 agataacccg tggagtagat cgtgaccagc aaaccgagaa acaccaccac aaacagaatc
     9601 agcgtactca tgcgatcaat gaccaagcca aacaatgcca catcccccac gttgaccaac
     9661 gtcaacgtga cggcaacttt atcggcctga taaaacgcgg agatcagcga caacatgcac
     9721 agcgtggtca gtgcggcgaa caaaaccccc attcggcggc ccgacgttgt ggcgaaaacg
     9781 aaacgaccag tgcgccgata aaaggcagca ttaacgtcgt cagagcaaga ttttccatat
     9841 tccgtcagtg ctccttacag accggttaac cagaagaccc atgcaagcgc agcaaggctg
     9901 aagccaagcc aggtcacatg gtgaatgagt aaaaaacgcc cgcgtgccag cgtgttttct
     9961 gcgattgagg ccagtacaaa aatcagcaaa accttgagca gcgtaacgac aagtgaagtc
    10021 agcaggcagg cgagagaaag ttcttgcgcg cgcccaaagg gcagaaacag ggccacaaac
    10081 agtgctgcca tcacgacctg tttcagcccc agcccccatt tcgctagcgc cagcccggca
    10141 ccggaatatt cggtcagcgg gccttcctgt aattcctgtt ctgcttcagc aacatcaaag
    10201 ggaatttttc ccatctcaat gaagcaggca aaaccacagg ccagtaacgc cagtacggtg
    10261 gttagcggcg agttccagcc cgttgccagc gtattgctga tcatctcgat atgcgtggaa
    10321 cctgctatca gcgccaatac cagcagtgag agaatgagca tgggttcgac cagaatgccg
    10381 agcgtcaact cgcgactggc accgactccc gcaaacgggc ttccggtatc cagccctgaa
    10441 agagcaaaga aaaaacgaaa cagggcaaga agatagataa gggtgatcag atcgccgccg
    10501 cccgcaaaag gggaaacggt aataaatagt ggtagggcca tcgccagcac cagcatgctg
    10561 ctgattaata cccacggcat caggcggaac atcagacctg aagatgtcgg cgcatcttcc
    10621 tggcgtttaa acagtttgtg gatatcgcga taatcctgcc agatccccgg cccgcggcgg
    10681 gagtgcatac gcgcgcgtat ctgccgggaa atacccgtaa aaagtggggt tagcatcagc
    10741 agaatcacgg cctgtgctaa cgcaaaaatg accagatatc cgtcgcaaag agtttgtctc
    10801 atggtgattt ccttagacgg caatagcgat tagcagcaca accagtgcgg cgaccacata
    10861 caggcaatag agacgaaagt cgccgctttg cagatgctgt atttctttgg ccagccgttg
    10921 ggtggcgctc acgatggggc ggatcacccg ctcatcccag aagggttctg tgctctgagc
    10981 cctggcggtg acgtgcgcaa gacctttatt tagcctcagc gtagggtcga gctgttgtcg
    11041 tagacgaaat agcgcagaaa agaccacacg cagcggctgc atcacgccat tgcctgacgg
    11101 ggccatcgca ttttcccagc cgtagccgca tgcccaggca tctcctgtgc gacgaaacgc
    11161 tgcgcggcgc gaacgacacc acagccagta aaggccaggc agcaaaggca tcgccagtag
    11221 taacagcaac agtaatgaag gagtgacccg tgtatgaaac gtgccgggta caagtgctat
    11281 tccgctggcg acagtgacgg gaggggtatt ggtaaacgca tgggcaatgt gcattatctt
    11341 cggtgccagc caactggcac taagcgcaat taatacgcag agtgccgcga gcagtagcat
    11401 ggcgacgatc atcgtatttg gcacttcctg tgcctcttca gcgtgtgtgc tgcgcggcgc
    11461 accacagaaa gtaataccgt acattttgac gaagcacact actgccagcc caccagtgac
    11521 tgccagcatt accatagcaa taggacccgc aagttgtagc gctacggctt ccacacgact
    11581 tagtgagaat agcgactgcc aggtgtacca ttcgctgata aaaccattca gcggaggaat
    11641 ggctgatatc gccaggcaac caatcaggca tgctgcggct gtccacggca tccgtttcgc
    11701 cagtgccccc attttttcca tgtcgtgggt atgcaaacgc gaaataatcg cacccgcgcc
    11761 gagaaatagc agccctttga aaagcgcatg gttgagcaga tgaaacagtg cgccgagcag
    11821 tccaaccacg gtgagcagcg ggtcgtgcag tgacagaccg accatcgcca caccgaccgc
    11881 cagcaaaata atgccgacgt tttcgacggt actccaggcc agcagccgtt tgatatcctg
    11941 ttccgccagt gcatacagca cgcccaggag cgcggagatt gcgccgatcg ccatcaccag
    12001 aatgccccac cacagcggca aacccgtttg cgccagcaga tccatcgcca ctttcaggat
    12061 gccgaaaata ccgattttga ccattacgcc agacatcaac gctgaagcgt gcgatggtgc
    12121 ggcagggtga gcgcgcggca accagctgtg caacggcatc atcccggctt tcgcgccaaa
    12181 accgaaaaag gccagcagga acaccgccga cgccagcccc ggagaaagtg aaagcgtgcg
    12241 gaaactggca aaatcgaggc tgccgctttc gcgccccatc agcaagaagg cgatcattat
    12301 cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg ccagcgttga tcgacgtttt
    12361 atcctgcctg gcaatgacca gaaaccagga agacagcgac atcatttcaa acagcacgat
    12421 gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc gatgcgatga aaatattcat
    12481 aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg cgcatataag tcaatgaata
    12541 gagagaacaa accaccacca gcaaggagat aaccagcacc ataaatgccg agaggctatc
    12601 cattcgcagg gttaaatcgg caaattcaaa aggcgtggca aaacgggcga ctaaagttac
    12661 gcctgcatgt aattgcgtaa ttccgctgat gatgccaatc acgccgccta ccagcgatgt
    12721 gatgccggaa agcttaatag ccagtctgtc cagaccgagt aaaaacagcg aagccagact
    12781 ggcaaacaga tagaggatca gcgaccaggt taataattgc agggcatcca ttagcgttgc
    12841 tcctgagtga gggaaagggg atcctccccg ccattggcaa accagcttgc ggcaaggcgc
    12901 tgtttttctt tcatctggcg ttgcaggcta tcgtcggtga tcagccgtaa cgcctgattc
    12961 gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat cacacaggtc gcatttcacc
    13021 gcgacggtct ggacacccgg ttcccagcgc agcaacgcat gttggggcgg cacgttttct
    13081 tcgccgtctt tgagtgagcc ctcagcctga aaaacatatt gcgcaggggc attcaccgga
    13141 cggcttcctg aagcgctgat tgcgccaaat gggcagacca cggcgcaaag cttgcagcca
    13201 atacagaggc tttcgttgag ttggatcgca tcatccctct gagagatggc attgaccggg
    13261 cagacctgca ggcaaggggc ttcctcacag tgatgacaca cgacaggggc agtgagtgtt
    13321 gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac cttgcgtttt atgcacgtcc
    13381 gaacaggcag cgagacaggt attacatcct gtacaccaca gtggttcggc caccacaaag
    13441 cggttcatgt cggctccttg aaaatgatat gaaacattgc atttcatatt gcataaagga
    13501 tgccattctg attaactcaa tatattcaat aagttatgta ttaactggat tctgaacggt
    13561 acaaaaatga tggtcattaa cgttcagcaa aaaatggatt cgacacccgt gtcgcagggg
    13621 aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg ctggccgcaa gcgcagccag
    13681 cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc agttcagcac aacgtcgtgg
    13741 tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt tgccatcagc gtcaatcagg
    13801 aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga aggacttttc accccagacg
    13861 ccgaattgtt cgcacacctg gtggtcctca tcagacagga gcgtaaagtt aagcagctct
    13921 ttttccgcaa aacgggagag tttttcgggt ttatcggtgc tgatacccag cacatcaacg
    13981 cccgcttttt tcaactcatc catgttatcg cgtaagccgc aggcctgtac ggtacagccg
    14041 ggggtcatgg ctttcgggta gaaataaacc agaacacgct gtccctggaa gtcggtcaaa
    14101 ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg gtgcgatatc accggctttc
    14161 agtggattca ttacttaact ccatcctgtt catcatgttg ggaataattg acgacgttaa
    14221 tactgccttg tgcattgagt tctgtacata gggctttgaa cgcttgctca atatttgctg
    14281 cgtccgcaga tgcggggctg tgggcggtta tctgaatatg caactgcgca gcccgttcat
    14341 tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt catatgatgc gcgtcgaaaa
    14401 gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac atcgacctga acccagacag
    14461 atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt catcacgatt aaaagatcca
    14521 gctcggcacc tttcaacggt aacgttgatt caatcagagt aatggcattc catgaaccgg
    14581 aaagcagcat gataaacgtg aactcttctc ccagcatcgc caggcgactg tcttcaatat
    14641 tacagccgca actactgaca tgacgggtga tggtgttcac aattccaggg cgatcggcac
    14701 ccaatgcagt gatcaccaga taatgttgcg atgacagtgt caaactggtt attcctttaa
    14761 ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc atacaacaat cagaacggtt
    14821 ctgtctgctt gcttttaatg ccataccaaa cgtaccattg agacacttgt ttgcacagag
    14881 gatggcccat gttcacggga agtattgtcg cgattgttac tccgatggat gaaaaaggta
    14941 atgtctgtcg ggctagcttg aaaaaactga ttgattatca tgtcgccagc ggtacttcgg
    15001 cgatcgtttc tgttggcacc actggcgagt ccgctacctt aaatcatgac gaacatgctg
    15061 atgtggtgat gatgacgctg gagctggctg atgggcgcat tccggtaatt gccgggaccg
    15121 gcgctaacgc tactgcggaa gccattagcc tgacgcagcg cttcaatgac agtggtatcg
    15181 tcggctgcct gacggtaacc ccttactaca atcgtccgtc gcaagaaggt ttgtatcagc
    15241 atttcaaagc catcgctgag catactgacc tgccgcaaat tctgtataat gtgccgtccc
    15301 gtactggctg cgatctgctc ccggaaacgg tgggccgtct ggcgaaagta aaaaatatta
    15361 tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa ccagatcaaa gagctggttt
    15421 cagatgattt tgttctgctg agcggcgatg atgcgagcgc gctggacttc atgcaattag
    15481 gcggtcatgg ggttatttcc gttacggcta acgtcgcagc gcgtgatatg gcccagatgt
    15541 gcaaactggc agcagaaggg cattttgccg aggcacgcgt tattaatcag cgtctgatgc
    15601 cattacacaa caaactattt gtcgaaccca atccaatccc ggtgaaatgg gcatgtaagg
    15661 aactgggtct tgtggcgacc gatacgctgc gcctgccaat gacaccaatc accgacagtg
    15721 gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt gctgtaaagt ttagggagat
    15781 ttgatggctt actctgttca aaagtcgcgc ctggcaaagg ttgcgggtgt ttcgcttgtt
    15841 ttattactcg ctgcctgtag ttctgactca cgctataagc gtcaggtcag tggtgatgaa
    15901 gcctacctgg aagcggcacc gcttgcggag cttcatgccc cggctggaat gattttgccg
    15961 gtgacctccg gtgattatgc aatcccggtg accaacggta gtggtgctgt cggtaaggcg
    16021 ctggacattc gtccaccagc ccagccgctg gcactggttt ctggcgcgcg tacccagttc
    16081 acgggcgata ccgcttcatt gctggtggaa aatggtcgtg gcaatactct gtggccgcag
    16141 gtggttagcg tgctgcaggc gaaaaactac accatcaccc aacgtgatga tgctggtcag
    16201 acactgacca ccgattgggt acaatggaac cgtctggacg aagacgagca gtatcgtggt
    16261 cgttatcaaa tctctgttaa gccgcagggt tatcagcagg cggttacggt taaactgttg
    16321 aacctggaac aggcgggcaa accggttgca gacgcggctt ccatgcagcg ttacagcacg
    16381 gagatgatga acgttatttc cgccggtctg gataaatctg ccactgacgc cgcgaacgct
    16441 gcgcaaaatc gtgcctccac cactatggac gtacaaagtg cagctgatga caccggttta
    16501 ccaatgctgg tcgtacgcgg gccgttcaat gtggtttggc aacgtctgcc agcggcgctg
    16561 gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc agggcaacat ggccgtaact
    16621 tataagccgc tgtctgacag cgactggcag gaactgggcg cgagcgatcc aggcctggca
    16681 tccggtgact ataaactgca ggttggcgat ttagataacc gcagcagcct acagttcatc
    16741 gatccgaaag gtcatactct gactcagagt cagaacgacg cgctggtagc tgtcttccag
    16801 gctgcgttta gcaagtaaaa atacagggct ggaatcatcc ggcccttttt tctgatatga
    16861 tacgcaaacg tgtgcgtcgg caggaaaacg cgattttagc ggtaattcgc acgaaatttg
    16921 tttgtcggac gtagttcgga taaggcgttc acgccgcatc cgacaaaaca tccggcacac
    16981 cagacagcaa aagattttaa aacgttaatt cacacccagg agtgataaag atgcaaaagc
    17041 aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag cacggaaaac ccggacctgt
    17101 tggtgctcga attccgcaat gatacgtcag caggggatgg cgcgcgcatt gagcagtttg
    17161 atcgcaaagg tatgttgaac aacaagttca actacttcat tatgagcaaa ctggctgaag
    17221 cgggtatccc gactcaaatg gagcgtctgc tctccgatac cgaatgtctg gtgaaaaagc
    17281 tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc tgcgggctct ctggtgaaac
    17341 gtcttggaat cgaagaaggt attgagctga acccgccgct gttcgatctg ttcctgaaaa
    17401 acgatgccat gcacgatccg atggtcaacg aatcttactg cgaaaccttt ggctgggtga
    17461 gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa agcgaacgac gtgctgaaaa
    17521 aactgttcga tgatgctggt ctgattctgg tcgacttcaa gctggagttt ggtctgtaca
    17581 aaggtgaagt ggtactgggc gatgagttct ccccggacgg cagccgcctg tgggacaaag
    17641 aaacactgga gaaaatggac aaagaccgtt tccgccagag cctcggtggc ctgatcgaag
    17701 cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga ctgatttttc tgttcatcat
    17761 cttgccgtgc agctggcacg gcaagacaac cgctctcgga atatatgcat tgaacgctca
    17821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    17881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    17941 caatgtcgaa gacaggcgta acagctccgg cggtccgtca atgggcggtc ccggttttcg
    18001 cctgccaagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    18061 tggtgttgat ttaaccgggt tgatgaccgg acagccggtt tcccaacagc aatcaacacg
    18121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    18181 ggaagacacc tggggacagc aattcgagaa gatgggtaaa acctatcagc aaccgaagct
    18241 ggtcatgtat cgtggaatga cgcgtaccgg ctgcggggcg ggccagtcca ttatgggacc
    18301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    18361 agacaaactc ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    18421 tcatcatgtg cagaaattgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    18481 gacgcaggcg gaagtaaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    18541 cggtgtctgg gggcataaca tgcagcagca aggcgatctg gaagaggcgc tgaacgcggc
    18601 gcaggccatc ggcgatgacc gtttacaaca gcaaagtcag gggcgagtag taccagacag
    18661 tttcactcat ggtacttctc agcaacgcta cagctggttt aaacgtggtt tcgacagcgg
    18721 cgatccggca caatgcaata cttttggtaa aagcatttaa cttccggggc aaggatggct
    18781 gaactgactg cgcttcacac attaacagcg caaatgaaac gtgaagggat ccgccgcttg
    18841 ctggtgttga gcggggaaga gggttggtgt tttgatcatg cgcttaagtt gcgtgatgcc
    18901 ttacctggcg actggctgtg gatttcgccg cagccagatg ctgaaaacca ctgttctccc
    18961 tcggcgctac aaactttact tgggcgcgag ttccggcatg cggtattcga cgcccgccac
    19021 ggctttgatg ccgctgcctt tgcggcactt agcggaacgt tgaaagcggg aagctggctg
    19081 gttttgttac tccctgtatg ggaagagtgg gaaaaccaac ctgatgccga ctcgctgcgc
    19141 tggagtgatt gccctgaccc tattgcgacg ccgcattttg tccagcatct caaacgcgta
    19201 cttacggcgg ataacgacgc tattctctgg cggcaaaacc agccgttctc gttggcgcat
    19261 tttacccccc gtactgactg gcaccccgcg accggcgcac cacaaccaga acaacagcaa
    19321 ctcttacagc agctactgac catgccgccg ggcgtggcag cggtaacggc tgcgcgtggg
    19381 cgcggtaagt ccgcgctggc agggcaactc atttctcgta ttgcgggcag tgcgattgtc
    19441 accgcgcccg caaaagcggc aacggatgta ctggcacaat ttgcgggcga gaagtttcgc
    19501 tttattgcgc cggatgcctt gttagccagc gatgagcaag ccgactggct ggtggtcgat
    19561 gaagccgcag ccatacctgc gccgttgttg catcaactgg tatcgcgttt tcctcgaacg
    19621 ttgttaacca ctacggtgca gggctacgaa ggtaccggac gtggtttttt gctgaaattt
    19681 tgcgctcgct ttccgcattt acaccgtttt gaactgcaac agccgatccg ctgggcacag
    19741 ggatgcccgc tggaaaaaat ggttagtgag gcactggttt ttaacgatga aaacttcacc
    19801 catacaccac aaggcaatat cgtcatttcc gcatttgaac aaacgttatg gcgaagcgat
    19861 ccagaaacgc cgttaaaggt ttatcagtta ttgtctggtg cgcactaccg gacctcgccg
    19921 ctggatttac gccggatgat ggatgcacca gggcaacatt ttttacaggc ggctggcgaa
    19981 aacgagattg ccggggcgtt gtggctggtg gatgagggcg gattatctca agaactcagt
    20041 caggcggtat gggcaggttt tcgtcgcccg cggggtaatc tggtggccca gtcgctggcg
    20101 gcgcacggca gcaatccact ggcggcgaca ttgcgcggac ggcggggcag ccggattgca
    20161 gtccatccgg cgcgtcagcg cgaaggcgtt gggcaacagc tcattgccag cgctttgcaa
    20221 tataggcctg gcctcgacta tctttcggtg agttttggtt acaccgggga gttatggcgt
    20281 ttctggcaac gctgcggttt tgtgctggtg cgaatgggta atcatcgtga agccagcagc
    20341 ggttgctata cggcgatggc actgttaccg atgagtgatg cgggtaaaca gctggctgaa
    20401 cgtgagcatt accgtttacg tcgcgatgcg caagctctcg cgcagtggaa tggcgaaacg
    20461 cttcctgttg atccactaaa caatgccgtc ctttctgacg acgactggct tgaactggcc
    20521 ggttttgctt tcgctcatcg tccgctatta acgtcgttag gttgcttatt gcgtctgcta
    20581 caaaccagtg aactggcatt accggcgctg cgtgggcgtt tacagaaaaa caccagtgac
    20641 gcgcagttat gtaccacact taaactttca ggccgcaaga tgttactggt tcgccagcgg
    20701 gaagaggccg cgcaggcgct gttcgcactt aatgaggttc gcactgagcg tctgcgcgat
    20761 cgcataacgc aatggcaatt ttttcactga ctccttcaat tttctcccat ggtcattgtg
    20821 agtcagcggc gtaaaattaa tgacatcagt taaggagttc gccatgaaac atgaccattt
    20881 tgttgttcaa agcccggata aacctgcgca acagttgtta ctgcttttcc acggtgtcgg
    20941 ggataacccg gtggcaatgg gggaaatagg cagctggttt gcgccgctat ttcctgatgc
    21001 gctggtggtg agtgtcggag gcgcggagcc gagcggtaat ccggcggggc gtcagtggtt
    21061 ttcggtgcag ggtattacgg aagataatcg ccaggcgcgt gtggatgcca tcatgccgac
    21121 gtttattgag atggtgcgct actggcagaa acagagcgga gtaggggcta acgccacagc
    21181 gctaatcggt ttctctcaag gcgcgattat ggcgctggag agcattaaag ccgaaccggg
    21241 ccttgcttca cgcgtcattg cttttaacgg acgctatgcg agcctgccgg aaactgcgtc
    21301 tacagccacg acaattcacc tgattcatgg cggtgaagat ccggttatcg atctggcgca
    21361 tgcggttgcg gcgcaggaag cgttaatcag tgccggtggt gacgtgacgc tggatatcgt
    21421 ggaggattta ggccatgcga tcgacaatcg cagtatgcaa tttgccctgg atcacttgcg
    21481 ttataccatt ccgaaacatt atttcgacga agcgctaagt ggcggtaagc ctggcgatga
    21541 tgatgtgatt gaaatgatgt aaaagacaga ttacctggcc tgataagacg cgtgagcgtc
    21601 gcatcaggca tcgttcacca ttgccggatg cggcgttaac gccttatccg gcctacggtt
    21661 gagcactcag cttacttctt cggttgatcc tttttcggcc agtcgtcgtc atcgtcccac
    21721 ttatcgttga aatcacgatg cgggggaagc tccggcttgt tggcgagaaa tttcttgtgg
    21781 tcgacgcgct tgagatcttt aatcacgttc agcaggacgc ctaccaaaaa gacaatcacc
    21841 agaatccacc aatatttagc cagccagtcc atgcttattt cctcttgcag aaccactcat
    21901 caggcgacga gctgttccat gatacgttga tacatacggg caagtagctg caggtcggca
    21961 gcgttcacac attcattaat tttatgaata gtggcattga ccggcccgag ttccaccacc
    22021 tgcgccccca tgcgagcaat aaagcgcccg tcggacgttc cgcctgtggt cagtagctgc
    22081 ggtttaattt cattatagtg ctcaaccgcg ttaacgaccg catccaccag tttaccgcgc
    22141 gcggtcaaaa atggctgccc ggaaagccac caatccaccg tatagcgcag ttgatgtttt
    22201 tcaagcaggg caagcacctg cgctttgatc atctcatcag tcagttcggt gctgaagcgg
    22261 aagttaaact gcacaaacag ttcacccgga ataacgttgt tactgcccgt tcccgcctga
    22321 atattggcaa tctgcatact ggtcgccggg aagaattcat tgccctgatc ccactcaata
    22381 gccactaatt cattaaggaa aggtgctgcg cgatgtactg gattgtcagc cagatgtggg
    22441 taggccacat gcccctgcac gccatgaatg gtaaggttgc aggttaacga tccgcgacga
    22501 ccatttttca ccacatcacc aaccacttcg atactcgacg gttcgccgac cagacagtaa
    22561 tcgagacgct cattacgcgc cattaacgct tcgacgactt ttaccgtacc gttgtgggca
    22621 ctggcttctt catcagaggt gatcagaaat gccagtcgtc ccgtatggtt gggatgttgt
    22681 gcgacaaaac gttctgctgc taccaccatc gccgccagcg agcctttcat atctgccgca
    22741 ccgcgcccga ataacatgcc atcacgaatg gtgggttcaa acggcggatt gatccaacga
    22801 tcggcgtcgc caggcggcac cacgtcggta tgcccggcaa aggccaatgt ttcaccttgc
    22861 ccacgccatg cccaaaaatt ctgcgtatcg gcaaagtcca tgcgttcaac ggtaaaaccg
    22921 atcgcctgca aacgatcaat caacaaagcc tggcatcctg cgtcatcagg actcagggaa
    22981 gggcggcgaa taagctgttg tgtcagctca ataaccgggc acgacataga ctacacctca
    23041 tggaaaaatt gctgataact ggaatcactg aaacccagca gcataggctt accgggcgcg
    23101 cagagcaatg gacgtttgat aattgcaggc atttcagtca ttaatgccgc cgctgaggcc
    23161 gcatcggtga ttttattgcg ggtggtttcg tccagtttac gccaggttgt accacgggtg
    23221 ttgagtaacg cctcccaacc taattcgttg ataaaaccgt tcaataattc gctgtccagc
    23281 ccatcgacgc ggtaatcatg aaaacgatag tcgatgttat tggcttccag ccaacggcga
    23341 gcctttttaa tggtgtcaca atttttgata ccgtaaagtg taaccataat gaatcctttt
    23401 tcgatttatg agaataa
//
LOCUS       DAZPEP010000052        23238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZPEP010000052 DAZPEP010000000
VERSION     DAZPEP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.052"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(41..241)
                     /gene="glgS"
                     /locus_tag="ACHUT9_002029"
     CDS             complement(41..241)
                     /gene="glgS"
                     /locus_tag="ACHUT9_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFR1348505.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            510..1139
                     /locus_tag="ACHUT9_002030"
     CDS             510..1139
                     /locus_tag="ACHUT9_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFR1348506.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1166..2827
                     /locus_tag="ACHUT9_002031"
     CDS             1166..2827
                     /locus_tag="ACHUT9_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFR1348507.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3066..3125
                     /locus_tag="ACHUT9_002032"
     CDS             3066..3125
                     /locus_tag="ACHUT9_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1348508.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3442..3501
                     /gene="ibsE"
                     /locus_tag="ACHUT9_002033"
     CDS             3442..3501
                     /gene="ibsE"
                     /locus_tag="ACHUT9_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFR1348509.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3621..5054)
                     /gene="hldE"
                     /locus_tag="ACHUT9_002034"
     CDS             complement(3621..5054)
                     /gene="hldE"
                     /locus_tag="ACHUT9_002034"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFR1348510.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5102..7942)
                     /gene="glnE"
                     /locus_tag="ACHUT9_002035"
     CDS             complement(5102..7942)
                     /gene="glnE"
                     /locus_tag="ACHUT9_002035"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFR1348511.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEEPLSAQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7965..9266)
                     /gene="ygiF"
                     /locus_tag="ACHUT9_002036"
     CDS             complement(7965..9266)
                     /gene="ygiF"
                     /locus_tag="ACHUT9_002036"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFR1348512.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9508..10128
                     /locus_tag="ACHUT9_002037"
     CDS             9508..10128
                     /locus_tag="ACHUT9_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFR1348513.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10192..11430
                     /gene="cca"
                     /locus_tag="ACHUT9_002038"
     CDS             10192..11430
                     /gene="cca"
                     /locus_tag="ACHUT9_002038"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFR1348514.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11593..12414)
                     /gene="bacA"
                     /locus_tag="ACHUT9_002039"
     CDS             complement(11593..12414)
                     /gene="bacA"
                     /locus_tag="ACHUT9_002039"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFR1348515.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12505..12873)
                     /gene="folB"
                     /locus_tag="ACHUT9_002040"
     CDS             complement(12505..12873)
                     /gene="folB"
                     /locus_tag="ACHUT9_002040"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFR1348516.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12978..13595
                     /gene="plsY"
                     /locus_tag="ACHUT9_002041"
     CDS             12978..13595
                     /gene="plsY"
                     /locus_tag="ACHUT9_002041"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFR1348517.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13608..14540)
                     /gene="ttdR"
                     /locus_tag="ACHUT9_002042"
                     /gene_synonym="dan"
     CDS             complement(13608..14540)
                     /gene="ttdR"
                     /locus_tag="ACHUT9_002042"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFR1348518.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            14747..15658
                     /gene="ttdA"
                     /locus_tag="ACHUT9_002043"
     CDS             14747..15658
                     /gene="ttdA"
                     /locus_tag="ACHUT9_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFR1348519.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15655..16260
                     /gene="ttdB"
                     /locus_tag="ACHUT9_002044"
     CDS             15655..16260
                     /gene="ttdB"
                     /locus_tag="ACHUT9_002044"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFR1348520.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16309..17772
                     /gene="ttdT"
                     /locus_tag="ACHUT9_002045"
     CDS             16309..17772
                     /gene="ttdT"
                     /locus_tag="ACHUT9_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFR1348521.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17815..18828)
                     /gene="tsaD"
                     /locus_tag="ACHUT9_002046"
     CDS             complement(17815..18828)
                     /gene="tsaD"
                     /locus_tag="ACHUT9_002046"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFR1348522.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19065..19280
                     /gene="rpsU"
                     /locus_tag="ACHUT9_002047"
     CDS             19065..19280
                     /gene="rpsU"
                     /locus_tag="ACHUT9_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFR1348523.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19391..21136
                     /gene="dnaG"
                     /locus_tag="ACHUT9_002048"
     CDS             19391..21136
                     /gene="dnaG"
                     /locus_tag="ACHUT9_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFR1348524.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21331..23172
                     /gene="rpoD"
                     /locus_tag="ACHUT9_002049"
     CDS             21331..23172
                     /gene="rpoD"
                     /locus_tag="ACHUT9_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFR1348525.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5654 a         6331 c         5907 g         5346 t
ORIGIN      
        1 tctaatacaa acactaaaac gggccatcag gcccgttata tcagtgctct aactccagct
       61 ctcttgcctg catcatctgt tggcaatacc aggcataacg tgcgcaaaac caggttctat
      121 gttcttcatc catgttacca gtaacggcaa gaatatcgac cgggcgacct tctgcgtgca
      181 ttctggcaaa actacgcgcc aggaaatcga aattatttaa agaattaaga ctatgatcca
      241 taagcacatc ctccggttat cgcatacctt ttaaggccat atgctttttc tgatttcagt
      301 attccccaat cagtgtagtg cctttaaaca taacgtgcgt aaatatattg tccccgatca
      361 cactttttag tgaaaaataa aaaactatat taaaaaactt tctcttccag aacttctcta
      421 acatgtgtta ttattaacaa agataaatgt aagcaaggaa actatcccaa agtaaatatt
      481 gcgtcactaa atggacattg gagtgacata tgattttatt cgccgactat aatacccctt
      541 atcttttcgc tatttccttt gtactgttga tcggcctttt agaaatttta gcgcttatct
      601 gcggtcacat gctctccggt gctcttgatg cacatcttga ccattataat tccataacca
      661 caggtcatat tagccaggca cttcattatc tcaatatcgg aagattgcca gctctcgtcg
      721 ttctctgctt gctggctggt ttttttggtc ttattggcat cctgttgcag catgcctgca
      781 tcatggtctg gcaatcacca ctgtcgaacc tgttcgtggt tcctgtcagt ttactgttta
      841 cgattattgc ggtgcattat accggtaaaa tagttgcgcc ctggattcca cgcgaccata
      901 gttcagctat tacagaagaa gaatatattg gtagcatggc attaattacc ggtcatcaag
      961 ccacttcagg taatccctgc gaaggaaaac tcaccgatca atttggccaa atacattatt
     1021 tactactgga gcctgaagaa ggaaaaattt tcactaaagg agtcaaagtg ttaattattt
     1081 gccgactttc tgcaacgcga tatttggcgg aaaacaatcc ctggccacaa atattgtaat
     1141 ttattaatca aaaggaaatt taataatgga tgatattgtt aattctgtgc cctcctggat
     1201 gtttaccgcg attattgccg tatgcattct gtttattatt ggaattattt tcgccaggct
     1261 ctatcgtcgc gcttcggcag agcaagcttt tgttcgtact ggtttaggtg ggcaaaaagt
     1321 ggtgatgagc ggtggcgcaa tcgtgatgcc gatctttcat gaaataatcc ccatcaatat
     1381 gaatactctg aagctggaag tcagccgctc aaccattgat agcctgatta cgaaagatcg
     1441 tatgcgtgtc gatgtagtag tcgctttctt tgtacgggta aaaccgtctg tagaaggcat
     1501 cgccactgcg gcgcagacgt tggggcaacg cacgctatca ccagaagact tacgtatgtt
     1561 ggttgaagat aaatttgtcg atgccctccg tgcaacagct gcacaaatga ccatgcatga
     1621 gttacaggat acccgcgaga acttcgtgca gggagtacaa aatactgtcg cagaagacct
     1681 atcgaaaaac ggtcttgagc tggaaagcgt ttcacttacc aactttaacc agacctcgaa
     1741 agaacatttc aatccgaaca atgcctttga cgccgaaggt ttaaccaaac tgactcagga
     1801 gacggagcgc cgtcgtcgcg aacgtaacga agttgaacag gatgtagaag ttgcggtgcg
     1861 tgagaaaaac cgtgatgcac tttcgcgcaa gttggagatt gaacaacaag aagcgtttat
     1921 gacgcttgag caggagcagc aggttaaaac ccgtactgcc gaacagaatg cacgtattgc
     1981 ggcttttgaa gctgaacgtc gtcgtgaagc agagcagacg cgaattctgg ctgaacgaca
     2041 aattcaggaa acggagatcg agcgcgagca ggccgtccgc tcaagaaagg ttgaggctga
     2101 acgtgaagtt cgtattaaag agatcgaaca gcagcaggtc accgaaatcg ccaaccagac
     2161 gaaatcgatc gctattgccg ccaaatcgga acaacagtcc caggcagaag cgcgtgctaa
     2221 ccttgcactc gcagaagcag taagtgcgca acaaaacgta gaaaccactc gccagaccgc
     2281 agaagccgat cgtgctaaac aagttgccct aatcgctgcc gcgcaggatg cagaaaccaa
     2341 agcggttgaa ctgaccgtgc gggcgaaagc agaaaaagaa gccgcagaga tgcaggcggc
     2401 ggctatcgtt gagttagccg aagctacacg taaaaagggt ctggcggaag cagaagcaca
     2461 acgtgcgctg aacgatgcta tcaacgtact ttctgatgaa caaaccagcc ttaaattcaa
     2521 actggccttg ttgcaggcgc tacctgcggt aatagagaaa tccgttgagc cgatgaagtc
     2581 cattgacggt atcaagatta ttcaggtcga tggtctgaat cgtggcagcg ctgcgggtga
     2641 tgcaaacacg ggtaatgtgg ggggcggaaa cctagcggaa caagcattat cagccgctct
     2701 ctcttaccgc acacaggcac cgctgattga ctccttgctc aatgaaattg gcgtttcagg
     2761 cggctcactg gcggcattga cttcaccctt aacctcaaca actcccgtcg ccgaaaacgt
     2821 agaataaatt ttcagctatg aacgtccctg gcacaaaaag ccggggattt tttatatctg
     2881 cgttccgcta aaaactgcaa atgatcaggc cgttgcaacg ttttgcgtga ccgctcgggg
     2941 aaggcaaaac tgcctctggg aaagcgttgc gcgaggtccg gcgctcatca acaatcgggg
     3001 agtcagcaaa gggctgaaac gggaaagccc ctcccgagga aggggcctta cagaaggaaa
     3061 gggttatgat gaagctcgtc atcatactga ttgtgttgtt actcgtaagt ttcgcagctt
     3121 attaacagcc aatcagaggg gggagaaatc ctccctcacc cttgttcctt tactttaggt
     3181 tgaaaaaaaa tcagcgtcaa gaaggtttca ttaacacata tccctggccc aaaaagccgg
     3241 ggatttttta tatctgcgtt ccgctaaaag gtgcaaatgc tcaggacgtt gcagcgtttt
     3301 gcgtgatcgc tcggggaagg caaaactgct tcagggaaag cgttgcgcgg ggtccggcgc
     3361 tcatcaacaa tcggggagtc agcaaggggc tgaaacggga aagcccctcc cgaggaaggg
     3421 gccttataga aggaaagggt tatgatgaag ttcgtcatca tactggttgt gttgttactg
     3481 ttaagtttcc cgacttacta acaactgatc agagggggaa gaaatcctcc cttacccttg
     3541 ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct acaaagcgag
     3601 atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt gatgatgtta
     3661 gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt ggcccagact
     3721 tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag taaatctggc
     3781 aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac ccagtcgacc
     3841 gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg gcgggaatcc
     3901 cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcagacg gtcacccagt
     3961 ttgcgagcat ttgccagata ggagacgtgc ccggcgtgca ggatgtcaaa gacaccgttg
     4021 gtcattacca ccttttcgcc gcgtttacgc gctgccgcta cggccagctt cagttcctct
     4081 tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc cagctcgatc
     4141 ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc attggcaaag
     4201 aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac gccaatcacc
     4261 gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat atgcagcggc
     4321 gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa cagagccgag
     4381 agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc ggtcttacat
     4441 ttaccgacaa cagcttcaaa ttccgagaga ttcggcgtta acagcgtagc gccgcggtag
     4501 cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt acgcgccagt
     4561 tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag caccagcgcg
     4621 ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc aacgccttcg
     4681 aaaccttctt cgaaatccag acggatcagc tgttggttgc gggaaagtac ccgtaatttg
     4741 gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac gtcggccaga
     4801 gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag gcgtgcatta
     4861 gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg ttcttcgatg
     4921 gtattcactt taaccacggg caccggcgct tccggcgaga tacggctgga gggaccgtac
     4981 cagtaacgat ccagcattac atcaccaacc accatcactc ctgcacgttc aaactctggc
     5041 agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat aataccatac
     5101 ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc tctgcggtga
     5161 agcaatcccc cgacacatgg cccggcaatt cctgtaatgc cagatgatga agttcatcgc
     5221 gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata atgtcgtttt
     5281 gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc ggtttttcat
     5341 gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt ccgccttcat
     5401 cagctttgat atcaaagcga tcgcgatgtt tattgccgag atgagcgcgc attttctcgc
     5461 gcatttcccg cacttccgtt tgcagagttc taccttcacg cggcagcgtc ataatctcgc
     5521 gacgcactgc gtcaaagtgc gcggtgagct gcggatcgcc gtacactacc cgcgcacgca
     5581 ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca aatgcttctg
     5641 tggaggtaac cagcattccc gccgccccgg acgggcgcag ccgcgcatcc acttcgtaca
     5701 aaatgccgga agaggttcgc gtgctgaaca ggtgcataat gcgttgcgcc agacgaagat
     5761 aaaactgtcg tccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt gggcaatcat
     5821 ggaggaagat caggtcaaga tcggaactgt agcctaactc ccagccgccc agcttgccgt
     5881 agccgaccac tgcaaaacca cgcccttcgc gatcgttgag gtgattcggc ttaccgtagc
     5941 gggcaaccat ttgaacccac gcctgctgaa cgacggcatc tatcatggct tccgccagcc
     6001 aggttaagtg atcgctcact ttcatcaccg gtagcgtacc agcgatatcc gctgcggcga
     6061 tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgc tcttcgtcat
     6121 cttccggcac gcgcagcaaa tactggcgca actcatcgcg gtaggcatcg gtcgccgtcg
     6181 gctggtaaag agtgtttgga tcgagcaatt catccagcaa taatggataa cgcgccagct
     6241 ggctggcaat catcggcgat gcggcacaca gagaaatcaa atggttaagc gccgcaggga
     6301 attcactgag caattctaaa taggtggtgc gggtaacaat ccccaccagc aaggcggtaa
     6361 tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc agatgcggca
     6421 tcagatggtc gagcacctga cgtcctcgcg gcccgatggt gcgcttatcc agctctttgc
     6481 ggaaatcgga aatcagcgtt agcacctgtt tgcgatcatc ctcgctaaga tgcgccagca
     6541 ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac tgttccgaca
     6601 gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac acccggcgca
     6661 cattgttcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca aagtccatcg
     6721 cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtctgggtt tgttcgtcgt
     6781 taatgctttg cagcaggttt tccagacgcc gcaggaacag atacgccact cgcaattgtt
     6841 cagcatcgtt ttcagaaagc agatgcagcg cagcaatggc gctgagcgtt ggcagtaaag
     6901 agcacgattg cagcgacggt tcgcgcccgc cgcgaatgag ctggaatacc tggacgataa
     6961 attcaatttc gcgaatacca cccgcgccaa gtttgatgtt gtcggtcaaa ccgcgccgac
     7021 gcacttcacg ggcaatcatt cctttcatgt tgcgcagcga ctgaatcacg ctgaaatcga
     7081 tgtaacgacg gaaaacaaac gggcgtagca tcgcacgcaa ctcgttagca tagacgcctt
     7141 cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg cgcccctgct
     7201 cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt tcgccaaacg
     7261 gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga tccagcactt
     7321 taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc cggcgtccgc
     7381 cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag gagaaattca
     7441 gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct tcgccctgcg
     7501 cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca cgcgccgcaa
     7561 caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca gtaaccagtg
     7621 ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc cgcagctcgc
     7681 gcattaaccc ggcttcgtca ctcacattac tgagcgcctc ctgcaaccat gcagcgtaat
     7741 gctgccattc atcggcctgc ggcggttggc tttccagttc cgtcaaccac tctcgatgcg
     7801 caattatgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc tgtgcactta
     7861 gtggttcctc ggctaaaggc tctggcagcc gctcaacaac ggtctgccag tactgctgta
     7921 acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg ttttccgctg
     7981 tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgctcaat ttcaatgcgt
     8041 tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc atgcgcgacg
     8101 acaggatcat agtaacccgc cagcaacagt attgagtcaa tgtcacgcgt caggcgtggc
     8161 aactggtcac ggtagagatc gcctaacggc tggcagaaaa cgcttttcag ttcagcggca
     8221 tggtgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctgatttt gccctgcgct
     8281 ttggcatcta aaaatggctg ccatgctttg cttaccaacc attcggtcaa cgccagcttc
     8341 gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga agcaatggtc
     8401 gcttcgcatt gggtcagcaa atcacgtaag tgagtgctcg ctttacgcgg cacaatacca
     8461 ccgaacagca tcagcgtatg acggaccagg ctaatggctg ccagcacctg ttctttcgcc
     8521 gcatcgttac cgcgtaccca cagttcttca tgatactgcc attgcgctaa cgccagctcg
     8581 agcgccgctt ccagcccctg ttctacatcg gcttttgccg caacatgcaa aatggtggtc
     8641 ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc cgctttgctc
     8701 aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt cagcaccgcg
     8761 cgcgtgtcgc cgctaagcag ttccagttcc agctcacaga taggttcagc aaattccccc
     8821 gctttcacct ccccctgatc gagagcgatt tcaattcggc tgccatcgac cgccaccagc
     8881 catttttcgc gataaaaatc ggtgctgaac agcggttgta cgcgggaagc gagatcggca
     8941 ggcaattcac cgttcggcca gacttccgag ggaaactgcg ccaggtcgag cgtcggttcg
     9001 ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct tcctgcaact
     9061 ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat atcgtgccta
     9121 cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg gtcatggtgc
     9181 tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg attaacaata
     9241 aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca cataagtgac
     9301 gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg cgccaaattg
     9361 ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga cctgacacag
     9421 aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta ctatcgttcc
     9481 actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg attaacttta
     9541 ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt ttctgacgaa
     9601 ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg cacggttaac
     9661 gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc ccaggtgaaa
     9721 gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga gccaagcctg
     9781 cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa actcaccaat
     9841 atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc gcagagcgac
     9901 agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct gattgtcgcg
     9961 cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac catcatcatg
    10021 caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg tctggtactg
    10081 ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat cgccttcttc
    10141 gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg cgtgaagatt
    10201 tatctggtcg gtggtgctat tcgggatgca ttgttagggc taccggtcaa agacagagat
    10261 tgggtggtag tcggcagtac gccacaggag atgctcgacg cgggctacca gcaggtaggc
    10321 cgcgattttc ctgtgtttct acatccgcaa acgcatgaag agtatgcgct ggcacgtacc
    10381 gaacggaaat ccggttccgg ttacaccgat tttacttgct atgccgcacc ggatgtcacg
    10441 ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca ggacgataac
    10501 ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct gttgcgccat
    10561 gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg ttttgctgcg
    10621 cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat gcgcgagatg
    10681 acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga gacggaaagc
    10741 gccctcacca cccgcaatcc acaggtgttc ttccaggtac tgcgcgattg cggcgcactg
    10801 cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcccctgc caggtggcat
    10861 ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc gatgctgagt
    10921 ccgcaggtcg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg actgacgccg
    10981 ccagaacttt ggccgcgtca tcatggtcac ggtccggcgg gtgtgaaatt agtggaacaa
    11041 ctctgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact ggtggctgag
    11101 tttcacgatc tcatccacac cttcccaatg ctgaacccaa aaactatcgt caaattgttt
    11161 gattccatcg acgcctggcg taaaccgcag cgtgtcgagc aactggcgct gaccagcgag
    11221 gctgacgtac gcggcagaac cggttttgaa tcagcggact acccgcaagg ccgttggttg
    11281 cgcgaagcct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga agcgggattt
    11341 aaaggtgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt agccagttgg
    11401 aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttaac acgaaccatc
    11461 ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc ggtgcgaaca
    11521 atcaccccca ccccggccct ctccctacag agcgagggag aaaaccaggc ctgctcccga
    11581 accttttctc aatcaaaaga acacgacgta caccgcagcc gccacaataa agcgataaat
    11641 ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa tcgctatcag
    11701 cgccaccaca aaagcggtga taaacccaac ggcaaacatc gagatatcgc cggttgtcag
    11761 gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg gtaccgccag
    11821 caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc cacctgaaat
    11881 ggtggccccg gaacgggaga aacccggcca cagcgccaga cactggaaac agccaatcat
    11941 aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt tcggcttcag
    12001 gcattcggcg gcaatcagca acaaaccgcc aacgaccagt gcatacatca catttatcgg
    12061 gttaaacaat gacttaatcg tgtcgtggaa caacagcccc agtaccaccg ccggaatcat
    12121 ccccagcaaa atgtggatca gcgttaaacg acctttgctt tctccttcgt gctgcaacgg
    12181 gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca ctactaccgc
    12241 cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc cctcgaaccc
    12301 caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa attctgtcaa
    12361 tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc tcatcaataa
    12421 acccctaaat tattaaaatg taccgcttgt ccggactact gcagtaagac caggttataa
    12481 ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc cacgctcaat
    12541 gattacacca acattcgccg cccgcgccat tgcgcctggc ttgctgagtt tgatgcgcac
    12601 ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca cgcgttccac
    12661 cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt cagcgtaact
    12721 gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg ccatttcgat
    12781 atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa tagtggtgat
    12841 taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt ttggctaacc
    12901 ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga acacgaattt
    12961 tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc gcgtacctct
    13021 gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc gatccgcgaa
    13081 ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc aagggagcag
    13141 ccgtagcagt actgattttc gacgttctga aaggaatgtt gcccgtctgg ggcgcgtatg
    13201 aattaggtgt cagccccttc tggttaggct taattgccat cgccgcctgt cttggacaca
    13261 tctggcccgt tttcttcgga tttaaaggag gaaaaggcgt tgctaccgct tttggtgcca
    13321 tcgcacccat tggctgggat ctcaccggag taatggcggg aacctggtta ctgaccgtgc
    13381 tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct ccgttttatg
    13441 tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc ctgatcctgc
    13501 tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa atctggacga
    13561 aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta catgacctga
    13621 tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa ttccacgcag
    13681 acacgtaatt tcatgctgcg atagagcggc tcccggtaaa cagcccagat attggcgctt
    13741 tgtgcatact ccggcaatac ttgcaccagt ttgccactct ccagaaacgg caacacatcc
    13801 cactcggaac gcagcataat ccctttgccc tccagcgccc attgcaacac tatctcgccg
    13861 ctattggagg aaagatgccc gctaaccttc accgattttt tctcctgacc gttccccaac
    13921 tcccatattc cgtgggtcat atcgcgctct ttggtcacca gacagtcatg acgacttaat
    13981 tcctgtaatg attgtggctg cggatatttt tgcagatact caggtgctgc gcataatatt
    14041 cttttatttt tcgttaacag atgcgcaata taataatcag gaatttcgtc attaatacga
    14101 atatccagat caatattatc ctgcactaaa tcaatttgcc gatcgaacaa ttcaaaatgc
    14161 acctgtaact caggataatt gcgcataagt tcggtaatgg ctggcgcaat atggctgcgc
    14221 ccaaaaccaa aactgcaacc aatacgaatc atcccctccg ggcgcgtttt gatttgcgtg
    14281 acgtcatcga ccagtcgctg atattgggtg aggatttcca gcgcatgttc gtaacaacgt
    14341 tggccacttt cagtcagcgc cacgccgcga gcggagcggt tcagcagcgt tgttgccagc
    14401 gtattttcaa ggatttggat ccgcttggtg acaaatgcag gcgtctgccc aagcgtcgcc
    14461 gctgcggcgc taaaactccc gctgtggacg atttctacca gtacctgcaa gtctttggct
    14521 aaaggccagc tattcagcat cgttaattat ccgcagttgt gataagcgca gtgtatttcg
    14581 caaaacattg ccattccctg ctctggcgca atattcattc ttattttcgc gttgattcac
    14641 gaattgttaa ttacatattc acggtggcaa aaaatataaa accacatttt tagtggtagt
    14701 ttgtggcggt gaatttttcc agacaaatac aaaaactgga gttgccatga tgagcgaaag
    14761 taataagcaa caggcagtga ataagttgac ggagattgtc gctaacttta ccgccatgat
    14821 ttctacccga atgcctgatg acgtggtgga taaactaaaa cagctaaagg atgccgaaac
    14881 gtcgtcgatg gggaaaatta tctaccatac gatgttcgac aacatgcaaa aagcgattga
    14941 cctgaatcgt cctgcctgtc aggacaccgg ggagattatg ttcttcgtta aagtcggttc
    15001 ccgcttccca ctgcttggcg agctgcaaag catactcaaa caagccgtgg aagaggcaac
    15061 cgtcaaagcg ccactacgtc acaatgcggt agaaattttt gacgaagtaa acaccggcaa
    15121 aaataccggt agcggcgtac cgtgggtcac ctgggacatc atccccgaca atgacgatgc
    15181 ggaaatcgaa gtttacatgg caggcggcgg ctgcacgcta cctggccgct cgaaagtgtt
    15241 aatgccgtca gaaggctacg aaggcgtggt gaaattcgtc ttcgaaaata tctccaccct
    15301 cgccgtaaac gcctgtccac cggtactggt gggcgtgggc atcgccacct cggtggaaac
    15361 cgccgccgta ctctcgcgta aagccatttt gcgcccgatt ggcagccgcc atcccaatcc
    15421 aaaagcggca gaactggagc tacgcctgga agaaggactc aaccgtctgg ggattggtcc
    15481 acaagggctg actggcaaca gttcagtgat gggcgtacat atcgaatctg ccgcccgcca
    15541 tccgtcaacc atcggcgttg ctgtctctac cggctgctgg gcgcatcgtc gcggcacact
    15601 gctggttcat gccgatctca cctttgaaaa tctgtctcac acccggagcg cgttatgaaa
    15661 aagatcctga caaccccgat caaagctgaa gatctgcaag atattcgcgt cgacgatgtg
    15721 atctacctga ccggtacgct ggtgacctgc cgtgacgttt gtcaccgccg cttgatcgaa
    15781 ctgaaacgtc cgatccctta cgatctcaac ggcaaagcga ttttccacgc tggccccatc
    15841 gtgcgcaaaa acggcgataa atgggagatg gtttccgttg gcccgacaac cagtatgcgt
    15901 atggaaagtt ttgaacgtga atttatcgag cagaccggcg tgaaactggt ggttggcaaa
    15961 ggcggaatgg ggccgctgac cgaagaaggc tgccagaaat tcaaggcgct acatgtgatt
    16021 ttcccggcag gctgcgcggt gctggcggca acccaggtgg aagagattga agaagtgcac
    16081 tggacagagc tcggaatgcc ggagtcgctg tgggtctgtc gggtcaaaga gttcggcccg
    16141 ctgattgtct ctattgatac ccacggcaac aacctgatag ccgaaaacaa aaagctgttc
    16201 gccgaacgcc gcgatcccat cgtggaagag atctgcgagc acgtccatta catcaaataa
    16261 ccctcccgga gaggctcgcc ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    16321 actgaatggt ggcgatacct tgcgccgctg gcggtcatcg ccattattgc tctaattccg
    16381 gttcccgcag ggctggagag tcatacctgg ctctactttg ccgtttttac tggcgtgatc
    16441 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatatccatc
    16501 atcgccatac tctctccctg gctgctgttc agcccggagc agctcgctca gccaggcttt
    16561 aaattcactg caaaatccct ctcgtgggcc gtttccggtt tttctaattc ggttatctgg
    16621 ctgattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    16681 attgcgctga ttctggtgaa aaagatggga catcgcacgc tgtttctcgg ctatgcggtg
    16741 atgttctccg agctgatcct cgcacctgta acaccgtcca actcggcgcg tggtgcgggg
    16801 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    16861 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    16921 agcgccattt tcttgacggc gatggcacca aacttgctgt taattggact gatgaaaagc
    16981 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagtatt
    17041 ttgctggtgc tgctggttcc ctggctggct tatgtgctgt acccgccggt actgaagtct
    17101 ggcgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    17161 cgtgaaaaac ggatgctggg gctgatagta ggcgcgctgg tgctgtggat tttcggcggt
    17221 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cactgatgct gcttctgcgc
    17281 attatcagtt gggacgacat tgtcagtaat aaagcggcgt ggaacgtttt cttctggctg
    17341 gcctcgctta tcaccctcgc taccggactc aacaacaccg gttttattag ctggtttggc
    17401 aaactgttag caggcagctt aagcggttat tcgccaacga tggtgatggt ggcgttgatt
    17461 gtggtgtttt atctgctgcg ctactttttc gccagcgcca cggcgtatac ctccgccctc
    17521 gcaccgatga tgattgccgc tgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    17581 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    17641 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    17701 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    17761 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    17821 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    17881 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    17941 ctccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    18001 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    18061 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    18121 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    18181 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    18241 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    18301 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    18361 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    18421 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    18481 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    18541 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    18601 cacagcatca atgtcttttg ccgttaagcc agactccttt agcgccgcct ggatcaacgg
    18661 tacggttttg cgcacgtgat cgcgggaggc cagttcaggc acgacgccgc catagtcagc
    18721 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    18781 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    18841 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    18901 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaggccgcg
    18961 gtgctttaca aagcagcagc aattgcagta aaattccgca ccattttgaa ataagctggc
    19021 gttgatgcca gcggcaaacc gaattaatca aaggtgagag gcacatgccg gtaattaaag
    19081 tacgtgaaaa cgagccgttc gacgtagctc tgcgtcgctt caagcgttcc tgcgaaaaag
    19141 caggtgttct ggcggaagtt cgtcgtcgtg agttctatga aaaaccgact accgaacgta
    19201 agcgcgctaa agcttctgca gtgaaacgtc acgcgaagaa actggctcgc gaaaacgcac
    19261 gccgcactcg tctgtactaa ttccccgaga gcgttgctct ccgatcagac cgagttgtag
    19321 ttgtaaggcc gtgcttccga aaggaatgcg cggcttattt tcgtttatga attgctaaaa
    19381 atcggggcct atggctggac gaatcccacg cgtattcatt aatgatctgc tggcacgcac
    19441 tgacatcgtc gatctgatcg atgcccgtgt gaagctgaaa aagcagggca agaatttcca
    19501 cgcgtgttgt ccattccaca acgagaaaac cccgtcattc accgttaacg gtgagaaaca
    19561 gttttaccac tgctttggat gtggcgcgca cggcaacgcg atcgacttcc tgatgaacta
    19621 cgacaagctc gagttcgttg aaacggtcga agagctggca gcaatgcaca atcttgaagt
    19681 gccatttgaa gcaggcagcg gccccagcca gatcgagcgc catcagaggc aaacgcttta
    19741 tcagttgatg gacggtctga atacgtttta ccaacaatct ttacaacaac ctgttgccac
    19801 gtctgcgcgc cagtatctgg aaaaacgcgg attaagccac gaggttatcg ctcgctttgc
    19861 gattggtttt gcgccccccg gctgggacaa cgtcctgaag cggtttggcg gcaatccaga
    19921 aaatcgccag tcattgattg atgcggggat gttggtcact aacgatcagg gacgcagtta
    19981 cgatcgtttc cgcgagcggg tgatgttccc cattcgcgat aaacgcggtc gggtgattgg
    20041 ttttggcggg cgcgtgctgg gcaacgatac ccccaaatac ctgaactcgc cggaaacaga
    20101 cattttccat aaaggccgcc agctttacgg tctttatgaa gcgcagcagg ataacgctga
    20161 acccaatcgt ctgcttgtgg tcgaaggcta tatggacgtg gtggcgctgg cgcaatacgg
    20221 cattaattac gccgttgcgt cgttaggtac gtcaaccacc gccgatcaca tacaactgtt
    20281 gttccgcgcg accaacaatg tcatttgctg ttatgacggc gaccgtgcag gccgcgatgc
    20341 cgcatggcga gcgctggaaa cggcgctgcc ttacatgaca gacggccgtc agctacgctt
    20401 tatgtttttg ccggatggcg aagaccctga cacgctggta cgaaaagaag gtaaagaagc
    20461 gtttgaagcg cggatggagc aggcgatgcc gctttccgca tttctgttta acagcctgat
    20521 gccgcaagtt gatctgagta cccctgacgg gcgcgcacgt ttgagtacgc tggcactgcc
    20581 attgatatcg caagtgccgg gcgaaacgct gcgaatatat cttcgtcagg aattaggcaa
    20641 caaattaggc atacttgatg acagccagct tgaacgatta atgccaaaag cggcagagag
    20701 cggcgtttct cgccctgttc cgcagctaaa acgcacgacc atgcgtatac ttatagggtt
    20761 gctggtgcaa aatccagaat tagcgacgtt ggtcccgccg cttgagaatc tggatgaaaa
    20821 taagctccct ggacttggct tattcagaga actggtcaac acttgtctct cccagccagg
    20881 tctgaccacc gggcaacttt tagagcacta tcgtggtaca aataatgctg ccacccttga
    20941 aaaactgtcg atgtgggacg atatagcaga taagaatatt gctgagcaaa ccttcaccga
    21001 ctcactcaac catatgtttg attcgctgct tgaactgcgc caggaagagt taatcgctcg
    21061 tgagcgcacg catggtttaa gcaacgaaga acgcctggag ctctggacat taaaccagga
    21121 gctggcgaaa aagtgattta acggcttaag tgccgaagag cgatcgggaa gcccccgaca
    21181 gccgcactga gaggcagcgg caaatatata agtacgccct cgtaattatc gttggcggta
    21241 aacaaccgtt ggatttcagc gttaacggct gaaggacatc gggtcaatcg cccaacacca
    21301 acctcatgaa ataagtgtgg ataccgtctt atggagcaaa acccgcagtc acagctgaaa
    21361 cttcttgtca cccgtggtaa ggagcaaggc tatctgacct atgccgaggt caatgaccat
    21421 ctgccggaag atatcgtcga ttcagatcag atcgaagaca tcatccaaat gatcaacgac
    21481 atgggcattc aggtgatgga agaagcaccg gatgccgatg atctgatgct ggctgaaaac
    21541 accgcggacg aagatgctgc cgaagccgcc gcgcaggtgc tttccagcgt ggaatctgaa
    21601 atcgggcgca cgactgaccc ggtacgcatg tacatgcgtg aaatgggcac cgttgaactg
    21661 ttgacccgcg aaggcgaaat tgacatcgct aagcgtattg aagacgggat caaccaggtt
    21721 caatgctccg ttgctgaata tccggaagcg atcacctatc tgctggaaca gtacgatcgt
    21781 gttgaagcag aagaagcgcg tctgtccgat ctgatcaccg gctttgttga cccgaacgca
    21841 gaagaagatc tggcacctac cgccactcac gtcggttctg agctttccca ggaagatctg
    21901 gacgatgacg aagatgaaga cgaagaagat ggcgatgacg acagcgccga tgatgacaac
    21961 agcatcgacc cggaactggc tcgcgaaaaa tttgcggaac tacgcgctca gtacgttgta
    22021 acgcgtgaca ccatcaaagc gaaaggtcgc agtcacgctg ccgctcagga agagatcctg
    22081 aaactgtctg aagtattcaa acagttccgc ctggtgccga agcagtttga ctacctggtc
    22141 aacagcatgc gcgtcatgat ggaccgcgtt cgtacgcaag aacgtctgat catgaagctc
    22201 tgcgttgagc agtgcaaaat gccgaagaaa aacttcatta ccctgtttac cggcaacgaa
    22261 accagcgata cctggttcaa cgcggcaatt gcgatgaaca agccgtggtc ggaaaaactg
    22321 cacgatgtct ctgaagaagt gcatcgcgcc ctgcagaaac tgcagcagat tgaagaagaa
    22381 accggcctga ccatcgagca ggttaaagat atcaaccgtc gtatgtccat cggtgaagcg
    22441 aaagcccgcc gtgcgaagaa agagatggtt gaagcgaact tacgtctggt tatttctatc
    22501 gccaagaaat acaccaaccg tggcttgcag ttccttgacc tgattcagga aggcaacatc
    22561 ggtctgatga aagcggttga taaattcgaa taccgccgtg gttacaagtt ctccacctac
    22621 gcaacctggt ggatccgtca ggcgatcacc cgttctatcg cggatcaggc gcgcaccatc
    22681 cgtattccgg tgcatatgat tgagactatc aacaaactca accgtatttc tcgccagatg
    22741 ctgcaagaga tgggccgtga accgacgccg gaagaactgg ctgaacgtat gctgatgccg
    22801 gaagacaaga tccgcaaagt gctgaagatc gccaaagagc caatctccat ggaaacgccg
    22861 atcggtgatg atgaagattc gcatctgggg gatttcatcg aggataccac ccttgagctg
    22921 ccgctggatt ctgcgaccac cgaaagcctg cgtgcggcaa cgcacgacgt gctggctggc
    22981 ctgaccgcgc gtgaagcgaa agttctgcgt atgcgtttcg gtatcgatat gaacaccgac
    23041 cacacgctgg aagaagtggg taaacagttc gacgttaccc gcgaacgtat ccgtcagatc
    23101 gaagcgaagg cgctgcgcaa actgcgtcac ccgagccgtt ctgaagtgtt gcgtagcttc
    23161 ctggacgatt aatcggtagg ccggatcagg cgttacgccg cacccggcac tagtccctct
    23221 gcacaaacgc cacctttt
//
LOCUS       DAZPEP010000053        22786 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZPEP010000053 DAZPEP010000000
VERSION     DAZPEP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.053"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..223)
                     /locus_tag="ACHUT9_002050"
     CDS             complement(<1..223)
                     /locus_tag="ACHUT9_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1348526.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYESYIANYPEAG"
     gene            complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHUT9_002051"
     CDS             complement(233..784)
                     /gene="ybcL"
                     /locus_tag="ACHUT9_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFR1348527.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHUT9_002052"
     CDS             complement(953..1285)
                     /gene="emrE"
                     /locus_tag="ACHUT9_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFR1348528.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1466..1594
                     /locus_tag="ACHUT9_002053"
                     /pseudo
     CDS             1466..1594
                     /locus_tag="ACHUT9_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHUT9_002054"
     CDS             complement(1619..1933)
                     /gene="fliE"
                     /locus_tag="ACHUT9_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFR1348529.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2149..3807
                     /gene="fliF"
                     /locus_tag="ACHUT9_002055"
     CDS             2149..3807
                     /gene="fliF"
                     /locus_tag="ACHUT9_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFR1348530.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3800..4795
                     /gene="fliG"
                     /locus_tag="ACHUT9_002056"
     CDS             3800..4795
                     /gene="fliG"
                     /locus_tag="ACHUT9_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFR1348531.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4788..5474
                     /gene="fliH"
                     /locus_tag="ACHUT9_002057"
     CDS             4788..5474
                     /gene="fliH"
                     /locus_tag="ACHUT9_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFR1348532.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5474..6847
                     /gene="fliI"
                     /locus_tag="ACHUT9_002058"
     CDS             5474..6847
                     /gene="fliI"
                     /locus_tag="ACHUT9_002058"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFR1348533.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHUT9_002059"
     CDS             6866..7309
                     /gene="fliJ"
                     /locus_tag="ACHUT9_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFR1348534.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7306..8433
                     /locus_tag="ACHUT9_002060"
     CDS             7306..8433
                     /locus_tag="ACHUT9_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFR1348535.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8538..9002
                     /gene="fliL"
                     /locus_tag="ACHUT9_002061"
     CDS             8538..9002
                     /gene="fliL"
                     /locus_tag="ACHUT9_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFR1348536.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            9007..10011
                     /gene="fliM"
                     /locus_tag="ACHUT9_002062"
     CDS             9007..10011
                     /gene="fliM"
                     /locus_tag="ACHUT9_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFR1348537.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10008..10421
                     /gene="fliN"
                     /locus_tag="ACHUT9_002063"
     CDS             10008..10421
                     /gene="fliN"
                     /locus_tag="ACHUT9_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFR1348538.1"
                     /translation="MSDINNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10424..10789
                     /gene="fliO"
                     /locus_tag="ACHUT9_002064"
     CDS             10424..10789
                     /gene="fliO"
                     /locus_tag="ACHUT9_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFR1348539.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            10789..11526
                     /gene="fliP"
                     /locus_tag="ACHUT9_002065"
     CDS             10789..11526
                     /gene="fliP"
                     /locus_tag="ACHUT9_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFR1348540.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHUT9_002066"
     CDS             11536..11805
                     /gene="fliQ"
                     /locus_tag="ACHUT9_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFR1348541.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11814..12599
                     /gene="fliR"
                     /locus_tag="ACHUT9_002067"
     CDS             11814..12599
                     /gene="fliR"
                     /locus_tag="ACHUT9_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFR1348542.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHUT9_002068"
     CDS             12884..13507
                     /gene="rcsA"
                     /locus_tag="ACHUT9_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFR1348543.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHUT9_002069"
     CDS             complement(13551..13739)
                     /gene="dsrB"
                     /locus_tag="ACHUT9_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFR1348544.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            13902..14129
                     /gene="yodD"
                     /locus_tag="ACHUT9_002070"
     CDS             13902..14129
                     /gene="yodD"
                     /locus_tag="ACHUT9_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFR1348545.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14427..15242
                     /locus_tag="ACHUT9_002071"
     CDS             14427..15242
                     /locus_tag="ACHUT9_002071"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFR1348546.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHUT9_002072"
     CDS             complement(15239..16933)
                     /gene="dgcQ"
                     /locus_tag="ACHUT9_002072"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFR1348547.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(17104..17286)
                     /locus_tag="ACHUT9_002073"
     CDS             complement(17104..17286)
                     /locus_tag="ACHUT9_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFR1348548.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17365..18282)
                     /locus_tag="ACHUT9_002074"
     CDS             complement(17365..18282)
                     /locus_tag="ACHUT9_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFR1348549.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18455..19376
                     /gene="yedA"
                     /locus_tag="ACHUT9_002075"
                     /pseudo
     CDS             18455..19376
                     /gene="yedA"
                     /locus_tag="ACHUT9_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHUT9_002076"
     CDS             complement(19365..19835)
                     /gene="vsr"
                     /locus_tag="ACHUT9_002076"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFR1348550.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHUT9_002077"
     CDS             complement(19816..21234)
                     /gene="dcm"
                     /locus_tag="ACHUT9_002077"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFR1348551.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21301..21957)
                     /locus_tag="ACHUT9_002078"
     CDS             complement(21301..21957)
                     /locus_tag="ACHUT9_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFR1348552.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHUT9_002079"
     CDS             complement(21997..22278)
                     /gene="drpB"
                     /locus_tag="ACHUT9_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFR1348553.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5606 a         5962 c         5730 g         5488 t
ORIGIN      
        1 gacctgcttc tgggtaattc gcaatataag attcatatcc acagggaaat aatattatct
       61 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
      121 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
      181 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
      241 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
      301 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
      361 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
      421 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
      481 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
      541 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
      601 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
      661 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
      721 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
      781 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
      841 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
      901 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
      961 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     1021 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     1081 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     1141 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     1201 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     1261 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     1321 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     1381 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     1441 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     1501 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     1561 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     1621 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     1681 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     1741 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     1801 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     1861 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     1921 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     1981 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     2041 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     2101 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     2161 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     2221 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     2281 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     2341 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     2401 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     2461 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     2521 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     2581 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     2641 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     2701 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     2761 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     2821 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     2881 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     2941 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     3001 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     3061 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     3121 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     3181 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     3241 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     3301 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     3361 gaagatctga cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     3421 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     3481 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     3541 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     3601 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     3661 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     3721 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     3781 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     3841 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     3901 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     3961 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     4021 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     4081 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     4141 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     4201 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     4261 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     4321 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     4381 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     4441 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     4501 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     4561 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     4621 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     4681 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     4741 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     4801 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     4861 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     4921 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     4981 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     5041 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     5101 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     5161 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     5221 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     5281 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     5341 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     5401 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     5461 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     5521 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     5581 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     5641 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     5701 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     5761 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     5821 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     5881 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     5941 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     6001 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     6061 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     6121 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     6181 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     6241 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     6301 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     6361 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     6421 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     6481 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     6541 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     6601 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     6661 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     6721 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     6781 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     6841 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     6901 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     6961 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     7021 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     7081 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     7141 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     7201 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     7261 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     7321 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     7381 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     7441 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     7501 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     7561 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     7621 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     7681 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     7741 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     7801 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     7861 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     7921 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     7981 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     8041 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     8101 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     8161 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     8221 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     8281 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     8341 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     8401 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     8461 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     8521 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     8581 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     8641 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     8701 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     8761 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     8821 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     8881 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
     8941 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
     9001 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
     9061 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
     9121 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
     9181 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
     9241 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
     9301 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
     9361 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
     9421 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
     9481 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
     9541 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
     9601 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
     9661 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
     9721 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
     9781 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
     9841 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
     9901 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
     9961 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacataa
    10021 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    10081 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    10141 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    10201 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    10261 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    10321 taatcgccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    10381 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    10441 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    10501 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    10561 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    10621 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    10681 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    10741 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    10801 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    10861 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    10921 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    10981 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    11041 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    11101 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    11161 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    11221 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    11281 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    11341 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    11401 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    11461 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    11521 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    11581 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    11641 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    11701 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    11761 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    11821 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    11881 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    11941 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    12001 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    12061 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    12121 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    12181 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    12241 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    12301 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    12361 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    12421 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    12481 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    12541 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    12601 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    12661 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    12721 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    12781 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    12841 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    12901 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    12961 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    13021 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    13081 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    13141 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aatttattga taagttctaa
    13201 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    13261 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    13321 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    13381 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    13441 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    13501 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    13561 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    13621 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    13681 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    13741 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    13801 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    13861 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    13921 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    13981 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    14041 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    14101 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    14161 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    14221 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    14281 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    14341 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    14401 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    14461 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    14521 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    14581 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    14641 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    14701 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    14761 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    14821 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    14881 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    14941 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    15001 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    15061 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    15121 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    15181 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    15241 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    15301 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    15361 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    15421 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    15481 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    15541 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    15601 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    15661 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    15721 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    15781 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    15841 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    15901 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    15961 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    16021 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    16081 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    16141 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    16201 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgttcc ggttgcgaag
    16261 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    16321 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    16381 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    16441 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    16501 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    16561 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    16621 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    16681 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    16741 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    16801 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    16861 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    16921 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    16981 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    17041 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    17101 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    17161 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    17221 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    17281 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    17341 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    17401 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    17461 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    17521 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    17581 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    17641 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    17701 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    17761 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    17821 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    17881 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    17941 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    18001 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    18061 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    18121 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    18181 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    18241 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    18301 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    18361 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    18421 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    18481 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    18541 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    18601 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    18661 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    18721 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    18781 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    18841 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    18901 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    18961 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    19021 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    19081 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    19141 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    19201 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    19261 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    19321 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    19381 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    19441 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    19501 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    19561 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    19621 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    19681 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    19741 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    19801 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    19861 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    19921 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    19981 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    20041 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    20101 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    20161 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    20221 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    20281 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    20341 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    20401 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    20461 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    20521 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    20581 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    20641 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    20701 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    20761 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    20821 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    20881 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    20941 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    21001 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    21061 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    21121 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    21181 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    21241 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    21301 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    21361 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    21421 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    21481 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    21541 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    21601 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    21661 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    21721 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    21781 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    21841 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    21901 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    21961 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    22021 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    22081 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    22141 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    22201 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    22261 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    22321 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    22381 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    22441 aacagcaata aagatttatt tatatccttg gtaatagttt tattaattca cccaaacaat
    22501 atattagcac tgaatacaat tatacctttt ttgatttctg tgtcatgata accccgttat
    22561 tagcctttta tcgtcttgtt tatatttttt gggccggcat gatgccggct ttttttatac
    22621 cttcattaat gtgcgcgtga tcacgcctgc cgtttggcgc aacaatcatt gatactccct
    22681 atctttccat tcaactcata acatattgat atacatttat aattttcatg gatgtttata
    22741 tttagaattc ataattctga attatattaa atagattaga aacatt
//
LOCUS       DAZPEP010000054        22738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZPEP010000054 DAZPEP010000000
VERSION     DAZPEP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22738
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.054"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..457)
                     /gene="folK"
                     /locus_tag="ACHUT9_002080"
     CDS             complement(<1..457)
                     /gene="folK"
                     /locus_tag="ACHUT9_002080"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFR1348554.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRA"
     gene            complement(454..1851)
                     /gene="pcnB"
                     /locus_tag="ACHUT9_002081"
     CDS             complement(454..1851)
                     /gene="pcnB"
                     /locus_tag="ACHUT9_002081"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFR1348555.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1911..2807)
                     /gene="gluQRS"
                     /locus_tag="ACHUT9_002082"
     CDS             complement(1911..2807)
                     /gene="gluQRS"
                     /locus_tag="ACHUT9_002082"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFR1348556.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2874..3329)
                     /gene="dksA"
                     /locus_tag="ACHUT9_002083"
     CDS             complement(2874..3329)
                     /gene="dksA"
                     /locus_tag="ACHUT9_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFR1348557.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3507..4211)
                     /gene="sfsA"
                     /locus_tag="ACHUT9_002084"
     CDS             complement(3507..4211)
                     /gene="sfsA"
                     /locus_tag="ACHUT9_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFR1348558.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4226..4756)
                     /gene="thpR"
                     /locus_tag="ACHUT9_002085"
     CDS             complement(4226..4756)
                     /gene="thpR"
                     /locus_tag="ACHUT9_002085"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFR1348559.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4830..7259
                     /gene="hrpB"
                     /locus_tag="ACHUT9_002086"
     CDS             4830..7259
                     /gene="hrpB"
                     /locus_tag="ACHUT9_002086"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFR1348560.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQHLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7455..9989
                     /gene="mrcB"
                     /locus_tag="ACHUT9_002087"
     CDS             7455..9989
                     /gene="mrcB"
                     /locus_tag="ACHUT9_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFR1348561.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10209..12398
                     /gene="fhuA"
                     /locus_tag="ACHUT9_002088"
     CDS             10209..12398
                     /gene="fhuA"
                     /locus_tag="ACHUT9_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFR1348562.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12449..13246
                     /gene="fhuC"
                     /locus_tag="ACHUT9_002089"
     CDS             12449..13246
                     /gene="fhuC"
                     /locus_tag="ACHUT9_002089"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFR1348563.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13246..14136
                     /gene="fhuD"
                     /locus_tag="ACHUT9_002090"
     CDS             13246..14136
                     /gene="fhuD"
                     /locus_tag="ACHUT9_002090"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFR1348564.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14133..16115
                     /gene="fhuB"
                     /locus_tag="ACHUT9_002091"
     CDS             14133..16115
                     /gene="fhuB"
                     /locus_tag="ACHUT9_002091"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFR1348565.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16273..17553)
                     /gene="hemL"
                     /locus_tag="ACHUT9_002092"
     CDS             complement(16273..17553)
                     /gene="hemL"
                     /locus_tag="ACHUT9_002092"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFR1348566.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17719..17766
                     /gene="yadX"
                     /locus_tag="ACHUT9_002093"
     CDS             17719..17766
                     /gene="yadX"
                     /locus_tag="ACHUT9_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFR1348567.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17778..19199
                     /gene="clcA"
                     /locus_tag="ACHUT9_002094"
     CDS             17778..19199
                     /gene="clcA"
                     /locus_tag="ACHUT9_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFR1348568.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19223..19288
                     /gene="yadW"
                     /locus_tag="ACHUT9_002095"
     CDS             19223..19288
                     /gene="yadW"
                     /locus_tag="ACHUT9_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFR1348569.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19281..19625
                     /gene="erpA"
                     /locus_tag="ACHUT9_002096"
     CDS             19281..19625
                     /gene="erpA"
                     /locus_tag="ACHUT9_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFR1348570.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19672..20295)
                     /locus_tag="ACHUT9_002097"
     CDS             complement(19672..20295)
                     /locus_tag="ACHUT9_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFR1348571.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEVGPLIAVCMVVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20333..21133)
                     /gene="btuF"
                     /locus_tag="ACHUT9_002098"
     CDS             complement(20333..21133)
                     /gene="btuF"
                     /locus_tag="ACHUT9_002098"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFR1348572.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21126..21824)
                     /gene="mtnN"
                     /locus_tag="ACHUT9_002099"
     CDS             complement(21126..21824)
                     /gene="mtnN"
                     /locus_tag="ACHUT9_002099"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFR1348573.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21908..>22738
                     /gene="dgt"
                     /locus_tag="ACHUT9_002100"
     CDS             21908..>22738
                     /gene="dgt"
                     /locus_tag="ACHUT9_002100"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1348574.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     L"
BASE COUNT         5142 a         5795 c         6360 g         5441 t
ORIGIN      
        1 atgctcttgt atgtaagact tcacgcaacg tctccccatc aggaaacgcc agctccggtg
       61 caatctcaaa cagcggccac agcataaatc cacgattctt catatcgtag tgcggaacgg
      121 tcaggcgttc ggtatttatc acttcattac caaacagcat gatatcgaga tccagcgtgc
      181 gtggtcccca gcgttcagct ttacgaacac gaccctgttg cagttctatg cgctgtgtat
      241 ggttgagtaa ctcttccggg gcgagcgaag tttcaagcgc cacggcggcg ttgaggtaat
      301 cgggttgatc ttgcggcccc agcggtggag tgcggtaaaa cgaagaaacg gcaagaatgt
      361 ggctttcagg gatatcgcct aatgctttca gggcagcatt gacctgctcc agcggagagg
      421 ccagattgct gcctatggca atatacgcca ctgtcatgcg gtaccctcac gacgcggtgc
      481 gcgtttgcgt ggacgacgag tacggcgacg cggtgacggc tcttcatcca actcgttaag
      541 catccctttt tgatctggtg gcgcggaaac ctggaactca ccccaccatt tcaccagacg
      601 ctgcagttca gcgttacgtt caacttcagc tcgcaaggcc aacaggtcat aagccgcacg
      661 gaacttagga tgctccagca gtttccatgc gcgtttaccc tgacgtcggg acatacgcaa
      721 ctgcaactgc cagatatcgc gggttaacgt cgtcaggcgt ttcgggattg ccagtgaacg
      781 gcaggcttcg tccagcacgt cgttcatcgc cagcgcgaaa gcgtcgtgat aggtcaggcc
      841 gctttcctgg gcgatcttct gtgccgtctc cagcagtggg taccagaaca tggcggcaaa
      901 caagaacgcc gggttcacgc gcatatcgtt atggatacgc gtatcggtat tcttcagcac
      961 ctgttcaatg atccgctcca tcgggctgtc gccattttcc gtgaagtagc gggtaatggt
     1021 cgggaacagc ggctggaaca gatgatattc acacaacagc ttataggttt cgtaaccgta
     1081 gcccgcttgt agcagtttaa gcgattcttc aaacaggcgt gccggtggga tatcgttcag
     1141 cagggtagcg aggcgaggga tcggttctgc ggtttccggg ctgatgcgca tacccaattt
     1201 ggcggcaaaa cgtaccgcgc gcagcatccg taccggatct tcacggtagc gcgtttccgg
     1261 gttaccaatc agacggataa cgccgtcctt cagatccttc ataccgccaa cgtaatcacg
     1321 gacggtaaaa tccgctacgc tgtaatagag gctgttgata gtgaaatcgc ggcgctgggc
     1381 gtcttcttcg atggagccga aaatgttgtc gcgcagcaac atgccgtttt gcccgcgttg
     1441 ggaggtcgtg cggtcgctga cgttaccttc gtggtgtcca cggaaggtcg caacttcgat
     1501 aatctccggg ccaaacatta catgagccag acggaaacgg cgacccacca ggcggcagtt
     1561 acggaacagt ttgcgcacct gctcaggcgt ggcgttagtg gttacgtcaa aatctttcgg
     1621 ctttttgcca agtaacaggt cgcgcacgcc gccgccaacc agccaggctt cgtatcccgc
     1681 tttgttgagc ctgtacatta ccttcagggc attttcactg atatctttgc gggaaatagc
     1741 atgctgctca cgcgggatca ccgtcacctg tggacgggcg actgcctgtt cagcctcgct
     1801 ttcctcgcgg cttagcacct tgcggcaaaa attagcgact cgggtaaaaa tagtacacct
     1861 cggtagtgtc aaacatcatt caggacaaaa aaatagcggc taatcatagc tcagcatgac
     1921 gcatttgaga atgttgaatt tacaattgcc gactcgggca cggcggtcag cgtccagttt
     1981 ttgactgctg actgaaggat ttgttcgacg ctgaaatcct gccagtgtgc ttctgcctgc
     2041 tgacccagaa attgaagtgc cgcgattagt accggacgtg gatcgccttt cggcaacgca
     2101 ggcgcatgat tctgcttgga aagtttagcg ccttgtggat taagcgccag cggcagatga
     2161 atgtaatctg gcactttcca gccaaaaagc tggtacagcg agatttgcct tactgttggt
     2221 tcaatcagat cagccccacg cactatttct gtaacgccct ggaaatgatc atcaaccaca
     2281 acagccaggt tgtaggcgaa caacccatca cggcgatgaa tgataaaatc ttcccgtgcc
     2341 agtttttcgt cggcgtgaat aatgccgcgc agctggtcag taaattgcgt gaccggatgc
     2401 tgctggcgga tacgcactgc ggcattgtct ggtccatgat gcaacacccg gcaatgaccg
     2461 tcgtaaatac cgccaatgct ttgaatacgc gcacgcgtac aggtgcagta ataacttagt
     2521 ccttgttcat gtaaccaggc gagtgcttca cgataggcgt cgtgacgttg cgattgccag
     2581 agaacatcgc cgtcccagtg cagaccgtaa tgttccagct ggcgcaggat agtttctgcg
     2641 gcaccgggaa cttcacgagg cgggtcgata tcttctatgc gtaccagcca gcgaccttgc
     2701 cgggcgcgag cctgcaaata gctgccgagc gcggcgatca gagagccaaa atgaagctcg
     2761 ccggaaggag agggggcgaa gcggccaata tactgtgtgt ctgtcatctc tttgaacaaa
     2821 aaataaggcg ggagcatttc ccgcctgtgg taaacgtgat ggaacggctg taattagcca
     2881 gccatctgtt tttcgcgaat ttcagccagc gttttgcagt cgatgcacag atcggctgtc
     2941 gggcgcgctt ccagacggcg aataccaatt tcaacaccgc aggattcgca gtagccgaaa
     3001 tcttcgtctt ccactttttt cagcgtcttc tcgatctttt tgatcagctt acgctcgcga
     3061 tcgcggttac gcagttcgag gctgaactct tcttcctggg ctgcacggtc taccgggtcc
     3121 gggaagttgg ctgcttcatc ctgcatatgt gtaacggtgc gatcgacttc atccctgagt
     3181 tgattacgcc atgcttccag aatacgacgg aagtgcgcca gctgggcttc attcatatac
     3241 tcttcgcccg gcttctcctg atatggttcc accccagcga tggcgagaat actcagggac
     3301 gatgttttac ggttttgccc ttcttgcatg ttgcttctcc ttaacacgca ctatcgatcc
     3361 ccatgttcgg gggaaaaatg aggccgctat aaatagcaga tgcttttccg gatagcaatt
     3421 atctaaacgt aacacttgac aactgtgtga ggaaaagcgt atttgcgcac gcgaccagaa
     3481 tgtaaattaa ccagttactt actttactac aatgtaaccg acagtgattt tttaagagcc
     3541 atgccttcag cagaaatttc cgctttgtaa gccagaattt ctacccccct ctgttgagct
     3601 tctgacaata gttgcgcgta tttctcatcg atgtggcgcg cgggtgaaaa ccgtgtaatg
     3661 gctgaatgca gcacggcgaa aaagataacc gcacgctggc cttcagccgc tacgttcatc
     3721 aactcccgaa ggtgtttctg acctcgttca gtgaccgcat cgggaaaata tccctgttcg
     3781 ttctccgcta acgtaaccga tttcacttca atatagcagt ctggacgcga atccgcctgc
     3841 aacataaagt caatacggct gcgttctgcg ccgtatttta cttcgctttt cagcgagcta
     3901 tagcctgaca gttctgaaat tgattcatta aggatagcct ctttcgtcaa cctgttagcc
     3961 caaagcgtat tgacgcaaat aaatgcgccg ctctggcttt gagttagttc ccaggtgtgt
     4021 gggtatttcc gtttggtgtt gtctgaagtc gaataccaga cggtatcgcc acgcgttgca
     4081 caaccggtca tcgcacccgt attcgggcag tgtagcgtta attcgcgacc atcgggtgtg
     4141 atcacatcgg ctaaaaaacg tttgtaacgc tgaattagcg tcgcgcgctg tagaggggga
     4201 gagaattcca ttgcgacaat ccttgttatt gcgttagcgc ccagcgtttt agcggcgtgt
     4261 agcgtgtgcg tccacgggca aacgaggagg cgtaaagggt gaactccgtc accgcataag
     4321 accagttaaa acctggcggc gggattgtca ccgcctcgct ggcgtcgcgc aataaggtaa
     4381 tatgtggatg aaacggacga ttgctttgaa aacaaccgct gcgggcagcc tgtgaacgga
     4441 gcatattcgc cagctggatt aagccgcgtg gcggctgacg catccctaac cacaccacac
     4501 gcgaacgcag ccattgtccg gcgtcatcaa gcgtgagtgt gaaaccaggt tgacgaatcc
     4561 gtccggctaa aagagaaagc gccttctctt tctcggcgct cacttcgcct aaaaatgcca
     4621 gagtcagatg caaattatcg gcggcgaccg gacgtcccgc ctcaggtggg aagtgtgtgg
     4681 cgcgccagtg gataatctgt tcgcggattt ctgcaggtaa gtcgatagca aagaacagac
     4741 gttgcggttc agacatgtga ggcactcggt tatgaattac cgcgatgcta caatgtggcg
     4801 cgaagaatgt taaccctctg gagcgttttg tgtcgtcgtt gcccgttgct gccgtcttac
     4861 ctgaattact taccgctctc gattgtgcgc cgcaggtatt attaagtgcg ccgaccgggg
     4921 ccgggaaatc aacctggctg ccgctgcaac tgctggcgca tcccggcatt aacgggaaaa
     4981 ttatcctgct ggagccgcgt cgtctggcgg cgcgtaacgt cgcgcaacgg ctggcggagc
     5041 tgcttaacga aaagccaggc gataccgttg gctaccggat gcgtgcgcaa aactgcgtcg
     5101 ggccaaatac ccgtctggaa gtagttaccg aaggcgtgct gacgcggatg atccagcgtg
     5161 acccggagtt gagcggtgtt ggattggtga tccttgatga atttcatgag cgcagcttgc
     5221 aggcggattt ggcgttggcg ctgttactcg atgtgcaaca aggtctgcgt gatgacctta
     5281 aactgctgat tatgtcggct acgctggata acgaccgctt gcagcaaatg ctgccagaag
     5341 cgcccgtcgt catctcagaa gggcgctcgt ttccggttga acgccattat ttaccgctgc
     5401 ccgcgcatca gcgttttgac gaagccgttg cggtagccac cgccgaaatg ctgcgtcagg
     5461 aaagcggatc attactgtta tttttacctg gcgtcggaga aattcagcgt gtgcaggaac
     5521 aactggcttc gcgcatcggc agtgatgtgt tgctctgccc gctgtatggc gcgttgtcgc
     5581 tgaacgatca gcgaaaagcg atcctcccgg caccgcaagg gatgcgcaaa gtggtgctgg
     5641 cgaccaatat tgctgaaacc agtttaacca ttgaaggtat tcgtctggtg gtggattgtg
     5701 cccaggagcg tgtggcgcgt tttgagccgc gcacggggct tacgcgactg attactcaac
     5761 gcgttagcca ggcatccatg acgcagcgtg ccgggcgcgc cgggcgtctg gagccgggta
     5821 tctgcctgca tttaatcgcc aaagaacaag cagaacgcgc tgccgcgcaa agtgaaccgg
     5881 agatcttaca aagcgatctt tccggtttgc tgatggaatt actgcaatgg ggatgcagcg
     5941 atccggcgca gatgagctgg ctggatcaac cgccagtagt gaatctaatg gccgcgaaac
     6001 gtctgttaca aatgctgggg gcgctggacg gtgaacggct tagtgcgcaa gggcaaaaaa
     6061 tggcagcgct gggtaacgat ccgcgtttag cggcaatgct ggttagcgcg aagaacgacg
     6121 acgaagctgc taccgcggca aaaattgccg ccattctcga agagccgcca ctgatgggca
     6181 atagtgacct gggcgtggcg ttttcgcgca atcaaccagc ctggcagcaa cgtagtcagc
     6241 aactgttaaa acgcttaaac gtacgcggcg gtgaggcaga cagttcgctt atcgcgccgc
     6301 tacttgccgg ggcgtttgcc gatcgcattg ctcatcgccg tgggcaagat ggacgctatc
     6361 aactggcaaa cggcatggga gcgatgctcg atgccgacga cgcgctaagc cgccacgaat
     6421 ggttgatcgc accgttatta ttgcagggca gcgcctcgcc ggatgcgcgg attttactgg
     6481 cgctaccggt cgatattgat gaattagtac aacgctgtcc gcagctggtg cagcaatctg
     6541 acaccgtgga atgggatgac gcgcaaggta cgctgaaagc ctggcgtcgg ctacaaatcg
     6601 gtcagttgac ggtgaaagtg cagccgctgg cgaaaccgtc agaagacgag ttgcatcagg
     6661 cgatgcttaa tggcatccgt gataaaggtt taagcgtgct caactggacg gcggaagcgg
     6721 aacagctacg cttgcgtttg ttatgcgccg caaagtggtt gccggaatat gactggccag
     6781 cggttgatga tgaaagttta ttggcagcgc tggaaacgtg gctgctgcca catatgacag
     6841 gcgtacattc actacgcggc ctgaaatcac tggacattta tcaggcacta cgcggattac
     6901 ttgattgggg aatgcagcaa catctggata gtgaattgcc tgcgcattac actgtgccga
     6961 cgggaagccg gatcgccatt cgttatcatg aagataaccc gcccgcactg gcggtgagaa
     7021 tgcaggagat gtttggtgag gccactaatc cgactatcgc ccaggggggc gtgccgctgg
     7081 tgctggagtt gctttcaccc gcccaaaggc cactacaaat cacacgagat ttgagcgcct
     7141 tctggaaagg agcgtaccgt gaggtgcaaa aagagatgaa agggcgttat cccaaacacg
     7201 tctggccgga cgacccggca aacacggcac cgacgcgacg gacgaaaaag tattcgtaag
     7261 gttggttttc tccctctccc tgtgggagag ggccggggtg agggcatcag cgcgcacgtt
     7321 caccctcacc ctaaccctct ccctcaaggg agaggggacc gatcgagcac aaattttgag
     7381 agatatcttc ttctgtcttg taacagaaga acagaaaatc gggcttttgc gcctgaatat
     7441 tgcggagaaa aagcatggcc gggaatgacc gcgagccaat tggacgcaaa gggaaaccga
     7501 cgcgtccggt caaacaaaag gtaagccgtc gtcgttacga agatgacgat gattacgacg
     7561 attatgatga ctatgaggat gaagaaccga tgccgcgcaa aggtaagggc aaaggcaaag
     7621 ggcgtaagcc tcgtggcaaa cgcggctggc tatggctgct gttaaaactg gctatcgttt
     7681 ttgccgtgct gatcgccatt tacggcgttt atctcgatca aaaaattcgt agccgtattg
     7741 atggcaaggt ctggcaactg cccgcggcag tttatggccg aatggtcaac cttgaaccag
     7801 atatgaccat cagcaagaac gaaatggtga agctgctgga ggcaacacag tatcgtcagg
     7861 tgtcgaaaat gacccgtcct ggcgaattta ccgtgcaggc caacagcatt gagatgatcc
     7921 gccgtccgtt tgatttcccg gacagcaaag aagggcaggt gcgtgcgcgt ctgacctttg
     7981 atggcgatca tctggcgacg atcgtcaata tggagaacaa ccgtcagttc ggtttcttcc
     8041 gtcttgatcc gcgcctgatc accatgatct cttcgccaaa cggtgagcag cgtctgtttg
     8101 tgccgcgcag tggtttcccg gacttgctgg tggatacttt gctggcgaca gaagaccgtc
     8161 atttctacga gcatgatggg atcagtctct actccatcgg acgtgcggtg ctggcaaacc
     8221 tgaccgccgg acgcacggtg cagggagcga gtacgctgac gcaacagctg gtgaaaaacc
     8281 tgttcctctc cagcgagcgt tcttactggc gtaaagcgaa cgaagcttac atggcgctga
     8341 tcatggacgc gcgttacagc aaagaccgta ttcttgagct gtatatgaac gaggtgtatc
     8401 tcggtcagag cggcgacaac gaaatccgcg gcttcccgct tgcgagcttg tattactttg
     8461 gtcgcccggt agaagagctc agcctcgacc agcaggcgct gttagtcggt atggtgaaag
     8521 gggcgtccat ctacaacccg tggcgtaacc cgaaactggc gctggagcga cgtaatctgg
     8581 tgctgcgttt gctacaacaa cagcagatta tcgatggtga actgtatgaa atgctcagcg
     8641 cgcgtcctct gggcgtacag ccgcgcggtg gggtaatctc tccacagcca gcctttatgc
     8701 aactggtgcg tcaggagctg caggcgaaat taggcgataa ggtaaaagat ctctccggcg
     8761 tgaagatctt cactaccttt gactcggtgg cccaggacgc ggcagaaaaa gccgccgtgg
     8821 aaggcattcc ggcactgaag aaacagcgta agttgagcga tcttgaaact gcaattgtgg
     8881 tcgtcgaccg ctttagtggt gaagttcgtg cgatggtggg aggttctgag ccgcagtttg
     8941 cgggctacaa ccgtgcgatg caggcgcgtc gttcgattgg ttcccttgca aaaccagcga
     9001 cttatctgac ggctttaagc cagccgaaaa tctatcgcct gaacacgtgg attgccgatg
     9061 cgccaatcgc gctgcgtcag ccgaatggcc aggtctggtc accgcagaac gatgaccgtc
     9121 gttatagcga aagcggcaga gtgatgctgg tggatgcgtt gacccgttcg atgaacgtgc
     9181 cgacggtaaa tctggggatg gcgctggggc tgcctgcagt tacggagacc tggatcaaac
     9241 tgggcgtgcc gaaagatcag ctgcatccgg ttccggcgat gctgctgggg gcgttgaact
     9301 taacgccaat cgaagtggcg caggcattcc agaccatcgc cagcggcggt aaccgtgcac
     9361 cgctttctgc actgcgttcg gtaatcgcgg aagatggcaa ggtgctgtat cagagcttcc
     9421 cgcaggcaga acgcgctgtt ccggcgcagg cggcgtatct gacactatgg accatgcagc
     9481 aggtggtaca acgtggtacg ggtcgtcaac ttggggcgaa atacccgaac ctgcatctgg
     9541 cagggaaaac agggactacc aacaataacg tagatacctg gtttgcgggc attgacggca
     9601 gcacggtgac catcacctgg gtaggccgtg ataacaacca gccgaccaaa ctgtatggtg
     9661 ccagcggggc aatgtctatt tatcagcgtt atctggctaa ccagacgcca acgccgctga
     9721 atcttgttcc gccagaagat attgcagata tgggcgtgga ctacgacggc aactttgttt
     9781 gcagcggtgg catgcgtgtc ttgccggtct ggaccagcga tccgcaatcg ctgtgccagc
     9841 agagcgagat gcagcagcag ccgtcaggca atccgtttga tcagtcttct cagccgcagc
     9901 aacagccgca acagcaacct gctcagcaag agcagaaaga cagcgacggt gtagccggtt
     9961 ggatcaagga tatgtttggt agtaattaac atctaagcgt gaaataccgg atggcgaatt
    10021 gccatccggt aaaataacat cccacctaaa gtattaaccc tttcttttca tctggttgtt
    10081 tattaaccct tcaggaacgc tcagattgcg taccgcttgc gaacccgcca gcgtttcgaa
    10141 tattatctta tctttataat aatcattctc gtttacgtta tcattcactt tacatcagag
    10201 atataccaat ggcgcgttcc aaaactgctc agccaaaaca ctcactgcgt aaaatcgcag
    10261 ttgtagtagc cacagcggtt agcggcatgt ctgtttatgc acaggcagcg gttgaaccga
    10321 aagaagacac tatcaccgtt accgctgcac ctgcgccgca agaaagcgca tgggggcctg
    10381 ctgcaactat tgcggcgaag cactctgcta ctgcgactaa aacggataca ccaattgaaa
    10441 aaacgccgca gtctatttcg gttgtgacaa atgaagagat gcagatgcgt caatttcagt
    10501 ctgtaaaaga agcattaggt tatacacctg gcgttacggt tagcagtcgt ggtgcttcta
    10561 atacatatga ttttgttatc attcgtggtt tctcatctgt tggtctgaat caaaataatt
    10621 accttgatgg actaaaactt cagggcaact tttataacga tgctgtgatt gatccttaca
    10681 tgcttgagag ggttgaactg atgcgtgggc cgacgtctgt tctctatgga aaaagtaatc
    10741 ctggtgggat tatttctatg gtgagtaagc gtccgaccac tgaacccctg aaagaaattc
    10801 agtttaaaat ggggacggat aatctgtttc agaccggatt cgatttcagt gatgcactgg
    10861 atgataacgg tgagttctct taccgtttga cgggcctcgc tcgctcaaca aacgaacagc
    10921 agaaaaactc tgaatctcag cgttatacca ttgctccatc attctcatgg cgtccagacg
    10981 acaaaaccaa ttttactttc ctgtcctatt tccagaatga accagaaacg ggttattacg
    11041 gttggttgcc gaaagagggg accgttgagc cattgcctaa tggtaagcgt ctaccgactg
    11101 acttcaatga aggtgcatcg aataatacat actcccgtaa ccagaaaatg gtgggatata
    11161 gttttgaaca tggttttaat gacaccttca ccgtgcgtca gaatctgcgt ttcagtgaaa
    11221 tgaaaacctc acagaaaagt gtttatggca cagggattgc tggtgatggt cataccctaa
    11281 accgcgggac agtggtggat aatgagcgtc tacaaaactt tagcgttgat acccaacttg
    11341 aaagtaagtt tgctacaggt gacgtcgagc atactttgct gacaggggta gacttcatgc
    11401 ggatgcgtaa tgatatcaat gccagctttg gatccgcacc atccatcgat ctttataaca
    11461 aatatcaccc tgaatacttt gcatttggta gcgcagagcc ataccagatg aatgaaagca
    11521 aacaaacagg tatttatgtt caggatcagg cggaatggaa taaatgggta ttcactctgg
    11581 ggggacgcta cgattggtct aagcaagcga ccactgttcg tgaaaactct tatacgccga
    11641 ctgaaggtta tattgagcgc aatgatcatc agttcacctg gcgcggtggt gtaaattact
    11701 tattcgataa tggtatttca ccttacttta gctatagcca gtcctttgaa ccgagtgctt
    11761 tcgatctgtg gagcaacccg cgcatttcct ataagccatc gaaaggtgaa cagtatgaag
    11821 ctggcgtaaa atatgttccg aatgatatgc cggtcgttgt tacgggcgca gtctatcaat
    11881 tgacgaaaac aaataacctg acagcagacc caacaaaccc attagcgcaa gtcccagcag
    11941 gtgagattcg cgctcgtggt gtggaacttg aagcaaaagc ggcgttaaat gccaatatta
    12001 acttgacggc ttcttatacc tacaccgatg cggaatacac caaagacacc aatctcaaag
    12061 gtaaaactcc agaacaagta ccggagcata tggcatctct ctggggggat tataccttca
    12121 atgaagggcc gctttctggt ttaacattgg gaacaggtgg tcgttttatt ggttccagct
    12181 atggtgatcc ggcaaacact tttaaagtgg gtagcgcagc tgtaatggat gctgttgtaa
    12241 aatatgatct ggcacgcttt ggtatggcgg gatccagcct tgctgtgaac gtcaacaatt
    12301 tgctcgatcg tgagtatgtt gccagttgct tccagactta tggctgcttc tggggcgcag
    12361 aacgtcaggt cgttgcaacc gcaaccttcc gtttctaatt tctcttttgg ggcacggatt
    12421 tccgtgccca tttcacaagt tggctgttat gcaggaatac acgaatcatt ccgataccac
    12481 ttttgcactg cgtaatatct cctttcgtgt gcccgggcgc acgcttttgc atccgctgtc
    12541 gttaaccttt cctgccggga aagtgaccgg tctgattggt cacaacggtt ctggtaaatc
    12601 cactctgctc aaaatgcttg gtcgtcatca gcctccgtca gaaggggaga ttcttcttga
    12661 tgcccagccg ctggaaagct ggagcagcaa agcgtttgcc cgcaaagtgg cttatttgcc
    12721 gcagcagctt cctccggcag aagggatgac cgtgcgtgaa ctggtggcga ttggtcgtta
    12781 cccgtggcat ggcgcgctgg ggcgctttgg tgcggcagat cgcgaaaagg tcgaggaagc
    12841 tatctcgctg gttggcttaa aaccgctggc gcatcggctg gtcgatagtc tctctggcgg
    12901 cgaacgtcag cgggcgtgga tcgccatgct ggtggcgcag gatagccgtt gtctgttgct
    12961 cgacgaaccg acttcggcgc tggatatcgc ccaccaggtt gatgtgctgg cgctggtgca
    13021 ccgtttaagt caggaacgtg gcctgacggt cattgccgtg ttgcacgata tcaatatggc
    13081 ggcacgctac tgtgattatc tggtcgccct gcgcagtggt gaaatgattg ctcagggaac
    13141 gcctgcggaa attatgcgcg gcgaaaccct cgaaatgatt tatggcatcc cgatgggtat
    13201 tttgccgcat ccggcgggtg ctgcacctgt gagttttgtt tattgatgag cggcttacct
    13261 cttatttcgc gccgtcgact gttaacggcg atggcgcttt ctccgttgtt atggcagatg
    13321 aataccgccc acgcggcggc tattgacccc aatcgtattg tggcgctgga gtggttgccg
    13381 gtggaattac tgctggcgct cggcatcgtg ccttacggcg tggcggatac catcaactat
    13441 cgcctgtggg tcagcgaacc accattgccg gactcagtga ttgacgtcgg tttgcgtaca
    13501 gaacctaacc ttgaactgct gaccgaaatg aaaccgtcgt ttatggtctg gtcggcagga
    13561 tatggccctt catcagaaat gctggcacgt attgcgccgg gtcgcggatt taacttcagt
    13621 gacggcaaac agccgttggc gatggcgcgt aaatcgctga cggaaatggc agatttactt
    13681 aacctgcaaa gcgcagcgga aacgcattta gcgcaatacg aagactttat ccgcagcatg
    13741 aaaccccgct ttgtgaagcg tggcgcacgc ccgttactgc tgacgacgct tatcgatccg
    13801 cgccatatgc tggtcttcgg tccaaacagc ttgttccagg aaattctcga tgagtacggc
    13861 atcccaaatg cctggcaagg ggaaaccaac ttctggggca gtaccgccgt cagtatcgat
    13921 cgtctggcag cgtataaaga cgttgatgtg ctctgttttg atcacgacaa cagcaaagac
    13981 atggatgcgc taatggcaac cccgctgtgg caggccatgc cgtttgtccg cgccggacgc
    14041 tttcagcgcg tacctgcagt ctggttttat ggtgcgacac tctcggcaat gcactttgtg
    14101 cgcgttctgg ataacgccat cggaggtaaa gcgtgagtaa acgaattgcg ctttttccgg
    14161 cgttattgct ggcgctgtta gtgattgtcg ctacggcgct cacctggatg aacttctcgc
    14221 aggcgctgcc gcgtagccag tgggcgcagg ctgcctggtc gccagacatc gatgtcatcg
    14281 agcagatgat ttttcactac agcttgttgc cgcgtctggc gatttcgctg ctggtgggcg
    14341 cgggcctggg gctggtgggc gtgctgtttc agcaagtgct gcgtaacccg ctggcggagc
    14401 cgacgacgct tggcgttgct acaggcgcgc aactgggaat taccgtcacc acactttggg
    14461 cgatcccagg tgctatggcg agccagtttg ccgcactggt gggcgcttgt gtcgttggct
    14521 taatcgtctt tggcgtcgcg tgggggaaac ggctttcgcc ggttacgctt attctcgcgg
    14581 ggctggtggt gagcctttat tgcggggcaa ttaatcagct actggttatc ttccatcacg
    14641 accagctgca aagcatgttc ctgtggagca ccggaacgct gacgcaaacc gactggggcg
    14701 gcgttgagcg tttatggccg cagttgctgg gcggcgtgat gttgacgtta ttgctacttc
    14761 gcccgttaac cctgatgggg cttgatgatg gcgtggcgcg caatctcggg ctggccttgt
    14821 cgctcgcgcg tctggcggcg ctgtcgctgg cgatagtcat cagtgcgctg ctggtgaatg
    14881 cggtggggat tatcggcttt atcggtttgt tcgcgccgct gctggcgaaa atgctggggg
    14941 cgcggcgttt gttgccacgg ttgatgctgg cgtcgctgat tggggcgctg atcctctggc
    15001 tttccgatca aatcatcctc tggctgactc gcgtgtggat ggaagtgtcc accggttcgg
    15061 tcactgcgtt gatcggtgcg ccgctgctac tgtggctgtt gccgcgttta cgcagcatta
    15121 gcgcgccgga tatgaaggtc aacgatcgtg tcgcggctga acgccaacat gtgctggcgt
    15181 ttgccctcgc gggcggcgtg ctgctgttga tggctgtggt ggtggcgctg tcgtttggtc
    15241 gtgatgcgca cggctggacg tgggcgagcg gggcgttgct cgaggatttg atgccctggc
    15301 gctggccgcg aattatggcg gcgctgtttg cgggcgtcat gctggcggtg gcgggctgta
    15361 ttattcagcg gctgaccgga aacccgatgg caagcccgga agtgctgggg attagctctg
    15421 gcgcggcgtt tggcgtggtg ttaatgctgt ttctggtgcc gggtaatgcc tttggctggc
    15481 tgttacccgc gggcagtatc ggggcggcgg tgacgctgtt gatcattatg atcgccgccg
    15541 gccgcggtgg attttcccca caccgtatgt tactggcggg gatggcgtta agtaccgcat
    15601 tcaccatgct tttgatgatg ttgcaggcga gcggtgaccc gcgaatggcg caagtgctga
    15661 cctggatttc cggttcgacc tacaacgcga ccgatgcgca ggtctggcgt accggaattg
    15721 tgatggtgat tttgctggcg attaccccgc tgtaccgccg ctggctgacc attttaccgc
    15781 tgggtggtga taccgcacga gccgtaggaa tggcgctgac gccgacgcga attgcgctgc
    15841 tgctgttagc ggcttgcctg acggcgaccg cgacgatgac tattggaccg ttgagttttg
    15901 ttggtttaat ggcaccgcat attgcgcgga tgatgggctt tcgacggacg atgccacaca
    15961 tcgtaatttc ggcgctggtg ggtggtttac tgctggtatt cgctgactgg tgtgggcgga
    16021 tggtgctgtt tccattccag atcccggcgg ggctgctgtc gacctttatc ggcgcgccat
    16081 attttatcta tttgctgaga aagcagagcc gttaattttt ccatataacg gccctgtacg
    16141 cctggacgga taaagcgttt acgcgacaac ggatgcccga tgcgacgctg gcgcgtctta
    16201 tcgggcctac aaacggcccc gaatcgtagg tcggataagg cgttcacgcc gcatccgaca
    16261 aaccatgctg gatcacaact tcgcaaacac ccgacgtgca gcatcgatgg tgttattgat
    16321 atcttccatg ctgtgcgcca cggacataaa gcccgcttca aacgctgacg gtgccaggta
    16381 aacaccttcg tccagcatca tatggaagaa acgcttaaag cgttccacgt cacaggccat
    16441 cacatcctga tagcacgtca cggactcggc gtcggtaaag aaaataccga acatgccgcc
    16501 aacgtggtta acgaccagcg gaattccggc ttcttctgcc gcttccagca gaccttctgc
    16561 cagacgtgtt gtcagctcat ccaacgtttc gtgaacgccc ggttgcgcga cttcattcag
    16621 acaggcgaaa ccggctgcca tcgcaattgg gttaccggaa agcgtacccg cctgatagac
    16681 cggacccgtc ggggccagcg catccattac atcacgacga ccaccgaatg cgcctaccgg
    16741 cattccaccg ccgatgattt tgcccaggca ggtgagatcc ggttccacac cgtaataatc
    16801 ctgtgcgcca gccagtgcca cgcggaagcc ggtcattact tcatcgatga tcagcaatgc
    16861 gccaaattcg tcgcacagcg cacgcagacc tggcaggaag tctggcagcg gtggaacaca
    16921 gttcatattg cctgccaccg gctcgatgat aatacaggca atctcttgcg ggtattgctc
    16981 aaacgcggcg cgtacagaag ccagatcgtt ataagtacag gttaaggtat gtttggcgaa
    17041 atctgccgga acgcccggcg agtttggctg gcctaacgtg agtgcgccag aaccggcttt
    17101 caccagcagg cagtcagcgt gaccgtggta acacccttca aatttaatga ttttgtcgcg
    17161 accggtaaaa ccacgggcca gacggatggc gctcatcgtc gcctcggtgc cggagttcac
    17221 catgcgcacc atatccatgg tcgggaccaa ttcggtcacc agttgcgcca ttttcacttc
    17281 catttcggtt ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca cggcattgcg
    17341 gattgccgga tggttatggc ccagcaccat cggcccccag gaaccgacat aatcgatata
    17401 ggctttgcca tcaacatcgt acagataagc gccgtccgct ttttcgataa acagtggagt
    17461 gccgcccacg ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga tcagctcgcg
    17521 cgctgcgctg taaagatttt cagacttact catggagggt tcctgattcg tagaaaaagt
    17581 gaatggctgc tattctatgt tattcataac aagttaaata ctcgtcaaac atcaggctgc
    17641 ttgtactggt gcaatcctga tttcttagag tataaaagtt ttgtgcattt gaaacattac
    17701 gctttgcaaa ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta tttgtatgac
    17761 caataagtga tcattggatg aaaactgata ctccctcttt agaaacaccg caggccgcgc
    17821 gcctgcgacg cagacaactg attcgccaac ttcttgagcg cgataaaacc ccgttagcca
    17881 ttttgtttat ggcggcagtc gtcggcacgc ttgttgggct ggcagcggtt gcttttgaca
    17941 aaggtgtcgc ctggttgcag aaccaacgta tgggggcgct ggtacatact gctgataatt
    18001 atccgcttct gttaaccgtc gcttttctct gttcggcggt gctggcgatg tttggctact
    18061 ttttggtgcg caaatacgcg ccggaagcag gtggttcggg gatcccggaa attgaagggg
    18121 cgctggaaga tcaacgtccc gttcgctggt ggcgtgtatt gccggtgaag ttctttggcg
    18181 ggctggggac actcggcgga ggcatggtgt tggggcgcga agggccaacc gtgcagatcg
    18241 gcggtaacat tggccgtatg gtgcttgata ttttccgcct gaaaggtgac gaagctcgcc
    18301 atacgctgct ggcaaccggt gctgctgcgg ggctggctgc ggcctttaac gcgccgctgg
    18361 cgggtatttt gtttattatc gaagagatgc gtccgcagtt tcgctatacg ttaatttcga
    18421 taaaagcggt atttattggc gtcattatgt cgaccattat gtaccggatt tttaatcatg
    18481 aagttgcgtt gattgacgtc ggtaaacttt ctgatgcgcc gcttaatacg ctgtggcttt
    18541 atctgatcct cggtattatt tttggcattt tcggccctat ttttaataaa tgggtgctgg
    18601 ggatgcagga tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg gtactgatgg
    18661 gcggtgcgat tggcggtctg tgtggattgc tggggtttgt ggcaccagca acgtcgggcg
    18721 gcggttttaa cctgattcct atcgctaccg cggggaattt cagcatggga atgctggtgt
    18781 ttatcttcgt cgctcgggtc attaccacct tactctgctt ctcttccggc gcgccgggcg
    18841 gtatttttgc cccgatgctg gcgctgggta ctgtgctggg aaccgctttc ggaatggttg
    18901 ccgttgagct gtttccgcaa tatcaccttg aggcggggac gtttgctatt gccggaatgg
    18961 gggcattact ggcggcatct attcgcgcgc cgttaacggg gatcattctg gttctggaga
    19021 tgaccgataa ctaccagctc attttgccaa tgattattac cggtcttggc gcaacactat
    19081 tagcgcaatt taccggcggg aaaccgctat actcggcgat tcttgcgcgc acgctggcaa
    19141 aacaggaagc tgagcaactg gcgcgaagca aggccgcatc agccagcgag aatacttgaa
    19201 cgaaatacca gggtattaga taatggcgat tattattggg ttagaatttg cccaattgcc
    19261 gatgtcgttt ggagcaaaat atgagtgatg acgtagcact gccgctggag tttaccgacg
    19321 cagcagccaa caaagttaaa agcctgatcg ctgacgaaga taacccgaat ctgaaattac
    19381 gcgtgtatat cacaggtggc ggttgcagcg gcttccagta tggtttcacc tttgatgatc
    19441 aggtgaacga aggcgatatg accatcgaaa aacagggcgt tggcttggtg gttgatccga
    19501 tgagcctgca atatctggtc ggcggttccg ttgactatac cgaaggactg gaaggttctc
    19561 gtttcatcgt gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct tcctttagca
    19621 tctaatctgt tgttcatgtg ccggataaac tatccggcgc atcttctctc ttcaacgccc
    19681 attctcatcc agcgcaaacg tcggtagctt aagatgccaa cgaatagccg ccagccgaat
    19741 caatagcgtc acgaccatgc ccatcatact ggctgtttcc agtggtacgg aaaatgtgta
    19801 ataagccgta gcgtggacaa taccgccgat aatacaggca gttgcgtaga tttctgtacg
    19861 taaaatcatg gggatctcgc gggccagtac atcacgaatg atcccgccgc caacgccagt
    19921 aatgacgacc atacaaaccg cgattaacgg accgacttcc gcattaaagg ctttattcac
    19981 gccaatgccg acaaacaccg ccagaccaac ggcgtccagc accggcaaca tccattttgg
    20041 taagcgtctt ggctggcgca ccagcacgat ggtcagcatg ctggttacca ttgcaacgac
    20101 cagatcggtg ggatctttca cccaaaatac cgggccgtga tccagcgcca tgtcgcgaat
    20161 tgtcccgccg cctacagcgg taaccacgcc cagtaccaga acaccaaaag ggtccatacg
    20221 caattttccg gctaacaaaa cgccggagat ggcaaatacc gctgtgccga ctatatccag
    20281 ccaatagacg agcattgttc aatccccact gagcacctaa aaaggtcagg cgctaatcta
    20341 cctgtgaaag cgcattacag agctgttgtg cagcgaggat aatacgtggg cttgcacgtt
    20401 caaaccagtc actcgtgaga ggaataacgg gaattttgag ctgttcaccc cagtattgtt
    20461 tgattttagg aatttggtcc ggtccgcctg taatgacaat cgcctgtggc gagcgtgcta
    20521 acacctgttc gcggctaact tgcggccagg gaacccggct gtctttaaag atgttttctc
    20581 cgccacaaac ttcgagtacc tggttttgaa tcgactcttt tccactggta aatggcggat
    20641 taatgccgaa ttgcagaaaa acacgttttt taggtttatc agcatattgc gctttcaatt
    20701 gcgcgtactg atccagcagg gattgcgcgg cttgttcggc cttatccggt tgcggactcc
    20761 agggggccaa ttgacgtaac gcattggcaa tttgttcaat gcttgtcgca tcgacccaca
    20821 tcacttttat tcccagcgaa gccagctggt caacctgccg ctcggcatta cctccacgcc
    20881 aggcaatcac cagatcgggt ttcagcgcga caatgcgttc cagattcatc ccctgccagg
    20941 tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg ctgaccccaa
    21001 ccggcgtgat cccggcggca aaggcaagtt cagtgttggc gggagaaagc gtgatgacgc
    21061 gcggcgcggc gttgagccac agtggcgcaa gaaaagacag ggcgaccagc gccctgaaca
    21121 gtgacttagc catgtgccag tttctgcacc agtgactcaa ccatcaggct ggactgttta
    21181 gcggcaacag ccaggaactc atcgaagcta agatgagact gttgatcggc cacgtcggag
    21241 atggcgcgta cgacaacaaa cgggacgttg aaattgtggc agacatgggc gattgccgtc
    21301 gcttccatct ctacagcaat ggcctgtggg aagttgtggc ggattttcgc cagaccaaca
    21361 gaaccgttga tgaaagcgtc gccgctaaca atcaggccac gtacagcgtt aagattcagt
    21421 tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt cagctttaaa gcctgccgga
    21481 cagcctggta actgaccgta ttcataacca aatgccgtga catccgcgtc gtgataacgc
    21541 gcttcgtccg agacaacgat atcgcccact ttcagcgttg gtgccaggcc accggcagaa
    21601 ccggtgttaa taatcacatc tggcttgcag tgttccaaca gcagagtggc acccagcgcc
    21661 gcagcgactt taccgatgcc cgatttcaga agcgcaacct cggttccatt cagttggccg
    21721 gtatagattt cgcaaccgcc gagactgata gtttgacggt tttcgatttt gtcacgcagc
    21781 agcgtaactt cttcttccat tgcaccaatg atgccgattt tcatagattt actcgcgata
    21841 agcccgattt gaaagcatag tttaccatgc gcttacgggg aagcgtattt ctcacgcggg
    21901 agaggacatg gcacagattg atttccgaaa aaaaataaac tggcatcgtc gttaccgttc
    21961 accgcagggc gttaaaaccg aacatgagat cctgcggatc ttcgagagcg atcgcgggcg
    22021 tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag acccaggttt ttccactgga
    22081 gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc aggtgggacg
    22141 ctacatcgcc aaagaaattt taagccgtct gaaagagctt aaattgctgg aagcatacgg
    22201 cctggatgaa ctgaccggcc cctttgaaag cattgttgag atgtcatgcc tgatgcacga
    22261 tatcggcaat ccgccgtttg gtcattttgg cgaagcagcg ataaatgact ggtttcgcca
    22321 acgtttgcac ccggaagatg ccgaaagcca gcctctgact gacgatcgct gcagcgtggc
    22381 ggcactacgt ttacgggacg gggaagaaca gcttaacgag ctgcggcgca agattcgtca
    22441 ggacttatgt cattttgagg ggaatgcaca aggcattcgt ctggtgcata cattgatgcg
    22501 gatgaatctc acttgggcac aggttggcgg tattttaaaa tatacccgtc cggcgtggtg
    22561 gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa aagccgggtt attatctttc
    22621 tgaagaagcc tatattgccc ggttgcgtaa agaacttaat ttggcgcttt acagtcgttt
    22681 tccattaacg tggattatgg aagctgccga cgacatctcc tattgtgtgg cagacctt
//
LOCUS       DAZPEP010000055        22238 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZPEP010000055 DAZPEP010000000
VERSION     DAZPEP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22238
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.055"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUT9_002101"
     CDS             <1..1463
                     /gene="dgcJ"
                     /locus_tag="ACHUT9_002101"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFR1348575.1"
                     /translation="FIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQKADSSFLYDK
                     YQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQT
                     ASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEP
                     VNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYV
                     AGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQY
                     VSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHFRLYQNVSREN
                     ISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAI
                     TLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIG
                     FSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHUT9_002102"
     CDS             1506..2009
                     /gene="yeaK"
                     /locus_tag="ACHUT9_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFR1348576.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHUT9_002103"
     CDS             complement(2010..2114)
                     /gene="yoaI"
                     /locus_tag="ACHUT9_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFR1348577.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            2284..2730
                     /locus_tag="ACHUT9_002104"
     CDS             2284..2730
                     /locus_tag="ACHUT9_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFR1348578.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2687..3507)
                     /gene="nimR"
                     /locus_tag="ACHUT9_002105"
                     /pseudo
     CDS             complement(2687..3507)
                     /gene="nimR"
                     /locus_tag="ACHUT9_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3604..4785
                     /gene="nimT"
                     /locus_tag="ACHUT9_002106"
     CDS             3604..4785
                     /gene="nimT"
                     /locus_tag="ACHUT9_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFR1348579.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            4840..5187
                     /locus_tag="ACHUT9_002107"
     CDS             4840..5187
                     /locus_tag="ACHUT9_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFR1348580.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(5209..5463)
                     /gene="yoaF"
                     /locus_tag="ACHUT9_002108"
     CDS             complement(5209..5463)
                     /gene="yoaF"
                     /locus_tag="ACHUT9_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFR1348581.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5646..6671
                     /gene="dgcP"
                     /locus_tag="ACHUT9_002109"
     CDS             5646..6671
                     /gene="dgcP"
                     /locus_tag="ACHUT9_002109"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFR1348582.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            6704..6802
                     /locus_tag="ACHUT9_002110"
     CDS             6704..6802
                     /locus_tag="ACHUT9_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFR1348583.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            6805..6879
                     /gene="yoaJ"
                     /locus_tag="ACHUT9_002111"
     CDS             6805..6879
                     /gene="yoaJ"
                     /locus_tag="ACHUT9_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFR1348584.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6938..7186)
                     /locus_tag="ACHUT9_002112"
     CDS             complement(6938..7186)
                     /locus_tag="ACHUT9_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1348585.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(7334..7516)
                     /locus_tag="ACHUT9_002113"
     CDS             complement(7334..7516)
                     /locus_tag="ACHUT9_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFR1348586.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(7520..7879)
                     /gene="yeaR"
                     /locus_tag="ACHUT9_002114"
     CDS             complement(7520..7879)
                     /gene="yeaR"
                     /locus_tag="ACHUT9_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFR1348587.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(8052..8690)
                     /gene="leuE"
                     /locus_tag="ACHUT9_002115"
     CDS             complement(8052..8690)
                     /gene="leuE"
                     /locus_tag="ACHUT9_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFR1348588.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(8817..9740)
                     /gene="dmlR"
                     /locus_tag="ACHUT9_002116"
     CDS             complement(8817..9740)
                     /gene="dmlR"
                     /locus_tag="ACHUT9_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFR1348589.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            9843..10928
                     /gene="dmlA"
                     /locus_tag="ACHUT9_002117"
     CDS             9843..10928
                     /gene="dmlA"
                     /locus_tag="ACHUT9_002117"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFR1348590.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            11179..12789
                     /gene="yeaV"
                     /locus_tag="ACHUT9_002118"
     CDS             11179..12789
                     /gene="yeaV"
                     /locus_tag="ACHUT9_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFR1348591.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            12821..13945
                     /gene="yeaW"
                     /locus_tag="ACHUT9_002119"
     CDS             12821..13945
                     /gene="yeaW"
                     /locus_tag="ACHUT9_002119"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFR1348592.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            14001..14966
                     /gene="yeaX"
                     /locus_tag="ACHUT9_002120"
     CDS             14001..14966
                     /gene="yeaX"
                     /locus_tag="ACHUT9_002120"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFR1348593.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGISITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(15020..16135)
                     /gene="rnd"
                     /locus_tag="ACHUT9_002121"
     CDS             complement(15020..16135)
                     /gene="rnd"
                     /locus_tag="ACHUT9_002121"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFR1348594.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(16217..17902)
                     /gene="fadD"
                     /locus_tag="ACHUT9_002122"
     CDS             complement(16217..17902)
                     /gene="fadD"
                     /locus_tag="ACHUT9_002122"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFR1348595.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(18107..18688)
                     /gene="yeaY"
                     /locus_tag="ACHUT9_002123"
     CDS             complement(18107..18688)
                     /gene="yeaY"
                     /locus_tag="ACHUT9_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFR1348596.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(18728..19423)
                     /gene="tsaB"
                     /locus_tag="ACHUT9_002124"
     CDS             complement(18728..19423)
                     /gene="tsaB"
                     /locus_tag="ACHUT9_002124"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFR1348597.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(19481..21391)
                     /locus_tag="ACHUT9_002125"
     CDS             complement(19481..21391)
                     /locus_tag="ACHUT9_002125"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFR1348598.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            21523..21867
                     /locus_tag="ACHUT9_002126"
     CDS             21523..21867
                     /locus_tag="ACHUT9_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFR1348599.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5449 a         5698 c         5401 g         5690 t
ORIGIN      
        1 attttatcgc cgcaagtgtc attgtgctga catcttcctt ccttattttt gaacttgtcg
       61 ccagcgacag agcaatgagt gcctatctgc gctatatcgt gcagaaagca gactcctcct
      121 ttctttatga taagtatcag aatcagagta ttgccgcgca tgtgatgcgc gctctcgctg
      181 ctgagcagtc ggaagtgtcg ccagaacagc ggcgcgccat ctgcgaggct tttgagtctg
      241 ccaataatac ccatggctta aacctgactg cccataaata cccgggctta cgcggcacac
      301 tacaaaccgc atccactgac tgcgacacaa ttgtggaagc tgcagcacta ttacccgctt
      361 ttgatcaggc agtggaaggc aaccgccacc aggatgatta cggttcaggt cttgggatgg
      421 ccgaagagaa atttcactat tatctcgatc tcaatgaccg ctatgtctat ttttatgagc
      481 cggttaatgt tgaatacttt gcgatgaata actggtcctt cctgcagtca ggaagtattg
      541 gcatcgatcg caaagatatt gaaaaggtat ttaccgggcg taccgtattg tcgagcattt
      601 accaggatca gcgtactaaa cagaacgtga tgagtttgct gacgccggta tatgtcgcag
      661 ggcagctaaa agggattgtg ctgctggata ttaacaaaaa caatttgcgg aatatctttt
      721 acactcatga ccgccctctc ctctggcgtt ttctcaatgt cacgctaacc gataccgatt
      781 cggggcgcga cattatcatc aaccagagcg aagataatct gttccagtat gtcagttacg
      841 tccatgactt accgggcggc attcgtgtct cgttatccat tgatattctt tactttatca
      901 cgtcttcgtg gaaaagcgtt ctgttctgga ttttgacggc gttaattttg ctgaatatgg
      961 tccgaatgca cttccgttta taccaaaatg tgtcgcgaga aaatattagt gatgcgatga
     1021 ctggactgta taatcgcaaa attttaaccc ctgaactgga gcagcggttg cagaaactgg
     1081 tgcaatccgg ttcttcggtg atgtttattg caattgacat ggacaagtta aagcaaataa
     1141 atgacaccct cggtcatcag gagggggatt tagcgattac gttattagct caggcgatta
     1201 aacaatcgat tcgtaaaagt gattatgcca tccgactcgg tggcgatgaa ttctgcatca
     1261 ttcttgtcga ttcgacgccg caaattgcag cacaactgcc tgaacgtatc gaaaaacgtc
     1321 tgcaacatat cgcgccgcag aaagaaatcg gcttctcttc cggtatttac gcgatgaaag
     1381 aaaacgatac gttacatgat gcgtataaag cttccgatga gcgtttatat gtcaataagc
     1441 agaacaaaaa cagccgttca tgataacctt ctgtggttgt ttgcttgtaa tctcaggagc
     1501 gtgaaatgac tgaaatggct aaaggaagcg tgacgcatca gcggttaatc gctttattat
     1561 cacaagaagg tgctaacttc cgcgtcgtta cccatgaagc ggtaggtaaa tgtgaagcgg
     1621 tgtcagaaat tcgtggcacc gctttaggcc agggagctaa ggcactggta tgtaaagtca
     1681 aaggcaatgg cgtaaatcaa catgttctgg cgatcctcgc tgccgatcaa caggccgatc
     1741 tgagccaact tgccagccat ataggcggat tacgcgcttc gttagccagc cctgctgaag
     1801 tcgatgaact cacaggctgt gtcttcggcg cgatcccccc tttcagcttc catccaaaac
     1861 tcaaactggt tgccgaccct ctactcttcg aacgatttga tgaaatcgcc ttcaatgcag
     1921 ggatgctgga taaatccgtt attttgaaaa ccacggatta tttgcgcatt gcgcaaccag
     1981 aactgctcaa tttccgccgc actgcgtaac tagcctgtcc gttcaataag cagaacggac
     2041 aaaaccagca caacagcaat ggcaaaaaac gatgacgtga taatcagtgt ctcgacgaac
     2101 atttgatcgt tcatagcatg cccccgggag atagctgttt tcacgttatt gttagcgtgc
     2161 acaaatggca gtttgatgac aattcgcctg attttttatg caaaaaacgt aaagattttc
     2221 tacttccttc ctgcagcaaa cgtaaagtaa gcaggcttat tattttttgg caaggaaacc
     2281 acgatgtttg atgtcacttt gctgatcctg ctcgggttag ctgcgctggg ctttatcagt
     2341 cataacacca ctgtcgccgt ttcaattctg gtgttgatca ttgtccgcgt cacaccgtta
     2401 agcacctttt tcccgtggat tgaaaaacag gggctaagta tcgggataat cattctgacg
     2461 attggcgtta tggcccccat cgccagcggg acgctaccgc cctcaacctt gattcactcg
     2521 tttctgaact ggaaatcgct ggtggcaatt gcagtaggcg tgattgtctc ctggctgggt
     2581 gggcgcggcg tgaccttaat gggcagccag ccgcaactgg tcgccgggct gctggtaggc
     2641 actgttttag gcgtggcgct gtttcgcggc gtaccggtcg gcccgcttat tgccgcgggt
     2701 ctggtttcgc tgattgtggg gaaacagtag ttaatcccgc aatatatcgg cctggcgttt
     2761 gccccaggcc tttcttaaac atagtaataa aggccgttgt tgaatcatac cccagcgtat
     2821 gagcaacttt ctgtacagta tcgcctttca ctaatccctg aagtgccata atcagctgta
     2881 actgctgacg ccactggcga aaactcaacc ccgtctcttt actatcagtc gcgcaagatt
     2941 gcgttcactc atcgcaaaaa agccagccca ttgccctaat gacccccact cgacgggccc
     3001 tttcgccatc atctccacca tcgtgcggat tttaggatga gaagaaacgg gcaggtgcag
     3061 ctgttgttgt ggctgctgag ggagttcatc aaacaggacc tgaattaatc tgcgggtcat
     3121 cggctcagca cgctgagtcg tcgtcctgtt tgccagggtt aaaatcaatt cccggcataa
     3181 gggcgatatt ttcaatgtgc aacatgttgt cggcattgtc actgcgctgg gttcgataaa
     3241 cagaaaacag agttcagcgt tagctgtcac ttgattgcta tgttccaccc cgcccggtat
     3301 ccaaaccgca tattgcggcg gcaccatcca caaagcattt tccaccgtac aggtaattgc
     3361 accatgtaat gctagtatca gctgtccttt gcggtgctga tgtacaggac tggttaactc
     3421 atccgttccg gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt cgggttcata
     3481 gccattgaga ttcaacctgt gcatcatttt gtccgaattt agcgataatt tgtcatttta
     3541 gcttgattca acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag
     3601 catatgacct gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt
     3661 ctgatgattg ccacaacatt acgcgtcacc tttatcggcg cagcaccgtt actggatacg
     3721 attcgttccg cttactcgct gacgacagcg caaaccggct tattgaccac cctgccatta
     3781 ttggcctttg cgctaatctc acctttggcc accccggtag cgcgacgttt tggtatggaa
     3841 cgtagcctgt ttgccgcgtt acttttgatc tgtgctggta tcgcaattcg ctctctccct
     3901 tcgccttact tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggcaat
     3961 gtcttactgc caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc
     4021 gcatattccc tgacaatggg agctgcagcg gcactgggat cggctatgat agtgccgctg
     4081 gctttgaacg gttttggctg gcaaggcgcg ttgctcatgc tgatgtgttt tcctctgctg
     4141 gctctttttt tatggctgcc acagtggcga agccaacaac atgcaaattt gagtacctcg
     4201 cgcgccttac atactcgggg tatctggcgt tcgccgcttg cctggcaggt aacattgttt
     4261 cttgggatca actcactgat ctattacgtg attattggct ggcttccggc gatcctcatc
     4321 agtcacggct atagcgaagc acaggcgggt tcactgcatg gtttgctgca actagccaca
     4381 gcagctcccg gtttgctgat ccctcttttc ttacatcatg tgaaagatca gcgtggtatt
     4441 gcagcgttcg ttgccttgat gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg
     4501 cacgcgatca cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg
     4561 ttgacgttca ttggtcttcg ggcaagttct gcgcatcagg cggcggcact ctcggggatg
     4621 gcacaatccg tcgggtattt gctggcagcc tgtgggccgc cgctaatggg taaaatacac
     4681 gatgctaacg gtgactggtc cgtaccactg atgggtgttg caatactttc actactgatg
     4741 gcgattttcg gactttgcgc cgggagagac aaagaaattc gctaatatcc ggtgctatag
     4801 tgacgtgaca aatcatgcgt gaaagggaga acaaacacga tgaatattca gtgcaaacgc
     4861 gtttatgatc cggctgaaca gagcgatggt tatcgcgtac tggtcgaccg cctctggccg
     4921 cgcggtatca aaaaaaccga tttagccctt gatgagtggg ataaagaaat cacgccgtca
     4981 acggaactgc gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa
     5041 tatcttgcag aactggcgca acacgagcaa gaaggaaagc ggctggcgga catcgccaaa
     5101 aaacagccgc tgaccctgct ctacgcagca aaaaacacca cgcagaacca tgcgctggtg
     5161 ctggccgact ggctacgtag cttgtgattt tagtacagca tccggcggtt atttttcacc
     5221 agccggatgg tcccgtcgcc acaatgccca ttcatcaatc gtttcaccgc ccggtaattt
     5281 gcaattgttg ctgacccctt gcgctgtcga cactggaatg agcgtcccgc ccttctgctg
     5341 gcaatagacc gacgccggat ttgccatacc aatctgcggc ggtttaggta cttctggctg
     5401 agaagaggtt gaacaaccag ccaggaccag caagcaaggc aaagcaaaac tgataatttt
     5461 catttattga tctcactcat ttatctacgc ttagagtatc gcggtatcgt tttgttttgc
     5521 agcactattt ttattacatt cactcaaaac atattacgtc ttgtttcatc tttgttggtg
     5581 atgttttatc atgcctgcaa agattaaata atcagcattt acccgccgta tcctggagtt
     5641 gtttcgtgtc agatcagatt atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac
     5701 tggaaagtct ggtccgacag cttctggaga tgctggaaat ggtcactgat atggaatcaa
     5761 cctacctgac caaagtggat gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca
     5821 gccagaaaat gcacatcccg gagaatttta ccgtctcgtg ggattactcg ttatgcaaac
     5881 gcgccattga tgaaaactgc tttttcagcg atgaagtccc tgaccgttgg ggcgactgta
     5941 ttgcggcacg caatcttggc atctccacat ttctgagcac accaattcac ttaccggatg
     6001 gatcattcta tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg
     6061 cggaacaggt tttacagtta ttcgccggac tgattgcaca atatattcaa aaagaggcac
     6121 tggttgaaca gttgcgcgaa gccaatgctg cgctgattgc gcaatcgtat accgactcgt
     6181 taaccgggtt gccgaatccg cgggcgattt ttgaaaatct gacgacgctg ttttctctcg
     6241 cccggcatct taaccataag ataatgatcg cgtttatcga tctggataac ttcaaattaa
     6301 tcaatgatcg ttttggtcat aatagtggcg atctgtttct cattcaggtt ggcgagcgcc
     6361 ttaatacgct ccagcaaaat ggcgaagtta ttggtcgtct cggcggtgat gagtttttgg
     6421 ttgtttcact aaacaacgag aatgcggata tttcgtcgct gcgagaacgc attcagcagc
     6481 aaatacgtgg agaatatcac ttaggtgatg ttgatttgta ttatcccggt gccagtcttg
     6541 gcatagtaga agtcgatcct gaaacaaccg atgcagacag tgccctgcat gctgccgata
     6601 ttgcgatgta tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg cattcagcgc
     6661 cacattgctg aggcgtattc acatcctttt gattggtgat aacatgcgaa tcggtattat
     6721 ttttccggtt gtaatcttta ttacagcggt cgtattttta gcatggtttt ttattggcgg
     6781 ctatgctgcc ccgggggcat aaagatgaaa aaaacaacga ttattatgat gggtgtggcg
     6841 attattgtcg tactcggcac tgagctggga tggtggtaac gtcacctcta aaaaatagca
     6901 aaggctgcct gtgtgcagcc tttgtgcaat ttaagcgtta acttttaatc ttcctgtaga
     6961 taaatagcac gacaatcgca ccaataacgg caaccacgaa gctgccaaaa ttgaagccat
     7021 cgactttacc aaagccaaac agcgtgctga tccatccgcc gactacggca ccgactatcc
     7081 ccagcaggat agtcataaag aatccaccac catctttacc tggcatgatc cacttcgcca
     7141 gaataccggc aataagccca aaaataatcc atgacagaat gcccattgtt tcctcactta
     7201 tctgttttgc attagcgggt tagtcgctga taaaaagcat agcacaacat cgggagggca
     7261 agatttgtga cgagcatcac ggaggatttt tttgcgatgg cgcagaaatt gcgccatcaa
     7321 cgatcagtga taattaccaa ccacaaacat catgttcgtt ttccgtgtca taagaacgca
     7381 cggtattcac cagatctttt atcacttcag ccgccacttc tggcaccagc aaagtcatcg
     7441 gcgtctctgt ttcataatcg acagaaacgc cattactgtt attggtgacg gtcacggtat
     7501 acgttgcttt gcccatgatt catttcccgt tatgaatgac tttccgttgt tgcgcacctt
     7561 ccatcaggac ttcaggagcc acgaagaagt caatgttgaa ataagtatcg tcagtcatgg
     7621 cttcaatgtt gtgccacttt tctggaggga acacctcaaa ctgccccgct tcgataagga
     7681 tcacctgatc aggctctgca ctgtgttcat cagcgtagcc gagatatttg accgccccat
     7741 gcataacgga aaggcgtggg taaacccccg ggcgcgttcc tttatcaaga tgacgttcga
     7801 atattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta tgaatataat
     7861 tctgtgggat ttgcagcatc cttttccctc cttcggtgaa tgcgctgaaa acggtttatt
     7921 ccagccgttt cagggtacgc ctgataattt gcattttaaa taccatttat tggttacttt
     7981 ttagcaccat atcagagaaa aatcagggag gattacaagt gggaagcccg cgacaacgcg
     8041 ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac gaacatcaaa
     8101 ccaatcagtg agttgccaac tttagccagt ttctttttgg tacgtatgta ctgcgtgaca
     8161 aaagcaccag aaataatcag gaagctcaaa tagcagaaac tcaccagttc cagcgtcgtc
     8221 gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa ctgtacgaaa
     8281 aacgacacat agaacaaaat ggctttcgga ttagtcaggc tcaaaattaa cgcgcgttta
     8341 aaaatggccc cgtattgggg ctcatcggat ttggcctcgt tatttttacc cttcagggtc
     8401 gcgtagagaa ttttactccc cagatagagc aaataaaacg caccaagata acgtacaatg
     8461 ttgaataata tcggggtggt cttaattaat gtcgccactc cagcccatgc cagaaacatc
     8521 aatactgcat cgccaataaa tacaccgcag gccgcaagat aaccgccttt cataccgcta
     8581 ctgacgctat ttttgagtac aaacagggta tttggccctg gcaccaacac aataaaaatg
     8641 gccccaacca gataggtcca gtaattcaga accccgtatt cagcgaacac attaacctct
     8701 ttaattatct ttcgatcatg cgcgattaaa ggtgaatatg ctaaccaatc tgtagcggct
     8761 tagaaaggag aaaatcaggt tttaacccga tatcaacccg ataattgaat aattaatcag
     8821 gcatgctcca gtgaaaaatt cgggtagtgc tcggcaaaat actggcgtaa aaattctatc
     8881 gttatccgca ctttcgctga cgtcgccagc cttgaaacat aaacggccca gacgttcgct
     8941 ggctggtaat attccggtag cacttgcact aaatgaccac tggcaatgtt ttcgctaaca
     9001 tcccaccagg agcgcagagc aatcccctgc ccatcgagac accactgatg gacaatttct
     9061 ccatgattag acgacagtgg gccggtgact ttaatcgcat ggtggccttc tttattgcgt
     9121 aattgccaga caccaaaagg atgatcgcgc tctttaatga ccaggcacgg caatgcggaa
     9181 agatcggtta aatgtttcgg cgcgccatgt tgcgcaataa attccggcga cgcgcagaga
     9241 atgcggtaat tagtcgccag tttgcgggcg attaaattag gggcgatgtc atcgccaatg
     9301 cgaatatcga gatcaacacc ttcattgacc aaatcgacca accggtcttc cacatcaaaa
     9361 cgtaattcca gttgcggata cgccttagcc agcgctgata acgccggagc caccacctgt
     9421 cgtccaaaac caaagctgct gataatacgc agcatcccct gcggcacctg acgcacgtca
     9481 gaaagttcgt ccatcatctg accgacatcc tgcaaaatcc gctgcgccca ttcataaatc
     9541 cgctctcctt cttcggtaat ggtgacgcgg cgggtggtgc ggtgtaacaa caccacgttt
     9601 agcgtttgct ccagcaaggc gatgcgcttg ctgacgaacg ccggtgaaac gcccagttct
     9661 tccgccacgg cggcaaaacc agcccggcga gccaccagca taaagacgcg caaatcgttc
     9721 agcagcggta aattattcat gattcgtgtt ttatgtttca ccagttacgg ggattaattc
     9781 cttttcagtc aattatagga tggtgatgtt gtcaattttg atggtcagga agtgagaacc
     9841 caatgatgaa aacgatgcgt attgctgcga tcccgggaga tgggattggc aaagaagtcc
     9901 ttcctgaagg gattcgcgta ttacaggctg ccgctgagcg ctggggcttc gacttgagtt
     9961 ttgagcaaat ggagtgggcg agctgcgagt attacagcca tcacggtaaa atgatgccgg
    10021 acgactggca tgagcaactt agccgtttcg acgccatcta ttttggtgcc gtcggctggc
    10081 cggataccgt tccggaccat atttcgttgt ggggttcgct gctgaaattt cgtcgtgaat
    10141 tcgaccagta cgtcaacctg cgcccggttc gtctctttcc tggcgttccc tgcccgctgg
    10201 cgggaaaaca gcctggcgac atcgattttt acgtggtcag ggaaaacacc gaaggcgaat
    10261 attcctcgct cggcggtaga gtgaatgaag gtacagagca tgaagtcgtc attcaggaat
    10321 cggtatttac gcgtcgtggt gtcgatcgca ttttgcgtta tgccttcgaa cttgcgcaaa
    10381 gccgcccacg taagacgcta acttctgcca ctaaatcaaa cggtttagcc atcagcatgc
    10441 cgtactggga tgagcgagtg gaagcaatgg ccgagaatta cccggagatc cgctgggaca
    10501 agcagcatat tgatattctc tgcgcgcgtt ttgtgatgca gccggaacgc ttcgatgtgg
    10561 tggtggcgtc caatttgttt ggcgatatcc tttccgatct tggcccggcc tgcaccggca
    10621 ccattggcat tgccccatcc gccaacctga atccggaacg cactttcccg tcgctcttcg
    10681 agcctgtcca cggttccgcg ccggatatct acgggaaaaa tattgctaac cctatcgcca
    10741 caatttgggc cggtgcgatg atgctcgatt ttctcggcaa tggcgatgag cgtttccagc
    10801 aagcgcataa cggtattctg gcagcgattg aagaagtgat tgctcacggg ccgaaaacac
    10861 cggatatgaa aggcagtgcc accacgccac aggttgccga cgcgatttgc aaaattattt
    10921 tgcgttaagg tcaaaccagt ttatttgaac cgcgtcactg acgcggtttt tttattcgtt
    10981 ctttgcagtt aataacctgc gtcatttcac cttttattgt ttccgtttcg tgttttatgg
    11041 ctttccgtat tcttaattgt ttaatttatg taacatgcaa ataatcaatt tgttaaacac
    11101 aacatacaac tcattaccct gcaaataatt acaactggca aaagccagag acgggctttt
    11161 atgcccagga gatgactcat gatgagcaat gttaagaaaa aagatgtgcc gctgataagc
    11221 atcagcctgg tggccattct tttcatcgca gctgcattaa gccttttccc acaacaatcg
    11281 gccgacgcgg ccaacacaat atacactttt gttacgcgta cgttaggttc cgccgtacag
    11341 gtattggttt tgctggcaat gggactggtg atttatttag ccaccagtaa atacggcaat
    11401 attcgtcttg gcgaaggaaa accggaatac agcacgctct cctggctgtt tatgtttatt
    11461 tgtgccggtt taggttcttc tacgctttat tggggggttg ctgaatgggc ctattattat
    11521 caaacgcctg gattaaatat cgcaccacgt tcacaacagg cactcgaatt tagcgtccct
    11581 tactccttct tccactgggg catcagcacc tgggcaactt atacgctggc ctcattaatc
    11641 atggcttatc actttcatgt gcgtaaaaac aaaggtctga gtctttccgg cattattgcc
    11701 gcgattaccg gcgttcgccc gcaaggccca tggggaaaac tggttgattt gatgtttctg
    11761 atcgccactg tcggcgcact gactatttcc cttgttgtca ccgcagcaac ctttactcgt
    11821 ggactttccg cgctgaccgg tttacccgat aacttcaccg tgcaggcatt tgtgatcctg
    11881 ctttccggcg gcattttttg cctaagctcg tggattggta tcaacaacgg tttgcaacgt
    11941 ctgagcaaaa tggttggctg gggcgcgttc ctgctgccat tactggtgct gattgtcggt
    12001 ccaaccgaat ttattaccaa cagcatcatc aatgccatcg gcctgaccac gcaaaacttc
    12061 ctgcaaatga gcttattcac cgatccgctt ggcgatggtt catttacccg caactggacc
    12121 gttttctact ggctgtggtg gatctcatac acccctggcg tagcaatgtt tgtcacccgc
    12181 gtttcccgcg gtcgtaagat taaagaagtt atctggggac tgatcctcgg cagcaccgtc
    12241 ggttgctggt tcttcttcgg cgtaatggaa agctatgcca ttcatcagtt tatcaatggc
    12301 gtaatcaacg tcccacaggt gctggaaaca ctgggcggcg aaacagctgt acagcaagtt
    12361 ctgatgtcgt tgccagccgg taaattgttc ctcgccgcat acctgggcgt gatgattatt
    12421 ttccttgcct cgcatatgga tgcagtggcc tacaccatgg cggcgaccag tacgcgtaat
    12481 ctccaggaag gtgacgatcc tgaccgtggg ctgcgtcttt tctggtgcgt ggtgatcact
    12541 ctgatcccgc tttccatctt gtttaccggt gcttcgctgg aaacgatgaa aaccaccgtc
    12601 gtgctcacag cccttccctt cctcgtcatt ttactggtga aagtcggcgg atttattcgc
    12661 tggctgaaac aggattatgc cgacattccg gctcatcaag ttgaacatta tctcccgcag
    12721 acaccggttg aagccctgga aaaaacacca gtgctccctg cgggaaccgt attcaaaggc
    12781 gacaactgag cgccatcatc ctaacgataa aggtatccct atgagcaatc tgagccctga
    12841 ctttgtacta cccgaaaatt tttgcgctaa cccgcaagag gcgtggacca ttcctgcccg
    12901 tttttatacc gatcagaacg cgtttgaaca cgaaaaagag aacgtcttcg ccaaaagctg
    12961 gatttgcgtc gctcacagca gcgaactggc gaatgccaat gattatgtga cgcgtgagat
    13021 cattggcgaa agcatcgtgc tggtacgcgg tcgtgataag gttttgcgcg cgttctataa
    13081 cgtgtgtccg caccgtggtc atcagttgtt gagcggtgaa ggaaaagcaa aaaatgtgat
    13141 tacctgccca tatcacgcat gggcattcaa actcgatggc aatctggccc atgcacgtaa
    13201 ctgcgaaaac gtcgccaatt tcgatagcga caaagcgcaa ctggttccgg tgcgtctgga
    13261 agaatatgcc ggattcgtct tcatcaacat ggaccccaac gccaccagcg tagaagatca
    13321 gttacccggt ctgggcgcga aagtgctgga agcctgcccg gaagtccacg atctgaaact
    13381 ggcggcccgc tttaccaccc gcacgcctgc caactggaag aacattgtcg ataactatct
    13441 cgagtgctat cactgtggtc cggcgcatcc aggtttctcc gactccgtac aggttgatcg
    13501 ctactggcac accatgcacg gtaactggac gctgcaatac ggtttcgcca aaccgtccga
    13561 acagtcgttt aaatttgaag agggtacgga tgcggcattc cacggtttct ggctgtggcc
    13621 gtgcacgatg ctgaacgtca ccccgatcaa agggatgatg acggtcattt atgaattccc
    13681 ggtggattct gaaactaccc tgcaaaacta cgatatttac ttcaccaatg aagagttaac
    13741 cgacgagcaa aaatcgctga ttgagtggta tcgcgatgtg ttccgtccgg aagatttacg
    13801 tctggttgaa agcgtacaga aagggctgaa atcgcgtggc tatcgtggtc aggggcgcat
    13861 catggccgac agtagcggta gtggcatttc cgaacatggt atcgcccatt tccataatct
    13921 gctggcgcag gtgtttaagg actaatgaca tcggcggcgg tattttccgc cgctgggctg
    13981 atttttgatg gagtacagca atgtcagact atcaaatgtt tgaagtacag gtgagtcagg
    14041 tggaaccgct taccgaacag gtgaaacgct tcacgctggt ggcaaccgat ggcaaaccgc
    14101 tgcctgcgtt caccggagga agtcacatca ttgtgcagat gagcgatggt gataaccagt
    14161 acagcaatgc gtattcacta ctgagttcgc cgcatgacac ctcttgttat cagattgccg
    14221 ttcggctgga ggaaaactcg cgcggcggtt cccgcttttt gcatcagcag gtaaaagtgg
    14281 gcgatcggtt aacgatttca acgcctaata acctgtttgc gctaattccc tcagccagaa
    14341 agcatctgtt tatcgcgggc ggtattagta tcaccccttt cctgtcgcac atggcagagt
    14401 tgcaacacag cgacgtcgac tggcagctac attactgctc gcgaaatcca gaaagttgcg
    14461 catttcgtga tgagctagtc cagcatccgc aggctgagaa agtccatttg catcattcat
    14521 caaccggaac acgactggaa ttagcgcgat tattggcgga tatcgaacct ggcacacacg
    14581 tttatacctg tggccccgag gcgctaaatg aagcggtaag aagtgaagct gcgcgtctgg
    14641 acatcgccgc cgatacgctg cactttgagc aatttgctat cgaagacaaa accggcgatg
    14701 cctttaccct ggtgcttgcc cgttccggaa aagagtttgt ggtgccggaa gagatgacta
    14761 ttttgcaggt tattgaaaat aataaagccg cgaaagtgga atgtttatgt cgtgaagggg
    14821 tatgcggaac ctgcgaaaca gcaatactgg aaggtgaagc tgaccatcgg gatcagtatt
    14881 ttagcgatga agagcgtgcc agccagcaaa gtatgttgat ctgttgttcg cgtgcgaagg
    14941 gtaaacgcct ggtgttggat ttgtagtttg ccaaagccgg atgtggcgct gagcgcgccc
    15001 agtccggctt cggaagattt tactgcggat attcctgcaa taaattgtgt aatgcttccg
    15061 ccatcagctc accacgccag ccggaaatca gctccggcaa attgttctgc ggtttcagtt
    15121 tccagtgcca gttcagcagt tggttgattt gccgacgcga tgccagcaat tcggcgctga
    15181 tcttatgcgt ttcgctcacg tcagtaatca gcgacttaat cgctttaaac gctttacgat
    15241 aacccggcat gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgtct
    15301 gcgctttttc caccagcgcc agcagcgttt taccgtgaaa gcggatttcg ctaccggata
    15361 aacccaggct gtccagttcg cctaaacttc ccggcatata acgcgcgacc gaccacaaat
    15421 gctcttcacg cacgacaaag ttcaccgcca gatcacgctc acgagccttg cgcagtcgcc
    15481 agtcggctaa cagttgcaga caggccagtt ggcgtgtgcg taattgccag gcattggtga
    15541 tatcacgcca ggcatcttcc ggcgcaacga cttcctgacg acgcatttgc atcaggcggc
    15601 attcatccag cgccgcaggt agccagccgg aggcctccgt ttctaccata agcttggcgg
    15661 tgatcggtaa cagataccag acatccgccg ctgcgtattc acactgacgt tcggtcagcg
    15721 gtctggccag ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg gaatactctt
    15781 ccaccatgga agcgaaaccc catgacatcg ggcgtccgca gaaggcagca aggatttgcg
    15841 tgtcaatcag gggttgtggt aattcgccaa agacattgag gaacacttcc agatcttcac
    15901 tgcctgcatg gagaaatttt gtgatggacg gatcgcgcag gatcgctttc agcggtgacc
    15961 agtcggtgat cccgagagga tcaattagcg ccagatgctc accatcgaaa agttgaatca
    16021 accccagctg cgggtaataa gtgcgcgtac gaacaaattc agtatcctgg gctatcgccg
    16081 gaaagacacg gacggcttca cacaaagaag ccagcgcatc gtccgtggta atcatttggt
    16141 aattcaaatt gtattctctt tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg
    16201 acgactgact taacgctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt
    16261 ctcgtcgcaa aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct
    16321 tcggtacttt gtagcccgtg agctgacggc agcaaaaggt caccagtgac tcttcggtaa
    16381 gcgatggatc ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta
    16441 cgccaacagc cgcgacttcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt
    16501 tgggatagac gttaaaaccg gaaaccagaa tcatgtcttt tttacgatcg acaatgcgca
    16561 ggaacccttc ttcatccatc accgcgatgt cgccggtgtg taaccagcca tttttgatga
    16621 tctcatcggt agcatcggga cgctgccagt aacccagcat cacctgcggt cctttgacac
    16681 aaagctcacc cggttgaccc ggtggtactt cattatcgtc gtcatccacc agtttggctt
    16741 ccgtcgacgg caccggcaaa ccgatgctac cactatgata atcaatatca tatgggttaa
    16801 cgctgaccag cggcgcacac tcggtaagac catagccttc cagcagatac tgtcctgtca
    16861 gtttcaccca acgctctgcc accacttgct gcactggcat cccaccgcct gcggaaagat
    16921 gcagactgga gaaatccagc tgctggaact ctttattgtt cagcaacgca ttgaacaagg
    16981 tgttaacgcc agtgatagcg gtaaacggat atttcgctaa ctcttttacc aaccctggaa
    17041 tatcgcgcgg gttagtgata agcaggttct gcccacccag ttcgataaac agcaggcagt
    17101 taatggtcag ggcaaaaatg tgatacagcg gcagtgccgt tactaccagt tctttacctg
    17161 gatgcagcaa cggcccatag gtggcgttga cctgttccag gttcgccagc atattgcggt
    17221 gagtcagcat cgcgcctttc gccacacctg tggtgccgcc ggtgtattgc aggaaggcta
    17281 aatcttccgg caccagttcg ggtttgacgt actgcatccg gtagccgtta tgcagtgcgc
    17341 tacgaaatga aatggcatct ggcagatggt atttcggcac caaacgcttg atgtatttaa
    17401 caacgaaatt gactaccgtg ccttttgccg tagatagctg atcgcccata cgggtcagaa
    17461 ttacgtgctg aacggcggtt ttatcaacca ctttttccag tgtgtgagca aagttagaca
    17521 cgataacaat cgccgatgcg ccgctatcgt taagctgatg ctcaagctca cgcggggtat
    17581 acaacgggtt aacgtttacg acgatcatcc cggcacgcaa aatgccaaac agcgccaccg
    17641 gatattgcaa taaattaggc atcatcaacg caacgcgatc gcctttcttc agccccaacc
    17701 cttgttgcaa ataagcggca aacgcgcgac tgcgttcttc cagcttgcgg aaggtcatta
    17761 cctcccccat attcacaaac gcaggctgat cggcgtagcg gacgaccgac tgctcaaaca
    17821 tatctatcag agattgataa cggtcagggt tgatctccgt cggaacatcc gcgggataac
    17881 ggttaagcca aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa
    17941 ccccaagtga taaacatgtt gttaacataa cattaactca tcttaccagc ttgataatta
    18001 gacaacgaaa aggttgcgaa gcgcgtcact atttattttt atagttgccg tatgaacgca
    18061 gaaacagcgg accagccgct gtttcttttt ctttaaaaac aacttgttac tcggttacaa
    18121 ttgtttcaac tcttgcaggg ccaggattat accaacccca tccgccgtaa ccatagggcc
    18181 agccgcgtcc accataaaac catggatcaa ttggctgcgg cggcataatc acctgttgag
    18241 tgatatgcca gcgtttgtaa cctgttacct gcataaccat aaatttatat ggtgcattat
    18301 ttactttgcc atccactgtg ccagtgattg gcccgacaac cgtcaccagt tgtccacgaa
    18361 aatcgaccgg atcaagaaaa ccgttcacat cggcataaat gcgcccgcgt gaaggttctc
    18421 ccaaggttgg tcttgctccg ctgtccagcg gtacggtagc aatttccagg cgggttttcc
    18481 cttgctggtt ttgtaccaca accactttgc cgccaaagcg cgcctcctga ccaacatata
    18541 aatctggcgc attcatcacg cgtaccagat cttgctgtgg tgttggactg ctgcctttaa
    18601 tggcgtccgg cacagtgaca caaccgctca gcattagcgc aaacgtacct gccagtatgc
    18661 ctttgataac attcttttga accgccatga tgcgactcct ttttttctca gggcatactc
    18721 ttaagattca ttctttgccc ggaagtttct tccatgcgac gttgttacgt aaataaaccg
    18781 gttcggcatg ttccaccgcc accgttttac cctcggcaaa catctgacac gcaatcggca
    18841 gcatatcttc cgccgctggc agtaaaactt ctccgtcgcg caaaaccagc ccgctctctt
    18901 tacggagatc cggccaggct tgccagcccg tccctaccgt tacccattcg ccggaaagct
    18961 gttgcattcg ttcatgaacg agttcgggtt tgagtacggc ttcggtttct tcaccgcgcc
    19021 agataccgtt ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa
    19081 tggctgccag cacgcgggtt gcgccgtttt tgcgccacgc cccctgcgcc atcgtcatta
    19141 gcgtagagac accaatcatc ggtaactccg cgccaagcgc cagcccttgc gcaataccaa
    19201 taccaatgcg cacgccagta aagctaccgg ggccgcgccc gtaagccaga gcgtttatgt
    19261 cagtcaggga agttccgctg gtggtcagga tatcctgcac catcggtaag attcgttgag
    19321 tatgttcacg agggcaaagc tcaaaatgag cgttgacatt accgtcgttc cacagagcga
    19381 cagagcagac ctctgtcgcg gtatcgatag ccagaattcg catgggtctt cgtgcttaga
    19441 tcaataaaaa ggcgcgcatc ataccatact ccgtaacaaa ttacctggag gatggtatcg
    19501 caaggaaacg aaccgcacgg gcaatgtcac gggtgcgtgg cgcgggcggc agactggcga
    19561 gaaacgtcgc gccgtaagga cgcatcacca gccgattgtc acaaattacc agcacgccgc
    19621 gatcgtcggc gtcgcgaatc agtcgcccta ctccctgctt gagagtaatg accgcatccg
    19681 gcagctgcac ttcatcgaac gggtcgccac cgcgcaaacg acaatcttcc atgcgcgctt
    19741 ttaacagtag atcatccggc gaggtaaacg gcaatttgtc gataattacc aatgacaatg
    19801 tatcgccacg cacgtccacc ccttcccaga aactactggt tgccacaaga agcgcgttac
    19861 cggcgctgac aaattgctgc aacagttgcc ctttgctggt ttccccctgc aacaaaacgg
    19921 gaagcgtcat ggtagcgcgg aactgctcgg ccagatcgcg catcatggca tgcgaggtac
    19981 aaagcataaa acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt gccgccagtt
    20041 gccgtgcaca acccggttga ttggtttgcg gcaggttgcg cggcacacag agtaacgcct
    20101 ggcggctgta atcaaacggg cttggcaata gcaacgactc cgcctgttct atcccaagcc
    20161 gcgaggtgaa atgatgcaga tcgtcgttca ccgacagcgt tgctgaggtg aagatccagc
    20221 taccgggctt ttgcgccatt aactctttga atttatccgc cacgctgagc ggcgtgagag
    20281 ccagagtaaa atggcgcgaa gtgcattcgt accagtagct gtagcccggc tgattgatct
    20341 cttttagccg cttcagccgt gtgcgataca acgtggcgcg ctcaaatgcc gcatccagca
    20401 aggcggaacg ccccagtgac agtttcgcca cgtcataaca aagttccagg gtgtcatcga
    20461 gcagtaaaaa tgcccgctga atttgcggat tagctaacag ctcacgcagg ttaccgcgat
    20521 aacctggctc accgagttgc agacgaaaat cctgtgcact ctgggcaaga cgatcagcgc
    20581 acttttgtaa ctgctgggtg tcttttaatt cggtgcggta ggcgatggtg atgtcttttg
    20641 ccaggtcgag cagttgtcga ctggagagtg actgaccaaa atactggctg gcaatgtccg
    20701 gtagctggtg ggcttcgtcg aagatcatga cgtccgcttc agggatcaac tcgccaaatc
    20761 cactctcttt aaccaccata tccgccagaa agagatgatg gtttaccacc accacatcgg
    20821 cgtccatcgc ttttttacgt gctttgacca caaagcaatc tttatacatc gggcagtcgc
    20881 tgccaagaca gttgtcgttg gtgctggtga ccagcggcca cgcctgtgaa tcttccgcca
    20941 cgctgacgca ggtgctgata tcaccatcga ctgtttgatt agaccaggag cgcagcagga
    21001 tcacatcgct taagatttgt accggcagat cgccccccgc cagcgcctgc tgttcgagac
    21061 gttcgaggca gaggtagttt gagcgccctt tcagcagcgc cacgttgccc gtatatttca
    21121 atgcctttga gactgttggc aaatcgcggc tgtagagctg atcctgcaac gcttttgagc
    21181 cggtcgagat aatgactttc tttttcgccc gcagcgcagg agccaggtaa gcgtaggttt
    21241 tgcccgtacc ggttcctgct tccaccacca gcggctggcc tttttctatc gcctgggtga
    21301 cggctaccgc catctgtcgc tgtggttctc gcggcttaaa gcctggtatc gctttcgcca
    21361 gctgaccgtc tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg aacagggatt
    21421 atgtcaggat gagggcgctt tcgccagttg aagtggtgac ggcaacctca cattgtggca
    21481 gtctttgcag cacgaaaatg gaagtataat gaggacaaaa tgatgactat cgttcgtatc
    21541 gatgctgaag cccgctggtc tgatgtagta atccacaaca acacgctcta ctacactggt
    21601 gtaccggaaa acctcgacgc cgatgccttt gagcaaaccg ccaacacgct ggcacagatt
    21661 gacgccgtgc tggaaaaaca gggcagcaat aaatcgagca ttctggatgc caccattttc
    21721 ctggccgata aaaacgactt cgcggcgatg aataaagcgt gggatgcctg ggttgtcgcg
    21781 ggtcatgcgc cagtgcgctg cacggtacaa gcgggtttga tgaacccgaa gtataaagtt
    21841 gaaattaaga ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc agacgataat
    21901 ttatcgataa tactggtcgg ttttacataa atcgaccaga gaataagata ttactgttga
    21961 tgcttctgct aattcattat ttatatttat aatttcaatt ttatctataa aactaactat
    22021 ataaataaac ataaccatgc cgttgtgttc gccctttgat tagcaactct ggttttcttt
    22081 cttagctgaa caatctggtt atagcgtgcg gactgaccgt taattttctc cttcttactg
    22141 gataacacac catgagctgc tacctttaaa acacctgaat gtcaacaggt taactcgtgc
    22201 catatcgtta taatcccttt gatattagat gcaaatta
//
LOCUS       DAZPEP010000056        21972 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZPEP010000056 DAZPEP010000000
VERSION     DAZPEP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21972
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.056"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..330)
                     /locus_tag="ACHUT9_002127"
     CDS             complement(<1..330)
                     /locus_tag="ACHUT9_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFR1348600.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIYMNASRLQGIHCFDMTGRLQLYIRLYCSRNAA
                     SPYVIRRL"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUT9_002128"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="ACHUT9_002128"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFR1348601.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUT9_002129"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="ACHUT9_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFR1348602.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUT9_002130"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="ACHUT9_002130"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFR1348603.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUT9_002131"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="ACHUT9_002131"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFR1348604.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUT9_002132"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="ACHUT9_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFR1348605.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUT9_002133"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="ACHUT9_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFR1348606.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUT9_002134"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="ACHUT9_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFR1348607.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUT9_002135"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="ACHUT9_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFR1348608.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUT9_002136"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="ACHUT9_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFR1348609.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUT9_002137"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="ACHUT9_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFR1348610.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUT9_002138"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="ACHUT9_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFR1348611.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUT9_002139"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="ACHUT9_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFR1348612.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            13116..13544
                     /gene="uspC"
                     /locus_tag="ACHUT9_002140"
     CDS             13116..13544
                     /gene="uspC"
                     /locus_tag="ACHUT9_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFR1348613.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13551..14975)
                     /gene="otsA"
                     /locus_tag="ACHUT9_002141"
     CDS             complement(13551..14975)
                     /gene="otsA"
                     /locus_tag="ACHUT9_002141"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFR1348614.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(14950..15750)
                     /gene="otsB"
                     /locus_tag="ACHUT9_002142"
     CDS             complement(14950..15750)
                     /gene="otsB"
                     /locus_tag="ACHUT9_002142"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFR1348615.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            complement(15917..16903)
                     /gene="araH"
                     /locus_tag="ACHUT9_002143"
     CDS             complement(15917..16903)
                     /gene="araH"
                     /locus_tag="ACHUT9_002143"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFR1348616.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(16918..18432)
                     /gene="araG"
                     /locus_tag="ACHUT9_002144"
     CDS             complement(16918..18432)
                     /gene="araG"
                     /locus_tag="ACHUT9_002144"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFR1348617.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(18502..19491)
                     /gene="araF"
                     /locus_tag="ACHUT9_002145"
     CDS             complement(18502..19491)
                     /gene="araF"
                     /locus_tag="ACHUT9_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFR1348618.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            20288..20791
                     /locus_tag="ACHUT9_002146"
     CDS             20288..20791
                     /locus_tag="ACHUT9_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFR1348619.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(20870..21121)
                     /gene="yecJ"
                     /locus_tag="ACHUT9_002147"
     CDS             complement(20870..21121)
                     /gene="yecJ"
                     /locus_tag="ACHUT9_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFR1348620.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(21236..21322)
                     /gene="azuC"
                     /locus_tag="ACHUT9_002148"
     CDS             complement(21236..21322)
                     /gene="azuC"
                     /locus_tag="ACHUT9_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFR1348621.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            21585..21908
                     /locus_tag="ACHUT9_002149"
     CDS             21585..21908
                     /locus_tag="ACHUT9_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFR1348622.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT         5370 a         5840 c         5177 g         5585 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagtg acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg acgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag cacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat ggcacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
    12361 tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg taaaaaataa
    12421 ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt ggtttaatat
    12481 gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa atcgacgcaa
    12541 ctgtactcgt cactacacgc acatacaacg gagggggggc tgcgattttc aataatgtgt
    12601 gatgcagatc acacaaaaca ctcaattact taacataaat gtataagtca tacttttgtt
    12661 ttgggtgtat ttcaatatgt taaaaagttt ttcgctacgc tagcaagcaa aaatgaaaca
    12721 ggaataatcg aaatgggatg ttgcgcacag ttaaaataaa tcaccgtaaa taatcatctg
    12781 ctataaataa tcactttcat gcaataccag ataagctatt tttaaacaga cacttaccgc
    12841 acaacaaact aattaaatac aaagaaaggc aacaatcaac ttaatgttaa tgaaatgttt
    12901 tacatatatt aaccacttgt ttaatgcgga gaatcagtac actattttct tataattaca
    12961 tttgaaatta tatgatcacc gggagtgatt gattaattgt ctttactaat aatcagacta
    13021 atatttacct gtttgaccga gttgggattg cgtcgtttct ccattaggag taaagcttta
    13081 atgtcacctg aagttcacag aataaggaac aggttatgag ctatagcaat attcttgtcg
    13141 ctgttgcggt tacaccggaa agtcagcaac tgctggcaaa agcggtatct atcgccaggc
    13201 cagtaaaggg acacatcagt ttaattactc tcgcttccga cccggaaatg tacaatcaat
    13261 tagccgcgcc gatgcttgaa gatttacgca gcgtgatgca agaagaaacg caaagctttc
    13321 ttgataagtt aattcaggat gcggggtatc ccgttgacaa gacgtttatt gcctacggtg
    13381 agttaagcga acatattctg gaagtatgtc acaaacatca tttcgatttg gtgatttgcg
    13441 gtaatcacaa tcacagtttc ttttcgcgag catcctgctc ggcgaaaaga gtgattgcct
    13501 caagtgaggt cgatgtgctg ttagttccac ttacgggaga ttaaccgctc ctacgcaagc
    13561 tttggaaagg tagcaacttt atcgcgctgc tggctttccg cgcttcgcgg aactatctgc
    13621 tttaggtcgc taatgaagca ctcctgccag tggttaatat cgtttttcac gataacgtcc
    13681 agcatttctg catgacggga aatacgttcc gccagcgaca tagtcaatgc acgatccagc
    13741 gcagctgcaa cttcgtcacg atcgtagggg ttaacaatta acgccgacgt taactcgttt
    13801 gccgctcccg caaattgcga aagaacaaga acgcccgggt tggctgggtc ctgagcagca
    13861 acatactctt ttgctaccag gttcatcccg tcacgcagtg gcgtcactaa gcccacgtca
    13921 gagtagcgga atattttcat cagtaattta cggtcaaaat gctgattcaa ataataaagc
    13981 ggcgtccagc ctaattgccc gtatttacca ttaattcgtc cagcttcatt ttcgagctga
    14041 tgacgaatat cctgataggc ttgcacatca ccacgcgacg ttggtgcaat ctgggtataa
    14101 cgaattttac catgatgctg cggatatttt tccagcaacg cttcataggc gagaaaacgc
    14161 tctggcaaac ctttggaata atccagccgt tcgacagaaa agatattttg tacgtttttc
    14221 agttccgctt taagttgcgc cagttttggc ggcagtggcc cggcagcctg tttggctatt
    14281 tctttcggtt caatgccgat cgggtagact tctgttcgaa atgctttgcc ccaggctgta
    14341 tggctttttg cgctacgtgt cgtgacgcgg gtcaggttag aaagacaatc caggaacgcc
    14401 agacgatcgt tttctgtctg gaaacccagc aaatcataat cacaaagctg ttcaagcaag
    14461 gtgtcatatg tcggcagcgc gttgaagatt tccggtgtcg ggaaaggaat atgcagaaag
    14521 aaaccaatgc gattattcac tccccgtttg cgtaattcat gcgcaaatgg caacaggtga
    14581 taatcgtgga tccagataat gtcatcgtct tgcaacagcg gcagtaattt atctgccagc
    14641 aacgcattta cgcgtagata gccgtcccag gcaggacgct gaaattgcac cagatcgagc
    14701 cgataatgaa aagcgggcca gagaacggca ttggagaatt ggttgtagta ttcgtcaagg
    14761 tcctgttcgc tgaggttaaa agaggcccac gtaatgttac cttttttcac cttttttagc
    14821 ggctgatcct cattccctgt ttcaccactc cagccaaacc acagtccgcc tgcggctttc
    14881 agtgccccca gtatgccaac ggcaaggcca ccggcactgg cggcgtgctc gtctggtggt
    14941 gcaatccggt tagatactac gactaaacga ctcatagtca tcactcctgt tattttctct
    15001 tttttgttgt aatgcggtgg ttatcatttc aagccagctc cagacatccg gcacacccgc
    15061 cagtcgccat gatgcctgag ttgcacctgt gccaattttt actgacattc cgcccagtcg
    15121 gttaacgact gcgaagccag attcatcggt taaatcatcg cccagaaata cgggcgttcg
    15181 cccgataaag ggagcttcct gcataaaagc tgcaattgcc tcacctttac tggtacctct
    15241 cggtttgatc tcgacaacac actttccctg ctgtaacgcc atttgtggcc agatctgagt
    15301 aatacgttgc gctaatgtca ttaatgcgtc ttcatgctgc ggagcctgac gataatgcag
    15361 cgcaaaagcc atccctttcg cctccagctc cgcgccggga tactgagcga tgactgtatg
    15421 cagttgcacg ctaatatcac gcgcaatcgc atccggcaga tgaacgatat gtgttttacc
    15481 attgatgtca cggcgctccg ccccatgcac gcccgctaac gggaagcgat aaggttttgc
    15541 cagtgcgtca agctccacca ttgagcgccc tgatatcaat gccaatgcac catcacttgc
    15601 ggttgccagt agctgtagtc cttgcagaat attgtcaggc acgacgacct gatcgggatg
    15661 cggtttgatt tccgccagcg ttccatcaag atcaaaaaac caggcatatt tcgcggatag
    15721 ttcaggggtt tcggttaacg gttctgtcac ccggctctcc tccttctctt ttcttattgt
    15781 tttgcgcaga acgcgcttta cagacatgta agtatagaca gtgtgacggg ggtcgccatt
    15841 tgcaatgaag aacagccaac cgttgtggcg gctggctgtg gtgggaaaaa acgttaaatt
    15901 gttgtggaaa aaagcatcag acagtgcgtt tcgctttttg cttgtaacgg tcgaagatca
    15961 ccgctgccag caggattaag ccgcgaacca cgtactgcgc gaaaggagaa atattaagca
    16021 ggttcatggc gttttccacg gtgcctaaaa ttaagatacc cgccaccaca tatgagattt
    16081 ttccgatgcc acctttcaga gaaacgccac ctaaaacgca ggcggagata acaataagct
    16141 cataaccaat cgacgtcatt ggctgcccac tagtcatacg tgaagccaga ataattccgg
    16201 ctatcgcaga taccaggcca gagagaacaa agataataat tttggtgcga acaaccggta
    16261 cacccgccag acgcgcagcc tcttcgttcc cgccaatcgc cagggtgtta cgaccaaagg
    16321 tggttttatt cagcaacaaa ccaaagataa tcagacacgc gacggtgagc cagattggcg
    16381 caggcagacc gaaccagtta gcgtaaccaa gggcaaagaa gctttcatct tcgataccga
    16441 ccgctttacc gtctgaaatg atgtacgcca gacctcgaac aatctgcatc gttgccagtg
    16501 ttgtgatcag agcatttatt ttcagtttgg cgataacaaa gccattgacc aggccacaga
    16561 gaatgccaag cagcaacccc gctgccacgc caatccacag gctttcagtc aggttgataa
    16621 ccaccgccgt ggtgacaccc gcacaggcaa ttacggaggc gacagaaagg tcaaagtcac
    16681 cggaagcaag gcagaacaac atgccacacg ccaccatccc cgacatggaa attgccaggc
    16741 ccaacccttt catattaatg aaggtggcaa aatttgggac aaaaatggca caggcgataa
    16801 agagcaccgc aaacaccacc agcatgccgt actgatccca aatacgcccg aagctgaatg
    16861 acgacttagg tgcgccagaa cccgatgtag aaacagaaga catcatactc tccttactca
    16921 ggcaactgcc tggctgactt taggcatcgc aaggctcagt gcctgacgct catctgcctg
    16981 ctcgtgtaac aattcaccgg cgatttcacc ttcccgcatc accacaatcc ggtcggcaac
    17041 gccgaggact tcaggtaagt cgctggaggc aaacagcacc gccacaccct gcgccgccag
    17101 cgcataaatt acgttatata tttcgtgctt agcaccaaca tcaatgctgc gcgtaggttc
    17161 atccagcaaa atgaccttca tctcttccga taaccagcgg cccagaatgg ctttttgctg
    17221 atttccgcct gagagattca tgatcagttg ctcagcgccc ggcgttttga tgttgagcga
    17281 acgaatgtgc tgatcggcat tgttttcttc ccaaccgttg ttgattacac aaccgccgag
    17341 cacatgtttg cgtctggcac tgatgttgat attgtcgcga acggagtgca cgggaataat
    17401 gccttccgct ttgcgatctt ccgggcagag catcatgcct gcggcaatgg cgtggctcgg
    17461 tttacgaata tcgatcggct gttggtcgat ataaacctga ccggcggtga tttgcgtccc
    17521 gccaaacaag cctttcatta attcgctacg ccccgcccct accagaccaa acagcccaac
    17581 aatttcacca ctgcgaaccg ccagacttat tggcgtacgc acgcctggtg ctttcacagc
    17641 atcaagacgc aggcgctcct cgccataact acgaggttgc cagccgtaga tatcgccaat
    17701 gtcgcgcccg accatcgcct gcaccagcgc gtcgtggtca acctgctgca tatcggtaaa
    17761 ggttttgaca taacgtccat ctttaaagac agtgatggca tcgctgaggg caaatatttc
    17821 ttccatacgg tgagaaacgt ataagatcac ccgcccctct tttcgcagtt cacgaataac
    17881 gcggaaaaga ttgtcgattt cgcgggcaga aagggagctg gttggctcat caaaggcgat
    17941 aattttagcg ttacgcgcca gcgccttggc gatttcaacc atctgccact gaccaatgga
    18001 gagatatttc agcggcgtgt ccgggtcaat atccatacca agatgtttaa gttgtaaacc
    18061 cgcctcataa ttcagcaatg agcgattcac aatgccgcct ttatgcggca gctggccgag
    18121 atagatgttt tccgcgacgg tcatttccgg cacgagatgc agttcctggt aaataatcgc
    18181 cacacccgcg ttaagtgctg cggtcgtgtc ggaaaaggac atttcctgcc cattaatcac
    18241 tacagaaccc gtggttggcg catagttgcc gctgaggatt tttaagagag ttgattttcc
    18301 tgcgccattt tcacccatca acgcatgaac ctgaccggca tagcagtcaa aactaatatc
    18361 cgtcagcgcc ttaacgccgg gaaacgtttt accgatgccg cgaaatgaga gatacggggt
    18421 agactgttgc ataacgtctc cgtgaatcac tggtcgttca caccccctct gcatcatgca
    18481 gaggggaatt tttccggcaa attacttacc gcctaaacct tttttctcca gttcttcttt
    18541 aaagttgtca cgcgtgatca gcaccacgtc ggtaacttcg gtaaattttg gcggttcaac
    18601 gtcttttgct acccagttgt aaagcatttc gctggattta tagccatgta cgtccgggct
    18661 tggcagcagg gaaccgtaga agccggttgc ctgtgcttta gacagttcgc tcaacgcatc
    18721 cacaccgtta atgccaatgc cgatgatatc ggcagcttta aagccctgac cttccgtcgc
    18781 gcgtacgccg cccagcacgg tgctgtcgtt cataccgacg atcagccaat gtttaacttc
    18841 cggatgttga accagcattg agttggcagc gtcaaatgcc cccgggatgt cgttagattt
    18901 ggtaggtacc tgataaattt gtttttccgg gaatccggcc gctttcagcg catccataga
    18961 acccgttgta cggcgacgtg ccgtatccag ttcgttggcg gtaatcgcca tcaccgcgct
    19021 ttctttgaca tcccagccac gtttctgcat ctctttatac agttcctgac cctgacgttc
    19081 gccaatttta gtcgccgcca tcatcaccag cggaacggta tccattggct tacctttggc
    19141 gttaacaaac tggtcatcca cggcaatgac tttcatatcg tagccacgcg ctttcgcgac
    19201 gatggcagag ccgagtttgg ggtccggagt acaaataacg aaaccttttg cgccactggc
    19261 agccaggctg tcgatcgcgt tcaatgtttt ttcgccatcc ggcacggcaa tcttaataac
    19321 ctcaaaccct aaatccttcc cggctttatc ggcaaacttc cattcggtct ggaaccacgg
    19381 ctcttccggt tgcttcacca gaaaaccgag cttcaggttc tccgccatag cggattgtga
    19441 cataacggct gccagaccaa tggctgccag ggctttagta aatttgtgca tggttctctc
    19501 cagctttagt gtcgttttgt gtagggcaaa aacgaatgac tttcgttaaa ttaatcggaa
    19561 aacaaagcat taccttttat ctaaaagata agtgaccgtg ttgacatagt tttagcgaga
    19621 aattaattct ccataggaga gcaatatcac atcgcagaat tacagtgaga acgtgcataa
    19681 atttagcggg aaaagacata agggaaagcc aatttgtcag acaaattgtc gaatgcacag
    19741 cagattaatc cataagatta gcctggaaat ccttgttgtc tttggtaccc atgcgggatg
    19801 tcttcttttt aaccagtcaa taggccgcat tacctggcgt tgagtttttg aaatggtgta
    19861 ataaccgcaa ctcaaagatg tggaaaatgc acgtcattca tttcgccatt aattatcact
    19921 gtgctcatta attaacagaa cacgtataat gagagccatc tcgcaaaaat gaaaaaacgt
    19981 tttataaaat catcacttca tcatgaattc aaattcattg attaatatca acaagataca
    20041 aaaagcacta tcattaaaat tcattgcagt tacattgatt tcatcaatga aatgtaaaaa
    20101 tatataaact tgatgattta agcattttct tatacccgtt cagacgttat tcttatttca
    20161 gatcatcgtc agaattgact ccacgatcac atttcggacc ggcagaaagg aattattctg
    20221 caaacagtaa ttatggtgtt ttgatttatc ttgcacctct ccacttctgg atataaggat
    20281 attaggtatg gcaaccgctg gaatgcttct caaactcaac tctcaaatga accgcgagtt
    20341 ttacgcatcc aatctctacc ttcacctgag taactggtgt tctgaacaga gtctgaacgg
    20401 caccgccact ttccttcgcg cccaggcaca gagtaatgtg acccaaatga tgcgcatgtt
    20461 taactttatg aagagtgtcg gcgctacccc catcgttaaa gccattgatg ttcccggtga
    20521 aaaactgaac tctctggaag aactgttcca aaaaacgatg gaagaatacg agcaacgttc
    20581 tagtacgttg gcacagttag ccgatgaagc gaaagaactg aatgatgatt caaccgtcaa
    20641 tttcctgcgc gatctggaaa aagaacagca gcatgatggt ctgttgctgc aaaccattct
    20701 tgatgaagtg cgcagtgcga aacttgcggg tatgtgccct gtgcagaccg accaacatgt
    20761 tctgaatgtc gtgtcacacc agctgcattg atcatcatcg gcgctaatgc attgcgccga
    20821 tgaaggtttt gagaaaccgc tgcctcatct gtttgaagca gcggtttttt taatgggatt
    20881 caccctgtgg ggtaaacttg agttcaataa gcgcgatggc tttttggatt gcccgcatgg
    20941 tgaccgggtc tgcggcggcg ggatggttag taaagtcgat attcttcagc tgactggaca
    21001 ttttttcacg aacttcaacg ggcgcgatta catcgagaac atccagaatt tgtttgataa
    21061 ccaactggca agcaaccaca tcagaaacca gttcctgatc ggcattcagc ggctgggaca
    21121 tcgtaaactc ctgatagcat tttgaaagcc gttatagtag cggcttcaca tcttcagcga
    21181 tagtcacatc caccgtcatc aggacacaaa aaaacctgcc ggagcaggtt ttttgttatc
    21241 ggaacatatt gcctggcggt acgtctttga acgtcttgca atagttattg aacatacttt
    21301 tcaggatttt gcgcagtttc atcgcggcac tccgaccatt tgttatacag gtgttattgt
    21361 ctttgcgctt ataatatgac aaccatcaca aaaatcaatc tttatgtgat acaaatcaca
    21421 taaatacccc tttaatgtta taaaaatgat aatcaaaaaa cagcccccct atttctgaca
    21481 cctacagatg gcaagaaata gcgcctgcca ggcgtctttt ccggccattg tcgcagcgct
    21541 gtaacgcgta aaatagtgct ttctcttact cttctggctg gaccatgaga cttctgattc
    21601 tgactctttc attaataacg ctggcggggt gtacggttac tcgccaagcc catgtgagcg
    21661 aagttgacgc ggcaacaggt attgtacggt tggtttatga ccaggctttt ttgcagcatg
    21721 cccataccga tcgttatgtc agtcgcggga tcgccgatcg cgcatgccag caggaaggct
    21781 atacccacgc ggtccccttt ggtcagccag taggcaactg tagccttttt gcgggttcct
    21841 tatgtttgaa tacggaattc actttgtcat atcagtgcca tcattccgcc ttccctgtct
    21901 ttctctaaat attttaatca gcgaggggat cttcgctgat taaagaaata gcggtaatgc
    21961 gttttattcc ca
//
LOCUS       DAZPEP010000057        21642 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZPEP010000057 DAZPEP010000000
VERSION     DAZPEP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21642
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.057"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUT9_002150"
     CDS             complement(<1..1126)
                     /gene="fsr"
                     /locus_tag="ACHUT9_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFR1348623.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUT9_002151"
     CDS             1344..2996
                     /gene="ushA"
                     /locus_tag="ACHUT9_002151"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFR1348624.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUT9_002152"
     CDS             complement(3033..3512)
                     /gene="ybaK"
                     /locus_tag="ACHUT9_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFR1348625.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3716..4510)
                     /locus_tag="ACHUT9_002153"
     CDS             complement(3716..4510)
                     /locus_tag="ACHUT9_002153"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFR1348626.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4648..4989
                     /locus_tag="ACHUT9_002154"
     CDS             4648..4989
                     /locus_tag="ACHUT9_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFR1348627.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUT9_002155"
     CDS             complement(5204..7708)
                     /gene="copA"
                     /locus_tag="ACHUT9_002155"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFR1348628.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUT9_002156"
     CDS             7970..8902
                     /gene="glsA"
                     /locus_tag="ACHUT9_002156"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFR1348629.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8905..10197
                     /locus_tag="ACHUT9_002157"
     CDS             8905..10197
                     /locus_tag="ACHUT9_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFR1348630.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10228..10325
                     /locus_tag="ACHUT9_002158"
                     /pseudo
     CDS             10228..10325
                     /locus_tag="ACHUT9_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUT9_002159"
     CDS             10322..10729
                     /gene="cueR"
                     /locus_tag="ACHUT9_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFR1348631.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10730..11188)
                     /locus_tag="ACHUT9_002160"
     CDS             complement(10730..11188)
                     /locus_tag="ACHUT9_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFR1348632.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11185..12102)
                     /locus_tag="ACHUT9_002161"
     CDS             complement(11185..12102)
                     /locus_tag="ACHUT9_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFR1348633.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUT9_002162"
     CDS             12248..12925
                     /gene="fetA"
                     /locus_tag="ACHUT9_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFR1348634.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUT9_002163"
     CDS             12912..13691
                     /gene="fetB"
                     /locus_tag="ACHUT9_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFR1348635.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUT9_002164"
     CDS             complement(13754..14608)
                     /gene="cnoX"
                     /locus_tag="ACHUT9_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFR1348636.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUT9_002165"
     CDS             complement(14669..15478)
                     /gene="ybbO"
                     /locus_tag="ACHUT9_002165"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFR1348637.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUT9_002166"
     CDS             complement(15468..16091)
                     /gene="tesA"
                     /locus_tag="ACHUT9_002166"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFR1348638.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUT9_002167"
     CDS             16062..16748
                     /gene="ybbA"
                     /locus_tag="ACHUT9_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFR1348639.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUT9_002168"
     CDS             16745..19159
                     /gene="ybbP"
                     /locus_tag="ACHUT9_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFR1348640.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUT9_002169"
     CDS             complement(19393..20487)
                     /gene="mnmH"
                     /locus_tag="ACHUT9_002169"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFR1348641.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUT9_002170"
                     /pseudo
     CDS             complement(20556..21482)
                     /gene="allS"
                     /locus_tag="ACHUT9_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5014 a         5686 c         5799 g         5143 t
ORIGIN      
        1 gatcggcgat aagccccaga actgccgctc ccagacctcc catgccaaaa gcaaaaccga
       61 aaaagagtcc agaaaccata ccgatacgtc ccggaagtag ctcctgagcg tagaccagaa
      121 tggcagagaa tgccgaagcg aggataaatc caataatcac cgttaaaacc cccgtccagt
      181 gcaggctggc gtagggtaaa atcagcgtaa acggcgcaac gccgaggata gagccccaaa
      241 tcacatattt ccgtccaatt ttatccccta caggcccgcc gatcaccgta cctgccgcaa
      301 cggcaaacag gaaggcaaac agatgaagct gggcattctg gatagataat ccgaattttt
      361 gcatcagata aaaggtgtaa tagctgctga tgctcgccat atagaaatat ttcgagaaaa
      421 tgaggattaa cagaatgctg actgccagta ccactttgtt gcgcggcagt ggattgatta
      481 tcgtcgcttt gggttttcct ttattcattc ggtgctgtgc cgagtaccaa cggctaattt
      541 gcgccaacac cacgatcgcc agcagtgccg caagcacaaa ccaggcaacg ttgcctttgc
      601 cataaggcgc gataatcacc gccgccagca agggtcccag ggaactgcca aagttgccgc
      661 cgacctgaaa gatagattgc gccaggccat gccgcccgcc ggaagccata cgggctacgc
      721 gagaagattc cggatgaaag accgatgaac cggtaccgac cagcgccgcc gccagcagaa
      781 ctgcgccaaa actgcccgcc agcgcaagca gcaccagacc acttaaggta aagcacatgc
      841 caattggcaa cgaccacggc atcggatatt tatcggtcca gtagccgacc actggttgca
      901 gtagcgaaga ggcgagctgg aaggtgaggg ttatcatgcc aatctgcata aatgtcagag
      961 aaaattctga ctgaagcagc ggataaatcg ccagaatcag cgattggatc atgtcgttca
     1021 gcagatgtga gaggctgata gcacctaaaa taccaaacga tgttcgggcc ttggtcgttg
     1081 acgcagccgc gcccgccaca ggctggggtt gttcactcat tgccatagga aagtcacttt
     1141 ttcagggttg cgatgtaaag aatgatctta tttgtgatta ttacccgact aacatacctg
     1201 tatgcgtcgt ctgaaggaag tctcaacgcc gaatacagaa tttctaatct ggatgcagat
     1261 ttatcttcac cggacgcaga cttgtctatg atgtcgcgtc atactatttt tcaacacgtt
     1321 gaaatcaggt cagggagaga agtatgaaat tattgcagcg gggcgtggcg ttagcgctgt
     1381 taaccacatt tacactggcg agtgaaactg ctctggcgta tgagcaggat aaaacctaca
     1441 aaattacagt tctgcatacc aatgatcatc atgggcattt ttggcgcaat gaatatggcg
     1501 aatatggtct ggcggcgcaa aaaacgctgg tggatggtat ccgcaaagag gttgcggctg
     1561 aaggcggtag cgtgctgcta ctttccggtg gcgacattaa cactggcgtg cccgagtctg
     1621 acttacagga tgccgaacct gattttcgcg gtatgaatct ggtgggctat gacgcgatgg
     1681 cgatcggtaa tcatgaattt gataatccgc tcaccgtatt acgccagcag gaaaagtggg
     1741 ccaagttccc gttgctttcc gcgaatatct accagaaaag tactggcgag cgcctgttta
     1801 aaccgtgggc gctgtttaag cgtcaggatc tgaaaattgc cgttattggg ctgacaaccg
     1861 atgacacagc aaaaattggt aacccggaat acttcactga tatcgaattt cgtaagcccg
     1921 ccgatgaagc gaagctggtg attcaggagc tgcaacagac agaaaagcca gacattatta
     1981 tcgcggcgac ccatatgggg cattacgata atggtgagca cggctctaac gcaccgggcg
     2041 atgtggagat ggcacgcgcg ctgcctgccg gatcgctggc gatgatcgtc ggtggtcact
     2101 cgcaagatcc ggtctgcatg gcggcagaaa acaaaaaaca ggtcgattac gtgccgggta
     2161 cgccatgcaa accagatcaa caaaacggca tctggattgt gcaggcgcat gagtggggca
     2221 aatacgtggg acgggctgat tttgagtttc gtaatggcga aatgaaaatg gttaactacc
     2281 agctgattcc ggtgaacctg aagaagaaag tgacctggga agacgggaaa agcgagcgcg
     2341 tgctgtacac ccctgaaatc gctgaaaacc agcaaatgat ctcgctgtta tcgccgttcc
     2401 agaacaaagg caaagcgcag ttggaagtga aaataggcga aaccaatggt cgtctggaag
     2461 gcgatcgtga caaagtgcgt tttgtacaga ccaatatggg gcggttgatt ctggcagccc
     2521 aaatggatcg cactggtgcc gactttgcgg tgatgagtgg aggaggaatt cgtgattcta
     2581 tcgaagcagg cgatatcagc tataaaaacg tactgaaagt gcagccattc ggcaatgtgg
     2641 tggtgtatgc cgacatgatc ggtaaagagg tgattgatta cctgaccgcc gtcgcacaaa
     2701 tgaagccaga ttcaggtgcc tacccgcaat ttgccaacgt tagctttgtg gcgaaagacg
     2761 gcaaactcaa cgacctgaaa atcaaaggcg aaccggtcga tccggcgaaa acttaccgta
     2821 tggcgacatt aaacttcaat gccaccggcg gtgatggcta tccgcgcctt gataacaaac
     2881 cgggctatgt gaataccggc tttattgatg ccgaagtgct gaaagcgtat atccagaaaa
     2941 gctcgccgct ggatgtgagt gtttatgaac cgaaaggtga ggtgagctgg cagtaatccg
     3001 aaagtgccgg atatttgcat ccggcacaat gcttaatcgc ggcgggcgat atcagcaaat
     3061 ttggcatcga ggatcttcgc cagatcgcct gccgccagtt cgatatccag tccgcgcttg
     3121 ccgccagaaa cataaatagt ggcaaattct tgtgcggggg cgtcgataat cgtcggcaga
     3181 cgttttttct gccccagtgg gctaatcccc ccaaccaggt atcccgtcga acgctgcgcg
     3241 gccatcggat cggccatctc aactttcttg gcacccagcg cttttgctac ttttttaaga
     3301 tccagttgac ctgcgaccgg cgtaacggcc actgccagat gtttcatatc accgttcact
     3361 gccaccagta gcgttttgta gacttgatcc ggattcaaac ctaatttttt gacgacttca
     3421 tcgccaaaat tggtttcagc cggatcgtgc tcgtaggtat ggatttgaaa cgaaatcttg
     3481 tttttttcga gtaatttaac tgcgggtgtc atggtaattt tcctgcctta aacaaaaaaa
     3541 cgaagcaagc atacgcctga tggcggtcca aaaaaaagag tcatcttgcc taagagtatt
     3601 ggcaggatgg tgagattgag cgacaatcga gttacaccgt cgcttaaagt gacggcataa
     3661 taataaaaaa atgaaattcc tctttgacgg gccaatagcg atattggcca tttttttagc
     3721 gcaacatttg cggcaaattc ccttctccat acaggtgtag tgcaccgacc gcgaccacat
     3781 atcgccccgg cggcatggcg cgtaatttat cccgccaggc gagatttcgc tgatgcatca
     3841 gcacatcgta cagcgactga ctgaacgtat tgggcagcgt tatatcatta ttttgcggag
     3901 gtgcattcag ccaccagctc atcatttgtt gcagcaaccg tgcgttggta tgccagtggg
     3961 tcagcgtatc gtccagcagc gccagtcctt tgtcagggag ctggagcaac atggtaatct
     4021 ggttttcagc cccttccagt tcaatcacgg gtttatgttg ttgcttcgcc gcctgcaata
     4081 actggtaatc gataccgtat tctgcccgca gccccagttt ttgtgcctgc gtcgcctgaa
     4141 gaaccatcgc gatttgccac agcggttgag tagaaaagag cgacggagaa atgcccatct
     4201 cctggctaat gtgctgcaga ttttggagtt gctcctcgtt aatgcgctct tccagcgcct
     4261 cgcaagtagg caaattagca aaaggcgtat cgctggtgga aacatccgcc tcgacgatca
     4321 gcgcatcggc gtttttgagc tttttgagca aacgggtggg caggggagcc atatcgtggc
     4381 tacccatatg aatactgcca atcagatgaa aatggcgatt gccgggaagg gtgatatcga
     4441 tggcaggcca ggtgtaatga ttaccgcgca gcgcggccca aagcgttttt acccggtaca
     4501 acagatccat acgacctccc tttgtgaaat atcatgctag cgcgcggtga gggatggcgc
     4561 aaatggagtt ggggacttgt ttaatgtgtt tgtatgattc agtatgttct tgcatcgcta
     4621 ttcacaggga agcaacagtt aaaaaccatg aaacaggcaa caagaaaacc gacgacaccc
     4681 ggagatattc ttctctatga atatctggaa ccgctcgatt tgaaaatcaa tgagttagca
     4741 gagttgctgc atgttcatcg taatagcgtc agtgcactga tcaataacaa tcgtaaactc
     4801 accactgaga tggcattccg tctggcgaaa gtttttgata ccacagtcga ttttaggcta
     4861 aacctccagg cggcagttga tctttgggaa gttgaaaaca acatgcgcac ccaggaagaa
     4921 ttgggacgga ttgaaacagt ggctgaatat ttggcacgcc gtgaagagcg tgcaaaaaag
     4981 gtcgcgtaag gccaaaacat cgcttcatct cgtaggccgg ataaggcgtt cacgccgcat
     5041 ccggcatccg agcaccattg cctgatgcga cgcttacccg tcttatcagg cctacaaacc
     5101 tgtgcctgaa ccgtaggccg gataaggcgt ttacgccgca tccggcagtc atgcgtcgat
     5161 gccaaatgcg ccgccctaaa gcagcgcatc cgcaatgacg tacttattcc ttcggtttaa
     5221 accgcagcaa ccggttggcg ttactcacta cggtaatcga cgagagcgcc attgccgctc
     5281 cggcaactac cgggttaagc agtgttcccg taaacggcca caaaataccg gcggcgaccg
     5341 gaataccgat gctgttgtag ataaaggcac cgagcaggtt ctgcttcatg ttgcgcagcg
     5401 ttgcgcggga aatcgcgagc gcatccgcaa cgcccatcag gctatggcgc atcagggtaa
     5461 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaatgccg acatccgcct
     5521 gggccagcgc tggcgcgtcg ttaatgccgt cgcccaccat tgccacctga cgtccttcac
     5581 tttgcagacg tttgatcgct tcggctttac cgtccggcag cactccggca atcacttcat
     5641 caattccagc ttctttggcg atcgcattgg cggtggttgg gttatccccg gtcaacatca
     5701 ccagacgata tcccgtttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
     5761 gatcgcgtac agccagcagg gctactgctt tcccgtcaat cgccagcagc acaggcgttg
     5821 ctccttgcga tgcctgagca gtaatctccg cttcgatagc tttggtacca acctgttgct
     5881 catttaacag cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
     5941 ccagcccgcg caatgtgcgg aaaccgttga cctgcggcag ctgcatatca cctgctttat
     6001 cgaggatcgc ccgtgccagc ggatggctgg aaccttgctc cagtgccgcc gccagacgca
     6061 atgcctgcgc ttcatcaaca tcagcaaacg ttttcactgc gacaacctgc ggcttccctt
     6121 cggtcagcgt cccggtttta tcgaacacga cggtatcgag tgtactggcg cgttgcagcg
     6181 cgtcggcgtc ccgcaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
     6241 acatcggcgt cgccagcccc agcgcacacg gacaggcgat aatcagtacc gtggtggcaa
     6301 tcaccagggt ataaacaatt tgcggtgccg gaccaaagaa gtaccagatt gccgcactga
     6361 ccagcgcaat caccaccacc acaggtacaa atacggctga gattttatcc gccagctgac
     6421 cgatttctgg cttgctgctc tgggcctggc gcaccatgcg aatgatccgt gacagcgtgg
     6481 tatggctgcc aaccgcactg gcacgaaaca gcacactgcc gtcctgtacc actgtcccgg
     6541 catggacgct ttcgccttcg cctttttgct gtgggattgg ttcgcccgtc agcatcgctt
     6601 catccagcca tgcttcgccc tgggtaatct cgccatctac cggcacgcga tcgccggtcg
     6661 tcaggcgcag taacatccct ggctgaactt ccgccagcgg cacgcttttt tcaccatctg
     6721 cggtgaccac tcgcgccgtc ggtggggtta aatcgagtaa tttctccagc gccttagaag
     6781 aacgctgacg tgcgcgcgct tccagcatat ggccgagatt aatcagaccg ataatcatcg
     6841 cactggcttc atagtaaaga tgccgcgctt ccatcgggaa ccactgcggc cacaggttga
     6901 cgctcatcga atagagccac gccacacccg ttcccagcgc caccagcgta tccatcgtcg
     6961 ctgcaccgtt cagcaggctt ttccatgcac tgcggtaaaa atggccgccg gcgaaaacca
     7021 tcactgccag ggttatcagg ccgataacta accacaagct gcggttgtca gcggtgacca
     7081 tcatgttatc gccgatcatc ccccagacca ttaccgggat acccacagcc agtgcaacaa
     7141 ttgcctgcca gcggaagcgc ttcatcgtag ctacggcggt ttcttgctgg cgctcgcggc
     7201 gtttagcgtc atcttcaatc gcttccgcac cgtagcccgc tttttccacc gcctgcacta
     7261 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
     7321 cctgagtgac gcccggtacg ctttgcagcg cattttgtac gcgggtgaca cagctggcgc
     7381 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
     7441 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
     7501 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
     7561 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgctcaacat
     7621 ccgggcgctg ttcaagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccgt
     7681 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
     7741 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
     7801 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatttct
     7861 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcctg aaaatcggca
     7921 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
     7981 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
     8041 acaaaatgcc gattacattc cctttctggc gaatgtacca ggtcaactgg cggcagtggc
     8101 tatcgtgacc tgcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcact
     8161 ggaatccatc tctaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
     8221 ggtacaggac aaaattggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
     8281 agagttgcat ggcggcaaac cgctgtcgcc actggtaaat gctggcgcta ttgccaccac
     8341 cagcctgatt aacgctgaaa atgttgaaca acgctggcag cgaattttac atatccaaca
     8401 gcaactggct ggtgagcagg tagcgctctc tgacgaagtc aaccagtcgg aacaaacaac
     8461 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
     8521 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctct acgctcatca atactgttga
     8581 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgttga cgcataaacg
     8641 cgttcttcag gccgacaacg tgccgtacat tctggccgaa atgatgatgg aagggctgta
     8701 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
     8761 cggtattctg gcggtcgtcc ctggcgtgat gggaattgcc gcgttctcac caccgctgga
     8821 cgaagaaggc aacagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
     8881 ctataacgtg tttaagggct gatcatgatg aacacggaag gtaataacgg taacaaaccc
     8941 ctcggtctat ggaacgtcgt ttccatcggt attggggcaa tggtgggggc ggggatcttc
     9001 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgctttt
     9061 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gtctgggggc gagctatccc
     9121 agcaatggcg gtattatcga cttttttcgt cgcggattag gcaacggcgt cttttcgcta
     9181 gcgctctcgt tgctgtacct gttgacgctg gcggtgagca tcgccatggt tgcccgtgct
     9241 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttattttg
     9301 ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag caaccatgcg
     9361 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatt
     9421 attgccggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
     9481 ggtgcgttct tctcctgtat tgggataacc ttccttgcct atgcgggctt tggcatgatg
     9541 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
     9601 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgta
     9661 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
     9721 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
     9781 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
     9841 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa cattattgtc
     9901 gtggtgttga ttatgctgat gacggcggcg ctaaatttag gctcactcgc cagtgttgcc
     9961 agcgccacct ttttgatttg ctacctggcg gtgtttgtgg tggcgatccg cctgcgtcat
    10021 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    10081 gttggcttta tctacagtct gtggtcccag ggtagccgtg cgttgatatg gattattggc
    10141 tcactcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    10201 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    10261 aaaacgtgct tgaatatctg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    10321 gatgaacatc agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    10381 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    10441 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cggcaggtgg gctttaacct
    10501 ggaagagagc ggcgagctgg tgaatctgtt taacgacccg cagcggcaca gcgccgacgt
    10561 caaacggcgc acgctggaga aggtggcgga gatcgaacga cacattgagg agctgcaatc
    10621 catgcgcgac cagctgctgg cactggcgaa tgcctgccct ggcgatgaca gcgccgactg
    10681 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcagggtgat taagacgaga
    10741 cggctcggat atgtagggtt atcccttcta tcgcaatgac ttcaacatgc gtacctgcac
    10801 cgagatcctc gctggcgctg acaggccatg aactgtcacc gacgcgcata tgaccgcgcc
    10861 cgttgaccag cggagattcc agcacaaaac gtcggccaat cagctgctgc ccgcgctggt
    10921 ttaaatgact atcgctgtgc ttttgttcgc gcacccgccg cgacaaccat ttccaccaca
    10981 gccaggcggc gagcagcgtc aggatggcaa acattacccc ttgccactcc caacccagcg
    11041 gcaccagcca gaccaccagg ccggtaatca ccgctgccac gccgctccac aacaaataac
    11101 catttccgcc cagcatctcg gctgccagca gcaaaccgcc gagactgagc cagaaaatat
    11161 gtggatgaac gaccattaac tccatcatgg ctgagtccgt ttgttggcgc tgtctttcac
    11221 cagctcggca atcccggcaa tcgaccccat caggctgctg gcctctaatg gcatcatcac
    11281 tactttgctg ttactggagg taccgatctg ctgtaacgct tcggtgtatt tctgcgctac
    11341 gaagtagttc accgcctgaa tatcaccgga ggcgatagct tcagacacca ttttggtggc
    11401 gcgggcttct gcttcggcgg aacgttcacg cgcttcagcc tgtaaaaacg ccgactgacg
    11461 ttcgccttcc gctttcagga tttgcgactg tttttcacct tcggctttga ggatttccgc
    11521 ctgacggatc ccttccgctt caagaatgta agcgcgtttg gtacgttccg ctttcatctg
    11581 cgcgttcatt gaagagataa gctctgccgg tgggcgcacg tcgcgaattt caatacgggt
    11641 gactttaatc ccccacgggt tggtggcttc atcgacaata cgcagcaggc gtgagttgat
    11701 gctgtcgcgc tgagagagca tttcgtcaag ttccattgaa cccaacacgg tacggatgtt
    11761 agtcatggtc aggttgatga tcgccagctc cagattgctg acttcataag ccgcgcgcgg
    11821 cgcgtcaatc acctgaataa aacagactgc gtcgatggta acgttggcgt tatctttcga
    11881 gataacttcc tgggaaggga tatcgagcac ttgctccatc atattgatct tgcgaccaat
    11941 gcgatccata aacggcacca ccagactgag ccccggctgt aacgttttgg tatagcgacc
    12001 aaaacgttct accgtccatt gatagccctg cggcacgatt ttgacgcccg cgccgacaat
    12061 gaccagcgcg acaaaaatga gaatcgggat aaagataagc atcggaaaaa cctcctgttg
    12121 taccgtccat aatcagcaaa attgctgctt gattaaacaa attatacctg attactgaaa
    12181 gagagttccc ccttattcct gcgaaggata aactgttttt agtaaaaatc agaaaaaggg
    12241 aacagcgatg caggaaaata gtcctttgct tcagctacaa aacgtaggat atctggcggg
    12301 tgatgcgaag attcttaata acatcaattt ttcgctgcgt gctggcgaat ttaagttaat
    12361 taccggtcct tctggttgtg gcaaaagtac gctgctaaaa atagttgctt cattgatcag
    12421 cccaaccagc ggaacgttac tgtttgaagg tgaggatgtc agcacactaa agccagaaat
    12481 ctaccgccaa caagtctctt actgcgccca gacaccgacg ctgtttggcg atacggtata
    12541 cgataatctg atctttccct ggcagatccg taaccggcag cctgacccag ccatttttct
    12601 cgattttctc gaacgcttcg ccttgccgga cagcattttg acgaaaaata tcgccgagct
    12661 atctggtggt gaaaaacaac gcatctcact gattcgtaac ctgcaattta tgccgaaggt
    12721 tttattgctg gatgaaatca ccagtgcgct ggatgaaagt aataaacata acgttaatga
    12781 gatgatccat cgttatgtgc gcgagcaaaa tattgccgtg ctgtgggtga cacacgataa
    12841 agacgaaatt aatcatgcgg ataaagtgat tacactgcaa ccgcatgccg gagaaatgca
    12901 ggaagcacgc tatgaactcg cataatatta ctaacgaatc attagcactg gcattaatgc
    12961 tggtggtggt ggcaatctta attagccata aagaaaaact ggcgctggag aaagatattc
    13021 tctggagcgt cggacgagcg ataattcagc tgattattgt cggctatgtg ctgaagtata
    13081 ttttcagcgt ggatgatgcc agcctgacat tattgatggt gttatttatc tgctttaatg
    13141 cggcgtggaa cgcgcaaaaa cgcagtaaat atattgctaa agcttttatc tcatcgttta
    13201 ttgccattac ggtcggggcg ggaattaccc tggcggtgct gattctctca gggtcgattg
    13261 aatttatccc gatgcaggtg atccctatcg ccgggatgat tgccggtaac gccatggtag
    13321 cggtggggtt gtgttacaac aatttagggc aacgggtcat tagcgaacag caacagattc
    13381 aggagaaact gagtcttggt gcgacgccga agcaggcttc agcgatattg attcgcgaca
    13441 gtattcgcgc ggctttaatt ccgacggtcg attcagcaaa aacggttggc ttagtgagtt
    13501 taccaggaat gatgtccggg ctgatatttg ccgggattga tccggtgaag gcgattaaat
    13561 atcagattat ggtgaccttt atgctgctct caaccgccag cttgtcgacc attattgcct
    13621 gctatttaac ctatcgtaag ttttataatt cgcgccacca gttggtggtg acgcaattga
    13681 agaagaaatg atgcgatgcc ggatgcgcag catcgcatcc ggcgttgtgg ttcatgtgcc
    13741 gatcaacgga atatcaatac aacaatgcat acagctggcg gcgatacttc gacgccagtg
    13801 catcacccgt acccagcgca gcgaggatct cctggaacgt tttacgcgtc tgaccgtctg
    13861 cggcggtgag atctttacgc agatgcccga acagcaactc cagcgcctct tcattgcgcc
    13921 caacctgatg cagttgcagc gccagttgcg tcgccagtgc ggcatcttct ggattctccg
    13981 ccacctgctg ttgcaactgt tgaatttccg gcgtatcagc cgcctgcttc agcagttcga
    14041 tttgcgccac catcccctgg tagcgggtgt cctgatcctg taacggaatg gttttcagca
    14101 ccgcttctgc atcttcagaa cggttcagcg caatcagcgt ttctgccaac agcaggccga
    14161 tctccccgtt ctgattcgac aactgccagg cgtctttcag caatggcagg gcatcggtga
    14221 aattgccttc ctgcatcagt tgcatcgcct gctgcgcttt cagctcttct tcgcgcggca
    14281 gcactttatc cagcagggcg cggatcgcct cttccggttg cggcccctgg aagccatcta
    14341 ccggttgccc gttctggaac agatacaccg tcggaatcgc gcgcagacca aactgggcgg
    14401 caatcatctg ctccgcatcg cagtccagct tcgccagaat aaattgcccg ttgtactgcg
    14461 ccgcaaggct ttccagaatt ggggttaact gcaaacagtg ctggctacgt tcagaccaaa
    14521 aatagaacag caccggagtg gtcatcgact gttcaagaac ctgttgcagg ttagattcgt
    14581 taatgttgac aatattttct acggacatgg agtcgctctc tgttgtcgat attttctttg
    14641 acatgggggc ttaagcgcgc gcttcaactc acccctgcaa tattttgtcc atcacgcgcc
    14701 ccggcagcag gcgcttaagc accattactg cccaagtcac cagcgttacc ggatagcgca
    14761 tcttcggctt ctcgctaata aaagcatggc gtactttgtc caccaccgct tccggtccca
    14821 acgtaaagcg ggcggcgatg ccgggatttt cgactggttt atcactttgc gtctggttga
    14881 cgttgtcggt gaagcgagta cgaatgggac cgggttcgat caggctgact ttaattccgc
    14941 tgtggcgtag ctccatgcgc agcgcgtctg accatgcctc cagcgcatat ttactggccg
    15001 cgtaagcgcc acgacccggt gtggagatta atcccatcac cgatgatgtc atcacaatac
    15061 gcccttcacc gtgcggtaac atcgcgggta acaggcgcat ggtgagctgg tgtgcgccga
    15121 aaaagttggc ggaaaactgc tgttccatct gcgcgcggct gatggtggaa agggggccat
    15181 acatgccgaa tccggcattg ttaaagatcc catacagaca attatcggtc agggcgatca
    15241 cttcgtcggc tgcgcgatca acactttctg gcgaatccag atcgatcaac acgccggtaa
    15301 atcccatgct gttcatacgc tcaacatcat ccggtttccg gcaacctgcc agcacatgaa
    15361 aaccctggcg ttttaattcg agcgcgcttt ccaggccaat tccactggaa catccggtaa
    15421 ttaagaccga tttttgcata actttacctg tcaggatctc cgttgcttta tgagtcatga
    15481 tttactaaag gctgcaactg cttcgccatc cagtcggcaa taaacggctg ggcgtcgcgg
    15541 ttgggatgaa taccgtcatc ctgcatccat tgtggcttga ggtagacctc ttccataaaa
    15601 aagggcagca gcggaacatc aaactctttg gcgagtttgg ggtaaatggc gctaaaggct
    15661 tcattataac ggcgaccata gtttgcaggc agacgtattt gcattaacaa tggttcagcg
    15721 ttggcggctt tgacatcctg caaaatctgg cgcagcgttt gctcggtttg ctgtggctga
    15781 aaaccacgca aaccgtcatt gccgcccagt tcaaccagca cccaacgcgg ctgatgctgt
    15841 ttcagcagag ccggaaggcg cgccagtcct tgttgcgagg tgtcgccgct gatgctggca
    15901 ttaactaccg acgttttact ctgccactta tcattcaaca aggcaggcca ggccgcgctg
    15961 gcagacattc gatacccggc gctcaggcta tcacccagaa tcaataacgt gtccgctgcg
    16021 gcggcacgga aggttaacag ggccaggaac aggaagggca aatgccagcg gaaaacattg
    16081 ttgaagttca tcatcttaag aagtccgtcg gtcaggggga gcatgaactc tccatcctca
    16141 ccggagttga gctggttgtc aaacgtggcg agaccatcgc actggtgggc gagtcgggat
    16201 cgggtaagtc aaccttgctg gcgatcctcg ccgggcttga tgacggcagc agtggcgaag
    16261 tgagtctggt gggacaaccg ctacataata tggacgaaga agcgcgggca aagttgcgcg
    16321 cgaagcacgt cggctttgtt tttcagtcat ttatgttaat tcctaccctt aacgcgctgg
    16381 aaaacgtcga gcttccggct ctgctgcgcg gtgagagtag cgcggaaagt cgtaacgggg
    16441 cgaaagcgtt gctcgaacag ttagggctgg gtaaacgtct ggatcatctt ccggcacagc
    16501 tttccggcgg tgaacagcaa cgagtggcgc tggcacgagc ctttaatggt cgacctgatg
    16561 tgctgtttgc cgacgaaccc accggcaacc ttgaccgcca gacgggcgat aaaattgccg
    16621 acctgctgtt ttccctcaac cgtgaacatg gcaccacgtt gattatggtg acccacgacc
    16681 tgcaactggc ggcacgctgc gaccgctgct tacggctggt gaacgggcag ttgcaggagg
    16741 aagcatgatt gcacgttggt tctggcgcga atggcgttcg ccgtcgctat taattgtctg
    16801 gctggcgcta agcctggcgg tggcctgcgt gctggcgctg ggcaatatca gcgatcgcat
    16861 ggagaagggc ttaagccagc aaagccgtga gtttatggcg ggcgatcggg cgttgcgcag
    16921 ttcacgcgaa gtgccgcaag cgtggctgga ggaagcgcaa aagcgcggcc tgaaagtcgg
    16981 caagcagctg actttcgcca caatgacctt tgcaggcgac acaccgcagc tggcgaacgt
    17041 caaagcggtg gatgatatct acccgatgta tggcgatctg caaactaatc cccctggcct
    17101 gaaaccgcag gcgggcagcg tattgctggc cccacgcctg atggcactgc ttaacctgaa
    17161 aacgggcgac accattgacg tgggcgatgc caccttgcgg attgccggag aagtgattca
    17221 ggaaccggat tccggtttta accccttcca gatggctccg cgtctgatga tgaatctggc
    17281 ggatgtcgat aaaaccggag ccgtgcagcc ggggagtcgg gtcacctggc gttataaatt
    17341 cggcggcaac gagaaccagc tcgacggcta tgataaatgg ttgttacctc agcttaaacc
    17401 cgaacaacgc tggtacggtc tggaacagga cgaaggcgcg ctggggcgat cgatggaacg
    17461 ctcgcaacag ttcctgctgc tttcggcgct tctgaccttg ctgctggcag tggcagcggt
    17521 ggcggtagcg atgaatcatt actgtcgcag tcgctacgat ctggtagcga tcctcaaaac
    17581 gctgggggca gggcgagcgc aactgcgtaa gctaatcgtc ggtcagtggt tgatggtgct
    17641 gacgctttca gccgttaccg gtggggccat aggcctgttg ttcgaaaacg tgttgatggt
    17701 gctgctcaag cccgttctgc ctgctgcact accgccagcc agcctctggc cgtggctgtg
    17761 ggcgcttggc accatgacgg tcatctcgct gctggtgggg ctacgaccat accgcttgtt
    17821 gctggcaacg cagcctttac gcgtattacg taatgatgtg gtagcgaacg tctggccgct
    17881 gaagttttat ctgccgattg tcagtgtggt ggttgtgctg ctgctcgccg gattaatggg
    17941 tggcagcatg ctgctttggg cggtgctggc gggcgcggta gtactggctt tgctgtgcgg
    18001 tgtgctgggc tggatgcagc tgaatgtact tcgccgcatg acgctgaaat cgctacctct
    18061 gcgcctggcg gttagccgcc tgttacgtca gccgtggtca acgttaagcc agctttcggc
    18121 attttcgctc tcctttatgc tgctggcgct gttgctggta ttgcgtggcg atctgctcga
    18181 ccgctggcag cagcagctac cgccagaaag cccgaactac tttttaatta acatcgccac
    18241 agaacaggta gcaccgctaa aagcgttcct cgcggaacat catatagtcc cggaatcatt
    18301 ttatccggta gtgcgggcgc ggctgacggc gattaacgat aagccgacag aaggtaatga
    18361 agatgaggcg cttaatcgcg aactcaatct tacctggcaa aatacgcggc ccgatcataa
    18421 tccgattgtc gccggtaact ggccgccaaa agccgatgaa gtgtcgatgg aagaggggct
    18481 ggcgaaacgc ttaaacgttg ccctcggtga taccgtgact tttatgggcg atacccagga
    18541 gttccgcgct aaagtgacca gcctgcgcaa agtggactgg gaaagtctgc ggcctaattt
    18601 ctattttatt ttcccggaag gggcattaga cgggcaaccg cagagctggc ttaccagttt
    18661 ccgctgggag aatggcaacg gcatgttgac gcaactcaac cgccagttcc cgaccattag
    18721 cctgttagat attggcgcga ttttaaaaca ggtcggtcag gtgctggagc aggtaagtcg
    18781 ggcgctggag gtgatggtgg tgctggtcac cgcctgcggt atgttgctgt tgctggcaca
    18841 ggtgcaggtg ggaatgcgtc agcgtcatca ggagctggtg gtgtggcgca cactcggtgc
    18901 ggggaaaaaa ctgctgcgta ccacgttgtg gtgtgagttc gccatgcttg ggtttgtttc
    18961 tggcctggta gccgcaattg gtgcggaaac ggcgctggca gtgttgcagg cgaaagtgtt
    19021 tgatttcccg tgggagccag actggcgatt gtggattgtt ctgccgtgca gcggtgcact
    19081 gctgctgtcg cttttcggcg gctggctggg tgcgcgactg gttaagggta aggcgctgtt
    19141 caggcagttt gcggggtgat gacaggtgtt agattgcctg atgcgctacg cttatcaggc
    19201 ctacgaagag cgtgcaatat attgattttg caagattgtg taggccggat aaggcgttta
    19261 cgccgcatct ggcatgaaca gtgtgcattt tggcaataaa tatgaaacca actgtcatgt
    19321 cggtttttta tatctgtatt ctggtgcaac cattctgttc tcacgacaat cgccgtttca
    19381 gaataccgcc catcactgcg tcttaaccca ttccgccact tccgcccact caccgcgaaa
    19441 gacaactttt tccgcttttt tctcaagctg atagcgatac atcgggtcgt aatattcttt
    19501 aagtaacggc accagccagg ccagatgacc gtcggtgctg tcggtggtga gttgcgttgt
    19561 cagcgccgta tccagctggg cagcgagttc gttatagcgc tgtaacccca gccgacgctt
    19621 aatagccgaa agtccgtgat gcaggtattc gcaatattcc tgccagccct gttcgtcgcc
    19681 gtacgcgtgg gtaaaatcat gatgcatacg caagaaatac tcttcgttca ggcgctcaag
    19741 acggatctca aacggatctt ctaccaccgc aatcgccgcc tgagtcattc gctcgcgcag
    19801 gcattccggc aggtgattcg aaccgatcat ccggctttcg tcttccagca cccacaggcg
    19861 caaatcctga cgggcgtcgg tttttagcat ttcggcagcc agcaggtttt caaaactcgc
    19921 ctggctaagt tgtggttgta aggtgcgacc aaacgccgaa ccgcgatgac gcgctaaccc
    19981 ttccagatca acaccgttcg gctgttgctg cactaacagc gttttaccgc tgccggtaca
    20041 accgccaatc agcactatcg gtttttgtgc cagttcaata gtcgcctgaa tcgcggcttg
    20101 gcgcagtgcc ttataaccgc cttccaccag cggataatca atccccgctg catacaacca
    20161 gctttgcaca atatgtgagc gctgaccgcc acgggcgcag cagagaatac cttgcggatt
    20221 ttgcaggcac gctgcccgcc aggcgtccat gcgctgctga cgaatttcac ccgccaccag
    20281 tttatgcccc agcgccagcg ccgcgtctga gccttgctgt ttataacaag tgccaacggc
    20341 ggcgcgttca tcgttattca ttaacggcag gttgatagcg gcgggcattg cgccttgctc
    20401 aaactcgata ggggcgcgaa catcaataat gggcgtatca gcaatcagca gggcacgata
    20461 gtcctgttcc gtgtgtctct cttgcatagt taaaagtgaa cctcaaatca gcttgcgcgc
    20521 tattttacgc gccaacgcgc aaggaaactt gatttttaac tgcgtgggtt gccgaaaatt
    20581 tctaaaaatc cgctgatttc cggcctgcgc tgggtaaaca aggtcacaat atcttctacc
    20641 gctttgccgc tgccaaattt gcgccatgcc agactcaatg gcgaaggagg gcgcatcgtt
    20701 gggattaccc ggctgaccag ttgttgatta tcgatcattg actggcaaag tgattttggc
    20761 aaaaaaccaa tgccaacacc cgccagatgg gcggcgattt tcgtttccat atcgggaaca
    20821 ataatctctt tttgccccgg caatcgccag gcgacgcgtt tggttaaggt tcgggcgctg
    20881 tcttcaatat tgaccgccgg aaagcgccgc aactgcgctt ctgttagcgg ctcttcaacg
    20941 ttcgccagcg gatgatccgc cgccatgaca aagcgccatt gcaccgatcc taagggatca
    21001 agactaaagg tatttgccag cgcctcagtt cccgtgaccc cgatagccag cgaaaaacct
    21061 tcgtacaata gcgagtccca gacgcccata tagatttgtc gggagatgtg aaactgggta
    21121 aaggggtaac gctcattcag ccacgccagc aactgggcga cggcctgggg gttatagagc
    21181 aggttgttga tgacaatatt cacctagcgt tccacgccat cattcacctg ttgcagctcg
    21241 ctgggcatac tttccagcca gctcagccag tctctggcct gggaaagtag atgctcgcca
    21301 gccgctgtca acgtcacgct gcgagtcgtg cggaaaaaca gcgccactcc ggtattctct
    21361 tccagaagtt taatgcgata actgatcgtc gccgtggttt tacataatcg ttctgccgct
    21421 tttgaaaaac ttcctgtttc agcaaccgca atgaaagtcc gcaaggtttc tggatcgaac
    21481 atcttcaggt atcccctttt aaatccgcaa gttgcgtgat tttcttatcc tctgatttat
    21541 cagtattttt acatgataac cctgttcaat ttgtggacta aatctagttt tggaaaaata
    21601 ttccaacttt tgtattgatg ttgttctctt aaggttttag at
//
LOCUS       DAZPEP010000058        21437 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZPEP010000058 DAZPEP010000000
VERSION     DAZPEP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.058"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            130..906
                     /gene="exuR"
                     /locus_tag="ACHUT9_002171"
     CDS             130..906
                     /gene="exuR"
                     /locus_tag="ACHUT9_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFR1348642.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1146..1229
                     /gene="yqiM"
                     /locus_tag="ACHUT9_002172"
     CDS             1146..1229
                     /gene="yqiM"
                     /locus_tag="ACHUT9_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFR1348643.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1251..1913
                     /gene="yqjA"
                     /locus_tag="ACHUT9_002173"
     CDS             1251..1913
                     /gene="yqjA"
                     /locus_tag="ACHUT9_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFR1348644.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1917..2300
                     /gene="mzrA"
                     /locus_tag="ACHUT9_002174"
     CDS             1917..2300
                     /gene="mzrA"
                     /locus_tag="ACHUT9_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFR1348645.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2447..2815
                     /gene="yqjC"
                     /locus_tag="ACHUT9_002175"
     CDS             2447..2815
                     /gene="yqjC"
                     /locus_tag="ACHUT9_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFR1348646.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2853..3158
                     /locus_tag="ACHUT9_002176"
     CDS             2853..3158
                     /locus_tag="ACHUT9_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFR1348647.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3161..3565
                     /locus_tag="ACHUT9_002177"
     CDS             3161..3565
                     /locus_tag="ACHUT9_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFR1348648.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3555..3854
                     /locus_tag="ACHUT9_002178"
     CDS             3555..3854
                     /locus_tag="ACHUT9_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFR1348649.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4040..4432
                     /locus_tag="ACHUT9_002179"
     CDS             4040..4432
                     /locus_tag="ACHUT9_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFR1348650.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4502..5487
                     /gene="yqjG"
                     /locus_tag="ACHUT9_002180"
                     /pseudo
     CDS             4502..5487
                     /gene="yqjG"
                     /locus_tag="ACHUT9_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
     gene            5780..6145
                     /locus_tag="ACHUT9_002181"
     CDS             5780..6145
                     /locus_tag="ACHUT9_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1348651.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPSENRFGPDPKLEP"
     gene            6387..6743
                     /locus_tag="ACHUT9_002182"
     CDS             6387..6743
                     /locus_tag="ACHUT9_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFR1348652.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(6794..7686)
                     /gene="yhaJ"
                     /locus_tag="ACHUT9_002183"
                     /pseudo
     CDS             complement(6794..7686)
                     /gene="yhaJ"
                     /locus_tag="ACHUT9_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            7791..8492
                     /locus_tag="ACHUT9_002184"
     CDS             7791..8492
                     /locus_tag="ACHUT9_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFR1348653.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            8515..8679
                     /gene="yhaL"
                     /locus_tag="ACHUT9_002185"
     CDS             8515..8679
                     /gene="yhaL"
                     /locus_tag="ACHUT9_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFR1348654.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8813..10123)
                     /gene="cyuA"
                     /locus_tag="ACHUT9_002186"
     CDS             complement(8813..10123)
                     /gene="cyuA"
                     /locus_tag="ACHUT9_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFR1348655.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(10151..11482)
                     /locus_tag="ACHUT9_002187"
     CDS             complement(10151..11482)
                     /locus_tag="ACHUT9_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1348656.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGAFYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(11757..13121)
                     /gene="tdcG"
                     /locus_tag="ACHUT9_002188"
     CDS             complement(11757..13121)
                     /gene="tdcG"
                     /locus_tag="ACHUT9_002188"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1348657.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(13193..13582)
                     /locus_tag="ACHUT9_002189"
     CDS             complement(13193..13582)
                     /locus_tag="ACHUT9_002189"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFR1348658.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13596..15890)
                     /gene="tdcE"
                     /locus_tag="ACHUT9_002190"
     CDS             complement(13596..15890)
                     /gene="tdcE"
                     /locus_tag="ACHUT9_002190"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFR1348659.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15924..17132)
                     /gene="tdcD"
                     /locus_tag="ACHUT9_002191"
     CDS             complement(15924..17132)
                     /gene="tdcD"
                     /locus_tag="ACHUT9_002191"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFR1348660.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(17158..18489)
                     /gene="tdcC"
                     /locus_tag="ACHUT9_002192"
     CDS             complement(17158..18489)
                     /gene="tdcC"
                     /locus_tag="ACHUT9_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFR1348661.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18511..19500)
                     /gene="tdcB"
                     /locus_tag="ACHUT9_002193"
     CDS             complement(18511..19500)
                     /gene="tdcB"
                     /locus_tag="ACHUT9_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFR1348662.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19599..20537)
                     /gene="tdcA"
                     /locus_tag="ACHUT9_002194"
     CDS             complement(19599..20537)
                     /gene="tdcA"
                     /locus_tag="ACHUT9_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFR1348663.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20852..21070
                     /gene="tdcR"
                     /locus_tag="ACHUT9_002195"
     CDS             20852..21070
                     /gene="tdcR"
                     /locus_tag="ACHUT9_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFR1348664.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
     gene            21326..>21437
                     /locus_tag="ACHUT9_002196"
     CDS             21326..>21437
                     /locus_tag="ACHUT9_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348665.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKF"
BASE COUNT         5587 a         5523 c         5040 g         5287 t
ORIGIN      
        1 gaatttataa atattcacaa tattaattca atacattaat tcaatcacca aatttatata
       61 tgaccaatgg catacccatg aataaagtgg tatgataact tcgtccgcat tccactccgg
      121 gagacgcata tggaaatcac tgaaccacgc cgtttgtatc aacaacttgc cgctgacctg
      181 aaagagcgca ttgaacaggg cgtctatctg gtgggtgata aactgcctgc cgaacgcttt
      241 attgccgatg aaaagaacgt cagccgaacg gtagttcgtg aagccatcat catgctggaa
      301 gttgaaggct atgtggaggt gcgtaaaggt tcaggtattc atgtggtttc caaccagccg
      361 cgccatcagc aggcggctga caataatatg gaattcgcca attacggtcc gtttgagttg
      421 cttcaggctc gccagctcat cgaaagtaat attgccgagt tcgcggcaac tcaggtaacg
      481 aaacaagaca tcatgaaact gatggccatt caggaacagg cgcgcggcga acaatgcttc
      541 cgtgattccg agtgggattt acagttccac attcaggtag ccctggcgac gcagaactcc
      601 gccctggcgg ccatcgttga aaaaatgtgg acccagcgta gccataaccc gtactggaaa
      661 aaactacacg aacacattga ttcccgtacc gttgataact ggtgtgatga ccacgatcaa
      721 attctcaagg cgttaattcg caaagatccc catgctgcta agctggcaat gtggcagcat
      781 ctggaaaaca ccaaaatcat gttatttaac gaaaccagcg acgatttcga gttcaatgcc
      841 gaccgctatc tattcgccga aaacccggta gtgcatctcg atacggccac cagtggcagt
      901 aaatgaaact cctgtccgac aggcgctgcg acgcgcctgt tcgcttcgtc ggtaagcgaa
      961 aagtataaag tgtcagcctg tgtaaatcct ctcgccaccc tcccctgcat tcagcaaaat
     1021 cagactccac ggacatgtaa ttttgataac gaacaacgtt gacctttgtt acaattagat
     1081 tcaatttgaa tttatgtttt tgaatgcttt cttatctcac gatttaacag ggaatagttc
     1141 aggctgtgtt gatgtatcaa acccgcagaa cataccaaaa cagcaataac attgcggtag
     1201 tgcatctttt aaaaccagcg tggcgttaac cgattcacca ggaataatga atggaacttt
     1261 tgacccaatt gctgcaagcc ctgtgggcgc aggattttga aaccctggcc aatccatcga
     1321 tgattggcat gttgtatttt gtcttgtttg taattttgtt ccttgaaaac ggcttgcttc
     1381 cagcggcctt tttaccgggc gacagtttac tggtattggt cggcgtgttg attgcgaaag
     1441 gcgcgatggg ttacccgcaa acgattctgc tgctgaccgt tgccgccagc ctcggctgct
     1501 gggtcagcta tattcagggg cgatggctgg gcaatacccg caccgtacaa aactggctat
     1561 ctcatttacc cgcgcattat catcaacgcg cacaccatct ttttcataaa cacggtttat
     1621 cggcgctgtt aattggtcgc tttattgcgt ttgtcagaac actgctgccg acgattgccg
     1681 ggttatcagg gctgaataac gcgcgctttc agtttttcaa ctggatgagc ggtctgctgt
     1741 gggtattgat cctgacaact ctgggttaca tgctcggcaa aacgccgata tttttaaagt
     1801 acgaggacca gctgatgtca tgcctgatgc tgctcccggt ggtgctgctg gtgtttggcc
     1861 tggcaggttc tctggtcgtg ttatggaaaa agaaatatgg aaatcggggg taagggatgc
     1921 aaatacctcg catgtcgctt cgccagctag cctggtccgg cactgtttta cttctggtcg
     1981 gcacgctgtt gctggcctgg tccgcggttc gccagcaaga gtctacgctg gcgattcgtg
     2041 ccgttcatca aggcacaacg atgccagacg gtttttcgat ctggcatcac cttgacgctc
     2101 atggcattcc tttcaaaagt atcaccccca aaaacgacac tctgttaatt acatttgact
     2161 ccagcgacca gagcgccgcc gcaaaagcgg tcctcgacag aacattgccc catggctaca
     2221 tcattgcgca gcaggacaat aacagtcagg ctatgcagtg gctgacccgg ttacgggata
     2281 actctcatcg cttcggataa cttcctgtta tccgaaacat atcactcact ttggtgattt
     2341 caccgtaact gtctatgatt aatgaggcgg tggttaacac taccgcttcg ttgtctggac
     2401 aatcgttcct ttgtaatagg tccgaatcac aatggaaggt tcaagaatga aataccgcat
     2461 cgctttagct gtttctctct ttgctcttag tgccggtagt tatgccacta ccctgtgtca
     2521 ggaaaaggag caaaatatcc ttaaggagat cagctatgcc gaaaaacacc aaaaccagaa
     2581 tcgtattgac ggtctgaata aagccctgag tgaagtccgg gccaactgtt cagatagcca
     2641 gctgcgtgcc gatcatcaca agaaaatcgc aaagcagaaa gatgaggtgg cggaacgcca
     2701 gcaagattta gccgaggcga agcaaaaagg cgatgccgat aagattgcca aacgcgaacg
     2761 gaaactggca gaagcgcagg aagagctgaa aaagctggaa gcgcgcgact actaactcac
     2821 aatagtcact acttactcac ctggagaaaa ctatgtcgaa agaacacact acggaacatc
     2881 tgcgtgctga gttgaaatcc ctttccgata cgctggaaga ggtgcttagc tcatctggcg
     2941 agaagtcgaa agaagagttg agtaagattc gtagcaaagc ggagcaggca ctgaaacaga
     3001 gccgttatcg cctgggtgaa accggtgatg ccatcgccaa acaaacccgt gtcgcggcgg
     3061 cgcgtgctga tgagtatgtg cgtgaaaatc cgtggaccgg cgtgggaatt ggcgctgcaa
     3121 tcggtgtagt gctcggcgtt ctgctgtcgc gtcgttaatt atggcggaca ctcatcacgc
     3181 gcaagggccc ggtaaaagcg ttctgggcat cgggcagcga attgtttcca tcatggttga
     3241 aatggtggaa acacgtctgc ggctggcggt ggtggagctg gaagaggaaa aagctaatct
     3301 ctttcaactt ttactgatgc tgggcctgac gatgcttttc gctgcatttg gtcttatgag
     3361 cctgatggtg ctaattattt gggcggttga tccgcaatat cgcctgaatg cgatgattgc
     3421 caccaccgtg gtgttgctgc tactggcact gattggcggt atctggacgt tacgtaaatc
     3481 gcgtaaatcc accttgctgc gccatacacg ccatgagctg gcaaacgatc ggcaactgct
     3541 cgaggaggag tcccgtgagc agtaaagtcg aacgtgaacg acgtaaggcg caactgctta
     3601 gccagatcca gcaacaacgg ctggatcttt ccgccagtcg tcgtgaatgg ctggaggcaa
     3661 caggcgctta cgatcgtcgc tggaatatgc tgctaagtct gcgctcctgg gcgctggttg
     3721 gcagtagcgt gatggcgatc tggacgattc gccatcctaa tatgctggtc cgctgggcca
     3781 gacgcggttt tggcgtatgg agcgcctggc gtctggttaa aacgaccctc aagcagcaac
     3841 agcttcgcgg ttaacttccc tctggccgga gcaattccgg ccttatccct caaatttttt
     3901 gaagattttt gacagttttc cttgctaaca atcatcattc accacgttta tgattctctc
     3961 catcgacagc aacgacgcta ataccgcgcc cttgcacaaa aaaacaatca gcagcctgag
     4021 tggcgcagtg gagagtatga tgaaaaaatt agaagatgtt ggtgtactgg tagcgcgcat
     4081 tttaatgccg attctgttta ttaccgcagg ctggggaaaa atcaccgctt actctggtac
     4141 ccaacaatat atggaagcaa tgggtgtccc gggttttatg ctgccgctgg tgattctgct
     4201 tgagttcggt ggtggtctgg caatcctgtt cggtttcctg actcgcacca cagccctgtt
     4261 tactgcgggc tttacgctgc tgacggcatt tttatttcac agcaactttg ctgaaggcgt
     4321 caactcgctg atgttcatga aaaacctgac aatttctggc ggattcctgc tgctggcaat
     4381 taccggtccg ggcgcgtata gcatcgaccg cctgctgaat aaaaagtggt aagcacgcgc
     4441 tatacttaac gataaaaaac gaggaggaag ctcctcgttt ttgctattgg aggagagaaa
     4501 aatgggtcaa ctgattgacg gcgtctggca tgacacctgg tacgatacca aatctaccgg
     4561 cggtaaattt caacgttcag cttccgcatt tcgtaactgg ctcactgccg atggcgcacc
     4621 tggccccact ggcacaggcg gttttatcgc agagaaagat cgttatcatc tctatatttc
     4681 actcgcctgc ccgtgggcgc accgcacgct gatcatgcgc aaactcaaag gactggaacc
     4741 gtttatttcc gtttccgtag tgaacccgct gatgctggaa aacggctgga cctttgatga
     4801 cagttttccg ggagcaaccg gagatacgct ctatcaacat gaatttctgt atcagcttta
     4861 tctccacgcc gatccacact acagcggacg agttactgtt cccgtactgt gggacaaaaa
     4921 gaaccacacc atcgtcagca acgaatcaac agaaatcatc cgcatgttca ataccgcgtt
     4981 tgatgcgctg ggcgcgaaag cgggtgatta ctacccacca gccctgcaaa cgaaaattga
     5041 cgaacttaac ggctggattt atgacaccgt taacaacggc gtgtataaag ctggttttgc
     5101 caccagccag caagcctacg acgaggcggt ggcgaaagtg tttgaatcgc tggcgcgact
     5161 ggagcagatt ttaggccagc atcgttacct gaccggcaac cagctaaccg aagccgatat
     5221 tcgcctgtgg actacgctgg tgcgttttga tccagtgtat gtgacccact tcaagtgtga
     5281 taagcaccgc atcagcgatt acctgaatct gtatggcttc ctgcgcgata tctaccagat
     5341 gccggggatc gccgaaactg tcaatttcga tcacatccgt aatcattact tccgcagcca
     5401 taagaccatc aaccctacgg ggattatttc aattggtccg tggcaggatc tcgatgaacc
     5461 gcaggacgag atgttcgctt cggttaaaaa aaggcacctt acgaagtgcc tctgataatt
     5521 agctaagtgc atgttgttca tatcggatgc ggcgtgaacg ccttatcaaa cctacaaaac
     5581 atttatattc aatatgttgc aatgaccgcg caggccagat aagcgtagcg catctggcga
     5641 ttttgcgctt gtcatcagtc ttagcgcgcc ttcacagcac tttattaatt ccttgaacga
     5701 atatttactg ccatttcatt cacgtctatt cttaatttgc tgctttaaaa acaagtgatt
     5761 gagcaaaatt gaggcaaaaa tggactggta tctgaaagta ataaaaaatt atgtcggttt
     5821 ccggggtcgt gcgcggcgca aagagtactg gatgtttatt ctggtcaaca tcatctttac
     5881 gttcgtgctg gggctgctgg ataaaatgtt aggctggcaa cgcgctggtg gcgaaggcat
     5941 cctgacgaca atctacggta ttctggtgtt tttaccgtgg tgggcagttc agttccgccg
     6001 cctgcacgac accgaccgct cggcgtggtg ggcactgcta ttcttaatcc cgtttatcgg
     6061 ctggcttatt atcatcgtct ttaactgcca ggcgggtacg ccaagcgaaa accgctttgg
     6121 acctgatcca aaactggaac cttaaaacaa atctgctcgc cggatgtctg actcatccgg
     6181 cttttatcat catgttgatg aatgaataat ttactaatgt ttatttaaaa tatttcaatg
     6241 tcgttatata aattacctat aaaaaataac catgtattta tcttaaatat caaaaccttt
     6301 ataacatccg aaaaagaaag atacgaatag catttacaca atacccggaa tagactttcg
     6361 tcacttttaa ttaaagggat gtttttatgc agtggtattt atccgtatta aaaaattatg
     6421 ttggtttctc tggtcgcgca cgtcgtaaag agtactggat gtttactctg attaacgcca
     6481 tcgtcggcgc tattatcaat gtcattcaat tgattttagg tctggagctt ccatatctgt
     6541 ctatgcttta tttgctggca accttccttc ccgtccttgc gcttgctatc cgtcgcttac
     6601 acgataccga ccgttcaggt gcctgggcct tgctcttttt tgttccgttt attggctggc
     6661 tcgtgcttct ggtttttttc tgcacagaag gtacttctgg cagcaatcgt tacggaaacg
     6721 atccgaagtt tggttcaaat taatcttaga attggggcga tatttcgccc ctttttatta
     6781 acaataataa atattatttt ccgctaaaaa gtttgggaat ttcccgcaga caccaggatt
     6841 tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctca ctggtcgatt
     6901 ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc ggatacggca
     6961 tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc gtgctcaccg
     7021 ttaagcgcgg ctgtttgtcc agcagttgta cggtcaacac cgggcgctca cgagcggtat
     7081 ccgctaccgc aattccacga tatttcacgc gcgtcacttc agataacggt tccggctcct
     7141 gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag agcttgcgcg
     7201 agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacaata tccgcccgcc
     7261 cctgctccag tcgttcctac gcccccgcca gcacttcggt gatgattgcc agttgggtat
     7321 tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt accagcgctt
     7381 cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag ggcttccgca tcggtagtca
     7441 gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc ccgacattgg
     7501 tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct tcttccagtt
     7561 tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgcc gccgccgcaa
     7621 aactgccccg gcgatcgcat ccataacccg tagtgcttcc agcgttaatg ccctttcttt
     7681 ggccatttcg ttctcattca ggaaatttga acataccggg cagaatatct ggctaacaat
     7741 gtagcgtcca gccccttacc ataaaaggaa gtaaagagag gtcaagaatt atgattacta
     7801 cccgaactgc caggcagtgt ggacaagcag actacggatg gttgcaggcc cggtatactt
     7861 tttcctttgg acactacttc gacccgaaat tgttaggcta tgcctccctg cgtgtgctta
     7921 accaggaagt gctggcccca ggtgccgcct ttcagccgcg aacctatccc aaagtcgata
     7981 ttttaaatgt gattctggat ggagaagcag agtatcgcga cagcgaaggc aatcatgttc
     8041 aggccagcgc cggtgaggcg ttgctgctct ctacccagcc gggtgtcagc tacagcgaac
     8101 acaatctcag caaagacaaa ccgttaacac gaatgcagct ttggctggac gcctgcccgc
     8161 agcgagagaa tccgctgatt caaaaactgg cgcttaatat gggcaagcag caattaatcg
     8221 cctcgccaga gggtacgatg ggaagcctgc aattacgcca gcaagtgtgg ctgcaccata
     8281 tcgtgctcga caaaggcgaa agtgcgaatt tccagttgca tgggccacgc gcgtatttgc
     8341 aatcgattca cgggaaattt catgcgctta cgcatcatga agagaaagcg gcgctgacct
     8401 gcggtgatgg ggcgtttatt cgtgacgagg ctaacattac gctggttgcc gattccccac
     8461 tgcgcgcttt gctgatagat ttgcctgtct agttgttttt acagggagat gatgatgagt
     8521 aaaaaatcgg ccaaaaagcg ccagccagtg aagcccgtgg tggcgaaaga acctgctcgc
     8581 accgccaaaa attttggcta tgaagagatg ttgagcgagc tggaagccat cgtcgcggat
     8641 gctgaaacgc gtttagccga ggatgaagct accgcgtaaa gacaggttgc cggatgcggc
     8701 gaaaacgcct tatccggcct acaaaatcca acaacattaa cgaattaaac aacccgtagg
     8761 ccggataagg tgcgccagca tcgcatccgg cacaatactc aaaacctggc gtttatctgg
     8821 ccttgctcgc cataatctcg ataatctgcc gatccgtttg ctgcatcgaa tggcttgcta
     8881 acgcacacag gttggcaatc gactgctcaa catcatgcgc cacgatccct tcattgccgg
     8941 tcacggcggt atcatccagc gccattaaca ccgctttcca cgcagccgaa gcactggtcg
     9001 aaaccttcat cgcgcagctg ttcgacgcac catcgcaaat catgccgctg acatcgccga
     9061 tcatactgct gatcgccatc gagatagttt cataacgccc atccaccagc catgccatcc
     9121 cggcggcggc ccccattgct gcggtcgttg cggcacaaag tgcagacaaa cgcggtaact
     9181 ggttatggat gtaaattgcg ctcaaatgag aaagcatcag cgcacgcgcc agtcgttcat
     9241 catccgctcc gaagtgttct gctacaacca ccacaggcat tgttgcggtg atcccctggt
     9301 tacccgagcc ggagttactc atagccggaa gcgtagcgcc gcccatacgc gcatcggatg
     9361 ccgcgctggt acgaatcaca atggatgaag agagatcttt cgccagcaaa ccgcgctcgc
     9421 actgtttttc cagcgtcgcg ccaatatgca gcccccactt accgctcaaa ccttcctgcg
     9481 ataacgcaca atttagcttc gcggaatcga gaataaagcg gatcgccgca aacgggactt
     9541 cattgacgaa cttcaggatc tcagccagcg tcgttctgga aagcaccgtc agcggagact
     9601 cttgctcgcc ctctgccaca cacgcctgct gggtaaacac cacaccattg tgcgtctcaa
     9661 tatgcacaat gttggtatgc ccgccgacga tggtgacaca cgcccacttc tcaccgttcc
     9721 agactttggc gcgtgagaag aggatttcat tgcaaggttc ctggatctta acggagactt
     9781 tccccgccgc cagcagtgct ttggcatcgg caattgcctg cgcagttgcg tctttcagca
     9841 cttccagccc ggcgttggca tttccaccta acgcccccag cgccgccgca atcggcagcc
     9901 ccaccattcc cgtgccggga acggtgacgc ccagaccgtt cttcatcaga tttggcgaaa
     9961 cccaggcttc tacacgttca accggacctt ccagttctgc cgcagcaacc gccgccgcca
    10021 gcgccagtga aatcggttca gtacatccca gcgccggttt tacttcctcc tgaacggcga
    10081 ggatgtaacg ctgccataac ggatttaaag tcgaatcaaa catattaaaa accttaaaat
    10141 ttcaggtaaa tcaagaaaat gccaggaacg gagaaacaca aagcaacaaa ccagtgacga
    10201 taatcaggta cagagacatc cctttgtatt tgtgcaatgc cggtactttg taaaccagcc
    10261 acgccgggat caggcaccct accatgccga aaatcgggct acagatagag gtgaagctca
    10321 acaccggtgc gttcagtacg atagcgctcc aggccagcaa aatggcgaaa atcatgatgc
    10381 cgcgctgaac aagattttcg ttaatcttct cggcaggcat cttgcgacgc aggatgttca
    10441 ttacgatccc ttgcgttgct tcgcgaaagc ctaaatagac gccaaagaat gcagtcatga
    10501 cagcaaagat attgagaatg acgctgacca ctttcaccca cgctgcgccg tcaccgctaa
    10561 taaactgtgc ggcaatcgcc agcgcggaaa tattctgctc gtaggctttt accgcttcgt
    10621 catgccccat cgccagcgtg aacgacacgg cgtagaaaaa gacggtgaca aacaaaatgc
    10681 caaacgcgat attcattgcc cgcaatgctt tatgccgcgc cacttcaata gatttttccc
    10741 gcgagcgata agagatcacc atcgggctta acgtctggat aaacaaaatc gacgtcaggg
    10801 taaacggcag cgtaataatg gcgtttttca ccagcagccc cagcggcggt agcgaaccga
    10861 cgttatacag atgccacatt cccaccatcg acacgcccag cgccgcgacc accagcagct
    10921 tggtcagcac catgccggtc gaaattttga ataacaattt ctcgccgcgt gaagagatcg
    10981 ccaccagaat gcaaatcagt accagaccat aaaagggact gtctgacagc aacccttccg
    11041 tcacgccgaa ggtatgcaga taggaagcac tatcgttggt gatggcggtg gaatagacga
    11101 acatccaaat caccagcatt acgaaataga acgcacctaa caggatgccc cagtttttac
    11161 ctaaataacc gctaatgaca ctcgggtaat ctttacattc tggtgattct gccagcgtat
    11221 taataaacaa ccgctgaaac agatacattg ccgggtaacc aatcaccgat gagagcaaaa
    11281 atacccacaa tcccatcaaa ccgacctgca ccgggagaaa aacaatcccc gcgccaatcg
    11341 ccatcccgat actcataatc acccagccgg tgtcagtact gtcgaatttg atcgcttctc
    11401 gccactcgct ctcactcatt ccagcacgac ccgccggggt cgaagcgtct gcaatgacgc
    11461 ctttattcga tgcaatttcc ataatttctc gctcaatatt ttgtagggct tattattttt
    11521 ttccgagccg catcaaggcg atatgcggtc tacgtttttg caggcgagtt attagaataa
    11581 agaaatgata cgcgcgaggt cagagaaaat cttcacaatc aagactttga aataacggag
    11641 atggataaga attttctact aaattaatcg caagagaata ttcattctct atttgcggca
    11701 gatcacaaaa aaaaggtgca catttgtgca cccaaggatg aaagctgaca gcaatgtcag
    11761 ccgcagacca ctttaatggc cagtcctccg cgtgatgttt cgcggtattt atcgttcata
    11821 tctttgccgg tttcatacat cgtctcgatc actttatcga gtgaaacacg cggtgccgag
    11881 gtgcggcgca tcgccatcca cgcggcgttt actgctttca cggcattaat ggcattacgt
    11941 tcaatgcacg ggatttgtac ctgtccggca accggatcgc aggtcagccc aaggttatgc
    12001 tccatcgcga tttccgccgc attgcatacc tgcgccggac tgccgcccag tagttcagtt
    12061 aaccctgccg ccgccattga acaggccacg ccaatctccc cctgacagcc gacctccgcg
    12121 ccagagatgg aggcgttcat tttatacagc gcgccaatag cccccgctgc cagaaaatag
    12181 cgggcaattg accgctcgtt taccggacga cggaacttat cgtaataagc cagcactgcc
    12241 ggaataatgc cgcacgcacc gttagtcggt gccgttacca cgcgcccgcc agctgcgttt
    12301 tcttcactaa ccgccagcgc gtacatgttg atccagtcga tgacattcat cggatcgtta
    12361 gagatgttat cgctagaaac cagctgacga cgcagcgcta cggcacgacg cggcacattg
    12421 agcggaccag gcagcacgcc ttcagtgttc atcccgcgtt caataccgtc atgcatcact
    12481 tgccagatac gggcaaatcc tgcgtcaatt tccgctttgc tgcgcagcgc cagctcgttg
    12541 tgcatcatca ggccagatat agacaggccg ttgtaatcac acattttcag cagttcacct
    12601 gctgagtgga aatcgtaagg tacggacgtt tcgacatcgt gcgacaggcc gaagtgttct
    12661 tcttcgacaa taaacccgcc gccgacagag taataggttt tacttaatag cgcttcctgt
    12721 cccttccagg cagtgatccg cattccgttc tcatggcgag gcaacatttc gggatggaag
    12781 ataatgttct ttgctacagg aaaatcaaca atatgcgcac cagatgccac tggcagccgc
    12841 ccgctgcgcg ttactaactc tataaatgca gggatctcat caatgacaac atcctgcgga
    12901 ctgtttcctg ccagtcccat gataatggcg acatccgtgg catggccttt gcccgtcagt
    12961 gacaacgacc catacagatc gaccacaata tggctcgtcg cggttaataa gccgctactt
    13021 tccagccgat caataaaact ttttccggca ttcattggcc ccacggtatg cgaactggag
    13081 ggaccaatcc caattttgaa aatatcgaat gcactaatca tatccacacc ctcggattgc
    13141 cgttcagtga agtggagcgg aacgaccttg cgaccgtccc gctcacgagg ctttacgcac
    13201 tacgtactgc gatagcttca atttccagct tcacatcttt cggcaaacgc gcaacctgca
    13261 cacagctccg ggtcgggtag gtcgcctgat gctcatcgaa gaactgctta tagacttcat
    13321 tgatggtggc aaaatcattc agatcggtga tgaacacggt catcttgatg atatcgccca
    13381 cgctcagccc ggcagcaacc acgatcgctt tgacgttttc gaggcttaaa cgcgcctgat
    13441 cttgcacatc agccgggatc tcaccggtct gcgggcaaac cggaatttgc ccggaggtga
    13501 agaccatgct gcctaaatca acgccctgaa cataagggcc gattgcgcct ggggcacgtt
    13561 gcgtttcgat aatctttttc atacatcctc cggcgtcaga gcgcctgggt aaaagtacgt
    13621 gaaataacat cctgttgctg ttcacgggtc agtgcgttga agcgcacggc gtagccagag
    13681 acacggattg tcaggttagg atatttttcc gggtgctcga tggcatccag cagcatttcc
    13741 cgattcatta cgttgacgtt gaggtgttga ccgccttcga catccgcttc gtggtggaaa
    13801 tacccatcca gcaggccgac aaggttggtt ttacgtactg gatcttcttt gcccagcgcc
    13861 gcaggaacga ttgagaaggt gtacgagatc ccatctttgg cgtaggtgaa cggcagtttc
    13921 gccaccgacg tcaatgaggc cacggcacct ttgcggtcac gaccatgcat cgggttagcg
    13981 cccggcgcga acggtgttcc ggcgcgacga ccgtccggcg tattaccggt tttctggccg
    14041 tacaccacgt tagaagtgat agtcagaatc gactgggtag ggacggcgtt gcgataggtt
    14101 ggcagcgctt taattttctt cataaagcgt tcaaccaggt cgcaggcaat gctgtctacg
    14161 cgctcgtcgt tgttgccgta ctgcggatat tcaccgtcga tttcaaagtc caccgccagg
    14221 ccgttttcgt cacggattgg tttcacgcgg gcatatttga tggcagacag ggagtccgtc
    14281 gccaccgaca ggcccgcgat gccgcatgcc atagtgcgat agacatcacg atcgtgcagc
    14341 gccatcagcg aagcttcgta gctgtacttg tcgtgcatgt agtgaatgat attcagcgcg
    14401 ctgatgtact gcaccgccag ccagtccatg aagtgatcga ggctgtccat cactttgtcg
    14461 taatccagca cgtcgtccat cagcggtgct gttttcggcc cgacctgaat cttcagcttc
    14521 tcgtccaccc cgccgttaat tgcgtacagc agtgttttcg ccaggttagc gcgtgcacca
    14581 aagaactgca tttgcttacc aatcaccatt gggctgacgc aacaagcaat tgcgtaatcg
    14641 tcgctgttga agtcagtacg catcagatcg tcgttttcat actgcaagga agaggtgacg
    14701 atcgacacct gcgcggcata ttttttgaag gcaatcggta attcttccga ccaaagaatg
    14761 gtcaggttag gttccggtgc cggccccata gtgtgcaggg tgtgcaaata gcggaaggag
    14821 tttttggtca ccagcgtacg accgtccagc cccatcccgc cgatcacttc cgtcgcccag
    14881 attgggtcgc cggagaacag cgaatcaaat tccggcgtac gcaagaagcg caccatacgg
    14941 atcttcataa tgaagtgatc gatcagttcc tgtgcctgct gctcattgag tacgcctgct
    15001 ttaaagtcgc gctcaatgta gatatcgagg aacgacgccg tgcgccccag cgacatcgcg
    15061 ccgccgttct gtgatttcac tgccgccaga taagcgaagt agagccactg caccgcttcc
    15121 tgcgcattct gcgccgggcg agagatatca aagccatatt tcgccgccat ttcctgaatc
    15181 tgcaacagcg catgacgatg ctctgccagc tcctcacgca gacggatggt ggcttccaga
    15241 tcttcgcctt tttccagacg ggactggaga tcggcaaatt gcagttcgcg ttcacgcacc
    15301 agataactga tgccatacag cgctacgcgg cgatagtcac cgataatgcg cccacggcca
    15361 tagccatctg gtaaaccggt cagcacgcca gatttacggc agcgcagcat atccggtgag
    15421 taaacatcaa atacgccctg gttatgggtt ttacgcagat cggtaaacag atattcaaat
    15481 tcactgtcca tttcacggcc ataggcgtgg aatgaacttt taatcatatt aatgccaccg
    15541 aacggatgca gcgcgcgctt taatggcgca tccgtttgca gaccaacaat tttttccagc
    15601 ggctggttaa tatatcccgc atcatgagcg gtaattgtgg tggcaatatt ggtatcgaaa
    15661 tcaaccggcg cgtgggttgc gttttcgata cggatgcctt ccattacttt ttcccacaat
    15721 tccgtggtgg caggcgtcgc ttcggcgagg aaagattcat cgccttcata cggtgtatag
    15781 ttatgttgaa taaaatcgcg gacattaatt tcgtttttcc agtccgtacc tttaaagcca
    15841 agccatgcgt cggcgtacag cttatcgctg gtatcaatat ctatcttcat gaaaaataat
    15901 ctctctacaa tacttcaact aaattatgca aattctgcgg gcgcgttaac tttgcctaaa
    15961 tgaatggcat ccaaagcaat cattttttct tcgttagtcg gaataacggc acaaatgaca
    16021 cgcgcatttt cactggaaac aattcgctct ccaaaagagt tagagcgatt attcatttct
    16081 gtatcaatca ctacgcctaa tacagccaaa tgttccatga ccagacgacg aattaaactt
    16141 gaattctctc ctattccgcc ggtgaatata attccatcca ggcgacgtaa tgaagctgcg
    16201 tgtccggcaa tatgacgggc aattcggtga acaaaggttt taattgccag ttgcgcgcgt
    16261 tcgtgacctt catgccaggc tttttccaga acacgtaaat ccgaagaaag accggaaata
    16321 cctaataatc ccgactcttt attcactacg cgttccaggt cacccaggct ctggttggtt
    16381 tggctggcga cccaggacat cgcaccaaag tcgacatcgc cactgcgggt tcccatcatc
    16441 aagccttcca gcggcgtcat tcccattgaa gtatcaacac tctgaccgtt gcgaaccgcg
    16501 cagattgacg cgccattgcc aagatgcgcc acaaccaggc cggagtcatc ttccgccaga
    16561 ttcagcagag aatgtgcgcg ctgggaaaca tagcggtgcg acgtgccgtg gaaaccataa
    16621 cggcgtacac ctaactcttc ataatatttc cacggcaggc cgtataaata agcttccgga
    16681 gccatcgtct ggtggaaact ggtatcaaat accgccacct gagttacgcc cggaaataat
    16741 tgctgcgccg attcaatacc acttaaattg gcgtaattat gcaggggtgc cagtggagaa
    16801 acgcgacgga tattatcaat gacttcatcg gtaataatgg cggactcggt aaaaatactg
    16861 ccgccgtgag cgatgcggtg gccaattaag gccacactgt catttaaatt ccgtttttcc
    16921 agttcaaatg caattgcctt caatgcacct tcgtagctgt ggtgagccag cggtgctggc
    16981 tctcccccat ttacggataa gaatgcattt tccgagttaa taccgtcggc aatacctgac
    17041 attaatactt cacagtcgct ggcatcgagc acggaaaact taatcgaaga cgaaccacag
    17101 ttaataacca aaacaaccgg aaattcattc atctcttttc tcatcctgag ttacggatta
    17161 aaacagtttg tatacgatgt tcaggatggt cagcagacca atcacggtaa caaacacgtt
    17221 atccagacga ccacggtatt tcgccagaga cggcgcttta cggatggcat acatcggcaa
    17281 caggcacagc agggatgcga taatcggtgc gcccatggct tcaatcaggt cgaggatgtt
    17341 cgggttggcg taggcaacaa cccaggtgga gcccatgatg aagatcatgc tgatagtgtt
    17401 cagtttaccc agcgacactt tagttttgtc gcctttataa ccaaacttca ggaccaggcc
    17461 attcagacct tccagcgttc ccagatagtg accgaagaaa gatttgaaga tagccacgag
    17521 tgcgatgatg gaagccgcat attccagtgt aatcgcgaac gttgttttgg taccggtcat
    17581 ggacgcaaag tggttagcca gataagaaag cactggaata ttctgcgctt tggcttccgc
    17641 catgttggcc ggagacagcg taaataggca gctaaaggca aagaacatca ccactgcaac
    17701 catcagcatg ctggcacgag aaatgatttg ggaacattta cgttcggtga agtcgcgacc
    17761 gaagtctttc tcatactctt cacgtttaga aaccacgaag gaagagacga ttggcgagaa
    17821 attaaaggag aaaaccatga tggaaatccc cagccagacg gtgatcagga taccgtcatg
    17881 accggttaac gacagcgaac cgaggtcaac ctggtcgata actgcagagt tccagtaagg
    17941 gatcagcgac agagaaatca gcaccaggct ggcgataaac ggccatacca ggtagctcat
    18001 cactttaacc atcagatcct taccaaacca gatgacgaaa gccatcagca gcagcaggaa
    18061 cagcgccaca aagccgcgat tcagcggtgc aaagccgagc tggttttccc agaacgtcat
    18121 aaaggtattg gtaatagtaa cgccataaat ccacagcagt gggcaaatcg cgaagaagta
    18181 caggaacgtg ataaccacgc cgccagtttt accaaaatgc tcttccaccg tttccgtaat
    18241 gttgccggaa gggttagagc cagaaagaca tagacgcgcc agcgcccggt ggcaataaaa
    18301 tgcgatgggg tatgccaata ccaacatcag aagaatcggg atcagtccgc caaaacctgc
    18361 gcggataggg aagaacagca ccccggcgcc gattgccgta ccaaacaagc ctaacgtcca
    18421 tgtggtatct gatttacgcc aggacgattg ttttgtctgg ctggatacaa tgctatctga
    18481 agtactcata tcctatcctc aacgaattaa ttaagcgtca acgaaaccgg tgatttgaga
    18541 gacgcgagaa agatcgatat tgccgccgga aataatactg acggtttttc tgttttgaat
    18601 atattggtct aatttaccgc ttaataatgc agcacatgcc agagcgcctg cgccttcggt
    18661 gacgacttta ttgcgctgaa ttaaggcaat catactgttt ctgatttcgt cttcgctgac
    18721 cagcacgatg tcatcgacta attcacgaac gatttcgtaa gttaaattac ccgggcggga
    18781 gacatcacaa ccatccgcca gggtgccggt agttcggtgc gtggttattt ctccggagtg
    18841 gaaagaagcc gccatgccgt gaacgttttc agactgtacg ccaataacac gaatggtcgg
    18901 gttaatagat ttaattgcca ccgcaatacc agcaattaaa ccgccaccac caattggcac
    18961 aatcacgtta tcgacatcat agagatcttc cataatttcc agaccaatcg ttccctggcc
    19021 agcaatcact ttcggatcat cgtaaggtgg gataaaaata cggccttcca tttcgacaat
    19081 ttcgctcact ttagcgatag tgtcgttgaa gttatcacca tgcagaacga cttctgcgga
    19141 gtagtcgcac gttgccgcta ctttggattt tggcgcacct tttggcatca ccactttacc
    19201 gtcgataccc agcatcgcgc aggagagcga aaccccttgc gcatggttgc ccgcagaaca
    19261 ggccaccacg cctttgcgtt tttccgcatc ggtcagtgaa cttaatttat taaatgcgcc
    19321 acgaatttta aatgaacccg tacgctgcat attttcaaac ttcaggaata tttcaccttt
    19381 gcaacgttca ctaaaatagt tggagcgagg catgcctgtt ttataaattc gcccagccag
    19441 tcgttgtttc gcttcaataa tatcatcaat agcaatcggc agatcgtatg taatatgcat
    19501 tataaaacct cttcgcctga attaaatatg taggtaaccg taaccgacac ctgcaaaaca
    19561 gacaggtgga ttatttatgt ttaaataaaa acaaataact aaccaacttc tattaattgc
    19621 cttcgtctac acccattata agatgaatac tctttggcta attccaccaa aaccgatgct
    19681 gcttttttaa tacgataatt tttcgaccat accgcggcat attgtgccac aggtaatgtt
    19741 tcttcaaccg gaatagtaat aaattgatta gaaccaaaag gtgacgtcat atcacaagga
    19801 attacagtta agaaatcagc attgagaaca agattataaa ttgtcacgac tgagtcggtt
    19861 ttaacgatgt tttcaatact gatgccattt ctttgtaacg tagtaagcag ttcgctgtag
    19921 taccccatat tagtttgtgg caacacccac tgttcgttct tcaacgactc cagcgtggtg
    19981 gtgccggtgc atgttcggga cttactggct accagcacaa actcggactc gaacagcggc
    20041 tcaacatgta aatcctgaag cttcatttct gcacttaacg taccaatcgc aaaatccagt
    20101 cgaccgtcgc ggattgccgg taagaacgaa gacagttgcg cttcatacat agaaacctgc
    20161 gctttcggga acacctcttt gaacttgttg atcatccctg acataaaagt aaaaccaatc
    20221 aatgaaggaa aaccaaatga gacttccacc accgcctcag aagacatacc gcttatctca
    20281 ttaaccatat ttttcatttc acgggtaatg gattcggaac gggagagtaa caattgaccg
    20341 gcaggtgtta atgttacacc ggtatttttc cgcaccacta attccacacc aaaataatct
    20401 tcaatatcgt taatgatttt actgacggcc ggttgagtta accctaattc ttttgcagcc
    20461 gagccgatag agccacttct aatgacttcc tgaaagacta ccaggtgctg cgttttcgga
    20521 agaagaatag tgctcataac gacctacgtt aattacctca ttgacggcat gaagtgtatc
    20581 aaaatgaaat gaacaggata tgtgcgacca ctcacaaatt aactttcaat actttccagg
    20641 gtatcgtcat tacaattaaa tacctttaaa tatcaacaag ttaaagcata aaaatcagca
    20701 taaaccctga tttttgtcaa aaaaaatgac ggggataacc atattttatg gcgataacat
    20761 cattcgttat gtggttaata caaaaaaggg gctgagacca tatttcaagc aattaccgga
    20821 taattaccag gcaaaatttg agttaaattt aatgagcaag ttctcaaatt ttattataaa
    20881 taaaccattt tcagcgataa atactgcggc acgtcacatt ttttcacgct atttgttgga
    20941 gaacaaacat ttattttatc aatattttaa aatttcgaat acaggtattg atcatctcga
    21001 acaattgatt aacgtcaact ttttctcttc tgacaggacg tcattttgtg aatgcaatcg
    21061 ttttccataa attcttctcc cctcataggc gacgaatagc attttgtgtt gaggatcaca
    21121 aaacgaataa ttgctgatcg ccgcgataag gtcagacaaa gacaacaagg gaaattttca
    21181 cagagctttt gatcgtcgta ggccacagaa tgttgcttcg tttactgtaa cgccgggtaa
    21241 atgagcgttt tttgatagtg cgaaagaacc ctgcgaggaa aataagcatc tattattgtt
    21301 tgtcatgacg acaaggcagg catcaatgaa aggcttccca attgcacata tttttcaccc
    21361 ttcaatcccg ccaatgcacg cagtggttaa caatcacaat agaaatattg attattggac
    21421 ggtaaaaaga aagtttg
//
LOCUS       DAZPEP010000059        21003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZPEP010000059 DAZPEP010000000
VERSION     DAZPEP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.059"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..170)
                     /locus_tag="ACHUT9_002197"
     CDS             complement(<1..170)
                     /locus_tag="ACHUT9_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0758 domain-containing protein"
                     /protein_id="HFR1348666.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUT9_002198"
     CDS             342..1562
                     /gene="coaBC"
                     /locus_tag="ACHUT9_002198"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFR1348667.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1540..1998
                     /gene="dut"
                     /locus_tag="ACHUT9_002199"
     CDS             1540..1998
                     /gene="dut"
                     /locus_tag="ACHUT9_002199"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFR1348668.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUT9_002200"
     CDS             2105..2701
                     /gene="slmA"
                     /locus_tag="ACHUT9_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFR1348669.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUT9_002201"
     CDS             complement(2738..3379)
                     /gene="pyrE"
                     /locus_tag="ACHUT9_002201"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFR1348670.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUT9_002202"
     CDS             complement(3445..4161)
                     /gene="rph"
                     /locus_tag="ACHUT9_002202"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFR1348671.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4288..5151
                     /locus_tag="ACHUT9_002203"
     CDS             4288..5151
                     /locus_tag="ACHUT9_002203"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFR1348672.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5373..6197
                     /gene="dinD"
                     /locus_tag="ACHUT9_002204"
     CDS             5373..6197
                     /gene="dinD"
                     /locus_tag="ACHUT9_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFR1348673.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6489..7106
                     /locus_tag="ACHUT9_002205"
     CDS             6489..7106
                     /locus_tag="ACHUT9_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFR1348674.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHUT9_002206"
     CDS             complement(7103..8785)
                     /gene="ligB"
                     /locus_tag="ACHUT9_002206"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFR1348675.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9043..9666
                     /gene="gmk"
                     /locus_tag="ACHUT9_002207"
     CDS             9043..9666
                     /gene="gmk"
                     /locus_tag="ACHUT9_002207"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFR1348676.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHUT9_002208"
     CDS             9721..9996
                     /gene="rpoZ"
                     /locus_tag="ACHUT9_002208"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFR1348677.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10015..12123
                     /gene="spoT"
                     /locus_tag="ACHUT9_002209"
     CDS             10015..12123
                     /gene="spoT"
                     /locus_tag="ACHUT9_002209"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFR1348678.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12130..12819
                     /gene="trmH"
                     /locus_tag="ACHUT9_002210"
     CDS             12130..12819
                     /gene="trmH"
                     /locus_tag="ACHUT9_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFR1348679.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12825..14906
                     /gene="recG"
                     /locus_tag="ACHUT9_002211"
     CDS             12825..14906
                     /gene="recG"
                     /locus_tag="ACHUT9_002211"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFR1348680.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHUT9_002212"
     CDS             complement(15075..16280)
                     /gene="gltS"
                     /locus_tag="ACHUT9_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFR1348681.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16560..17951
                     /gene="xanP"
                     /locus_tag="ACHUT9_002213"
     CDS             16560..17951
                     /gene="xanP"
                     /locus_tag="ACHUT9_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFR1348682.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18072..19781
                     /locus_tag="ACHUT9_002214"
     CDS             18072..19781
                     /locus_tag="ACHUT9_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFR1348683.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKHQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHUT9_002215"
     CDS             complement(19833..>21003)
                     /gene="yicI"
                     /locus_tag="ACHUT9_002215"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1348684.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5718 c         5385 g         4817 t
ORIGIN      
        1 ccataaagag agccgaaatt ctccagcatc tcttttgcca gggttaatac atctttaccg
       61 cgcgttccgg tacgcagaaa tagcgccagc agctcgacat ccgttaaggc gctaatacca
      121 aacttcagca ttttttcgcg cggcatcaac agctgtgcat tgtttttcac cttcacctcc
      181 tttgtggtgc tcgcatcctg tcatagccgt ttcgttaaat cgacggccag ttttcaatcc
      241 tggaaagcgc ctcgcaaagt gaatctcagg tgaaggcacg acaacaaaag tattgtgata
      301 aaatcgccaa cttctggtgt caccctacag gaaaaatcat catgagcctg gccggtaaaa
      361 aaatcgttct cggcgttagc ggcggtattg ctgcctataa aacccctgaa ctggtgcgtc
      421 gtttgcgcga tcgcggggcc gacgtccgcg tagccatgac cgaagcggca aaagccttta
      481 tcaccccact tagcttgcag gcggtttctg gttatcccgt ttccgacagt ctgctggacc
      541 cggcagccga agccgctatg ggccatattg agctgggtaa atgggctgat ttagtgattc
      601 tcgcccctgc cacggcagat ttgattgccc gtgttgctgc cggaatggcg aatgacctgg
      661 tatcgacgat ttgtctggct acacctgcgc ctgtagccgt gctccccgcc atgaaccagc
      721 agatgtaccg tgccgctgcc acgcagcata atttagaggt gcttgcttcc cgtggtttgc
      781 tcatctgggg gccagacagt ggcagtcagg cttgtggtga tatcggtcct gggcgaatgc
      841 tcgatccgtt aaccattgtg gatatggcgg tagcgcattt ttcgcccgtc aacgacctga
      901 aacatctgaa cattatgatt accgccggcc cgacgcgtga accgctcgat ccggtgcgtt
      961 atatctctaa tcacagctcc ggcaagatgg gttttgctat cgccgccgcc gctgcccgtc
     1021 gtggcgcgaa cgtcacgctg gtatcaggcc cggtttcgct accgacgcca ccgtttgtta
     1081 aacgtgttga tgtgatgacc gcgctggaaa tggaagtcgc cgtgaatgct tctgtacagc
     1141 agcaaaatat ttttatcggc tgcgccgccg tggcggatta tcgcgcagct accgtggccc
     1201 cagagaaaat caaaaagcag gccacgcagg gtgatgaatt aacaataaaa atggttaaaa
     1261 accccgatat cgtcgcaggc gttgccgcac taaaagacca tcgaccctac gtcgttggat
     1321 ttgccgccga aacaaataat gtggaagaat acgcccggca aaaacgtatc cgtaaaaacc
     1381 ttgatctgat ctgcgcgaac gatgtttccc agccaactca aggatttaac agcgacaaca
     1441 acgcattaca ccttttctgg caggacggag ataaagtctt accgcttgag cgcaaagagc
     1501 tccttggcca attattactc gacgagatcg tgacccgtta tgatgaaaaa aatcgacgtt
     1561 aagattctgg acccgcgcgt tgggaaggaa tttccgctcc cgacttatgc cacctctggc
     1621 tctgccggac ttgacctgcg tgcctgtctc gacgacgccg tagaactggc tccgggtgac
     1681 actacgctgg ttccgaccgg gctggcgatt catattgccg atccttcact ggcggcaatg
     1741 atgctgccgc gctccggatt gggacataag cacggtatcg tgcttggtaa cctggtagga
     1801 ttgatcgatt ctgactatca gggccagttg atgatttccg tgtggaaccg tggtcaggac
     1861 agcttcacca ttcaacctgg cgaacgcatc gcccagatga tttttgttcc ggtagtacag
     1921 gctgaattta atctggtgga agatttcgac gccaccgacc gcggtgaagg cggctttggt
     1981 cactctggtc gtcagtaaca catacgcatc cgaataacgt cataacatag ccgcaaacat
     2041 ttcgtttgcg gtcatagcgt gggtgccgcc tggcaagtgc ttattttcag gggtattttg
     2101 taacatggca gaaaaacaaa ctgcgaaaag gaaccgtcgc gaggaaatac ttcagtctct
     2161 ggcgctgatg ctggaatcca gcgatggaag ccaacgtatc acgacggcaa aactggccgc
     2221 ctctgtcggc gtttccgaag cggcactgta tcgccacttc cccagtaaga cccgcatgtt
     2281 cgatagtctg attgagttta tcgaagatag cctgattact cgcatcaacc tgattctgaa
     2341 agatgagaaa gacaccacag cgcgcctgcg tctgattgtg ttgctgcttc tcggttttgg
     2401 tgagcgtaat cctggcctga cccgcatcct cactggtcat gcgctaatgt ttgaacagga
     2461 tcgcctgcaa gggcgcatca accagctgtt cgagcgtatt gaagcgcagc tgcgccaggt
     2521 attgcgtgaa aagagaatgc gtgagggtga aggttacacc accgatgaaa ccctgctggc
     2581 aagccagatc ctggccttct gtgaaggtat gctgtcacgt tttgtccgca gcgaatttaa
     2641 ataccgcccg acggatgatt ttgacgcccg ctggccgcta attgccgcgc agttgcagta
     2701 atatgacgcc ggatgacttt tcatccggcg agtttcttta aacgccaaac tcttcgcgat
     2761 aggccttaac cgccgccaga tgttccgcca tttccggctt ctcttccagg taagcaatca
     2821 ggtctttcag ggtgatgata gagatcactt tgcagttgta atcacgctca acttcctgaa
     2881 tcgccgaaat ctcgctgcgc ccccgttcct gacgatcgag cgaaatcaac acgccagcaa
     2941 gcgtcgcgcc attggcctga ataatttcca tcgactcacg aatcgccgtt ccggcggtga
     3001 tcacatcatc taccagcatt acgcgtcctt gtaacgcgct accaaccaga ttgccgcctt
     3061 caccgtggtt ttttgcctct ttgcggttaa agcagtacgg caggtccagg tcatgatgct
     3121 ccgccagcgc cacggcggtt gtggtggcaa tcgggatccc tttgtaagca gggccaaaca
     3181 gcagatcgaa ctcaatgccg gaatccacca atgcttcagc gtaaaaacgg cctaacagtg
     3241 ccaggtcgcg cccggtatta aacagcccgg cgttgaagaa ataggggctt ttacgcccgg
     3301 atttcagcgt aaactcgcca aactttaaca cctgcttgct aagcgcaaat tcaataaact
     3361 ggcgctgata tggtttcatg ccttcgctcc tcatcttact tttctacaga caaaaaaaag
     3421 gcgactcatc agtcgcctta aaaatcagtt tgccagcgcc gccttctgcg tcgctacaat
     3481 ggattcgatt ccccctcggg ccagagccaa caaggtgagt aattcttcgt gggtgaacgg
     3541 ttcgccttct gccgtcccct gcacttcaat gatgcgcccg tcttcggtca tcactacgtt
     3601 catgtcggtc tctgcggcag agtcttcaac gtattccaga tcgcaaaccg cttcgccgtt
     3661 cacaatcccg acagaaaccg cggctaccat ccctttcatc ggattggttt tcagcttacc
     3721 gttttccacc agcttctgta gcgcatctgc cagcgccacg caggcacccg taatcgaagc
     3781 ggtacgagtg ccaccatcag cctgaagcac gtcgcagtcc agcgtaatgg tgaactcacc
     3841 cagcgctttc aaatctactg ccgcgcgaag cgcacgggcg atcagacgct ggatttccat
     3901 tgtgcgtcca ccctgcttac ctttcgccgc ttcacgagcg ttacgggtgt gggtagaacg
     3961 cggcagcata ccgtactctg cggtgatcca gccctggccc tgacctttca ggaagcgcgg
     4021 cacgccttct tcaatagagg cggtacacaa cactttggta tcgccaaatt cgaccagcac
     4081 cgagccttct gcatgttttg tatagttacg agtcagggta acgggacgca cctgattatt
     4141 gctacggcct gctggacgca tattgaaatc tccggcttga aacaaatgtg gctgcgcatt
     4201 atacggactt ccggaggtta ttcctatcct gacaaggcat cgatggctat aatccttcca
     4261 cctctccttt tataaacagg aacgtctatg atccgcagta tgaccgccta cgcccggcgt
     4321 gaaatcaagg gtgaatgggg gagcgcaacc tgggaaatgc gctcggtaaa ccagcgttat
     4381 ctggaaactt actttcgtct gccggagcag ttccgtagcc ttgaacctgt cgttcgcgag
     4441 cgtattcgtt ctcgcctgac gcgcggtaaa gtggaatgta ccctgcgcta tgagccagat
     4501 gttagcgcgc aaggtgagct gatcctcaac gaaaaactgg ctaaacagct ggtaactgcc
     4561 gcgaactggg taaaaatgca gagtgacgaa ggggaaatca acccggttga tattctgcgc
     4621 tggccgggcg taatggcagc ccaggagcag gatctcgacg cgattgccgc tgaaattctc
     4681 gcggcgttgg atggtaccct ggacgacttt atcgtcgcac gtgaaaccga aggtcaggcg
     4741 ctgaaagcgt tgatcgagca gcgtctggaa ggcgtcaccg ccgaagtggt caaagtccgc
     4801 gcccatatgc cggaaatcct gcaatggcag cgtgagcgtc tggtcgcgaa gctggaagat
     4861 gctcaagtgc aactggaaaa caaccgcctg gagcaggagt tggttctgct ggcacaacga
     4921 attgatgttg ccgaagaact ggaccgcctg gaagcgcatg tcaaagagac ctacaacatt
     4981 ctgaagaaaa aagaagccgt tggtcgtcgt ctggacttta tgatgcaaga gttcaaccgc
     5041 gagtcgaaca ctcttgcgtc gaaatctatc aatgccgaag tgacgaattc tgccatcgag
     5101 ctgaaagtgt tgattgagca gatgcgcgag cagattcaga atattgagta actcgtaaca
     5161 ctaattctta cctttcagcc ttcacaaatg ccgcccactc aaacagagcg gcatttttct
     5221 tccccgcaac attcaattct gttttgcgta cctgctccag attttgcgat gtttttttgc
     5281 gcagcgcagt gagaacgtga gatactcaca actgtatata aatacagtta tagacttact
     5341 ttctttgcaa tcgatatcac atggagtggg caatgaacga acatcatcaa ccttttgaag
     5401 aaatacggca ttatggcact gaagggcaag aattttggtc agcgagagaa ttggcaccgt
     5461 tactggatta tcgggactgg cgcaattttc aaaaggtact ggcccgcgcg actcaagcgt
     5521 gcgaagccag caatcaagct gcatctgacc atttcgttga aaccaccaaa atggttgtat
     5581 taggttcagg ggctcaacga gaacttgaag atgttcatct ctctcgctat gcctgttacc
     5641 tggtagtaca aaacggcgac cctgcgaaac cggttattgc ggcagggcaa acttattttg
     5701 ctatccagac ccgacggcag gagctggctg acgatgaagc tttcaggcaa cttcgtgaag
     5761 acgaaaagcg tctttttctg agaaatgagt tgaaagaaca taacaaacaa ttagttgagg
     5821 cagcgcagca agctggggta gcgacagcta tcgatttcgc catatttcag aatcatggtt
     5881 accgggggct gtatggtgga ttagatcaga aagccatcca tcagcggaag gggctgaaaa
     5941 agagtcagaa gatcctggat catatgggtt caacagaact ggcggctaat ctctttcgag
     6001 ctacccaaac agaagaaaaa ctcaagcggg atggcgtaaa ttcaaaacag caagctaata
     6061 ccacacactt tgacgtgggt agcaaggtga ggcaaaccat tcaggaactt ggcggaacca
     6121 tgcctgagga gttgccgacc ccgcaggtca gcatcaatca gttggaaaac agtgtaaaaa
     6181 ttacagagaa gaaatagcag aaactaaaaa ctctttttct taattgagat acccgatgcg
     6241 taagccaaag tccaggtgca gtgaacacaa tggctaaaca ttgcaccttt cttttccacc
     6301 cctcaatttt aacctatttt ttcttatgca ttttctcaga caagaagtca gaagaatgca
     6361 tctacactac agaaaatagc gatttcacat aactacaagt tatcaatttc ctctcacctt
     6421 aaaaaatctc aatcgtgaca atacgcacaa atcgctaccc tgccagacag atttttaggg
     6481 agagaaccat gctgttacac attttgtatt tggttggcat tactgccgaa gccatgacgg
     6541 gggcgctggc ggccggacga cggcgcatgg atacatttgg cgtaattatt attgcgaccg
     6601 ccaccgcaat tggcggaggg tcagtgcgcg atattctgct gggccactat ccgctcggct
     6661 gggtcaaaca cccggaatat gtgattatcg tcgctaccgc cgcagtactc accactatcg
     6721 ttgctcctgt aatgccttac ctgcgcaaag tgtttctggt gctcgatgcg ctcggactgg
     6781 tggtcttttc tatcatcggc gcacaggttg cactggatat ggggcacggc ccaattattg
     6841 ccgttgtcgc ggcggtaacc accggcgtgt ttggcggcgt tttgcgcgat atgttctgta
     6901 aacgcatccc actggtattc cagaaagagt tgtatgccgg tgtctcattt gcctccgccg
     6961 tgctgtacat cgcgctgcaa cactatgttt ctaaccatga tgtggtgatt atctccaccc
     7021 tggtattcgg cttttttgcc cgcttactgg cgctacgtct taagctggga ttaccggtct
     7081 tttactacag ccacgaaggt cactaaggtt caaaacctgt gatctgctgg gcagccagcc
     7141 aactgcccag cttcttgatt tgcgcatttt ccttccattc aataacctgt ctggcgcgcc
     7201 cagatccagt ccccggcagc cgctgccaga actgctccgt gctaaataaa agttgcgacc
     7261 aggaccgttc atcactggca ttaagcgccg cccgggttaa cggtattccc attgccatca
     7321 cccagcgagt aaaaggctgc ttacgagcca gattaaactg atgccataac tgcgcacttt
     7381 tacttttcgc gatccccggc gtgttctgta attgctctgg cgttaataaa agccaggaaa
     7441 agatatgctc aaagcgatga gtctgatgca gcgcgcgcca gccggcctca ccaatgccat
     7501 ccagcccaag aacctgtttt gaccccagcc agactaagcg tgaaatgaac tgttcctgac
     7561 aaacatcaga agcaaagtag caggtcaacg agttaaagcg gttttctggt ggtgtcggtt
     7621 ttgtacgttc tgcaccgcgc cagaccacat catcaatgcg aggaataccc tgaccggcaa
     7681 ggctgacgag gatctgatca ccaggcgcaa tatcccactc ctgccagcgc ctgacggaac
     7741 caatattcac ccgctggact tttttatcat ccagcatgac aggtgcgagt gacgcaacca
     7801 ccgatatttt accgctctta cccaccgcaa actgaatcgc cttcacttcg gcaacctgag
     7861 ctacaggttg atatttccag gccaccaacc attctgcctg gcctggtagc caatggcggg
     7921 attcgggttc cttagccgct cgcacaacca cgccatcggt gacgaagggt aatttcgctt
     7981 tccaccactc attgcgtacg cgcgcaactt catcagcatt tttaaccgca cgggtatacc
     8041 tctgcgttag agtaaaacct gcggtagcca gctcctttaa acgatcggtc attaactgcg
     8101 gtccatccgg ccatgcccag acaaaaacag ccagtgagtt tagcgtgtcg ctattcccct
     8161 ggcgcatcat caggccagca acttttgcgc gggcatttat tccccccatt tgttgttgga
     8221 tatgcccctc gcgctggaga aatatttccc cctgaagcgt actgttggct aaaggcccgc
     8281 taacggtttg cggcacagcg gaaattaagc taactttctg cgtccagtcc tcgcctttca
     8341 ggccattacc gcgactgatt gctttgttca gtttcccgtc ccgataaacc agggttaccg
     8401 caacgccatc aacttttggc tgcacccaaa gatcgctacg ttctcgcatc cacagactta
     8461 atgcgttttt atccaccatt ttgcgtacac ccgtatgggc aaccggatgc ataaccgcac
     8521 catttaaagg cggcatcatc acgtcgcggg gctcgctccc aaagcagcgt tgccactgcg
     8581 taagacgggc gcttaactga tcgtaaacac cgtcttccac ctcacttttc ccttccttcc
     8641 agtagtcatc gtcccactgt tttatttgct gttgcaggcg ggaaatttct tcctgtgctc
     8701 tggctggcga ccaggccgga cagaccgccc acacagatga ttgccagcac aagatactta
     8761 ttaatatcgc catccatact ttcatcatca cctccgctgt agatagtcag gcagatatac
     8821 aacgtgatga aagcaaagcc gagtggcaaa aacggagtct gcgaggacgc ttcctgaaaa
     8881 tcgtctttat tgcaatgaat gacaggcaaa tgcggaagca gctacgcaaa acgcaacaac
     8941 tttgcgcaaa aagtgtgagc aagggctacg tcacatggcc gcgccgtgta taataagctc
     9001 gtatgtaggc tttatttcgc taatcacata cgaaagatac tcatggctca aggcacgctt
     9061 tatattgttt ctgcccccag tggtgcgggt aaatccagcc tgattcaggc tttattaaaa
     9121 acccaaccgt tgtatgacac ccaggtttct gtttcacaca ccacacgcca accgcgtcct
     9181 ggtgaagtcc acggtgaaca ttatttcttt gttaatcatg atgaatttaa agaaatgatt
     9241 agcagagatg cgttcctcga acacgcagaa gtttttggta attactatgg cacttcgcgt
     9301 gaggccattg agcaagtact ggcgaccggt gtcgatgttt ttctcgatat cgactggcag
     9361 ggcgcgcagc aaattcgcca gaagatgccg cacgcgcgga gtatctttat tttaccgccg
     9421 tccaaaattg aactggaccg ccgtctacgc ggtcgcggtc aggacagcga agaggtcatt
     9481 gcaaagcgta tggcgcaagc tgttgcagaa atgagccatt acgccgaata tgattatctg
     9541 attgtgaatg atgacttcga taccgcgttg accgatttga agaccattat tcgcgccgaa
     9601 cgtctgcgca tgagccgcca aaagcagcgt catgacgctt taatcagcaa attgttggca
     9661 gactgaacct gatttcagta tcatgcccag tcatttcttc acctgtggag ctttttaagt
     9721 atggcacgcg taactgttca ggacgctgta gagaaaattg gtaaccgttt tgacctggta
     9781 ctggtcgccg cgcgtcgcgc tcgtcagatg caggtaggcg gaaaggatcc gctggtaccg
     9841 gaagaaaacg ataaaaccac tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac
     9901 aaccagatcc tcgacgttcg cgaacgccag gaacagcaag agcaggaagc cgctgaatta
     9961 caagccgtta ccgctattgc tgaaggtcgt cgttaatcac aaagcgggtc gcccttgtat
    10021 ctgtttgaaa gcctgaatca actgattcaa acctacctgc cggaagacca aatcaagcgt
    10081 ctgcggcagg cgtatctcgt tgcacgtgat gctcacgagg ggcaaacacg ttcaagcggt
    10141 gaaccctaca tcacgcaccc ggtagcggtt gcctgcattc tggccgagat gaaactcgac
    10201 tatgaaacgc tgatggcggc gctgctgcat gacgtgattg aagatacacc cgccacctac
    10261 caggatatgg aacagctttt tggtaaaagc gtcgccgagc tggtagaggg ggtatcgaaa
    10321 cttgataaac tcaagttccg cgataagaaa gaggcgcagg ccgaaaactt tcgcaagatg
    10381 attatggcga tggtgcagga tatccgcgtc atcctcatca aacttgccga ccgtacccac
    10441 aacatgcgca cgctgggctc acttcgcccg gacaaacgtc gccgcatcgc ccgtgaaact
    10501 ctcgaaattt acagcccgct ggcgcaccgt ttaggtatcc accacattaa aaccgaactc
    10561 gaagagctgg gttttgaggc gctgtatccc aaccgttatc gcgtaatcaa agaagtggtg
    10621 aaagccgcgc gcggcaaccg taaagagatg atccagaaga ttctttctga aatcgaaggg
    10681 cgtttgcagg aagcgggaat accgtgccgc gtcagtggtc gcgaaaagca tctttattcg
    10741 atttactgca aaatggtgct caaagagcag cgttttcact caatcatgga catctacgct
    10801 ttccgcgtga tcgtcaatga ttccgacacc tgttatcgcg tgctcgggca aatgcatagc
    10861 ctgtacaagc cgcgtccggg tcgcgtgaaa gactacatcg ccattccaaa agcgaacggc
    10921 tatcagtctt tgcacacctc gatgatcggc ccgcacggtg tgccggttga ggtccagatc
    10981 cgtactgaag atatggatca gatggcggag atgggtgttg ccgcgcactg ggcttataaa
    11041 gagcacggcg aaaccagcac caccgcgcaa atccgcgccc agcgctggat gcaaagcctg
    11101 ctggagctgc aacagagcgc cggtagttcg tttgaattta tcgagagcgt taaatccgat
    11161 ctcttcccgg atgagattta cgttttcaca ccggaagggc gaattgtcga gctgcctgcc
    11221 ggtgcaacgc ccgtcgactt cgcttatgca gtgcataccg atatcggtca tgcctgcgtg
    11281 ggcgcacgcg ttgaccgcca gccttacccg ctgtcgcagc cgcttaccag cggtcaaacc
    11341 gttgaaatca ttaccgcacc gggcgctcgc ccgaatgccg cgtggctgaa ctttgtcgtt
    11401 agctcaaaag cgcgcgccaa aattcgtcag ttgctgaaaa acctcaagcg tgatgattct
    11461 gtaagcctgg gccgtcgtct gctcaaccat gctttgggtg gtagccgtaa gctgaatgaa
    11521 atcccgcagg aaaatattca gcgcgagctg gatcgcatga agctggcaac gcttgacgat
    11581 ctgctggcag aaatcggact tggtaacgca atgagcgtgg tggtcgcgaa aaatctgcaa
    11641 catggggacg cctccattcc accggcaacc caaagccacg gacatctgcc cattaaaggt
    11701 gccgatggcg tgctgatcac ctttgcgaaa tgctgccgcc ctattcctgg cgacccgatt
    11761 atcgcccacg tcagccccgg taaaggtctg gtgatccacc atgaatcctg ccgtaatatc
    11821 cgtggctacc agaaagagcc agagaagttt atggctgtgg aatgggataa agagacggcg
    11881 caggagttca tcaccgaaat caaggtggag atgttcaatc atcagggcgc gctggcaaac
    11941 ctgacggcgg caattaacac cacgacttcg aatattcaaa gtttgaatac ggaagagaaa
    12001 gatggtcgcg tctacagcgc ctttattcgt ctgaccgccc gtgaccgtgt gcatctggcg
    12061 aatatcatgc gcaaaatccg cgtgatgcca gacgtgatta aagtcacccg aaaccgaaat
    12121 taatgtttta tgaacccaac acgttatgca cgcatctgcg aaatgctcgc caggcggcag
    12181 cctgatctga ccgtctgcat ggagcaggtc cacaaacctc ataacgtttc tgcgattatt
    12241 cgtaccgcag atgccgttgg cgtacatgaa gttcacgccg tctggcctgg tagccgcatg
    12301 cgcaccatgg cttcggcagc ggcgggtagt aacagctggg tacaggtgaa aacacaccgc
    12361 accattggcg atgccgtcgc tcatctgaaa ggccagggca tgcagattct ggcaacccat
    12421 ctttctgata acgctgtcga tttccgcgaa attgattaca ctcgcccgac ctgcattttg
    12481 atgggacagg agaaaacggg catcacgcag gaagcattgg ccctggcgga tcaggacatc
    12541 atcattccga tgatcggcat ggtgcagtcg ctgaatgttt ccgttgcctc agccctcatt
    12601 ctttacgaag cccagcgtca gcggcaaaat gcaggcatgt acctgcgtga aaacagcatg
    12661 ttgccggaag cagagcaaca acgcctgttg tttgaaggcg gctatccggt gctggcgaaa
    12721 gtcgcaaaac gcaaaggcct gccttatccc cacgtcaatc agcaaggcga gatcgaagct
    12781 gatgccgact ggtgggctac tatgcaggct gcagggtaag tgccatgaaa ggtcgcctgt
    12841 tagatgctgt cccactcagt tccctaacgg gcgttggcgc agcacttagt aacaaactgg
    12901 cgaaaatcaa cctgcatacc gtgcaggatc tactcttaca ccttcccctg cgctacgaag
    12961 atcgcaccca tctctacccc atcggagaac tactgccggg cgtttatgcc acggtggaag
    13021 gcgaagtgct gaactgcaat atctccttcg gcggtcggcg gatgatgacc tgccagatca
    13081 gcgacggttc cggcatcctc accatgcgct ttttcaattt cagcgcggca atgaaaaata
    13141 gcctggcgac gggccgccgt gtactggctt atggcgaagc aaagcgcggt aaatatggtg
    13201 cggagatgat ccacccggaa taccgcgtgc agggcgatct cagcacgcca gaattacagg
    13261 aaacgctcac gccggtttat ccaacaacgg aaggcgtaaa gcaggccacg ctgcgtaaat
    13321 taaccgacca ggcgctggat ctgctcgaca cctgcgccat tgaagaactc ctgccgccgg
    13381 aactgtcaca aggaatgatg acgctaccgg aagcgttgcg cactttgcac cgcccgccac
    13441 cgacgctaca gcttagcgat ctggaaaccg ggcagcatcc ggcgcaacgt cgtctgattc
    13501 tggaagaact gctggcgcac aacctcagca tgttagcctt acgtgccgga gcacagcgtt
    13561 ttcatgccca gccgctgagc gccaatgatg cgctgaaaaa taaactcctc gcagccttac
    13621 cgttcaagcc aacgggcgca caggcacgcg tagtggcgga gatcgagcgc gatatggcgc
    13681 tggatgtgcc gatgatgcgc ctggtacagg gcgatgtagg ttccggtaaa acgctggtcg
    13741 ccgccctcgc cgcgctgcgt gcgattgccc acggcaaaca ggtggcattg atggcaccaa
    13801 ccgaattact cgccgaacag cacgccaata acttccgcaa ctggtttgaa ccgctcggta
    13861 tcgaagtggg ctggctcgcc ggtaagcaga aaggcaaagc gcgactgtct cagcaggaag
    13921 ccatcgccag cggtcaggtg cagatgattg ttggtacgca cgccatcttc caggaacagg
    13981 tgcagtttaa cggtctggcg ctggtgatta tcgacgaaca acatcgcttt ggcgtgcatc
    14041 agcgtctggc gttatgggag aaaggccagc agcaaggctt ccatccgcat cagttgatca
    14101 tgaccgccac gccgatcccc cgcacgctgg caatgacggc gtatgccgat ctcgatacct
    14161 cggtgataga tgaactgccg ccaggccgca cgccagtgac tacggtcgct attcctgata
    14221 ctcgccgtac cgacatcatt gaccgcgtgc gccacgcctg catgactgaa ggtcgtcagg
    14281 cgtactgggt ttgtacgttg attgaagagt cagagttact ggaagcgcag gcggcggaag
    14341 ctacctggga agagttgaaa ctggcgctac cagaattgaa tgttggcctg gtacacgggc
    14401 ggatgaaacc agccgagaaa caggcggtga tggcgtcgtt taaacaaggt gagctacatc
    14461 tgctggttgc cactaccgtt attgaagtcg gtgttgatgt gcctaacgcc agcctgatga
    14521 ttatcgaaaa ccctgagcgt ctgggtctgg cgcagttaca ccagctgcgc gggcgcgtag
    14581 gtcgtggcgc ggtggcttct cactgcgtgc tactctacaa aacgccgctt tctaaaacgg
    14641 cgcaaattcg cctgcaagtg ctgcgcgaca gtaacgacgg ttttgtgatt gcgcaaaaag
    14701 atctggagat tcgcggccct ggcgaattgt taggcacgcg tcagacgggt aatgctgaat
    14761 ttaaagtggc ggatttactg cgcgatcagg cgatgatccc ggaagttcag cgcctggcac
    14821 gccatattca cgaacgttac ccacaacagg caaaagccct gatagaacgc tggatgccgg
    14881 agacggaacg ttactcgaat gcgtaaaagg cggcagtgag aagaccgcca tttcaggtta
    14941 ccctaccttc ctgccggatg cgattcatca ccctacaaat tcaataaatt atgaatcaat
    15001 acgcaggctt gataagcgaa gcgtatcagg catttgtctt tgttgcggcg gcacttctac
    15061 cgccgcttca tcggttaacc ggcaaaaatc ggcaacatca aatacaactt aattaccagc
    15121 gcattgacga tatcgataaa gaacgcaccg accatcggca ccaccaaaaa cgccatgtgc
    15181 gacgggccaa agcgttcagt aatcgcctgc atgttggcga ttgccgttgg cgttgcaccg
    15241 aggccaaaac cacagtgacc tgcagccagc actgccgcat cgtagttttt gcccatcatg
    15301 cgccaggtaa cgaagatggc atacaacgcc atgaagatgg tctgtaccac cagaatcgcc
    15361 agcatcggca gcgccagcga agccagctcc cacagtttca gccccatcaa cgccatcgcc
    15421 aggaacaacg acaagcttac gttacccagc acggataccg cacgctcaaa gacgcggtaa
    15481 aagcccatta ttgacagacc gttgctcaga atcacgccaa caaacagtac acagacgaag
    15541 gtcggcagtt caaaagcagt gccagccaaa agttgcgcaa caattttccc caccgtcagg
    15601 cagatagcaa tcagcgcgat agtttcaatc agcaccaacg aggtgatcat gcgtcccaca
    15661 tccggctttt caaacgccgt cgggacttcc tggtcatccg gaataccgtt cggcgtggtg
    15721 gagtgtttca ccagatagcg cgccaccgga ccgccaatca agccgcccag caccagaccg
    15781 aacgttgcac aggccatcgc cacttccgtc gcattggtga agccataacg ttcaatgaac
    15841 aatttactcc acgcagcgcc cgtaccgtga ccgccggaaa gcgtaataga accggccaac
    15901 agccccatca gcggatcaag ccctaacagg ctagccatac caatgccaat ggcattttgc
    15961 atcaccaaca gaccaacaac cacaatcaag aagatgccaa ccacacgccc accggcacgc
    16021 aaactggcaa tgttggcgtt caggccaatg gtggcgaaga aagccagcat taacggatcg
    16081 cgcagggaca tatcaaagtt gacttcccag cccatgcttt ttttcagtac tagtagcgcc
    16141 agcgccacca acaaaccacc cgcaacaggt tccggtatgg tgtatttctt caaaaaggag
    16201 acggaatgga ccaacttacg cccgagcagc aacgtcagcg ttgcggcaac aagcgttgct
    16261 aaagtatcga gatgaaacat agttactcct ttgtatccgc atcactcttt catactcgtc
    16321 atacttcatc gcgcagtggc aacttcatga actatgacgg gtatcgagtt atgctttcct
    16381 gggtcaaatt ctcagctgtt aatgaacaaa tggcatggat tttaagcaga aaaataccaa
    16441 aagttatata aaaaagtgca tttatctcat ttaaatattt agatgaggat gtggcaaacg
    16501 tttgcttttc tctttcggtc agctaaaatg cccgctttgc taccacggga ttgttttcga
    16561 tgtctgtttc caccctcgag tcagaaaatg cgcaaccggt tgcgcagact caaaacagcg
    16621 aactgattta ccgtcttgaa gatcgtccgc cgcttcctca aaccctgttt gccgcctgtc
    16681 agcatctgct ggcgatgttc gttgcggtga tcacgccagc gctattaatc tgccaggcgc
    16741 tgggtttacc ggcacaagac acgcaacaca ttattagtat gtcgctgttt gcctccggtg
    16801 tggcatcgat tattcaaatt aaggcctggg gtccggttgg ctccgggctg ttgtctattc
    16861 agggcaccag cttcaacttt gttgccccgc tgattatggg cggtaccgcg ctgaaaaccg
    16921 gtggtgctga tgttcctacc atgatggcgg ctttgttcgg cacgttgatg ctggcaagtt
    16981 gcaccgagat ggtgatctcc cgcgttctgc atctggcgcg ccgcattatt acgccgctgg
    17041 tttctggcgt tgtggtgatg attatcggcc tgtcgctaat tcaggtgggg ctgacctcca
    17101 ttggcggcgg ttacgcagcc atgagcgata acaccttcgg cgcaccgaaa aatctgctgc
    17161 tggcaggcgt ggttttagcc ttaattatcc tgcttaaccg tcaacgtaac ccttacttac
    17221 gcgtggcctc actggtaatt gcgatggcgg ccggatatgc gctggcgtgg tttatgggca
    17281 tgttgccaga aagcaacgaa ccgatgacgc aagaactgat tatggtgcca acgccgctct
    17341 attacggtct tggcattgaa tggagtctgc tgctgccgct gatgctggtc tttatgatca
    17401 cttcgctgga aaccattggc gatatcacgg cgacctctga cgtttccgaa cagccagtgt
    17461 ccggtccgct gtacatgaaa cgcctgaaag gcggcgtgct ggcaaacggc ctgaactcgt
    17521 ttgtttcggc ggtgtttaac accttcccga actcctgctt cgggcaaaac aacggagtga
    17581 tccagttgac cggtgttgcc agccgctatg tcggttttgt cgtcgcgctg atgttgatcg
    17641 tgctgggtct gttcccggca gtgagcggtt ttgtacaaca cattccagaa ccggttctgg
    17701 gcggcgcaac gcttgtaatg tttggcacca tcgccgcctc cggtgtgcgt atcgtttctc
    17761 gtgagccgct gaaccgtcgg gcgattctga ttatcgcgct gtcgctggcg gttggtctgg
    17821 gcgtgtctca gcagccgctg attttgcagt ttgcccctga atggctgaaa aacctgctct
    17881 cctccgggat cgccgcgggc ggtattactg ccatcgtgct gaatctgatt ttcccaccag
    17941 aaaaacagta attcactggc ggcggtaaga taattaccgc cgcagtctca cctctttaac
    18001 cattcccgcc ttgaggattg cgcgtaaatc gtgcataact cccttatgtg catttcacgg
    18061 gatggaagac catgaaattt attgggaagc tgcttctcta cattctcatc gctctgttag
    18121 tggcgatcgc tggcctctat tttcttctgc aaacccgctg gggagcagaa catatcagcg
    18181 catgggtttc cgagaatagc gactatcatc tggccttcgg ggcgatggtt caccgttttt
    18241 ccgcgccatc tcatatcgtg ctggagaatg tcacgtttgg tcgtgatggc cagcccgcga
    18301 ccctggtggc caaaagtgtc gacattgcgc taagtagtcg gcaactgacc gaaccacgcc
    18361 atgtcgatac catcctgctg gaaaacggga cgctgaatct caccgaccag accgcgccgc
    18421 taccgttcaa agccgatcgt ctgcaactgc gtgatatggc gtttaatagc ccgaatagcg
    18481 aatggaaact gagcgcgcag cgggtaaatg gcggcgtggt tccgtggtca ccagaagccg
    18541 gtaaagtgct gggtacgaag gcgcagattc agtttagtgc cggatcgctt tcgctcaatg
    18601 atgttcctgc caccaatgta ctgattgaag gcagtattga taacgatcgc gttacgctga
    18661 ctaacctggg tgccgacatc gcgcgcggga cattaaccgg aaacgcgcag cgtaacgccg
    18721 acggcagctg gcaagtggaa aacctgcgca tggcggatat ccgtctacaa agcgaaaaat
    18781 cgctaaccga cttctttgcg ccattacgct ctgtcccgtc gttgcagatt ggtcgcctgg
    18841 aagtgatcga tgctcgtttg caaggtccgg actgggcggt gaccgacctc gatctcagct
    18901 tgcgcaacat gaccttcagt aaagatgact ggcagacaca agaaggcaaa ctgtcgatga
    18961 acgctagcga gttcatttat ggttcgctgc atttatttga cccgattata aacgcggaat
    19021 tttctccgca gggcgtagcg ctgcgccagt tcaccagccg ctgggaaggg ggtatggtca
    19081 gaacgtcagg gaactggctg cgtgacggga aaacgttgat ccttgatgat gcggcaattg
    19141 ccggactgga atataccttg ccgaaaaact ggcaacagtt gtggatggaa acgacacccg
    19201 gttggttaaa cagcctgcaa ctgaagagat ttagcgccag ccgcaatctg atcattgata
    19261 tcgaccctga cttcccgtgg cagctcaccg cgctcgatgg ttacggtgcc aacctgacgc
    19321 tggttaccga tcataaatgg ggcgtctgga gtggctcggc gaatctgaat gccgccgccg
    19381 cgacgtttaa tcgtgttgat gtccgtcgtc cgtcgctggc gctaaccgcc aacagcagca
    19441 cggtgaatat cagcgaactg agtgcattta ctgaaaaagg cattctggaa gccaccgcca
    19501 gtgtttcaca aacgccacaa cgtcagacac atatcagcct gaatggacgc ggtgtgccgg
    19561 tgaatatttt gcaacagtgg ggatggcctg aattaccgtt gactggcgac ggcaatattc
    19621 agcttaccgc cagtggcgat attcaggcca atgtcccgtt gaaacctacg gttagcgggc
    19681 aattgcatgc cgtgaacgcc gcaaagcacc aagtgactca aaccatgaat gcgggcgtcg
    19741 tttccagcgg tgaagttaca tcggcagagc tggtgcagta aacatcaatc tcgccgggta
    19801 cgactcagac gtacccggca ttcttcaata gattacaacg taattgtcag cgcattccct
    19861 tgaggcgtaa ccaccagccc ctgctcactt tcagcctgcg aaccgccttg cagaccattt
    19921 actttcacaa tattgcgcaa gcacagtgtc cagttcctcg cctcgcccgc accagtcaca
    19981 gtaatcgtgt taccagtacg tgttgctttt agagtaaaga tcaccgaacc gtcagcagcg
    20041 ggcacttcac atacagcttc atgcccgtct tgcagattaa agagatggaa tgccgtgcct
    20101 tcgtgccacg cgtaatcggg acgttgatcg ttgttgccca gcgccaatag ggtgttatca
    20161 cgcacataaa cgggcagact caggaagccg tgctgctgtt tatgccagcg actaccgtcg
    20221 agctcattgt tgtgccacag gtgtgtccag cgaccttccg gcaagtagaa ctgcacctcg
    20281 cccgcctcag tgaacaccgg agcaaccatc acgttgtcgc ctagcatgta ttgacggtca
    20341 aggtaatcac aagccggatc gtccgggaac tccatcatca tggcccgcat catcggcgta
    20401 ccccgcgctt tcgcacgcgc agcttcacga tacagatacg gcatcatgcg gcatttcagt
    20461 tgcgtgaaga agcgcaccac atcacaggac tcatcatcgt aggcccacgg cacacgataa
    20521 gatttgctgc catgtaaacg actatggctg gagagcaaac caaacgcgca ccagcgtttg
    20581 taaacgtgtg ccggcgcggt attttcaaag ccgccgatat cgtggctcca gaagccaaaa
    20641 cctgaaaggc caatagacaa accaccgcgc aggctttccg ccattgattc gtagttagcg
    20701 taacaatcgc caccccagtg taccgggaat ttctgcgcac cgacggaggc cgagcgggca
    20761 aacaagacag cttcttcctc accaacggtg tccttgagca cgttccacac cagttcgttg
    20821 tagatgtacg cataatggtt atgcattttc tgcggatcgg aaccgtcaaa ccactgaaca
    20881 tcagttggga tacgttcgcc aaagtcggtc ttaaagcaat caacgcccat cgcgaccaga
    20941 cctttcagtt tgtcggcgta ccatttgcag gcatccggat tggtaaagtc ataaatcgcc
    21001 aga
//
LOCUS       DAZPEP010000060        20867 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZPEP010000060 DAZPEP010000000
VERSION     DAZPEP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20867
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.060"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHUT9_002216"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="ACHUT9_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFR1348685.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="ACHUT9_002217"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="ACHUT9_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHUT9_002218"
     CDS             complement(2290..4248)
                     /gene="acs"
                     /locus_tag="ACHUT9_002218"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFR1348686.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLTCGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHUT9_002219"
     CDS             4642..6078
                     /gene="nrfA"
                     /locus_tag="ACHUT9_002219"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFR1348687.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHUT9_002220"
     CDS             6123..6689
                     /gene="nrfB"
                     /locus_tag="ACHUT9_002220"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFR1348688.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHUT9_002221"
     CDS             6686..7357
                     /gene="nrfC"
                     /locus_tag="ACHUT9_002221"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFR1348689.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHUT9_002222"
     CDS             7354..8310
                     /gene="nrfD"
                     /locus_tag="ACHUT9_002222"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFR1348690.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8330..10048
                     /locus_tag="ACHUT9_002223"
     CDS             8330..10048
                     /locus_tag="ACHUT9_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFR1348691.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHUT9_002224"
     CDS             10041..10424
                     /gene="nrfF"
                     /locus_tag="ACHUT9_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFR1348692.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHUT9_002225"
     CDS             10421..11017
                     /gene="nrfG"
                     /locus_tag="ACHUT9_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFR1348693.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11359..12672
                     /gene="gltP"
                     /locus_tag="ACHUT9_002226"
     CDS             11359..12672
                     /gene="gltP"
                     /locus_tag="ACHUT9_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFR1348694.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHRELLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHUT9_002227"
     CDS             complement(12751..13440)
                     /gene="yjcO"
                     /locus_tag="ACHUT9_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFR1348695.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHUT9_002228"
     CDS             complement(13534..15681)
                     /gene="fdhF"
                     /locus_tag="ACHUT9_002228"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15262..15264),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFR1348696.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMDHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHUT9_002229"
                     /pseudo
     CDS             complement(15879..17345)
                     /gene="mdtP"
                     /locus_tag="ACHUT9_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
     gene            complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHUT9_002230"
     CDS             complement(17342..19393)
                     /gene="mdtO"
                     /locus_tag="ACHUT9_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFR1348697.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHUT9_002231"
     CDS             complement(19393..20424)
                     /gene="mdtN"
                     /locus_tag="ACHUT9_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFR1348698.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20443..20718)
                     /locus_tag="ACHUT9_002232"
     CDS             complement(20443..20718)
                     /locus_tag="ACHUT9_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFR1348699.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4703 a         5524 c         5702 g         4938 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaatcaatg
     2221 cctgatgcga cgctgtcgcg tcttatcagg cctacaaacc gttaccgact cgcatccggc
     2281 aattgtgggt tacgatggca tcgcgatagc ctgcttctct tcaagcagct tctcgactac
     2341 gccaggatcg gcaagcgtcg aggtatcgcc caggttgctg gtatcgcccg ccgcaatttt
     2401 gcgcagaata cggcgcataa ttttgccgga gcgggtttta ggcagggagt cggtccagtg
     2461 cagcacgtct ggcgtcgcca gcgggccaat ctctttacgc acccagttgc ggacttctgc
     2521 gtacagttct ggtgacggtt cctccccgtg attaagcgtg acgtaggcgt agatcgcctg
     2581 acctttaata ttgtgcggaa tacctactac ggcggcttcg gcaatcttcg gatgcgccac
     2641 cagcgccgac tcaatctctg ccgtccccag acggtgaccg gagacgttca gcacgtcgtc
     2701 cacacgcccg gtgatccagt aatagccatc ttcatcgcga cgcgcgccgt cgccgctgaa
     2761 atacatattt ttgaaggtgg agaagtaggt ctgttcaaaa cgttcgtgat cgccaaacag
     2821 cgtacgcgcc tgacccggcc aggagtcggt gatcaccagg ctgccttcgg tagcgccttc
     2881 cagcgggtta ccttcgttat cgaccagcgc cggttgcacg ccgaagaacg gacgtgttgc
     2941 cgaaccggct ttcagctcgg tagcgccagg cagcggcgtg atcatgaaac cgccagtttc
     3001 ggtctgccac caggtatcga ccaccggaca tttctcgttg ccgatttttt tccagtacca
     3061 ctcccacgct tccgggttaa ttggctcgcc cacggaaccg agaatgcgca gcgacgaacg
     3121 gtcggtaccc tcgatcgctt tatcgccttc cgccatcagc gcgcggatcg ccgtgggtgc
     3181 ggtatagaga atattgacct gatgcttgtc caccacctgc gccatacggg caggcgtcgg
     3241 ccagttgggt acgccttcaa acatcagcgt ggtcgcaccg caggtcagcg ggccgtacag
     3301 caagtaactg tgtccggtca cccagcccac atcggcggtg caccagtaga tatcacccgg
     3361 atgataatca aagacatatt taaaggtcag cgccgcgtac accagataac cgccggtagt
     3421 atgcagcaca ccttttggct taccggtaga accggaggtg tagagaataa acagcggatc
     3481 ttcggcgttc atcttttccg cctggtgctg atcgctcgct tgctcaacca ggtcgtgcca
     3541 ccacaggtcg cgcccttcct gccagtcaat tttcccgcca gtacgcttca gtaccaccac
     3601 atgctctacg ctggtgacgt tcgggttttt cagcgcgtca tcaacgtttt tcttcagcgg
     3661 aatactgcgc ccggcacgca caccttcgtc ggaagtgatc accagtcgtg agttggaatc
     3721 aataatgcgc ccggcaacgg cttccggcga gaagccgccg aaaatcaccg aatgcaccgc
     3781 gccaatgcgg gcgcaggcca gcatcgcaac cgcggcttcc ggcaccatcg gcatataaat
     3841 cgccaccaca tcaccttttt taatgcccag ctcgagcagg gtattggcga agcggcagac
     3901 gtcgcggtgc agctctttat agctgatatg tttgctctgg ctggcgtcgt cgccttccca
     3961 gatgatggcg gtacgatcgc cgttttcttg cagatggcgg tcaaggcagt ttgccgccag
     4021 attcagcgtg ccgtcctcgt accatttaat ggacacatta ccgggggcaa aggaggtgtt
     4081 tttcaccttc tggtaaggtt tgatccagtc aagaattttt ccctgttcgc cccagaaggt
     4141 atcaggtacg ttaatagatt gttgatacat cgcctcgtac tgctgagggt ttatcaggca
     4201 acggtctgcg atgttggcag gaatggtgtg tttgtgaatt tggctcatgc ttttgttctc
     4261 cttgtagggt gttaataata tgtggcataa gcgttaaatg taggggtatt ggcagttttg
     4321 tttagtattt gggcgacaga tcacgcaaaa gtagaattgt gcaaataaac ggcagggtaa
     4381 tttttgaagg tcagcaacaa aagttgatta attctttgag gaacatgcag ttatgcattg
     4441 ctgttgaaaa agaggaagat actaactaac tctaaagtgg tattttacat gcacttacaa
     4501 ttgattaaag acaacattca cagtgtggtt atttgttaca cataggggcg agcaatgtca
     4561 tgacagtgta ggtgcggtta ctgtcgtgaa aaacaataaa aaccgccatt gtaacaatgg
     4621 cgcaattcgg atgaagcccc tatgacaagg ataaaaataa acgcacgccg tatcttcagc
     4681 ttattgattc cttttttctt tttcacttct gttcacgctg aacaaacggc agctcccgca
     4741 aaacctgtaa ctgtggaagc gaagaatgaa acctttgccc cgcagcatcc cgatcaatat
     4801 ctctcctgga aagccacctc ggaacagtca gagcgtgttg acgccctggc ggaagatcca
     4861 cggctggtga tcctgtgggc ggggtatccc ttctcgcgcg attacaacaa gccgcgtgga
     4921 catgcctttg ctgtgaccga tgtgcgtgaa accctgcgta ccggtgcgcc gaaaaacgct
     4981 gaagatggtc cgctaccgat ggcgtgctgg agttgtaaaa gcccggatgt ggcgcgtctg
     5041 atccagaaag acggcgaaga tggctacttc cacggtaagt gggcgcgcgg cggcccggaa
     5101 atcgtcaaca acttaggttg tgccgactgc cataacaccg cctcaccaga gttcgccaaa
     5161 ggcaaaccgg agttaaccct ttcccgtccg tatgcggctc gcgcgatgga agccattggt
     5221 aaaccttttg agaaagccgg acgtttcgac cagcaatcga tggtttgcgg tcagtgccat
     5281 gtggagtatt acttcgacgg caaaaacaaa gcggttaaat tcccgtggga tgacggcatg
     5341 aaagtcgaaa atatggagca gtattacgac aaaattgcct tctctgactg gactaactcc
     5401 ctgtcgaaaa cgccaatgct gaaagcgcag cacccggaat atgaaacctg gacagcgggc
     5461 attcacggta aaaacaacgt gacctgtatc gactgccata tgccaaaagt gcagaacgcc
     5521 gaaggcaaac tctataccga ccataaaatt ggtaatccgt ttgataactt cgcccagact
     5581 tgtgcgaact gccataccca ggacaaagct gccttgcaaa aagtggtcgc ggaacgtaag
     5641 cagtcgatta acgacctgaa aatcaaggtt gaagatcaac tggttcacgc tcacttcgaa
     5701 gcgaaagcag cgctggatgc aggcgcgacg gaagctgaaa tgaagccaat tcaggacgat
     5761 atccgtcatg cccagtggcg ctgggatctg gcgatcgctt cccacggcat tcatatgcac
     5821 gcaccggaag aaggtttacg gatgctcggt acggcgatgg ataaagcggc ggatgcacgc
     5881 accaaactgg cgcgcctgct ggcgaccaaa ggcatcaccc atgaaatcca gatcccggat
     5941 atctcaacca aagagaaagc ccagcaggcc attggcctga acatggaaca aatcaaggcc
     6001 gagaagcagg acttcatcaa aacggtgatc ccgcagtggg aagaacaggc acgtaaaaac
     6061 ggtctgttaa gccaataacc ccgttccgcc tcgcaagggg cggaaaacac aatggagtga
     6121 atatgagcgt attacgttcg ttattaactg ccggggtgct ggcgtcgggc ctgttgtgga
     6181 gcctgaacgg aattaccgct acccctgcgg cgcaggcatc tgatgatcgc tacgaagtta
     6241 cccagcagcg taacccggat gccgcctgtc tggactgtca taaaccagat accgaaggta
     6301 tgcatggcaa acatgcttcc gttatcaacc cgaataacaa actgccggtc acctgcacca
     6361 actgtcacgg ccagccatca ccgcaacacc gcgaaggggt gaaagatgtg atgcgcttta
     6421 acgagccgat gtacaaggtt ggagagcaga acagcgtctg tatgtcctgt catctgccgg
     6481 aacagttgca aaaagcgttc tggccgcacg atgtccacgt aaccaaagtg gcgtgcgcca
     6541 gctgccattc cctgcatcca cagcaagata cgatgcagac gttaagtgac aaaggacgga
     6601 ttaagatttg cgtcgattgc cacagcgatc agcgcaccaa tccgaacttt aacccggcgt
     6661 ctgttccgtt gcttaaggag cagccatgac ctggtctcgt cgccagtttc tcaccggcgt
     6721 cggcgtgctg gcagccgtca gcggcaccgc agggcgcgtg gtggcgaaaa cgttgaatat
     6781 caatggggtg cgttacggca tggtgcatga cgagtcgtta tgcatcggct gtaccgcctg
     6841 tatggatgct tgtcgggaag tgaacaaggt gccggaaggc gtctcgcgcc tgacgattat
     6901 tcgtagcgag ccgcagggcg aatttcctga tgtgaaatat cgcttcttcc gtaagtcttg
     6961 ccagcactgc gatcatgcgc cgtgcgttga cgtctgcccg accggtgcgt cttttcgtga
     7021 tgccgctagc gggattgtcg atgttaatcc tgatctctgc gtcggttgtc agtactgcat
     7081 cgccgcctgt ccgtaccgcg tgcgctttat ccatccggtc acgaagacgg cggacaaatg
     7141 cgatttctgc cgtaaaacca atttgcaggc cggtaagctg cccgcgtgtg ttgaggcttg
     7201 cccgacgaag gcgctgacgt ttggcaatct ggacgatcct aacagtgaga tttcgcaact
     7261 gctgcgccag aagcctactt accgctacaa gctggcgctg ggaaccaaac cgaagctgta
     7321 ccgcgtaccg tttaaatacg gggaggtgag ccaatgacgc agacttccgc atttcatttt
     7381 gaatcgctgg tgtgggactg gccgattgcc atctacctgt ttttgattgg tatttctgcc
     7441 ggtctggtga cgctggccgt gctgttacgt cgcttctacc cgcaggcggg cggtgcagac
     7501 agtacgttgc tgcgcaccac gctgattgtc gggccgggcg cggtgatcct cggtctgttg
     7561 atcctcgtct tccacctgac aagaccgtgg accttctgga agctgatgtt ccactacagt
     7621 tttacctcgg tgatgtcgat gggggtgatg ctgtttcagc tctacatggt ggtgctggtg
     7681 ctgtggctgg cgaaaatctt tgaacatgat ttgcttgccc tgcaacaacg ctggttgccg
     7741 aagctgggga tcgtgcaaaa ggttctgagc ctgctgacgc ccgttcatcg cggactggaa
     7801 acgctgatgc tggtgttggc ggtgctgctg ggggcttata ccggctttct gctgtcggcg
     7861 ctgaaatcgt atccgttcct caataacccg atcctgccgg tgctgttcct cttctccggc
     7921 atctcgtccg gtgcggcggt ggcgctgatc gccatggcga tacgccaacg cagtaacccg
     7981 cattccacgg aagcgcactt tgttcaccgg atggaaatcc ccgtggtatg gggtgaaatc
     8041 ttcctgctgg tggcgttttt tgtcggtctg gcgctgggcg atgacggtaa agttcgtgcg
     8101 ctggtggcgg cattaggtgg cggtttctgg acgtggtggt tctggcttgg tgtcgccggg
     8161 ctggggctga ttgtgccaat gttgctcaaa ccgtgggtca atcgcagttc cggcattcct
     8221 gccgtgctgg cggcgtgtgg ggccagtctg gtcggcgtgt tgatgctgcg ctttttcatt
     8281 ctctacgccg gacagttgac ggtggcgtaa gccagaaaag aggtggtttc tggacgtatt
     8341 ccttcctgaa gtcggttttc tggcgttgtt gttaagtctc ggggtcaacg tgttgacccc
     8401 gttgacggcc tttgcgggag tgcggttgcg ctggcctgcc atgatgcgac tcacttgcat
     8461 cggcattctg gcgcagttcg cgctcctgct gctcgccttt ggcgtactga cgtattgttt
     8521 tctcatcagc gatttctcgg tcatttatgt cgcgcaacat agctatagcc tgctgtcgtg
     8581 ggaattcaag ctggcagcgg tgtggggcgg ccatgaaggt tcgctgctgc tttgggtgct
     8641 gctgctttcc gcctggagcg cgctgtttgc ctggcattat cggcagcaaa ccgatccgct
     8701 atttccgctg acgttagccg ttttatctct catgctcgcc gcactgctac tgtttgtggt
     8761 gctgtggtcc gatcccttcg tgcggatatt tccaccagca atcgaaggcc gcgatctcaa
     8821 tccgatgctg caacatcccg gtcttatctt tcatccaccg ctgctttacc ttggctatgg
     8881 cggtttgatg gtagcggcga gcgtggcgct ggcgagtcta ctgcgcggcg agtttgatgc
     8941 ggccagcgcc cgaatttgct ggcgctgggc gttacctggc tggagtgcat taacggcggg
     9001 gatcatcctc ggttcctggt gggcctactg cgaactcggc tggggcggct ggtggttctg
     9061 ggatccggtg gaaaatgcct ctttattacc ctggctttct gccactgcgc tgctgcacag
     9121 tttatccctg acacgccagc gggggatttt ttgccactgg tcgctgttgc tggcgatagt
     9181 tactctgatg ctgtcgctgc tgggcacctt aattgtccgt tctggcattc tggtttcggt
     9241 tcatgcgttc gcactggata acgtccgcgc cgtgccgttg ttcagcctgt ttgcactgat
     9301 tagccttgcg tctttggctc tgtatggctg gcgagcgcgg gacggtggcc cggcggtgcg
     9361 tttttcgggg ttatcgcggg aaatgttaat cctcgctacg ctgttgctgt tttgcgcagt
     9421 gctactgatc gtgctggtgg gaacgcttta tccgatgatt tacggtctgc tgggctgggg
     9481 acgcctctcc gttggcgcgc cgtattttaa ccgcgcgacg ttaccgtttg gtctgttgat
     9541 gctggtggtg attgtgctgg cgacgtttgt ctctggcaaa cgcgcgcagc ttccggcgct
     9601 ggtcgctcat gcgggcgtgc tgttatttgc cgcagggatc gtggtttcca gcgtcagccg
     9661 tcaggagatc agcctgaatt tacagccggg tcagcaggtg acgctggcag gatacacctt
     9721 ccgttttgag cgcctcgatc tgcaagccaa aggcaattac accagcgaaa aagcgatagt
     9781 ggcgctgttt gaccatcagc aacgcattgg tgaattaacg ccggagcggc gtttttatga
     9841 agcacgccgt cagcaaatga tggaaccgtc aattcgctgg aacggcatcc atgactggta
     9901 tgcggtcatg ggtgaaaaaa ccggagcgga tcgttacgct tttcgtttgt atgtacaaag
     9961 cggtgtgcgc tggatctggg gtggaggatt gttgatgatt gcgggcgcat tgttaagcgg
    10021 atggcggggg aagaagcgcg atgaataaag ggcttctcac gctactgctg ttatttacct
    10081 gttttgcgcg cgctcaggtc gtagacacct ggcaattcgc caatccgcaa caacagcaac
    10141 aggcgttaaa tattgccagc cagttacgtt gtccgcagtg ccagaatcaa aacttactcg
    10201 aatccaatgc gccggtggcg gtcagtatgc gccatcaggt ttatagcatg gtgtcggagg
    10261 ggaagaacga agtcgaaatc attggctgga tgaccgaacg ctacggagat tttgttcgct
    10321 ataacccacc gttaacgggt cagacgctgg tgttgtgggc gctgccagtg gtgttgttat
    10381 tgctgatggc actgatcctc tggcgagtga gggctaagcg atgaaacagc ctcaaatacc
    10441 ggtgaaaatg ctgacaacac tcacgatatt gatggtattc ctctgtgtcg gcagttatct
    10501 gttaagccca aaatggcagg cggtacgtgc ggagtatcag cgtcagcgcg atccgctaca
    10561 tcagtttgcc agccagcaaa ccccggaagc gcagcttcag gcattgcagg ataaaatccg
    10621 cgctaatcca caaaacagcg aacaatgggc gttactgggc gagtactatc tgtggcaaaa
    10681 cgattacagc aattcgctgc tggcgtatcg tcaggcgttg caactacgtg gtgagaacgc
    10741 tgaactgtat gcggcgctgg cgacggtgct ttattaccag gccagccagc atatgaccgc
    10801 ccagacttgc gcaatgatcg acaaagccct cgcgctggac tctaacgaaa tcaccgctct
    10861 gatgctgctg gcttccgatg cgtttatgca ggcgaattac gcgcaagcca tcgaactatg
    10921 gcaaaaagtg atggatctca actcaccccg gatcaaccgg acacagctgg tcgagtcgat
    10981 taatatggca aaactgttgc agcggagatc ggattaatca tctggtgatg aagattatgt
    11041 cattgagctg cataaaaaat aatcgaatga acatatgcca aaaataatca ctaatcagta
    11101 ttattgcaga ttaaacaaat aaaaatcttt ccataacaaa tggttattca ttaatcctgc
    11161 cataaactcg cgttattatg cattaatgca gcgaaaagct ctgttgttaa agggttgcgc
    11221 aacataccgc gcaaatgata ctgatcataa gcgttaaaaa aatctacaaa ccaacgcaac
    11281 acaattcatg ccctggcagt atgtcacgtt ctcgcgtttc tgaacgggga acggcgctcc
    11341 attgaggaag ttattctgat gaaaaatata aaattcagcc tggcctggca gattctgttt
    11401 gctatggtgc tgggcattct cctgggaagc tacctgcact accatagcga cagccgcgac
    11461 tggctggtcg tcaatttgct ctctccggcg ggtgatatct tcatccatct gatcaaaatg
    11521 attgttgtgc cgattgtgat ctccacgctg gtggtgggta tcgcgggtgt tggtgatgcc
    11581 aaacagctcg ggcgtattgg cgcgaaaacc attatttact tcgaggtgat caccaccgtc
    11641 gccatcattt tggggatcac tctggcgaac gtcttccagc ccggtgccgg ggtggatatg
    11701 tcgcagctgg cgaccgtcga tatctcgaaa tatcagagca ctacggaagc ggtacaaagc
    11761 agttcccacg gcattatggg cacgattttg tcgctggtgc cgacgaacat tgtggcgtcg
    11821 atggcgaaag gcgaaatgct gccgatcatc tttttctcgg tgctgtttgg tctggggctt
    11881 tcttccctgc ccgcgacgca tcgtgaactg ctggtgaccg tgttccgctc catctctgaa
    11941 accatgttta aagtgactca catggtgatg cgttatgcac cggtgggtgt gtttgcgctg
    12001 attgcggtga cggtggctaa ctttggtttc tcttctctgt ggccactggc gaaactggtg
    12061 ctgctggtgc atttcgccat tctgttcttc gcgctggtag tgctgggaat tgtggcgcgc
    12121 ctgtgcgggt taagcgtctg gatcctgatt cgtattctga aagatgagct gattctggcg
    12181 tactccactg ccagctctga aagcgtactg ccgcgaatta ttgagaagat ggaagcctac
    12241 ggagcaccgg cgtcgatcac cagcttcgtg gtgccgaccg gttactcttt taaccttgat
    12301 ggttcgacgc tgtatcaaag tattgccgct attttcatcg ctcagctgta tggcattgac
    12361 ctgtccatct ggcaggaaat cattctggtg ctgacgctga tggtgacctc gaaagggatt
    12421 gctggcgtgc cgggcgtgtc gtttgtggtg ctgctggcaa cgctgggtag cgtaggtatt
    12481 ccgctggaag gtctggcgtt tattgctggt gttgaccgta tcctcgacat ggcgcgtact
    12541 gcgctgaacg tggtgggtaa tgcgctggcg gtgctggtga ttgccaagtg ggaacacaaa
    12601 tttgaccgca aaaaggcgct ggcttatgag cgtgaagtgc tgggcaaatt tgataaaact
    12661 gcggatcaat aattgaagat tgccggggat atccacccgg caatgtgtga atgcctgatg
    12721 cgacgcttgc cgcgtcttat caggcctacc ttaaccgttg gttaattttt caaactcttc
    12781 acacccggta tcaaaccctt ccatacagct caggttcaac cagtgcagcg ccttttgctt
    12841 gttcttctcg ataaaacctt cttcaccgtt taagaacatc attcccgccc agtactcgga
    12901 ataaccggtt cgggaaattg cagagctgcg tttgaaatac caggttgcct tgtcatcgtc
    12961 ggccttaatg cccacgccgt tggcgtaaat caaaccaagc agcatttggg catcgacggc
    13021 agagtcgttc tccagatctt ccgaggcgtt ttctaacagc gaaatagcct ttggataatc
    13081 cggtttaccc gcttgagtat ttaccagaat atgcgccagc gttacttcac cttctttact
    13141 gcccgcttgc gccgctttct ctgcaagaac ttttgcttgt ggatagtcca gactgaccgg
    13201 attggtgatt ttgatctgcg ccagcagcgc gcaggcatca gcatcaccgc tgtcggcggc
    13261 tttctgtgcc caatattcgg ctttgctcaa atcgccggag ctaaaccagc tgtcggcaag
    13321 aaaatattgt gcgcgtcggt ccccggcctc tgctgccttt aaatactggc tgccaggctc
    13381 ggagtcgttg gcgtgggcaa agaatgtcaa aaacaacatc aatgcgataa tttttttcat
    13441 tttgataata cgattgggta gggagtaacc agtataaaga gttcgaatgg ataaaaaaac
    13501 agcctccgaa aggaggctgt agaaaggact gtattacgcc agtgccgctt cgcgcaggcg
    13561 agttttcaac ttgttgtact cgtcaatcac gtactgctcg gcggcgcgct gatcggcgat
    13621 cggctcgacg cgcacggcgc agtatttgta ttccggcgtt ttcgtaatcg ggcttaagtt
    13681 ttcggtaacc agctcgttac aggcaccaat ccaccactgg taggtcatgt aaatcgcccc
    13741 tttgttcgga cgatcgctga cctgcgcacg ggtgataatt ttgcctttac gcgagtgcac
    13801 ccaaaccaat gcctcatctt caatacccag acgtttggcg tcttcggtat tgatttgtgc
    13861 gtagccaggt tcatcagcca gcgccgccag tgccgcacag ttaccggtca tcgaacggca
    13921 agagtagtga ccaacttcac gcaccgttga cagtaccatc gggtactcgt cggtgagttt
    13981 gtcgattggc gctacccagt cgcaggtgaa gaactgcgcc agaccgttcg gggtatcaaa
    14041 cttctcttta aacagataag aagtcccctg atcggcatct gaagtatcgc ggcaaggcca
    14101 ctgaatgaag cccagttcgc ccattttctc gtaagtcgca ccgtagaaat ccgggcacag
    14161 atgacgcaac tcatcccaga tctcctgggt gttgttgtag tgcatcggat aacccatacg
    14221 ggtggcgatt tcactgatga tttgccagtc cgttttcaga tcccatttcg gttcaactgc
    14281 tttgaagaaa cgctggaagc cacggtcagc cgcagtaaac acgccttcat gctcgcccca
    14341 cgacgttgac ggcaaaatga catccgccgc cgacgcggtt ttggtcataa agatgtcctg
    14401 aacgataacc agttccagat cttcaaaggc tttacgtact gccgacagct ccgcgtcagt
    14461 ttgcagcgga tcttcgccca taatgtacgc ggcacgcact tcgccatgcg ctgcgcggtg
    14521 cggcagctcg ctgatgcgat aaccggtatg agcaggcagg ctttccacgc cccaggcttt
    14581 ggcgaatttc tcgcggttag ccggatcttt cacgtactgg tatcccggat acgtatccgg
    14641 cagcgcgccc atatcgcagg caccctgcac gttgttctga ccacgaaccg ggtttacacc
    14701 cgcatgcggc ttaccgaggt taccagtcag catcgcgagg ctggtcagag aacgcacggt
    14761 ttccacgccc tggtagaact gggttacacc catgccccac aggatggcgg cgcttttcgc
    14821 ctgggcatac atccgtgccg cctgacgaat ctcactggcg ctgacgccgg tgatatcttc
    14881 aaccgactcc ggcgtgtagc cttcaacgat tttacgatac tcttcaaagc cttctgtacg
    14941 ggaagcgacg aacgctttgt cgtacagatt ttcttcaata atgacatggt ccatcgcatt
    15001 caacagcgcg atgttcgagc cgtttttcag tgcaatgtgc atgtcagcaa tgcgcgcggt
    15061 ttcaattttg cgcggatcgc agacgataat tttcgccccg ttacgtttag cgttaattac
    15121 gtgattcgcc acgattgggt gggaatcagc cgggttgtac ccgaaaacga acactaaatc
    15181 ggtattatca atttcgttaa tagcattgct cattgcgcca ttaccgaccg attggtgcag
    15241 acctgcaacc gatgggccgt gtcagacacg agcgcagcag tcaacgttat tggtaccaat
    15301 aacggcgcgc gcaaattttt gcattacata gttggtttcg ttacccgtac cacgcgagga
    15361 gccggtcgtc tggatggcat ccggaccgta cttctctttg atggcgctca ggcgctcggc
    15421 aacgtaattc agtgcctcat cccaggaaac aggttcgagt ttgccgccac gctggcgacg
    15481 gatcatgggg gttttcaggc gcggggtcag gatctgggta tcgttaatga aatcccagcc
    15541 ataataaccc ttcagacaca gggtaccctg gttggttttc ccctgcgctg cctccgcccg
    15601 gacgattttg ccgttatcga cgaccaggtt gattttgcaa cctgatgcgc aataggggca
    15661 aaccgtgacg acttttttca tcggtctcgc tccagttaat caaatcacgc atacgcgctc
    15721 tcgactacag tatgcatctt ttatgccaca ttttatttgg ggtcattccc tgatattacg
    15781 ggcactattt attcaaaact ctgacgaaaa acaggctgtc gtcagttttg acgtgacgaa
    15841 acgaaatacc gcgtgacagc catcacgcgg cagacatttt attttttctc gacgacggga
    15901 gccgcctgat acccgccgcc cagcgatttc atcaactgaa tgctttggat cacccggcgg
    15961 ctgtccagca tcagtaatga catctcttcg gcaagtactg gcaaccgggc ttcagtggcc
    16021 tgtaagcggc tggttaagcc gcgctgatag gcggcctcgg cagcgcgctg ggtgaagcgc
    16081 gttgcttcca cgcgttcagc ctgcatttct cgctcgtcgt tgagcgtttg cagacgcgtg
    16141 ccgttgacgg caacgtcacg caccgcgttc agtactgact ggttgtaacg ttcaatcatc
    16201 atgttgctgg cggcgcgcgt gccttcgaga ttggcattca accgtccacc gtcaaacagc
    16261 ggcaatttca gacccgggat gaagttgaac tggcgactgg tttttttgaa taaggtatcc
    16321 aggtggatgg catcaaggcc gaaaaacgct ttgatatcaa agctcgggta gaacagcgcc
    16381 cgcgcggaat ccacctgatc taatgacgcc tgaacatacc agcgcatggc ttgcagatcc
    16441 gggcgtctgg cgagcaactc ataagagagt gtcgccggaa tgccggtctg gactcgtggt
    16501 aatgccaccg gtttgatctc cggcatatcg ctggcccccg cgccaatcaa tgcacgcaga
    16561 gattctcgcg tttcggtgat ttgccctttg acggcagcaa tttgtttatc gaccgccaga
    16621 atctgtgccc gcgcgccgtg gaaaggcact tgtgcttcca gaccgtgcgc cactttactc
    16681 tggtgcgctt tcaccgcgta atcaatcaca tcgcgagttt gttctaacag atcgagcatc
    16741 tgatagctgg cctgcatact gtaataaagc tgcgctacgc ccgtggtcag cgatagctct
    16801 actgctgcgg tttctgccag cgcggcatta tgcgcgccaa tggcggcggc aaccgctgag
    16861 cgatgcacac cccacaaatc aagatccagt ccggcaaaca aacctactgt ggcttccgta
    16921 tagtacggcc cgtccatacc cagtgcgggc gcatccatcg cataagggct taaaaagccg
    16981 ttcgccgaga cgcgttggcg gttgagcatc cctaacgctg cgacctgtaa ttgtgaaccg
    17041 gcatctaaca aatcggcctg ggactgcgct ttttcttccc gcagtttcgc ttcggcgagg
    17101 gtgtgtgaac cacttagcgt ccgttggatc agcgcatcca gctacgggtc attgagttgt
    17161 ttccaccact gcgcctgcgg ccagccggag ctggcaagat gaatatcatc ggccagtttg
    17221 atttgttccg gtttgagctg ttgatgaggc gcagaatcct tgcgtaccaa ggcacagccg
    17281 gaaatcagcg tcgtgctgcc gagaatgctg cacaacagca gacgtgaaag ttgacgattg
    17341 atcattgcgt ggctccttgt gcctgttccg tggcgggcgt taatgccggg gctgtccagt
    17401 ctggcaagcg ggcgaaaagc tggcagacgt gttgctcctg ttttaataag gtgggcagga
    17461 tggcctgcga ggcgggtgtc tcttcaagcg ttatttgagg agaacggctg agtgcggttg
    17521 ccagaccggc agcgtatttt tccagggcgt cggcaaactg acctgctctg tccggctgta
    17581 gtgcgttatc cagcgcctgc gccgaattcc aggtggcatc ccaggcgtga aggatatcgc
    17641 ggccctgacg aataaccgtt tgcgaacgtt caatcagtaa ggcgcgttct tcgctgtcca
    17701 gttgacgctc cagcgccacg cgttggcaca tctcttcaca ggcattaaac gccgcatgca
    17761 gaccgatacg aatttgcaga taagtgcgca gagccgtgac ttcctgctgg cgtgggatcc
    17821 gcattacttt acttaacata cccagcgcgc cagccagttt ttgcggcagc gtgcgcgctt
    17881 cactttcagg ccagacaaag gtgtaaatca ccgcggacac cacggtacca atgatgatac
    17941 ccagggcgcg atcgcgaatt tccaccaggt cgtacactgg gccaaaaacg ttttcgagcg
    18001 tggcgagcgc gaaggtgacc accatctgtg tgccgatata agaagagcgt tcagagctgg
    18061 tggcaatcca tgcgcccaac aggaaaatcg gtgccagcac aaacagcaat tcgacaatat
    18121 tgtccagcca gggcatgacc agtagcgtga ataacagcgc cagaatcgcg ccgcaaaagg
    18181 ccccgccaaa acgcagcacc atcttctggt acgacgaacc gacatttgga ttagagacga
    18241 tcacgcaggt cagcatacag gtgtgaatgc cttcccagtc cacgccgctg taaaaggtgt
    18301 aacagatcaa acaggcgagc aatgttttta ccgcgtagcg tatatagtct ggattggtaa
    18361 aagcatcggc gaccattgat ggcggtttgg cggcgggcgt tggcggcgta ttcgggttca
    18421 tctggcccag ttgtaacaac gtctggcaga tattctccag gttacattcc cgtgccgcca
    18481 ccgcttcact ttcactgatc cgccagtcgc tttgccagca ctgaccttca gcaacggcat
    18541 gctgcagctt gttgatttct gaggctaatt tttgccgaaa ttcaataatt gcctgagaat
    18601 cagcaaaaga ggtgggatcg tagcgattca gcgtcgagta aatgtaggtt accgttgcca
    18661 cgcagctttg ccaccatgcg ctttgagttc gccagttggc atcgtccgcg agacaaaaga
    18721 cattcagttt ttgcagcgcc agcgcctctc tttcaatccg cgtttcgggt agcggtgcga
    18781 ggctgtctgt caggtgacta atggcatcat caagccgatc attaagcgcc tgatgcattt
    18841 gcgaaatggc acgactggga aaccacagca cgccgattaa cgtcatcagc aaggttggat
    18901 agaggccaac aacgatacac cacagcgtta agcgcacgac cacttccgga tagtcgagca
    18961 tggcggggaa ggtttgcccg taaatggcga caatggcgac agcgaaaaag accagtccca
    19021 gccgatgggt gcgcatcaaa aacatgcggc ccatcaggat cggcccggcg atgatcaatc
    19081 ggatcaacgg ttcgccgtat gaccatttgt agatcaaaaa caggctgccg atctccagca
    19141 ccgtggcaac cacaaacaag atcgccacaa atttggtgta aaacgcgttc gactgaatac
    19201 cgtaaaacag cactgccagc gataacgcca caaaagggat ctcaaaagtc atcgagatca
    19261 gaatcaccag caggcagcct acccagagtt gcacgatctg cggcacgcgg ccgggtcgcc
    19321 gctcgcttaa ctcttcatga aagaacgcca gcagcctgac catcggtaat ggcagggagt
    19381 tgagcgcgct cattattgcg gctcaagatt agcgactgcc gaagcgccga tgcggaacat
    19441 ttccgggtca ggtttatcga ccatgatttt gaccggaaaa cgctgggcaa cgcggaccca
    19501 gttaatagag cgagaaactt tcggcaggcc gcccagcacc aggccgccgt catccggtag
    19561 cacgccgtag ccaatcgaat ccactttacc ctcgaaggtt ttgccgctgt cactcatcag
    19621 gcgaatcgtt gcgggtgtac ctgagcgaat atttttcaga tcggtttcgc ggaagttggc
    19681 gatcacgtac cagtgacgag tgtcgattag ggtaaaaata gggcgcatgg cagaagcaaa
    19741 ttgcccgacg gaggttttga gggaaatgac ccggccatca aacggcgcgc gaacggtcgc
    19801 catttccaga tgcagtttgg tcagggcaat atccgcttcg accgccgcac gttgcgccac
    19861 cagcgcatcg acgccgctga cggcgctggc ggctgactgc gcctgtaaca atacggcatt
    19921 aagatcggct tctgcggcgc gctgcgcagt tcttgcacgg tcaacgtctt ccgctgagac
    19981 aaaaccctct ttcagtaatg gctcggtacg gcgtaatgta tctgtggcct gtttcgcggc
    20041 ggcacgggct ttttctaccg tggcattaat cgagtcggca ccaaactgtt gcgcatcaac
    20101 gctacgctgg gtgagcataa tttgcttatc cagcgccgcg agggaggctt cagctttcgc
    20161 cagattggct tcgtacgggc gcgggtcaat gcggaacagc aaatcgccct gtttgactgc
    20221 ctggttgtcg gtgaccgcca gttctacaat gcggccgctg acttccggca ccacatcaat
    20281 ggtatctgct gacgcgtaag cgtcattagt tgatggcgca ctgtctacgc gccagataac
    20341 gaaaacaagg gcaaccagcg ccaatgcaac cactaacaga gcagggaatt tactgcgagg
    20401 agcttttttc ggcgtacttt ccatgagcgc gtctcaaata gattagaaaa atgccagcca
    20461 gaacagcatg gcgtagagag caaaaagggc ggtataaata attccgacaa atgccagatt
    20521 gatatttgcc cgctgaataa ctcgcctcgt gatgagcatc aaaataaggc tggcaatggc
    20581 gcagaaaaac cagctgggat aataagcacc gatcaccggg attgccggag ataatgagca
    20641 tcccgaaacc atgaagacgg gaattatcca ggctgttttt ttgagttgca tcgccatgcg
    20701 agagagaact gttggcatat taaatacatc ctgtctgctg cgaacaatat ctgtagtgaa
    20761 tatcaatatc cattgaaggc gtcagtatgg ctattttctc gatgaataaa aatagcttta
    20821 ttcagtcacc gcattcataa cttatatatt ttatttatta attggct
//
LOCUS       DAZPEP010000061        20427 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZPEP010000061 DAZPEP010000000
VERSION     DAZPEP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.061"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="ACHUT9_002233"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="ACHUT9_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFR1348700.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="ACHUT9_002234"
     CDS             433..621
                     /locus_tag="ACHUT9_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFR1348701.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="ACHUT9_002235"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="ACHUT9_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFR1348702.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="ACHUT9_002236"
     CDS             816..989
                     /locus_tag="ACHUT9_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFR1348703.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="ACHUT9_002237"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="ACHUT9_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUT9_002238"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="ACHUT9_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFR1348704.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFATQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="ACHUT9_002239"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="ACHUT9_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFR1348705.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUT9_002240"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="ACHUT9_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFR1348706.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="ACHUT9_002241"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="ACHUT9_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFR1348707.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNESDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="ACHUT9_002242"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="ACHUT9_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10407..10952
                     /gene="torD"
                     /locus_tag="ACHUT9_002243"
     CDS             10407..10952
                     /gene="torD"
                     /locus_tag="ACHUT9_002243"
                     /inference="COORDINATES: protein motif:HMM:NF003442.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFR1348708.1"
                     /translation="MFSRELDDEQLTQISSAQMAEWFSLLKSEPPLTAAVNALENRIA
                     ALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKRLLVEAGMETSGNFN
                     ESADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTALRQWLPEFAARCRQYDSF
                     GFYAALSQLLLVLVECDHQNR"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUT9_002244"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="ACHUT9_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFR1348709.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUT9_002245"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="ACHUT9_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFR1348710.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            12531..13677
                     /locus_tag="ACHUT9_002246"
                     /pseudo
     CDS             12531..13677
                     /locus_tag="ACHUT9_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13971..15212
                     /gene="agp"
                     /locus_tag="ACHUT9_002247"
     CDS             13971..15212
                     /gene="agp"
                     /locus_tag="ACHUT9_002247"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFR1348711.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(15250..15477)
                     /locus_tag="ACHUT9_002248"
     CDS             complement(15250..15477)
                     /locus_tag="ACHUT9_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFR1348712.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="ACHUT9_002249"
     CDS             complement(15498..16094)
                     /gene="wrbA"
                     /locus_tag="ACHUT9_002249"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFR1348713.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            16467..16640
                     /locus_tag="ACHUT9_002250"
     CDS             16467..16640
                     /locus_tag="ACHUT9_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFR1348714.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="ACHUT9_002251"
     CDS             complement(16898..18226)
                     /gene="rutG"
                     /locus_tag="ACHUT9_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFR1348715.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="ACHUT9_002252"
     CDS             complement(18247..18741)
                     /gene="rutF"
                     /locus_tag="ACHUT9_002252"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFR1348716.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(18752..19342)
                     /locus_tag="ACHUT9_002253"
     CDS             complement(18752..19342)
                     /locus_tag="ACHUT9_002253"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFR1348717.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="ACHUT9_002254"
     CDS             complement(19352..20152)
                     /gene="rutD"
                     /locus_tag="ACHUT9_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFR1348718.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPC
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(20160..>20427)
                     /gene="rutC"
                     /locus_tag="ACHUT9_002255"
     CDS             complement(20160..>20427)
                     /gene="rutC"
                     /locus_tag="ACHUT9_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1348719.1"
                     /translation="NVLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWK
                     NYAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         5106 a         5201 c         5242 g         4878 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggc gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcgag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gtggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgagtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatctc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtttaa ctagggcgtc attatttatt aaattttata gacgctatat atgggtagta
    12541 atatacatgg aattagttgc actgcaaata attatttgaa acaggcctgg aacgatataa
    12601 aaaatgagta cgaaaaaaat caaacatatt caatcacgct ttttgaaaac acactggtgt
    12661 gttttatgcg gttatacaat gaactcagac gtaaagtaaa tgaagaggat actccatgtc
    12721 tggaatgtga atcactagaa aaagaatttg aggaaatgca gaatgataat gatctatcat
    12781 tatttatgag aacattgcgt actaataata cacaaattta ttcaggggtt tcaggaggta
    12841 ttacatatac tatacaatat gttcaaaatg ttgatattgt tagagtgtcc ttgccgggca
    12901 gaggttcaga gtctatcaca gattttaaag gttattattg gtatggcttt atggaatata
    12961 ttgaaaacat tactgcgtgt gatgatattt tttctgagta ttgtttagat gatgaaaata
    13021 tgagtatcca gccagaacag ataaatacgc cgggaatatc tgatttagat actggtttta
    13081 aaagaacatg agtattcatg ggcgctgggc ggccataaga cgcaaaagca agtatacgaa
    13141 gagtttatta aaattgttga taaacaaacg atcgaggctc ttgttgatac ggcattctat
    13201 aatctcaggg aagatgtaaa gacgttattt ggcgttaatc tacaatctga caacaaaatt
    13261 caggggcagg gtggtttttt atcatggagt tttttgtctt ttaaaaaaga atttattgat
    13321 agttgcttga atgacaaaaa atgcatactg catttacccg aatttatatt taatgataac
    13381 aaggcacgcc ttgttttaga caccgaccca gagcagaagg ttaatgaggt gaaaaatttc
    13441 ctcactgctc tttcagatag catttgctca ttatttattg tttacagcaa tgtggcatca
    13501 atctccttag ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg gtatttaata
    13561 aacactggca atcattatga cgtttacctc cctcctgaac tttttgctca ggcttacgag
    13621 ttaaacaata aggaaaggaa tgcgcaaatt gattttttaa ctcgttatgc aatttaatga
    13681 caaaggcatg tgctaaaaac cattgttatt agcctcactt ctcttattat tggtactatt
    13741 gcctctgtat tagtaacgcc gctgatattc tgtttagcca caggtgcaat tatcagcggc
    13801 gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg tttttgaaga
    13861 tatatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc ggaaaaaagt
    13921 catatttctg tcacactctt taatgattga taacaaaaga ggtgccagga atgaacaaaa
    13981 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
    14041 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
    14101 cgccgctggc gaacaatggc agtgtgctgg agcagtcgac gccgaataaa tggccagaat
    14161 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
    14221 attacatgcg tgaatggctg gcagagcagg ggatggtgaa atcgggggaa tgcccgccgc
    14281 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
    14341 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
    14401 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
    14461 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
    14521 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
    14581 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
    14641 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
    14701 cgatggatca ggtggcctgg ggagaaatca aatctgacca gcagtggaag gtgttgtcga
    14761 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
    14821 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
    14881 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
    14941 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
    15001 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
    15061 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
    15121 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
    15181 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
    15241 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
    15301 atcggcctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
    15361 tgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
    15421 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
    15481 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
    15541 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
    15601 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg aaacgtcaaa taattcctgc
    15661 gctgcgtagc caatggggac aatcaccatg ccgtgatgcg caagggtcgt ccaggttgaa
    15721 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
    15781 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
    15841 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
    15901 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
    15961 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
    16021 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcacc
    16081 agaactttag ccatttctaa ccactcctcg tgttatctct attccgtagc gattcgctac
    16141 cacttattta aagataagac gtccttttca gagtgcaaat ttcacaacca cttatttgat
    16201 ttataccaac tttcacaagc acgtaatttt gtcgcaaaat gacacatttt tatctcatcg
    16261 cgttttttta atcataagag cggcttatgg ataattattg gagatgatat ctattctcgc
    16321 taagaagctg ttgcaggata ttaccaaacg cgggtctacc cgcgtcagtt cactaagctt
    16381 agtcccacgt agcgaaaata tggcagccgc catacgccgc gttaattcta tgcaatatga
    16441 tgtctatacc cagacggagg tcagtaatgg caaaccatcg aggcggttcc ggcaattttg
    16501 cagaagaccg cgaaagagca tcagaagcag gtaaaaaagg tggacagcac agcgggggta
    16561 atttcaagaa tgacccgcag cgcgcatctg aagcaggtaa aaaaggtggc aagagcagtc
    16621 acggcaaaag cgacaactag ccgggctaat caatgacgaa tgcatttttg tctgtagctc
    16681 gtcaaaaagc catcaccgcc ggttacccgg tggttgatac tgatgacaaa tgtaagcttg
    16741 cctgatgcgc gatgcttatc aggcctacca gaagattgca atatattgaa tttgcactgt
    16801 tttgtaggcc ggataaggcg tttacgccgc atccggcatg aacaatgcgt acgttgtcaa
    16861 caatctgcac cgccgggtaa ccccggcggt tttctgttta tggctcctga tgaacaactt
    16921 ctggcggtgg aacgtcaacc aaccttctac ttaacaacgc attgagtaaa atcgcgccaa
    16981 aggtggctgt accgatccct cccaacgtaa aaccgcccag cgtgagagca aaatcacccg
    17041 cgcccagcac taaggttact gcgaccataa tcaaattacc gttctggctt aaatcgacac
    17101 ggttttgtac ccagatcctt gcgcctgcga cggcaatcag cccgaacaca acaattgatg
    17161 caccaccaat caccgcggcc ggaatggtat ggatcagcgc gccaaatttc ggcgaaaagc
    17221 ccaacagcat ggcgatgacg gcagcagcaa caaacaccag cgtcgagtag acttttgtca
    17281 cggccatcac accgatattt tcagcatagg tggtgacgcc gctaccgccg acagagccgg
    17341 aaagcatcgt tgccagacca tcaccgacga atgctcgccc catatacggg tccatattgc
    17401 gtccggtcat cccggcgacc gccttgagat gacctaagtt ttccgccacc agaatcaccg
    17461 ccacgggcgc aatcagcatc attgcctgac tattaaaagc aggagtggaa aaatgtggca
    17521 aaccgaacca ggcagcatgg ctgacgagag taaaatcgac ggcttttccc agccctaaaa
    17581 cgttggtcat cacgccatac agcagacagg cgacaattaa acctacgaga atcaataacc
    17641 gctggatcat gccacgggta aacaccgcca ccagcccaat acacagcacc gtcattaccg
    17701 ccatccagct atcaaaggcc gaagccgata cacttttcac tgcgataggc gctaagttca
    17761 ggccaatcgc catcaccacc gcacccgtca ccaccggcgg cattagtcgt tcaatccagc
    17821 gtgtaccaat tttcatcacc accaggccaa tgacggtata aaccagccca caggcgataa
    17881 tcccgcccag tgcgatgctg atattcgggt taatgccctg accgttaaag cccgtcgcgg
    17941 caatcaccac gccgacaaaa gccgcgctgg agccgagata actggggacg cgcccgccgg
    18001 taataaagaa aaacagtaac gtgccgatcc ccgacattaa aatggaaaga ttgggatcca
    18061 gccccatcag aatcggcatt aacaccgtcg cgccaaacat cgccaccgcg tgttgaacgc
    18121 ccattactgc cgtctgagca aacggcaatc gttcatccgg cgcgaccacg ccgctctctg
    18181 tagaggtcga ttttaactgc cagtgaggaa aaccgaacat tgccatcagc tgtctcctta
    18241 aggaggttaa caagcagggc gcatcagcgc gtgataactg cgatcgaacc acaccagccc
    18301 gtagggtgtg gtgtgacgat gaatcgcttc gatggcgcaa aacagaatgt cgtgggtgcc
    18361 gacgctcacc acctggctga tgcggcagtc aaacgaaacc agagcctctt ccagttgcgg
    18421 gcatccggtc acacccgtct gccagcgggc ggcggcaaag cggtgttcca tgggcgtttt
    18481 gccgccaaaa agatttgaaa gcggctcctg cccggcgctc agtgtattta cacacagcgt
    18541 tcgattttca ttgaataccg gccagacgga cgccccacga ttcaggcaca ccagtaatgt
    18601 gggcggcgtg tcggtcacgc tgcagacggc gctggcggtg aacccggcgc gcccggatgg
    18661 accgtccgtg gtgatgatat tgaccgccgc gcccatacag gacatcgcat cgcgaaaggt
    18721 ttgttgatca actatgttca tagtttgctc cttacaacag cccgcaggct tcttcaaagg
    18781 acagacgtgg caggcgcgca aaaagtttgc tgctatcgcc atagccgata ttaatcagca
    18841 gattgctctt cagcgtgctg cccgcaaaaa aggcgtcgtc aacgtgttga cggtcaaaac
    18901 ccgacatcgg gccggtatcc agtcccagcg cacggcaagc gacgatcaga taggccgcct
    18961 gcatggagct gttgcgaaac gctgtttctt cggcaagttg tgggctggag gtaaaccaac
    19021 tgcgggcatc accgtgggga aacagtagtg gtaaccgttc ataaaattca ctgtcccagg
    19081 caacgatagc ggtgacgggc gcggtcaggg ttttttgtag attgccgctg gaaagcgccg
    19141 ggtgcagacg ttcttttcct tctacagtgc ggataaacac gatccgtgcc ggagaacagt
    19201 tagctgatgt cggcccccat tttatcgggg cataaagctc ccgtaacgtc tcatcgctga
    19261 cgggtgtctc ccgccagccg ttgtgagtgc gggcatcggt gaacagggtg ctaagcgcac
    19321 ctgggctaac ggcttcgttc atagcaattc cttacagggc ggcttcacgg tgatgtaaca
    19381 ggctggcaag cccgttgagt aacagagcat taaacgtttc ggggtcggtc acgttgcagg
    19441 cgtgtccgcc atagcgcatc accattttct ggctatcggg cagggcggca tgaagctcac
    19501 tggaacatgc tgatggcacc agcagatcat cactggcgca gatgatttgc accgggcagc
    19561 ggatgcgatc cgcatggtga ctaaagtcag cgcgtttgag ggcgttaagt cgacgcagta
    19621 aattattttt gccctgaaaa tgcgccagtg ccagcgcgtc ttctgcctca aggcaaggtg
    19681 cgcgggccgc catccagtcg gcgggataga ggaacaacgg ctgcgcttcc acccatgcct
    19741 gcgcgccgcc gctatacagt aatcgttcgc gaacctgaaa acagcggcgc gtatgggcgt
    19801 ttattcgtag ccagccgtta acgctgatca gcacagttac cgacgcggga taatccagcg
    19861 ccagctgcat tcccaccagc gcaccgagcg catggccgac caccgcgtaa tgctcaatcc
    19921 ctgcggctac cagcgcctga tgcagttccg ctgccatctg ggcgatactg taatcttctg
    19981 ccagcgtgtc gggattattg ccggtgccgc gctggtcgta acagactacc tgatactcct
    20041 gctccagcac cgccagttgc ggtaaccagt aactgccgct accgccaaga cccgaaatca
    20101 acaccactac gggcgcatca gcataagggg gaggtgagag tgaaagtttc atcgcggcct
    20161 cacttggcga tatgcgcaat tgtggcgatt tccaccagcg cgtcaggttt taccagtccg
    20221 cactgaatgc agaatcgcgc cggtttatca cccggaaaaa actcggcgta gatttcgtta
    20281 atcgcggcgt aatttttcca gtcggtaata aagatgctgt tgaaggtcac atccgccatc
    20341 gtgccacccg ccgtctcgat caccttgcgg atagtttcca gaacgtggcg ggtttgcgcc
    20401 tttgggtcat cggcaaacag cacgtta
//
LOCUS       DAZPEP010000062        20381 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZPEP010000062 DAZPEP010000000
VERSION     DAZPEP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20381
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.062"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(216..1028)
                     /locus_tag="ACHUT9_002256"
     CDS             complement(216..1028)
                     /locus_tag="ACHUT9_002256"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFR1348720.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            1290..2051
                     /gene="udp"
                     /locus_tag="ACHUT9_002257"
     CDS             1290..2051
                     /gene="udp"
                     /locus_tag="ACHUT9_002257"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFR1348721.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            2192..3619
                     /gene="rmuC"
                     /locus_tag="ACHUT9_002258"
     CDS             2192..3619
                     /gene="rmuC"
                     /locus_tag="ACHUT9_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFR1348722.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            3714..4469
                     /gene="ubiE"
                     /locus_tag="ACHUT9_002259"
     CDS             3714..4469
                     /gene="ubiE"
                     /locus_tag="ACHUT9_002259"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFR1348723.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            4483..5088
                     /gene="ubiJ"
                     /locus_tag="ACHUT9_002260"
     CDS             4483..5088
                     /gene="ubiJ"
                     /locus_tag="ACHUT9_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFR1348724.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            5085..6725
                     /gene="ubiB"
                     /locus_tag="ACHUT9_002261"
     CDS             5085..6725
                     /gene="ubiB"
                     /locus_tag="ACHUT9_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFR1348725.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            6804..7073
                     /gene="tatA"
                     /locus_tag="ACHUT9_002262"
     CDS             6804..7073
                     /gene="tatA"
                     /locus_tag="ACHUT9_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFR1348726.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            7077..7592
                     /gene="tatB"
                     /locus_tag="ACHUT9_002263"
     CDS             7077..7592
                     /gene="tatB"
                     /locus_tag="ACHUT9_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFR1348727.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            7595..8371
                     /gene="tatC"
                     /locus_tag="ACHUT9_002264"
     CDS             7595..8371
                     /gene="tatC"
                     /locus_tag="ACHUT9_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFR1348728.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            8413..9195
                     /gene="tatD"
                     /locus_tag="ACHUT9_002265"
     CDS             8413..9195
                     /gene="tatD"
                     /locus_tag="ACHUT9_002265"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFR1348729.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(9192..9680)
                     /gene="rfaH"
                     /locus_tag="ACHUT9_002266"
     CDS             complement(9192..9680)
                     /gene="rfaH"
                     /locus_tag="ACHUT9_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFR1348730.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            9847..11340
                     /gene="ubiD"
                     /locus_tag="ACHUT9_002267"
     CDS             9847..11340
                     /gene="ubiD"
                     /locus_tag="ACHUT9_002267"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFR1348731.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            11387..12088
                     /gene="fre"
                     /locus_tag="ACHUT9_002268"
     CDS             11387..12088
                     /gene="fre"
                     /locus_tag="ACHUT9_002268"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFR1348732.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(12278..13441)
                     /gene="fadA"
                     /locus_tag="ACHUT9_002269"
     CDS             complement(12278..13441)
                     /gene="fadA"
                     /locus_tag="ACHUT9_002269"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFR1348733.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(13451..15640)
                     /gene="fadB"
                     /locus_tag="ACHUT9_002270"
     CDS             complement(13451..15640)
                     /gene="fadB"
                     /locus_tag="ACHUT9_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFR1348734.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLSMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            15830..17161
                     /gene="pepQ"
                     /locus_tag="ACHUT9_002271"
     CDS             15830..17161
                     /gene="pepQ"
                     /locus_tag="ACHUT9_002271"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFR1348735.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            17161..17775
                     /locus_tag="ACHUT9_002272"
     CDS             17161..17775
                     /locus_tag="ACHUT9_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFR1348736.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            17814..19265
                     /gene="trkH"
                     /locus_tag="ACHUT9_002273"
     CDS             17814..19265
                     /gene="trkH"
                     /locus_tag="ACHUT9_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFR1348737.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            19277..19822
                     /gene="hemG"
                     /locus_tag="ACHUT9_002274"
     CDS             19277..19822
                     /gene="hemG"
                     /locus_tag="ACHUT9_002274"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFR1348738.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            20200..>20381
                     /locus_tag="ACHUT9_002275"
     rRNA            20200..>20381
                     /locus_tag="ACHUT9_002275"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         4776 a         5149 c         5550 g         4906 t
ORIGIN      
        1 aattaatgtt tgtttgtgaa tgggagttga tggtaaatct ctaaggtgtc ttccattgaa
       61 gagactcatt tatgactatg acttgccgac tttcttaagg tcaaatagcg gagtcgtaat
      121 ctccaatcaa ttcatactga ttattttttg ggggacagag atggcagcaa aacgcactcg
      181 tttactcacc atctcaaata tagactaaga ggaaactaac tccgcttctc tccatactgc
      241 ttaaaccatt ccagcatacg ctgccagcca tctttcgcag attcggcatg atagctcggg
      301 cgataatcag cgttgaatgc atgcccggca tccgggtaca cgataatctc tgctttcgca
      361 ttagcagccc gcagcgcctg gcgcatggtt tcaacgctct cctgcggaat gctgttatcc
      421 tgaccgccat ataagccgag aatcggcgcg ttaagatcgg ttgcgatatc aacaggttgt
      481 ttcggtgaat tcagcgactt gtcgcccgtc agtttgccgt accacgccac tgcagctttt
      541 agctgtggat tatgcgcggc atacagccag gtgatacgtc caccccagca gaatccggtg
      601 atcattaaac gatgaacatc gccgccgttg cgggaagccc aactggcggc atgatcgaga
      661 tcggccagca cctgcgagtc aggcactttt gctaccagac cgctaagcaa cgtggggata
      721 tcggcaaaat cattcggatc gccttcgcgg aagtaaagtt caggtgcgat agccagatac
      781 ccctccagcg ccagacggcg acaaatgtcg cggatatgtt catgcacgcc aaaaatttcc
      841 tgcactacaa tgaccactgg cagtgggcca tcgctttgct ttggtctggc atggtaagca
      901 ggcatgttat ccccttgtga agggatagag gtgaagcccg ccacaatcgc gtcgtccagg
      961 gtctgaacga tggtcgaagc gagaggcgat gcagcaggtg caaatccaga ttgttgtgtt
     1021 gttgccatgg tattctccgt acccttataa aaatgttgcg caatgttaac tatagtcagc
     1081 atgcaacaaa tcacattgcc tgaatcggct catcttttat gcagtcctgc agaatgaaga
     1141 gtgatttatg tgatttgaat cacttttggt gggtaaattt atgcaacgca tttgcgtcat
     1201 ggtgatgagt atcacgaaaa aatgttaaac ccttcggtaa agtgtctttt tgcttcttct
     1261 gactaaaccg attcacagag gagttgtata tgtccaagtc tgatgttttt catctcggcc
     1321 tcactaaaaa cgatttacaa ggggctacgc ttgccatcgt ccctggcgac ccggatcgtg
     1381 tggaaaagat cgccgcgctg atggataagc cggttaagct ggcatctcac cgcgaattca
     1441 ctacctggcg tgcagagctg gatggtaaac ctgttatcgt ctgctctacc ggtatcggcg
     1501 gcccgtctac ctctattgct gttgaagagc tggcacagct gggcattcgc accttcctgc
     1561 gtatcggtac aacgggcgct attcagccgc atattaatgt gggtgatgtc ctggttacca
     1621 cggcgtctgt ccgtctggat ggcgcgagcc tgcacttcgc accgctggaa ttcccggctg
     1681 tcgctgattt cgaatgtacg actgcgctgg ttgaagctgc gaaatccatt ggcgcgacaa
     1741 ctcacgttgg cgtgacagct tcttctgata ccttctaccc aggtcaggaa cgttacgata
     1801 cttactctgg tcgcgtagtt cgtcacttta aaggttctat ggaagagtgg caggcgatgg
     1861 gcataatgaa ctatgaaatg gaatctgcaa ccctgctgac catgtgtgca agtcagggcc
     1921 tgcgtgccgg tatggtagcg ggtgttatcg ttaaccgcac ccagcaagag atcccgaatg
     1981 ctgagacgat gaaacaaact gaaagccaag cggtgaaaat cgtggtggaa gcggcgcgtc
     2041 gtctgctgta attctcttct cctgtctgaa ggccgacgcg ttcggccttt tgtatttttg
     2101 cgtagcgcct cgcaggaaat gcctttccaa ctggacgttt gtacagcaca attctatttt
     2161 gtgcgggtaa gttgttgcgt caggaggcgt tgtggatttc tcaatcatgg tttacgcagt
     2221 tattgcgttg gtgggtgtgg caattggctg gctgtttgcc agttatcaac atgcgcagca
     2281 aaaagccgag caattagctg aacgtgaaga gatggtcgcg gagttaagcg cggcaaaaca
     2341 acaaattacc caaagcgagc actggcgtgc agagtgcgag ttactcaata acgaagtgcg
     2401 cagcctgcaa agtattaaca cctctctgga ggccgatctg cgtgaagtaa ccacgcggat
     2461 ggaagccgca cagcaacatg ctgacgataa aattcgccag atgattaaca gcgagcagcg
     2521 cctcagtgag cagtttgaaa acctcgccaa ccgtattttt gagcacagca atcgccgggt
     2581 tgatgagcaa aaccgtcaga gtctgaacag cctgttgtcg ccgctacgtg aacaactgga
     2641 cggtttccgc cgtcaggttc aggacagctt cggtaaagaa gcacaagaac gccataccct
     2701 gacccacgaa attcgcaatc tccagcaact caacgcgcaa atggcccagg aagcgatcaa
     2761 cctgacgcgc gcgctgaaag gcgacaataa aacccagggc aactggggcg aggtagtatt
     2821 gacgcgggtg ctggaggctt ccggtctgcg tgaagggtat gaatatgaaa cccaggtcag
     2881 catcgaaaat gacgcccgct cgcggatgca gccggatgtc atcgtgcggc tgccgcaggg
     2941 aaaagatgtg gtgatcgacg ccaaaatgac gctggtcgcc tatgaacgct attttaacgc
     3001 cgaagacgac tacacccgcg aaagcgcgct acaggaacat atcgcgtcgg tgcgtaacca
     3061 tatccgtttg ctgggacgca aagattatca acagctgccg gggctgcgaa ctctggatta
     3121 cgtgctgatg tttattcccg ttgaacccgc ttttttactg gcgcttgacc gccagccgga
     3181 gctgatcacc gaagcgttga aaaacaatat catgctggtt agcccgacta cgctgctggt
     3241 ggcgttgcgt actattgcca acctgtggcg ttatgagcat caaagccgca acgcccagca
     3301 aatcgccgat cgtgccagca agctgtacga caagatgcgt ttgttcatcg atgacatgtc
     3361 cgcgattggt caaagtctcg acaaagcgca ggataactac cgccaggcaa tgaaaaaact
     3421 ctcttcaggt cgcggaaatg tgctggcgca ggcagaagcg tttcgcggtt taggagtaga
     3481 aattaaacgc gagattaatc cggatttggc tgaacaggcg gtgagccaga atgaagagta
     3541 tcgacttcgg tcggttccgg agcagccgaa tgatgaagct tatcaacgcg atgatgaata
     3601 taatcagcag tcgcgctagc ccattagggg tagttaagcc gggtagaaat ctagggcatc
     3661 gacgcccaat ctgttacact tctggaacaa ttttttgatg agcaggcatt gagatggtgg
     3721 ataagtcaca agaaacgacg cactttggtt ttcagaccgt cgcgaaggaa caaaaagcgg
     3781 atatggtcgc ccacgttttc cattccgtgg catcaaaata cgatgtcatg aatgatttga
     3841 tgtcatttgg tattcatcgt ttgtggaagc gattcacgat tgattgcagc ggcgtacgcc
     3901 gtgggcagac cgtgctggat ctggctggtg gcaccggcga cctgacagcg aaattctccc
     3961 gcctggtcgg agaaactggc aaagtggtcc ttgctgatat caatgaatcc atgctcaaaa
     4021 tgggacgcga gaagctgcgt aatatcggtg tgattggcaa cgttgagtat gttcaggcga
     4081 acgctgaggc gctgccgttc ccggataaca cctttgattg catcaccatt tcgtttggtc
     4141 tgcgtaacgt caccgacaaa gataaagcac tgcgttcaat gtatcgcgtg ctgaaacccg
     4201 gcggccgcct gctggtgctt gagttctcga agccaattat cgagccgctg agcaaagcct
     4261 atgatgcata ctccttccat gtgctgccgc gtattggctc actggtcgcg aacgacgccg
     4321 acagctaccg ttatctggca gaatccatcc gtatgcatcc cgatcaggat accctgaaag
     4381 ccatgatgca ggatgccgga ttcgaaagtg tcgactacta caatctgacg gcaggggttg
     4441 tggcgctgca tcgtggttat aagttctgac aggagaccgg aaatgccttt taaaccttta
     4501 gtgacggcag gaattgaaag tctgctcaac accttcctgt atcgctcacc cgcgctgaaa
     4561 acagcccgct cgcgtctgct gggtaaagta ttgcgcgtgg aggtaaaagg cttttcgacg
     4621 tcattgattc tggtgttcag cgaacgccag gttgatgtac tgggcgaatg ggcaggcgat
     4681 gctgactgca ccgttatcgc ctacgccagt gtgttgccga aacttcgcga tcgccagcag
     4741 cttaccgcac tgattcgcag tggtgagctg gaagtgcagg gcgatattca ggtggtgcaa
     4801 aacttcgttg cgctggcaga tctggcagag ttcgaccctg cggaactgct ggccccttat
     4861 accggtgata tcgccgctga aggaatcagc aaagccatgc gcggaggcgc aaagttcctg
     4921 catcacggca ttaagcgcca gcaacgttat gtggcggaag ccattactga agagtggcgt
     4981 atggcacccg gtccgcttga agtggcctgg tttgcggaag agacggctgc cgtcgagcgt
     5041 gctgttgatg ccctgaccaa acggctggaa aaactggagg ctaaatgacg ccaggtgaag
     5101 tacggcgcct atatttcatc attcgcactt ttttaagcta cggacttgat gaactgatcc
     5161 ccaaaatgcg tatcaccctg ccgctacggc tatggcgata ctcattattc tggatgccaa
     5221 atcggcataa agacaaactt ttaggtgagc gactacgact ggccctgcaa gaactggggc
     5281 cggtttggat caagttcggg caaatgttat caacccgccg cgatcttttt ccaccgcata
     5341 ttgccgatca gctggcgtta ttgcaggaca aagttgctcc gtttgatggc aagctggcga
     5401 agcagcagat tgaagctgca atgggcggct tgccggtaga agcgtggttt gacgattttg
     5461 aaatcaagcc actggcttct gcttctatcg cccaggttca taccgcgcga ttgaaatcga
     5521 atggtaaaga ggtggtgatt aaagtcatcc gcccggatat tttgccggtt attaaagcgg
     5581 atctgaaact tatctaccgt ctggctcgct gggtgccgcg tttgctgccg gatggtcgcc
     5641 gtctgcgccc aaccgaagtg gtgcgcgagt acgaaaagac cttgattgat gaactgaatt
     5701 tgctgcggga atctgccaac gccattcagc ttcggcgaaa ttttgaagac agcccgatgc
     5761 tctacatccc ggaagtttac cctgactatt gtagtgaagg gatgatggtg atggagcgca
     5821 tttacggcat tccggtgtct gatgttgcga cgctggagaa aaatggcacc aacatgaaat
     5881 tgctggcgga acgtggcgtg caggtgttct tcactcaggt ctttcgcgac agctttttcc
     5941 atgccgatat gcaccctggc aacattttcg taagctatga acacccggaa aacccgaaat
     6001 atatcggcat tgattgcggg attgttggct cgctaaacaa agaagataaa cgctatctgg
     6061 cagaaaactt tatcgccttc tttaatcgcg actatcgcaa agtggcagag ctacacgtcg
     6121 attctggctg ggtgccacca gataccaacg ttgaagagtt cgaatttgcc attcgtacgg
     6181 tctgtgaacc tatctttgag aaaccgctgg ccgaaatttc gtttggacat gtactgttaa
     6241 atctgtttaa tacggcgcgt cgcttcaata tggaagtgca gccgcaactg gtgttactcc
     6301 agaaaaccct gctctatgtc gaaggggtag gacgccagct ttatccgcag ctcgatttat
     6361 ggaaaacagc gaagcctttc ctggagtcgt ggattaaaga tcaggtcggt attcctgccc
     6421 tggtgagagc atttaaagaa aaagcgccgt tctgggtcga aaaaatgcca gaactgcctg
     6481 aattggttta cgacagtttg cgccagggca agtatttaca gcacagtgtt gataagattg
     6541 cccgcgagct tcagtcaaat catgtacgtc agggacaatc gcgttatttt ctcggaattg
     6601 gcgctacgtt agtattaagt ggcacattct tgttggtcag ccgacctgaa tgggggctga
     6661 tgcccggctg gttaatggca ggtggtctga tcgcctggtt tgtcggttgg cgcaaaacac
     6721 gctgattttt tcatcgctca aggcgggtcg tgtaacgtat aatgcggcct tgtttaatca
     6781 tcatctacca cagaggaaca tgtatgggtg gtatcagtat ttggcagtta ttgattattg
     6841 ccgtcatcgt tgtactgctt tttggcacca aaaagctcgg ctccatcggt tccgatcttg
     6901 gtgcgtcgat caaaggcttt aaaaaagcaa tgagcgatga tgaaccaaag caggataaaa
     6961 ccagtcagga tgctgatttt actgcgaaaa ctatcgccga taagcaggcg gatacgaatc
     7021 aggaacaggc taaaacagaa gacgcgaagc gccacgataa agagcaggtg taatccgtgt
     7081 ttgatatcgg ttttagcgaa ctgctattgg tgttcatcat cggcctcgtc gttctggggc
     7141 cgcaacgact gcctgtggcg gtaaaaacgg tagcgggctg gattcgcgcg ttgcgttcac
     7201 tggcgacaac ggtgcagaac gaactgaccc aggagttaaa actccaggag tttcaggaca
     7261 gtctgaaaaa ggttgaaaag gcgagcctca ctaacctgac gcccgaactg aaagcgtcga
     7321 tggatgaact acgccaggcc gcggagtcga tgaagcgttc ctacgttgca aacgatcctg
     7381 aaaaggcgag cgatgaagcg cacaccatcc ataacccggt ggtgaaagat aatgaagctg
     7441 cgcatgaggg cgtaacgcct gccgctgcac aaacgcaggc cagttcgccg gaacagaagc
     7501 cagaaaccac gccagagccg gtggtaaaac ctgctgcgga cgctgaaccg aaaaccgctg
     7561 caccttcccc ttcgtcgagt gataaaccgt aaacatgtct gtagaagata ctcaaccgct
     7621 tatcacgcat ctaattgagc tgcgtaagcg tctgctgaac tgcattatcg cggtgatcgt
     7681 gatattcctg tgtctggtct atttcgccaa tgacatctat cacctggtat ccgcgccatt
     7741 gatcaagcag ttgccgcaag gttcaacgat gatcgccacc gacgtggcct cgccgttctt
     7801 tacgccgatc aagctgacct ttatggtgtc gctgattctg tcagcgccgg tgattctcta
     7861 tcaggtgtgg gcatttatcg ccccagcgct gtataagcat gaacgtcgcc tggtggtgcc
     7921 gctgctggtt tccagctctc tgctgtttta tatcggcatg gcattcgcct actttgtggt
     7981 ctttccgctg gcatttggct tccttgccaa taccgcgccg gaaggggtgc aggtatccac
     8041 cgacatcgcc agctatttaa gcttcgttat ggcgctgttt atggcgtttg gtgtctcctt
     8101 tgaagtgccg gtggcaattg tgctgctgtg ctggatgggg attacctcgc cagaagactt
     8161 acgcaaaaaa cgcccgtatg tgctggttgg tgcattcgtt gtcgggatgt tgctgacgcc
     8221 gccggatgtc ttctcgcaaa cgctattggc gatcccgatg tactgtctgt ttgaaatcgg
     8281 tgtcttcttc tcacgctttt acgttggtaa agggcgaaat cgggaagagg aaaacgacgc
     8341 tgaagcagaa agcgaaaaaa ctgaagaata aattcaaccg cccgtcaggg cggttgtcat
     8401 atggagtaca ggatgtttga tatcggcgtt aatttgacca gttcgcaatt tgcgaaagac
     8461 cgtgatgatg ttgtagcgcg cgcttttgac gcgggagtta atgggctact catcaccggc
     8521 actaacctgc gtgaaagcca gcaggcgcaa aagctggcgc gtcagtattc gtcctgttgg
     8581 tcaacggcgg gcgtacatcc tcacgacagc agccagtggc aagctgcgac tgaagaagcg
     8641 attattgagc tggccgcgca gccagaagtg gtggcgattg gtgaatgtgg tctcgacttt
     8701 aaccgcaact tttcgacgcc ggaagagcag gaacgcgctt ttgttgccca gctacgcatt
     8761 gccgcagatt taaacatgcc ggtatttatg cactgtcgcg atgcccacga gcggtttatg
     8821 acattgctgg agccgtggct ggataaactg cctggtgcgg ttcttcattg ctttaccggc
     8881 acacgcgaag agatgcaggc gtgcgtggcg catggaattt atatcggcat taccggttgg
     8941 gtttacgatg aacgacgcgg actggagctg cgggaacttt tgccgttgat tccggcggaa
     9001 aaattactga tcgaaactga tgcgccgtat ctgctccctc gcgatctcac gccaaagcca
     9061 tcatcccggc gcaacgagcc agcccatctg ccccatattt tgcaacgtat tgcgcactgg
     9121 cgtggagaag atgccgcatg gctggctgcc accacggatg ctaatgtcaa aacacggttt
     9181 gggattgcgt tttagagttt gcggaactcg gtgttcttca cactgtgctt aatctcttta
     9241 ttaataagat taagcaatag catggagcga gcctcaccat cgggttcggt gaaaatggcc
     9301 tgaaagcctt cgaacgcgcc ttcggtaata atcaccttat ctcccggata aggggttgcc
     9361 ggatcgacaa tgtctttcgg tttatatacc gatagctgat gaatcaccgc cgatgggact
     9421 atcgctagcg acgcgccaaa gcgcacgaag tggctgacac cgcgggtcgc gttgatagtc
     9481 gtggtatgaa tcacttctgg atcaaattcg acaaacaggt agttgggaaa caatggctca
     9541 ctgactgcag tacgttttcc acgcacgatt ttttccaggg tgatcatcgg tgccaggcaa
     9601 ttcacagcct gtctctcaag gtgttcctgg gcacgttgaa gttgcccgcg cttgcagtac
     9661 agtaaatacc aagattgcat aatgactctt atccgtttaa tcggggcgca aggatagcaa
     9721 aagctttacg ctaagttaat tatattcccc ggtttgcgtt ataccgtcag agttcacgct
     9781 aatttaacaa atttacagca tcgcaaagat gaacgccgta taatgggcgt agattaagag
     9841 gctacaatgg acgccatgaa atataacgat ttacgcgact tcttgacgct gcttgaacag
     9901 cagggtgagc taaaacgtat cacgctcccg gtggatccgc atctggaaat cactgaaatt
     9961 gctgaccgca ctttgcgtgc cggtgggcct gcgctgttgt tcgaaaaccc taaaggctac
    10021 tcaatgccgg tgctgtgcaa cctgttcggt acgccaaagc gcgtggcgat gggcatgggg
    10081 caggaagatg tttcggcgct gcgtgaagtt ggtaaattat tggcgtttct gaaagagccc
    10141 gagccgccaa aaggtttccg cgacctgttt gataaactgc cgcagtttaa gcaagtattg
    10201 aacatgccga caaagcggct gcgtggtgcg ccctgccaac aaaaaatcgt ctctggcgat
    10261 gacgtcgatc tcaatcgcat tcccattatg acctgctggc cggaagatgc cgcgccgctg
    10321 attacctggg ggctgaccgt aacgcgcggc ccgcataaag agcggcagaa tctgggcatt
    10381 tatcgccagc agctgattgg taaaaacaaa ctgattatgc gctggctgtc gcatcgcggc
    10441 ggcgcgctgg attatcagga gtggtgtgcg gcgcatccgg gcgaacgttt cccggtttct
    10501 gtggcgctgg gtgccgatcc cgccacgatt ctcggtgcag tcacccccgt tccggataca
    10561 ctttcagagt atgcgtttgc cggattgctg cgcggtacca agaccgaagt ggtgaagtgt
    10621 atctccaatg accttgaagt gcccgccagt gcggagattg tgctggaagg gtatatcgaa
    10681 caaggcgaaa ctgcgccgga agggccgtat ggcgaccaca ccggttacta taacgaagtc
    10741 gatagtttcc cggtatttac cgtgacgcat attacccagc gtgaagatgc gatttaccac
    10801 tccacctata ctggtcgtcc gccggatgaa cccgcggtgc tgggtgtcgc actgaacgaa
    10861 gtgtttgtgc cgattctgca aaaacagttc ccggaaattg tcgattttta cctgccaccg
    10921 gaaggctgtt cttatcgtct ggcggtagtg acaatcaaaa aacagtacgc cggacacgcg
    10981 aagcgcgtca tgatgggcgt ctggtcgttc ttacgccagt ttatgtacac taaatttgtg
    11041 atcgtttgcg atgatgacgt caacgcacgc gactggaacg atgtgatttg ggcgattacc
    11101 acccgtatgg acccggcgcg ggatactgtt ctggtagaaa atacgcctat tgattatctg
    11161 gattttgcct cgcctgtctc cgggctgggt tcaaaaatgg ggctggatgc cacgaataaa
    11221 tggccggggg aaacccagcg tgaatgggga cgtcccatca aaaaagatcc tgatgtcgtc
    11281 gcgcatattg acgccatctg ggatgaactg gctattttta acaacggtaa aagcgcctga
    11341 tgcgcgttag ttttgcccta tttatttgat ccgacagaga aagcgcatga caaccttaag
    11401 ctgtaaagtg acctcggtag aagctatcac ggatactgta taccgtgtcc gaatcgtacc
    11461 agacgcggcc ttttcttttc gtgctggtca gtatttgatg gtagtgatgg atgagcgcga
    11521 caaacgtccg ttttcaatgg cttcgacgcc ggatgaaaaa gggtttatcg agctgcatat
    11581 tggcgcttct gaaatcaacc tttacgcgaa agcagtcatg gaccgcatcc tcaaagatca
    11641 tcaaatcgtg gtcgatattc cccacggaga agcgtggctg cgcgatgatg aagagcgtcc
    11701 gatgattttg attgcgggcg gcaccgggtt ctcctatgcg cgctcgattt tattgacggc
    11761 gttggcgcgt aatcccaatc gcgatatcac catttactgg ggtgggcgtg aagagcaaca
    11821 tctgtatgat ctctgcgagc ttgaggcgct ttcgctgaag catcctggtc tgcaagtggt
    11881 gccggtggtt gaacaaccgg aagcgggctg gcgtgggcgt actggcaccg tgttaacggc
    11941 ggtcttgcag gatcatggta cgctggcaga gcatgatatc tatattgccg gacgttttga
    12001 gatggcgaaa attgctcgtg acctgttttg cagtgagcgt aatgcgcggg aagatcgtct
    12061 gtttggcgat gcgtttgcat ttatctgaga tataaaaacc cgcccctgac aggcgggaag
    12121 aacggcaact aaaactttat tcagtggcat ttagatctat gtcgcatccg gcaatcggtg
    12181 cacgatgcct gatgcgacgc tggcgcgtct tatcaggcct acattggtgc aggatcggta
    12241 gaccggataa ggcgttcacg ccgcatccgg caagtggtta aacccgctca aacaccgtcg
    12301 caataccctg acccagaccg atacacatcg tcgccagacc aaactgaacg tctttgtgtt
    12361 ccatcagatt cagcaacgtg gtgctgatac gcgcaccgga acaacccagc ggatgaccca
    12421 gcgcgatcgc accaccattg aggttgatct tctcgtcaat ctgctccatt agcccgagat
    12481 ctttaatgca cggcaggatc tgcgcggcaa aggcttcgtt catctcaaac acgccgatat
    12541 cgctggcaga aagccccgct tttttcagcg ccagcttcga ggcaggaacc gggccgtaac
    12601 ccataatcga cggatcacaa ccaacgaccg ccatcgaacg cacacgggcg cgcggcttaa
    12661 gacctaattc acgggcgcgg ctttcactca tcaccagcat ggccgcggca ccatcagaaa
    12721 gtgcagaaga tgtacccgcc gttaccgtac cgcttactgg atcaaacgcc ggacgcagcg
    12781 tggcgagggc ttccacggtg gtttccgggc gaatcacttc gtcgtaatta aactgcttca
    12841 ggacgccgtc ggcatcgtga ccaccggtcg ggatgatttc atttttaaat gcgcccgact
    12901 gcgtggcggc ccaggcgcgg gcgtgtgacc gcgcggcaaa ggcatcctgc atttcacggc
    12961 tgataccgtg catacgcgcc agcatttctg ccgttaagcc catcatgccc gccgctttgg
    13021 cgacattgcg gctcaggccg gggtgaaaat cgacgccgtg actcatcggc acatggccca
    13081 tatgctccac gccgccaacc agacatgcct gcgcatcgcc agtcatgatc attcgtgctg
    13141 cgtcatgcag tgcctgcatg gatgaaccac acaagcgatt gacggtaacc gccgggacag
    13201 agtgcggcac ttctgccagc agcgccgcgt tacgggcgat attaaaaccc tgctccagcg
    13261 tctgctgcac acaaccccag taaatatcgt cgagggccgc cgcttccagc gccgggttac
    13321 gcgccagcag gctacgcatt aaatgagcgg agagatcttc tgcacgcacg ttacgaaaag
    13381 caccgccctt cgaacggccc atcggggtgc gaattgcatc gacaatgaca acctgttcca
    13441 ttgtgactcc ttaagccgtt ttcaggtcgc caaccggacg ggctggctca accggaggat
    13501 agtacggttc gttatgacgc gctttattac gcagaccttc cggcacttca tacagcgggc
    13561 cgaggtgctg atattgctgt gccatatcga ggtattttgc gctaccgagg gtgtccagcc
    13621 agcggaacgc gccgccgtgg aacggaggga agcccaggcc gtagaccagc gccatatccg
    13681 cttccgccgg agtggcgata atgccctctt ccagacagcg caccacttcg ttgaccatcg
    13741 ggatcatcat gcgggcgata atctcttctt cgctgaaatc gcgcttcggc tggctcactt
    13801 ctgccagcag gtcttcaacg gcggcgtctt cttctttctt cggcttacct ttgctgtctt
    13861 ctttataacg ccagaaaccg aggccgttct tctgaccaaa gcggttggca tcaaacagcg
    13921 cgtcgatggc atcgcggtaa tctttttgca tccgctgcgg gaagcctgct gccatgacag
    13981 cctgagcgtg atgcgcggta tcaatgccca caacgtccag cagatatgcc gggcccatcg
    14041 gccagccaaa ctgtttttcc atcactttgt cgatcttgcg gaaatccgcg ccgtcgcgca
    14101 gcagctggct gaaaccggcg aaatacggga acagcacgcg gttaacaaag aagccggggc
    14161 agtcgttaac cacaatcggc gtcttgccca tcttgctcgc ccaggcgaca actttcgcga
    14221 tggtttcgtc ggagcttttc tcgccgcgaa taatttctac caacggcatt cggtggaccg
    14281 ggttaaagaa gtgcatcccg cagaagtttt ccgggcgttc cagcgcgttg gccagttcgc
    14341 tgataggaat ggttgaagtg ttagacgcca gcacggtatc ctggcgtact ttttgttcgg
    14401 tttctgccag tacggctttt ttcactttcg ggttttcaac aaccgcttct accacaatat
    14461 ccacgcggtc aaatccggcg tagtcgagcg ttgggtggat cgtggagatc acgccagcca
    14521 gtttcagacc atcgatcttg ccgcgctcaa gctgcttgtt cagcagtttc gcggcttcgg
    14581 tcatgctgag ggttaacgac ttgtcgttga tatctttcat gacaaccggc acgcctttcc
    14641 acgcggactg gtaagcgata ccgccaccca tgatgcctgc gcccagcaca gcagcctgtt
    14701 tcggggtttc aacgtctttg gtgagtttct tcgctttgcc ttttacatat tgatcgttaa
    14761 ggaaaatgcc gaccagtgcg cgagcttcgt tggtatgcgc cagcgggaca aaacttttgt
    14821 tttccagatt taaggcttct tcacgaccaa aacgggccgc agcttcaatg gtttttactg
    14881 cggtgatggg ggccggataa tgtttccccg ccgtttgcgc gaccattcct ttagcgatgg
    14941 tgaagctcat ggtggcttca atcttgctca gttttagcgg ttccagcttc ggctggcgtt
    15001 tcgctttcca gtcgaggtcg ccgttaatgg cctggcgtaa aaccgccttt gcgccttcaa
    15061 ccagtttttc tgctttgact accccatcca ccagaccgat tttcagcgcc tgatccgcgc
    15121 cgacatcttt accggcggca atgatttcca gcgcactgtc agcgcccagc atacgtggca
    15181 tacgtacaga accgccaaag cccggcatga tgcccagttt ggtttccggc agaccgatgc
    15241 gcagatccgg cgtcgccaga cgataatcgg tcgccagcac gcattcgcag ccaccaccca
    15301 gcgcatagcc gttgacggca gcaatggtcg gcaccggcag atcttccagg cgattaaaca
    15361 cgctattggc aaagtgcagc cactgactta actgttcttc aggaacgagg aacagggaca
    15421 aaaattcggt gatatcagca ccgacgataa aggctgcttt gttcgaacgc agcagcagcc
    15481 cttttagatc tgattgctgt tccagcacgc cgatggcctc gccgaggctg gcgacggtcg
    15541 cagtgtcgag tttattaact gaacctgggg catcaaatac cagttcggca atgccatctt
    15601 ccagccagtc aaggtacagg gtgtcgcctt tgtaaagcat gtcagtctcc tgaatccgca
    15661 aggtgatctg gtcgtaccag atgcatcgaa gtgtgtattt tgtgttaaaa atatgcaaat
    15721 aaaagattaa agaaatgccg atctgatcac gctcggcaga aatcacgctc tggatgaacg
    15781 atgtgctaag atgcggagac ttaaggtcaa aaaaacagaa gggtaaaaaa tggaatcact
    15841 ggcctcgctc tataaaaatc atatagctac cttacaagaa cggactcgcg atgcgctggc
    15901 gcgcttcaag ctggatgcat tacttattca ctccggcgag ctgttcaacg tttttctcga
    15961 cgatcatccc tatccgttta aagtgaaccc gcaattcaaa gcgtgggtgc cggtaactca
    16021 ggtgccaaac tgctggttgc tggtggatgg cgtgaacaag ccgaaactgt ggttctatct
    16081 gccggttgat tactggcaca acgtcgaacc gctgccgacc tccttctgga ctgaagatgt
    16141 ggaagtgatc gcactgccga aagccgatgg cattggtagc ctgctgcccg ctgcgcgcgg
    16201 caatatcggt tatatcggtc cggtgccgga acgtgcgctg caactgggta ttgaggccag
    16261 caacatcaac ccgaaagggg tgatcgacta cctgcattac taccgctcct tcaaaaccga
    16321 gtacgaactg gcctgtatgc gtgaagcgca gaaaatggcg gtcaacggtc atcgtgcggc
    16381 agaagaagcg ttccgttctg gcatgagcga gttcgatatc aacatcgcct atctgaccgc
    16441 gaccggtcat cgtgataccg acgtacctta cagcaacatt gtggcgctta acgaacacgc
    16501 ttcggtgctg cattacacca aactggatca tcaggcaccg gaagagatgc gcagcttcct
    16561 gctggatgcc ggggccgaat ataacggcta tgcggctgac ctgactcgta cctggtcggc
    16621 aaaaagcgac aacgactacg cacagctggt gaaagacgta aatgatgaac aactggcgct
    16681 gatcgccacc atgaaagcag gcgttagcta tgtggattac cacatccagt tccatcagcg
    16741 catcgccaaa ctgctgcgta aacatcaaat catcaccgat atgagtgaag aagcgatggt
    16801 cgaaaacgat ctcaccggac cgtttatgcc gcatggtatc ggtcatccgc tgggcctgca
    16861 ggtgcatgac gtagccggtt ttatgcagga tgatagcggt acacacctcg cggcaccggc
    16921 aaaatatccg tacctgcgct gcacccgtat tctccagccg ggtatggtgt taaccatcga
    16981 accgggtatc tacttcattg aatcgctact ggcaccgtgg cgtgaagggc agttcagcaa
    17041 gcacttcaac tggcagaaaa ttgaagcact gaaaccgttc ggcggcattc gtatcgaaga
    17101 caacgtggtg atccacgaaa acaacgtgga aaacatgacc cgggatctga aactggcgtg
    17161 atggaaagct ggttaattcc tgcggcaccg gtcacggtcg ttgaagagat caaaaaaagc
    17221 cgcttcataa cgatgttggc gcataccgat ggcgttgagg cggcgaaagc gtttgttgaa
    17281 tcggtgcggg cagaacaccc cgatgcccgc caccattgcg tggcgtgggt ggcgggtgcg
    17341 ccggatgatt ctcaacagct gggtttctct gacgacgggg agccggcagg aacggcaggt
    17401 aaaccgatgc tcgcccagct aatgggcagc ggcgtcgggg aaattaccgc tgtggtagtg
    17461 cgctactacg gcggcatatt gcttggcacc ggtgggttag tgaaagcgta tggcggcggc
    17521 gtgaatcagg cgctgcgcca gctaacgacc caacgcaaga cgccattaac cgaatatact
    17581 ttgcaatgtg aatatcatca gttaaccggc attgaagcgt tgctggggca gtgtgacggc
    17641 aaaattatca acagtgatta tcaggcattc gttctgctgc gggtggcgct tccggcggcg
    17701 aaagtggctg aattttccgc aaagctggcg gattttagcc gtggttcatt gcaattgtta
    17761 gcgattgaag aataatcccc acttcgtttt gcagaactaa ggaagcggca gagatgcatt
    17821 ttcgcgccat tacccgaatc gttggactac tggtcatctt attttcaggg accatgatta
    17881 tccctgggct ggtagcactc atctaccggg atggagcggg ccgcgctttt acccagacct
    17941 tttttgtcgc cctcgccatt ggttctatgc tgtggtggcc gaaccgtaaa gagaagggcg
    18001 aactgaaatc ccgtgagggg tttctgatag tggtgctgtt ctggaccgtg ctgggtagtg
    18061 tcggtgcgct cccttttatc ttctcggaaa gcccgaacct cacgattacc gatgcgtttt
    18121 ttgaatcttt ctctggcctg accaccaccg gggccactac gctggtgggg ctggattcgc
    18181 tccctcatgc catcctcttt tatcgccaga tgctgcaatg gtttggcggg atggggatca
    18241 tcgtgttagc ggttgcgata ctgcctatcc tcggcgtggg tgggatgcag ctctatcgcg
    18301 cagaaatgcc cggcccgctg aaagataaca aaatgcgccc gcgaattgcg gaaacggcga
    18361 aaaccctgtg gttgatttat gtcttgctga ccgtcgcctg tgcgctggcg ttgtggtttg
    18421 ccggaatgga tgcctttgat gccatcggcc atagctttgc gactatcgct attggcggct
    18481 tctcgacaca tgatgccagt atcggttatt tcgacagccc gactattaac actatcattg
    18541 ctatcttcct gctgatctcc ggctgtaact acggtctgca cttttcactg ttaagtgggc
    18601 gtagtctgaa ggtttattgg cgcgatccgg aatttcgcat gtttatcggc gtacagttta
    18661 cgctggtggt tatttgtaca ctcgtactgt ggtttcataa tgtctacagt tcggcgctga
    18721 tgacaattaa ccaggcgttt ttccaggtgg tatcgatggc gacaaccgcc gggtttacga
    18781 ctgacagcat tgcccgctgg ccgctctttt tgccggtact gcttttatgt tcagctttta
    18841 tcggcggttg cgccgggtca acgggcggtg gcctgaaagt gatccgcatc ctgctgctgt
    18901 ttaagcaggg gaaccgtgag ctgaaacgac tggtgcatcc gaacgccgtc tatagcatta
    18961 agctggggaa tcgcgcactg ccggaacgta tcctcgaagc cgtttgggga tttttctccg
    19021 cctatgcatt ggtgtttatt gtcagtatgc tggcgattat cgccacgggc gtggatgact
    19081 tttctgcctt tgcttcagtt gttgcgacat tgaataacct ggggccaggg cttggcgtgg
    19141 ttgctgataa ctttaccagt atgaacccgg tggctaaatg gatcctgatt gccaacatgc
    19201 tgtttggtcg tctcgaggtc tttacattgc tggtgctctt taccccgact ttctggcgtg
    19261 aatgatggag taatacgtga aaacattaat tcttttctca acaagggacg gacaaacgcg
    19321 cgagattgcc tcctacctgg cttcggaact gaaagaactg gggatccaga cggatgtcgc
    19381 caatgtgcac cgcattgaag aaccacaatg ggaaaattat gaccgtgtgg tcattggtgc
    19441 ttctattcgc tatggtcact accactctgc gttccaggaa tttgtcaaaa aacatgcgac
    19501 gcgactgaat tcgatgccga gcgcctttta ttccgtaaac ctggtggcgc gcaaaccgga
    19561 gaagcgtact ccacagacca acagctacgc gcggaagttt ctgatgaact cgcaatggcg
    19621 tcccgatcgc tgcgcggtca ttgccggggc gctgcgttac ccacgttatc gctggtacga
    19681 tcgttttatg atcaagctaa ttatgaagat gtcaggcggt gaaacggata cgcgcaaaga
    19741 agttgtctat accgattggg agcaggtggc gaatttcgcc cgagaaatcg cccagttaac
    19801 caacaaaccg acgctgaaat aagcataaag aataaaaaat gcgcggtcag aaaattattt
    19861 taaatttcct cttgtcaggc cggaataact ccctataatg cgccaccact gacacggaac
    19921 aacggcaaac acgccgccgg gtcagcgggg ttctcctgag aattccgaca gagaaagcaa
    19981 aaataaatgc ttgactctgt agcgggaaag cgtattatgc acaccccgcg ccgctgagaa
    20041 aaagtgaagc ggcactgctc tttaacaatt tatcagacaa tctgtgtggg cactcgaaga
    20101 tacggattct taacgtcgca agacgaaaaa tgaataccaa gtctcaagag tggacacgta
    20161 attcattacg aagtttaatt ctttgagcat caaactttta aattgaagag tttgatcatg
    20221 gctcagattg aacgctggcg gcaggcctaa cacatgcaag tcgaacggta acaggaagca
    20281 gcttgctgct tcgctgacga gtggcggacg ggtgagtaat gtctgggaaa ctgcctgatg
    20341 gagggggata actactggaa acggtagcta ataccgcata a
//
LOCUS       DAZPEP010000063        20308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZPEP010000063 DAZPEP010000000
VERSION     DAZPEP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.063"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            218..718
                     /gene="tssB"
                     /locus_tag="ACHUT9_002276"
     CDS             218..718
                     /gene="tssB"
                     /locus_tag="ACHUT9_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFR1348739.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            753..977
                     /locus_tag="ACHUT9_002277"
     CDS             753..977
                     /locus_tag="ACHUT9_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348740.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1028..2503
                     /gene="tssC"
                     /locus_tag="ACHUT9_002278"
                     /pseudo
     CDS             1028..2503
                     /gene="tssC"
                     /locus_tag="ACHUT9_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2510..2923
                     /gene="tssE"
                     /locus_tag="ACHUT9_002279"
     CDS             2510..2923
                     /gene="tssE"
                     /locus_tag="ACHUT9_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFR1348741.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2927..4777
                     /gene="tssF"
                     /locus_tag="ACHUT9_002280"
     CDS             2927..4777
                     /gene="tssF"
                     /locus_tag="ACHUT9_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFR1348742.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4741..5823
                     /gene="tssG"
                     /locus_tag="ACHUT9_002281"
     CDS             4741..5823
                     /gene="tssG"
                     /locus_tag="ACHUT9_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFR1348743.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5848..7128
                     /gene="tagH"
                     /locus_tag="ACHUT9_002282"
     CDS             5848..7128
                     /gene="tagH"
                     /locus_tag="ACHUT9_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFR1348744.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHUT9_002283"
     CDS             7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHUT9_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFR1348745.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7652..8983
                     /gene="tssK"
                     /locus_tag="ACHUT9_002284"
     CDS             7652..8983
                     /gene="tssK"
                     /locus_tag="ACHUT9_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFR1348746.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKIFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPCIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8988..9749
                     /gene="icmH"
                     /locus_tag="ACHUT9_002285"
     CDS             8988..9749
                     /gene="icmH"
                     /locus_tag="ACHUT9_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFR1348747.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9758..12541
                     /gene="tssH"
                     /locus_tag="ACHUT9_002286"
                     /gene_synonym="clpV"
     CDS             9758..12541
                     /gene="tssH"
                     /locus_tag="ACHUT9_002286"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFR1348748.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAEPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGVEVVIEPEVTDEIM
                     SRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            12538..13281
                     /gene="vasI"
                     /locus_tag="ACHUT9_002287"
     CDS             12538..13281
                     /gene="vasI"
                     /locus_tag="ACHUT9_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFR1348749.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13286..14698
                     /gene="tssA"
                     /locus_tag="ACHUT9_002288"
     CDS             13286..14698
                     /gene="tssA"
                     /locus_tag="ACHUT9_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFR1348750.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGQLAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14807..18241
                     /gene="tssM"
                     /locus_tag="ACHUT9_002289"
                     /pseudo
     CDS             14807..18241
                     /gene="tssM"
                     /locus_tag="ACHUT9_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            18252..19701
                     /locus_tag="ACHUT9_002290"
                     /pseudo
     CDS             18252..19701
                     /locus_tag="ACHUT9_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19628..20110
                     /locus_tag="ACHUT9_002291"
     CDS             19628..20110
                     /locus_tag="ACHUT9_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR1348751.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
BASE COUNT         4800 a         5553 c         5612 g         4343 t
ORIGIN      
        1 gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg atcgataaaa
       61 agtccttttc ttatttattt tgaagcgtaa aatgcggggt gatgaaggag tggtgatttt
      121 attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa tatgccgtgt
      181 tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat ttgaaggcag
      241 cgtcgcgccc cgcgagcgta ttaatatatc gtatgtccca aaaacagatg gccagacggc
      301 tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata cgcaggaaac
      361 atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg gtgcagtgat
      421 ggctgaagca gccattggcc tcaattttac tgtccccgcc acgctgaagg gaagcacaac
      481 agacgatgaa ctgaatgtgg cgctgaatat caaatcactg gatgattttt caccagatag
      541 cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag cactgactgc
      601 actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg cattgctgga
      661 aaatgaagag tcccgcgagc agttgctcaa agaaatcggt ctggtgagca ataaataacc
      721 accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat atctccggga
      781 cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt ttcagcccgt
      841 ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat cgccgtatca
      901 gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg tcgcactggc
      961 agcgcgtact tcactgatgg cgaaaagccg gtttcagaaa cacagctaaa acagaaggat
     1021 tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc agcctgagca
     1081 ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg gttcggaagg
     1141 ttatgacgtt gccctccagg gtgtgacagc ctttattgcc agcatcctgc aatccactgc
     1201 atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata ttgatgaacg
     1261 catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt
     1321 ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa
     1381 cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc cagaaattat
     1441 tcagtccgga ttttataagc acgtttattc gtccggtttc ggacagtttg gcggtaaacc
     1501 catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca tgaaactgct
     1561 gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc
     1621 ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc tttatgccat
     1681 ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag attcgcgcta
     1741 tttagggctt acggcacccc gcttcctgct tcgccagccc tattcaccaa cagataaccc
     1801 tgttaagaac tttaactatt acgaagatgt cagccagaac cacgaagatt atctgtgggg
     1861 taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat accgctgatg
     1921 tccaaacatt atcggtccgc aaagcggcgg cgcagtgaaa gatctgccga tgcatctgtt
     1981 cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca ccgatcgccg
     2041 cgaatttgaa ctggctgaag agggttttat cacccttacc atgcgtaaag actctgataa
     2101 tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg gaaaagatgc
     2161 agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca atcggttagc
     2221 gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa ggagcgactt
     2281 agaaagagag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc
     2341 agacgtgcgc agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg
     2401 cgaacccggc tggtaccagg ttgcgctaag cgtgagacct cattttaaat ttatgggggc
     2461 aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca tgataccgga
     2521 tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg
     2581 gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac gaaacattct
     2641 taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg aaccggaagg
     2701 ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag aacgctatga
     2761 accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca gcccgctgga
     2821 tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg tgcttgaatt
     2881 tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg aatttgaaga
     2941 acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc tggcaacgga
     3001 aaaaccccat ctggcccgct tcctggccga aaaagatgca gatccggata ttgaacgcct
     3061 gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg atgaattccc
     3121 agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc cggttccggc
     3181 aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg tgttaatccc
     3241 ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc
     3301 tgatgctaaa aaggaggagc cgcctccctg taccttcacc ctctgccggg atatctggct
     3361 gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa
     3421 catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga acaaacttcg
     3481 cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat ggttctgcga
     3541 atacttgcag ggtgccgacc tgactgtggg tgaacagcat attcgcctgc ctgagtttat
     3601 gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga aaaacgtcca
     3661 cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc tcttttttga
     3721 tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta ccctgcaact
     3781 gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta
     3841 ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc tggacaaccg
     3901 gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg atgtattttc
     3961 cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc tggggcgtcc
     4021 tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc atcagataga
     4081 atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat ccctgttcca
     4141 tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc cgtcagacga
     4201 atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc
     4261 gtcacaaatt cgttccggcg atatcaccgg cacaaccgga aaaaatgcgg ctgttgcttc
     4321 atttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg gcagcctgca
     4381 ctggccccta ctctccgcca tgaacctgaa ttatctgtca ttactggata cggacgcgct
     4441 gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga cggcacggct
     4501 gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg atcgcctgtt
     4561 tacgggtatt cccgtccggg ggctggcttc cacgctgtat ctgcacccgg agccgtttgt
     4621 ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt cgctgtacgc
     4681 cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg agacatggaa
     4741 atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc cccggcagat
     4801 aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta caattttttt
     4861 gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc agaattatcc
     4921 ctgcgcgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc
     4981 ccgtgcagtg atatcagtgc gctgaaacgg gatacgtcag gggcattcag gatgaccacc
     5041 acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct cgctcacctg
     5101 gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat gttcagccac
     5161 cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat cagttttcgt
     5221 aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc
     5281 cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc cgggatcctg
     5341 gcaaatccgg gacgatcccc ggaaattatc tgtggcctgg tgtcccactg ctttgatttg
     5401 tcagaagtga cactgcaaaa ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa
     5461 aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg
     5521 ggcaactttg ttctgggtac acgagtgccc gatctcagcg gaaaattcca gctcagtatt
     5581 accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa cttcctgcca
     5641 ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt acatctgggg
     5701 ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc
     5761 tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag ggtacggtcg
     5821 taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac gctttcattg
     5881 caggtcatta acggcagtga gctggaaagc gggcgggcgg cgcgctgtct gttcacacag
     5941 cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg tcagcagagc
     6001 attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct gcgcccgcag
     6061 acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct aatacagttg
     6121 cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct gaaccgggga
     6181 gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac caatcgcgat
     6241 atgctcacgg ataccctgct atcaacggag ggtgcgccac actatccggg aatgactcac
     6301 cggcaccagc ttgcagacac cgtggtaaac ggtttttctg ccgatccact ccaggctctt
     6361 cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc tgtccggcca
     6421 tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg
     6481 ccgcccattt atgccagccc cggcgatcgt aatgacgacg tctctgcggc agaaatggcg
     6541 caacgccacg ttgcggtcac ccccttactg cgcggtcttg gcggctcgct taccatgagc
     6601 aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca ggccgcaata
     6661 aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca tttgcgcccg
     6721 ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtaatgttt
     6781 gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga aagcctgcgc
     6841 aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcgct tcgtgtcctg
     6901 ctggatgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat
     6961 gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa
     7021 gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccag
     7081 gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt
     7141 ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca
     7201 gtgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa ccttcggaaa
     7261 taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggaaggt gaatcagccg
     7321 ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca gccgactatg
     7381 accagatagc cagcacgccg ctaccggatg tgctgggtaa aaactatatc gaccaccagg
     7441 atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa
     7501 cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa
     7561 tcgagacggt ggaaggaatc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca
     7621 gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca aggtgatgtg
     7681 gcaggaaggg atgctgatgc gtccacacca tttccagcag cagcagcgtt acaacgacta
     7741 cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac
     7801 cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt caggcacact
     7861 gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc
     7921 gcaaaatttt ccggacgaaa gaagccgcaa tatctacctc gctctgccgg tcgccagtga
     7981 tgtgagaaat gaaatcagcg acggacggcg aatcgggcgt taccggctga attatgccga
     8041 tgtccgggat ttgcattcag aagaaggtga cgcgcgaaca ttgacgctgg gtcaactgac
     8101 gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc cgctttgccg
     8161 tatcagcgat cgccatgccg atggctccct gacgttggat gacgatttta tcccctcctg
     8221 ccagaatatt caggtcagta agaaactgcg tgtttatctc aaagaggtac agggggccat
     8281 tggcggacgg gcaagcgatc tggcaaaccg tattggctct ccggcgcaga gcggcatcgc
     8341 ggatgtggcg gaatttatga tgttgcagtt acttaaccgt aaccagaccc ggtttaccca
     8401 tcacgcccgt cgatcccagc tccacccgga agatttctac cttgatcttg ccgggttgct
     8461 gggtgaactg atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga
     8521 tcatcatgac ctgaccaaaa tatttaaaac gctgttaccg gaagtcaaac gggcgctgca
     8581 taccgtactg tcgccaagag ccgtcaatct gccgctgcat ctgcgtgacg gtatctggca
     8641 ggccgatatc catgacacag aactgctgca atctgccacc tttgtgctgg ccgtggcggc
     8701 aaatatgccg gtcgatcaga tccagcgcca gtttatccag cagtcgaaaa tttcctcgcc
     8761 ggaaaaaatc cgcaatatgg tcagtgtgca gatcccctgt attccattgc gtgcccttat
     8821 ggtggctccc cgccagcttc cttaccattc cgggttcagc tatttcgaac tcgacaagag
     8881 tggccaggcc tggacagaaa tggctgccgc cggggccgtt gcactgcatg tttccggcag
     8941 tttcccggat ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt
     9001 cactgtctct gccaccattt accggacacg acgaaaaaag ccagcgcaac taccatctcg
     9061 cgcttcgtgg caacagcctg aatccgatga tcgacgcggc cacgccgcta ctcggtatgg
     9121 taatgcgcct ttcaaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg
     9181 tgacagatgt tcaggccgtg gagcaactgc ttcaggagca gggctacgaa ccgggcgtga
     9241 ttatctcgtt tcgctacatc ctctgcacgt ttattgatga agcggcgctc ggtaacggct
     9301 ggtcaaacaa gaacgagtgg atcaagcagt cattgctggt ccatttccac aacgaagcct
     9361 ggggtggcga gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc
     9421 aggatctgct ggaatttctc tggatctgtt tttctctggg atttcgcggg cgctacaaag
     9481 tggcagcaca ggatcagggg gaatttgagc agatctaccg ccgtctgtac cacgttctgc
     9541 ataaactgcg cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccagggcg
     9601 ggcgttatca gctcatcagt cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg
     9661 tgctggcgtt gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc
     9721 tgcaccagct aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac
     9781 gctggtaaaa cggctgaacc tgttctcccg ccaggcgctg gagatggccg cctctgaatg
     9841 tatgagtcag caggcagcgg aaattaccgt cagccatgtg ctcattcaga tgctcgccat
     9901 gccacgcagt gacctgcggg ttattacccg ccagggcgat attggcatgg aagagttgcg
     9961 ccaggcgctg acggtagaga actacacaac cgcccgttct gcggacagct acccggcgtt
    10021 ttccccgatg ctggttgagt ggcttaaaga gggctggctg ctggcgtcgg ctgagatgca
    10081 gcacagcgaa ctgcgcggcg gcgtgttgct gctggccctg ctgcattcgc cgctgcgtta
    10141 tataccgcct gctgccgccc ggctgttgac cggcattaac cgtgaccgtc tgcaacagga
    10201 ctttgtgcag tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg
    10261 cgcaggcaca atgacggacg cctctgacac cctgcttgcc cgctatgcca aaaacatgac
    10321 cgcagacgcc cgtaacggca ggcttgaccc ggtactgtgc cgcgaccacg aaatcgacct
    10381 gatgatcgac attctctgcc gccgccgtaa aaacaacccg gtggtggtgg gcgaagcggg
    10441 cgtgggcaaa agcgcactga ttgaagggct ggcgctgcgc atcgtggcag gccaggtgcc
    10501 ggacaagctg aaaaacaccg atatcatgac ccttgacttg ggcgcattgc aggccggggc
    10561 gtcggtgaaa ggtgaattcg aaaaacgttt caaagggctg atggcggagg tcatttcctc
    10621 cccggtgccg gtcattctgt ttatcgacga agcacatacc ctgattggcg cgggcaacca
    10681 gcagggcggg ctggatatct ccaacctgct caaaccggcg ctggcgcgcg gcgagctgaa
    10741 aaccatcgcc gccaccacct ggagcgagta caaaaaatac ttcgaaaaag atgccgccct
    10801 gtcgcgccgc ttccagttgg tgaaggtcag cgaacccaac gctgccgaag ccaccattat
    10861 tctgcgcggt ctgtcggcgg tctatgaaca gtctcacggc gtgctgattg atgatgacgc
    10921 cttgcaggcc gctgcgacat taagcgagcg ttatctctcc gggcgtcagt taccggacaa
    10981 agcgattgat gtgctggata ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc
    11041 gaagcaaatc tcggcgctga ccactctgag ccaccagcag gaggcggaaa ttcgccagct
    11101 tgagcgcgag cttcgcatcg gactgcgtac cgacacatca cggatgaccg aggtgctggt
    11161 gcagtatgat gaaacgctga cggcgctgga tgaactggaa gcggcctggc accagcagca
    11221 gacgctggtc cgggagatta tcgcgctgcg ccagcagtta ctgggcgtgg cagaggacga
    11281 tgcggcgccg ttgccggacg cagataccgt ggaggatacg cagccagagt cagagtcaga
    11341 gtcagagtca gagtcagagc aggataatac cggtgccgaa ccggctgatg aggccgacag
    11401 agaacaaccg gaagagaccg ctgaaacagt ttccccggta cagcggctgg cccagctcac
    11461 tgccgaactg gacgccctgc ataacgaccg gttgctggtc tccccgcacg tcgataaaaa
    11521 acagattgcg gcggtgattg ccgaatggac cggcgtgccg ctcaaccgcc tgtcgcagaa
    11581 tgaaatgtcg gtcatcaccg acctgacgaa atggctgggt gacaccatca aaggccagga
    11641 cctggcgatt gccagcctgc ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc
    11701 gggacgcccg ctcggtgcgt tcctgctggc tggccccagc ggtgtgggta aaaccgaaac
    11761 cgtcctgcaa ctggcagagc tgctctacgg cggtcgccag tacctgacca ccatcaatat
    11821 gtccgaattc caggagaaac acaccgtctc gcggctgatt ggttcgcctc cgggctacgt
    11881 tggctacggt gaaggcggcg tactgaccga agcgattcgc cagaaaccct actcggtagt
    11941 actgctcgat gaagtggaaa aagcgcaccc ggatgtgctc aacctgttct accaggcgtt
    12001 cgataagggc gaaatggcag acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt
    12061 cttcctgacg tccaacctcg gctaccaggt aatagtcgag catgccgatg acccggaaac
    12121 catgcaggaa gtactgtatc cggtgctggc cgacttcttc aaacctgccc tgctggcgcg
    12181 tatggaagtg gtgccgtatc tgccgctgtc gaaagagacg ctcgccacca ttatcgccgg
    12241 aaaactggcc cgtctggata acgtgctgcg cagtcgcttt ggtgtagaag tggtcattga
    12301 accggaagtg acggacgaaa tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat
    12361 gctggaatcg gtcatcgatg gcgacatgct accgccgctc tcgctgctgc tgttgcagaa
    12421 aatggcggct aacacggcga ttgcccggat tcggttgtcg gcagtggacg gcgcatttac
    12481 ggcagacgtg gaagatgctc agaacgacga gtccgtcaca aaggatgaaa cggttttatg
    12541 agcaggttgc gcgttttccg gcagacagga tggtttatcg cgggcctgat gacgggctta
    12601 cccgccactg ccgcgcccgc agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc
    12661 accacgaccc cgccggatgc gacagccacg ttacaggcca tgcagtcctg tcggcgggag
    12721 tccgcggcgc tggagcggct tgactgctat gaccgcctgc tggcaccgct gtccccgtca
    12781 gggtttgacg gggcgctggt caaagccggt tttgtgggcg aggcctggac ccgcgccaca
    12841 gagcaggaaa aacgccgcga gggcaacacc acagaactgc tggtgacgca ggtgccgggg
    12901 gagcgcccga cggtggtgat aaccaccccg gctatcggtc atgttccgcc acgcccggtg
    12961 ctgatgttca gttgcgtgga caatatcacc cggatgcagg tggcgctgat gcaccctctg
    13021 gatgtccatg atatcgccgt cactctgaat gccgacaacc gggcattgcg cagccactgg
    13081 tttgtgcgcg agaacggcac cctgctggag tccagccggg ggttatccgg gattgatgaa
    13141 attaagcaac tgttcggcgc aaaaacgctg acggtggata ccggcgcaga caatgccgcc
    13201 ggaaagctga cctttaacat tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc
    13261 tgccactggg cgggagaata aaaatatggc aatggactta cgcgatccga atgtctggat
    13321 atcgcacctg ctggaaaacc tgccggaaga gaaactggca tcggcgctga aagatgacaa
    13381 cccgaactgg gagtatatcg acggcgaaat cgtcaagctg ggatcgctgg cccacagtca
    13441 actggatatc ccggaactcc agcgccgggg gctggtcatt ctggcttcgg agagtaagga
    13501 ttttcgtctg ctggcgcatc tgctaagaac cctgcaacac gccggtgatc ctcatctcgc
    13561 attgcgcctg ctggcgctgt atgtggagca ttactgggcg gtggctgcgc cgcagaatat
    13621 ggcgcataaa aaacgctttg ccagccaggt cattaaacgc tttgaaacag gtattgaagt
    13681 tttttcacag aacgccgcca cagcgcagcg cgatgcgctg tcagggcaac tggcaaaact
    13741 ggcgcaatgc tggcagtcac ataacgcccc ggaactggca caggctactg atgatctttt
    13801 tgccctgtac cagcgtgcgt ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc
    13861 ctccggtagt tcaccacaga ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc
    13921 tccggctccc caaatcgtca tcgacagtca cgacgacaaa gcctggcgcg acacgctgtt
    13981 aaaagtggcg gccattttat gtgaacgcca gccggattca ccgcagggct accgtctgcg
    14041 ccgccatgcg ctttggcaga acatcaccag tacgccccag gcggaaagcg atggccgtac
    14101 tccactggct gcggtctccg ccgatatggt ggcagattac catgctcagc tcggtagtgc
    14161 cgatatggcg ctgtggcagc aggtggagaa aagcgtactg ctggctcctt actggctgga
    14221 tggtcactgc ctttctgcac agacggcgct gcgtctgggt tacaaacagg tggcagacgc
    14281 catccgcgat gaggtcatcc gcttccttga gcgtctgccc cagcttaccg ggctgctgtt
    14341 taatgaccac acaccgttta tcagtgagca gacgaaacaa tggctggcag cttcgcccga
    14401 cgccaaagtt gcaccggttg cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt
    14461 tgctgagcag ggtctggagg cggcgctgcg atatctggac atgctacccg aaggcgatcc
    14521 ccgcgatcag tttcaccgcc agtaccttgc cgcacagttg acggaagagg cggggctggt
    14581 acagcttgcg cagcagcagt accggatgtt gttcaggatg ggattgcaga tgatggtggc
    14641 agactgggag ccgtccctgc ttgagcagct agaacaaaaa ttcacggcag aacaataaaa
    14701 gagaacagga gtttttgtgt tcagattacc aacaccccga ctactcagcg gactcaaatc
    14761 agccctgcga ccggcgatgc cccggtttaa agtctctgct ttctggctgc tgatactggc
    14821 gtggattttt ctgctggtgt ggatctggtg gaaaggcccg atgtggacgc tgtatgaaga
    14881 acagtggctc aaaccactgg cgaaccgctg gctggcaacg gcggcgtggg ggattattgc
    14941 cctggtgtgg cttaccgtcc gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa
    15001 gcaacagcgc gaggaagccg ttgatccgct cagcgtggaa ctgaacgccc agcagcgtta
    15061 tcttgaccgc tggctgctgc gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca
    15121 gttgccgtgg tatatggtca tcggcccggc gggcagtggc aaaacgacac tgctgcgcaa
    15181 ggggtttccg tccgacatta tttatgcccc ggagggcgca cgcggcgcag aacaacgcct
    15241 gtacctcacg ccccatgtcg gtaaacaggc ggtgatcttt gatatcgacg gcacactctg
    15301 cgctcccgct gatgcggata tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct
    15361 gaaagaaaag cgcgcgcgcc agccgctgaa cgggattatt ctgacactcg atttacccga
    15421 tctgctgacc gcagacaaac gccgccgcga gcatctgtta cagacgctgc gcagccgctt
    15481 acaggatata cgccagcatc ttcactgcca gttaccggtt tacgtggtac tgacccggct
    15541 tgatttatta cagggttttg ccgccctgtt ccagtcactc aacagacagg atcgcgatgc
    15601 gattctgggc gtcacgttca cccgccgtgc ccatgaaaat gatgactggc gaacagagtt
    15661 gaatgctttc tggcagacat gggtggatcg aatgaatctg gcgttgccgg atctgatggt
    15721 cgctcagact cacacccgcg cgtctttatt cagtttttcc cgccagatgc agggaagccg
    15781 tgaaccgctg gtgtcactgc ttgagggtct gcttgatggc gaaaatatga acgtgatgct
    15841 gcgtggtgtc tatctcacct cttcgcttca gcgtggacag atggatgata tattcaccca
    15901 gtctgccgcc cgccagtacc ggctgggcaa taacccactg gcgtcctggc ccctggtgga
    15961 caccgcgcct tatttcaccc gcagcctgtt cccgcaggca ttactcgcag agcctaatct
    16021 ggcaacagag agccgcgcct ggctgatacg ttcccgtcgc cgcctgacgg ttttctctgc
    16081 cacaggcggc gtggcagcac tgctgctcat caccggctgg catcactatt acaacggtaa
    16141 ctatcagtcc ggcatcaccg tgcttaagca ggccaaagcc tttatggacg tgccgcctcc
    16201 gcagggggaa gatgactttg gcaacctgca actgccgctc ctgaacccgg tacgcgatgc
    16261 cacactggcc tatggcgact ggggcgaccg cagccgtctg gccgatatgg gactgtacca
    16321 gggacgacgt atcgggcctt atgtggaaca gacctatctg caactgctgg agcaacgtta
    16381 cctgccctcg ctgtttaacg ggctggtcaa agcgatgaac gccgcgccgc cggagagtga
    16441 agaaaaactc gcggtgctgc gcgtgatgcg aatgctggag gacaaaagcg gacgtaacaa
    16501 tcaggtggtg aagcagtata tggcaaaacg ctggagcgaa aagttccacg gtcagcgcga
    16561 tatccaggca caactgatgt cccatcttga ctacgcgctg gctcatactg actggcacgc
    16621 agagcgtcag gcgggcgacg gtgacgccat cagccgctgg acgccatatg acaagcccgt
    16681 ggtatcagca cagaaagaac tgagcaaact gcctgtctac cagcgggttt accagagcct
    16741 gaaaacgcgg gcgctgggcg ttcttcctgc cgacctcaat ctgcgtgacc aggtagggcc
    16801 aacctttgac caggtgttta catctgccga tgacaacaaa ctggttgttc cacagtttat
    16861 cacccgctac ggcctgcaaa gctattttgt aaaacagcgc gatgaactgg ttgaactgac
    16921 agcgatggat tcctgggtac ttaacctcac ccgcaatgtg aaatacagtg acgccgaccg
    16981 tgcggaaatc cagcaccagt tgaccgagca gtatatcagc gactacaccg ccacctggcg
    17041 ggctgggatg gacaatctga atatccgcaa ttttgagtcc atcggacaac tgaccggggc
    17101 gctggagcag gttatcagcg gcgaccagcc tttgcagcgg gcgctgaccg tgctgcgtga
    17161 cagcacacag ccaggcgtct tttctgaaaa actctctgcc aaagaacggg aggaagccct
    17221 ggcagagccg gattaccagt tactcacccg cctcgggcat gaattcgccc cggaaaacag
    17281 taccctggca gtacagaaag acaaagaaag cacgatgcag gccgtgtatc agcaactcac
    17341 cgagttgcac cgctacctgc tggcaatcca gaacgcgcct gtaccaggga aatcggcgct
    17401 gaaagccgtg cagttacggc ttgatcagaa cagcagcgat ccgatattcg ccacccgcca
    17461 gatggcaaaa acgctgcctg ctccgctcaa ccgctgggtt ggcagactga ctgactaggc
    17521 ctggcatgtg gtgatggtgg aggctgttca ttatatggaa gtggactggc gcgacagcgt
    17581 ggtgaaaccg tttaacgagc aactggcaaa taactatccg tttaatccgc gttctgcaca
    17641 ggatgcctca ctggatgcct tcgaacgctt ctttaaaccg gatggcatac tggatacctt
    17701 ctaccagcag aacctgaagc tgtttatcga taatgacctg agtctggagg atggcgataa
    17761 caacgtcatt attcgcgaag atattattgc gcaactggaa actgcgcaga aaatccgtga
    17821 catcttcttc agcaaacaga acggtctggg aacatccttt gccgtggaaa cggtatcgct
    17881 ttcaggcaat aaacgccgca gtgtactgaa ccttgacagt cagttagtcg attacagcca
    17941 gggccgtaac tataccgccc atctggtctg gcctaacaac atgcgcgaag gcaacgaaag
    18001 taagctgacg ctcatcggca ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc
    18061 gtgggcgcag ttccgcctgt tctgggccgg acaactgacc ggagtacagg atggcaactt
    18121 taccgtgcgc tttagcgtgg acggtggcgc gatgacctac cgtgtgcata ccgacacgga
    18181 agataacccg ttcagcggtg ggttgttcag ccagtttggt ctgtcagaca cactgtactg
    18241 aggaggcagg gatgaatagt aacgtactga cacaaactat cgttaccggc agtgacccgc
    18301 gcgggctgcc ggaattcagc gccatccgcg aggaaataaa caaagccagc cacccgtcac
    18361 agcctgagct gaactggaaa ctggtggagt cgctggcgct ggcgattttt aaagccaacg
    18421 gtgtggattt acacaccgcc acctactata cgcttgcccg gacacggaca cagggactgg
    18481 cgggattctg cgaaggtgcg gaactgctgg cggcaatggt aagccacgac tgggataagt
    18541 tctggccgca gggcggcccg gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg
    18601 gcaatattct gcgtcagcaa atctcctttg cggaatccga cctaccactg atctaccgca
    18661 cagagcgggc attgcagctt atctgcgata agctccagca ggtggaactg aagcgcgttc
    18721 cgcgcgtgga gaatctgctc tattttatgc agcacacgcg taaacggctt gaaccccagc
    18781 cgaagagtaa cgctgagaac gccgcacaga ccacggtcag aacgctgatt tatgccccgg
    18841 aaacactggc atcttccaca ccagaagcgg tagtgcctcc cctgcccggc ctgcctgaga
    18901 tgaaagtgga agtgcgcagt ctgacagaga atcccccaca ggccagtgtg ataaagcaag
    18961 gcagtacggt aagagggttt atcgcaggga tcgcctgttc agtggctgtc gcctcagcat
    19021 tgtggtggtg gcaggtctat ccggtgcagc agcaactgtt acaggttaac gacaccgctc
    19081 agggcgcagc aacggtgtgg atggcctcac ctgaactcga aaactatgag cgcaggctgc
    19141 aacaacttct tgatacctcc ccggtacagc cgctggaaac cgggatgcag atgatgcgtg
    19201 ttgccgacag tcgctggccg gaaagcctgc aacagcaaca ggcctcgaca caatggaatg
    19261 aggcactcaa aacccgcgca cagagtagcc cgcagttgcg tggctggttg cagacccgcc
    19321 aggacttaca tgcttttgca gatctagtga tgcagcgcga gaaagaggga ctaacccttt
    19381 cctatatcaa aaatgtcatc tggcaggcgg agcggggact ggggcaggaa acccccgttg
    19441 agtctctgtt gacgcagtac catgatgccc gtgcgcagaa gcagaataca gatgcgctgg
    19501 aaaaacaaat taatgagcga ctcgaaggcg tgttaagccg ctggctgctg ctgaagaata
    19561 acgtcatgcc agaggcggca accggtacca cggccgaaaa ataattattt aattaaagga
    19621 gattatcatg gcaaatataa gttatttatc tttgagtgga gagacacaag ggctaatttc
    19681 agcaggttgc tcaacgcttg attctgtcgg aaacaaagcg cagccagaac ataaagatca
    19741 gatcatggtt tatgcgttaa tgcattccat ctcaagaagt caaaatgtca atcatcatga
    19801 attaattatc accaaaccgg ttgataaatc ctcaccattg ctggcaaaag cacttagtga
    19861 caatgaaaaa atggcaatat gtgaatttat actctaccga accagtaaag cgggtattta
    19921 ccagccttat tataaaataa atctgtcaaa agcccgaata tcaagtattg attttgtcac
    19981 accgcatgct gtcctggaaa aagaactgga accacaggag agaattgcct ttatttatga
    20041 agatatttcc tgggagcata cgttagccgg tacgaatgca atgagtaaat ggcaggatcg
    20101 tgtacagtaa cttactgtaa taacataata aattactatt gcggagagat aaaatggctg
    20161 gtttaaggaa taacaacaac acacagaatg cacagtgggc tgattatgtg ggtgacattc
    20221 ttcggggggc tcagccgata aaccagctag tacctcagca cccttatctt aacgacgtcc
    20281 cactcattga tgagttaagg catcagaa
//
LOCUS       DAZPEP010000064        20281 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZPEP010000064 DAZPEP010000000
VERSION     DAZPEP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.064"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            358..1029
                     /locus_tag="ACHUT9_002292"
     CDS             358..1029
                     /locus_tag="ACHUT9_002292"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFR1348752.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1039..2235
                     /locus_tag="ACHUT9_002293"
     CDS             1039..2235
                     /locus_tag="ACHUT9_002293"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1348753.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIKRGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2235..2795
                     /locus_tag="ACHUT9_002294"
     CDS             2235..2795
                     /locus_tag="ACHUT9_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1348754.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2797..3570
                     /locus_tag="ACHUT9_002295"
     CDS             2797..3570
                     /locus_tag="ACHUT9_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFR1348755.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3758..4033
                     /gene="frmR"
                     /locus_tag="ACHUT9_002296"
     CDS             3758..4033
                     /gene="frmR"
                     /locus_tag="ACHUT9_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFR1348756.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4068..5177
                     /gene="frmA"
                     /locus_tag="ACHUT9_002297"
     CDS             4068..5177
                     /gene="frmA"
                     /locus_tag="ACHUT9_002297"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFR1348757.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5270..6103
                     /gene="fghA"
                     /locus_tag="ACHUT9_002298"
     CDS             5270..6103
                     /gene="fghA"
                     /locus_tag="ACHUT9_002298"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFR1348758.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6329..6868)
                     /locus_tag="ACHUT9_002299"
     CDS             complement(6329..6868)
                     /locus_tag="ACHUT9_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFR1348759.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6970..8181)
                     /gene="mhpT"
                     /locus_tag="ACHUT9_002300"
     CDS             complement(6970..8181)
                     /gene="mhpT"
                     /locus_tag="ACHUT9_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFR1348760.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8468..9481)
                     /gene="mhpE"
                     /locus_tag="ACHUT9_002301"
     CDS             complement(8468..9481)
                     /gene="mhpE"
                     /locus_tag="ACHUT9_002301"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFR1348761.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYLSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9478..10428)
                     /gene="mhpF"
                     /locus_tag="ACHUT9_002302"
     CDS             complement(9478..10428)
                     /gene="mhpF"
                     /locus_tag="ACHUT9_002302"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFR1348762.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10425..11234)
                     /gene="mhpD"
                     /locus_tag="ACHUT9_002303"
     CDS             complement(10425..11234)
                     /gene="mhpD"
                     /locus_tag="ACHUT9_002303"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFR1348763.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11244..12110)
                     /gene="mhpC"
                     /locus_tag="ACHUT9_002304"
     CDS             complement(11244..12110)
                     /gene="mhpC"
                     /locus_tag="ACHUT9_002304"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFR1348764.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(12128..13072)
                     /gene="mhpB"
                     /locus_tag="ACHUT9_002305"
     CDS             complement(12128..13072)
                     /gene="mhpB"
                     /locus_tag="ACHUT9_002305"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFR1348765.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(13074..14738)
                     /locus_tag="ACHUT9_002306"
     CDS             complement(13074..14738)
                     /locus_tag="ACHUT9_002306"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFR1348766.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLHVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14780..15609
                     /locus_tag="ACHUT9_002307"
                     /pseudo
     CDS             14780..15609
                     /locus_tag="ACHUT9_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
     gene            15686..16768
                     /gene="lacI"
                     /locus_tag="ACHUT9_002308"
     CDS             15686..16768
                     /gene="lacI"
                     /locus_tag="ACHUT9_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFR1348767.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16891..19965
                     /gene="lacZ"
                     /locus_tag="ACHUT9_002309"
     CDS             16891..19965
                     /gene="lacZ"
                     /locus_tag="ACHUT9_002309"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFR1348768.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            20017..>20281
                     /locus_tag="ACHUT9_002310"
     CDS             20017..>20281
                     /locus_tag="ACHUT9_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1348769.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         4803 a         5545 c         5231 g         4702 t
ORIGIN      
        1 ttcgcattgt atatatcact gtatcattct caatcactcg catattacat tcacataatg
       61 tatataaaca atacttgttc acagaacata ttttttaaat gcaattaatt gacaaactaa
      121 atcacatcta aatacaatgt ataaaacaaa ataactatac ataccaaaca tacaaacact
      181 taacatatga attgcataaa aacattaccc agtcgaacct ccgggtctga aagcccttgc
      241 ttttcagtta ttagcaacat ttaacattta taaattaaca tctggctgcg ccagagatta
      301 cgtctctgag tgcatcgttg tgcttgtagt ataataaata aaatggagtg tcattaagtg
      361 gataaggttt tagattcagc cctcctttct tcggcaaata aaagaaaggg tatccttgct
      421 attggcgcac atcctgacga tatagaatta ggctgtggcg catcgcttgc tcgtcttgcg
      481 caaaaaggaa tttatatcgc agcagtggtg atgactaccg gcaactctgg tacagatgga
      541 ataatcgatc gccatgaaga atcgcgcaac gccctaaaga tattagggtg ccaccaaact
      601 attcatctta attttgctga cacccgcgct catttacagc tcaatgatat gatttccgcc
      661 ctggaagaca tcattaaaaa tcaaattcct tctgatgttg aaatcatgcg ggtatatacc
      721 atgcatgatg ccgaccgcca tcaggatcat ctcgctgttt atcaagcttc aatggttgcc
      781 tgccgcacta ttccacaaat tctcggctac gaaaccccaa gtacctggct ttcatttatg
      841 cctcaggttt ttgaatccgt taaagaagaa tatttcacag tcaaacttgc tgcattaaaa
      901 aaacataaaa gccaggaacg acgtgattat atgcgtcatg atcgcctgcg tgcagttgca
      961 caatttcgcg ggcaacaggt caatagcgat ctgggtgaag gctttgtaat tcataaaatg
     1021 attctttgag caaattgcat gaaaacctgg atatttatct gtatgtccat agcaatgttg
     1081 ctatggtttt taagtacgct aagacgtaaa cccagtcaaa agaaaggctg tattgacgcc
     1141 attatacctg cgtataacga aggcccgtgt ctggcgcagt cactggataa tctactgcgt
     1201 aacccttatt tttgccgggt aatttgcgtt aacgacggct ccacggacaa taccgaagcg
     1261 gtcatggcgg aagtcaaacg caaatggggc gaccgctttg ttgccgtcac gcaaaaaaat
     1321 accggtaaag gtggtgcgct gatgaatggc ctcaattatg ccacctgcga ccaggttttt
     1381 ttaagtgatg ccgacaccta tgttccgccc gatcaagacg gaatgggcta tatgctggca
     1441 gaaattaagc gcggtgctga tgccgtaggc ggcattccct ctactgcgtt gaaaggcgcg
     1501 gggctgttac cgcacatccg cgcgaccgta aagttgccga tgattgttat gaagcgcacg
     1561 ctacagcagc tcctgggcgg cgcaccgttt attatcagcg gtgcctgcgg aatgttccgt
     1621 actgatgtat tgcgtaagtt cggtttctcg gatcgtacta aagtcgaaga ccttgatctc
     1681 acctggacat tagtggcaaa cggctaccgt attcggcagg cgaatcgctg catcgtatac
     1741 ccacaggaat gcaacagtcc gcgtgaggag tggcgtcgct ggcggcgttg gattgtgggc
     1801 tacgcggtct gtatgcgcct gcataaaagg cttttattta gccgcttcgg tatcttcagt
     1861 atatttccta tgctgttggt tgtgatttat ggcgttggga tttatctcac tacctggttt
     1921 aatgaattca tcaccaccgg gccgcatggt gtggtgttgg caatgtttcc gcttatctgg
     1981 atcggcgtag tttgtgttat tggtgctttt agcgcctggt ttcatcgttg ctggttgttg
     2041 gtgcctttag cgccgctttc cgttgtgtat gtattattag cttatgccat ctggattatc
     2101 tatggactta ttgccttttt tactggacgc gaacctcagc gcgacaaacc cacccgctat
     2161 tccgcactgg tggaagcgtc aaccgcttat tcccaacctt ctgtcacagg aactgaaaaa
     2221 ctatctgaag cttaatgtta aagagaaaaa ttattcagat tgcagaccag gtaattattg
     2281 atgaaactgc aggtgaagtt gttatcggcg cgaatacccg tatttgtcat ggtgccgtta
     2341 ttcagggtcc ggtagtgatt ggcgcaaact gcctgatagg taattatgcg tttattcgtc
     2401 ctggcacaat aatcagcaat ggtgtaaaaa ttggttttgc caccgaaatt aaaaatgcgg
     2461 ttattgaagc ggaagcaacg attggtccgc aatgttatat tgccgactcg gtagttgcaa
     2521 accaggcata tttgggcgca caagtacgta ccagtaatca tcgtctggat gaacagcctg
     2581 tgtctgttcg aactccagag ggaattatcg ctaccggatg cgataaatta ggttgttata
     2641 tcgggcagcg ttcacgcctt ggcgtacaag ttattatttt gcctgggcga attatttccc
     2701 cgaacacgca acttggcccg cgcgtgattg tagaacgtaa tttacctacc ggaacttatt
     2761 cactccgaca agaacttatc cgtacaggag attaaaatga taaaacggac gttattagcg
     2821 gcggccattt ttagcgcatt gcccgcttat gccgggttaa cttccattac cgcgggctac
     2881 gattttaccg attattctgg cgatcatggc aaccgtaatt tagcgtatgc tgaactggtg
     2941 gcgaaagttg aaaacgcaac gctgcttttt aatctttccc aggggcgtcg tgattatgaa
     3001 actgaacatt tcaatgccac tcgcggtcag ggtgcggtct ggtataaatg gaataactgg
     3061 ctgacaaccc gaacgggtat tgcctttgcg gataatacgc cggtctttgc ccgccaggat
     3121 tttcgtcagg atattaacct ggccctgttg ccaaaaacgc ttttcacgac cggttatcgc
     3181 tacactaaat attacgatga tgtcgaagtc gatgcctggc aaggcggcgt atcactctat
     3241 actggcccgg tgatcaccag ctaccgctat acccattatg actccagcga tgcaggtggt
     3301 agttatagca atatgatttc cgtgcgtctg aatgacccgc gcggcactgg ttatacgcaa
     3361 ctatggctaa gccgcggaac aggcgcttac acctatgact ggacgccaga aacacgctac
     3421 ggcagcatga agagcgtcag tttgcaacgt attcaaccgc tgactgagca acttaatctc
     3481 gggctgacgg caggtaaagt gtggtacgac accccaaccg atgattataa cggtctgcaa
     3541 cttgcagccc atctgacctg gaaattctga ttgcttctgc cgcccgctat ccggggcggc
     3601 cttccctgcc gattagcccc actccctttc ctcgttgttt tccgaccaca ttcaccggat
     3661 aaattttatt ctccagcgtt atatactata gggaggtatg cattgacata tagcatacct
     3721 ccctatagta tattgcgtgc agatgatgag gtgcgaaatg cccagtactc cggaagagaa
     3781 gaaaaaggtc cttactcgag ttcgtcgtat tcgggggcag attgatgctc tggaacggtc
     3841 gctggagggt gatgccgaat gccgtgccat actccaacag atcgctgccg ttcggggcgc
     3901 ggctaacggg ctgatggcag aagtgcttga aagccatatc cgggaaacgt ttgaccgaaa
     3961 tgactgctac agccgcgaag tcagccaatc cgttgacgac actattgaac tggttcgagc
     4021 ctatcttaaa tagctgaatc tattaccata ttgaggaaga gcgagagatg aaatcacgtg
     4081 ctgccgttgc atttgctccc ggtaaaccgc tggaaatcgt tgaaattgac gttgcaccac
     4141 cgaaaaaagg tgaagtgctg attaaagtca cccataccgg cgtttgccat accgacgcat
     4201 ttaccctctc cggggatgac ccggaaggtg tattcccggt ggttctcggt cacgaagggg
     4261 ccggcgttgt ggttgaagtc ggtgaaggcg taaccagcgt caaacctggc gaccatgtga
     4321 tcccgcttta caccgcggag tgcggcgagt gtgagttctg tcgttctggc aaaactaacc
     4381 tctgtgttgc ggttcgcgaa acccagggta aaggcttgat gccagacggc accacccgtt
     4441 tttcttacaa cgggcagccg ctttatcact acatggggtg ctctacattc agtgaataca
     4501 ccgtggtcgc ggaagtgtct ctggccaaaa ttaatccaga agcaaaccat gaacacgtct
     4561 gcctgctggg ctgtggcgtg accaccggta ttggcgcggt gcacaacaca gctaaagtcc
     4621 agccaggtga ttctgttgcc gtgtttggtc ttggcgcgat tggtctggca gtggttcagg
     4681 gcgcgcgtca ggcgaaagcg ggacggatta tcgctatcga taccaacccg aagaaattcg
     4741 atctggcgcg tcgcttcggt gctaccgact gcattaaccc gaatgactac gacaaaccga
     4801 ttaaagatgt cctgctggat atcaacaaat ggggtatcga ccataccttt gaatgcatcg
     4861 gtaacgtcaa cgtgatgcgt gcggcgctgg aaagtgcgca ccgcggctgg ggtcagtcgg
     4921 tgatcatcgg ggtggcaggt tctggtcagg aaatctccac ccgtccattc cagttggtca
     4981 ctggtcgcgt atggaaaggt tccgcgtttg gcggcgtgaa aggtcgttcc cagttaccgg
     5041 gtatggttga agatgcgatg aaaggtgata tcgatctgga accgtttgtc acgcatacca
     5101 tgagccttga tgaaattaat gacgccttcg acctgatgca tgaaggcaaa tccattcgaa
     5161 ccgtaattcg ttactgattt cccgcaggta taccccgtcc acttcagacg gggttcttaa
     5221 tactctcccc gggcagccgt ccgggggatt aaccgtgaga taatgactga tggaactcat
     5281 tgaaaaacat gccagctttg gcggctggca aaatgtgtat cggcattatt cccaatcact
     5341 gaaatgtgaa atgaatgtcg gcgtctatct cccaccaaaa gccgcgaatg aaaaattgcc
     5401 ggtgctgtac tggctttcag gcctgacctg caacgagcag aatttcatta ctaaatcggg
     5461 gatgcagcgt tacgcggctg agcacaacat tattgttgtt gcgccggaca ccagtccgcg
     5521 aggcagtcat gtcgcagatg ccgaccgtta cgatctcggg caaggtgccg ggttttacct
     5581 gaacgcaacg caagcgccgt ggaatgaaca ttacaaaatg tatgactata tccgcaacga
     5641 gctgccggat ttagtgatgc aacattttcc ggcaacgacc agaaagtcta tctctggtca
     5701 ttctatgggc gggctgggcg cgctggtgct ggcgttacgt aacccagatg aatatgtcag
     5761 cgtctcggcg ttttcgccca ttgtctcccc atcgcaagta ccgtggggac agcaagcctt
     5821 tgccgcatat cttggtgaaa ataaagatgc ctggttggat tacgatccgg tgagtcttat
     5881 ttcacaaggt caacgcgttg cggaaatcat ggttgatcag gggttgagtg atgattttta
     5941 cgcggaacag ctgcggactc caaatcttga aaagatctgc caggagatga atatcaagac
     6001 gttaatccgt tatcacgagg gttacgacca cagctattat tttgtctcca gttttattgg
     6061 cgagcatatt gcctaccacg ccaataaact gaatatgcgt tgataatagt gcacgactgc
     6121 cggatgcggc gtgaacgcct tatccggcct acacttcgcc cgtaaaccgt aggcctgata
     6181 agacgcgcaa agcgtcgcat caggcatggc gcacgactgc cggatgcggc gtgaacgcct
     6241 tatccggtct acacttcgcc cataaaccgt aggcctgata agacgcgcat agcgtcgcat
     6301 caggcatctg cgcacgactt atctatgttt accacatcaa atcatcaggc actttgaagt
     6361 cggcatacgg atcgtcttca tcctgctctt cggcactgag cgcgctgtgc aacacaatac
     6421 tgctggcatc acgctgagca attttatcgg caacactggc gggaataatg gtgtattcac
     6481 cttcgctatt gttatcgacc aacaagcggg caatcgccag acgaccatta atcaactgcg
     6541 cctgtgtgag cttatcgaca aaaatcttct taatcagatt gccgtcagtg aagttaaaac
     6601 caatatcgcc attggcaatg gtgattcggt tcatttcaat gagctgcttc acctgggctt
     6661 tatattcttt cgccaacgcc gcttgttttt gctgttcgct aagctgttta tcacgctcaa
     6721 gctgtgcctt tttattttct tctaccgcag cccgagcttc acgcgcctga acgcgcgatt
     6781 ttttcgccgt tctctcgacc ttcgccgctt ttttgctggt gactaatcct gctttgagca
     6841 actgctcttg taaggtaagt tttgccattt ttgtttccaa acccgctgaa taatttttag
     6901 cgagtatacc tgtaaacgcc gtgggtgtat tgggggcaag tccctttcgc ccctgcttaa
     6961 taaggcgcgt caggcatcgg cacacggctg cattcttgat ctccgactca tcaaaataaa
     7021 caccgccaac ccagcaacaa gaatacccgg tgcagaagcg gccattacgc cgaccgtgcc
     7081 agtgcctaat gccagcattt tcccggccag taacggaccg ctcatagccc ccagacgccc
     7141 taccgccacg gctgttccca cacctgttgc gcggatctgc gaactgtaaa acaacggtgc
     7201 cagggcatac aaaacgcttt gcccacctgt cgcaaacaac cccgcgacaa atcccgccag
     7261 caacataccg ttaaatgacg acaccgttcc aagcgccagc agcgaagcta acatgccgct
     7321 ataaatcagt agcgacatgg ttactggacg cagcttatcc atcaatgcgc ccaacattaa
     7381 cgtcccgctt gccgccccca tttgcagggc aaacatcacc cctgccgcct gcgatggctg
     7441 gaatccttgc tccaccaaaa gtagcggtag ccagttgatc aacatataaa ccaccagcag
     7501 agtgaagaaa taacacaacc acagcagcag cgtcgcggtt gccgtttctg gcgcaaataa
     7561 ggcacgcagt ggtggcgcag cctgtttttc gccagcgaaa accgctgact ccggcagcca
     7621 gcgcattaat agcggcacca gaatcaacgg caccacacca cctacccaaa acaccgtttg
     7681 ccatgctaag tttgcccccg cgaaacccag tgtcgccgcc agcgccgcgc caatgggaac
     7741 accgcaatac atcaggctca ctgccgtccc acgaaaacgt ggacccgcgg cttcagatgt
     7801 cagggcgata agattcggca acgccgcccc cagcccgaca ccggtcatca gccgcgcaaa
     7861 gaccagtgag gggaaatccc aggcaatcgc cgttgccagt gagaacaaac caaacagcgc
     7921 aactgagcca atcaaaatac gcttacgacc ataacggtct gccagcatcc cgccaaccaa
     7981 cgcgccgggt agcaaaccga gtattccggc gctaaatatc cagcccattt gcattttatc
     8041 gagtgcgaaa gcctgggcga tgccacccgc cgcaatgcca gccgcctgaa gatccagccc
     8101 ttccatcaga gcgaccaaaa aacaaagccc gatggtcagc atcaggcggg atgaagatga
     8161 tgaaggggta cgagtcgaca taagatgaac ctcattgcga gtgcaggtga atcgcgactg
     8221 atacattgcc agccgcgtaa tcagaactat ttaatgactg cctacgggcg aaatgtagcg
     8281 aaatgtaggc cggataaggc gttcacgccg catccggcag tcgtgcgcag atgcctgatg
     8341 cgacgctaac gcgtcttatc agacctacaa attctcacgc catccgtagg ccggacaagg
     8401 cgttcacgcc gcatccggct gtcgtgctgg gatgggcata caaaatgcac gttatcggca
     8461 gtcatcatta tttgttgttg cgcagatcca gcgccacgtc gacgatcata tcctcctggc
     8521 cgccaaccat ccggcgtttg cccagctcaa cgagaatatc caccgcactt aagccataac
     8581 gcgccgccgc cgtttcacag tgacgcagga agctcaagta aacaccagcg tatcccagcg
     8641 ccagcgtttc gcgatcgact cgtaccggac gatcctgcaa cggacgcacc aggtcgtcgg
     8701 cggcatccat taacgcatag agatcggtcc catgctgcca gcccagttta tccgcggcgg
     8761 caataaacac ttccagcggt gcgttacctg cgcccgcgcc cattcccgcg aggctggcgt
     8821 cgattcggtc gcagccctct tccaccgccg cgatagagtt cgccacgcca agactcaggt
     8881 tatggtgagc gtgcatgccg gtttgcgttt ctggtttcaa cactgctttc agggcgcgga
     8941 aacggtcacg gatatcgctc atgttcatcg caccgccaga atccaccaca taaatacagg
     9001 tcgcaccgta gccttccatc agctttgcct gcttggcgag attctccggc gtggtcatat
     9061 ggctcatcat cagaaaacca acggtgtcca ttccgagctc gcgggcatac tgaatatgct
     9121 gggcggaaac atcagcttcg gtacagtgcg ttgccacacg aaccacccgc gcgccagcct
     9181 gccaggcatt tttcagatcg tgaatagtgc cgattcctgg cagcaacaac gtcgcgattt
     9241 tggcgtgcct caccacatcc gccgccgctt caatccattc aaggtcgcta tgtgcgccga
     9301 aaccatagtt aaagctggaa ccttgcaaac cgtcgccgtg ggccacttca atcgaatcca
     9361 cgcgggcatc gtccagtgct ttggcaatct ggcgaacgtt ttccagcgaa tactgatgac
     9421 gaatggcgtg cataccgtca cgcaatgtga cgtccgagat ataaagtttt ttaccgttca
     9481 tgccgcttct cctgccttgc gcgccagtga ctgggccatt ttttccgctg tcgccagcgc
     9541 actggaagtc ataatatcga ggttgcccgc ataggcaggc agataatgcg ctgcgccttc
     9601 gacttccagc cagaccgctg ttttcagtcc ggagaattgc cccacgcccg gtaaattgac
     9661 cggtttatcc tgcgggataa cttcaaactg cacgcgctgt ttcaggcgat aacccggtac
     9721 gtaagcctgc accgcctcag ccatttcatt gattgaggct tcgatatcat cttgtgaagc
     9781 ttcgtcgctc aatacataca ccgtgtcacg catcatcaac ggtggctctg ctgggttaag
     9841 cacaataatc gccttccctt ttgccgcgcc gcccaccact tcaatggctc gggaagtggt
     9901 ttccgtaaat tcatcgatat tggcacgcgt gccaggtccg gcagatttac tggcgataga
     9961 agcgataatt tcggcgtaat gaacacgcgc cacgcgtgaa actgccgcca ccattggaat
    10021 ggtggcctgg ccgccgcagg tgaccatgtt gacgttcagt tgatcgacgt tcgcctcgag
    10081 gttaaccacc ggcacgcagt aagggccgat ggcagcaggc gtcaggtcaa ttaagcgaat
    10141 atccggtttc gcttcgcgta aagcggcatc gtttttcaca tgcgcgcccg cgctggtcgc
    10201 atcaaataca atgtcgatat cagcaaattc aggcatgttc atcagcccga tcaccccttc
    10261 atgggtggtg gcgacgccca tacgtctggc acgcgccaga ccgtcggact gaggatcaat
    10321 gccgaccatc accgccatct ccagatgctg accgtgacgc aaaattttaa tcatcagatc
    10381 ggtgccaatg ttgccagaac cgataatggc gactttacgc ttactcatga cagacttcct
    10441 tttggggctg cgcttgaaaa tgtcgcagca actgaaccta tgccttcaat atgggcttca
    10501 aaacgatcgc ccgcattcac cgccaccatc gggcctaatg ccccggtaag aatgatatct
    10561 ccggcgcgca gcggttcacc cagactggcc attttgcgtg ccagccagac ggcggcatta
    10621 agcggatgtc ccaggcattc gctgccgcgc ccgctggaaa cctcttcgtt attacgcgtc
    10681 atcttcatgg cgcagttttt caggtctaac cccgccggac gttgcgccgg accgccgatg
    10741 acatacaccc cgcaggaagc gttatctgcc acggtatcga caaactgaat cgaccagtcg
    10801 cgaatgcggc tccccaccac ttccagcgcc ggaagtaccc attcaatggc gttatacagt
    10861 tcgtcgaagg tgatatcagt tgcgggtaaa tcgcggttca acaccagtgc gatctccgct
    10921 tcaatgcggg gttgcagaac acgggaaaaa ggaatgattt cgttatcgcc ataacacatg
    10981 tcggcaaata acgtcccaaa atccggttga tcaacgccca gttgttgttg cactttcgga
    11041 tgcgtcaggc ccactttacg ccctaccacg cgacgcccct gcacaacgtc atattgcaca
    11101 tttatgtgct gaatggcgta ggccgcttca gcgtgatcga taccaatcag atcgcgcagc
    11161 ggcgcaatcg cttcgccctg ctctgcggcg cggcgtaaat ccgccgccag ttgctcaaga
    11221 gtatgcttcg tcatgaccat tccttaaggg cgtgcgagga aattcagcac caattgattg
    11281 aaagcgtcgg catgttccca ctgcgcccag tgaccacagt cgcggaagat atgcagttca
    11341 gaaccggcaa tgccggacag cagacgcaga cccgcatcca tcggcacaaa gcggtcgttg
    11401 cgcccccaga caatcagggt ttgcgctttg atttccgcca gacgtgggcc aaaatccggg
    11461 aactgtttcg gattagcttc caggctctta acgaagtttt ccaggtgatc gcggcgcgac
    11521 agcatattat tcaggcgcgc ttcaaacagg gcgtcggtca aatcgctggt atcgaaaacg
    11581 aagatatcca tcatcagctt caggttttcg atagtcggct gacgataaag ctgattcagt
    11641 cgcttaatac cttcggttgg catcggcgta aacaaactca tgccgcccgt accgccgccc
    11701 atcagcacca gtttgccgac gcgctccggc cagttaaggg tgaacgccac agaactatgg
    11761 ccgcccatcg agttgcccag caggtggatt ttggcgatat ccagttgatc caccacgctt
    11821 ttcaggattc gtgcattaag atccgatcgt gaaccactat taacgatcga atcgcttttg
    11881 ccccaacccg gacaatccag caggatcacc cgatagcccg cctctaccag cggatcgata
    11941 ttgcggctga agttcgccca gccagtagca cccgggccgg aaccatgcag caggacaacg
    12001 gtttcgtcgc cttgtccgca gtcattaaaa tggatgcgca gcgttttacc cgcttcttct
    12061 acattcagaa aacggctggt ggcggcttcg gtttgtggct gataactcat catcttctcc
    12121 tgcatattca gttctctgtt ctggcgctta acgagccaaa tccggcaatc cactccggga
    12181 ttgggcggta ataacgccct tcgctacgcc agttgccaaa tgcagaaata gcggcaaaag
    12241 cggcgaccca ggttttgatt tcatgtgtcg actttccggc aatggcggaa agctcttcgt
    12301 tactgacggc atccagttcc tgtatgcgtc cctgctccag caaagtcatg aactggttat
    12361 cccaaatcgg gttgagcgga tgcagagttc tctgatcctc aacaaacttc tcagcggcgc
    12421 taatcaccct ttgctgacgc aattcgcgct cactggcggg taaatctttc ccgctcccca
    12481 acagacggtc gcgcatatgg gcatcggctt tcgccagttc gggcaccggc ggctgatggg
    12541 aaagcccacc ggaacccagg aacagcacgc gtttattgag agtgctggtg aaacgtccaa
    12601 tggcttcacc caacatgcgg gtacgctgga aaccgggcag cggcgtggcg acaccgttga
    12661 tgaacacagg cagaactggc accttatcca gcccaccgag caggaactcc agcggctggg
    12721 cgaacccgtg gtccacctgc atacagtaag aaacggcaag atcgatcccg ctcttcatga
    12781 cagcatgcgc acaggcctcc gccagctcca caggcacggg cagctctcct gccgcactgc
    12841 cgaaatcacc aattgccgtc gctccaacgc ctaaacagaa cggtggcatc acgtcataga
    12901 aaaagccgtt gtagtgatct ggcgcaaaca gcaccaccag ttcaggggag aaagccgcaa
    12961 tacgctcgcg ggcgctggca atcacgccat tgacctcatc gagtacctct tgcgccgggt
    13021 cgacataccc caccaacggc gagtgggaaa gacagtgaag ataagcgtgc atatcaggct
    13081 accttttcga cagaaacgtc ggcatcaggg cgggtcagcg tcatcaccga cgccagttta
    13141 ttcagcgtat tgcccagggt ttgcggaatg gcggtggcgg caacaaagcg atccgggcgc
    13201 atcaccacca gcgaagcatt gtgttgcgcg aaccagctac gcagacgacc ttgcgtatcg
    13261 cccacgtgta gcacgccgtc gtggttatcc tgtgcggtat gaatttgcac ttccggcacc
    13321 acctgaatga agcgtgtgcc caacgcgcgc cactgctgga tttgctcgtc gctcatcccc
    13381 cacagtggat tgcatcccca gccaattacc gcgaagttcg cgccgatcgc gttatcgagc
    13441 agcgtcacgt cgccgttttc cagcgtgact ttcggctgaa taaacatctt gccgaccgga
    13501 gagtgcttcg cttcgccctc acgcaccagc gcaccgccgt aatattgcgg catcggcttg
    13561 aagcgcattt cgaggaagta gcgttttact ggcggcagat aattcaacag ccaggaaacg
    13621 ccgtcacgta acgtaccctg ccagcgtttc ggcggagcca gcacgttgcc cgccgtcacg
    13681 gacaggtcaa tcatcgcttt ggcgtgatcg cgacgttctt gttgataggt atcgagcagc
    13741 gcatcgcggg ctttcccctg gataaccaac gccagtttcc aggcgaggtt aaaggcgtcg
    13801 cgcataccac tgttatagcc ctgcccctgc cacaccggca tgatgtgcgc ggcatcgccc
    13861 gccagcagta cgcgatcaat acggaaacgt tgcgccagtc gcgcgttatg ggtgtagaca
    13921 cgctggcgaa tcaattcaac attgtccgga ttaggcagca ctttgcttaa cagcttgcgc
    13981 atattttgcg gctcacgcag ctgctcttcg gtttctcccg gcatcaccat aaattcaaag
    14041 cgacgtaccg catgaggcag cgcggcagaa acatacgggc gcaccggatc gcaacacaaa
    14101 tagatatgcg gcgtacttaa cggatcgttg gcgatatcta ccacaatcca ctgatttggc
    14161 gcagttttac cttcaaacgg cacattcagg gtgcgacgaa caaaacttgc cccaccatca
    14221 caggccacca gccactgggc tttgatcgtt tcccgctgcc cttctgccgt tttcaggtgc
    14281 aaggtcactt cgtcatcttg ctggctgaag gcctccagct cgcgggaaaa caagcagcgc
    14341 acattcggaa aacgcgacaa cccttccagc atcaccgcat cgacctgtgg ctgaataaag
    14401 gcgttacggc gcggccagcc aaattcatcg gtcattggct gaatatcagc aaaacagcgg
    14461 ccttttgggg tgagaaaacg catcgcgtgc cacggtgtag tgtgcggcag aacattctcg
    14521 accaggccga ccgactgcat ggtgcgcagc gcctcgtcat caataccaat cgcacgcggg
    14581 tagtcgatca acttatcgag tttctccacc accagcacgt caatgcccat ctgaccgaga
    14641 tagttcgcca tcatcagccc aaccggaccg gcaccagcga tcgccacctg aacgctatgg
    14701 ttaacagcag gctggatgtc agggtgttgt attgccattt ctgcacaacg ttgttttaac
    14761 tatagaaatg tcaattaata tgcagaacaa tgagcagacg gaatacaaaa ccgtgcgcgg
    14821 cttaacccgc ggtctaatgt tattaaatat gttaaataaa cttgatggcg gtgccagcgt
    14881 cgggctgctg gcggaactca gcggcctgca tcgcaccact gtgcggcgac tgctggagac
    14941 gctgcaggaa gagggatatg tccgccgtag cccctccgat gacagttttc gtctgaccat
    15001 caaagtgcgg caattaagcg aaggatttcg tgacgaacag tggatttctg cactggcggc
    15061 cccgctgctg ggcgatctgt tgcgcgaagt ggtatgacca acagatgtgt ccaccctgga
    15121 tgttgatgca atggtggtac gcgaaaccac ccaccgtttc agccgcttat cctttcaccg
    15181 ggcaatggtc gggcgacgtt taccgctcct gaaaaccgcc tcgggcctga cctggctggc
    15241 cttttgcccg gaacaagaac gcaaggaatt aatcgaaatg ttagccgccc gccccggtga
    15301 tgactatcaa ctggcacgag aaccgttaaa gctgcaagcc attctggcgc gcgcaaagag
    15361 ggttacggac agaactaccg cggctgggat caggaggaga agatcgcctc tatcgccgta
    15421 ccgctgcgca gtgaacaacg ggtgattggc tgtctgaatc tggtgtatat ggcgagcgca
    15481 atgaccattg aacaggcagc ggaaaagcat cttccggcgc tacaacgggt agcaaaacag
    15541 atcgaagaag gggttgaatc gcaggctatt ctggtggccg gacggcgaag cggcgtgcat
    15601 ttacgttgac accatcgaat ggcgcaaaac ctttcgtggt atggcatgat agcgcccgga
    15661 agagagtcaa ttcagggtgg tgaatgtgaa accagtaacg ctatacgatg tcgcagagta
    15721 tgccggtgtc tcttatcaga ccgtttctcg cgtggtgaac caggccagcc acgtttctgc
    15781 gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc ccaaccgcgt
    15841 ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct ccagtctggc
    15901 cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc
    15961 cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca
    16021 caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg atgaccagga
    16081 tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg atgtctctga
    16141 ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac tgggcgtgga
    16201 gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat taagttctgt
    16261 ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc aaattcagcc
    16321 gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa ccatgcaaat
    16381 gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga tggcgctggg
    16441 cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct cggtagtggg
    16501 atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca tcaaacagga
    16561 ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc agggccaggc
    16621 ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
    16681 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    16741 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    16801 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt gaaattgtga
    16861 gcggataaca atttcacaca ggaaacagct atgaccatga ttacggattc actggccgtc
    16921 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
    16981 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
    17041 cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg
    17101 ccggaaagct ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac
    17161 tggcagatgc acggttacga tgcgcccatc tacaccaacg tgacctatcc cattacggtc
    17221 aatccgcctt ttgttcccgc ggagaatccg acgggttgtt actcgctcac atttaatatt
    17281 gatgaaagct ggctacagga aggccagacg cgaattattt ttgatggtgt taactcggcg
    17341 tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc aggacagccg tttgccgtct
    17401 gaatttgacc tgagcgcatt tttacgcgcc ggaaaaaacc gcctcgcggt gatggtgctg
    17461 cgctggagtg acggtagtta tctggaagat caggatatgt ggcgaatgag cggcattttc
    17521 cgtgacgtct cgttgctgca caaaccgagc acacaaatca gcgatttcca tgttgccact
    17581 cactttaatg atgatttcag ccgcgcggta ctggaggcag aagttcagat gtacggcgag
    17641 ctgcgcgatg agctgcgggt gacggtttct ttgtggcagg gtgaaacgca ggtcgccagc
    17701 ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc
    17761 acgctacgtc tgaacgtcga aaacccggcg ctgtggagcg ccgaaatccc gaatctctat
    17821 cgtgcggtgg ttgaactgca caccgacgac ggcacgctga ttgaagcaga agcctgcgat
    17881 gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg
    17941 ctgattcgcg gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag
    18001 cagacgatgg tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgttgt
    18061 tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg accgcttcgg cttgtacgtg
    18121 gtggatgaag ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat
    18181 ccgcgctggt tacccgcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat
    18241 cacccgagtg tgatcatctg gtcgctgggg aatgagtcag gccacggcgc taatcacgac
    18301 gcactctatc gctggatcaa atctgtcgat ccatcccgcc cggtgcagta tgaaggcggc
    18361 ggagccgaca ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac
    18421 cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag
    18481 acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggcttc
    18541 gctaaatact ggcaggcgtt tcgtcagtac ccccgtttac agggcggctt cgtctgggac
    18601 tgggtggatc agtcgctgat taaatatgat gaaaatggca atccgtggtc ggcttacggc
    18661 ggtgattttg gcgatacacc gaacgatcgc cagttctgca tgaacggtct ggtctttgcc
    18721 gaccgcacgc cgcatccagc gctgacagaa gcaaaacacc agcagcagtt tttccagttc
    18781 cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac
    18841 gagcttctgc actggtcggt ggcgttggat ggcaagccgc tggcaagcgg tgaaatgcct
    18901 ctggatgttg ctccacaaga taaacagttg attgaactgc ctgaactacc gcagccggag
    18961 agcgccggac aactctggct tacggtacac gtagtgcaac cgaacgcgac cgcatggtca
    19021 gaagccggac acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagcgtgaca
    19081 ctccccgccg cgccccacgc catcccgcaa ctgaccacca gcgaaacgga tttttgcatc
    19141 gagctgggta ataaacgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg
    19201 attggcgctg aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg
    19261 gataacgaca ttggcgtaag tgaagcgacc cgcattgacc cgaacgcctg ggtcgaacgc
    19321 tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca
    19381 cttgccgacg cggtgctgat tacgaccgct cacgcgtggc agcatcaggg aaaaacctta
    19441 tttatcagcc ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat
    19501 gttgaagtag cgagcgatac accgcatccg gctcggattg gcctgacctg ccagctggcg
    19561 caggtagcag aacgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc
    19621 cttactgccg cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac
    19681 gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac
    19741 cagtggcgcg gcgacttcca gttcaatatc agtcgctaca gccaacaaca actgatggaa
    19801 accagccatc gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc
    19861 catatgggga ttggtggcga cgactcctgg agcccgtcag tttcagcgga attccagctt
    19921 agcgccggta gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg
    19981 ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa aaacacaaac
    20041 ttttggatgt tcggtttatt ctttttcttt tactttttta tcatgggggc ctacttcccg
    20101 tttttcccga tttggctaca tgacatcaac catatcagca aaagtgatac gggtattatt
    20161 tttgccgcta tttctctgtt ctcgctatta ttccaaccgc tgtttggtct gctttctgac
    20221 aaactcgggc tgcgcaaata cctgctgtgg attattaccg gcatgttagt gatgtttgcg
    20281 c
//
LOCUS       DAZPEP010000065        20240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZPEP010000065 DAZPEP010000000
VERSION     DAZPEP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.065"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..70)
                     /locus_tag="ACHUT9_002311"
     CDS             complement(<1..70)
                     /locus_tag="ACHUT9_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348770.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLK"
     gene            689..1459
                     /locus_tag="ACHUT9_002312"
     CDS             689..1459
                     /locus_tag="ACHUT9_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFR1348771.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1450..1923
                     /locus_tag="ACHUT9_002313"
     CDS             1450..1923
                     /locus_tag="ACHUT9_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFR1348772.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHUT9_002314"
     CDS             2031..2570
                     /gene="dnaT"
                     /locus_tag="ACHUT9_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFR1348773.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHUT9_002315"
     CDS             2573..3310
                     /gene="dnaC"
                     /locus_tag="ACHUT9_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1348774.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHUT9_002316"
     CDS             3359..3853
                     /gene="yjjA"
                     /locus_tag="ACHUT9_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFR1348775.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            4107..6398
                     /gene="opgB"
                     /locus_tag="ACHUT9_002317"
     CDS             4107..6398
                     /gene="opgB"
                     /locus_tag="ACHUT9_002317"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFR1348776.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHUT9_002318"
     CDS             complement(6537..7559)
                     /gene="lgoD"
                     /locus_tag="ACHUT9_002318"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFR1348777.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7698..8612
                     /locus_tag="ACHUT9_002319"
     CDS             7698..8612
                     /locus_tag="ACHUT9_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFR1348778.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8827..10188
                     /locus_tag="ACHUT9_002320"
     CDS             8827..10188
                     /locus_tag="ACHUT9_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1348779.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10237..11892)
                     /gene="tsr"
                     /locus_tag="ACHUT9_002321"
                     /pseudo
     CDS             complement(10237..11892)
                     /gene="tsr"
                     /locus_tag="ACHUT9_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(12011..12457)
                     /gene="hpaR"
                     /locus_tag="ACHUT9_002322"
     CDS             complement(12011..12457)
                     /gene="hpaR"
                     /locus_tag="ACHUT9_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFR1348780.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12729..14018
                     /gene="hpaG"
                     /locus_tag="ACHUT9_002323"
     CDS             12729..14018
                     /gene="hpaG"
                     /locus_tag="ACHUT9_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFR1348781.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            14015..15481
                     /gene="hpaE"
                     /locus_tag="ACHUT9_002324"
     CDS             14015..15481
                     /gene="hpaE"
                     /locus_tag="ACHUT9_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1348782.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15483..16334
                     /gene="hpaD"
                     /locus_tag="ACHUT9_002325"
     CDS             15483..16334
                     /gene="hpaD"
                     /locus_tag="ACHUT9_002325"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFR1348783.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16344..16724
                     /locus_tag="ACHUT9_002326"
     CDS             16344..16724
                     /locus_tag="ACHUT9_002326"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFR1348784.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16835..17638
                     /gene="hpaH"
                     /locus_tag="ACHUT9_002327"
     CDS             16835..17638
                     /gene="hpaH"
                     /locus_tag="ACHUT9_002327"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFR1348785.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17649..18437
                     /gene="hpaI"
                     /locus_tag="ACHUT9_002328"
     CDS             17649..18437
                     /gene="hpaI"
                     /locus_tag="ACHUT9_002328"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFR1348786.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18612..19988
                     /gene="hpaX"
                     /locus_tag="ACHUT9_002329"
     CDS             18612..19988
                     /gene="hpaX"
                     /locus_tag="ACHUT9_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFR1348787.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19998..>20240
                     /locus_tag="ACHUT9_002330"
     CDS             19998..>20240
                     /locus_tag="ACHUT9_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFR1348788.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4871 a         5432 c         5171 g         4766 t
ORIGIN      
        1 cttttaaccc tgcttgcata acaggtattt tactgataac aattccattt ttgcagcatc
       61 ctggcaacat atttcctcca catccactgt ctaaaaatca tgatggttta ttcaggatat
      121 tcattcctta aatgtttcca gcaaattaat agattaagcg aataaattat ccttagcatt
      181 cactttcata tcagcatact cctgctgcac aacaactcca ctgcatgtgt cagaaggtta
      241 gcatcaaatg ataacctggt gaaatagcct ggagacattt aaaatatcta cattaagttg
      301 ttaggaatac ttaatgaaaa acagaaatca cattcattta atttataatt aaatgatatc
      361 ccttcccgca gaatgaaaaa tcacacaacc aatatttatt caatgaaatc tgatggtttt
      421 aacttgttaa cattaaaaaa cgtgcccggt tatctattat cctgattcgt aaaggccaaa
      481 caataaattc attatagtga taatattcat gtgatagttt gtttgcggcg agagataatt
      541 cgctttttat caccgagctt gtcccgataa ccaggattaa tcagcgcaat tttgatctta
      601 acctaccgca aaacaaccgt gcttcatgat acaaatggat tctcattatc tgaacaatac
      661 gcagcacgtc tatgacaaag ggcgagttat gcaaactgag caacagcgag ccgtaacacg
      721 gctttgtatc cagtgtggat tatttctttt gcaacatggt gcggaaagcg cgttggttga
      781 tgagctttcc tcacgactgg gtcgggcgtt ggggatggac agcgtcgaaa gttctatctc
      841 ttcgaacgcc atagtgctga caaccattaa agatgggcaa tgcctgacat cgacacgtaa
      901 aaatcacgat cgcggcatta atatgcatgt ggtgactgaa gtccagcaca ttgtgattct
      961 ggcggagcac catctgctgg attacaaagg cgtagagaaa cgatttagcc aaattcagcc
     1021 attacgttac ccaagatggc tggtagcctt aatggttggc ctttcttgcg cctgtttctg
     1081 caaactcaat aaaggtggct gggatggtgc cgtcatcacc ttctttgcca gtacggccgc
     1141 gatgtatatc cgccagctgc tggcacaacg tcatcttcat ccgcagatca acttttgcct
     1201 caccgctttc gccgccacca ccatttccgg attgcttttg caacttccca ctttcagcaa
     1261 tacacccact atcgcgatgg ccgccagcgt tctgctgctg gtgccgggat ttccgttgat
     1321 taatgccgtc gccgatatgt ttaaaggcca catcaatacc ggactggcac gctgggcgat
     1381 cgccagtctg ctgacactgg ctacctgcgt cggcgtggtg atggcactga cgatttgggg
     1441 gctacgcgga tgggtgtgat cgagtttctg tttgcattgg cgcaggatat gatcctcgcc
     1501 gccattccag cggtcggctt tgcgatggtg ttcaacgttc ccgttcgggc attacgctgg
     1561 tgtgcgctgc ttggcgcgat tggtcatggt tcacgaatga tcttaatgac cagcgggctg
     1621 aatattgagt ggtcaacctt tatggcctcc atgctggtcg gcaccattgg tattcaatgg
     1681 tcgcgctggt atctggcgca tccgaaagtg tttaccgtgg cggccgttat ccctatgttc
     1741 ccgggcatat cggcttatac cgcaatgatt tcggcggtaa aaatcagcca gttaggttac
     1801 agcgaaccgt tgatgattac cctgttaacc aactttctta cagcttcatc gattgttggt
     1861 gcgttatcca tcggtctttc cattcctgga ttatggttgt accgcaagcg ccctcgcgta
     1921 taaaattgcc tcgcttaaag cacacggatg agagacagcc tcctctcctc cgtgtgttac
     1981 tataaaagtt atctcccttc tcgttcatcg ttccatattt gagaaacagt atgtcttcca
     2041 gagttttgac cccggacgtc gttggtattg acgccctggt acacgatcac caaaccgttc
     2101 tggcaaaagc tgaaggcggt gtggttgccg tatttgctaa caatgccccg gcgttttatg
     2161 ccgtcacgcc tgcacgcctg gctgaactgc tggcgctgga agaaaagctg gcgcgtccgg
     2221 gaagcgatgt cgctctggac gatcaactct atcaggaacc gcaagccgct cccgttgctg
     2281 tacccatggg gaaattcgcc atgtatccgg actggcaacc cgatgccgat tttatccgcc
     2341 tggcggcgct atggggtgtg gcactaagag agccggtgac caccgaagaa ctggcctcat
     2401 tcattgccta ctggcaggcg gaaggtaaag tctttcacca tgtgcagtgg caacaaaaac
     2461 tggcgcgcag cctgcaaatc ggtcgtgcca gcaacggcgg actgccgaaa cgagatgtga
     2521 atacggtcag cgaacctgac agtcaaattc caccaggatt cagagggtaa cgatgaaaaa
     2581 cgttggcgac ctgatgcaac gcctgcaaaa aatgatgcct gcccatatca aacctgcatt
     2641 caaaacgggt gaagaactac tggcctggca gaaagaacaa ggagcgatcc gctccgccgc
     2701 tctcgaacgt gaaaatcggg cgatgaaaat gcagcgcacc tttaaccgct ccggtattcg
     2761 gccactgcat cagaactgct cctttgagaa ctatcgcgtt gagtgtgaag ggcagatgaa
     2821 tgcgttaagc aaagcgcgcc agtacgtcga agagtttgac ggcaacatcg ccagcttcat
     2881 cttttctggt aagccaggaa ccggcaaaaa ccatctggcg gcggcaatct gcaacgagct
     2941 gctgctacgc ggtaaatccg tattgatcat caccgtggcc gatattatgt cggcgatgaa
     3001 agataccttc aggaatagcg gtaccagtga agaacaactg cttaacgatc tgagcaacgt
     3061 tgatctgctg gtgatcgatg agatcggcgt gcagaccgaa tcgaaatacg aaaaagtgat
     3121 catcaaccag atcgtcgatc gccgctcttc ttccaaacgc ccaaccggga tgctgaccaa
     3181 cagtaatatg gaagagatga ccaagttgct gggcgaacgc gtgatggacc gtatgcgcct
     3241 gggtaacagt ttgtgggtga tcttcaactg ggatagctac cgtagccggg taacaggtaa
     3301 agagtattaa tccccgtttc tggcggtata ctgcgacaca attccacctt gagtaatgat
     3361 gatgaaaact gttaaacatc ttctgtgctg tgccattgcc gccagcgcat taatttccac
     3421 cggggtgcat gctgcgtcct ggaaagatgc gctctccagc gccgccagcg aacttggcaa
     3481 ccaaaacagc acgacacagg aaggcggttg gtcgctcgcg tcattaacta acttgcttag
     3541 cagcgggaac caggccttaa gcgcagataa catgaacaac gccgcaggca ttctgcaata
     3601 ctgcgcgaag caaaagctgg cgtcggtaac cgatgccgaa aacatcaaga accaggtgct
     3661 ggaaaagctg ggcctgaaca gtgaagagca aaaagaagac accaactatc tggatggcat
     3721 tcagggtttg ctgaaaacca aagatggtca gcaactcaat ctggataaca tcggaacgac
     3781 tccgctggca gaaaaggtga aaaccaaagc ctgcgatctg gtgttaaagc aggggctgaa
     3841 cttcatttcc tgaaccgcga aacgccgcgt tttagctgtt acccggctga cgcggctctc
     3901 tcctgccctt ttccctgctc ctggacggtt ttccccctgt tacacgggaa gcgaccgcga
     3961 tcaaaaaagt ctgtttataa actaattcct aacaacatcg catttttttg acactagaat
     4021 tgcagcaata tggcgacgcc attacattgt gcttctcaaa aagcttaaac gttggctaaa
     4081 cggcccggtt tagtgaggat cgcccgttgt cagaactact ctctttcgcc ctttttctcg
     4141 cctctgtgct gatttacgca tggaaagcgg gacgtaacac ctggtggttt gcagccacat
     4201 taacggtgct ggggctattt gtcgttttaa atatcaccct gtttgccagc gactatttta
     4261 ctggcgatgg tattaacgac gcggttctct ataccttaac caacagcctg accggtgctg
     4321 gcgtcagcaa atacatcctg ccgggtatcg gcattgtgct ggggctgaca gcggtgttcg
     4381 gtgcgctggg ctggatcctg cgccgtcgtc gccatcatcc gcaccatttt ggttacagcc
     4441 tgctggcgct cttactggcg ctgggttcgg tggacgccag cccggcattt cgtcagataa
     4501 cggaactggt gaaatcccag tcacgcgatg gcgacccgga ctttgcggct tattataaag
     4561 agccgtcgaa aactatccct gacccgaaac tcaacctggt ttatatctac ggcgaaagtc
     4621 tcgagcggac ctattttgat aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt
     4681 tgaaaaatga aggcctggat ttcagccaca cgcagcagct gccaggaacg gattacacga
     4741 ttgcgggcat ggtggcttct cagtgcggca taccgctgtt tgcccccttt gaaggcaacg
     4801 cctccgcctc tgtctccagc ttcttcccgc agaacatctg tctgggcgat atcctgaaaa
     4861 actcgggtta tcagaactat ttcgtgcagg gcgcgaatct gcgttttgcc ggtaaagatg
     4921 tgttcctgaa gtcgcatggc ttcgaccact tatacggctc agaagagctg aaaagcgtgg
     4981 tggccgaccc gcactatcgc aacgactggg gattctacga cgataccgtt ctcgatgaag
     5041 cgtggaaaaa gtttgaagag ctttcccgct caggtcagcg attctcactg tttaccctga
     5101 cagtcgatac ccatcacccg gatggtttta tctcgcgtac ctgtaaccgc aaaaaatatg
     5161 attttgacgg taagccgaac cagtcattca gcgcggtaag ttgcagccag gagaatatcg
     5221 cggcgtttat caacaaaatc aaagcgtcac cgtggtttaa agataccgtc atcgtcgtct
     5281 cttctgacca tttagcgatg aacaacacgg cgtggaaata cctcaataag caggatcgca
     5341 ataacctgtt ttttgtcatt cgtggcgaca agccgcagca agagacgctg gcagtgaagc
     5401 gtaacacgat ggataacggc gcgacggtgc tggacattct cggtggcgat aactatctcg
     5461 gacttggtcg tagcagttta tccgggcagt cgatgtcgga aatcttcctc aatatcaaag
     5521 agaaaacatt ggcgtggaag ccggatatca tccgcctgtg gaaattccct aaagagatga
     5581 aagagttcac catcgaccag cagaaaaaca tgattgcctt ctcgggtagc catttccgtt
     5641 tgccgctgct gttgcgggtt tcagacaaac gcgtggaacc gctgccggaa agcgaatact
     5701 cagcaccgct gcgtttccag ctggccgatt tcgctccacg cgacaatttc gtctgggttg
     5761 accgttgcta taagatggca caactctggg ctccggaact ggcactctcc accgactggt
     5821 gtgtctcgca agggcagctt ggcggtcagc aaattgttca gcatgttgac aaaacaacat
     5881 ggcagggcaa aacggcattt aaagatacgg tcatcgacat ggcgcgttat aaaggcaatg
     5941 tcgatacgct gaaaattgtt gataacgata ttcgctacaa agccgacagt ttcatcttca
     6001 acgtcgccgg tgcgccagaa gaggtgaaac agtttagtgg gatttcccgt ccagagtcgt
     6061 ggggccgctg gtccaacgcg cagctgggcg atgaagtaaa aatcgagtac aagcatccgc
     6121 tgccaaagaa atttgacctg gtgattaccg ccaaagcata cggcaataac gccagccgtc
     6181 ctattccggt acgcgtaggc aatgaagaac aaacccttgt gctgggcaat gaagtgacca
     6241 ccaccacact gcatttcgat aacccaaccg atgccgacac actggtaatt gtgccgccgg
     6301 aacctgtctc aaccaacgaa gggaatatcc tcggacactc gccgcgtaag ctcgggatcg
     6361 gcatggtgga aattaaagtg gtggaacgtg aaggataatt cctgactgat gtgtaggtcg
     6421 cataaggtgc gacctacaca ccttctgcgg cctacaaaac atcattgaga aacagaaaaa
     6481 aacagcgccc aacacttccc cggacgctgc atggcaactt taataatctc gccttatcag
     6541 aaagtaatta cacccttaat caactcacga ttgttaatca catcgcgctc gtaggtttct
     6601 gccagcgtgg cgaacggata gcgatgggtt aacatcatgt cagcagtgat ttttccttcc
     6661 gccatcagtc gaccgacttt agcaaaatct tccggcgtgg cgttgcggct acccatcatc
     6721 gtcgtttctt ttttatggaa ttccggatcg gagaactgca actcaccttt aaacaggccg
     6781 acaaatacca ccgtgccgcc gtgacgaatc aaattcacgg tgttattcat cgcatgttga
     6841 ttacctgtcg cgtcgatcac tttctgcgcc agcgaaccac caaactgcgc ccgtagctgc
     6901 gcgtcaaaat cttcggctga cgggtccagt aaaggtaatt ccagacgcgt tgccacatgt
     6961 tcacggcgcg ccggactggt atccgccacc accacctgtg cgccatcggc tttagcgatt
     7021 gccgccgcgc ccagaccgat tggccccgcc ccgaccacca gcacctgctc gccgggagca
     7081 atggctgcgc gacgcaccgc atgagcgcta atagcgaaag gttcaatcaa tgctgccgcc
     7141 tgcgggtcaa taccgtctgc gggcaaaatg ttcgccaccg gcacgctcaa atactcacta
     7201 aaaccgccat cctgatgcac gccaatgact gaaatttttt cacagcaatt ggtacgcccg
     7261 cttttacacg ccgggcattg ctgacaggca acataaggga tcacagcaac ttgctgacca
     7321 tttttaagat cagcaatatt tttacccagc ccaacaatct ccccacatat ttcatggcct
     7381 aaaacacgtg gataactaaa aaatggttga tttccacccc aggcatgaat atcggtaccg
     7441 caaatcccga cagacttaat ttttattaat gcttcattgt cacccggaat aggtatctcg
     7501 cgttgtttcc agactaattc tttcggctgc tggcaaatta aaacattcat cgtagacatg
     7561 attcggctct ccattttttg ttgccttaga tatcaatgaa aatgaaaaat ggcagcggaa
     7621 cggaaaatct gttttgtgaa aacacacgca taaaaatgtt ttaaatcggg ttttaatgga
     7681 ctcacaagga ggaaaatatg agtcgttcac aaaatttacg ccacaatgtg attaaccagg
     7741 tcattgatga tatggcgcgg ggtcatatcc cttccccact tccatcgcaa agtgcgctgg
     7801 cggaaatgta caacatcagc cgcaccacag tgcgtcatat tctcagccac ttacgcgaat
     7861 gcggcgtctt gacgcaggtt ggaaacgact atgttattgc gcgtaaacct gaccatgatg
     7921 acggtttcgc ctgtactacc gcttcaatga gtgaacaaaa caaagtgttt gagcaggctt
     7981 ttttcacgat gataaaccag cgccagttac gcccagggga aacgttttct gaactgcaac
     8041 tggctcgggc cgcgggtgtc agcccggttg tagtaagaga atatctttta aaattcggcc
     8101 gttataatct tattcacagc gaaaagcgcg gccagtggag catgaagcaa ttcgatcaat
     8161 cctacgccga gcagctgttt gagctacgcg aaatgctcga aacacattca cttcagcact
     8221 ttctcaacct gcctgatcat gatccccgct ggctgcaggc gaaaaccatg ctcgaacgtc
     8281 atcggctact gcgtgacaac atcggcaata gctttcgcat gttctcgcaa cttgacagag
     8341 attttcactc cttactgctt tcagctgccg acaatatctt ttttgatcaa tcgcttgaga
     8401 tcatctcagt catctttcat tttcactatc aatgggacga aagcgatctt aaacagcgca
     8461 atatcattgc cgtcgatgag catatgacca ttctcagcgc tctgatttgc cgcagtgacc
     8521 ttgatgccac ccttgcgctc cgtaaccatt tgaattcagc taaacaatca atgattcgat
     8581 ccattaatga gaacacgcgt tatgctcatt aagtacggat taaaaacagc aaactattag
     8641 cgggtgcacg gttttatcaa aagataaata ccgggaaatt cccgcttacc tatgctcaca
     8701 atcagtcgac taaaaatgtt tttgtgagcg gtagtaaagt cctaaaactt taacctgccg
     8761 ttaatgtcaa ccttgcgtaa ttcacaatta cgtaaacaat taaaattata aatacaggag
     8821 tccggcgtgg aaaaagaaaa tatcaccctc gatccgcgtt cttcatttac tccatcttcg
     8881 tcggcagata ttcccgtgcc accagatgga ttagttcaac gcagtacccg aattaaacgc
     8941 attcaaacca ccgccatgtt gttattattt tttgcggcgg taatcaatta tctcgaccgc
     9001 agttcgctgt cggtagcaaa tttaacgatt cgtgaagaat tgggattaag tgccaccgaa
     9061 atcggcgctt tgctctccgt gttttcactc gcttacggga ttgcgcaact tccttgcggc
     9121 ccactattgg atcgtaaagg cccacgcctg atgctgggac tggggatgtt cttctggtca
     9181 ctgttccagg caatgtctgg catggtgcac aactttacgc agttcgtgtt ggtgcgtatc
     9241 ggtatgggga ttggtgaagc gccgatgaac ccatgcggtg taaaagtcat taacgactgg
     9301 ttcaacatca aagagcgcgg acgcccgatg ggcttcttca acgcagcttc taccattggc
     9361 gttgccgtaa gcccaccgat tctggcggcg atgatgctgg tgatgggctg gcgcgggatg
     9421 tttattacca ttggtgtact ggggattttt ctcgccatcg gctggtatat gctctatcgc
     9481 aaccgcgagc acgtagaact gactgccgtt gaacaagctt atctcaatgc aggtagcgtc
     9541 aatgcccgcc gagatccgct cagttttgcc gaatggcgca gcctgttccg taaccgtaca
     9601 atgtggggaa tgatgctcgg attcagtggc atcaactaca ctgcgtggct gtatctggcc
     9661 tggcttcctg gttacctgca aacagcctat aacctggatt taaaaagcac agggttgatg
     9721 gcggctatcc ctttcctgtt tggggctgcc gggatgctgg tcaacggtta cgttactgac
     9781 tggctggtca aagggggaat ggctccgatt aaaagccgta agatctgcat tattgccggg
     9841 atgttctgtt ctgccgcctt tacgctggtc gtaccgcaag cgacaacatc catgacagcg
     9901 gttctgctga ttggcatggc actgttctgt attcactttg ccggaacatc ctgctggggc
     9961 ttgatccacg tcgcagttgc ttctcgcatg actgcgtcgg tgggcagtat ccagaacttt
    10021 gccagcttca tctgcgcctc ttttgcgccg atcattactg gttttattgt tgataccacc
    10081 cactcattcc gtctggcact aatcatctgc ggttgcgtca ccgcagcggg ggcactggcg
    10141 tacatcttcc tggttcgtca gccgatcaac gacccacgta aagattaatc tccttatgcc
    10201 cgataacatt ttgcttatcg ggcattttca tggcgattaa aatgtttccc agttctcctc
    10261 gctatctgcc acggccattt tacgcggcgc agctggcgtc acggttttta ccacagccga
    10321 tgtttcacgc tgctgttgct gaatccggaa cactgccact gcttcggtca ggcgactggc
    10381 ctgctcttcc agcgcggcgg cggcagcggc agactcttcc accagcgcgg cgttctgttg
    10441 agttacccgg tccatctcag caaccgctaa gccaacctga tcgataccac ggctctgctc
    10501 atcagaagca gaagcaattt cgcccataat gtccgtcacg cgggtcacgg cgctgacaat
    10561 ctccgccatt gtttccccgg cgctttcgac cagcgtagag ccaacatcca ctttccccac
    10621 cgagtcttca atcaggcttt taatttcacg agccgcctgg gcgctgcgct gggccagatt
    10681 acgcacttct cccgcgacca ccgcaaaacc gcgcccttgc tcacccgcac gcgcagcctc
    10741 aaccgccgcg ttcaaagcca gaatattggt ctggaaggca atgccgtcaa ttacgctgat
    10801 aatatcggcg attttctgcg aactggtgga gatatcgcgc atagtctgca ccacgttatc
    10861 taccactttg ccgccgcgtt gcgccgtttc agaagcactt aacgccagat ggctggcctg
    10921 gcgcgcattt tcggcgttct gcttcaccgt tgcggtcagt tactccatgc tggctgccgt
    10981 ctcttccagc gaagcggcct gttgctcggt gcgcgaagag agatcgttat tgccggtggc
    11041 gatttcgctg gcaccgctat agatggcatt ggccccgttg cgcacatcac cgacggtacg
    11101 catcagctct ccctgcatat ggcgcaaact ctctgccagt tgccccatct cattagagcc
    11161 atccacctca atcggtttca ccagatcgcc gcctgcaata tgacgaatgc tgtcaatcag
    11221 gcgattcatt ggcgctacca gcgaggcttt aataccgaac cagacggcga agatgaccgc
    11281 cagtacgacg atcatcacgc ccaccagaat ccacatcgcc tggctgtagg aggcattgtt
    11341 atcgctgacg gcgatatcat agagccgatc gttttgctcc atgtaagcca catactgctt
    11401 ctcgaaaccg tcctgatatc cctgggtcgg ctgatcaaag aactcgttga ttttgcctgc
    11461 acctaacagt tggatcagct ccgccagcgc attgtgataa atatcgtaat tacgtttgat
    11521 ctctgccgct gcggcggtgc tctgacgcgg gtcacgcggc aacgcttcgt aatccgccca
    11581 gtttttttcc gcctgtttca gcgaaatact ggcactctgc atcagctcag caacggttga
    11641 accgctacca atattgttct gatccatcat gtagcggata cccgcgcggt tgagggtgtt
    11701 acgcgtctgc aacaacgcga cccagctgcc attcagcgtg gattgctgct ggcgaatggt
    11761 ttgtaaaaca gtgaaatttt ctttgtcatt ctttaaggca ttaaagaaca gaccgcctga
    11821 tgtcagttgt aaaaggccaa aaacggccaa aaccagcagt aagctggtca cgatcttgat
    11881 acgttttaac atggtttctc tttcctgtgg accgatgcaa gattttcggc ctggaaagca
    11941 aaaactttat gaaaaggtgt aacccaccat ccatggaaaa accaggcgct ggttatcaga
    12001 tactaaaaag ttattcatta tcgcccggga tggcttcttg tcgggaattg cccagagcaa
    12061 taaattcctc taacagatgg gttaactgtt gcattttctc ggcagtaaac tgagcttcaa
    12121 tctgccgata ggcttcttca atctgtgtct gagcgcggtt atacagcgcc tgcccctctt
    12181 tggttagcga gatatagagc ttccgctgat cattgatcgg ctttaatcgc aacactaaac
    12241 cgtcgcgttc catccgcgtg aggatcccgg tcaggcttgg gcgcaaaatg caggcgcgat
    12301 acgccagatc gtgaaaatcc attgatgggc tttccgccag aatacgcacg atgcgccact
    12361 gttgctcagt caaattatgc cgtttaacga ttgggcgaaa ataactcatc gccgcttctc
    12421 gcgcctggag caacgcaatg gttagtgagt cgtgcattat ctttcccctg aaaaattcaa
    12481 aatcattaat atagaaacag ttaatcgtca cgttaccggg actgcgctgc ccctgcaatt
    12541 cctgatttta tcaaaattga tgatatttta ttaacttact gatatttaat attttattat
    12601 ctttaaatgt aaatagtttg ttaattagat cacatttaca tcacttactt ttgccaaact
    12661 acgcaatcgg gaataaaagc aatcattaac atattaatga ttaagatgat cgaatccgag
    12721 gagtggtaat gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc caacttgatg
    12781 catggcagga agcgttccag caatccccct acaaagcccc gcctaaaact gcggtctggt
    12841 ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc gattcccttt ccacagggtg
    12901 aaaaggtact gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg acgaaagtac
    12961 gtgaagaaga tgcggcagag tacatcgccg gatatgcgct ggctaacgac gtcagcctgc
    13021 cggaagagag cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga ttctgcccca
    13081 ttggcgaaac cgtggctctc agcaatgtcg ataatctgac catctatacc gagatcaacg
    13141 ggcgtcctgc cgatcactgg aataccgccg atttacaacg taacgccgcg cagttgctga
    13201 gcgccctgag cgaatttgcc acgctgaatc caggcgacgc cattctgctc ggcacgccac
    13261 aggcgcgcgt ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa ggtttcccgc
    13321 cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac gcacaagagc ttcccaacgc
    13381 agccacaccc gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac cacgccagcg
    13441 aactggaatt taagccaccg gaagaaccgc tggtgttcct gaaagcgcca aataccctca
    13501 ctggcgataa ccagacctcc gtgcgtccaa acaatattga atacatgcac tatgaagcgg
    13561 agctggtggt agttattggc aaacaggcgc gtaacgtcag cgaagccgat gccatggatt
    13621 atgtcgcggg ctacaccgtg tgtaacgact acgccattcg tgactatctg gaaaactact
    13681 accgccctaa cctgcgggta aaaagccgcg acggactgac gccgatgctt tcaaccatcg
    13741 tgccgaaaga ggcgatcccg gacccgcata atctgaccct tcgcaccttc gtcaacggcg
    13801 agttacgcca gcaaggcacc accgccgatc tgatcttcag cgtgcccttc ctgatcgcct
    13861 acttaagtga atttatgacc ctgaatccgg gcgacatgat cgccaccggc acacccaaag
    13921 gcttatctga cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc gtgggccgcc
    13981 tggtgaaccg aattgtgagt gaggatacag cgaaatgaaa aaagtaaatc attggatcaa
    14041 cggcaaaaat gttgcaggta acgactactt ccagaccacc aatccggcaa cgggtgaagt
    14101 gctggcggat gtggcctctg gcggtgaagc ggagatcaat caggcggtag cggcagcgaa
    14161 agaggcgttc ccgaaatggg ccaatctgcc gatgaaagag cgtgcgcgcc tgatgcgccg
    14221 tctgggcgat ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa ccgcggacac
    14281 cggcctgccg atccatcaga ccaaaaatgt gttgatccca cgcgcttccc acaactttga
    14341 atttttcgcg gaagtctgcc agcagatgaa cggcaagacc tatccggttg acgacaagat
    14401 gctcaactac acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc cgtggaacgt
    14461 accgtttatg accgccacat ggaaggtcgc gccgtgtctg gcgctgggca ataccgcggt
    14521 actgaaaatg tcggaactct ccccgctgac cgctgacagc ctgggtgagc tggcgctgga
    14581 agccgatatt ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa ccgcagggga
    14641 tgcgctggtt cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta cggccaccgg
    14701 gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggaactgg gcggtaaatc
    14761 gccggtgctg atttttgaag atgccgatat tgaacgcgcg ctggacgccg ccctgttcac
    14821 catcttctcg atcaacggcg aacgctgcac cgccggttcg cgcatcttta ttcagcaaag
    14881 catctacccg gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc gcgtgggcga
    14941 tccgaacgat ccgaataccc aggttggcgc gcttattagc cagcagcact gggaaaaagt
    15001 ctccggctat atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg gcggcccgga
    15061 taaaccgtcc gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc caaccgtgct
    15121 ggcagacgtt gataaccgta tgcgagtcgc ccaggaagag attttcgggc cggtcgcctg
    15181 cctgctgccg tttaaagacg aagccgaagg cttacgtctg gcaaacgacg tggagtacgg
    15241 cctcgcgtcg tacatctgga cacaggatgt cagcaaagtg ttacgcctgg cgcgtggcat
    15301 tgaagctggc atggtgttcg tcaacaccca gaacgtgcgt gacctgcgcc agccatttgg
    15361 cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg aagtgttcgc
    15421 ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat ggggagtctg
    15481 atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcgatg tatcactctg
    15541 aactgccagg gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat aaagagatca
    15601 gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc cactggctgg
    15661 tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac accagcaacg
    15721 agctgccgca ttttatccgc gatatgacct acaactacga gggcaacccg gagttggggc
    15781 agcttattgc cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac aacattccca
    15841 gcctgaaact ggaatacggc acgctggtgc cgatgcgcta catgaatgaa gacaagcact
    15901 tcaaagtggt ctccatttcg gctttctgca cggttcacga ttttgccgac agccgcaagc
    15961 tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg gtccttgcca
    16021 gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc ggaagaaggg atgaacagct
    16081 acacccgcga gttcgaccgc cagatggacg agcgcgtggt gaagttgtgg cgcgaaggcc
    16141 agttcaaaga gttctgcaat atgctgccgg agtacgccga ctactgctac ggcgaaggca
    16201 atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac gacggcaagg
    16261 tggagtttat caccgagcta ttcccaagct ctggcaccgg tcaggttaac gctgttttcc
    16321 cgcttcccgc gtaaggacgt tttatgccgc actttatcgt taaatgcagt gacaacatcc
    16381 gcgaagaagc cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg gcagccacgg
    16441 gtatttttcc gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc tggcagatgg
    16501 ccgacgggca gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc gcaggtcgca
    16561 gcctggaaag ccgccagcag gcgggtgaaa tgctgtttga actgattaaa acgcacttcg
    16621 ccgccctgat ggagagccgc ctgctggcgt tgtcgtttga gattgaagag ctgcatccga
    16681 cgctgaattt taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca gattgcccgg
    16741 tggcgctgcg cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc cggataaggc
    16801 gcagccgcat ccggcaatgc cacaggatat cgctatgttc gataaacaca cccacaccct
    16861 gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg cgatctcgct
    16921 ggattacccg gagatcacca tcgaagacgc ttacgcggtg cagcgtgaat gggttcgcct
    16981 gaaaatcgcc gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct cgaaagcgat
    17041 gcaggcaagc tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg acatgttctt
    17101 ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg aagtggagct
    17161 ggctttcgtg ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg acgtctacaa
    17221 cgccacggac tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc acaacatcga
    17281 tccggaaacc cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg ccgccaacgc
    17341 cggggtgatc ctcggcggtc gtccgatcaa accagacgaa ctggatctgc gttggatctc
    17401 cgccctgatg tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg gcgtgctgaa
    17461 tcatccggca aacggcgtgg cctggctggc gaacaaactc gcaccgtatg acgtacaact
    17521 ggaagccggg caaatcattc tcggtggttc gttcacccgc ccggttccgg cgcgtaaggg
    17581 cgacaccttc cacgtcgatt acggcaacat gggttccatt agctgccgct ttgtttaagg
    17641 agagaacgat ggaaaacagc tttaaagcgg cgctgaaagc aggccgtccg cagatcggat
    17701 tatggctggg gctgagtagc agctacagcg cagagttact ggccggagca ggattcgact
    17761 ggttgttgat cgacggtgag cacacgccga ataacgtgca aaccgtgctc acccagctac
    17821 aggcgattgc gccctacccc agccagccgg tagtacgtcc gtcgtggaac gatccggtgc
    17881 aaatcaaaca actgctggac gtcggcacac aaaccttgct ggtgccgatg gtacaaaacg
    17941 ctgacgaagc ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt attcgcggtg
    18001 tgggcagtgc gctggcccgc gcctcgcgct ggaatcgtat tcctgattac ctgcaaaaag
    18061 ccaacgatca aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg aagaacttac
    18121 cgcagattct ggacgtggaa ggcgtcgacg gcgtgtttat cggcccggcg gatctgagcg
    18181 ccgatatggg ttatgccggt aatccgcagc acccggaagt acaggccgcc attgagcagg
    18241 cgatcgtgca gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc aatgagcaac
    18301 tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt gacaccaccc
    18361 tgctcgcccg cgccgccgaa gcgctggcag cacggtttgg tgcgcaggcc accgccgtga
    18421 agcccggcgt gtattaatgc cggatgcgct gcgcttatcc ggcctacact cgcaccaaac
    18481 cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa atcgcggacc
    18541 ggataaggcg ttacgccgca tccgccataa accttgtcgt accctacaaa aatcccatta
    18601 gaggaagaaa aatgagcgac acctcacctg ccataccgga gagtatcgat ccggcgaatc
    18661 agcataaagc gctgactgcc ggacaacagg cggttattaa gaagctattt cgccgcctga
    18721 gcgtctttct gttcgtgctg tttatcttct cgttccttga tcgcatcaac atcggctttg
    18781 ccggactcac gatgggacgc gacctcggtc tgagcgccac catgtttggc ctcgctacca
    18841 ccctgttcta tgccgcttat gtcatcttcg gcattcccag caacattatg ctgagtattg
    18901 tcggtgcgcg gcgctggatc gccaccatca tggtgctctg gggcatcgcc tctactgcca
    18961 ccatgtttgc cactggcccc accagcttgt acgtactgcg tatactggtt ggcattaccg
    19021 aagccggctt tctgcctggt attctgctgt atttaacctt ctggtttccg gcctatttcc
    19081 gcgcccgtgc caacgccttg tttatggtag caatgccggt aacgacagcg ttgggctcga
    19141 tcgtttccgg ctacattttg tcgctggatg gcgtcatggc attaaaaggc tggcagtggc
    19201 tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg ttctggcttg
    19261 atgactcacc ggacaaagct aagtggctga cgaaagaaga caaaaaatgc ctgcaagaga
    19321 tgatggataa cgatcgtctg acgctggttc agccagaggg agccatcagc caccacgcca
    19381 tgcaacaacg cagtatgtgg cgggagatct tcactccggt ggtgatgatg tataccctgg
    19441 cgtatttctg cctgaccaac acacttagtg cgatcagcat ctggacaccg cagatcctgc
    19501 aaagttttaa tcagggcagc agtaatatca ccatcggcat gctggccgcc gtaccgcaga
    19561 tttgtaccat tctcgggatg gtctactgga gccgtcactc agatcgccgc caggaacgaa
    19621 ggcatcacac cgcccttcct tatttgttcg ctgccgcagg ttggttactg gcttcggcaa
    19681 ctgatcacaa catgatccag atgctgggga tcattatggc ttcgaccgga tcattcagcg
    19741 caatggcgat tttctggaca acaccggatc agtccatcag cctgcgggca cgagcgatcg
    19801 gtattgcggt gatcaacgcc actggcaaca ttggttcagc gttaagtccg tttatgatcg
    19861 gctggttgaa agatttgacc ggcagcttta acagtggatt gtggtttgtt gccgcgctgc
    19921 tggtgattgg tgcggggatt atctgggcga ttccaatgca gtcctcccgt ccgcgagcga
    19981 ccctgtaagg aacgacgatg tgtgaccgtc agattgccaa tattgatatc agcaaagagt
    20041 acgatgaaag cctgggcacg gacgatgtgc attatcagtc gttcgcccgc atggcggcat
    20101 tttttggccg ccatatgctg ccacatcgcc acgaacagta ctttcagatg catttcctca
    20161 atagcggaca gattgagcta cagcttgacg atcatcgcta ctcggtggaa gcgcccctgt
    20221 ttgtcctgac gccgccgtca
//
LOCUS       DAZPEP010000066        20216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZPEP010000066 DAZPEP010000000
VERSION     DAZPEP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.066"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..696
                     /locus_tag="ACHUT9_002331"
                     /pseudo
     CDS             1..696
                     /locus_tag="ACHUT9_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(761..1472)
                     /locus_tag="ACHUT9_002332"
                     /pseudo
     CDS             complement(761..1472)
                     /locus_tag="ACHUT9_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
     gene            complement(1472..2713)
                     /gene="clsB"
                     /locus_tag="ACHUT9_002333"
     CDS             complement(1472..2713)
                     /gene="clsB"
                     /locus_tag="ACHUT9_002333"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFR1348789.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2710..3471)
                     /locus_tag="ACHUT9_002334"
     CDS             complement(2710..3471)
                     /locus_tag="ACHUT9_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1348790.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3604..4014
                     /locus_tag="ACHUT9_002335"
     CDS             3604..4014
                     /locus_tag="ACHUT9_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFR1348791.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(3976..5082)
                     /locus_tag="ACHUT9_002336"
     CDS             complement(3976..5082)
                     /locus_tag="ACHUT9_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1348792.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5093..6235)
                     /locus_tag="ACHUT9_002337"
     CDS             complement(5093..6235)
                     /locus_tag="ACHUT9_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1348793.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQIWQMQRAEDN
                     GQTFEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEA
                     LLSTEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLL
                     STLGMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPAR
                     YFVSTLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6228..7964)
                     /locus_tag="ACHUT9_002338"
     CDS             complement(6228..7964)
                     /locus_tag="ACHUT9_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1348794.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(7957..8952)
                     /gene="hlyD"
                     /locus_tag="ACHUT9_002339"
     CDS             complement(7957..8952)
                     /gene="hlyD"
                     /locus_tag="ACHUT9_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFR1348795.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(8955..9626)
                     /gene="cecR"
                     /locus_tag="ACHUT9_002340"
     CDS             complement(8955..9626)
                     /gene="cecR"
                     /locus_tag="ACHUT9_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFR1348796.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9855..11219
                     /gene="rhlE"
                     /locus_tag="ACHUT9_002341"
     CDS             9855..11219
                     /gene="rhlE"
                     /locus_tag="ACHUT9_002341"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFR1348797.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11451..11933)
                     /gene="ybiA"
                     /locus_tag="ACHUT9_002342"
     CDS             complement(11451..11933)
                     /gene="ybiA"
                     /locus_tag="ACHUT9_002342"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFR1348798.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12053..14203
                     /gene="dinG"
                     /locus_tag="ACHUT9_002343"
     CDS             12053..14203
                     /gene="dinG"
                     /locus_tag="ACHUT9_002343"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFR1348799.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14231..15193
                     /gene="ybiB"
                     /locus_tag="ACHUT9_002344"
     CDS             14231..15193
                     /gene="ybiB"
                     /locus_tag="ACHUT9_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFR1348800.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVAMGEAATISDGLARVNQAF"
     gene            15334..16419
                     /gene="hcxB"
                     /locus_tag="ACHUT9_002345"
     CDS             15334..16419
                     /gene="hcxB"
                     /locus_tag="ACHUT9_002345"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFR1348801.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            16486..16545
                     /gene="ybiE"
                     /locus_tag="ACHUT9_002346"
     CDS             16486..16545
                     /gene="ybiE"
                     /locus_tag="ACHUT9_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFR1348802.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16648..16908)
                     /gene="ybiJ"
                     /locus_tag="ACHUT9_002347"
     CDS             complement(16648..16908)
                     /gene="ybiJ"
                     /locus_tag="ACHUT9_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFR1348803.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17173..17439)
                     /gene="ybiI"
                     /locus_tag="ACHUT9_002348"
     CDS             complement(17173..17439)
                     /gene="ybiI"
                     /locus_tag="ACHUT9_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFR1348804.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17513..18190)
                     /gene="ybiX"
                     /locus_tag="ACHUT9_002349"
     CDS             complement(17513..18190)
                     /gene="ybiX"
                     /locus_tag="ACHUT9_002349"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFR1348805.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18232..20216)
                     /locus_tag="ACHUT9_002350"
                     /pseudo
     CDS             complement(18232..20216)
                     /locus_tag="ACHUT9_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         4668 a         5306 c         5484 g         4758 t
ORIGIN      
        1 aacagcaata aattagattt tcagcacgag gccaggatat aaaacggtat atggctcatt
       61 accgctttag gtttggtggc aaccgcagga ctagcctggg gagctaagta tctcgaaatt
      121 acggcaacca aatatgattc accaccaatg tatgtcgcta tagggttatt attactttgt
      181 atgtatggct taagtaagga tatcaacaag ataaatgccg ccatcgcggg cgtaatatat
      241 ctgtttttac tctctttggt ggcgattgtc gttgcaagtt tagttcctgt atatgccatt
      301 atcatcgtgt tcagcactgc gggcgcgatg tttttaatca gtatgctggc cggtttatta
      361 tttaatgttg agcctggttc tcaccgtttt atcattatga tgacgttgac agggttggcc
      421 ctggtaatca tcgtgaatgc ggcattaatg agtgaacggc ccatttgggt aataagttgc
      481 ttaatgattg tgttatggcc aggcattatc tcgcatggac gaaataagct ccttgaactg
      541 gcaggaaaat gccatagtga agagttgtgg agtccggttc gttgcgcttt tacaggtgca
      601 ttaacactct attactattt tatcggcttc tttgggatac ttgccgcgat agctataacg
      661 cttgtctggc aaaggcatac gtgttttttt cattagccgt aaatgcacat actaaagaac
      721 ttaactaaac ttcacatcgc cgcttcattt ttcgcccgta gcccagtaca aagaaatagt
      781 tcacgctttg accaagtaac agccagataa tcgcccccat taccatccag ttaacgctgg
      841 aaatcagcat ctgagcgagg gcgaatttcc atgaaggcag caccagcttt tgccctttga
      901 tggtcatatg gcggtgcttc gcgaaagcgc aaaaccacaa ataaaccgcg ataatcatca
      961 gtaagccaat gccgagaata cgcagcgtag tttgatcgac ataccagtga tccggcaact
     1021 ccaccacgcc tgcggtaaag ataatccctg ccagtaaaat atagcccagc cagttggtgg
     1081 taatactgag cgagaaaatc cgcgtaatag tgctaccggg caaccccaga cgagagtaca
     1141 aacgatagcg cataccaatg ccgccgaccc aggtactgag cgtcaggttg aaggcgtagc
     1201 agataaacga caccagcatc acctggcgct tcgccagttt gtgaccgcag taaaagcggg
     1261 cgagcaggtc atagcagccg taaatcagat agctgacgac caccagcgac cgcactaagc
     1321 agcgcaacgc gattgtagtc gcggatgacc ttccagacct cttcccagtc cacttttttg
     1381 gcgtagacca ccagcaacac aatcaccgcg ataaaaaaca gccaggtgag gatcttcttt
     1441 gctaagcgcc agcgcgggtg tgatttactc atcagggttt tacccccgtg ttttcagttt
     1501 ctacccgatc ctgcgtttcc attgtcggtt gtgcgggcgg atcaacctgc gccagacgtg
     1561 gcgtgtgtgc cggaagccag ccaaccagcg ccgggaagtg gcgtaaaaag tggaacgcca
     1621 gcacgctttt ggtcaggttc caccaggtgc gtttgggcag catggtttca tccacctgct
     1681 gacaatctgc ggcgataatg ccgttcagat tatcgcgcag cgtctggtta aaattacgat
     1741 cgtggatgat gacatttgct tcgagattca gtgacaaact gagcggatcg agattactgg
     1801 accctactgt cgcccagtga tcgtccatca atgctacttt gccgtggagc gggcggcggc
     1861 ggtactcaaa cacctgaacg ccgcctttaa ccagatagtt atacagcaag cgcgcgccga
     1921 ctctgacaat cggcatatcc ggttcgccct gaatgatcag tttgatccgc accccgcggc
     1981 gtgccgcttt acgcaaggcg tgtaaaaatc gatagccggg gaagaagtag gcgttggcga
     2041 taatgacttc ccgccgcgcc tgagtgagca ttttcagata gtggcgttct atatcatcgc
     2101 ggtgttcttc gttatcgcgc cagaccagca atacctgcgc gtctcccggc tggcggttct
     2161 cttccgcttt gtgatgacgt cgccaccagc gtcgtgccgc gctctgtcca ggcaggtttt
     2221 ccagctcaaa ctggagaata tcttcgacaa tcggcccttc aaggcgtacc gcgtaatcct
     2281 gtttagcctc tggaccgtag ctggacatat gctcggcgga gtaattcagc ccgccaataa
     2341 aggcgatacg cgcgtcgatc accacaattt tgcgatgcat ccggcgaaac acattggtgc
     2401 gcataccaaa aaggcgaggg cggggatcgt agtagcggaa cactacgcca gctgccgtca
     2461 gttcattgac aaactcatcg ctgagatccg gcgaaccgta gccatccagc aagacttccg
     2521 ctttaacccc gcgttgcgct gctgccagta gtgccgcatg cagctgtttg ccgacgttat
     2581 cctcaaacca gataaacgtt tcaagaatga tgcgttcttg tgcctcgcca atcgccttaa
     2641 acaccgcggg ataatattgc tcgccgtttt ccagcaactg gatcttattg ccttcgcgcc
     2701 agctacattt cataaatgaa tctccgcact taaaggggca tgatcagaaa ggtgtcgcca
     2761 tgtccgcagc ggcaacgcgg ttggcgcgct ggcgctggca tttttgacgt agatcctgtc
     2821 cagtcgtagt agaggaaatt gcaccggaaa cgtgcgcgcc gggcgtccgt gggcgcgggt
     2881 aaaaatctca tccagtccgg cctgcacttt taacggatga ttagcttttt gccgccagtc
     2941 attgaaatca cccgccacca ataccggttc gccgtccggt agctcattca cccattcggc
     3001 gagcatcgca agctgcgcct gacggtgcgc ctcacgcagg cccagatgta cgcacatcac
     3061 atgaatcgct tttccggtca tcggcggcac aatgcggcag tagagcacgc cgcgcttttc
     3121 cgcaccatcg accgaaacat cgcgattctc ataatgttca atgggataac gcgacagtac
     3181 ggcgttgccg tgatgccctt ccgggtatac ggcattgcga ccgtaggcaa aatcgctcca
     3241 catagtgtcg gcgagaaact cgtagtgcga ggtatcgggc cagttttcca catgcagcgg
     3301 atgaacttcg tgcgcgccca tcacttcctg caggcaaaca atatcggcgc tgacggtacg
     3361 cacggcatcg cgaagttccg gcaaaatgaa gcgtcggtta aacgcggtaa agcctttgtg
     3421 aatgttgatg gtgagcacct tgaacgaaaa ttgttgtgtt tgatcgggca taattttcct
     3481 gtctttgcct cttatctcat tgaaatagtg tagtcggcgt cacaaaaagg tgcggtctta
     3541 cggaattttc cgtaaagttc ggtactctga gtaagtagag ataaattctt caggagagaa
     3601 gccatgaagt ggcaacaacg tgttcgtgtc gcaacgggtc taagttgctg gcagattatg
     3661 ttgcatttac tggtagtggc gctgctggtg gtgggctgga tgagtaagac tctggttcac
     3721 gtcggcgtgg gattatgcgc actgtattgt gtcacggtag tgatgatgct ggtgtttcag
     3781 cgccaccccg agcaacgctg gcgtgaggtg gcagacgtgc tggaagagct gaccacgacc
     3841 tggtattttg gcgcagcgct gattgtgctg tggctgttgt cccgcgtact ggaaaacaac
     3901 tttttgctgg caattgcagg gctggcgatc cttgctggcc cggcggtagt gtctttgctg
     3961 gcgaaagata agaagttaca tcaccttacg tctaaacatc gcgtacgccg ctgaccctgt
     4021 cgtggccgtt atcaccagta gcggccacaa actattccac acaatatcca gactcgcatc
     4081 cttcaaataa atctgcttgg taatgtccgt aaagtggcga atagggttaa tccacgtcag
     4141 gttttgcagc catactggca tgttttccac cggagaaacg taaccggaaa ggagaatggc
     4201 gggcatcata aaaacaaaca cgccgataaa cgcctgctgt tgtgtcgagc agagtgatga
     4261 aatcaacaga ccgaatccca ccagcgataa accgtaaatc accatcgtaa agtagaacag
     4321 cgccagcgat ccggcgaagg ggatttgata cgcccagata ccaatcgcca gcacaatggt
     4381 ggcctggaag gtggcgacaa ttaacgccgg tacggctttg ccgatgaaga tctgccaggt
     4441 ggtgagcggc gaaaccagta gctgatcgag cgtaccttgt tcacgttcgc gggcgacgga
     4501 aagtgaagtg acgatcatta cgccgatagt ggtgatcatg gcgatcagcg acggcaccac
     4561 aaaccatttg tagtcaagat tcgggttata ccagttgcgt accaccagct cgctgttgtt
     4621 aggtttcggt tttccttcca gcagctcctg ctgataattt ttgacgatct gttgcaggta
     4681 gttggcggca atttgcgcac tgttggagtt acgcccgtcg aggatcaact gcaacggcgc
     4741 ggtctggaag gtatccagtt tgcgcgagaa gtcagccggg aaacgcacca gtagtaacgc
     4801 cttttgtgtg tcgatggttg ggcggatctc ctgtgggctt ttcagcagca gcacatgggt
     4861 aaaggcgctg gcgcgggcaa aacgttgggt cagctccacc gaatgctcgc cgttatcttc
     4921 atcgtagatg gcgatggtgg cgttagtcac ttccagcgtg gcggcgaacg ggaacaggat
     4981 cacctgaata agcacaggta aaatcagaat cgcgcgggtt tgtggttcgc gcagcaacga
     5041 ctgcaactct ttgcggatta acgtccataa gcgatgaaac atgctcttct ccctaatcca
     5101 gccgacgttt ggttttcagc cacgtcaggc cgataaacat caccgccgaa gcgatcaaaa
     5161 acagcacgtt taccaccagc accactggaa tattcccggc gaggaacagg ctttgcaggg
     5221 tgctgacgaa ataacgagcg ggaataatgt acgtcaccgc gcggatcacc gcgggcatac
     5281 tgtcgatctg aaaaataaag ccggaaagca taatcgacgg caggaacgcg gcgttcaggg
     5341 cgacctgagc ggcattgaac tggttgcggg taatcgtgga aatcagcagc cccatcccca
     5401 gggtactgag taaaaacagg ctggagataa aaaacagaat aagcagcgaa ccgcgatacg
     5461 gcacgccgag aataaacact gacaccagca tacacagcag catcgccagc atcccgagaa
     5521 agtaataagg gatcagctta cacagcagca gttccgtgcg ggtaatctcc gtagagagca
     5581 gagcctccat ggtgccgcgt tcccattctc gcgccaccac cagcgaggtg agaatcgcgc
     5641 cgatgaccgt catgatgatg gtcactgcac cggggataat gaagtgctgg ctaatcgccg
     5701 ccgggttaaa ccagtagcgg gtttgtacat caataagcgg ttcaaaagtc tgcccgttgt
     5761 cctccgctcg ctgcatttgc cagatctgcc agatctgcca gatcccttcg acatacccct
     5821 gtacaaagtt agcggtattc ggctcactgc cgtcggtgat cacctgaatc ggtgcggtgg
     5881 cgttggcgcg ctccatctgt tccgcaaaat ccaccggaat aaccaccaga ccgcgaattt
     5941 tccccgcctg cattttggcg atcagttcct gacggttatc gctgatggtg gcgtcgatgt
     6001 agggcgaacc ggtcatggtg tgggtgaaat ccagcgccgc ttcgctacgc tgttccagca
     6061 agatcccgac ccgcagcttg ctggagtcga ggttaatgcc ataaccaaaa ataaacagca
     6121 gtagcagtgg gatcactacc gcaatcagcc agctactcgg atcgcgaacg atctgccgcg
     6181 tctctttgac gcacagcgcc cgcacgcgac gccaggaaag gatcgggtta ctcattgcta
     6241 tgctccttat cccagtcgtg gatcaactga ataaaggctt gctccatggt gggatcgggt
     6301 tgctcatcgt tagccgactg tgctttcaaa tcgtccggcg taccgctggc gattaatttc
     6361 ccacggtaca ccaggccgat gcggtcgcaa tattccgctt catccataaa gtgggtggtg
     6421 accatcaccg tgacgccttt ctctaccatg ctgttgatat gtagccaaaa ttcacggcgg
     6481 gtgaggggat caacgccgga cgtcggttcg tcgagaaaca gaatgtccgg ttcatgcatc
     6541 agcgaacagg ccagcgccag ccgctgttta aaacctaatg gcagttcatc ggtggcgtgg
     6601 gaggcgatac ttttcaggcc gaacgcctcg ctcatgcggg agattttttc gttctgcgcc
     6661 cgaccgcgta agccatacac accagagaaa aagcgtaaat tctgttcgac cgtcaggtta
     6721 ccgtagagcg aaaacttttg cgccatatag ccgagatgct ggcgcgcttt accagaactc
     6781 tctttcagat ccatccccag taccagcgcc tgaccagagg tcggcaccag caaaccgcac
     6841 atcatcttaa aggtggtcga tttgcccgcg ccgtttggcc ccagcaaacc aaaaatctcc
     6901 ccgcgtttaa cggcaaagtt gacgtgatcg gtggcggcaa aatcaccaaa tttcttggtc
     6961 agttctttcg cttcgatcac cgtctcgccg ggtgtgcctt ctaccgtatg taatattgcg
     7021 cccagcggcg attccgaggt tccggcaccg cccagcaaat caataaacgc atcttcaaaa
     7081 cgcggcgtag tttcgttgat gttgatttcc ggcatcccgt cggcatggcg aatatcgtct
     7141 ggtgtggtct cttttttgag gatcagacgt accgatttcc cctgaatcat gccgtcgctg
     7201 acctgcggca gtttcaatgc ccgttgcaac agtttgcggt tgccctcgtg tggacttgtc
     7261 atcagaaagc tgcgtccggc catggtttgt gtcagggctt ttggttcccc ctgatacagc
     7321 aactcgcctt cattcatcag taacacgtca cggcactgct cggcttcgtc gagatacgag
     7381 gtactccaga ggattaacat cccttcgcct gccagctcat gcaccatctg ccacagttcg
     7441 cgccgtgaga tagggtcaac gccgacgccg ggttcatcga gcagcaacac tttcggttcg
     7501 cccaccaggg tacaggccag gccgagtttt tgtttcatcc caccggagag cttgcccgcc
     7561 aggcgtccgg taaacggccc aagagacgta aactccagca ggcgagcaaa agtttgctta
     7621 cgtgcctcgc cggtgacgct gcgcaaatcc gcgtacagat tgaggttctc catcaccgtg
     7681 agatcttcat acagaccaaa tttctgcggc atatagccga gcacggcgtg cagcgcgccg
     7741 tcgtttttga ttggatcaaa gccaatcacc gtggcattgc cgctgtcggg tttcagtaat
     7801 cccgccagca tccgcattag cgtggtttta cctgcaccat ccggccccac cagccccgtc
     7861 acataaccgg cgtgaatggt acaatcgagc ggcgcgacgg cgggcttgtc catgcccgga
     7921 aagcgttttt ccaggccgtt cagcgtgata acggcatcat tcatgtcctg cctcgttacc
     7981 gaattgcacc gtcaccggca ttccctggcg taacgcatca tcggcgtcgg tcaccacaat
     8041 acgcaggcga tagacgaggt cggtacgcag atccggtgtt tcgacggttt tcggggtaaa
     8101 ttcagcagtc ggcgaaacga aaccaatctg cccgtgatac ggcttgtccg ggcgaccatc
     8161 ggtataaagc agcactttgc gccccggctg ggcctggtca agataccgtt catcaacata
     8221 agcgcgcacc cacaccggac gcgttagtga aacggtaaac accgtgccac cttcattgag
     8281 gaccgtgcct ggctccaccg cgcgcgttaa cagcgtgcca tcagacgggg ctatcaacgt
     8341 tgagtcctgt aaattcaact ccgcctgcgc cagttgcgcc tgcgcctgtt cgaggctggc
     8401 tttcgcctgg gcgatgtcct gttcacggtt accggaacgg tactgacgca atttatcctg
     8461 tgctgatttc agcgttgcct gcgcctggtc gcgcgaggag cgggcatttt ccaggtcatt
     8521 tgccgaaata gtgcggcttt tccacagtcc ttgctggcgg ttatagaagt tctgcgcata
     8581 gtcataggcg gcttgcgcct gtttcaccgc tgcggcggcc tgagcgattt cttcatcgcg
     8641 atacccggca agcatcagat catactgcgc ctgtgccacc gagacgcccg ctttcgcctg
     8701 catcagggca atctcatacg gcttgtgatc cagttcgccc agcacctggc ccgctttgat
     8761 agcatcacct tcgtccaccg ccagcgattc aacgcgcccc ccaacacgga aactaagatt
     8821 caccgtacga atatccacgt tgccatacag cgtcaggccg ttatcctggc ggctttgata
     8881 ccaccagtag cctccggcaa ccacggcggc aagtaccact accgccagtt cgatcacgac
     8941 aggttttttc atcactacaa actcctttgc gataatcctt gcaaaatcag gtcgatatga
     9001 caggtcaccg tctggttgat cagttcggtc ttttcttcat cgaacgcggt ccagccggta
     9061 cgtaacagaa ttgtttcttt gccaagacgg aacgccagaa tctcaccaat caacgcatgg
     9121 gtatgaagga tcatgcgggt gtcattggcg tcgctgccgg tccaggcggc aatcaggcgt
     9181 gtcaggtggc tgtgtagcgg actaatcacc tgttcgtgca ccaggtggta ggctgccgtg
     9241 ggagagagct gctcacggga gataaacttg ctgaggttga cggtgtcatc ctgggtgagc
     9301 agcttaatca tgttcctgca ggcgcgaagg agcagttcac ggatggcagc ccgatcaggc
     9361 tgtggttggg cgaacaagcg ttcggcttcc tcggcatgcg gacggaactg ctcgccaata
     9421 aaatcggcaa tccactgggc gcaggcgagg tacaaatctt ctttcgaacc gaagtagtag
     9481 gtgatggcag cgatattctg cccggcttgg gcggctatct cgcgggtggt ggcgttcatt
     9541 ccatattcac caaactgcgc cagtgcggca gcaatcagct gttttttcgc ctgttcaccc
     9601 ttgattgtca tggcaggatt attcatcgca cagtccattc ttaatcaaat gattgattaa
     9661 gattatgact ccatatggga gttgtccagt atggctaaga attttagcta cgccagtcac
     9721 agggataatt tatgcgctgc gtcacaaaaa ctgctacact ccgctcgctc atgacattgt
     9781 ggtttttgtc attttccttt tcagtatctc cctgaaaact acaccggtaa cggtcggggc
     9841 ggttcggagt agtcatgtct ttcgattatt tgggtttaag ccctgatatc ctgcgcgccg
     9901 ttgccgagca gggttaccgt gaacccaccc ctattcagca gcaggcgatc cctgcggtgc
     9961 tggaaggccg cgacctgatg gctagcgccc agaccggcac cggcaaaaca gcgggcttta
    10021 cgctgccgct gttgcaacac ctgatcactc gccagccgca cgccaaaggg cgtcgtccgg
    10081 tacgtgcgct cattcttacc ccgacccgtg aactggcggc gcagattggc gaaaacgtcc
    10141 gtgattacag caaatacctg aacattcgtt cgctggtggt gtttggtggt gtgagtatta
    10201 acccgcagat gatgaaactg cgtggcggcg ttgatgtgct ggtggcaacg ccgggacgtt
    10261 tgctggacct ggaacatcag aatgcagtga agctggatca ggttgaaatc ctcgtcctcg
    10321 atgaagctga ccgcatgctc gacatgggct ttatccacga tatccgtcgc gtgttaacaa
    10381 aactacctgc gaagcgccag aacctgttat tctccgcgac cttctctgac gatattaaag
    10441 ccctggcgga aaaactgttg cacaacccgc tggaaatcga agtggcacgc cgcaataccg
    10501 cgtctgatca ggtgacccag cacgttcact ttgtcgataa gaaacgcaaa cgcgaattgc
    10561 tgtcgcacat gattgggaaa gggaactggc agcaggtgct ggtgtttacc cgtaccaaac
    10621 acggcgctaa ccatctggct gaacagctca ataaagatgg catccgtagt gcggcgatcc
    10681 acggcaataa atcgcaaggt gcgcgtactc gtgcgctggc tgattttaaa tcgggcgata
    10741 ttcgtgtact ggtggcaact gacatcgctg cgcgcggcct ggatattgaa gagctgccgc
    10801 acgtcgtcaa ctatgaactg ccaaacgtac cggaagatta tgtccatcgt atcgggcgta
    10861 ccggtcgtgc ggctgctacc ggtgaagcgt tgtcgctggt gtgtgttgat gaacacaaac
    10921 tgctgcgtga tatcgaaaaa ctgctgaaaa aagagatccc gcgcattgcg attccgggct
    10981 atgagccgga cccgtcaatc aaagccgaac cgatccagaa cggtcgccag caacgtggcg
    11041 gcggcggtcg tgggcaaggt ggtggtcgcg gtcaacagca accacgccgt ggggaaggtg
    11101 gcgcaaaatc tgcaagcgcg aaacctgcag aaaaaccgtc tcgccgcctc ggcgatgcca
    11161 aaccggcagg cgaacaacaa cgtcgccgcc gtccgcgtaa acctgccgct gcgcagtaat
    11221 cttttatgcc gggctatgcc cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc
    11281 gctacgctta tcaggcctac gtgaactctg caatatattg aatttgcatg cttttgtagg
    11341 ccggataagg cgtttacgcc gcatccggca tttcacaaca agcacttgtc agcaatttga
    11401 gaacacggga aaaaatttat ctgttttacc gcccataagc caccgagacg ttacttctct
    11461 atagccaatt gttctcgcaa ctccattaaa aggtagccca gtcgattctt gcccttacca
    11521 ttaccaccgt ctccccagta agcatcgttt tccgtatgct caaccagttt tgcgggcgcg
    11581 gttgccagca agagcacacg cagttctgca tgctgttcga atttagcacg aagtgcttta
    11641 cgcatcacct gttctttaac cgactcccag tttttacgaa gtggcttgga acgattacgc
    11701 cccatgcgag cggcgaccat gggggaagaa acccgacgga tctcttcgcg gtatttttca
    11761 tcaaggaatt tttgtgcctg aaaatagtgt tcggaggtgg gccaggtttt cccgtcaacc
    11821 ttgatgggcc aggcggcaaa gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa
    11881 tttatgatgg tgtcttgcat gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg
    11941 cattatgttg gtggttattg cgagccgctt tccagaaaca gaaaaaccat tacccctgaa
    12001 aaccgaaaaa tgccacaata ttggctgttt atacagtatt tcaggttttc tcatggcatt
    12061 aaccgccgcg cttaaagcgc aaattgccgc ctggtataag gcgcttcagg aacagatccc
    12121 cgactttatt ccccgtgtgc cgcagcggca gatgattgcg gacgtcgcca aaacgctggc
    12181 cggagaagaa gggcggcatc tggcgattga agcccccacc ggcgttggga aaacgctctc
    12241 ctatttgatc cccggcatcg ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac
    12301 cgccaacgtg gcgttgcagg atcagattta cagcaaagat ttaccgctgc tgaaaaagat
    12361 cattcccgat cttaaattca ctgccgcttt cgggcgtggg cgctacgttt gtccgcgtaa
    12421 tctgacggcg ctcgccagca ctgaacccac gcaacaggat ctgctggcgt ttcttgacga
    12481 cgaactgacg gcaaataatc aggaagagca aaaacgttgt gcgaagctga agggcgatct
    12541 cgacacttat agatgggatg gtctgcgcga tcatacggat atcgctatag atgatgatct
    12601 ctggcgtcgt ttgagtaccg acaaagccag ctgcctcaac cgtaactgtt actactatcg
    12661 tgaatgcccg ttttttgttg ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa
    12721 ccatgcgctg gtgatggcgg cgatggaaag cgaagccgta ctgcctgacc cgaaaaatct
    12781 gctgctggtg ctggacgaag gtcatcacct gccagatgtg gcccgggatg cgctggagat
    12841 gagcgctgaa atcaccgcgc cctggtatcg actacagctg gacttgttca cgaaactggt
    12901 cgctacctgc atggagcagt ttcgcccgaa gaccatccca ccgctggcga tccctgaacg
    12961 tttgaatgcg cattgtgaag agttgtatga gcttatcgcc tcgttaaaca acattctcaa
    13021 tctctacatg cctgccgggc aggaggcaga gcaccgtttt gcgatgggcg aactgcctga
    13081 tgaagtgctg gagatctgcc agcggctggc aaaactcacc gagatgctgc gtggtctggc
    13141 ggagttattt cttaacgatt taagtgagaa aaccggcagc catgacattg tacgcctgca
    13201 tcggttgatt ttgcagatga accgcgcgtt ggggatgttc gaggcgcaaa gcaaactctg
    13261 gcggctggcg tctctggcgc aatcttccgg tgcgccggtg accaaatggg cgacgcggga
    13321 agagcgcgaa gggcaactgc atctctggtt tcactgcgtg gggatacgtg tcagcgatca
    13381 gctggaaagg ctgctgtggc gcagtattcc gcacattatt gtcacctccg caaccttgcg
    13441 ttcgctgaat agtttttcgc gtttgcagga gatgagtggt ctgaaagaga aagcgggcga
    13501 ccgttttgtg gcgctggatt ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc
    13561 ccggatgcgc gttgagcctt ccatcgacaa cgaagagcag catattgccg aaatggcggc
    13621 ctttttccgt aagcaggtgg agagcaaaaa acatctcggt atgttggtac tgtttgccag
    13681 cggacgggcg atgcagcgct ttctcgacta tgtgacggat ttacgtctga tgttgctggt
    13741 tcagggcgat cagccgcgtt accgtttagt tgaactgcac cgcaaacgcg tcgccaacgg
    13801 tgagcgcagc gtgctggtgg gcttacagtc atttgccgaa gggcttgatt tgaaaggtga
    13861 tctgctcagc caggtgcata tccacaaaat cgcttttccg cccatcgaca gccccgtggt
    13921 gatcaccgaa ggggaatggc tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct
    13981 gccgagcgcc tcgtttaacc tgattcagca ggttgggcga ctgattcgaa gccacggttg
    14041 ctggggcgaa gtggttatct acgacaaacg cttgctgacc aaaaattatg gcaagcgact
    14101 actggatgca ttaccggtat ttccgataga gcaaccggaa gtccctgaag gtatagttaa
    14161 aaagaaagaa aaaacgaaat ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa
    14221 ggagtcgtaa atggactatc gcaaaatcat taaagagatc gggcgcggga aaaaccacgc
    14281 gcgcgattta gaccgggata ctgcgcgcgg tctgtatgct catatgctca atggtgaagt
    14341 ccctgacctt gagttgggcg gcgtattgat tgcgctgcgt atcaaaggag aaggggaagc
    14401 agagatgctc ggcttttacg aagccatgca aaatcacacc atcaagctga cgccgccagc
    14461 aggcaagccg atgccgattg tcatccccag ttacaacggc gcgcgtaaac aggccaacct
    14521 gacgccgttg ttggcgattc tcctgcacaa actcggtttt cctgtggtgg ttcacggggt
    14581 tagcgaagat ccaacccgcg tgctgactga aaccattttt gaattgatgg gtattacgcc
    14641 aacgttgcac ggcgggcagg cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc
    14701 agtcggcgcg ttttgcccgc cgctggaaaa acaactggcg atgcgctggc ggatgggcgt
    14761 gcgtaacagt gcacataccc tggcgaaact ggcgacgcca tttgccgaag gtgcggcgct
    14821 gcgtctttcc agcgtttcgc atccggaata tattggacgc gtcgcgaagt tctttagcga
    14881 tatcggcggg cgggcgctac taatgcatgg tactgaaggt gaagtgtatg ctaatccgca
    14941 gcgctgcccg cagatcaatc tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca
    15001 ggacactgct ggtagcgagt tactgccaca agcaaaagat ccggaaacca cggcgcagtg
    15061 gattgagcgt tgccttgctg gcagcgaacc gattcccgaa tcgctgaaaa tccagatggt
    15121 ttgctgcctg gtggctatgg gtgaagcggc aactatcagc gacggcctgg cgcgcgttaa
    15181 tcaggcattt taattctttt ctcagccgga tgagccatgt catccggctt ttaccccacc
    15241 gcaatatgaa attcctgcat ctttattgac cttcccacgc ccggcgtgca gcataaaaat
    15301 acaacaaaca cataacataa acaggagtta accatggaaa gtggtcatcg ctttgatgct
    15361 cagacgctgc acagttttat tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa
    15421 gcgaaattag ttgccgatca tttaatcgcg gcaaacctgg cagggcatga ttcacatggt
    15481 attggcatga tcccaagcta tgtacgctcc tggagtcagg ggcacctgca aattaaccat
    15541 catgccaaaa ccgttaaaga ggcgggggcg gcggtcacgc tcgatggcga tcgcgcattt
    15601 ggtcaggtcg cggcacatga agcgatggcg ctggggattg agaaagcgca tcagcacggt
    15661 attgccgccg tggcgctaca taactcgcat catatcggcc gtatcggtta ctgggcggag
    15721 cagtgtgcag cggcggggtt tgtctctatc cactttgtta gcgtggtcgg tattccaatg
    15781 gtcgcgccgt tccacggtcg cgacagccgc tttggcacca atccgttctg tgtggttttc
    15841 cctcgtaaag ataatttccc gctgttgctt gattacgcca ccagcgccat tgcatttggc
    15901 aaaacccgcg tcgcctggca taaaggcgtc cccgtgccgc caggttgcct gattgacgtt
    15961 aacggcgtgc cgacgaccaa tccggcggta atgcaggagt cgccgttggg ttcgctgttg
    16021 acctttgccg aacataaagg ctacgccctt gcagcgatgt gtgaaattct tggcggggcg
    16081 ctttccggcg gtaaaacgac gcatcaggaa acgttacaaa ccagtcccga tgccattctt
    16141 aactgcatga ccactatcat catcaacccg gaactcttcg gcgcgccgga ttgtaacgcg
    16201 cagaccgaag cctttgccga gtgggtgaaa gcctcgccac atgatgatga taagccgatt
    16261 ttgctaccgg gcgagtggga agtgaacacg cgtcgcgaac ggcagaagca ggggattcca
    16321 ctggatgcgg gaagctggca ggccatttgt gatgcagcgc ggcagattgg tatgccggaa
    16381 gagacgttgc aggctttctg tcagcagtta gccagctaaa aaaaagcccg tccagtggcg
    16441 gacgggcaaa caagggtaac ataggatcaa tgagggttag agcatatgcg tctgtcggca
    16501 aacagacagg gaaatacttg tgctggacgt agcgtaaacg cctgatccgt ccaacggttc
    16561 ggattttgta ggcctgataa gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg
    16621 gcgtaaacgc cttatccgtc ctacgaatta cttataaatt accgcagtac cgcttaattt
    16681 attgttgttg gtggcggaag tgatgctgta tccactggca ccggctgccg cggctttctc
    16741 agccagtttc gcttccaggg catcgagggt ggatgcgcca tcggcagaaa ccacgccgat
    16801 tttattcatg ttctgtgcct gggatgccgt tatcggttcc gccgcgaaca cgccaaatga
    16861 cagagttgaa agagccatag cagcaacaac agtattgata gttttcataa ttaatctctc
    16921 gcaggtgatt tttgtataag tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt
    16981 acgagagata aaatcgaaga gaattgaccg ccttgttcaa ataaattgat tgatatctaa
    17041 catactgata aaccatttat ttgttgtact tatgaacttg ttttataccg cgtggcagtc
    17101 acagcagcgt taaaggcacg gaatgccacg caaaataaaa attatactcg ctatgcatct
    17161 cgccagttgt agttaacgta actggctgtc tttcgaacct ctgcgattat atcctgtata
    17221 agctggtttt tgtaaatctt tctcctgctg acaatgaata cataagcgca cgccaggaat
    17281 ggcttcccga cgggcctgcg ggatgggggc accgcactct tcacattcat ccaggctttc
    17341 gccgcgcgga atttcacccc gagcgcgggc aatcgcatct tcaattgtac tgttgatctg
    17401 ttcgttgacg gcgtcatcgt tagcccaacc ggatgccata tcgacctccc catatcaata
    17461 cttgtacagt taagtgtagc taatccaggg acgaactcgg gcagttcaag catcagatct
    17521 ccgaccattc ccgcagcaga ttatgataaa gattaagcag cgacaggatc tcttcacttt
    17581 cgccgtagcg gcttttcagc gactgaatat tgttgtccag ttcaaacagc atggcgcgct
    17641 ttttatcatc gcggatcatc gactggatcc acataaatga tgccactcgt acgccgcggg
    17701 ttacgggtgg cacgcaatgc tggctgctgg agggatacaa cacgagatcg cctgccggga
    17761 gttttacccg atgttgtccg aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt
    17821 gtggatcgct taaaaacagc gtggcagaaa ggtcagtacg catccagccg ttttgtggat
    17881 ggctgcgtac tgcgccatcc acatggaaac cataggtttc attgttctga tagcgattaa
    17941 acagcggcgt ggaaagggta cgcggcaagg ccgcggcaaa gaacaaagca tgttggttaa
    18001 ccgcgttcag cacctcattt tgcagggcgg cgtataacgt gctgcgggtg tcgacctgtt
    18061 gattgttctt aacttgcgca ccttgtgcgc cggtggtgac gcgtccatcc acccattcgg
    18121 cttgttccag ttgttcgcga aaccgggcga cgtcctgtgg cgataacacg ccgggaatgt
    18181 ggtacatcat acaagttctc caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg
    18241 catattggct gtgagcaaga aggttcttgg ctcgcccggg tgataacggt agccgctctt
    18301 gttgattgag gcgacgtaat cagtatcaaa caggttgtag acgtttagct ggaagtcgag
    18361 attgcgatta acccgatacc ccagtttggc atcggcgacc cagtaacctt cggtaaatgc
    18421 tggcgttccc accgcgccgt ctgaaccttt atgcatactg ccgatatagc gtgcgcccgc
    18481 gccaacagag atatcgtcgg ttgcctgata ttggctccat aaggtgaagg cgtgctccgg
    18541 ggtatacggc agcgatgagg aaccatcctg ggcaacatct ttgccgtttt tgatggttgc
    18601 tttttgctgg gtatagccgc caatcacctg ccacgcggga gtgatattcc cggccacgga
    18661 tatctcatag ccttcgacgc gtttcttacc gtattgcgag taagttccgt catcattttg
    18721 ctcaacttca ttttcgatat cagtacggaa cagcgcggcg gtgagcaaca gacgtttatc
    18781 cagaacctgc catttggtgc caatctcgct ggtgttggct ttttgcggtt taaaatcggt
    18841 gcggttggca ctgttaccgc tgccagactg cgcaagggcg aagttgttgc cgcccggagg
    18901 ctgctgggaa acggcatagt taatatagac attgccgttt tccgtcaggt gatacagcgc
    18961 cccggctttc cagttcatca gattgcccga cttggcggtg tcgacggtgg tgaccggaga
    19021 accttttgcc acaccagttg ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt
    19081 ggcactgtca tattcagtat gataattatc cagacggatc ccgccgttca gctcaaaatc
    19141 acgggtgatt tgcagcgtat caaaggcgta aattgcgaag gtatccgtct gaccattggc
    19201 gtttgcgccg ttgcgcgtca ggccgccggg atgaatgctg ctgtcaggat gataaatatt
    19261 taccgcgggt aacgtcaccg gattaacgcc gtagttagtc tgcgtttcac gggtaaattc
    19321 cacgccggta ctgacatcat gaccgataga accggtatag aacgtcgagg tcaggttggt
    19381 ctggttggtg agaattttat tactcacatc tttggtattc gccgtgcgtg accaggttca
    19441 gctattcaca tcgctggtgg gctgagtaat attcgacgcc ccgcccataa tcgccgtcat
    19501 caggtaatcc tgctttacgc gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt
    19561 gatgtcgtgc tcaaaacgca tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc
    19621 cgtgccgtaa aagttatgag tatcaacttt tccggaatga ttcagggccg ccgttcccgc
    19681 agatggggca gaatagcccg gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg
    19741 ctgggtgaca tgcagataat taagatacaa acgattcgct gtaccaaggc caaaagcgac
    19801 agaaggggcg acgccgtaac gctcattttt gactttgtcg cgtccggcat cgtgcgtttt
    19861 ttcgcccatt acattcaggc gcaccgcggt ggtatcacca atgacctgat tgacgtccag
    19921 cgtgccgcgg cggaaccagg cgctgccaat actggcggag gcgtcaatgc cggaatcatt
    19981 gcgcggctgt ttgctgatca tattgatcga gcccgtcggt gcgctgcgcc cgtagtcggt
    20041 gccggacggc cctttaatca cttcgacctg ctcggtattg aaggtgtcgc gcgagacgct
    20101 gccgatatcg cgaatgccat caatataaat actgttagag gtatcggcac cacgcatata
    20161 aatggcgtcg ccagtggtgg agttaccgtt ctcacccgca aaaaacgcac ccacgc
//
LOCUS       DAZPEP010000067        19763 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZPEP010000067 DAZPEP010000000
VERSION     DAZPEP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19763
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.067"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..369
                     /locus_tag="ACHUT9_002351"
     CDS             <1..369
                     /locus_tag="ACHUT9_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1348806.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
     gene            complement(403..813)
                     /locus_tag="ACHUT9_002352"
     CDS             complement(403..813)
                     /locus_tag="ACHUT9_002352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348807.1"
                     /translation="MLNIIHAKFFLLSNVVASGLNDFRPLSKVRGGGERFSAVKYCGT
                     DEYLYRGLVEKRLFHALFLQIFLYGKRVCYGLIHKEMHLGVGLKSRWLMLLCQEGQGK
                     VLYRYALAIACAADGDEMPIKKPEFDEGRTQASV"
     gene            960..1811
                     /gene="hchA"
                     /locus_tag="ACHUT9_002353"
     CDS             960..1811
                     /gene="hchA"
                     /locus_tag="ACHUT9_002353"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFR1348808.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHUT9_002354"
     CDS             complement(1919..3277)
                     /gene="hprS"
                     /locus_tag="ACHUT9_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFR1348809.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHUT9_002355"
     CDS             complement(3277..3948)
                     /gene="hprR"
                     /locus_tag="ACHUT9_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFR1348810.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHUT9_002356"
     CDS             4081..4494
                     /gene="hiuH"
                     /locus_tag="ACHUT9_002356"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFR1348811.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4603..5607
                     /gene="msrP"
                     /locus_tag="ACHUT9_002357"
     CDS             4603..5607
                     /gene="msrP"
                     /locus_tag="ACHUT9_002357"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFR1348812.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHUT9_002358"
     CDS             5608..6243
                     /gene="msrQ"
                     /locus_tag="ACHUT9_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFR1348813.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            6500..7150
                     /gene="zinT"
                     /locus_tag="ACHUT9_002359"
     CDS             6500..7150
                     /gene="zinT"
                     /locus_tag="ACHUT9_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFR1348814.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            7493..8023
                     /locus_tag="ACHUT9_002360"
                     /pseudo
     CDS             7493..8023
                     /locus_tag="ACHUT9_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(8593..8682)
                     /locus_tag="ACHUT9_002361"
     tRNA            complement(8593..8682)
                     /locus_tag="ACHUT9_002361"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8646..8648),aa:Ser,seq:cga)
     gene            8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHUT9_002362"
     CDS             8776..9573
                     /gene="mtfA"
                     /locus_tag="ACHUT9_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFR1348815.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9674..9749
                     /locus_tag="ACHUT9_002363"
     tRNA            9674..9749
                     /locus_tag="ACHUT9_002363"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9707..9709,aa:Asn,seq:gtt)
     gene            10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHUT9_002364"
                     /pseudo
     CDS             10063..17128
                     /gene="yeeJ"
                     /locus_tag="ACHUT9_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(17390..18442)
                     /locus_tag="ACHUT9_002365"
     CDS             complement(17390..18442)
                     /locus_tag="ACHUT9_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFR1348816.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            18757..>19763
                     /gene="shiA"
                     /locus_tag="ACHUT9_002366"
     CDS             18757..>19763
                     /gene="shiA"
                     /locus_tag="ACHUT9_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFR1348817.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
BASE COUNT         5514 a         4657 c         4408 g         5184 t
ORIGIN      
        1 gcaatgatgt atgctgaaac ccgcaatatg acaccatatg gtaatgtggg tatcgcaaat
       61 aaaacccaga actttgaagc tgtagcacag tatcagttcg attttggtct gcgcccatct
      121 cttgcatatg tttattcaaa aggcaaagac ctcggcggta acgactataa caacaatggt
      181 catcaagagt atgttgatca ggatctggtc aactatgttg aaattggagc gacctactat
      241 ttcaataaga acttctcaac atatgttgac tataaaataa acttgttgga taaagatgac
      301 gatttctatg acaacaatgg catcgcaaca gatgacgttg ttggtgtggg cttagtctac
      361 cagttctaat cagccaacgt gctgcggcgt gttactgtaa aactaaacac tagcctgagt
      421 tcgtccctca tcgaattcag gcttttttat tggcatttca tctccatctg cggcacacgc
      481 aattgccaat gcatatcgat acagcacctt accctgcccc tcctggcaca ataacatcaa
      541 ccagcgactc ttcaaaccaa cacccaaatg catttcttta tgaattaatc cgtaacaaac
      601 acgcttccca tatagaaata tttgcaaaaa tagtgcatga aataatcttt tctctaccaa
      661 tccgcgatat aaatattcat cagtaccaca atatttcaca gcactaaatc tctccccgcc
      721 gccccgtacc tttgataatg gtctaaaatc attgaggcca cttgcgacga cgttgctcaa
      781 taagaaaaat ttggcgtgaa taatattcaa caccagtgca gcatcatcat tacatactta
      841 cacccgccga ctaccgcttt cgatatattg actcaatttt ccagactggt taaaaatagc
      901 gaccagctca gtcgcaaata tagtgactac cctaactaag caacaataag gaatacacta
      961 tgactgttca aacaagtaaa aatccgcagg tcgatattgc tgaagataat gcattcttcc
     1021 cttcagaata ttcgcttagc caatatacca gtcccgtctc tgatcttgat ggcgtggact
     1081 atccaaaacc gtatcgcggt aagcataaaa ttctggtgat cgccgcggac gaacgttatt
     1141 tgccaaccga taacggaaaa ctgttctcga ccggtaacca tccgattgaa acgttgctgc
     1201 cgttgtatca tctccatgct gctggtttcg aattcgaagt ggcgaccatt tccggtctga
     1261 tgaccaagtt tgaatactgg gctatgccgc acaaagatga aaaagtaatg ccattctttg
     1321 agcagcataa atcgttgttc cgcaatccga agaaactcgc ggatgttgtc gccagcctca
     1381 acgctgatag cgaatatgca gcaatctttg ttcctggtgg tcatggcgca cttattggtt
     1441 taccagaaag ccaggacgta gctgctgctt tgcagtgggc aatcaaaaat gaccgttttg
     1501 ttatctccct ttgccacggc ccggcggctt ttctggcgct tcgccacggc gataacccac
     1561 tgaatggtta ttccatttgc gcattcccag acgccgcaga caaacaaacg ccagagatag
     1621 gctatatgcc gggtcatctc acctggtact tcggcgaaga gctgaagaaa atgggcatga
     1681 acattattaa tgacgacatc accgggcgag tacataagga ccgtaaagtt ctcaccggcg
     1741 acagtccttt cgcagcgaat gcgttgggta aactggcggc gcaggaaatg ctggcagctt
     1801 atgcggatta atccctgtta cgtttgaatg taaacgcata atcaatcgca gccacgttaa
     1861 tactcacgac gtggctgcgt tttttcatgg ttgatttatg gcgtactgaa gccctatgtt
     1921 aatttctttg cggtaacata atccggaaca cattatgctt attgagatag tgatacgtag
     1981 cacttccccc atgtaattcg gcaatcgctt tgactaaaga aagaccaagt ccctgaccta
     2041 cggaatgacg cgaattatct ccccgccaaa atctacggaa gagtttttca ggctcatgaa
     2101 ttttcgttcc aggactggcg acatcaatat taagatagcc gttggtatca agaaaactgg
     2161 ttatatgaat acgcgatttt tctggtgaat atctaatggc attaacaata agattcgata
     2221 acattcgttg cagtaaaatt ttatccgcaa agatttgctg attgcactcg accttaaagc
     2281 aaatctcttt ctcgtctgaa aggtattcaa gataatccaa caaattttcg acttccttat
     2341 tgagagaaag cgagtccagt ttcaccaaaa cattgttttt atctgcccgg gcaagaaaca
     2401 gtatgttctc tgttaaccgc gaaatatttt ccagctcttc aatgttaccg gcaattgttt
     2461 tttgatattc agcgatactt ctggtttgac tgagcgtaac ctgattctga cccagtaatg
     2521 cattaatggg cgttctaagt tcatgagcga gatcgtcagc aaattgactt agacgctcaa
     2581 aatctttgac taaagcatga tgcattttat tcaacgcctg cccaagaggt tttagttctc
     2641 tcggtaatgc gctaacctca acaggctccc ggctatcgtt ataattcagc gcttccgtta
     2701 caccactcaa ctttttgatc tctcgtaatc ccgttctgat taacagcgga cttaatactg
     2761 agcaaagtac aatggcgaca atgcaaatta taatgctatt aattttatac tgttcaagca
     2821 tgttatgtct ggctgaagcc aatttagcca cagtaaccgt taatgatggc gaaacttcat
     2881 caatattaat tcgtaaagca tcaatctcag tatcattaat aatgcttctg taaatgccag
     2941 ctgcgctgat tgtctcacta gcaggtatgt tatttaacat gtcatcactg acatttgtcc
     3001 ggttaacaat tttattgatg ccatcaccat gaatgatcaa gatatcctga ctaacatcca
     3061 tcatccggtt aaagtataca ggtaacgtat ctggatttac cccatcaatt aacaactgct
     3121 tgatctgtgc tgtccggtta atgagtgttg tatcatcgcg ccatttcaac tcacttgcca
     3181 ggccattata gagagtccag acaattccag cgccagcaac agacagcagc aatataaaaa
     3241 gcaaggttaa acggacggtt atagataatc ttttcattat ttttttaccg ctacgaatga
     3301 atagcccatc ccccggattg tggcgattag cttttcagga aaaggatcat caacttttgc
     3361 gcggagcctg cgaatggcga catccaccgt attggtatca ctatcaaagt tgattcccca
     3421 aatttcactc gcaataaccg ttctgggtat aatttcgcca gctctggagg ccagtagcca
     3481 aagtaactga aactccttgc gcgtcagtgt aatactgata ttgttcctgc tcacactttg
     3541 acttacagag tccattctta agccactgat ttctaatgtt gaattcaaag cgtgatgttg
     3601 ccttaattgt gcccgaaccc ttgccagcaa ctcagaaaat gaaaaaggtt ttaccagata
     3661 atcatttgct ccactgtcca gccctctgac tctgtcatcg acagaatccc ttgcagtaag
     3721 gcaaataaca ggggtttgct ttgctgttct taacgtttgt aagatctgcc agccatccat
     3781 acccggaagc ataatatcca gaatgatcaa tgcataatca tccttcagcg caagataaag
     3841 cccatctctg ccatcagaaa cggcatcaat gacataaccc gcttcggaaa gcccctgcgt
     3901 tacccactcc tgggtccttt gattatcttc aataagtaaa atcttcatga aaataatatg
     3961 ccataaatca tatgttatag aaacagccta gttcattaca aaattgtaat gctgctgtaa
     4021 ggttaccctg gccgcttttt cgctatcctc aaaactcatt cacatgacaa ggatataaac
     4081 atgttaaagc gttatttagt actctccgta gtaacggcag cattttcatt accttctttg
     4141 gtttatgccg cacaacaaaa cattcttagc gtgcacattt tgaaccagca aaccggaaaa
     4201 cccgctgccg acgtgacagt cactcttgaa aagaaagcgg acaacggctg gttacaactt
     4261 aataccgcca aaacagataa ggatggacga attaaggcac tgtggcccga gcaaactgca
     4321 actacgggcg attaccgtgt cgtatttaaa accggggact atttcaagaa acaaaatctt
     4381 gaaagtttct tccctgagat ccccgttgaa tttcatatta ataaagtgaa cgagcattat
     4441 catgtgcctt tattacttag ccaatatggg tattcaacct atcgtggcag ttaatgcata
     4501 aatatgtaaa agcagatctc tgctactgtt agggaggaca ctccctgaca gattaacagt
     4561 aaacggctct tgctggctaa cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt
     4621 ttaaaagaat cagatgttac ggccgagtcg gtattcttta tgaagcgtcg acaggtgtta
     4681 aaagcactgg gcatcagcgc agctgcactt tctttgcctc acgctgcgca tgccgatctg
     4741 cttagctggt ttaaagggaa cgatcgcccg cccgcccccg ccggaaaacc gctggagttc
     4801 agcaagccta ccgcctggca aaataatctg ccactgacgc cagtagataa agtctccggt
     4861 tataacaact tctatgaatt cgggctggat aaagccgatc ccgccgctaa tgctggtagc
     4921 ctgaaaaccg atccatggac actgaaaatc agcggcgaag tggcaaaacc attgaccctc
     4981 gatcacgatg atttaacccg tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc
     5041 gtggaggcat ggtcgatggt ggtgccgtgg attggttttc cgctgcacaa attgctggcg
     5101 cttgccgaac ccaccagcaa tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa
     5161 caaatgccag gccagcagga ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa
     5221 ggattgcgtc tcgacgaagc aatgcatccg ctcacactga tgaccgtggg tgtttatggc
     5281 aaggcgttac cgccacaaaa tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc
     5341 tttaaaggga ttaaatcgat agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc
     5401 tggaatctgg cagcgcctga cgaatacggt ttttacgcca acgttaatcc gcatgttgat
     5461 cacccgcgct ggtcacaggc taccgaacga tttattggtt caggcggcat cctcgatgtt
     5521 cagcgccagc caacgctact gtttaatggt tacgccgacc aggtggcatc gctgtatcgt
     5581 ggcctggatt tgcgggagaa tttctgaatg cgtctgacag caaaacaggt gacatggctg
     5641 aaagttagcc tgcatcttgc cggattgttg ccgtttctct ggctggtctg ggcgatcaat
     5701 cacggtggac tgggtgccga tccggtgaaa gatattcagc attttaccgg tcgcactgca
     5761 ctgaaatttt tgctggcgac cttgttaatc acacctctgg cacgctacgc aaaacagccg
     5821 ttattgatac gcactcgccg cctgttaggc ttatggtgct ttgcctgggc gacactgcat
     5881 ttaaccagtt acgcattgct ggagctgggc gtgaataatc tggcgttatt aggcaaggag
     5941 ttaattaccc ggccttattt aacgttagga attatcagct gggttatttt gcttgcttta
     6001 gcgtttactt caacccaggc gatgcagcga aaactcggca agcactggca acagttgcat
     6061 aatttcgtct atcttgtcgc gatcctggcc ccaatacatt atctgtggtc agtgaagatt
     6121 atttcaccgc agccgctcat ctacgctggg ctggctgtac tgcttttagc cttacggtat
     6181 aagaagttgc gttccctttt taagcggtta cgcaagcagg ttcacaacaa attatctgta
     6241 taacttcata ttgccgacaa agtacgcgtt atccctgccg gatgtggcgc gagcgccctg
     6301 tccggcctgc gcagatcaca tcaggttatg tttcgtttgt catcaacctc aaattatcaa
     6361 cataattcat tttatttcaa taagatagat aagtacacct gagaaaacta tgctctcatc
     6421 tcctaagagt tgttgcattt tgccatatgt tacaatataa cattacacat catatacaat
     6481 gactctggag gaaactgttt tggcaattcg tcttcacaaa ctggctgttg ctttaggtgt
     6541 ctttattgtt agcgctcctg ccttttcgca tggtcatcac tcacacggca aacccttaac
     6601 agaggtcgaa caaaaagctg ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac
     6661 gctcagtgac tgggatggag tctggcaatc cgtttatcct ttactgcaaa gtggcaaact
     6721 tgaccccgtc tttcagaaga aagcggatgc agataaaatt aaaacatttg ctgaaattaa
     6781 agattattat cacaaaggtt atgcaacaga tatcgagatg attggcattg aggacggcat
     6841 tgttgaattc catagaaata atgaaacaac atcctgtaaa tatgattacg atggatacaa
     6901 aatactcacc tataaatcag gcaagaaagg cgttcgttac ttattcgaat gtaaagatcc
     6961 tgaaagcaaa gcccctaaat atatacaatt tagcgatcat ataattgcac cacgaaaatc
     7021 atctcatttt cacattttta tgggtaatga ttcacagcaa tttttattaa atgaaatgga
     7081 aaactggcca acgtattatc catatcagtt gagtagcgaa gaagtggtcg aggaaatgat
     7141 gtctcattga gtattctcat gataacgcct cgatgccgct ttagtaagtc atcataactg
     7201 ccactggtcc atccacaaac gccactgaac gcaagctagc tacagacacg ctcatcacta
     7261 tgacgtgtct gtatattaat aagctaaccc gcattgagtt aaccaataac ggattccata
     7321 cacaatacgg ccaacagaga aagatacctg tgctcacgcc attgcttata ttggctggtt
     7381 acaatgtgca ctatcaattt tttaaataac aaattcttaa aaccacatct gtataaggaa
     7441 tttttaaggt tcgtgggata gcttgactgt aaaaattaca ggagctacaa aaatgaaccg
     7501 attctcaaaa actcaaattt atttacattg gataacgctg cttttcgttg caataaccta
     7561 tgccgcgatg gaactccgtg gctggtttcc taaaggtagt agtacttatc tgctgatgcg
     7621 agaaacacat tacaatgcgg gtatattcgt ttgggtgtta atgttttcac gcctgattat
     7681 aaaacaccgt tatagtgatc cttctattgt accaccgcca cctgcctggc aaatgaaagc
     7741 ggcttcgcta atgcacatca tgctttatat aaccttcctt gcacttcctc tgctggggat
     7801 tgctttgatg gcttacagtg gaaaatcgtg gagtttcctt ggtttcaatg tgtctccctt
     7861 tgttacccca aacagcgaaa ttaaagcact gataaaaaat attcacgaaa cctaggcaaa
     7921 tataggttac tttttaatcg cagctcacgc tggcgcagca ctctttcatc actacattca
     7981 gaaagataat actctgttac gaatgatgca tcgccgcaaa taagtcgatc ccctgacaag
     8041 gaaatgtgaa ccatgtacat caacgcgtca taaatgttta cgcaagtgca caatgcatcc
     8101 tttctactat caacaaggag gagcagaaac tgtgtaaatc gtacaatggt aagacttatc
     8161 aaaaatacag gttaattttg gtatttaaac cttttttact tgttagtatg atacaaaggc
     8221 tttcaaaaaa gctgggaaat tcaacaactc ttggaaatta tggactttgt cccgcggctt
     8281 taatctgacg gccgcgttcc ttttttgact caattattga ccccttctct acgcaacttc
     8341 agttcccacc accaactttg cggcagtttc ataggatgaa tgtctaaaag aataatggtg
     8401 agcgataaga aaacgactga ataactgcac attttcgctc gaaagcttcc tgtcagatcc
     8461 atagcgaatc aagtgctgaa tgtcacagta tcgaacagaa aacagtgacg atctaaccct
     8521 tcaagaatat tctacaattg ttctgtttag gaaaagcaag gcgggaagtc aggagataag
     8581 tcattgataa agtggcggag agagggggat ttgaaccccc ggtagagttg cccctactcc
     8641 ggttttcgag accggtccgt tcagccgctc cggcatctct ccgttcagat ggttgccatg
     8701 atgccaggaa atttggcatt ttaacagtcc ctgtccgtgc aattttgttc aagtgacgag
     8761 tttgcgagca aaacgatgat taagtggccc tggaaagtac aagaatcagc acatcaaact
     8821 gcccttccct ggcaggaagc actatcgatc ccccttttaa cgggtctgac ggaacaggaa
     8881 caaagcaaat tagtcactct tgccgaacgt tttttacagc aaaagcggct tgttccttta
     8941 cagggctttg aactggattc attaagaagc tgccggatag cacttctatt ttgcctaccc
     9001 gttctggagt taggactgga atggctggat ggttttcatg aagtcttaat ttatcctgcg
     9061 ccatttgtgg tcgatgatga atgggaagac gatatcggtc tggtgcataa ccaacgtatt
     9121 gttcagtcag gtcagagctg gcagcaaggg cctatcgttt tgaactggtt ggatatacaa
     9181 gattcttttg atgcatctgg ttttaacctg attattcatg aagtcgctca taagctggac
     9241 acccgtaacg gcgatcgcgc cagcggagtt ccctttattc cgttgcgtga ggttgctggc
     9301 tgggaacacg atcttcatgc tgcaatgaac aacattcagg aagaaatcga attggttggc
     9361 gagaatgcgg cgagcattga tgcttatgca gccagcgatc ctgctgaatg ttttgccgta
     9421 ctttctgaat atttctttag cgccccagaa ctttttgctc ctcgtttccc ttcattgtgg
     9481 caacgtttct gccaatttta tcaacaagat cctttgcaga gactgcatca cgctaatgat
     9541 acagactcgt tttcggcgac gaatgttcat taattaacaa ctttgcagat taattaacca
     9601 attgaaatga cttatgaaat ttagtgttga cagacaaggt accgctaagt aatatgtgcc
     9661 ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta atccgtatgt
     9721 cactggttcg agtccagtca gaggagccaa attctaaaaa ttcgcttttt tagcgcaatg
     9781 tcactgacct tagttgaaca ttgtttttta acggatagcg ggtttttaac accttaagcg
     9841 ccctcgacct ttatggttga gggcgttttg ctatgaacgc catcaccatt ttcccctcga
     9901 ttataaaact tgagttattc agtagtctcc cctcttgcaa ctcacaccca aaactgccta
     9961 acgaaaagtt attaattttc aatcgtattg ctatcagtat ttacattttt tcgctgtgct
    10021 agaaagggcg catttatgtt agctcgttca gggaaggtaa gcatggctac gaagaagaga
    10081 agtggagaag aaataaatga ccgacaaata ttatgcggga tgggaattaa actacgccgc
    10141 ttaactgcgg gtatctgtct gataactcaa cttgcgttcc ctatggctgc ggcagcacaa
    10201 ggtgtggtaa acgccgcaac ccaacaacca gttcctgcac aaattgccat tgcaaatgcc
    10261 aatacggtgc cctacaccct tggagcgctg gaatcggccc aaagcgttgc cgaacgtttc
    10321 ggtatttcgg tggctgagtt acgcaaactc aaccagtttc gtacgtttgc tcgaggtttt
    10381 gataatgtcc gccagggtga tgaactggat gtcccggcac aagttagtga aaaaaaatta
    10441 accccgccgc cgggtaatag cagtgacaac ctcgagcaac agatagccag tacttcacag
    10501 caaatcgggt ctctgctcgc cgaagatatg aacagcgagc aagcggcaaa tatggcgcgt
    10561 ggatgggcct cttctcaggc ttcaggcgca atgacagact ggttaagccg cttcggtacc
    10621 gcaagaatca cgctgggcgt ggatgaagat tttagcctga agaactccca gttcgatttt
    10681 ctccatccgt ggtatgaaac gcctgataat ctctttttca gtcagcatac tctccatcgt
    10741 actgacgagc gtacgcagat taacaacggc ttaggttggc gtcatttcac tcccacatgg
    10801 atgtcgggca tcaacttctt tttcgaccac gatcttagcc gttaccactc ccgcgccggc
    10861 attggcgcgg agtactggcg cgactatcta aaattaagca gtaacggcta tttgcgactg
    10921 accaactggc gcagcgcacc tgaactggac aacgattata aagcacgccc ggccaatggc
    10981 tgggatgtac gcgcagaaag ctggctaccc gcctggccgc accttggcgg taaactggtc
    11041 tatgaacagt attatggcga tgaagtggcc ctgttcgata aagacgatcg gcaaagtaat
    11101 cctcatgcca taaccgctgg acttaactat acccccttcc cgctgatgac cttcagcgcg
    11161 gagcaacgcc agggtaaaca gggcgaaaat gacacccgtt ttgccgtcga ttttacctgg
    11221 caacctggca gcgcaatgca gaaacagctt gacccgaatg aagtcgctgc acggcgtagc
    11281 cttgcaggca gccgttatga tctggtggat cgcaacaaca atatcgttct ggaatatcgc
    11341 aaaaaagaac tggttcgcct aaccctgaca gaccccgtga cagggaagtc aggagaagtg
    11401 aaatcactgg tttcgtcgct acaaaccaaa tatgccctga aaggctataa cgtcgaagcc
    11461 accgcactgg aagctgccgg tggcaaagtg gtcacaacgg gtaaagatat tctggttacc
    11521 ctgccggctt accggttcac cagtacgcca gaaaccgata acacctggcc gattgaagtc
    11581 accgccgaag atgtcaaagg caatttgtcg aatcgtgaac agagcatggt ggtcgttcag
    11641 gcacctacgc taagccagaa agattcctcg gtatcgttaa gtacccaaac attgaacgcg
    11701 gattcccatt caaccgccac actgactttt attgcgcatg atgcagcagg taatcctgtt
    11761 gtcgggctgg tgctctcgac gcgtcacgaa ggtgttcagg acatcaccct ttctgactgg
    11821 aaagataatg gtgacggaag ctatacccag atcctgacca caggagcgat gtctggcacg
    11881 ctgacgctga tgccacagct gaatggtgtg gatgcggcta aagcccccgc cgtggtgaat
    11941 atcatttctg tttcgtcatc ccgaactcac tcgtcaatta agattgataa ggaccgttat
    12001 ctctccggca atcctatcga ggtgacggta gaactgagag atgaaaatga caaacctgtt
    12061 aaggaacaaa aacagcaact gaataacgca gtcagcatcg acaacgtgaa accaggagtc
    12121 actacagact ggaaagaaac cgcagatggc gtctataagg cgacctatac cgcctatacc
    12181 aaaggcagtg gacttactgc gaagctattg gaatgaagat ttgcataccg ctggatttat
    12241 catcgacgcc aacccgcagt cagcgaaaat tgcgacatta tctgccagca ataatggtgt
    12301 actcgccaat gagaatgcag caaacaccgt ctcggtcaat gtcgctgatg aaggaagcaa
    12361 cccaatcaat gatcataccg tcacgtttgc ggtattaagc ggatcggcaa cttccttcaa
    12421 caatcaaaac accgcaaaaa cggatgttaa tggtctggcg acttttgatc tgaaaagtag
    12481 taagcaggaa gacaacacgg ttgaagtcac ccttgaaaat ggcgtgaaac aaacgttaat
    12541 cgtcagtttt gtcggcgact cgagtaccgc gcaggttgat ctgcagaagt cgaaaaatga
    12601 agtggttgct gacggcaatg acagcgccac aatgaccgcg accgtccggg atgcaaaagg
    12661 caacctgctc aatgacgtca tggtcacttt caatgttaat tcagcagagg cgaaactgag
    12721 ccaaaccgaa gtgaatagcc acgacgggat cgccacagct acgctgacca gtttgaaaaa
    12781 tggtgattat agggttacgg cctctgtgag ctctggttcc caggctaatc aacaggtgaa
    12841 ttttatcggt gatcaaagta ctgctgccct gaccctcagt gtgccttcag gtgatatcac
    12901 cgtcaccaac acagctccgc aacatatgac tgcaaccttg caggataaaa atggcaaccc
    12961 actaaaagat aaagaaatca ccttctctgt gccaaacgac gtcgcaagta agttctcgat
    13021 tagcaacgga ggaaaaggca tgacggatag taacggggtt gcaatcgcct ccctgaccgg
    13081 cacgttagcg ggcacgcata tgatcatggc tcgtctggct aacagcaatg tcagcgatgc
    13141 acagccaatg acgtttgtgg cggataaaga cagagcggtt gtcgttttgc aaacatcgaa
    13201 agcggaaatc attgggaatg gcgtggatga gacaactctg acagcaacag tgaaagatcc
    13261 gtcgaatcat ccggtggcgg ggataacggt aaacttcacc atgccacagg acgttgcggc
    13321 aaactttacc cttgaaaata acggtattgc catcactcag gccaatgggg aagcgcatgt
    13381 cacgctgaaa ggtaaaaaag cgggcacgca tacggttacc gcaacgctgg gtaataacaa
    13441 taccagtgat tcgcagccgg taacatttgt ggcggacaaa gcctcggctc aggttgtcct
    13501 gcagatatca aaagatgaga tcacaggtaa tggcgtcgat agcgcaacgc taactgcaac
    13561 ggttaaagat cagttcgaca atgaggtgaa taatcttccg gtaacattca gctcagcctc
    13621 ttcaggactc accctgaccc cgggagtaag taataccaat gagtctggca tcgcgcaggc
    13681 cactctcgca ggcgttgcct ttggtgagca gacggtcact gcatcactgg ctaataatgg
    13741 tgccagcgac aacaaaactg tgcattttat tggcgacaca gcggcggcaa aaattatcga
    13801 gttgacgcct gtcccagaca gcataatcgc cggtaccccg cagaacagct ccggcagcgc
    13861 catcaccgcc acagtcgttg ataataatgg ctttccggtg aaaggtgtga ctgtgaactt
    13921 caccagcaga acaaactctg ccgaaatgac gaatggcggc caagccgtaa cgaacgaaca
    13981 gggtaaggct accgtcactt ataccaatac ccgctcctcg atagaatcag gagcgagacc
    14041 ggataccgtt gaggccagtc tggaaaatgg tagctccacg cttagcacat caattaatgt
    14101 caacgctgat gcgtctacgg cacatctcac ctggctacag gcactttttg atacagtctc
    14161 cgcaggcgag acaaccagtc tgtatattga ggtgaaggat aattacggca acggtgtccc
    14221 ccagcatcag gtgaccctca gcgtttcacc aagtgaaggc gtgaccccca gtaataacgc
    14281 tatatatact accaaccacg acggcaattt ttacgcaagc tttaccgcta caaaagccgg
    14341 ggtttatcaa gtgacggcaa ccctcgaaaa tggcgattcg atgcaacaaa cagtgaccta
    14401 tgtgccgaac gtcacgaatg ccgaaatcac gctggcaacc tcgaaggatc cggtgattgc
    14461 cgacaataac gatctcacga cactaacagc aacagtcgct gatacagagg gcaatgcgat
    14521 agccaacact gaagtaacat ttactctgcc ggaagatgtt aaggcgaact tcacgctgag
    14581 cgatggcggt aaagcgatta ctgatgctga aggcaaagcg aaagtcacgc tgaaaggtac
    14641 aaaagcaggc gctcatactg ttacagcatc gataactggc ggtaagagtg agcagttggt
    14701 ggtgaacttt actgcggata cgctcactgc gcaggttaat cttaacgtta ccgaggacaa
    14761 ttttatcgct aataacgtcg ggatgaccag gctgcaggca acagtgactg atggaaacgg
    14821 caacccgtta gccaatgagg cggtgacatt cacgctaccg gcagatgtga gcgcaagctt
    14881 tactctcgga caaggcggtt ccgccattac tgacatcaac ggcaaggctg aagttacact
    14941 gagcggtaca aaatccggca cctaccccgt gacagttagc gtgaacaatt atggtgtcag
    15001 tgatacgaaa caggtgactt tgattgccga tgctggtacc gcaacactag cctccttaac
    15061 ctctgtatac tcattcgtcg tcagcacgac cgagggcgcg accatgactg caagcgtcac
    15121 tgacgctaac ggcaacccgg tagaaggcat aaaagttaat ttccgcggaa cctccgtcac
    15181 gctaagcagc accagcgttg aaacggatga tcggggtttc actgaaattc ttgtgacaag
    15241 caccgaagtc ggactgaaaa cagtttcagc ctctctggca gataaaccta ctgaagtcat
    15301 atcgcgatta ctgaatgcaa aagcagatat taattctgca acgattacca gtctggagat
    15361 acctgaaggt caggtcatgg tcgcacaaga cgtagcagtt aaagctcacg tcaacgacca
    15421 gtttggcaac ccgattctta atgaatctgt aacattcagt gcagagccac cagagcacat
    15481 gaccatcagc caaaatattg tctctactga tacgcatggt atagccgagg tctccatgac
    15541 gcccgaaaga aacggttcgt atatggtgaa agcatccctg gcgaatggag cctcacttga
    15601 gaaacaactg gaggctattg atgaaaaact gacactctcg gcgtccagcc cgcttatcgg
    15661 tgtcaattcc ccaacaggtg caactctgac ggcaacgcta acttctgcaa atggcactcc
    15721 agtggagggt caggtcatca actttagcgt aacgccagaa ggtgcgacgt taagtggcgg
    15781 aaaagtgaga actaactctt ctggtcaggc tccagtcgtt ctgaccagca ataaagtcgg
    15841 tacatatacg gtgactgcat cgttccataa cggcgtaaca atacagacac agacaaccgt
    15901 gaaagtcact ggcaactcaa gcaccgccca tgttactagc tttatcgctg atccatcgac
    15961 tatcgccgcc accaacagtg atttaagtac cttaaaggca acggttgagg atggcagtgg
    16021 taacctgatc gaaggtctca ctgtgtactt tgccttaaaa agcggctctg ccacattaac
    16081 gtcattaaca gcggtgaccg atcaaaacgg aatcgcgaca acaagcgtga aaggagcgat
    16141 gacaggtagc gtcacggtaa gcgcagtcac gaccgctggt ggaatgcaaa cagtagatat
    16201 aacgctggtg gcaggcccag cagacgcctc aaagtcagtc cttaagaaca atcggtcatc
    16261 attgaaagga gactttaccg atagtgctga gctacatctt gttctgcacg atatatcagg
    16321 caatccgatc aaagtttctg aagggctgga atttgtacag tcaggtacca acgtgcccta
    16381 tgtgcaagtt agtgcaattg actacagtaa aaatttctca ggcgagtaca aagccactgt
    16441 tacaggaggc ggagagggta tcacaacgct gatccctgta ttgaatggtg ttcatcaagc
    16501 tggtctgagt accacaatac aattcactcg cgcagaagac aaaataatga gcggtacagt
    16561 atcagtcaat ggtactgacc taccgacaac tacattccct tcgcaggggt tcaccggggc
    16621 gtattatcag ttgaataatg acaactttgc cccaggaaaa acggcggctg attatgagtt
    16681 ttcaagctct gcctcctggg tcgatgttga tgctaccggt aaagtgacat ttaaaaatgt
    16741 cggcagcaat tcggaaagga ttacggcgac accaaaatca ggaggcccta gctatgtata
    16801 cgaaatccgt gtgaagagtt ggtgggtgaa cgccggcgag gctttcatga tatacagcct
    16861 tgctgaaaat ttttgcagca gcaatggcta cacgctcccc agagcaaact atttaaacca
    16921 cagtagttcc cgaggcatcg ggtcactgta cagtgaatgg ggagatatgg ggcattacac
    16981 gactgaagct ggttttcaat caaatatgta ttggtcatct agtcccgcaa actcaagcga
    17041 acaatacgta gtttccctgg caacaggtga tcaaagcgta tttgaaaagc ttgggtttgc
    17101 ttatgcgaca tgttataaaa acctctgatt ttccgttata atttcttaaa gagtgctcca
    17161 ttcggagctc tcttttttac attccctcta tagagcttgc ccttctctca cagaaaatga
    17221 ataaataaaa atgcggcacc gccagaattg cgttcgatgt gactatgatc gtatccggtg
    17281 agtactgctt tgattcaaag gctggttttc tgtgatgatt gaccagcctc agagtattga
    17341 tgacaccacc gttcataaca gatcggcatt actatgcata agtactgtct catattctcg
    17401 ccaaaacact ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc
    17461 caacgacgac tgactccgag acatcggcca ttgcgggcca tcgcgttttg taatcagcat
    17521 aaattgcaac aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag
    17581 ttattgcgat ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtggtaagg
    17641 ataaacggta gacattatta taacaatcca ctaatgccct ggctttatct tcacctttgg
    17701 gtccatgaac gatcactatt ggtatatctg tttcactttg aatttttgct attagatttt
    17761 ctgcaatcga taatgaaaat gtacgttcct gcgagctacc ttctaaattg aacgcaatgt
    17821 aagatcctaa cgatcgcatt tcctcgcgca cctcatcgag tacatcctca cttagtggca
    17881 attcatatat tggcctgact gctggaaaac ccgcctcacg catcataaat gcccatgtca
    17941 taggtacggg agcccggagt ttctgatcca tacgggacgc gttcttgcac aaaggggagt
    18001 agcaattcat ggttatacca acaacctgaa aattcgtttt tgctttcaac tgactgataa
    18061 ataacatcgt tttcaggttc tttttacgca tcccctcaat acaaagatcc ggcgtaccgt
    18121 attgctgtgt tatgttcttt gctaaatctt ttatttcttt taatgttgcg tgatcctgca
    18181 tagtcattgt gactaatgtt aatttagtct gttcaagttt aagcgcatta aagacttcta
    18241 aattaattgt cgacgttaca attaaaagat gcttaatttt atgcaattca agcgcccgaa
    18301 taacaggaaa gatggccata gcatcgccaa tctgatcggg aatatggatg acaacaaagt
    18361 ctgttttttc aatattgaaa ttataagctt tataatcgta gtaactaaat gcaatacgtc
    18421 tcaacaatga tgctaaaaac atacctaacc tcgcctccct actggttata atgcaatgca
    18481 gtctatcaga ctcatcaggg tgccattttg tgcatatgcg gacttttatg tttcatatct
    18541 ctaacctgtg ggtcctctgc ttaatcctta aacaacacca gcaactcctg cgctttcatc
    18601 ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca gatgcgctct
    18661 gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt accttttcca
    18721 tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct catctccact
    18781 cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt aggcagcttc
    18841 gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcatcaccgc cgcactggtg
    18901 tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc cgcatttgct
    18961 acctttggcg tcggattcct tttccgtccg ctcggcggtg tcattttcgg tcactttggc
    19021 gaccgactgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg catcgcgaca
    19081 gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc tattttgctg
    19141 gtgacactgc gtgtcattca gggatttgcc gtcggcggcg aatggggagg cgcggcgttg
    19201 ctttccgttg aaagtgcacc gaaaaataaa aaagcctttt acagtagcgg tgtacaagtt
    19261 ggctacggtg taggtttact gctttcaacc ggactggttt cattgatcag tatgatgacg
    19321 actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag catcgtactg
    19381 gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt tgaacaacag
    19441 caacattatc aagctgccgc gaaaaaacgc atcccggtta tcgaagcgct gttacgacat
    19501 cccggtgctt tcctgaagat tattgcgcta cgactgtgcg aattgctgac gatgtacatc
    19561 gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggctaccgcg cgaacttttc
    19621 cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg ttttgcctgg
    19681 cttgccgatc gttttggtcg ccgtagggtt tatatcacag gtgcgttaat cggaacgttg
    19741 agcgcatttc ctttctttat ggc
//
LOCUS       DAZPEP010000068        19740 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZPEP010000068 DAZPEP010000000
VERSION     DAZPEP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19740
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.068"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..510
                     /gene="csgD"
                     /locus_tag="ACHUT9_002367"
     CDS             <1..510
                     /gene="csgD"
                     /locus_tag="ACHUT9_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFR1348818.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
     gene            515..904
                     /gene="csgE"
                     /locus_tag="ACHUT9_002368"
     CDS             515..904
                     /gene="csgE"
                     /locus_tag="ACHUT9_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFR1348819.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKKDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            929..1345
                     /gene="csgF"
                     /locus_tag="ACHUT9_002369"
     CDS             929..1345
                     /gene="csgF"
                     /locus_tag="ACHUT9_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFR1348820.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1372..2205
                     /gene="csgG"
                     /locus_tag="ACHUT9_002370"
     CDS             1372..2205
                     /gene="csgG"
                     /locus_tag="ACHUT9_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFR1348821.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPQAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2270..2761)
                     /locus_tag="ACHUT9_002371"
     CDS             complement(2270..2761)
                     /locus_tag="ACHUT9_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1348822.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHUT9_002372"
     CDS             complement(2863..3417)
                     /gene="ycdY"
                     /locus_tag="ACHUT9_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFR1348823.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHUT9_002373"
     CDS             complement(3441..4178)
                     /gene="ycdX"
                     /locus_tag="ACHUT9_002373"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFR1348824.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHUT9_002374"
     CDS             complement(4233..5171)
                     /gene="ghrA"
                     /locus_tag="ACHUT9_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFR1348825.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5405..5492
                     /locus_tag="ACHUT9_002375"
     tRNA            5405..5492
                     /locus_tag="ACHUT9_002375"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5439..5441,aa:Ser,seq:gga)
     gene            5537..5673
                     /locus_tag="ACHUT9_002376"
     ncRNA           5537..5673
                     /ncRNA_class="other"
                     /locus_tag="ACHUT9_002376"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(5764..6450)
                     /locus_tag="ACHUT9_002377"
     CDS             complement(5764..6450)
                     /locus_tag="ACHUT9_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HFR1348826.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHUT9_002378"
     CDS             complement(7031..7819)
                     /gene="phoH"
                     /locus_tag="ACHUT9_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFR1348827.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHUT9_002379"
     CDS             complement(8440..9711)
                     /gene="efeB"
                     /locus_tag="ACHUT9_002379"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFR1348828.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHUT9_002380"
     CDS             complement(9717..10844)
                     /gene="efeO"
                     /locus_tag="ACHUT9_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFR1348829.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10902..11732)
                     /gene="efeU"
                     /locus_tag="ACHUT9_002381"
     CDS             complement(10902..11732)
                     /gene="efeU"
                     /locus_tag="ACHUT9_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFR1348830.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12398..13906)
                     /gene="putP"
                     /locus_tag="ACHUT9_002382"
     CDS             complement(12398..13906)
                     /gene="putP"
                     /locus_tag="ACHUT9_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFR1348831.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            14328..18290
                     /gene="putA"
                     /locus_tag="ACHUT9_002383"
     CDS             14328..18290
                     /gene="putA"
                     /locus_tag="ACHUT9_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1348832.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="ACHUT9_002384"
     CDS             complement(18330..18968)
                     /gene="rutR"
                     /locus_tag="ACHUT9_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFR1348833.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            19256..>19740
                     /locus_tag="ACHUT9_002385"
     CDS             19256..>19740
                     /locus_tag="ACHUT9_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1348834.1"
                     /translation="MKIGVFVPIGHNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         5011 a         5257 c         4805 g         4667 t
ORIGIN      
        1 gacgatatat cttcaggctc tattattctt ctggatatga tggaagcgga taaaaagctt
       61 atccattatt ggcaggatac tttgagcagg aaaaacaaca atatcaaaat attgttgcta
      121 aatacgcctg aagattaccc gtaccgcgac attgaaaact ggcctcatat caacggcgtt
      181 ttttatgcca tggaggatca agaacgtgtt gtcaatgggt tgcaaggcgt cctgcgcggc
      241 gaatgctact ttacgcaaaa gcttgccagc tacttgatta cgcattcagg taactatcgt
      301 tataacagca cggaatcagc cctccttact catcgggaaa aagagatcct gaataaactg
      361 cgtatcggcg cgtctaataa cgagatcgct cgttcgttgt tcatcagcga aaatacggtt
      421 aaaacgcatc tttataatct tttcaagaag atagccgtaa aaaaccggac acaagcggtt
      481 tcctgggcaa acgataacct caggcgataa agccatgaaa cgttatttac gctggattgt
      541 ggcggcagaa tttctgttcg ccgcagggaa tcttcacgcc gttgaggtag aagtcccggg
      601 attgctaact gaccatactg tttcatctat tggccatgat ttttaccgag cctttagtga
      661 taaatgggaa agtgactata cgggtaactt aacgattaat gaaaggccca gtgcacgatg
      721 gggaagctgg atcactataa cggtcaatca ggacgttatt ttccagactt ttttatttcc
      781 gttgaaaaaa gacttcgaga aaactgtcgt ctttgcactg attcaaactg aagaagcact
      841 aaatcgtcgc cagataaatc aggcgttatt aagtacgggc gatttggcgc atgatgaatt
      901 ctaaataaaa aattgttcgg aggctgcaat gcgtgtcaaa catgcagtag ttctactcat
      961 gcttatttcg ccattaagtt gggctggaac catgactttc cagttccgta atccaaactt
     1021 tggtggtaac ccaaataatg gcgctttttt attaaatagc gctcaggccc aaaactctta
     1081 taaagatccg agctataacg atgactttgg tattgaaaca ccctcagcgt tagataactt
     1141 tactcaggcc atccagtcac aaattttagg tgggctactg tcgaatatta ataccggtaa
     1201 accgggccgc atggtgacca acgattatat tgttgatatt gctaaccgcg atggtcaatt
     1261 gcagttgaac gtgacagatc gtaaaaccgg acaaacctcg accatccagg tttcgggttt
     1321 acaaaataac tcaaccgatt tttaagcccc agcttcataa ggaaaataat catgcagcgc
     1381 ttatttcttt tggttgccgt catgttactg agcggatgct taaccgcccc gcctaaagaa
     1441 gccgccagac cgacattaat gcctcaagct cagagctaca aagatttgac ccatctgcca
     1501 gcgccgacgg gtaagatctt tgtttcggta tacaacattc aggacgaaac cgggcaattt
     1561 aaaccctacc cggcaagtaa cttctccact gctgttccgc aaagcgccac ggcaatgctg
     1621 gtcacggcat tgaaagattc tcgctggttt ataccgctgg agcgtcagag tttacaaaac
     1681 ctgcttaacg agcgcaagat tattcgtgcg gcacaagaaa acggcacagt tgccattaat
     1741 aaccgaatcc cgctgcaatc tttaacggcg gcaaatatca tggttgaagg ttcgattatc
     1801 ggttatgaaa gcaacgttaa atcgggcggt gttggggcaa gatattttgg gatcggtgca
     1861 gacacgcaat accagctcga tcagattgcc gtgaacctgc gtgtcgtcaa tgtgagtacc
     1921 ggtgagatcc tctcctcggt gaacaccagt aagacgatac tttcctatga agttcaggcc
     1981 ggggttttcc gctttattga ctaccagcgc ttgctggaag gggaagtggg ttacacctca
     2041 aacgaacccg ttatgctgtg tctgatgtcg gctatcgaaa caggggtcat tttcctgatt
     2101 aatgatggta tcgaccgtgg tctgtgggat ttgcagaaca aagcagaacg gcagaatgac
     2161 attctggtta aataccgcca tatgtcggtt ccaccagaat cctgacagat aaaaatgccg
     2221 gaattaataa tccggcattt tactgtttaa gcgaaagcgt gagtgcccct cacgctttat
     2281 ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc cacaggccgc
     2341 tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac accagtttcc
     2401 agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga acaaatgaaa
     2461 gcagcagctg tttggcctga atacacatca gaaacgccac aataccggtt atgacataac
     2521 cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc gcccacacca
     2581 cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct tgcgggcagg
     2641 caaagtaggc cgtacagccc aagaagcccg cccagcttag taagcccagg gaaacagcca
     2701 cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga agtatgttca
     2761 tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca ttttatgcaa
     2821 caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt cagaatcttc
     2881 ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca tggtgcgcca
     2941 gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc agggtaacag
     3001 atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact gatcttccag
     3061 ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg ccagtggcat
     3121 ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt acgtcctcaa cccatgcgct
     3181 acgatatggc ggcactgcac attcatcgcc gataaacaac gcattgtaat cggcagagac
     3241 ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct ccagtggcca
     3301 gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta aaggatcttg
     3361 cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact cgttcattaa
     3421 tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc cataccgcga
     3481 gattcaagga agttcagtaa gcggcgcgga gaaacattca aaatgcgctc tggcggaaaa
     3541 tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa cgcggtgtga
     3601 gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc taccgcacga
     3661 cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat ttccagcgcc
     3721 acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata tttgggattt
     3781 ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat cgcttgtgta
     3841 ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat aatcagatcc
     3901 agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt aatgttagct
     3961 tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg catgttaatg
     4021 aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat cgcaaaaagt
     4081 ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata tgcatgtgtg
     4141 ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg tattcaattc
     4201 ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg gtcgacttgc
     4261 ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta ctccacagct
     4321 tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg atgttgccag
     4381 agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc gcctttaact
     4441 ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc acgcgccagg
     4501 ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat aatgccgacg
     4561 gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag aaatgcagac
     4621 agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt tcgactccag
     4681 caacgcagcg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc cagtacgcct
     4741 gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg ttgccaatgc
     4801 gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag cacctgactg
     4861 acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa aagtggaaca
     4921 gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc aacaccggcc
     4981 ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg aggatgccag
     5041 actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac tcttgcctga
     5101 ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg gtgataaaag
     5161 atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac ttatcgttat
     5221 gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa gcagatttcg
     5281 cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt caaaaagttt
     5341 gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac aggaacgaca
     5401 atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta tacggcaacg
     5461 tatcgggggt tcgaatcccc ccctcaccgc catatttaaa gaagagctcg tacgaaagta
     5521 cgggcttttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccggggttcg
     5581 acaactggcg acagccagtt ggacagaccg tgaacgcagt gagcgggctg cccgcagggc
     5641 gagcgaagcg agtcaatccc cccctcaccg ccatctttaa agaagagctc gtacgaaggt
     5701 acgggctttt tttcgtatat tgcacacacc ggggggatga gaagccccga ccgggatact
     5761 tagttaatgc accggttcac tctttgtcgc cgcgactaat gggcgaattt tgtcacgagt
     5821 ctcccagacc ggaggaattt cctccaattt agtgacagaa gctcgtacaa aaggcacccg
     5881 tgcaagagtc ataattgtat ccaaccaacg gtcacgaata atgcgatccg ccctttcatg
     5941 actcgggtca tccagctcaa taacacagac tattttattg ttcttctttg aaaccagaac
     6001 ataatccatt gccatgccat tataaattgc aaagaaacgc tttctttctt cttcgctaaa
     6061 ctcaggttgt tctggaaagt tgatcaagcg ccccagagag acctggcaat ggatatgaca
     6121 ataatgtcca aaccattttt ccagattatc gaaatacatt ttttcacaac gcgtgattaa
     6181 ccactctttt ctttccaata aaataccttc ggcaaccatt ccctgaaata actcatctcg
     6241 ctcttgccaa cgagagtcac gataggtttt ctgcggtata tagctccttg tcgacgcctt
     6301 agcggcaggg gaagttgcag atgaagggac gggttgcgat ttagcgatga ttaccgatga
     6361 ggcaactttt cggagtgtca attttctggc aacagcaaaa ccggcgataa atgcaattgt
     6421 caaacaagtt acaaaagcca taaagctcat aattaatttt cacctatcca tgttgattat
     6481 atttcattac caatgttact cgctgcagcg tgttacatgc caacaatgca acgatcaata
     6541 tatagttagt atcatgtttt gattattaca accatcccta tttagccatt gcggcttgcc
     6601 ttccacaaaa aaataacaac acacaatatt agcaaagaat taattaagat taaccttaca
     6661 tttataatat tatccctaaa gaataaatca gaggaatata ttcggtgtta aacagtcagt
     6721 caaagtatag cccattgttg attaaacctc ataagcaaaa ttggcaccag atctcctgta
     6781 caggttgaat ggccgaaaaa agataggcag atgctcattt ttttggcttc tggaagaaaa
     6841 actctccgcg ctggcagtat tacactggtc tgatgctatg acgaaaaatg cctggtaaga
     6901 gtatctaaac cagactccag ttcagcttat caacacgctc aaagtcgccc ccctgaacgc
     6961 taacctgaag tgaaaatctg ttgaaataaa acaaagcccg gttcgcccgg gctctgcacc
     7021 gataacacac ttaactgtag gcatgcagcg tacgttggca aagtgccgaa cgtacgcagt
     7081 cctctttacc gaaccggacg atcccaacca tttcatcttc ttcgaaacgt tccagcgcgt
     7141 cacttaatcc ggagcacacg ccgcgaggca aatcgcattg cgtgatatca ccgttgacga
     7201 taaccgtcac gttctccccg aggcgggtta aaaacatttt catttgcgcg gcagtcacat
     7261 tctgcgcctc gtcaagaatg acgactgcat tttcaaaggt acgtccacgc atataggcga
     7321 acggcgcaat ttccaccttc cctatttccg gtcgcaggca gtactgcata aaggaagccc
     7381 ctaagcgccg gaccagcacg tcgtagaccg ggcgaaaata gggagcaaac ttttctgcga
     7441 tatctccagg taagaagcca agatcttcat cggcttgcag aactggacgg gtgacgataa
     7501 tcctgtcgac atccttatgt atcagggcct ctgccgcttt tgctgcgctg atccaggttt
     7561 ttccgccccc ggcttcgccc gtggcgaata tcagctgctt actctcaata gccttcagat
     7621 agtgcaattg cgcttcattt cgcgcgagga tgggcgaagt atcgcgactg tcgcgggcca
     7681 taccaatggc ttctacgccg cccatctgca caagcgaggt gaccgattct tcttcacgct
     7741 gcttatggct gcgcgaatcc cgtctcagca cacgttttgc ctcgcgacga gctttgatca
     7801 ctgctttttg tcttcccata gagagcacct tgagttgttt gtattcatca cacgcgccgt
     7861 tggcagcgcg attatgcgca cgaacatcag agggttggct tccttgtaag ccatagtttg
     7921 cttttggata aaatgccgaa aaacggctac gcgcaccgtt tacggcgtcg gtaacacatg
     7981 aaaagaaagg atgaggttga aaatgcaaag tgacgagatg actaccggag gagaaaactc
     8041 cgcgagtggt ggcgcgttga ttatctaaaa catgtccagt acaggacgtt accatccgcg
     8101 atctccatag tgactgacta tcactgccgg gaacttccgc tgctacttaa taagtacaac
     8161 agatctcgca tttattgcaa caatatattt acttatattt aactataaaa cactatttca
     8221 gtgacattag tttctactgg aaagatgaca gagtgatgac agtgatgaaa aaagctgtgt
     8281 gctttcagca ggatttgcat caggcatcag tgcacgatcg ccggatgcgg cgtgaacgcc
     8341 ttatccagcc taccaaaccc gcactgattg taggcatgat aagacgcgca agcgtcgcat
     8401 caggcatctg cgcacaaatg ccttatccgc ctaaaaacat taaacccgca ataacgcgct
     8461 tccgagataa tcgttcgcgt ccttcactcc cggcagcgaa aaaaaataac cgccgccgat
     8521 aggtttaacg tattcctcca gcgcttcgcc attgagcctt ttttgtactg tcaggaagcc
     8581 tttttccaga tcgtgttggt agcagacaaa cagcaacccc atatccagtt gtccggagtt
     8641 ggtgaccccc aacgaataac tgtagccgcg acgcagcatc aggctggact cactctccgc
     8701 cgtgcgggga ttcgccagcc ggatatggct gtccagcgca atcaccttcc cttccgggtc
     8761 gctggcgtaa tcaggcacat catgctcatg ctgcattccc agcggcgcac cggtttgctt
     8821 atcacggcca aaaatcgtct gctgttcttt cagcggcgtt ctgtcccaaa attccactcg
     8881 aaactgaatc aagcgtactg cctgatagct gccaccgatt gtccacgcag gctcctgctg
     8941 atctgccgtt acccacacca ctttttgcat caacttatca ttctggctat cgggattggc
     9001 agtgccgtct ttgaaaccca gcaaattaat cggcgtctct ttgcctttac tacgcgacac
     9061 gtgatcggaa ataaaccctt cccgcttcca gcgcacactg agcaaatccg gcgtgtgttt
     9121 gatgatatcg cgcagcgcat ggataaccgt gtcctgggtg ttggcgcaaa tctgtagcaa
     9181 cacatcacca tgacataacg ccgcatccag cgagtcgttg gggaaacgcg tcatcttctg
     9241 cagctttttt ggcatctgtg gcgcaaggcc aaagcgctca tcaaacaatg agtgacccac
     9301 cgataacgtg atggtgagat tatcgggcgc aatgtagccg ccaagaatgc cggaatcgag
     9361 tggtggcagg cgcggatttg gcgtttctgg tgctgctccg ccctgagtca gaaaagcaaa
     9421 acgctgagtc aacaagcgaa acaaccgctc aagatcggct ttatcgctgg caagcacatc
     9481 aaacgccacc agcatcattg cggcctgttg tggcgtcagg atccctgcct gatgctcacc
     9541 ataaaacggc tgtttctcat tgcgtgcatc cggtgaaagc gtgcccggtg cactttgcgt
     9601 tttttgtgca tgagcgaccg gacaacttcc cgccagcgcc agcgcaccaa tccctttcag
     9661 taaacgtcgg cgtgacggtt cattcacgcc gttttcatct ttatactgca taacgcttaa
     9721 tccagtccca gcacaccgcg aagttgcgcc agatcttccg ccagcgcagt aatcggtcct
     9781 ttcagtgcat tccggtcggc atcggtcaat ttgtcgtagg tttcaaaacc gtctttagta
     9841 cggtatttcg ccagaatggt atcgaccttt ttaaagttgg catcgacttt tgccagcagt
     9901 tccgggttgg ctttttgcag ttgtggacgc agcaaatcga caattttctg cgagccttca
     9961 acgttagcct ggaaatccca cagatcggtg tggctgtagc gatcttcttc accgctaatt
    10021 ttgctggctg ccacttcctc aatcagtccg gctgcgccgc cgaccacttt tgaaggtggg
    10081 aaagccagtt cactgatgcg tttttgcaaa tcgaccacat ctgtataaag ctgctcagcg
    10141 tactgatcca tccctttggt ggtgttgtcg ccaaacaatg ctttttccag acggtggaaa
    10201 ccagtgaatt ttgggtcggc ggctttttgc tcgtaatcat cttcacgggc gtcaatgctg
    10261 ccatccagat cggagaacag ttcagcaatc ggttcaatgc gctcatagtg ctggcgcgtc
    10321 ggcgcataca gtgctttcgc tttttcgata tcgcctgctt taatcgcgtc ggtaaaggct
    10381 ttggtgtcgg tcaccagctg cgtggtttcc gccatgacat acgctttata tgcagtaatt
    10441 gcaccaccaa gacttaacag cgcatcactt tgcgccgcat ccgccgttgc ctcacctttg
    10501 acgatcaact tccctttcgg gttagtcagc agaccgcagg tcatatcgta ttcgccaggc
    10561 tgtaaattcg ccgtcatttt ctggctaaaa ccaggggcga tattttcccg ctcttccacc
    10621 accatcacgc ctttgaggat ctcccactcc agcgccttct ggctatggtt ctgaataatg
    10681 aactgtgttt tcccggcgtt aaccgtaatg gtcatcggtt cgcactgctt atccgtcacg
    10741 gtcactttga cctgcggcac atcagcggcg ttagccataa aagcagaaga aaacagcgca
    10801 gccacgctca actgcaatgc gttacggcgg aagttaatgg tcatgacgag actatccctt
    10861 taaagtatgt tgtaactaag caagagtttg cgtcgtattt gttacgcgga gcgagacgct
    10921 gtcgcccctg cgcgtggtgg cagagcaaat gccaccagcg ccgggatgag ataaataaac
    10981 cagacggcga cttcgctgac gctcggtgct tcctgatagc caaaaatccc ttccatcagc
    11041 gtgccaaaca gcgagtgagt tgagagcact gcgctcatat cgaaggcgat ttcctgaaag
    11101 tggttccaca atccggcttc atgaaatgcg cgaatggcac cagcagccag ccctgcggcg
    11161 acgaagagaa taaacaggct ggtccattta aaaaatgcac caagattgag gcgaataccg
    11221 ccccagtaga gcaggaagcc tagcaccacg gcagtagcaa gaccgagcat tgcacccagc
    11281 ggcggccaga tcccgacatc ttgttgaaat gccgccagca ggaaaaagac cgactccagc
    11341 ccttcccttg caacggcaaa aaagaccatc atcaccagcg cccagccatg atgatttcca
    11401 cgctgcaatg cgctatcgac tgcctgctcc agttgtactt tgacgttgcg cgacactttg
    11461 cgcatccaga aaaccatcca ggtaaggatc accacggcga tcaccgccac gataccttca
    11521 aacagttcct gttctttttg cggaaattcg ccggtggttt cgttaatgaa gatacccaag
    11581 cccaggcaca acgcagcggc aagcaacacg ccaatccaca tcacgccaat ccatcggcct
    11641 cgctgggtac gcttaagata gctggcaatc aaactgacaa tcagcgcggc ttcaagtcct
    11701 tcgcgcaaca taatgagaaa cggaacaaac atgccaccca cccttaaacg tttttctgag
    11761 tcaatccatg caaagaaaag taaatcgcag tgatagcgat tatcattacg gcaaagaaaa
    11821 atacaagcgg aatgtttata aaatgatgtt ggaatgtaaa caaagagagg taataccagg
    11881 ggatataaac gattattcca ggtacaaaga tgggattgtt tacaacgagc catgtgttct
    11941 tgccagatgc cgcgtaaacg ccttatccag ccgacaaaac tgtccagact caatgcatca
    12001 cagcttccat gtaaaactga taagcgggtg tatcaagcaa tttcgtccat ccgcgattat
    12061 caaatctaac tttttgcccg gaacataata acagcgcggg tgttggtggc gtcaaaatcg
    12121 ctgaaacgtt ccccgaaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
    12181 caggacgtta cctcgcgccc gacccggtag ccgaagggga taagctgaag gtaccgcgca
    12241 gcggcgattt tgttcgccag agcccggggg tgcagggggc ggcggcgact ggccgccccc
    12301 tgcgcgctcc ctgcaccatt caatttatat ggcgaggcac attaagggag cgcaacccgc
    12361 tgctgaagcc aaacaaaacc gccgcaggct aagtccctta gttttcctgc aaccgtgacg
    12421 gcggagccga atgatagtgc gcatcggcct cggcaaagcg tttttgcatc gccgctgacg
    12481 gcgctttacc cagcaaacta aacactacaa tcccgatact gccgaagatg aagcccggaa
    12541 taatttcgta cagtcccagc cagccgaact gcttccagac gataactgtc agcgcaccga
    12601 tgatcatccc cgccagcgca ccgttgcgcg tcatgcgtga ccacatcacc gagaacagca
    12661 ccaccggacc aaacgccgcg ccaaagcctg cccacgcgta gctcactaag cccagcacgc
    12721 ggttttccgg gtttgccgcc agcgcaatcg ccaccagcgc caccaccagc accatcacac
    12781 gtcctaccca caccagctct ttctggctgg cctgtttacg cagaaacgct ttgtataaat
    12841 cttcggtaat cgcactggag cacaccagca gctggcaact taaggttgac attaccgccg
    12901 ccagaatcgc cgacagcaga atcccggcaa tccacgggtt aaacagaatt tgcgccagtt
    12961 cgataaacac gcgttcagcg ttctggttta ccgcacctgc taccgccgga tgctcgttaa
    13021 agtaagcgat accaaagaag ccaacagcca ccgcccctgc caggcaaagg atcatccagg
    13081 tcatactgat acgacgcgca tggacaatgc tgtggtgaga atctgccgcc ataaaacgcg
    13141 ccaggatgtg cggctgcccg aagtaaccca gcccccaacc catcaatgag ataatggcaa
    13201 caaagttcag ccctttgagc atatccacgt tttcgatgct cttttgtttg atcacttcca
    13261 gcgagtcacc aaagccaccg acactgataa tgacgataac cggcgtcagg atcagggcaa
    13321 aaatcatcag gctggcctgt acagtgtcag tccagctcac cgcgaggaaa ccgccaataa
    13381 aggtgtaaag gatcgtcgac gcagctcccg ccaacagagc cgtttcgtaa ctcatgccaa
    13441 aggtactttc aaacagacgc gcgcctgcca caatgcccga agcgcaataa atggtgaaga
    13501 acagcaaaat aaccagcgcg gagataatgc gcaaaatgcg gcttttatct tcaaaacgcc
    13561 cggtgaaata atccggcaat gttaaggcgt tattattgta ttcggtatgc acacgcaacc
    13621 gcccggccac cagcttccag ttgatccacg cgcctaatgt caggccaatg gcgatccagc
    13681 tttcggaaat cccggaaaga aaaaccgcgc caggcaaccc cattaacagc cagccgctca
    13741 tatccgacgc acccgccgat aatgccgtca cgaatggccc aagactacga ccgcccagaa
    13801 tatagtcgtc aaagtttttc gttgatcgcc aggcgataaa cccaatcaat atcatgccaa
    13861 agatatagac acaaaatgtc accaacatcg gtgtgctaat agccatctaa agtctccaaa
    13921 aaattattat cggcaatgtc gaaacttgcc gttatatctg ccaccggaac ggggtaacag
    13981 agtttatgtt ttaccagggc gaccgtatcc tgccggaagc gctggttatt cacaatcgat
    14041 ttaacacacc atttacatta aattttagtg ctcagcgaca ctatttttca tcaggttgca
    14101 ctctctcaca ttttttgcgg ttgcaccttt caaaaaggtt aactgccgca gagaaaaagt
    14161 ctgagttatt ttttaatcac tgtcatatcg atttctttta ttaacatttc attcattttt
    14221 aagcttgcta cgcaagtcac atttaacatg gttgcacaaa gttgcaacat catggatatt
    14281 tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg ggaaccacca
    14341 ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc gcgacacgta
    14401 tcgatcgcac accacactgg ttaattaagc aggcgatttt ttcttatctc gaacaactgg
    14461 aaaacagcga tactctgccg gagctacctg cgctgctttc tggcgcggcc aatgagagcg
    14521 atgaagcacc gactccggca gaggaaccac accagccatt cctcgacttt gccgagcaaa
    14581 tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc ctatcgccgc ccggaaaccg
    14641 aagcggtttc tatgctgctg gaacaagccc gcctgccgca gccagttgct gaacaggcgc
    14701 acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc agtggtcgtg
    14761 caggaatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa ggcgtggcgc
    14821 tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgc gacgcgttaa
    14881 ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc ccgtcactgt
    14941 ttgttaatgc cgctacctgg gggctgctgt ttactggcaa actggtttcc acccataacg
    15001 aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa aagcggtgaa ccgctgatcc
    15061 gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca gttcgtcacg ggtgaaacca
    15121 tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt tactcttacg
    15181 atatgctggg cgaagctgca ctgactgccg cagacgcaca ggcgtatatg gtttcctatc
    15241 agcaggcgat tcacgccatt ggtaaagcgt ctaacggtcg tggcatctat gaaggtccgg
    15301 gcatttcaat caaactgtcg gcgctgcatc cgcgctacag ccgcgcccag tatgaccggg
    15361 taatggaaga gctttacccg cgtctgaaat cactcaccct gctggcgcgt cagtatgata
    15421 ttggtattaa catcgatgcc gaagaggccg atcgtctgga gatctccctc gatctgctgg
    15481 aaaatctctg tttcgagccg gaactggcag gctggaacgg catcggtttt gttattcagg
    15541 cttatcaaaa acgctgcccg ttggtgatcg attacctggt tgatctcgcc acccgcagcc
    15601 gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa atcaagcgtg
    15661 cgcagatgga cggccttgaa ggctatccgg tttatacccg caaggtgtat accgacgttt
    15721 cttatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac ccgcagttcg
    15781 cgacgcacaa cgcccatacg ctggcagcga tttatcaact ggcaggtcag aactactatc
    15841 cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccactgtac gagcaggtca
    15901 ccgggaaagt tgccgacggt aaacttaacc gaccgtgtcg tatttatgcc ccggttggca
    15961 cacatgaaac gctgctggcg tatctggtgc gtcgcctgct ggaaaacggt gctaacacct
    16021 cgtttgttaa ccgtattgcc gacacctctt tgccactgga tgaactggtc gccgatccgg
    16081 tcactgctgt agaaaaactg gcgcaacagg aagggcaaac tggattaccg catccgaaaa
    16141 ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg ctggatctcg
    16201 ctaacgaaca ccgtctggcc tcgctctcct ctgccctgct caatagtgca ctgcaaaaat
    16261 ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg cccgttatta
    16321 accctgcgga accgaaagat attgtgggct ttgtgcgtga agccacgcca cgtgaagtag
    16381 aacaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccacg cctccggctg
    16441 aacgcgcggc gattttgcat cgcgctgccg tgctaatgga aagccagatg cagcaactga
    16501 ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc gaagtgcgcg
    16561 aagcggtcga ttttctccac tactacgccg gacaggtgcg ggatgatttc gctaacgaaa
    16621 cccaccgtcc attagggcct gtggtgtgta tcagtccgtg gaacttcccg ctggctattt
    16681 tcaccgggca gatcgctgcc gcactggcgg caggtaacag cgtgctggca aaaccggcag
    16741 aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt gctggaagcg ggtgtacccc
    16801 ctggcgtggt gcaattgctg ccaggtcagg gggaaaccgt gggcgcgcaa ctgacgggtg
    16861 atgatcgcgt gcgcggggtg atgtttaccg gttcaaccga agtcgctacg ttactgcagc
    16921 gcaatatcgc cagccgcctg gacgctcagg gtcgccctat tccgctcatc gctgaaaccg
    16981 gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaagtc gtcgtggatg
    17041 tgctggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcactgcgc gtgctgtgcc
    17101 tgcaagatga gattgccgac cacacgttga aaatgctgcg cggtgcaatg gccgaatgcc
    17161 ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat agcgaagcga
    17221 aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg gtgttccagg
    17281 cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt gtcgccccga
    17341 cgctgatcga actggatgac tttgccgaat tgcacaaaga ggtctttggt ccggtgctgc
    17401 atgtggtgcg ttacaaccgt aaccagctac cagagctgat cgagcagatt aacgcttccg
    17461 gctatggtct gacgcttggt gtccatacgc gtattgatga aaccatcgcc caggtcactg
    17521 gctcggccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca gtggttggtg
    17581 tgcagccgtt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc ggtccgctct
    17641 atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcagtgacg ctcgcgcgtc
    17701 aggatgcaga gtatccggtc gatgcacagt tgaaagccgc attgactcag ccgctaaatg
    17761 cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg caatatggcg
    17821 agctggcgca ggcaggaaca caacgattgc tgccggggcc gacgggtgaa cgcaacacct
    17881 ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga tgatgagcag gatgcgctga
    17941 ctcagctcgc cgccgtgctg gcggtgggca gccaggtact gtggccggat gacgcgctgc
    18001 atcgtcagct agtaaaggca ttgccatcgg cagtcagcga acgtattcaa ctggcgaaag
    18061 cggaaaatat aaccgctcaa ccctttgatg cggtgatctt ccacggtgat tcggatcagc
    18121 ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc aattgtttcg gtgcagggtt
    18181 ttgcccgtgg cgaaagcaat atccttctgg aacggctgta tatcgaacgt tcgctgagtg
    18241 tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa acaggtagcc
    18301 ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc aataataatc
    18361 cgctgcacgt tttcaaccgt ttgattgaaa aatatctcat cgcgcaacgt cgcgcctgtc
    18421 accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca aatcatgaaa
    18481 atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc ggcaatcagc
    18541 gcagatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat taacagcggc
    18601 gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc gcgggagact
    18661 tccagcttca gacggatgta ctcttttatc gccgccagcg gagcgaaatc ttcacgaaac
    18721 gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat atacagcgcc
    18781 tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa ctctgcgatc
    18841 tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc tgcgctaaga
    18901 atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt cactgcgcct
    18961 tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc cccgcccggc
    19021 ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac tgttttgacc
    19081 gtttagtcca ctttttatca gatattttag ataacgtaat attaacatat taataacatt
    19141 aactttttaa aaactggcat ccgctttgca aacaagccaa tacgcggctg aaaaggatat
    19201 gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt tgatgatgaa
    19261 aattggcgta ttcgtaccta ttggccacaa cggctggctc atttcgaccc acgcgccgca
    19321 gtacatgccg acctttgaac tgaataaagc cattgtgcag aaagcggagc attaccattt
    19381 cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg agttctggga
    19441 tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct cgcgcattca
    19501 gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc gtatggccgc
    19561 aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg gctggcaaaa
    19621 gcccgtgtat gagcagatgg gtatctggcc tggcgatgac tatttctccc gtcgttacga
    19681 ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga aaagcgattt
//
LOCUS       DAZPEP010000069        19527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZPEP010000069 DAZPEP010000000
VERSION     DAZPEP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.069"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..245
                     /locus_tag="ACHUT9_002386"
     CDS             <1..245
                     /locus_tag="ACHUT9_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFR1348835.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUT9_002387"
     CDS             complement(296..1063)
                     /gene="ygbI"
                     /locus_tag="ACHUT9_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFR1348836.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUT9_002388"
     CDS             1259..2167
                     /gene="ltnD"
                     /locus_tag="ACHUT9_002388"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFR1348837.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNNNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUT9_002389"
     CDS             2164..3426
                     /gene="otnK"
                     /locus_tag="ACHUT9_002389"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFR1348838.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDDLDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="ACHUT9_002390"
     CDS             3423..4061
                     /locus_tag="ACHUT9_002390"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1348839.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="ACHUT9_002391"
     CDS             4066..4842
                     /locus_tag="ACHUT9_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFR1348840.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="ACHUT9_002392"
     CDS             4931..6295
                     /locus_tag="ACHUT9_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFR1348841.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUT9_002393"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="ACHUT9_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFR1348842.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUT9_002394"
     CDS             complement(7444..8538)
                     /gene="nlpD"
                     /locus_tag="ACHUT9_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFR1348843.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUT9_002395"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="ACHUT9_002395"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFR1348844.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUT9_002396"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="ACHUT9_002396"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFR1348845.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUT9_002397"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="ACHUT9_002397"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFR1348846.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUT9_002398"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="ACHUT9_002398"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFR1348847.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUT9_002399"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="ACHUT9_002399"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFR1348848.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUT9_002400"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="ACHUT9_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFR1348849.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="ACHUT9_002401"
     CDS             complement(12844..13167)
                     /locus_tag="ACHUT9_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFR1348850.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUT9_002402"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="ACHUT9_002402"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFR1348851.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUT9_002403"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="ACHUT9_002403"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFR1348852.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUT9_002404"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="ACHUT9_002404"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFR1348853.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="ACHUT9_002405"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="ACHUT9_002405"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFR1348854.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   17469..17741
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17591..17619
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHUT9_002406"
     CDS             complement(17838..18131)
                     /gene="cas2e"
                     /locus_tag="ACHUT9_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFR1348855.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHUT9_002407"
                     /pseudo
     CDS             complement(18128..19051)
                     /gene="cas1e"
                     /locus_tag="ACHUT9_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
     gene            complement(19048..>19527)
                     /gene="cas6e"
                     /locus_tag="ACHUT9_002408"
     CDS             complement(19048..>19527)
                     /gene="cas6e"
                     /locus_tag="ACHUT9_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1348856.1"
                     /translation="FFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTICK
                     AGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQ
                     QLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
BASE COUNT         4606 a         5268 c         4893 g         4760 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acaacaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggcc cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgatct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt gtgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgcgcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgggca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca ggttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgatcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggca gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacacc ctgacgcgcc gcagtattta
    17641 tctgctctgg ccggtttatc cccgctggcg cggggaacac gggtgcgtgt ggctgccagt
    17701 gccggagaac ggcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt
    17761 ttttaaacaa atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt
    17821 tttaaagaac ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca
    17881 tccaaatcca ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg
    17941 atattggtcg cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc
    18001 cagatcatct cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc
    18061 agtagccaga ttgcgagccg tccacgtaag cgcggaggga cattttctgt aacaaccacg
    18121 accatgctca tccgccgcgc ccccggtgac cactatcacc cagcgtttca ggttcaggga
    18181 tggcaggcgg taacatatcc ggcgcgggtt gtggtggttc aatttcacct gcagcaagga
    18241 cttcctcaat taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac
    18301 aggctaatct gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct
    18361 ttggcacaac cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt
    18421 tgccactatg gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag
    18481 atataccgta cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt
    18541 cccagtcttt aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta
    18601 atgcatacgt ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc
    18661 gagctggcgg tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta
    18721 gatcttccgt taaagccagc tttgcctagt agagtaattt atctgcccgc gcccctccgg
    18781 gttgtccgga ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca
    18841 ccgtggcggc cagatggact gccgcgtggg aaactcttgt tcccggttcg agcataatgc
    18901 aggcgaccga tcccactgga atatgcgtgc ggatcccggt tttgtcgatc agcacgaaag
    18961 cgccgtccag tacgtcgatt tgaccgtact ggaggaagat catggaagtg cgatctttta
    19021 atgggatcgg gctcagcggt acaaacgtca cgcctctgct ccgggtttga tcagcatcag
    19081 accacaaccg aacgcccggc ttttgccata cccctgactg agacgttgca gaaataaccc
    19141 aggagcggtg accgtaagca tccccgtata atccacgcta ctgaactgga tcagttgccg
    19201 ggagttttcc cgccgcagtt gctgttgtcg gtaggcatca accgaagtat ccagcagtgt
    19261 aaaaccgctt ctctccccct gcgctgccag ccagtccagc gcagcctgtt gttgatgcaa
    19321 ccagacatca cttccttcca cctgccccct cacctgccgt ttcgcctcca tcagcaagtc
    19381 gtggcgtttg cccgctttac aaattgttgg attcgcccgc aggttgaaac aaagggattg
    19441 tccggtacgc aactctggcg caaatggtcg gcattcgatg gtgaaaatat cactttccgc
    19501 cgggcgttcc tgcgacagga caaaaaa
//
LOCUS       DAZPEP010000070        19382 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZPEP010000070 DAZPEP010000000
VERSION     DAZPEP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.070"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(32..1861)
                     /gene="glmS"
                     /locus_tag="ACHUT9_002409"
     CDS             complement(32..1861)
                     /gene="glmS"
                     /locus_tag="ACHUT9_002409"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFR1348857.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2023..3393)
                     /gene="glmU"
                     /locus_tag="ACHUT9_002410"
     CDS             complement(2023..3393)
                     /gene="glmU"
                     /locus_tag="ACHUT9_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFR1348858.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3744..4163)
                     /locus_tag="ACHUT9_002411"
     CDS             complement(3744..4163)
                     /locus_tag="ACHUT9_002411"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFR1348859.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="ACHUT9_002412"
     CDS             complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="ACHUT9_002412"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFR1348860.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="ACHUT9_002413"
     CDS             complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="ACHUT9_002413"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFR1348861.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="ACHUT9_002414"
     CDS             complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="ACHUT9_002414"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFR1348862.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="ACHUT9_002415"
     CDS             complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="ACHUT9_002415"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFR1348863.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="ACHUT9_002416"
     CDS             complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="ACHUT9_002416"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFR1348864.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="ACHUT9_002417"
     CDS             complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="ACHUT9_002417"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFR1348865.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="ACHUT9_002418"
     CDS             complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="ACHUT9_002418"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFR1348866.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="ACHUT9_002419"
     CDS             complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="ACHUT9_002419"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFR1348867.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="ACHUT9_002420"
     CDS             complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="ACHUT9_002420"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFR1348868.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="ACHUT9_002421"
     CDS             complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="ACHUT9_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFR1348869.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="ACHUT9_002422"
     CDS             complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="ACHUT9_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFR1348870.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="ACHUT9_002423"
     CDS             complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="ACHUT9_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFR1348871.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15346..16347
                     /gene="asnA"
                     /locus_tag="ACHUT9_002424"
     CDS             15346..16347
                     /gene="asnA"
                     /locus_tag="ACHUT9_002424"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFR1348872.1"
                     /translation="MKTAYIAKQRQKRQISFVKSHFSRQLEERLGLIEVQAPILSRVG
                     DGTQDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKAL
                     RPDEDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGL
                     APFLPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRA
                     PDYDDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDR
                     LQLEWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(16352..17803)
                     /gene="viaA"
                     /locus_tag="ACHUT9_002425"
     CDS             complement(16352..17803)
                     /gene="viaA"
                     /locus_tag="ACHUT9_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFR1348873.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17797..19293)
                     /gene="ravA"
                     /locus_tag="ACHUT9_002426"
     CDS             complement(17797..19293)
                     /gene="ravA"
                     /locus_tag="ACHUT9_002426"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFR1348874.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
BASE COUNT         4637 a         5127 c         4850 g         4768 t
ORIGIN      
        1 gtcggcagcc cttttttatc tatgatagtg attactcaac cgtaaccgat tttgccaggt
       61 tacgcggctg gtcaacgtcg gtgcctttga tcagcgcgac atggtaagcc agcagctgca
      121 gcggaacggt gtagaagatc ggtgcaatca cctcttccac atgcggcatc tcgatgatgt
      181 gcatgttatc gctacttaca aaacccgcat cctgatcggc gaagacatac aattggccgc
      241 cacgcgcgcg aacttcttca atgttggatt tcagtttttc cagcaattcg ttgttcggtg
      301 caacgacgat aaccggcata tcggcatcaa taagcgccag cggaccgtgt ttcagttcac
      361 cagcagcgta ggcttcagcg tgaatgtagg agatctcttt cagcttcaat gcgccttcca
      421 gtgcgattgg gtactgatcg ccacggccca ggaacagtgc gtgatgtttg tcagagaaat
      481 cttctgccag agcttcaatg cgtttgtcct gagatagcat ctgctcaata cggctcggca
      541 gcgcctgcag accatgcaca atgtcatgtt caatggaggc atccagacct ttcaggcgag
      601 acagcttcgc caccagcatc aacagcacag ttaactgagt ggtgaatgct ttagtggacg
      661 ccacgccgat ttccgtaccc gcgttggtca tcagcgccag atcggattcg cgcaccagag
      721 aagaacccgg aacgttacag attgccagcg aaccaaggta acccagctct ttcgacagac
      781 gcaggcccgc cagggtatcc gcggtttcgc cagactgtga caaggtgatc atcaggctgt
      841 tacgacgcac ggcagattta cgatagcgga attcagaggc gatttcgacg tcgcacggaa
      901 tacctgccag cgattcaaac cagtagcggg aaaccatacc ggagttataa gaagtaccac
      961 aggcgaggat ctgaatatgc tcaaccttcg acagcagttc gtcggcgttc ggtcccagct
     1021 cgcttaaatc aacctgaccg tggctgatgc gcccggtaag ggtgtttttt atcgcgttcg
     1081 gctgttcgta gatctctttc tgcatgtagt gacggtaaat acctttatcg cccgcgtcat
     1141 attgcagatt ggattcgata tcctgacgtt ttacttccgc gccagtttta tcgaagatgt
     1201 ttaccgaacg gcgagtgatt tccgcaatat cgccctcttc aaggaagata aagcgacggg
     1261 tcaccggcaa cagcgccagc tggtcagaag cgataaagtt ttcgcccata cccaagccaa
     1321 tcaccagcgg actaccagaa cgtgccgcca gcagggtatc cgggtgacgg gagtccatga
     1381 tcactgtacc gtacgcaccg cgcagctgcg ggatagcacg cagaacagcc tcacgcagag
     1441 tcccgccttg tttcagctcc cagttcacca gatgggcaat cacttcggtg tcggtttcag
     1501 aaacgaaggt atagccacgc gcttttaact cttcacgcag cggttcatgg ttttcgatga
     1561 tgccgttatg caccaccaca atgtgttcag aaacatgcgg atgcgcgtta gcttctgaag
     1621 gttcaccgtg ggtcgcccag cgagtgtgag caataccggt gccgccatcc agaggatgtt
     1681 cttccgctgc ctgagccagc atctggactt taccgaggcg acgcaggcgg gtcatatgac
     1741 cttccgcatc aacaacggcc agaccggcag agtcatatcc gcggtattcc agacgacgta
     1801 aaccttcaag aaggatttct gctacatcac gttgcgcgat cgcgccaaca attccacaca
     1861 tagtttttga ttccgattta tatcgttgtc ggtcaacctg tatgcccgtt ttttcgggcg
     1921 ccccgagcct tgtagagagt ggggttattt ttatagttac tgcttgtggg agggagatta
     1981 tgttatctcc tcatcccaga tgaccggatt acccggccag aattactttt tctttaccgg
     2041 acgacgccag ccttctttct gagtctgcgg cacacggctg atagctaatg cattttcgcc
     2101 gacattacgc gtcacagttg tacccgcagc aatggtcgcg cctttgccta ctgttaccgg
     2161 ggccaccagc tgagtgtcgg aaccgacaaa cacatcgtcg ccgataatgg tcttaaattt
     2221 attcgcacca tcgtagttgc aggtaatggt tcccgcgccg atgttaacgt tatcgccaat
     2281 ttccgcatcg cccaggtaag tcagatgccc gactttcgag cctttaccca gacgcgcttt
     2341 tttcatctca acgaagttac caacgtgtgc accttccagc aactcagcac caggacgcaa
     2401 acgagcaaac gggccaatag tacaggccgc tgccagattc gcgtcttcca caacggtata
     2461 cggactgatt tcgcaatcat cgccaatcac gctgttttta atcacgcaac cggtgccgat
     2521 tttcacgcga tgaccgagag tcacgttgcc ctcgatgata acgttagtat caatttcaac
     2581 atcgcgcccg tgagtaagcg taccgcgcag atcaaaacgc gccggatcgc gcagcataac
     2641 gcctgctaac agcagttttt cagcctgttc ggactggtaa acgcgctcca gacgggagag
     2701 ttgcaggcgg ttattcacac cttctacttc gcttaaacgt tgcggatgaa cggcgacgat
     2761 ttcacgccct tcctgatacg ccagcgcaat aatgtcggtg atgtagtatt cgccctgagc
     2821 attattgttg gtcagcttcg ccagccagcg tttcatatct gcgccgttag cgatcagaat
     2881 gccggtgttg atctcctgaa tctgacgctg ctcgtcggtg gcatctttgt gctcaacaat
     2941 gccggtaact ttgccatttt cacgggtgat acgtccataa ccggtcggat catccagttt
     3001 caccgtcagc agaccaatgc caccctgcgg tttagcatca cgcaggcgct ggagtgtttc
     3061 gacagagatc agcggcacgt cgccgtagag cattaaaatg tcttcatcat cggcaaagaa
     3121 aggtgcggcc tgctgcattg catgacccgt acccagctgc tctgcctgaa gcacccagtt
     3181 aaggttgtcg tctttcagcg cctgtttaag cagatcgccg ccgtgaccgt acaccaagtg
     3241 aacgtgcgct gcgcctaatt cattcgcagc atcaatgaca tgctgaacca tcgctttccc
     3301 ggcaagggta tgcagcactt tcggaagatc ggaatacatg cgcgtgcctt tgcctgcggc
     3361 aaggatcact acgctcatag cattattcaa catacgcgtc ctgactgtaa tttgagaacg
     3421 aatttaaacc gcttcacctt gaaaaaacta catttttttc atcgtgaaat ggacagagga
     3481 taaattgttc aataacggat tatccccgct gacgaaagtg ccattttcga ccatcgtcac
     3541 gccttttgtc tgcagaaaat taaggcagta ccttcattta agcgtcaggt ggatgttttt
     3601 gctcttattt cgatcaatga ataaacggga aaacaggggt gttttatatc agcaggatct
     3661 atgtgaacgc tcttcaggac ggatcacgcg taaaaaaaag ccagcctgtt tccagactgg
     3721 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
     3781 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
     3841 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
     3901 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc caggctgcac ttcaagaatg
     3961 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
     4021 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
     4081 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
     4141 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
     4201 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
     4261 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
     4321 ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct
     4381 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaagatgg
     4441 cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
     4501 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
     4561 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
     4621 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg
     4681 cctgtacgga ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca
     4741 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
     4801 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
     4861 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
     4921 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
     4981 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
     5041 cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt
     5101 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
     5161 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
     5221 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
     5281 ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga
     5341 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
     5401 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
     5461 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
     5521 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
     5581 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
     5641 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
     5701 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
     5761 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
     5821 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
     5881 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
     5941 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtaa gcctgcaaca tcacttttac
     6001 cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac
     6061 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
     6121 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagt agttttttga acaggttaat
     6181 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
     6241 gtcgcggtct tccaggtaag ggtgcttata ttccagatta ccgtgtgcaa ggtgaccaat
     6301 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
     6361 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
     6421 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
     6481 cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
     6541 atgcctttca gcttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
     6601 atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt
     6661 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc
     6721 tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg
     6781 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
     6841 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
     6901 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
     6961 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
     7021 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga
     7081 gaaccggttt tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca
     7141 cgtgcagcac gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca
     7201 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
     7261 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg
     7321 caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
     7381 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
     7441 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
     7501 atggcatcga tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc
     7561 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
     7621 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
     7681 tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc
     7741 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg
     7801 gcaaggtcag cgtacggacc cataacaacc gcacctacag agtcgcgctc aaggttcagt
     7861 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
     7921 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
     7981 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
     8041 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
     8101 cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac
     8161 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
     8221 attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta
     8281 gcctcactca cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga
     8341 cgaccatttt cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
     8401 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
     8461 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
     8521 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
     8581 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
     8641 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
     8701 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg
     8761 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca
     8821 gaatctgcga gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt
     8881 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
     8941 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
     9001 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
     9061 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
     9121 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
     9181 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
     9241 ggaatcagat caggttgacg cgctgcgcct tccaggaatt tacccccgag gatgccgata
     9301 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
     9361 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
     9421 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
     9481 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
     9541 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
     9601 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
     9661 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
     9721 gaagccgccg atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccag
     9781 tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc
     9841 cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat
     9901 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
     9961 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    10021 cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa
    10081 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    10141 ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    10201 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    10261 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    10321 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    10381 agaactttga aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta
    10441 tacgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    10501 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    10561 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    10621 gacacagaca tcacgttttt cactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    10681 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gtgcgccccc
    10741 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    10801 tacaattgcg acttttccgc taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    10861 acactagtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagtatattt
    10921 atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact
    10981 gttttaataa atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt
    11041 aacagccaat gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact
    11101 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tacaaaagag tgaacgtggc
    11161 gttaaatgta accagttata tcagtagaaa acttggttgt tgttaacagt ctaaccggtc
    11221 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    11281 acgttcgcca tccagagctg gaacctgaag tttaaccact gattcgacct gatattcttc
    11341 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    11401 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    11461 aattacgcca tcaaatggcg gctctgaagg aaactcttct accctgctct gtactggctc
    11521 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    11581 gctatccaac agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag
    11641 tcctggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    11701 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    11761 cgctttgttc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    11821 ggtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    11881 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    11941 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg
    12001 cgtgacgcca gaaatacgcg aagcctggcc gatagaggcc ggtttgtgat cgttaagttt
    12061 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag
    12121 ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    12181 gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa
    12241 cggcgtcagc gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc
    12301 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    12361 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    12421 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    12481 caaacgcaga tccgcattat cttcgcgcag catcagacga tattctgcgc gcgaagtaaa
    12541 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaaccagta cgccgagata
    12601 cgcctgagaa cgcgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    12661 accgaccagc aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    12721 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgtggatc
    12781 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    12841 catggagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    12901 ataaatttcg ttagaggtca gcccttccgg ttcaaggaag atctgatgct gatttctgtc
    12961 ggcgaagcgc atgactttgt cttcgatcga tgggcagtag cgtgggccga caccttcgat
    13021 cacccctgcg tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc
    13081 gttggtatga gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa
    13141 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt
    13201 tcgagcatca atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc
    13261 acgcaaacgg cgagaaagcg gaatggacgg cggatcacca gcacgaccac cgctgtaatt
    13321 atccagaccg atatgaattt taccgtcgag gaacgtccca acggtgagca cgacggcttt
    13381 ggcacggaac ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat
    13441 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    13501 taccgcctga cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc
    13561 tttgcttgcg tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag
    13621 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    13681 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    13741 catacgcgcc gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    13801 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    13861 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    13921 ggtattaaaa agaagatcta tttatttaga gatctgttct attgtgatct cttattagga
    13981 tcgcactacc ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc
    14041 attaactgtg aatgatcggt gatcctagac cgtataagct gggatcagaa tgaggggtta
    14101 tacacaactc aaaaactgaa caaccgttgt tccttggata actaccggtt gatccaagct
    14161 tcctgacaga gttatccaca gtagatcgca cgatctgtat acttatttga gtaaattaat
    14221 ccacgatccc agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat
    14281 cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc
    14341 gatagcccca caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac
    14401 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    14461 aatatctccg gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc
    14521 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    14581 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    14641 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat
    14701 ctacctgtgg aaaagtatgg gattaaaaaa gccgattatg gcttgatggt acgcatgatc
    14761 gggttttgca ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg
    14821 ttgataagta catgctggag agcgtcgatc gaacggcaca ttacttttat aaagatgctg
    14881 tagtggcctg ttgtgtaata ggcttcagtg acttcagcaa ggctttccag ctttgccagc
    14941 gcggaagggt agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    15001 ccgagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    15061 ttctctactc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    15121 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    15181 agataatttt ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct
    15241 tttttaatga atcaaaagtg tgttaggctt tttattgaat gattattgca tgtttgtcgg
    15301 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    15361 attgccaaac aacgccaaaa acgccaaatt agcttcgtga aatctcactt ttctcgccaa
    15421 ctggaagaac gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat
    15481 ggcacgcagg ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg
    15541 cctgatgccc agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg
    15601 caacacgact tcagcgcggg cgaagggctg tacacgcaca tgaaagccct tcgccccgat
    15661 gaagaccgtc tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta
    15721 atgggcgacg gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg
    15781 ggaattaaag caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg
    15841 gatcagatcc acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc
    15901 aaagggcgtg agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc
    15961 ggcaagctga gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc
    16021 accccgtcag agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta
    16081 ctggaagatg cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag
    16141 catcagttgg cgctgaccgg tgatgaagat cgtctgcagc tggaatggca tcaggcgctg
    16201 ctgcgcggtg aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg
    16261 ctgctgctgc aactgccgca tatcggccag gttcagtgtg gtgtatggcc agctgctgtt
    16321 cgcgagaacg tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc
    16381 ttcgcatccc ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc
    16441 cgtgtgccga catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca
    16501 ctttactcgt cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcat
    16561 cggcatcaaa ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa
    16621 gatcggtgcc gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttggg
    16681 ggcctgaaag ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt
    16741 tttcagcgag agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa
    16801 agccgcccat tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat
    16861 cgtaatcttt atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca
    16921 ggcgataggt gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg
    16981 ttatccctag tgtcgccagt tctggcggca gaagacgtaa aatatcatcg ctttgttgca
    17041 gaccatcaac ctgctcagga accgtcgccg gttcgcgcac catggtgcgg aaggtttcca
    17101 tctgcgcatc gttgcgcggt attgatttgg cttcccgaga acgccccagc tgctctgcca
    17161 ggcgtttcag ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt
    17221 cgccacgttt aagctgaccg gcgctcatat cccacagacg accagctgca gtattgttat
    17281 ctgcgagaat cggttcaagt tgtccgctca gcgtcatgcg ttcttgaact tcactcaaca
    17341 gttgttcgcg ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac
    17401 ttaaacgcca acgctggaga aaaagcgtgt gtaacgctga agttattgtg ctgttagcat
    17461 caaccaactg ccgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga
    17521 tctgtggtag ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca
    17581 tcacctcttc ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct
    17641 cacgccagcg gggaacatca tcagtgattg ccgccttcag gcgtgggaat ttttcaaaga
    17701 agaccgccag ctgcggtgag gccagcagtg cgatgatcat ctcttcgatc aatccctctt
    17761 cgctgacggc cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggtgaat
    17821 ctgtgcaccg acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa
    17881 cagacatttt tgctgttcgc tgaataaagc gtgttgcttg cgccagtcgc tttcaagctc
    17941 ttccagttgt tgttttattt caccaggcag accttcagcc gatgaaccgg gaagtgccag
    18001 cgtactgcct tgtaaactta catcgcgaac gacaagatgt tgggcgctat caacttccag
    18061 attcagtttc tgggcaaagc cgataccgtt cagtttaccg cgaatttcac cacctttgct
    18121 cagccactgt tccagcgcgc tacgctcaaa ggagatatga accacttcca tatcatgcag
    18181 ttttaacggt ttttgcagca gtagagtcag agtggaagca gtaacgttaa caggaagttg
    18241 atactgctga cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc
    18301 gctttgttgc tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat
    18361 cccttgctgt tgccaggcat gaccggtcat caatacatca atttgttgtt gtatcaaatt
    18421 caggctttgc gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac
    18481 agcactgcga ccgctaaaaa aggcgctggc ctgcaataat cggatcgctt ttttccagcg
    18541 acgatccgag acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat
    18601 cagctcaaat acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata
    18661 ttcttcatct gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact
    18721 ggtcagcatg gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag
    18781 catgcggtca tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc
    18841 caccagcaga cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt
    18901 aatggcggtg agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa
    18961 gacaatttct gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt
    19021 tagcgcctga atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag
    19081 atattcaaac gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt
    19141 ggcaatacct ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca
    19201 caagcggatg gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga
    19261 aattctttcc gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag
    19321 ttctgcgtaa attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg
    19381 gg
//
LOCUS       DAZPEP010000071        19050 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZPEP010000071 DAZPEP010000000
VERSION     DAZPEP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19050
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.071"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..468
                     /locus_tag="ACHUT9_002427"
     CDS             <1..468
                     /locus_tag="ACHUT9_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1348875.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="ACHUT9_002428"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="ACHUT9_002428"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFR1348876.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="ACHUT9_002429"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="ACHUT9_002429"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFR1348877.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUT9_002430"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="ACHUT9_002430"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFR1348878.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="ACHUT9_002431"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="ACHUT9_002431"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFR1348879.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="ACHUT9_002432"
     CDS             complement(4084..5313)
                     /locus_tag="ACHUT9_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFR1348880.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="ACHUT9_002433"
     CDS             complement(5377..5817)
                     /locus_tag="ACHUT9_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFR1348881.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="ACHUT9_002434"
     CDS             complement(5922..6692)
                     /locus_tag="ACHUT9_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1348882.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="ACHUT9_002435"
     CDS             complement(6689..7615)
                     /locus_tag="ACHUT9_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1348883.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="ACHUT9_002436"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="ACHUT9_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFR1348884.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUT9_002437"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="ACHUT9_002437"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFR1348885.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUT9_002438"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="ACHUT9_002438"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFR1348886.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUT9_002439"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="ACHUT9_002439"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFR1348887.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEIPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="ACHUT9_002440"
     CDS             13322..13669
                     /locus_tag="ACHUT9_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFR1348888.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="ACHUT9_002441"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="ACHUT9_002441"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFR1348889.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="ACHUT9_002442"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="ACHUT9_002442"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFR1348890.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUT9_002443"
     CDS             complement(15489..15851)
                     /gene="yacL"
                     /locus_tag="ACHUT9_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFR1348891.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUT9_002444"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="ACHUT9_002444"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFR1348892.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
BASE COUNT         4706 a         5124 c         4653 g         4567 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcaagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtattt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg
//
LOCUS       DAZPEP010000072        18842 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZPEP010000072 DAZPEP010000000
VERSION     DAZPEP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.072"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..101)
                     /locus_tag="ACHUT9_002445"
     CDS             complement(<1..101)
                     /locus_tag="ACHUT9_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348893.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTA"
     gene            complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUT9_002446"
     CDS             complement(252..2123)
                     /gene="ppiD"
                     /locus_tag="ACHUT9_002446"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFR1348894.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUT9_002447"
     CDS             complement(2315..2605)
                     /gene="hupB"
                     /locus_tag="ACHUT9_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFR1348895.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUT9_002448"
     CDS             complement(2796..5150)
                     /gene="lon"
                     /locus_tag="ACHUT9_002448"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFR1348896.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUT9_002449"
     CDS             complement(5338..6612)
                     /gene="clpX"
                     /locus_tag="ACHUT9_002449"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFR1348897.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUT9_002450"
     CDS             complement(6738..7361)
                     /gene="clpP"
                     /locus_tag="ACHUT9_002450"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFR1348898.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUT9_002451"
     CDS             complement(7607..8905)
                     /gene="tig"
                     /locus_tag="ACHUT9_002451"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFR1348899.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKRALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHUT9_002452"
     CDS             complement(9249..9566)
                     /gene="bolA"
                     /locus_tag="ACHUT9_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFR1348900.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9871..10449
                     /locus_tag="ACHUT9_002453"
     CDS             9871..10449
                     /locus_tag="ACHUT9_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1348901.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10493..11968
                     /gene="ampG"
                     /locus_tag="ACHUT9_002454"
     CDS             10493..11968
                     /gene="ampG"
                     /locus_tag="ACHUT9_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFR1348902.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHUT9_002455"
     CDS             12429..13376
                     /gene="cyoA"
                     /locus_tag="ACHUT9_002455"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFR1348903.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHUT9_002456"
     CDS             13398..15389
                     /gene="cyoB"
                     /locus_tag="ACHUT9_002456"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFR1348904.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15379..15993
                     /locus_tag="ACHUT9_002457"
     CDS             15379..15993
                     /locus_tag="ACHUT9_002457"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFR1348905.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15993..16322
                     /locus_tag="ACHUT9_002458"
     CDS             15993..16322
                     /locus_tag="ACHUT9_002458"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFR1348906.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHUT9_002459"
     CDS             16334..17224
                     /gene="cyoE"
                     /locus_tag="ACHUT9_002459"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFR1348907.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17409..17720
                     /locus_tag="ACHUT9_002460"
     CDS             17409..17720
                     /locus_tag="ACHUT9_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFR1348908.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17704..18246
                     /locus_tag="ACHUT9_002461"
     CDS             17704..18246
                     /locus_tag="ACHUT9_002461"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1348909.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18302..>18842)
                     /locus_tag="ACHUT9_002462"
     CDS             complement(18302..>18842)
                     /locus_tag="ACHUT9_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFR1348910.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4214 a         4956 c         4581 g         5091 t
ORIGIN      
        1 gccgtcggtt tcgccacaga agctgccgcc agcgcgctat gagacattcc ggcacaggcc
       61 agggacaggg taatgagcag tgctttaatt ccgtgtttca tactgttttc tccttgtttg
      121 ttaacagtgg aatcacctta acgggagtga aaacagtaaa caaatggcaa tgttcagaaa
      181 tggaaaaggc cgcgaaagcg accttttctt gttacagctg caacgcattt ttttgcgagg
      241 cggcttcagg attattgctg ttccagcgca tcgccaattt tgattttcgc ctctttacgc
      301 aggttactca tcagagcttc aaagacgatt tgtgcgttgt tctgggtgat accctgcacc
      361 atcgcttttt tctgatcttc cggcattgaa ccttgtttca cttcatccag cgccagcaga
      421 accacattac cttgcatatc ggtcgccata ccgtagctcg gtttgtcttt cgctggcagt
      481 ggcagtgcaa acgccgcctg gctaatcggg tcacgaccgg aacggcttaa ggttttcggc
      541 tcgccaaatt tcagaccggc agcctgcata gcttccgccc ctttgccggc tttcaaatca
      601 accagcagtt tctcagcatc cactttcgcc tgttgttcag ctttgttgtg ctgaaccagt
      661 gccttaactt gttcctgaac atctgccaac ggtttcaccg cttccggttt gtgctcgctg
      721 atgcgcagca cgaatgcgcg gtcgccgtct acggtgatga tgtcagagtt gatgcccggc
      781 gcgccgtttt cacctaccag accgccgtta aagatagcgt cggcaaccgg cttgaagttc
      841 aactcttccg gcaggttatc tttgctgaac caacccgtct gagtggcttt aacgccggca
      901 gcttgctctg caccggccag agactcggtg tcgttgcttg ccgcatcgct cactttctgc
      961 tgcagcgcat agtacgcatc gagggctttt tcgtgtttca ctttcgccgc aatgtcgtca
     1021 cgtacttcgt ctaacgattt cactttcgct ggctgaatgt cgtccagacg tacaatcagg
     1081 aaaccgaccg aagatttgat gacaccagac agttggcctt tttctttcag accagcattt
     1141 ttcagttcgt cggggatagt ggcatcttct aaccaaccca tatcgccgcc gttacgggca
     1201 gagataatat cggcagattt ttctttggct aatgcagcaa aatcaccgcc tttattcagc
     1261 tcatcaagta ccgctttcgc ttcatcttca gttttggtct ggatgatgct gtagcgggta
     1321 cgctgcggct gggtgaattg atcctgatgc tgatcgtagt agctctggat atccgcatcg
     1381 ctaaccggtt gctgcatcgt tgcggcatcc agcttgatgt aactcacgcg gaattgttcc
     1441 ggcgtcatga aattgttttt gttttgttcg tagtagctgg caatctcctg ttcggtcaca
     1501 ggctgcttcg ccgccagcgc gttaacatcg atagtcgcct cacgcaccac gcgttgttgc
     1561 gcgaccagtg ccgccagctc gtcggtttca cctttcagca taaaatcggt accggcaaca
     1621 ccgttaatca gctgttgggt agtgagctgg ttacgcagcg cctgggcgta ctgatcggcg
     1681 gtcatcccca tctggttgag gataccgtta tagcggctgt tatcaaattt gccgtcaacc
     1741 tggaaggctg gggtcgcgaa aatcgcctgt ttaacctgct catcactgat acccagtttc
     1801 agctcacgag cgtactgatc caacagcgcc tcgtcgatca gacgattcag cacctgttga
     1861 cgcagggttt tcatatagcc ttcgttcgct gccagctcgg agtattgatc gcccagctgt
     1921 tgctgcatgc gattacgctc gctgttaaag gcgttctcga actgcccacg gctgatttcc
     1981 tggtcattca cttttgcggc gtagttattg cctccgccaa tcaggtaacc actcacgccg
     2041 gtcaatatga acgacacgat aatgataccg aaaataatct tgagcacgag actgtttgca
     2101 gccgtgcgta agctgtccat catggtgtaa caacactccg ctgtagatga ctgtgtacct
     2161 cacgcaacgt atcgatgcgc gcaaaggcct attgtgacaa gaaacggggg caattgtcag
     2221 cccacaagac acagaaagtc accgaataca aataaaaaag gcacatcagt agatgcgccc
     2281 ttgaacttcg tcacatcccc actggggaca acgcttagtt taccgcgtct ttcagtgctt
     2341 tacctgcacg gaagctcggt actttagcgg cagcgatggt gatctcttta ccggtctgcg
     2401 ggttgcggcc agtacgggca gcacgctctt taacggcaaa agtaccaaaa cctaccagtg
     2461 ctacatcatc cccttctttc agagattcag ttacggaagc aataatagca tctaacgcac
     2521 ggccagccgc agctttagag atatcagccc ctgcagcaat cttgtcgatc aattgagatt
     2581 tattcactct tctcttcctc tttataattt atatcgcact tgaatccttc aaggtacgaa
     2641 cgcgcagcag ttatatcagg ccagccatcc ccttacaaca ccccttaata agggcaagcc
     2701 cgatccgcca tctaacttag cgagacaaaa aaaggctggc aagcccgaat tagcctgcca
     2761 gccctgtttt tattagtgca ttttgcgcga ggtcactatt ttgcagtcac aacctgcatg
     2821 ccagacggtt cattttgcag cgccagagtc agaacttcct caatgcgctt cacaggatga
     2881 atgtccagat cggcaattac gttgtcagga atctcttcca gatcgcgttt attttcgaac
     2941 ggaattagca ctgttttaat cccgccgcga tgcgctgcca ggagtttttc tttcaaacca
     3001 ccgatcggca gtacctgacc acgcagagtg atctcaccgg tcattgccac atcggcacga
     3061 accgggttac cggtcaggca agaaaccagc gcggtgcaca tagcaatacc ggcactcgga
     3121 ccatctttcg gcgtcgcacc ttccggtacg tggacgtgga tgtcacgttt ttcgtaaaaa
     3181 tcagggttga tccccagttt ttccgcacgc gcacgaacca ccgttaacgc cgcctgaata
     3241 gactcctgca tcacttcgcc gagcgaaccg gtataggtca gtttgccttt acccggaaca
     3301 catgcggttt caatggtcag caagtcaccg cccacttccg tccacgccag accggttacc
     3361 tgaccgacac ggttttcgtt atcagcgcga ccatagtcga aacgctgaac accgaggtag
     3421 tcatgcaggt tatcgccgtt aatttcgata tgttttaatg acttatcgag cagtaactgc
     3481 ttaaccgctt tgcggcacag tttggagatt tcacgctcca gaccacgcac gcccgcctca
     3541 cgggtgtagt aacgaataat gccgataatg gcgctatcgt cgacggtcag ctcacctttt
     3601 ttcagtgcat tacgttcaat ctgcttcggc agcaggtgac gtttggcgat gttcagtttt
     3661 tcatcttcgg tataaccgga gaggcgaatc acttccatac gatccagcag cggtgccgga
     3721 atgttcatgg agttcgacgt cgcgacaaac atcacgtcgc tgagatcgta atccacttcc
     3781 aggtagtggt cgctgaacgc tacgttctgc tctggatcca gcacttcaag cagtgcagag
     3841 gccggatcgc ctcgcatgtc agaagacatt ttgtcgatct catcgagcag gaacagcggg
     3901 tttttcacgc ccactttcgc cattttctgg atcagtttac ccggcataga accgatgtaa
     3961 gtacggcggt gaccacggat ttccgcttca tcacgcacgc cgcccagcgc catacggaca
     4021 tatttacgcc cggtggcttt ggcaatggac tgaccaagag aggttttacc tacccccggc
     4081 ggccctacca ggcagaggat cggtcccttg attttgttga cacggctttg aaccgcaagg
     4141 tactcaagga tgcgatcttt cacgcgctcc agaccataat ggtcggtatc aaggatttcc
     4201 tgcgcctgac gcaggtcttt tttgaccttg ctgcgcgcat tccacggtac ctgtaccatc
     4261 cagtcgatat aaccacgcac tacggtcgct tctgccgaca tcggagacat cattttcagc
     4321 ttctgcaact ctgcttccgc tttctctttt gcctctttcg gcatcttcgc cgcgtcgatt
     4381 ttgcgcttca gggcttcgtt ttcgtccggc gcgtcgtcca tttcaccgag ttctttctga
     4441 atagctttca tttgctcgtt cagatagtac tcacgctggg atttctccat ctgcttttta
     4501 acgcggttgc gaatgcgttt ctcaacctgc agcagatcga tttccgattc catcattgcc
     4561 atcagatatt ccagacgttc gttaacgtcg gacatctcca gaacggactg tttgtcagcc
     4621 agtttcagcg gcatatgtgc agcaatggta tccgccagac gcgccggatc gtcgatgcta
     4681 ttcagcgacg tcagcacttc tggtgggatt tttttgttca gcttgatgta gccttcgaac
     4741 tggctgattg cagtacgcac cagcacttcc tgttcccgct catcaatggt cggcgactcc
     4801 agatactccg ccttcgcaga aaagtgttcg ccattgtcag agagcgcaga aatacgcgcg
     4861 cgctgtaacc cctcgaccag cactttgacg gtgccgtcag gcagtttcag catctgcaat
     4921 atagaggcca cggtcccgac ggtgaaaaga tcgtttacac ccggctcatc cgttgaagct
     4981 tctttctgcg cgaccagcat aattttttta tcatggtcca tcgccgcttc cagacaacgg
     5041 atagattttt cccgcccgac aaataagggg atgaccatgt gcggataaac caccacatcg
     5101 cgcagcggca atacggggat ttcaatgcgt tcagaacgct caggattcat agagctctct
     5161 cttagtttaa tttccgccag gtaatcagat gacacgactg tgcttcacgc catctattaa
     5221 catgtacgtc agtatatggg gatgtttccc ccacattcaa cgccgagaat agaggaaaaa
     5281 ttaaagggga gataaaatcc cccctttttg gttaactaat tgtatgggaa tggttaatta
     5341 ttcaccagat gcctgttgcg cttccggctt accataaatc agcaacggtt tgctttgacc
     5401 atcaattacc gactcgtcga taaccacttt ttcgacgtct tccatggacg gcagatcgta
     5461 catggtatcg agcagtgcgg cttctacgat ggaacgcagg ccacgggcac cggttttacg
     5521 cgccatcgct ttcttagcga tagcatccag cgcctcgtca cggaattcca gatccacgcc
     5581 ttccagatta aacagcgcct gatactgctt ggtcagggcg tttttcggct ctttgaggat
     5641 ctgaatcaga gcttcttcgc tcagttcatt caacgttgct acaaccggca gacgaccaat
     5701 gaactcaggg ataagaccaa acttgatcag atcttccggt tcaacctgcg ccagcagctc
     5761 gccttcgctt gctttgtcgg acttcgcttt taccgtcgcg ccaaaaccaa tgccggagcc
     5821 ggtttctaca cggtgggaaa tcactttatc cagaccggca aacgcaccgc cacaaataaa
     5881 caggatctta gaggtatcaa cctgcaagaa ttcctgctgc ggatgtttac gcccaccttg
     5941 cggtggaaca gcagctaccg taccttcgat cagtttcaac agtgcctgct gtacgccttc
     6001 accggaaacg tctcgggtaa tggacgggtt gtctgactta cgagaaatct tgtcgatttc
     6061 atcgatgtag acaatcccgc gctgcgcttt ctgtacgtcg taatcgcact tttgcaacag
     6121 cttctgaatg atgttttcaa cgtcttcgcc cacataaccg gcttcggtca gcgtggttgc
     6181 gtcggccatg gtgaacggga cgtccagcag gcgcgccagc gtttcagcca gcagcgtttt
     6241 accggaaccg gtcggaccga tcagcagaat gttacttttg cccaactcga caccattgct
     6301 ggtatcgccg ttgcgcagac gtttgtaatg gttgtatacc gcgaccgcca gcactttttt
     6361 cgcctgttcc tggccgataa cgtaatcgtc caggtggttg cgaatttcat gcggcgtcgg
     6421 tagcgcactg cgttcacgat gcggtgcaac ttctttaatc tcttcgcgaa tgatgtcgtt
     6481 acataaatca acacattcgt cgcagatata cacggatgga ccggcaatca gcttgcgcac
     6541 ttcatgctgg cttttgccgc aaaaagagca atacagcaat ttgcctgagc catctttgcg
     6601 tttatctgtc atgggtcaaa acctcttctt tgttctttgt gccgcacacg acgacgcaaa
     6661 tgccattctc aggcgcaagc cgcttacagc gttgtgccgc cctggataag tatagcggca
     6721 cagttgcgcc tctggcatca attacgatgg gtcagaatcg aatcgaccag accgtattcc
     6781 accgcttcag gggcggaaag gaagcgatcg cgctcggtat cacgttcaat ctgttctaat
     6841 gattgacccg tatgaagcgc cataagttca ttcatgcgcc ctttaacttt cagaatttca
     6901 cgggcatgaa tttcgatatc ggtcgcctgg ccctggtagc cgcccaacgg ttggtgaatc
     6961 atcacgcgcg aattcggcag gcaaaaacgt ttaccttttg cccctgcggt cagcaagaaa
     7021 gcgcccatcg aggccgcctg gcccatacag atggtgctga catcaggctt gataaactgc
     7081 atggtgtcat agatagacat cccggcagtg atcaccccgc ctggggagtt aatgtacaga
     7141 tagatatctt tttctgggtt ttccgcttct aggaacagca tctgcgccac aatcaggttc
     7201 gccatgtggt cttcaacctg gccagtcaga aaaatgacgc gttccttaag tagacgagaa
     7261 tagatatcaa aagagcgctc accgcgtgag gtctgttcaa tgaccatcgg caccagcgcc
     7321 atatggggtg caaagttatc tcgttcgccg ctgtatgaca tttccgtctc ctggataaaa
     7381 ttgaaaaaac ctgctgtacc gattgtaacc ttatggacgg attatcagct agtccctgtg
     7441 ttatgggtac ggcatcacca tatttcaagc ataacaatct tttgttgtta cgctaacacc
     7501 gaaagaggct tttcgcacgg ttccatgcaa aattcatgac aaaaaaaagc ccaccacctg
     7561 gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg cgtaaattac gcctgctggt
     7621 tcatcagctc gttgaaagtg gtttcttttt cagtcacttt cgctttcgcc agtacagctt
     7681 caacagcctg ttcttccaga gcaacattgc gcatgttgtc catcagttct ttgtttttgc
     7741 tgtagaactc gataacttct ttcggatctt cgtacgcaga agccatctct tcgatcaggc
     7801 ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg gataacttcg cccagcagca
     7861 ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag ttcgcgcggc agttccagag
     7921 ctcgtttttc gttgccaccg aaacgctgtg cagcctggcg acgcagaacg tcgatttcgc
     7981 tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt taccagacct tcgatcgcct
     8041 gagacttaac gcggttacgg atggcgctct tcagctcgcg ctccatgttt ttacgcactt
     8101 cagcgcgcag accttctacg gaaccatctt caacgccgaa acgtttgatg aattctgcag
     8161 tcagttccgg cagttcacgc tcttcaactt tcttcaggtt gatagcgaat ttcgctgctt
     8221 tacctttcag attttctgcg tggtattctt ccgggaaggt cacgtcgatg gtgaactctt
     8281 cgccagcttt gtggcctttg ataccgtctt caaagcccgg gatcatacga ccctggccca
     8341 tcgccagtac gaaatcagac gctttaccgc cttcgaactc ttcgccgtct acagaaccgg
     8401 tgaagtcgat ggttacgcgg tcttctgctt caacggcgcc gtctttttct ttccaggtcg
     8461 cctgctgttt acgcagagta tccagcatgc cgtcaacgtc agcgtcggtc acttcaacga
     8521 tcggtttttc aacttcgatc gcttccagac cctgcagttc aacttccgga taaacttcaa
     8581 actctacaga gtaggtgaag tcttcaccca gcttgtattc gcccggaaca taagtcggtg
     8641 cgccagccgg attgattttt tctttaatga tggcgtcaat gaagttacgg ctcatcaggt
     8701 cacccagaac gtcctggcgt acagacgcgc cataacgctg agcaacgata ttcattggca
     8761 ctttgccttt gcggaagccg tcaatacgta cttttttcgc aacgttgacc agctcgcttt
     8821 taacagcggt ctcgatgctg tcagcagcga tagtaatcgt tacacggcgg ccaaggcctt
     8881 gagtggtttc aactgaaact tgcatcttgt tacctcaaaa aatcacagtg ctcggtcaac
     8941 tcttaccccg caagcacagg ttgttggctt cgcggaaccg ggatgttctt ataatcaatc
     9001 acattccctg ttaccagaat catcccgaaa gcattcaaat aggacgcggc attatagcgg
     9061 catcactttt atgagtcgag aacggttatt gcgcgctgtc gcgtattttt tcacaattcc
     9121 cgggcaattt tcgtctaaat gctgggcaaa aagcgcaaaa tccagacaaa acaaaacggc
     9181 ccgcaggccg tttttcataa aatctttagc aacatactgg aaaagcgccg acagttgcaa
     9241 atgcgttttt acgcgatgct tcctgctcca cgacagggag gagaggcaaa gacggtgtcc
     9301 tgcaaccctt cccactcctt aatagtgtaa gtatgcagag ccagcgcatg aacggtagta
     9361 gagagttcct ccgctaaagt actgtaaatc attcgatgac gattcagaaa acgttcaccc
     9421 gtaaaacgat cgctgaccag cacaacttta aaatggcttt cagagccggc tgggacattg
     9481 tgacgatagc tttcatccac tacttcgagg aatacgggtt ggaacgccgc ccttaatttt
     9541 tcttctatcc gctcacgtat catcatgaaa tatctcctcc gacaacgctg agatgtcatc
     9601 catcccttta aatactagcc gcttttaccg tttccattgc agcttaacaa caaatattta
     9661 ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac tgatttaaag attgaaacac
     9721 aggcctcgca tcagcgtttc ttttaccagg ggtgattatt tcgccaacat gatgaattta
     9781 catgccttac ccacttcccc tcgccgttgg caatgttatg atggcgggaa tttttaccaa
     9841 acacgcttaa gagtctctga gagcccgaac atgttcaaaa aaatcctctt cccgttagtt
     9901 gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa ctattgaagt ttccccgacg
     9961 attacactgc cacagcagga tccaagcctg atgggcgtca ccgtaagcat taatggtgcc
    10021 gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg ataatcaaat cgttaccctg
    10081 accgcctccc gagatctgcg tttcctgctg caagaagtgc tggaaaaaca gatgaccgcg
    10141 cgtggttaca tggttggtcc gaatggcccg gttaatctgc aaatcatcgt tagccaactg
    10201 tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg cgaccaaagc ggatatcgcc
    10261 atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa actatcgtgc cagctacaac
    10321 gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg atgcggttaa cagtgtgctg
    10381 accgatacca tcgctgatat gtctcaggac accagcatcc acgaattcat caagcagaac
    10441 gcgcgttaat ttctgccctc tggcccggtg caaaccgggc ctgtagacgc ccatgtccag
    10501 tcaatattta cgtatttttc aacagccgcg ttcagccata ttgctgatcc tgggttttgc
    10561 ttccgggcta ccgctcgccc tgacatccgg taccttacag gcctggatga cggtcgagaa
    10621 tattgatctc aaaaccattg gtttcttctc tctggtaggc caggcttacg tttttaaatt
    10681 cctctggtca ccgctgatgg accgctacac gcccccattt tttgggcggc ggcgcggttg
    10741 gctgctcgcc acgcaaatcc tgttattagt cgccattgcg gcgatgggtt ttctcgaacc
    10801 tggcacccaa ctccgctgga tggcggcact ggcggtggtg atcgcttttt gctcggcctc
    10861 ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc ccggcagaag aacgtggtgc
    10921 gggcgcggca atcagcgtgc tgggttaccg tttagggatg ctggtttccg gcggcttagc
    10981 cctgtggctg gcagataaat ggctgggctg gcagggcatg tactggttga tggcggcgct
    11041 gttgatccct tgtattatcg cgacgttgct tgcaccagaa ccgaccgaca ccattcctgt
    11101 gccaaaaacg ctggaacaag cggttgttgc acctctgcga gatttctttg gtcgcaataa
    11161 tgcctggctt attttgcttc ttatcgtgct gtataagctg ggcgacgcat tcgccatgag
    11221 cctgacaacc acgtttttga ttcgcggcgt cgggtttgat gcgggtgaag taggcgtggt
    11281 taacaaaacg cttggcttac tggcgaccat tgtgggcgca ttgtacggtg ggattttgat
    11341 gcagcgcctg tcgctgttcc gggcgctgct gattttcggc attttacaag gtgcgtctaa
    11401 cgctggttac tggctgctgt cgattactga taagcatctc tacagcatgg gcgcagccgt
    11461 ctttttcgaa aacctctgtg gcgggatggg cacatcagcc tttgtcgcgc tgttaatgac
    11521 gctgtgtaat aagtcatttt ccgctactca atttgccctg ctctcagcgc tttccgctgt
    11581 aggacgagtt tatgtcggcc ccgtggcggg ttggtttgtt gaagcacacg gctggtcgac
    11641 attctatcta ttctccgtcg ccgctgccgt accggggctt attttgctgc tggtttgccg
    11701 ccagacgctt gaatatacac gagtaaatga caactttatc tcccgtaccg aatatccgac
    11761 aggttatgcc tttgccatgt ggacactggc ggcgggcgtc agcctgttgg ccgtgtggtt
    11821 actgctgttg acgatggacg cgctggattt gacgcacttc tctttcctgc ctgctctgct
    11881 ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt ggtggtttgc tggattatct
    11941 ggctctacga aaaacgcatc tgacgtaatc tgtaaatatt atttagctgt agtaatcact
    12001 cgccgtaatt attacggcta aataaacatc agtatcgctt aataatattt gttcttttgt
    12061 gcggcttagc gtttggaaat tattgacgca atttatgaat tctatttttt cttacacgat
    12121 tcagctaatg agtctttatt ttccccccac ccaattgtca ccataatgat aattatttgt
    12181 taaacaattg ttttattcca gattggttat accaattgcc cgtccaaact ccttctgcac
    12241 tcccctttcg ttataacgcc cttttgcaac agcttcttaa aatcaacttg atatgttttg
    12301 caacatatgt aacctgacag ccaaatccaa gtaacaggaa tttaatcatg cttacagtaa
    12361 tgtaacctcc ccgtaaaatg cccacacact ttaaacgcca ccagatcccg tggaattgag
    12421 gtcgttaaat gagactcagg aaatacaata aaagtttggg atggttgtca ttatttgcag
    12481 gcactgtatt gctcagtggc tgtaattctg cgctgttaga tcccaaagga cagattggtc
    12541 tggagcaacg ttcactgata ctgacggcat ttggcctgat gttgattgtc gttattcccg
    12601 caatcttgat ggctgttggt ttcgcctgga agtatcgtgc gagcaataaa gatgctaagt
    12661 acagcccgaa ctggtcacac tccaataaag tggaagctgt ggtctggacg gtacctatct
    12721 taatcatcat cttccttgcg gtactgacct ggaaaaccac tcacgctctt gagcctagca
    12781 agccgctggc acacgacgag aagcccatta ccatcgaagt ggtttccatg gactggaaat
    12841 ggttcttcat ctacccggaa cagggcattg caaccgtgaa tgaaatcgct ttcccggcga
    12901 acactccggt gtacttcaaa gtgacctcca actccgtgat gaactccttc ttcattccgc
    12961 gtctgggtag ccagatttat gccatggccg gtatgcagac tcgcctgcat ctgatcgcca
    13021 acgaacccgg tacttatgac ggtatctccg ccagctacag cgggccgggc ttctcaggca
    13081 tgaagttcaa agctattgca acaccggatc gcgccgaatt cgaccaatgg gtcgcaaaag
    13141 cgaaacagtc gccgaacacc atgtctgaca tggctgcgtt cgaaaaactg gccgcgccta
    13201 gcgaatacaa ccaggtggaa tatttctcca acgtgaaacc agacttgttt gccgatgtga
    13261 ttaacaagtt tatggctcac ggtaagagca tggacatgac ccagccagaa ggtgagcaca
    13321 gtgcacacga aggtatggaa ggcatggaca tgagccacgc ggaatccgcc cattaaaggg
    13381 gttgaggaag aataaagatg ttcggaaaat tatcacttga tgcagtcccg ttccatgaac
    13441 ctatcgtcat ggttacgatc gctggcatta ttttgggagg tctggcgctc gttggcctga
    13501 tcacttactt cggtaagtgg acctacctgt ggaaagagtg gctgacctcc gtcgaccata
    13561 aacgcctcgg tatcatgtat atcatcgtgg cgattgtgat gttgctgcgt ggttttgctg
    13621 acgccattat gatgcgtagc cagcaggctc ttgcctcggc gggcgaagcg ggcttcctgc
    13681 cacctcacca ctacgatcag atcttcaccg cgcacggcgt gattatgatc ttcttcgtgg
    13741 cgatgccttt cgttatcggt ctgatgaacc tggtggttcc gctgcagatc ggcgcgcgtg
    13801 acgttgcgtt cccgttcctc aacaacttaa gcttctggtt tactgttgtt ggtgtgattc
    13861 tggttaacgt ttctctcggc gtgggcgaat ttgcgcagac cggctggctg gcctatccac
    13921 cgctatcggg aatagagtac agtccgggag tcggtgtcga ttactggata tggagtctcc
    13981 agctatccgg tataggtacg acgcttaccg gtatcaactt cttcgttacc attctgaaga
    14041 tgcgcgcacc gggcatgacc atgttcaaga tgccagtatt tacctgggca tcactgtgcg
    14101 cgaacgtact gattattgct tccttcccaa ttctgacggt taccgtcgcg ttgttgaccc
    14161 tggatcgcta tctgggcacc catttcttta ccaacgatat gggtggcaac atgatgatgt
    14221 acatcaacct gatttgggcc tggggccacc cggaagttta catcctgatc ctgcctgttt
    14281 tcggtgtgtt ctccgaaatt gcggcaacct tctcgcgtaa acgtctgttt ggttatacct
    14341 cgctggtatg ggcaaccgtc tgtatcaccg tgctgtcgtt catcgtttgg ctgcaccact
    14401 tctttacgat gggtgcgggc gcgaacgtaa acgccttctt tggtatcacc acaatgatta
    14461 tcgccatccc gaccggggtg aagatcttca actggctgtt caccatgtat cagggccgca
    14521 tcgtgttcca ttctgcgatg ctgtggacca tcggttttat cgtcactttc tcggtgggcg
    14581 ggatgactgg cgtgctgctg gcagtaccgg gcgcggactt cgttctgcat aacagcctgt
    14641 tcctgattgc gcacttccat aacgttatca tcggcggcgt ggtcttcggc tgcttcgcag
    14701 ggatgaccta ctggtggcct aaagcgttcg gtttcaaact gaatgaaacc tggggtaaac
    14761 gcgcgttctg gttctggatc atcggcttct tcgttgcctt tatgccgctg tatgcgctgg
    14821 gcttcatggg catgacccgt cgtttgagcc agcagattga cccgcagttc cacaccatgc
    14881 tgatgattgc agctagcggt gcagtactga ttgcgctggg tattctctgc ctcgttattc
    14941 agatgtacgt ttctattcgc gaccgcgacc agaaccgtga cctgactggc gacccgtggg
    15001 gtggccgtac gctggagtgg gcaacctctt ccccgcctcc gttctataac tttgccgttg
    15061 tgccgcacgt tcacgagcgt gatgcattct gggaaatgaa agagaaaggc gaagcgtata
    15121 aaaagcctga ccactatgaa gaaattcata tgccgaaaaa cagcggtgca ggtatcgtca
    15181 ttgcagcttt ctccaccatc ttcggtttcg ccatgatctg gcatatctgg tggctggcga
    15241 ttgttggctt cgcaggcatg atcatcacct ggatcgtgaa aagcttcgac gaggacgtgg
    15301 attactacgt gccggtggca gaaatcgaaa aactggaaaa ccagcatttc gatgagatta
    15361 ctaaggcagg gctgaaaaat ggcaactgat actttgacgc acgcgactgc ccacgcgcac
    15421 gaacacgggc accacgatgc aggcggaacc aaaatcttcg gattttggat ctacctgatg
    15481 agcgactgca ttctgttctc aatcttgttt gctacctatg ccgttctggt gaacggcacc
    15541 gcaggcggcc cgacaggtaa ggacattttc gaactgccgt tcgttctggt tgaaactttc
    15601 ttgctgttgt tcagctccat cacctacggc atggcggcta tcgccatgta caaaaacaac
    15661 aaaagccagg ttatctcctg gctggcgttg acctggttgt ttggtgccgg atttatcggg
    15721 atggaaatct acgaattcca tcacctgatt gttaacggca tgggtccgga tcgcagcggc
    15781 ttcctgtcag cgttctttgc gctggtcggc acgcacggtc tgcacgtcac ttccggtctt
    15841 atctggatgg cggtgctgat ggtacaaatc gctcgtcgcg gcctgaccag cactaaccgt
    15901 acccgcatca tgtgcctgag cctgttctgg cacttcctgg atgtggtttg gatctgtgtg
    15961 ttcactgttg tttatctgat gggggcgatg taatgagtca ttctaccgat cacagcggcg
    16021 cgtcccatgg cagcgtaaaa acctacatga caggctttat cctgtcgatc attctgacgg
    16081 tgattccgtt ctggatggtg atgacaggag ctgcctctcc ggccgtaatt ctgggaacaa
    16141 tcctggcaat ggcagtggta caggttctgg tgcatctggt gtgcttcctg cacatgaata
    16201 ccaaatcaga tgaaggctgg aacatgacgg cgtttgtctt caccgtgcta atcatcgcta
    16261 tcctggttgt aggctccatc tggattatgt ggaacctcaa ctacaacatg atgatgcact
    16321 aagagcggcg gttatgatgt ttaagcaata cctgcaagta acgaaaccag gcatcatctt
    16381 tggcaacctg atctcggtga ttgggggatt cctgctggcc tcaaagggca gcattgatta
    16441 tcccctgttt atctacacgc tggttggggt gtcactggtt gtggcgtcgg gttgtgtgtt
    16501 taacaactac atcgacaggg atatcgacag aaagatggaa aggacgaaga atcgggtgct
    16561 ggttaaaggc ctgatctctc ctgctgtctc gctggtgtac gccaccttgc taggctttgc
    16621 tggctttatg ctgctgtggt ttggcgcgaa tccgctggcc tgctggctgg gggtgatggg
    16681 ctttgtggtt tatgtcggcg tttatagcct gtacatgaaa cgccactcgg tctacggcac
    16741 attgattggt tcgctctccg gcgctgcgcc gccggtgatt ggctactgtg cagtaaccgg
    16801 cgagttcgat agcggcgcag cgatcctgct ggctatcttc agcctgtggc agatgcctca
    16861 ctcctatgcc atcgccattt tccgctttaa ggattatcag gcggcaaaca ttccggtatt
    16921 gccagtggta aaaggcattt cggtggcgaa gaatcacatc acgctgtata tcatcgcctt
    16981 tgccgttgcc acgctgatgc tctctcttgg cggttacgct gggtataaat atctggtggt
    17041 cgccgcggcg gtgagcgtct ggtggttagg tatggccctg cgcggttata aagttgctga
    17101 tgacaggatc tgggcgcgca agctgttcgg cttctctatc atcgccatca cagccctctc
    17161 ggtgatgatg tccgttgatt ttatggtgcc ggactcgcat acgctgctgg ctgctgtttg
    17221 gtaacacaac ctctctatta aaaaggtgct acggcacctt ttttattgtc tgagatcaga
    17281 gatacggcta ataaccgtat gtcttgacag agtgagcaga tgaacataaa ttaatcatac
    17341 ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag ttaatcaaag
    17401 aggtcataat gcctggaaaa acagccactc tacccgatgt cgataaaacg ctgaaaaatg
    17461 cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa gctgcgcaag
    17521 ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc gccgaaagtg
    17581 tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg atgaaccaac
    17641 tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa aggaaaaaca
    17701 gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg taattatcag
    17761 tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaactctgt tatcgataaa
    17821 tttgtacgcg catcgctaaa gaaaagtgtg cgtaatagcg actgtgcggc taaagcactt
    17881 attgacagac aaagtggtga actgatcggc atctgtactt ttacggcata ttcgctggaa
    17941 aaacaacgcg tttctggcgt ccttcagggt tcacaacctt cagaaattgg tgttgtcaga
    18001 ttagtcatgt tgggggtagc acggaagtat caaaagcggg gctttggtca ggacctacta
    18061 tgtgattttt ttgaacatgt aaaaataatt caccaggcat taccaattaa aggggtttat
    18121 cttgatgctg accctgccgc cattaatttt tatgctcgtc tcggctttgt tcagctttca
    18181 gcgacaccaa atgcttttgg tgctgtacct atgtttttgg cgattcagca tattctcgcg
    18241 gcttagatta ttatcgccac ccgccagggc aagttttgcc ttagccgggg gctgagcagc
    18301 gttaccactt ttgttcaatt gcccccgctt tcagcaactc cacatccttg ccgttaatgg
    18361 ttgccgttgc ggcaacgatt gtggtcctta cagttgcaca agattttcgc tcaaattccg
    18421 gtttataaag aataagagac gaattactgg taaatgaacc aaataggtaa tcagtaaagc
    18481 taatattcag ccggtttaaa ccgaaaggcg cgaagccaac cgtttggtta atctccttac
    18541 cggatgggcg acgaatgctg aatgtcacgc gaagattggc cacttcgcct aatttattgg
    18601 tggtggcgaa gaggctatcc atgcaggaaa aacctttatc taaaaaatta tctcgcatcc
    18661 cagaacgggt aaccgtaatc acgggtaacg tatcgtcaac cgtttttctg tcaatcatgt
    18721 cttttcgaca agtagccaga aagtcttccg ggccaccata aatgtggaca cctttatcaa
    18781 gatattcctt cgctttctta caatctacct caacatactg accattggcg tacataatgc
    18841 cc
//
LOCUS       DAZPEP010000073        18517 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZPEP010000073 DAZPEP010000000
VERSION     DAZPEP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18517
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.073"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            78..290
                     /gene="cspH"
                     /locus_tag="ACHUT9_002463"
     CDS             78..290
                     /gene="cspH"
                     /locus_tag="ACHUT9_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFR1348911.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            737..1042
                     /locus_tag="ACHUT9_002464"
     CDS             737..1042
                     /locus_tag="ACHUT9_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348912.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1149..1793
                     /gene="gfcB"
                     /locus_tag="ACHUT9_002465"
     CDS             1149..1793
                     /gene="gfcB"
                     /locus_tag="ACHUT9_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFR1348913.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1790..2536
                     /locus_tag="ACHUT9_002466"
     CDS             1790..2536
                     /locus_tag="ACHUT9_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFR1348914.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2536..4632
                     /locus_tag="ACHUT9_002467"
     CDS             2536..4632
                     /locus_tag="ACHUT9_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFR1348915.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSQLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4678..5817
                     /locus_tag="ACHUT9_002468"
     CDS             4678..5817
                     /locus_tag="ACHUT9_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFR1348916.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5805..6251
                     /gene="etp"
                     /locus_tag="ACHUT9_002469"
                     /gene_synonym="yccY"
     CDS             5805..6251
                     /gene="etp"
                     /locus_tag="ACHUT9_002469"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFR1348917.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6271..8451
                     /gene="etk"
                     /locus_tag="ACHUT9_002470"
     CDS             6271..8451
                     /gene="etk"
                     /locus_tag="ACHUT9_002470"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFR1348918.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8566..9864)
                     /gene="appA"
                     /locus_tag="ACHUT9_002471"
     CDS             complement(8566..9864)
                     /gene="appA"
                     /locus_tag="ACHUT9_002471"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFR1348919.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="ACHUT9_002472"
     CDS             complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="ACHUT9_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFR1348920.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10049..11185)
                     /gene="appB"
                     /locus_tag="ACHUT9_002473"
     CDS             complement(10049..11185)
                     /gene="appB"
                     /locus_tag="ACHUT9_002473"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1348921.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11197..12741)
                     /gene="appC"
                     /locus_tag="ACHUT9_002474"
     CDS             complement(11197..12741)
                     /gene="appC"
                     /locus_tag="ACHUT9_002474"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFR1348922.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12875..13732)
                     /locus_tag="ACHUT9_002475"
     CDS             complement(12875..13732)
                     /locus_tag="ACHUT9_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFR1348923.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="ACHUT9_002476"
     CDS             complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="ACHUT9_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFR1348924.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14124..14711)
                     /gene="hyaD"
                     /locus_tag="ACHUT9_002477"
     CDS             complement(14124..14711)
                     /gene="hyaD"
                     /locus_tag="ACHUT9_002477"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFR1348925.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGRLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="ACHUT9_002478"
                     /pseudo
     CDS             complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="ACHUT9_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="ACHUT9_002479"
     CDS             complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="ACHUT9_002479"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFR1348926.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="ACHUT9_002480"
     CDS             complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="ACHUT9_002480"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFR1348927.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4545 a         4916 c         4784 g         4272 t
ORIGIN      
        1 cggcggtctt aaatatcgct ggctttattt tattaacccc gtatagtgca gcgcagtatt
       61 ttcagtaagg aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc tttgatcgca
      121 aaagcggcaa aggattcatt atcccctccg acggtcgtaa agaagttcag gtccatattt
      181 ccgcattcac tccccgcgac gcagaagtgc ttataccagg attacgtgtg gaattttgcc
      241 gcgtaaatgg cctgcgagga ccaacagcgg caaatgttta tctctcttaa tatgacgctg
      301 cttgtttaaa gctgactggt tttattggaa tagtgcaata gtcaacatca tacattataa
      361 catcagttac ttttccacaa agaaactaat ccatttaagc gaatatctct gcacctaata
      421 gttataaatg ccatttaaca gccagttata gtaccgcttg aaactatatt accaacaata
      481 attaatagct ttcgtaacaa tttccttatc tttaatttgt attttgctaa gatttatctt
      541 atgctcacaa gtcgtatttc cagaggggat cgttaataat cttatatttc cctctgcgca
      601 cctttcctac ccgcagtatc agtatatagt gcaagcggta tgaggaaagg gagatttcac
      661 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
      721 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
      781 gacgccagct gcgtttgctg cctctgaagc aacaaacggg accgaggcaa cgacgggtac
      841 tactggcacc acaacgacca ctaccggcgc aaccacgact gctactacca ctggtggcgt
      901 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc
      961 gacgttggca gttgttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
     1021 caccacgtcc actacacgct aatactgctt taacgcgtcg aaattaaagt taaatgatac
     1081 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
     1141 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
     1201 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
     1261 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
     1321 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
     1381 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaaaacc gtcaaactta
     1441 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
     1501 taaaagatgg cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
     1561 ctgcgaccct gagttctact ttttcctttg ctggattaga gacgctgaat attgcgggtc
     1621 gcaatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgcggcca gaaaaacagt
     1681 ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt
     1741 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
     1801 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
     1861 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccatggaaaa
     1921 tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt
     1981 gctgaccgac tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatggcaca
     2041 gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
     2101 ccagcagtta ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt
     2161 tgtacgagtg gatgagaaca gcaatccccc gttggtcggg gactatacgc tctataccgt
     2221 acagcgtccg gtgactatta ctctgctggg tgcggtttct ggggccgggc aattgccctg
     2281 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgccttg caggcgcgga
     2341 caagaataac gttatggtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
     2401 gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttgg gcttttctgc
     2461 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
     2521 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc
     2581 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca
     2641 gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa
     2701 ttcagcgtca actaccggga taacgatcaa taccggttct actccacctc cgtggcgctg
     2761 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgcaa atatagccag
     2821 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
     2881 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt
     2941 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
     3001 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
     3061 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
     3121 agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg
     3181 aatcccctgc gtctgaaact cgaatacgat ggcaacaatt accagaatga tttcgctggc
     3241 aaactgcctc aggcaagcca tttcaacgtc ggcgcagttt atcgcgctgc cagctgggca
     3301 gatctcaacc tgagttatga acgcggtaac acgttgatgt ttggcttcac gttacggacc
     3361 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
     3421 cctgaatctg aaggattgca gtacaccaca gtagcgaacc aacttaccgc gctgaagtac
     3481 aacgcaggtt ttgaagcacc ggaaattcag ctgcgcgata agacgctgta tatgtctggt
     3541 caacaataca aataccgtga ttctcgcgaa gcggtcgatc gtgccaaccg gattctggtg
     3601 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
     3661 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggctgg tacagcgcct
     3721 ggtcaatcag agcaacttca acaacaacgt gttgaagcag aagatctttc tgcctttggt
     3781 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
     3841 tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagtgcccgc
     3901 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
     3961 tacgacaagt ttaagtcttc gcagttgccc gcggactcta ccctgccccg cgtgcgcacg
     4021 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt
     4081 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtac
     4141 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
     4201 gacgttaact acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc
     4261 acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
     4321 aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa
     4381 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
     4441 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
     4501 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
     4561 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
     4621 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
     4681 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
     4741 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
     4801 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
     4861 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgca
     4921 aactatgaat atcgtatcgg gattggtgat gtgttgatgg ttacggtctg ggatcacccg
     4981 gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac
     5041 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt
     5101 acccaggtac gcaacgagat tacagcgcgt ctcgattcag tcattgaaag cccgcaagtc
     5161 gatgtcagcg tcgcggcgtt ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa
     5221 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
     5281 ggcggtctga ccgcagatgc agactggcgc aacgtggtgc tcacccagaa tggcgtaaag
     5341 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
     5401 cacccgggcg atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
     5461 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
     5521 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
     5581 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
     5641 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
     5701 gtcactaccg cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt
     5761 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
     5821 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
     5881 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctgataa
     5941 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
     6001 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
     6061 tggagtcgga acatattgct caggttaccg caattgctcc cgaggtccgc gggaagacaa
     6121 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
     6181 acgcatttga acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
     6241 tcagccgata atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
     6301 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
     6361 cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg
     6421 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
     6481 gccattctca gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag
     6541 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
     6601 gacatagttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
     6661 gaaaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
     6721 cagcaactga cactcacggt tggggaaaac ggccactata cactggaagg tgaagagttc
     6781 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
     6841 gacattaagg ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg
     6901 attaacgcat tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
     6961 gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
     7021 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
     7081 ttcttacagc gccagttacc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc
     7141 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
     7201 gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc
     7261 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
     7321 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg caatgccttc cacccaacag
     7381 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
     7441 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc
     7501 gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt
     7561 ggttttattc ttggcctgtt tatttctgtg ggtgccgtgc tggcgcgtgc gatgttgcgt
     7621 cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta tgccactatc
     7681 ccaatgtccg agtggctgga taaacgcacc cgtctgcgta agaaaaattt attttctaat
     7741 cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct
     7801 gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagacggag
     7861 aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca
     7921 actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta
     7981 cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat
     8041 ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat
     8101 gtgattactc gcggtcaggt gccacctaac ccgtctgaac tgctgatgcg cgatcggatg
     8161 cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgtcga tacgccgccg
     8221 atgctggcgg tgagtgatgc cgcggtcgtg gggcgttctg ttggcaccag cctgctggtt
     8281 gcgcgttttg gcttgaacac cgccaaagag gtgagtttgt caatgcagcg tctggaacag
     8341 gcaggcgtca atattaaagg cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct
     8401 tacagttacg gctataacta ttacggttat agttactccg agaaagagta aaactgaaag
     8461 tggccggatg cggcgtgaac gccttttccg gtctacgcat tagacagttc ttcgtttgtc
     8521 atcagcctca gagcattcag gtaactgaat gctctttttt atgcattaca aactgcacgc
     8581 cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc aacgaacaca tgccctgcgc
     8641 atttcgctct tcacatcctg ccagggtcag tttcacctct ccgggcggcg tatttaatga
     8701 cagcggcgtt ttatcacgca tctgctgtaa agtctggaag accagcgaaa cctgaatcca
     8761 ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc agttcaccac ctggcggcgt
     8821 gttatccggc tgaccgggaa gcgtccagtt gagctccagt gcgccgccga gatttgccag
     8881 attagtatcg tgtccggcga taaacagcac tgaagtgggt aatgtcacac catacgcctg
     8941 tttttgcggt ggatggggcg tcaacgctgt cttgatcaaa tctaataacg gggtggcgcg
     9001 gctgcgggca acctctggcg tgcgttgtag caaataaaat tgcgcgttat gcaaacttag
     9061 caaggtgttc cactggtgtg aatcggtgat ccttccccac cccggctccg gcattccctg
     9121 tgcttgttgc aggagaaata tctccgtcag cattgatgcg aggcttaccg caccggttaa
     9181 tgagacattg tcggcgctca ccttgagttc cgatggtaat gcctgcgtta atgaacagct
     9241 ttcgtcctgt ttctcacgtt taaggcacaa gtttgattgc ggaaaattaa gcacccgttc
     9301 cagttcgcga aacgccgttt gccgatgccc ggtaaagtca gcaattgacc ctcctgccct
     9361 gctgaggatc gcgtcagtca cgttcgcgtt atccagttgg caaacgccag tttttagagg
     9421 attaaataac ggatcgggac tggacgtatc agcctgggta tgtacggtta ttgcacagtc
     9481 aggtgccagc ccggcggcga aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc
     9541 aataatcgcg acctgaccag actgcgggca gccctttttc gccagcaatc cgtcggctac
     9601 cagacgctgg cgttggtaat gtccgagata ggcgattagc tcaccaccgc gcggtgtcag
     9661 ccaacccagt tttaccggcc aggttggcca tgcgtctggg gtgacatcct gcatcagttg
     9721 cgtggccttg gttggagcac gcacaccatg acgactgaca atcaccacac tttccagctt
     9781 cagctccggc tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa
     9841 aaatgggatt aagatcgctt tcatcgatat gtttccgctt ttcatatctg acattattga
     9901 ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt tcgttaactt ttcagacgcg
     9961 ggtccagcca taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc
    10021 aaagtaaata ccacattgtt cctgctcctt agtacaactc gttttcgtta cggcggagag
    10081 tttctgttgt catgcgtccc cacattttgt agtagctcca gagagtgtag agcaacacaa
    10141 tgggcaaaaa tatcagcacg attaccaaca taatgctcag cgtcagctga ctggaagtgc
    10201 tgtcccacaa cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca
    10261 gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa tgacgccatt aaaaatcccc
    10321 aacccgggcg accacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca
    10381 gcgggaagat ccacaaaacg ggcgattcga cgaaattatt catccaggca ccaggtagca
    10441 ctgccaccag tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa
    10501 agccatcaat accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca
    10561 gtgcagcgcg tttggtggcc agctgtgaac gcagatgaat cacaccaaca gttttcagtt
    10621 gtaaccagac gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag
    10681 ggaatggcgt cagcagttgc cagaagctac cgagatactc cacgcgtaat tgcggtgtga
    10741 aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca
    10801 ccagactgcc gatgaccaga ccggcgtccc acattttacg ccagcgtgca tcggcgattt
    10861 ttccgcgata atcaaaggcc agcgggcgga agaacagtga gcacagcacc aggatcatcg
    10921 ccacataaaa gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc
    10981 caccagcgag gatcaaccag acctggttgc cttcccagtg tgcaccaacg ctgtttatca
    11041 ctatccggcg ttcatcatca ttacgcgcca ccagcggcag cagacagccg atccccatgt
    11101 caaatccgtc ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga
    11161 agcgcaatgt ttcataatca aacatgattt tcccctttac ccctgttgct gcgtcggttg
    11221 ttcactctgc atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc
    11281 gattaagaac agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt
    11341 ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg
    11401 accaaactcg gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca
    11461 gagcgccatt ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag
    11521 cgcaatcagc atcaccagta gcggcaggga accacagccc accatgatgc ggaaactcca
    11581 gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt
    11641 gacatgattc atatccggcg catagcggga gagcaacatg ccgtagccca ggtcaccttc
    11701 cagttcccgg aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat
    11761 taacagccag gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattttt
    11821 cagacccggc acggttttat ctaatgagtg agtggcgagg atccctaaca gcgcgggaat
    11881 tttgatggca aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg
    11941 tgcaggagca ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac
    12001 ttgcgcgact tcatacgcag aactgtctcc gagttgtagc gtgccgataa tcgccagagt
    12061 accgaagacg gaaccgatgg caaacgagcg taatgcaaca tcgcgctccc gtccgcgcag
    12121 taaataccag gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac
    12181 ggtgtgcaca aatttcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat
    12241 ctccatacgt agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt
    12301 ggcattcaat atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag
    12361 caggtgctgg tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga
    12421 ttcgaggaag aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac
    12481 atagttggaa tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc
    12541 cacgccaaga gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta
    12601 gattgttttg ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc
    12661 cagggtaagg ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga
    12721 taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgata
    12781 atgttagatc tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat
    12841 attcataaga ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc
    12901 actgacatac ctcgctaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca
    12961 ccacttccgg tattgggcag atttcataac tttccagtaa aggacctttt aaggtgtccg
    13021 tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc
    13081 caatggtacg aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag
    13141 cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta
    13201 catgcgccag taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg
    13261 gtaacagcga atctgtcggc aagagatttt gcgtggccgc ctgccacaac gccagcggcg
    13321 cgcagccggc ctccagtttg tcctccagca acttttcgcc gcgacgtttg cgcacccgcc
    13381 ataatccgca gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc
    13441 gcactgacac ttccccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga
    13501 gatttaacag atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat
    13561 gttgtaactc gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg
    13621 ccatactcgg ttcatcattc acctgacagc tgatcggcag tggattcatg ctgaaactgt
    13681 catcgttcgg ttgcgttcct ggccccagca gatggaaaaa agtttcgctc atgaggcacg
    13741 ctcctgttgt ggttcgacaa gcccacgcat caggtttatc agttccgctc acgggtgaat
    13801 accattcagc acaccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa
    13861 gacgccaaag cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca
    13921 tgtgtagccg ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc
    13981 tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca
    14041 gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa
    14101 cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc
    14161 aagcgaacgc cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca
    14221 ttagccggtt gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct
    14281 gcgggcagtt gctcccgtgc cagttcgctc aggcggccgc cgtagtcgtc gagcattgcg
    14341 ggttgcagac cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc
    14401 agcacttcgg agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga
    14461 atacgttctc cggcataggt tcgcagcgtt ccaggttcca gcccgtagtc aatggcatcg
    14521 agaatcaaca gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg
    14581 ccatcgacaa tctccacata ctcgggccag tggtaatggg catacaaccg ttccgccacc
    14641 cgcacgccga aaccttcatc ggcccacagc aggttgccca gccccattac cacaacgcgt
    14701 tgctcgctca tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt
    14761 tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga
    14821 catgaccgat cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt
    14881 ccattgagtt gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa
    14941 tagcgtactg gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca
    15001 tcaggaaata gccgaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg
    15061 gacgttttgc cagaacagat accagcggat ttcataccac acgccctgcc accagctttt
    15121 acgccatacc ggcacgataa acagctcgcg ggagtatcga ttgccaacaa aagcccagta
    15181 gatccgcatc agcaagacca cggtaaaaac catcccggcg ctgaagtgaa ttaacctgat
    15241 gtagcccata tagaacagat acgtcgcctc gccgctgacg gaaggtagcg gcttgccgat
    15301 aaagtatccg gtgaccatca acaccgccat gcataacacc gtcagccagt gccagatgcg
    15361 gactggcgct tcaaagacat agtggctgac aacgttgtcg cttttctgtt gcatgatgat
    15421 tctccttcgc tgttaacgca cctgcacgga gatcagctcg ctaccgtcgt cgcccagcac
    15481 gtgtgttgaa caggcgaggc acgggtcaaa gctgtgcaga gtacgcagga tctccagcgg
    15541 ttgctcgggg atcgccattt tggtgttcat cagcgccgct tcataagcgc caatctgccc
    15601 tttgggatcg cgcgggctgg cgttccaggt ggtcggcacc acgcactggt agagatcaat
    15661 cttgccatcg cgaatggcgg cccagtggcc taacgccccg cgcggcgctt cggtaaagcc
    15721 gacgccacgg cactctgtcg gccaggttgc aggttcccat ttttccgtgg aggcggtggc
    15781 aagattgccg tttttcaggt tagtcatcag cttgttgaag aaatattgca acttacctgc
    15841 ggcccactgt gcttcgtgcg cgcggcacaa aatgcggccc aacgttgact ggataccgga
    15901 aagcggcagg ttcagcgccg acatcatgcg atcgaccgac tcaacggtcg cagcatcgcc
    15961 tttgtgataa gcgattaacg tgcgtgccag cggccccact tccatcgcgt taccgcgcca
    16021 gcgcggcgct ttgatccacg agtagcgttc ctgttcattc agctgctgaa tgttggtatc
    16081 gctgcctttg acatcgccgg ggttgtacca cgggtcggta atgccatcga atggatgacg
    16141 cccgacctga tcgttgggat aacgatacca ggcgtggtcg acaaactcct gcacctgctg
    16201 cggatcaacc aaatccactg gcagcacatt gttgaagtcg ccgttaatca ccgcgccgcc
    16261 aggcatcagc agacttttct caccaaagtc gttggcaata tccgggaacg caccgtagct
    16321 gaggacacat ttatcagaaa gacccgtgcc gatttcgctc cacggtttgt tgaactgacc
    16381 gatggctaag gcgtcgggga tcatcacgtt gttaatgaag tccgccgtgc gggtgataat
    16441 cgactgcacc aggttcaggc gttccatatt gaccgccccg accgcgccgc tttcgtcaat
    16501 gttaatggcg caaggcatcc cgccgacaat ccagtttgga tgcgggtttt taccgccaaa
    16561 gaccgcgtgg attttgacaa tttcacgctg gaaatcgaga gcttcgagat agtgggcaaa
    16621 gcccatcagg ttagcttctg gcggcagttt gtactgcggg tgcccccagt agccattgcg
    16681 gaagatcccc aactgcccgc cttcaacaaa ttttttcagg cggttttgta cgtcgaagaa
    16741 atagccaggg gatgatttcg gccaagagga gagactttgc gccagttcgg aggttttccg
    16801 cgggtcggct ttcagcgcat ctaacacatc gatccagtcc atcccggcaa gctgatagaa
    16861 gtgcaccaga tgatcgtggc accagagcgt tgccagcata atgttgcgga tgatattggc
    16921 gttgtccggc actttaatac cgatagcatc ttcgatggcg taaaccgaag ccagggcgtg
    16981 tacgccagta cagacgccgc agatacgttc aacgaacgcc cacgcatcgc gcgggtcgcg
    17041 cccttgtagg atgatctcca gcccgcgaaa catggtgccg caggagacgg cattggtgat
    17101 cacattctga tcgttaatat tcacttcgca gcgcatgtgg ccttcgatgc gcgtaatcgg
    17161 gtcgaccacc aggcggcgtc cggcattatt gatggtgtat ccctgagttt cgtactgagt
    17221 gctcatgcct gtttatcctc attgcctggc tgatgttcgg tttctgtagg ttgctggtta
    17281 tgacgtctgc gctggtcaac ggcgctggcg actgcgtgca caccaacagc cgctgccacc
    17341 acaccaagcg cggttaaacc gacggtatcg gcggtggaat gagtacccat ttgcggaata
    17401 tcgaccacgc ggctgtagaa cgaaccgcaa tcccagaaac cattttccgc acagcccagg
    17461 cagccgtgac cagactggat tgggaaagaa acgccatcat tccagcgtgt ggaggaacag
    17521 gcgttatagg tggtaggccc tttgcagccc attttgtaca ggcagtaacc tttgcgggca
    17581 gcgtcatcat cccaactctg gacgaactct ccggcgtcga agtgggcgcg gcgatagcat
    17641 ttatcgtgga ttcgctgacc atagaacatc agcggacggc ccattctgtc gacatctggc
    17701 aagcgatcaa aggtcaccat gtaagtaatg atggcgctca tcacatccgg gatcggcggg
    17761 cagccaggta ctttgataat gggtttgtcg gtgatgactt tgtcgatagg cgttgcctgc
    17821 gtcggattgg gccacgcggc ctgcacgcag ccccaggacg cgcaggttcc ccaggcgata
    17881 atcgcgctgg ctccggcagc ggcacgtttg agtttctcaa taaacggtcg accgctgctg
    17941 atacagaaca tcccctgctc gcccagcggc ggattacctt ctactgcgag gatatatttg
    18001 ccattgtatt gcgtgatgat gtcttcgaag acttcttccg cctgggttcc ggcggcagcc
    18061 atcaaagtat cgtcataatc gagggaaatc agggaaagga tgacgtcctt cgccagtggg
    18121 tgagcggagc ggataaaaga ttcggtacag caggtgcatt ccagaccgtg gatccatacc
    18181 accggaatgc ggggtttgtt ctccagcgcc caggcaatct ttggtgccat tcccgcgcct
    18241 aatcccagcg acgtggcagc cagactacaa tatttgagaa agctgcgccg ggtaacgccc
    18301 tgacgccgca tggcctggta aaatgtttcc tcgttattca tatcgcacgt ctcttctcct
    18361 tgcgacaccg gcaggacacc gggcgcttat ggtcggcaag cgacgtccct gtagcaaggc
    18421 aaaccatcgc ttcacgtgag atgctgaaaa cgaaagctca tccttctgca ctggcgcacg
    18481 tcgccagaaa gtattgttaa taaagcgtag tgaaact
//
LOCUS       DAZPEP010000074        18350 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZPEP010000074 DAZPEP010000000
VERSION     DAZPEP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18350
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.074"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(147..1256)
                     /locus_tag="ACHUT9_002481"
     CDS             complement(147..1256)
                     /locus_tag="ACHUT9_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFR1348928.1"
                     /translation="MTDTTMQLLSQGTDPVKMPDFDILAEGKTLSGVAERLMSLSLTD
                     NRGFEADQLTITLDDADGQLQLPPRGARLTVLIGWKGEPLTEKGTYIVDEIAHEGPPD
                     RLTVSARSADFRAEFNVKREVSWHDVTVERVVSAIAHRYGLKPQISEMLMDIEIDHAD
                     QTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLPSFAITRSSGDRHQ
                     FRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPSKPKKEKSSSREGDYMEGAEGNVF
                     VLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLAHGRAELYPEMHGTVTGFKSEIDN
                     QDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            1414..2598
                     /locus_tag="ACHUT9_002482"
     CDS             1414..2598
                     /locus_tag="ACHUT9_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1348929.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAELFP
                     LNKPTLLPRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSESDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ASCHDCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVIGAIRAKLDSLVTAG
                     KLIGASCWYDVVDNGTTNLRQGRVRIRYKYTPVPPLEDMELYQSFTDEFFGPAFAVLG
                     GA"
     gene            2598..3110
                     /locus_tag="ACHUT9_002483"
     CDS             2598..3110
                     /locus_tag="ACHUT9_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFR1348930.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTLPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRELFLKYGGTIDGTLLRFAGEYYTDAESDLYEVE
                     MRGRVTEIDMGEAKQGEATSHTYAIKNTYYKLSVNDRPLWEIDLLNFIYRKDGRDIVP
                     DRIRSALGLG"
     gene            3165..3530
                     /locus_tag="ACHUT9_002484"
     CDS             3165..3530
                     /locus_tag="ACHUT9_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1348931.1"
                     /translation="MKETKNIDTENTVVADTVKETSERGVKLTQPIERGGEKITYVEI
                     TGAIEQAGSLRDLSLSDVLNLKAESMFTLLSRVTSPRLDEVTIKKMASRDFIQLCVVA
                     VNFLSGADSGEKNEQATEA"
     gene            3539..3694
                     /locus_tag="ACHUT9_002485"
     CDS             3539..3694
                     /locus_tag="ACHUT9_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFR1348932.1"
                     /translation="MCFEHIEDFVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            3681..6488
                     /locus_tag="ACHUT9_002486"
     CDS             3681..6488
                     /locus_tag="ACHUT9_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1348933.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTGQYNNQLERERRALARVT
                     QARERYSRAQETAGKLKTGGALATGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLAKMGIRGSDAGTALRKSLANLSSPPKAAAEALKELGVSVADA
                     KGKMRPMENVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMGLVDAAGDGFLAKLR
                     KELEGARGEAERTAKVMANNLDGNLKSLGSAWEGLRIRISDLVDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLATTATVGSLSLVIGVLHGKLATLRLGFSLLTES
                     MNVVRVLPALWGMVTGSVSLLGGAIGALFSPVSLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDVVRDGIIQVFNWFKSLLSPMESSKETLDKCASA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            6501..6989
                     /locus_tag="ACHUT9_002487"
     CDS             6501..6989
                     /locus_tag="ACHUT9_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1348934.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDERIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(7025..7612)
                     /locus_tag="ACHUT9_002488"
     CDS             complement(7025..7612)
                     /locus_tag="ACHUT9_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1348935.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGCEQIFEDKLSGTKTDRPG
                     LKRALKRLQKGDTLVVWKLDRLGRSMKHLISLVGELRERGINFRSLTDSIDTSSPMGR
                     FFFHVMGALAEMERELIVERTLAGLAAARARGRTGGRRPKLTKEQHEQIARLIKNGHD
                     RKQLAIIYGIGISTIYRYHPAGESIGTIEKSQETK"
     gene            7894..8637
                     /locus_tag="ACHUT9_002489"
     CDS             7894..8637
                     /locus_tag="ACHUT9_002489"
                     /inference="COORDINATES: protein motif:HMM:NF024018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1348936.1"
                     /translation="MCKGSWAYANNKVITDTGCGNICLAGAVVEVIGTRGAMTIRVTT
                     PSTSSGGGITNAQFTYINHGDAYAPGWRRDYNTKNQQPAFALGQTGSTVGNDKAVGWN
                     WNSGVYNANIGGASTLILHFNMNTGSCPAVQFRVNYRNGGIFYRSARDGYGFEADWSE
                     FYTTTRKPSAGDVGAYTQAECNSRFITGIRLGGLLSVQTWNGPGWSDRSGYVVTGSVN
                     SNRDELIDTTQARPIQYCINGTWYNAGSI"
     gene            8640..9173
                     /locus_tag="ACHUT9_002490"
     CDS             8640..9173
                     /locus_tag="ACHUT9_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1348937.1"
                     /translation="MMHLKNISAGNPETKEQYQLTKQFNIKWLYTEDGKNWYEEQKNF
                     QPDTLKMVYDHNGVIICIEKDVSAINPEGASVVEVPDITANRRADISGKWMFKDGVVI
                     KRTYTGEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(9202..9729)
                     /locus_tag="ACHUT9_002491"
     CDS             complement(9202..9729)
                     /locus_tag="ACHUT9_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1348938.1"
                     /translation="MMHLKNIKAGNAKTVEQYELTKKHGVIWLYSEDGKNWYEEVKNF
                     QPDTIKIVYDANNIIVAITKDASTLNPEGYSVVEVPDITANRRADDSGKWMFKDGAVV
                     KRIYTADEQQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTAKPEWPQKPE"
     gene            complement(9731..11767)
                     /locus_tag="ACHUT9_002492"
     CDS             complement(9731..11767)
                     /locus_tag="ACHUT9_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1348939.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQATAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVTLDATHQNHIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPTVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVNYVDDKL
                     KEHEQSRRHPDASLTAKGLVQLSNTTNSTSETLAATPKAVKAVMDETNKKAPLNSPAL
                     TGTPTTPTARQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATT
                     MTNALAGKQPKDATLTALAGLATAADRFPYFTGNDVASLATLTKVGRDILAKSTVAAV
                     IEYLGLQETVNKAGNAVQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNNSDADTD
                     SYMWFETGNNGNEYFKWRHRIIGTRPKDLMNLKWNALSVLVEALFSSEVKISTVNALR
                     IFNSSFGAIFRRSEECLHIIPTRENEGENGNIGPLRPFTLNLRTGRISMGHGLDVTGD
                     IFANRFAINSSTGMWIHMRDQNVILGRNAVSTDGAQALLRQDHADRKFMIGGLGNKQF
                     GIYMINNSRTANGTDGQAYMDNNGNWLCGSQVIPGNYGNFDSRYVKDVRLGSQQYYGV
                     NNWQTWNFQCPSGYVLTGINVQDTGKNSADNIAGVHYRPVQKYINGTWYNVASI"
     gene            complement(11770..12300)
                     /locus_tag="ACHUT9_002493"
     CDS             complement(11770..12300)
                     /locus_tag="ACHUT9_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFR1348940.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPADLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFNGEPGTFKIDVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIQGDIFA
                     AAATYDGEVITIYPDD"
     gene            complement(12293..13189)
                     /locus_tag="ACHUT9_002494"
     CDS             complement(12293..13189)
                     /locus_tag="ACHUT9_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1348941.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIAAYPAEQREVVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEAARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDVVMENDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(13193..13543)
                     /locus_tag="ACHUT9_002495"
     CDS             complement(13193..13543)
                     /locus_tag="ACHUT9_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFR1348942.1"
                     /translation="MTTRYTGMNPDGTGNLNDMAHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSATVIALTRWEPRIALNAIDVVWKAGGHAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(13540..14121)
                     /locus_tag="ACHUT9_002496"
     CDS             complement(13540..14121)
                     /locus_tag="ACHUT9_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1348943.1"
                     /translation="MSAELMRLLSNIIRTGIISEVDEESWCVRVRSGELETGWLRWNT
                     TRAGDFNVWFPPSPGEQVAIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDG
                     AVFRYDADAGALSASGMKTATLQASISVTLNTPVVECTDLLRTATLDVTKGGTMSGNI
                     THSGGDFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(14118..14753)
                     /locus_tag="ACHUT9_002497"
     CDS             complement(14118..14753)
                     /locus_tag="ACHUT9_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFR1348944.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTRRVSMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(14746..15213)
                     /locus_tag="ACHUT9_002498"
     CDS             complement(14746..15213)
                     /locus_tag="ACHUT9_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1348945.1"
                     /translation="MLKTDSLREAMTRSCRWCHANPEKFTIFVESGNIETTGETPSFV
                     YRYQIVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(15285..15758)
                     /locus_tag="ACHUT9_002499"
     CDS             complement(15285..15758)
                     /locus_tag="ACHUT9_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348946.1"
                     /translation="MKVRYILMMALTVLTLLTITLWQTFRLGQEDGHNRYTQAQLDDY
                     SRTLKEAGTIISTASGVLRDVMTARQNRQQEGEIRREQLRNDITTDACARAVPDARFT
                     ERLHQHAERVRAAAVVPFHPGRADRPRTSPAVSGDAIMGKHRDMERQPHGRTGNL"
     gene            complement(15755..16303)
                     /locus_tag="ACHUT9_002500"
     CDS             complement(15755..16303)
                     /locus_tag="ACHUT9_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1348947.1"
                     /translation="MIKLPVKRYAVAAIVALGFSIAPHKLRTSPEAQIRIATWEDCRA
                     TPYRDIAGVMTVGCGSTGHVENRLYTETEVAGRWVNDMQHAENCINQNFSGNAMPQSA
                     FEAMTDAAFNLGCRNLMWFRDKNRNYHRTTIWKHAQAHNWPDMCNRLTDFVNSGGERS
                     QGLVNRRTDFRAWCLRDTGGQS"
     gene            complement(16284..16586)
                     /locus_tag="ACHUT9_002501"
     CDS             complement(16284..16586)
                     /locus_tag="ACHUT9_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1348948.1"
                     /translation="MVTGFFQRVSDWITFWASVTATTIGVMTISEKIALAGLLLGSLS
                     ALHAWRHRRLMETAMQRRNELIASILAQSDARPLHDSERQALNILRDNDTHDKAAR"
     gene            complement(16580..16777)
                     /locus_tag="ACHUT9_002502"
     CDS             complement(16580..16777)
                     /locus_tag="ACHUT9_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFR1348949.1"
                     /translation="MKVKTLEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCRQVFLEA
                     GQEVEMPEPEKKKQREMIQLW"
     gene            complement(16777..17271)
                     /locus_tag="ACHUT9_002503"
     CDS             complement(16777..17271)
                     /locus_tag="ACHUT9_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFR1348950.1"
                     /translation="MSMVARTEPGPAEDDITDTEDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(17373..18173)
                     /gene="gpM"
                     /locus_tag="ACHUT9_002504"
     CDS             complement(17373..18173)
                     /gene="gpM"
                     /locus_tag="ACHUT9_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFR1348951.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKADIKKALLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYAVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSVFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNQNAGVKREIATLS
                     RALKKADSAAAPEDASAQQAQDESSESKKTTRKPATRKTTATQKAKRG"
     gene            complement(18219..>18350)
                     /locus_tag="ACHUT9_002505"
     CDS             complement(18219..>18350)
                     /locus_tag="ACHUT9_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFR1348952.1"
                     /translation="MRRLMKDEPEYNRIATYQSINDAYVVEDYGKCAMVTGLKFADS"
BASE COUNT         3973 a         4823 c         5044 g         4510 t
ORIGIN      
        1 cccctgatgt caggtaggaa aacaccatat gtttctgctc tgtttttcct tagaaatagg
       61 catccttgct ggaacaaagc gagcagctat attgactcta tgttgtgagt gtgttgatcc
      121 gttctgagtt aatacattta agttgctcac tctgtttctg ctatccattc cgggattttt
      181 gcctcaagct caagctgcgt ggtaaagccg ctgttatcaa tggtgtgctc ggcttttgca
      241 ataatccagt cctgattatc aatctcgctt ttaaatcctg ttaccgtgcc atgcatttcg
      301 gggtagagtt ctgcacgtcc atgcgccagt gtgatggaga atgatgcggc tccgcgttgt
      361 agctgctgcc actttgccgc cgctgcacgt cttgctgcct gctcgttctg ataagttttg
      421 cgtaacacaa acacgttgcc ttccgcacct tccatatagt cgccttcccg gctgctgctt
      481 ttctcctttt ttggttttga cggtttgcgg cgtttcacgc tgactttttt ctttttcccg
      541 taattaagat caagccagta agcgcgtacc cccgtatacg cttcgcggtc agcaatgcgg
      601 aactgatggc gatcgccgct gctgcgtgtg atggcgaacg agggcaacgg ctggccctgt
      661 gcgttcacgc caccaccggg catgatgaat aacagattac cgctttttac cgtggtgatt
      721 gcgcccagca tttccgccat gcgcgtaagg aaggacatgt cgctttcttc ggtctggtcg
      781 gcgtggtcga tttcgatatc catcagcatt tcgctgattt gcggtttcag accataccga
      841 tgagcgatgg cggataccac acgctcaacg gtcacatcat gccaggacac ctcacgttta
      901 acgttaaatt cagcccgaaa atctgcgctt ctggctgaaa cagtcagcct gtccggcggt
      961 ccttcgtgag cgatttcatc aacaatgtaa gtgccttttt ctgtcagcgg ttctcccttc
     1021 cagccaatga gaaccgtcag gcgcgcgccc cgtggcggta gctgcaactg accatccgca
     1081 tcatccagcg tgatggtgag ctggtccgcc tcaaatcccc ggttgtcggt cagtgacagg
     1141 ctcatcaggc gctctgccac gcctgacagc gttttaccct ccgcgagaat atcaaaatcc
     1201 ggcattttca cggggtctgt gccctgactg agcaattgca tggtggtgtc ggtcatctgc
     1261 tccctccctg tgtggcatgg tcgcatgtgc gtgcggaggg ggttactgct ttttgttgtc
     1321 gccgtggcgg gagaatggcg caggggtgag attacgcgcg tggtgggtga tgattgttgc
     1381 cgaatcattt aacggataca aggggttgaa gctatgagtg aaactcgttt tcatggtgcc
     1441 cgtgttacgg aaaataccga cctggtaaca gcgattaacg atgttgattc cagcgttatc
     1501 ggtatcgtgg caacggcgga tgatgcggac gcggagctgt tcccgctgaa caagcccaca
     1561 ctgctgcccc gcgtcaatga cgtgctggga aaatgcggaa caacggggac gctttatcgt
     1621 gcgcttaagg ccatcgcaga ccaggtgagc acaaaggtga tcgtcgttcg cgtggctgaa
     1681 cacaaagaag aagacggaaa aacgcaggat caactggtta tcggtggttc tgaatctgac
     1741 ggcagctata cggggatgta tgcgctgctt gttgcagagc aggatgaaag catcggatac
     1801 cgtccgcgta ttctggccgc gccggagctg gacacggagg cggtgacaaa atccctgtgc
     1861 gtgattgcgg gtaaactgcg cgcgtttgtg tatgcctcat gtcatgactg taacacgatg
     1921 gctgaggcaa ttacctaccg ccagaaattc aacgaacgtg aggtgatgct cttatggccg
     1981 gacttcatcg cctacaaccc gaaaagtggc aaaaacgaaa cgttccccgc gcctgcctat
     2041 gcgtgcggcc ttcgtgcgta cattgaccat gagcagggct ggcacaaatc gctgtccaac
     2101 gttccggtta aaaatgtgct ggggatgtcc aggcatgtgt tctggtcgtt gcaggccgaa
     2161 gacagtgatg ccaacagcct caacaacaaa gaaatcacga ccattattcg tcgcaacggg
     2221 ttccgcttct ggggcaaccg cacaccggaa acgaacgcct acatctttga ggtgtatacc
     2281 cgaaccgcac aggtgctggc tgattcaatt gcggaagcgc agtttgaaac catcgacagt
     2341 ccactgacgc ctgcgaacgt gaaggatgtt atcggtgcca tcagggcaaa actggattca
     2401 ctggttactg ccgggaaact gattggggcg tcgtgctggt atgacgtggt ggataacggc
     2461 accacgaatt tacgtcaggg gcgcgtgcgt attcgctaca aatatacgcc tgttcccccg
     2521 ctggaagaca tggagcttta ccagtcgttt actgatgaat tctttggtcc cgcatttgcg
     2581 gtgctgggag gtgcctgatg gctgtaccaa aacatcttcg cttttttacg ctgtttgtgg
     2641 atggtgaaaa cgaagtgggt aaggtgacgt ccgtcactct gcctaagctg acgcgcaaaa
     2701 ccgacagcta ccggggtggt ggcatgatgg gagcggtaag tattgatctc ggcctggatg
     2761 actccgcgct tgatgcgagc tttgtcatgg ggggcgcagt tcgtgagctg ttccttaagt
     2821 atggcggcac gattgacggt acgctgctgc gttttgcggg tgaatactac accgacgctg
     2881 aaagcgacct gtatgaagtc gaaatgcgcg gacgtgtgac ggaaattgat atgggggaag
     2941 ccaaacaggg cgaagccaca tcacacactt acgccattaa aaacacctac tacaagctga
     3001 gtgttaacga tcgcccgttg tgggagattg acctgctgaa cttcatttac cggaaggacg
     3061 gcagggacat tgtgcccgat cgcatccgtt ccgcgctcgg gcttggctga taagtaatat
     3121 gcaggcggcg cagtgcgtcg cctctgactg aaaggagttt cctgatgaaa gagacgaaaa
     3181 acatcgatac cgaaaacacg gtcgttgctg acactgtgaa agaaaccagt gagcgtggcg
     3241 taaaacttac ccaaccaatt gagcgaggcg gcgaaaaaat cacgtatgtg gagatcaccg
     3301 gggctattga gcaggctgga tctctgcgag atttgtcgct gtctgatgtg ctgaatttga
     3361 aagcggaatc catgtttacg ctgctgtcac gcgtgacatc accgcgactg gatgaagtga
     3421 cgatcaaaaa aatggcatcc cgtgacttta ttcagttatg tgtggttgcc gtaaattttt
     3481 tgagcggtgc ggactctggc gagaagaacg aacaggcgac ggaagcctga tcacggttgt
     3541 gtgctttgag cacatagaag actttgtggc ggatattgcc gccattttta actggtcgcc
     3601 cgccgaaatc ttcatgatga cgcccggcga agtggttagc tggcgtgagc gggcggcact
     3661 tcgcagcggg aatgcagaca atgaagactc ttgatatccg ggtcgctttc agcgccgttg
     3721 acaggctgac ccggcctgcc gaaaacgccc gccgcctgat ggggcagttt ggtgactcca
     3781 tccagcgaac gcagggggcg atcaaaaatc tcgagcgtca ggcgcgttca tttgagcgcg
     3841 cccgtgacgc tgtcagtaaa gcggatgcgg gcatcgtgaa agcacgacgc cagcttaacg
     3901 cccttaatca gttacaacgc acgggtacag tgctcagcga aaaacaacaa aagctgatgc
     3961 agcagttaag cacccggctt gaacgcctga atgaatcgcg cacacgggaa attcagaaaa
     4021 tgcgggaact tggcggagag ctgaaacgcc acggcatttc cctgacaggc agcgataaca
     4081 ccatccagca ggccatcaga cgcaccggac aatacaacaa ccagcttgaa cgcgaacggc
     4141 gggcgcttgc gcgtgtaacg caggcgcgtg agcggtattc acgcgcgcag gaaacagcgg
     4201 gaaaactgaa aacaggtggt gcgctggcaa caggtgcggc ggcagcgggc ggctatgctg
     4261 ccgggcgttt tttgcagccc gctatcgggt tcgggaaaga gatgtcccgt gttcaggcac
     4321 tgacgcgaat cgataaaaac agcccgcagt ttaaggcgct gcgtgagcag gcgttaaaac
     4381 ttggctctga aacgcagttc accgcaggcg atgccgccag tggacaggca tttcttgcaa
     4441 tggctggttt cacaccgcag gccattcagg ctgcgcttcc cggcgtgctg agcatggcaa
     4501 cggctggcgg tatggacctc ggcgagacgg cggatattgg ttccaatatc ctgacgcagt
     4561 tcggcctctc tgctgaccag atggaccggg tcggtgacac acttaccgca gcgtttaccc
     4621 gtaccaacac tgaccttcgc gcactgggcg aaaccatgaa atatgcaggt ccggtggcgg
     4681 gtaagctggg aatatcgctg gagcaggccg cagcgatggc gggcgtgctg gcgaagatgg
     4741 gtatcagggg gagtgatgca ggtacagcgt tgcgtaaaag cctggccaat ctgtcatcac
     4801 cgccaaaggc agcggcagaa gcactgaaag agctgggggt gtcagttgct gacgccaaag
     4861 gcaaaatgcg cccgatggag aatgtgctgg ccgatctcta taaagccacc cgcaaatacg
     4921 gggaggttga ccgggtatcg ttctttaagg acattgccgg agaagaggct ttcacatcat
     4981 ttatgggcct cgttgatgcg gcaggtgacg gcttcttagc caaactgaga aaagaacttg
     5041 aaggcgcgcg cggtgaggca gaacgcacgg caaaggttat ggccaataac cttgatggca
     5101 acctgaaatc actcggcagt gcatgggaag gtctgcgtat tcgcatcagt gatctggttg
     5161 acggtccgct gcgttctgtc acgcagtggc tcacgcgggt tgtctcaaag gtgacggcgc
     5221 tggcgcaggc ccacccggca ctgacgcgcc agttactgat agcgggaggc gcactgctgg
     5281 cgacaacagc aacggttggt tcgttgtctc tggttattgg ggtgcttcac gggaagctgg
     5341 ctacgttgcg tctcggtttt tctctcctga ccgaatcaat gaatgttgtc agggttctgc
     5401 ctgcattatg gggaatggtg acgggttccg tttctttact tggtggcgct atcggggcgc
     5461 tgttcagtcc ggttagtctt atcgtggctg cgcttgccgg agctgccgtt cttatctgga
     5521 aatactggga tcccatcagg gcattttttg ccggggtgtt cagcgggatt atggaaaggc
     5581 tgaccccgtt gcgcgaaacc tttgaacggt ttggccctgt ttttgatgtc gtgcgcgatg
     5641 ggattattca ggtctttaac tggtttaaat cgctgctgtc accgatggag tccagcaagg
     5701 aaacgctgga taaatgtgcc agtgctggcg agatattcgg taacgttctt ggcggtgcgt
     5761 tacaacttgt tctgacacct gcaaaaatgc tactggatac gctggcgtgg atacttgaaa
     5821 aacttggcgt ccttccggat gaagcggaaa gggcgcgcaa gaaaatcgaa gacgcacagc
     5881 gtgcggccat tcttcaggac aaggttgcct tgcttcaggg ggacctggcg aaaatcaatc
     5941 cgccgaagcc tgtggaaaat ggcaatggca ccggaggtga taaacccaaa gacaacaaac
     6001 cgctcacaga cagcaatacc gggacgctac gcagactcag caaaattgct gataacacag
     6061 gtaagctggt tgatgagacg aaaaaacgca ttggccccgg cgatattgtc tttaagaacc
     6121 tgccccgcgc acttgctgtt cgtggggagt ggcaggagcg gaagattgcg caggtcagta
     6181 agcctgcccc cgcaattaat atcacacccg tggtcccggc tccgctgcct ccggcgctgg
     6241 tccctgttgt tgcggccagc tcccgcccgg tggcggaggc catacgctct ccagtggcat
     6301 cagttcctgt aacttcccgt aaccgggagc ctgttgcctc cggatttggt ggtgaaattc
     6361 atgttcatct gcataacgtt gttacgcaga atccccgcga actggcgaaa ctggtcggtg
     6421 aaatggtcag ggcagaaatg gaacggcgcg cccgtgccgg gcgtggcagt ttttacgata
     6481 aagattgagg agtcatggcc atgatgatga tctacggcat gtttgttttt gagctgcgta
     6541 cattgccgca tcagcagtta cagcaaaaca aaagctggcg gcatgtgaaa aatgaacgcg
     6601 ttaaccgttc ggcaagctgg cagtatatcg gtgcaggtga tgagcgcatc gttctttctg
     6661 gtgtgcttta tcctgaaatt acaggtggcg aagtgtcgct gtcgctgctg accacgcagg
     6721 cgtatacagg acgaccctgg cctctgattg atggcgtcgg gcagatttac ggcatgtatg
     6781 tcctgaccga aacgaatacg acccgctccg agtttgatcg ctacggtaag gcgaaaaaga
     6841 tagaattttc actgactctt gaacgctgtg atgaggattt gcgggagcgc ctgcaatcct
     6901 catcgttcag cgatatgctg tccggcttca aagataaggt aacatcatcc cttaacagcg
     6961 cggccagttc agttaaaggg ttgttctgat ttaacacaaa aaccgctaat ggtcagatta
     7021 gcggttattt tgtttcctga ctcttctcta ttgttccgat tgattctcct gcggggtggt
     7081 aacgataaat cgtcgatata ccaatgccgt aaattattgc cagttgtttt ctgtcgtgcc
     7141 cgtttttgat cagccttgct atttgctcat gctgttcttt tgtcagcttc ggtcgacgtc
     7201 cgcctgtgcg cccccgtgcg cgtgctgccg ccagtccggc cagtgtacgt tcaacaatta
     7261 attcacgttc catttcagcc agggcaccca tcacgtggaa gaaaaaacgc cccattggag
     7321 aagatgtatc tatgctgtcg gtcagactgc gaaaattaat tcctcgctcc cgtagttccc
     7381 cgacgagaga aatcagatgt ttcatgcttc gcccgaggcg atccagtttc cagacaacca
     7441 gcgtgtcacc tttttgaagg cgctttaaag cgcgttttaa tcccggtcgg tctgtctttg
     7501 tcccgcttaa tttatcttca aatatttgtt cacatcctgc acaaacaaga gcgtttcgtt
     7561 gcaggtctgt attctggtca tttgttgata cccttacata gccaatcagc acgctgaatc
     7621 tcccgtccaa aagcgcaaat catgccatgc aggccggaaa cggccattat ctaaaacctc
     7681 ggtttacagg aaacggtaaa tcaggcttct ggcgcattac agaaaaacca gaacggcgca
     7741 gatattccgg ggaaagatac cttcaccaaa aatattggtg cctgccgcgc atatagctca
     7801 tggctgaata ttggtggcga tagtcaggtc tggacaaccg cgcaatttat ttcgtggctg
     7861 gagagtcagg gagcatttaa ccatccttac tggatgtgca aaggctcatg ggcttatgca
     7921 aataataagg tcattacaga tacaggttgc ggaaatattt gtcttgcagg tgctgtggtg
     7981 gaagttattg gcactcgcgg cgcaatgacc atacgcgtta ccacgccgag cacgtccagc
     8041 ggtggcggaa ttactaacgc tcaattcact tatattaatc atggtgatgc ttacgctcct
     8101 ggctggcgac gagactacaa cacgaaaaat caacaacctg catttgcttt agggcaaaca
     8161 ggaagcactg tcggaaatga taaagctgtt ggctggaact ggaatagcgg ggtctataac
     8221 gcaaacattg gtggcgcatc gacattaatc ctccacttca atatgaatac ggggagctgc
     8281 cctgctgtac agttccgcgt gaattacagg aacggcggta ttttttatcg ttcagcgcgt
     8341 gatggttatg gctttgaagc tgactggtca gagttttaca ccacaacccg caaaccctct
     8401 gcgggagatg ttggtgcata tacgcaggca gaatgtaact caaggtttat tacaggtatt
     8461 cgccttggtg gtctgttatc tgttcagaca tggaatggtc ccggatggtc tgacaggtca
     8521 ggttatgttg ttaccggttc cgttaactca aacagagatg aactaattga tacaactcag
     8581 gcaaggccaa ttcagtattg cattaatggg acgtggtata acgcggggag tatttaacga
     8641 tgatgcactt aaaaaacatt tctgctggta atcctgaaac aaaagagcaa taccagctaa
     8701 cgaaacaatt taacatcaaa tggctttata cagaggatgg gaaaaactgg tatgaggaac
     8761 aaaagaactt tcagcctgat acgttgaaaa tggtctatga ccacaacggc gttattattt
     8821 gtattgaaaa ggatgtttca gcaattaatc cggaaggcgc aagcgtcgtt gaggttcctg
     8881 atattacagc aaatcgccgg gctgatattt caggtaaatg gatgttcaaa gatggcgtag
     8941 tgataaagcg aacttatacc ggggaagagc agaggcaaca agcggaaaat gaaaagcaaa
     9001 gcctgctaca gctcgtcagg gataaaaccc agctatggga ctcacagcta cggctgggca
     9061 ttatttccga cgagaataaa caaaaattaa ccgagtggat gctctatgcg cagaaggtcg
     9121 aatccacaga cacctccagc ctgccagtaa cgtttcccga acaaccagaa tgaaacaagg
     9181 cccgatatcg ggccttaatt tttactcagg cttttgtggc cattctggct ttgccgtatc
     9241 cacacggctg accagaacac tgtagcgttc ccatgcttcc agtcgtgtgc gttcctcgtc
     9301 cgtcgccata ttcagcctga cagcgcgttc cagcggctgg atgactgatt cagcttcgga
     9361 aagcaatgcg gccttttgtg attcggcctg ttgttgctgt tcgtcagccg tataaatccg
     9421 tttaaccaca gctccgtcct taaacatcca cttaccggaa tcatcagcac gacgattagc
     9481 tgtaatatca ggaacctcaa cgacgctata accttcaggg ttaagcgtgg aggcatcttt
     9541 ggtgatggcg acaataatat tatttgcatc gtaaacaatc tttattgtgt ctggctgaaa
     9601 gttcttcact tcctcatacc agttttttcc gtcctcagag taaagccaga tgactccgtg
     9661 cttctttgtt aactcatact gctccactgt tttagcatta cctgctttta tgttctttaa
     9721 gtgcatcata ttaaatactc gccacattat accaagtgcc atttatatac ttttgcactg
     9781 gcctgtaatg aacgcctgca atattatcgg cagagttttt tccggtatcc tgtacgttaa
     9841 tccccgtcag tacatagcct gacgggcact gaaaattcca tgtttgccag ttgttcactc
     9901 cataatattg ctgtgaacca agtcgaacat ctttcacata tctggaatca aaattgccat
     9961 agttgccggg aataacttgc gagccgcaaa gccagttccc gttattatcc atgtacgcct
    10021 gaccatcggt gccattggct gtccttgagt tattaatcat gtagatgcca aattgcttat
    10081 ttcccagacc gccaatcata aatttgcggt cggcatggtc ctgacggagc aaagcctgag
    10141 caccatcagt ggataccgca ttacgtccca aaataacatt ctggtcacgc atatgaatcc
    10201 acatgccggt actactgtta attgcaaaac ggtttgcaaa tatatcccct gtaacatcaa
    10261 gaccatgccc catgcttatc cggccagttc tgagattaag cgtaaagggg cgtagtggcc
    10321 ctatattacc attttcccct tcattctctc gtgtagggat gatatgcagg cattcttcag
    10381 aacgacgaaa aatggcacca aaagatgaat taaatatcct cagtgcattg actgtcgata
    10441 ttttcacttc actgctgaaa agggcttcaa caagaacaga caaagcattc catttaagat
    10501 tcatcaggtc tttaggccgg gtgccaatga tgcggtgtct ccatttgaaa tattcattgc
    10561 cgttgttgcc tgtttcaaac cacatgtagg aatcggtgtc tgcatccgaa ttatttttaa
    10621 acccaatctt tgcccagtca gtatttcgaa tccaggcaag gattgagtcg ttttcaaaag
    10681 taagtccacc ggacaacgta tcgccatttt tttgcacggc gttaccagcc ttgtttaccg
    10741 tttcctgtaa accgaggtat tcgataacgg cggcaacggt cgatttcgca agaatatccc
    10801 gcccgacttt tgtcagggtt gccaggctgg cgacatcatt ccccgtaaaa tacggaaacc
    10861 tgtctgccgc agtagcaagc cccgccagcg ccgtcagagt ggcatctttc ggttgcttac
    10921 ccgcaagcgc gttagtcatg gtggtcgcaa aattcgggtc gttgcccagc gccgccgcca
    10981 gctcgttcag tgtgttcagt gcatcaggcg aagagtctac aagtgcggca atcgcagcca
    11041 taacgaaagc cgtgcttgcg atttgggtat tattcgttcc ctgtcgcgca gttggtgttg
    11101 ttggcgttcc ggtcagtgcc gggctgttta atggggcttt cttgttcgtt tcatccatta
    11161 ctgccttaac ggcttttggt gtcgctgcca gcgtttcaga cgtgctgttg gtggtgttgc
    11221 tgagctggac aaggcctttt gcggtcagcg aggcgtccgg gtgacgtcgt gactgttcat
    11281 gctctttcag tttgtcatcc acgtaattca ctgtggccat caccatggtg ttatccacgg
    11341 taagcgccac agtggcagtg ctggatacgg tcagaatggt gcgaaatgtt tgtgcacgtc
    11401 cggatccttc ggcaacggtt ggcttgtaac tttcggcagt attgcctacc gcgatcaaat
    11461 cgccgtgctc atcaaacaca ccaatttccc ggatccagaa tccgcccgtt tcaggaggaa
    11521 taaccagctc cgcaataatg tggttctgat gtgttgcgtc cagggtgacg cgattaacgg
    11581 tatgtcgcca cacctcatgc acaagacggg tctgcttact gtctggcgtg ggtaaagtac
    11641 cgccgccgtc acccacggcc atatgtgtca ggcggacagg tttcccatct ggcgcggttg
    11701 cctgcgctaa tttttttgca cccgtatcgg tgataatggt tttaaatttt cgtgttgtgg
    11761 tactcatgct taatcgtccg gataaatggt aatgacttca ccgtcgtaag ttgctgctgc
    11821 cgcgaaaata tctccctgaa tctcctgaat gatattcagc cctgtcatgt ggcggctgac
    11881 cgggcgggca tcagcaatca accgctccat ttccagatac atttcctccg tcacgccact
    11941 gtccagcgtg ccaacatcaa tcttgaatgt tccgggttcg ccgttgaact cccaccactc
    12001 agacacgcga atgaggtatc ccagcggctc aatggcccgt cgcagggcgc tgatggttcc
    12061 tttgtgtcgg tgtatcagcc atgcatcacg aatcacctgt cgctttgtct cttccggcca
    12121 gttgcgatcc cagcggtcaa cggaaaacgc ccaggcgaga taaggcagca gatgcaccgg
    12181 gcaggtgtcc ggcgaccaca gcgtgttgag gtctgccgga atgtctgtaa tgcgtgttcc
    12241 gacggcttcg gcacaacgca tgaaattgct ggctgatggt ggtaacagtg aattactcat
    12301 tacgcccacc ttcgctgatg gtgaatgact cacagcgcgc cgcctgtatg tcgctgatgg
    12361 ccatattctg tgtgggttcg attatctcca cgcgttgcac accgtgcaca tgcagtgcgg
    12421 cagcaatggc ggacaacgcc acgtcctgac cgataagccc ctgctcagcc agccacttcc
    12481 tgaacgacga ttcagccgca gccagaatag gttcggattc cggaccgggg taaaagtaca
    12541 gttttgcatt cagccgccat gtcacgattc tggcactctg tacggtcagg cggtcggcca
    12601 ccgggcgggt atcctctgca ttcagaacgg cgcgaacggt attaagcaac gcctccgttg
    12661 ctgtgccgtc gccctcagtg gacaggatgg aaaccgtcac atttgccgga gacggactga
    12721 tggcccgcgc atcgcgcacc agaccgctgg cgctgcgggc aaaatactcg tatgcgcctg
    12781 acgggcctgc aacactcagg ccgtcatacg cccgctgcgc ccgcagtctc agcgaggtgt
    12841 cgttctccat caccacgtcg gtggtatccg ttgccggagt gataaccagg cgctttgtgt
    12901 tcatattgcc cgcgaggttg tccaggtctg ttccggcgct gtggctcaac atgcaggcgc
    12961 gtgcggcctc attgacccgc tggcgtaaca gcatttcacg aaacgacatg gtttgagcga
    13021 taacgttcag gggttccgat tccagttcca gtgcggcgga gacgacttca cgctgttcgg
    13081 cgggataagc tgcaatcatc atggcctttg tgtcagccag aattgcctca aagtcaggct
    13141 ccgcgatgat ggcgggttcc ggtaactggg aaaggtcaac ggcaggcatg atttactccc
    13201 tcagcgtgat ggttaattca acattctgca tggtctgcat gacagtgccc gacagcgtca
    13261 ccccggcgtg gcctcctgcc ttccagacaa cgtcgatggc gttcagggca atgcgtggtt
    13321 cccatcgtgt cagtgcaatc acggtagcac tcatgcattg cagacgcgtg gtgttattca
    13381 tgggttcgtc aatcaaatca ggcacaaggc tgccatattc ccgtcgcata acccggcttg
    13441 ccagcggggt ggtcaggatg tccctgactg actgtttcag gtgcgccata tcgttcaggt
    13501 ttcccgtccc gtccgggttc attcctgtgt agcgggttgt cactgcgggc ctcctgtcga
    13561 atcgctgcca cctttaacgc caccgtgctt atgcgtatgc actgtgatgc cgtttgaggt
    13621 gaaatcgccg ccgctgtgcg tgatattgcc gctcatcgtt cccccttttg tgacgtcaag
    13681 cgtcgctgtt ctcagaaggt ctgtgcattc cacgacaggc gtgttcagtg tcacgctgat
    13741 ggatgcctgc aaagtggccg ttttcatgcc gcttgcgctc agtgcgcctg cgtccgcgtc
    13801 gtagcggaac accgcgccgt ccggcgcgct gaccacgatt tctttcaggc ttttgccggg
    13861 tgccggaatg gcatcactcc acaggctgcc gattatcatg gcggtttccg ggttgccgcc
    13921 gatgcaggca attgccacct gttcgcctgg tgatggcgga aaccacacgt tgaagtctcc
    13981 cgcgcgcgtg gtgttccagc gcagccagcc tgtttccagt tcgccgctgc gaacgcgcac
    14041 gcaccaggat tcctcatcaa cttcagagat gatcccggtg cggatgatat tgctcagcag
    14101 tcgcatgagt tctgcgctca tcgtacagcc tccgcaatcc ggcccagcac cgtgttataa
    14161 atcaggcgct catctgcctg gctgataccc agcagctcac gtaccgggta atcggtgaaa
    14221 atgcccggcg caacctgatc gcgctcaccg aactgatgaa cgcgtgcaat acgtgcggcc
    14281 acgccgctgt aacccaccgt cacaccggaa gcatctgcac gggctttcag gtagcgggcg
    14341 gtgcgcagtt ttacgaacat ggagacgcgc ctggtgctgt cctggttgat gcgccgggtg
    14401 cgtatttcca gaaaacggtc gatgtcatcc cggtaaaacg tgcggatatt gtttttatcc
    14461 tcatcccacc cggtgatggt tcgcccgtat ttccccgtgt cgtgatgcca gtttttcagc
    14521 gtgcgtgctt cgttattcca gataaagcga atgcgctcct gtatccgggt tacgcggcgt
    14581 ctgcgtggtg tccatgcggt cccgtccggc gctttctgtg accggatacg cgcctgctgg
    14641 gcgcggcgta aatcctgtgc cagctttctg gcgatgttat taatggcctg ctgattcagg
    14701 ctgtcgcgga tagcctcaaa ggtttcatcc acgcgggtga atgccttatc catcgctttc
    14761 cccccacgtc acatcctgga atacatgcga ccagtcgcct tcggaagagg gcaggcgggg
    14821 ttttggctcc ggcaggtgtt ctgcctgcgg tgtgccctga ctgctgcgcg tgatgcgaac
    14881 gcgttcccgc agggggagcg taaacaggag atcggcgctg tcatcgtcat tgataacggc
    14941 ggaaaatttg atgtcctgat tacgctccgg attgagcaac aactgtggct gattttcgga
    15001 taaccacgcc agcagcggca gcgtgaggtc gtccagctct ccggcgtaat ccatgacaaa
    15061 catcactatc tgatagcggt aaacaaacga tggggtttct ccggtcgttt caatgttgcc
    15121 gctctccacg aaaatggtga atttttccgg gttggcatga caccaccggc atgaacgggt
    15181 catggcttca cgcagggaat cagttttcag catggttgtt gtcctcgttg ttcagtcgtt
    15241 gcagcctgcg ctgttccagt aattcaatgg cccgcttatc ggcgttacag gtttccagtg
    15301 cgtccatgag gctgtcgctc catatcccga tgtttcccca tgatggcgtc accggaaacg
    15361 gcgggggaag tacgggggcg gtcagccctt ccgggatgaa acggaacgac ggcggcggcg
    15421 cggacgcgtt ctgcgtgctg gtgcaaccgc tcagtaaagc gagcgtcagg aacagcgcgg
    15481 gcacatgcat ctgtcgtgat atcgttgcgt agctgttcac gtcggatttc cccttcctgt
    15541 tgtcggttct ggcgcgctgt catgacgtca cgcagtacac cggatgcggt gctgatgatg
    15601 gtgccggcct ctttcagggt ccggctgtaa tcgtccagtt gcgcctgtgt ataacggttg
    15661 tgtccgtctt cctgccccag tctgaatgtc tgccagagtg ttatggtcag cagcgtgagg
    15721 acggtcagtg ccatcatcag gatgtaacgc actttcatga ctggcctccg gtgtcacgta
    15781 agcaccatgc acgaaaatca gtacgacggt taaccagtcc ctgtgagcgt tcgccgccgc
    15841 tgttcacgaa gtcagtcagt ctgttgcaca tgtcaggcca gttgtgcgcc tgtgcatgtt
    15901 tccagatagt ggtgcggtgg tagttacggt tcttatccct gaaccacata agatttctgc
    15961 atccaagatt aaaggcggca tctgtcattg cctcaaacgc ggactgtggc atggcgttgc
    16021 cgctgaagtt ctgatttatg cagttttctg catgttgcat atcattaacc cagcgcccgg
    16081 cgacttccgt ttcggtgtac agacgatttt ccacatgccc ggtcgagccg caccccacgg
    16141 tcattacccc ggcaatatcc cggtaaggcg ttgcgcgaca gtcctcccat gtggcaatgc
    16201 ggatttgcgc ttccgggctg gtccggagtt tgtgaggggc aatggagaac cccagcgcca
    16261 caatggcggc tacggcgtaa cgtttaacgg gcagctttat catgtgtatc gttatcccgc
    16321 agaatgttca gagcctgacg ctcactgtcg tgaagtggcc gtgcgtctga ctgcgccagg
    16381 atactggcga tgagttcatt gcgacgctgc atggcggttt ccatcaggcg gcgatgtcgc
    16441 caggcatgta atgcagacag agaaccgagt aacaacccgg caagggcgat tttttcactg
    16501 atggtcatca cgcctattgt ggtggctgtg actgatgccc agaaggtgat ccagtcactc
    16561 acccgctgaa aaaaacctgt taccatagct gtatcatctc tcgttgtttt ttcttctccg
    16621 gctccggcat ttccacctcc tgtccggctt ccagaaatac ctgtcggcag agtccggggt
    16681 tggcatccag caccttttcg gtgacgccct gcgtcgtgcc gtagtaccgg aaacagagcg
    16741 aatccacggt gtcgccttcc agtgtcttca ctttcatcag cacaactccg caaagattcg
    16801 cgggcggcac agaatgtcag agatggccca gctcacatcg cgccacaaat ccgatgtctg
    16861 tatatccagt gcgtccgccc ggcggtcgcc cttgtccgtt gtgtccgcat cgcggtaacg
    16921 ctccagaatc agggcgcgtg tggcggtata aacagcattg cgccagtgcc agagattgac
    16981 gctttctccg ttaattacag gtgccggaac atcggccagc gtctgatggc cagccgcctg
    17041 ctgttcctgc tgccatgctt ccagctcgcg ggtaacgtgt gccacggccc cggtggcggt
    17101 atgcagcagg cgggaggtgg tcacgcggcc cggcagtcgt accgccagac gcagctcgcg
    17161 cagcacaata tccggccaga atgcacccgc tgaaatacgg gtatcgccat cttcggtgtc
    17221 ggtgatgtcg tcctctgcgg gtccgggttc agttctggca accatactca tggggttcac
    17281 tcctgaaaaa atcgggcggt gggtgcgcgg tgtaaacggt cacggagcca aaccggaaca
    17341 ccgcgcacgc cgcccgctga cggggtcagt cgttaaccgc gcttcgcctt ctgcgtcgcg
    17401 gtggtttttc gtgttgcagg cttccgcgtt gtctttttac tttcgctgct ttcgtcctgc
    17461 gcctgctgtg cgctggcgtc ttctggtgcg gctgcggaat cggctttttt cagggcgcgg
    17521 gaaagggttg caatctcgcg tttcacacct gcgttctggt tcagatgcat cgcttcgcgc
    17581 agcagcttca gtgatgaggc catgctgtcc gcatcgctca ggccacggcg ggcaaaggcg
    17641 cacgccttgc ataatttggc gcgcacttcg tccggcatat cctggttggc gacaatttcc
    17701 caaagtgtgt ccagtggttc gataaagacg gacaaatccg cgtcggcatc cgtcccggcc
    17761 tgcgtcagta ccggattgca gatttcttcg gtcagcactg tggcagcagt acggccaaag
    17821 ttatccggca tgatgaggtt gtgacggacc gcatacgcac caatacgcag cgcaagcgga
    17881 agatcgccgc agtcaatcgc ccacaccatc agcgtggcaa tcacttcgtc ctgttgcccg
    17941 ccgtcagcct ccagcgttcc ctcaatccag ccggaaaagt ccggcaacaa cgcttttttg
    18001 atgtcggctt tcgcgcttct ggcctgtacg cccttaagcc gggcctgtgc cagacgcaga
    18061 cgatacagca cctcttcatg cgcggtacgc gcggcgtggt ccacaccttc attcgcccgg
    18121 cctgcgcgct gtgccatcac gttctgccag tgttgctgtg caggagtaat cattttttct
    18181 ctccgttaca ggcgggcatg atgcccgccg tgagttgatt agctgtcggc gaacttcagg
    18241 ccagtgacca tcgcgcactt gccatagtct tcaacgacat aagcgtcatt gatggactgg
    18301 taggtggcga tgcggttgta ttccggttcg tctttcatca gacgacgcat
//
LOCUS       DAZPEP010000075        18213 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZPEP010000075 DAZPEP010000000
VERSION     DAZPEP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18213
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.075"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            15..1091
                     /locus_tag="ACHUT9_002506"
     CDS             15..1091
                     /locus_tag="ACHUT9_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1348953.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="ACHUT9_002507"
     CDS             complement(1484..1825)
                     /locus_tag="ACHUT9_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFR1348954.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUT9_002508"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="ACHUT9_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFR1348955.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUT9_002509"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="ACHUT9_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFR1348956.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="ACHUT9_002510"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="ACHUT9_002510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFR1348957.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUT9_002511"
     CDS             3559..4215
                     /gene="yobB"
                     /locus_tag="ACHUT9_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFR1348958.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUT9_002512"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="ACHUT9_002512"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFR1348959.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUT9_002513"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="ACHUT9_002513"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFR1348960.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="ACHUT9_002514"
     CDS             complement(7167..7826)
                     /locus_tag="ACHUT9_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFR1348961.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVSGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUT9_002515"
     CDS             complement(8153..8509)
                     /gene="yebF"
                     /locus_tag="ACHUT9_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFR1348962.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUT9_002516"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="ACHUT9_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFR1348963.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="ACHUT9_002517"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="ACHUT9_002517"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFR1348964.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUT9_002518"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="ACHUT9_002518"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFR1348965.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUT9_002519"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="ACHUT9_002519"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFR1348966.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUT9_002520"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="ACHUT9_002520"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFR1348967.1"
                     /translation="MAVTQTTQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUT9_002521"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="ACHUT9_002521"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFR1348968.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUT9_002522"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="ACHUT9_002522"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFR1348969.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="ACHUT9_002523"
     CDS             complement(17270..18112)
                     /locus_tag="ACHUT9_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFR1348970.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4671 a         4574 c         4393 g         4575 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ccaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gataccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcgggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggttgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc gccgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaa
//
LOCUS       DAZPEP010000076        17821 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZPEP010000076 DAZPEP010000000
VERSION     DAZPEP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17821
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.076"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHUT9_002524"
     CDS             complement(86..1369)
                     /gene="gltA"
                     /locus_tag="ACHUT9_002524"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFR1348971.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1575..1682)
                     /locus_tag="ACHUT9_002525"
     CDS             complement(1575..1682)
                     /locus_tag="ACHUT9_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1348972.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHUT9_002526"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2063..2467
                     /gene="sdhC"
                     /locus_tag="ACHUT9_002526"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1348973.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHUT9_002527"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2461..2808
                     /gene="sdhD"
                     /locus_tag="ACHUT9_002527"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFR1348974.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHUT9_002528"
     CDS             2808..4574
                     /gene="sdhA"
                     /locus_tag="ACHUT9_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFR1348975.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHUT9_002529"
     CDS             4590..5306
                     /gene="sdhB"
                     /locus_tag="ACHUT9_002529"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFR1348976.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5607..8408
                     /gene="sucA"
                     /locus_tag="ACHUT9_002530"
     CDS             5607..8408
                     /gene="sucA"
                     /locus_tag="ACHUT9_002530"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFR1348977.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8423..9640
                     /gene="odhB"
                     /locus_tag="ACHUT9_002531"
     CDS             8423..9640
                     /gene="odhB"
                     /locus_tag="ACHUT9_002531"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFR1348978.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9734..10900
                     /gene="sucC"
                     /locus_tag="ACHUT9_002532"
     CDS             9734..10900
                     /gene="sucC"
                     /locus_tag="ACHUT9_002532"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFR1348979.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10900..11769
                     /gene="sucD"
                     /locus_tag="ACHUT9_002533"
     CDS             10900..11769
                     /gene="sucD"
                     /locus_tag="ACHUT9_002533"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFR1348980.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHUT9_002534"
     CDS             complement(11873..12595)
                     /gene="mngR"
                     /locus_tag="ACHUT9_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFR1348981.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12764..14680
                     /gene="mngA"
                     /locus_tag="ACHUT9_002535"
     CDS             12764..14680
                     /gene="mngA"
                     /locus_tag="ACHUT9_002535"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFR1348982.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14698..17331
                     /gene="mngB"
                     /locus_tag="ACHUT9_002536"
     CDS             14698..17331
                     /gene="mngB"
                     /locus_tag="ACHUT9_002536"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFR1348983.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLQDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVIR
                     YDMAVPLNLSERSARQSTGRVGGVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPTP
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4331 a         4415 c         4816 g         4259 t
ORIGIN      
        1 ttacaatttt ttgtgtttgg gcatataaaa attaacccgc catttgaacg gcgggttaaa
       61 atatttacaa cttagcaatc aaccattaac gcttgatatc gcttttaaag tcgcgttttt
      121 catatcctgt atacagctga cgcggacggg caatcttcat accgtcactg tgcatttcgc
      181 tccagtgggc gatccagcca acggtacgtg ccattgcgaa aatgacggtg aacatggaag
      241 acggaatacc catcgctttc aggatgatac cagagtagaa atcgacgttc gggtacagtt
      301 tcttctcgat aaagtacggg tcgttcagcg cgatgttttc cagctccata gccacttcca
      361 gcaggtcatc tttggtgccc agctctttca gaacttcatg gcaggtttca cgcattacgg
      421 tggcgcgcgg gtcgtaattt ttgtacacgc ggtgaccgaa gcccatcagg cggaaagaat
      481 catttttatc tttcgcacga cgaacaaatt ccggaatgtg tttaacggag ctaatttctt
      541 ccagcatttt cagcgccgct tcgttagcac caccgtgcgc aggtccccac agtgaagcaa
      601 tacctgctgc gatacaggca aacgggttcg cacccgaaga gccagcggta cgcacggtgg
      661 aggtagaggc gttctgttca tggtcagcgt gcaggatcag aatacggtcc atagcacgtt
      721 ccagaatcgg attaacttca tacggttcgc acggcgtgga gaacatcata ttcaggaagt
      781 taccggcata ggagagatcg ttgcgcggat aaacaaatgg ctgaccaatg gaatacttgt
      841 aacacatcgc ggccatggtc ggcattttcg acagcaggcg gaacgcggca atttcacggt
      901 gacgaggatt gttaacatcc agcgagtcgt gatagaacgc cgccagcgcg ccggtaatac
      961 cacacatgac tgccattgga tgcgagtcgc gacggaaagc atggaacaga cgggtaatct
     1021 gctcgtggat catggtatga cgggtcaccg tagttttaaa ttcgtcatac tgttcctgag
     1081 tcggtttttc accattcagc aggatgtaac aaacttccag gtagttagaa tcggtcgcca
     1141 gctgatcgat cgggaaaccg cggtgcagca aaataccttc atcaccatca ataaaagtaa
     1201 ttttagattc gcaggatgcg gttgaagtga agcctgggtc aaaggtgaac acaccttttg
     1261 aaccgagagt acggatatca ataacatctt gacccagcgt gcctttcagc acatccagtt
     1321 caacagctgt gtccccgttg agggtgagtt ttgcttttgt atcagccatt taaggtctcc
     1381 ttagcgcctt attgcgtaag actgccggaa cttaaatttg ccttcgcaca tcaacctggc
     1441 tttacccgtt tgttatttgg ctcgccgctc tgtgaaagag gggaaaacct gggtacagag
     1501 ctctgggcgc ttgcaggtaa aggatccatt gatgacgaat aaatggcgaa tcaagtactt
     1561 agcaatccga attattaaac ttgtctacca ctaataactg tcccgaatga attggtcaat
     1621 acttccacac tgttacataa gttaatctta ggtgaaatac cgacttcata acttttacgc
     1681 attatatgct tttcctggca atgtttgtaa caactttgtt gaatgattgt caaattagat
     1741 gattaaaaat taaataaatg ttgttatcgt gacctggatc actgttcagg ataaaacccg
     1801 acaaactata tgtaggttaa ttgtaatgat tttgtgaaca gcctatactg ccgccaggtc
     1861 tccggaacac cctgcaatcc cgagccaccc agcgttgtaa cgtgtcgttt tcgcatctgg
     1921 aagcagtgtt ttgcatgacg cgcagttata gaaaggacgc tgtctgaccc gcaagcagac
     1981 cggaggaagg aaatcccgac gtctccaggt aacagaaagt taacctctgt gcccgtagtc
     2041 cccagggaat aataagaaca gcatgtgggc gttattcatg ataagaaatg tgaaaaaaca
     2101 aagacctgtt aatctggacc tacagaccat ccggttcccc atcacggcga tagcgtccat
     2161 tctccatcgc gtttccggtg tgatcacctt tgttgcagtg ggcatcctgc tgtggcttct
     2221 gggtaccagc ctctcttccc ctgaaggttt cgagcaagct tccgcgatta tgggcagctt
     2281 cttcgtcaaa tttatcatgt ggggcatcct taccgctctg gcgtatcacg tcgtcgtagg
     2341 tattcgccac atgatgatgg attttggcta tctggaagaa acattcgaag cgggtaaacg
     2401 ctccgccaaa atctcctttg ttattactgt cgtgctttca cttctcgcag gagtcctcgt
     2461 atggtaagca acgcctccgc attaggacgc aatggcgtac atgatttcat cctcgttcgc
     2521 gctaccgcta tcgtcctgac gctctacatc atttatatgg tcggtttttt cgccaccagt
     2581 ggcgaactga catatgaagt ctggatcggt ttcttcgcct ctgcgttcac caaagtgttc
     2641 accctgctgg cgctgttttc tatcttgatt catgcctgga tcggcatgtg gcaggtgttg
     2701 accgactacg ttaaaccgct ggcattgcgc ctgatgctgc aactggtgat tgtcgttgca
     2761 ctggtggttt acgtgattta tggattcgtt gtggtgtggg gtgtgtgatg aaattgccag
     2821 tcagagaatt tgatgcagtt gtgattggtg ccggtggcgc aggtatgcgc gcggcgctgc
     2881 aaatttccca gagcggccag acctgtgcgc tgctctctaa agtcttcccg acccgttccc
     2941 ataccgtttc tgcgcaaggc ggcattaccg ttgcgctggg taatacccat gaagataact
     3001 gggaatggca tatgtacgac accgtaaaag ggtcggacta tatcggtgac caggacgcga
     3061 ttgaatatat gtgtaaaacc gggccggaag cgattctgga actcgaacac atgggcctgc
     3121 cgttctcgcg tcttgatgat ggtcgtatct atcaacgtcc gtttggcggt cagtcgaaaa
     3181 acttcggcgg cgagcaggcg gcacgcactg cggcggcggc tgaccgtacc ggtcacgcac
     3241 tgttgcacac gctttatcag cagaacctga aaaaccacac caccattttc tccgagtggt
     3301 atgcgctgga tctggtgaaa aaccaggatg gcgcggtggt gggttgtacc gcactgtgca
     3361 tcgaaaccgg tgaagtggtt tatttcaaag cccgcgctac cgtgctggcg actggcgggg
     3421 cagggcgtat ttatcagtcc accactaacg cccacattaa cactggcgac ggtgtcggca
     3481 tggctatccg cgcaggcgta ccggtgcagg atatggaaat gtggcaattc cacccgaccg
     3541 gcattgccgg tgcgggcgta ctggtcaccg aaggttgccg tggtgaaggc ggttatctgc
     3601 tgaacaaaca tggcgaacgc tttatggagc gttatgcgcc gaacgccaaa gacctggcgg
     3661 gccgtgatgt ggtggcgcgt tccatcatga ttgaaatccg tgaaggtcgc ggctgtgatg
     3721 gtccgtgggg gccacacgcg aaactgaaac tcgatcacct gggtaaagaa gttctcgaat
     3781 cccgtctgcc gggtatcctg gagctttccc gtacctttgc tcacgtcgat ccggtgaaag
     3841 agccgattcc ggttatccca acctgtcact acatgatggg cggtattccg accaaagtga
     3901 ccggtcaggc gctgacggtg aacgagaaag gcgaagatgt ggttgttccg gggctgtttg
     3961 ccgttggtga aatcgcttgt gtatcggtac acggcgctaa ccgtctgggc ggcaactcgc
     4021 tgctggacct ggtggtcttt ggtcgtgcgg caggtctgca tctgcaagag tctatcgccg
     4081 aacagggcgc attgcgcgat gccagcgagt ctgatgttga agcatctctg gatcgtctga
     4141 accgctggaa caataatcgt aacggtgaag atccggtggc gattcgtaaa gcgctgcaag
     4201 aatgtatgca gcataacttc tcggtcttcc gtgaaggtga tgcgatggcg aaagggcttg
     4261 agcagttgaa agtgatccgc gagcgtctga aaaatgcccg tctggatgac acttccagcg
     4321 agttcaacac ccagcgcgtt gagtgcctgg aactggataa cctgatggaa acggcgtatg
     4381 caacggctgt ttctgccaac ttccgtaccg aaagccgtgg cgcacatagc cgcttcgact
     4441 tcccggatcg tgatgatgaa aactggctgt gccactccct gtatctgcca gagtcggaat
     4501 ccatgacgcg ccgaagcgtc aacatggaac cgaaactgcg cccggcattc ccgccgaaga
     4561 ttcgtactta ctaatgcgga gacaggaaaa tgagactcga gttttcaatt tatcgctata
     4621 acccggatgt tgatgatgct ccgcgtatgc aggattacac cctggaagcg gaagaaggtc
     4681 gcgacatgat gctgctggat gcgcttatcc agctgaaaga gaaagatccc agcctgtcgt
     4741 tccgccgctc ctgccgtgaa ggtgtgtgcg gttccgacgg tctgaacatg aacggcaaga
     4801 atggtctggc ctgtattacc ccgatttcgg cactcaacca gccgggcaag aagattgtga
     4861 ttcgcccgct gccaggttta ccggtgatcc gcgatttggt ggtagacatg ggacaattct
     4921 atgcgcaata tgagaaaatt aagccttacc tgttgaataa tggacaaaat ccgccagctc
     4981 gcgagcattt acagatgcca gagcagcgcg aaaaactcga cgggctgtat gaatgtattc
     5041 tctgcgcatg ttgttcaacc tcttgtccgt ctttctggtg gaatcccgat aagtttatcg
     5101 gcccggcagg cttgttagcg gcatatcgtt tcctgatcga tagccgtgat accgagactg
     5161 acagccgcct cgacggtttg agcgatgcat tcagcgtatt ccgctgtcac agcatcatga
     5221 actgcgtcag tgtatgtccg aaggggctga acccgacgcg cgccatcggc catattaagt
     5281 cgatgttgtt gcaacgtaat gcgtaaaccg taggcctgat aagacgcgca agcgtcgcat
     5341 caggcaacca gtgccggatg cggcgtgaac gccttatccg gcctacaagt cattacccgt
     5401 aggcctgata agcgcagcgc atcaggcgta acaaagaaat gcaggaaatc tttaaaaact
     5461 gcccctgaca ctaagacagt ttttaaaggt tccttcgcga gccactacat agacaagagc
     5521 tcgcaagtga accccggcac gcacatcact gtgcgtggta gtatccacgg cgaagtaagc
     5581 ataaaaaaga tgcttaaggg atcacgatgc agaacagcgc tttgaaagcc tggttggact
     5641 cttcttacct ctctggcgca aaccagagct ggatagaaca gctctatgaa gacttcttaa
     5701 ccgatcctga ctcggttgac gctaactggc gttcgacgtt ccagcagtta cctggtacgg
     5761 gagtcaaacc ggatcaattc cactctcaaa cgcgtgaata tttccgccgc ctggcgaaag
     5821 acgcttcacg ttactcttca acgatctccg accctgacac caatgtgaag caggttaaag
     5881 tcctgcagct cattaacgca taccgcttcc gtggtcacca gcatgcgaat ctcgatccgc
     5941 tgggactgtg gcagcaagat aaagtggccg atctggatcc gtctttccac gatctgaccg
     6001 aagcagactt ccaggagacc ttcaacgtcg gttcatttgc cagcggcaaa gaaaacatga
     6061 aactcggcga gctgctggaa gccctcaagc aaacctactg tggcccgatt ggtgccgagt
     6121 atatgcacat taccagcacc gaagaaaaac gctggatcca acagcgtatc gagtctggtc
     6181 gcgcgacttt caatagcgaa gagaaaaaac gcttcttaag cgaactgacc gccgctgaag
     6241 gccttgaacg ttacctcggc gcaaaattcc ctggcgcaaa acgcttctcg ctggaaggcg
     6301 gtgacgcgtt aatcccgatg cttaaagaga tgatccgcca cgctggcaac agcggcaccc
     6361 gcgaagtggt tcttgggatg gcgcaccgtg gtcgtctgaa cgtgctggtg aacgtgctgg
     6421 gtaaaaaacc gcaagacttg ttcgacgagt ttgccggtaa acataaagaa cacctcggca
     6481 cgggtgacgt gaaataccac atgggcttct cgtctgactt ccagaccgat ggcggcctgg
     6541 tgcacctggc gctggcgttt aacccgtctc accttgagat tgtaagcccg gtagttatcg
     6601 gttctgttcg tgcccgtctg gacagacttg atgagccgag cagcaacaaa gtgctgccaa
     6661 tcaccatcca cggtgacgcc gcagtgaccg ggcagggcgt ggttcaggaa actctgaaca
     6721 tgtcgaaagc gcgtggttat gaagttggcg gtacggtacg tatcgttatc aacaaccagg
     6781 ttggtttcac cacctctaat ccactggatg ctcgttctac accgtactgt actgatatcg
     6841 gtaagatggt tcaggcaccg attttccacg ttaacgcgga tgatccggaa gccgttgcct
     6901 ttgtgacccg tctggcgctc gatttccgta acacctttaa acgtgatgtc ttcatcgacc
     6961 tggtgtgcta ccgccgtcac ggccacaacg aagccgacga gccgagcgca acccagccgc
     7021 tgatgtatca gaaaatcaaa aaacatccga caccgcgcaa aatctacgct gacaagctgg
     7081 agcaggaaaa agtggcgacg ctggaagatg ccaccgagat ggttaacctg taccgcgatg
     7141 cgctggatgc tggcgattgc gtagtggcag agtggcgtcc gatgaacatg cactctttca
     7201 cctggtcgcc gtacctcaac cacgaatggg acgaagagta cccgaacaaa gttgagatga
     7261 agcgcctgca ggagctggcg aaacgcatca gcacggtgcc ggaagcagtt gaaatgcagt
     7321 ctcgcgttgc caagatttat ggcgatcgcc aggcgatggc tgccggtgag aaactgttcg
     7381 actggggcgg tgcggaaaac ctcgcttacg ccacgctggt tgacgaaggc attccggttc
     7441 gcctgtcggg tgaagactcc ggtcgcggta ccttcttcca ccgccacgcg gtgatccaca
     7501 accagtctaa cggttccact tacacgccgt tgcaacacat ccataacggg cagggcgcgt
     7561 tccgcgtctg ggactccgta ctgtctgaag aagcagtgct ggcgtttgaa tacggttatg
     7621 ccaccgcaga accacgcact ctgaccatct gggaagcgca gttcggtgac ttcgccaacg
     7681 gtgcgcaggt ggttatcgac cagttcatct cttctggcga acagaaatgg ggccggatgt
     7741 gtggtctggt gatgttgctg ccgcacggtt acgaagggca ggggccggag cactcctccg
     7801 cgcgtctgga acgttatctg caactttgtg ctgagcaaaa catgcaggtt tgcgttccgt
     7861 ctaccccggc acaggtttac cacatgctgc gtcgtcaggc gctgcgcggg atgcgtcgtc
     7921 cgctggtagt gatgtcgccg aaatccctgc tgcgtcatcc gctggcggtt tccagcctcg
     7981 aagaactggc gaacggcacc ttcctgccag ccatcggtga aatcgacgag cttgatccga
     8041 agggcgtgaa gcgcgtagtg atgtgttctg gtaaggttta ttacgacctg ctggaacagc
     8101 gtcgtaagaa caatcaacac gatgtcgcca ttgtgcgtat cgagcaactc tacccgttcc
     8161 cgcataaagc gatgcaggaa gtgttgcagc agtttgctca cgtcaaggat tttgtctggt
     8221 gccaggaaga gccgctcaac cagggcgcat ggtactgcag ccagcatcat ttccgtgaag
     8281 tgattccgtt tggggcttct ctgcgttatg caggccgcac ggcctccgcc tctccggcgg
     8341 tagggtatat gtccgttcac cagaaacagc aacaagatct ggttaatgac gcgctgaacg
     8401 tcgaataaat aaaggataca caatgagtag cgtagatatt ctggtccctg acctgcctga
     8461 atccgtagcc gatgccaccg tcgcaacctg gcataaaaaa cccggcgacg cagtcgtacg
     8521 tgatgaagtg ctggtagaaa tcgaaactga caaagtggta ctggaagtac cggcatcagc
     8581 agacggcatt ctggatgcgg ttctggaaga tgaaggtaca acggtaacgt ctcgtcagat
     8641 ccttggtcgc ctgcgtgaag gcaacagcgc cggtaaagaa accagcgcca aatctgaaga
     8701 gaaagcgtcc actccggcgc aacgccagca ggcgtctctg gaagagcaaa acaacgatgc
     8761 gttaagcccg gcgatccgtc gcctgctggc tgaacacaat ctcgacgcca gcgccattaa
     8821 aggcaccggt gtgggtggtc gtctgactcg tgaagatgtg gaaaaacatc tggcgaaagc
     8881 cccggcgaaa gagtctgctc cggcagcggc tgctccggcg gcgcaaccgg ctctggctgc
     8941 acgtagtgaa aaacgtgtcc cgatgactcg cctgcgtaag cgtgtggcag agcgtctgct
     9001 ggaagcgaaa aactccaccg ccatgctgac cacgttcaac gaagtcaaca tgaagccgat
     9061 tatggatctg cgtaagcagt acggtgaagc gtttgaaaaa cgccacggca tccgtctggg
     9121 ctttatgtcc ttctacgtga aagcggtggt tgaagccctg aaacgttacc cggaagtgaa
     9181 tgcttctatc gacggtgatg acgtggttta ccacaactat ttcgacgtca gcatggcggt
     9241 ttccacgccg cgcggcctgg tgacgccggt actgcgtgat gtcgataccc tcggcatggc
     9301 agacatcgag aagaaaatca aagagctggc agttaaaggc cgtgacggca agctgacggt
     9361 tgaagatctg accggtggta acttcaccat caccaacggt ggtgtgttcg gttccctgat
     9421 gtctacgccg atcatcaacc cgccgcagag cgcaattctg ggtatgcacg ctatcaaaga
     9481 tcgtccgatg gcggtgaatg gtcaggttga gatcctgccg atgatgtacc tggcgctgtc
     9541 ctacgatcac cgtctgatcg atggtcgcga atccgtgggc ttcctggtaa cgatcaaaga
     9601 gttgctggaa gatccgacgc gtctgctgct ggacgtgtag tagtttaagc ttcacctgca
     9661 ctgtagaccg acgtggatag ggtgtcggtc tacggtttaa aagataacga ttactgaagg
     9721 atggacagaa cacatgaact tacatgaata tcaggcaaaa caactttttg cccgctatgg
     9781 cttaccagca ccggtgggtt atgcctgtac tactccgcgc gaagcagaag aagccgcttc
     9841 aaaaatcggt gccggcccgt gggtagtgaa atgtcaggtt cacgctggtg gccgcggtaa
     9901 agcgggcggt gtgaaagttg taaacagcaa agaagacatc cgtgcttttg cagaaaactg
     9961 gctcggcaag cgtctggtaa cgtatcaaac agatgccaat ggccaaccgg ttaaccagat
    10021 tctggttgaa gcagcgaccg atatcgctaa agagctgtat ctcggtgccg ttgtagaccg
    10081 tagttcccgt cgtgtggtct ttatggcctc caccgaaggc ggcgtggaaa tcgaaaaagt
    10141 ggcggaagaa actccgcacc tgatccacaa agttgcgctt gatccgctga ctggcccgat
    10201 gccgtatcag ggacgcgagc tggcgttcaa actgggtctg gaaggtaaac tggttcagca
    10261 gttcaccaaa atcttcatgg gcctggcgac cattttcctg gagcgcgacc tggcgttgat
    10321 cgaaatcaac ccgctggtca tcaccaaaca gggcgatctg atttgcctcg acggcaaact
    10381 gggcgctgac ggcaacgcac tgttccgcca gcctgatctg cgcgaaatgc gtgaccagtc
    10441 gcaggaagat ccgcgtgaag cacaggctgc acagtgggaa ctgaactacg ttgcgctgga
    10501 cggtaacatc ggttgtatgg ttaacggcgc aggtctggcg atgggtacga tggacatcgt
    10561 taaactgcac ggcggcgaac cggctaactt ccttgacgtt ggcggcggcg caaccaaaga
    10621 acgtgtaacc gaagcgttca aaatcatcct ctctgacgac aaagtgaaag ccgttctggt
    10681 taacatcttc ggcggtatcg ttcgttgcga cctgatcgct gacggtatca tcggcgcggt
    10741 agcagaagtg ggtgttaacg taccggtcgt ggtacgtctg gaaggtaaca acgccgaact
    10801 cggcgcgaag aaactggctg acagcggcct gaatattatt gcagcaaaag gtctgacgga
    10861 tgcagctcag caggttgttg ccgcagtgga ggggaaataa tgtccatttt aattgataaa
    10921 aacaccaagg ttatctgcca gggctttact ggtagccagg ggactttcca ctcagaacag
    10981 gccattgcat acggcactaa aatggttggc ggcgtaaccc caggtaaagg cggtactacc
    11041 cacctcggcc tgccggtgtt caacaccgtg cgtgaagctg ttgctgctac tggcgctacc
    11101 gcttctgtta tctacgtacc agcaccgttc tgcaaagact ccattctgga agctattgat
    11161 gcaggcatca aactgattat caccatcact gaaggcatcc cgacgctgga tatgctgact
    11221 gtgaaagtga aactggacga agccggcgtt cgtatgatcg gcccgaactg cccgggcgtt
    11281 atcactccgg gtgaatgcaa aatcggtatc cagccgggtc acattcacaa accgggtaaa
    11341 gtgggtatcg tttcccgttc cggtacactg acctatgaag cggttaaaca gaccacggat
    11401 tacggtttcg gtcagtcgac ctgtgttggt atcggcggtg acccgatccc aggctctaac
    11461 tttatcgaca tcctcgaaat gttcgaaaaa gatccgcaga ccgaagcgat cgtgatgatc
    11521 ggtgagatcg gcggtagcgc tgaagaagaa gcggcagcgt acatcaaaga gcacgttacc
    11581 aagccagttg taggttacat cgctggtgtg actgcgccga aaggcaaacg tatgggccac
    11641 gcgggtgcga tcattgctgg tgggaaaggg actgcggatg agaaattcgc tgctctggaa
    11701 gccgcaggcg tgaaaaccgt tcgcagcctg gcggatatcg gtgaagcact gaaaactgtt
    11761 ctgaaataaa tatctgtaat aagaaatagc cctcgccgct tccctctaca ggaatggcga
    11821 agggctgtcg gtttcgacat ggttggccat cgtatgatgg ccttttttgt gcttatcgcg
    11881 atgattttcg ctgcgctatc agggtaaatt tatagtcatc ggtattaaaa gcgttgcggc
    11941 tatattcaaa cacccgacca tcaactaaat atccacgcga tactttttca agaatcggct
    12001 ttgtctggct gatattaagc agacggctca tctcttcggt tggcatcaga ggaatgattt
    12061 cctgttcgct acgatcgata accatcttct tcacttcttc aataaagtga tatttcgaat
    12121 tttccatgac ctgccaggtg agatccggga acaacgcaag cggcatccag gtttcttcca
    12181 gcgccattgg cttttgcttg cgatagcgca cgcgcttcac atgccacaca cgatcctgcg
    12241 cggtgatttg tagctgttgc tgaagaaaat cgtcagccgg aatcacttca aatatcagga
    12301 tttcactgtg tgtatcgacg tgacggtccg acagtttttc atcaaaactg gttaactgaa
    12361 aaatatcgta attgacccgc tcttctttga cgtaagtccc gctgccctga atgctttcga
    12421 ggatctgctg ctcgactagc tggcgcaaag cctgacgcac cgtaacccgg ctgacgccaa
    12481 actctgtttg tagcgctgat tcagtgggta acgcatcgcc aggtttaagc tcgccacgcg
    12541 caatttgttc acgaatgcga tcggcaatct gccggtataa gggcttgtgt cccattttta
    12601 gtatctcatt aatacgaatt tagccattat gcccgataaa ttcatcctgt aaataataca
    12661 aatacaatac aaataatttc aatcaagtga aattgatcac atgatggtat tgttttatcg
    12721 ggcacactgg cgcgactata aaaacgatca agtgaggatc atgatgaatc tgacgactct
    12781 gacccaccgc gatgcgttgt gtctgaatgc gcgctttacc agccgtgaag aggccatcca
    12841 cgcgttgact caacgtcttg ctgctctggg gaaaatttcc agtactgagc aatttctgga
    12901 agaagtgtat cgccgtgaaa gccttggccc gaccgcctta ggtgaagggt tggctgtgcc
    12961 gcatggcaaa actgctgcgg taaaagaagc ggcgtttgcg gtcgccacac tcagcgagcc
    13021 gcttcagtgg gaaggcgttg atggcccgga agcagttgat ttagtggtgc tgctggcgat
    13081 tccccccaat gaagcgggta ctacgcatat gcaactgctg acagcgctga ccacgcgcct
    13141 tgcggatgat gagattcggg cgcgtataca gtcggcgacg acgcctgatg agttgctctc
    13201 ggcgctggat gacaagggag gcacgcaacc ttctgcctct ttttccaacg cgccaactat
    13261 cgtctgcgta acggcctgtc cggcgggtat tgctcacacc tatatggctg cggaatatct
    13321 ggaaaaagcc ggacgcaaac tcggcgtaaa tgtttacgtt gaaaaacaag gcgctaacgg
    13381 cattgaaggg cgtttaacgg cggatcaact caatagtgca accgcctgta tttttgcggc
    13441 tgaagtcgcc atcaaggaga gtgagcgttt taatggcatt cccgcgcttt cagtgcctgt
    13501 tgccgagccg attcgccatg cagaagcgtt gatccaacaa gcgcttaccc tcaagcgtag
    13561 cgatgagacg cgtaccgtac agcaagatac gcaaccggtg aaaagtgtca aaacggagct
    13621 gaaacaggca ctgttgagcg gaatctcttt tgccgtaccg ttgattgtcg cggggggcac
    13681 ggtgctggcg gtcgcggtat tactgtcgca aatcttcggg ctacaagatc tgtttaatga
    13741 agaaaactcc tggctgtgga tgtaccgcaa gctgggcggc gggctgctcg gaattttgat
    13801 ggtaccggtg ctcgcggcct ataccgccta ttctctggca gataaaccgg cgttagcgcc
    13861 aggctttgcg gctggacttg ccgccaacat gatcggctcc gggtttctcg gcgcggtcgt
    13921 tggcggattg atagccggtt acttgatgcg ctgggtgaaa aatcacttgc gtcttagcag
    13981 taaattcaat ggattcctga ctttttatct ctacccggtg ctcggtacgt tgggagcggg
    14041 cagtctgatg ctgtttgtgg tgggagaacc tgtcgcatgg atcaataact cgcttaccgc
    14101 ctggctgaac ggtctgtcag gaagtaacgc gctgttgctg ggtgccattc tcggttttat
    14161 gtgttccttt gaccttggag ggccagtgaa caaagccgct tatgcattct gcctgggcgc
    14221 aatggcgaac ggcgtttacg gcccgtatgc cattttcgcc tccgtcaaaa tggtttcggc
    14281 atttaccgta accgcttcca cgatgctcgc accgcgcctg tttaaagagt ttgaaattga
    14341 gaccgggaaa tccacctggc tgttagggct ggcaggtatt accgaagggg cgatcccgat
    14401 ggcgattgaa gatccgctgc gggttattgg ttcgtttgtg ctgggctcta tggtaacggg
    14461 cgctattgtc ggtgcgatga atatcggcct ttcgacaccc ggtgccggca ttttctcgct
    14521 ctttttactt catgataatg gcgcgggcgg tgttatggcg gcaattggct ggtttggtgc
    14581 ggcattggtg ggggctgcaa tctcgactgc aattctcctg atctggcggc gtcacgcggt
    14641 taagcatggc aactatctga ctgatggcgt aatgccataa acaaaaagga aacgacgatg
    14701 aaagcggtat ctcgcgttca catcaccccg catatgcatt gggatcggga gtggtatttc
    14761 accaccgaag agtcacgcat tctgctggtc aataatatgg aagagatcct gtgccgactg
    14821 gaacaggaca acgaatacaa atattacgta ctcgacgggc aaacggcgat cctcgaagat
    14881 tatttcgcgg tgaaaccgga aaacaaagac cgcgtgaaga aacaggtaga agccggtaag
    14941 ttgattatcg gcccctggta tacccagacc gataccacga ttgtttctgc ggaatccatc
    15001 gtccgtaatc tgatgtacgg aatgcgtgac tgcctcgcgt ttggcgagcc gatgaaaata
    15061 ggttatttac cggattcctt tggcatgtcc gggcaactgc cgcatattta caatggattt
    15121 ggcattaccc gcactatgtt ctggcgcgga tgttcggagc gccacggtac tgataaaacc
    15181 gagtttttgt ggcaaagcag tgacggtagc gaagtgacgg cgcaggtgct gccgctgggc
    15241 tacgccatcg gtaagtactt acctgccgac gaaaacggat tacgtaaacg cctcgacagt
    15301 tattttgacg tgctggaaaa agcgtctgta accaaagaga ttttgctgcc gaatggtcat
    15361 gaccagatgc cattgcagca aaatatcttc gaagtgatgg ataagctacg tgagatctac
    15421 cctcaacgta agtttgtgat gagccgcttt gaagaggtat ttgagaagat cgaagcgcag
    15481 cgtgaaagtc tggccaccct gaaaggtgaa tttatcgatg gcaaatatat gcgcgtacat
    15541 cgcaccatcg gttccacgcg catggatatc aaaattgccc acgcacgtat tgaaaataag
    15601 attgttaatc tgctggaacc gctggcaacg ctggcctgga cgttgggttt tgaataccac
    15661 cacggcttgc tggagaaaat gtggaaagag atcttaaaaa atcatgccca cgacagtatc
    15721 ggttgttgtt gcagtgacaa agttcatcgc gaaattgtcg cccgcttcga actggctgaa
    15781 gacatggcgg ataatctgct gcgtttctac atgcgcaaaa ttgccgataa catgccacag
    15841 agtgatgccg acaaactcgt gctgtttaac ctgatgccct ggccgcggga agaagttatt
    15901 aacaccactg tgcggctgcg cgccagccag tttaatctac aagacgatcg cggtcagcct
    15961 gtaccgtatt ttattcgcca tgcccgtgag atcgatcctg gcctaatcga tcggcaaatt
    16021 gttcattatg gcaattatga tccctttatg gagtttgata ttcagatcaa ccagattgtc
    16081 ccttctatgg gctatcgcac gctttatatt gaagcgaatc agcctggcaa cgtgattgcg
    16141 gcaaaaagtg acgctgaagg gattctggaa aatgctttct ggcagattgc gctcaatgag
    16201 gatggttctc tgcaactggt agataaagac agcggtgtgc gctatgaccg ggtattgcaa
    16261 attgaagaaa gctctgatga tggtgatgaa tatgactatt cacccgcaaa agaagagtgg
    16321 gtaattaccg cagcgaacgc gaaaccgcaa tgcgatatta ttcatgaagc ctggcagagc
    16381 agggctgtta tccgctatga catggcagtg ccgctcaatt tgtcagaacg cagcgcccgg
    16441 caatccactg gcagagtagg gggggtgttg gttgtcactc ttagtcataa cagcaggcgt
    16501 attgatgtgg atatcaacct tgataaccag gctgacgatc atcgccttcg tgtcctggtc
    16561 cctacacctt ttaacactga cagtgttctg gcagatacgc agtttggttc gctaacgcgc
    16621 cccgtgaacg acagtgcaat gaacaactgg cagcaagaag gctggaaaga agcgccggtt
    16681 ccggtatgga atatgctcaa ctatgttgcc ttacaggaag ggcgtaacgg catggctgtc
    16741 tttagcgaag ggttacgtga atttgaagtc atcggtgaag agaagaaaac ctttgccatt
    16801 acgttgctgc gtggcgtggg cttactgggc aaagaagatc tgcttttaag gcctgggcgg
    16861 ccttcgggaa ttaaaatgcc agtcccggac tcacaactac gtggtctgct ttcttgtcgc
    16921 ctaagtttat tgagttatac cggtacgcca accgccgctg gtgtagctca gcaggcgcga
    16981 gcatggctga ctccagtaca gtgttacaac aaaatcccat gggatgcgat gaagctcaac
    17041 aaagccggat tcaacgtgcc ggaaagttat agtttgttga aaatgccccc agtgggatgc
    17101 ctgataagcg cacttaagaa agctgaagac cgacaagaag tgattttacg gctgtttaat
    17161 ccggctgaat cagcaacctg tgatgcgact gttgctttca gtcgcgaggt gatttcttgc
    17221 tcagaaacga tgatggatga acacattacc accgaggaaa atcaaggttc aaatctatcg
    17281 gggccttttt tacccggcca gtcacggacg ttcagttacc ggcttgcctg aatagcaatc
    17341 aaaccgaagc cacatatgcg cggccagatt gttgacaaag ggcgctttgt tcatgccgga
    17401 tacggcatga acgctttatt cggtctacaa aagcaggcaa attcaatata ttgcagagat
    17461 gatgtagaca ctggcaagcg tagcgcatca ggtaattttg cgtttatctt cactctcaag
    17521 ccacgtatat gtggctttat ttttaacaaa ataataaccc tgggtgagtt aattataata
    17581 taattacaag ttaattaaat gttaatatcg gcggggtgga tttatgcctt tattagtaat
    17641 cctgaaactc tgcgtcgtat tagccagtga ccaaaaaaag aattaaggtc aaccgtgctg
    17701 tttttgcttc gtctcttttt atctttaaat tgccaaccga aactaatttc agccttataa
    17761 ctcacacatt ttaaacataa atgtcactaa agttacctta ttgaaacatg attaacataa
    17821 t
//
LOCUS       DAZPEP010000077        17809 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZPEP010000077 DAZPEP010000000
VERSION     DAZPEP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17809
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.077"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            94..772
                     /locus_tag="ACHUT9_002537"
                     /pseudo
     CDS             94..772
                     /locus_tag="ACHUT9_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            816..5666
                     /locus_tag="ACHUT9_002538"
                     /pseudo
     CDS             816..5666
                     /locus_tag="ACHUT9_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6034..7689
                     /gene="lldP"
                     /locus_tag="ACHUT9_002539"
     CDS             6034..7689
                     /gene="lldP"
                     /locus_tag="ACHUT9_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFR1348984.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7689..8465
                     /gene="lldR"
                     /locus_tag="ACHUT9_002540"
     CDS             7689..8465
                     /gene="lldR"
                     /locus_tag="ACHUT9_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFR1348985.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8462..9652
                     /gene="lldD"
                     /locus_tag="ACHUT9_002541"
     CDS             8462..9652
                     /gene="lldD"
                     /locus_tag="ACHUT9_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFR1348986.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9836..10309
                     /gene="trmL"
                     /locus_tag="ACHUT9_002542"
     CDS             9836..10309
                     /gene="trmL"
                     /locus_tag="ACHUT9_002542"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFR1348987.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10362..11183)
                     /gene="cysE"
                     /locus_tag="ACHUT9_002543"
     CDS             complement(10362..11183)
                     /gene="cysE"
                     /locus_tag="ACHUT9_002543"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFR1348988.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11263..12282)
                     /gene="gpsA"
                     /locus_tag="ACHUT9_002544"
     CDS             complement(11263..12282)
                     /gene="gpsA"
                     /locus_tag="ACHUT9_002544"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFR1348989.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12282..12749)
                     /gene="secB"
                     /locus_tag="ACHUT9_002545"
     CDS             complement(12282..12749)
                     /gene="secB"
                     /locus_tag="ACHUT9_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFR1348990.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12812..13063)
                     /gene="grxC"
                     /locus_tag="ACHUT9_002546"
     CDS             complement(12812..13063)
                     /gene="grxC"
                     /locus_tag="ACHUT9_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFR1348991.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13205..13636)
                     /locus_tag="ACHUT9_002547"
     CDS             complement(13205..13636)
                     /locus_tag="ACHUT9_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFR1348992.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13881..15425
                     /gene="gpmM"
                     /locus_tag="ACHUT9_002548"
                     /gene_synonym="pgmI"
     CDS             13881..15425
                     /gene="gpmM"
                     /locus_tag="ACHUT9_002548"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFR1348993.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15459..16718
                     /gene="envC"
                     /locus_tag="ACHUT9_002549"
     CDS             15459..16718
                     /gene="envC"
                     /locus_tag="ACHUT9_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFR1348994.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16722..17681
                     /locus_tag="ACHUT9_002550"
                     /pseudo
     CDS             16722..17681
                     /locus_tag="ACHUT9_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
     gene            complement(17668..>17809)
                     /locus_tag="ACHUT9_002551"
     CDS             complement(17668..>17809)
                     /locus_tag="ACHUT9_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1348995.1"
                     /translation="MYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHHRITRSAFN
                     LR"
BASE COUNT         4458 a         4612 c         4668 g         4071 t
ORIGIN      
        1 ctgtcattaa tgtaaagcaa catagatcac caataataca atggacaact ctaccggcat
       61 tgatcgctgg tcggaggaaa taaggatttt tacatgaact tgaagaagat ttttttcagt
      121 gctgtcactg tttcagttct ctgcgcatta acgggatgtg actacattga agaaggcaag
      181 ccagaaagta gtttattgaa acaacaagaa gaacattaaa attgatctac ttgaaaaaca
      241 acaagcacag ctaaagagtc aactggaaac tattcaaaaa caacaaactg gcataataag
      301 cagtacaaag actttaactc acgtaattaa atccgttaaa gatcaacaaa acacttttat
      361 ttttaccgag tttaacccag caaaaacgaa gtatttcatc ctgaacaatg gttcggtggc
      421 tttagcgggt cgagtgttat ccattgacgc cacagagaat ggcagtgtta ttcatatttc
      481 actggtcaac ttattaagta cacctatctc aaatattggt tttaatgcga catggggtgg
      541 tgaaaaacct gtcgatgcca aagagtttgc ccggtggcaa caattacttt tcaacacatc
      601 aatgaaatcc acattgaaat tattaccagg tcaatggcaa gacattaatt tgaccctgaa
      661 gggtgtatcg cccaataacc tgggatatct gaagttagcc atcaacatgg aaaatattca
      721 gttcgacaat cttccctctg ctgaaaatcg gcagaaaaga agcaaaaaat aaatgcagtt
      781 agtacgctta taacaaggaa tagcgagcat cattaatgaa caaaatattt aaagttatct
      841 ggaaccctgc gacagggaat tatactgtta ccagcgaaac ggcaaaaagc cgtggcaaga
      901 aatctgggcg cagtaagctg ttaatttctg cgctggttgc gggtggaatg ttgtcgtcgt
      961 ttggggcatt ggcgaatgcc gggaatgaca acggccaggg tgttgattac ggtagtggat
     1021 cagctggcga cagctgggtt gctataggca aaggggcgaa agcaaatact tttatgaaca
     1081 ccagtggttc cagtactgct gtgggttatg acgctatagc tgaaggccaa tatagctctg
     1141 ccatcgggtc aaaaacccat gcgattggtg gtgcatcaat ggcctttggg gttagtgcaa
     1201 tatcagaagg cgatagaagt atagcactgg gtgcctcttc gtattcattg ggccaatact
     1261 caatggccct cggccgttat tcaaaagcat tgggtaaatt gtctattgct atgggggact
     1321 cttccaaagc ggaaggagca aacgccattg ccctgggaaa tgccactaaa gctactgaga
     1381 ttatgagtat tgctcttggc gacaccgcca atgcgtcaaa agcgtattca atggcgctgg
     1441 gagcaagtag cgtcgcatct gaagaaaacg caattgccct ggggcgtagc agtgtagcta
     1501 gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg aacgctattg
     1561 cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat aatgcgaagg
     1621 caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa gtcaatacta
     1681 tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata ggctagggca
     1741 acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg agaggcttaa
     1801 acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt gcgcttggta
     1861 gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc attgcggatt
     1921 tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt aatgtgaaaa
     1981 atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacagctt tatgccatta
     2041 gcgactcggt agcaaaaacg cttggaggag gggctgcagt agatgttgat gacggtactg
     2101 ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg gctgcgctcg
     2161 ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac agcgctgctc
     2221 atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc acgatttcag
     2281 cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac gatgtcgaag
     2341 ccaacaccgc caatatcgct actaatacca gcaacattgc cacgaataca gcaagtattg
     2401 ccaccaatac caccaatatc accaacctga cggattccgt tggtgacctt caggctgatg
     2461 ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag gataccacca
     2521 gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat gctgttaacg
     2581 gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat atcgccaata
     2641 acacttccaa tattgccact aacaccacca acatctctaa tctgactgag acggtgacta
     2701 atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg gcagctcatg
     2761 gcaccgagac caccagcaaa atcaccaacg ttaaagatgg cgacctgacg actggcagca
     2821 ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg acgaatacca
     2881 ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg actaatcttg
     2941 gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct catggcaaca
     3001 ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc agttccgatg
     3061 ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac ttcggcggca
     3121 atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc ggtgaaacaa
     3181 attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc acgtctctcg
     3241 gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac ggtactaatg
     3301 gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc agttctgacg
     3361 cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg ctggggggtg
     3421 gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt gctaatgctg
     3481 attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcttgac gacgctctgc
     3541 tctgggatga ggacgccggt gaaaatggtg catttagcgc cgctcacgga aaagataaaa
     3601 ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc agcgacgcga
     3661 ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg ggtggtgacg
     3721 cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg aacgccgagt
     3781 acaacaacgt cggtgacgcc ctggatgcgc ttgatgataa cgccctgctg tgggatgaga
     3841 ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc agcatcatca
     3901 ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac ggttctcagt
     3961 tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag ctcgctggta
     4021 acaccgacgc aacctatatc gaagaaaatg gcgctggtat caactacgtt cgtactaacg
     4081 acaacgattt agcctttaac gatgcaagcg cctctggtgt cggcgctaca gctgtaggtt
     4141 ataacgctgt cgcgtctggt gccagcagcg tagccattgg tcagaacagc agcagcaccg
     4201 ttgataccgg tattgcgctg ggtagcagct ccgtttccag ccgtgtgatt gccaaaggtt
     4261 ctcgtgacac tagcgtaacg gaaaatggcg tggtgattgg ttatgacacc actgacggcg
     4321 aactgctagg tgcattgtca attggtgatg acggtaaata ccgccaaatc atcaacgtag
     4381 ctgatggttc agaagcccat gacgccgtta cggttcgcca gttgcagaac gctattggag
     4441 cggtcgccac tacgccaacc aagtactttc acgccaactc aacggcagaa gactcactgg
     4501 ccgttggtga agactcgctg gcaatgggtg cgaaaactgt cgttaatggt aatgcaggta
     4561 ttggtatcgg tttgaacact ctggttctag ctgatgcgat caacggcatt gctatcggca
     4621 gtaacgcacg cgcaaaccat gcaaacagta tcgcaatggg taacggttct cagaccaccc
     4681 gtggtgcaca gactggctac accgcctaca acatggacgc accgcagaac tctgtaggtg
     4741 agttttctgt cggtagtgaa gacggtcaac gtcagatcac aaacgtcgca gctggttcag
     4801 cggataccga tgcggttaac gtgggtcagt tgaaagtcac tgatgagcgc gtagcgcaaa
     4861 atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact cgcgttacta
     4921 atatcgaaaa cggtattggc gacattgtca ccaccggtag caccaagtac ttcaagacca
     4981 acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt ggttctggtt
     5041 ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca gaggaagaaa
     5101 atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta gctgccggtg
     5161 ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca ggcggcgtgc
     5221 gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc ggtggtggca
     5281 acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac gacgcggtga
     5341 actacgcgca gttgaagcaa agcgtgcagg aaacgaagca atacaccgat cagcggatgg
     5401 ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt atcgcttctg
     5461 caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg gcttctattg
     5521 gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg gtgagcgcca
     5581 atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa tactccgccg
     5641 cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta agggcaaacg
     5701 ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgccccggg tcaacaatct
     5761 aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc cccacaagaa
     5821 ttggccctac caattcttcg cttatctgac ctctggttca caattcccca attaaaactc
     5881 acatcaatat tgccaataca taacatttag ttaaccattc attgtcatta tccctacaca
     5941 acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca acagactcat
     6001 tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca aaactacgat
     6061 cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct gtttttcttc
     6121 tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac ggtagcaatc
     6181 gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct ggcctcggtg
     6241 gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc agcagtgttc
     6301 gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc tattctttcg
     6361 ataacccctg accagcgtct gcaaatgctg atcgtcggtt tctgtttcgg cgctttcctt
     6421 gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct ggtcggcctg
     6481 ggttttaaac cgctgtacgc cgccggactg tgcctgattg ttaacaccgc gccagtggca
     6541 tttggcgcga tgggcattcc aatcctggtt gccggacaag taacaggcat cgacagcttt
     6601 gagattggcc agatggtggg gcggcagcta ccgtttatga ccattatcgt gctgttctgg
     6661 atcatggcga ttatggacgg ctggcgcggt atcaaggaga cgtggcctgc ggtcgtggtt
     6721 gcgggcggct cgtttgccat cgctcagtac cttagctcta acttcattgg gccggagctg
     6781 ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct caaacgctgg
     6841 cagccagtgc gcgtattccg ttttggtgat ttgggggcgt caaagattga tatgacgctg
     6901 gcccacaccg gttacactgc gggtcaggtg ttacgtgcct ggacaccgtt cctgttcctg
     6961 acagctaccg taacactgtg gagcatcccg ccgtttaaag ccctgttcgc atcgggtggt
     7021 gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcgataaact ggttgcccgt
     7081 atgccaccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt tgactggttc
     7141 tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg gctgaagatg
     7201 aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc tctacccatc
     7261 tactccatcg gcatggtgct ggcgttcgcc tttatctcga actattccgg actgtcatca
     7321 acactggcac tggcactggc gcacaccggt catgcattca ccttcttctc gccgttcctc
     7381 ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct gttcgccgcg
     7441 ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt tgccgccaat
     7501 accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat cgcctgtgcg
     7561 gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa acacagcctg
     7621 atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt aacgtggatg
     7681 attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat cgtgtgcggg
     7741 cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgag cgccaactgg
     7801 cgatgcaact cggcgtatca cgtaattcac tgcgcgaggc gctggcaaaa ctggtgagcg
     7861 aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt catgacacat
     7921 ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat ccggattaca
     7981 gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg catgcggcaa
     8041 tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca acgctaagtg
     8101 aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt gccgaagcct
     8161 cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg caatcctcag
     8221 tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa ctgaccgaac
     8281 aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg cgtaaagcaa
     8341 tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa gatcaggctc
     8401 gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg gagaaaaacg
     8461 catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc tgccgccgtt
     8521 cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc gcaacgtgga
     8581 agatttgtca gaagtggcac tgcgccagcg tattctgaaa aacatgtccg acttaagcct
     8641 ggaaacgacg ctgtttaatg agaaattgtc gatgccggta gcactggcac cggtgggttt
     8701 gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg acgcgcatgg
     8761 cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg cgccagccat
     8821 caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta tgcgtaacgc
     8881 gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg atatgccgac
     8941 accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg cggcaatgcg
     9001 ccgctacttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc tgaacggtcg
     9061 tccacatgat ttaggtaata tctcagctta tctcggcaaa ccgaccggac tggaagatta
     9121 catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc ttgagtggat
     9181 ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg aagatgcgcg
     9241 cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg gccgccagct
     9301 ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt gcagatgcgg tgaaaggtga
     9361 tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc gtatgattgc
     9421 gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg caacagcggg
     9481 ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag tggcgatgac
     9541 gctgactggc gcgaaatcga tcagtgaaat tacgcaagat tcgctggtgc aggggctggg
     9601 taaagagttg cctacggcac tggctccgat ggcgaaaggg aatgcggcat agtcgtttgc
     9661 gccccctcac cctcagggag aggggaccgt tcggcgctgt atgtactccc tcactctgaa
     9721 acgacaccgc actctttttt tctccctcgc ccctccgggg agggccgggg tgaggggaaa
     9781 aggccgcacg gttcaccaat tttctgctat cctgcccccg cactaagggg gcagtatgct
     9841 aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaacatca tccgtctttg
     9901 cgctaatacc ggctttcgtc tgcatatcat cgaaccgatg ggatttgcct gggacgataa
     9961 gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc gtcatcatga
    10021 ctatcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca ccacgaaagg
    10081 tacgcctgct cacagcgccg taagctatca ggatggcgat tatctgatgt tcggcccgga
    10141 aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa aaattcgcat
    10201 tccgatggtg ccggacagcc gcagcatgaa tctgtccaat gcggtgtcgg tagtggtgta
    10261 tgaagcctgg cggcagctgg gatatccggg agcgttgttg agagattagt tactgtatgc
    10321 cggatagatg attacatcgc atccggcacg atcacaggac attagatccc atccccatac
    10381 tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg cttatcgctg
    10441 tctggtttac cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt
    10501 tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat attgccgagg
    10561 attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg gtcaccacca
    10621 gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc
    10681 gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg gccaattttt
    10741 gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag aaagattgcc
    10801 aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg caaggcatga
    10861 aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc gcgggtacgc
    10921 accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc gtaggcttct
    10981 tccaccactt cacggatagc aatagcaggc ataattggcg atgacagctt gttcgccagc
    11041 atgtagctca gtgcactacc aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg
    11101 gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt gttccagaca
    11161 atttccagtt cttcacacga cattgcttac tccacacgat gagataatga ccggcccgtt
    11221 ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtggc tgctgcgctc
    11281 gtccttgcgt gcacgaccta ataacgtcaa tgctgcctcg cgcgcgtttt ttccgcaata
    11341 taatacttga taaatttcct cggttattgg catttctacg ccgaagcgat gcgccagttc
    11401 gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct cctgcgcgct
    11461 ttgtacatcc atgccctgac cgagcatcat gccaaaacgg cggttacgcg actggttgtc
    11521 ggtacaggta agcaccagat cgcccagccc cgccatgccc ataaaggtgg caggatcggc
    11581 acccagcgcc gcgccaagac gcgacatttc agccagccca cgggtgatca gtgccgtacg
    11641 cgcattcgca ccaaaaccga taccgtcgga catccccgcg ccaatggcaa tgacgttttt
    11701 caccgcaccg ccaagctgca caccgatgaa atccgggttg ctgtaaacgc ggaaactttt
    11761 gccacagtgc agcaattgtt ggagatcatc ggcaaaggtc tggtcggtag aggccaacga
    11821 aatagccgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag agataaccgc
    11881 cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac gtccggtttc
    11941 cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca gcggtttaat
    12001 ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat tacggctggc
    12061 tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag gcacatcggg
    12121 gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc
    12181 ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg tgccgtacga
    12241 gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc ctgatgttct
    12301 tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc gaagttaatc
    12361 ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca cgctggtgat gcactcacga
    12421 gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct
    12481 tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa cgcggtttct
    12541 tcgcccaggg aggccgttac ggtaacacgc agtaccactt cgtatacgtc atctgccagt
    12601 tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt ctggaaaacg
    12661 tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg gatctggaaa
    12721 gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa
    12781 tactgcgcaa tgtaatgcca acgttcacac gttatttcag caggggatcc agtccaccac
    12841 gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct
    12901 ggggaaccgt ggtgcgaccg ctgcgtttga tcatctcttc acgcttggcg gcattgccat
    12961 cgatcggcaa ctcctgaaaa ctcacgccct tgctgctcag cagtgctttt gcacgatggc
    13021 aatacgggca ggtttctttg gtatagattt caacattggc cataatttag ctcctgtttt
    13081 tttaccctga aatttcatgt tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat
    13141 aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa agacgacagg
    13201 taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca acgccctctt
    13261 tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc tcctggcact
    13321 gcatgccaga accgtctacc acgataaccg gtttgtcttt gtgcttctca agctcaccaa
    13381 cattgttggc tttgatttcg ctcggcaaca gattgataga acctgcgata tggcctttac
    13441 ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg atcagacgcg
    13501 tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac gtagtcacaa
    13561 gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg ccaacaaatt
    13621 gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa
    13681 ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat gcaattttgc
    13741 ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt
    13801 ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa attttgactc
    13861 ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg gtgattctgg
    13921 atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct aaaaccccgg
    13981 taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc ggtctggaag
    14041 tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac ctgggtgccg
    14101 gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat cgcgctttct
    14161 ttgctaatcc agtgctgact ggcgcggtag ataaagcgaa aaacgcaggt aaagcagtac
    14221 acattatggg tctgctctcc gcaggcggcg tacacagcca cgaagatcac atcatggcga
    14281 tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg
    14341 gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg
    14401 ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg atggaccgcg
    14461 ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg cagggtgagt
    14521 ttcaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac gaaaacgacg
    14581 agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca atggaagacg
    14641 gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc actcgtgcat
    14701 tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga
    14761 tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg
    14821 ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa
    14881 ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag tcgttcaaag
    14941 gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg caaccggaaa
    15001 tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc aaatacgaca
    15061 ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg atggaagcgg
    15121 cggttaaagc ggttgaagca ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt
    15181 ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag atgcgcgatc
    15241 cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg atttacgttg
    15301 gtgataagaa cgtgaaagcg gttgcaggcg gcaaactttc tgacatcgcg ccgaccatgt
    15361 tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg ttcatcgtgg
    15421 aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc
    15481 gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc gtattgttgt
    15541 gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag gccgatatcg
    15601 ccacaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat
    15661 tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca
    15721 cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc aaactggagc
    15781 agcaaaaagc cgcccaggag cgcagcctcg ccgcacaact ggatgccgca ttccgtcagg
    15841 gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt ggacagcgtt
    15901 tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc
    15961 aaacgcgtga agaagtcgcc atgcagcgtg ctgaactgga agagaaacaa agcgagcaac
    16021 aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggct ctgaacgagc
    16081 gtaaaaagac gctggcgggg ctggaatctt ccatccagca aggtcagcag cagctaagtg
    16141 aactgcgtgc caacgaatcc cgtctgcgta acagtattgc ccgtgcggaa gccgcggcga
    16201 aagcgcgtgc tgaacgagaa gcgcgtgagg ctcaggcagt tcgcgacagg cagaaagaag
    16261 cgacgcgcaa aggcaccacc tacaaaccga ccgaaagcga aaaatcgctg atgtcccgaa
    16321 ctggtggcct gggtgcgccg cgtggtcagg cattctggcc agttcgtggc cctacgctgc
    16381 atcgctatgg cgagcagcta cagggtgaat tacgctggaa aggaatggtt atcggtgcct
    16441 ctgaaggtac tgaagttaaa gcaattgccg acggtcgggt gattctggct gactggctgc
    16501 aaggctacgg tctggtggtg gtggttgagc acggtaaagg cgatatgagt ctttacggct
    16561 ataatcaaag cgcgctggtg agcgttggtt cgcaggttcg tgcgggccag ccaattgcac
    16621 tggtgggcag cagtggcggt cagggcagac cttcactcta tttcgaaatt cgccgccagg
    16681 gtcaggccgt caatccacag ccgtggttgg gaagataagt tttgtttcca tttcgtcgta
    16741 acgttcttgc atttgccgct ctgttggcgc tctcctcccc tgtaattgct ggcaaacttg
    16801 ccatcgtcat tgatgatttt gggtatcgcc cgaacaacga aaaccaggtg ctggcgatgc
    16861 cttccgctat ctccgtcgct gtattacccg attcaccgca cgcctgagag atggcgacca
    16921 aagcgcataa cagcggtcac gaagtgttga ttcatctccc gatggcaccg ttgagtaaac
    16981 agccgctgga gaaaaatacg ctgcgcccgg agatgagcag cgacgaaatt gagcgcatta
    17041 ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caataaccac atgggcagca
    17101 agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcgctggag cgttataatc
    17161 tttacttcct cgacagcgta accattggta atacccaggc gatgcgcgcc gcgcaaggca
    17221 ctggcgtgaa ggtgatcaaa cggaaggtgt tcctcgacga ttcacagaac gaagcggaca
    17281 tccgtgtgca attcaatcgc gcaattgacc tagcgcgtcg caacggatca accattgcca
    17341 ttgggcatcc tcacccttca acggtacgcg tgttgcaaca gatggtttat aacctgccgc
    17401 cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt gatacgtcta
    17461 caccgccgaa aaacactgtg cccgacacac cgcgtaatcc gttccgtggc gtgaagctgt
    17521 gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa gtattaagcg
    17581 aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa ggctggggca
    17641 aacagcctga agcggcgaag cttaacgcta gcgtaaatta aaggcgctac gcgtaatgcg
    17701 atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca gtaacgcctg
    17761 ataacccacc tttacgctgc gcacattggt aatcaggcgc ttatacata
//
LOCUS       DAZPEP010000078        17459 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZPEP010000078 DAZPEP010000000
VERSION     DAZPEP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17459
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.078"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..501)
                     /locus_tag="ACHUT9_002552"
     CDS             complement(<1..501)
                     /locus_tag="ACHUT9_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1348996.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQR"
     gene            complement(687..771)
                     /locus_tag="ACHUT9_002553"
     tRNA            complement(687..771)
                     /locus_tag="ACHUT9_002553"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(735..737),aa:Leu,seq:caa)
     gene            967..1986
                     /gene="ahr"
                     /locus_tag="ACHUT9_002554"
     CDS             967..1986
                     /gene="ahr"
                     /locus_tag="ACHUT9_002554"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFR1348997.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHUT9_002555"
     CDS             complement(1990..2553)
                     /gene="idnK"
                     /locus_tag="ACHUT9_002555"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1348998.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2770..3801
                     /gene="idnD"
                     /locus_tag="ACHUT9_002556"
     CDS             2770..3801
                     /gene="idnD"
                     /locus_tag="ACHUT9_002556"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFR1348999.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3825..4589
                     /gene="idnO"
                     /locus_tag="ACHUT9_002557"
     CDS             3825..4589
                     /gene="idnO"
                     /locus_tag="ACHUT9_002557"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFR1349000.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4666..5985
                     /gene="idnT"
                     /locus_tag="ACHUT9_002558"
     CDS             4666..5985
                     /gene="idnT"
                     /locus_tag="ACHUT9_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFR1349001.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6052..7050
                     /gene="idnR"
                     /locus_tag="ACHUT9_002559"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6052..7050
                     /gene="idnR"
                     /locus_tag="ACHUT9_002559"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFR1349002.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7128..8630
                     /locus_tag="ACHUT9_002560"
     CDS             7128..8630
                     /locus_tag="ACHUT9_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFR1349003.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8749..9831)
                     /gene="lptG"
                     /locus_tag="ACHUT9_002561"
     CDS             complement(8749..9831)
                     /gene="lptG"
                     /locus_tag="ACHUT9_002561"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFR1349004.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9831..10931)
                     /gene="lptF"
                     /locus_tag="ACHUT9_002562"
     CDS             complement(9831..10931)
                     /gene="lptF"
                     /locus_tag="ACHUT9_002562"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFR1349005.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            complement(10994..11044)
                     /gene="ytgA"
                     /locus_tag="ACHUT9_002563"
     CDS             complement(10994..11044)
                     /gene="ytgA"
                     /locus_tag="ACHUT9_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFR1349006.1"
                     /translation="MSIIILATISTTQENK"
     gene            11198..12709
                     /gene="pepA"
                     /locus_tag="ACHUT9_002564"
     CDS             11198..12709
                     /gene="pepA"
                     /locus_tag="ACHUT9_002564"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFR1349007.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12843..13286
                     /gene="holC"
                     /locus_tag="ACHUT9_002565"
     CDS             12843..13286
                     /gene="holC"
                     /locus_tag="ACHUT9_002565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFR1349008.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13286..16141
                     /gene="valS"
                     /locus_tag="ACHUT9_002566"
     CDS             13286..16141
                     /gene="valS"
                     /locus_tag="ACHUT9_002566"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFR1349009.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16197..17393)
                     /locus_tag="ACHUT9_002567"
     CDS             complement(16197..17393)
                     /locus_tag="ACHUT9_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFR1349010.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLTLLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4486 a         4343 c         4454 g         4176 t
ORIGIN      
        1 gcgctgttga agacgagcag ccacttcaag acgtccagac atttctacgg ccttaatagg
       61 tgctaataaa tctcgagttt ttaactcagc gatattacgt tcaccaagcg ctgcaaaaag
      121 attatcttcc agacttttta gcacacgatg ggcatgatct tcagaccact ttttattggt
      181 gccatgccac tcaatcgcga cttctttaaa ggttcgtgct ttactctgtt caaccttatc
      241 acttttcttt ttgtctcccg gatcgacgcc attcgcaagc agcttacgtg gctcgtcacg
      301 acgtattctg gcatccgcga gtgtgatttc aggataaacc ccaagtgcca gcatcttttg
      361 ctttccctca taacggtact gcaaacgcca gtacttagaa ccatttggat ggacaagcag
      421 atgcataccg ccaccatcag tcaacttata ttgcttatct gttggcttag ctgttctgac
      481 tttgatgtct gttaatgcca tgcgtattcc ctccctgttg gtacaagcat tatcgaaccg
      541 gaaataccat catctgtacc aacatatgtt ggtagaagta cgttgatatc ggttgacctt
      601 gagagagtta atatagcggg aaaggtagat aaatactgaa tttcaggcat aaaaaagacc
      661 tcagttgagg tctattgaca tacttttggt gccgaaggcc ggactcgaac cggcacgtat
      721 ttctacggtt gattttgaat caactgcgtc taccgatttc gccacttcgg cactgaagag
      781 gtatgcggaa aacgttgtgg attatacctg tcgcacgcca ccatgcaagc gccagcttgc
      841 tcgcacctcg ctaagtgctg aaattttcag cattgcatac agcgatgtgt aacctttgtc
      901 acactccagg caccccgccc tgccatgctc tacacttccc agacaacacc agagaaggac
      961 caaaaaatgt cgatgataaa aagctatgcc gcaaaagaag cgggcggcga actggaagtt
     1021 tatgagtacg agcccggtga attgaagcca caagatgttg aagtgcaggt ggattactgc
     1081 gggatctgcc attccgatct gtcgatgatc gacaacgaat ggggattttc acagtatccg
     1141 ctggttgccg ggcatgaggt gattgggcgc gtggtggcgc tcgggagcgc cgcgcaggat
     1201 aaaggtttgc aggtcggtca gcgtgtcggg attggctgga cggcgcgtag ctgcgggcat
     1261 tgcgacgcct gtattagcgg taatcagatc aactgcgagc aaggtgcggt gccaacaatt
     1321 atgaatcgcg gcggttttgc cgagaagttg cgtgcagact ggcaatgggt gatcccactg
     1381 ccggaaaata tcgatatcga gtccgccggg ccgctgctgt gtggtggtat cacggtcttt
     1441 aaaccactgt tgatgcacca tatcactgct accagccgcg ttggggtaat tggtattggc
     1501 gggctggggc atatcgctat aaaacttctg cacgcaatgg gatgcgaggt gacagccttt
     1561 agttcgaatc ctgcgaaaga gcaggaagta ctggcgatgg gtgccgataa agtggtgaat
     1621 agccgcgatc cgcaggcact gaaaacactt gcggggcagt tcgatctcat tatcaacacc
     1681 gtcaacgtca gcctcgactg gcagccttat tttgaggcgc tgacgtatgg cggtaatttc
     1741 cacaccgtcg gtgcggtcct cacgccgctg tctgttccgg cctttacgtt aattgcgggc
     1801 gaccgcagcg tctctggttc agcaaccggc acaccttatg agcttcgtaa gctgatgcgc
     1861 tttgcggccc gcagcaaggt tgcgccgacc accgaactgt tcccgatgtc gaaaataaac
     1921 gacgccatcc agcatgtgcg caacggtaag gcgcgttacc gcgtggttct gaaagccgat
     1981 ttttgaaaat cattcgcagc gctgatctga ggcgctaccc cctttcgcac atattctgtc
     2041 ttgtcgtatc gccagcacgg cctgccgaca ttgttcggtg acattggcaa tatcatggtt
     2101 gatatcaatg cgtacaatat cctgttcatc tgcttgtgga cgctccagcg cctcaaactg
     2161 actttttagt aacgctaccg gcataaaatg cccagcccgc cgctgcattc gcgcgagaat
     2221 agtttcatag tcgccatcta accagaggaa atgaacatgg gggctaccct tgcgtaaaat
     2281 atcacgatac tgttttttta atgatgaaca gacaataaat cctgtttcat tctttttata
     2341 aagactgtat gaagcatcat ttaagcgttc cagccaggga agtcgatctt catcagataa
     2401 tggaataccc tgcgacattt tatctatatt tttggctgga tgaagatcgt caccatcaat
     2461 aaatttagca gataataacg cggcaacctt gctaccaatt aatgttttac cactccctga
     2521 aacgcccatc aaaataaagc tttcacccgc catttaaaat ctcctgaatc atcttacctg
     2581 ctgcgatact acgcctggtg gcataataat ttcacgtttt tttatttctg ctgataagaa
     2641 ttacaaggca catcacgtta tgcgtaacat agtaatgtaa caattttctg acgtgatctt
     2701 catcacaaat aatgacaatt aaaaccgctt aaatgcttcc gaggtgtcta cctgaccagt
     2761 gaggaattta tgcaagtgaa aacacagtcc tgcgttgttg cggacaagaa aactgttgcc
     2821 gttaccgagc agacgataga ttggaataat aatggaacat tagtacaaat aacccgaggt
     2881 ggaatttgcg gttccgattt acattattat caggaaggaa aagtaggtaa tttcatgata
     2941 aaggcaccga tggtgttagg tcatgaagtt atcggtaaag ttattcatag cgactcatca
     3001 gaattacatg aagggcaaac ggtagccatt aatccgtcta aaccgtgcgg tcactgcaaa
     3061 tactgcattg aacataacga gaatcagtgt acagatatgc gtttttttgg cagtgccatg
     3121 tatttccctc atgttgatgg tggttttacc cgttataaaa tggtcgaaac gtcgcaatgt
     3181 gtcccttatc cggccaaagc tgacgaaaag gttatggctt ttgccgaacc tttagccgtc
     3241 gcgattcatg ccgcacatca ggccgacgag ttacagggca agcgagtatt tgtttccggt
     3301 gttgggccca ttggctgcct gattgtcagt gctgtgaaaa cactggggtc cgcggaaatt
     3361 gtctgtgccg atgtgagtcc ccgttccctt tcgctggcca aagagatggg tgcggatgtg
     3421 ctcgtaaacc cacaaaacga cgacatggat cactggaaag cggaaaaagg ccatttcgat
     3481 gtcagctttg aagtgtccgg tcatccttca tcagtgaata cctgtctgga ggtcactcgt
     3541 gcacgcggcg taatggtgca ggtagggatg ggaggcgcga tggcagaatt cccaatgatg
     3601 acgttgattg gtaaggagat ttcactcaaa ggctctttcc gttttaccag cgaatttaat
     3661 accgcagtgt catggctggc gaatggcgtt atcaatccac tgcctttact gagtgctgaa
     3721 tatctcttca ctgacctgga agaggcgcta cgtttcgccg gtgataaaac ccaggcagca
     3781 aaagtccagc ttgttttcta agagataaat aaaggaataa tacaatgaac gatctatttt
     3841 cactggcagg aaaaaatatc ttgattaccg gttcagcaca gggcattggc tatttactgg
     3901 caaccggcct gggtaaatat ggcgcacaaa taattattaa tgatattact gccgaacgcg
     3961 cagaacttgc tgtagaaaaa ctccaccagg agggtattca ggccgttgcc gcacctttta
     4021 atgttactca taaacatgaa attgatgccg ccgttgaaca tatcgaaaag gacatcggcc
     4081 ccattgatgt gctggtgaat aacgccggta tccagcgccg tcatcctttt actgagttcc
     4141 ctgaacaaga gtggaatgat gtgatcgcag taaaccagac cgccgtgttc ctggtatcgc
     4201 aagcggtaac tcgtcacatg gttgaacgca aggcaggtaa agttattaat atttgctcga
     4261 tgcaaagcga actgggacgt gacaccatca ccccttatgc cgcatcgaaa ggggcggtaa
     4321 aaatgctcac ccgcggcatg tgcgtcgagc tggcgcgcca caatattcag gtcaacggta
     4381 ttgcgccggg ctatttcaaa acagaaatga ctaaagcact ggttgaggac gaagccttca
     4441 ccgcctggtt gtgcaaacgg acccccgccg cacgctgggg agatccgcag gaactgattg
     4501 gtgctgcggt gttcctttct tcaaaagcct ctgatttcgt aaacggccac ctgttgtttg
     4561 ttgatggcgg catgttagtg gctgtttaac agccaccacc ctacccacca ccctacgtgc
     4621 agcgcgaaag cgctgctttt ctggcactaa taagagataa agactatgcc attaatcatt
     4681 attgcggcag gcgtcgcgct gcttcttatc ctgatgatcg gctttaaagt taacggcttt
     4741 attgccctcg ttctggtagc tgccgtcgtc ggatttgccg aagggatgga tgcacaggcc
     4801 gtcctgcact ctatacaaaa tggtatcggc agcacgctcg gcgggctggc aatgatcctc
     4861 ggtttcgggg ccatgttagg caagctgatt tctgatacgg gcgcggcaca acgtatcgcc
     4921 actacgctga ttgctacttt tggtaaaaaa cgcgtgcaat gggcgctagt gatcaccggt
     4981 ctggttgtgg gcctcgccat gttttttgaa gtgggttttg tcctgctgtt gccgttggta
     5041 tttaccatcg tggcatcatc tggattaccc ctgttgtatg ttggcgtacc gatggtagca
     5101 gcgctctctg taacccactg ttttctgccg ccacatccag ggcctactgc catcgcgact
     5161 atctttgagg ctaatctcgg aacgacttta ctgtatggat ttatcattac cattccgaca
     5221 gttattgtcg caggaccgct gttttctaaa ctgctaactc gctttgagaa agcaccaccg
     5281 gaaggcttat ttaatcctca tctgtttagc gaagaggaga tgccctcctt ctggaacagt
     5341 atttttgctg cagtgatccc ggtcatcctg atggctatcg ccgccgtttg tgaaattacg
     5401 ttaccgaaaa ctaacaccgt gcgcctcttc tttgaatttg tcggtaaccc tgccgttgcg
     5461 ctgtttattg ccattgttat tgcgattttc acactgggcc gacgtaatgg acgcaccatc
     5521 gagcaaatca tggatatcat tggggattct ataggcgcta tcgcgatgat tgtgtttatt
     5581 atcgctggcg gcggcgcgtt taagcaggta ttagtagata gtggtgtcgg gcagtatatt
     5641 tcacacttaa tgaccggaac tacgctttcg ccgttattga tgtgctggac tgtcgcggcg
     5701 ctgttgcgta tcgctctggg ctctgccacc gtcgcggcca ttaccaccgc gggtgtggtg
     5761 ttgccgatta tcaacgttac ccatgccgat cccgctttaa tggtactggc aactggtgcg
     5821 ggcagcgtga tcgcgtcaca cgtaaacgac cctggcttct ggctatttaa agggtatttt
     5881 aatctgacgg ttggtgaaac gttgcgtacc tggacagtga tggaaaccct tatttctatt
     5941 atgggtttgc tgggcgtgtt agccattaac gccgtattgc actgaaagaa taaaatattg
     6001 tgatgtagtt tcgggggaag attgaagatt cccccgacgt tgggagaagt tatgcgcaat
     6061 cacagaattt ctttacagga tatcgctacg ctggctggcg taacaaaaat gaccgtgagt
     6121 cgttatatcc gctcgccgaa aaaggtggca aaggaaacag gcgagcgcat cgccaaaatt
     6181 atggaagaga tcaattatat ccctaatcgc gctcccggca tgttgttgaa tgcacaaagt
     6241 tataccctcg gtatcttgat tccgtcattt caaaatcaac tcttcgctga cattctcgcg
     6301 ggaatagaat ccgtcacctc tgtgcataat tatcagaccc ttattgctaa ttacaattac
     6361 gatcgcgatt ccgaagaaga gtcggtgatt aacctgctct cttataacat tgacgggatt
     6421 attctttcgg aaaaatatca caccatcagg acggtgaaat ttctgcgttc agccaccatt
     6481 ccggtcgtgg aattgatgga tgtacaggga gaacggctgg atatggaggt cggttttgat
     6541 aatcggcagg ctgcttttga catggtgtgt accatgctgg ataagcgagt caggcgcaaa
     6601 attctgtatc tcggttccaa agatgacacc cgcgatgaac agcgttatca ggggtattgc
     6661 gatgcgatga tgctgcataa tctttcccca ttacgcatga atccacgcgc catctcatcc
     6721 attcatttag gaatgcagtt aatgcgtgat gcccttagcg caaacccgga tttagacggt
     6781 gtattttgca ccaatgacga tattgcgatg ggtgcgcttc tgctgtgccg cgagcgaaac
     6841 ctcgccgtgc cagaacaaat ttccattgcg ggttttcatg ggctggagat tggcaggcaa
     6901 atgatcccaa gcctcgccag cgtaattact ccccgttttg atattggtcg gatggccgcc
     6961 caaatgttgc tgagcaagat taagaataac gatcacaacc acaacactgt tgacctcggg
     7021 tatcagattt atcacggtaa cacgctttag gttattttct cttcattaca catattctct
     7081 ttgccggacg ttgcctatac tccaggcagg aaactggagg aaatctcatg agcgaacccc
     7141 tgttaattgc ccgcacgccg gacacagaac tgtttttact gccgggaatg gctaaccgtc
     7201 acgggctgat tactggcgca acggggacgg gtaaaactgt cacgttgcaa aaactggcag
     7261 agtcattgtc ggaaatcggt gttccggtgt ttatggccga tgtgaaaggc gatctgaccg
     7321 gcatcgcgca ggcaggaacg gcgtcggaaa aactgctcgc aagactgaaa aatatcggcg
     7381 tcaacgactg gcaaccgcat gccaatccgg tggtggtgtg ggatatcttt ggcgagaaag
     7441 gccatccggt gcgggcgacg gtttcggatc tggggccgct gttgctggca cgactgttga
     7501 atctcaacga tgtgcaatct ggcgtgctga atattatctt ccgcattgcg gacgatcagg
     7561 gattgttgct gctcgacttt aaagatttgc gggcgattac ccagtacatc ggcgataacg
     7621 ccaaatcctt ccagaatcag tacggaaata tcagtagcgc atcggttggt gccatccagc
     7681 gcggattact gtcgctggaa cagcaaggcg cagaacactt ctttggtgag ccgatgctgg
     7741 atatcaaaga ctggatgcgc accgatgcca acggtaaagg cgttatcaat atcctcagcg
     7801 ccgagaaact ttatcagatg ccgaaactgt acgccgccag cctgctgtgg atgctttcag
     7861 agttatatga acaattgcct gaagcgggcg atctggagaa accaaaactg gtgtttttct
     7921 tcgacgaagc acatctgctg tttaacgatg cgccacaggt actgctggat aagattgagc
     7981 aggtgataag gcttattcgc tcaaaaggcg tgggcgtctg gttcgtttcg caaaacccgt
     8041 cagatatccc ggataacgtg ctagggcagc tcggtaatcg cgttcaacac gctttgcgtg
     8101 cttttacgcc taaagatcag aaagcagtga aggcagcggc gcaaaccatg cgggccaatc
     8161 cggcgtttga taccgaaaag gcaatccagg aactggggac cggcgaggcg ttaatctcgt
     8221 ttctcgatgc gaaaggaagt ccttcagtgg tggaacgggc gatggtgatc gcaccttgtt
     8281 cgcgaatggg gccggtgacg gaagatgagc gtaatggcct gattaatcac tccccggtgt
     8341 atggcaaata tgaagatgag gtggaccgcg agtccgccta tgagatgctg caaaaaggct
     8401 ttcaggccag taccgagcag caaaataatc cccccgtgaa agggaaagag gtggcggtgg
     8461 atgacggtat tcttggtgga ttgaaggata ttttgtttgg cactaccgga ccacgcggcg
     8521 ggaagaaaga tggtgtggtg caaacaatgg cgaaaagcgc cgctcgccag gtgacgaatc
     8581 agattgtacg cgggatgttg gggagtttgc tgggggggag aaaaaggtaa gtcagacgtg
     8641 atctgtgccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt cgaactcgta
     8701 tttgactttg tcaacaataa taaagggagc tttcgctccc ttgttagatt acgattttct
     8761 cattaacagc cacaggctga ttaagaagaa gctggcgctt ggcaacagtg cgccgatgat
     8821 cggcgggatg ccataaacca acgtcagcgg gccgaagatc tggtccagta cgtagaagac
     8881 aaaaccgaag ctgataccgg tgaccacacg cacgcccatc ggtacgctac gcagtgggcc
     8941 aaagatgaac gacagcgcca tcaacatcat caccgccacg gatagcggct ggaagatttt
     9001 gctccacatg ttgagctgat aacgtccggc atcctgaccg ctcgacttca gatacttcac
     9061 atagttgtgc aaaccactga tggagagtgc atccgggtcc agcgccacca cgcccagttt
     9121 gtctggcgtg aggttggttt tccaggtgcc gctcaccgtc tgcgaaccgg tgatctgttt
     9181 cggattggtc agatcagatt catcaacctg cgacagacgc cagactttat gttccgggtc
     9241 aaacttcgcg gtagcggcat aacgtacgga ttgcagacga cgattctcgt taaaggcata
     9301 aatactgatg ccacctaact cttcgtcacc tttaacccgc tcaatgtaga cgaagttgtt
     9361 gccatctttc gcccataagc cttgctgagt ggagagcaac gagccgccgt acatcgcctg
     9421 cgcacggtag ttacgcgcca tctgctcgcc ctgcgacgcg acccattcac caatcgccat
     9481 cgtcagcaag accagcggaa tggcggtttt catcaccgac agcgccacct gcatacgggt
     9541 aaaaccagaa gcctgcatca ccaccagttc gctgcgctgc gccagcatcc caagaccaag
     9601 caacgcccca agcagagccg ccatcgggaa gaagatttgc acatctttcg gcacgctcag
     9661 caaggtatac attcctgcgc ctaacgcgtc gtaactcccc tgcccggctt ttttcagctg
     9721 atcgacaaac ttgataatgc ccgacagcga caccagcatg aacagcgtca tcatgatggt
     9781 ggtgaaaata gttttaccga tatagcggtc aagtacgcca aaaggttgca tcacaccgct
     9841 cctttacgcg aaaaactggc gcgcaggcgg cggaccggca cggtgtccca aaggttgaga
     9901 acaatcgcca aagccagata aatcaggtta acggtccaca tccacagcgt cgggtccagc
     9961 ttacctttac cgccgttcga tttcagggag gtctggatca ggaagaaaag tagatacagc
    10021 agcatggctg gcagcatcga cagtacgcgt ccctgacgcg ggttaaccac gctcaacggt
    10081 acaaccataa gtgccatcat aaacacggtg aataccaacg tgatacgcca gttcagttct
    10141 gcgcgggcac gatcggtgtc agtgttccac aatgtgcgca tgtccatctg gtcggtatcg
    10201 ttcgggtcga gcaccaccgc ctggtgacca atgatcgcct gataatcctg aaagtccgta
    10261 atgcggaaat cgcgtaacag tgcagtgcct tcgaagcgcg ttccctggtt gagagtgacg
    10321 acctgggagc cgtcgcgcag ctgggttaaa tgtccggaat cggccaccac cacagaagga
    10381 cgtgcattac cttttggtcg aatttgtgcg aggaacacat ctttgaaatc gctgccgtca
    10441 acgctttcga tgaacagcac cgagctgcca ttagtcgctt gctggaattg cccttgcgcc
    10501 agcgccgcca tgccagggtt cgctttcgct tctgctaaca cttcatcctg atgacgcgat
    10561 gaccatggtc ccgcccacat cacgttaacc gccgcgacga ttgccgtgaa caccgcaagg
    10621 atcattgccg ctttcaccag caccgctttg ctcagaccgc aggcatgcat taccgtaatt
    10681 tcactttcgg tatacagttt gcccagcgtc atcagcagcc cgaggaacag gcttaatggc
    10741 aggataagct gcgccatttc cggcacgccc aacccgagaa gggagagcac cagattcgcc
    10801 ggaatatcgc cgtcaaccgc tgcgccgagg atcctcacta acttttgaca gaagaagatc
    10861 aaaagcaaga tgaagagtat cgccagctgg cttttgagcg tctcccgcac cagatatctt
    10921 atgattatca ctttaaatac gcccgtaaaa actcgtcttt tgcaggattt tagcttgttt
    10981 catggcttaa acgtcattta ttctcttgag tcgtcgaaat cgtcgctaag ataattatac
    11041 tcaacggatt cacctctcag attttgttct gacgtgccaa tgccgtaata acgttaagat
    11101 taacacgaag tcatcgcaac agcggacatg agttacgaaa gcttgcaatt ctatctgtag
    11161 ccaccgccgt tgtctttaag attcaggagc gtagtgcatg gagtttagtg taaaaagcgg
    11221 tagcccggag aaacagcgga gtgcctgcat cgtcgtgggc gtcttcgaac cacgtcgcct
    11281 ttctccgatt gcagaacagc tcgataaaat cagcgatggg tacatcagcg ccctgctacg
    11341 tcggggcgaa ctggaaggaa aaccggggca gacactgttg ctgcaccatg ttccgaatgt
    11401 actttccgag cgaattctcc ttattggttg cggcaaagaa cgtgagctgg atgaacgtca
    11461 gtacaagcag gttattcaga aaaccattaa tacgctgaat gatactggct caatggaagc
    11521 ggtctgcttt ctgactgaac tgcacgttaa aggccgtaac aactactgga aagtgcgtca
    11581 ggctgtcgag actgcaaaag agacgctcta cagtttcgat cagctgaaaa cgaacaagag
    11641 cgaaccgcgt cgtccgctgc gtaaaatggt gttcaacgtg ccgacccgcc gtgaactgac
    11701 cagcggtgag cgcgcgatcc agcacggtct ggcgattgcc gccgggatta aagcagcaaa
    11761 agatctcggc aatatgccgc cgaatatctg taacgccgct tacctcgctt cacaagcgcg
    11821 ccagctggct gacagctaca gcaagaatgt tatcacccgc gttatcggcg aacagcagat
    11881 gaaagagctg gggatgcact cttatctggc ggtcggtcag ggttcgcaaa acgaatcgct
    11941 gatgtcggtg attgagtaca aaggcaacgc gtcggaagat gcacgcccaa tcgtgctggt
    12001 gggtaaaggt ttaaccttcg actccggcgg tatctccatc aagccttcag aaggcatgga
    12061 tgagatgaag tacgatatgt gcggtgcggc agcggtttac ggcgtgatgc ggatggtcgc
    12121 agagctacaa ctgcctatta acgttattgg cgtgctggca ggctgtgaaa acatgcctgg
    12181 cgggcgtgcc tatcgtccgg gcgatgtgtt aaccaccatg tccggtcaaa ccgttgaagt
    12241 gctgaacacc gatgccgaag gccgcctggt actgtgcgac gtgttaactt acgttgaacg
    12301 ttttgagccg gaagcggtga ttgatgtggc gacgctgacc ggtgcctgcg tgatcgcgct
    12361 gggtcatcat attactggtc tgatggcgaa ccataatccg ctggcccatg aactgattgc
    12421 cgcgtctgaa caatccggtg accgcgcatg gcgcttaccg ctgggtgacg agtatcagga
    12481 acaactggag tccaattttg ccgatatggc gaacattggc ggtcgtcctg gtggggcgat
    12541 taccgcaggt tgcttcctgt cacgctttac ccgtaagtac aactgggcgc acctggatat
    12601 tgcaggaacc gcctggcgtt ctggtaaagc aaaaggcgca accggtcgtc cggtagcgtt
    12661 gctggcacag ttcctgttaa accgcgctgg gtttaacggc gaagagtaag tttgcgtttt
    12721 accctcaccc taaccctctc cctaagggag agggaaccga tcgagcgcaa tagaaattca
    12781 ttgctcatct ttcagggtga ggaatgcagc aatatttaaa tccaccacaa gaagccccat
    12841 atatgaaaaa cgcaacgttc taccttctgg acaatgacac caccgtcgat ggcttaagcg
    12901 ccgttgagca actggtgtgt gaaattgccg cagaacgttg gcgcagcggt aagcgcgtgc
    12961 tcatcgcctg tgaagatgaa aagcaggctt accggctgga tgaagccctg tgggcgcgtc
    13021 cggcagaaag ctttgtcccg cataatttag cgggagaagg accgcgcggc ggtgcgccgg
    13081 tggagatcgc ctggccgcaa aaacgcagca gcagcccgcg cgatatattg attagtctgc
    13141 gaacaagctt tgcagatttt gccactgctt tcacagaagt ggtagacttc gttccttatg
    13201 aagattctct gaaacaactg gcgcgcgaac gctataaagc ctaccgcgtg gctggtttca
    13261 acctgaatac ggcaacctgg aaataatgga aaagacatat aacccacaag atatcgaaca
    13321 gccgctttac gagcactggg aaaagcaggg ctactttaag cctaatggcg atgaaagcca
    13381 ggaaagtttc tgcatcatga tcccgccgcc gaacgtcacc ggcagtttgc atatgggtca
    13441 cgccttccag caaaccatca tggataccat gatccgctat cagcgcatgc agggcaaaaa
    13501 caccctatgg caggtcggta ctgaccacgc cgggatcgct acccagatgg tcgttgagcg
    13561 caagattgcc gcagaagaag gtaaaacccg tcacgactac ggccgcgaag ctttcatcga
    13621 caaaatctgg gaatggaaag cggagtccgg cggcaccatt acccgtcaga tgcgccgtct
    13681 cggcaactct gtcgactggg agcgtgaacg cttcacgatg gacgaaggcc tgtccaatgc
    13741 ggtgaaagaa gttttcgttc gtctgtataa agaagacctg atttaccgtg gcaaacgcct
    13801 ggtaaactgg gatccgaaac tgcgcaccgc tatctctgac ctggaagtgg aaaaccgcga
    13861 atcgaaaggt tcgatgtggc acatccgcta tccgctggct gacggcgcga aaaccgcaga
    13921 cggtaaagat tatctggtgg ttgcgactac ccgtccggaa accctgttgg gcgataccgg
    13981 cgtggccgtt aacccggaag atccgcgtta caaagacctg attggcaaat atgtcattct
    14041 accgctggtt aaccgtcgta ttccgatcgt tggcgacgaa cacgccgaca tggaaaaagg
    14101 caccggctgc gtgaaaatca ctccggcgca cgactttaac gactatgaag tgggtaaacg
    14161 tcacgcgctg ccgatgatca acatcctgac ctttgacggc gatatccgtg aaagcgccca
    14221 ggtgttcgat accaaaggta acgaatctga cgtttattcc agcgaaatcc cggcagagtt
    14281 ccagaaactc gagcgttttg ctgcacgtaa agcagtcgtt gctgcggttg acgcactcgg
    14341 cctgctggaa gaaattaaac cgcacgacct gaccgttcct tacggcgacc gtggcggcgt
    14401 agttatcgaa ccaatgctga ccgaccagtg gtacgtgcgt gccgatgtac tggcgaaacc
    14461 ggcggttgaa gcggttgaga acggcgacat tcagttcgta ccgaagcagt acgaaaacat
    14521 gtacttctcc tggatgcgcg atattcagga ctggtgtatc tctcgtcagc tgtggtgggg
    14581 tcaccgtatc cctgcatggt acgacgaagc aggtaacgtt tatgttggcc gcaacgaaga
    14641 agaagtgcgc aaggaaaata acctcggtgc cgacgttgcc ctgcgtcagg acgaagacgt
    14701 tctcgacacc tggttctctt ctgcgctgtg gaccttctct accctaggct ggccggaaaa
    14761 cactgacgca ctgcgccagt tccacccaac cagcgtgatg gtatccggct tcgacatcat
    14821 tttcttctgg attgcccgca tgatcatgat gaccatgcac ttcatcaaag atgaaaatgg
    14881 caaaccgcag gtgccgttcc acaccgttta catgaccggc ctgattcgtg acgacgaagg
    14941 ccagaagatg tccaaatcca agggtaacgt tattgacccg ctggatatgg ttgacggtat
    15001 ttcgctgccg gaactgctgg agaaacgtac cggcaatatg atgcagccgc agctggcgga
    15061 caaaatccgt aagcgcaccg agaagcagtt cccgaacggt attgagccgc acggcaccga
    15121 tgccctgcgc ttcaccctgg cggcgctggc gtctaccggt cgtgacatca actgggatat
    15181 gaagcgcctg gaaggttacc gtaacttctg taacaagctg tggaacgcca gccgctttgt
    15241 gctgatgaac acggaaggtc aggattgcgg cttcaacggt ggtgaaatga cgctgtcgct
    15301 ggcggaccgc tggattctgg cagagttcaa ccagaccatc aaagcgtacc gcgaagcgct
    15361 ggacagcttc cgcttcgata tcgctgcagg cattctgtat gagttcacct ggaaccagtt
    15421 ctgtgactgg tatctggagc tgactaagcc ggtaatgaac ggtggcaccg aagccgaact
    15481 gcgcggtact cgccatacgc tggtgaccgt actggaaggt ctgctgcgcc tcgcgcatcc
    15541 gatcattccg ttcatcaccg aaaccatctg gcagcgtgtg aaagtacttt gcggtatcac
    15601 tgccgacacc atcatgctgc agccgttccc gcagtacgat gcatctcagg ttgatgaagc
    15661 cgcactggcc gacaccgaat ggctgaaaca ggcgatcgtt gcggtacgta acatccgtgc
    15721 agaaatgaat atcgcgccgg gcaaaccgct ggagctgctg ctgcgtggtt gcagcgcgga
    15781 tgcagaacgt cgcgtaaatg aaaaccgtgg cttcctgcaa accctggcgc gtctggaaag
    15841 catcaccgtg ctgcctgccg atgacaaagg tccggtttcc gttacgaaga tcatcgacgg
    15901 cgcagagctg ctgatcccga tggctggcct catcaacaaa gaagatgagc tggcgcgtct
    15961 ggcgaaagaa gtggcgaaga tcgaaggcga aatcagccgt atcgagaaca agctggcgaa
    16021 tgaaggcttt gtcgcccgcg caccggaagc ggtcatcgcg aaagagcgtg agaagctgga
    16081 aggctatgcg gaagcgaaag cgaaactgat tgaacagcag gctgttatcg ccgcgctgta
    16141 atcaaaacac agtgatgaaa acgaaggccg gagcatgctc cggccttttt tatctcttac
    16201 agaaatggta aagaaggcat aattccccgt gaaatactgg ctaagaatcc tttatctggt
    16261 tgttcttctt tattgattaa aggaaggtca tccaaatcgc ccagcaaata cgtatttttt
    16321 gcctgccagt caatcatcca tattttcagc agtggataag ccagaccgcc cgtaatcccg
    16381 gatatcacca ccaacgcaca catgcgataa agcataccgt ggtatgttaa agttgagcga
    16441 aaacgaatac gcccatcatt cagtgacagg ttgctcataa aatgatttcg caacgacacc
    16501 gttaaataac tggtgctaac agcaatccca aaataataga ttaactgcgc gattatcatt
    16561 ttacgttgca tttccataaa ttgctgtaaa ttctcaatat catcatttgc atacccagaa
    16621 ctatcatact catttaatat ttgatcgaag ataatataac cagcaacagc gagaaaaggt
    16681 aataaagtca aaaatgcgag aatggcatat tttatacaat atgtagtatc taatttcacc
    16741 ttgaaacgat gtataccgaa actggtatta ctccagagaa aactcattac cagactatat
    16801 aaagtgccat taaaaatacc aatgaaaaca atacaaacta ttgtcatcag aaccacagat
    16861 ataataacgc tacttgaact attggcatgt agcatctttg tcgagataaa gaaaacagtc
    16921 cccatcccaa gggccattaa aatcggcaag aaaaaggtca cccaccagaa ccctttcata
    16981 gagcaattaa aactaaatct tacgccgttg agactggtca tcaaggcctg ataacgcagt
    17041 ccttttgctg ccataaatat aagcaaaacc aacagtgaca aagtcaaaac acagccaata
    17101 agcggcatat ctgctgacac tgtcattaag attgcgaaat agaaaaaaac aaaaacaaga
    17161 caactaacaa aaacattccc accggtaatc ccataagaaa atcgttgtcc gttaacttcc
    17221 atattagcat agagataacg cttacatttc attaatgccc atggtaaata gatacctagc
    17281 gttataatcg ttaacaacac attcaccaca caaataagaa aatatctctc acctgtacca
    17341 tgaaaaacaa acgaatggga cggaacatcc atatcattaa taacttgagc cataaaagtt
    17401 ccctgaatta aaaaatccat tgatataatt tatatataca gacagtaaaa tgttctgcc
//
LOCUS       DAZPEP010000079        17416 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZPEP010000079 DAZPEP010000000
VERSION     DAZPEP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17416
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.079"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(64..795)
                     /gene="rlmB"
                     /locus_tag="ACHUT9_002568"
     CDS             complement(64..795)
                     /gene="rlmB"
                     /locus_tag="ACHUT9_002568"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFR1349011.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(975..3416)
                     /gene="rnr"
                     /locus_tag="ACHUT9_002569"
     CDS             complement(975..3416)
                     /gene="rnr"
                     /locus_tag="ACHUT9_002569"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFR1349012.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3455..3880)
                     /gene="nsrR"
                     /locus_tag="ACHUT9_002570"
     CDS             complement(3455..3880)
                     /gene="nsrR"
                     /locus_tag="ACHUT9_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFR1349013.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4085..5383)
                     /gene="purA"
                     /locus_tag="ACHUT9_002571"
     CDS             complement(4085..5383)
                     /gene="purA"
                     /locus_tag="ACHUT9_002571"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFR1349014.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5487..5684)
                     /locus_tag="ACHUT9_002572"
     CDS             complement(5487..5684)
                     /locus_tag="ACHUT9_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFR1349015.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5766..6770)
                     /gene="hflC"
                     /locus_tag="ACHUT9_002573"
     CDS             complement(5766..6770)
                     /gene="hflC"
                     /locus_tag="ACHUT9_002573"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFR1349016.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6773..8032)
                     /gene="hflK"
                     /locus_tag="ACHUT9_002574"
     CDS             complement(6773..8032)
                     /gene="hflK"
                     /locus_tag="ACHUT9_002574"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFR1349017.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8118..9398)
                     /gene="hflX"
                     /locus_tag="ACHUT9_002575"
     CDS             complement(8118..9398)
                     /gene="hflX"
                     /locus_tag="ACHUT9_002575"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFR1349018.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9475..9783)
                     /gene="hfq"
                     /locus_tag="ACHUT9_002576"
     CDS             complement(9475..9783)
                     /gene="hfq"
                     /locus_tag="ACHUT9_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFR1349019.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9869..10819)
                     /gene="miaA"
                     /locus_tag="ACHUT9_002577"
     CDS             complement(9869..10819)
                     /gene="miaA"
                     /locus_tag="ACHUT9_002577"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFR1349020.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10812..12659)
                     /gene="mutL"
                     /locus_tag="ACHUT9_002578"
     CDS             complement(10812..12659)
                     /gene="mutL"
                     /locus_tag="ACHUT9_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFR1349021.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12669..14006)
                     /gene="amiB"
                     /locus_tag="ACHUT9_002579"
     CDS             complement(12669..14006)
                     /gene="amiB"
                     /locus_tag="ACHUT9_002579"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFR1349022.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14025..14486)
                     /gene="tsaE"
                     /locus_tag="ACHUT9_002580"
     CDS             complement(14025..14486)
                     /gene="tsaE"
                     /locus_tag="ACHUT9_002580"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFR1349023.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14458..16005)
                     /gene="nnr"
                     /locus_tag="ACHUT9_002581"
     CDS             complement(14458..16005)
                     /gene="nnr"
                     /locus_tag="ACHUT9_002581"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFR1349024.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16004..17143
                     /gene="queG"
                     /locus_tag="ACHUT9_002582"
     CDS             16004..17143
                     /gene="queG"
                     /locus_tag="ACHUT9_002582"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFR1349025.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17126..17179)
                     /locus_tag="ACHUT9_002583"
     CDS             complement(17126..17179)
                     /locus_tag="ACHUT9_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349026.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3947 a         4973 c         4372 g         4124 t
ORIGIN      
        1 atcttaccag ctatagacaa aaaaaccatc caaatctgga tggcttttca taattcagac
       61 gaactagctg cgctgacgaa ccgcttcaaa taagcaaatt ccggttgcaa ccgaaacgtt
      121 cagggaagaa acacttcctg ccattgggat gctgatcaac tcatcgcaat gttcacgggt
      181 caggcgacgc ataccttcac cttccgcgcc catcaccagc gccaagcgtc cggtcatttt
      241 gctctgatag agtgtatgat ccgcctcgcc tgccgtaccg acgatccaga tattctcttc
      301 ctgcaacata cgcatggtgc gcgcaaggtt agtcacccga atcagtggaa cgctttctgc
      361 cgcgccgcag gctacttttt tcgccgtagc gttgagctgt gcggagcgat ctttcggcac
      421 aatcaccgcg tgaacgccag cagcgtccgc gctacgcagg cacgcgccga ggttgtgcgg
      481 atcggttaca ccgtcgagga tcagcaggaa cggttgatcg agcgaagcga tcagatccgg
      541 cagatcgttt tcctgatact gacgtcctgg cttcacgcgg gcgataatgc cctgatgcac
      601 ggcaccgtcg cttttctcgt cgagatattg gcggtttgcc aactggataa ccacgccctg
      661 agactcaagg gcgtgaatca gcggtaacag acgtttatct tcacggcctt ttaaaataaa
      721 gacttcctga aaacgttcag gggcgcgctc cagcagggcc tgcactgcgt ggatgccgta
      781 aatcatttcg ctcattaatg tactcgttgt ttacgttttg cctgatgcac tacgtttatc
      841 cggcctacat gatctctgca atatattgaa tttgcgtgat tatgtaggcc ggataaggcg
      901 ttcacgccgc atccggcatg aacaaagcgc attttgtcag caatctaacc ctcttctttt
      961 aaagagggta ttgatcactc tgccactttt ttcttcgccg cacgcttcgc tttggtcgct
     1021 gcggctattt tctgcgtttt cgccgatggc tttttcgctt ttctcgcgtc tttcttcgcc
     1081 gctttcggct tcgttttttt ctcaccgcgg aaggcgctgt ctggctcaaa gtttaccttt
     1141 ttaccgacct gacgacgctt gccgcctttt ttgcctgcat cgcctttttt cgctttctcg
     1201 cgcgccgttt taccgacgtt gcgcggtgcg cgttcactgg agatcagact aaagtcgatt
     1261 ttgcgctcgt ccatattaac cgcttcgacg cgaacttcca cgcgatcgcc caggcgataa
     1321 gtctggccgc tggattcccc catcaggcgt tgccctacct ggtcaaagcg atagtagtca
     1381 ttgtccagcg aagagacatg gaccagacca tcaatgaaca agtcgtccag acggacgaag
     1441 aagccaaagc cagtgacgct ggaaattacg cctttaaaga cgttacctac ctggtcgagc
     1501 atgaagtcac acttcagcca gtcagcgaca tcgcgcgttg cttcgtcggc acgacgttcc
     1561 gccatcgaac agtgctgacc cagttgcaac atctcttcca tcgaataatg gtagccgccg
     1621 gtttcagtgg tgttgccctg atgcccctgc tctttcgcca gcagatactt aatggcgcgg
     1681 tgcagcgtca ggtcaggata acgacgaatc ggcgaagtaa agtgcgcata ggactgcaat
     1741 gccaggccaa agtgaccacg gttttctgga tcgtaaatcg cctgtttcat cgagcgcagc
     1801 agcatggttt gcagcatttc tgcatcagga cggtcggcaa ccgactccag cagttccgcg
     1861 tagtcacgcg gttccggctt gttaccaccc ggcagctcca gccccagctc cgccagcact
     1921 gaacggaaag aggtaatcgc ttcggtgctc ggcttgtcgt gaatacggaa cagtgccggt
     1981 tctttcgctt tctcaacgaa acgcgccgcc gagatattcg ccagaatcat gcactcttca
     2041 attaatttgt gcgcgtcgtt acgctgggtc tgttcgatac gttcaatacg gcgttcagcg
     2101 ttgaaaatga acttcgcttc ttcgctctca aatgagatcc caccgcgttc ttcacgggct
     2161 ttatccagca ctttatagag gttatgcaac tcttcgagat gcttaaccag cggggcgtac
     2221 tgctcgcgca gatcctgatc gccctgcaga atatgccaga ctttggtgta ggtcagacgc
     2281 gcgtgagagc tcatcaccgc ttcgtagaat ttgtagcccg tcaggcggcc tttcgacgaa
     2341 accgtcatct cgcacaccat acacaggcgg tctacctgcg ggttgagcga acacaggccg
     2401 ttagagagca cttccggcag catcgggata acctgcgaag ggaagtacac cgacgtgcca
     2461 cggttacgcg cttctctgtc cagcggcgtt ggcggacgca catagtagct gacgtcggca
     2521 atcgcgaccc ataaacgcca gccgccgccg cgttttttct cgcagtaaac tgcatcgtca
     2581 aagtcacggg cgtcttcgcc atcaatggtg accagcggta aatcgcgcaa atcgacacgg
     2641 cccgcttttg cttcttccgg cacttcttct ttcagccccg caacctgttg ctcaacagcc
     2701 tgcggccaga tatacggaat ttcatgggta cgcagagcga tatcaaccgc catgccggtg
     2761 cccatattgt cgcccagcac ttcgacgatt ttacccaccg ctttggtgcg gcgagtcgga
     2821 cgctgagtca gttcgactac gaccacaaag cccatccgcg cgcccatgat ctgatcgggc
     2881 gggattaaga tatcgaagct cagacggcta tcgtcaggaa ccacaaagcc gacgcccgct
     2941 tcggtaaagt agcgaccaac aatctggctg gtttttggca ccagtacgcg gacaatacgc
     3001 gcttcacgac gacctttacg gtcagcaccc agcggctgcg ccagcacctg atcgccatga
     3061 atacaggttt tcatctgctc gctggagaga tacaaatcat ctttacgccc ttcaacccgc
     3121 agaaagccgt agccatcacg gtggccaata acggtacctt tcaccaggtc gaggcgttcc
     3181 ggcagcgcat agcactgacg acgagtgaag accagttgac catcgcgctc catcgcgcgc
     3241 aggcggcgac gcaggccttc aagctgctct tcgccttcaa tgtgcagttc taccgccagc
     3301 tcatcacggc tggccggttt ttcacgtttg gttaaatgtt cgaggataaa ttcccgacta
     3361 gggatgggat tcgcgtattt ttcagcttcg cgttcctgga aaggatcttg tgacatctcg
     3421 gttcctccgt tgtcatctct gatgaagatt ttcgtcactc caccagcaat aatttataaa
     3481 gcggttgatt ctcttcaacc aaatcggcaa gcgtgtagtt atccagttcc gtaagaaaac
     3541 tttgcacggc cttagaaagc gcctgtttca accgacaggc aggtgtaatg tggcaaaact
     3601 cactgctgca attcaccagc gataagggct ccagctcgcg caccacatca ccaatacgta
     3661 tcgcactcgc cggtttaccc aggcgaatgc cgccattttt tccacgaaca gcagtcacgt
     3721 agccggcacg actaagttga ttgattattt tgaccatatg attacgggag acgccgtaga
     3781 cgtcagtcac ttcagaaata ctggtcatcc gcccttctgg caatgacgcc atgtagatca
     3841 gcgcacgtaa tccgtaatca gtgaaactcg ttaactgcac atcaacctca aaagggaaat
     3901 cgggaaaaaa tacatttata ttgatgataa accagccaca agctgtgtcg ctaatttatt
     3961 tcagttcggg gaaggaaaaa agcgaggatt taacactgtg tcggatagcg taatggctta
     4021 accgacacag caatgtatca cactgttagc ccggcaggca aaatatctgc caggcgtacc
     4081 agaattacgc gtcgaacgga tcgcgcagaa tcatggtttc agtacgatcc ggaccggtag
     4141 agatgatatc gatcggcaca ccggtcagct cttcaatacg cttgatgtag ttcagcgccg
     4201 cctgcggcag gccgctacga tctttcacgc cgaaagtgga ttcagaccag cccggcatgg
     4261 tttcgtaaat cggctctaca cctttccagt cgtcagctgc cagcggagtg gtagccactt
     4321 cgcgaccatc cggcatacgg taagccacgc agagtttaac ctctttcagg ccatccagaa
     4381 cgtccagctt ggtcaggcag aagccagaca gggagttcag ctgtaccgca cgacgaacgg
     4441 caacggtgtc cagccagccg gtacgacgac gacgacccgt agttgcgccg aattcgttac
     4501 cctgcttgca gaggaactcg ccagtttcat caaacagttc ggtcgggaac ggacctgcac
     4561 ctacacgagt tgagtaagct ttgaggatac ccagaacata atcaacataa cgcgggccca
     4621 ggccggaacc ggtcgccacg ccaccagcag tggtgttgga agaagttacg tacggataag
     4681 taccgtggtc gatatccagc agcgtaccct gcgcaccttc aaacatgacg aaatcgccac
     4741 gctgacgcgc ctggtcgagc aggtcagaaa cgtcaaccac catagaagtc aggatgtcgg
     4801 caacagccat cgtatcatcc agaactttct ggtaatcaac cgcttcagct ttgtagtagt
     4861 taaccaactg gaagttgtga tattccatca cttctttcag tttttcagcg aaggtttctt
     4921 tgtcgaaaag gtcgccaaca cgcagaccgc gacgtgctac tttatcttca taagcaggcc
     4981 cgataccacg accggtggtg ccgatcgctt tcgcgccacg cgctttctca cgcgcgttat
     5041 ccagcgcaac gtgataatca aggatcagcg gacatgcttc agacagcagc agacgctcac
     5101 gaacggggat gccacggtct tccagttctt tcatctcttt catcagcgcg gccggagaca
     5161 gcacaacacc gttaccgatg atgctggtta cattctcgcg gagaatacct gatggaataa
     5221 gatggagaac ggttttttca ccgttgatta cgagagtatg gcctgcgttg tgaccgccct
     5281 ggtagcgtac aacatattta gcccgttcag tcagaagatc gacgatctta cctttacctt
     5341 cgtcacccca ttgggtgccc agtacgacga cgttgttacc catttttcaa aatcaccgtt
     5401 tgcttaaaaa tggattctac catcgctttt tcagagttac agcacttttt gcactcaaaa
     5461 atgacctcga tcagtctgtt ttttgttcag ccaatcgttt tcctcaacat gtagtagacc
     5521 acaacgcccg caaccacaag tccaccgcca aaacgacgta aaatattatc gggcaaattg
     5581 gtcatcgcag agatcatctt cttccatgcc ttcgggtaaa gcatcggccc taaaccttcc
     5641 agtaccaaaa ccagggcaag cgccagccag attgtcgaat tcattcatta tccttataga
     5701 aaaagaaaac caccgacatc cctgaggatg cggtggcttt attgacctgt accgcagtcg
     5761 ttatattaac gcgttgcgga agtcggcgtc ttcatgtagc ggaagaaatc gctatccggg
     5821 ctcatgacca tcacgtcctg attgccagag aagctgttct cataagcacg caggctacgg
     5881 atgaatgcgt agaagtccgg atctttactg aatgcatcag caaacagttt ggctgcttcg
     5941 gcatcgcctt caccacgcat gatgcggccc tgacgctcag cttctgccag cgttctggtc
     6001 acttcatagt cggcagtcgc gcgcagtttt tccgcttctt cctgaccttg tgaacggtga
     6061 cgacgcgcta ccgcttcacg ctcggcgcgc atacggttgt agatcgcttc agacacttcg
     6121 gtcggcaggt tgatctgctt gatacgcaca tcgacaactt caatacccag cgccgccata
     6181 ctgttcgggt tgatgaccgg aactttgccc ttcgtctcag ccgttacgcg ctctgccgct
     6241 tcggcaatgg cgttatctgc cgccggggta gtaacttcat cttctgtacc cgcagaaccg
     6301 gagttcagcg cgtcacgtac ttcgagggtc agacgaccac gggaatcggt gacgatgtct
     6361 ttcacgtcca ggcgaccaat ttcagaacgc agacggtcag agaacttacg tttcaacagc
     6421 acttccgctt gcgaaatgtc gccaccaccc gttgccaggt agtaacggct gaaatcgctg
     6481 atgcgccatt tgatgtaaga atcgacgatc aggtctttct tctctttggt cacaaagcgg
     6541 tcggcctggt tgtccatggt ctgaatacgt gcgtcgagca ttttcaccgt ttcaatgaac
     6601 ggtatcttga aatgcagacc cggctcataa accagaggtt tgttgtcatc gtcacgcagt
     6661 accttaccaa aacgcagcgt aataccgcgc tcaccttctt tgacgacaaa gacagacatg
     6721 taaagcacta ccagcacgat gatgataatc gcgataactg acttacgcat cgttattccc
     6781 cctgacgctg gtagtcgtta cgctgcgcgt tggcgcggcg ttggtccata atatcgccct
     6841 gactggtgga cgacgtgttg cttgctccac tggttgtgga ggaagaggct ggcggcagac
     6901 gcagcagatt gctggcaccg ttatcgctct tcgccgcagg ggcgttaccg cctttcagca
     6961 tctggtctaa cggcagaacc atcaggttgc cacctttatc gttaaccagc actttgcggg
     7021 tgttacccaa cactttttcc atcgtctcga tatacagacg ctcgcgagta atttccggcg
     7081 cggctttata ttccggcaga agtttagcaa agcgcgccac ttcaccctga gcttccagga
     7141 tggtctgggc cttgtacgca cgcgcctctt cgaggatacg ttgcgcctga ccgttcgcac
     7201 gcggctgaac ttcgttggta tacgcttctg cttcacgaat gtattgctgt tcgttttcac
     7261 gcgcggcaat cgcatcgtca aacgccgctt ttacttcttc cggcggacga gctgcctgga
     7321 agttgacgtc cagcagagtg atacccatgt catacggacg gatagtctct tccagttcgc
     7381 gctgagtatc gctacgaatc acggtacgac cttccgtcag aatgcggtcc atggtgtatt
     7441 taccgataac tccacgcagg gcgctgtcgg tagcctgacg caggctgtca tccgggctgg
     7501 tcacgctata cagatatttt tccggattgg tgacgcggta ctgcacgttc atctcaacgc
     7561 gcactacgtt ctcgtccgac gtcagcatca caccagaagc ggccagttca cgcacggctt
     7621 ccacgttcac cggtttgact tcgtcgataa acgtcggttt ccagttcaga cccggctcaa
     7681 ccagatggct gaatttacca aagcgtgtta ccacgccgcg ttcggcttct ttaatggtat
     7741 agaaaccact ggccgcccag ataatgacaa tcgctgccgc tgcgatggta acgacacgac
     7801 cgccaagctg cgggcgcggg ccttgcgatg aactgccacc gccagatccg gtgcctttac
     7861 cgccgcccag accaccgagc tttttgctca gtttgcggaa gatatcatct aaatcaggtg
     7921 gcccttgatc gcgaccgcct ttgtttccat ttccctcaga gttgccgcca ggtttgctgc
     7981 ttccccacgg gtcgcggtct tgtccgttat taccgggctg attccacgcc atgtttgtgc
     8041 tccatatttg ttatgcggtg atccctgttg tctttcgacg ccagaggata tgactccacg
     8101 cttcagacgc tacgccgtta gatcaggtaa tcgatcaacg ccggttcttg tttacagagg
     8161 cgacgccagt caacgatcgg catacgaact tgcagactta cgctgccgtc ctcctccatc
     8221 cactcttttt ctattgcctg aagctgataa aaacgacttc tcagacgtcc ttcctgcggc
     8281 ggcaaacgca atgtatgctg cgccacctcg ccggaaagcc gctccgtcaa agcctgaaaa
     8341 agctgtggta tccccgctcc ggtctgtgcg gaaagccaga cacggatcgg tttgttctct
     8401 tcgtcccgat caatacgcgg ttcgaaatct tccagcatat cgatcttgtt catcaccagc
     8461 agggttggga tctcgtgagc gtcgatctct tcaagaaccg tattcaccgc ttcgatgttt
     8521 tcttgtacac gtacatccgc cgcatcaatg acgtgcagca gtaatgtggc ttgccgcgtc
     8581 tcttgtaacg tggctttaaa tgctgccacc agatcgtgcg gcaggtggcg aataaaccct
     8641 acggtatctg caagtacggt ttcaccgaca tccgcgacgt caatacgccg caacgtcggg
     8701 tcgagggtgg caaacaactg gtctgccgcg tagacccgcg cttcggtgat gcgattgaaa
     8761 agggtagatt taccggcgtt ggtatatccc accagcgaaa cagtaggaac gtcggctttg
     8821 atacgcgatt gccgcccctg ctcacgctgc ttttcaactc tttctaggcg cgactgtatc
     8881 tgcacgatgc gattacgcaa caaacgacgg tcggtttcga gctgggtttc acccggacca
     8941 cgcaaaccta tcccgccttt ctgtctttca aggtgggtcc agccacgcac caggcgcgta
     9001 gccagatggc gcagctgcgc cagctcaacc tgcaacttac cctcatgggt acgcgcacgt
     9061 tgggcgaaaa tatctaaaat aaggccggtg cggtcgataa cacgacactc gcacaaacgc
     9121 tccaggttac gctcttgcgc cgggctcagg gcatggtcaa aaagaacgac cgaagcaccc
     9181 gtcgctttga cagcttccgc aatttcaact gctttacctt cacctacaaa atactttggg
     9241 tgcggcgctt tacggctacc ggtaatcacc tgcaatgctt cgacaccggc ggaagagacc
     9301 agagattcaa actcctggag gtcttccata tctttgtctt gcgtaaaata gatgtgtacc
     9361 agtaccgcct gctcaccagc atcataacgg tcaaacaagc gtataaccct ctaaatagat
     9421 cagcggggaa cgcaggatcg ctggctcccc gtgtaaaaaa aacagcccga aaccttattc
     9481 ggtttcttcg ctgtcctgtt gcgcggaagt attctgcgcg ctgctaccat gatggtagtt
     9541 actgctggta ccgccaccgg cgttgttact gtgatgagaa accgggcgag acgggacaac
     9601 agtagaaatc gcgtgcttgt aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa
     9661 ctgatcaaaa gactcgattt gcccttgcag cttaatacca ttcaccaaat aaatagaaac
     9721 tggaacacgt tcccgacgca gtgcgttcag gaacggatct tgtaaagatt gccccttagc
     9781 cattctctct tttccttata tgcttatttg tactttgaac ctttcgattc tgaaaaaatt
     9841 gcgcacgata cgtctcaatt gtacacattc agcctgcgat agcaccaaca acctgtaata
     9901 cttcgtcacg cgcctgttct ggtttttcac tgtcaagcca gtgaacccct tcccaaccac
     9961 gcagccaggt tatctgccgc ttcgccaact gtctcgtggc gcaaacacct cgataaacca
    10021 tttcatcgta tgagatttcg ccttcaaggt aagaccacat ctggcgataa cccacgcaac
    10081 gaatggaagg caagtccgta tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt
    10141 caaaacctga agccaacatc tgatgaaaac gctgctcaat gcgttgatgg agcagttcac
    10201 ggctcgccgg ggcgatggcg aactgatgca cctgatacgg tagagcgtct cctgacgttt
    10261 gcgtcagttc cgttaaagtt ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc
    10321 tttgtggatc atttggatga atccttgctg ccgcaaccgg atctacctcc tgaagttgac
    10381 gatgcaatga ctcccaacct tgctctgccg cctgttgctc aattctggcc cgcacttccg
    10441 ggtctgccga cggtagcggc gacaaccctt ccagcaatgc cttgaaatac aacatcgtac
    10501 cgcccactaa cagtgggatc cgccccgccg cggtgatatc ggccatttcc gccagcgcat
    10561 cgcggcgaaa atcagcagcc gagtaagcct gcgacggatc gcgaatatcc agcaatcggt
    10621 gcggcgcggc gagtaactct tcagcgttcg gcttcgccgt cccgatatcc atccctttgt
    10681 aaataagggc agaatcaacg cttatcaact ctactggtaa aattttacgc agctcaatgg
    10741 ctaacgccgt tttaccggag gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg
    10801 ccttactgat atcactcatc tttcagggct tttatcgccg gatgtaaatc aacagattgt
    10861 aacagaccac ccggcggcgt tttcacaagt tgcggacata accgttccac gtccgccagc
    10921 agggttatgg cctgtgccat tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc
    10981 cactgcgcaa tattgccagg ttcgaatacg gactgcttcg ccaggtagcc tatcagttca
    11041 ggaatcaaga tttgtaaatt ttgttggcgt aagggtaaag gcacggccct gatggtcaca
    11101 tgctgtgcat ctgactggaa atcaataccc aattccgcca gggcagactg cgctttttct
    11161 aatgccgatt tttcttcgcc agaaactttt agccgcaacg gaataagcag cggctgagcg
    11221 caaactggcg cttcacccgg cgtcagttgt acctgacgca gccaacgttc tgccactggc
    11281 aaggctaaaa gtgaaatgtt gccgtcgcgc tccagcaacg cacagtcgga atggacgata
    11341 gtcagtaccc gaccaaaact ctgactgttc gccgcaagtg caggttcctg cggttccggc
    11401 gcttttaatt tttgcatcgg cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc
    11461 tgtttctggt agcctggctg cgcattcggc cagggggcag ccggacgact gcctgatgct
    11521 ggcgcaggag tgtagcgcgg agctaccggc tcacgagctg ccggttctgc aaagtgattg
    11581 cgccccgccg ccacgcggtt ttccggaatg gaacgcggtg caggttgggg ttcatcgtcc
    11641 agcggtagcg gcgtttccag ttgctgttgt agcacgctca gcacgccctg atagataaaa
    11701 tcatgcacca gacgcgactg atggaaacgc acttcgtgtt tggcggggtg cacgttgacg
    11761 tccacctgat gcggatcgat ctccagatac agcacaaatg ccggttgctg atcggccccc
    11821 agtttgtctt cgcaggcctg gcggatcgcg tgattgatca ggcgatcgcg catcatgcga
    11881 ccgttcacgt agcaatactg aatttctgcc agtgcgggcg tggtgtgatt tggatcggcc
    11941 acccagccgc gtagcgtgag atcgccgtgt tgccattcaa tcgccagcgc ttgttcaaga
    12001 aaagcggtgc cgcaaatcgc gcctaagcgc cgttcttttt gcccgccttc cggcactgcg
    12061 cggtactgac gcacaatttt accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc
    12121 agcgcaatgc ggcggatgat ctcatcaatg tggttaaatt cggttttctc ggtgcgcagg
    12181 aatttgcgcc gcgccggggt gttgtagaac agatccagca cctccagcgt cgtccccaca
    12241 ggatgcgccg ccggttttac cgtcacgttc atatcgcgcc cttcggcata ggcctgccag
    12301 gcttcctgct gttctgcggt gcgtgaagtg agcgtcaggc gggaaaccga actgatactc
    12361 gccagcgcct caccgcgaaa gcccaggctg ataatggctt cgagatcgtc cagagaggcg
    12421 attttactgg tggcatgacg agccagcgcc agcgccagct catctttttt gataccgcag
    12481 ccgttatcac gaatgcggat aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgc
    12541 gtcgcacctg catcgaggct gttttccacc agttctttga ctaccgacgc aggtcgctcg
    12601 accacctcac ctgcggcaat ctggttcgcc agttgtggcg gtaagacctg aattggcatc
    12661 aatcgtcctt agtttggcag cgtgcgatct ggcgtcgtca ccgtactggc agtttgtgcc
    12721 gttgccccct gcggcgcaga ttgcatcgga tgcgcaagga aataattgcg caggcctttg
    12781 taaatggctt ctgccagctg ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg
    12841 ttgttgctga taaaaccggt ttcgaccagt actgacggga tatccggcga acgcagaacg
    12901 ccgagactcg cgtgttctgg tcggcgttta tgtatttcgc caatgcgttg caactgactg
    12961 atcatactgg tcgctacatc ataccctacc cgctgggaat gaccgaactg taaatccagc
    13021 accgcctggc ttaaataggg gtcagactga ctgttcgcca gcacatcgcc cgccccaccc
    13081 agcagctccg actgtttctc gtgctgctcc agccagctgg ccatttcgct gttggcgcga
    13141 cggttagaga gcacccatac ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg
    13201 tgaatcgaca cgaggaaatt ggcgttttgc ttacgtgcca catcgctgcg ccccatcacc
    13261 gagataaagt aatccccgtc acgggttaaa acgcctttaa acatcggatc gtcattgagc
    13321 aaagtgcgca atttacgcgc gatggcgatg gtgacatttt tctcccgcgt accaccgggg
    13381 ccgatagcgc cagggtcctg accgccgtgt ccggcatcaa tagcgatgat aattttatcg
    13441 ccagtgttag ccgtcgcgcg cgctgccgga cgcgttaccg tattactgct gataacaccc
    13501 gtagtgcggt tactttccgt tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg
    13561 acaaccgcag gcgtttcaac gcgtttcgca accacgggcg gtggaggagg cggtggcggc
    13621 acatcggcgt taatcgtaaa gacgacagtg taattgctgc cattctgccg cttcaccgct
    13681 tcggttttac cgttttcggt aagatcgacc accagccgta gcgtttgtgc atctttaggc
    13741 gttccagagc gaatcgcctt caccagatta ttgccgctga acaacaacgg cagtccctga
    13801 atcacgcccg tttgtttgat atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca
    13861 taatcaggat cgccaataaa actcaacgtt atccgcgcct gttgattacc gttagaaacc
    13921 tgaatatcag agagcgtcgc ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc
    13981 aaccaatttc tgatgcgata catcatcccg ccacctttca aaggttaacc ggctaaacgc
    14041 gccagcaaca attcacccgc agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc
    14101 tgataatcaa tgtgtatttc gacatccggg tcaggaagaa cacctgtacc ttgttgtggc
    14161 cactccacca ggcagatggc atcgttggca aaataatcgc ggatccccat aaactccagc
    14221 tcctcgggat cggcaaggcg gtacaaatca aagtgataaa ccattaaatt gtcgagcgta
    14281 tagggttcga ccagcgtata agtggggctt ttgacattac cctgatgacc cagagcttgt
    14341 aaaaaacccc ggctaaaggt ggttttacct gcgcctaaat cgccatacag atagattacg
    14401 gttgcgccat cgcaggcttt cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca
    14461 gggagcggaa ttactcgatt catcatggtt tttatcagtc acttccgggt taacaatacg
    14521 ctgtagcgtg gaaaagagat cggttgccag catcccgcgc gttccaaaac gcgccgccag
    14581 tacgtcagct gccgcaccgt gcgcgacaca gcccgcacag gcggcatcat acggcgacag
    14641 tttttgccca agcaatgcgc caataatacc agagagcaca tcgcccatgc cgccgctcgc
    14701 catgcctgca tttccggcat caataatgcc taaagcgtca ggatgggcgg cgaccacggt
    14761 tccggcacct ttcagcaccg ctacgccgcc ataacgttgt accagacgtt tggcgcaatg
    14821 taagcggtca ctttcaattt cagcgacgga acagcctaac aaccgtgcgg cctcgccagg
    14881 atgcggcgtg atcacgcgat tgtgacgctt atcgggattg attgccagca ggttcaatgc
    14941 atcggcatcc cacaacatcg gtttgcgaaa attctcaact ttttgcagtg cttttttccc
    15001 ccactcttgc tggcccagac cgggaccaat caccaccaca tcggcccatt ccaggctttc
    15061 ggtaagagag tccatcgtca gttcatgcac catcaattcc ggtcgtgcag tcagcagcgg
    15121 cgcaatgttt tcactgcggg tcagtactcg gactaaacca gcaccagcac gcagcgccgc
    15181 ttcccccgtc atacgaatag cccccgccgt gccgtgatcg ccaccgataa tcaccagccg
    15241 cccgtgatcg cctttatgcg aagtcgggcg acgcggtttc agccagtgag aaagttgttc
    15301 tgccgaaaac cgctgaattt tcgtctcctg acctgccagc cagctatcca gccccagtga
    15361 gtcaaaatgc agttgtcctg taacatcccg cgcttttcca gtgagcaagc ctggtttcag
    15421 cgcaataaaa gtgatggtgt gatcggcgtt gatcactgcg cctggcgtag cgccggtttc
    15481 agccagcagg ccggaaggga tatcaaccgc cacaatcggc gcaggatggg aattagcgtg
    15541 gtcgattaac tggctaatgg attcgcgggg cgcttgctgc aagccggtac cgagcagcgc
    15601 atcaacaatc agatctaccg attcgggcca gacaatattc gaagcatgga tctcgccacc
    15661 cgcgtttaac catgcttcgc gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc
    15721 ctgggccaac aacgtgacct caatgccgac cgctttggcc agtcgcgcga ccacgtagcc
    15781 atcgccgccg ttattaccat gaccgcacag caccagccag tggcgggcgt caggatacgc
    15841 cgaacgacac acctggaatg cggcctcgcc agcgcgaagc atcagctcat agagtgtgag
    15901 ccccagcaca tctgccgcct cgcgttctcc gcggcggata tcgtcggcgt accagacggt
    15961 gtgtggtata cttacggggt ttttcttcat tgtatggtcc gtcatgtcag agcccctcga
    16021 tctcaatcag ttagcgcaaa aaattaaaca gtgggggctg gaactgggct ttcagcaggt
    16081 aggtattacc gataccgatc tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa
    16141 acaataccac ggcgaaatgg actggatggc acgtcacggt atgctgcgcg ctcgccccca
    16201 tgagttatta ccaggtacgc tgcgtgtaat cagcgtgcgg atgaattacc ttcctgctaa
    16261 cgccgcattt gccagcacgc tgaaaaaccc caaactcggc tatgttagcc gttatgcgct
    16321 gggccgtgac tatcacaaac ttctgcgcaa ccgactcaaa aagctgggcg agatgattca
    16381 gcaacattgt gtttcgctga attttagacc gtttgtcgat tctgcgccta ttctcgagcg
    16441 cccgttagct gaaaaagctg ggctcggctg gacaggtaag cactcactta tcctcaatcg
    16501 cgaggccggt tctttcttct ttttaggcga attgctggtc gatattccgc tgcccgtgga
    16561 tcaaccagtc gaggaaggat gcggtaaatg catagcctgt atgacgattt gcccgaccgg
    16621 tgccatcgtc gagccatata ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga
    16681 acttgaaggg gcgatcccgg aagagttgcg accgttaatg ggaaaccgta tttacggttg
    16741 cgatgactgc cagcttatct gcccgtggaa tcgctattcg caactcacta cagaagacga
    16801 tttcagcccg cgtaagccgc tacacgcaca ggaactcatt gagttatttg cctggagcga
    16861 agagaagttt ttaaaagtca cggaaggatc ggcgattcgc cgtataggtc acctgcgctg
    16921 gctgcgtaat atcgccgtgg cattaggcaa tgcgccctgg gatgaaacga ttctggcggc
    16981 gctggagagt cgcaaaggtg agcacccact tcttgatgag cacatagcgt gggcgattgc
    17041 gcagcaaatc gagagacgaa atgcgtgcat agtcgaagtg caattgccga aaaaacagcg
    17101 tctggttcgg gtgattgaaa aagggttacc gcgtgacgcc tgattcatcc acagcttgtg
    17161 aataaaaata aaaacgcatt gcaattcaag acgcataaaa tcgtcaagtg atcgactcga
    17221 caaaacagaa tcttgatttt taacttattt atcaataggt taaaaaaata ccattcaccg
    17281 tgcgaagttt ctttgaaatt aagattacag cctgaggatg tggataactc tgtttacaag
    17341 agtttgtcag agacaacaaa aaccagcaat aacgcatcgc gtcgctgtgg atattttatt
    17401 gagagaagaa tttgga
//
LOCUS       DAZPEP010000080        17338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZPEP010000080 DAZPEP010000000
VERSION     DAZPEP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.080"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            92..1132
                     /locus_tag="ACHUT9_002584"
     CDS             92..1132
                     /locus_tag="ACHUT9_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFR1349027.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUT9_002585"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="ACHUT9_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFR1349028.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUT9_002586"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="ACHUT9_002586"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFR1349029.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="ACHUT9_002587"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="ACHUT9_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFR1349030.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUT9_002588"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="ACHUT9_002588"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFR1349031.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUT9_002589"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="ACHUT9_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFR1349032.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHUT9_002590"
     CDS             complement(8439..9131)
                     /gene="ygeA"
                     /locus_tag="ACHUT9_002590"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFR1349033.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHUT9_002591"
     CDS             complement(9262..10680)
                     /gene="araE"
                     /locus_tag="ACHUT9_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFR1349034.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHUT9_002592"
     CDS             complement(10995..11756)
                     /gene="kduD"
                     /locus_tag="ACHUT9_002592"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFR1349035.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="ACHUT9_002593"
     CDS             complement(11821..12657)
                     /gene="kduI"
                     /locus_tag="ACHUT9_002593"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFR1349036.1"
                     /translation="MDVRQGIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12944..14125)
                     /locus_tag="ACHUT9_002594"
     CDS             complement(12944..14125)
                     /locus_tag="ACHUT9_002594"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFR1349037.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14380..15609
                     /locus_tag="ACHUT9_002595"
     CDS             14380..15609
                     /locus_tag="ACHUT9_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1349038.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            16069..16701
                     /locus_tag="ACHUT9_002596"
     CDS             16069..16701
                     /locus_tag="ACHUT9_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1349039.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            17035..>17338
                     /locus_tag="ACHUT9_002597"
     CDS             17035..>17338
                     /locus_tag="ACHUT9_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1349040.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVTQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPREDS"
BASE COUNT         4595 a         4523 c         3989 g         4231 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctgg gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acacggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggcacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt cgccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt gttccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgca
     8821 atgtgtaaga aaggcagagt gcaacgtgac tcaatggcat ccgccacttt atgcatcgta
     8881 ttggtacata acacaatacc ttctgcgccc gcccgctgta agccaagcgc cgcctcagcc
     8941 agaatgtccc cggttttatc ccattccccg cgacgctggc actcttctat ttcatgaaaa
     9001 tcgacgctat gtagcagcac ttgcgcagag tgaagcccac caagccgctg tttaatgcct
     9061 tcatttatca gacgatagta aggaatggtg gattcccagc tcattcctcc cagcaaacca
     9121 attgttttca ttcctctctc tcctgatgtg tgttaccgca acaaggctga ataaggaacg
     9181 agacaaacgc ctcaacggcc aggtgcccaa tctctattaa cgaaaaaagg gccggatgta
     9241 cagcacatcc ggcccgtgaa atcagacgcc gatatttctc aacttctcgc ctgccatcag
     9301 tttgcgttcg atatgttcca gcgtgacatt tttggtttcc ggaatgagcc agaaagtgat
     9361 gcccacaaac gcaatgttca gcgcagtgta gagccagaac gtaccggcag caccaatgct
     9421 gtcgagcagc gtcaggaagg tcgcgccgat aatcatattc gacacccagt tcgtggtggt
     9481 cgaacaggta ataccgaaat cgcggcattt cagcggctga atttcagagc acaggatcca
     9541 caccactggc gcggcgctca tcgcataacc ggcaatacac atcatcgtca tgccaacaga
     9601 gagccaggac aagccactgg aagccgtacc gttatcaaac tgcatcaggc aatagcccag
     9661 caccagagtg cctaacgcca tcacgctgaa accaattttc agagccggtt tacgccctgc
     9721 tttatctacc gtaaacaccg caataaaggt ggcgaacata aaggtcagcc ctacgaccag
     9781 agtcgcaatc atctgttgtt ctgtggtcgt aaagcccgcc attttgaaga tacgcggcgc
     9841 gtagtacatg atgatgttca taccggtaaa ctgctgcatc gcctgcaaca acataccgag
     9901 aaacacagca cgacggacgt tacggttgat cttaaacagt gcccaaccgc cctgttttaa
     9961 cttcaggctt tcacgaattt cgttgagttc ttctcgcgct ttttccgacg tatcgcgcag
    10021 catacgcaac acttcttccg cctcaatatg acgccccttt tccgccagcc agcgcgggct
    10081 atttggcagg aagaccacca gaataatcag caggactgct ggtaaagcaa gaacccccaa
    10141 cattgcgcgc cagttaccgc tataactgaa cgctgtatcg gataaaaacg ccagcacgat
    10201 gccgagtgtg accatcaact ggtacatact gatcatctta ccgcgaacgt tttcacttgc
    10261 catttcagaa agatacagag gagcggtgta agacgcgatc ccgacagcaa tgcccagcac
    10321 cacccgagcg gcgattaaca tctctacgct ggtcgcaaaa gcggacccta tagaaccgag
    10381 tacaaacagg atggcccccg ccatcaggct gtatttacgc cccaggcgga acgacagcca
    10441 accattaaac agcgcaccaa ttgctgcgcc gagcatcatg ctactaacca cccattcctg
    10501 caaacgactg gtcagcacaa agtgatcggt aatgaacggc aacgctccgg cgattacgcc
    10561 gatatcaaga ccaaataaca atcctgcgac cgcagcagct accgaaacaa acatattcat
    10621 acgccgcgta tcccgcaaag aacgtggcgt taaagcagat tccgtattga tagtaaccat
    10681 tttttcctgc cagcagagag taagacatag tgaaaaaata cgtgaacaac tcacacaggt
    10741 gtcaggtcgg aaacagcata aatatggatt aaattgctgc gacatgtcgt tatgtgatgg
    10801 atattccaat tttcaaatta agttgaatta ttgagattat tattaaccac ctaattttac
    10861 agcagataaa attcataaag ttcattaatt gataattgat atggattatt tcataaccat
    10921 gatatgaatt atgatgacct acaggtataa aaaaccctgc catgaggcag ggtcataaaa
    10981 gtaagaggaa tgaattaacg agccagccaa ccgccatcta cggcaatggt ataaccattc
    11041 acataatctg aagcgctgga ggcaaggaac actatcggcc ccatcaggtc actcggcagt
    11101 ccccaacgac cagctggaat gcggtcgaga atttccgcgc tacgttgttc atctgcccgt
    11161 agttgttgag tattgttggt cgccatgtaa cccggagcta tcgcattaac attaatgttg
    11221 tgtttagccc attcgttcgc catcaatcgc gtcacgccca tcacgccgct ttttgatgcg
    11281 gtataagaag gcacacggat cccgccctgg aaggagagca ttgacgtgat attgataatc
    11341 ttgccgccat tgcgttgcgc gataaagtgt ttcgccgctg cctgagacat gaagaatacg
    11401 ctcttgatat tcaggttcat gacatcgtcc cagtcttttt cgctgaactc gagagcatct
    11461 tcgcggcgaa tcaatccggc gttattcacc aggatatcaa tatgaccaaa ctcagctacc
    11521 gcgcgatcca gcagtgctgg aatgccatca atctttcgca gatcggcggt caggcttaaa
    11581 aaacgacgcc ccagcgccgt gacctgcttg atggtttcag tcggttcaac gatgttaatg
    11641 ccaacaatgt cacagcccgc ttgcgccagc cccaacgcca tcccctggcc cagtccagta
    11701 tcacaaccag tgacgaccgc aactttacct tcgagagaaa atgcacttaa aatcataaca
    11761 ataccttatt cgtttatgcc gacaactagc gcaaatcttt aacggccact atgccgacaa
    11821 ctagcgcaaa tctttaacgg ccacatggtc catatcatca aagacctggt tttcaccaac
    11881 catgccccag ataaaggtat aagctttggt tccgacgccg gaatggatcg accagctggg
    11941 ggagatcacc gcctgctcgt tatgcatcac aatgtgacgc gtttcttgcg gctgccccat
    12001 catgtggaaa acgcaggcgt catcatccat attgaaatag aaataaactt ccatccggcg
    12061 ctcgtgagtg tgacacggca tggtgttcca caagttacct ggagccagct ccgtcagccc
    12121 catactcaat tggcaggttt ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg
    12181 gttactggtg aggttatcgc ctaacgtgac tggagatact tcgtccggtg tgactttttt
    12241 ggtgggatac gtcgtatgcg cgggtgcgca attgtaataa aactttgccg gagtggcggt
    12301 atcaatactg gcaaagacaa cttcttttgc acctttacca acatacaggg cgtcgcggtg
    12361 accgatttca tagcattggc catcgacagt aatcgtaccg gcaccgccaa tattgataac
    12421 acctaactcg cgacgttcaa ggaaatagct tacgccgagt tgtttaccaa cttccccgcc
    12481 aacggaaacc gttttagtta tcggcataat gccgccaaca ataattcggt caatgtggct
    12541 gtaaaccatg gtgtactcat cggcgacaaa taccttttca accaaaaatt cattgcgcag
    12601 cccttgggta tccagcgttt ttgcgtgcgc actgtggatg ccctgtctta cgtccacatc
    12661 aacctccgat aatcagtgaa gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt
    12721 tctctataaa gggatgatat gcccatgggc tagggttttc aattaaaaat aaaacaatgt
    12781 ttcattttta agttaaggat taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa
    12841 tggaaaaatg agggagccca gaaaatgtat gtcgacttgc cagccatcat tattcaacag
    12901 attaatgacg gcagatattt atcggtaagg aggaaatgca gagctactcg tcacgttcaa
    12961 tggtcaatgc cacgccctgg cccccgccga tacaaagcgt tgccagccct ttgcgggcat
    13021 tacgtttcac catttcatga accagagaaa ccaggattcg gcaaccggaa gcgcctatcg
    13081 ggtgaccgag tgcgatcgcg ccaccattga cattgacccg acgctcatcc cactcaagca
    13141 tcttgccaac cgaaagcgcc tgtgcagcaa acgcttcatt agcctcgata agatcgacat
    13201 cagccaactg ccagcctaca cgctccaggc aacggcgggt cgcatacacc ggcgcaattc
    13261 ccatcaatgc cggatctaca ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta
    13321 aattcaacgc tcgtgctttg gcttcgctca tcatcattac cgcagctgcg ccatcgttta
    13381 tggatgatgc attccccgcg gtcacagaac cgagactatc aaatgaagga tttaaacggg
    13441 ctaaaccttc tgcactggcg tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct
    13501 gaccgttact ttggatcatt accgggacga tctcatcttt aaatcgtccg gcgtcaatcg
    13561 ccgctcgcgc tttttgttgc gagctaagtg cgtaagcatc ctgcaactga cggctgatgc
    13621 catattcgcg agccagattt tcggcggtga caccaatatg ataatcattg aaggcatccc
    13681 acaacccatc atgcaccaga ctgtcaacca actggctatt gccaagctgt gcaccggtgc
    13741 ggctatcagt cagaacatgt ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa
    13801 tatcagcctc gccacactgt atcgcctgag tagccagatg cagtgcttta agtccggaac
    13861 cgcaaacgtc attaatcgtg attgcagaaa cgctattagg aagaccacct ttaatagccg
    13921 attgccttgc cggattctgc cctgcccctg cagtcaacac ctgaccaaga attacttcat
    13981 ccaccgcata tgcaggaacg ccggtacgtt ctattaacgc tttcacgacc agactaccaa
    14041 gttccacggc ggaatgaccc gctaacgcac cacgaaagca gccgataggt gtccgtaacg
    14101 ccccgacaat cacaacgtct ttcatcacaa cctcgcacca attaacagtg caatagtaaa
    14161 tgattgttaa ttacagttat cttaattgtt taaaaaaagt gatttttatc acaaaggaaa
    14221 tatgcctgag cagtagtcag agtcataact ggcacgtaag gtttgcaacc actaacccac
    14281 caatagatgg gtagataagg cgttaatctc ccatacttaa cctggtttat ggtaaattgc
    14341 cctccatttt gtttaatttg tagatgatac gttcagataa tgtctaatat ttggtctaaa
    14401 gaagaaactc tgtggagttt cgcgctctac ggcacagccg ttggtgcagg cacgctcttc
    14461 cttcctattc agttaggttc ggcaggggct gtggtcctgt ttattactgc tctggtcgcc
    14521 tggcctttaa catattggcc acataaagcc ttatgccagt tcatcctctc atcaaaaaca
    14581 tcagcatgtg aagggataac gggcgcggta acacactact atggcaagaa gattggtaat
    14641 ctgattacca cgctgtactt catcgccttt tttgtcgtcg tgttgatata tgcagtggca
    14701 attaccaact cacttacgga acagctggca aagcatatgg ttattgatct tcgcatccgt
    14761 atgttggtga gtctgggtgt tgtattaatt ctgaatctca tttttctgat gggacgtcat
    14821 gccactattc gggtaatggg atttttggta ttcccattga ttgcctattt cttatttctt
    14881 tccatttacc ttgtcggtag ttggcaacct gatctattaa cgacccaggt agagttcaat
    14941 cagaataccc ttcaccagat atggatatcg attcccgtga tggttttcgc ctttagccat
    15001 acgcccatta tttctacgtt tgccatagac agacgtgaaa aatatggcga acacgctatg
    15061 gataaatgca aaaaaattat gaaagtcgct tatctcatca tctgcataag tgtactgttc
    15121 tttgtcttta gctgcctgct ttctattcca ccttcgtata ttgaagcggc taaagaagaa
    15181 ggggtcacca ttttatcggc gctttctatg ctgccgaacg ccccagcatg gttgtcaatt
    15241 tccgggatta ttgtcgcagt agttgcgatg tcgaaatcat tcctgggtac gtactttggc
    15301 gttattgaag gtgccacgga ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa
    15361 agtcgtgcat ttaaccgcgc actatcaatt atgttggtat cgctgattac cttcattgtt
    15421 tgttgcatta acccgaacgc gatttcgatg atttatgcga tcagcggccc gctcattgcc
    15481 atgatacttt tcatcatgcc tacgttgtca acgtatctga tcccggcgct taaaccctgg
    15541 cgctccatcg gaaatctgat tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc
    15601 tttagctaat atccgttgtg gatggttcaa aattgctaca attttgaacc attccatttt
    15661 tcataatatt catttgttat tattccgccc tatcacttct ctcgtcatat cctagaatta
    15721 tcagttagtt aaacacacaa agagatctga acatcttaaa taagactatt taagatgcat
    15781 aacttagatt cgcaagatat cttctggtca ttcaaaaaca atttgcgata tagatattct
    15841 cataccatga gatagttatc tggaccacgt tcgaccatgc aagattaaat cagtgaaaca
    15901 taatcatatt taataacgag aaatgcattt ttaaatgtct taatcagaat tatagcaaat
    15961 acagattaat cgcaattttt cccagcaaaa ttacaaaatc aagatgtatt gacatcatca
    16021 atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt
    16081 agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa
    16141 gagtcaccgc gtttgcagta ttgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt
    16201 tcatatttta atcgtggtgt ggtggaaggt atcttaacaa aaaaccacaa tgcgagatta
    16261 agcggatata tttttgtgga tttttcagta agttttttac gtctattcct tgaaaaagac
    16321 tggattgact atcttgcaag tactgacatg gggatagttt tagtcagcga ccgcaatatg
    16381 caatcgttag ctaactactg gcgaaaacac aacaccgcaa tatctgctgt catatataat
    16441 gatgatggtc ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta
    16501 tcatttacca gaggtaacac attaactcag atggaattca ccataatggg gtatatggtt
    16561 tctggttata atccatatca gattgctgaa gtgctggata tggatatccg tagcatctac
    16621 gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt
    16681 tcacattcgg tccaacattg atactcaacg accagccaga atcatactct gttcatacgg
    16741 gcatctattg aaatgcccgt attttataaa taagcagtaa ccccaagagt aaacaacata
    16801 gtatttaaaa ccatcacacg aaaaattcag aagcattacg aaatatgaat ttacaaacaa
    16861 tagtggcata aatgttaacc atgttaattt acgtaaagtt ttacgttgca acattaaagc
    16921 ctcatttcaa tcatcatgat aaatataaaa ttaatatata tttatgccgt aaataccaat
    16981 atacttagca aactatgtga tctccatttc gattgattta gtgtttattg acgtatgtac
    17041 tggattatta acgataatat cgagttctgg cctgagcacc gaaaattaat atcggtacat
    17101 aacgccgatc ttaacgtcgt tctgacaacg ccagccagtc gatgtttatc acttctactt
    17161 gaagcttttc ctgatgtggt tacacaacaa gattttttca ccagagtctg ggaagaagaa
    17221 ggtatgcgtg tgcctactaa cacgttatat cagaacatat ccattatcag acgcggattt
    17281 cgcgctgttg gtgatactac ccactcgcta attgcaaccg tgccgagaga ggattcaa
//
LOCUS       DAZPEP010000081        17177 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZPEP010000081 DAZPEP010000000
VERSION     DAZPEP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17177
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.081"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            107..2335
                     /gene="crfC"
                     /locus_tag="ACHUT9_002598"
     CDS             107..2335
                     /gene="crfC"
                     /locus_tag="ACHUT9_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFR1349041.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2332..3198
                     /locus_tag="ACHUT9_002599"
     CDS             2332..3198
                     /locus_tag="ACHUT9_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1349042.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            3462..4964
                     /gene="proP"
                     /locus_tag="ACHUT9_002600"
     CDS             3462..4964
                     /gene="proP"
                     /locus_tag="ACHUT9_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFR1349043.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5076..5165
                     /gene="pmrR"
                     /locus_tag="ACHUT9_002601"
     CDS             5076..5165
                     /gene="pmrR"
                     /locus_tag="ACHUT9_002601"
                     /inference="COORDINATES: protein motif:HMM:NF033224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFR1349044.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5141..6241)
                     /gene="pmrB"
                     /locus_tag="ACHUT9_002602"
     CDS             complement(5141..6241)
                     /gene="pmrB"
                     /locus_tag="ACHUT9_002602"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFR1349045.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(6242..6910)
                     /gene="basR"
                     /locus_tag="ACHUT9_002603"
     CDS             complement(6242..6910)
                     /gene="basR"
                     /locus_tag="ACHUT9_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFR1349046.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6907..8550)
                     /gene="eptA"
                     /locus_tag="ACHUT9_002604"
     CDS             complement(6907..8550)
                     /gene="eptA"
                     /locus_tag="ACHUT9_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFR1349047.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            complement(8654..9991)
                     /gene="adiC"
                     /locus_tag="ACHUT9_002605"
     CDS             complement(8654..9991)
                     /gene="adiC"
                     /locus_tag="ACHUT9_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFR1349048.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10128..10889)
                     /gene="adiY"
                     /locus_tag="ACHUT9_002606"
     CDS             complement(10128..10889)
                     /gene="adiY"
                     /locus_tag="ACHUT9_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFR1349049.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11214..13481)
                     /gene="adiA"
                     /locus_tag="ACHUT9_002607"
     CDS             complement(11214..13481)
                     /gene="adiA"
                     /locus_tag="ACHUT9_002607"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFR1349050.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13680..14588)
                     /gene="melR"
                     /locus_tag="ACHUT9_002608"
     CDS             complement(13680..14588)
                     /gene="melR"
                     /locus_tag="ACHUT9_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFR1349051.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14871..16226
                     /gene="melA"
                     /locus_tag="ACHUT9_002609"
     CDS             14871..16226
                     /gene="melA"
                     /locus_tag="ACHUT9_002609"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFR1349052.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16329..>17177
                     /gene="melB"
                     /locus_tag="ACHUT9_002610"
     CDS             16329..>17177
                     /gene="melB"
                     /locus_tag="ACHUT9_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFR1349053.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
BASE COUNT         4130 a         4148 c         4498 g         4401 t
ORIGIN      
        1 taaatataaa attatttctt gcagtaatta taaaagccaa aaacagaact caaaaacaat
       61 ctggctaaat aaaagtaaca aaatttgctt taaggaagaa ttttctatgt acacacagac
      121 cctgtatgag ttaagtcagg aggctgaacg cctgttacag ctttctcgcc aacagttgca
      181 gttactggaa aaaatgcctc tctctgtacc cggagacgac gcgccacaac tggctttacc
      241 ctggagtcag cctaatatcg ccgaacgtca cgcgatgctg aataatgagt tgagtaaaat
      301 ttcccgactg gaaatggtgc tggcgattgt cggtactatg aaagcaggga aatcaaccac
      361 cattaatgcc attgttggta cggaggttct gcctaatcgt aatcgcccaa tgactgcgct
      421 gccgacgctt attcgccata cgcccgggca aaaggaaccg gtactgcatt tttcacatgt
      481 cgcgccaatc gattgtttaa ttcaacaatt acaacagcgc ctgcgtgatt gcgatattaa
      541 gcatctgacc gatgtgctgg aaatagataa agatatgcgt gcgcttatgc agcggatcga
      601 aaatggcgtc gctttcgaaa aatattatct gggtgcccag cctatttttc attgtctgaa
      661 aagtttgaat gatttagtgc gactggcgaa ggcgctggac gtcgattttc ctttttctgc
      721 ttacgccgcc attgagcata ttcccgtgat tgaagtggag tttgtccatc tggcggggct
      781 ggagagttat cccggtcagt tgacgttact ggataccccc gggccaaatg aagccgggca
      841 accgcatctg caaaaaatgc ttaaccagca gctggcacgc gcctcggcgg tactggcggt
      901 gctggattat acgcaactga aatcgatctc cgatgaagag gtccgtgagg cgattttggc
      961 ggtggggcaa tcggtgccac tgtatgtgct ggtcaataag ttcgatcaac aggatcgtaa
     1021 cagtgacgac gccgaccagg tgcgggcact gatttccggg acgctgatga aaggctgtat
     1081 tacgccacag cagatatttc cggtgtcgtc gatgtggggc tacttggcaa atcgagcgcg
     1141 ccatgagtta gccaacaacg gtaagttacc agcgccagag caacaacgct gggtggaaga
     1201 ttttgcccat gccgcgctcg gcaggcgctg gcgtcatgcc gatctggcgg acctcgaaca
     1261 tattcgtcat gctgccgatc agttgtggga agattcgctg ttcgcccagc caattcaggc
     1321 gttgcttcat gccgcttacg ctaacgcctc gttgtatgct ctgcgatctg ctgcgcataa
     1381 actgttgaat tacgcgcagc aggcgcggga atacctggat tttcgtgcgc acgggttaaa
     1441 cgtcgcttgt gaacaattgc ggcaaaatat ccatcaggtc gaagaaagtt tgcagctatt
     1501 gcaactcaat caggcgcagg tgagcggcga gattaaacat gaaatcgagc tggccctgac
     1561 ctccgccaac cactttctgc gtcaacagca agatgcgctg aatgcgcagt tagccgcctt
     1621 gtttcaggat gattcggagc cgttaagcga gattcgtacc tgctgtgaga cactgttaca
     1681 gacggcgcag aacaccatca gtcgcgactt tacgctgcgt tttgccgagc ttgaatccac
     1741 cctttgccgg gtgttaaccg atgttattcg gcccattgag caacaagtca aaatggaatt
     1801 gagcgagtca gggtttcgtc ctgggtttca ttttcctgtt tttcacggag tagttcccca
     1861 cgtcaacact cgccagctgt tcagtgaagt catttcgcgc caggatgcaa cggacgagca
     1921 gagcacgcgt ttaggcgttg tgcgtgagac tttttcgcgc tggttgaatc agcccgactg
     1981 gggacgggga aatgagaaat ccccgacaga gacggttgat tacagtgtgt tgcaacgagc
     2041 tttaagcgca gaagtcgatc tttattgcca acaaatggct aaagttctgg cagagcaggt
     2101 cgatgaatct gttacggcag gcatgaatac tttttgcgct gagttcgctt catgtttgac
     2161 ggaattacag acgcgtttac gcgaaagcct ggctctgcgt caacaaaatg aatcggtggt
     2221 caggctgatg cagcagcaat tgcagcagac tgtgatgact cacggctgga tttacaccga
     2281 cgcccagctg ttacgcgatg atattcaaac acttttcacg gcagaacgat attgaccaag
     2341 acgttactgg acggccccgg tcgcgtgctg gagtcggttt atccccgctt tttagtggat
     2401 ctggcgcagg gcgatgatgc ccgccttcca caagcccatc agcagcagtt tcgtgaacga
     2461 ctgatgcagg aacttctttc gcgtgtgcag cttcagacat ggacgaacgg cggcatgtta
     2521 aatgcgccgc ttagcctgcg actgacaatg gtggaaaaac tggcgtcgat gctggatccc
     2581 ggtcatctgg cgctgacgca gatcgcgcag catctggcgc tgctgcaaaa aatggatcac
     2641 cgccagcact ctgctttccc ggagctcccc cagcaaattg ccgccttgta tgagtggttt
     2701 tcagcccgtt gtcgctggaa ggaaaaggcg ttaacgcaac gaggcctact ggtgcaggca
     2761 ggtgatcaga gcgagcaaat ttttacccgc tggcgtgctg gggcgtataa cgcctggtcg
     2821 ttgcctgggc gctgttttat cgttctggag gagttgcgct ggggtgcatt tggcgatgcc
     2881 tgccgtctgg gaagcccgca agcggtggtg ttgttactgg gtgatttgcg cgagaaagcg
     2941 acacaacatc tggcagagag tatcaatgcg gcaccgacca cgcgtcacta ttaccatcag
     3001 tggtttgcct cttcgggagg ggagcatgct gattttttaa gttggctggg aaagtggtcc
     3061 acggcagata aacagcccgt ttgctggtca gtgacccaac gctggcaaac agtcgcgctg
     3121 gggatgccac gactctgttc agcgcagcgt ttggtggggg caatggtcga ggaaatcttc
     3181 tctgtaaatt tggcgtaaat aatcagttac atcaatgagt cctaaacgaa atccatgtgt
     3241 gaagttgatc acaaatttaa acactggtag ggtaaaaagg tcattaactg cccaattcag
     3301 gcgtcaactg gtttgattgt acattcctta accggagggt gtaagcaaac ccgctacgct
     3361 tgttacagag attgcatcct gcaattcccg ctcccctttt gcggccgtcg cgctgatttt
     3421 tctggcgttt gcggaaatgg gccaactctg cgaggaaagc tatgctgaaa aggaaaaaag
     3481 taaaaccgat tacccttcgt gatgtcacca ttattgatga cggtaaactg cgtaaagcca
     3541 ttaccgcagc atcactgggt aatgcaatgg aatggttcga ttttggtgtt tatggttttg
     3601 ttgcttacgc attaggtaaa gtttttttcc cgggggctga ccccagcgtg cagatggttg
     3661 ctgcacttgc cactttctcc gttccctttc tgattcgacc gcttggcggg ctcttctttg
     3721 gtatgttggg cgataaatat ggtcgccaga agatcctcgc tatcactatt gtgattatgt
     3781 cgatcagtac gttctgtatt ggcttaatac cgtcctacga cacgattggt atttgggcac
     3841 cgattctgct gttgatctgt aagatggcac aaggtttctc ggtcggcggt gaatataccg
     3901 gggcgtcgat atttgttgcg gaatactccc ctgaccgtaa acgtggcttt atgggcagct
     3961 ggctggactt cggttctatt gccgggtttg tgctgggtgc gggcgtggtg gtgttaattt
     4021 cgaccattgt cggcgaagcg aacttcctcg actggggctg gcgtattccg ttctttattg
     4081 ctctgccgtt agggattatc gggctttacc tgcgccatgc gctggaagaa actccggcgt
     4141 tccagcagca tgttgataaa ctggaacagg gcgaccgcga aggtttgcag gatggcccga
     4201 aagtctcgtt taaagagatt gccactaaat actggcgcag cctgttgaca tgtattggtc
     4261 tggtaattgc caccaacgtg acttactaca tgttgctgac ctatatgccg agttatttgt
     4321 cgcataacct gcattactcc gaagaccacg gggtgctgat tattatcgcc attatgatcg
     4381 gtatgctgtt tgtccagccg gtgatgggct tgctgagtga ccgttttggc cgtcgtccgt
     4441 ttgtgctact tggtagtgtt gccctgtttg tgttggcgat cccggcgttt attctgatta
     4501 acagtaacgt catcggcctg atttttgccg ggttactgat gctggcggtg atccttaact
     4561 gctttacggg cgttatggct tctaccttgc cagcgatgtt cccgacgcat atccgttaca
     4621 gcgcgctggc ggcggcattt aatatttcgg tgctggttgc cggtctgacg ccaacactgg
     4681 cggcctggct ggtcgaaagc tcgcagaatc tgatgatgcc tgcctattac ctgatggtag
     4741 tggcggtgat tggtttaatc accggcgtaa ccatgaaaga gacggcaaat cgtccgttga
     4801 aaggtgcgac accggcggcg tcagatatac aggaagcgaa ggaaattctc gtcgagcatt
     4861 acgataatat cgagcagaaa atcgatgata ttgaccacga gattgccgat ttgcaggcga
     4921 aacgtacccg cctggtgcag caacatccgc gaattgatga ataagctgaa acggatggcc
     4981 tgatgtgacg ctgtcttatc aggccaattg aactcttaag gttcacttaa tctctgacgc
     5041 gcatactctc ctccaggtta acggaggaga gtgcaatgaa aaaccgtgtt tatgaaagtt
     5101 taactaccgt gttcagtgtg ctggtggtca gcagctttct ttatatctgg tttgctacgt
     5161 actgatcttt cttcagccgt acccaggccc gcgtgccgga agtctcttgc cggttttgca
     5221 ggaaaaactg cccgtgatgc aactgtgtaa tgcggctgac aatacttaac cccagaccaa
     5281 tcccgccata acggctgtcc atacgtacaa acgctttact caactccccg catttactct
     5341 catcaatacc cggtccttca tcttcaacgg ccatgaccgc tccgccatct tcttgcagct
     5401 taatcataat gttgctgcct tgtgggctgt aacgatgggc gttttctacc aggtttcgca
     5461 ataacatccg cagcagggtt gcatcaccct gaacggtgat gtcggcggcg ctctctggca
     5521 atagcagggt ttgctgtcgc tggtcgagca tggtactgag ttcgtcatac gaggggagaa
     5581 tgacatcttc cagcagtttt acatgttgat aattaccgga agaaaatgac tgtccggcac
     5641 gcgccagttg cagcagctgg gagacgctct ccatcatctg atcaagccgt gccactaacg
     5701 gtgctacatc aatgtgatgc gttttcgcca gcagttccag atgcaaacgc acccccgcca
     5761 gtggcgttcg cagttcgtgc gcgacgtcag cggtaaataa cctttcgtta tccagcgtgc
     5821 tcgtcaggcg actgaccaga tcgttcaacg ccgaaaccac cgcttcgatt tcgagggtgg
     5881 cgctgtgaat ggcaatgggc gttaagttgt cggcggtgcg cgcttccagc tctttttgca
     5941 gctccgccag cgggcgggtg atgcggcgta ccgcctgata gcagataaat agcgtcaggc
     6001 tgaccataaa gacgccgggg acaatcaggc tggcgaccgc ctcgcggatc tcacgcatga
     6061 tgtggcgatc gttgttgcga ttgtcgcgta gcgcctgctc aaacagctga atctgctcgg
     6121 tactttcatg ccatagccag aagacgctga tcagctcaaa caccaacaaa atggccccga
     6181 tggtcaatat cagccgttgg cgcagcgata ttggtcggcg cagaaaacgc atcagattca
     6241 attagttttc ctcattcgcg accagcatat agccaaagcc gcgcacggtg cggatacggg
     6301 ctttgcccac tttgtcgcgc agattgtgga tatgcacttc cagggtgttg gtcgagggtt
     6361 cattgtccca gttatagatg tcgttgtaga gaatttcccg atgtaccgga ctgcctgctt
     6421 tgagcattaa ccgtgacagc agagcatatt ctttgggcgt cagaatcaac tcttcaccgc
     6481 ccatccatac ctgacggcga cccatattca gcgtcagatt gccaacaatc agctcacttt
     6541 cgccctgatt attatggcgt cgtagcaggg cgcggatacg ggcgtgtaac tcttccagcg
     6601 caaaaggctt caccagatag tcatcggcac cgacatccag cccggcgatt ttgtcggtca
     6661 gcgtatcgcg agcggtgagg atcagtaccg gcagggtgta ttttttctgc cggatacggg
     6721 cgagaaaatg cagtccatct tcgtcgggta accctaaatc cagtaccacc aggctgtaat
     6781 gaccggcctc aaggctttgt tccgccatcc gcgcggttgt tacgccatcg cacgtgtagc
     6841 cttcggtttg cgccgccaga atcagtccct gcaataacag cgtatcgtct tcaacaatca
     6901 gaattttcat tcactcactc tcctgcaagt ttgcagaaca tcatccgcag cctggtaata
     6961 cttcgtctca acgccagtta atcccaatag cgtggagaat aaattgtctt gtgaatagtg
     7021 ttgcgtttgc gcctgttttt gcaggcagtt ctggtcaacc tgataccgtt tttgataatc
     7081 ctccgacagc cacagcagca tcggcacctg tttttggcta tccggggcga tggcataagg
     7141 cagaccgtgc agatagatgc cattttcacc taacgattca ccgtggtcag aaagataaac
     7201 caggctggtg gtaaatttat cctgatgttc tttcagcaga ttaatcgctt tatcaacaat
     7261 atagtcgacg taaaccagcg tgttgtcgta agtgttcacc agttgctctt tggtacaggt
     7321 ctggatctca ttggtgtcgc aggttggggt aaatttcctg aactgcggcg gatagcggtt
     7381 gtaataggtc ggaccgtggc tgccgatggt gtgtaagaca atcacgccat caccctgcag
     7441 gttattgatg tactcttcca gcccgtggaa cagcacttcg tcatagcatt cgccgttgat
     7501 gcactgacca ggcagattca gcgcggtgac gttctggtga ggcacgcggt cgcaggcacc
     7561 tttacagccg ccatcgttgt cattccacag cacgttgatg cccgcttgct gaatgatatc
     7621 cagcacgcct tcctggtgct gtgccagctc ttctttgtaa tgctcacgcg gcatatccga
     7681 gaacatgcac ggtactgaaa ctgccgttgc cgtgccgcaa gatgcggtat tagggaaata
     7741 gaccacgtta tctttcgcca gccgcgggtt agtttcacgc gggtagccgt tgagggagaa
     7801 gttctccgcc cgcgaggttt cgccgacaat caggatggtc aaatttttac gtttttcgtt
     7861 ctgcattaac gggttgcggt gggcgtcttc accaattcgc accagcggta gatttgccag
     7921 tcgctgatgg gagtaccatg accagctggc aacaatgctg ttagaggggc ttaaggattt
     7981 caccagctct ttgttattgc ggaacaacga ggcgtagtct ttataaaaca gtgcggcgac
     8041 cagcaaaatc agcagtacag aaaccagaat attggctcca cggaaaagaa cactgcgcag
     8101 acgcgaggtg gcaggtttga tttttagcca gcaggcaatc agcgcagcaa gcacgccgct
     8161 gaatcccagc gttaataaca tttgcggtgt catcagcgca taactttctg ctggagtggt
     8221 atcaataata ttggcaatca tcgagcggtc gatgacgatg ccgtaagtca ttatgaaata
     8281 ttgtgcagcc gcgccaacca gaataaacag gcaggccagt ggtcgattaa gccataagaa
     8341 agagcttagt gtcaggacaa tattaatcac gctgaaagcg acgaccggca tcgacaagaa
     8401 aaccagtacg ttatgcagcg aatccagcgg cagcgcctgc aacacctgtt taaaaaaggc
     8461 aatattcagg cagatagaga tataaaaagc ggccaacaat agccaggcga gtaaattcaa
     8521 agaggggctt tttagtaggc gcttcaacat cacggtgttt ccatcgaaca aagtgcgcat
     8581 atgctcgcaa agcaaaatta agccaacctt aagttcttaa ggttggcttt tatgtttgct
     8641 ggattaaggg gaattaatct ttgcttattg gtgcatctaa gggatacggg tttttatgta
     8701 gccggttgta attcagcgca tacatggcgg tgatgaccat cagggtgaca aatgaccaca
     8761 taacctcttt cgccccggac cccaccacgg cccagatgca gtagaggaag gcaatggtag
     8821 taacagccag atatgccggg cgtgctttac caaagtgacc gtggccgagc agcagtaacg
     8881 ccgcacaggt gtaaagatat ggcaccagtg taaagatgac cgacacggaa gataccagac
     8941 cgaactcttt ggttgcgttt ggcgaaatgc tactgagctg gaagatggtc atcaaaatac
     9001 cgacgataat cagccccgcc actggcgtac ccgctttatt tacacgggca aaaatcggtg
     9061 ggaacagtcc gtcatcggca gcggctttcg ccgtttgacc cgccagcaac gtccagccgc
     9121 ccagtgaacc taagcaaccc gcagctgcgc agaaggaaac aatggcccca gcggtgtcac
     9181 ccaacgccat ccgtgcggca tcaccgaatg gcgaggcaga aacgcgcagt gcggcattag
     9241 ggatcatccc cataatcgcg gtggtggaga gcacatagca aacggcggca atcaataccc
     9301 caccaatggt ggcgataggg acattgcgtt tcgggttttt caccacacct gcggcaacgg
     9361 aggcactttc cacaccgatg aacgaccaca gcgtaacgtt aagggtactt tgaatcgcac
     9421 cgaaggtgcc caggccgctg acgttccatg ccgccatata ggtttcacca cggaaccaga
     9481 accagccaaa tacggcaatc ccgacgatgg ggatcagcgc cagcacggtg gcaacggcct
     9541 gcacacgggt gatcattttc ggaccgacaa tgttcagcag gacgaagatc cacagcacca
     9601 cgacgcaggt gatggttaat accaatggat ctttcagaat cgggaagaag taacttaaat
     9661 atcctacgcc aatgaccacc atagcgatat tgccgatcca gcaggccagc cagtagagga
     9721 cgttggtttg ataaccgaga aacgggccaa agcagcggcg ggcgtaagcg taagaaccac
     9781 caggacttgg atcgaggaac gacattttgg cgtataccat cgagagcccc agcgcaccga
     9841 taatcgtcac caaccatcca taaatggcaa tcccgccagt ggaggccagg tttgcaggta
     9901 acagaaaaac acctgacccc ataatattcc ccgacaccat cagggtgacg gggattaagc
     9961 ccactttgtg agcatcagca tccgaagaca taattaaact cctgcgaagg cgagcttcgt
    10021 gacaataaat tacgtcatat catacgcctg cattcgatag ttgaattatt atcgaccggg
    10081 ttatcgctga tgccgttatt aatatagctc ttcgttaagg ttaaaaatca ggcgacagtt
    10141 cgttctctgt gctgactaac ataatgcagc ggcgtcatac cgtagaagtc tttaaagaca
    10201 gaaataaagt acgacgtact gttgtagccg caggactgtg atacctgaga gatattttta
    10261 ccgtccatca ttaattcatt tacggcataa cgcatgcggc aggtggtgat tatttggcta
    10321 taactggtgt tttcgctttt cagctttttc tttaacagac ttgggctaag acataaacaa
    10381 ctggctacca tactaagatt ccagtctttg tgaatatcgc tttcaataat ttgataaacg
    10441 ctgtcactta cacaattacg taacatatac atcattaatg aaaggaattt tttactgtcg
    10501 agaaaacggg atagcaccgt gaataataat gcgcgtgttc gttccttttc tgactcagtt
    10561 tctgcgggca tcatgctgtg ttgcgcggca acgcgaaaga cgtctggcgt caggcatggc
    10621 agggtgagta tgggcgtagc gctacgttgc catactggta tttgcgagag atctttattc
    10681 agaaaccgga ggtaatcttt gatgatgtcg tgactaatat gagcaaccaa agtgttgtta
    10741 agagaggaga cgtcgataat attattttca caatttaata acgccatatg gttagcttta
    10801 aggctaatag gttctttccc attcaccctt atccagacat ctttttcagt caataaaaca
    10861 atacaaggtt ggtcgctgca aatcctcata catgtactcc tgagtgcgaa taaaagacca
    10921 gttctcgcta aagcaaagcg ataccgggaa atggttatat aattgtatga tgaatataaa
    10981 catgcataaa aataacgagt tttactaata ttacaaattt ttatagtaat ttctcatgta
    11041 catatttttt tacaggataa ataacaggca aaaaaaagcg cggggtgggg ccgcgcaaaa
    11101 agtatgacat ttttgtttgc agcaattttt atgaaaacgt tgccatactc aaaaggccta
    11161 aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc agcggaatag tggttacgct
    11221 ttcacacaca taacgtggta aataccgtca ataatttcag tcccttcagt ttcgtgttca
    11281 aatccaggga aatggtggtc ccaggattgc agcgagcgta aataacttac ttgcggactg
    11341 tttttatcgc cgaagttttc accagacagc agcatcggga ttcctggcgg atatgggata
    11401 actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg ataccagttc gacattgttg
    11461 tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg ccaccgacag gccggaatag
    11521 gcttcgttca accgtgcgcc agggttgttt tctttcagcc aggcaaacat ggtgtcaccc
    11581 agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt gttcaacaag ttccggcatc
    11641 acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga aggagcaaag ggtgttaacc
    11701 agagttcccc atttcccacg ggttacgccc atagagaaca ggaacataat ttggaagtca
    11761 gtggtgcggg taggcacaat gccgtggcga ccaagccagg cagtgaccag cgccgccgga
    11821 acaccggttt cttccagttc accatcttca cccattcccg gagcaaggat gctgacttta
    11881 atcgggtcga gcatactcca gttatccgga atatctttga agccgtgcca gctttcgccc
    11941 ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt tggttggtgc gtcagcaaag
    12001 tcataggttt tgccggtttg tgggtcggtg acgacttctt tgttccacgg tttgaagaac
    12061 cagctaccgt cagcggtgaa ctctttatac agccgcgcca tcgcctgacg gaaatcaacc
    12121 gcttcgtcga tcacttcctg cgtcagtgac aggccgctgt tgccgtccat catcgacacc
    12181 gccacgtcgt tggatgcgca gatggcatac agcggggagg tggtggcatg catcatgtag
    12241 gcctggttga agcgggagaa gttaatcgcc ccacgacctt cacgtacatg aatataagaa
    12301 gcctgtgaaa gcgcattcag cagtttgtgg gtggagtggg tggcgaaaac ggtaggaccg
    12361 ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg catagatcgg gttgaaacgt
    12421 gcatagccgt accaggcttc gtcaaagtgc agacgatcgg aggttttttc cagcagatcc
    12481 tgcgcttctt tagcgttata acacacgccg tcataggtgc agttggtcac cacgcagtaa
    12541 gacggttttt gcccggcttt gtctttagtc agcgggcttt cactgatttt cttctgcaag
    12601 gtttcaggtt gcatttcctg cggatagatt ggcccgataa tgccgtagcg gttgcggctt
    12661 ggcaccatgt agaccggttt cgcgcctgtc agcatcaagc cttgttcgat ggatttatgg
    12721 cagttacggt caacgaccac gacatcgtta tcggtcatgc aagcctgcat gatggtgcgg
    12781 ttagagccgg aagtaccgac gactaccgac caggagcgat cggcaccaaa tacgcgtgcg
    12841 gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa gcaaagaacc gagggaagtt
    12901 cgttcgatgc ccatatcggt gcggaacaga ttttcaccat agtagtcatg gtagaaacgt
    12961 ccggcgggtg ttttggtaaa accaacgccg ccctggtggc ctggcgctgc ccaggaatat
    13021 tcatggatgt cgctatattt catcagcgcg ctgaacagtg gcggcaacag ctgctggcgg
    13081 tagcgggtca tcgcggcaac agcgcgtccg gcgataaagt cggcggtatc ttccagaatc
    13141 caggcgaatt catcgacaag ctccagcagg tcgcgatcca ttgcggcgag ggctttttcc
    13201 cgatcgccca acaggaagac cggcacgttt tgttggcgct catgaagctt accgatcaat
    13261 tgtctgacgt tttgatgttc gtccggatgt tccatttgat agctgaacat caggcagtca
    13321 atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa aggaggtgga tttaatcacg
    13381 gtaacatttt gctggcttaa agcatctgcc agacgctcaa cggcgttacc gacccaggtg
    13441 tcttgatgga gaaactcgct ttcaacaatt aatactttca tcattgctta cccggttatg
    13501 aaggaatctt cgttgcgggc aagtatcttc cggccttttc gggctgtaaa gcgcgtgaaa
    13561 aacaaacatt tttgaaatat ttatttttat aaacaatgcg ttatgtgatt attttaaatt
    13621 attttcactg cttattatcg acggctaaac tatttttttg gctgatactg atatcgtctt
    13681 tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc ggtattgttg cggcgacatg
    13741 ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg aacgaaagcc tgccgtcagg
    13801 gcaatatcga gaatactttt atcggtatcg ctcagtaacg cgcgaacgtg gttgatgcgc
    13861 atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc gctggaatat ccccattgca
    13921 tagttggcgt taagtttgac gtgctcagcc acatcgttga tggtcagcgc ctgatcatag
    13981 ttttcggcaa taaagcccag catctggcta acataaaatt gtgcatggcg cgagacgctg
    14041 tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt cccagccaga gaggctaaat
    14101 cgcttgagca tcaggccaat ttcatcaatg gcgagctggc gaatttgctc gttcggactg
    14161 tttaattcct gctgccagcg gcgcacttca aacgggctaa gttgctgtgt ggccagtgat
    14221 ttgatcacca tgccgtgagt gacgtggtta atcaggtctt tatccagcgg ccaggagaga
    14281 aacagatgca tcggcagatt aaaaatcgcc atgctctgac aggttccggt atctgttagt
    14341 tggtgcggtg tacaggccca gaacagcgtg atattaccct gattgatatt cactttttca
    14401 ttgttgatca ggtattccac atcgccatcg aaaggcacat tcacttcgac ttgaccatgc
    14461 cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa tctccatccg ctggtattcc
    14521 gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc tgctgcacat aaacgtatct
    14581 gtattcatgg atggctctct ttcctggaat atcagaatta tggcaggagt gagggaggat
    14641 gactgcgagt gggagcacgg ttttcaccct cttcccagag aggcgagggg agcgtccgag
    14701 catcatgagg ccgaaaactc tgcttttcag gtaacttatt accataaact cagatttact
    14761 gctgcttcac gcaggatctg agtttatggg aatgctcaac ctggaagccg gaggttttct
    14821 gcagattcgc ctgccatgat gaagttattc aagcaagcca ggagatctgc atgatgactg
    14881 cacccaaaat tacatttatc ggcgctggtt cgacgatttt cgttaaaaat attcttggtg
    14941 atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc cctgatggac attgatccca
    15001 cccgcctgga agagtcgcat attgtggtgc gtaagctgat ggattcagca ggggccagcg
    15061 gcaaaatcac ctgccacacc caacagaaag aagccttaca ggatgccgat tttgtcgtgg
    15121 tggcatttca gattggcggt tatgaacctt gcacggtgac tgatttcgag gtctgtaagc
    15181 ggcatggtct ggaacaaacc attgccgata cgttggggcc gggcggtatt atgcgcgcgc
    15241 tacgtaccat tccgcatctg tggcaaattt gcgaggacat gacggaagtc tgccccgatg
    15301 ccaccatgct caactacgtt aacccaatgg cgatgaatac ctgggcgatg tatgcccgct
    15361 atccgcatat caaacaggta gggctgtgcc attcggtgca gggaacggcg gaagagctgg
    15421 cgcgtgacct caatatcgat ccgtccacgc tgcgttaccg ttgcgctggt atcaaccata
    15481 tggcgtttta cctggaactg gagcgtaaaa ccgccgacgg cagttacgtg aatctctacc
    15541 cggaactgct ggcggcttat gaagcagggc aggcaccgaa gccaaatatt catggtaata
    15601 ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa gctgggctac ttcgttacgg
    15661 aatcgtcaga acattttgct gaatacacgc cgtggtttat taagccaggt cgtgaggatt
    15721 tgattgagcg ttataaagta ccgctggatg agtacccgaa acgctgcgtc gagcagctgg
    15781 cgaactggca taaagagctg gaggagtata aaaacgcctc ccggattgat attaaaccgt
    15841 cacgagaata tgccagcaca atcatgaacg ctatctggac tggcgagccg agtgtgattt
    15901 acggcaacgt ccgtaacgat ggtttgattg ataacctgcc acaaggatgt tgcgtggaag
    15961 tagcctgtct ggttgatgct aatggcattc agccgactaa agtcggtacg ctaccttcgc
    16021 atctggccgc cctgatgcaa accaacatca atgtacagac gctgctgacc gaagctattc
    16081 ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat ggacccgcat actgccgccg
    16141 tgctgggcat tgacgaaata tatgctcttg ttgacgacct gattgccgcc cacggcgact
    16201 ggctgccagg ctggttgcac cgttaaaacg cgactaaacg ctactgcgcc gggggattta
    16261 ttccggcgca cacctctgac gataccaata acagaaggcg ggcgttggta acagcgaccc
    16321 gataccctat gagcatttca atgactacaa aactcagtta tggatttgga gcgttcggga
    16381 aggattttgc gatcggcatt gtgtatatgt acctcatgta ttactacacc gatgtcgttg
    16441 ggctgtctgt gggtttggtc ggtactttgt ttctggtggc gaggatctgg gatgctatta
    16501 acgatccgat tatgggatgg attgtaaatg ctacgcgatc gcgatggggt aagttcaaac
    16561 cctggatcct gatcggtacg ttggcaaact ctgtaatctt atttctcctc tttagtgcgc
    16621 atctgtttga aggtactact cagattgtct ttgtttgcgt gacctacatc ctctggggca
    16681 tgacttacac cattatggat attcccttct ggtcgctggt tccaaccatc acgctcgata
    16741 aacgtgagcg tgaacaactg gttccttatc cgcgtttttt tgccagtctg gcgggctttg
    16801 ttacggcagg tgtgacgcta ccatttgtta attatgtcgg cggtggcgat cggggatttg
    16861 gctttcagat gttcacactg gtactgatcg ccttttttat tgtttcaacc atcatcactc
    16921 tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc gtctgctgaa ggaagccatc
    16981 tgacacttaa agccatcgtt gcgctaattt ataaaaacga tcagctttca tgcctcttgg
    17041 gtatggctct tgcttataat gtagccagca acattattac cggatttgct atctattatt
    17101 tctcatatgt tatcggtgat gcggatttgt tcccctatta tctgtcgtat gcgggagctg
    17161 ctaacctggt gacgtta
//
LOCUS       DAZPEP010000082        16583 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZPEP010000082 DAZPEP010000000
VERSION     DAZPEP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.082"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(38..2635)
                     /gene="topA"
                     /locus_tag="ACHUT9_002611"
     CDS             complement(38..2635)
                     /gene="topA"
                     /locus_tag="ACHUT9_002611"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFR1349054.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3015..3266
                     /locus_tag="ACHUT9_002612"
     CDS             3015..3266
                     /locus_tag="ACHUT9_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFR1349055.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3302..4351)
                     /gene="sohB"
                     /locus_tag="ACHUT9_002613"
     CDS             complement(3302..4351)
                     /gene="sohB"
                     /locus_tag="ACHUT9_002613"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFR1349056.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4571..5329
                     /locus_tag="ACHUT9_002614"
     CDS             4571..5329
                     /locus_tag="ACHUT9_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFR1349057.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5326..5916
                     /gene="cobO"
                     /locus_tag="ACHUT9_002615"
     CDS             5326..5916
                     /gene="cobO"
                     /locus_tag="ACHUT9_002615"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFR1349058.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(5956..6828)
                     /gene="rluB"
                     /locus_tag="ACHUT9_002616"
     CDS             complement(5956..6828)
                     /gene="rluB"
                     /locus_tag="ACHUT9_002616"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFR1349059.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7041..8155)
                     /locus_tag="ACHUT9_002617"
                     /pseudo
     CDS             complement(7041..8155)
                     /locus_tag="ACHUT9_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(8183..8803)
                     /locus_tag="ACHUT9_002618"
     CDS             complement(8183..8803)
                     /locus_tag="ACHUT9_002618"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFR1349060.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(8800..9681)
                     /gene="rnm"
                     /locus_tag="ACHUT9_002619"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             complement(8800..9681)
                     /gene="rnm"
                     /locus_tag="ACHUT9_002619"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFR1349061.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            9819..9863
                     /gene="trpL"
                     /locus_tag="ACHUT9_002620"
     CDS             9819..9863
                     /gene="trpL"
                     /locus_tag="ACHUT9_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFR1349062.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    9840..9933
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            9955..11517
                     /gene="trpE"
                     /locus_tag="ACHUT9_002621"
     CDS             9955..11517
                     /gene="trpE"
                     /locus_tag="ACHUT9_002621"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFR1349063.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            11517..13112
                     /gene="trpD"
                     /locus_tag="ACHUT9_002622"
     CDS             11517..13112
                     /gene="trpD"
                     /locus_tag="ACHUT9_002622"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFR1349064.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13113..14474
                     /gene="trpCF"
                     /locus_tag="ACHUT9_002623"
     CDS             13113..14474
                     /gene="trpCF"
                     /locus_tag="ACHUT9_002623"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFR1349065.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            14486..15679
                     /gene="trpB"
                     /locus_tag="ACHUT9_002624"
     CDS             14486..15679
                     /gene="trpB"
                     /locus_tag="ACHUT9_002624"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFR1349066.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            15679..16485
                     /gene="trpA"
                     /locus_tag="ACHUT9_002625"
     CDS             15679..16485
                     /gene="trpA"
                     /locus_tag="ACHUT9_002625"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFR1349067.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         3924 a         4446 c         4174 g         4039 t
ORIGIN      
        1 cgaccctttg tttataaaaa cctgacagaa ttaaaggtta tttttttcct tcaacccatt
       61 tgccatcaac ataaaatgct gaccagccag tcgcctttcc gtctttttcc gaagagacat
      121 attgctgttt ggttttacgg ctaaagcgaa ccatggtctt attaccttcc ggatcctgct
      181 gtggcgcatc ggccagataa cgcagttttt ccggcagacg gtcgcggaag cgataaagct
      241 cttccaccag tggcgcacgc gtttcacgcg atttcgggaa agtgttggca gccaggaaaa
      301 cacccgcagc gccgtcacgc agcacgaaat aagcatctga tttttcgcac ggcagctcag
      361 gtaatggcac cggatcttct ttcggtggtg ccacttcgcc gttacgtaaa atcttacgtg
      421 tgtttttaca ctcttcgttg gtgcaggcca tgtatttacc gaaacgcccc attttcaggt
      481 gcatttcaga gccacatttt tcacactcaa cgatcgggcc gtcataacct ttaatgcgga
      541 attcgccctc ttcgatctcg taaccgtcgc aggttgggtt attaccacag acatgcaact
      601 tacgtttcgg atcgatgaga tagctgtcca tcgccgtgcc gcatttcggg caacgacgtt
      661 ttgcgcgcag cgcgttggtt tcagcgtctt cgccttccag tacgttcagc acttcgtttt
      721 ccggcaccag gttaatggtg gttttgcaac gctctttcgg cggcagcgca taaccagaac
      781 agccaaggaa taccccggtg ctcgctgtgc gaatccccat tttgcgacca caagtcgggc
      841 agtcaatgct ggtcagaacc atctggttcg ggcgcatacc accctcttcc ggatcttttt
      901 cagctttatc taactgctgg gtgaaatccg agaagaagtg atccagtaca gctttccact
      961 ctgcttcgtg atttgccacc aggtcgaggc tgttttccat ctgtgcggtg aagtcgtagt
     1021 tcattaactc gcggaaattt tcttcaaggc gatcggtgac gatttcgcca attttttccg
     1081 catagaaacg acgattttct actcgcacgt agccacgatc ctgaatggtc gaaatgatcg
     1141 acgcataggt agacggacga ccgataccgc gtttttccag ctctttaacc agcgatgctt
     1201 cactgaaacg ggctggcggc ttggtaaagt gctgggctgg tgtaagttca acgagcgtca
     1261 gagcatcgcc tttattaact gctggtaaga tgcgatcttc atcgccttta cgcaacgcag
     1321 gcatcacttt tgtccagcca tcaaaacgca aaatacgacc gcgtgctttc aggcggaaat
     1381 cgcccgcacc aatggtcagc gtcgtggagt catatttcgc tggggtcatc tggcaggcta
     1441 cgaactgacg ccagattaac tggtacagtt tctgcgcatc tgcttccata tccttcagcg
     1501 attccgccat cacattgacg tcagaaggac gaatcgcttc gtgcgcttcc tgtgagtttt
     1561 ctttgctggc gtactgattc ggactttccg gcagatattt cttaccaaaa ttatcgctga
     1621 tataaccacg aaccatattt accgcgtcct gactcaggtt agtggagtcg gtacgcatgt
     1681 aagtgatata gcctgcttca tacaaacgct gcgccatcat catggttttt ttcacgccaa
     1741 atccaagacg ggtgctggca gcttgttgca gcgtagaggt aataaaagga gcgccaggtt
     1801 tactggttgt cggtttgtct tcacgttcca gcacgctgta gcgcgctttt tccagcagac
     1861 ttaccgcagc ctgagtttgt tctttgttga ccggacggaa cggtttgtcg ttctgatgag
     1921 tcacctgtaa cgccaacgct tcaccagatg gcgtggtcgt gctggcatcg acttcccaga
     1981 actcttccgg cacgaacgct ttaatttcac gctcacgctc gaccaccagg cgaaccgcca
     2041 ccgactgcac acgaccggca gacagaccac gagcgatctt tttccatagc agcggcgaaa
     2101 ccatataccc caccacgcgg tccataaagc gacgcgcctg ctgggcatta acacgatcaa
     2161 tattcagctc acccggtttg ttaaatgcct ggcggatcgc gtttttagta atttcgttaa
     2221 acaccactcg gctatagcgc gcatcatcac ccccaatcac ttcccgcagg tgccatgcaa
     2281 tggcttcccc ttcgcggtca aggtcggttg cgagatagat gtggtcggct ttttcagcca
     2341 gttgtttcag ttcagagacg accttctctt taccaggcaa cacttcatag tgcgcctccc
     2401 aattgtgcca cgggtcaacc cccatacggt tgacgagagc gccacgttca tcctttttag
     2461 gctttttagc cgtcttggtg gaggtagagt cggcactctt tttggcagct gagccactgg
     2521 tcggcaaatc gcggatgtga ccgacgctgg atttcaccac gtagtcacta cccagatact
     2581 tgttgatcgt tttggctttt gccggggact caacgatgac aagagcttta cccatattca
     2641 ccttacctaa tttgattctt ccaggaatgc gtcgcacgtt gataaacctt ccactggcaa
     2701 cgagttaccg atatggactc gttctctgcg gatatcaacc ccttgtcgtg actctgttca
     2761 cgaaaattca ggtgactgag ttaacaggtc ttggcctaca ggcgctatag cacgacggaa
     2821 ttttggtcga atgtcaagca aatctattgc cagattgacg aaagcgtcac actgtacctg
     2881 ataaaatccg atacgcaact ttattagcat gcaaaatcca aagttgccag taaggcgctc
     2941 ttgccaccag cgtggtatat ttgtctgcaa gccatgcctg gcgtagacta atgtccttgt
     3001 ttaaggagta aataatgaac aaagagacac aacccatcga ccgcgaaacg ctgttgaaag
     3061 aggccaacaa aatcattcgt gagcatgagg atacgctcgc agggattgaa gccaccggcg
     3121 tcacgcagcg taatggcgtg ctggtattta tcggcgacta ctttttagat gaacaaggat
     3181 tgccaacagc caaaagcacc gcggtgttta atatgttcaa acacctggcc catgtgcttt
     3241 cagaaaaata tcatctggtt gattaaagca aaacgcgagg tcttagcctc gcgtttgtct
     3301 tttacatcaa tggcttttga ccccgctgcc accagcgtag caacaatcga tcggcgctct
     3361 ctgccgcgct gccggtgaat cggtcaatga gtcgtttacg ctgcatatag cgtacattaa
     3421 ccacttcacg gccttccatc aggctaagaa taacttcatc actggtgttg atttcatcaa
     3481 ccaggccttt ctctaccgcc tgttgtccgt accagtgttc acccgttgcc acctgttcaa
     3541 tatccagaga cggacgcata cgcttcacaa aatctttaaa taactgatgc gtttcgttca
     3601 gctcttcgcg gaatttctcc cgcccttctt cggtattttc acccagcaag gtcagcgtgc
     3661 gcttatactg cccggcggtg tgcagttcga tatcaatatc tttgcttttc aggaagcggt
     3721 taaagttggg catttgcgcc accaccccaa tggaacccac aatagcaaac ggtgcggaaa
     3781 caattttgtc cgccacacag gccatcatgt aaccgccgct ggcagcgact ttgtctaccg
     3841 taacagttaa aggaatgttt ttatcacgca gacgctgcag ctgcgaagcc gccaacccgt
     3901 aaccatgcac catgccacca gggctttcca gacggagcac aacctgatcc tgcggtttga
     3961 atgctgcgag taccgccgtt atctcttcac gtagcgagtt cacttcatgg gcgtccatgc
     4021 tgcctttaaa atccagcacc cagacgcgag gtttactgtc agttgccacc tcgcccagtt
     4081 tggctttcgc ttttgctgct ttcgcttctt gcttgtgctt tttcttctgc gctttgtgcc
     4141 actgtttttg ctgatgtgag tccatcagcg ccgcggccag ttcttctttc atctccttat
     4201 actgttcgct gagattgttg acccgtaact cgccacgctg gcgtttatta cgttgagcaa
     4261 cattgacaat aatggcggca atcgccgcaa tcgctagcac aacggtaacg attttcgcca
     4321 aaaacaaacc atattcagac aacaattcca cgagtcccca ccttggttaa acaacgcttt
     4381 ggctcacagt ttacaacagg catgttaagc cgtctcgcag agatgttatt gcagcgaaag
     4441 tatgacaaag tatccatttt gcggtgttat cgccccaggc tgattgaaat gcccttctgt
     4501 ttcaggcata aagccccaaa gtcataaagt acactggcag cgcgacagac aacgtcgttg
     4561 aggagtagcc atgcattacc agccaaaaca agatttactc aatgatcgca ttatcctggt
     4621 gacgggagcc agcgatggga ttggtcgtga agccgcgatg acgtatgcac gctatggtgc
     4681 gacagtgatt ctgttgggcc gtaatgaaga aaaattacgt caggtagcca gccacataaa
     4741 cgaagaaact gggcgtcagc cacagtggtt tattctcgat ttgctgacct gcacgtccga
     4801 aaattgccaa caactggcac agcgcattgt cgttaattat ccgcgtctgg atggtgtttt
     4861 gcataatgcc ggattgctcg gcgatgtttg cccaatgagc gaacaaaatc cgcaggtctg
     4921 gcaggacgtc atgcaggtca acgttaatgc cacctttatg ctcacccagg cactgcttcc
     4981 tttattactc aaatcggacg ccggttcact ggtctttact tcatcaagcg ttggacgtca
     5041 gggacgagcc aactggggtg catatgcagc gtcgaaattt gccaccgaag ggatgatgca
     5101 ggtactggcc gatgaatatc agcagcgcct gcgcgtcaac tgcattaacc caggcggtac
     5161 gcgcaccgca atgcgcgcaa gcgccttccc gaccgaagat ccacagaaac ttaaaacacc
     5221 cgctgatatc atgccgctct acctctggct gatgggcgat gacagccgcc gtaaaaccgg
     5281 catgaccttt gacgcccaac cgggccgtaa accaggaatt tcccaatgag tgatgaacgc
     5341 taccaacagc gtcagcagcg agtgaaagaa aaagtagatg ctcgtgtggc ccaggcccag
     5401 gatgaacgcg gtattatcat cgtctttacc ggcaatggaa aaggcaaaac caccgcggca
     5461 tttggtacgg caacacgcgc agttggtcac ggaaaaaaag tgggcgtcgt gcagtttatt
     5521 aaaggcacct ggcctaatgg cgaacgcaat ctgctggagc cacatggcgt tgagtttcag
     5581 gtgatggcaa cgggctttac ctgggataca caaaaccgcg agtctgatac cgccgcctgc
     5641 cgcgaagtct ggcaacatgc aaagcggatg cttgctgatt cctcactgga tatggttttg
     5701 cttgatgaac tgacgtatat ggtggcgtat gactatttgc cactggaaga agtggtgcag
     5761 gcgttaaatg aacgtccaca tcaacagacg gtgattatca cgggtcgagg ctgtcatcgg
     5821 gatattcttg aactggcgga tacggtaagt gagctgcgcc ccatcaaaca tgcgtttgat
     5881 gccggtgtta aggcgcaaat agggattgat tattaatctg taaaaatgcc ggatatgacg
     5941 tccggcatac tttgattaac cgttattacg cccgccagaa cgacgaccgc cgctcacctg
     6001 actgtgacgt ttcaccgcac gacgaatctg attagccttc atgcgacgac ggtctttttc
     6061 tacagcgact ttagagctgg tttctggcgg aagctccacc agttcgcgca gatagttagt
     6121 ctgggcgagg tccagctccg tccagccacc gcgtggcaga ccttttggca gtgggatatc
     6181 accgtaacga acacggatca ggcggctcac ctgcacacca accgcttccc acagacgacg
     6241 aacttcacgg ttacggcctt cggtcagagt cacgttgtac cactggttga tcccttcgcc
     6301 gccgctgaac ttgatggttt taaaagctgc cggaccatct tccagttgca cgccacgact
     6361 caaatcacgc agtttcgcgt cgtcaacctg accaaataca cgcacggcgt attcacgttc
     6421 aacttcacgg cttggatgca ttaaacggtt tgccagttca ccatcggtgg tgaacagcag
     6481 cagaccacag gtgttaacgt ccagacgacc tacggcaatc cagcgtgcgc cacgcagctt
     6541 tggcagacgg tcaaacaccg tcggacgccc ttccggatcg ttacgggtac acaactcacc
     6601 ttccggtttg taataggcca gaacgcgaca aatttgttca gcggactcac gtaccgaaat
     6661 cagatgacca tcgatacgaa ttttcagccc aggggtaact tcaacgcgat cgccgagttt
     6721 agcaattttg ccatcaacac tcacacgacc ggcttcaata atagattcga tttcacggcg
     6781 ggagccgtgg ccagcgcggg ccagcacttt ctgtaatttt tcgctcatag agcttccttt
     6841 tggtgtcgcc ttcccaggcg tctgatatgc ttttgtgcaa cagtgagtca gaacttacat
     6901 ctgactcact gatagtaaag tctgtttgaa ctcttcaggc cttaagtaaa cgactcgacg
     6961 ttttaactgg cctggctttg aggcgcaaca tactacacta tctttatggt taaagcttgt
     7021 ttcgattaac atcagcttaa ttaccagcca ccaccgcctc cgccacccgc gccgccacca
     7081 gcaccaccac cggaataact tcgccccaac aaactggttt tggcccccag actcaggtgc
     7141 atctgtaatg aaaacaaaac gccactacgg gcaatttccg gggcgctaac agccgctgtg
     7201 agtgatgggg tgatacgtgc tacccattct cttccaaggt ttgctgagac tgcccaacct
     7261 agcaacgcgt aatccagttt ccgtgtttcc cctttccgcc gacgcccact gtgtgttgct
     7321 gcctctttac gcgcaagata gttaacgata gcttcaccgt gggcataacg ttgctgccca
     7381 gtttgtgtaa actttggcag aaaaatatac cccatgccag tgagataacc ggtcaggaaa
     7441 acagcgctaa aataacccgc tggcatatac cagtaaaaga atacatctcc gacattgatg
     7501 tagagagaat agaaggccac gcctgaacaa atcagtggta aacaattctt ccgtcctcaa
     7561 ggatgcggcc atttcgatat gtgtcgccct cttctccagt aaagagatcg atttcgctaa
     7621 atggattacc cgcgatatca gggaacttga gtgaaaatcg ggtcttatag atttcaaacg
     7681 gccagtggtt accagtcacg ttccagatta acagatctga atctccctca cgcaggaaag
     7741 catttttaac ctgataatga atttgataat gatagtcgcc ttgaggcaag aaacgttgtt
     7801 tatcgcccac gacaatactc attaacccga tatgccagtc atgatgccag ggttcaggaa
     7861 taccatcacg ggtgacgcca acaatatgat agttcagcag tgcagcgtcc ccatcaggct
     7921 gcatccagag tcgtggtaaa tcacgataaa atccgtgttt aatctgctga tttcgcgcct
     7981 gaaaatgaat attttctttg atgtcgagca ccccatccgg ttgcagagtg gcttcaacat
     8041 caaagagcga aatcccttct tcataagaag ggatctccgt gttttctgca taacttagag
     8101 atgatacaaa tagccctgct atcaacagca aaatgcagcg aaacttccct gccatagtct
     8161 tccatccgtg gaacgcaatt tgttataaga aaggcttcac atcacctacg ccttcacgca
     8221 ccacgaccgg cgtatcatcg gtaagatcaa taaccgttgt cggtttctgc ccgagatagc
     8281 cgccgtgaat aatcaaatct acctgttttt ccagacggtc tttaatttct tcgggatcgg
     8341 attcggtaaa ttcgctgcct ggcagcatta gcgaagtgga aagcatcggt tcgcccagtg
     8401 cttcaagcaa cgcctgcgca atcgggttag aaggcacacg cataccgatg gttttgcgtt
     8461 tttcctgcaa cagacggcgt ggcacttcct tcgtcccctt caggatgaag gtatagttgc
     8521 ccggcgtgtt gtttttcatt aaacgaaacg caacgttatc aacaaatgaa taggtcgaga
     8581 gttcagaaag atcgcgacac atcagggtaa agttgtgacc gtccggcagc tggcgaatac
     8641 gacaaatacg ctccatcgcg tttttatctt caattttaca gccgagcgca tagccggaat
     8701 cagttggata aacaatcacc ccgcctttac gcacgatctc caccgcctgg ttgatcagac
     8761 gttgctgtgg gttatcagga tgaatataaa aaaactggct cataattccc tctctgtggt
     8821 gttctgcggc tgttcccata gctgccagac gccttcaacg cctgcgggca gccagagttt
     8881 acgacctagt tcgatccacg ggcatggctg atgaaaatca gatccttgtg atgcccataa
     8941 atgatgctga cgcgcaaggg ccgccagctg ggtacgttca ttgggcgatt gctggcactg
     9001 cgcgacttcc atcgcgtcac cgtggtgttc ggcaaaatgc gcaaccaatc ttttcagcca
     9061 tttagcacta agattgtacc gcccaggatg agctagcacc gccttaccgc cagaatgatg
     9121 aatgacatca atagcttgtt ctattgtaca ccactgtggc ggaacgtatc cggttttccc
     9181 gcgcgccaga tattttttaa agacatccgc cattgaactg gctttgccgc accccacaag
     9241 gaaacgcgca aaatgaccgc gcgtcaccgc accgccctgc gccagtcgtc gcgcgccttc
     9301 cagtgcccct ggtatttgcg ctttttcaag tctttcggca atcagttgcg cccgctgatt
     9361 gcgccgttct gtctgctgcg cgaggaactc acacattagc ggatgagtaa tatcaatatt
     9421 cagcccgaca atatgaattt catgattttc ccagaccgtg gaaatttcca cgccgggaat
     9481 aagattcagc gccagtcccg aacgtgaaat ttcctctctt gctggcacga ttgcagctgt
     9541 gttgtcatgg tcggtgatcg ccagggtacc gacgcgcatc tcgactgcac ggtgcaccaa
     9601 tacttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt cgtaaatcac
     9661 tgcgtaattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt tgcgccgaca
     9721 tcataacagt tctggcaaat attctgaaat cagctgttga caattaatca tcgaactagt
     9781 taactagtac gcaagttcac gtaaaaaggg tatcgacaat gaaagcaatt ttcgtactga
     9841 aagcttggtg gcgcacttcc tgaaacgggc agtgtattca ccatgcgtaa agcaatcaga
     9901 tacccagccc gcctaatgag cgggcttttt tttgaacaaa attagagaat aacaatgcaa
     9961 acacaaaaac cgactctcga actgctaacc tgcgaaggcg cttatcgcga caacccgact
    10021 gcgctttttc accagttgtg tgggaatcgt ccggcaacgc tgctgctgga atccgcagat
    10081 atcgacagca aagatgattt aaaaagcctg ctactggtag acagtgcgct gcgcattaca
    10141 gcattaggtg acactgtcac aattcaggca ctttccggca acggcgaagc cctgctggca
    10201 ctactggata acgccctgcc tgcgggtgtg gaaaatgaac aatcaccaaa ctgccgtgtg
    10261 ctgcgcttcc cccctgtcag tccactgctg gatgaagacg cccgcttatg ctccctttcg
    10321 gtttttgacg ctttccgttt attgcagaat ctgttgaatg taccgaagga agaacgagaa
    10381 gcaatgttct tcggcggcct gttctcttat gaccttgtgg cgggatttga agatttaccg
    10441 caactgtcag cggaaaataa ctgccctgat ttctgttttt atctcgctga aacgctgatg
    10501 gtgattgacc atcagaaaaa aaacacccgc attcaggcca gcctgtttgc tccgaatgaa
    10561 gaagaaaaac aacgtctcac tgctcgcctg aacgatcttc gccagcagct gaccgaagcc
    10621 gcgccgccgc tgccggtggt ttccgtgccg catatgcgtt gtgaatgtaa tcagagcgat
    10681 gaagagttcg gtggcgtagt gcgtttgttg caaaaagcga ttcgcgctgg agaaattttc
    10741 caggtggtgc catctcgccg tttctctctg ccctgcccgt caccgctggc ggcctattac
    10801 gtgctgaaaa agagtaatcc cagcccgtac atgtttttta tgcaggataa tgatttcacc
    10861 ctgtttggcg cgtcgccgga aagttcgctc aagtatgacg ccaccagccg ccagattgag
    10921 atctacccga ttgccggaac acgtccacgc ggtcgtcgtg ccgatggctc gctggacaga
    10981 gacctcgaca gccgcatcga actggaaatg cgtaccgatc ataaagagct ttctgaacat
    11041 ctgatgctgg tggatctcgc ccgtaatgat ctggcacgca tttgcacccc cggcagcagc
    11101 tacgtcgccg atcttaccaa agttgaccgt tactcttacg tgatgcacct ggtctcccgc
    11161 gtggtcggtg agctgcgcca cgatctcgac gccctgcacg cttaccgcgc ctgtatgaat
    11221 atggggacgt taagcggtgc gccgaaagta cgcgccatgc agttaattgc cgaggcggaa
    11281 ggtcgtcgcc gcggcagcta cggcggcgcg gtaggttatt tcaccgcgca tggcgatctc
    11341 gacacctgca ttgtgatccg ctcggcgctg gtggaaaacg gtatcgccac cgtgcaagcc
    11401 ggtgctggcg tagtccttga ttctattccg cagtcggaag ccgacgaaac ccgtaataaa
    11461 gcccgcgctg tactgcgcgc tattgccacc gcgcatcatg cacaggagac tttctgatgg
    11521 ctgacattct gctgctcgat aatatcgact cttttacgta caacctggca gatcagttgc
    11581 gcagcaatgg gcataatgtg gtgatttacc gcaaccatat tccggcgcag accttaattg
    11641 aacgcctggc gacgatgagc aatccgatac tgatgctttc tcctggcccc ggtgtgccga
    11701 gcgaagccgg ttgtatgccg gaactcctca cccgcttgcg tggcaagctg cccattattg
    11761 gcatttgcct cggacatcag gcaattgtcg aagcttacgg gggctatgtc ggtcaggcgg
    11821 gcgaaattct tcacggtaaa gcgtcgagca ttgaacatga cggtcaggcg atgtttgccg
    11881 gattaacaaa cccgctgccg gtggcgcgtt atcactcgct ggttggcagt aacattccgg
    11941 ccggtttaac catcaacgcc cattttaatg gcatggtgat ggcagtacgt cacgatgcgg
    12001 atcgcgtttg tggattccag ttccatccgg aatccattct caccacccag ggcgctcgcc
    12061 tgctggaaca aacgctggcc tgggcgcagc agaaactaga gccagccaac acgctgcaac
    12121 cgattctgga aaaactgtat caggcgcaga cgcttagcca acaagaaagc caccagctgt
    12181 tttcagcggt ggtgcgtggc gagctgaagc cggaacaact ggcggcggcg ctggtgagca
    12241 tgaaaattcg cggtgagcac ccgaacgaga tcgccggggc agcaaccgcg ctactggaaa
    12301 acgcagcgcc gttcccgcgc ccagattatc tgtttgctga tatcgtcggt actggcggtg
    12361 acggcagcaa cagtatcaat atttctaccg ccagtgcgtt tgtcgccgcg gcctgtgggc
    12421 tgaaagtggc gaaacacggc aaccgtagcg tctccagtaa atctggttcg tccgatctgc
    12481 tggcggcgtt cggtattaat cttgatatga acgccgataa atcgcgccag gcgctggatg
    12541 agttaggtgt ttgtttcctc tttgcgccga agtatcacac cggattccgc cacgcgatgc
    12601 cggttcgcca gcaactgaaa acccgcaccc tgttcaatgt gctggggcca ttgattaacc
    12661 cggcgcatcc gccgctggcg ttaattggtg tttatagtcc ggaactggtg ctgccgattg
    12721 ccgaaacctt gcgcgtgctg gggtatcaac gcgcggcggt ggtgcacagc ggcgggatgg
    12781 atgaagtttc attacacgcg ccgacaatcg ttgccgagct gcatgacggc gaaattaaga
    12841 gctatcaatt gaccgctgaa gattttggcc tgactcccta ccaccaggag caactggcag
    12901 gcggaacacc ggaagaaaac cgtgacattt taacacgctt gttacaaggt aaaggcgacg
    12961 ccgcccatga agcagccgtc gctgcgaacg tcgccatgtt aatgcgcctg catggccatg
    13021 aagatctgca agccaatgcg caaaccgttc ttgaggtact gcgcagtggt tccgcttacg
    13081 acagagttac cgcgctggcg gcacgagggt aaatgatgca aaccgtttta gcgaaaatcg
    13141 tcgcagacaa ggcgatttgg gtagaaaccc gcaaacagca gcaaccgctg gccagttttc
    13201 agaatgaggt tcagccgagc acgcgacatt tttatgatgc gctacagggt gcacgcacgg
    13261 cgtttattct ggagtgcaag aaagcgtcgc cgtcaaaagg cgtgatccgt gatgatttcg
    13321 atccggcacg cattgcctcc atttataaac attacgcttc ggcaatttcg gtgctgactg
    13381 atgagaaata ttttcagggg agctttgatt tcctccccat cgtcagccaa atcgccccgc
    13441 agccgatttt atgtaaagac ttcattatcg acccttacca gatctatctg gcgcgctatt
    13501 accaggccga tgcctgctta ttaatgcttt cagtactgga tgacgaccaa tatcgccagc
    13561 ttgcagccgt cgctcacagt ctggagatgg gggtgctgac cgaagtcagt aatgaagagg
    13621 aactggagcg cgccattgca ttgggggcaa aggtcgttgg catcaacaac cgcgatctgc
    13681 gtgatttgtc gattgatctc aaccgtaccc gcgagcttgc gccgaaactg gggcacaacg
    13741 tgacggtaat cagcgaatcc ggcatcaata cttacgctca ggtgcgcgag ttaagccact
    13801 tcgctaacgg ttttctgatt ggttcggcgt tgatggccca tgacgatttg cacgccgccg
    13861 tgcgccgggt gttgctgggt gagaataaag tatgtggtct gacgcgtggg caagatgcta
    13921 aagcagctta tgacgcgggc gcgatttacg gtgggttgat tttggttgcg acatcaccgc
    13981 gttgcgtcaa cgttgaacag gcgcaggaag tgatggctgc ggcaccgttg cagtatgttg
    14041 gcgtgttccg caatcacgat attgccgatg tggtggacaa agctaaggtg ttatcgctgg
    14101 cggcagtgca actgcatggt aatgaagatc agctgtatat cgatacgctg cgtgaagctc
    14161 tgccagcaca cgttgccatc tggaaagcat taagcgtcgg tgaaaccctg cccgcccgcg
    14221 agcttcagca cgttgataaa tatgttttag acaacggcca gggtggaagc gggcaacgtt
    14281 tcgactggtc actattaaat ggtcaatcgc ttggcaacgt tctgctggcg gggggcttag
    14341 gcgcagataa ctgcgtggaa gcggcacaaa ccggctgcgc cggacttgat tttaattctg
    14401 ctgtagagtc gcaaccgggc atcaaagacg cacgtctttt ggcctcggtt ttccagacgc
    14461 tgcgcgcata ttaaggaaag gaacaatgac aacattactt aacccctatt ttggtgagtt
    14521 tggcggcatg tacgtgccac aaatcctgat gcctgctctg cgccagctgg aagaagcttt
    14581 tgtcagcgcg caaaaagatc ctgaatttca ggcccagttc aacgacctac tgaaaaacta
    14641 tgccgggcgt ccaaccgcac tgaccaaatg ccagaacatt acggctggca cgaacaccac
    14701 gctgtatctg aagcgcgaag atttgctgca cggcggcgcg cataaaacta accaggtgct
    14761 cggtcaggct ttactggcga agcggatggg taaaaccgaa atcatcgccg aaaccggtgc
    14821 cggtcagcat ggcgtggcgt cggcccttgc cagcgccctg ctcggcctga aatgccgtat
    14881 ttatatgggt gccaaagacg ttgaacgcca gtcgcctaac gtttttcgta tgcgcttaat
    14941 gggtgcggaa gtgatcccgg tgcatagcgg ttccgcgacg ctgaaagatg cctgtaacga
    15001 ggcgctgcgc gactggtccg gtagttacga aaccgcgcac tatatgctgg gcaccgcagc
    15061 tggcccgcat ccttatccga ccattgtgcg tgagtttcag cggatgattg gcgaagaaac
    15121 caaagcgcag attctggaaa gagaaggtcg cctgccggat gccgttatcg cctgtgttgg
    15181 cggcggttcg aatgccatcg gcatgtttgc tgatttcatc aatgaaacca acgtcggcct
    15241 gattggtgtg gagccaggtg gtcacggtat cgaaactggc gagcacggcg caccgctaaa
    15301 acatggtcgc gtgggtatct atttcggtat gaaagcgccg atgatgcaaa ccgaagacgg
    15361 gcagattgaa gaatcttact ccatctccgc cggattggat ttcccgtctg tcggcccaca
    15421 acacgcgtat cttaacagca ctggacgcgc tgattacgtg tctattaccg atgatgaagc
    15481 ccttgaagcc ttcaagacgc tgtgcctaca cgaagggatc atcccggcgc tggaatcctc
    15541 ccacgccctg gcccatgcgc tgaaaatgat gcgcgaaaac ccggaaaaag agcagctact
    15601 ggtggttaac ctttccggtc gcggcgataa agacatcttc accgttcacg atattttgaa
    15661 agcacgaggg gaaatctgat ggaacgctac gaatctctgt ttgcccagtt gaaggagcgc
    15721 aaagaaggcg cattcgttcc tttcgtcacg ctcggggatc cgggcattga gcagtcattg
    15781 aaaattatcg atacgctaat tgaagccggt gctgacgcgc tggagttagg catccccttc
    15841 tccgacccac tggcagatgg cccgacgatt caaaacgcca ctctgcgcgc ctttgcggca
    15901 ggtgtgactc cggcacaatg ttttgaaatg ctggcactga ttcgccagaa acacccgacc
    15961 attcccattg gcctgttgat gtatgccaat ctggtgttta acaaaggcat tgatgagttt
    16021 tatgcccagt gcgagaaagt cggcgtcgat tcggtgctgg ttgccgatgt gccaattgaa
    16081 gagtccgcgc ccttccgcca ggccgcgttg cgccataatg tcgcacctat cttcatctgc
    16141 ccgccaaatg ccgatgacga cctgctgcgc cagatagcct cttacggtcg tggttacacc
    16201 tatttgctgt cacgagcggg cgtgaccggc gcagaaaacc gcgccgcgtt acccctcaat
    16261 catctggttg cgaagctgaa agagtacaac gctgcacctc cattgcaggg gtttggtatt
    16321 tccaccccgg atcaggtaaa agcagcgatt gatgcaggag ctgcgggcgc gatttctggt
    16381 tcagccattg ttaaaatcat cgagcaacat attaatgagc cagagaaaat gctggcggca
    16441 ctgaaagttt ttgtacaacc gatgaaagtg gcgacgcgca gttaatcccc cagccgccag
    16501 ttccgctggc ggcattttaa ctttctttaa cgaagccgga aaaatcctaa attcatttaa
    16561 tatttatctt tttaccgttt cgc
//
LOCUS       DAZPEP010000083        16415 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZPEP010000083 DAZPEP010000000
VERSION     DAZPEP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16415
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.083"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            56..1702
                     /gene="mqo"
                     /locus_tag="ACHUT9_002626"
     CDS             56..1702
                     /gene="mqo"
                     /locus_tag="ACHUT9_002626"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFR1349068.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(2011..2520)
                     /gene="eco"
                     /locus_tag="ACHUT9_002627"
     CDS             complement(2011..2520)
                     /gene="eco"
                     /locus_tag="ACHUT9_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFR1349069.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(2634..2798)
                     /gene="yojO"
                     /locus_tag="ACHUT9_002628"
     CDS             complement(2634..2798)
                     /gene="yojO"
                     /locus_tag="ACHUT9_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFR1349070.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            2907..3401
                     /gene="napF"
                     /locus_tag="ACHUT9_002629"
     CDS             2907..3401
                     /gene="napF"
                     /locus_tag="ACHUT9_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFR1349071.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3391..3654
                     /gene="napD"
                     /locus_tag="ACHUT9_002630"
     CDS             3391..3654
                     /gene="napD"
                     /locus_tag="ACHUT9_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFR1349072.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3651..6137
                     /gene="napA"
                     /locus_tag="ACHUT9_002631"
     CDS             3651..6137
                     /gene="napA"
                     /locus_tag="ACHUT9_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFR1349073.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            6144..6839
                     /gene="napG"
                     /locus_tag="ACHUT9_002632"
     CDS             6144..6839
                     /gene="napG"
                     /locus_tag="ACHUT9_002632"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFR1349074.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6826..7689
                     /gene="napH"
                     /locus_tag="ACHUT9_002633"
     CDS             6826..7689
                     /gene="napH"
                     /locus_tag="ACHUT9_002633"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFR1349075.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7686..8135
                     /gene="napB"
                     /locus_tag="ACHUT9_002634"
     CDS             7686..8135
                     /gene="napB"
                     /locus_tag="ACHUT9_002634"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFR1349076.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            8145..8747
                     /gene="napC"
                     /locus_tag="ACHUT9_002635"
     CDS             8145..8747
                     /gene="napC"
                     /locus_tag="ACHUT9_002635"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFR1349077.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8760..9383
                     /gene="ccmA"
                     /locus_tag="ACHUT9_002636"
     CDS             8760..9383
                     /gene="ccmA"
                     /locus_tag="ACHUT9_002636"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFR1349078.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            9380..10042
                     /gene="ccmB"
                     /locus_tag="ACHUT9_002637"
     CDS             9380..10042
                     /gene="ccmB"
                     /locus_tag="ACHUT9_002637"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFR1349079.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            10084..10821
                     /gene="ccmC"
                     /locus_tag="ACHUT9_002638"
     CDS             10084..10821
                     /gene="ccmC"
                     /locus_tag="ACHUT9_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFR1349080.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10818..11027
                     /gene="ccmD"
                     /locus_tag="ACHUT9_002639"
     CDS             10818..11027
                     /gene="ccmD"
                     /locus_tag="ACHUT9_002639"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFR1349081.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            11024..11503
                     /gene="ccmE"
                     /locus_tag="ACHUT9_002640"
     CDS             11024..11503
                     /gene="ccmE"
                     /locus_tag="ACHUT9_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFR1349082.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11500..13443
                     /gene="ccmF"
                     /locus_tag="ACHUT9_002641"
     CDS             11500..13443
                     /gene="ccmF"
                     /locus_tag="ACHUT9_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFR1349083.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13440..13997
                     /gene="dsbE"
                     /locus_tag="ACHUT9_002642"
     CDS             13440..13997
                     /gene="dsbE"
                     /locus_tag="ACHUT9_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFR1349084.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            13994..15046
                     /gene="ccmH"
                     /locus_tag="ACHUT9_002643"
     CDS             13994..15046
                     /gene="ccmH"
                     /locus_tag="ACHUT9_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFR1349085.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(15081..15728)
                     /gene="narP"
                     /locus_tag="ACHUT9_002644"
     CDS             complement(15081..15728)
                     /gene="narP"
                     /locus_tag="ACHUT9_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFR1349086.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            16048..>16415
                     /locus_tag="ACHUT9_002645"
     CDS             16048..>16415
                     /locus_tag="ACHUT9_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1349087.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         3575 a         4124 c         4774 g         3942 t
ORIGIN      
        1 gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta acaagatgaa
       61 aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt cgatggcggc
      121 aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg gcggcattat
      181 gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga tgaccatggt
      241 ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaacaacg ccggaaccgg
      301 gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca gtatcagtat
      361 tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct gggcgcacca
      421 ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc cgcatatgag
      481 ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg cgttgcaaca
      541 aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca aagagtgggc
      601 accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc gtacggaaat
      661 tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct tgcagaagaa
      721 atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta atgacgataa
      781 cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca ttcgtgccaa
      841 atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat cggggattcc
      901 ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt cggaaaaccc
      961 ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg gcgcaccacc
     1021 gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag tgctgtttgg
     1081 gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg atctgatgag
     1141 ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata atttcgatct
     1201 ggtgaaatac ttggtgagtc aggtgatgtt gagtgaagag gatcgttttg aagcgttgaa
     1261 agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg ggcagcgtgt
     1321 gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta ctgaagtggt
     1381 cagtgaccag caagggacca ttgccgcgct gctgggggca tcgccagggg cgtcaaccgc
     1441 tgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt ccagcccgca
     1501 atggcaggct acgttgaaag cgatcgttcc atcttatgga cgcaagctga acggtgatgt
     1561 agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctgggcctga aatacgacaa
     1621 gccgcaggca gcagatagta cgccgaaacc gcagttgaaa ccgcaacccg ttcaaaaaga
     1681 agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc ggatgtggcg
     1741 taaccgcctt atccgaccta cggggtggag cacttcacag cggtgtaggc ctgataagac
     1801 gcgtcagcgt cgcatcaggc aaccggctcg ggcgttagat gtcggatgcg gcgtaaccgc
     1861 cttatccaac ctacggggtg gagcacttca cagcggtgta ggcctgataa gacgcgccag
     1921 cgtcgcatca ggcaaccggc ttgggcgtta gatgtcggat gcggcgtgac cgccttatcc
     1981 gacctacggg gtgacgcact tcacagcagt ttagcgaact accgcgttgt caattttctc
     2041 ttccgccttc cagacgcggt acttcacatc tacattgtct ggcgtataca ccacgatcgg
     2101 cagcttgctg ttgtaacgca gcattccagc atcgcccaga tacgcggtga caaatttctt
     2161 ctctttcttg ccatccgggc aggccatcat cgtggaaacc ggcgagctaa ctttatcaaa
     2221 gacataataa tcatagcccc agccttccag cgttttgttt tccagcttcc cgccgagacg
     2281 atgcaaattg caatcgactt ccagcgtctg accggttaac agttctactt tcagggtaga
     2341 ttcatcttct tgcggggtta actgaatcac ctgacgcttc atcccttttt cagcttgtgg
     2401 ataaggcgcg attttttcca gtggctggac gctttctgcc gcccaggcgg aagtggtggc
     2461 gaaagcggca aacaatactg caggtagaat ggtcttcatt tttttgcttt ccttcttcat
     2521 ttataggttt tcaccgatga tgtcgctgaa ggttaacata ttcatcagat ttattaattt
     2581 gctttatgaa taacttaaag aacattcaga caattttacc tgctctttta gtgttaaatt
     2641 ctgatgaggg agcaaaccag cagaaatatc tctggtgaat taatcgctat ataaatatgt
     2701 ttataaccat ttgaaatgtg agcaaaagcc cgtttttgcc acgcttcccg ccactctttt
     2761 gatcctgcta caggttttac cccgatcggg gtatgcatct ttgacacatc ctttaatatc
     2821 ttagcggcta taaaaatggc ttattaatta tgcggtttat ttggtcgctc tcaattttca
     2881 gagcgcgtta atgatggaag gtcaatgtga agattgatgc atcccgtcgg ggcatactca
     2941 ctggtcgctg gcgcaaagcc agtaacggta tccgtccgcc ctggtccggt gatgaatctc
     3001 attttctgac ccattgcacc cgttgcgacg cctgtattaa tgcttgtgaa aacaacattc
     3061 tgcaacgcgg cgcgggcggc tatccgagcg ttaatttcaa aaataacgag tgcagcttct
     3121 gttacgcctg cgcgcaggct tgccccgaat cgctattttc tccgcgccac accagggcct
     3181 gggatttgca gttcaccatt ggggacgcgt gcctggcgta tcagtcagtt gaatgccgcc
     3241 gctgtcagga tagctgtgag ccaatggcaa ttatctttcg cccgacgctc tccgggatct
     3301 accagccgca acttaatagc caactttgca acggatgtgg agcatgtgca gccagctgtc
     3361 cggtatcagc cataactgcg gagtatctcc atgcacacta actggcaagt ttgcagcctg
     3421 gtcgtgcagg ccaaaagcga acgaatttca gacatcagca cccaactgaa cgcctttccc
     3481 ggctgtgaag ttgctgtcag cgacgcgccg agcggtcagt tgattgtggt ggtggaagca
     3541 gaagacagcg aaacgctgat ccaaaccatt gagtcagtac gcaacgtaga gggcgtgctg
     3601 gcggtgtcgc tggtttatca ccagcaggaa gagcaaggtg aggaaacacc atgaaactca
     3661 gtcgtcgtag ctttatgaaa gctaacgccg ttgcggccgc tgcggcggct gccggtctca
     3721 gcgtgccggg cgttgcccgc gccgttgttg gtcagcagga agccattaaa tgggataaag
     3781 cgccgtgccg tttctgcggt actggttgcg gcgttctggt cggaacgcag caggggcgtg
     3841 tggtggcctg tcagggcgac ccggacgcac cggttaaccg tggcctgaac tgcattaagg
     3901 gctatttcct gcccaagatc atgtacggta aagaccgttt gacgcagccg ctgctgcgta
     3961 tgaaaaacgg taaatatgac aaagaaggcg aatttacccc aatcacctgg gatcaggcct
     4021 tcgatgtgat ggaagagaaa ttcaaaaccg ccctgaaaga aaaagggccg gaatcgatcg
     4081 gtatgttcgg ttctggtcag tggactatct gggaaggtta tgccgcgtcc aagctgttta
     4141 aagcgggctt ccgttcgaac aacatcgacc cgaacgcgcg tcactgtatg gcgtcggcag
     4201 tagttggctt tatgcgtacc tttggtatgg atgagccgat gggctgctat gacgacatcg
     4261 agcaggctga cgcgtttgtg ctgtggggcg caaacatggc ggagatgcac ccgatcctct
     4321 ggtcgcgcat cactaaccgt cgtctctcta atcagaacgt caccgtggcg gtgctttcta
     4381 cctaccagca tcgtagcttc gagctggcgg ataacggcat catctttacg ccacagtctg
     4441 acctggtgat cctgaactac atcgccaact atatcattca aaacaatgcg ataaatcagg
     4501 acttcttcag caaacacgtt aacctgcgca aaggggcgac ggacatcggc tacggtttac
     4561 gtccgaccca tccgctggaa aaagcagcga agaatccagg ttctgacgcc tccgaaccga
     4621 tgagctttga agattacaaa gccttcgttg ccgagtatac gctggaaaaa accgctgaaa
     4681 tgaccggcgt gccgaaagac cagttagaac aactggcgca gctgtatgcc gatccgaaca
     4741 agaaagtcat ctcctactgg acgatgggct tcaaccagca tactcgtggc gtgtgggcta
     4801 acaacctggt ctacaacctg cacctgctga ccggcaaaat ttcccagccg ggttgcggtc
     4861 cgttctccct gaccgggcaa ccttctgcgt gtggtactgc gcgtgaagtg ggcacctttg
     4921 ctcaccgtct gcctgcggac atggtggtga ccaacgagaa acaccgtgat atctgcgaga
     4981 agaagtggaa tatcccgagc ggcaccattc cggcgaaaat cggtctgcac gcggtggcac
     5041 aagaccgtgc gctgaaagac ggcaaactga atgtttactg gaccatgtgt accaacaaca
     5101 tgcaggccgg gccgaacatc aatgaagagc gtatgccggg ctggcgcgat ccgcgcaact
     5161 tcatcatcgt ctccgatccg tatccgacag tcagtgcgct ggccgccgac ttgatcctgc
     5221 cgaccgcaat gtgggtagag aaagagggtg cttacggtaa cgccgaacgc cgtactcagt
     5281 tctggcgtca gcaggtacag gcaccgggcg aagcgaaatc ggatctctgg cagttagtcc
     5341 agttctcccg ccgcttcaaa actgaagaag tgtggccgga agagctgcta gcgaagaaac
     5401 cggaactgcg tggcaaaacg ctgtacgaag tgctttatgc cacaccggaa gtgagcaaat
     5461 tcccggtatc cgaactggcg gaagatcagc tgaacgatga atcccgcgag ctgggcttct
     5521 atctgcaaaa agggctgttc gaagagtacg catggtttgg tcgcggtcac ggtcacgatc
     5581 tcgcgccgtt cgatgactac cacaaagcgc gcggtctgcg ctggccggtg gtgaacggta
     5641 aagaaacgca gtggcgttac agcgaaggta acgacccgta cgtgaaagcg ggcgaaggct
     5701 ataagttcta cggtaaaccg gatggtaaag cggtgatctt cgcgctgccg ttcgaaccgg
     5761 cggcggaagc accggatgaa gagtacgacc tgtggctctc taccggacgc gttctggagc
     5821 actggcacac tggcagtatg actcgccgtg tgccggaact gcaccgcgcc ttcccggaag
     5881 cggtcttgtt tattcacccg ctggatgcga aagcgcgcga tctgcgccgt ggcgacaaag
     5941 tgaaagtggt ttctcgccgt ggcgaagtga tctcgattgt tgaaacgcgc ggtcgtaacc
     6001 gtccgccaca gggcctggtg tacatgccgt tcttcgacgc cgcacagctg gttaacaaac
     6061 taacgctgga cgcgaccgat ccgctctcga aagagacgga cttcaagaag tgcgcagtca
     6121 aactggagaa ggtgtaagcc attatgtccc ggtcagcgaa acctcaaaat ggtcgccgcc
     6181 gctttctgcg cgatgttgtt cgcacagcag gcgggctggc tgccgtgggt gtggcgctgg
     6241 ggttacaaca gcaaaccgca cgcgcatctg gcgtgcggtt gcgcccgccc ggagccataa
     6301 acgagaacgc ctttgccagt gcctgtgtgc gttgcggtca gtgtgttcag gcttgccctt
     6361 acgacacctt aaaactggcg acgctggctt ctggcctgtc ggcgggcacg ccatattttg
     6421 tcgcacggga tattccttgc gaaatgtgtg aggacattcc gtgcgccaaa gtgtgcccga
     6481 gtggtgcgct ggatcgtgag atcgaatcga tcgacgacgc gcggatgggg ctggcggtgc
     6541 tggtggacca ggaaaactgt ctcaactttc aggggctgcg ctgcgatgtt tgttatcgcg
     6601 aatgcccgaa aattgatgag gccatcaccc tggagctgga gcgcaacacg cgtaccggta
     6661 agcacgcccg ctttctgccg acggttcaca gcgacgcctg taccggctgc ggtaagtgcg
     6721 aaaaagtgtg cgtgctggaa caaccggcaa tcaaggtgtt accgctgtca ctggcgaaag
     6781 gggagttagg tcaccattac cgcttcggct ggctggaggg gaacaatggc aaatcgtaaa
     6841 cgtgacgccg ggcgcgaggc gctggtgaaa aaaggctggt ggcgcagtca ccgttggctg
     6901 gtgttacgtc gcctttgtca gttcttcgtg ctggggatgt ttttgagcgg tccgtggttt
     6961 ggtgtgtgga tcttacacgg caactacagc tgtagcctgt tattcgacac cgttccgctg
     7021 accgatccgc tgatgacgct gcaaagtctt gccagtggtc atctgcctgc cacggtggcg
     7081 ttgaccggtg cggtgataat cactgtgctc tacgccctgg caggaaagcg attattttgc
     7141 agctgggtct gcccgctgaa cccgattacc gacttagcga actggttacg caggcgcttt
     7201 gacctcaatc agtctgcgac gatccctcgc cacattcgct acgtgctgtt ggtggtcatt
     7261 ctgctgggat cagccttaac cggcacgctc atctgggaat ggatcaaccc cgtttccctg
     7321 atggggcgta gcctggtaat gggcttcggc agcggcgcgc tgcttattct cgcgctgttt
     7381 ttatttgatc tactggtcgt tgaacacggc tggtgcgggc acatttgtcc ggttggcgcg
     7441 ctgtatggcg tgctgggtag caaaggtgtg attaccgttg cggccagcga tcggcagaaa
     7501 tgtaaccgct gtatggattg ttttcatgtt tgcccggaac cgcatgtgct acgtgccccg
     7561 gtgctggatg aacaaagccc ggtgcaggtc accagccgcg attgcatgac gtgcggtcgc
     7621 tgcgtggatg tctgttctga ggatgtattt acaataacta cacgatggag ttcgggagcg
     7681 aaatcatgaa aagccatgac ctgaagaaag cgctgtgtca atggacggcg atgctggccc
     7741 tggtggtaag cggcgcggtt tgggcggcta atggcgtcga ttttagccag tcgccggaag
     7801 tatccgggac gcaggaaggg gccattcgga tgccaaaaga gcaggatcgg atgccgctga
     7861 actatgtgaa tcagccgcca atgatcccgc atagcgttga aggttatcag gtaacgacca
     7921 ataccaaccg ctgcttgcag tgccacggtg tcgaaagcta tcgcaccact ggcgcgccgc
     7981 gtatcagtcc tactcacttt atggacagcg acggcaaagt gggcgcggaa gtggctccgc
     8041 gtcgttattt ctgtctgcaa tgtcacgtac cgcaggccga taccgcgcca atcgtgggga
     8101 atacctttac cccatcaaaa ggttacggga aataagaggt tattatggga aattctgacc
     8161 gtaagcctgg tctgattaag cgcctgtgga aatggtggcg tacccccagc cgtctggcgc
     8221 tgggaacgct gctgttgatc ggttttgtcg gcggcatcgt cttctggggc ggctttaaca
     8281 ccggaatgga aaaagccaat accgaagagt tctgcattag ctgccacgaa atgcgtaaca
     8341 cggtgtatca ggaatacatg gattccgtgc actacaacaa ccgtagcggc gtccgtgcga
     8401 cctgtccgga ttgtcacgtt ccgcacgagt ttgtgccgaa gatgatacgc aagctcaaag
     8461 caagtaaaga gctgtatggt aaaatttttg gcgttattga cacgccgcag aaatttgaag
     8521 ctcatcgtct gacaatggca cagaatgagt ggcggcgcat gaaggacaat aactcgcagg
     8581 agtgccgtaa ctgtcacaac ttcgagtata tggatacaac cgcccagaaa tcggttgccg
     8641 cgaagatgca tgaccaggcg gtgaaagatg ggcaaacctg tattgattgc cataaaggga
     8701 tagcgcacaa gctgcccgat atgcgtgaag tcgagccagg tttttaacag ggttattgcg
     8761 tgggtatgct tgaagccaga gagttacttt gtgagcggga tgaacgaacc ttatttagtg
     8821 gcttgtcatt tacgctgaac gcaggagagt gggtacaaat caccggtagc aacggcgcgg
     8881 ggaagacaac gcttctccgt ttgctgacgg ggttgtctcg ccctgacgca ggcgaggttc
     8941 tctggcaagg gcagcccttg catcaggtac gcgacagcta ccatcaaaac ctgttatgga
     9001 taggccatca gccggggatc aaaacccggc tgacggcgtt agaaaatctg cacttttatc
     9061 atcgcgatgg cgataccgca caatgtctgg aagccctggc gcaggccggg cttgccggat
     9121 tcgacgatat tccggtaaat caactttctg ccgggcaaca acgccgcgtc gctttagcgc
     9181 gtctgtggct gacccgtgcc acgttatgga tcctcgacga gccttttacc gcgattgacg
     9241 ttaacggcgt cgatcgtctg acccagcgta tggcgcagca tacggagcag ggggggattg
     9301 tgattctgac tacccaccag ccgctcaacg ttgctgaaag taaaattcgc cgcatttcac
     9361 tgacgcaaac gagggccgca tgatgttctg gcgcattttc cgtcttgagc tgcgtgtagc
     9421 gtttcgccat agcgccgaaa tcgccaaccc gctgtggttc ttcctgattg taattaccct
     9481 ttttccgctc agtatcggtc cggagccgca actgctggcg cgtattgcac cgggcattat
     9541 ctgggttgct gcgctgcttt catccttgct ggcgctggaa cgactgttcc gtgacgattt
     9601 gcaggacggc agtcttgaac aattgatgtt gttgccgtta cccttgcccg ccgttgtgct
     9661 ggcgaaggtg atggcgcact ggatggtaac cggtctgccg ttactcatcc tttcgccact
     9721 ggtagcaatg ctactgggaa tggatgttta tggctggcaa gtgatggcgc tgacgctgct
     9781 gctgggaacg cctacgcttg gctttctcgg tgcaccgggt gtggcgttga cagtgggact
     9841 taagcgcggt ggtgtgctgc tcagcatact ggtgttaccg ctgactatcc cattactcat
     9901 ctttgccacc gccgcgatgg acgcggcttc catgcatttg cccgttgacg ggtatctggc
     9961 aattttaggc gcgttgctgg caggcaccgc gacattaagt ccttttgcga cggcggcagc
    10021 gttacgaatc agcattcaat aacgctggat tactgatttt tgttcgagtc tggtatcgaa
    10081 actatgtgga aaacactgca tcaactggcg atcccaccac ggctgtatca aatctgtggc
    10141 tggtttatac cgtggctggc aattgccagt gtggtcgtgc ttaccgtcgg ctggatctgg
    10201 ggattcggct ttgctccggc tgattatcag cagggaaata gctaccgcat tatctacctg
    10261 catgtgcctg cggcgatctg gtcgatgggc atttatgcat caatggcagt ggcagcgttt
    10321 attggccttg tctggcagat gaaaatggcc aacctggcgg tggcggcgat ggcccccatt
    10381 ggtgccgtgt ttacctttat tgccctggtt accggctctg catggggaaa accgatgtgg
    10441 ggcacctggt gggtatggga tgcacgtctg acttctgaac tggtgctgct gtttttgtat
    10501 gtgggtgtga ttgccctgtg gcacgccttc gacgaccgcc gtctggcggg ccgtgcggca
    10561 ggtatcctgg tgctgattgg cgtggtgaat ctgccgatta ttcattactc cgtggagtgg
    10621 tggaacaccc tgcatcaggg atcaacgcgg atgcagcaaa gtatcgatcc ggcgatgcgt
    10681 tcgccgctgc gctggtcgat ttttggcttc ctgctcctgt ctgccacgct gacgctgatg
    10741 cggatgcgta atttgatttt gctgatggaa aaacgccgtc cgtgggtgag tgaactgata
    10801 ctgaaaagag gccgtaaatg acccctgcat ttgcttcctg gaatgaattt ttcgcaatgg
    10861 gcggttacgc cttttttgtc tggctggcgg tggtgatgac cgttattccg ctggtggttt
    10921 tggtcgtgca ctcggtgatg caacatcgcg caattctgcg tggtgtggcg caacagcggg
    10981 cgcgtgaggc gcgtttacgt gctgcgcaac agcaggaggc tgcatgaata ttcgccgtaa
    11041 aaaccgcttg tggattgcct gtgccgtgtt ggcagggctg gcgctgacta tcggtctggt
    11101 gctatatgcg ctgcgctcga atatcgatct cttttatacg ccgggggaaa ttctctacgg
    11161 caagcgtgaa actcagcaaa tgccggaagt cggtcagcgt ctgcgcgttg gcgggatggt
    11221 gatgctgggt agtgtgcagc gcgatcccaa ttcgctgaaa gtgaccttca ctatttacga
    11281 tgctgaaggc tcagtggatg tctcttacga aggcattttg ccggatctgt tccgtgaagg
    11341 gcagggcgtt gtggtgcagg gcgagctgga aaaaggcaat catatcctcg cgaaagaagt
    11401 gctggcgaaa cacgacgaaa actacacgcc gccagaagtt gagaaagcga tggaagctaa
    11461 ccaccgtcgc ccggcgagtg tttataagga cccagcatca tgatgccgga aattggaaac
    11521 gggctgctgt gtctggcgtt agggattgcg ctgctgctgt ccgtgtatcc gctatggggc
    11581 gtggcgcgcg gagatgcgcg catgatcgcg tcttcccgct tgtttgcctg gctgctgttt
    11641 atgtctgtgg ctggcgcatt tctggtgctg gtcaatgctt tcgtggtcaa cgactttacc
    11701 gtcacctatg ttgccagcta ctccaatacc cagcttccgg tgtggtatcg cgtggcggct
    11761 acctggggcg cgcatgaagg ctcgctactg ctgtgggtgc tgctgatgag cggctggacc
    11821 tttgcggtgg cgatttttag tcagcgtatt ccgctggata ttgtggcccg cgtactggcg
    11881 ataatgggga tggtcagcgt cggctttttg ctgttcattc tctttacctc taacccattc
    11941 tcacgcacgc tgccgaactt cccgattgaa gggcgcgatc ttaacccgct gttacaggac
    12001 ccggggctga tcttccatcc gcctctgctt tatatggggt acgtgggttt ctcggtggcg
    12061 tttgcttttg ccattgcttc tttgctgagc gggcgtctgg acagcactta tgcgcgtttt
    12121 actcgcccgt ggacgctggc ggcgtggatt ttcctgacgc tcggcattgt gctcggttcc
    12181 gcatgggcct attacgaact cggctggggg ggctggtggt tctgggatcc ggtagaaaac
    12241 gcctcgttta tgccgtggct ggtggggact gcgctgatgc actcactggc ggtcactgaa
    12301 caacgcgcca gcttcaaagc gtggacatta ctgctggcaa tcagtgcctt ctcgttgtgt
    12361 ctgttgggga ctttcctcgt gcgttccggc gtgctggtat cggtacacgc gtttgcgtct
    12421 gatccggcgc gcggtatgtt tatcctcgcc tttatggtgc tggtgattgg aggttcgctg
    12481 ctgctgtttg ccgcgcgtgg acacaaagtt cgctcacgcg taaacaatgc gctgtggtcg
    12541 cgggaatctc tgctgttagc gaacaacgtt ttgctggtcg ccgcgatgct ggtggtgttg
    12601 ctggggacgc tgctgccgct ggtgcacaag caactgggac tgggcagtat ttcgattggc
    12661 gaaccgttct tcaacaccat gtttacctgg ctgatggtgc cgtttgcgct actgctgggt
    12721 gtcggtcctc tggtgcgctg ggggcgcgat cgcccacgta aaatccgcaa tttattgatt
    12781 atcgccttca tctcaacgct ggtgctgtct ctgctgttgc cgtggctgtt tgagagcaaa
    12841 gttgtggcga tgacggtgct cggcctggca atggcctgct ggattgcggt gctggcaatt
    12901 gcggaagctg cgctgcgtat ttcacgcggc acgaaaacca ccttcagtta ttgggggatg
    12961 gtggctgctc acctggggct ggcagtgaca attgttggca ttgcttttag ccagaactat
    13021 agcgttgagc gtgatgtgcg catgaagtcc ggcgatagcg tcgatattca tgaatatcgc
    13081 ttcaccttcc gtgatgtcaa agaggtgact ggcccgaact ggcgtggcgg tgtggcgact
    13141 atcggcgtaa cgcgagaggg taaaccggaa acagtgctgt atgcggaaaa acgttattac
    13201 aacactgccg ggtcgatgat gaccgaagcg gcgattgacg gtggcatcac gcgtgacctg
    13261 tacgccgcgc tcggtgaaga gctggaaaac ggcgcgtggg ctgtgcgtct ttactacaaa
    13321 ccatttgttc gctggatttg ggcgggcggg ctgatgatgg cgttgggcgg actgctgtgt
    13381 ctgtttgatc ctcgctatcg taagcgcgtg agtccgcaaa aaactgcgcc ggaggctgta
    13441 tgaagcgcaa agtattgtta attccgttga ttatcttcct ggcgattgcc gcggcgctgc
    13501 tgtggcagct ggcgcgtaat gccgaagggg atgatccgac caatctggaa tcggcgctca
    13561 ttggcaagcc agtgccgaag tttcgcctcg aatcactgga caatccgggg cagttttatc
    13621 aggcggatgt gctgactcag ggcaaaccag tgctgcttaa cgtctgggcg acctggtgtc
    13681 cgacctgccg tgcggaacat caatatctga atcagctttc tgcgcagggc atccgcgtag
    13741 tcggcatgaa ctataaagac gatcgccaga aggcaatcag ctggctgaaa gagctgggca
    13801 atccttacgc gctaagcctg tttgatggcg atggcatgtt agggctggat ctcggtgtct
    13861 atggcgcgcc agaaacgttc cttattgacg gcaacggcat cattcgctat cgccatgcgg
    13921 gcgatctcaa tcctcgcgtc tgggaagaag agatcaagcc gctgtgggag aaatacagta
    13981 aggaggccgc acaatgaggt ttttattggg catgctgatg ctgatgatct ccggctcagc
    14041 gctggcgacc atcgacgtgt tgcagtttaa agatgaagca caggaacaac agttccgtca
    14101 gctcactgaa gaactgcgct gcccgaaatg ccagaacaac agcattgccg attccaactc
    14161 gatgattgcc accgacctgc gccagaaagt gtatgaactg atgcaggaag gtaaaagtaa
    14221 aaaagagatt gtcgattata tggtggcgcg ttacggcaac ttcgtcactt acgatccgcc
    14281 gttaacgccg ctgaccgtgc tgctgtgggt gctgccagta gtagctattg gcattggcgg
    14341 ttgggtcata tacgcccgct cgcggcgtcg ggtacgcgtg gtgccggaag cgtttcctga
    14401 acaaagcgtg ccggaaggta agcgtgccgg atatgtcgct tatttgccgg gcattgtggt
    14461 ggcgttaatt gtggctggcg tcagctacta ccagaccgga aattatcaac aggtgaaaat
    14521 ctggcagcag gccacggcac aggctccggc gttgctggac agggcgctgg atccgaaagc
    14581 tgatccgctc aacgaagaag agatgtcgcg ccttgcgctg gggatgcgta ctcaactgca
    14641 aaaaaatccg ggagatatag acggctggat tatgttgggc cgcgttggca tggcgctggg
    14701 taacgccagt atcgccaccg atgcatacgc tactgcgtat cgcctcgatc cgaaaaacag
    14761 tgatgccgca ctgggttatg ctgaagcgtt gacacgttca tctgatccca acgacaaccg
    14821 cctcggcggc gaactgctac gtcagctggt gagaacggac catagcaata tccgtgtgct
    14881 aagcatgtat gcgtttaatg cctttgagca gcagcgattt ggcgaagccg ttgccgcgtg
    14941 ggagatgatg ttgaaactct tacctgccaa cgatactcgc cgtgcggtga ttgaacgtag
    15001 tatcgcgcag gcgatgcaac atttgtcgcc gcaggagagt aaataaaaag aggcccgatg
    15061 gtgatgccat cgggctattt ttattgtgcc ccgcgttgtt gcaggaacag aatggtggcc
    15121 gccacgcgtg agcggacatt gagtttacgc agcagatttc gaatatgaac ttttactgtc
    15181 tgctcggaaa tattcaacac agaggcaatc tgtttatttg acagcccctg tgccagctcg
    15241 tgcagaacat ccagctcgcg ctccgtcagc acgctgaagg gatcttcttc cgcgccaaac
    15301 atttcacgtt cacgtaagta ctgattgacg cgttcgctaa agactttgct gcctttcgct
    15361 ccggcacgaa tcgcttccag caacacttcc gggtcgctgt ctttcagcag ataaccgtct
    15421 gcgcctgcgt ctatcagcgc aaagacatcg ctggaggcat cggatacggt caggataata
    15481 atttgcgcgg taacgccatc cctgcgcaag gcattgaggg tatccaggcc actcatacct
    15541 ttcatattga gatccagcaa gatcacgtcg atatccagtc tattcgccag atcgatcgcg
    15601 ctcgcgccgt cgcccgcttc ggcgaccact tcaaagccag gatcaagctc cagtaactga
    15661 cgaacaccgc gtcgcataag tggatgatca tccacaatca tcacctgaaa aggcgttgct
    15721 tcaggcatag tagtctcctg aggttttatt agaatcatta tgtttgagcg tagcgttggt
    15781 aacgctacca ggcgcgtgaa tgttacccca ttttatgtat ggacatgtag tgaaaactac
    15841 aaccttgccg ttcgctttgg catcattttc attataaaga cggaattttg catcagcttc
    15901 tcatcaagaa aaacaactaa aaaatcaatc acaatacttg tgcatttctt taaacagttt
    15961 ttaaaaactg actccgggta tggagctatg ggtattttct gtacccaatg cttttaacag
    16021 caattaattt cataggatga aagctcaatg catcaatctg gttctgtttc tctttgtcgt
    16081 tccgcaatat ctgttctggt ggctacagcg ttatattcac ccatagcatt ggcatcaaca
    16141 gttgagtatg gtgagacagt tgatggtgtt ggcctggaaa aagatatcca gctggtttat
    16201 gggaccgcca ataatacgaa aatcaatcct ggcggagaac agcatataaa agaatttggt
    16261 gtaagtagta atactgaaat taacggcggg tatcagtaca ttgaaatgaa tggcactgca
    16321 gaatactcag tattaaatga tggttatcaa attgttcaaa tgggtggcgc ggtaaaccag
    16381 actacgctca ataatggagt gctacaggtt tatgg
//
LOCUS       DAZPEP010000084        16400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZPEP010000084 DAZPEP010000000
VERSION     DAZPEP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.084"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            78..2411
                     /gene="bisC"
                     /locus_tag="ACHUT9_002646"
     CDS             78..2411
                     /gene="bisC"
                     /locus_tag="ACHUT9_002646"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFR1349088.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2380..2820)
                     /locus_tag="ACHUT9_002647"
     CDS             complement(2380..2820)
                     /locus_tag="ACHUT9_002647"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1349089.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHUT9_002648"
     CDS             complement(2817..3380)
                     /gene="tag"
                     /locus_tag="ACHUT9_002648"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFR1349090.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3538..4236
                     /locus_tag="ACHUT9_002649"
     CDS             3538..4236
                     /locus_tag="ACHUT9_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFR1349091.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4465..5673
                     /locus_tag="ACHUT9_002650"
     CDS             4465..5673
                     /locus_tag="ACHUT9_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1349092.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5997..7688
                     /gene="eptB"
                     /locus_tag="ACHUT9_002651"
     CDS             5997..7688
                     /gene="eptB"
                     /locus_tag="ACHUT9_002651"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFR1349093.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7780..7856
                     /locus_tag="ACHUT9_002652"
     tRNA            7780..7856
                     /locus_tag="ACHUT9_002652"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7814..7816,aa:Pro,seq:cgg)
     gene            7891..8024
                     /locus_tag="ACHUT9_002653"
     ncRNA           7891..8024
                     /ncRNA_class="other"
                     /locus_tag="ACHUT9_002653"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8769..10376
                     /gene="dppA"
                     /locus_tag="ACHUT9_002654"
     CDS             8769..10376
                     /gene="dppA"
                     /locus_tag="ACHUT9_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFR1349094.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10684..11703
                     /gene="dppB"
                     /locus_tag="ACHUT9_002655"
     CDS             10684..11703
                     /gene="dppB"
                     /locus_tag="ACHUT9_002655"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFR1349095.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11713..12615
                     /gene="dppC"
                     /locus_tag="ACHUT9_002656"
     CDS             11713..12615
                     /gene="dppC"
                     /locus_tag="ACHUT9_002656"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFR1349096.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12626..13609
                     /gene="dppD"
                     /locus_tag="ACHUT9_002657"
     CDS             12626..13609
                     /gene="dppD"
                     /locus_tag="ACHUT9_002657"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFR1349097.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13606..14610
                     /gene="dppF"
                     /locus_tag="ACHUT9_002658"
     CDS             13606..14610
                     /gene="dppF"
                     /locus_tag="ACHUT9_002658"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFR1349098.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14640..15911)
                     /locus_tag="ACHUT9_002659"
     CDS             complement(14640..15911)
                     /locus_tag="ACHUT9_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFR1349099.1"
                     /translation="MQHNTLSKHNQKLPFTCYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         3901 a         4093 c         4408 g         3998 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     1201 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     1261 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     1321 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     1381 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     1441 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     1501 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     1561 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     1621 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     1681 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     1741 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     1801 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     1861 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     1921 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     1981 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     2041 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     2101 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     2161 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     2221 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     2281 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     2341 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     2401 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     2461 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     2521 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     2581 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     2641 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     2701 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     2761 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     2821 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     2881 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgct
     2941 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     3001 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     3061 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     3121 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     3181 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     3241 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     3301 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     3361 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     3421 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     3481 tccttcatac aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     3541 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     3601 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     3661 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctaatta caacgatatt
     3721 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     3781 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     3841 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     3901 aacgagtttg gcgacagtac taccacgcag accgatccgt tatggcatgc cagcgtgagt
     3961 accttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     4021 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     4081 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     4141 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     4201 tatctgtata cgatgggggt tagcgccaga ttttaacgta atagtcacaa ttgaaaccat
     4261 taaataacaa taattgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     4321 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     4381 tgccgttttt ccccaggcat aaagtgcact tcgttatggt tgtcggcaga gatttttcct
     4441 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     4501 acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac ctggagcctg
     4561 tttaatggcg cgctttccgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     4621 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt acaggaacgt
     4681 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     4741 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtctg
     4801 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     4861 aaaggtctga tctccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     4921 tttatcgaca cgcagctgct cgaaacggtg ggtctggaaa aaacatttgt gatttgggga
     4981 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     5041 gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga gtcgatgcgt
     5101 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     5161 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     5221 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     5281 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     5341 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     5401 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     5461 ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     5521 agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac cttctacgtg
     5581 attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca gccagagcag
     5641 aaaatgttgc gtgaggcgca tggctccctt taatgctcag ccgcgagcta ctgggcttca
     5701 gcttccgtgt gcatcaaacg catcaacgaa atgtataaac aatgactcgt aaaatttggg
     5761 ccagttaact ggcccttttt actgcttttt gtaacgctaa agtctctttt caaacttgca
     5821 tttttgtaaa tttgtgcttc atgcacactc tttccccaca ctttttcgct ttgctgtggt
     5881 ctacttattc gcgcgtgtag attttactta tctgactacc tccgcacttt tcccctgccg
     5941 ggcctgaaaa gccgttaagc agggtgttat cacctgtttg tccagggttt gtttgcatga
     6001 gatacatcaa atcgattaca cagcagaagc tgagcttttt gcttgcaatc tatattggcc
     6061 tttttatgaa tggcgcggtt ttttatcgcc gcttcggcag ctatgcgcac gattttaccg
     6121 tctggaaagg cgtttctgct gttgttgaac tggccgccac cgtactggtg accttctttt
     6181 tactacgtct tctatcgctg tttggccgcc gcagctggcg tattctggcg tcgctggtgg
     6241 tgctcttttc cgcaggtgcc agctattaca tgaccttcct taatgtggtc attggttatg
     6301 gcatcatcgc ttccgtcatg accaccgata tcgacctgtc aaaagaagtt gttggtctga
     6361 actttattct ctggttaatc gccgttagtg cattgcctct tatccttatc tggaataacc
     6421 gctgtcgcta caccttgctc cgacaactgc gaaccccagg gcagcgtatt cgcagcctgg
     6481 cggtcgtcgt actggcgggt attatggttt gggcaccgat tcgtttgctg gatatccagc
     6541 agaagaaagt ggagagggcg accggcgttg atttgccgag ttatggcggt gtcgtagcga
     6601 actcttatct gccatcaaac tggctttctg cgttggggct gtatgcctgg gcgcgggtcg
     6661 atgaatcttc cgataataat tcattgctta atccggcgaa gaagtttaca taccaggctc
     6721 cacaaaacgt tgatgatact tatgtcgtgt ttatcatcgg tgaaaccacg cgttgggacc
     6781 atatgggtat tttcggctat gagcgtaata ccacgccgaa actggcccag gagaaaaatc
     6841 tggcggcgtt ccgtggttac tcctgtgata ccgcaaccaa actctcactg cgttgcatgt
     6901 ttgtacgtca ggggggcgcg gaagataatc cgcagcgcac attaaaagaa cagaacattt
     6961 tcgcggttct gaagcagtta ggattcagtt ctgacctcta cgctatgcag agcgaaatgt
     7021 ggttctacag caacacgatg gcggacaaca ttgcttatcg tgagcagatt ggtgcggagc
     7081 cacgtaatcg tggcaagccg gtagatgata tgttgctggt agacgaaatg cagcaatcgc
     7141 tagggcgcaa cccggatggt aagcatctga tcattctgca taccaaaggt tcgcatttta
     7201 actacaccca gcgttatccg cgtagcttcg cgcagtggaa gccggaatgt attggtgttg
     7261 atagcggctg taccaaagcg cagatgatca actcctatga caactcggtg acctatgtgg
     7321 atcactttat ctccagcgtg attgatcagg ttcgcgataa gaaagcgatt gtgttctacg
     7381 cagctgacca cggtgagtca attaatgaac gcgagcacct gcacggcacg ccgcgtgaac
     7441 tggcaccgcc ggagcagttc cgcgtaccga tgatggtctg gatgtcagat aaatatctgg
     7501 aaaatccggc caatgcgcag gcgtttgcgc agctgaaaaa agaagccgac atgaaagtgc
     7561 cacgccgtca cgtagagctg tacgacacca tcatgggttg tcttggctat acttcaccgg
     7621 atggtggaat taacgaaaac aacaactggt gtcacatccc gcagacaaaa gaggcagcgg
     7681 ctaactaaga ggcctgctga ctttctcgcc gatcaaaagg cattttgcta ttaagggatt
     7741 gacgagggcg tatctgcgca gtaagatgcg ccccgcattc ggtgattggc gcagcttggt
     7801 agcgcacttc gttcgggacg aaggggtcgg aggttcgaat cctctatcac cgaccaaatt
     7861 cgaaaagcct gctcagcgag caggcttttt tgcatttgaa gcccatgagg atgagaacct
     7921 ccgggggcgg ggaggttcga ctcgagcgaa gcgagagaac gttgcgccag caacggcccg
     7981 cagggcgagc cacgaagtgg cgagtaatcc tctatcaccg accaaattcg aaaagcctgc
     8041 tcaacgagca ggcttttttg catttgtagc tcatgaggat gagaacctcc gggggcaggg
     8101 aggttcgact cgacgtaggc ctgataagac gcgccagcgt cgcatcaggc aacggcaccg
     8161 catttattgt cggatgcggc gcgaccgcct tattcgacca acacaccaca cattttcctg
     8221 ccaactttgc gccccatatc tcactatcca ttaacggatt tgtgacagaa accattgtct
     8281 ttttttatat ttgcttaaat ctttttttgc gttacttatg gctggcgctg gcattttccg
     8341 ctgtgcgttt tagcgttcat ggtattaatc gctcagatat ccaccttttc ctcagatttt
     8401 tttcattaaa tgcataaaac ttaatcacca aatgttgcta aatattaagc tgattgttct
     8461 ggttatgttg gagtagcaca aatttctctg ctgcaaatag ctgtttgaag tttttttaat
     8521 ctttgtttgt ggattctcac cgttggtgta aatcccggtt ggctgtattg acgttttcac
     8581 attctgttga cagattgtag gtcacgaggg gcattttatg gaggatccgc actgttacac
     8641 tgatgttaat tagtacggca tccccacctc ataacgttga cccgaccggg caaaaaacaa
     8701 aaaaggtcag gcagcgacaa cccactgcaa agggttaaaa caacaaacat cacaattgga
     8761 gcagaataat gcgtatttcc ttgaaaaagt cagggatgct gaagcttggt ctcagcctgg
     8821 tggctatgac cgtcgcagca agtgttcagg ctaaaactct ggtttattgc tcagaaggat
     8881 ctccggaagg gtttaacccg cagctgttta cctccggcac cacctatgac gcctcttccg
     8941 taccgcttta taaccgcctg gttgaattta aaatcggcac cactgaagtg atcccgggcc
     9001 tcgctgaaaa gtgggaagtc agcgaagacg gtaaaaccta taccttccat ctgcgtaaag
     9061 gtgtgaagtg gcacgacaat aaagaattca aaccgacgcg tgaactgaac gccgatgacg
     9121 tggtgttctc gttcgaccgt cagaaaaacg cgcaaaaccc gtaccataaa gtctctggcg
     9181 gcagctacga atacttcgaa ggcatgggct tgccggagct gatcagtgaa gtgaaaaagg
     9241 tggacgacaa cacagttcag ttcgtactga ctcgcccgga agcgccgttc ctcgctgatc
     9301 tggcaatgga cttcgcctct attctgtcaa aagaatatgc tgatgcgatg atgaaagccg
     9361 gtacaccgga aaaactggac ctcaacccaa tcggaaccgg tccgttccag ttacagcagt
     9421 accaaaaaga ttcccgtatc cgctacaaag cgtttgatgg ctactggggc accaaaccgc
     9481 agatcgatac gctggttttc tctattaccc ctgatgcttc cgtgcgttac gcgaaattgc
     9541 agaagaatga atgccaggtg atgccgtacc cgaacccggc agacatcgct cgcatgaagc
     9601 aggataaatc catcaacctg atggaaatgc cggggctgaa cgtcggctac ctctcctata
     9661 acgtgcagaa aaaaccgctg gatgacgtga aagttcgcca ggcactgacc tacgcggtga
     9721 acaaagacgc gatcatcaaa gcggtttatc agggcgcggg tgtatcagcg aaaaacctga
     9781 tcccgccaac catgtggggc tataacgacg acgttcagga ttacacctac gatcctgaaa
     9841 aagcgaaagc cttgctgaaa gaagcgggtc tggaaaaagg tttctccatc gacctgtggg
     9901 cgatgccggt acaacgtccg tataacccga acgctcgccg catggcggag atgattcagg
     9961 cagactgggc gaaagtgggc gtgcaggcca aaatcgtcac ctacgaatgg ggtgagtacc
    10021 tcaagcgtgc gaaagatggc gaacatcaga cggtaatgat gggctggact ggcgataacg
    10081 gggacccgga taacttcttc gccaccctgt tcagctgcgc cgcctctgaa caaggttcta
    10141 actactcaaa atggtgctac aaaccgtttg aagatctgat tcaaccggcg cgtgctaccg
    10201 acgaccataa caaacgcgtt gaactgtaca agcaagcgca ggtggtgatg cacgatcaga
    10261 ctccggcact gatcatcgct cactccaccg tgtttgaacc ggtacgcaaa gaagtcaaag
    10321 gctatgtggt tgatccatta ggcaaacatc acttcgaaaa cgtctctatc gaataattaa
    10381 aagccataca agactgatgg caaaggcaaa actgcctgat gtgctccgct tatcaggcct
    10441 acgaaaattc tgcaatgtat tgaatttgca cgattttgta ggccggataa ggcgttaacg
    10501 ccgcatccgg cataaacaaa gcgcactttg tcaacaatct gtatacccgg tggcgctgtg
    10561 cctctgcgtg cagcgccatc cggcagcaat acttcattcc ctgtccgata agggcgggaa
    10621 ttgatttgtg agcaatacag acacgcagtt ccaggctgcg ggtcactata gagaatccgg
    10681 gttatgttgc agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt
    10741 acccttctca catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggca
    10801 ggcgaacgcg ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat
    10861 aaaccgatgt ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc
    10921 atttcaatga aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg
    10981 ctggaacttg gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg
    11041 ctggctgcgg ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc
    11101 ggctactcga tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac
    11161 tggaacctga cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat
    11221 ccgttaaccg gttttatgct gatcgacacc gccatctggg gtgaagatgg caactttatc
    11281 gacgccgttg cccatatgat cttaccggct attgtgctgg gtactatccc gctggcggtt
    11341 atcgttcgta tgacgcgctc ttcgatgctg gaagtgctgg gtgaagatta catccgcacc
    11401 gcgcgcgcca aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg
    11461 atgctgccgg tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt
    11521 ctgaccgaaa ccatcttctc gtggcccggt ctgggacgct ggttgattga cgcactgcaa
    11581 cgccgcgact atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc
    11641 gtcaacctgc tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag
    11701 taaggggaca tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg
    11761 accccgttac aggagttctg gcactatttt aaacgcaaca aaggcgcggt tgtcgggctg
    11821 gtttacatcg tcatcgtgct gtttatcacg atctttgcca actggattgc gccttataac
    11881 ccggcggatc agttccgcga tgcgctgctc gccccgccag cctggcagga aggcggcagc
    11941 atggcgcact tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg gctgatgtac
    12001 ggcgcgcgct tgtcgctgct ggttggctgt ctggtggtgg tgttatcgct gattatgggc
    12061 gttattctcg gcctgatcgc cggttacttc ggcggcctgg tcgataacat catcatgcgc
    12121 gtggtcgata tcatgctggc gctgccaagt ctgctgttgg cactggtgct ggtggcgatt
    12181 ttcggcccgt cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat
    12241 gtgcgcttaa cccgcgccgc cgtgctggtg gaggtgaatc gcgattacgt taccgcgtct
    12301 cgcgtggcag gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctg
    12361 gcgccgctga ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggccgcc
    12421 cttggcttcc tcggcatggg agcgcagccg ccaacaccag agtggggcac catgctctcc
    12481 gacgtgttgc agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc
    12541 ctgctgacgg tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc
    12601 aaactgaagc agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca
    12661 tttcggcgac gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca
    12721 gggcgaagtg gtcgggattg tgggtgagtc tggctccggt aagtcggtca gttcactggc
    12781 gattatgggg ctgattgatt atccgggccg cgtaatggcg gaaaagctgg tgtttaacgg
    12841 tcaggatttg cagcgtatat cagaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc
    12901 gatgatcttc caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat
    12961 catggaagcg attaaggtgc atcagggcgg taataagagc actcgccgtc agcgggcgat
    13021 tgacctgctg aatcaggtcg gtattcccga tccggcatcg cgtctggatg tttacccgca
    13081 tcagctttcc ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcggcc
    13141 aaaactgctg attgccgatg agccgaccac tgcgctggac gtgaccattc aggcgcaaat
    13201 catcgaactg ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca
    13261 tgatctggcg ctggtggcgg aagctgcgca taaaatcatt gtgatgtatg caggccaggt
    13321 ggtggaaacg ggcgatgcgc acgccatctt ccatgcgccg cgtcacccgt atactcaggc
    13381 attgctgcgt gcgctgccag aatttgctca ggacaaagaa cgtctggcgt cgttgccagg
    13441 tgtcgttccc ggcaagtacg accgcccgaa cggctgcctg cttaacccgc gctgccccta
    13501 tgccactgac agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgcca
    13561 gtccaaatgc cattacccac ttgatgatgc cgggaggccg acactatgag tacgcaagag
    13621 gccaccctgc aacaaccgct gttgcaggct atcgacctga aaaaacatta tccggtgaag
    13681 aaaggcatgt tcgcgccgga acgtctggtt aaagcgctgg atggcgtttc gtttaacctt
    13741 gaacgtggca aaacgctggc agtagtgggc gaatctggct gcggtaaatc gaccctcggt
    13801 cggttgctga cgatgattga aacgcccacc ggtggcgagc tgtattacca ggggcaggat
    13861 ctgcttaagc acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc
    13921 cagaaccctt acggttcgct gaatccgcgt aaaaaagtcg ggcaaattct tgaagagccg
    13981 ctgctgatca acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg
    14041 gcgaaagtcg gcctgaaaac cgagcactat gaccgctatc cgcatatgtt ctccggcggt
    14101 cagcgtcagc gtatcgccat cgcccgtggt ctgatgctcg acccggatgt ggtgattgcc
    14161 gatgaaccgg tttccgcgct ggatgtttca gtgcgcgcgc aggtgctgaa tctgatgatg
    14221 gatttgcagc aggagttggg gctgtcttat gtctttatct cccacgacct gtcggtggtg
    14281 gagcacattg ctgatgaagt gatggtgatg tacctgggcc gctgcgtgga gaagggaacg
    14341 aaagaccaaa tcttcaataa cccgcgccat ccgtacactc aggcgctact ttccgcgacg
    14401 ccgcgcctga acccggacga tcgccgcgag cgcatcaagc tcagcggtga actaccaagc
    14461 ccactgaatc caccgccggg ttgcgccttc aacgcccgct gtcgtcggcg cttcggcccc
    14521 tgcacccagt tgcagccgca gctaaaagac tacggcggtc aactggtagc ttgttttgct
    14581 gttgatcagg atgaaaatcc gcagcgttaa ctcacaaaac cggggccatg cgctccggtt
    14641 tactcaatca gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc
    14701 caggctttaa atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga
    14761 atgaggcacg acacaatacc atataacggg cttcccaact gaaagaacac cagcaccgat
    14821 acacgaaacg aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc
    14881 agcacgcgtg agttaatttt cttggtatca ataattcggc ttaacagatt gagaataatg
    14941 cctttaatgg cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag
    15001 atattaagca ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagc
    15061 gccagtgctg agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag
    15121 gtaaacgaaa aggcaaaaaa caggatcacc gcgatgagcg tgatataact aattcggtgg
    15181 gtacgcagcg cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata
    15241 ttcattgggt taagtacctg aataaatact gcagaaaaga agcaaaatgg aatggtaagc
    15301 aagacatcgc ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag
    15361 tgcgggatca tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc
    15421 ccggaaatct taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa
    15481 atagcgactt tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc
    15541 aggtacgagg cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc
    15601 atcaaaaagt agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata
    15661 atatcggtgt agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc
    15721 actacccagg tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc
    15781 aagccaattt gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat
    15841 aaaacccagc cgaagtcgta gcatgtaaac ggcaatttct gattgtgttt cgatagtgtg
    15901 ttgtgctgca taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag
    15961 tttctcttgc gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag
    16021 attcactggg gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt
    16081 ctggagtcag cccggatatg aactgcaact attgcaaata aagaaattaa gctggaaggc
    16141 atcgcgcaat gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg
    16201 caagccgaaa gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg
    16261 gttgctggag agagaaaacc cccgcacgtt gcaggtatgt acctgacaac ataacggggg
    16321 ctaatcttga acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc
    16381 ggctatatga cgctcgcaga
//
LOCUS       DAZPEP010000085        16273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZPEP010000085 DAZPEP010000000
VERSION     DAZPEP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.085"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(164..979)
                     /gene="yahL"
                     /locus_tag="ACHUT9_002660"
     CDS             complement(164..979)
                     /gene="yahL"
                     /locus_tag="ACHUT9_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFR1349100.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDC
                     KCILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="ACHUT9_002661"
     CDS             complement(1222..2271)
                     /gene="yahK"
                     /locus_tag="ACHUT9_002661"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFR1349101.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(2358..2432)
                     /locus_tag="ACHUT9_002662"
                     /pseudo
     CDS             complement(2358..2432)
                     /locus_tag="ACHUT9_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2648..4030)
                     /locus_tag="ACHUT9_002663"
     CDS             complement(2648..4030)
                     /locus_tag="ACHUT9_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFR1349102.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(4040..4990)
                     /locus_tag="ACHUT9_002664"
     CDS             complement(4040..4990)
                     /locus_tag="ACHUT9_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFR1349103.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(5133..6551)
                     /locus_tag="ACHUT9_002665"
     CDS             complement(5133..6551)
                     /locus_tag="ACHUT9_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1349104.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(6551..8098)
                     /gene="fdrA"
                     /locus_tag="ACHUT9_002666"
     CDS             complement(6551..8098)
                     /gene="fdrA"
                     /locus_tag="ACHUT9_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFR1349105.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(8088..8951)
                     /locus_tag="ACHUT9_002667"
     CDS             complement(8088..8951)
                     /locus_tag="ACHUT9_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1349106.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(8991..9596)
                     /locus_tag="ACHUT9_002668"
     CDS             complement(8991..9596)
                     /locus_tag="ACHUT9_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFR1349107.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDYTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            9854..10351
                     /locus_tag="ACHUT9_002669"
     CDS             9854..10351
                     /locus_tag="ACHUT9_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFR1349108.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALTAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            10443..11375
                     /locus_tag="ACHUT9_002670"
     CDS             10443..11375
                     /locus_tag="ACHUT9_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1349109.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(11417..12505)
                     /locus_tag="ACHUT9_002671"
     CDS             complement(11417..12505)
                     /locus_tag="ACHUT9_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1349110.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(12647..>16273)
                     /gene="ehaA"
                     /locus_tag="ACHUT9_002672"
     CDS             complement(12647..>16273)
                     /gene="ehaA"
                     /locus_tag="ACHUT9_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFR1349111.1"
                     /translation="TGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIHVKG
                     TSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNASEKISLGYYDQ
                     NSDTTLAVSEGGKISAPTISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKTSEK
                     KITLNHTDKNATISADIVSDSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGALGIT
                     QNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYIDVAS
                     GAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFDEFA
                     YTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGGTVD
                     FGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVRSVSRDIDSALSLTEVDDGNS
                     AIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTSGVN
                     NDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQTVS
                     LSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNGSAD
                     SLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSANVLV
                     SHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAGNVL
                     VVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLNGIE
                     LIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDPMPN
                     PEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGNTYY
                     TDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSDSWH
                     VGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQYSW
                     FDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTWMGV
                     KADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLHNSK
                     DFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAMVGI
                     KWQF"
BASE COUNT         3775 a         4392 c         3854 g         4252 t
ORIGIN      
        1 actgctggtg cttttttaag cgaagtgtcg ccgatcacaa caaaatatcg gcgaaaaggc
       61 ggtggaatgc catttggcat acggatatac agatgaaatg attacctgct ttgcgattca
      121 ctctctggtg gaataacaac ttgcaggtgg aaccctttct gaactaaaga gtgtagcgga
      181 ttttaacgtg cttatttaca ggataatatc tgtcacctaa ttcctgcact ctattagata
      241 tccattcatt taatcgtgca tcatctctgt atgtttttac gtccagaaca gagagcaata
      301 acatcaccag tgattcagga aaaacagggt agatattttt taagtgtaat aataatccat
      361 gcgcaagcgc ctgcttaatg ggcgcgtctg tctccggaat agacgacatt tcgcgaagcc
      421 atttttccac atgcatacac tctttccctt tagggaaaag tctcagcaaa ggcatatggt
      481 gttcaagaag aatacatttg caatctgttc gcactgaatt gataaactgc actaatttct
      541 taagcccggt aaagcgtcca gcctgcacat ttgctttaat atcaagaaac gcatcatcct
      601 ggtcatatac ccaaactgtc cctttttgat tttcaagtat gcgaaaaaca aacttaaccg
      661 ggtgatcagg gtaatccatc cagtcataaa atcctgggaa atcattaaac tgcacattta
      721 tggtgagtgc gttatcgtct gaccactcac agtctcgtac ggcagccatg agatgaatga
      781 gtttgtcgtc atcgactaaa tcatcacgac aatcataagg ataatcataa tattgtttaa
      841 tcaaaacctt gaatatatcg acacaggaaa ccagggagct tttgatatcc tctggtaact
      901 gattgttttt aaccgtatta agtatgcttt gttgcgtata tggcatcaaa ttattgtgcg
      961 gagcttttaa tgatatcatc gcgatctctc ttaaataaaa ataaacgtcg cagataataa
     1021 agcgctgatt aaaccaggca ctatcagaaa tcgctcataa ctaacgtgag aagtattgtg
     1081 tactggaggg agttaattta acaatttgaa tttacgttat aggttttact gctactttga
     1141 tattatttcg ctctccttaa ctaaaaagtt aagtggagag cgaagttaat atgttaaacc
     1201 acagcgtatt tattaatttt ttcaatctgt tagtgtgcga ttatcgataa caaaacgata
     1261 tttcacatca ccacgcagca ttcgctcata ggcttcatta atttgatcgg cccgaatcat
     1321 ctctatatca gccacgatgc catgttcggc gcaaaaatcg agcatctcct gagtttctgg
     1381 aatgccgcca atcatagaat cggctatcgc acggcgtttc atgatcaggt tgaaaacttc
     1441 cggcgattta tgcggtgtcg caggcgcacc aaccagcgtc atggtgccat cacgcttcag
     1501 caaggtggta aaatcgtcga gattatgtgg cgcagctact gtattcaaaa tgaaatcgaa
     1561 actcttcaga tgagccgcca tctcatcggc attgcgtgag ttaacaactt catcggcccc
     1621 cagggctttt tccgcttcgc gttttgcctc agaagtggta aatgccacca catgtgcccc
     1681 catcgcgtgg gccagcttaa tccccatatg tcccagaccg ccgatgccga ccacgcccac
     1741 ttttttaccc ggcccggcct gccagtgacg tagcggcgag tacgtggtga tccctgcaca
     1801 caacaaagga gccaccgccg ccagctgctc ttgcgggtga cgaatacgca gaacatatcg
     1861 ctcatgaacg acgatctgtt gtgagtagcc gcccagagta tggcccggtt cgtccggcgt
     1921 cggcgagtta taggtgccga tcatgtgatc acagtagttt tccagcccgt cctcacactc
     1981 ttcgcaatgt ttacaactgt cgacaatgca gccgacaccg accagatcgc ccggcgcatg
     2041 tttttctacc tgatcaccaa cggctaccac acgccccaca atttcatgac ccggtacgca
     2101 ggggtaaacc gtccccgccc actcggaacg gacctggtgg agatcggaat ggcagacgcc
     2161 acagtaagcg atttcgattt tgacatcatt cggtcccggt tcacgccgtg tgatatccat
     2221 cggttcaagt ggttgtttag cggaatatgc accaacagct ttgatcttca ttgtgtttac
     2281 tcctgattag ctatgtgtat ttggcgacgc ctgatatgtc atcgcggcaa aacgcgtcca
     2341 ttgaatatag ccaatattta aaccacccga tccagcccca acatgccgcg aacctgaatg
     2401 aaacagagca gcaacgttcc ccataaggcg agcgcctata agtgtgatat ttgctatccg
     2461 taaaagacta aaatcggcaa aactgatgat cttttcacgt agacaaatag ttatctacac
     2521 aacgtcaata agggcatatt cctcaaagat aatgtccatt tctgccgaca ctatcatatt
     2581 gaggtcgccc cactccctgg ggcttaaatc tcttacaatt caccgttgca ggcgtttcaa
     2641 atgcgcatca acctgccaca ctcccccaca ccagttgccc tttatggaac gttgcggttc
     2701 tcggcgagat acgcgccacc gcctcggcgg aacaggaggc gtccaccagc acaaagctgg
     2761 catcatcctg cgcttttggc catacacgct cgcctttttc attcagcggc aatacatcgc
     2821 cagtggctag aaacagcgaa cgggagaggt tctgttcgtt aggacgaata tagagctgcg
     2881 cgtagagatt ggctttttcc agcatgtcgc ccagaccata aggcgaccag tggtcgataa
     2941 cgctgtcagt gccagtcatc acttttacgc ctttgtcgtg caactgtttg agcggcatat
     3001 gcagcgtgcc aatcggcacc gtcgaggcga tagaaatttg ctgcgccgcc atccgattcg
     3061 ccagcgcatc gacctgttgt tcgttgagcg tagccagcgc aaaggcgtgg ctgatggtca
     3121 gcttaccttt cagttgcggc gttttctcta ccgtttcaac catataattg atggctgcca
     3181 cgcccgacgg agtggtttcg tgcaggtgaa tatcgacacc tttgtcgtag tccagcgcaa
     3241 tctgaaacat ggtgtcgagg gatttttcca tcgcgccatc gacactggtc gggtccagcc
     3301 cgccgacgta atgcgcaccc gcctgcatcg cttcgcgcat taagggttcc gatttcgaca
     3361 gcagcaaacc gtgctgcggg aaggcgacga tttcacactc gaagcccgcc tgacgccgcg
     3421 ccagcaccgc ctgcaaattt tgcagatttt ttaggccgga aaccggttcg atattgcagt
     3481 ggctgcgggc aatggtggtg cctttcgact gcaatagatc gatcagtttt tccgcccgct
     3541 cctgcgtata cggttgtaac tccggcagca ttttctgctt gagtttgatc atgtcctgga
     3601 tagtggtgcc tgccggacga ttgagcgagc gccacggccc gccgtagaag gttttgtcga
     3661 gatgaatatg catgtcgcga gtggtgggta gcatcaactt accgccagcg tcatagtgcg
     3721 gcagcgtggc gtccggatgc tgcttgttct cgcgcagggc gacaattttg ccgtcctgaa
     3781 tctccacggt ctggcgcgcc gtgcgggtct gcaccgccac accgttttcg tagtcaaaac
     3841 cggtttccag cagcacgtta tcgagatagt aatgcgggtc agtgattttc atggtgttgt
     3901 tcgcttcgca gtttagggtg ccagctaccg ccgcatgggc gagcggcaca taggtaccaa
     3961 acagcgcggc ggcggtgacc atcttaccgc tctggctcag aaattcacgg cggctattgc
     4021 tttctttcat cctacgtcct tacgttttaa taatatgggt gcccgtttca ccgcgcagcg
     4081 ccgcaggcag gcattccggc gtggtgataa tcacttcttt gccgccctgc tccaggaacg
     4141 tcaggctggc gatgattttt ggcaacatgc tgccgggaga aaaatgccct tcctgcatat
     4201 agcgagtcat ggtggcaata tccacccgat ccagcgcctg ctgctgcggt ttaccaaagt
     4261 ggatacagac tttttcgacg tcggtggtga tcaccagaat gtcggcgtga atttcacggg
     4321 ccagcagcgc ggtagagaga tctttgtcaa taaccgcgtc cacgctttgg taatcccccg
     4381 cttcagtacg cactaccgga attccaccgc cgcccgcgcc aattacgaca aaaccttgtt
     4441 ggatcagcgc cttaatggcg ggtgcttcga caatacgttt cggttccggc gaggcgacca
     4501 cgcgacgata gccccgtccg gcatcttcaa caaaacacca gtcagggttt gccttttgta
     4561 gtttgtcacg ctggctttca ctgaagaatg caccaatggg cttggtggga tgggcaaaac
     4621 ctggatcgtt tttatccact tccacctgag ttaccacggt gacggctttt ttctcgccgt
     4681 gacgcgccag tcggttattc agcgcctgtt ggatcagata gccgatgccg ccctgcgtat
     4741 ctgccacaca gttcgccagc ggcgttaagg gcagcccttc gcgctcgtgg gcaatctccg
     4801 cacggcgtaa atccagcccg acctgcggcc cgttaccgtg ggtcagcaca atgtcgtaat
     4861 cggaagccag catttccagc accgtatcgg ccacggcttt caccgcctcc gcctgatgct
     4921 caatcgactg gctggcgtta tctttgataa tgctgttgcc accaatggca acgaccacaa
     4981 gctctttcat gttgttatgt ccgctacaga gggtgatgag gttatgacca acgccagact
     5041 gcctgatgcg ctacgcttat caggcctaca attctcgggc catccgtagg ccggataagg
     5101 cgttcacgcc gcatccggca taaacatgta agtcagacgc cgtaacgttc gcaccagccg
     5161 agaatggctt tctcgaagca agcgaatggt ggatgcacaa tgcccgcgcc aatcatgccg
     5221 acgcccgcat ctttatgggc gatggcggtg ttgatcaccg gcagaatgcc gctgctaccc
     5281 acgcgggtga tgtcgattgc cgacggcacg cccataaaac cgagcagcgg aatggtgacg
     5341 ttggggtttt caccgagagt gatttcacgc atctgacggg agaaatcgat agcctcttcc
     5401 accgtgccgc ccaccagcgc gacgattgca ggcgcggtcg ccatagcaaa tccaccaata
     5461 ccgtaggttt cggtgatggc gctgtcgccg atatccagcc ccgaatcttc cggcttataa
     5521 ccggcaaaca tcgggccgat cacctgctgc gccgggccgg taaaccattg ccccggcagg
     5581 ccgctgaccc gcaggccgaa ctcgacgccg ttacgcgcca tggtggtgac cacggtgctg
     5641 tattcgatgc cgtgcgccgc atccatcgcc gctttacaca tcgccatcca cgtcgggccg
     5701 gagaagtagt cgctgctggc gacaaactcg aacacttcgc gctgttgctc gacggaatag
     5761 cctgcctgaa tgatccccgg cgtcagtgcc tgaatcagca gcgtcgtccc ggcgttattg
     5821 cggttatggc actcatcgcc catatgcagc gcctgcgcca gcattaagcg cagatcgatc
     5881 tcaccgataa ttttcatcgc gtcgcgcagc attggcccct gcacatcgcg catccagttg
     5941 aggcggtcga tcacgctctg atcgttggca cccatacgca aaatcttcgc catctgctcg
     6001 ctcatattgg tgtaagcgat gttgccgtag gttttgtttt tcacgatgtg cataaacatc
     6061 gaggccgagg taacgcccgc catcgatccc acacagtcgt gttcgtggca cggcgagaag
     6121 gtgatctccc cggaggccgc cagttcagcc gcctcgtcga gatctttcgc cagtccttcg
     6181 aacaccagcg ctccggtgac cgcgcctttc atcgcgccgc acattttttc ccaggtgatc
     6241 ggcggcccgg cgtgaagaat ggttttcggc gtcatgcccg gcaccacgtt aatcgcctga
     6301 tcaaaaccaa tcagcaccgg atgcgactgg ataatgcgct ccagcgcctg ctggttagcg
     6361 gcggcgattt tgtctgccag cggcgaatcg gcaatgttat ccagcgcctg caccacctgc
     6421 atattgccct gccccggcgg cgtccagtcg agttgagtca cttctacatg ctgctttttc
     6481 aggtcatcgc taaacatggc gatgccgacg ttaataacgt tcaatggttg gctaaacagt
     6541 gactggctca tcaggcttcc tcccctttgc agataaattc acgcgccagc aatccggtat
     6601 tggtgctgct gctcgccaga atcactccgg catcaagcag catctggctt tgttgttcta
     6661 acgatggcgt atcaagatcg gtacccagca cataggcgag aatgatcaac tcgcgtcctt
     6721 cggcagcggc gatcgctttc gcttctttga tcgcctcgat ggtggagccg accggatctt
     6781 catgcgatcc aaatccgagc acaaaatcca tcacgatcac cgccacttct ggatcgcgcg
     6841 cctcttcgat caagcgactg atgcggttgg tggggtcaat cattggatgc ggcttgccgt
     6901 tcgtgaagtc gtcatcgcca aagtcgagga aggtgtgttt gatgctgcgg ttgatatctt
     6961 gcaggcggaa ttccggatcg ggctgaatgt tgctgtagac atcgccatgt ttttccatca
     7021 ccgcgaacat ggtttcgtcg cacagcgtgc cgccgcagaa caggccacga atgtatttct
     7081 gctgcggttg cagacgcgca cgcacatcag caatcaacgg ctggttaagc gtatgcaggt
     7141 cgagattttc ctgtttcacg ccggagagca tcaccgcttt tagcgccgcc tctttgctgc
     7201 cgcgggcaaa ctgtagcccc tgctcatcca ccggcgtttc aacgcgaccg aggaagcaga
     7261 ccaccaccgg cttgcggcag gcacgtgcgc gttccagcac tttgcgggcc accgcaggcg
     7321 cgggaggttt tgaaatcagt gcgataattt cagtttgcgg atcgttttcc agcatcccga
     7381 tggcgtcgag catcatcagg ccgccgattt tctcgctcag gtcgcgcccg ccggtgccaa
     7441 tcagttgtga aacaccgcca ccaaattcat gaatgcggac gctcagctcc tggctgccgg
     7501 tgccggatgc gccaacaata ccgatattgc cgcgacgcac ggcgttacca aaacagagcg
     7561 ccgcgccgtt aataatcgcc gtgccgcagt ccggccccat catcagcagc cctttttcgt
     7621 gggccaactg cttgagcgcc agttcatctt caactgagac gttatcggaa aacagcatca
     7681 cgttgagatc gttttgcagc gcctgacgcg cttcgcgagc ggcaaacaga ccgttgaccg
     7741 aaatcaccgc caggttactt tccgggatat gctttttggc gctggcaata gtggcgtaac
     7801 gcgcctcgtg cgagccgctt tgcgctttgg tgttgaacag ttcttcaatc tccaccagca
     7861 actgctcgtt gtccgcaccc gatttaccat tgatgacaat catcaggtcg ccgtttttcg
     7921 cctgttccag cgtcggcgtc agcagtccta aatttttcag cacacctttg ttcatttcgg
     7981 tcgccatcgc cacaaatgcc tgctcgacgc cgtcgagttt atttgcacgc gtggagatag
     8041 acatcagcga gacagaatca aaataggtat tcggtttaat gactatttta actgacataa
     8101 ttccctccgt aggtttggct cagcgcacaa ccatcggcca tgccatacag catgtcgcag
     8161 ccggaactgg agccaatatg tttaattttt tctattgcct gagttgaatt cccagggacg
     8221 ccataaataa tgttgtgaat aaagcgatga atattttccc ggcagcgttg ttgtaatgcg
     8281 gcttccagcg ttatggcact taataacgtg gtgttatttc tgccgcgtag cagaccgcga
     8341 taaaattcct ctttgtattt ttctgccgga tgcccgggaa taaataaaat aagcgccaga
     8401 cctgttaaat aatcatcggc tgatggcgta aggccaatcc ctaacccaat catactggcg
     8461 accgctgcgg cgatattttg cttatcgttt actgtctgaa taactgcccg tcgtttaata
     8521 tgtaattctt tatttaacgc ctgataaaac ggattatcgc cgcgataatt aaacagggtt
     8581 tcattttccc ccaggtgctg atgaataatg tcgtgccact gtagccaggc gataaggtga
     8641 aaattcacag cggtcatttg caaggttggg gacagccaac gccgacaacg actcatctct
     8701 atatgaagat gttgaccaac cgtaatacct tgatcgtcaa actgaacctt atcaccatgc
     8761 cggaacagat catcaaagtg agtgagtgcc aaccgacaac tgtttggcgc attgtcgtac
     8821 tcttcacaaa gcagcgtcag caactgctgg cgagccggga gtgcgatatt taccgctctg
     8881 gcaaagacct gttcaacctg tccttgcccc cgctgcgcca gaaaatccgc atccgcagtt
     8941 agcgcccaca cacaccgttt ttctggtgtg ttgatagcca gcctcccggt ttatgcacct
     9001 gcggcaatca gtaactgcgc aatctcttca aagccccgtt cccgcgccag ttccagtggc
     9061 gttttgccat atttatcggt cagatgcggg ctggcaccgt gttccagcaa taactgcaca
     9121 atcgcctgct gtttaatacc accatcatta agcacaatcg cttccagtag cggcgtccag
     9181 cctacatggt tggtctggtt aacgttaatc tccgtatgcg ccaaaagctc ttttacaata
     9241 cttaaatggc ctttttcaca ggcaggcgtc aggccgacac cgccaaaacg ggttacgcaa
     9301 ttaagatcgg gtttagccgg taaaataatt cgtagtagcg ttaaatcatc gttcagacag
     9361 ctaattaaaa aaggatttaa acaggtataa tcttgcttat taatatccgc tccggcatca
     9421 attaatgcct gaacgcaagc atattgctga tataaacttg ccagcgtaat tgccgttttc
     9481 ccctgacgat cgcaggtatt aatatcgaca cccagcgcaa ggcaggtttt tactttatcg
     9541 atatcaccct gttgtgcagc caataaatag tcggcaggta gattttttat actcattcac
     9601 attcccctgg tttttgtggc tgttttcgat gaattgagaa tagcaacgat aatctgataa
     9661 aagaacccgc ttaaatttaa ttcgccgtat ctcgcctttt tgttgaataa tattcaacaa
     9721 tcttttcgca gtgtgcggta gtgcaatatt tgtttctttg acccgatacc ctttcactca
     9781 atcaggtttt atttctctat agtctgcaca tgtaagagta gctttcgtcc atgtctaacc
     9841 agaggggaac aagatgaacg gactcacggc aacgggtgtc accgtcggta tttgtgcagg
     9901 gctttggcag ttagtgtctt cacatgtcgg gctctctcaa ggatgggaat tattaggaac
     9961 cattggcttc gtcgcctttt gcagttttta tgccgcaggc ggcggtaaat cggggtttat
    10021 cagaagtctg gcggttaatt attccgggat ggtgtgggcg tttttcgccg cgctgactgc
    10081 tggttggctg gcctctgtaa gtggactgtc cgcgttctgg gcgagcgtaa ttactaccgt
    10141 acccttctcg gcggtagtgg tctggcaagg gcgtttctgg ttactgtcct ttattcccgg
    10201 tggctttctc ggcatgacgc tgttttttgc cagcgggatg aactggacgg tgacgttact
    10261 gggttttctg gcgggtaact gcgtggggat aatttcagaa tatggtggac aaaaattaag
    10321 cgaagcgacg actaaacgcg acgggtatta aaagttattc aaaaatgatt atctatcttt
    10381 aaccgatacc tctacagtca tagctggaga aattcatagt agataatggc gagaatggtt
    10441 taatgaactc aatttttacc gaagagaatc tgttggcatt tactaccgct gcgcgttttg
    10501 gcagcttcag taaagccgca gaagagttgg gtttaaccac ttccgccatt agctacacca
    10561 tcaagcgtat ggagacgggg ctggatgtgg tgctgttcac tcgcagtacc cgcagcattg
    10621 agttaacgga gtccgggcgt tattttttcc gcaaagctac cgacctgctg aatgattttt
    10681 atgccatcaa acgcagtatc gatactattt cgcagggcat agaagcgcgt gtgcgcattt
    10741 gtattaatca gctgctttat acgcccaaac acaccgcacg cttgttacag gtgctgaaaa
    10801 agcagtttcc tacctgccag attaccgtca ccaccgaagt gtataacggc gtctgggatg
    10861 cgattattaa taatcaggcc aatatcgcca tcggcgcacc cgatacacta ctggacggcg
    10921 gcggtattga ttacactgaa attggcgcga ttcgctgggc ttttgctatc gccccggatc
    10981 acccgttagc ctttgtgccg gaacccatcg ccgaaagcca actgcgtctc taccctaata
    11041 ttatggtgga ggacaccgcg catacgatta ataaaaaagt gggctggttg ctgcacgggc
    11101 aggagtcaat tctggtgcca gatttcaaca ccaaatgtca gtgccagatt ttaggtgaag
    11161 gaattggttt tttgccggat tacatggtcc gtgaggcgat gacgcaatcc ctgctggtta
    11221 cccgacaaat ccataatccg cgccaggatt cgcgcatgtt actggcgact cagcattctg
    11281 ctacgggtca ggtgacgcag tggattaaaa aacaatttgc gccaaatggc atattaacgg
    11341 ggatctatca ggatttactg catcgggaga attaatcgct gccaacctcc cgatgcgctg
    11401 tgaatagcgg tttacatcaa ccacctgctt tcattaccca ttcagagata aatttattac
    11461 ccggtactgg cggagagtac aaatagccct gcaaaaagtg aacgccttta ccgatcatta
    11521 aatccgcctg ctcctgggtt tctacccctt ccgccacgat actcagacca ggcttacgcg
    11581 ctagttcgac aatattgtcc acaatatgac cggagatttc gtcgacactc gccatttgca
    11641 caaatgactt atcgatctta ataaaatcga ccgggaacgc ctgcaagtaa cgataggtcg
    11701 cataacccgt accaaagtca tccagcgcaa aggtaatgtt gtgctgatga aggctgtcaa
    11761 atatcgctct ggcttctggc gttaccggaa tagggttacg ttccgttagc tcgagaacca
    11821 gcttgatttt atcgttacct aatttcttaa ccaggttcag acactctttt tcaaatcccg
    11881 ctgccaaaaa acaacccgcc gagacgttga tgccaatatg gaaattgtcc ggcagcaaat
    11941 gttttaccgg catcagaata tccgcagtct gtttcatcag ttggcgggtc attatgacaa
    12001 taagaccgga tgactccgcc agaggaataa actgatccgg tgggataatt cccgtttgtg
    12061 gatgttccca gcggacaagt acctcacagc ccgtcagtac cccagtctgc gcgcagaaaa
    12121 ccggttggat ccacggtttg aattcgtggt tttccaacgc cagactgatg gcttcaggcg
    12181 tgacgatatg gtgacaacgg ttatcaggga tataaaaatt atttttattt cccgtgccac
    12241 tgatgacttg cggatgatat tgaaaaaaga tatcccgcca gaaggtaata tcactctgga
    12301 tgcctagttt ttcatacagc tgcttctttt gatgtgaaat tgtctttgca ctgcgatttc
    12361 tgtactgtga gatttctgta accgacattc cctccattag cagcttgagt aaaagccgtt
    12421 ctttttcgga cacgatacca ggcaacgtca aattcaccgt tgccccgaac tcttccaaaa
    12481 aaacactcaa atcatgcgaa gccattgaac acaccttgat taaatcttgt gcgccagcta
    12541 aatctgcgag tcttcactct gactgataaa gctggctgca agaaaatgac acagagagcc
    12601 gaattatctc ccgttcggct ctctctcctt ttactcatta ttcagatcag aattgccact
    12661 taatgccaac cattgcagag gtgtcattat atcccctatc ggcaacctga acgcccacat
    12721 tcccccagac attcaggttg gcatttagct gtccttccac cccggttttt atctcagcaa
    12781 tatttcgggc tccatcctga gtgacggaca cgccatccat actggtgctg aaatccttac
    12841 tgttatgcag ccagttcact tctacaaacg gctggaactc gcgggattta ccgtcatcca
    12901 ttttatggtg gctcttcagc caggttttta cgccaagtcg ggtttgaaca ttgccatcac
    12961 cgttgctatg aacgagggtt ccgttgcttt cgcggtgctt atcggctttg actcccatcc
    13021 aggtaacctg tgcttgcggc tgaacatacc attcattacg cgttccctgg ctgccattaa
    13081 attcagctaa tttgtgtttg tatccagctt ccagtgaagc ggtaaatcct tttgatttat
    13141 aggattcact ttgtaagtca tcccctttca ctgtgttatc aaaccagctg tactgcgccc
    13201 aactgtcgag atacgcgcca ttacgcgact cgtcatcggc ataccaggtg gcatagaggc
    13261 cggtgctata tccgttcaca ctcgcttttg cacgataacc ggttcgcgag gaaatggttt
    13321 tgctgtcgct gttgccatat cccgccatga ccccaacatg ccagctgtcg ctgccgtttt
    13381 ggctccactg cgcgacatcg cctcccagtt gcagaacata gcgattgctt tgggttttca
    13441 gctggccgct gccatcacgc catttattat gaccaccttc atggcgcatc cacatagtgg
    13501 tttgtttctg ctcacccgtc accatgtcgg tatagtacgt attacccaga cgctcatgca
    13561 gacgcgtggt gaacattgta ttcgctgctg caaggttcgc aatgtagcta cccgcctccg
    13621 gtcgcaggtc attatccaca ttcagatccg gacccggcgt aggtgtcggg ttagggttcg
    13681 gctctggatt cgggtttggc tccggattcg gcatcgggtc tggctccggc tgcagttcag
    13741 gagaatcact gccgctggtc agataccagt taccactatt tgccccttgt ccacgcgcga
    13801 gagtgtagtc atacgccccc gcaacgatac gaccagcctg aacaaattcg ccctcagact
    13861 taccgtctac atggataagt tcgataccgt taagggtttt cgcacctgta ccgccagcgt
    13921 tattcaccgt aacggcagtc gtgccgctag tatcgccctc gacaaccaat ttatcggtta
    13981 ctgagtcatc gccattcagt accgtattca ttactagttg accgttatta ccgtggtagt
    14041 tccccttcac cactaacaca ttgccagctt gctgtcctga cattccggtc atcagcgtac
    14101 cgttgttggt cagattgccg cccagggtgt aattcacaga cgcagccgct cttttttcag
    14161 cgctattatt caacgccagg gtaccgttgt tctcaacgtt tgcgctaccc aatccctgcg
    14221 catgacttac caggacatta gccgaattag caatgttcac attcgctgtc aggttgctgt
    14281 tatcgcccgc gatgtccagc gtgccgccct gaatattcag ctctccgctc cccgttaacg
    14341 aaccggttga agtgcccccg ttggtaaccg tcagactgcc accatttaag ctcagcagtg
    14401 aatcggcgct gccattgagc gtgcccacgg tctggctgtg accattcata tccagttcag
    14461 tctctgccgc cagacgcagt tcatgggtat tacccaacac gttatcgtta ttcaacaaaa
    14521 gcgtcccact gcgcagatcg gtaacaccgg tatagtcgtt gtctttgtta gaaagcgata
    14581 cggtctgacc cttctggctg ctgaatgcca ggtcaccact gccggtaatc tttgcgctga
    14641 gatcggcggc attctcgctt ttaccgttag agctcagcac cagagcatcg ctgtcggtag
    14701 cgtgtaaatc aagctgggtc aggccgtaac cgatatacag accgtcattg tttaccccac
    14761 tcgtcagacg atagtcgtaa gtgccgacgg ccgctttttg cccattctgc tgaatatcac
    14821 gttgggcact gctggagagg atttgcccat ttttatcctg caattgcaga ttgcccgcat
    14881 cgcccagaac ttccgcacct tgcgcatcaa ccagcttaat ggcgctgtta ccatcatcga
    14941 cttcagtgag cgataacgca gagtcaatat cacgagatac tgagcggaca acgtcactgt
    15001 cagaaacctg aatcacaccc tcgccgcgca aatccagcgt tttactaacg ttgaccgtcc
    15061 cttctgtcat ctgtgcacct gctgtcaggg caccaaaatc aacggttccg ccggagaatc
    15121 ccaaagtgct aatggagtga gcgcccttac ccactgttac caggcttccc tgacccgcag
    15181 tgatcccgcc tctctgcaga aactcagcat tcttttccag gttaaatgtc gtgttctgga
    15241 acgccagtga accctggaac cagtctgtat aagcaaactc atcgaaggag aattcgtgat
    15301 tccccatgtt tatttcaaca atacctctac cttgaagatc gttatcaaat acccaatctt
    15361 tattggcatt gatttgcagt ttgccatcaa catccaggtc cgcacggccg atgttcttgt
    15421 cttcagaaat aaccgcgaca gcaccagaag cgacatcaat atatccgctg aatgcatagt
    15481 tattgccggt cagcgccact gtcccactgt cgatacttat tgtaccgttg ccggaaatct
    15541 tattggcaaa ggtcatgcta tcgtcagcct tcaggtgtaa tttcccgcgg ttgttgattt
    15601 ctgcggtacc tatattttgg gtgatcccta aagcgccatt ttgctcaatt ttgactttac
    15661 cgctaaaggc ggagttatcc ccggttaagt aagtcgtacc attgagcgca ttgatatagc
    15721 ccaggccctc gctgtcactg acaatatccg cggaaatagt cgcgttttta tccgtgtggt
    15781 ttaaggtgat tttcttctcg gatgtctttg cccacacaaa ctcaattttt tcggcatcaa
    15841 taatccctgc agccttcgct gcgcttcctt cctgtgcacc taacgctaac tcagagttgg
    15901 tgcttaaact aatagtcggc gcagagattt taccaccttc cgaaacagca agtgttgtat
    15961 cggagttttg atcataataa cccaggctaa ttttctcact ggcattaact tcaccgccgt
    16021 tattaactga caggtactga ttaccaccgt aactgccgta aacgcccata aagaaggttt
    16081 tagaatttac ctggctgcct tcaccactga catttccctg caaataggtc agacggctgg
    16141 tgcccttaac atggattttg ccgccgttgg agacattaaa atagccgcga tcgtgtctca
    16201 tgcctgagtt tgactcatac aaatcatcag catataactc gctgccagca ttggagacat
    16261 tcaggaaccc cgt
//
LOCUS       DAZPEP010000086        16271 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZPEP010000086 DAZPEP010000000
VERSION     DAZPEP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16271
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.086"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(103..1305)
                     /gene="ubiI"
                     /locus_tag="ACHUT9_002673"
                     /gene_synonym="visC"
     CDS             complement(103..1305)
                     /gene="ubiI"
                     /locus_tag="ACHUT9_002673"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFR1349112.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1328..2506)
                     /gene="ubiH"
                     /locus_tag="ACHUT9_002674"
                     /gene_synonym="visB"
     CDS             complement(1328..2506)
                     /gene="ubiH"
                     /locus_tag="ACHUT9_002674"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFR1349113.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2503..3828)
                     /gene="pepP"
                     /locus_tag="ACHUT9_002675"
     CDS             complement(2503..3828)
                     /gene="pepP"
                     /locus_tag="ACHUT9_002675"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFR1349114.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNESEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3854..4432)
                     /gene="ygfB"
                     /locus_tag="ACHUT9_002676"
     CDS             complement(3854..4432)
                     /gene="ygfB"
                     /locus_tag="ACHUT9_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFR1349115.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4599..4928
                     /gene="zapA"
                     /locus_tag="ACHUT9_002677"
     CDS             4599..4928
                     /gene="zapA"
                     /locus_tag="ACHUT9_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFR1349116.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4970..5153
                     /gene="ssrS"
                     /locus_tag="ACHUT9_002678"
     ncRNA           4970..5153
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHUT9_002678"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5174..5776
                     /locus_tag="ACHUT9_002679"
     CDS             5174..5776
                     /locus_tag="ACHUT9_002679"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFR1349117.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5877..5936)
                     /locus_tag="ACHUT9_002680"
     CDS             complement(5877..5936)
                     /locus_tag="ACHUT9_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFR1349118.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6268..6327)
                     /gene="ibsC"
                     /locus_tag="ACHUT9_002681"
     CDS             complement(6268..6327)
                     /gene="ibsC"
                     /locus_tag="ACHUT9_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFR1349119.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6556..7788)
                     /gene="serA"
                     /locus_tag="ACHUT9_002682"
     CDS             complement(6556..7788)
                     /gene="serA"
                     /locus_tag="ACHUT9_002682"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFR1349120.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8044..8703)
                     /gene="rpiA"
                     /locus_tag="ACHUT9_002683"
     CDS             complement(8044..8703)
                     /gene="rpiA"
                     /locus_tag="ACHUT9_002683"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFR1349121.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            9133..10026
                     /gene="argP"
                     /locus_tag="ACHUT9_002684"
     CDS             9133..10026
                     /gene="argP"
                     /locus_tag="ACHUT9_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFR1349122.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10229..12373
                     /gene="scpA"
                     /locus_tag="ACHUT9_002685"
     CDS             10229..12373
                     /gene="scpA"
                     /locus_tag="ACHUT9_002685"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFR1349123.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            12366..13361
                     /gene="meaB"
                     /locus_tag="ACHUT9_002686"
     CDS             12366..13361
                     /gene="meaB"
                     /locus_tag="ACHUT9_002686"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFR1349124.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13372..14157
                     /gene="scpB"
                     /locus_tag="ACHUT9_002687"
     CDS             13372..14157
                     /gene="scpB"
                     /locus_tag="ACHUT9_002687"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFR1349125.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14181..15659
                     /gene="scpC"
                     /locus_tag="ACHUT9_002688"
     CDS             14181..15659
                     /gene="scpC"
                     /locus_tag="ACHUT9_002688"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFR1349126.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15656..>16271)
                     /locus_tag="ACHUT9_002689"
     CDS             complement(15656..>16271)
                     /locus_tag="ACHUT9_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1349127.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         3951 a         4382 c         4144 g         3794 t
ORIGIN      
        1 cggaggtgaa attagtttat ttcaaatgag gaaaatctcc cggcgaaaaa accgggagat
       61 gaaagtgtga tgggtatcaa ataaacagca gaagagaaat ttttaacgca gccattcagg
      121 caaatcgttt aatcccattg cctggcggat aagttgtggt ttaacgccag gaagcgtgtc
      181 ggccagtttc aaaccaatat cacgcagcag ttttttcgcc ggattggtac cggaaaacag
      241 atcgcggaat ccctgcatac cagccagcat cagcgccgca ctgtgcttgc ggctacgctc
      301 ataccggcgc aggtaaatgt actgcccgat gtctttcccc tgacgatgca accgtttcag
      361 ttcagcaatc agctctgcag catccataaa gccaagattt actccctgcc ccgccagcgg
      421 gtgaatggta tgcgcggcgt cgcccaccag caccagacgg tgcgcggcaa actggcgcgc
      481 ataacgcccc gtcagtggga acacctgacg cgcgctctca accttgcata agcccaggcg
      541 attatcaaaa gcgatattta acgcgcgatt aaattcatct tcacttgcct gctgcatccg
      601 ctgcgcttcc tctggcgaca gtgaccagac aattgagcaa agatgcggat cgctaagcgg
      661 taaaaaggcc agaatgcctt cgccatggaa aacctgccgc gccaccgcat catgcggttc
      721 ttccgtgcga atggtcgcta ccagcgcgtg atgctgataa tcccagaaag tcagcggaat
      781 atctgctttg ttgcgcaacc aggaattagc accgtccgcg ccaatcacca gacgcgccgt
      841 taacatgctg ccatctttca gcgtcaggaa agtttcattt tctccccagg cgacctgctg
      901 taattctgcg ggggccaaca gagtgatatc tgacgactgc tgcgctttgt tccacagcgc
      961 gtagtgaatc actgagtttt caacgatatg cccaagatgg ctatagccca tgctttgatc
     1021 gtcaaacgaa atgtgaccaa aactgtcttt gtcccacact tccataccgt gataacaact
     1081 ggccctacga gagagaatgt cctgccagac gccaagacgg gtgagtaatt tttcgctggc
     1141 ggcattgata gccgaaacgc gcagttgtgg tggtgcatcc gccgccagag gttccggtac
     1201 gcgctgctcc agtacggcaa cgcgtaagcc gctcccctgt aagccgcagg caaccgccag
     1261 ccccaccatg ccgccaccaa caatggctac atcaacactt tgcattgttt attccttaaa
     1321 accgccttca acgcgccacc caaccgaggg tgcgctgcgc cagcacatcg cgtgccgggg
     1381 tgaataattc catcgtcatc agtccgatgt tgcgcccgac aaccagcggt gcccaacggt
     1441 tggcaaaaag atgtaccagg ctgtccgtga cgccgatggt tgcttcgcga tcgctctgtc
     1501 gacgctgctg atagcggcac aatatgccgt aatcaccgat gtcttctccg cgctcatgcg
     1561 cctgagtcag ggtttccgca aggctcatga catctcgcat accgaggtta aacccttgcc
     1621 cggcaatcgg gtgcagagtt tgcgccgcat tgcccaccag cacggtacga tgggtaatag
     1681 ctctggcggc gcgggttaac gccagcggat aagcactgcg tttaccggcg tgggtaattt
     1741 ttccaagtcg ccagccaaag gccgactgga gttcacggca aaacttctcg tcgctccacg
     1801 acagcacctc ttcgcgccgt tccagtggat gacaccagac cagcgaacag cgtccgtcag
     1861 acatcggcaa catcgccagc gggccatgtt gcgtgaagcg ttcaaaagcg cggccttcgt
     1921 gcgcaacgga agtcgcaacg ttggcaatca cggccagttg ttcgtaaggc tcctgctgcc
     1981 agtcaacgcc gcacgcggtg gctaacgctg aatgggtgcc atcagctgct accagcacgc
     2041 ggcccgtcag cgtctcgcca ctctccagcg tcacttcaac gtgactctga gtacgggcaa
     2101 cgttagccac gcgatcaggg caatgcagcg ttacgccagg tgctttacgc agcaatgcaa
     2161 acagccgttg cccgacattg tgcaattcga caacctgtcc cagcgccgcc agttggtaat
     2221 cttctgcggc gagggtgaca aatccagcgt gaccacgatc gctgacatgc acggtagtga
     2281 tggcggttgc gtaatccgcc agagattgcc agacgccgat gcgcgccagt tgctgacagg
     2341 tccccgcagc cagcgcaata gctcgtccat caaagcccgg atgagcatgt gactctggcg
     2401 cggtcgcttc aatcaaatgt accggcagcg ccccgtgact taaccgggaa atagccagcg
     2461 ccagcgtcgc gcccgccatg ccgccaccga cgatgattac gctcattgct ttctcgcagc
     2521 tgccatcaac gcttcgattt cttccggctt tttcaccacg ctggcggtga ggttttcgtt
     2581 gccggtttcg gtaatcacaa tgtcgtcttc aatacgaatg ccgataccgc gatattgttc
     2641 tggcacatcc gcatccggcg cgatatacag accaggctct acggtcagta ccatccccgg
     2701 ttccagaatg cgcgagcgat cctgaccata aacgccaacg tcatggacat ccagtcctaa
     2761 ccagtggcta aggccatgca taaagaaagg acgatgggcg ttctgagcga tcagttcatc
     2821 aacatcacct ttcaggatgc cgagttttac caggccgcta accatgatgc gcaccacttc
     2881 accagtgact tccagaatgg aagttcccgg acgatacagg cgcaggctgg tttcgagaga
     2941 ctccagcaca atgtcgtaga tttcacgctg ggcctgggtg aatttgccgt tgaccgggaa
     3001 ggtgcgggta atatcgcccg cgtaaccttt gtattcacaa cccgcgtcaa tcaacaccag
     3061 gtcaccgtcg cgcagttcac tctcgttttc ggtgtagtgc agaatgcagc cgttttcacc
     3121 gctgccgaca atggtgttat aggacggata gcgcgcaccg tggcggttaa attcgtggtg
     3181 aatttcgcct tccagatggt actcgaacat tcccggacgg catttttcca tcgcccgtgt
     3241 atgtgccatg gcggtgattt ctcccgcgcg gcggagtacg gcaatctctt ctggcgattt
     3301 gaacaggcgc atttcatgaa caacaggacg ccagtcgatc atcgttgccg gtgcggtgag
     3361 attttgccgc gaacctttac gcagtttttc cagcgcactg ttcatgattt catcagcata
     3421 tgcatattcg ccctgggcat ggtaaaccac atccaggccg ttaagtagtt gataaagttg
     3481 ctgattgatt tcgctgaatg ccagtgcgcg gtcaacgccc agtttctctg gcgcggcatc
     3541 ctggcctaag cgacggccaa accagatctc cgccgtcagg tcgcgaacgc ggttaaacag
     3601 aacgctgtgg ttatgagtgt catcgctttt aatcagcacc agcaccgctt ccgattcgtt
     3661 aaagccggtg aagtaccaga agtcactgtt ctgacgatag gggtattcgc tgtcggcgct
     3721 acgtgttact tctggtgcag caaaaatcag cgcggcgctg ccgggttgca tttgctccac
     3781 cagggcctga cggcgacgct gaaactcttg ccgggatatc tcactcataa cactctcctt
     3841 acgttttttg tttttagtgt agagtcggtt tttgtacttc tggcgcggtc ggttgcggat
     3901 gagtaaaggt gtcgtggcat aacagcgcgg caacacgaac gtattcgatg atctcttcaa
     3961 gcgacatttc aagctcttcc tgatcttcgt cttcgtcgta acccagttgc gcaatgttac
     4021 gcagatcgtc gatagcttca ccggtttcgc cggtcacttt atccagcttc ggttgcgtaa
     4081 cgccaagacc aagcaggaag tgattgaccc aacctgccaa tgcatcagcc cgatcgaaaa
     4141 cgctgacatc atcgccatca ggcagataaa gctgaaaaag gaagccgtca tcctgcaggg
     4201 catcgctggt ggcagagtgc attttacgca gtgcctgtgc cagctcatga ccgaaagcca
     4261 tgccttcgtt cgtcaggtcg tgaagtagcg gcagccatga gctgtcatcg ttaccgccac
     4321 atatcatccc actgattaaa ccatgcatct cggcaggggt cagccccgtc ccttgttggt
     4381 tcagatactg gttcatttcg ttgtaaccag gcatttcgtt ctgtatagac ataagcattc
     4441 gtcgtcaaag ggaggaatat tcatgatatg ctaccacttt gggccctggt ggaccagaaa
     4501 agggcttgtc tcttctcatc agggtagcta tagtgtcgcc cttcgcagac catgggtcta
     4561 aagacgaagg cagcgcagtc aatcagcagg aaggtggcat gtctgcacaa cccgtcgata
     4621 tccaaatttt tggccgttca ctgcgtgtga actgcccgcc tgaccaaagg gatgcgttga
     4681 atcaggcagc ggacgatctg aaccaacggt tgcaagatct gaaagaacgc actagagtca
     4741 caaatactga acagttggtc ttcattgccg cattgaatat cagctatgag ttagcgcaag
     4801 aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca gcgtattcgg atgctgcagc
     4861 agaccataga acaagcgtta cttgaacaag gtcgcatcac cgaaaaaact aaccaaaact
     4921 ttgaatgaca cttttcggtt tactgtggta gagtaaccgt gaagacaaaa tttctctgag
     4981 atgttcgcaa gcgggccagt cccctgagcc gatatttcat accacaagaa tgtggcgctc
     5041 cgcggttggt gagcatgctc ggtccgtccg agaagcctta aaactgcgac gacacattca
     5101 ccttgaacca agggttcaag ggttacagcc tgcggcggca tctcggagat tcccttctta
     5161 tctggcacca gccatgacgc aactaccaga actcccactg acattatccc gacaagaaat
     5221 ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg gaacaacagc aggaaatggg
     5281 tcaacaagcc gctacccgga tgatgactta tcccccggtg gtgatggcgc atacggtcgc
     5341 tgtattcctc tcttttgatg gtgaactcga cacccagccg ctaatagaac aactctggcg
     5401 cgccggtaag cgcgtatatc ttccagtttt gcatcccttt agtgccggta atttgctgtt
     5461 cctgaattac catccgcaaa gcgaactggt gatgaacagg ttgaagatcc atgagccaaa
     5521 actggatgtg cgtgacgtgc tacccctttc ccgattagac gtgctgatca caccgctggt
     5581 cgcctttgat gagtacggtc agcgcctggg aatgggcggt ggtttttatg atcggacctt
     5641 acaaaactgg cagcactata aaacgcaacc ggtgggttat gcgcatgatt gtcagttggt
     5701 ggaaaaactc cccgttgaag agtgggatat ccctcttcct gcggtggtta caccgtcgaa
     5761 agtctgggag tggtaagggc gatacacctg catcgccctg attgacatcg ttgattcttt
     5821 aacctaattt agtgagtaag ggtaagggag gattgctcct cccctgtgac tgactgttaa
     5881 taagctggga aacttatgag taacagtaca atcagtatga tggcaagtcg catcataacc
     5941 cttctccttc aagccctcgc ttcggtgagg gctttaccgt tacagcccca tgctgcccag
     6001 ctatcgtaag ctctctttaa ataaccacaa cgcattgatc tacctttaaa tcttttcttc
     6061 gagcgtgctc gaaaaaccca tgcaaaacag agataatttt tgggaaaaag gatctattac
     6121 ggcagtaaac gtgggcgatg aagtggctat caaaaagttc ggttcagggc gatgcacccc
     6181 gcatcgccct gattgacatc gttgattctt tgacctaatt tagtgagtaa gggtaagaga
     6241 ggatgactcc tcccctgaga ctgactgtta ataagctccg aaacttatca gtaacagtac
     6301 aatcagtatg atgacaagtc gcatcataac ccttctcctt taagccctcg cttcggtgag
     6361 ggctttaccg ttacagcccc atgctgtccc gccatcgtaa atccccatta aataaacaca
     6421 acgcattgat ctgagtttga tttattttct ggagcagact cgcaaagtag aatgcgcaac
     6481 gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt cagtgacctg cccgttaatt
     6541 ttcagagaag gggaattagt acagcagacg ggcgcgaatg gtacccggaa tagctttcat
     6601 tgcctgcagc gctttttcgg caacgtcttc gtcagcttca atatcaataa ctacataacc
     6661 catctgggcg gaagtttgta gatattgcgc ggcgatgttg acgccctgct cggcgaagat
     6721 tttgttcagc gcagttagca cgcccggacg gttttcgtgg atgtgcatca gacgacgacc
     6781 accatgcagt ggcagcgaga cttccgggaa gttcaccgca gagagcgttg agccattgtc
     6841 agaatacttg atcaatttac ccgcaacttc caggccgata ttctcctgcg cttcctgagt
     6901 cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat tcacacagtg gagaggtaaa
     6961 tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg attgccgccc ccgccagatg
     7021 tttgctcgcc agcgcatcac acagcgccgg aatatccacc acagtaccgc gcgaggcatt
     7081 aatcagcagc gagccgggct tcattagcga aatctctttc gcgcccatca tatttttggt
     7141 ggacggattc tctggtacat gcagactcac cacatcgctc atattcagca ggtcagaaag
     7201 atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt tcaatatcat aaaagtaaac
     7261 atacattccc agcgattcag ccagaatgcc caattgcgta ccaatatgac cgtagccgat
     7321 gatacccagc tttttgccgc gcgcttcaaa agaacccgcc gccagtttgt tccacacgcc
     7381 acggtgcgct ttagcattgg cttccggcac gccgcgcaat agcagcagca gttcgccaat
     7441 caccagctcc gcaacagagc gcgtatttga gaacggtgcg ttaaataccg ggatcccgcg
     7501 ctttgccgcc gcatccagat caacctggtt tgttccgata cagaaacagc caatagcgac
     7561 cagtttttct gcggcgttga tcacgtcttc agtcagatgg gtacgggatc gcaggccgat
     7621 gaagtgggca tcgcggatgg attcttttaa ttgttcatca tccagcgcgc ctttgtgaaa
     7681 ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc gccttttggt gcacgccttc
     7741 taccagcaga aacttaatct tgtctttctc cagcgatacc tttgccattt acccaatcct
     7801 gtcttttgaa atgttgtgtg cggatttgca tccgcctttc aacatatcaa aaaataatat
     7861 tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat atcacgcaaa ggtatttctt
     7921 ggttgaatat gctggtttag aggatttatt tagagcaatc gacaatagcc aagtaaaaac
     7981 gtgacacatg tcaccaaatt taatgaagag aattttttta acgggggagg ttcccccgtc
     8041 agatcatttc acaatggttt tgacaccgtc aggtgtgcca atcagcgcaa cgtccgcgcc
     8101 acggttagca aacaagccaa cagtcaccac gccaggaatc gcatttatgg cgttttccat
     8161 cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg atcacgttgc cgttatcggt
     8221 caccacgccc tgacggtatt ccggacgacc gcccagtttc accagctggc gcgccactgc
     8281 actacgtgcc atcgggataa cttctactgg cagcgggaat ttgcccagaa tatcaacctg
     8341 cttggaagcg tcagcaatac agataaattt ttccgcaacc gaagcaatga ttttttcacg
     8401 ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg ccgttgattt catctgcgcc
     8461 atcaacgtag atgccaaggc tgtcgacttc gttgagatca aaaacgtgaa tgccgaggct
     8521 tttcagtttt tcagtggaag catctgaact ggaaacggcc ccttcaatct ggcctttcat
     8581 tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct gtacctacac caacaatggt
     8641 gccgggctga acatactgaa gtgccgccca tcctactgct tttttcaatt catcctgcgt
     8701 catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt atatacaaaa aaagcgattc
     8761 agaccccgtt ggcaagccgc gtggttaact catccataaa atatcgcgca atggcgggca
     8821 tcccctttcg ccccgcaaat aaagcataca acggtctggg tatgccgctc cacggtgcga
     8881 acaggcgcac cagttccccc cgttcgcaag cccctgttta caggcaaatt gaggcaataa
     8941 cgccacccca ttcaacacaa ccagggcacg aactcgctga gcgaaatgca ttagcgcaaa
     9001 tccctgatct tttaagctgc gcgtttaacc acggatacaa atgttacccg ccgacggctt
     9061 cggtatatgc aacctgacac aaaattgtgt catagtgcag gaaaaagcat ttaccaggag
     9121 cagacaacag caatgaaacg cccggactac agaacattac aggcactgga tgcggtgata
     9181 cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca ttacacaatc agccgtctca
     9241 cagcgcatta agcaactgga aaatatgttc gggcagccgc tgttggtgcg taccgtaccg
     9301 ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc tgcgccaggt ggagttgctg
     9361 gaagaagagt ggctgggcga tgaacaaacc ggttcgactc cgctgctgct ttcactggcg
     9421 gtcaacgccg acagtctggc gacgtggttg cttccggcac tggctcctgt gttggctgat
     9481 tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc gtactcagga acgtctgcgc
     9541 cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg cgctgccgag ttgtcttgtc
     9601 gataaacttg gtgcgctcga ctatctgttc gtcagctcaa aaccctttgc cgaaaaatat
     9661 ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac ctgtggtcgc ctttgaccat
     9721 cttgacgata tgcaccaggc ctttttgcag caaaacttcg atctgcctcc aggcagcgtg
     9781 ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac ttgctcgcca gggcaccacc
     9841 tgctgtatga tcccgcacct gcaaatcgag aaagaactag ccagcggtga actgattgac
     9901 ttaacgccag ggctatttca acgccggatg ctctactggc accgctttgc tcctgaaagc
     9961 cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg gtcacaaagt ccttcgtcag
    10021 gattaatcca tcaaataatg cctgatagca catatcaggc gttatcctca cttctttttg
    10081 tattccttga atcacatcac aaaatagaca aatctcaggc ggcaaaaaac gacgtctgaa
    10141 tgcatttttt tgctggcgac aaacccacgt aaaaagctca ccgtaagcgc aaataccctc
    10201 attttgattg cgttttacgg agcaaataat gtctaacgag caggagtggc aacagcttgc
    10261 caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg ctggttcagc aaaccgcgga
    10321 agggatcgcc atcaagcagc tgtataccga agccgatctc gataatctgg aggtgacagg
    10381 tacccttcct ggtttgccgc cctacgttcg tggcccgcgt gccactatgt ataccgccca
    10441 accgtggacc atccgtcagt atgctggttt ttcaacagca aaagagtcca acgcttttta
    10501 tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt gcgtttgacc ttgccaccca
    10561 ccgtggctac gactccgata acccacgcgt ggcgggtgac gtcggcaaag cgggcgtcgc
    10621 tatcgacacc gtggaagata tgaaagtcct gttcgaccag atcccgctgg ataaaatgtc
    10681 ggtttcgatg accatgaatg gcgcagtgct accagtactg gcgttttata tcgtcgccgc
    10741 agaagagcaa ggtgttacac ctgataaact gaccggcacc attcaaaacg atattctcaa
    10801 agagtacctc tgccgcaaca cctatattta cccaccaaaa ccgtcaatgc gcattatcgc
    10861 cgacatcatc gcctggtgtt ccggcaacat gccgcgattt aataccatca gtatcagcgg
    10921 ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag gtagcattta cgctcgccga
    10981 tgggattgag tacatcaaag cagcaatctc tgccggactg aaaattgatg acttcgctcc
    11041 tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt atgaacgtcg ccatgttgcg
    11101 tgcggcacgt tatttatgga gcgaagcggt cagtggattt ggcgcacagg atccgaaatc
    11161 actggcgctg cgtacccact gccagacctc aggctggagc ctgactgaac aggatccgta
    11221 taacaacgtt atccgcacca ccattgaagc actggctgcg acgctgggcg gtactcagtc
    11281 actgcatacc aacgcctttg acgaagcgct tggtttgcct accgatttct cagcacgcat
    11341 tgcccgcaac acccagatca tcatccagga agaatcagaa ctctgccgca ccgtcgatcc
    11401 actggccgga tcctattaca ttgagtcgct gaccgatcaa atcgtcaaac aagccagagc
    11461 tattatccaa cagatcgacg aagccggtgg catggcgaaa gcgatcgaag caggtctgcc
    11521 aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg ctgatcgacc aaggcaagcg
    11581 tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa gacgaaaccg atgtacttga
    11641 gatcgacaac gtgatggtgc gtaacgagca aattgcttcg ctggaacgca ttcgcgccac
    11701 ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg actcacgccg cacagcataa
    11761 cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt cgcgccaccc tgggtgaaat
    11821 ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg ccaagccagt gtgttaccgg
    11881 cgtgattgcg caaagctatc atcagtctga gaaatcggcc tccgagttcg atgccattgt
    11941 tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc ccgcgcattc tgatcgctaa
    12001 gatgggccag gatggacacg atcgcggcgc gaaagtgatc gccagcgcct attccgatct
    12061 cggtttcgac gtagatttaa gcccgatgtt ctctacacct gaagagatcg cccgcctggc
    12121 cgtagaaaac gacgttcacg tagtgggcgc atcctcactg gctgccgatc ataaaacgct
    12181 gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc gaagatatct gcgtggtcgc
    12241 gggtggcgtc attccgccgc aggattacgc cttcctgcaa gagcgcggcg tggcggcgat
    12301 ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac gtactgaatc tgataagcca
    12361 gcatcatgat taatgaagcc acgctggcag aaagtattcg ccgcttacgt cagggtgagc
    12421 gtgccacgct cgcccaggcc atgacgctgg tggaaagccg tcacccacgt catcaggcgc
    12481 taagtacaca gctgcttgac gccattatgc cgttctgtgg taacgccctg cgactgggcg
    12541 ttaccggcac acccggcgcg gggaaaagta cctttcttga agcctttggc atgttgttga
    12601 ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc cagcagcccg gtcactggcg
    12661 gtagcattct cggggataaa acccgcatga atgacctggc gcgtgccgag gcggcgttta
    12721 ttcgcccggt accctcctcc ggtcatctgg gcggtgccag tcagcgagca cgggaattaa
    12781 tgctgttatg cgaagcggcg ggttatgacg tagtgattgt cgaaacggtt ggcgtcgggc
    12841 agtcggaaac ggaagtcgcc cgcatggtgg actgttttat ctcgttgcaa attgccggtg
    12901 gcggcgatga tctgcagggc attaaaaaag ggctgatgga agtggctgat ctgctcgtta
    12961 tcaacaaaga cgatggcgat aaccatacca atgtcgccat tgcccggcat atgtacgaga
    13021 gtgccctgca tattctgcga cgtaaatacg acgaatggca gccacgggtt ctgacttgta
    13081 gcgcactgga aaaacgtgga atcgatgaga tctggcacgc catcatcgac ttcaaaaccg
    13141 cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca acaatcggtg gaatggctgc
    13201 gtaagcagac cgaagaagaa gtactgaatc acctgttcgc gaatgaagat ttcgatcgct
    13261 attaccgcca gacgctttta gcggtcaaaa acaatacgct ctcaccgcgc accggcctgc
    13321 ggcagctcag tgaatttatc cagacgcaat attttgatta aaggaatttt tatgtcttat
    13381 cagtatgtga acgttgccac tatcaacaaa gtggcggtca ttgagtttaa ctatggacga
    13441 aaacttaatg ccttaagtaa agtctttatt gatgatctta tgcaggcgtt aagcgatctc
    13501 aaccggccgg aaattcgctg tatcattttg cgggcgccaa gtggatccaa agtcttctcc
    13561 gcaggtcacg atattcacga actgccgtct ggcggtcgcg atccgctctc ctatgatgat
    13621 ccattgcgtc aaatcacccg catgatccaa aaattcccga aaccgatcat ttcgatggtg
    13681 gaaggtagtg tttggggtgg cgcatttgaa atgatcatga gttccgatct gatcatcgcc
    13741 gccagtacct caaccttctc aatgatgcct gtaaacctcg gcgtcccgta taacctggtc
    13801 ggcattcaca acctgacccg cgacgcgggc ttccacattg tcaaagagct gatttttacc
    13861 gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca tcctcaacca tgttgtggaa
    13921 gtggaagaac tggaagattt caccttacaa atggcgcacc acatctctga gaaagcgccg
    13981 ttagccattg ctgttatcaa agaagagctg cgtgtactgg gcgaagcgca caccatgaac
    14041 tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg tttatgacag cgaagattac
    14101 caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta atttcgttgg tcattaatcc
    14161 ctgcgaacga aggagtaaaa atggaaactc agtggacaag gatgaccgcc gatgaagcgg
    14221 cagaaattat ccagcataac gacatggtgg catttagcgg ctttacccca gcgggttcgc
    14281 cgaaagcctt accgaccgcg attgcccgca gagctaacga acagcatgag gccaaaaagc
    14341 cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc cgccgctgac gatgtacttt
    14401 ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac atcgtccggt ttacgtaaaa
    14461 agatcaatca gggcgcggtg agtttcgttg acctgcattt gagcgaagtg gcgcaaatgg
    14521 tcaattacgg tttcttcggc gacattgatg ttgccgtcat cgaagcatcg gcactggcac
    14581 cggatggtcg agtctggtta accagcggga tcggtaatgc gccgacatgg ctgctgcggg
    14641 cgaagaaagt gatcattgaa cttaatcact atcacgatcc gcgcgttgca gaactggcgg
    14701 atattgtgat tcctggcgca ccaccgcggc gcaatagcgt gtcgatcttc catgcaatgg
    14761 atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa gattgtcgcc gtcgtggaaa
    14821 ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa tcccatgtgc cagcagattg
    14881 ccgataacgt ggtcacgttc ttattgcagg aaatggcgca tgggcgtatt ccgccggaat
    14941 ttctgccgct gcaaagtggc gtgggcaata tcaataatgc ggtaatggcg cgtctggggg
    15001 aaaacccgga aattcctccg ttcatgatgt attcggaagt gctacaggaa tcggtggtgc
    15061 atttactgga aaccggcaaa atcagcgggg ccagcgcctc cagcctgaca atctcggccg
    15121 attccctgcg caagatttac gacaatatgg attactttgc cagccgcatt gtgttgcgtc
    15181 cgcaggagat ttccaataac ccggaaatca tccgtcgtct gggcgtcatc gctctgaacg
    15241 tcggcctgga gtttgatatt tacgggcatg caaattcaac gcacgtagcc ggggtcgatc
    15301 tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa cgcgtatctg tcgatcttta
    15361 tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt cgtgccaatg tgcagccatg
    15421 ttgatcacag cgaacacagc gtcaaagtga tcatcaccga acaagggatc gccgatctac
    15481 gcggtctttc cccgcttcaa cgcgcccgca ctatcattga taattgtgca catcctatgt
    15541 atcgggatta tctgcatcgc tatctggaaa atgcgcccgg cggacatatt caccacgatc
    15601 ttagccacgt cttcgactta caccgtaatt taattgcaac cggctcgatg ctgggttaat
    15661 ggtttgtccc gtcacgatct tcagcaatat gctgaagcat cgtggcggta taacgatgat
    15721 ttctgattga ataaagatat tcctgcatat acatcggcct gtgcggcgca tcaaaatatt
    15781 tcagcgttgc cggattcacc agtcgccagg caaaatgcgg gatcaccgtc agaaaatgtc
    15841 cgcgctccac ggcgctgatt ttggccataa aactgtaggg acgataaata atggtgggat
    15901 tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc gaagttagcc cggttttgaa
    15961 agcgcatttg tagccagggc agctcctgaa gcagctcttg ctcttgacgc tcttcataac
    16021 ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc actgatagtc aggttttcca
    16081 gttcctgaac tcgtgcagat acatgctgtg gggagataat aaaatctaac tgacggtcaa
    16141 aaagattatc gataacgccg ttttcgctga actcaactgg ctgggcggtc acgccttcat
    16201 acttatctcc gagactaatc aactgatcga aaataattgt cggataagta ttatctatgc
    16261 caatcacaat t
//
LOCUS       DAZPEP010000087        16245 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZPEP010000087 DAZPEP010000000
VERSION     DAZPEP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16245
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.087"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..578
                     /locus_tag="ACHUT9_002690"
     CDS             <1..578
                     /locus_tag="ACHUT9_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFR1349128.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENCYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            581..1744
                     /locus_tag="ACHUT9_002691"
     CDS             581..1744
                     /locus_tag="ACHUT9_002691"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFR1349129.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1828..3453
                     /locus_tag="ACHUT9_002692"
     CDS             1828..3453
                     /locus_tag="ACHUT9_002692"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFR1349130.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHUT9_002693"
     CDS             complement(3570..3845)
                     /gene="yjfN"
                     /locus_tag="ACHUT9_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFR1349131.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHUT9_002694"
     CDS             complement(3994..4323)
                     /gene="bsmA"
                     /locus_tag="ACHUT9_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFR1349132.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHUT9_002695"
     CDS             4505..5254
                     /gene="yjfP"
                     /locus_tag="ACHUT9_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1349133.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHUT9_002696"
     CDS             complement(5251..6006)
                     /gene="ulaR"
                     /locus_tag="ACHUT9_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFR1349134.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHUT9_002697"
     CDS             complement(6114..7178)
                     /gene="ulaG"
                     /locus_tag="ACHUT9_002697"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFR1349135.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHUT9_002698"
                     /pseudo
     CDS             7533..8931
                     /gene="ulaA"
                     /locus_tag="ACHUT9_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHUT9_002699"
     CDS             8947..9252
                     /gene="ulaB"
                     /locus_tag="ACHUT9_002699"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFR1349136.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9262..9723
                     /gene="ulaC"
                     /locus_tag="ACHUT9_002700"
     CDS             9262..9723
                     /gene="ulaC"
                     /locus_tag="ACHUT9_002700"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFR1349137.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVADVVEPRYYQ
                     AILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDILI
                     TMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9737..10382
                     /gene="ulaD"
                     /locus_tag="ACHUT9_002701"
                     /pseudo
     CDS             9737..10382
                     /gene="ulaD"
                     /locus_tag="ACHUT9_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
     gene            10392..11246
                     /gene="ulaE"
                     /locus_tag="ACHUT9_002702"
     CDS             10392..11246
                     /gene="ulaE"
                     /locus_tag="ACHUT9_002702"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFR1349138.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11246..11932
                     /gene="ulaF"
                     /locus_tag="ACHUT9_002703"
     CDS             11246..11932
                     /gene="ulaF"
                     /locus_tag="ACHUT9_002703"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFR1349139.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12029..12580
                     /locus_tag="ACHUT9_002704"
     CDS             12029..12580
                     /locus_tag="ACHUT9_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFR1349140.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12655..12930)
                     /gene="yjfY"
                     /locus_tag="ACHUT9_002705"
     CDS             complement(12655..12930)
                     /gene="yjfY"
                     /locus_tag="ACHUT9_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFR1349141.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13257..13652
                     /gene="rpsF"
                     /locus_tag="ACHUT9_002706"
     CDS             13257..13652
                     /gene="rpsF"
                     /locus_tag="ACHUT9_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFR1349142.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13659..13973
                     /gene="priB"
                     /locus_tag="ACHUT9_002707"
     CDS             13659..13973
                     /gene="priB"
                     /locus_tag="ACHUT9_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFR1349143.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            13978..14205
                     /gene="rpsR"
                     /locus_tag="ACHUT9_002708"
     CDS             13978..14205
                     /gene="rpsR"
                     /locus_tag="ACHUT9_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFR1349144.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14247..14696
                     /gene="rplI"
                     /locus_tag="ACHUT9_002709"
     CDS             14247..14696
                     /gene="rplI"
                     /locus_tag="ACHUT9_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFR1349145.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14808..15779)
                     /locus_tag="ACHUT9_002710"
     CDS             complement(14808..15779)
                     /locus_tag="ACHUT9_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1349146.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15832..>16245)
                     /locus_tag="ACHUT9_002711"
     CDS             complement(15832..>16245)
                     /locus_tag="ACHUT9_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1349147.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3817 a         3967 c         4319 g         4142 t
ORIGIN      
        1 ttggtagacc gaataatcct tttgaaccgc gtcgcaatcg ctacgcacaa aaatacttaa
       61 cgttggcgct aatgggcggt gccgcttttt tcgtattgaa aggttgtggc gatagtagcg
      121 atgtcgataa cgacggcgac ggaacgtttt acgcgacagt gcaggattgt attgatgacg
      181 gtaataatgc cgacatttgc gcgcgtggct ggaacaacgc caaaacggca ttttatgccg
      241 atgttccgaa gaatatgact cagcagaact gtcagtctaa gtacgaaaat tgctactacg
      301 acaatgttga acagagttgg atcccggtcg tttctggatt tttgttaagc cgggttattc
      361 gtaaagatcg cgatgagccg ttcgtctata acagcggcgg atcctcattt gcttcccgcc
      421 ccgtctggcg caacacttcc ggtgattact cctggcgctc cggttctggc aaaaaagagt
      481 cttactcttc aggcggcttt accaccaaaa aagcgtcaac cgtttcgcgc ggcggctatg
      541 gtcgttcttc cagcgcccgt gggcattggg gaggctaatc atgctgagac acaacgttcc
      601 tgtgcggcgg gatctggacc aaattgccgc tgacaacggt ttcgactttc atatcatcga
      661 caatgaaatc tattgggacg agagtcgggc ttaccgtttt actctgcgcc agattgaaga
      721 gcagatcgaa aaaccaactg cggaactgca tcagatgtgc cttgaggtgg tggatcgcgc
      781 ggttaaagac gaagagatcc tgacgcaact ggcgatcccg ccgttgtact gggatgtgat
      841 cgccgaaagc tggcacgcca gcgatccttc gctgtatggc cggatggatt ttgcctggtg
      901 tggcaatgcg ccggtgaagc tgctggagta caacgccgat acgccgactt cgttgtatga
      961 gtcggcttat ttccagtggt tgtggctgga ggatgcccgg cgcagcggca ttattccgcg
     1021 tgatgccgat cagtacaatg ccattcagga acgcctgatt tcacgcttta gcgagcttta
     1081 tagccgggag ccgttttatt tctgctgctg tcaggacacc gatgaagaca ggactaccgt
     1141 gctgtacttg caggactgcg cccagcaggc agggcaggag tcgcggttta tctacattga
     1201 agatctcggt ttgggcgtcg gcggtgtact gaccgatctt gatgataatg tcatccagcg
     1261 tgcatttaag ctgtatccgc tggagtggat gatgcgtgac gataacggtc cgctgctgtg
     1321 caagcgtcgc gagcaatggg tggagccgtt atggaaaagt atcttgagta ataaagggct
     1381 aatgccgctg ctttggcgct tcttccctgg tcatcctaac cttcttgcat cctggtttga
     1441 gggtgaaaaa tcgcagattg ccgctggcga aagctatgtg cgtaaaccga tctactcgcg
     1501 cgaaggcgga aacgtcacca ttttcgacgg tcagaataac gtcgttgacc acgctgatgg
     1561 tgattacgcc gatgaaccga tgatctacca ggcgtttcaa cctctgccgc gatttggcga
     1621 tagctacaca ctcatcgata gctggattgt cgatgatgaa gcgtgcggaa tggggatccg
     1681 tgaagataac acactgatca ccaaagacac ctcacgtttc gttccgcatt acattgctgg
     1741 ataagaatgt tttagcaatc tctttctgtc atgaatccat ggcagtggcc atactaatgg
     1801 tgactgccat tgatggaggg agacacagtg cactggcaaa ctcacaccgt ttttaatcaa
     1861 cctataccat taaataacag caacttatac ctgtctgatg gcgcgctttg cgaagcggta
     1921 acgcgtgaag gtgctggctg ggatagcgat tttctagcca gtattggtca gcagttagga
     1981 acggctgaat cccttgaact ggggcggctg gcgaatgtaa acccgcctga actgttgcgc
     2041 tacgatgccc aggggcggcg tctggacgat gttcgtttcc atcccgcatg gcacctgctg
     2101 atgcaggtgt tatgtaccaa tcgggtgcac aatcttgcct gggaagaaga cgctcgctcc
     2161 ggcgcatttg tggcgcgcgc ggcgcgtttt atgttacatg cgcaggttga ggctgggtcg
     2221 ttatgtccaa taacgatgac ctttgccgcc acgccattgt tgttacagat gttacccacg
     2281 ccgtttcagg actggaccac gccgctgctg agcgatcgct acgattctca cttattgcca
     2341 ggtgggcaaa aacgcggttt gttgattggc atgggaatga cggaaaagca gggtggttcc
     2401 gatgtcatga gcaacaccac ccgtgcagag cgtttggaag atggctctta tcggctggtg
     2461 gggcataaat ggtttttctc ggttccgcaa agcgatgcgc atctggtgct ggcgcagacc
     2521 acgggcggtc tgtcctgttt ttttgtgccg cgctttttgc ctgacgggca acgcaacgcg
     2581 attcgcctcg aacggctgaa agataagctg ggtaatcgct ctaacgccag ttgtgaagtg
     2641 gagtttcagg atgccattgg ctggttattg gggcaggaag gggaaggaat tcgtctgatc
     2701 ctgaaaatgg gggggatgac gcgttttgat tgcgccctgg gtagccatgc catgatgcgc
     2761 cgtgcgtttt cgctggcgat ttatcatgca catcaacgcc atgtttttgg taatccattg
     2821 atccaacagc cccttatgcg tcatgtctta agtcgcatgg cacttcagct tgaagggcaa
     2881 acggcgttgc tgtttcgtct tgcgcgagcg tgggaccggc gcgccgatgc caaagaagcc
     2941 ctgtgggcgc gtttatttac gcctgcggca aaatttgtga tctgcaaacg aggtatgccg
     3001 tttgtggccg aagcgatgga ggtgctgggc ggcattggtt attgcgagga gagcgagctg
     3061 ccgcggcttt accgggagat gccggtaaac agtatttggg aaggttccgg caatattatg
     3121 tgcctggatg tgttgcgcgt tctcaataag caagcgggcg tatacgactt attgtcggaa
     3181 gcatttgtgg aagtgaaagg gcaggatcgc tattttgatc gcgcggttcg tcgtttacag
     3241 cagcagctgc gtaagccagc tgaagaactg gggcgagaga ttactcatca gctattcctg
     3301 ctgggctgcg gtgcgcaaat gttgaaatat gcttctccgc caatggcgca ggcgtggtgt
     3361 caggtgatgt tagatacgcg cggcggcgta cggttgtcag agcagatcca gaatgattta
     3421 ttgctgcggg cgacgggggg agtgtgtttg taaacgtata cgactgatgc gacgctggtt
     3481 tcgattaact caatgaaata tgtgaaaatt gtaggccgga caaggcgctc gcgccgcatc
     3541 cggcattgtt catttgctgt agatgacact catgcataca gtatcgcctg tacgcgccag
     3601 ttatcaggct gctggtcttc gtacatggta ataatgcggt accatgacgc accttgttca
     3661 tcggccttta acgccacgat acgttccacg tcctgcggat cccctgaaat attgctaact
     3721 gagatctgac ctatttcatt caggcccgtc acgcagtcag cactggcgaa ttctgcagac
     3781 tgtgccgtgg cgctcgcagg ataagtcact aattgcagcg agggtgaggc aagtaattgt
     3841 ttcatcgtca gctccatttt tcgtttcccc acatgttgca gggcattcca tcgcggtaat
     3901 aaacgcccag gcttccagcc ggtaatttat tgtgcacagg taaacgcata gggacgcaaa
     3961 gcaatgtaaa gctgactatg taagttgtct gatttattta cggtacaaaa tggcttgtga
     4021 ataccactgt cctgtcacga tggtttcgtc caccatgacg acaacgtaat aatcagcttt
     4081 tgcagcgaca gcttttgctc tgatttctgc taatgcgtca tccggagaac cccgaaccat
     4141 cgtgcttacg ctacctattc gctgtaaccc ttgcgtctgg tcgcggcgaa tctcttgcgg
     4201 atggtccgtt actggcggtg ctggctgtgg cgtaccttgc agtgcgctac aggcacttaa
     4261 catcaacacc aataataaac tggcaaaccg gtaaataacg ctattacgtt tcctgctaac
     4321 catagtgtag tgccttatta atttaacttt gggggaatgt tcccgaattg ttacctggta
     4381 cgcgattttc gaatgaaaac gtgacgaaag cgtaacccac atctcaacat tatgaactaa
     4441 taccgccagc agaaacttgt gctaaggatt tcttcatgtc ataccagaca aaaaggagag
     4501 acagatgatt gaaatagaat cacgcgagct ggcagatatt cccgttcttc atgcttatcc
     4561 tgtcgggcaa aaagataccc cgttaccgtg cgtaattttt tatcacggct ttacttcatc
     4621 cagcctggtg tatagctatt ttgccgttgc gctggcgcag gctggtttgc gggtgatcat
     4681 gccggatgcg cccgatcacg gtagccgttt tagtggcgac gaagcgcggc ggttaaatca
     4741 attctggcaa atcttgctac aaagtatgca ggaattcact actttacgtg cggcaatagc
     4801 cgaagaaaac tggctgcttg atgaccgtct ggcagtcggt ggcgcgtcga tgggcgcgat
     4861 gacggcactg ggaattaccg ctcgccaccc cacggtgaaa tgtaccgcca gcatgatggg
     4921 atcgggctat tttacatcgc tcgcccgttc actgtttcca ccgctgatac ctgaaacggc
     4981 agcacagcag aatgaattca ataacattat cgcgccgctg gcagagtggg aagcgacaaa
     5041 ccacctggaa caacttgggg acagacctct actgctgtgg catggcctcg acgatgacgt
     5101 tgtgcctgcc gacgaatcac tacgtttgca gcaggcctta agcgagacgg gacgggataa
     5161 actgctaacc tgttcatggc agccaggcgt gcgtcaccgc attacgcctg aggcgttaga
     5221 tgctgccgtg acatttttcc gccagcatct ttaaacacgc agaatgctga ccccttgcgc
     5281 ttccagttgt tgcaggattt ccgggttagc atttttgccg gtgataagca tatcgatttg
     5341 atcggcacgg ctaaaaagca ttcccgcgcg ttcgccaatc ttactgctat caaccagtac
     5401 caccagtttc cctaccacgc tcagcatctt ctgctctgcc attgctgtca gcatatcggt
     5461 tttatacaac ccttctgcgg tcagcccttt tccgctggta aacatccagt gcccggcata
     5521 gagactgttt tcgctgccct gcgggcttaa agtgatggac tgacttttgt tgtactgtcc
     5581 acccataatg atcacgctgt catgttcttg atcgatcagg taatttgcca gcggtagata
     5641 attagtgatg atttgcactg gcttgccaca catttcccgc ccaagcagaa acgcggtgga
     5701 gccgcagttg atgactacgc tttcgcccgg attaaccagc tgcgaggccg ctttagcaat
     5761 acgtactttt tcatcgtgat tctgcgcctg atgcagattc atcggcgtcc agcgcgggcg
     5821 ctgttgggta atagcttctg cgccattgcg cacctttttc agtttgccgc gttcgtcaag
     5881 tttattgata tcgcgtcgcg cagtggcagg cgaaattccc agacgctcaa cgactttctc
     5941 aacggtcaca aagcccaatt gtgcgagcat ttccaggagg atttgatgtc tttgtgcttc
     6001 agtcatgagc tattccgata aaatttgatt tttttagatg atatttgaaa tagctaggaa
     6061 atactacgcc ggatagcgcg aacttctcca cgctatccgg tataaacgac agattacagg
     6121 aatgacttga acggcagatc cggttcaata gtgaagcaat catcgaaacc gcgcggatag
     6181 tggtactcga agttgtcttt atccagcggc caggtaaatt tgccgccaac ctgccagata
     6241 aacggcttga agccatactt cagacgatct tttttcatct cccacagcac gcggatctct
     6301 tgcggatcgg cctggaagtt tgaccagatg tcgtggtgga acgggataac cactttcgca
     6361 ttcagggctt cacccatacg cagcatatcg gcgctggtca ttttgtcggt aataccgcgc
     6421 gggttctcac catacgatcc taacgccaca tcgatctgat gttcgttacc gtgcttcgca
     6481 taatagttag agtagtggga atcgccactg tgatataggg aaccgccagg ggttttgaac
     6541 aggtagttta ctgcgcgatc gtccatgcca tctggcagta cgccagccgc tttttgatcg
     6601 gcaggcaggg tgatcagtgc agtacggtcg aaagcgtcaa gcgcatgaat ttcaatgtct
     6661 ttcactttta caacatcgcc cggtttgacc acgatgcaac gctctttcgg tacgccccag
     6721 ccaatccaca aatcaacgca ggttttcggt ccaatgaatg gtacgtcatc agcacagttc
     6781 tgcatcacgg cagcagcgac gttaacgtcg atatgatcgt tgtgatcgtg agtcgccagt
     6841 accgcgtcga tctggcgaat cgcaaacgga tcaagaacaa acggggtggt gcgtaggttt
     6901 ggctgcagtt ttttcacgcc agccatgcgc tgcatctggt gaccctgttt cattaacggg
     6961 ttaccgtgac tttgtttgcc agtgccgcac cagaaatcaa cgcaaacgtt ggtgccacct
     7021 tctgatttca accagatccc ggtgcagcct agccaccaca tcgcaaatgt gccaggggcg
     7081 acctgctctt gttcaatttc ttcattcagc cagctacccc actccgggaa agtgctcagg
     7141 atccaggatt ctcgggtgat acttttcact ttactcatcg ccatttacct tcatgatagt
     7201 tcaattcgaa tcaatatgtg attggttttg attaatcctg acactatttt ttcaggaagg
     7261 caatgaccat tttttgactt tttgccaggg aagttgttgt tgattttgag tgtggaaaga
     7321 tttaatggaa tgtgtagtta aattaactaa atgaatttaa ataaataatt gtttcgttgt
     7381 gtgaatccca ctctatccgt gtggaattat ttgcgggtcg cgtcacattt aatcataaat
     7441 aatcttgttg tgattacttt tgaaaattag agtgagtgca caacattccg ggtgtgcgga
     7501 atacccggtt acctcttctt caggagatcg ttatggagat cctctacaac atctttaccg
     7561 tgttttttaa ccaggtcatg accaatgccc cgttgttgct gggtattgtg acctgtctgg
     7621 gctacatcct actgcgcaaa agtgtcagcg ttattattaa aggcacgatt aaaaccataa
     7681 ttggtttcat gttgttgcag gcagggtccg gcatcctcac cagcaccttc aaaccggtgg
     7741 tggcgaaaat gtccgaagtc tacggcatta aacggcgcaa tttccgatac ttacgcgtcg
     7801 atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta cgcggtgttg
     7861 ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg tattcgtacc
     7921 atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt tacgttgttt
     7981 attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt ttcgctctac
     8041 tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga cggctgcggt
     8101 ttctccatcg gtcaccagca acagtttgca tcatggactg cctataaagt tgcgccgttc
     8161 ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct gaatattttc
     8221 cacgacaaca tcgtctccac ggcgattgtc atgaccatct tctttggtgc cattctgctc
     8281 tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgcactggac ggtctatatc
     8341 ctgcaaaccg gtttttcctt cgcggtggcg atcttcatca tcactcaggg tgtgcgcatg
     8401 tttgtggcgg aactctctga agcgtttaac ggtatctccc agcgcctgat ccctggtgcg
     8461 gttctggcga ttgactgtgc ggctatttat agcttcgcgc cgaacgccgt ggtctggggc
     8521 tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc ctgcggttcc
     8581 tcgatcctga ttatcccagg ctttatcccg atgttcttct ccaacgccac catcggcgtg
     8641 ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tttgtctggt gatggggatg
     8701 atcgaaatct ttggttgcgt ctgggcggtg aaacttaccg gtatgagtgc ctggatgggg
     8761 atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc catcggtatc
     8821 gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc tggccgcgca
     8881 ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta ataaggagtt
     8941 ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag ttccatgatc
     9001 atgaggatga aagtggacca gtttttaacc caatcaaaca ttgaccacac ggtaaatagc
     9061 tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat cgcttctacg
     9121 cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt gcgcaacatg
     9181 ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca tttcccgcag
     9241 gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa taaatccatc
     9301 cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt tgacctgctg
     9361 gtggcggatg ttgtcgagcc gcgttactac caggcgattc tggatgcggt tgaacagcac
     9421 ggtccgtact ttgtgctcgc accaggcctg gcaatgccgc acggtcgccc ggaagagggc
     9481 gttaagaaaa ccggttttgc gctggtgacg ctgaaaaagc cgctggagtt caaccacgaa
     9541 gataacgacc cggtggatat cctcatcacc atggcggcgg tcgatgccaa cacgcaccag
     9601 gaagtgggca tcatgcagat cgtcaacctg tttgaagatg aagaaaattt cgaccgttta
     9661 cgtgcctgcc gtaccgagca ggaagtactg gatctcattg accgcaccaa cgcggcagct
     9721 taagaaggaa ttgaagatgt cattaccgat gttgcaagtc gcgctggaca accagactat
     9781 ggatagcgcc tacgaaacca ctcgcctgat tgccgaagaa gtcgacatta tcgaagtggg
     9841 taccattctg tgcgtgggcg aaggcgtgcg tgcggttcgc gatctgaaag cgctctaccc
     9901 gcacaaaatc gtgctggcag acgccaaaat tgccgatgcg ggcaaaattc tttctcgtat
     9961 gtgcttcgaa gccaacgctg actgggtgac cgtgatttgt tgtgcggata tcaacaccgc
    10021 caaaggtgcg ctggacgtag caaaagagtt taacggcgac gtgcagatcg aactgaccgg
    10081 ttactggacc tgggaacagg cgcaacagtg gcgcgacgca ggtattcagc aggtggttta
    10141 tcaccgcagc cgtgacgcgc aggccgctgg cgtggggtga agccgatatc actgcgatca
    10201 aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg tggcctggcg ctggaagatc
    10261 tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc cggtcgcagc atccgtgatg
    10321 ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc catcgccgaa ctgtggggct
    10381 aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat ctatgaaaaa gcgctccccg
    10441 ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac gttgggcttc gattttgtcg
    10501 agatgtcggt agatgaaact gacgagcgcc tgtcgcgcct cgactggagc cgcgagcagc
    10561 gtctggcgct ggtgaatgcg attgttgaaa ccggcgtgcg tgtgccgtcc atgtgccttt
    10621 ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc ggtgcgggcg caggggctgg
    10681 agattatgcg taaagccatc cagttcgccc aggacgttgg cattcgcgtg atccagctgg
    10741 cgggctatga cgtttactat caggaagcca ataacgaaac gcgtcgtcgt ttccgtgacg
    10801 gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt gacgctggcg atggagatca
    10861 tggattatcc gttgatgaac tccatcagca aggcgctggg ttacgcgcac tatctcaaca
    10921 acccgtggtt ccagctctac ccggatatcg gcaacctgtc ggcgtgggac aacgatgtgc
    10981 agatggagtt gcaggccgga atcgggcata tcgtcgcggt gcatgtgaaa gacaccaaac
    11041 cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt ggatttcgaa cgttgcttcg
    11101 aaacgctcaa acagagtggc tattgcgggc cgtacctgat tgagatgtgg agcgaaacgg
    11161 cggaagaccc ggcggcagaa gtggtgaaag cgcgtgattg ggtgaaagcg cgcatggcca
    11221 aagcaggcac ggtggaggcg gcataatgca aaagctaaaa cagcaggtgt ttgaagccaa
    11281 catggatctg ccgcgctatg ggctggtgac ctttacctgg ggcaacgtca gcgctatcga
    11341 ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt gcctacgaaa ccatgaaagc
    11401 agacgatatg gtggtggtcg atatgagcgg caaggtggtg gaaggggagt atcgcccgtc
    11461 ttccgacacc gcgacgcatc tcgaactcta ccgtcgttac ccgtcgcttg gcggcattgt
    11521 ccatacccac tccactcatg ctactgcatg ggcgcaggcg gggctggcga tcccggcgtt
    11581 aggcactacg cacgccgact acttctttgg cgacatcccg tgtacgcgcg gattaagcga
    11641 agaagaggtg cagggcgagt atgaactgaa caccggcaaa gtgattatcg aaacgctggg
    11701 tgacgccgag ccgctgcata cgccggggat tgtggtgtat cagcacggtc cgttcgcctg
    11761 ggggaaagat gcccacgacg cggtgcataa cgcggtggtg atggaagaag tggcgaaaat
    11821 ggcgtggatt gcgcgcagta ttaacccaca actgaatcac atcgacagct tcctcatgaa
    11881 taaacacttc atgcgtaagc acggtcctaa cgcctattac gggcagaagt aagcagtgcg
    11941 ccccggaatt attacctcct gcgggatgta attggtctga ttccggggcc gcttcagtta
    12001 aaaaatcgta taaacaacag gtgtttttat ggctatcaat ggattgtttg tcagaacgga
    12061 tccgcaacgt cgccgctatg gcgttgcgtt acttgtgggt gtactttcag gaattatttc
    12121 agcatttgtt aaatggggag cggaagtccc cttgccaccg cgcacatttt ccggtggtcg
    12181 cgatgaattt aacccgccgt atatctttct acgggattat ttaggtatcg acccaaccca
    12241 aactgtgtat acattctcgg aacatattat caacccggtg atggtgacgc atatcatctt
    12301 ctcactggtt tttgcgatag ggtattgtat tgtcgcggaa attttcccga aagtgaaatt
    12361 atggcagggg atattagcag ggcttattgt gacagtggca gtccacggta tattttgccc
    12421 ggcgctgaat ttaacgccgc cgctcactca attgcctttt gacgaatatg cctctgaaat
    12481 cctggggcat attttctggt tctggataat agaaattatg cgccgcgact tacgtaatcg
    12541 cattactcat gagccagatc cggaagtcgc aatccggtga ataaccgcat aaaaaagccc
    12601 cctgcgaagg gggcaaagca aactatggca atgtttcgtt ggttatacct ggtgctagcg
    12661 ataaatatcc gcgctggcgt gcatattgcc gttactcccc ggttcccgca tcagaattac
    12721 gtggtagtac gttgcgccct gcgcatctgt ctcttcattt aatgcctgac gcgcttcgct
    12781 ttcagtggcg aaattatgat tgatataaat cacgcctaag ctttgcacat catccatgtt
    12841 tctggcctgg tggttattaa tttcaatggc tgcccatgta tttgcactta gcaaaagcac
    12901 agccagaagg gctaaaactc gactgaacat agatacctcc tcgacggctg actttgtgtg
    12961 ctctcctctg tgatgatctt ctgatttaat tttaatcaat gataaagaag ttgatggtga
    13021 ccatttctga tgcagttgtt caaaaaaaca ccatgatgaa gtgtgatgaa ctccaaatca
    13081 gcggtttaga ggttaattgc gaaaggggag atttatttcg gctctgccct tgagtttagc
    13141 ggggcataca agtactataa cgcgtcattt ttcagccgac ctttaacacg ttccttgcct
    13201 ccccgggatt cggctgaccc agacaggagg ctgaataatc cgtaaggagc aattcgatgc
    13261 gtcattacga aatcgttttt atggtccatc ctgaccagag cgaacaggtt ccgggcatga
    13321 tcgagcgcta cactgctgcc atcactggtg cagaaggcaa gatccaccgt ctggaagact
    13381 ggggccgccg tcagctggct tacccgatca acaaactgca caaagcacac tacgttctga
    13441 tgaacgttga agctccgcag gaagtgatcg atgagctgga aactaccttc cgcttcaacg
    13501 atgccgttat ccgcagcatg gttatgcgta ccaagcacgc tgttaccgaa gcatctccga
    13561 tggttaaagc gaaagacgag cgccgtgagc gtcgcgatga tttcgcaaac gaaaccgctg
    13621 atgatgctga agctggggat tctgaagagt aatttctgat gaccaaccgt ctggtgttgt
    13681 ccggcaccgt gtgcaggact ccccttcgaa aggtcagtcc atcaggaatt cctcactgcc
    13741 agttcgtgct tgagcatcgt tctgtgcagg aggaagccgg ttttcaccgg caggcgtggt
    13801 gccaaatgcc cgttattgtt agcggacacg aaaaccaggc cattactcac agtataacgg
    13861 tcggcagtcg cataaccgtt caggggttca tttcatgcca caaggcaaag aacggactga
    13921 gcaaaatggt tttgcatgcc gagcagattg aattgataga ttctggagac tagccatatg
    13981 gcacgttatt tccgtcgtcg caagttctgc cgtttcaccg cggaaggcgt tcaagagatc
    14041 gactataaag atatcgctac gctgaaaaac tacatcaccg aaagcggtaa gattgtccca
    14101 agccgtatca ccggtacccg tgcaaaatac cagcgtcagc tggctcgcgc tatcaaacgc
    14161 gctcgctacc tgtccctgct gccgtacact gatcgccatc agtaatcggt cacggtccat
    14221 taatacgact ttgagaggat aaggtaatgc aagttattct gcttgataaa gtagcaaacc
    14281 tgggtagcct gggtgatcag gtaaacgtta aagcgggcta tgctcgtaac ttcctggtac
    14341 cgcagggtaa agctgttcca gctaccaaga aaaacattga attcttcgaa gcacgtcgcg
    14401 ctgaactgga agctaaactg gctgaagttc tggcagctgc taatgctcgc gctgagaaaa
    14461 tcaatgcact ggaaactgtt accatcgcgt ctaaagctgg cgacgaaggt aaactgttcg
    14521 gttccatcgg tactcgcgac atcgctgacg ctgtaactgc agctggcgtt gaagtggcta
    14581 agagcgaagt tcgtctgccg aacggcgttc tgcgtaccac tggcgagcac gaagtgagct
    14641 tccaggttca cagcgaagta ttcgcgaaag tgatcgtaaa cgtagtagct gaataattcg
    14701 ttattcaacg agacgtaaaa acgccgacca gtggtcggcg ttttgctttt gtagcgagca
    14761 cttgcagata tcgataaggg ccacaataag cagccctacg aagataatta cttagctgcc
    14821 tgaatatcag ccagcattgg ggcaagggct gggttgtcct tagcgaagtt tgcgtacatt
    14881 ggctgtactt tctcgataaa cggagcttta tctacctggt agactttcac gcccatttta
    14941 tccagttttg caaattcttg tttctcttcc tcgctccaca agtctttcat gtagctcatt
    15001 gattctttag ctgcttttaa tataactgcc tgattttccg ggctaagttt atcaaacgca
    15061 gcattgctca tcagaaccac atcgggaacc atagtgtgtt cgtcaaggct gaaggatttt
    15121 gccacttcac cgtgacgcat ggtggtcagc gccatcacgc tgttttctgc catatcgacc
    15181 acaccctgct gtaaggctgt atacaactcg ccgtaatcca gtggcgtcgg gttaccgccc
    15241 atgactttga tcatttcaac tgcgccaggg cttggctgga cacggacttt catgcctttc
    15301 agatcgtctg gtttcagtac tggtttattg ccatagaaac tgcgggcgcc tccatcataa
    15361 aaagtcagcc cgaaataacc tttgcttttc gttgagtcga ggatttttct cccgatatcg
    15421 ccctgcatga cctgataata atgatcacga tcgcggaaca aataaggcag gctaaataat
    15481 ttataggaat tttcaaatgc ttccatttct gccgcattgg tttttaccag tggaatagcg
    15541 ccagaacgaa tcagctccat tgtttctcgt tgagtaccca atgttccatt tggataaata
    15601 cgaatagtaa tatcaccgtt agagtactct ttgttctttt tcgcaaagaa ctccatcgct
    15661 ttatgaacag gatgagactt atcctgatta tgactcagtt ttaatgtcac tgcccaggac
    15721 tggcttgcgg ataacataag aacagcaccg ataatgccag cggatacttt tattttcata
    15781 gatgttcctt ttctaataaa tatggcggca ggtgccgcca gtgaatgcgc atcagtagcc
    15841 cagtgctttt ggcaatgcca tactgatttg cggaatatag gtcaccattg ccagcaccaa
    15901 taccatcacg gcatagaatg gcagcatagg tttgataatt ttattaatcg gtaatttaga
    15961 tacactacaa ccgacaaata acacactgcc tacaggcgga gtacaaatac cgatacacat
    16021 gttcagtacc atgacaatac caaagtgaat cgggtccatg cccagttcgg tgacaacagg
    16081 taaaaatatc ggcgtaaaaa tcagtaccgc cggtgtcata tccataaaag tgccgataat
    16141 caacaggata atattgataa taagcaggat tattattggg ttgtcagaaa tagtgttaag
    16201 caaatcgaga atcaggaagg gaacgtcagc gtttgacatt gccca
//
LOCUS       DAZPEP010000088        16122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZPEP010000088 DAZPEP010000000
VERSION     DAZPEP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.088"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHUT9_002712"
     CDS             complement(71..1099)
                     /gene="malI"
                     /locus_tag="ACHUT9_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFR1349148.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1274..2866
                     /gene="malX"
                     /locus_tag="ACHUT9_002713"
     CDS             1274..2866
                     /gene="malX"
                     /locus_tag="ACHUT9_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFR1349149.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2876..4048
                     /gene="malY"
                     /locus_tag="ACHUT9_002714"
     CDS             2876..4048
                     /gene="malY"
                     /locus_tag="ACHUT9_002714"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFR1349150.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4152..5153
                     /gene="add"
                     /locus_tag="ACHUT9_002715"
     CDS             4152..5153
                     /gene="add"
                     /locus_tag="ACHUT9_002715"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFR1349151.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5189..6229)
                     /locus_tag="ACHUT9_002716"
                     /pseudo
     CDS             complement(5189..6229)
                     /locus_tag="ACHUT9_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            6473..6598
                     /gene="blr"
                     /locus_tag="ACHUT9_002717"
     CDS             6473..6598
                     /gene="blr"
                     /locus_tag="ACHUT9_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFR1349152.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHUT9_002718"
     CDS             6863..7078
                     /gene="ydgT"
                     /locus_tag="ACHUT9_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFR1349153.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7164..7604
                     /locus_tag="ACHUT9_002719"
     CDS             7164..7604
                     /locus_tag="ACHUT9_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFR1349154.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHUT9_002720"
     CDS             7681..8262
                     /gene="rsxA"
                     /locus_tag="ACHUT9_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFR1349155.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHUT9_002721"
     CDS             8262..8840
                     /gene="rsxB"
                     /locus_tag="ACHUT9_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFR1349156.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8833..10767
                     /gene="rsxC"
                     /locus_tag="ACHUT9_002722"
     CDS             8833..10767
                     /gene="rsxC"
                     /locus_tag="ACHUT9_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFR1349157.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVA
                     AAIARAQAKKAAQQKVVNED"
     gene            10768..11826
                     /gene="rsxD"
                     /locus_tag="ACHUT9_002723"
     CDS             10768..11826
                     /gene="rsxD"
                     /locus_tag="ACHUT9_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFR1349158.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            11830..12450
                     /gene="rsxG"
                     /locus_tag="ACHUT9_002724"
     CDS             11830..12450
                     /gene="rsxG"
                     /locus_tag="ACHUT9_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFR1349159.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            12454..13149
                     /gene="rsxE"
                     /locus_tag="ACHUT9_002725"
     CDS             12454..13149
                     /gene="rsxE"
                     /locus_tag="ACHUT9_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFR1349160.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            13149..13784
                     /gene="nth"
                     /locus_tag="ACHUT9_002726"
     CDS             13149..13784
                     /gene="nth"
                     /locus_tag="ACHUT9_002726"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFR1349161.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            14396..15898
                     /gene="dtpA"
                     /locus_tag="ACHUT9_002727"
     CDS             14396..15898
                     /gene="dtpA"
                     /locus_tag="ACHUT9_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFR1349162.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            16003..>16122
                     /locus_tag="ACHUT9_002728"
     CDS             16003..>16122
                     /locus_tag="ACHUT9_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFR1349163.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENG"
BASE COUNT         3737 a         4104 c         4116 g         4165 t
ORIGIN      
        1 ctgtgtgcca cattttttat cattaacctt gaggaaagga cactaccagg tatagcagat
       61 aaagagagga ttatttcgct gcaatgagcc gggcgggaat aataagattg cgtgaatgcg
      121 tctcttcatg ggtgattttt tgcatcatgc gatccgcaag tgtggtacca agttcccgcg
      181 caggcgtgct ggcccaggta acgggtatat catcaagtgt ggttggtgtc gcatcggtaa
      241 atgccgccag cgaaacttgt tgctcaaagt aacgatcgac tccgctttcc ccgctttgcc
      301 gccctgcttt cagcaaacca aaccatgccc ccatcgcaat agtttcgtta tagcaaacca
      361 cagcactgat ggtcgggtta tgacgtaaaa gcgccgtgat agcttccgcg gcttgcttct
      421 ggctggatgt gcactccagc acccaatcgc tgtgaaacgg caggccaaat tttagtagag
      481 ttgcacaata gcctcccacc cgttctgccc gggttaatga ggaactttgc cctcccagcc
      541 aggcgatccg ctgatgccca ttgcgaatga gatgctccgt caacaactgt gcagcctgca
      601 tgttgtccgg gcgaaccgta tcaacatcat caagataact ggcacgggaa gcgaaaatca
      661 ccgggatagc tttttcttct gccatccgtc gcaggtcatc gctgcttcct gcagccccgg
      721 caattaccac accatcgaca ccctgattca gtaacagtga aaaccgctgt gccagctgct
      781 cgccgtcttt accgccgtga agcaaaaaaa ccatccgtcc ctgcgcttcc agagcttccg
      841 tcaatccggc cgtcaattcg gcgtaaaacg gcgcagataa atcacggacg atcaaaccaa
      901 tgacgccgct ttgcccgccg cgcagcgccg acgcctggcg attgcgcaca aatcccagct
      961 cttcaatggc ggcgttaacg cgttctcctg tggcggtaga gattcgccct ttgccactaa
     1021 gcaccagcga aacggtactt accgacacgc ccgcagccag cgcaacatca tgaatggtta
     1081 tttttttggc ggtagccatg cagatgacct actccctgat tatgtgacag ataaaacgtt
     1141 ttacctttta tttatcttat acccgctatt atcgttgcgt aatgtgattt atgcttcact
     1201 aaaatttgat aaaacgtttt atcttctcgc gcaatttact gaatccagat tgttctctac
     1261 gaggagtcgt tttatgacgg cgaaaacagc accgaaagtc acgctgtggg agttcttcca
     1321 gcagttaggc aaaaccttca tgttacccgt ggcattattg tcgttctgcg gcattatgct
     1381 cggcattggt agttctctta gtagccacga tgtcatcacc ctgatcccgg tcctgggcaa
     1441 ccccgtgttg caggctatct ttacctggat gagtaagatt ggctcgtttg cttttagttt
     1501 cctgccagtc atgttctgta tcgccatccc gctgggtctg gcacgcgaaa acaaaggcgt
     1561 agcggcattc gctggcttcg tcggttatgc ggtaatgaac ctcgcggtaa acttctggtt
     1621 gaccaataaa ggcattctgc caaccacgga cgccgcggtt ctgaaagcca ataacatcca
     1681 gagcattctt gggatccagt cgatcgacac cgggatcctc ggtgcggtga tcgccggtat
     1741 tatcgtctgg atgctgcatg agcgattcca taatatccgc ctgccggatg cgcttgcatt
     1801 cttcggcggt acgcgcttcg taccaatcat ctcctcgctg gtgatgggcc ttgtcggcct
     1861 ggtgattcca ttagtctggc cgattttcgc gatgggtatt agcggcttgg gccatatgat
     1921 aaacagcgcg ggtgatttcg gaccgatgct gtttggtacc ggtgaacgtc tgctgttgcc
     1981 gtttggtctg catcacattc tggtggcatt aattcgcttt accgacgcag gcggcacgca
     2041 ggaagtctgc ggtcaaaccg tcagcggcgc attgaccatc ttccaggcgc aattgagttg
     2101 cccgaccact cacggttttt ctgaaagcgc cacgcgtttc ctttcgcaag gcaaaatgcc
     2161 tgcgtttctc ggcggtctgc caggtgcagc gttagcgatg tatcactgcg cgcgcccgga
     2221 aaatcgccat aaaattaaag ggctgctgat ttctggcctg atcgcctgtg tcgttggcgg
     2281 cacgaccgaa ccgctggaat tcctgttcct gttcgtggcg ccagttctgt atgtcatcca
     2341 cgcgctgtta accggcctcg gcttcactgt catgtctgtg ctcggcgtca ccatcggtaa
     2401 taccgacggc aatatcatcg acttcgtggt gttcggtatt ttgcatggtc tgtcaaccaa
     2461 gtggtacatg gtgccagtgg tggcggcaat ctggtttgtc gtttactacg tcatcttccg
     2521 tttcgctatc acccgcttca atctgaaaac cccggggcgc gatagcgaag tggccagttc
     2581 aatcgaaaaa gccgttgccg gtgcgccggg taaatcaggt tacaacgttc cggcaatcct
     2641 cgaagcctta ggcggtgccg acaatattgt tagcctcgat aactgcatta cccgtctgcg
     2701 tttgtctgtg aaagatatgt cgcttgttaa tgtgcaggca ctgaaggaca atcgggcaat
     2761 tggcgtagta caacttaatc aacataacct gcaggttgtt atcgggccac aagttcagtc
     2821 agtaaaagat gaaatggccg gtctgatgca tactgtccag gcataaggat aagatatgtt
     2881 cgatttttca aaggtcgtgg atcgtcatgg cacatggtgt acacagtggg attatgtcgc
     2941 tgaccgtttc ggcactgctg acctgttacc gttcacgatt tcagacatgg attttgccac
     3001 tgccccctgc attatcgagg cgctgaatca gcgcctgatg cacggcgtat ttggctacag
     3061 ccgctggaaa aacgatgagt ttctcgcggc tattgcccac tggttttcca cccagcatta
     3121 caccgcaatt gatacgcaga cggtggtgta tggcccttct gtcatctata tggtttcaga
     3181 actgattcgt cagtggtctg aaacaggtga aggcgtggtg atccacacgc ccgcctatga
     3241 cgcattttac aaggccattg aaggtaacca gcgcacagta atgcccgttg ctttagagaa
     3301 gcaggctgac ggctggtttt gcgatatggg caagctggaa gccgttttgg cgaaaccaga
     3361 atgtaaaatt atgctcctgt gtagcccaca gaatcctacc gggaaagtgt ggacgtgcga
     3421 tgagctggag atcatggctg acctgtgcga gcgtcatgat gtgcgggtta tttccgatga
     3481 aatccatatg gatatggtct ggggcgagca gccgcatatt ccctggagta atgtggcgcg
     3541 gggagactgg gcgttgctga cgtcgggatc gaaaagtttc aatattcccg cgctgaccgg
     3601 tgcttacggg attatagaaa atagcagcag ccgtgatgcc tatttatcgg cactgaaagg
     3661 acgtgatggg ctttcttccc cttcggtact ggcgttaact gcccatatcg ccgcctatca
     3721 gcaaggtgca ccgtggctgg atgccttacg cgtctatctg aaagataacc tgacgtatat
     3781 ctcagataaa atgaacgccg cgtttcctga actcaactgg cagatcccgc aatccactta
     3841 tctggcctgg cttgatctac gtccgttgaa tattgacgac aacgcgttgc aaaaagcgct
     3901 tatcgagcaa gaaaaagtcg cgatcatgcc ggggtatacc tatggtgaag aaggtcgtgg
     3961 ttttgtccgt ctcaatgccg gctgcccacg ttcgaaactg gaaaaaggtg tggctggatt
     4021 aataaacgcc atccgcgctg ttcgctaacc ccaattgcgc aacgtaaaaa atcgttgcgc
     4081 aatcgtggat ttttaccctg ctttgttttt ataatggtgc gcacttttat atccagaaaa
     4141 agagtgcgac catgattgat accaccctgc cattaactga tatccatcgc caccttgatg
     4201 gcaacattcg tccccagacc attcttgaac ttggccgcca gtataatatc tcgcttcctg
     4261 cacaatccct ggaaacactg attccccacg ttcaggtcat tgccaacgaa cccgatctgg
     4321 tgagctttct gaccaaactt gactggggcg ttaaagttct cgcctctctt gatgcctgcc
     4381 gccgcgtggc atttgaaaac attgaagatg cagcccgtaa cggcctgcac tatgtcgagc
     4441 tgcgtttttc accaggctac atggcaatgg cacatcagct gcctgtagcg ggtgttgtcg
     4501 aagcggtgat cgatggcgta cgtgaaggtt gccgcacctt tggtgtgcag gcgaagctta
     4561 tcggcattat gagccgaacc ttcggcgaag ccgcctgtca gcaagagctg gaggcctttt
     4621 tagcccaccg tgaccagatt accgcacttg atttagctgg tgatgaactt ggattcccgg
     4681 gaagtctgtt cctttctcac ttcaaccgcg cgcgtgatgc gggctggcat attaccgtcc
     4741 atgcaggcga agctgccggg ccggaaagca tctggcaggc gattcgtgaa ctgggtgcgg
     4801 agcgtattgg acatggcgta aaagccattg aagatcgggc gctgatggat tttctcgccg
     4861 agcaacaaat tggtattgaa tcctgtctga cctccaatat tcagaccagc accgtagcag
     4921 agctggctgc acatccgctg aaaacgttcc ttgagcatgg cattcgtgcc agcattaaca
     4981 ctgacgatcc cggcgtacag ggagtggata tcattcacga atataccgtt gccgcgccag
     5041 ctgctgggtt atcccgtgag caaatccgcc aggcacagat taatggtctg gaaatggctt
     5101 tcctcagcgc agaggaaaaa cgcgcactgc gagaaaaagt cgccgcgaag taacaaaaat
     5161 ggatggtgca atttgcacca tccatttttc acgcaaggca caacgtcgcg cgatgtttgg
     5221 cggattcgat gcccagctca atcaactcca ttacctggat tgcctagctt gccggaaccg
     5281 gattttcacc atcgccattt aacgcatcac gaatcgccgc atagtaagcc ggataattcc
     5341 caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg gtaagtacgc
     5401 catcacgcat atcgtagccc cagtcttcct gcggcagacg ctcgccattt ttcagacgtt
     5461 cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc acgatatacc
     5521 gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc tgtggatagg
     5581 acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt gccaaatcta
     5641 ccgtcatgct gaccggtaaa ccaaatagcg taatggcctg atcaagaaga tgcggtgcta
     5701 aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg cgcacctgcg
     5761 gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg ccttccgcga
     5821 gcaaaccttt tagcgtcaag aaatcgctat cccaacgacg gttatggaat acagacagca
     5881 cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt gtcacggtaa
     5941 agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct aacgggaaat
     6001 gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta aacagatgct
     6061 tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt tcatcactgc
     6121 tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca tggaaggttt
     6181 tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg tcattgcctc
     6241 tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca gtttaataac
     6301 ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aaatagcgaa ttttccaacg
     6361 acaaaagcta aatatcgcaa aaacctcagt aaaaatcttg ctggagctat tattgctaag
     6421 taacatttac cccctgaagt taatggatca atcaagagag atgtgggctg taatgaatcg
     6481 tcttattgaa ttaacaggtt ggatcgttct tgtcgtttca gtcattcttc ttggcgtggc
     6541 gagtcacatt gacaactatc agccacctga acagagtgct tcggtacaac acaagtaagc
     6601 tctgcacttg tggagcgaca tgctgcccgt ccgggtgcat gttttcactt gtcggatatt
     6661 aaaccaggaa tttattatct tgttcgatgt tgttggtgat tgtcagggat agtaaagcgc
     6721 gttactcttt tcagagcagt ttttcatccc tgaatatttg ccatcagcgc attcttatta
     6781 ttgtaataac acaagcgttc acttttggtg aaattttatc atcactctac ccctttggta
     6841 ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat ttcgcaaaat
     6901 cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc tgacggacga
     6961 tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc tggtttctgg
     7021 cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg tccaataaag
     7081 aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga tttgcctgtt
     7141 tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt ggaggctggt
     7201 tgcttggccc gttggcttgg cttttagtcg ctctgttgag tacgacgctg gcgttgttgc
     7261 tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga caagcactga
     7321 cgacacaaat cttatggggc gtttctttta ttaccgctat cgccatgtgg tattacacac
     7381 tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac tatattatct
     7441 ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc gtggaagatg
     7501 gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc gtcccctatt
     7561 tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct tacagttaac
     7621 ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc aggccgaaaa
     7681 atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt tgtactggtc
     7741 aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac cgcgatgggc
     7801 atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg gcttatcgat
     7861 acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt tattctggtg
     7921 attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc ggtgctttac
     7981 cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct cggcgtggcg
     8041 ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg tttttccgcc
     8101 gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg ccttgctgtg
     8161 gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc aggtcttatg
     8221 tctctggcct ttatgggctt taatggtttg gtgaagttgt aatgaatgct atctggattg
     8281 ccgttgccgc cgtgagcctg ctgggcttgg cgtttggcgc cattctgggt tatgcctccc
     8341 gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc ttaccgcaga
     8401 gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc atcagctgta
     8461 acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta aaaattgccg
     8521 agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta acgcctgcgc
     8581 ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt attcaggctt
     8641 gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg agtgatctct
     8701 gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg ttgcaaccgg
     8761 tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg cgtatcattc
     8821 ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat ctgggatttc
     8881 aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc cctgcgccag
     8941 gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc tgaaggtgag
     9001 ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg tggtcgcggc
     9061 aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc gccccactct
     9121 acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga tggtgaagac
     9181 tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga agagttaatc
     9241 gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc gacaggcgtt
     9301 aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc tgagtgcgag
     9361 ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt cgtagagggt
     9421 attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat tgaagataac
     9481 aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga tatttctctg
     9541 cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta cattctgacc
     9601 gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat gcaaaacgtc
     9661 ggcactgctt atgcagtgaa acgtgccgtt attgatggcg agccgattac cgagcgtgtt
     9721 gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg gctggggacg
     9781 ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca aatggtgatt
     9841 atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt cgtaaagatt
     9901 accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga agaacaaagc
     9961 tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc gcaacagttg
    10021 tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat tgctgattgc
    10081 attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt gcaatatttc
    10141 cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc cgcagaagcc
    10201 aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc tcgccttgaa
    10261 cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat tgctgccgct
    10321 ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat taaagcgggc
    10381 gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc gcaagccaga
    10441 gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga tccacgtaaa
    10501 actgccgttg aagcagctat cgcccgcgcc aaagcgcgca agctggaaca gcaacaggct
    10561 aatgcggaac cagaagaaca ggtcgatccg cgcaaagccg ccgtcgaagc cgctattgcc
    10621 cgtgccaaag cgcgcaagct ggaacagcaa cagactaacg cagaaccaga agaacaggtt
    10681 gatccgcgca aagcggcagt tgccgcggct attgcccgcg ctcaggccaa aaaagccgcc
    10741 cagcagaagg ttgtaaacga ggactaaatg gtattcagaa tagctagctc cccttatacc
    10801 cataaccagc gccagacatc gcgcattatg ctgttggtgt tgctcgcagc cgtgccagga
    10861 atcgcagcgc aactgtggtt ttttggttgg ggtactctcg ttcagatcct gttggcatcg
    10921 gttagtactc tgttagccga agctctcgta ctcaaactac gcaagcagtc ggtagccgca
    10981 acgttgaaag ataactcagc attgctgaca ggcttattgc tggcggtaag tattcccccc
    11041 ctcgcgccat ggtggatggt cgtgctgggt acggtgtttg cggtgatcat cgctaaacag
    11101 ttgtatggcg gtctggggca aaacccgttt aatccggcaa tgattggtta tgtggtctta
    11161 ctgatctcct tcccggtgca gatgaccagc tggttaccgc cacatgaaat tgcggtcaac
    11221 atccctggtt ttatcgacgc catacaggtt attttcagcg ggcataccgc cagtggtggt
    11281 gatatgaaca cactacgctt aggtattgat ggcattagtc aggcgacacc gctggataca
    11341 tttaaaacct ctgtccgtgc cggtcattcg gttgaacaga ttatgcaata tccgatctac
    11401 agcggtattc tggcgggcgc tggttggcaa tgggtaaatc tcgcctggct ggctggcggc
    11461 ctgtggttgc tatggcagaa agcgattcgc tggcatattc ccctcagctt cttagtaacg
    11521 ctggcgttat gcgcaacgtt gggctggttg ttctcaccag aaacactggc agcaccgcaa
    11581 attcatctgc tgtctggtgc gaccatgctc ggcgcattct ttattttgac tgacccggtt
    11641 accgcttcta cgaccaatcg tggtcgtctt attttcggcg cgcttgcggg cttattagtc
    11701 tggttgatcc gcagtttcgg cggctatcct gacggcgtgg cttttgccgt cctgctggcg
    11761 aacatcacgg ttcctctgat cgattactac acgcgtccgc gcgtctacgg ccatcgcaaa
    11821 gggtaaacca tgctgaaaac tatccgaaaa cacggcatta cgttagcgct atttgcagcg
    11881 ggttcaacag ggttaactgc ggccatcaac cagatgacca aaacgacgat tgctgaacag
    11941 gccagtctgc aacaaaaggc attatttgat caggtgctgc cagccgaacg ctataacaat
    12001 acgctggcac agagttgcta tctggtaact gcgccagagt taggtaaagg tgagcatcgg
    12061 gtttacatcg ccaaacagga tgacaaaccg gtagccgccg ttctggaagc aaccgcgcca
    12121 gatggctatt ccggtgcgat tcagctgctg gtgggagccg attttaacgg cacggtactt
    12181 ggcacgcgcg tgacagagca ccacgaaacg ccagggcttg gcgataaaat cgaactgcgc
    12241 ctttctgact ggatcaccca ttttgcgggt aaaaaaatca gtggtgcgga tgatgcgcac
    12301 tgggcggtga agaaagatgg tggtgatttc gaccagttca ccggcgcgac gattactccc
    12361 cgcgcggtgg ttaatgcggt aaaacgcgcc ggattgtacg ctcagacgtt accggcacaa
    12421 ctttctcaac ttcctgcctg tggagaataa agcgtgagcg aaattaaaga cgttattgtt
    12481 caggggttgt ggaaaaacaa ctctgcgctg gtccagttgc tcggcctttg tcctctgttg
    12541 gcggtcacgt ccactgccac taacgctctg ggtttaggac ttgcgactac gctggtactg
    12601 acgctgacca acctgaccat ttcgacgctg cgtcactgga cgccagccga gatccgcatt
    12661 cccatttacg tgatgatcat cgcctcggtg gtcagcgctg tacagatgct gatcaacgcc
    12721 tacgcctttg gcctgtatca atcattaggg atttttattc cgctgattgt cactaactgt
    12781 atcgttgtgg gccgcgctga agccttcgcc gccaaaaaag gtccggcgct ttcggcactg
    12841 gacggctttt caattggtat gggcgcaacc tgcgccatgt tcgtgctggg ttcactacgc
    12901 gaaattatcg gcaatggcac attgtttgac ggtgcagatg cgctgttagg tagctgggca
    12961 aaagtattac gcgtggagat tttccacacc gactcccctt tcctgctggc gatgctgcca
    13021 ccaggtgcat ttattggcct gggactgatg ctggcaggaa aatacctgat tgatgaaaga
    13081 atgaaaaagc gccgtactga agcagctgcc gaacgtgcat tgccaaacgg tgaaacaggg
    13141 aatgtctgat gaacaaagca aaacgcctgg agatcctcac tcgcctgcgt gagaacaatc
    13201 ctcatcccac caccgagctt aatttcagtt cgccttttga attgctgatt gccgtactgc
    13261 tttccgctca ggcgaccgat gtcagtgtta ataaggcgac ggcgaaactt tacccggtgg
    13321 cgaatacgcc tgcagcgatg cttgaactgg gcgttgaagg ggttaaaacc tatatcaaaa
    13381 cgattgggct ttataacagc aaagcagaaa atatcatcaa aacctgccgt atcttactgg
    13441 agcagcataa tggcgaggtt ccggaagatc gtgctgcgct tgaagccctg cccggcgtag
    13501 gtcgtaaaac agccaacgtc gtattaaaca ctgcattcgg ctggccgact attgctgtcg
    13561 acacgcacat tttccgcgtt tgtaatcgta ctcaatttgc gccggggaaa aacgtcgaac
    13621 aggtagaaga aaagctactg aaagtggttc cggcagagtt taaagtcgac tgccaccatt
    13681 ggttgatcct gcacgggcgt tatacctgca ttgcccgcaa gccccgctgt ggctcttgta
    13741 ttattgaaga tctttgtgaa tacaaagaga aagttgacat ctgaagaaaa ggggtaacac
    13801 cgattacccc attgataacc tttctttatc ctcttttaaa acatcttttt aacgtgaatg
    13861 atgccattgc ttagcgttat catcaggtaa tccgtttgcg gataaccaaa aatgcaggtt
    13921 aattgttttt ttaatagcga aattttctat tcattcgtga tcaagatcac gatgctgtcg
    13981 caatgtaaaa acattgcaag aatcaggtta aaataattta aatttacaca cgcaacaaat
    14041 attgacctac aaaacattac actggctatt tttcagaaac tggactatct cactagtaaa
    14101 aacgccaaat agcagaacat atcgccgtta agccatcacc ccccctctca gtgcagtgaa
    14161 aaaatctgcc attacgtttt ttgaaaaatt taacgctgga taacatttcc cggaatggtt
    14221 gaattccccg cctcagttat atgtaacaga ttattacaaa ggacttgtct gaaagtgcaa
    14281 gatagtgaac attacctgcc gtttcccctc ccactataac aattgcgcgt atgtttttta
    14341 gacataacgc gagaaagccc ccccgttaat atgggatgta aaaaaagagg taaaagtgtc
    14401 cactgcaaac caaaaaccaa ctgaaagcgt cagtttgaac gctttcaaac aaccgaaggc
    14461 gttctatctc atcttctcga ttgagttatg ggaacgtttt ggttattacg gcctacaagg
    14521 aattatggct gtttacctgg ttaaacaact gggtatgtct gaagcggatt caatcaccct
    14581 tttctcttcc tttagtgccc tggtttatgg tctggtcgct atcggcggct ggttaggtga
    14641 caaggtactg ggtactaaac gcgtaattat gctcggcgct attgtgctgg cgattggtta
    14701 tgctctggtt gcctggtctg gtcacgacgc cggtatcgtt tatatgggta tggcggctat
    14761 tgcggtcggt aacggcctgt ttaaagctaa cccatcttct ctgctttcta catgctatga
    14821 gaaaaacgac ccgcgtctgg acggtgcatt caccatgtac tacatgtccg tcaacatcgg
    14881 ctctttcttc tctatgattg ctacgccgtg gctggccgcg aaatacggct ggagtgttgc
    14941 gtttgcgttg agcgttgtag gcctgctgat cactatcgtt aacttcgcct tctgccaacg
    15001 ctgggttaaa cagtacggtt caaaaccaga cttcgagcct atcaactacc gtaacctgct
    15061 gctgaccatt attggtgttg tggcactgat cgctatcgcc acctggctgc tgcacaatca
    15121 ggaagttgcg cgtatggcgc tgggcgttgt tgccttcggt atcgtggtta tcttcggtaa
    15181 agaagccttc gcgatgaaag gtgctgcgcg tcgtaaaatg atcgttgcct tcatcctgat
    15241 gctcgaagcc attatcttct tcgtgctgta cagccagatg ccaacgtcac tgaacttctt
    15301 tgcgattcgt aacgttgagc actccattct gggtctggcc gtagaacctg agcagtatca
    15361 ggcactgaac ccgttctgga tcatcatcgg tagtccgatt ctggccgcta tctataacaa
    15421 gatgggcgat accctgccga tgccaaccaa gtttgcaatc ggcatggtga tgtgttctgg
    15481 tgcgttcctg attctgccgc tgggtgcgaa attcgcgtct gacgctggta tcgtgtctgt
    15541 aagctggctg gtcgcaagct atggcctgca gagcatcggg gaactgatga tctctggtct
    15601 gggtctggca atggttgcac aactcgttcc gcagcgtctg atgggcttca ttatgggtag
    15661 ctggttcctg accactgccg gtgcaaacct gattggtggt tatgttgcgg gtatgatggc
    15721 tgtgccggat aacgttaccg acccgctgat gtcactggaa gtttatggtc gcgtattctt
    15781 gcagattggt gtcgctactg ccattattgc agtactgatg ctgctgaccg cgcctaaact
    15841 gcaccgtatg acgcaggatg acgctgcaga caaagcggcg aaagctgctg tagcgtaaaa
    15901 ttcagggaaa ctcttttaca agccgctaac ttttcgttag cggctttttt ttgttcagca
    15961 acgcactacc atactttaaa ccacagacaa aaggagttac cgatgaaatt gttctacaaa
    16021 ccgggtgcct gctctctcgc ttcccatatc accctgcgtg agagcggaaa ggattttacc
    16081 ctcgtcagtg tggatttaat gaaaaaacgt ctcgaaaacg gt
//
LOCUS       DAZPEP010000089        16109 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZPEP010000089 DAZPEP010000000
VERSION     DAZPEP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.089"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..81)
                     /locus_tag="ACHUT9_002729"
     CDS             complement(<1..81)
                     /locus_tag="ACHUT9_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1349164.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTL"
     gene            complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHUT9_002730"
     CDS             complement(199..1638)
                     /gene="rsmF"
                     /locus_tag="ACHUT9_002730"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFR1349165.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHUT9_002731"
     CDS             complement(1719..4352)
                     /gene="yebT"
                     /locus_tag="ACHUT9_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFR1349166.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHUT9_002732"
     CDS             complement(4321..5604)
                     /gene="yebS"
                     /locus_tag="ACHUT9_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFR1349167.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5734..6231
                     /gene="msrC"
                     /locus_tag="ACHUT9_002733"
     CDS             5734..6231
                     /gene="msrC"
                     /locus_tag="ACHUT9_002733"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFR1349168.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6328..7026
                     /gene="proQ"
                     /locus_tag="ACHUT9_002734"
     CDS             6328..7026
                     /gene="proQ"
                     /locus_tag="ACHUT9_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFR1349169.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7046..9094
                     /gene="prc"
                     /locus_tag="ACHUT9_002735"
     CDS             7046..9094
                     /gene="prc"
                     /locus_tag="ACHUT9_002735"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFR1349170.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9286..10167
                     /gene="htpX"
                     /locus_tag="ACHUT9_002736"
     CDS             9286..10167
                     /gene="htpX"
                     /locus_tag="ACHUT9_002736"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFR1349171.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10213..11276)
                     /locus_tag="ACHUT9_002737"
                     /pseudo
     CDS             complement(10213..11276)
                     /locus_tag="ACHUT9_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHUT9_002738"
     CDS             11453..12244
                     /gene="kdgR"
                     /locus_tag="ACHUT9_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFR1349172.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12387..12626)
                     /locus_tag="ACHUT9_002739"
     CDS             complement(12387..12626)
                     /locus_tag="ACHUT9_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFR1349173.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHUT9_002740"
     CDS             12785..12928
                     /gene="mgrB"
                     /locus_tag="ACHUT9_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFR1349174.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13003..13290
                     /locus_tag="ACHUT9_002741"
     CDS             13003..13290
                     /locus_tag="ACHUT9_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFR1349175.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            13960..14103
                     /locus_tag="ACHUT9_002742"
     CDS             13960..14103
                     /locus_tag="ACHUT9_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFR1349176.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14116..14325
                     /gene="cspE"
                     /locus_tag="ACHUT9_002743"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14116..14325
                     /gene="cspE"
                     /locus_tag="ACHUT9_002743"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1349177.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHUT9_002744"
     CDS             14491..15300
                     /gene="rlmA"
                     /locus_tag="ACHUT9_002744"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFR1349178.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHUT9_002745"
     CDS             complement(15297..15863)
                     /gene="mntP"
                     /locus_tag="ACHUT9_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFR1349179.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4201 a         3929 c         4096 g         3883 t
ORIGIN      
        1 taacgttcga tcacccggcg attcaccgtg taattcgccg tcgtcgattt caaaagcgcc
       61 tatgcgcaca ctggttttca tcttcgtgct ctccatttag ctgccggttg tggtacgacc
      121 agtccgatcg caccattttt cagcctagtt gaggcagccg aatgtgccag tcaaaaagtg
      181 cgcttttttt gtgcgctgtc aggcgttacc ggtaaaaagt ttcccatcgc gcaccagttc
      241 acgcggatag ctgtttttca atcgcgaacc aatccgtttg gctaaaccaa tcggttgatg
      301 ctggaaagta accaacacgt catccgccac tggcgcggct tgcgggtaaa catcgcgccc
      361 gcgataccac tcctccgctt cctgcggtgt cagttcaaaa gcgttcacat tgtcgggggt
      421 ggcaagggca ataactgctt catgctgcca gcgataacct ttgttgtgcg tttcggcaag
      481 tttaatcccc aagcgagaaa atcggacttt accgatcagg gcttcaatgc ccaccgggaa
      541 caaccacagt tctttgtcac gctgccagag gcgcaggttt tcatcccagt ttaagccaac
      601 acctgcagcc gcctgacgaa tttgtccagc ttcgcgatct ttcactgggc tgaacggaaa
      661 attaccgact ttgtatttgg gggcgggtaa ggcgggaatc gcctgagttt tacgcagacg
      721 agcaacgaag aagccttcgc agtcgtaaat ttgtgggaaa acatgcaaaa agccttcctc
      781 ggtcagcgcc ttgtttgcac cagggaagag atcaccaagc ggtaaaaact ctactgcgtc
      841 ggggtaagtc tctttcagcc acaggcaaac ggcttcgttt tcttcctggt ttaaggtaca
      901 ggtcgagtaa accagcgtac cgccagggcg taatgcatga aaggcgctgt cgatcagttc
      961 ccgttgggta gctgcgattt cctgattgct ttctggtgac cagtttttta gcgcatcggg
     1021 atctttacgc accacgcctt cgccggagca gggagcgtcc agcaaaatgg catcaaacat
     1081 ttctggcact gccgcaccaa acacgcggcc atcaaaatgt gtgagcgcaa cattactgat
     1141 accacagcgg ctgatattgg catgtaacac ttttacccga ctggcggaaa actcattggc
     1201 aagaattgcc ccttcgttat tcatccgcgc ggaaatttgc gtcgttttgg agccaggcgc
     1261 ggcagcgaca tccatcaccc gctgtggtgc attaccgtca gcaaacaagg cggcgacggg
     1321 caacattgaa ctggcttcct gaatataaaa caggccactt aaatgctcgg cggtactacc
     1381 caatggcaat gcatcttcat tgtcgcgttc aatccagaaa ccttcttcac accacggaat
     1441 tggcgtaagc gtccagccat aaggagcggt taattgcagg aaatcagcaa cggagatttt
     1501 cagcgtatta acgcgaatgc tgcggcgcaa cgggcgctga caggcggcaa gaaaatcatc
     1561 aaaagagagc gtcgaaggca tcgcttcgcg catttgcgtc agaaaggcgt ccgggaaata
     1621 aacggtgtgt tgggccacga gcatatacca ctggcaaaaa aaacaggcgc gcagtttagc
     1681 ataaacgctc cggcgcaggc acgccggagc agtgggcatt atttgggaag cgcagtaccc
     1741 cattcacgcc actctttcgg ttcactttcc tgcaacagga agtgcttgcc ttcctgggct
     1801 ttcggtgcca gtggcgtacc cggaggcgtg gcgaaggcga tgccgccacg gataaactgg
     1861 ttaaaggtgc cggttttcac tacgccgccc gtcagaccaa agtccagact gtaacccgat
     1921 gccaaccaga agacggaatt gttacgcacc aggtgttgat agcgtttact gatgcgcatc
     1981 gcaatcatca cgcgatctga caatgtcccc agcgtcattc cggtaaccgt acctacttcc
     2041 agaccacgga acagcacagg cgtaccaata cctaacgaac cggcttccgg tgcttcgaca
     2101 ataatgctta agccatccag gtaacgcgaa tcagtaatag tagcttcttg caactcaaag
     2161 tcacggcgag gattaccccg acctggttcg acattgatat acggctggag gatagtatct
     2221 agatgctcaa cgcccgcggc tgaaatttgc ggtgtgacca ccgagaagcg cgtaccgccg
     2281 cgggcgaagg tctggacata ctccggatag agcaccgcct ttgcctgtac ttcattgcgc
     2341 gcggtaatca gatccagcgt ctggatttgc ccgatatcaa taccgagata gcgaattggc
     2401 atcccgaccg ccagttttcc ggcatcgaaa gcgtgaagcg taatctgccc accaaccgca
     2461 cgggccgctg tttcggaagc atacagaatt cgtttgtcgc ctttacgctg actggcgctg
     2521 gcaccgctga ggttatcgaa gctaatggct ccctttaatg ctctggagag cggggatgcc
     2581 tgtacggtca ggccactacc attcagctga actttcgccc cgccttctgc ccagaacacg
     2641 ctattgctgg tcagaaggtt gcgatactcc ggcttaatat gcagatcgat atcaaacgcg
     2701 ttagctcgcg gacgcacggt aatcacttca ccaacttcaa atttacggta cagcactacc
     2761 gagcctgcct gcacatccgg cagcgtctct gcactcaaac tcacggttgt ggtgggtaaa
     2821 tcgctaaggc tgttctccag cgctttttcc agattggcat acagtggata gctggctttc
     2881 atctcacctt tatcgcctgg cagaatacgt atcccgccgt taatccattc tgaggcgctg
     2941 gcaccaagaa actcaacgcc atccagcccc accttcacgt cgacacggct gttgacgaca
     3001 aatttgctat cgccttttac cagttcgcga tgctgaggct cgatggcgac ggtaaaggta
     3061 acgcctttgc tggtgagttt acgatcaata acctggccta cctgcacgcc gtgaagaatg
     3121 agcggctgac cagcatcaat accgtaactt tccggcgcgg tcagggtcag cgtcagaaca
     3181 tcaggttcat gcagcagtgc tttttcgcct ggcacaacaa cgaactcttt gcgtggctcg
     3241 ccatcgccgg gtaccaactc gaaggttttg ccagtcagca gggcgctgag attggcatcg
     3301 ctaagggata atttcggatt aagtaattcg atgcgggtat tttcacgaag cagggtaacg
     3361 acgctgggat taactgtcat ttccccagtg actttaccac caggatttaa atccagttta
     3421 gttagctgtc cgacttccag tccctgatac attaatggcg tcgagtcggc ggttaatccg
     3481 gccccactcg gcagttccag tttgattatt acgccacgct ggctatgggc gaggtcttca
     3541 tacagaccaa aggtatcttc cgcctcggca ggtttcgact cttcaggtga atcgaaggca
     3601 atcgcaccgt taaccagtgc cgccagactt tccagtttca ccttcgcgcc actgatactg
     3661 acgttggcat caacgccgga aacgttccag aaacggctac cttttttcac caggtcggta
     3721 aaacgccgct cgatcaggac atcaatcacc acgccttgct tgttgggatt gatggcatag
     3781 tcgtagactt ttcccacagg gatcttgcgg aaatagacca atgaaccgct gttcagcgaa
     3841 ccgagatcgg gggcttgcag gtggatcatc agatcgccat tgtccagtcg atatttcggt
     3901 tgggtatcga gtgcgacaaa gtgatcctgc tctttacctt tacctggcat catgccgata
     3961 tagttaccac cgacgagggc gtccagcccg gagacacctg ccaacgatgc ttttggcgtc
     4021 accagccaga actgagtctc ttcgcgcagc gcatctttca tatcggactt gatgctgacc
     4081 ttgacttcaa tcttacgaag atcgtcgctg aggctgatat cctgcactgt tccgacttca
     4141 acgccctgat aacgaacagg cgtacggccc gggacaatac catccgccga cataaagtcg
     4201 atggtgacgg tattaccccg gtcctgataa ctgtcccaaa tcagccaacc ggcaatcatt
     4261 agcgcgatga aaggcagcag ccagaaaggt gagatacggc gtttattttt aatctgcgct
     4321 tcagtcgtcg aagcgggcgt ttcctgactc atgtgcatcc caaagtaagc ggctgtccag
     4381 ccattccaca gcaagaatag tcaaaattac ggctgcgcca aaataaaacg cagccggtcc
     4441 catagtaaaa gcgaggatct gatcgcgatt aatcagcgac atggttaaag atatgacaaa
     4501 caggtcgagc atcgaccagc gtccaatcca ggtcaccatc cgcagtaaca gaatgcgtgt
     4561 gcgtaaacct tgctggcatt taaaatgaat gctcaacagt aaagtgaaca tgacgatcac
     4621 tttagtaaac ggtaccagaa tactggcgat aaacacgatt ccggcgaccg caatgttgct
     4681 acttgccagc gacataatcc cggaaagaat cgtatcttcc tgccggccac cattcagata
     4741 aatgatagaa ataggcaata ggttggctgg taacaacaaa acgattgacg ctaacagcgc
     4801 cgcccagcat ttttgcaaac tatgacgacg acgcaggcgt agcgggatat ggcaacgcgg
     4861 gcagcgacca cgctgatcag gatagccggt aaaatggcac ccaagacaga cacgaagttt
     4921 ctcgtcccta cgcgtagcgg ggcgctgcgg ataatatcgc tcccacagct cctcaacatt
     4981 aagatgtgac aacgtcaccg tcgttaaaat caccaacgcc acaaaagaga acaagccgac
     5041 acccgcctgg atatgggcat aatcctgtac ctttatagaa gcaacgccaa tgccgaccag
     5101 gtagatatcc agcattaccc actctttaag acgctcaagc atcagcagta ccggacgtaa
     5161 attcattccc agtcggttac caaaccataa atatgctatg gaggacacca gaatgagggg
     5221 ggcaccaata acgcagaaaa agaccatcgc ccccgttatc gtatcgccct gtttggtcat
     5281 ttgccagatg ccttgcatga cgttggcgtc gatacgaata cctaacagcc agatatgcaa
     5341 cagcggttcg ccccaggcaa acggcatcag caacagcatg gtgaaagcca ttgccgccag
     5401 gcgcgttagc gaccagtcgc gcccgtcgcg aatttttgcc tgacagcgcg gacaataggc
     5461 actctgatga gaatttatct cgggcaggct aaacagcatg tcacattgcg ggcaacgttg
     5521 gtaatcacca cgcggcagtt cctcgccgat tgccctcact gttatctttt ttgtcggcgt
     5581 aatttgtggt gtgttaagag ccatgacgtg ccaaatgtaa taagaattaa cattaatctt
     5641 aacccatgat gggaatgatc ttgagcatta acgcatttaa tgcttatttt aataggctgg
     5701 cagttccagg taagacaaac aagtgattat ataatgaaca aaacagaatt ttacgcggat
     5761 ttaaatcgcg actttaacgc gctgatggcg ggagaaacca gttttctggc aacgcttgcg
     5821 aacaccagtg cgttgttata cgagcgtctc actgacgtaa actgggcagg tttttatttg
     5881 cttgaggacg atacactggt actcggacca tttcagggca aaattgcctg tgtccggata
     5941 cccgtcgggc gcggggtgtg cggcactgcg gttgcccgca atcaagtgca gcgtatcgag
     6001 gatgttcacg cgtttgacgg gcatattgcc tgtgatgcgg cgagtaattc tgaaattgtt
     6061 ctgccgctgg tggtgaaaaa tcagattatt ggtgttcttg acatcgatag taccgtcttc
     6121 ggtcgcttta cagacgagga cgagcaaggc ttacgtcagc ttgtggcaca gcttgaaaaa
     6181 gtgcttgcaa cgacggatta caaaaaattc tttgcgagcg tcgcaggata atcaacggat
     6241 aacgtagcat ttactggtag cgtcattata atgacgcctg ttcatgcctg cgcttgttgg
     6301 ctacgtccgt tgtaatcagg aaatttcatg gaaaatcaac ctaagttgaa tagcagtaaa
     6361 gaagtaatcg cgtttctggc cgaacgtttt ccccactgtt tcagtgcgga aggtgaagcg
     6421 cgtccgctga aaatcggtat ttttcaggat ttggtcgatc gtgttgccgg ggaaatgaac
     6481 ctgagcaaaa cgcaattacg atccgcttta cgtctctaca cttcgagctg gcgttatctt
     6541 tacggtgtta aacccggcgc aacgcgtgtc gatcttgacg gcaacccatg cggtgagctg
     6601 gacgagcaac atgtagagca tgctcgcaag cagcttgaag aagcgaaagc gcgtgttcag
     6661 gcacagcgtg ctgaacagca agcgaaaaaa cgcgaagctg ccgcagctgc tggtgagaaa
     6721 gaagacgcac cgcgccgcga acgcaagcca cgtccgacca cgccacgccg caaagaaggc
     6781 gctgaacgta aacctcgtgc gcaaaagccg gtagagaaag cgccaaaaac agtaaaagca
     6841 cctcgcgaag aacagcacac cccggtttct gacatttcag ctctgactgt cggacaagcc
     6901 ctgaaggtga aagcgggtca aaacgcgatg gatgccaccg tattagaaat caccaaagat
     6961 ggcgtccgcg tccagctgaa ttcgggtatg tctttgattg tgcgcgcaga acacctggtg
     7021 ttctgaaacg gaggccgggc caggcatgaa catgtttttt aggcttaccg cgttagctgg
     7081 cctgcttgca atagcaggcc agaccttcgc tgtagaagat atcacgcgtg ctgatcaaat
     7141 tccggtatta aaggaagaga cgcagcatgc gacggtaagt gagcgcgtaa cgtcgcgctt
     7201 cacccgttct cattatcgcc agttcgacct cgatcaggca ttttcggcca aaatctttga
     7261 ccgctacctg aatctgctcg attacagcca caacgtgctg ctggcaagcg atgttgaaca
     7321 gttcgcgaaa aagaaaaccg agttaggcga tgaactgcgt tcaggcaaac tcgacgtttt
     7381 ctacgatctc tacaatctgg cgcaaaagcg ccgttttgag cgttaccagt acgctttgtc
     7441 ggtactggaa aagccgatgg atttcaccgg caacgacact tataaccttg accgcagcaa
     7501 agcgccctgg ccgaaaaacg aggctgagtt gaacgcgctg tgggacagta aagtcaaatt
     7561 cgacgagtta agcctgaagc tggcaggaaa aacggataaa gaaattcgtg aaaccctgac
     7621 acgccgctac aaatttgcca ttcgtcgtct ggcgcaaacc aacagcgaag atgttttctc
     7681 gctggcaatg acggcgtttg cgcgtgaaat cgacccgcat accaactatc tttccccgcg
     7741 taataccgaa cagttcaaca ctgaaatgag tttgtcgctg gaaggtattg gcgcagtgct
     7801 gcaaatggat gatgactaca ccgttatcaa ttcgatggtg gcaggtggtc cggcagcgaa
     7861 gagtaaagct atcagcgttg gtgacaaaat tgtcggtgtt ggtcaaacag gcaagccgat
     7921 ggttgacgtg attggctggc gtcttgatga tgtggttgcc ttaattaaag ggccgaaggg
     7981 cagtaaagtt cgtctggaaa ttttacctgc tggtaaaggg accaagaccc gcactgtaac
     8041 attgacccgt gaacgtattc gtctcgaaga ccgcgcggtt aaaatgtcgg tgaagaccgt
     8101 cggtaaagag aaagtcggcg tgctggatat tccgggcttc tatgtgggtt tgacagacga
     8161 tgtcaaagtt caactgcaga aactggaaaa acagaatgtc agcagtgtga tcatcgacct
     8221 gcgtagcaat ggcggtgggg cgttgaccga agcggtatcg ctctcaggtc tgtttattcc
     8281 tgcgggtccc attgttcagg tccgcgataa caacggcaag gttcgtgaag acagcgatac
     8341 cgacgggcag gtgttctata aaggcccgct ggtggtactg gttgaccgtt tcagtgcttc
     8401 ggcttcagaa atctttgccg cggcaatgca ggattacggt cgtgcgctgg ttgtgggtga
     8461 accgacgttt ggtaaaggca ccgttcagca ataccgttca ttgaaccgta tttacgatca
     8521 gatgttacgt cctgaatggc cagcgctggg ttctgtgcag tacacgatcc agaaattcta
     8581 tcgcgttaac ggcggcagta cgcaacgtaa aggcgtaacg ccagacatca tcatgccgac
     8641 gggtaatgaa gaaacggaaa cgggtgagaa attcgaagat aacgcgctgc cgtgggatag
     8701 cattgatgcc gcgacttatg tgaaatcagg cgatttaacg gcctttgggc cggagctgct
     8761 gaaggaacat aatgcgcgta tcgcgaaaga tcctgagttc cagaacatca tgaaggatat
     8821 cgcgcgcttc aacgctatga gggacaagcg caatatcgtt tctctgaact acgctgtgcg
     8881 tgagaaagag aataatgaag atgatgcgac gcgtctggcg cgtttgaacg aacgctttaa
     8941 acgcgaaggt aaaccggagt tgaagaagct ggatgatcta ccgaaagatt accaggagcc
     9001 ggatccttat ctggatgaga cggtgaatat cgcactcgat ctggcgaagc ttgaaaaagc
     9061 cagacccgcg gaacaacccg ctcccgtcaa gtaatatcaa tcaggcacaa gaaattgtgc
     9121 ctgatttttt aacagcggca agatgccgta aatcagatgc tacaaaatgt aaagttgtgt
     9181 ctttctggtg acttacgcac tatccagact tgaaaatagt cgcgtaaccc atacgatgtg
     9241 ggtatcgcat attgcgtttt gttaaactga ggtaaaaaga aaattatgat gcgaatcgcg
     9301 ctcttcctgc taacgaacct ggccgtaatg gtcgttttcg ggctggtact gagcctgaca
     9361 gggatacagt cgagcagcgt tcaggggctg atgatcatgg ccttgctgtt cggttttggt
     9421 ggttccttcg tttcgcttct gatgtccaaa tggatggcat tacgatctgt tggcggggaa
     9481 gtgatcgagc aaccgcgtaa cgaaagggaa cgttggctgg tcaatactgt agcaacccag
     9541 gctcgtcagg cagggatcgc tatgccgcaa gtggctatct accatgcgcc ggacatcaac
     9601 gcttttgcaa ccggtgcgcg ccgtgatgcc tctctggttg ctgtcagcac cggtttgctg
     9661 cagaacatga gcccggatga agccgaggcg gtaattgctc acgaaatcag ccacatcgcc
     9721 aatggtgata tggtcaccat gacgctgatt cagggcgtgg tgaacacctt cgttatcttt
     9781 atttcccgta ttctggcgca gcttgccgcg ggttttatgg gcggaaatcg tgatgaaggt
     9841 gaagagagca acggcaaccc gctgatctac tttgcggttg caacggttct ggaactggtg
     9901 tttggtattc tggcgagcat tatcaccatg tggttctcgc gtcatcgtga attccatgct
     9961 gatgccggtt cggcaaaact ggttggtcgc gagaaaatga ttgccgcgct gcagcgcctg
    10021 aaaaccagct atgaaccgca agaagcaacc agcatgatgg ctttctgcat taacggtaag
    10081 tcgaaatcgc tcagtgagtt gttcatgact cacccgccgc tggataaacg aattgaagct
    10141 ctgcgtacgg gtgaatacct gaagtaatat gaagtaaata ctaaaaagcg cgtcctgatc
    10201 gacgcgcttt ttttatgccc tggatcgtgg ctgagtgata cgtaaaccac tgacacaggc
    10261 agcaatcact gccagaatag ccgcagccat cagcgaaacg tgcgtaccat tatcaccgaa
    10321 ctgatttagc atcagcgcca ccagtgccgc gccgctactc tgacccagta gacgagccgt
    10381 tcctaacatg ccactggctc caccgctacg ttcgcgagga gcggaggtaa taatggtgtg
    10441 gttattgggt gactggaaca agccaaatcc agcgccacat aagatcatcg gccagataat
    10501 attgatatcc gcaggtgacg cgggcagcag aaccagggga aaaagccccg cagccatgat
    10561 gaacaaccct agagcaccca gcaatcctgc atgtacgcgt tcaatcaaat agcctgccaa
    10621 cggagccatc accatcgttg ctaacggcca cggtgtcaga agtaaacctg tttcgacttc
    10681 actacgcccg agcacggttt gcaggtaaaa gggcagggaa accattgcca gcatttgtgc
    10741 gcagaaagag caaacagatg tgcaaataga aagtgaaaac agcgggatac gcagtaaatc
    10801 caccggtagc agcggtacgg gaagagaaag ctggcggcga ataaagaaaa taccaacaac
    10861 aaccattacc accagttccg cagcaattaa cgtcagcgat tgcccctgag cgaaaccact
    10921 caacgcagtg ataagcaggc cgaaggttaa cgcgttcatc acggcgctgg gcaggtcgaa
    10981 acggggttta ctggcgcgag aaccattggg tggcagaaaa cgcatcgcca gaagcagggc
    11041 gataatacct aacggtacgt tgattaaaaa taaccatttc caggatgcga tggagaggat
    11101 tgctgcagca attgtcggcc cggcagcaga agagacggca acaatatggc gattgttggc
    11161 agggcgacgt tggcgattgc gccgtcaagg acagccatcg aaatgccaat cacaatggtt
    11221 aatatcgcac cgtatcgctg gggcaatggc aggccgtcgg cctgaacttt tggcatagga
    11281 attttatatc tttggtgaat aatcaacagg ggtatgattc tagcactgat attcacggtg
    11341 gatgtcgcag aattgtaact aaccggcaaa agatggattg cgaccaccct ggtgcgaaat
    11401 tataataaaa aacagttctg atttttataa aacactcgca atgaggtgat aaatggctaa
    11461 cgcagatctg gataaacagc ctgattctgt atcttccgtg ctaaaagttt ttggcatttt
    11521 gcaggcgctg ggtgaagagc gcgaaatagg gataaccgag ctgtcgcagc gcgtcatgat
    11581 gtcaaaaagc accgtttatc gctttttaca gaccatgaaa accttaggtt atgtggcgca
    11641 ggaaggggag tcggagaaat attccctgac cctgaaattg tttgaactgg gcgctcgcgc
    11701 gttacaaaac gtcgatttaa ttcgtagcgc agatatccag atgcgtgaga tctcccgcct
    11761 gaccaaagaa actatccacc tcggcgcact ggacgaagac agtattgttt acattcacaa
    11821 aattgactct atgtacaatt tgcgcatgta ttcacggatt gggcgtcgta atccgctgta
    11881 cagcaccgcg attggtaagg tactgctggc atggcgcgat ctcgatgaag tgaagcaaat
    11941 tcttgagggc gtggagtata aacgcagtac cgagcggacc atcaccagta cagaagcgtt
    12001 attacccgtt ctggaccagg tgcgcgagca ggggtatggc gaagataacg aagagcagga
    12061 agaagggctg cgatgcattg cggtaccggt atttgatcgc tttggcgtgg tcattgccgg
    12121 tttgagcatc tccttcccga cgttgcgttt ctctgaagag cgtttacagg aatatgtcgc
    12181 aatgttgcat accgcagcgc gcaaaatttc tgcccaaatg ggttatcacg actatccgtt
    12241 ctgatgagag taagaacctg tcggaatatc aaacagacag gttctttatt tagcatgaga
    12301 aaaataaagt tgaaggtgcg ttatattaaa cgcgcttgct ataagaatat tttactcatg
    12361 agtgagaatc tggttgttta ttacccttaa ccattatcga ccacgatatt gcttttacgt
    12421 aacagtgggc aatctgttat ccccaagaaa ccacttttag tgtgcaagta ttgtaccgtg
    12481 ctggtgcctc tggcagtcag ataggtacat tgcaaaccta atcctgcggc attctctttg
    12541 cttccaatca aaacgccata tccgctgagt aataatccta tccataccag tgctatcagc
    12601 ataactgtgc gaatgatgaa tcgcattaca acctcttctc tttttatgtt cgcttaatcg
    12661 tagcggcaat atgcgctgaa gcaagctact cattccgaaa aagcacgaat atcgacatag
    12721 ttaggcgctg tttaactaac gcatgctagt ttaatgacat aaggtaggtg aagcggagat
    12781 tggagtgaaa aagtttcgat gggtcgttct ggttgtcgtg gtgttggctt gcttgctgct
    12841 ttgggcgcag gtattcaaca tgatgtgcga tcaggatgta caatttttca gcggaatttg
    12901 tgccattaac cagtttatcc cgtggtgata tcattttttc agcactagat tttcttcctc
    12961 tccagcagtg gtagaatagc gcctctactc agaggtggtg aaatgaacga agttgtaaat
    13021 tcaggcgtga tgaacattgc gtctttggtt gtatcggtgg tggttcttct tatcgggctc
    13081 atcttgtggt tttttatcaa tcgtgccagt tcacggacta acgaacagat tgaactgctt
    13141 gaggcgttgc tggatcagca aaaacgtcaa aatgcactgt tacgtcgttt gtgcgaagca
    13201 aacgaaccag agaaagcaga taaaaagacc attgagagtc aaaaatcggt tgatgacgaa
    13261 gatattattc gcctggtcgc cgaacgataa tccttcctca atcttgagta accccgtcgt
    13321 tgaagggtta ctcaattcat cctgattata cgcactgtca tggtgcaact gcttcaaatt
    13381 aaaactctgg tctgacttca aataattatc atattattta tttgcgtaac gggatatcag
    13441 aaaaagatat tttcctcatt aatttattag gatgtttaca tcggatttgt gattaagcgt
    13501 ggtattattt attagcgaaa cgtttctctc ttgatttttt tgctcattca tcaatttttc
    13561 ttattttaaa ttttcaatcc ctttggggat tgacttctct ttagggtaat tgatagccgt
    13621 taaccgactg ttttatgaga aaaagtgata taacttttta ttcattgcat agcaaaaaat
    13681 gtgatattgc acgcactatg taataacttc tcccactggc ctggaacaac tgaacttatt
    13741 gaactatgtt agaaaatacg ccagtttaag tatctgcctg aactggcaag gttaagcaca
    13801 atgatatatc ggcgcgtatt ccgttgcata agtgtgcaaa aaaagtggaa gacgtatcga
    13861 gatttgtgcg tctgatcgag acatgtttaa aaatggcttg ccataattaa cgttgtatgt
    13921 gataacagat ttcgggttaa acgaggtaca gttctgttta tgtgtggcat tttcagtaaa
    13981 gaagtcctga gtaaacacgt tgacgttgaa taccgcttct ctgccgagcc ttatattggt
    14041 gcctcatgca gtaatgtgtc agttttatct atgttatgcc tgcgggcgaa gaaaacaatc
    14101 taaggaattt ttcaaatggc aaagattaaa ggtcaggtta agtggttcaa cgagtctaaa
    14161 ggttttggct tcattactcc ggctgatggc agcaaagacg tgttcgtaca cttctccgct
    14221 atccagggta atggcttcaa aactctggct gaaggtcaga acgttgagtt cgaaattcag
    14281 gacggccaga aaggtccggc agctgttaac gtaacagcta tctgatcgaa tccactgatc
    14341 tgaagtgtga atacgcttca atctcgctat aaagcctcgt cgaatgcgag gctttttact
    14401 atgctttatc ttcgctcctg gcgttcggat atttgcccgc cgcgtgattc gcgttacact
    14461 tgcggccttt agtatcctgc cggagttgtc atgtcttttt cctgtccact ttgccatcag
    14521 cctctttcgc gtgaaaaaaa cagctatatc tgtccccagc gacatcagtt tgatatggcg
    14581 aaagaagggt atgtcaatct gctgtccgtt cagcataaac ggtctcgtga tccgggcgac
    14641 agcgcggaaa tgatgcaagc acgccgcgca ttcttagatg ccggacatta tcagccgctg
    14701 cgtgatgcaa ttgtcgccca actgagggaa cggcttgatg ataaggccac ggcggtgctg
    14761 gatattggct gtggtgaagg gtattacaca cacgcatttg ccgatgcgtt gcccgaaatc
    14821 accacgtttg gtctggatgt ttcgaaggta gcgataaaag cggcggcgaa acgctatccg
    14881 caggtcactt tttgtgtcgc ttccagccac cgtttgccgt tttccgatac cagtatggac
    14941 gccataatac gtatttacgc gccgtgtaaa gcagaagaat tagcacgagt agtgaagccc
    15001 ggcggctggg tcattactgc cacgccggga ccgcgacatt tgatggagct gaaggggctg
    15061 atttacaatg aagtacatct tcatgcacct catgcagaac aactggaagg ttttacatta
    15121 cagcagagtg cggagttgtg ttatccgatg cgtcttcgcg gtgatgaagc cgtcgcatta
    15181 ttgcagatga cgccgtttac ctggcgtgcg aagccagaag tctggcaaac actggcagca
    15241 aaagaagtgt tcgactgcca gacggacttt aatattcacc tctggcagcg ttcttattaa
    15301 ccgtggaagt gcgtccagag gatctggacg ccgatgccga tcagtaccag cccgccgaga
    15361 atttccgctt ttttcccaat aattgagccg ataaagcgac caaccatcat ccctaatgtt
    15421 gacataatca aggttgcaca accaatggcc aatgcggtcg cgataatgtt gacctgcagg
    15481 aaagcaagac caacacccac agccatggca tccaggctgg tggcaatcgc ggtggttacc
    15541 agtagccaga aaccgtgtcg acggcgcggc tcttcatctt catcatctgc gccacgaaaa
    15601 ccctcaataa tcattcgccc gccgaggaat atcagcagca caaacgcaat ccagtggttc
    15661 cattcaagga caaaccggct ggctaacatg cccattcccc agccgatcag cggcgtcagg
    15721 gtttcgacgg caccaaaaat aaggccggtt cgcaatgctt cagaaaattt cggtttatgg
    15781 agggtggcac ctttaccgat tgatgcagca aatgcatcca tcgacatacc aaacgcaaga
    15841 agaacagtag cagtgatatt catgacaatg tcctgaccgg ggtatccata aaacacatca
    15901 tcgcccccag caaacatgca tacatgaaca acaagccgtg atggttttcg cgttcatgca
    15961 ggcatagcga ttgatgtgca tatggtctcg cctgactgaa agtattcagt ccgtacgtgc
    16021 cacgttttgc aacgagtatg ttgacacgta catttccgga atctggaaat cggctactcc
    16081 ccaaatgagg gcgcaacctt aacatattt
//
LOCUS       DAZPEP010000090        16091 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZPEP010000090 DAZPEP010000000
VERSION     DAZPEP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16091
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.090"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(174..326)
                     /locus_tag="ACHUT9_002746"
     CDS             complement(174..326)
                     /locus_tag="ACHUT9_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1349180.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(591..1325)
                     /gene="cysH"
                     /locus_tag="ACHUT9_002747"
     CDS             complement(591..1325)
                     /gene="cysH"
                     /locus_tag="ACHUT9_002747"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFR1349181.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1400..3112)
                     /gene="cysI"
                     /locus_tag="ACHUT9_002748"
     CDS             complement(1400..3112)
                     /gene="cysI"
                     /locus_tag="ACHUT9_002748"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFR1349182.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3112..4911)
                     /gene="cysJ"
                     /locus_tag="ACHUT9_002749"
     CDS             complement(3112..4911)
                     /gene="cysJ"
                     /locus_tag="ACHUT9_002749"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFR1349183.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5230..5592
                     /gene="queD"
                     /locus_tag="ACHUT9_002750"
     CDS             5230..5592
                     /gene="queD"
                     /locus_tag="ACHUT9_002750"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFR1349184.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5670..6941
                     /locus_tag="ACHUT9_002751"
     CDS             5670..6941
                     /locus_tag="ACHUT9_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1349185.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6932..7192
                     /locus_tag="ACHUT9_002752"
     CDS             6932..7192
                     /locus_tag="ACHUT9_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1349186.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7209..7784
                     /locus_tag="ACHUT9_002753"
     CDS             7209..7784
                     /locus_tag="ACHUT9_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFR1349187.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7932..8792)
                     /locus_tag="ACHUT9_002754"
     CDS             complement(7932..8792)
                     /locus_tag="ACHUT9_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1349188.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8789..9568)
                     /locus_tag="ACHUT9_002755"
     CDS             complement(8789..9568)
                     /locus_tag="ACHUT9_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFR1349189.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9546..10496)
                     /locus_tag="ACHUT9_002756"
                     /pseudo
     CDS             complement(9546..10496)
                     /locus_tag="ACHUT9_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417251.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(10483..11916)
                     /locus_tag="ACHUT9_002757"
     CDS             complement(10483..11916)
                     /locus_tag="ACHUT9_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFR1349190.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGINLAG"
     gene            complement(11986..12802)
                     /locus_tag="ACHUT9_002758"
                     /pseudo
     CDS             complement(11986..12802)
                     /locus_tag="ACHUT9_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13120..14397
                     /locus_tag="ACHUT9_002759"
     CDS             13120..14397
                     /locus_tag="ACHUT9_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFR1349191.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14424..15910
                     /locus_tag="ACHUT9_002760"
                     /pseudo
     CDS             14424..15910
                     /locus_tag="ACHUT9_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3807 a         4231 c         4040 g         4013 t
ORIGIN      
        1 atatatcatt caataaaata tttacattat gaataatcaa ataattttca tccgtaatat
       61 atatgaataa taaacatagg cataatattc acataaataa tataaattaa aaaagggtgc
      121 aggagaccaa accatcacaa ttcagtacgc ggggttttac ccccgcgtgg tggctacttc
      181 ttcgattcgt aagcgagaac tgccttaaac tccataccac gttctctgat actcaactca
      241 cacaacgagt cgcctaccat cagcgtgaat cccagtactg taaaacacag cactatgatt
      301 gccacaaggg catattttgt cagcatgtct tgcctccctg caaagaagag actaacattc
      361 gaattgtttg ggttcgaacg ctggcctcag gttgatagaa atatcgcctg gggcttttgt
      421 ccatctggaa cctcgcgaat gcttaacgcc agacagcctc aagcacccga cgccattcta
      481 tacctgataa ttcttccagc ggcgttcacg tttaaaatca ggaatactgc tcgcaaaata
      541 aaaacgcccc catcatttct gacagaggcg tttaatttat ccggcaatat ttacccttcg
      601 tgcaatccac attccctttt taagccaaag aaacgtgttt cttcttccga catgccaggt
      661 tcccatttac gggttgtatg ggtatcaccg accgataaat atccttcatc ccataatggg
      721 tgatatttca ggccatgttt ttgcaggtac tgataaatag ttcggttatc ccagtcgata
      781 atcggcagca ctttaaatac gccacgctga attgccagca ccggtaaatt ggcgcgactg
      841 ccggattgtt cacggcgcag gccagcaaac caggtttgcg cgttaagttc tttcagagcc
      901 cggttcatcg gttcgacttt gttgatgtca ttgtactttt caatgccttc aacgccctgc
      961 tcccacagtt ttccgtagcg tgcttcctgc caggctgcgc tttcggtagc acggtacact
     1021 ttcaggttga gcttgagttt gtccgttaac tcgtcaataa agcggtaggt ttccgggaac
     1081 aagtaacccg tatcggtgag gatcaccgga atatccgggc gaatttgatt caccagatgc
     1141 aggctcaccg ccgcctgaat gccaaagctg gaagaaagta catattcacc gggcagatta
     1201 tccagcgccc aggctacgcg gccttcagcg tccagttttt ccagttcggc gttagtttcc
     1261 gccagcgcca gaatacgatc taccttcggc agttcgttca gggcgtttag atcgagtttg
     1321 gacatagatt cctcactgtt tgccttgcct gatgcgacgc tcacgcgttc ttatcaggcc
     1381 tacaagaccg ggctgatggt taatcccaca aatcgcgcgc cggatcgagc accgggcgaa
     1441 tgatgcccgc acgcaccgta aagtcgccga agccttcacc cgcttcgcgc tctttcgccc
     1501 agcgccctat cagttcatca agcgacgcca ggatttccgg ctcggtgatg ttttctttat
     1561 acatccgtgg gatacgtgtc ccaatgcggt tgccgccaag atgcaggttg tagcgacccg
     1621 gcgctttacc caccaggccc acttccgcca gcatcgcgcg accacaaccg ttcgggcagc
     1681 ctgttacacg catcacgata tgctcatcgc tgacaccatg tttcgccatt aaattatcga
     1741 tgttgtcgat aaaagacggc aggaaacgct ctgcttccgc catcgccagc gggcaagtcg
     1801 ggaatgacac gcaggccatc gagttttcac gctgcggcgt gacggcattc attaacccgc
     1861 tctctttggc gatcttctcg atcttcgctt tctcgctttc cggtacaccg gtgatgatca
     1921 gattctggtt cgccgtaatg cggaaatcgc ctttgtggat cttcgcgatc tccagcaggc
     1981 cggttttcag cggacgcccc ggataatcaa ggatgcgacc attttcgata aacagcgtca
     2041 ggtgccagtt atcatcaatg cccttaaccc agccaatacg atcgcctcgt ccggtgaact
     2101 catatggacg gatcggttca aatttgatcc ccgcgcgacg ctccacttcc gctttaaacg
     2161 tctcaacccc cacgcgctcc agcgtgtatt tggttttggc atttttacga tcggttcggt
     2221 taccccagtc acgctgagtt gtcacgacgg cttcggccac cgccagcgta tgctccagcg
     2281 gcagatagcc aaactcgctc gccgtgcggg cgtaggtttt cttgttgccg tgttcgatag
     2341 aaagcccacc gcccaccagc aggttgaagc ctaccagctt gccgttttcg gcgattgcca
     2401 cgaagttcat gtcgttggca tgcagatcga tatcgttctg cggcgggatc actaccgtgg
     2461 ttttgaactt acgcggcagg taggtctgac caaggatcgg ttcttcatca gtagtggcga
     2521 ctttttcctg gtcgagccag atctccgcat acgcgcgggt acgaggcaac agatgctcag
     2581 aaatcttctt cgcccactcg tacgcttccg cgtgcagctg cgactcgtaa gggttcgagg
     2641 tgcagagtac gttacggttc atgtcgttag ctgtcgccag cgcatcaaga ccgaccgagt
     2701 gcagcatctg gtgcaccggt ttgacgttct ttttcagaat gccgtggaac tgaaacgtct
     2761 ggcggttggt caggcgaatg ctgccataga tggtgttttc accggcaaat ttgtcgatcg
     2821 cctgccactg tttagtggta atcaccccac ccggcagacg acagcgaagc agcatcgcgt
     2881 ggcgcggctc cagcttctgt tcagcacgtt cggcacggat gtcgcggtca tcctgctgat
     2941 acatgccgtg gaagcggatc agcaggaagt tgtcgccctt aaagccgccg gtcagaccgt
     3001 cgtttaaatc ttccgcaatg gtgccgcgca ggtagttgct ttcaagcttc atgcgctcgg
     3061 cgtctgtcag ttttccttcg accactaaag gccctggatg tttttcgctc attagtagac
     3121 atctcgctga taacggcgct ctacgcgcag ctcacttaaa aattcatccg ccgcttcggt
     3181 gtccatgcca ccaaattcgg caatcacttc cagaagtgcc tgctcaacgt ctttcgccat
     3241 gcgattagcg tcgccgcaga cataaatgtg ggcaccgtca ttgatccagc gccacagctc
     3301 cgcgccctgt tcgcgcagtt tgtcttgtac ataaactttt tctttttggt cgcgcgacca
     3361 ggcaagatcg atacgcgtca gcacgccctc tttgacgtag cgctgccact ccacctggta
     3421 gaggaaatct tccgtaaagt gcgggttgcc aaagaacagc cagtttttac ccggcgcttc
     3481 gtcggcggcg cgttgctgca taaaggcacg gaacggcgcg atgccggtac cggggccaat
     3541 cataatcact ggcgtttgcg ggttagcggg caggcggaaa ttgtcgttat gttcgataaa
     3601 tacgcggact tcgccctctt cttccacgcg atccgcgagg aagctggagg caccaccggc
     3661 acgggcgcgg ccttccacgt cgtaacgcac cacaccaacg gtgacgtgta cttcgttctc
     3721 gacttccgcc tgcgaggagg cgatggaata caggcgcggg gtcagcgggc gcagcaggtt
     3781 aatcagcgct tcggcatcca gttgcgccgg agagaaacgc accatgtcga caatcggcgt
     3841 ggtcgcggcg taatgctgta acttcgcttt atcgcccacc agcggcagca gcgtttcgct
     3901 gcgggtaagc gtggcatagt tctcaacaat gttggcggtg ttgacggtca gttcgaagtg
     3961 ccactgtagc gcttcgttca gaggcaacgt tttgccctcg acggtgacag gttcatcgcc
     4021 tttcagccac agcagttcga caagttcttt caccagtgcc gggtcgttct gataccagac
     4081 acccagcgcg tcacccggct ggtaacgcag gcctgaatcg cctaagtcaa tttcgatatg
     4141 acgaacgtct ttttcagagt tacgcccggt aattttctgg ttaaccgaaa ggctcgccac
     4201 cagcggcgcg tctttgctgt acgggctggt gtggatttca tttaccgcgc cagtagcgac
     4261 ggattgcgaa ggtgccgcga caggcgcacg cgatttaagc gcatcaacca cgcgggcgcg
     4321 ccactcgctg gcagcagcct ggtattcaac atcggcatcg acacggtcga gcaggcgttc
     4381 accacccagt tccgccagct tgctgtcgaa atctttcccg gactggcaga aaaattcata
     4441 agagctatcg ccgaggctaa acacggcaaa cgcggtgttt tccagctttg gcgctttttt
     4501 ggagaacagg aacttatgca gcgcgacggc ttcttccggc ggttcccctt ccccttgcgt
     4561 tgacgtcact acgatgagca gtttttcgct ggcgatttgt ttgaatttat agtcgcccgc
     4621 gttcaccagc ttaacgttca gttttgctgc taataaatca tcgcgtaatg cttcagcaac
     4681 ccggcgcgca ttgccggttt gcgaggcgga gataatagtt atacccggca tttctgcggc
     4741 tggcgctggc gtcgctgcaa gcgcagcagg ctgctgattg agtacgcccc agaaatagcc
     4801 agaaacccag gcaagctggg taggcgttaa atcggtcgtg gccgcctgaa ggcgtgccag
     4861 ttgctccggg ttcaacggaa gcaacgcgga aggtgggacc tgtgtcgtca tgcgtcgtta
     4921 tgttccagta agcaaagctg tttctgcgcc ctgtcagcgc ccataaaaca gaagagaaga
     4981 taaggttaac ggggtgaacg gtgtggatta aagacgggat agcgataact aataaccaaa
     5041 tcgactaacc tgttttagca atagtcttta acaacaaaat agattaacca acccaatgaa
     5101 aaacaaatga atttagccaa tcattaagat aaatcagcga ttttgcgcaa caaagtcgtt
     5161 ttagataatg cgaaaaaaca gcctttccgg tactctacgg cggttttatc gtcctgtaga
     5221 gaaattatga tgtccaccac gttatttaaa gatttcacct tcgaagccgc tcaccgctta
     5281 ccacacgtcc cggaagggca taaatgtggt cgcctgcacg ggcattcctt tatggtgcga
     5341 ctggaaatta ccggggaagt cgatccgcat acgggctgga ttatcgattt cgctgaactg
     5401 aaagcggcgt ttaagccaac ttatgagcgc ctcgatcacc attatctcaa tgatattcca
     5461 ggtctggaaa acccaaccag cgaagtttta gcaaaatgga tttgggatca ggttaaaccc
     5521 gttgtgccgc tgttaagtgc ggtgatggta aaagaaacct gcaccgcagg gtgtatctat
     5581 cgcggcgaat gataagagtg tgtcggcggt caatttccct taagtaacgc tatgttaggg
     5641 tgttgtgttc tggatatctg gggcatgaca tggaagacga ctgcgacatt attattattg
     5701 gtgccggtat tgcaggcacc gcttgcgcgt tacgctgcgc gcgagcgggt ttatccgttt
     5761 tgttactgga acgcgctgaa atccccggca gcaaaaatct ttccggcggg cggttatata
     5821 cccatgcact cgcggaactc ctcccgcaat ttcatctgac cgcgcctctt gaacgacgca
     5881 tcactcacga aagcctttcc ctgttaacgc ccgatggcgt aacgacgttt tccagcttac
     5941 agcccggcgg tgaatcctgg agtgtattac gtgcacggtt cgatccgtgg ctggttgccg
     6001 aagccgaaaa agaaggtgtc gaatgcatcc ccggagcgac ggtggatgca ctgtatgaag
     6061 aaaacggcag agtctgtggc gttatttgtg gtgacgatat tctccgcgcc cgttatgtgg
     6121 tgctggcaga aggtgccaac agcgtcctgg ctgaacgtca cgggttagtg actcgtcctg
     6181 ctggcgaagc gatggcgttg gggatcaaag aagtgctgtc gctggaaaca tccgctattg
     6241 aagaacgttt tcatctggag aataacgaag gcgcagcgtt gctgttcagc ggcggaatct
     6301 gtgatgactt acccggcggc gcatttcttt atactaatca acaaacgctc tcgttaggga
     6361 ttgtttgccc gctctcttcc cttacgcaaa gtcgtgttcc ggcaagcgag ctgctgactc
     6421 gctttaaagc gcatccggca gtgcgcccgc ttatcaaaaa cacggaatca ctggagtatg
     6481 gtgcgcatct ggagccagaa ggtggcttgc acagtatgcc ggtgcaatac gccggtaacg
     6541 gctggctgct ggtgggcgat gcgttgcgca gttgcgtcaa taccggaatt tccgtgcgcg
     6601 gcatggatat ggcgctgact ggcgcacagg cggcggcaca aacgctaata agcgcctgcc
     6661 agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca caacgtagag cgcagcctgc
     6721 tatgggatgt tctacaacgt tatcagcatg ttccggcgct tttgcaacgc cctggctggt
     6781 atcgggcgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata
     6841 aacctgttcc accgctgcgc cagttattct ggcgtcattt acgtcgtcat ggcctgtggc
     6901 atctggcggg cgatgttatc aggagtctgc gatgtctgta gcccataatc tctggcgcgt
     6961 tgctgatgcg ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaacggtt
     7021 gattaacgcc tgtccggcag gtcttttttc gctcacaccg gaaggtgact tacgcgttga
     7081 ttatcacggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca
     7141 acagtggcgc tatccgcctt ccggattcgg catcacctac cgctttggat aaataataag
     7201 gataatttat gcccctctta cacctactcc gccagaatcc ggtgattgct gccgttaaag
     7261 ataatgccag cctgcaactg gctatcgatt ctgaatgcca gtttatttcc gtgttgtacg
     7321 gcaatatctg caccatcagt aatatcgtta aaaagattaa gaacgccggg aaatatgctt
     7381 ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtcgttatt cagtttttga
     7441 aactggtgac cgaggcggac ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa
     7501 gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcaatt tcgtttcaca
     7561 acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgta
     7621 tgcccaaagt gctgggctgg gtgacagaga aaatccgcca accgctgatt gccggtgggc
     7681 tggtgtgcga tgaagaagat gcgcgtaatg cgattaacgc gggtgtcgtg gcgctttcca
     7741 ccacgaatac cggggtctgg acgttagcga aaaaattact ttgacgggat aatcgtaacc
     7801 aattgaattt ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg
     7861 acgcttgacg cgtcttatca ggcctacaag tccccaggcc agaaccgtag gcaggatcat
     7921 ccggcaacac gttattggca atctgcgtga atgtttgtca ccagtgcctc cagcaccact
     7981 ttccagtcat caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga
     8041 ttaatcgcca ccacaaactt gctattgcgt acgcccgcca ttaacgctgc tgcgccagaa
     8101 gcgccaacaa caatacacac ctcaggtgcc agcagatgtc cggaaatacc aatcactttt
     8161 tcagcatcaa cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc
     8221 ttttctgcca gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccagc
     8281 acccgtctgg cctcagctaa tgggtcgcga gtaacatttt tcagatcctc tgtgctaacc
     8341 agccagtccg gaagcgcacc cgggacaatg ttcagttgtt gcattccaga gggcaacgtg
     8401 gcgtttttcg ctgctcccgc ctgacgcgcc agggaaaggc atagcgggcg tttttctgtt
     8461 tgcagcgtcg ccgttagcgc atttccccag tgggatttgc gcacgcttac ggttggaata
     8521 tctaacgaag tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgggtcgac
     8581 agttcatcgc caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac
     8641 tgttccacca acgcatccag cacctgttcc gccactacag gctgaggttc aatctgccag
     8701 tgcgccaggg tacaaccgct gaaatcctgc gccgccagcc agcttgctat tgcggcattc
     8761 tcctgattta ttgtcacaat tgcgatattc atggctgcat cctttggcgt aaatagtcct
     8821 gccatagctt ttgagctttt tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc
     8881 gttgctctgc tcttgccagt tgcagacact gcatcgctgg catttcggca gcaacagttt
     8941 tacggataat ttctgctttc cccgcagcca ttcgctggcg cattcccggt actggcaagg
     9001 ccacttcacc gcactgacgc acggcaatca ccgcaggcaa gcgaacccgg caacaacgca
     9061 acccatgttc agtacgctgt tcgagggtga taaacagcgc gtcgagagtg aaacgctcca
     9121 cctgggtgaa gcagggccag cccagcattt ccgccagcaa aaatggcgtt tgcccattct
     9181 gcccttcgct gctttggcag ccggtaatta tcagatccag cggattctga tgctgccatt
     9241 cggcaatatg gcgagcgaca aattccggcg caaagcgcag atctgccgcc gtctccagca
     9301 atacagcttc ctcaaaccca agagccatga gatagcgcag ccagtgcaat gcccgttcat
     9361 cccccatgct caacgcggtt aaagacatcg gcgtaccgtt ttttctctgc gccaacagca
     9421 gcgcggcagc ggcctgttca tcagcaccga gtaaacttcg cagtagcgaa atatccggtc
     9481 cgcttttacc ctgagccgcc gcctgccact ctttttccgc cagcattccg gcatccggtt
     9541 cggctttaaa cgctaacaga atgttcattc gcacctccta catttcccgc cgccaccagc
     9601 gggagggctt tagtttctgg tgcccatagc caggtcacaa caaaatcaac caacaacact
     9661 gtcgccagga gcaataaggt gacttgcatt ccccactgcg ccagcaccca cggcagcagg
     9721 ccagtactta ccgccgcgcc aagtcgactc atggcggtgg caaaaccgac gcccagcgag
     9781 cgaatgtcag taggaaaact ttccgcaggc aaaatgccca ccagattact gactgccgaa
     9841 atggtggtgc tgaagagaac aaaaagcagc agcgttaatg aactgccgga aggcaaacag
     9901 gccatcacta ccagcgttgc cgccagcagc aaaaaacttc ccagcaaaaa tttgcgatgt
     9961 gccagcaggt gcgtcagaac taatcccagc agcgcgccca caattaacaa cgcattaagc
    10021 atcaggctgg cagtcagcgc atcttccaga ccaatagtct gggcgatagt tggcagccag
    10081 gtataaatca caaaccatgg gattacgagg cagacaaaga agacgctgtt aaacgccgtg
    10141 cgccgccagt aacgcgaaga gaacaaggtt ttgatgtgtt tatgggtcgc cgttaccact
    10201 tcatcgccca gtaaaacatg gggaccaaaa tagcgatgca cgatagcgtg agcttctgca
    10261 aaacgcccct ggcgcagtag ccagcgtggt gattctggcg ttccccagcg taataacgta
    10321 atcaacaacg cgggcagagc tgccgatgcc agtagccagc gccaggcctc cgggttttcg
    10381 gaaataaagt gatgtccggc aatacttgcc agcacatagc ctacggtcca caccacgctg
    10441 aatgcgccca gcaaaatacc gcgatggcgg cgcggggaaa attcagccag caaggttaat
    10501 gccattagga tcgaactgct ttttcagccc ttccagcaac gcccacgcgc tgccgtgttc
    10561 cagtttgctc cagtgaacgc gatgtttacc gataccgtgg tggtgcacca tcgaaccgcc
    10621 gaggcgaatg gtttcttcac agatgatctt gttgagcgga ttgtggtact tgtcgatttc
    10681 ctcttccggc ttacagtcaa cgacgttgta atcgtagacg aagtacatgt tggtgccgtt
    10741 ctgatagcta tgagaggaat gaccgcccag catggtgatg tcgtcggcgt gcgggaactc
    10801 agtacgaata cggttaataa cgctttcgta gatttcgtgg atgcagctcc agcagccgga
    10861 cacttcagtg gtaaagccca tgttgccggt tttgaggatc tgcacacgtt cggcagccac
    10921 tttatccggt ccccagttca ggttgttaaa ccaggtttcg atcagcttgc tgtccacgcg
    10981 ctggcattgc gggtagcggg caacgatttc cgcaatccct tcgcccgtcg ccttcgcaat
    11041 gcgagggtta ccttcagcca taaagatcag cacgcatttt ccgtctgcaa aatgggtgaa
    11101 gtgttgggtg ccatcttcag cgtcatacaa acgagcgatc gacggacgat acccttccac
    11161 catgacttca cgcaggatgt tgaagtcggt tttcatgtct tccaggatat agccgtagaa
    11221 gaggttgttt tccggggtga atttaaagat tttcactgtt acttcagtga tatagcaaag
    11281 tgcaccttcg ttaccgatga tgatgtggcg aatgtccggg ccagccgcgc ggcgtggcac
    11341 gtttttaatg cgtgtgacgg tgccatcagc caatactgct tccagaccaa cgaccatatc
    11401 ttcgattgcg ccgtagagtg tggagaactg cccgatactg cgggttgcta ccaagccgcc
    11461 catctgcgcc agcggctttg actgcggaga atgccccgtg gtgtaacctt tttcacgcaa
    11521 cgcattttcc agcacctcca gcggaacgcc acattgcgcc gtcgcctgca tattctcaat
    11581 atcaatatta atgatttgat tcatggcgga gccgtcaagc accaccgagt tttccacaac
    11641 ggtttccagc ccaccttcag tggcggaagc gccggtacgc ggcacaccgt taattttgtg
    11701 cgcattcata aaattcagca cacgggatac ttgctctgtg gaaccgagtt ttacgaccgc
    11761 tgccggaatc ggcaaagtat aaatgccatg aatatccgga aatttacgaa aacggtcaat
    11821 actgttcttc tttaatactg tttcatcggt aattacgcga tctgcaccaa caatttcctt
    11881 tagttggtcg acaatcgctg cgcgagataa agacatagta attccttcct gataataaaa
    11941 atggaataat cattaataag agaaaacaga aaccaccgtt atggattagc gcactaaata
    12001 accaccattg accactaata aatgcccatt gacataattt gaagccggac ttgcgaggaa
    12061 tacagctgcg cccattaaat cctgagtatc gccccaacgg tttgccggaa tatgatcaag
    12121 aacgcgctga ttggtttctg gattactgcg tgtcgccagc gtaatatctg ttgcataata
    12181 gccaggagca ataccattta cctgaatatt atattgacct agttcatcac aataagcttt
    12241 ggtgaatccg gcaagagcat gtttagtggc agaatatgca ggtgaccatt gtccacctaa
    12301 gtaagagaac aatgaacaga tattaataat tttgccgctt ttctgcggga tcataatttt
    12361 tgcagcttca tagcttaact cgaatgcggc ggtcaggttc acatcaatca tcggatccca
    12421 gtcggcacga ccgaagtcca gcaccttatt cagcttacaa ataccggcat tgtttaccag
    12481 aatatcaact gtaccgaaac gctcacagca agcagcgata atcttctgcg gcgcgccttc
    12541 tgcggtgata tccacctgca tgaagtccac ctcaacaccc tgtttttcaa acatttcctt
    12601 tgtttcgccg ttatctttca atcatttcct ttgtttcgcc gttatctttg acgaaactag
    12661 gaataaagat atttgcgcca gctttggcca acgccatggc aaatgcctgg cctaaaccgc
    12721 tattcccacc ggtaacaatt gcggttttac ctttcagtga gaaaaaatcc attgagaacg
    12781 cattgagaga ttcgattgac atagttagct ccaatttccg taaggcaaaa aaaagaaagg
    12841 taacttcccc cgcgagaggg aagttacctt tctcattatc tctggtaact ggagataatt
    12901 tttagcataa cggcataact gctaatgtga tcgtaatcac agtgtgatat tcgtattgtc
    12961 aaatagtttt aaaaagcaga agcgtgatta ctctcacaca acacctctgc tttccatgtt
    13021 atttcttatt gcaattacta gagacctgag aaaagtaatc cacctgaaag ggcggcttac
    13081 ttttctcttt tttttgtcta ttacaaatgg gaatacgata tgcaacacaa ctcatatcgc
    13141 cgttggataa ccctcgcgat aattagtttt agcggcggcg ttagttttga cctggcttat
    13201 ttacgttata tttatcaaat tcccatggcg aaatttatgg gattcagcaa taccgagata
    13261 ggtttaataa tgagtacctt tggtattgcg gctattattc tttatgcccc cagcggcgtt
    13321 attgccgata aattttcaca ccgcaaaatg attacttccg cgatgatcat taccggatta
    13381 ctgggtctgt taatggcaac gtatccaccg ctgtgggtaa tgctctgtat tcaggtcgcc
    13441 tttgcgataa cgacgatttt aatgctgtgg tcggtgtcga ttaaagccgc ctcgttgctg
    13501 ggcgatcata gtgagcaagg gaaaattatg ggctggatgg aagggctgcg cggcgtcggt
    13561 gtaatgtcgc tggcggtgtt taccatgtgg gtcttttctc gctttgcacc ggatgacagc
    13621 gccagcctga aaacagtcat tatcatctac agtgtggttt acatcttgtt ggggattctg
    13681 tgctggtttt ttgttagcga taacaacaac ctgcgcagtg ccaataacga agaaaaacag
    13741 tcattccagc ttagcgacat ccttgccgtt ttgcgtatca gcaccacctg gtattgcagc
    13801 atggtgattt ttggtgtctt cacaatctac gccattctga gttactccac caactatctg
    13861 accgaaatgt acggcatgtc gctggtggca gcgagctaca tggggattgt gatcaacaaa
    13921 atcttccgcg cgctgtgcgg cccacttgga ggcattatca ccacctacag taaagtgaaa
    13981 tcccctaccc gcgtgattca aatcctttcc atcatcggtt tgctggcgtt gacagccctg
    14041 ctcgtcacga actctaaccc ccaatcggtc gcgatgggga ttggcctgat tttactgctg
    14101 ggattcacct gttacgcctc acgcgggctg tactgggcct gccccggcga agcgagaaca
    14161 ccgtcttaca ttatgggcac cacggtaggt atttgttcgg tgattggatt cctgccggat
    14221 gtcttcgttt acccgattat cggccactgg caagacaccc tgcccgctgc agaagcctac
    14281 cgcaatatgt ggctgatggg catggcggcg ctcggcatgg tgattgtctt tacctttttg
    14341 ctgttccaaa aaattcgtac tgctgatagc gcccccgcaa tggctagcag caagtaagcc
    14401 aggaaaatta caggagaaaa atcatgtcga aaaaatacat catagggatt gatggcggaa
    14461 gtcagagcac aaaagtggtg atgtacgatc tggaaggtaa tgtggtttgc gaaggtaaag
    14521 gcttattaca gccgatgcac acgcctgatg ccgatactgc agaacatcct gacgacgatt
    14581 tatgggcatc attatgtttt gcaggtcacg atttgatgag ccagtttgcc gggaataaag
    14641 aagatattgt tggcatcggc ctgggctcca tccgttgctg ccgtgcgtta ttgaaagccg
    14701 acggcacgcc agctgcgccg ttgattagct ggcaggatgc acgcgttaca cgcccttacg
    14761 aacatacgaa tcctgacgtg gcgtatgtaa cctctttttc gggttatctg acgcatcgct
    14821 taaccggcga atttaaagat aatatcgcca actattttgg tcagtggccg gtggattata
    14881 agacctgggc atggagcgaa gatgccgcag tcatggagaa gtttaatatc ccccgtcata
    14941 tgctgtttga tgtgcaaatg ccaggcactg ttcttgggca tatcacacct caagccgcgc
    15001 tggcgacgca tttcccggca ggactgccgg ttgtttgtac caccagtgat aaaccggtag
    15061 aagctctggg agccggatta ctggatgatg aaactgcggt aatttctctt ggcacttaca
    15121 tcgcattgat gatgaacggc aaagccttgc cgaaagatcc ggtggcgtac tggccgatta
    15181 tgtcttctat tccgcaaaca ttgctgtatg aaggttacgg tattcgcaaa ggcatgtgga
    15241 cggtgagctg gctgcgcgac atgttaggcg agtcgttaat tcaggatgcc agggcgcagg
    15301 atctttcacc ggaagattta ctcaacaaga aagcctctag tgtgccacct ggctgtaatg
    15361 ggctgatgac ggtgctggac tggctgacca atccgtggga accgtacaaa cgcgggatta
    15421 tgatcggctt tgattccagc atggattacg catggatata tcgttcgata ttggaaagcg
    15481 tggcgctgac gctgaagaac aattacgaca atatgtgtaa tgaaatgaat cactttgcga
    15541 aacatgtaat cattactggc ggcggttcga acagcgatct gtttatgcag atttttgccg
    15601 acgtgttcaa ccttccggca cgccgtaacg ccattaacgg ttgtgcaagc ctgggggcag
    15661 cgattaatac aggggtaggt ctggggctat acccggatta cgcaacggct gtcgataaaa
    15721 tggttcgcgt gaaagatatc tttataccga ttgagagcaa tgccaaacgc tacgacgcga
    15781 tgaataaagg cattttcaaa gacctaacca aacatactga tgtgatcctg aaaaaatcgt
    15841 atgaagtgat gcatggggaa ttggggaatt ggggaatgcg gattcgatcc agagctggtc
    15901 gaatgcgtga ataaggtagg ccgggcggaa aactgcccgg cctgaacata cctgaatggt
    15961 tatccccgct ggcgcgggga acataagttc gacaacttaa aaaatgataa caagtccggt
    16021 ttatccccgc tgatgcaggg aacataataa aaactttaac tagttcggtt tatccctgtt
    16081 tttacaagga a
//
LOCUS       DAZPEP010000091        16048 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZPEP010000091 DAZPEP010000000
VERSION     DAZPEP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.091"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHUT9_002761"
     CDS             complement(36..1859)
                     /gene="typA"
                     /locus_tag="ACHUT9_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFR1349192.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            2232..3641
                     /gene="glnA"
                     /locus_tag="ACHUT9_002762"
     CDS             2232..3641
                     /gene="glnA"
                     /locus_tag="ACHUT9_002762"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFR1349193.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            3815..4864
                     /gene="glnL"
                     /locus_tag="ACHUT9_002763"
     CDS             3815..4864
                     /gene="glnL"
                     /locus_tag="ACHUT9_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFR1349194.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            4876..6285
                     /gene="glnG"
                     /locus_tag="ACHUT9_002764"
     CDS             4876..6285
                     /gene="glnG"
                     /locus_tag="ACHUT9_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFR1349195.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            6397..6507
                     /locus_tag="ACHUT9_002765"
     CDS             6397..6507
                     /locus_tag="ACHUT9_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFR1349196.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHUT9_002766"
     CDS             complement(6697..8070)
                     /gene="hemN"
                     /locus_tag="ACHUT9_002766"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFR1349197.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHUT9_002767"
     CDS             complement(8259..8768)
                     /gene="yihI"
                     /locus_tag="ACHUT9_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFR1349198.1"
                     /translation="MKPSSSNSRSKGHAKVRRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(8887..9053)
                     /locus_tag="ACHUT9_002768"
                     /pseudo
     CDS             complement(8887..9053)
                     /locus_tag="ACHUT9_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9350..9982
                     /gene="yihA"
                     /locus_tag="ACHUT9_002769"
     CDS             9350..9982
                     /gene="yihA"
                     /locus_tag="ACHUT9_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFR1349199.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHUT9_002770"
     CDS             complement(10364..13150)
                     /gene="polA"
                     /locus_tag="ACHUT9_002770"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFR1349200.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            13514..14446
                     /locus_tag="ACHUT9_002771"
     CDS             13514..14446
                     /locus_tag="ACHUT9_002771"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFR1349201.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(14487..15917)
                     /locus_tag="ACHUT9_002772"
     CDS             complement(14487..15917)
                     /locus_tag="ACHUT9_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1349202.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         3804 a         4097 c         3941 g         4206 t
ORIGIN      
        1 agcgatggca ggttttttat aactaaaaaa cgaaattaat cgtctttcgg tgcgcggttg
       61 gcgcgacgac gatcgttttc cgtcaggtga cgtttacgaa tacggataga ggtcggagtc
      121 acttctacca gttcgtcatc atcgatgaac tccagagctt gttccagagt catgcggata
      181 ggcggaacca gaacaacagc ttcgtcagta ccggaagcac gcatgttggt cagtttctta
      241 ccggtcaggc agtttacagt caggtcgtta gagcggctgt gaataccgat aatctgacct
      301 tcgtaaactt ctgcaccgtg accgaggaac agcttaccgc ggtcctgcag accgaacagt
      361 gcgaacgcga ccgctttacc ctgaccgtta gagatcagta cgccgttctg acgctgaccc
      421 acttcacccg gacgtacgtc gtcgtagtgg ctgaaggtgg agtacagcag accagtaccg
      481 gaagtcatgg tcatgaactc agaacggaag ccaatcagac cacggcttgg gatcacgtag
      541 tcgagacgta cgcggccttt accgtctgga ttcatgtttt tcaggtcgcc tttacgttcg
      601 cccagcgcct gcattacaga accctgatgc tgttcttcaa cgtccagagt cacgttttca
      661 tacggctctt gtttacgacc gtcgatttca cggaagatta cttttggacg ggataccgcc
      721 agttcgaaac cttcacgacg catgttttcg atcagaacag acaggtgcag ttcgccacga
      781 ccagaaacgc ggaacgcatc ggcgtcttcg gtttcttcta cgcgcagcgc aacgttgtgt
      841 accagttctt tgttcagacg atccaggatc tgacgagacg ttacgaactt accttcttta
      901 ccgcagaacg gcgaggtgtt aacgcagaag aacatagaaa cggtcggctc atcaacggag
      961 agtgccggca gcgcttcaac gttttgcgtg tcgcaaacgg tgtcagaaat gttcagttcg
     1021 ccaaggcccg tgatcgccac gatatcgcca gcttccgcca gatcggtttc gatacgttcc
     1081 agaccgaggt ggcccagcac tttaccgact ttcgcgttgc gggttttgcc ttcgctatcg
     1141 atgatagtga cctgctggtt cggcttcact ttaccgcgct tgatgcggcc aatgccgata
     1201 acgccaacat agctgttgta atcgagctga gaaatctgca tctggaacgg accgtcaagg
     1261 tcaacgtccg gcgcaggaac gtggtcaaca atcgcctggt acagcggggt catgtcttcc
     1321 gccatatctt cgtggtccag acccgcgata ccgttcagcg cagaagcgta aacgatcggg
     1381 aagtccagct gctcgtcggt cgcgtcgagg ttaacgaaca gatcgaatac ctgatccaca
     1441 acccaatcag gacgcgcgcc agggcggtca actttgttga taacaacaat cggcttcagg
     1501 ccgtaagcaa acgctttttt ggttacgaag cgcgtttgcg gcatcgggcc gtcaaatgcg
     1561 tcaaccacca gcagcactga gtctaccatg gacattacac gttcaacttc accaccgaag
     1621 tcggcgtgcc ccggggtatc aacgatgttg atacggtaat cattccattt gatagcggtg
     1681 tttttcgcga ggatggtaat cccacgctct ttctccaaat cgttggagtc catcacgcgc
     1741 tcttgggttt cggcacgaga gtcgaacgta ccggattgtt ggagcagctt gtctaccagg
     1801 gtggttttac catggtctac gtgcgcgatg atggcgatat tacgcaattt ttcgatcaca
     1861 actttgcctc aggcattaga aatagcgcgt tattgtacac ggattaatcg cactacaaaa
     1921 caggatcaca aacatcctcc gcaaacaagt attgcagagt ccctttgtga tcgctttcac
     1981 ggagcataaa aagggttatc caaaggtcat tgcaccaaca tggtgcttaa tgtttccatt
     2041 gaagcactat attggtgcaa cattcacatc gtggtgcagc ccttttgcac gatggtgcgc
     2101 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta
     2161 cggcgacacg gccaaaataa ttgcagattt cgttaccacg acgaccatga ccaatccagg
     2221 agagttaaag tatgtccgct gaacacgtac tgacgatgct gaacgagcac gaagtgaagt
     2281 ttgttgattt gcgcttcacc gatactaaag gtaaagaaca gcacgtcact atccctgctc
     2341 atcaggtgaa tgctgaattc ttcgaagaag gcaaaatgtt tgacggctcc tcgattggcg
     2401 gctggaaagg cattaacgag tccgacatgg tgctgatgcc agacgcatcc accgcagtga
     2461 ttgacccgtt cttcgccgac tccaccctga ttatccgttg cgacatcctt gaacctggca
     2521 ccctgcaagg ctatgaccgt gacccgcgct ccattgcgaa gcgcgccgaa gattacctgc
     2581 gttccactgg cattgccgac accgtactgt tcgggccaga acctgaattc ttcctgttcg
     2641 atgacatccg tttcggatca tctatctccg gttcccacgt tgctatcgac gatatcgaag
     2701 gcgcatggaa ctcctccacc caatacgaag gtggtaacaa aggtcaccgt ccggcagtga
     2761 aaggcggtta cttcccggtt ccaccggtag actcggctca ggatattcgt tctgaaatgt
     2821 gtctggtgat ggaacagatg ggtctggtgg ttgaagccca tcaccacgaa gtagcgactg
     2881 ctggtcagaa cgaagtggct acccgcttca ataccatgac caaaaaagct gacgaaattc
     2941 agatctacaa atatgttgtg cacaacgtag cgcaccgctt cggtaaaacc gcgaccttta
     3001 tgccaaaacc gatgttcggt gataacggct ccggtatgca ctgccacatg tctctgtcta
     3061 aaaacggcgt taacctgttc gcaggcgaca aatacgcagg tctgtctgag caggcgctgt
     3121 actacattgg cggcgtaatc aaacacgcta aagcgattaa cgccctggca aacccgacca
     3181 ccaactctta taagcgtctg gtcccgggct atgaagcacc ggtaatgctg gcttactctg
     3241 cgcgtaaccg ttctgcgtct atccgtattc cggtggtttc ttctccgaaa gcacgtcgta
     3301 tcgaagtacg tttcccggat ccggcagcta acccgtacct gtgctttgct gccctgctga
     3361 tggccggtct tgatggtatc aagaacaaga tccatccggg cgaagccatg gacaaaaacc
     3421 tgtatgacct gccgccagaa gaagcgaaag agatcccaca ggttgcaggc tctctggaag
     3481 aagcactgaa cgaactggat ctggaccgcg agttcctgaa agccggtggc gtgttcactg
     3541 acgaagcaat tgatgcgtac atcgctctac gtcgcgaaga agatgaccgc gtgcgtatga
     3601 ctccgcatcc ggtagagttt gagctgtact acagcgtcta atagttgaag ttgtactacc
     3661 cggcgcaaca acgccgggat ttagttgccg tggaaacttt cagcccatct ctgcatgggc
     3721 ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag ctataatgca
     3781 ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc agcccgatgc
     3841 tgggcagatc ctcaactcgc tgattaacag tattttgtta attgatgaca atctggcgat
     3901 ccattacgcc aaccccgccg cgcaacaact gctcgcccaa agctcccgca aattgtttgg
     3961 tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga tgcaagaaag
     4021 tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg acgggcgctc
     4081 gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc tggagatggc
     4141 tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc agcaggttgc
     4201 tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg gaggtttacg
     4261 tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg aatataccaa
     4321 agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt tggggccgca
     4381 gctgcccggt acgcgcgtta ccgaaagtat tcacaaagta gctgaacgcg tggtgacgct
     4441 ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgacc ccagcctgcc
     4501 ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg tgcgcaatgc
     4561 gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca ccgcgtttca
     4621 actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg aagataacgg
     4681 gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca gcggccgcga
     4741 aggtggcacc gggcttggct tatccatcgc ccgtaatttg attgatcagc attcaggcaa
     4801 aattgaattt accagttggc caggtcatac cgagttctcg gtttacctgc ctatcaggaa
     4861 ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga tagttccatc
     4921 cgttgggtgc ttgaacgtgc gctcgctgga gcgggtttaa cctgtacgac atttgagaac
     4981 ggcgcggaag tactggaggc gctggcgagc aaaacgccgg atgtgctgct ttcagatatc
     5041 cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg ccatccaatg
     5101 cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag cgcctatcaa
     5161 caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt ggcgctggtt
     5221 gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatattca gcttaacggc
     5281 ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg tattatcggt
     5341 cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac cggtaaagaa
     5401 ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt tatcgcgctg
     5461 aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca cgagaaaggc
     5521 gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga tggcggtaca
     5581 ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt gctgcgcgtg
     5641 ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt ggatgtgcgt
     5701 attatcgctg ccactcacca gaatctcgaa caacgagtgc aggaaggtaa gttccgtgag
     5761 gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg cgaacgtcgg
     5821 gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga actgggcgta
     5881 gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc gtggccaggc
     5941 aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc cgggcaggaa
     6001 gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga gagtacttcg
     6061 caaatgcaac cggacagctg ggcaacgctt cttgcgcagt gggcagacag agcgctgcgt
     6121 tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac gttactgacg
     6181 accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact cggctggggc
     6241 cgcaacaccc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac agcttgtgtg
     6301 taaagattga ttattgagcg caaattgctg gtattttacg ctttactgtt ccgataagtt
     6361 cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat aattaatctg
     6421 gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc cgtgctgtgt
     6481 gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc tctttgttca
     6541 tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc aacaggtttt
     6601 gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg atggcgcttt
     6661 cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg agaactgctg
     6721 catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc ggatcagcaa
     6781 gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc catcttttgc
     6841 taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca aatcccactg
     6901 tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa tcacatcgcg
     6961 gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc cttgttcatc
     7021 cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt cgccaatcat
     7081 gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg tgtagccctg
     7141 gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat ccggacgggc
     7201 aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga tggtttcctg
     7261 caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt tgcgctgagc
     7321 agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg ggttcagctc
     7381 cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca ggccgtaaat
     7441 caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca gtgcaaagat
     7501 gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga agtcctgcac
     7561 gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat ccagttcgat
     7621 ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga agttttcgcg
     7681 cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg ttccgccgcc
     7741 ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga cgatttcttg
     7801 ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa tcttattgca
     7861 accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag ataatggacg
     7921 ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt ctgaaaactc
     7981 cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct ggatcagggc
     8041 cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg tctctggcgg
     8101 ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag ccacttttgt
     8161 cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat aaccggaagg
     8221 gttatcagca gaacgggaag gcccacggtg gcaatccgtt agttaccccg cagcagacgc
     8281 atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata agagaggccg
     8341 agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga ttgctcttcg
     8401 gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg ctcatccgtt
     8461 tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt cggtttgtgc
     8521 tgtttggtga ctttttcagc cacgcccaat ggaatagggg ttttactgcc aatacgtgga
     8581 tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc tgcgcggctg
     8641 cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc ctgatccagc
     8701 tcctcgcgtg tttttcgacg cacttttgca tgacctttgc tgcgtgagtt tgaagatgat
     8761 ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg gcggaatcta
     8821 acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt tcctgactca
     8881 taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt tttcctttag
     8941 ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg tttgtcttcc
     9001 tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt cattgttcct
     9061 gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa acaatcaacc
     9121 gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg atttatatag
     9181 cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact atcggcaatg
     9241 tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg ccttttatta
     9301 tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt tgactaattt
     9361 gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc taccttccga
     9421 taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca gcgcgctgaa
     9481 cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc gcacccagct
     9541 tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg ggtacggtta
     9601 tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg aatacctcga
     9661 aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc cgctgaaaga
     9721 tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc tggtgctgct
     9781 gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata tggtgcgtga
     9841 agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt tgaagaaaca
     9901 aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc agcctgtaga
     9961 agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat gtggcgtatc
    10021 cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat agcgcatcag
    10081 gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact gactggggcg
    10141 gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga tagaacatct
    10201 tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga aagcactttt
    10261 tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa acgtgacagc
    10321 ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc gcctgatccc
    10381 agttttcgcc actccccact tccaccagca acggcacatc cagacgggta cagttttcca
    10441 tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact tcaaatacca
    10501 gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt aaccacgcat
    10561 caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt ggcgcgttaa
    10621 tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata tccggcagat
    10681 acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga gcacgggtgc
    10741 gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg tacttctgcg
    10801 cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg ccataaatca
    10861 gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt tccagtggca
    10921 aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg aatgcggtca
    10981 gcaagccttt gtcacgcgaa agatgcgcca taatgcgcag ttcaatctgc gagtagtccg
    11041 ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga cgaccttctt
    11101 cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt cccgttgcag
    11161 ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc atcagcggca
    11221 gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga atcacttttg
    11281 gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac ggcgcgccac
    11341 ccggcgtttt cttcagcggt ttaatgccct gtttttcaaa cagaatggtt tgtaactgct
    11401 tggtggaaga aaggttaaat tcctcacctg caatttcatg cgctttcttt tccagctcag
    11461 ccagacgaag ggtgagctct tcagaatgat tgtgcagcac tttcggatcg atcttcacac
    11521 cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc tcgaagacgt
    11581 tcaacggccc tttgtgtttt tgcagatctg gccacatttt cagatgcaac tgcaaggtga
    11641 catccgcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg ttaaaggtca
    11701 gttgattttt gcctttaccc gcaatctctt caaaagtgat agttttgtgc ttcaaccaac
    11761 gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg taggactcca
    11821 gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga ataccgcgat
    11881 cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc ggttttagca
    11941 actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca tgagcaaccg
    12001 gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg ttagcagaga
    12061 tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt tccagcttcg
    12121 caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca taagaaatca
    12181 ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc ttcgcggctg
    12241 gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc cagcgtttga
    12301 actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc acttccagtt
    12361 gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg tatgagagat
    12421 aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca cggaagctca
    12481 acccagcaat tttttctggc tcggcataca gcgtatccag cccgccaaga ccttgcagca
    12541 atgcctgcgc ggttttttca ccgacgcccg gtacgccagg aatgttatcg gaggagtcac
    12601 ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta ttcaccacct
    12661 cttccggtcc gaggatggta ttcgtcatgg tgttgataag cgtaatattt ggcgtcacca
    12721 gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct ttttcggctt
    12781 cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc agcagcggca
    12841 gtcccatcgc tttaaccatc gcgtgcaggg gttcgatttg tgctcgcaga tcgtccggca
    12901 ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt tttcccttgg
    12961 cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg cgcagcatgt
    13021 tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc gggggaaacg
    13081 cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga ttttggggga
    13141 tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc cacagacagg
    13201 atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct tctggatcgc
    13261 tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt ttaatttatt
    13321 atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa aataaagcca
    13381 tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgga ttatttttct
    13441 ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct tgcgcttctt
    13501 tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa tactcgctgc
    13561 gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt gttccgtccc
    13621 gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt ggcgaaaggt
    13681 ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg tattactgca
    13741 tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta agaagaactg
    13801 gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt gcgtgctgtt
    13861 tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct gggtgccatt
    13921 tcttggcctg gcgtgctggg cgctggatat gccgtttatg aagcgttatt cccgcgctta
    13981 tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc gttcttgtga
    14041 aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc gtttcacgca
    14101 agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa aggcggcagg
    14161 cattgcgatg gcgctaaatg tactgggtaa acaattcgat aaactgctta atgttacgct
    14221 gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta aactgacgcg
    14281 gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg actacatcaa
    14341 tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc aggaaaaaga
    14401 ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt tactcgcctc
    14461 tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg agacataaat
    14521 tcttcttcgc tcatctcctg tccgttaaac acaactttac ccggtgtata acgcagctgc
    14581 agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa tgtcatcatc
    14641 ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc ttcagataaa
    14701 ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc aaaattgagc
    14761 aatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat actgatatcc
    14821 agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt aatggtcggc
    14881 tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac ttcttgcaga
    14941 gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat agcaatgtta
    15001 tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat caatgaccac
    15061 tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta ggtcagattg
    15121 acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac gaaagcatcg
    15181 atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt cgactcgctt
    15241 tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt aaaacttttt
    15301 gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt gccttcaata
    15361 tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc accaaaagaa
    15421 aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc accaaacgcg
    15481 aatcgcaatg tcgcttccac gggcgattta tttttcgcgg cgataaatag cggttgtgtc
    15541 agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac attaccatgc
    15601 attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac aactttctgg
    15661 cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat ttgaaaacga
    15721 gaattaaaaa aacctttgtc aaaattttta taacttaacg tcatatcgta ggcatgttca
    15781 cctttctttt tagcatcgtt aaaatcttta ataaactttt cgacaccagg ctgaatttgc
    15841 gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat aacacctgta
    15901 gctgacttac gtatcatata aatattccct gataaaacag caaaacttta aatatccgct
    15961 tatgctgaaa taatgtccag cggcaggacg cattatcaga atgtatttat atttattaaa
    16021 taataaaaaa aaccgtgaat attcacgg
//
LOCUS       DAZPEP010000092        15963 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZPEP010000092 DAZPEP010000000
VERSION     DAZPEP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.092"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUT9_002773"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="ACHUT9_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFR1349203.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUT9_002774"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="ACHUT9_002774"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFR1349204.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUT9_002775"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="ACHUT9_002775"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFR1349205.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUT9_002776"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="ACHUT9_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUT9_002777"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="ACHUT9_002777"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFR1349206.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     IYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUT9_002778"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="ACHUT9_002778"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFR1349207.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUT9_002779"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="ACHUT9_002779"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFR1349208.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="ACHUT9_002780"
     CDS             8229..8939
                     /locus_tag="ACHUT9_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1349209.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUT9_002781"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="ACHUT9_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFR1349210.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="ACHUT9_002782"
     CDS             9636..10343
                     /locus_tag="ACHUT9_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFR1349211.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="ACHUT9_002783"
     CDS             10343..10891
                     /locus_tag="ACHUT9_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFR1349212.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="ACHUT9_002784"
     CDS             10901..12067
                     /locus_tag="ACHUT9_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFR1349213.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="ACHUT9_002785"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="ACHUT9_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter permease"
     gene            13576..14229
                     /locus_tag="ACHUT9_002786"
     CDS             13576..14229
                     /locus_tag="ACHUT9_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFR1349214.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUT9_002787"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="ACHUT9_002787"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFR1349215.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15963)
                     /locus_tag="ACHUT9_002788"
     CDS             complement(15612..>15963)
                     /locus_tag="ACHUT9_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1349216.1"
                     /translation="LFYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAA
                     KHQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTR
                     VWSGYLTLKSLQRQ"
BASE COUNT         3700 a         4343 c         4165 g         3755 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagatatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaagc ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatggc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctagatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag atcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 aga
//
LOCUS       DAZPEP010000093        15931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZPEP010000093 DAZPEP010000000
VERSION     DAZPEP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.093"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..173
                     /locus_tag="ACHUT9_002789"
     CDS             <1..173
                     /locus_tag="ACHUT9_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFR1349217.1"
                     /translation="RNRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRF
                     GMTPGEYRRKFR"
     gene            complement(345..674)
                     /locus_tag="ACHUT9_002790"
     CDS             complement(345..674)
                     /locus_tag="ACHUT9_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFR1349218.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(679..864)
                     /locus_tag="ACHUT9_002791"
     CDS             complement(679..864)
                     /locus_tag="ACHUT9_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFR1349219.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(861..3500)
                     /locus_tag="ACHUT9_002792"
     CDS             complement(861..3500)
                     /locus_tag="ACHUT9_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFR1349220.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHUT9_002793"
     CDS             3708..4697
                     /gene="ldhA"
                     /locus_tag="ACHUT9_002793"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFR1349221.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHUT9_002794"
     CDS             4808..5230
                     /gene="hslJ"
                     /locus_tag="ACHUT9_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFR1349222.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5227..5493)
                     /locus_tag="ACHUT9_002795"
     CDS             complement(5227..5493)
                     /locus_tag="ACHUT9_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFR1349223.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHUT9_002796"
     CDS             5767..9291
                     /gene="nifJ"
                     /locus_tag="ACHUT9_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFR1349224.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9657..10790
                     /gene="ompN"
                     /locus_tag="ACHUT9_002797"
     CDS             9657..10790
                     /gene="ompN"
                     /locus_tag="ACHUT9_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFR1349225.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10931..11365
                     /gene="uspF"
                     /locus_tag="ACHUT9_002798"
     CDS             10931..11365
                     /gene="uspF"
                     /locus_tag="ACHUT9_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFR1349226.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11951..12865
                     /gene="sitA"
                     /locus_tag="ACHUT9_002799"
     CDS             11951..12865
                     /gene="sitA"
                     /locus_tag="ACHUT9_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFR1349227.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12865..13692
                     /gene="sitB"
                     /locus_tag="ACHUT9_002800"
     CDS             12865..13692
                     /gene="sitB"
                     /locus_tag="ACHUT9_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFR1349228.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            13689..14546
                     /gene="sitC"
                     /locus_tag="ACHUT9_002801"
     CDS             13689..14546
                     /gene="sitC"
                     /locus_tag="ACHUT9_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFR1349229.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14543..15400
                     /gene="sitD"
                     /locus_tag="ACHUT9_002802"
     CDS             14543..15400
                     /gene="sitD"
                     /locus_tag="ACHUT9_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFR1349230.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(15601..>15931)
                     /locus_tag="ACHUT9_002803"
     CDS             complement(15601..>15931)
                     /locus_tag="ACHUT9_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1349231.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKLSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
BASE COUNT         3986 a         4023 c         3829 g         4093 t
ORIGIN      
        1 ttcgtaaccg tcgtctcgat ttttgtgcag atgcgattcg ccatgccgca gatgatgaaa
       61 aactggcagg catcggcttt cattggggat tttctgacca gagtcatttt tcaacggtat
      121 ttaagcaacg ctttgggatg acgccaggcg agtatcgacg taaattccgt taactaattc
      181 gcgttgtgtg tactgcaacg ccaattagtt aaaacagata tttgactatg catttttcag
      241 ggcaaaaggc aatttgacag gagtttttga atattcacct tgtttcagga aaatagatca
      301 atcgctttct cctgtagcgt tatcccgata ttgcggtttt gctattaaaa ttttcgcacc
      361 catttaccgt ttatcccttg tacatattcc cccggtttgg cccgagctac gagcttttgc
      421 ccggcgagtt tcgcgatatc atctaccgac acattatttt gctttgccag ttgttggtaa
      481 ctcgctttac gttcggcatt aatgtcggtt actaatttct ctgtttcagc atccgttttc
      541 agcgcaacca gataaccata aaatgtttca cctacccgcc cctgagttct ggcttcatcc
      601 agagtcaatg ccattacatt gctgcttacc agcccaacaa gaaacgcaca aagaagtaat
      661 gttttcttca tcatcgcctc agaaaagatc gctacgagtt tcaagcagct cttcgacatc
      721 tttgtctgcc ttgatgatga tctcatgctc aattttaacg ttcatattga tagtgatcgg
      781 ttccttgggc gcagcgactt caatacgcgg cgtacagcca accagcataa aagatgacgt
      841 caacgcagcc agtaaaattt tcattgtttt tcctcacact cttttccttg cggacagtca
      901 tttcccggca gacgtgcgtt ctgctccaac catgcctgga gattatcgcc aaagcgtaaa
      961 ctgcgccaca gcgtaaaaat attctcttca tgatggtaat taaggtttac cgtaccgctt
     1021 ttaccatcaa cgcgactggt acctgtaatg ttggcctgca tggttaataa accgagatta
     1081 tctaagttaa tttttgtcga tgaccggcta atttccatat agcgcaacca gttaattgct
     1141 gaacccgcag tcatattgtc tttcaccacc gcatctgcgg tgtctttatc cagccgcaag
     1201 gtcattggcc cgctatttgc cagccaacca tctttcacta tccatttttc gttgtttagc
     1261 cataacggca atgcaccact aaatgccccg gacatggcga attgtttcgg attgacggcg
     1321 ctaaccagtt cgctggatga gagattattc agccgcaaca gcgccgggtc atgttgcggc
     1381 atacgtaatt gtttcatcag tacgttaccg cctaacacat cgacgctaac atcagtcaac
     1441 agcaagggtt cttcttcagt ccacggataa cgcccttgca aatcagccgt aatatttttc
     1501 gctgtcacca gattaatcac ttcggcaatt cgcaacgtaa cggggccgcg agtccccaga
     1561 tgccaggctc catcggcaaa acggaaaggc aggacaaaat cgacaccgtt aacctgatta
     1621 tctggcatcc aggcactacc gcctttcaac acgccgtgtc ctcccgcgcg gaatccttgt
     1681 tcaggcgcag cagaaaatgc aacctgagca tacagttccc catcgcgtaa gttcatcttc
     1741 cagtcgggtg gcaccagcgg ctggaataca gtcagtgatt gttttggcca ccaggcgtta
     1801 ccgcgcagac gaataccgtc ccagcgacca tttacccgaa ccgggccaat ctcaccagca
     1861 tgtaaatcgc ctttaaagag gaaatacgtc ggatcgcgcc catcgacgct aaattttaag
     1921 gttgatggcg gtaacacact gccgccagtg aacagcgttt gcccggcgtc cagtgacagc
     1981 gcgccgctga aggagggatg ctgtgcgtca cgtacccaga cgacgggctt gtcgagaatt
     2041 aatcgcggct tttctaccgt catcgtaccg tattgtaact ggtcgaaacc ggtggagagg
     2101 tcggtcagcg taatcgtgct gtcatgccat tcaccttttc ctgcgacatc ccaggtggca
     2161 tttatcggtg taaaactgcc ttttccccag tagcgccact gccagcggcc cgcgtcaggg
     2221 agaaaatcat tcgccagccc atccatatgc agcacgaaat cgcccagttc atattcatgc
     2281 gcctgcaaaa tggcctgcaa acgtccgtca acaccacgtt gggtgacttt tacacccgct
     2341 aaaggccagc ggatttcatc gatgtccagc gaatcgatga ctcttccctt cgaacgtaat
     2401 aacgcgcctg gctcaaaggc cagcgttggg tcagtcagac ttccacttag ctgcgcgggt
     2461 aagcgggcat ataaaatgag gtccgcctgt ttcgcttcac cggtcagctg cagaggcagc
     2521 tggctgttat ccatgcttaa ttttcctggg ccaaaattta gcaccgcgtt gcctttaccc
     2581 gcttgcccct gggtcagcac actcagtcgt ccgctgatca gagcgttctc aagccctgcc
     2641 tgccagttgt cgactttgac acccagtcgg ccactcaaag gaaaacctgc atacggccaa
     2701 ctccagcgac catcgcttac ggtcaattgt tgacgagtaa tttgccacgg caaatcgagc
     2761 aacggatcgc cgttatcccg tgccagcaca atcaattgcc cgctattttc ctgccagtcc
     2821 agctctgcat ccaccagtga cggttcctgc ggcaagttta acgtcgcagt agcatgccca
     2881 cttacaggaa gtccatccgg cacgagcggc atagtaaatt cccccaccag tttaaccggc
     2941 ggctgatttt caaacgcgat gacatccagt tcgctgactg taagttgttg ccctttcagc
     3001 tggccttgaa atttaacttt ttcgccctga taacgcagtt gctgaatatc agaggttaat
     3061 gcgagagaga gttttccctg ccattcctgc cagggagaaa aaatcagttt atcgatattg
     3121 atccaggtgt taggcagcat ggactgccac tgcgcgaggg tttttggagc agctggcgat
     3181 tgctccgtct gcggcaattt cgccagacaa gcagaatcaa gttctaccat gccgacgttc
     3241 aataaccagc ggctgggatg tgaaaggctg gcgttggtga tatgcgcaag ctgacaatct
     3301 cccaccagat aacggagatc gggaatgatt aaacctttac gcgtaatgcg tgggctttca
     3361 tctaatgcaa tacgtgtccc gagcggtagc cagatgcccg ccagcgtggg aacccacagc
     3421 ccgagcgtca tcagcagcgt caacggcaca agaataatca gtaataacag cgcgagaacg
     3481 gctttatatt tacccagcat gggtagttaa tatcctgatt tagcgaaaaa ttaagcattc
     3541 aatacgggta ttgtggcatg tttaaccgtt cagttgaagg ttgcgcctac actaagcatt
     3601 gttgttgatg aatttttcaa tatcgccata gctttcaatt atatttgaaa ttttgtaaaa
     3661 tatttttagt agcttaaatg tgattcaaca tcactggaga aagtcttatg aaactcgccg
     3721 tttatagcac aaaacagtac gacaagaagt acctgcaaca ggtgaacgag tcctttggct
     3781 ttgagctgga attttttgac tttctgctga cggaaaaaac cgctaaaact gccaatggct
     3841 gcgaagcggt atgtattttc gtaaacgatg acggcagccg cccggtgctg gaagagctga
     3901 aaaagcacgg cgttaaatat atcgccctgc gctgtgccgg tttcaataac gtcgaccttg
     3961 acgcggcaaa agaactgggg ctgaaagtag tccgtgttcc agcctatgat ccagaggccg
     4021 ttgctgaaca cgccatcggt atgatgatga cgctgaaccg ccgtattcac cgcgcgtatc
     4081 agcgtacccg tgacgctaac ttctctctgg aaggtctgac cggctttact atgtatggca
     4141 aaacggcagg cgttatcggt accggtaaaa tcggtgtggc gatgctgcgc attctgaaag
     4201 gttttggtat gcgtctgctg gcgttcgatc cgtatccaag tgcagcggcg ctggaactcg
     4261 gtgtggagta tgtcgatctg ccaaccctgt tctctgaatc agacgttatc tctctgcact
     4321 gcccgctgac accggaaaac taccatctgt tgaacgaagc cgccttcgat cagatgaaaa
     4381 atggcgtgat gatcgtcaat accagtcgcg gtgcattgat tgattctcag gcagcaattg
     4441 aagcgctgaa aaatcagaaa attgggtcgt tgggtatgga cgtctatgag aacgaacgcg
     4501 atctattctt tgaagataaa tccaacgacg tgatccagga tgacgtattc cgtcgcctgt
     4561 ctgcctgcca caacgtgctg tttaccgggc accaggcatt cctgacagca gaagctctga
     4621 ccagtatttc tcagactacg ctgcaaaact taagcaatct ggaaaaaggc gaaacctgcc
     4681 cgaacgaact ggtttaatct tgccgctccc ctgcattcca ggggagctta ttcagataat
     4741 ccccaaagac ctttcatctt ctattcttaa aatagccctg agtcagaaac tgtaattgag
     4801 aaccacaatg aagaaagtag ccgcgtttgt tgcgctaagc ctgctgatgg cgggatgtgt
     4861 aagtaatgac aaaattgctg ttacgccaga acagctacag catcatcgct ttgtgctgga
     4921 aagcgtaaac ggtaagcccg tgaccagcga taaaaatccg ccagaaatca gctttggtga
     4981 aaaaatgatg atttccggca gcatgtgtaa ccgctttagc ggtgaaggca aactgtctaa
     5041 tggtgaactg acagccaaag ggctggcaat gacccgtatg atgtgcgcta acccgcagct
     5101 taatgaactc gataacacca ttagcgaaat gctgaaagaa ggtgcacaag tggatctgac
     5161 cgcgaaccag ttaacgctgg cgaccgcgaa acagacatta acttataagc tggcggattt
     5221 aatgaattaa tagctgccac agctcccggc ggcaagtgac tgttcgctac agcgtttgcc
     5281 gttgggtaat gcacacatcc caatcgccgt accatccagt tgacgggcaa cagaaagcga
     5341 accgccgatc attgcacaat ttgcttctcc actactggac atcgacgctt ttaaacctgg
     5401 cgctacgtgc gccgcagtcg cctgctgaac aggttcacta ctacacgccg acaacaataa
     5461 agcggcacac cctacccaaa acgctgctcg catctcttct tcctctgatc ttcaagccaa
     5521 acgacaccgc cataaataat aggcagcaca gagggcggcg tcgagagctg tcctgcgcgt
     5581 tgccccgcat ttttactttt ttatggctat ttttttgccc tctgtttgat caaaacattc
     5641 attacgctga tgtgggggac acaaaagcga aaatgcagaa gaaagccatt tgctaaaatt
     5701 gaaggattac tactgggcgc gcagcaattt cgtgcgcccc tcatttgcgc aatgtaaggg
     5761 tgtcatatga ttactattga tggtaatggc gcggttgctt cggtggcgtt tcgcaccagt
     5821 gaagttatcg ccatctaccc tattaccccc agttccacga tggcagaaca ggctgatgcc
     5881 tgggccggaa acggcttgaa gaacgtttgg ggagacacac catgcgtggt tgaaatgcag
     5941 tcggaagcgg gtgctatcgc taccgtgcat ggcgctttgc agacgggtgc cctttcaaca
     6001 tcgtttacgt catcgcaggg tttgctgctg atgatcccga cactgtacaa actggcaggc
     6061 gaactcacac cgtttgtcct gcatgtagcg gcacgtaccg ttgccacaca tgcactctct
     6121 atttttggcg atcattccga cgtcatggcg gtgcgccaga cgggttgcgc gatgttgtgt
     6181 gcggcaaacg tccaggaagc gcaagacttt gctctcattt cgcacatcgc gacgctgaaa
     6241 agccgcgtgc catttattca tttctttgat ggtttccgca cgtcccacga aatcaacaaa
     6301 attgtcccgc tggccgatga cacgattctt gatctcatgc cgcaggccga aattgatgct
     6361 catcgcgccc gggcgctcaa cccggaacat ccggtgatcc gtggtacgtc cgccaatcct
     6421 gacacttatt tccagtctcg cgaagccacc aacccctggt acaacgcagt ctatgaccat
     6481 gttgaacagg cgatgaatga tttctctgcc gcgacaggtc gtcagtatca gccgtttgaa
     6541 tattacgggc atccgcaagc tgaacgggtg attatcctga tgggctcagc cattggcacc
     6601 tgtgaagaag tggttgatga attgctaacc cgtggcgaaa aagtcggcgt gctgaaagtt
     6661 cgcctgtacc gccccttctc agccaaacac ttgctacaaa ctctaccggg atccgtacgc
     6721 agcgtggcgg tactggacag aaccaaagaa cccggtgccc aggcagaacc gctctatctg
     6781 gatgtgatga ccgcactggc agaagccttt aataatggca agcgcgaaac tctgccccgt
     6841 gtcattggtg ggcgctatgg tctttcatcc aaagaatttg gcccggactg tgtactggcg
     6901 gtatttgccg agctcaacgc ggctaaaccg aaagcgcgct ttacggttgg tatttacgat
     6961 gatgtaacca atctgtccct gccgttgccg gaaaacaccc agccaaactc ggcgaaactt
     7021 gaagccttgt tttatggcct tggtagtgat ggcagcgttt ccgcgaccaa aaacaatatc
     7081 aagattatcg gtaattccac gccgtggtac gcacagggct attttgttta cgactccaaa
     7141 aaagcgggcg gcctgacggt ttctcacctt cgcgtgagcg aacagccgat tcgttccgct
     7201 tatctcattt cccaggctga ttttgttggc tgccaccagt tgcagtttat cgataaatat
     7261 cagatggctg agcgtttaaa acctggcggc attttcctgc tcaacacgcc gtacagcgca
     7321 gatgaagtgt ggtcgcgctt gccgcaagaa gttcaggccg tgttaaacca gaaaaaagcg
     7381 cgcttctatg tgattaacgc ggcgaaaatc gcccgcaaat gtggcctggc ggcccgtatt
     7441 aataccgtca tgcagatggc ttttttccat ctgacgcaaa ttctgcctgg cgatagcgcc
     7501 ctcgcagaat tgcagggtgc gattgccaaa agttacagta gcaaaggcca ggatctggtg
     7561 gaacgcaact ggcaggctct ggcgctggcg cgtgaatccg tagaagaagt tccgttgcaa
     7621 ccggtaaatc cgcacagcgc caatcgaccg ccagtggttt ccgatgccgc ccctgatttc
     7681 gtgaaaaccg taaccgcagc gatgctcgca gggcttggtg atgccctccc cgtttcggcg
     7741 ctgccgccag acggcacctg gccgatgggc accactcgct gggaaaaacg caatatcgcc
     7801 gaagagatcc ccatctggaa agaggaactc tgtacccaat gtaaccactg cgtcgcagct
     7861 tgcccacact cagctattcg cgcaaaagtg gtaccacccg aagcgatgga aaacgcccct
     7921 gccagcctgc attcgctgga tgtgaaatcg cgtgatatgc gcgggcagaa atatgtcttg
     7981 caggtggctc cggaagattg caccggttgt aacctgtgcg tcgaagtttg cccggcgaaa
     8041 gaccgtcaga atccagagat taaagccatc aatatgatgt cgcgcctgga acatgtcgaa
     8101 gaagagaaaa tcaattacga tttcttcctc aacctgccag aaatcgaccg tagcaaactg
     8161 gaacgtattg atattcgtac atcgcagctg attacaccgc tgtttgaata ttccggtgct
     8221 tgctccggtt gtggcgagac gccgtatatt aaattgctga ctcagctcta tggcgaccgg
     8281 atgttgatcg ctaacgccac cggctgttct tcaatttatg gcggtaacct gccctctaca
     8341 ccgtatacca ccgatgccaa cggtcgtggg ccggcatggg cgaactctct atttgaagat
     8401 aatgccgaat ttggccttgg tttccgcctg acggtcgatc aacaccgtgt ccgcgtgctg
     8461 cgtctgctgg atcaatttgc cgataaaatc ccggcggaat tactgacggc gttgaaatca
     8521 gacgctacgc cagaggttcg tcgagaacag gtcgcagctt tacgccagca actcaacgat
     8581 gttgccgaag cacatgaact gctacgtgat gcagatgcac tggtggaaaa atcaatctgg
     8641 ctgattggtg gtgatggctg ggcttacgat atcggctttg gcggtctgga tcatgtattg
     8701 agtttgacgg aaaacgtcaa cattctggtg ctggatacgc aatgctattc caacaccggt
     8761 ggtcaggcgt cgaaagcgac accgctgggt gcagtaacta aatttggcga gcacggcaaa
     8821 cgtaaagcgc gtaaagatct tggcgtcagt atgatgatgt acggtcatgt ttatgtggcg
     8881 cagatttctc tcggcgcgca gctgaaccag acggtgaaag cgattcagga agcggaagcg
     8941 tatccgggac catcgctgat cattgcttat agcccgtgtg aagagcatgg ttacgatctg
     9001 gcactcagcc atgaccagat gcgccaactc acagctaccg gcttctggcc gctatatcgc
     9061 ttcgatcctc gtcgtgccga tgaaggcaaa ctgccgctgg cgctggattc acgcccgccg
     9121 tcagaagcac tggaagaaac gttacttcac gagcaacgtt tccgtcgtct gaattcgcag
     9181 cagccagaag tggcagaaca gttatggaaa gatgctgcag ctgatttgca aaaacgctat
     9241 gacttcctgg cacaaatggc cggaaaagcg gaaaaaagca acaccgatta aatgctctgg
     9301 ataaggatta cccaattcta aaaaaaagcc cggacgactg ttcgggcttg tctttttata
     9361 tgttggaaaa tcagtggcaa tgcaatggcc caacagaaaa taaaatctgt gacaataaag
     9421 gcatataacc cgcgcagaat aacgtataga aaattaaaag tataattttt attttgtatg
     9481 tattatcttc gatacattca ccagacttat tatcatttca gaagaattat ttgattaagg
     9541 ttttacttaa ggcgtaacaa atgatattta tcggctaact gaacttctcc tttaggatgt
     9601 tttcactccc cggaatgggg ataaaaggca aataaaataa ctaaaggatt tattcaatga
     9661 aaagcaaagt actggcactt ttaattcctg ccctgctcgc cgcaggtgct gcacatgcag
     9721 ccgaagttta taataaagac ggcaacaaat tagatctgta tggcaaagtt gatggcctgc
     9781 attatttttc tgataattca gcgaaagatg gcgatcagag ctatgcgcgt ctgggtttta
     9841 aaggcgaaac ccaaattaac gatcaactca ctggctacgg tcaatgggaa tacaatattc
     9901 aggcaaacaa cactgaatct tcaaaaaacc agtcatggac ccgtctggca tttgccgggc
     9961 tgaaatttgc agattacggt tctttcgatt acggacgtaa ttatggcgta atgtacgaca
    10021 tcgaaggctg gaccgatatg ctgcctgaat ttggcggtga ctcttatacc aatgcagaca
    10081 actttatgac tggtcgtgcc aacggcgtcg cgacttatcg taatactgat ttcttcggtc
    10141 tggtaaatgg tctgaacttc gcggtgcagt atcaaggtaa caacgaaggt gccagtaatg
    10201 gtcaggaagg caccaacaac ggacgtgatg ttcgccatga aaatggtgac ggctggggtc
    10261 tttccacaac atatgattta ggcatgggct ttagcgctgg tgcggcatac acctcatctg
    10321 accgcaccaa tgaccaggtt aaccatactg cggcgggtgg tgataaagca gacgcgtgga
    10381 ctgctgggct aaaatacgat gctaacaata tttacctggc aaccatgtat tcagaaacgc
    10441 gtaatatgac cccgtttggc gacagcgatt atgctgttgc aaacaaaacc cagaactttg
    10501 aagtcactgc acagtaccag tttgattttg gtctgcgtcc ggcagtctct ttcctgatgt
    10561 ctaaaggccg tgacctgcac gctgccgatg gtgcagacaa cccggcaggt gttgatgata
    10621 aagatcttgt taaatacgcc gatgttggcg cgacttacta tttcaataaa aacatgtcca
    10681 cttatgttga ctataaaatc aacctgttgg atgaagatga cagtttctac gctgccaacg
    10741 gcatctctac cgatgatatt gtcgctttag gtctggttta tcagttctaa atcctcctgc
    10801 ccgctgttat ggcgggcttt ttctgcttat tcttcctcct ttgatctaaa ttaaaaatgt
    10861 gaactccgtc attacacaaa aagtgtcatc tggcgttaca ctttatgcgg atactaaaac
    10921 agaaggtttt atgaacagaa cgattcttgt ccctatcgat atttccgatt cagaattaac
    10981 tcaacgcgtg attagccacg ttgaggcaga ggcaaagatt gacgatgcag aggttcattt
    11041 cctgacggta ataccttcac tgccttacta tgcctctctg ggtttagcgt attccgcaga
    11101 attaccggca atggatgacc tgaaagcgga agccaaatcg caactggaag agatcattaa
    11161 aaaatttaaa ctgccaaccg acagagtgca tgtccatgtt gaggaaggct cgcccaaaga
    11221 ccgcattctg gaattggcga agaagatccc cgctcatatg atcatcattg cttcccatcg
    11281 accggatatc accacttatc tgctcggttc caacgccgca gctgtagtgc gtcacgcaga
    11341 gtgctccgtg ctggttgtgc gctgacacta acgcccgcac attgctgcgg gctttttgat
    11401 tcatttcgca aatgtgctga cattttcccc tctaatccgt accatacacg ccacagtttt
    11461 atatcagatt tcttatgctg ggcgttccgg catgacgcat actcttctga tgccatataa
    11521 cgaattgagt cgcttttaaa tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa
    11581 cctgaacaag taagggcaaa aatcacaact atctgaccca taagtatttt atttattttt
    11641 caatatgtta aacattatca gcgacaacaa taagctacga taacccactc tttttttgcc
    11701 ccttttttgc cccttctaca gcgttttgcc ccatttttgc catcaacaat tttcctcata
    11761 aagtcatttc aaagcctaat agctgctcta cttttttcct caatatcatg tcgccagcag
    11821 gaaacaacct aatccctgtt ttaggcctca aagctgttaa tgataatcat tttcattttg
    11881 tatgggttat gctataaaac atagccgttg ctatgtttgt tctaattttg ctcactatag
    11941 gtactaaatt atgcactcaa taaaaaaagt aaccatgctc ttggggtgcc tcgccctcac
    12001 ctgctcgatc gcatttcagg caagtgcaac tgaaaaattc aaggtcatta caacatttac
    12061 catcatcgcg gatatggcca aaaacgtggc tggagatgct gcagaagtct catccataac
    12121 caagcctggt gcagaaattc atgagtatca gcctacccct ggcgatatta aacgtgcgca
    12181 gggggcacaa ctgattctcg ccaatggtat gaatctggaa ttgtggttcc aacgctttta
    12241 ccagcatctc aatggggttc cagaagtaat tgtctcttcg ggtgtgacgc cagtagggat
    12301 caccgaagga ccctatgagg gcaaacctaa tccccatgca tggatgtcgc cagataatgc
    12361 tctgatttac gtcgataata ttcgtgatgc gttgataaaa tacgatccgg caaatgcaca
    12421 aacctaccaa cgtaatgccg atacttataa agccaagatt acccaaaccc ttgcccccct
    12481 gcgtaagcag attacggaac tccctgagaa tcagcgatgg atggtcacca gtgaaggggc
    12541 tttttcttat ctcgcacgcg atttggggct aaaagagctt tatctgtggc cgattaatgc
    12601 cgatcaacaa ggaacaccgc agcaggtacg taaggttgtt gatatagtta agaaaaatca
    12661 tatcccggca gtctttagcg agagtacgat ttccgataaa ccagcgcgtc aggttgcgcg
    12721 tgaaaccggc gcacactacg gcggtgtgct ctatgtcgat tccctgagca cagaaaacgg
    12781 cccggtacct acgtatatcg accttcttaa ggttactacc agtacgctgg tacagggaat
    12841 taaagccggg aaaagggaga aataatgatg caatctgcag gcattgtcgt taatgatgtt
    12901 actgtcacct ggcgtaacgg gcacacagca ctgcgcgatg catccttcac agtaccaagt
    12961 ggatctattg ccgctctggt tggggtaaac ggttccggaa aatcgacgtt gtttaaagcg
    13021 attatgggat ttgtgcgtct gaccagcgga aagatatctg ttctgggtat tcccacgcga
    13081 caggcgctac agaaaaacct ggttgcctac gtccctcagt cagaagaggt tgactggtca
    13141 tttcctgtac tggtggaaga tgtggtgatg atgggccgct atgggcatat gggtatgttg
    13201 cgtatagcca aaaagaggga tcgtcagatt gtcaccgatg cgctggaacg tgtcgatatg
    13261 gtggattttc gccatcgtca aatcggcgag ctttccggtg gacaaaagaa acgcgtcttt
    13321 ctggcgagag cgattgcaca gcagggtgat gtgatcctgc tggatgaacc ttttaccggc
    13381 gttgatgtga aaaccgaagc gaaaatcatc agcctgttga gggagttacg cgcggaaggc
    13441 aaaacgatgc ttgtctcaac gcacaatctc ggctcagtca cgacattttg cgattatacg
    13501 gtcatggtca aaggcaccgt cctggcaagc gggccgacag acaccacttt tacagccgag
    13561 aacctggagc tggcttttag cggcgtactg cgccatgtca cacttaacga ctcggaagaa
    13621 agcattatta ctgacgatga acgccctttt gtggcacatc gaccgtcagc ggtgcagagg
    13681 gaagcaagat gaacgtgcta ctggaaccct tcagctatga atatatgctc aatgcgatgt
    13741 gggtctcggc aatggtcggc ggtctctgcg ctttcctgtc atgctatctg atgctaaaag
    13801 gctggtcgct gattggcgat gcgctgtcgc actctattgt cccgggtgtt gcgggggcat
    13861 atatgctggg gcttccgttt tctctgggag ccttcttttc cggcggactg gcagcgggca
    13921 gcatgctgtt tcttaaccaa cgcacccgct taaaagaaga tgccatcatc ggcctgatct
    13981 tctcgtcctt ttttggcctg gggctgttta tggtgtcatt gaacccgaca tccgtgaaca
    14041 ttcagaccat cgtcctgggc aatattctgg caatcgatcc ggcggatatc ctgcaactca
    14101 ctatcattgg cattctctcg atcatcgtgc tttttttcaa atggaaagat cttatggtga
    14161 cctttttcga cgagaatcac gcccgtgcta ttggattaca tccgggcaga ttaaaaatcc
    14221 tcttcttcac cctgctgtcg gtttcgaccg ttgcggcgct gcaaacggtt ggtgcctttc
    14281 tggtgatctg tctggtggtg actcccggtg caacagcatg gttgttaacc gatcgttttc
    14341 cccgcctgct cataattgct gtaactattg gcagtgtaac cagtttcctc ggcgcgtggg
    14401 tcagttactt tctggacggc gcgacaggtg gaatcatcgt ggtggcgcag acactgctgt
    14461 ttcttttagc atttgtattt gcgcccaccc atggactttt ggcaaatcgt cggcgtgcgc
    14521 ataaagcgct ggaggatcgc tcatgatggc attattactg gagccactgc agttcacctt
    14581 tatgagtcac gcactgctga tttcactggt agtttccata ccctgtgcgc tgctgtcggt
    14641 ctttctggtc ctgaaaggtt gggcactgat gggtgacgcg atgagtcatg cggtttttcc
    14701 tggcatcgtg cttacctgga ttctggggtt acctctggct acaggggcat tcgtcgccgg
    14761 agtattctgc gcggtggcga cgggatacct gaaagacaac agccgaatca agcaagatac
    14821 agttatgggg attgtttttt ctggcatgtt tgccgcaggt ctgatcttgt atattgcaat
    14881 caaaccagac gtacatcttg accatattct tttcggcgac atgctcggga taaccatcgg
    14941 cgatataatc cagacggtga ttattgccgg gctggttaca cttgttatta gcgcaaaatg
    15001 gcgggatttt ttgctgttca gctttgatta tcaacaggcg caggtaagcg gtttgcatac
    15061 gagatggctg cattatgggc tgctgtgcat ggtttccctg accattgtgg cgacgctgaa
    15121 agcagtaggt atcattctct ctatttctct gctcattgcg cctggcgcca ttgcagtact
    15181 cctgacacaa cgctttcata ttgcgctcct actggcgact ggcatatcgg taatagtgtc
    15241 aatgactggt gtctggctgt ccttttttat cgacagcgca ccagctccga cgattgtcgt
    15301 cttattcgcg gtcgtgttca tcatgacgtt taccgttacc agcatcaacg cacgcacaaa
    15361 gggaaacgcc catacacagg atctgttatc acccaattag cacaacatcc gaggacagca
    15421 tcgtccgcca gcctacagaa gtccggaaag ccatggaagg tcgttgggag gaggtacgaa
    15481 ttctatcatg caaaaaatac gtaaaatcga taacgcctag aaatcattca atactcgcac
    15541 tatcgaaagt ttaccagcca accgcggcac gttcctgcat acgacgtgcc gcagcttctg
    15601 ttattccacg ataaaattcc taaccgaatt caaatccttc agactattca gctcttcctt
    15661 cagctcccgc tgacggcgat aaatctcgtc attgcgctca acctgtgcct gagccattgc
    15721 tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat cggcatcacc
    15781 ccacgtcatc acgtcccgta caatgtcgga tttcgcagcc attaccaccg gataaaggcg
    15841 gcccagtgag ttggggccag cattccagat acgaccgttc cattcaaacg tgaacggctt
    15901 cgcctcctgt tctgtgcgcc atgcttcaat t
//
LOCUS       DAZPEP010000094        15511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZPEP010000094 DAZPEP010000000
VERSION     DAZPEP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.094"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            202..474
                     /gene="asr"
                     /locus_tag="ACHUT9_002804"
     CDS             202..474
                     /gene="asr"
                     /locus_tag="ACHUT9_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFR1349232.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            567..650
                     /locus_tag="ACHUT9_002805"
     CDS             567..650
                     /locus_tag="ACHUT9_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349233.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            750..1571
                     /locus_tag="ACHUT9_002806"
     CDS             750..1571
                     /locus_tag="ACHUT9_002806"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFR1349234.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUT9_002807"
     CDS             complement(1610..1939)
                     /gene="mdtI"
                     /locus_tag="ACHUT9_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFR1349235.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUT9_002808"
     CDS             complement(1926..2291)
                     /gene="mdtJ"
                     /locus_tag="ACHUT9_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFR1349236.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUT9_002809"
     CDS             2703..3737
                     /gene="tqsA"
                     /locus_tag="ACHUT9_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFR1349237.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUT9_002810"
     CDS             complement(3762..5150)
                     /gene="pntB"
                     /locus_tag="ACHUT9_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFR1349238.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUT9_002811"
     CDS             complement(5161..6693)
                     /gene="pntA"
                     /locus_tag="ACHUT9_002811"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFR1349239.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUT9_002812"
     CDS             7217..8161
                     /gene="ydgH"
                     /locus_tag="ACHUT9_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFR1349240.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8347..9729
                     /locus_tag="ACHUT9_002813"
     CDS             8347..9729
                     /locus_tag="ACHUT9_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1349241.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9766..10488
                     /gene="folM"
                     /locus_tag="ACHUT9_002814"
     CDS             9766..10488
                     /gene="folM"
                     /locus_tag="ACHUT9_002814"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFR1349242.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10485..10820)
                     /locus_tag="ACHUT9_002815"
     CDS             complement(10485..10820)
                     /locus_tag="ACHUT9_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFR1349243.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUT9_002816"
     CDS             10949..11668
                     /gene="rstA"
                     /locus_tag="ACHUT9_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFR1349244.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUT9_002817"
     CDS             11672..12973
                     /gene="rstB"
                     /locus_tag="ACHUT9_002817"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFR1349245.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            13049..13978
                     /gene="tus"
                     /locus_tag="ACHUT9_002818"
     CDS             13049..13978
                     /gene="tus"
                     /locus_tag="ACHUT9_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFR1349246.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUT9_002819"
     CDS             complement(13975..15378)
                     /gene="fumC"
                     /locus_tag="ACHUT9_002819"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFR1349247.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         3812 a         4021 c         3793 g         3885 t
ORIGIN      
        1 ggttatcgct cgccacggga atatctgcgg cagcgggctt gtaatgggtt aagtgataac
       61 agatgtctgg aaatataggg gcaaatccac tgccattccc agggatatag ttatttcaac
      121 ggccccgcgg tggggttaaa tgaaaaaaca aattgagggt atgacaatga aaaagtatta
      181 gctctggttg ttgccgctgc tatgggtctg tcttctgccg cctttgctgc agagactgcg
      241 accacacctg ctccgactgc gacgaccacc aaagcagcgc cggcgaaaac gacacatcat
      301 aaaaaacagc ataaagcagc acctgcccag aaagcgcagg cggctaaaaa gcatcataaa
      361 aatacgaaag ctgaacagaa agcccctgaa caaaaagcgc aggcagcgaa gaaacacgcc
      421 gggaaacaca gccatcagca accggcaaaa cctgctgcac aacccgcagc gtaagttttc
      481 aacagtaatg ctggcgcgcc ccctcgcgcc tgaaaattac ggtgctaagc gggtaacgtt
      541 tagcaccgcc tttagcggag ggtagtatgg tgggccgtta tcgctttgag ttcattctta
      601 tcatccttat tttatgcgca ctgattgccg cccgttttta tctttcctga ctgtagttat
      661 ctgattttac tcccactttc atcccgtccc gtctatagta tttatgaggg tttgctttta
      721 ataatcataa ttacccacca gagtgtgata tgcgtacaac cattgctgta gtgttgggtg
      781 caattagttt gacgtctgct tttgtgtttg cagataaacc agacgttgcc aaatcggcaa
      841 acgatgaggt cagcaccctg ttttttggtc atgatgatcg tgtgccagtg aatgacacga
      901 cccaatcacc gtgggatgcg gttgggcaac tggaaacggc cagcggcaat ttatgtacgg
      961 cgacgctgat tgcacccaat ctggcattaa cggcaggaca ctgtttattg acacctccaa
     1021 agggtaaagc cgataaagca gtggcgctgc gttttgtgtc aaataaaggt ctttggcgct
     1081 atgagatcca cgacatagaa ggccgcgttg atccgacact gggaaagcgg ttaaaagcag
     1141 atggggatgg ttggattgta cctcccgcag ccgcgccgtg ggacttcgga ttgattgtgc
     1201 tacgtaatcc cccttctggc attacaccgt tgccgttatt tgagggagat aaagccgcgc
     1261 ttactgccgc attaaaatcg gctggccgta aagtaactca ggcgggatac cctgaagatc
     1321 atctcgatac gttgtacagt catcaaaact gtgaagtgac tggctgggcg caaacgtcgg
     1381 tgatgtcaca tcagtgcgat accttgccgg gtgacagcgg ttcgcctctg atgttgcata
     1441 ccaatgacgg ctggcaatta attggggtgc aaagttcggc tcctgccgcg aaagatcgct
     1501 ggcgcgccga taaccgggcc atttctgtta ccggttttcg cgacaagctg gatcaactgt
     1561 cgcaaaaata atgttcaaac gctgcccgac tgtgcgggca gcgtgttcat caggcaagtt
     1621 tcaccatgat cattccagcc aacagcaaga ccaggccaat ccagccttta cgatttaacc
     1681 gttgaccaaa caagatccaa cctgcggcta acgtggcggc aataccaaac ccgccccaca
     1741 atgcataagc gacagacaag tcgatccctt taacggcctg agaaagcgca ctaaaggcag
     1801 ccagcaccgc cgccagggag agcaagccaa atattttgcg acgaaagccg tcagaaaatt
     1861 tcaaaaagac gttagcaacg atttccagca cgattgccaa tgccagccag gcggcgtgaa
     1921 cccattcaaa ctgcgccatg gttcacctcc agttcaggtt tacgcgcttt acgggtacct
     1981 gattttatca acacaatccc ggcgaccagg gtggttaacc cggcaatttt catcagcgat
     2041 aaactttcgt cgaataacaa aacgctaaac aaggtaataa ataaaatacc gataccttcc
     2101 cacagcgcat aagctacgcc taaggcgatc tttttaacgg cgaaagagag aaatatatac
     2161 gacagagaaa tcatcaccag cattaaaata aagccgccat ttccctcact gacgctcgcc
     2221 catttcattg acagcgtacc ggtaatttct gtagcaatag ccaggcctaa taaaatccaa
     2281 taaatataca ttgtccttct cctgcaagag aattatttta attttcgctt aattcagcga
     2341 aaccaaagta aagtggtcaa gccccgcgca tagggcaagc ttaggcagaa gaaaggacta
     2401 aagcgcgttg cgccagtgct gctcacctac gagcaagata gtggatgagg tacgaagatg
     2461 ggtaaatgtt gaaaataacg tcctgaacaa attgtccata atattacaat tatccgcagt
     2521 gttgcttctc gtcatcgcgg atgataattg tcctcggtag ttgaacacgc ctgatttgta
     2581 tcatagctta agaattaact caaaatattt tcacttcttt acctgagcgg tttgattttc
     2641 gttatgatga cggagcgaaa aagacattat tattagcaaa ggaagaaaaa acggggacaa
     2701 gcatggcaaa gccgatcatc acgctcaatg gcctaaaaat cgtcattatg ttgggaatgc
     2761 tggtcattat tctctgcggt atccgttttg ccgccgagat catcgtgccg tttattctcg
     2821 cattatttat tgctgttatt cttaacccgc tggtgcaaca catggtccgc tggcgagtgc
     2881 cgcgtgtact ggcggtgtcg attttgatga ccatcatcgt gatggcgatg gtgttgctat
     2941 tagcttatct gggttccgcg ctcaacgagt tgacgcggac gttaccgcaa tatcgcaact
     3001 ctattatgac gccgctgcaa gctcttgagc cgttgttgca acgcgtaggg attgacgtct
     3061 cagttgacca gctggcgcat tacattgatc cgaacgcggc gatgacgttg ctcaccaact
     3121 tattgacgca gttatctaat gccatgtcat caatattttt attgctgctg acggtgctgt
     3181 ttatgctgct cgaagtgcca caattgcccg gaaaatttca gcaaatgatg gcgcgtccgg
     3241 ttgaagggat ggcggcgatt caacgtgcaa ttgacagtgt ttctcattat ctggtgctga
     3301 aaacagccat cagcatcatc accggcctgg tcgcctgggc gatgctcgcc gcactcgatg
     3361 ttcgcttcgc ttttgtctgg ggattgctgg cctttgcgct taattacatc cctaatattg
     3421 gttcagttct cgcggcaatc ccccctatcg ctcaggtact ggtgtttaat ggcttctacg
     3481 aagcgttgct ggtgctggcg ggatatctgc tgattaatct ggtcttcggc aatattctgg
     3541 agccgcgcat catgggacgt gggctggggc tttccacatt ggtggtattt ttgtcgttga
     3601 ttttttgggg atggttgtta ggaccggtgg gtatgctgct ttccgtgccg ttaacaatta
     3661 ttgtcaaaat tgcgcttgaa caaacagcgg gaggtcaaag catcgccgtt ctgttaagcg
     3721 atctcaataa agagtgacgg cctccgcaga ggccgtcagg gttacagagc tttcaggatt
     3781 gcatccacgc tggctttggc gtcaccaaac agcatgtggg tgttttcctt gaagaacagc
     3841 gggttttgca caccagcata gccagtgttc atcgaacgtt taaagacaat cacgttctgc
     3901 gctttccaca cttccagcac aggcatacca gcaataggac tcttcggatc atcctgcgcc
     3961 gccgggttaa ccgtatcgtt agcaccaatc accagtacgg tatcggtatc agcaaagtca
     4021 tcgttgatct cgtccatttc cagcacgatg tcatacggta cttttgcttc agccagcaat
     4081 acgttcatat gtccaggcaa acgccccgca accgggtgga taccgaaacg cacgttgata
     4141 ccacgagcgc gcagtttctc ggtaatttca gcgacaggat attgcgcctg cgcgactgcc
     4201 atgccgtacc ccggagtaat gatcactgaa tgggagtttt tcagcagttc cgctgtctct
     4261 tctgcggtga tttcgcggtg ctcgcccact tcctgatcat cgccagtaga agagccgtcg
     4321 gtgccgaaac cacccgcaat aacgctgata aaggaacggt tcatcgcctt acacataatg
     4381 taagaaagga tagcccccga agaaccgacc agcgcaccgg tcacaatcag caggtcgttg
     4441 ctgagcataa agcccgcagc cgcagccgcc cagccggagt acgagttcag catcgacacc
     4501 accactggca tatctgcacc accgatggag gcgactaaat gccagccgaa taccagcgca
     4561 atggcggtca ttattagcaa tgccagcact tgcaggccga cgctgtccgt gcgaacaaat
     4621 acaatcagca gcaggaagga aacgaccaga gccgccaggt tcattttgtg acggtttggc
     4681 agcatcaacg gtttagacga aatcttgcca cacagtttgc cgaacgccac caccgaaccc
     4741 gtgaacgtta ccgccccgat gaagataccg aggaacactt ccgtcaggtg aatattgacc
     4801 agaatcggtg ccattcccgc gtcatgatgc agatagctgt taaagccaac cagcactgcc
     4861 gccagaccca cgaagctgtg caggatcgcc accagttctg gcatttcggt catttcaact
     4921 ttcttcgcca gacggatacc aattgcccca ccaatgacca tcgccagcaa gatccagcca
     4981 acattacccg tatccggtcc aaaaatggtt gcgattaacg caatcgccat cccggcgata
     5041 ccgaagttgt taccctggcg agacgtttca tgtttcgaaa gaccggccag actgaagata
     5101 aacaggatcg cggcaacaat gtatgcagct gtaactaatc ctccagacat atgttacccc
     5161 ttaatttttg cggaacattt tcagcatgcg ctgagtcacg gtgaagccac cgaaaatatt
     5221 aatgctggct ataagcaccg cgataaaact aaggaagcta acccagccgc cctggccaat
     5281 ctgcaacagt gctccgacaa caataatccc tgaaatcgcg ttggtgaccg acatcaacgg
     5341 tgtatgcagc gcgtgcgata cattccacac cacgtaataa ccgacaacgc aggccagcgc
     5401 gaaaacggtg aagtgcccaa gaaactcttt cggcgcaacg cttgccagcc aaccgaaaag
     5461 gatgattgcc agcgccatca acgcgtattt acgccacggt gagcaggcac atttttcctc
     5521 agttttcact tccggtgccg ctttttgtgc cgcctgcggc tgagctgata cctgaatcgg
     5581 cggtgccggc caggtaattt cgcccgcacg gatcacggtc acgccgcgaa tcaccacatc
     5641 atcaaaatca acagtgatat tgccgtcttt ctctttgcac aacagtttca gcagattaac
     5701 gaggttagta ccgtaaagct gcgaggattg cgtcggcaga cggcccggaa gatcggtata
     5761 accaatcact ttgacaccat tttccgtagt gaagatttca cccggcacgg tgtattcaca
     5821 gttgccaccg ttttgggctg ccaggtcgac aatcacactg cccgccttca tggagtcaac
     5881 catttcacgg gtaattagct tcggcgctgg tttgcctgga ataagcgcgg tggtgacaat
     5941 gatatcgacc tcttttgcct gggcggcaaa gagttccatt tccgctttaa tgaacgcgtc
     6001 cgacatcact ttggcatagc catcgccgct gcccgcttcc tctttaaaat ccagctcgag
     6061 gaattccgcg cccatacttt gaacttgttc tttcacttcc ggacgggtgt cgaatgcacg
     6121 cacaatcgcg ccgagactgt ttgctgcgcc aatggcggcc agacctgcaa cacccgcacc
     6181 aatcaccatc acttttgccg gtggcacttt cccggccgca gtaatttgcc cggtaaagaa
     6241 gcgcccaaat tcatgtgccg cttcaacaat ggcgcgataa ccggcgatgt tcgccatcga
     6301 gcttagtgcg tccagcgatt gtgcgcgtga gatacgcggc acagaatcca tcgccatcac
     6361 ggtcacgtta cgttccgcaa gtttttgcat taattccgga ttctgcgcag gccagataaa
     6421 actcaccagc gttgtcccag gattcagtaa cgcaatttca tcatctaacg gcgcattgac
     6481 cttcagaatg atctctgact gccagacgct attcccttct acaatttcag cgcccgcttg
     6541 cacaaacgct ttatcgtcaa aacttgccag ttgacccgcg ccgctctcta ccgcgacggt
     6601 aaaacccagt ttcagcagct gttccactgt ttttggcgtt gctgcaacac gggtttcatt
     6661 ggttaaccgt tctcttggta tgccaattcg catgatattc ccttccatcg gttttattga
     6721 tgatggtttg cctgtgtcag gagccacaca agccgctcat gtacgagcta aatgttactc
     6781 cgttaaaata aattagtaac aaacgcccta taacgtactg aaaattatgc ctgtgatcta
     6841 gcgccaaaaa tcagtatttc ggcgtgaact cgcaaaatat taacgattca gccgtgatag
     6901 tgggataaac accttagaac gccggataaa gactgataat tgtcttcgac ggtcgggtaa
     6961 aacgagacat cgccccggca cgattcacta cttaacatta aattaactta tacaattcag
     7021 ttgcttcagt agtaatgatg ctgatacggc tgttttttaa gcatagacgg tcatttgagc
     7081 aggattaaaa ttggcttaag gaatgtgata tgaaaaatga cgcagacagt tacaccgttt
     7141 aaatgcaata atcagccacg tttctcgtta ataacaatac cagtacctgg tttgcgcaag
     7201 gcgaaggatt atttttatga agcttaagaa caccctcctg gcgtcggcac tgctttctgc
     7261 tacggcattc tccgttaacg cagcaacaga actgacaccg gagcaagcgg cagcggttaa
     7321 accttttgac cgtgtagtgg ttaccggtcg ttttaatgct attggcgaag cggtgaaagc
     7381 cgtttctcgt cgcgcagata aagaaggtgc cgcctctttt tatgttgtcg acacttctga
     7441 ttttggtaac agcggtaact ggcgtgtggt cgctgacctc tataaagccg atgctgaaaa
     7501 agcagaagaa acaagtaatc gcgtaattaa cggtgttgtc gaactgccga aagatcaggc
     7561 tgttctgatt gaaccgtttg acacggtcac cgtccagggc ttctatcgta gccagccaga
     7621 agtcaatgat gccatcacca aagcggcaaa agcgaaaggt gcctactctt tctacatcgt
     7681 tcgtcaaatc gatgccaacc aaggcggcaa ccagcgtatt actgcattca tctataaaaa
     7741 agatgctaag aaacgtatcg tccagagccc ggatgtgatc ccggcagatt ccgaagcagg
     7801 acgtgcagct ctggctgccg gtggcgaagc cgcgaagaaa gttgagatcc taggtgttgc
     7861 gactaccgca tcaccaagtt ctgaagtcgg tcgtttcttt gaaacccagt catcaaaagg
     7921 cgggcgttac accgtcatgc tcccggatgg cactaaagtc gaagaactga acaaagcgac
     7981 cgcagcgatg atggtcccgt tcgacagcat caaattctct ggcaactacg gcaacatgac
     8041 cgaagtctcc tatcaggttg cgaaacgtgc cgcgaagaaa ggtgctaagt attaccacat
     8101 cacccgccag tggcaggaac gtggtaataa cctgaccgtc agcgcagatc tgtataaata
     8161 actattcagt tactcctata aggcggcttg atgccgcctt tcgcattttt agtcacaaat
     8221 ttccccgcga ccattgcatg cagacacatc tcttcgtaaa atcccgcgct tcagtggcaa
     8281 actccatttt tatgcgtttt gccctaatat ttattcttct tatcacgttt taatcactgg
     8341 atatcgatgg aaaagaaact gggactgagc gcactcaccg cgctggtatt aagctcaatg
     8401 ctgggcgcgg gtgttttcag tctgccgcaa aatatggcgg cagttgccag cccggcagca
     8461 ctgctcatcg gctggggtat tactggcgct ggcattttat tgctggcctt tgccatgctg
     8521 atcctcacgc gcattcgtcc cgaacttgac ggcggtatct ttacctatgc ccgcgaaggg
     8581 tttggcgagc taatcggctt ttgttccgca tggggatact ggctgtgcgc agtcatcgcc
     8641 aacgtctcct atctggtgat cgttttttcc gcgttaagct tttttacgga cacgccggaa
     8701 ttgcgcctgt tcggcgatgg taatacctgg caatcgatcg tcggtgcatc agctttattg
     8761 tggattgttc actttttgat cctgcgcggg gtgcaaaccg ctgccagcat taacctggtg
     8821 gcgacactgg caaaattgtt gccgctgggt ctgtttgttg tgctggcgat gatgatgttc
     8881 aaactggata ccttcaagct cgacttcacc ggacttgcac ttggcgtacc cgtttgggaa
     8941 caggtgaaaa acaccatgct gatcaccctg tgggttttca ttggtgtgga aggtgccgtg
     9001 gttgtttctg cgcgtgcgcg taataaacgt gatgttggca aagcgacact gctggcggtt
     9061 ctctccgctc tgggcgttta cttgctggta acgctgcttt cgctgggtgt ggtggcacgt
     9121 cctgaactgg ctgaaattcg taacccgtca atggccggtc tgatggtgga aatgatggga
     9181 ccatggggcg aaatcatcat cgctgccggt ttgatcgttt ccgtttgcgg cgcgtactta
     9241 agctggacca tcatggcggc agaagttccg ttcctggctg ccactcataa agcattccca
     9301 cgcatttttg cgcgccagaa cgcacaagct gcgccatccg cctcactgtg gttgaccaac
     9361 atctgcgtgc aaatttgtct ggtactcatc tggctgaccg gttccgatta caacacgttg
     9421 ctgaccatcg cctcagaaat gattctggtg ccctatttcc tggtcggcgc gttcttgctg
     9481 aaaatcgcaa cccgtccact gcataaagcg gtaggtgtcg gtgcctgcat ttatggctta
     9541 tggttattgt acgcctctgg tccaatgcac ttgctgttat ccgttgttct gtatgcgccg
     9601 gggctgctgg ttttcctgta tgcgcgcaaa acgcatacac atgataatgt actgaaccgt
     9661 caggaaatgg tgctgattgg catgctgtta attgcctcag ttccggcgac ctggatgctg
     9721 gtgggataac gttcatccca tcgttggata gcaaaggaga taacgatggg taaagcccag
     9781 cccttgccaa tattaattac tggcggaggt cgtcgcatcg gcctcgccct cgcctggcat
     9841 ttcattaatc aaaagcaacc ggtgattgtc agctatcgga cgcactatcc agccattgat
     9901 ggactgatta aggcaggtgc gcagtgtatt caggctgatt tttcgaccaa cgacggtgtg
     9961 atggcgtttg ccgatgaagt actaaaaagc ccccatggtc tgcgtgctat tttgcataac
    10021 gccagtgcgt ggatggcgga aaaaccgggt gcgccactgg ccgacgtact ggcttgcatg
    10081 atgcagatcc acgttaatac cccatacctg ctcaaccatg cgctggaaag attactgcgt
    10141 gggcacggac acgccgccag cgatatcatt cactttaccg attatgtggt ggagcgcggt
    10201 agcgacaagc atattgcgta tgctgcaagc aaagcggcac tggataatat gacccgctcg
    10261 tttgcccgca agctggcacc ggaagtgaaa gtgaactcta ttgctccatc gctgatcctg
    10321 tttaatgaac atgatgatgc cgaatatcga caacaggcgc tgaataaatc actgatgaaa
    10381 accgcgcctg gcgagaaaga agtgatcgac ctggtcgatt acttacttac cagttgcttt
    10441 gtcaccggac gcagtttccc acttgatggc ggtcgtcatc tgcgttaatg cagctttatc
    10501 cagcatataa tcaacaccca tgcgcttatc ccccaacacg aaacagaacc aacaaaagcg
    10561 gcgggcaagc gcatcattcc ggtgaaatac cacagcgaca ccaggtaaac aaaataggga
    10621 ataatcgacc acatactaaa aatgatggtt gcgcgtaagg cttcaatgcc gcgttcgctg
    10681 gcaacaatat aatgcgcgat aagcgcaaag gtcgggaaaa gtggaatcag cccggcgata
    10741 taataatttt tcgtttttgc taaaacacca atcaacagca ctaccagcgc accgagcgcg
    10801 gctttgatta ccagccccat ttttttacct taacacttcc ataacaagtc atcagtagaa
    10861 tacctgatga aaacttgttt agaaacgatt gatagtaagt aaaaacagcg cggtgtattg
    10921 tgacgttttt atatctaccg tgaatgttat gaacactatc gtatttgtgg aagatgatgc
    10981 ggaagtcggt tcactgattg ccgcgtacct ggcaaaacat gatatgcagg ttaccgtaga
    11041 gccgcgcggc gaccaggccg aagaaaccat tttgcgagaa aatccggatt tggtgttact
    11101 cgacatcatg ctaccaggca aggacggcat gaccatttgt cgtgatttac gcgcaaagtg
    11161 gtctggaccg attgttcttc taacctctct cgatagcgat atgaaccaca tcctggcact
    11221 ggaaatgggt gcctgcgact atattctcaa aacgacgccc cctgctgttt tgctagcgcg
    11281 tttacgtttg catttgcgtc agaatgagca agccacactg accaaaggtc ttcaggaaac
    11341 gtctctgact ccctacaaag ccctgcattt cggcacgttg accatcgatc ccatcaaccg
    11401 cgtagtcacc ctggctaaca ctgaaatctc gctctcgaca gctgatttcg aattattgtg
    11461 ggaattagct acccatgccg ggcaaatcat ggaccgcgat gcattgctga aaaatttacg
    11521 cggcgtcagt tatgacggac tggatcgtag cgtggacgtg gctatttcgc ggttaagaaa
    11581 aaaactgctc gataacgccg cagaacctta tcgcattaaa actgtgcgta acaaaggcta
    11641 tctttttgcg cctcatgcat gggaataagc gatgaaaaaa ctgtttatcc agttttacct
    11701 gttattgttt gtctgcttcc ttgtgatgtc tctgctggtt gggctggtgt acaaatttac
    11761 cgccgaacgc gcgggcaaac agtcgctgga tgatttgatg aacagttcac tgtatctgat
    11821 gcgcagcgaa ttgcgtgaga tccccccaca cgactggggt aagacgctga aagagatgga
    11881 tttaaatctc tctttcgatc tgcgtgtcga gccactgagt aaataccatc ttgatgatat
    11941 ttccatgcac cgactgcgtg gcggcgaaat tgtcgccctg gacgatcagt acacgttttt
    12001 gcagcgtatc ccgcgcagcc actacgtgct ggcagttggt cctgttcctt atctttatta
    12061 tctccatcag atgcgattgc tggatatcgc cctgatcact tttattgcta tttccctcgc
    12121 ctttccggtg tttatctgga tgcgtccgca ctggcaggat atgttaaaac tggaagcagc
    12181 ggcgcaacga tttggcgatg ggcatctcaa tgaacgtatc cactttgatg agggttcgag
    12241 ctttgaacga cttggtatcg catttaacca gatggcggac aatatcaacg ccttgattgc
    12301 cagcaaaaaa cagcttattg acggtatcgc tcacgaactg cgaacaccgt tagtgcgcct
    12361 gcgttatcga ctggagatga gcgataacct gagcgccgcc gaatcccagg cgttgaatcg
    12421 tgatatcagt caacttgaag ctttaattga agagctgctg acttatgccc gactcgatcg
    12481 cccacaaaac gagcttcatc ttagcgaacc agacctgccg ttgtggctgt caacgcatct
    12541 ggcagacatt caggcagtaa cgcccgataa aacggtacgg attaaaacgc tcgcgcaagg
    12601 ccattatgcg gcgttggata tgcgcttaat ggagcgcgtg ctggataatt tgctcaataa
    12661 cgccctgcgg tactgccatt caacggttga aaccagcctg ctactgtcgg ggaatagagc
    12721 gacattaatt gttgaggatg atggcccagg gattgcccca gaaaaccgcg aacatatctt
    12781 tgaacctttt gttcgcctcg atcccagccg ggatcgctca accggcggct gcgggctggg
    12841 gctggcaatt gtccactcta tagcactggc aatgggcggt acggttaatt gtgacaccag
    12901 cgaactgggt ggtgctcgct tctcgtttag ctggccgtta tggcataaca tcccgcaatt
    12961 tacctctgcc tgacactacg cgcacgatgg tcaagtcacc accactgtgc tataaaataa
    13021 gtatgttgta actaaagtgg ttaatattat ggcgcgttac gatctcgtag accgactcaa
    13081 cactaccttt cgccagatgg aacaagagct ggctgcattt gccgctcatc ttgagcaaca
    13141 caagctattg gttgcccgcg tgttctcttt gccggaggta aaaaaagagg atgagcataa
    13201 tccgcttaat cgtattgagg taaaacaaca tctcggcaac gacgcgcagt cgctggcgtt
    13261 gcgtcatttc cgccatttat ttattcaaca acagtccgaa aatcgcagca gcaaggccgc
    13321 tgtccgtctg cctggcgtgt tgtgttacca ggtcgataac ctttcgcaag cagcgttggt
    13381 cagtcatatt cagcacatca ataaactcaa gaccacgttc gagcatatcg tcacggttga
    13441 atcagaactc cccaccgcgg cacgttttga atgggtgcat cgtcatttgc cggggctgat
    13501 cacccttaat gcttaccgca cgctcaccgt tctgcacgac cccgccactt tacgctttgg
    13561 ttgggctaat aaacatatca ttaagaattt gcatcgtgat gaagtcctgg cacagctgga
    13621 aaaaagcctg aaatcaccac gcagtgtcgc accgtggacg cgcgaggagt ggcaaagaaa
    13681 actggagcga gagtatcagg atatcgctgc cctgccacag aacgcgaagt taaaaatcaa
    13741 acgtccggtg aaggtgcagc cgattgcccg cgtctggtac aaaggagatc aaaaacaagt
    13801 ccaacacgcc tgccctacac cgctgattgc actgattaat cgggataatg gcgcgggcgt
    13861 gccggacgtt ggtgagttgt taaattacga tgccgacaat gtgcagcacc gttataaacc
    13921 acaggcgcag ccgcttcgtt tgatcattcc acggctgcac ctgtatgttg cggattaacg
    13981 cccggctttc atactgccga ccatctgttc tggccgtacc cagctgtcaa actcggcttc
    14041 gctaagatac cccagcgcaa gggccgcagc ttttaaggtc agcccttctt tatgcgcttt
    14101 tttggcgatc tccgcggctt tgtcataacc aatgtgggtg ttaagcgcag tcaccagcat
    14161 cagcgattca ttgagtaatt gattgattcg ctcacgattc ggttcaatac ccactgcgca
    14221 gtgtttgtta aaactttcca tgccatctgc cagcaagcgc accgattgca ggaaattgtg
    14281 gatcaccatt ggacggaaga cgttcagttc aaagttaccg gaagcgccac ccatgttgat
    14341 cgccacgtcg ttccccatta cctgacagca gagcatggtt aatgcttcac actgggttgg
    14401 gttcactttc cccggcatga ttgagctgcc cggctcattt tccgggattg agatttcacc
    14461 aattccgcag cgcgggccag aggccagcca gcggacatca ttggcgattt tcatcagtga
    14521 cgcagccaac cctttcaacg cgccgtgcgc ctgaaccagg gcatcacagg tcgcaagcgc
    14581 ttcaaatttg ttcggcgcgg taacaaacgg tgcacaggta atgactgcca gttcatctgc
    14641 tacgcgacgc gcatactccg gatgggtatt tagtccagta cccaccgctg taccgccgag
    14701 agccagttcc gctacgtgag gcaggctgta ttcgatatgt ttgagattat gctcgagcat
    14761 cgctacccag ccggaaatct cctgccccag tgttagcggc gtggcgtcct gcaagtgggt
    14821 tcgaccgatt ttgacgatat cggcaaatgc acgcgatttt tcactcagtg tctgtgtcag
    14881 ggttttaacc tgcggaatga gttgcttgcg cagcgccagc agcgccgcaa cgtgcatcgc
    14941 cgtcggaaag acatcgttgg aactttggct tttgttcacg tcgtcgttag ggtgaacttt
    15001 acgttccatc ccgcgcacgc cgccgagtaa ttcactggcc cggttagcca gcacttcgtt
    15061 catgttcatg ttactttgcg tgccggagcc ggtctgccag atagccagcg ggaattcgtc
    15121 gtcatgctgt cctgccagta cttcatccgc cgcctgccga atggcgctcg ctttctcttc
    15181 agacaacaag cctaaatctt cattaacttt tgccgctgcg cgcttggtta gcgccagcgc
    15241 atgaatcagt gaggtgggca ttttctccgt cgaaatgcgg aaatgctcca gcgagcgttg
    15301 agtttgtgcg ccccacagct tatctgccgg gacatcaatc gcccccatcg aatctttttc
    15361 gctgcgtact gtattcatgg cctgctcctc acctgattaa ttttttcttt ctgttttgcc
    15421 ttcgttaagc aacttttagc tcacttatta tttactattt gataacaaat gtttggtctt
    15481 tcgtgccatg taaaaaaacc gccccgaagg g
//
LOCUS       DAZPEP010000095        15405 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZPEP010000095 DAZPEP010000000
VERSION     DAZPEP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15405
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.095"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..452
                     /locus_tag="ACHUT9_002820"
     CDS             <1..452
                     /locus_tag="ACHUT9_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1349248.1"
                     /translation="LCMDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISL
                     PTTISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPP
                     FHEIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            668..757
                     /locus_tag="ACHUT9_002821"
                     /pseudo
     CDS             668..757
                     /locus_tag="ACHUT9_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(758..1816)
                     /gene="modC"
                     /locus_tag="ACHUT9_002822"
     CDS             complement(758..1816)
                     /gene="modC"
                     /locus_tag="ACHUT9_002822"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFR1349249.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1819..2508)
                     /gene="modB"
                     /locus_tag="ACHUT9_002823"
     CDS             complement(1819..2508)
                     /gene="modB"
                     /locus_tag="ACHUT9_002823"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFR1349250.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2508..3281)
                     /gene="modA"
                     /locus_tag="ACHUT9_002824"
     CDS             complement(2508..3281)
                     /gene="modA"
                     /locus_tag="ACHUT9_002824"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1349251.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3447..3596)
                     /gene="acrZ"
                     /locus_tag="ACHUT9_002825"
     CDS             complement(3447..3596)
                     /gene="acrZ"
                     /locus_tag="ACHUT9_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFR1349252.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3725..4513
                     /gene="modE"
                     /locus_tag="ACHUT9_002826"
     CDS             3725..4513
                     /gene="modE"
                     /locus_tag="ACHUT9_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFR1349253.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4581..6053
                     /gene="modF"
                     /locus_tag="ACHUT9_002827"
     CDS             4581..6053
                     /gene="modF"
                     /locus_tag="ACHUT9_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFR1349254.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6314..7330
                     /gene="galE"
                     /locus_tag="ACHUT9_002828"
     CDS             6314..7330
                     /gene="galE"
                     /locus_tag="ACHUT9_002828"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFR1349255.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7340..8386
                     /gene="galT"
                     /locus_tag="ACHUT9_002829"
     CDS             7340..8386
                     /gene="galT"
                     /locus_tag="ACHUT9_002829"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFR1349256.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8390..9538
                     /gene="galK"
                     /locus_tag="ACHUT9_002830"
     CDS             8390..9538
                     /gene="galK"
                     /locus_tag="ACHUT9_002830"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFR1349257.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9532..10572
                     /gene="galM"
                     /locus_tag="ACHUT9_002831"
     CDS             9532..10572
                     /gene="galM"
                     /locus_tag="ACHUT9_002831"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFR1349258.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10775..11527
                     /gene="gpmA"
                     /locus_tag="ACHUT9_002832"
     CDS             10775..11527
                     /gene="gpmA"
                     /locus_tag="ACHUT9_002832"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFR1349259.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11686..12738)
                     /gene="aroG"
                     /locus_tag="ACHUT9_002833"
     CDS             complement(11686..12738)
                     /gene="aroG"
                     /locus_tag="ACHUT9_002833"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFR1349260.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13054..13434
                     /locus_tag="ACHUT9_002834"
     CDS             13054..13434
                     /locus_tag="ACHUT9_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFR1349261.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13548..14489
                     /gene="zitB"
                     /locus_tag="ACHUT9_002835"
     CDS             13548..14489
                     /gene="zitB"
                     /locus_tag="ACHUT9_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFR1349262.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14486..15205)
                     /gene="pnuC"
                     /locus_tag="ACHUT9_002836"
     CDS             complement(14486..15205)
                     /gene="pnuC"
                     /locus_tag="ACHUT9_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFR1349263.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15243..>15405)
                     /locus_tag="ACHUT9_002837"
     CDS             complement(15243..>15405)
                     /locus_tag="ACHUT9_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="3 different subfamilies; catalyzes the formation of
                     quinolinate from iminoaspartate and dihydroxyacetone
                     phosphate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFR1349264.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3916 a         4043 c         3850 g         3596 t
ORIGIN      
        1 gcctttgcat ggatcttgca ctggtaacag gacagcgcga aggagatctg gcggctatga
       61 gatgggagga tataagagat ggcaggctat acgtggaaca acaaaaaaca ggggcaaaaa
      121 ttcgcatttc tctccccacc acgatttcca gattgaattt aacacttgcc gatgtactgg
      181 ataacttgaa aaaaataaac ggtaaaaacg aaaagttact cggagggaag acagccagga
      241 caatcgcagc acagttcagg attgcccgag acacatccgg attgaaatgg gaaggtgacc
      301 caccgccatt tcatgaaatc cgaagtttat ccggtcgcct gcactcagca gaaaaaggcg
      361 gtgatttcac tcaggcgctc cttggacatc gttcatccag catgacggat aaataccgtg
      421 acggacgagg gagggaatgg aaagacattt agaattaatt tcgaatgatt tcgaaacacc
      481 acttgtaatt aattgattat aaatgattaa ttcctaatgg gcatttgctt catgaatgct
      541 cctttgcatt tagcaactga ctgtaacagc taaaattagt cgcttttggc ggtgaatgct
      601 gaattaaaac gcagaatttt gtccgatagt ggtatcggtg taccattcgc gcagacgaaa
      661 attcttaaat gacgcggtaa aagcgcgcgc caacattgtg attggtgata acaccaccga
      721 cagcattgcc cagttcattt atagccacct gatttaatca ggcggttatc gacacacttt
      781 taatttgcgc gtacagccac aggccaggtt tgatcgccag ttcatccctg gcccacgggc
      841 tgatacgcgc ccacagcgtt ttaccgccga cttccagctc cacttccacc tggccgttgt
      901 cgtcataact attaacaact tttgcccgca atacgttacg aatgctggtt tgctgcggcg
      961 gttgtaaaac cagagaaaca tcggaagcct gaatgcggat acgtagcgca gcttgcagcg
     1021 gttcgtccag cttattgacc cacaaatgct gatcgcccag cgccagcgcg gtcatcgcgt
     1081 aatgcggatg atgctcaagt accgtcactt tcagaatgct actttgttgc tctttcggca
     1141 gccacggatt catcacgcta ctgccccaca cttcctccag cgcgccaaag gctttcacct
     1201 gaccgttttc cagtaccatc actctgtctg ccagatggag gatctcatcc agcgaatggc
     1261 tgacatacaa catcggaatg ttgatttccc gtgtcagccg ttgcagataa ggcaacagtt
     1321 cgcgtttacg cggaatatcc agtgacgcca gcggttcatc cagcaacagc aattccggtg
     1381 ctgtcagcaa agcccgacca atcgccacgc gctgtttttc gcctccggac aggctgcctg
     1441 gtaaacggtc aagcaacggt tcaatgccta aaagcgccac cagcttatcg aactgatcga
     1501 ccatactttt cgacatgccg tagcgcagat tgccacgcac tttgtaatgc gggaacagcc
     1561 gcgcatcctg aaaaacatag ccaacgcgac gcttttccgg cgtcaggcag ataccttttt
     1621 cggcatcatt tagtacccgc ccattgagga caatccgccc tttttgcggg cgcgtcagtc
     1681 cactgatggc gttaatcagc gaagtttttc cggcaccgga gacgccaaag atagcagtga
     1741 tgccattggc gggcagtgtt tcattaatgg tcaggcaatg gttgcccaac gtctgggaaa
     1801 aattcagctc cagcatgatt agcgccccgc ccgttcacgg ctgattctgg ccagccattc
     1861 tgaaatcaac agggagatca tcgccagcgc aatagaaata atgcacagtc tcgccgcgcc
     1921 actttccccg ccgggggtct ggatcagggt atacatggca gagggaatgg ttcgcgtttc
     1981 gccaggaatg ttcgacacaa aggtgatggt tgcaccaaac tcaccgagag aacgagcaaa
     2041 agccagtacc gtaccaacaa taattcccgg taaggtcagc ggtaacgtga tagtaaagaa
     2101 aacgcgccag cgcccggcac ctagtgttct tgcggcctgt tcgagtttga catcaacccc
     2161 ttccagcgcc agacgaattg cccgcaccat cagcggaaac gacatgacgg cggcagcgag
     2221 aaccgcgccg cgccagctaa aggcgaaggt aataccaaac cagtcataca gacgttcacc
     2281 gataaatccg cgccgtccca tcgaaactaa taataagtaa ccgacgacca cgggcggtaa
     2341 caccagcggt agatgcagta cgctgtcgag cagagctttg cccggaaacg tgcaacgcac
     2401 cagtaaccag gcaaaaaaga tcccaaacgg caggctaaac agcacagcca gggaagaaac
     2461 tttcaggctt aataaaactg cctgccattc tggatcggtc agtatcatta cttggttgta
     2521 aatccgtaac gtttaaagat ttccgctgcc tgcggtccct tcagataatc ataaaatgct
     2581 ttcactgtcg cattgttatg cccttccaca acagcaaccg gatattccac ttttttatgt
     2641 gaatcttccg ggaaggtggc aaccactttt acccctttgc tgacaactgc gtcagaaccg
     2701 taaacaatgc ccaacggcgc ttcattgcgt tcgaccaacg ccagcgcgcc acgaacatct
     2761 tccgccggag ccagtttcgg ggagagcgta tcccatgcgc ccagtttttg cagtgcttct
     2821 tttgcataaa tgccagcagg gacatgttcc ggatcgccaa ccgcgaggcg accgccattc
     2881 agcagcgaag tccagttagt tgtgctgtcg atgatgaaat ctttctgctc gctggctttc
     2941 ggtgctacaa cgaccaggct attgccgagc agtgtctggc gcgaagccgt atcgatcgct
     3001 tttttatcaa ccgcataatc catccatttc tgatcggcag agataaacag atccgcaggc
     3061 gctcccgctt caatctgacg ggccagagta gatgacgaag cgaaagaaga aaccacatcc
     3121 acgccttttt cttttttata ctgcgtagca atgtcctgca ttgcgttagt cagtgatgcg
     3181 gcggcgaaca ccgtgatttt cccttcatct gccagtgtat tgccagcaac agcgaaagag
     3241 agtgccgccc cggcaaacaa gttcaaccat ttacgagcca ttggtaaccc cttaatgtaa
     3301 cgttatatag gtgacaatat aacggctagc tcagaatttt cccagcagtt attgctaacc
     3361 tttgtgaggt agataagaaa aaatatcggc tggaaaagca ggaagttgag aaaaagaaat
     3421 gcccgactaa gcgggcattc agggaatcaa tgattttgtc cgggctggtc ttttttacca
     3481 acaccagaaa agatgttgaa tacttcacca agaccgtaaa tcagacccag gatgatggcc
     3541 atcacgacag gtaccatgat tacggcgaat accagacttt ttaataactc taacatggtc
     3601 aactccagat atagtcatga gactattcta accgctaagc acagaaaagc actccccttt
     3661 tgtgcggtca gctttgcgtg gcgttcgttt tccgtcacaa taagactttt gccaggacat
     3721 tgttatgcag gccgaaatcc ttctcaccct taagctccaa caaaaattat tcgccgaccc
     3781 gcgccggatt tcgctactaa aacacattgc gctttccggt tccattagcc agggagcgaa
     3841 agatgccggt atcagctata aaagcgcctg ggatgccatt aacgagatga atcagttaag
     3901 cgagcatatt ctggttgagc gcgcaacagg cggtaaaggt ggcggcggcg cagtactgac
     3961 ccgctatggt cagcgactga ttcagctcta tgacttactg gcgcaaatcc agcaaaaagc
     4021 ctttgatgtg ttaagtgacg atgacgccct gccgctgaac agcctgctgg ccgcgatctc
     4081 acgtttttca ctgcaaacca gcgcccgtaa ccagtggttc ggtaccatca ccgcccgcga
     4141 tcatgatgac attcaacagc atgttgatgt cttactggct gacggaaaaa cacgcctgaa
     4201 agtcgcaatt accgcacaaa gcggcgcacg tctggggctg gatgaaggca aagaagtgtt
     4261 gatattgcta aaagcgccgt gggtaggcat tactcaggac gaagcggtcg cacaaaacgc
     4321 cgacaaccaa ttaccgggta ttattagtca tattgagcgc ggcgcagagc agtgcgaagt
     4381 attaatggcg ctacccgacg ggcaaacact gtgcgccaca gtgccggtaa atgaagcgac
     4441 ttctcttcag caagggcaga atgtcacggc ctactttaat gccgacagcg tgattatcgc
     4501 cacgctgtgc taagcgtgtt gacaatttgt tatgaaacac gtatccctgt cagtaatcgc
     4561 tgcacaaaat gggatataaa atgtcatcgt tgcaaatttt gcaaggcacg tttcgtctta
     4621 gcgacacaaa aacgctgcaa ttgcctcagc taacgttaaa cgcgggtgat agttgggcgt
     4681 ttgtcggttc gaatggaagc gggaaatcgg ccctggcccg cgcgctggcg ggggaacttc
     4741 cgcttttgaa aggtgaacgg caaagccagt tttcccacat cactcgtctc tccttcgagc
     4801 aattgcaaaa actcgtcagc gacgaatggc agcggaataa caccgatatg ctcggccctg
     4861 gcgaagatga caccggacgc actacggctg aaatcattca ggatgaagta aaggatgcac
     4921 cgcgttgcat gcaactggcg cagcagttcg gtattaccgc cctcctcgac cgacgcttta
     4981 aatacctttc cactggcgag acgcgaaaaa ccctgctgtg tcaggcgctg atgtcggagc
     5041 ctgacttgtt gattcttgat gagtcgttcg atggcctgga tgttgcctca cgtcagcagc
     5101 tggctgagcg actcgcctcg ttacatcagt ccggtattac tctggtactg gtgctcaatc
     5161 gcttcgatga gatcccggag tttgtccagt ttgctggcgt gctggcggat tgcacgttag
     5221 cggaaactgg cgctaaagag gaactgctcc aacaagcact cgtcgcgcaa ctggcgcata
     5281 gtgaacagct tgaaggtgtg caactgccgg agccggatga accttcagca cgtcacgcct
     5341 tacccgccaa cgaaccgcgc attgtgctga acaatggcgt ggtttcttat aacgatcgcc
     5401 ccattcttaa taaccttagc tggcaggtga atccaggtga acactggcaa attgtcgggc
     5461 caaatggtgc aggaaaatcg acgttattaa gcctggttac tggcgatcat ccgcaaggtt
     5521 acagcaacga tttgacgctt ttcggacgac gtcgcggcag cggcgaaacc atctgggata
     5581 tcaaaaagca tatcggttac gtcagcagta gtttgcatct ggattaccgg gtcagcacta
     5641 ccgtgcgtaa tgtgattctt tctggctatt ttgattcgat tggcatttat caggccgttt
     5701 cggatcgcca gcaaaaactg gtgcagcagt ggctggatat tctcggcatt gataaacgta
     5761 cggctgacgc tccgttccat agtctttcct ggggacagca gcgtctggcg ctgatcgtcc
     5821 gcgcactggt gaaacatccg acgttgctta ttctcgatga accactacag gggcttgatc
     5881 cgctcaatcg ccagcttatc cgccgttttg ttgatgtgct gattagcgaa ggtgaaacgc
     5941 aattgttatt tgtttcgcac cacgctgaag atgcgcctgc ctgtattacc catcgccttg
     6001 agttcgtgcc ggacggtgga ctctatcgct atgtgctgac aaaaatatat tgagtcggta
     6061 gtgctgacct tgccggaggc ggcctaagcg ccctctccgg ccaacggttc gacgcatgca
     6121 ggcatgaaac cgcttctttt ttcagataaa aagcgcaatc attcataaac cctctgtttt
     6181 ataatcactt aatcgcgcat aaaaaacggc taaattcttg tgtaaacgat tccactaatt
     6241 tattccatgt cacacttttc gcatctttgt tatgctatgg ttatttcata ccataagcct
     6301 aatggagcga attatgagag ttctggttac cggtggtagc ggttacattg gaagtcatac
     6361 ctgcgtgcaa ttactgcaaa acggtcatga tgtcatcatt cttgataacc tctgtaacag
     6421 taagcgcagc gtactgcctg ttatcgagcg cttaggcggc aaacatccaa cgtttgttga
     6481 aggcgatatt cgtaacgaag cgttgatgac cgagatactg cacgatcacg ctatcgacac
     6541 cgtgatccac ttcgccgggc tgaaagccgt cggcgaatcg gtacaaaaac cgctggaata
     6601 ttacgacaac aatgtcaacg gcactctgcg cctgattagc gccatgcgcg ccgctaacgt
     6661 caaaaacttt atttttagct cctccgccac cgtttatggc gatcagccca aaattccata
     6721 cgttgaaagc ttcccgaccg gcacaccgca aagcccttat ggcaaaagca aattgatggt
     6781 ggaacagatc ctcaccgacc tgcaaaaagc ccagccggac tggagcattg ccctgctgcg
     6841 ctacttcaac ccggttggcg cgcatccgtc gggcgatatg ggcgaagatc cgcaaggcat
     6901 tccgaataac ctgatgccat acatcgccca ggttgctgta ggccgtcgcg actcgctggc
     6961 gatttttggt aacgattatc cgaccgaaga tggtactggc gtacgcgatt acatccacgt
     7021 aatggatctg gcggacggtc acgtcgtggc gatggaaaaa ctggcgaaca agccaggcgt
     7081 acacatctac aacctcggtg ctggcgtagg cagcagcgtg ctggacgtgg ttaatgcctt
     7141 cagcaaagcc tgcggcaaac cggttaacta tcattttgca ccgcgtcgcg aaggcgacct
     7201 tccggcctac tgggcggacg ccagcaaagc cgaccgtgaa ctgaactggc gcgtaacgcg
     7261 cacactcgat gaaatggcgc aggacacctg gcactggcag tcacgccatc cacagggata
     7321 tcccgattaa ggaacgacca tgacgcaatt taatcccgtt gatcatccac atcgccgcta
     7381 caacccgctc accgggcaat ggattctggt ttcaccgcac cgcgctaagc gcccctggca
     7441 gggggcgcag gaaacgccag ccaaacaggt gttacctgcg cacgatccag attgcttcct
     7501 ctgcgcaggt aatgtgcggg tgacaggcga taaaaacccc gattacaccg ggacttacgt
     7561 tttcactaat gactttgcgg ccttgatgtc tgacacgcca gatgcgccag aaagcaacga
     7621 tcctttgatg cgttgccaga gcgcgcgcgg taccagccgg gtgatctgct tttcaccgga
     7681 tcacagtaaa acgttgccag aactgagcgt tgcggcattg acggaaatcg tcaaaacctg
     7741 gcaggagcaa accgcagagc tgggaaaaac gtacccgtgg gtgcaggtct ttgaaaacaa
     7801 aggtgcggcg atgggctgct ctaacccgca tccgcacgga cagatttggg caaatagctt
     7861 cctgcctaac gaagctgagc gcgaagaccg cctgcaaaaa gaatattttg ccgaacagaa
     7921 atcaccaatg ctggtggatt atgttcagcg cgagctggca gacggtagcc gtaccgttgt
     7981 cgaaaccgaa cactggttag ccgttgtgcc ttactgggct gcctggccgt tcgaaacgct
     8041 actgctgccc aaagcccacg ttttgcggat caccgatttg accgacgccc agcgcagcga
     8101 tttggcgctg gcgttgaaaa agctgaccag tcgttatgac aacctcttcc agtgctcctt
     8161 cccctactct atgggctggc acggcgcacc atttaatggc gaagagaatc aacactggca
     8221 gctgcacgcg cacttttatc cgcctctgct gcgctccgcc accgtacgta aatttatggt
     8281 tggttatgaa atgctggcag agacccagcg agacctgacc gcagaacagg cagcagagcg
     8341 tttgcgcgca gtcagcgata tccattttcg cgaatccgga gtgtaagaaa tgagtctgaa
     8401 agaaaaaaca caatctctgt ttgccaacgc atttggctac cctgccactc acaccattca
     8461 ggcgcctggc cgcgtgaatt tgattggtga acacaccgac tacaacgacg gtttcgttct
     8521 gccctgcgcg attgattatc aaaccgtgat cagctgtgca ccacgcgatg accgtaaagt
     8581 tcgcgtgatg gcagccgatt atgaaaatca gctcgacgag ttttccctca atgcgcccat
     8641 tgtcgcgcat gaaaactatc aatgggcgaa ctacgttcgt ggcgtggtga aacatctgca
     8701 actgcgtaac aacagcttcg gcggtgtgga catggtgatc agcggcaatg tgccgcaggg
     8761 tgccgggtta agttcttccg cttcactgga agtcgcggtc ggaaccgtat tgcagcagct
     8821 ttatcatctg ccgctggacg gcgcacaaat cgcgcttaac ggtcaggaag cagaaaacca
     8881 gtttgttggc tgtaactgcg ggatcatgga tcagctaatt tccgcactcg gcaagaaaga
     8941 tcatgccttg ctgattgact gccgctcact ggggactaaa gcagtttcca tgccgaaagg
     9001 tgtggctgtc gtcatcatca acagtaactt caaacgtacc ctggttggca gcgaatacaa
     9061 cacccgtcgt gaacagtgcg aaaccggtgc gcgtttcttc cagcagccag ctctgcgcga
     9121 tgtcaccatt gaagagttca acgctgttgc acatgagctg gacccaatcg tggcgaaacg
     9181 cgtgcgtcat atcctgactg aaaacgcccg caccgttgaa gctgccagcg cgctggagca
     9241 aggcgatctg aaacgtatgg gcgagttgat ggcggagtct catgcctcta tgcgcgatga
     9301 tttcgaaatc accgtgccgc aaattgacac tctggtagaa atcgtcaaag cagtgattgg
     9361 cgacaaaggt ggcgtacgca tgaccggcgg cggatttggc ggctgtatcg tcgcgctgat
     9421 cccggaagag ctggtgcctg ccgtacagca agctgtcgct gaacaatatg aagcaaaaac
     9481 aggtattaaa gagacttttt acgtttgtaa accatcacaa ggagcaggac agtgctgaac
     9541 gaaactcccg cactggcacc cgatggtcag ccgtaccgac tgttaacttt gcgtaacaac
     9601 gcagggatgg tagtcacgct gatggactgg ggtgcgactt tactttcagc tcgtattccg
     9661 ctttccgatg gcagcgtccg cgaggcgctg ctcggctgtg ccagcccgga atgctatcag
     9721 gatcaggccg cgtttctggg ggcctctatt ggtcgttatg ccaaccgcat cgccaatagc
     9781 cgttatacct ttgacggtga aacggtgacg ctttcgccaa gtcagggcgt taaccagctg
     9841 cacggcggcc cggaagggtt cgacaaacgt cgctggcaga ttgtgaacca gaacgatcgt
     9901 caggtgctgt ttgccctgag ttcagatgat ggcgatcagg gcttcccggg taatctcggc
     9961 gcgacggtgc aatatcgtct gaccgacgat aaccgtatct ccattactta tcgcgccaca
    10021 gttgataaat cttgcccggt gaatatgact aatcacgtct atttcaatct tgacggcgag
    10081 cagtctgacg tgcgcaatca caagttgcag attctggcgg gcgaatatct gccggttgat
    10141 gaaggcggca ttccgcacga cggcctgaaa tctgtcgccg gaacctcttt tgatttccgc
    10201 agcgccaaaa tcatcgccag tgagtttctt gccgacgacg atcagcgcaa agtgaaaggt
    10261 tacgatcacg cattcttgtt acaggccaaa ggcgatggca agaaagtggc ggcgcatgtc
    10321 tggtcagcag atgaaaaatt gcagctgaag gtctacacca ccgctcccgc tcttcaattc
    10381 tactccggca acttcctcgg cggcacaccg tcgcggggaa ccgaacctta cgccgactgg
    10441 caaggactgg cgttggaaag cgagtttctg ccggacagcc cgaaccaccc tgaatggccg
    10501 caaccggact gcttcctgcg tcctggcgaa gagtattcca gcctgacgga atatcagttt
    10561 attgctgagt aatgttaagc cctcgtcatg agggctttat cttcatattg ttcaaatcac
    10621 cagcaaacac cgacatattt gcaactcaat attcacaaca accttacact gcgccactat
    10681 tttcgctatg gttatgcgta agcattgctg ttgcttcgtc acggcaatat aatgagaatt
    10741 attatcataa aaagataatt tgaggagtaa gtatatggct gtaactaagc tggttctggt
    10801 tcgtcatggc gaaagtcagt ggaacaaaga aaaccgtttc accggttggt acgacgtgga
    10861 tctgtctgag aaaggcgtaa gcgaagcaaa agcagcaggt aagctgctga aagaggaagg
    10921 ttacagcttt gactttgctt acacttctgt gctgaaacgc gctatccata ccctgtggaa
    10981 tgtgctggac gaactggatc aggcatggct gcccgttgag aaatcctgga aactgaacga
    11041 acgtcactac ggtgcgttgc agggtctgaa caaagcggaa accgccgaaa agtatggcga
    11101 cgagcaggtg aaacaatggc gtcgtggttt tgccgtgact ccgccggaac tgactaaaga
    11161 tgatgagcgt tatccgggtc acgatccgcg ttacgcgaaa ctgagcgaga aagagctgcc
    11221 gctgactgaa agcctggcgc tgaccattga ccgcgtgatc ccttactgga atgaaactat
    11281 tctaccgcgt atgaagagcg gtgagcgcgt gatcatcgct gcacacggta actctttacg
    11341 tgcgctggtg aaatatcttg ataacatgag tgaagaagag attcttgagc tgaatatccc
    11401 gaccggcgtg ccgctggtgt atgagttcga cgagaatttc aaaccgctga aacgctatta
    11461 tctgggtaat gctgacgaga tcgcagcgaa agcagcggcg gttgctaacc agggtaaagc
    11521 gaagtaaacg tcattcgttt aaaaatgaga aagccgactg caagtgagtc ggcttttttg
    11581 ttgctaacaa tggagcacat tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg
    11641 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    11701 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc caatgcaggc
    11761 atcggtgatg ctcttaccgt aggccagcgg ctccccgcta tcgaggctct gattgccttc
    11821 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    11881 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    11941 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    12001 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcacc
    12061 gttaccgctg gtattcacaa tcgccgaatg cccccattta gttacggaca ggaagcagtg
    12121 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt ctgtgccatt
    12181 tttaaaacct accggacaag aaagaccaga cgccagttcg cggtgcacct gcgattccgt
    12241 ggtacgtgca ccaattgcgc cccagctcat caggtcagcg agatactgag gagtgatcat
    12301 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    12361 ggctatacgc agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcaa
    12421 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    12481 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    12541 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    12601 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagttacggg
    12661 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    12721 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    12781 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacactat gaacggatta
    12841 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    12901 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    12961 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13021 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13081 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    13141 caaactaata acggccaggc aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc
    13201 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    13261 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    13321 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaatc
    13381 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    13441 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    13501 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    13561 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    13621 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    13681 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    13741 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    13801 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    13861 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga
    13921 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    13981 gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt
    14041 tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg
    14101 ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    14161 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    14221 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    14281 ggatggtagg cgagaagccg gtgataacgc tgcatgtgca ggtgatcccg ccacacgatc
    14341 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcacg
    14401 ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg
    14461 tgtctggcca ttcacatcat caccattaat gggacagcgc gcgtgagcct ctttcacgtg
    14521 cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    14581 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    14641 cccacaacag ccagttttcc acatacttac gcgtcatcag aatcattgcc acgatagata
    14701 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    14761 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    14821 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg
    14881 ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    14941 accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15001 catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga
    15061 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    15121 ctaccgcttc gatccatgag agatcataac cgcctgcccc tattggtata tgtaccagga
    15181 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    15241 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    15301 tctcgcagcc tttcatcaag aacctcgtga ttgcttcctt cctgttctaa tgcctctgcg
    15361 atggactgaa ggccattcat ggccatccac ggacaatgcg cgcag
//
LOCUS       DAZPEP010000096        15255 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZPEP010000096 DAZPEP010000000
VERSION     DAZPEP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.096"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            247..582
                     /locus_tag="ACHUT9_002838"
     CDS             247..582
                     /locus_tag="ACHUT9_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFR1349265.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            740..1183
                     /gene="yjdN"
                     /locus_tag="ACHUT9_002839"
                     /pseudo
     CDS             740..1183
                     /gene="yjdN"
                     /locus_tag="ACHUT9_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            1316..2104
                     /gene="phnC"
                     /locus_tag="ACHUT9_002840"
     CDS             1316..2104
                     /gene="phnC"
                     /locus_tag="ACHUT9_002840"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFR1349266.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESAGIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2129..3145
                     /gene="phnD"
                     /locus_tag="ACHUT9_002841"
     CDS             2129..3145
                     /gene="phnD"
                     /locus_tag="ACHUT9_002841"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1349267.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3271..4050
                     /gene="phnE"
                     /locus_tag="ACHUT9_002842"
     CDS             3271..4050
                     /gene="phnE"
                     /locus_tag="ACHUT9_002842"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFR1349268.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4071..4796
                     /gene="phnF"
                     /locus_tag="ACHUT9_002843"
     CDS             4071..4796
                     /gene="phnF"
                     /locus_tag="ACHUT9_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFR1349269.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4797..5249
                     /gene="phnG"
                     /locus_tag="ACHUT9_002844"
     CDS             4797..5249
                     /gene="phnG"
                     /locus_tag="ACHUT9_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFR1349270.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5246..5830
                     /gene="phnH"
                     /locus_tag="ACHUT9_002845"
     CDS             5246..5830
                     /gene="phnH"
                     /locus_tag="ACHUT9_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFR1349271.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5830..6894
                     /gene="phnI"
                     /locus_tag="ACHUT9_002846"
     CDS             5830..6894
                     /gene="phnI"
                     /locus_tag="ACHUT9_002846"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFR1349272.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6887..7732
                     /gene="phnJ"
                     /locus_tag="ACHUT9_002847"
     CDS             6887..7732
                     /gene="phnJ"
                     /locus_tag="ACHUT9_002847"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFR1349273.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7729..8487
                     /gene="phnK"
                     /locus_tag="ACHUT9_002848"
     CDS             7729..8487
                     /gene="phnK"
                     /locus_tag="ACHUT9_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFR1349274.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8598..9278
                     /gene="phnL"
                     /locus_tag="ACHUT9_002849"
     CDS             8598..9278
                     /gene="phnL"
                     /locus_tag="ACHUT9_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFR1349275.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            9275..10411
                     /gene="phnM"
                     /locus_tag="ACHUT9_002850"
     CDS             9275..10411
                     /gene="phnM"
                     /locus_tag="ACHUT9_002850"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFR1349276.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10411..10968
                     /gene="phnN"
                     /locus_tag="ACHUT9_002851"
     CDS             10411..10968
                     /gene="phnN"
                     /locus_tag="ACHUT9_002851"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFR1349277.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10955..11389
                     /gene="phnO"
                     /locus_tag="ACHUT9_002852"
     CDS             10955..11389
                     /gene="phnO"
                     /locus_tag="ACHUT9_002852"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFR1349278.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11391..12149
                     /gene="phnP"
                     /locus_tag="ACHUT9_002853"
     CDS             11391..12149
                     /gene="phnP"
                     /locus_tag="ACHUT9_002853"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFR1349279.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12296..12625)
                     /gene="yjdP"
                     /locus_tag="ACHUT9_002854"
     CDS             complement(12296..12625)
                     /gene="yjdP"
                     /locus_tag="ACHUT9_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFR1349280.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12694..13011)
                     /locus_tag="ACHUT9_002855"
     CDS             complement(12694..13011)
                     /locus_tag="ACHUT9_002855"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFR1349281.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13264..15249
                     /gene="yjcS"
                     /locus_tag="ACHUT9_002856"
     CDS             13264..15249
                     /gene="yjcS"
                     /locus_tag="ACHUT9_002856"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFR1349282.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3470 a         4487 c         4198 g         3100 t
ORIGIN      
        1 cgtcagaaag ataaaaatat catgtgaata aaaaaaagaa caagtagagc attaacatta
       61 tcttaaataa taaatagagg caaaaagatt attttctttt tgcgtttcct ttcaaatgaa
      121 aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact cagggatttt gtgtataatt
      181 gcggcctttt tcggcaatct gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa
      241 atccacatgt cattaccaca ctgcccaaaa tgcaactccg aatacactta cgaagataac
      301 ggcatgtaca tctgcccgga atgtgcctac gaatggaacg acgcagaacc tgcacaggaa
      361 agcgacgagc tgatcgttaa agatgctaac ggcaatctgc tggctgacgg cgacagcgtt
      421 accatcatta aagatctgaa ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa
      481 gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg attgcaaaat cgacggtttt
      541 ggtccgatga aactgaaatc tgagtttgtg aaaaagaact gattgtattg tgatcggtaa
      601 gccggataag gcgctcgcgc cgcatccggc aacgatgccg actgcctgat gcgacgcttg
      661 cgcgtcttat caggcagcct ctcatctgta taaatttcga actacactta attggcttct
      721 cttaactgag gtcaccatca tgccgttaag tccctacctc tcttttgccg gtaactgtgc
      781 cgacgcaatt gcttattatc aacgtacgtt gggcgctgaa ctgctctata aaatcagctt
      841 cggcgaaatg ccaaaatcag cgcaggacag cgccgagaac tgcccttccg gaatgcaatt
      901 tcccgatacc gccatcgccc acgccaatgt gcgcattgcc ggaagcgaca tcatgatgag
      961 cgatgccatg ccgtcaggaa aagccagcta ctccggcttt acgctggtgc tcgattcgca
     1021 acaggtcgaa gaaggaaaac gctggtttga caatcttgcc gctaacggaa aaatcgaaat
     1081 ggcctggcag gaaactttct aggcgcatgg ctttggcaaa gtcaccgata aatttggcgt
     1141 accgtggatg attaatgtcg tcaaacaaca accaacgcaa taacccgccg ggaggcccgc
     1201 cctcccgcac tgtcatcgaa ttcccgttaa ctcttcatct gttagtcact tttaattaac
     1261 caaatcgtca caataatccg ccacgatgga gccacttttt tagggaggct gcatcatgca
     1321 aacgattatc cgtgtcgaga agctcgccaa aaccttcaat cagcatcagg cgctgcatgc
     1381 ggttgatctg aacattcatc acggtgaaat ggtggctctg cttgggccgt cgggttccgg
     1441 caaatccacc cttttacgtc acttaagcgg tttgattacc ggcgataaat ccgccggcag
     1501 ccatatcgag ctgctgggcc gcacagtcca gcgcgaaggc cgtctggcgc gcgatatccg
     1561 caaaagccgc gccaacaccg gctacatctt ccaacaattc aacctggtga accgcctgag
     1621 cgtactggag aacgtgctga ttggcgcact cggcagcacg ccgttctggc gcacctgttt
     1681 tagctggttt acccgcgagc agaaacaacg cgcgttacag gcgctgaccc gcgttggcat
     1741 ggtgcatttt gcccatcaac gcgtttccac cctctccggc ggacagcagc agcgtgtggc
     1801 gattgcccgc gcgctgatgc agcaggcgaa ggtgattctg gccgatgaac ccatcgcctc
     1861 gctggacccg gaatccgccg gcatcgtgat ggacaccctg cgcgacatca atcagaacga
     1921 cggcatcacc gtggtcgtca cgctgcatca ggtggattac gccctgcgct actgcgaacg
     1981 catcgtcgcc ctgcgccagg ggcacgtttt ctacgacggc agcagccaac agtttgataa
     2041 cgaacgtttt gaccatctct accgcagcat taatcgcatc gaagagaacg cgaaagctgc
     2101 ctgacatccc catcattgag gaaaacgaat gaacgctaag ataattgcct cgctggcctt
     2161 caccagcatg ttcagcctca gcaccctgtt aagcccggca cacgccgaag agcaggaaaa
     2221 ggcgctgaat ttcggcatta tttcaacgga atcacagcaa aacctgaaac cgcaatggac
     2281 gccattctta caggatatgg agaagaagct gggcgtgaag gtgaacgcct tctttgcccc
     2341 agactacgca ggcattatcc agggaatgcg cttcaataaa gtggatatcg cctggtacgg
     2401 caacctgtcg gcaatggaag cggtggatcg cgccaacggc caggtcttcg cccagacggt
     2461 cgcggcggat ggatcgccag gttactggag cgtgttgatc gtcaacaaag atagtccgat
     2521 caacaacctg aacgatctgc tggcgaagcg gaaagatctc accttcggca atggcgatcc
     2581 taactccacc tctggcttcc tcgtccccgg ttactacgtc tttgccaaaa acaatatctc
     2641 cgccagcgac tttaagcgca ccgtcaacgc cgggcatgaa accaacgcgc tggccgtcgc
     2701 caacaagcag gtggatgttg ccaccaacaa caccgaaaac ctcgacaagc tgaaaacctc
     2761 cgcgccagag aagctgaaag aactgaaggt gatctggaag tcgccgctga tcccaggcga
     2821 tccgatcgtc tggcgcaaga atctctccga aaccaccaaa gacaagatct acgacttctt
     2881 tatgaactac ggaaaaacgc cggaagagaa agcggtgctg gaacgcctgg gctgggcacc
     2941 gtttcgcgcc tccagcgacc tgcaactggt gccgattcgc cagctcgcac tgtttaaaga
     3001 gattcagggc gtaaaaagca ataacggact gaatgagcag gacaagctgg cgaaaaccac
     3061 cgagattcag gcgcagctgg atgacctgga ccgcctgaac aacgcgttaa gcgcgatgag
     3121 ttcggtgagt aaagcggtgc agtaatgtgc tcgatcggtc ccctctccct tgagggagag
     3181 ggttagggtg agggggaaat gcgcacgctg atgccctcac cccggccctc tcccacaggg
     3241 agagggagaa aacattaaac ggagccaaac atgcaaacca tcaccatcgc cccacccaag
     3301 cgcagctggt tctcgcttct gagctgggcc attgttctcg ccgtgttggt cgtctcgtgg
     3361 cagggcgcgg aaatggcccc gcttacgctg atcaaagacg gcggcaacat ggcgaccttc
     3421 gccgccgact tcttcccgcc cgatttcagc cagtggcagg attacctcac cgaaatggcc
     3481 gtcacgctgc aaatcgccgt ctggggcacc gcgctggcag tggttctctc catccccttc
     3541 ggcctgatga gcgccgaaaa cctggtgccg tggtgggttt accagcccgt tcgccgcctg
     3601 atggacgcct gccgcgccat taacgaaatg gtcttcgcca tgctgttcgt ggtcgccgtc
     3661 ggtctcggac cgttcgctgg cgtgctggcg ctatttattc acaccaccgg cgtgctctcg
     3721 aaactgcttt ccgaagcggt ggaagcgatt gaacccggcc cggtggaagg cattcgcgcc
     3781 accggcgcca acaagctgga agagatcctt tacggcgtgc tgccacaggt aatgccgctg
     3841 ctgatctcct actccctcta tcgcttcgaa tccaacgtcc gctcggcgac cgtcgtcggc
     3901 atggtcggtg caggcgggat cggcgtcacc ctgtgggaag cgattcgcgg tttccagttc
     3961 caacaaacct gcgccctgat ggtgcttatc atcgtcacgg tcagcctgct ggatttcctc
     4021 tctcaacggt tgcgtaagca ctttatctga taagtgaggc attgatatct atgcacttgt
     4081 ctacacatcc gaccagctac ccaacacgct atcaggagat agccgcaaaa cttgagcagg
     4141 agcttcgtca acactatcgc tgcggcgact atcttcccgc cgagcagcaa ctggcggcgc
     4201 gctttgaggt aaaccgccac accctgcgcc gcgccatcga ccaactggtg gaaaaaggct
     4261 gggtacagcg ccgtcagggc atcggcgtgc tggtgctgat gcgcccgttc gattacccgc
     4321 tcaacgccca ggcgcgtttt agccagaacc tgctggacca gggcagccat cccaccagcg
     4381 aaaaactgct gtcggtattg cgccccgcgt ccggtcacgt tgctgacgcg ctggggatta
     4441 ccgagggtga gaacgttatc catttgcgca ccctgcgtcg cgtcaacggc gtcgcgctct
     4501 gtttaatcga ccactacttt gcggacctca ccctctggcc gacactgcaa cgtttcgaca
     4561 gcggttcgct gcacgatttt ctgcgcgagc aaaccggaat tgcgctgcgc cgcagccaga
     4621 cgcggatcag cgcccgccgc gcccaggcca aagagtgcca gcgccttgaa atcccgaata
     4681 tgtcgccgct gctgtgcgtg cgcaccctta accaccgtga cggtgaaagc agcccggcgg
     4741 agtactccgt cagcctgacg cgcgccgaca tgattgaatt cactatggag cactgaatgc
     4801 acgcagatac cgcgacccgc cagcactgga tgtccgtact ggcgcacagc caaccggctg
     4861 aactggcagc ccgcctgaac gcgctgaaca tcaccgccga ctatgaagtg atccgcactg
     4921 ccgaaactgg cctggtgcag attcaggcgc gaatgggcgg taccggcgaa cgcttttttg
     4981 ccggcgacgc cacgctgacc cgtgccgccg tacgcctgac tgacggcacc ctcggctaca
     5041 gctgggtgct ggggcgtgat aaacagcacg ccgaacgctg cgcgctgatt gatgcgctga
     5101 tgcagcaatc tcgccacttt caaaacttat cagaaaccct tattgccccg ctggacgctg
     5161 accgtatggc acgcattgcc gcacgccagg ccgaagtgaa cgccagccgg gtcgacttct
     5221 ttacgatggt tcgcggagac aacgcatgac cctggaaacc gcttttatgc ttcccgtgca
     5281 ggatgcccag cacagttttc gtcgcctgtt aaaggccatg agcgagccgg gcgtgattgt
     5341 cgccctgtat cagctcaaac gcggctggca gccgctgaat atcgccacca ccagcgtgct
     5401 gctgacgctg gccgataacg acacgccggt gtggcttgcc acgccattaa gtaacgatat
     5461 cgtcaatcag agcctgcgtt ttcataccaa cgcgccgctg gtcagccagc cggaacaggc
     5521 gaccttcgcg gtgacggatg aggcgatttc cagcgaacag ctcaacgccc tttccaccga
     5581 caccgccgtt gcgccggaag cgggcgcgac gctgatttta caggtcgcca gcctgagcgg
     5641 cggacgcatg ttgcgtctca ccggcgcggg cattgccgaa gaacgaatga tcgctccgca
     5701 gctgccagag tgcattctgc acgaactcac cgagcgcccg cacccgttcc cgctcggcat
     5761 cgacctaatc ctgacctgcg gcgagcgcct gctggctatc ccgcgaacca ctcatgtgga
     5821 ggtgtgctga tgtacgttgc cgtgaaaggg ggcgaaaagg cgatcgacgc cgcccacgcc
     5881 ctgcaagaga gccgacgccg gggcgatacc gatttgcccg aactgagcgt cgcccagatt
     5941 gaacagcagc ttaacctcgc ggtggatcgc gtgatgacag aaggcggcat tgccgaccgc
     6001 gaactggcgg cgctggcgct gaaacaggcc agcggcgata acgtcgaagc gattttcctg
     6061 ctacgcgcct accgcaccac gctgacgaaa ctggcggtaa gcgagccgct cgacaccacg
     6121 gagatgcgtc tcgaacggcg tatttcagcg gtttataaag atattcccgg cggccagctg
     6181 cttggcccaa cctacgacta cacccatcgc ctgctcgatt tcactctgct ggcaaacggc
     6241 gaagcgccga cgctgaccac cgccgacagc gaacaacagc cgtcgccgca cgttttcagc
     6301 ctgctggcgc gtcaggggct ggcgaagttt gaagaggata gcggagcaca gccggatgac
     6361 atcacccgca cgccgccggt ttacccctgt tcacgttctt cccgtttgca gcagttaatg
     6421 cgcggcgacg aaggctattt gctggcgctg gcttactcca cccagcgcgg ttacgggcgc
     6481 aaccacccgt tcgcgggcga gatccgcagt ggttacatcg acgtgtcgat tgtgccggaa
     6541 gagctgggat ttgcggtaaa cgtcggcgaa ctgctgatga ccgagtgtga aatggtcaac
     6601 ggttttatcg acccgccgga tgagccgccg cacttcacgc gcggctacgg gctggtattc
     6661 ggcatgagcg agcgcaaagc gatggcaatg gcgctggttg accgtgcgtt gcaagccccg
     6721 gaatacggcg agcacgcgac aggcccggcg caggatgaag agttcgtgct ggcgcatgcc
     6781 gacaacgtcg aagccgcagg ctttgtctcg cacctcaaac tcccgcacta cgtcgatttc
     6841 caggccgaac tggagctact caaacgtctg caacaggagc agaaccatgg ctaatctgag
     6901 cggctacaac tttgcctacc tcgacgagca gaccaaacgc atgatccgcc gcgccatctt
     6961 aaaagcggtg gcgatccccg gttatcaggt accgtttggc gggcgcgaga tgccgatgcc
     7021 gtacggctgg ggaactggtg gtatccagct caccgccagc gtgattggcg aaagcgacgt
     7081 gttgaaggtg attgaccagg gtgcggatga caccaccaac gccgtgtcga ttcgcaactt
     7141 ctttaagcgc gtgaccgggg taaacaccac tgaatgtacg gacgatgcga cgcttatcca
     7201 gacgcgtcac cgcatccccg aaacgccgct gaccgaagat cagatcatta tcttccaggt
     7261 gccaatcccg gaaccgctgc gctttatcga gccgcgcgaa acggagaccc gcactatgca
     7321 cgcgctggag gagtacggcg tgatgcaggt gaaactgtat gaagatatcg cccgcttcgg
     7381 tcatatcgcc accacctatg cttatccggt gaaggtgaac gggcgctacg tgatggaccc
     7441 gtcgccgatc ccgaaattcg ataacccgaa aatggacatg atgcccgccc tgcagctgtt
     7501 cggcgcaggg cgcgagaagc gcatctatgc ggtgccgccg tttacccgcg tggaaagtct
     7561 cgatttcgac gatcacccgt tcaccgttca gcagtgggat gagccatgcg ccatctgcgg
     7621 atcgacccac agctatcttg atgaagtggt gctggatgac gccggaaacc gcatgtttgt
     7681 ctgctccgat accgattatt gccgccaaca gagcgaggcg aaaagccaat gaatcaaccg
     7741 ttactttcgg tcaataacct gacccacctt tacgcgccgg gcaaaggctt tagcgatgtc
     7801 tcttttgatt tatggccggg ggaagtgctg ggcattgtcg gggaatccgg ctccgggaag
     7861 accacgctgc tgaagtcgat ctccgcgcgt ctgacgccgc agcaggggga gattcactat
     7921 gaaaaccgtt ccctctatgc aatgagcgag gccgaccgcc gtcgcctgct gcgcaccgaa
     7981 tggggcgtgg tgcatcagca tccgctcgac ggcctgcgcc gccaggtgtc ggcaggcggc
     8041 aatatcggcg agcggctgat ggcgaccggg gcgcgacatt acggcgatat tcgcgctacc
     8101 gcgcagaagt ggctggaaga ggtggagatt cccgccaacc gtatcgacga cctgccgacc
     8161 accttttccg gcggtatgca gcagcgtttg cagattgccc gcaacctggt gacgcatccg
     8221 aagctggtgt ttatggatga accgaccggc gggctggatg tgtcggtgca ggcccgcctg
     8281 ctcgacctgc tgcgcggact ggtggtggag ctgaacctcg cggtagtgat tgtcacccac
     8341 gatttaggcg tcgcccgcct gctggcggac cgtttgctgg tgatgaagca ggggcaagtg
     8401 gtggagagtg ggttaaccga ccgcgtgctc gacgacccgc atcatccgta tacacagctg
     8461 ctggtgtcat cggttttgca gaattgagcc ggtgccggat gcggcgtaaa cgccttatcc
     8521 ggcctacaaa tgcgctcccc gtaggtcgga taagacgcgt cagcgtcgca tccgacaccc
     8581 gaaccacgag gcgaaaaatg attaacgtac aaaacgtcag taaaaccttc atcctgcacc
     8641 agcaaaacgg cgtgcgcctg cccgtcctca accgcgcctc gctcatcgtc aacgcgggcg
     8701 aatgcgtggt gctccacggc cattccggca gcggaaaatc aaccctgcta cgctcgctgt
     8761 acgccaacta tctgcccgac gaagggcaaa tccagatcaa acacggtgac gaatgggtcg
     8821 atctggtcac cgcgccagcg cgcaaagtgg tggagatccg caaaaccacc gttggctggg
     8881 tgagccagtt tctgcgcgtc atcccgcgta tttcggcgct ggaagtggtg atgcagccgc
     8941 tactcgatac cggcgtaccg cgtgaagcct gcgccgctaa agccgcgcgt ctgctcaccc
     9001 gcctgaacgt gccggaacgc ctgtggcatc tggcgccatc gaccttctcc ggcggcgaac
     9061 agcagcgcgt caacatcgcc cgcggcttta tcgtcgacta ccccattctg ctgctcgacg
     9121 aacctaccgc ctcgctggac gccaaaaaca gcgccgccgt agtcgagctg attcacgaag
     9181 ccaaagcccg tggcgcggcc atcgtcggca tcttccacga cgaagccgta cgtaatgacg
     9241 ttgccgaccg cctgcaccca atggaagcct cttcatgatt atcaataacg tcaaattggt
     9301 gctggaaaac gaagtggtac acggttcact ggaggtgcag gacggcgaaa tccgcgcctt
     9361 tgccgaaagc cagagccgcc tgccggaggc gatggacggc gaaggcggct ggctgctgcc
     9421 ggggctgatt gagctgcata ccgataatct ggataaattc ttcaccccgc gcccgaaagt
     9481 tgactggcct gcccactcgg cgatgagcag ccacgacgcg ctgatggtgg cgagcggcat
     9541 caccaccgta ctggatgccg tggcaattgg cgacgtgcgc gacggcggcg atcggctggt
     9601 gaatctggag aagatgatca acgccatcga agagacgcag aaacgcggcg tcaaccgcgc
     9661 cgagcaccgt ctgcatctgc gctgcgaact gccgcatcac accacgctgc cgctgtttga
     9721 aaaactggtg cagcgcgagc cggtgacgct ggtgtcgctg atggaccact cgccgggcca
     9781 gcgccagttc gccaatcgcg agaagtatcg cgaatattat cagggcaaat actccctcac
     9841 tgatgcgcag atgcagcagt acgaagaaga gcaactggcg ctcgccgcac gctggtcgca
     9901 gccgaatcgc gaatccatcg ccgccctgtg ccgcgcgcga aaaattgcgc ttgccagcca
     9961 cgatgacgcc acccacgccc acgttgctga atctcaccag cttggcagcg tgatcgccga
    10021 atttcccacc acgttcgaag cggcggaagc ctcgcgcaag catggcatga acgtgctgat
    10081 gggcgcgccg aatattgtgc gcggcggctc gcactccggc aacgtggcag ccagtgaact
    10141 ggcgcagctt ggcctgctgg atatcctctc ttccgactac taccccgcca gcctgctcga
    10201 tgcggcattt cgcgtcgccg atgacgagag caaccgcttt acgctgccgc aggcggtgaa
    10261 gctggtgact aaaaatccag cgcaggcgct taatctccag gatcgcgggg tgattggcga
    10321 gggcaaacgc gcggacttgg tgctggcgca tcgcaagggc aatcacattc atatcgacca
    10381 cgtctggcgt cagggtaaaa gggtgttctg atgatgggaa aactgatttg gttaatgggg
    10441 ccgtccggct ccgggaaaga tagcctgctg gcggaactcc gcctgcggga acaaactcag
    10501 ctactggtgg cgcatcgcta catcacgcgc gacgccagcg ccggaagcga aaaccatatc
    10561 gctctgagcg agcaggagtt ttttacccgc gcggggcaga acctactggc cttaagctgg
    10621 cacgctaacg gtctgtatta tggcgtcggc gtcgagatcg acctctggct gcacgccgga
    10681 ttcgacgtgc tggtcaacgg ctcacgcgcc catctgccgc aggcgcgggc gcgctatcaa
    10741 tcggcgctgc tgcccgtctg tttacaggtt tcgccggaga tcctccgcca gcgcctggaa
    10801 aaccgtggcc gtgaaaacgc cagtgaaatt aacgcccgcc tggcgcgcgc cgcccgctat
    10861 actccacagg attgccatac gctcaacaat gacggcagcc tgcgccagtc ggtcgacacg
    10921 ctgctgacgc tgattcatca gaaggagaaa caccatgcct gcttgtgagc ttcgcccggc
    10981 cacgcagtat gataccgacg cggtttacgc gctgatttgt gagctaaaac aggcggagtt
    11041 tgaccaccac gcatttcgcg tgggctttag cgccaatctg cgcgacccga acatgcgcta
    11101 tcatctggcg ctgctggatg gcgaggttgt cggcatgatc ggcctgcatt tgcagtttca
    11161 tctgcatcat gtcaactgga tcggcgaaat tcaggaactg gtagtgatgc cgcaggcgcg
    11221 cggcctgaac gtcggtagca agttgctggc gtgggcagaa gaagaagccc gccaggccgg
    11281 ggccgaaatg accgagcttt cgaccaacgt gaagcgccac gacgcgcacc gtttctatct
    11341 gcgagaaggc tacgagcaga gtcacttccg cttcaccaag gcgctgtaac atgagtctga
    11401 ccctcacgct caccggcacc ggcggcgcac agggcgttcc ggcatggggc tgcgagtgcg
    11461 cggcctgcga ccgagcgcgg cgctcgccgc agtatcgccg ccaaccgtgc agcggcgtag
    11521 tgaagtttaa cgaggcgatc accctgatcg acgccgggct gcacgatctc accgatcgct
    11581 ggtctcccgg atcgttccag cagtttttgc tgacgcatta tcatatggat cacgtccagg
    11641 ggctgtttcc gctgcgctgg ggcgtgggcg atccgatccc gatctacggc ccgccggatg
    11701 aacagggctg cgacgatctg tttaaacatc ccgggctgct tgatttcagc cacacggtgg
    11761 aaccgtttgt ggtgtttgat ttgcaggggt tgcaggtcac gccgctgccg cttaaccact
    11821 caaaactcac tttcggctat ttactggaaa cggcgcacag ccgggtggcg tggctgtctg
    11881 acaccgctgg cttgccggaa aaaacgctga aatttttacg caataatcag ccgcaggtaa
    11941 tggtgatgga ttgcagtcac ccgccgcgcg cggatgcacc gcgtaatcac tgtgatttaa
    12001 ataccgtgct tgcgctgaat caggttatcc gctcgccacg ggtgattctg acccatatca
    12061 gccaccagtt tgatgcgtgg ctgatggaaa acgcactacc gtcagggttt gaggtggggt
    12121 ttgatgggat ggagattggg gtggcgtgat gagagggaat gtgcgcgctg gccccctcac
    12181 cctaaccctc tccccagagg ggcgagggga ccgattgtgc tcgatattga atattgcgct
    12241 cgttttctcc ctctccccat tggggtgagg ggcgatgcct gctccatacc caacctcatc
    12301 gcccatactc atcttccatt ctccgctctt catcctccag ttgccgacgc tcctgatcaa
    12361 gctggcgctg gcgatcgtcc agctgcctgc ggcgatcttc aaactggcgg cggcggtcgt
    12421 catattgtct gcgccgatcg tcgctcactt cacgctgcca gccgtcgtcg cgcgaatctt
    12481 catagtctcg cccacggtca gggttataag cgtcattaat cgcctgctga atattaccaa
    12541 tagtatcgtc gataatatca gcctgggccg gaacggtgga cagcgtgagc agggtgaata
    12601 aaagaaatag cggaaagcgt ttcattagcc aacctcaaaa agaaactcta tccacattag
    12661 tcattactca tccatgcaag tagtggatga atctcaattt ctccgctgct ctattgccgt
    12721 aatcgcctcc acgcgttgtt gatgacgacc gccttcgtac tgtgcgccca gccacgcatc
    12781 cacaatcatt tttgccagtt cgaggccaac cactcgtgaa ccaaaagcca gtgcaacttc
    12841 actggcataa tgcggataat cagtgcgctc tgacgacctg gtgtctttat caatcacctc
    12901 actcattgac catgaataaa aggctggcac agcaacgcag aatcctgcgg tgccagcgac
    12961 atgaccgccc attgctccgg tacgtttttt aaaaagaaac accggttgca ttgataaatt
    13021 gtgctcagta tttgccgaag tacaatatat ataataaata tataaaacac ctcatattat
    13081 ttacagagat aataaattcg cgtaattttc cctcttcagc acaaatgcct aataaaatgg
    13141 atcgttcagc tcacacttcc acttattaaa gtcgcaaatt cccaattatg ataaccccag
    13201 ctttaaatga cgtattttgc atggattaca tatttacgac ataaatgata aatggagatt
    13261 tacatgaata actctcggtt attccgtttg agcagaattg ttattgcgtt aactgccgcc
    13321 agcggcatga tggtaaatac cgctaacgcg aaagaggaag cgaaagccgc cactcaatat
    13381 acccaacagg ttaatcagaa ttacgccaaa tcattaccgt ttagcgatcg tcaggatttt
    13441 gacgacgctc agcgtggatt tatcgctccg ctgctggatg aaggtattct gcgcgatgct
    13501 aatggcaaac catattatcg cggggaagat tataaatttg atatcaatgc gcccgcgccc
    13561 gaaaccgtta accctagcct gtggcgtcag tcgcagttaa acggtatttc cggcctgttt
    13621 aaagtcaccg acagaatgta ccaggttcgc ggtcaggata tctccaacat cacctttatc
    13681 gaaggtgata ccgggattat tgtcatcgac ccgctggtga cgccaccgag cgcaaaagcc
    13741 gcccttgacc tttacttcca aaatcgcccg caaaaaccga ttgttgcggt tatttatacc
    13801 cacagccata ccgaccacta tggcggcgtg aaaggcatta tctctgaagc tgatgtgaaa
    13861 tccggtaaag tgcaggttat cgcccctgct ggctttatgg acgaagccat cagcgaaaac
    13921 gtactggcgg gtaatattat gagccgccgt gcactttact cctacggcct gctgctggcg
    13981 cataaccctc agggtaacat cggcaatggt cttggcgtaa cgctggcatc gggctacccg
    14041 agcatcatcg caccgaacaa aaccatcacc aaaaccggtg agaagatgat tatcgacggc
    14101 ctggagtttg acttcctgat gaccccaggt agcgaagcac cagccgaaat gcacttctat
    14161 attccggccc tgaaagcgct gtgtaccgcc gagaacgcca cgcataccct gcacaacttc
    14221 tacactctgc gcggcgcgaa aacccgcgac accagcaagt ggaccgagta tctgaacgaa
    14281 acgctggata tgtggggtaa cgacgcggaa gtcctgttta tgccgcacac ctggccggtc
    14341 tggggcaata agcatatcaa tgattatatt ggtaaatatc gcgatactat caagtacatt
    14401 cacgaccaga ccctgcacct ggcgaaccag ggctacacca tgaatgaaat cggcgacatg
    14461 attaaactgc cgcctgcact tgccaataac tgggccagcc gtggctatta cggttctgtc
    14521 agccacaacg cccgcgcggt gtataacttc tatcttggct attacgacgg taacccggct
    14581 aacctgcatc cgtatggtca ggtggagatg ggtaaacgtt acgtgcaggc gctgggcggt
    14641 tctgcccgtg tcatcaacct ggcgcaagaa gcgaacaagc aaggtgatta ccgctggtcg
    14701 gcagaactgc tgaaacaggt gattgccgcc aacccgggtg accaggtcgc gaagaatctg
    14761 caagcgaata actttgaaca gctgggctat caggccgagt ccgccacctg gcgcggtttc
    14821 tacctgaccg gcgcgaaaga gctgcgcgaa ggggtgcata agttcagcca cggcaccatc
    14881 ggttccccgg acaccattcg cgggatgtcg gtcgaaatgc tgttcgactt tatgtccgtt
    14941 cgcctcgata gcgcgaaagc cgcgggtaaa aatatcagcc tgaacttcaa tatgagcaat
    15001 ggcgataacc tcaacctgac gctgaacgat agcgtgctta actaccgtaa aacactgcaa
    15061 tcccaagctg acgcctcttt ctacatcagc cgtgaagatc tgcacgccgt gctgaccggg
    15121 caagccaaaa tggcggatct ggtaaaagcg aagaaagcca aaattattgg caatggcgcg
    15181 aaactggaag aaattatcgc ctgtctggat aatttcgatt tgtgggtgaa tatcgtaacc
    15241 ccaaattaat gtcac
//
LOCUS       DAZPEP010000097        15169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZPEP010000097 DAZPEP010000000
VERSION     DAZPEP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.097"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUT9_002857"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="ACHUT9_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFR1349283.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUT9_002858"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="ACHUT9_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFR1349284.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUT9_002859"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="ACHUT9_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFR1349285.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUT9_002860"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="ACHUT9_002860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFR1349286.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUT9_002861"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="ACHUT9_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFR1349287.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="ACHUT9_002862"
     CDS             complement(8214..9107)
                     /locus_tag="ACHUT9_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFR1349288.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUT9_002863"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="ACHUT9_002863"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFR1349289.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUT9_002864"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="ACHUT9_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFR1349290.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUT9_002865"
     CDS             complement(10238..11470)
                     /gene="ycaQ"
                     /locus_tag="ACHUT9_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFR1349291.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUT9_002866"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="ACHUT9_002866"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFR1349292.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUT9_002867"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="ACHUT9_002867"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFR1349293.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..>15169)
                     /locus_tag="ACHUT9_002868"
     CDS             complement(14275..>15169)
                     /locus_tag="ACHUT9_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR1349294.1"
                     /translation="FSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQV
                     YMLDVGQGLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEH
                     LDHRGGLDSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGN
                     NHSCVVKVDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLI
                     QRVNGKVALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISS
                     LREQILPRWYHQWFGVPVDNG"
BASE COUNT         3559 a         4283 c         3740 g         3587 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtt atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaat
//
LOCUS       DAZPEP010000098        15069 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZPEP010000098 DAZPEP010000000
VERSION     DAZPEP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15069
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.098"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHUT9_002869"
     CDS             <1..161
                     /locus_tag="ACHUT9_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1349295.1"
                     /translation="WQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYR
                     AIYLRQES"
     gene            complement(199..1260)
                     /locus_tag="ACHUT9_002870"
     CDS             complement(199..1260)
                     /locus_tag="ACHUT9_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFR1349296.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1473..2936
                     /gene="bepA"
                     /locus_tag="ACHUT9_002871"
     CDS             1473..2936
                     /gene="bepA"
                     /locus_tag="ACHUT9_002871"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFR1349297.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLLERFKPYTKM"
     gene            2957..3316
                     /gene="arsC"
                     /locus_tag="ACHUT9_002872"
     CDS             2957..3316
                     /gene="arsC"
                     /locus_tag="ACHUT9_002872"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFR1349298.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3454..4155)
                     /locus_tag="ACHUT9_002873"
     CDS             complement(3454..4155)
                     /locus_tag="ACHUT9_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFR1349299.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHUT9_002874"
     CDS             complement(4250..5539)
                     /gene="uraA"
                     /locus_tag="ACHUT9_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFR1349300.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHUT9_002875"
     CDS             complement(5625..6251)
                     /gene="upp"
                     /locus_tag="ACHUT9_002875"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFR1349301.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6576..7613
                     /gene="purM"
                     /locus_tag="ACHUT9_002876"
     CDS             6576..7613
                     /gene="purM"
                     /locus_tag="ACHUT9_002876"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFR1349302.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7613..8251
                     /gene="purN"
                     /locus_tag="ACHUT9_002877"
     CDS             7613..8251
                     /gene="purN"
                     /locus_tag="ACHUT9_002877"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFR1349303.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHUT9_002878"
     CDS             8422..10488
                     /gene="ppk1"
                     /locus_tag="ACHUT9_002878"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFR1349304.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10493..12034
                     /gene="ppx"
                     /locus_tag="ACHUT9_002879"
     CDS             10493..12034
                     /gene="ppx"
                     /locus_tag="ACHUT9_002879"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFR1349305.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHUT9_002880"
     CDS             complement(12073..14316)
                     /gene="pdeF"
                     /locus_tag="ACHUT9_002880"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFR1349306.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14498..14650)
                     /locus_tag="ACHUT9_002881"
     CDS             complement(14498..14650)
                     /locus_tag="ACHUT9_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349307.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14668..14859
                     /locus_tag="ACHUT9_002882"
     CDS             14668..14859
                     /locus_tag="ACHUT9_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFR1349308.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         3990 a         3827 c         3705 g         3547 t
ORIGIN      
        1 tctggcaact ggcgcacagt agcgcagaca attttccggc actgacggta agccatttta
       61 ttaccgccaa tctgctcccg gtgatgctgg gtaatattat cggcggtgca gtgctggtga
      121 gtatgtgtta tcgggctatt tatttacgtc aggaatcctg aaaaatcagc ccggcgaaac
      181 agttcgtcgg gctgaagatt attcttgcgc tatttgccca tcgggccagg cgtggatcac
      241 ggctttgatc agcgtcgcca atggaatagc gaagaatacg ccccagaatc cccacaaacc
      301 accgaagatc accaccgata aaataatcac cagcggatgc aggttaaccg cttcggagaa
      361 caacaccggt actaacagat taccgtccag cgcctgaata atcagataca ctgcgaagca
      421 gctccagaat tccgtgcctg caccaaactg gaatagcgcc acgccaacca ccggaatggt
      481 caccacaaat gcgccaatgt acggaataag taccgagaag ccgaccagca ccgccagcag
      541 cagcgaatag ttcagcccaa agagcaagaa ccccagccaa gtggcgatcc ccaccacgat
      601 catctccagc actttgccgc ggatatagtt ggtgatttgt tgattcatct ccttccacac
      661 ctgtcctgcc agtccacggt tgcgcggcag cacccgacga acggcgttca gcatctgctc
      721 tttgtctttc agcaggaaga agaccatcaa tggtaccagc accagataga cggcaatggt
      781 cagcaaaccg accagcgagg cgagggaaat tttcaccacc gaatcgccca tggtcagcat
      841 ccgactgcgc atattttcgg ccattgcatc aataatgccc gcgtccatta atgccggata
      901 gcggcgcggc aacgtggcgg caaagtcaga aagcttattg agcatccccg gcatatcgcg
      961 gatcaagtag atgccctgtt gccaggcgat aggcagtacc acgaacgcca tcagtagcaa
     1021 tataccgacg aaaaccacca atacaatcga cgtcgcccag cggcgggagc agccaataga
     1081 ttgcaggcgc acggttggcc attccagcaa ataggccagc acaatagcca ccagcaacgg
     1141 agcaagcagg ccactaaaga aaaagataat gccaaatccg gcaactaaaa taaccagcaa
     1201 ggcaatcgct tccgggtcgc taaaacggcg gcgataccat tgcatcaaca tttcgagcat
     1261 aaagaccttc cctgaacctc aagagcggga ttgcgatccg caattgtatc gaaatgtcac
     1321 aaaaaagact tcgcttttta tgacggattc aggaaactga aaagtcattt gagtgggcta
     1381 atcttcgccg ttacactcaa aggcggcgcg gtgggaacaa tatttcacag tatcggtcaa
     1441 atgactatct tcagaaatac aggatagagg ttatgttcag gcagttgaaa aaaaacctgg
     1501 ttgcaaccct cattgctgct atgaccattg gtcaggtagc cccggcgttt gccgacagcg
     1561 cagacacctt gccggatatg ggaacctccg caggaagcac gctttccatt ggtcaggaaa
     1621 tgcagatggg cgactattat gtccgccagc tacgcggcag cgcgccgtta attaatgacc
     1681 cgctgttaac gcaatatatt aattcgctgg ggatgcgtct ggtttcgcat gccaattcgg
     1741 ttaagacacc gtttcatttt tttctgatca acaacgacga aattaacgcc tttgctttct
     1801 ttggcggcaa cgtggtgctg cactctgccc tgttccgtta ttccgataac gaaagtcaac
     1861 tggcttcagt tatggcgcac gaaatctccc acgtcaccca acgtcacctg gcgcgagcga
     1921 tggaagatca gcagcgcagc gcgccgctga cctgggtcgg cgcgttaggt tctattttac
     1981 tggcgatggc cagtccgcag gcggggatgg cggcgctgac cggtacactg gcgggaacgc
     2041 gtcaggggat gatcagtttc acccagcaaa atgaacagga agcggaccgc attggtattc
     2101 aggtgctgca acgctcggga ttcgatccgc aggcgatgcc aaccttcctc gaaaaattac
     2161 tcgatcaggc gcgttactcc tcgcgcccgc cggaaatttt attgactcac ccgttgccgg
     2221 aaagtcgtct ggcagatgcc cgcaaccgtg ctaatcagat gcgcccgatg gtggtgcagt
     2281 cgtcggaaga tttctatctg gcgaaagcgc gcacactggg gatgtataat tccggacgta
     2341 accagctcac cagtgatttg ctggatgaat gggcgaaagg aaacgttcgt cagcaacgag
     2401 cggcgcaata tggtcgtgct ttacaggcga tggaagccaa taaatacgac gaggcgcgta
     2461 aaacgctgca accgttactg gcggcagaac ctggcaacgc atggtatctc gatctggcta
     2521 ccgatatcga tcttgggcaa aacaaagcca atgaggcaat caatcgcctg aaaaatgccc
     2581 gtgatttgcg caccaatccg gtgttgcagc tcaacctggc aaacgcttat ctgcaaggcg
     2641 gtcaaccaca agaagcggcc aatattctga atcgctacac ctttaataat aaagatgaca
     2701 gcaacggctg ggatttactg gcacaggcgg aagccgcgct aaataaccgc gatcaggagc
     2761 tggctgcgcg agcagaaggt tatgcgctcg ccggacgact cgatcaggcc atttcgctgt
     2821 tgagtagcgc cagttcgcag gtgaaattag gcagccagca acaagcgcgt tacgatgcgc
     2881 gcatcgacca gttgcgccag ctgctggaac gctttaagcc ttataccaag atgtaattcg
     2941 cattaaagga agaacaatga ccaaacaggt aaaaatctac cataacccac gctgttcaaa
     3001 gagccgggaa acgctgaacc tgctgaaaaa aaacggcgtg gagccagaag tggtactcta
     3061 ccttgagaca cccgccgatg cggcaacgct gcgcgatttg ctgaaaatgc tggggatgaa
     3121 tagcgcccgt gaattgatgc gccagaaaga ggatctttat aaagaactga acctggctga
     3181 cagttcgctt agcgaagagg cgctgattca ggcaatggtg gataatccga agctgatgga
     3241 gcgcccgatt gtggtcgcga atggcaaagc gcggattggt cgtccaccgg agcaggtact
     3301 ggaaatcgtg ggttaagatg tgccccatcg ctagttgaag cacattgccg gatgcgacgc
     3361 ttgccatgtc ttatccggcc tacgaatact atggattaac ttcgcgtagt tcggataagg
     3421 cgttcgcgcc gcatccgaca ataaacacct tatctacaac ttcagaattt ctttcacaaa
     3481 cggaatggtc agcttacgtt gcgcggtaat cgacgcacga tccaactgat ccaacgtcat
     3541 aaatagcgtg cgcatttctc tgtcgagccg cttcagcaag aaacgcccca catcttccgg
     3601 cagttcaaaa ccacgcaaac gcgcgcgtaa ctgtagcgcc tgcaacttat cttcatcaga
     3661 aagtggctgc aatttgtaga tctgccccca gtcgagtcgc gacgcgagat ccggtaatcc
     3721 cagattcaac tgccgcggtg gacgatcgcc ggtgatcaac aatcgtgttt tgcccgattc
     3781 cagaattcga ttgtagagat cgaaaatcgc catctcccac aactcatcgc ctgcaataca
     3841 ctcaatgttg tcgatacaga ccagcgacaa atgctccata ccgtcgagca cttccggaac
     3901 aaaccaggtg cgtttatcca gcgggacata gcccaccgca tcgccacgct gcgacaattc
     3961 cgcgcaagcc gcgtgcagca gatggctgcg ccccgcgcct tcgcgtgccc agagatagat
     4021 gtaaccgcta tgttcctgac gcagcacgtt ttgcagcgca gccagtaaag aggagttatc
     4081 ccccggccag aaacttgcaa aggtttcgtc gtcaggaaga taaagtggca aagagagctg
     4141 tgccggtgtg ttcagagata cctcaaccag gatttcacaa aatcgcgaga agtttaccac
     4201 agaatcccat gatgtttgaa ccgggcagca acactgcccg gtcggtacat tatttgtctg
     4261 ttatatccgc gtcttctgcg tccagcacca cttcttcagg gcgcagcaca ctaatcagtt
     4321 tgaagatcaa acttaagccg ataccgacga tggtcgccag cgccatacct ttcagctccg
     4381 ccgcgccaat gtttaccttc gcgccactga caccgatgat caaaatcacg gaagtcagga
     4441 tcaggttctg tgctttgttg taatccactt ttgattcgat caaaacacga ataccggaag
     4501 caccgatgac accataaagc agcagcgaaa cgccgcccat caccggcaat gggatcatct
     4561 ggatagcggc agccagttta ccgacgcagg aaagcaggat agcgaaaatc gccgccccac
     4621 cgataaccca ggtactgtaa acacgggtga tcgccatcac gccaatgttt tctccgtaag
     4681 tggtatttgg cgtagagcca aagaagccgg agatcacagt cgacaggccg ttagcaaaca
     4741 tcgaacggtg cagacctgga tcgcgcagca gatctttttt gacgatatta gccgttacta
     4801 ccaggtgccc tacgtgttcg gcaataacca ctaacgccgc tggcagaata gtcagaatgg
     4861 caaaccactc gaagcgcggc gtatagaggg ttggcagcgc aaaccagtga gcattaataa
     4921 tcggcgtggt atcgacaatt cccattgcga aagagagcgc gtaccccacc agcacgccaa
     4981 ttaaaatcgg gataattgcc aggaaaccac ggaacagcac ggaacctaaa accgtgaccg
     5041 ccagggtggt aatagagatg atgatggttt tggagtctgg cgtttgccct tcagccggga
     5101 gtaaacccgc cataccggca gctacgcccg ccagctccag accgatgacg gcaacgattg
     5161 cgcccattgc cgcaggtgga aacagcacgt ccagccagcc ggtccccgct ttcttcacga
     5221 taaaagaaac caggcagaac agcacgccgc acataataaa gccgcccagc gcgacttcat
     5281 accctaacgg caacagtaac aataccggtg aaataaaggc aaagctggaa ccaagataag
     5341 ccggaatttt ccctttacag atgaagagat acagcagcgt tccaataccg ttaaataaca
     5401 gtacagtcgc cgggttaata tgaaataaga cgggcaccag gacggttgca ccaaacatgg
     5461 cgaacaaatg ttgcaaacta agcgggattg tctgtaaaag tggcggtctt tcactcaccc
     5521 cgatagcacg gcgcgtcata gtattatcct ctgtattatg tgttataggc gctttactca
     5581 aaaaaaagcc gactcttaaa gtcggcttta attattttta ttctttattt cgtaccaaag
     5641 attttgtcac cggcatcgcc gaggcccgga ataatgtatc cgtgctcgtt cagtccctgg
     5701 tcaatcgatg cggtatacag ttcgacgtcc gggtgcgctt tttccagcgc agcgatacct
     5761 tctggcgcag ctaccagcac cagaactttg atgctgctgc agcccgcttt tttcagcagg
     5821 tcgatggtcg cgataacgga accaccggtt gccagcattg ggtcaacgat cagcgccata
     5881 cgctcatcga tgttagaaac cagtttctgg aagtacggta ccggctccag cgtttcttca
     5941 ttacggtaca taccgacaac gctgatgcgc gcgctcggaa cattttccag cacaccgtcc
     6001 atcatcccca gaccggcacg cagaattggc acaacggtaa ttttcttacc tttgatctgg
     6061 tcgatttcta ccgggccgtt ccagccttcg atagttactt tttccgtttc gaggtcggcg
     6121 gtcgcttcgt aagtcagcag gctacccact tcggaagcga gttcgcgaaa gcgcttggtg
     6181 ctgatatctt gctcacgcat cagtcccagc ttgtgtttga cgagtgggtg tttgacttcc
     6241 acgatcttca tactctttct cctttgaggg gcagccacaa aaaaaatcga cggattatac
     6301 ctcctttctt caaggcggca atattctttt cgttgacttt agtcaaaatg ataacggttt
     6361 gagataaagt tattttatat tcaaatggtt atgaaagaag attattccat ccgaaaacta
     6421 acctttgccc tggcacaagt cttctttcgc cgcgcgtctg gggaaaagac gtgcaaaaag
     6481 gttgtgcaaa gcagtctcga aaacgtttgc tttccctgtt agaattgcgc cgaattttat
     6541 ttttctaccg caagtaacgc gtggggaccc aagcagtgac cgataaaacc tctcttagct
     6601 acaaagatgc cggtgttgat attgacgcgg gtaatgctct ggttggaaga atcaaaggcg
     6661 tagtgaagaa aacgcgtcgt ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt
     6721 gtgcattgcc gcaaaaatat cgtgaacccg tgctggtttc cggcactgac ggcgtaggta
     6781 ccaagctgcg tctggcaatg gacttaaaac gtcacgacac cattggtatt gatctggtcg
     6841 ccatgtgcgt taatgacctg gtggtgcaag gtgcagaacc gctgtttttc ctcgactatt
     6901 acgcaaccgg aaaactggat gttgataccg cttcagcggt gatcagcggt atcgcggaag
     6961 gatgtctgca atcgggctgt tcactggtgg gtggcgaaac ggcagaaatg ccggggatgt
     7021 atcacggtga ggattacgat gtcgcgggtt tctgcgttgg cgtggtagaa aaatcagaaa
     7081 tcatcgacgg ctctaaagtc agcgacggcg atgtgctgat tgcactcggt tccagcggtc
     7141 cgcactcgaa cggctattcg ctggtgcgca aaattcttga agtcagcggt tgtgatccgc
     7201 aaaccaccga acttgatggt aagccattag ccgatcatct gctggcaccg acccgcattt
     7261 acgtgaagtc agtgctggag ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga
     7321 ccggcggcgg cttctgggaa aacattccgc gcgtattgcc agataatact caggcagtga
     7381 ttgatgaatc ttcctggcag tggccggaag tgttcaactg gctgcaaacg gctggtaacg
     7441 ttgagcgcca tgaaatgtat cgcaccttca actgcggcgt cgggatgatt atcgccctgc
     7501 ctgctccgga agtggacaaa gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga
     7561 aaatcggtat catcaaagcc tctgattccg aacaacgcgt ggttatcgaa taatgaatat
     7621 tgtggtgctt atttccggca acggaagtaa tttacaggca attattgacg cctgtaaaac
     7681 caacaaaatt aaaggcacca tacgggcagt tttcagcaat aaggccgacg cgttcggcct
     7741 tgaacgcgcc cgccaggcgg gtattgcaac gcatacgctc atcgccagcg cgtttgacag
     7801 tcgtgaagcc tatgaccggg agttgattca tgaaatcgac atgtacgcac ccgatgtggt
     7861 cgtgctggct ggttttatgc gcattctcag cccggcgttt gtctcccact atgccgggcg
     7921 tttgctgaac attcaccctt ctctgctgcc gaaatatccc ggattacaca cccatcgtca
     7981 ggcgctggaa aatggtgatg aagagcacgg tacatcggtg catttcgtca ccgatgaact
     8041 ggacggtggc ccggttattt tacaggcgaa agtcccggta tttgctggtg atacggaaga
     8101 tgacatcacc gcccgcgtgc aaacccagga acacgctatt tatccactgg tgattagctg
     8161 gtttgccgat ggtcgtctga aaatgcacga aaacgccgcg tggctggatg gtcaacgtct
     8221 gccgccgcag ggctacgctg ccgacgagta atgccccgta gttaaagcgc cagctctgcc
     8281 gctggcgttt ttcaattcac ctgtaaatcg caagctccag cagttttttt cccccttttc
     8341 tggcatagtt ggacatctgc caatattgct cgccataata tccaggcagt gtcccgtgaa
     8401 taaaacggag taaaagtggt aatgggtcag gaaaagctat acatcgaaaa agagctcagt
     8461 tggttatcgt tcaatgaacg cgtgcttcag gaagcggcgg acaaatctaa cccgctgatt
     8521 gaaaggatgc gtttcctggg gatctattcc aataaccttg atgagttcta taaagtccgc
     8581 ttcgctgaac tgaagcgacg catcattatt agcgaagaac aaggctccaa ctctcattcc
     8641 cgccatttac tgggcaaaat tcagtcccgg gtgctgaaag ccgatcagga attcgacggc
     8701 ctctacaacg agctgctgct ggagatggcg cgtaaccaga tcttcctgat taatgaacgc
     8761 cagctctccg tcaatcaaca aaactggctg cgtcattatt ttaagcagta tctgcgtcag
     8821 cacattacgc cgattttaat caatcctgac actgacttag tgcagttcct gaaagatgat
     8881 tacacctatc tggcggtgga aattatccgt ggcgatacca tccgttacgc gctgctggag
     8941 atcccatcag ataaagtgcc gcgctttgtg aatttaccgc cagaagcacc gcgtcgacgc
     9001 aagccgatga ttcttctgga taacattctg cgttactgcc ttgatgatat tttcaaaggc
     9061 ttctttgatt atgacgcgct gaatgcctat tcaatgaaga tgacccgcga tgccgaatac
     9121 gatttagtgc atgagatgga agccagcctg atggagttga tgtcttccag tctcaagcag
     9181 cgtttaactg ctgagccggt gcgttttgtt tatcagcgcg atatgcccaa tgcgctggtt
     9241 gaagtgttac gcgaaaaact gactatttcc cgctacgact ccatcgtccc cggcggtcgt
     9301 tatcataatt ttaaagactt tattaatttc cccaatgtcg gcaaagccaa tctggtgaac
     9361 aaaccactgc cgcgtttacg ccatatttgg tttgataaag cccagttccg caatggtttt
     9421 gatgccattc gcgaacgcga tgtgttgctc tattatcctt atcacacctt tgagcatgtg
     9481 ctggaactgc tgcgtcaggc ttcgttcgac ccgagcgtac tggcgattaa aattaacatt
     9541 taccgtgtgg caaaagattc acgtatcatc gactcgatga tccacgctgc gcacaacggc
     9601 aagaaagtga ccgtggtggt tgagttacag gcgcgtttcg acgaagaagc caacattcac
     9661 tgggcgaagc gcctgaccga agcaggcgtg cacgttatct tctctgcgcc ggggctgaaa
     9721 attcacgcca aactgttcct gatttcacgt aaagaaaacg gtgaagtggt ccgttacgca
     9781 cacatcggga ccgggaactt taacgaaaaa accgcgcgtc tttatactga ctattcgttg
     9841 ctgaccgcag atgcgcgtat caccaacgaa gtacggcggg tatttaactt tattgaaaac
     9901 ccataccgcc cggtgacatt tgattattta atggtgtcac cgcaaaactc tcgccgtctg
     9961 ttatatgaaa tggtagaccg cgaaatcgcc aacgcgcagc aagggctgcc cagtggtatc
    10021 accctgaagc taaataacct tgtcgataaa ggcctggttg atcgtctgta tgcggcctcc
    10081 agctccggcg taccggttaa tctgctggtt cgcggaatgt gttcgctgat ccccaatctg
    10141 gaaggcatta gcgacaacat tcgtgccatc agtattgttg accgttacct tgaacatgac
    10201 cgggtttata tttttgaaaa tggcggcgat aaaaaggtct acctttcttc cgccgactgg
    10261 atgacgcgca atattgatta tcgtattgaa gtggcgacac cgctgctcga tccgcgcctg
    10321 aagcagcggg tgctggacat catcgacata ttgttcagcg atacggtcaa agctcgttat
    10381 atcgataaag aactcagtaa tcgctacgtt ccccgcggca atcgccgcaa agtacgggcg
    10441 cagttggcga tttatgacta catcaaatca ctcgaacaac ctgaataacc ctatgccaat
    10501 acacgataaa tcccctcgtc cgcaggagtt tgctgcggtc gatcttggtt caaacagttt
    10561 tcacatggtc atagcccgtg tggtagatgg tgccatgcag attattggcc gcctgaaaca
    10621 gcgggtgcat ctggcggacg gcctggggcc agataatatg ttgagtgaag aggcaatgac
    10681 gcgcggtttg aactgtctgt cgctgtttgc cgaacggcta caagggtttt ctcctgccag
    10741 cgtctgtata gtcggcaccc atacgctgcg tcaggcgctg aacgccactg actttctgaa
    10801 acgcgcggaa aaggtcattc cctacccgat tgaaattatt tccggtaatg aagaagcccg
    10861 tctgattttt atgggcgtgg aacataccca accggagaaa ggtcgcaaac tggttattga
    10921 tattggcggc ggatctacgg aactggtgat tggtgaaaat ttcgaaccga ttctcgttga
    10981 aagccgccgg atgggttgtg tcagctttgc ccagctttac ttccctggcg gggtcatcaa
    11041 taaagagaat tttcagcgcg ctcgtatggc ggcagcacaa aaactggaaa ctttaacctg
    11101 gcaattccgt attcagggct ggaacgtggc aatgggcgct tccggtacca taaaagccgc
    11161 ccatgaagtg ttaatggaaa tgggcgagaa agacgggata attaccccgg aacgtctgga
    11221 aaaactggta aaagaagttt tacgtcaccg taatttcgca tcgctgagtt taccgggtct
    11281 ttccgaagag cggaaaacag tcttcgttcc tggactggcg attttatgcg gtgtgtttga
    11341 tgctttagcc atccgtgaac tgcgcctttc tgacggggcg ttacgcgaag gcgtactgta
    11401 cgaaatggaa ggacgtttcc gtcatcagga tgtgcgtagt cgcaccgcca gcagcctcgc
    11461 caaccagtat cacatcgaca gcgaacaggc ccgacgggtg ctggatacca ctatgcaaat
    11521 gtacgaacag tggcgggaac agcaaccgaa gctggcgcat ccgcaactgg aggcgctact
    11581 gcgatgggcc gccatgctgc atgaggtcgg gttgaatatc aaccacagcg gtttgcatcg
    11641 ccactccgct tatattctgc aaaacagtga cttgccgggt tttaatcagg aacagcagct
    11701 gatgatggcg acactggtgc gctatcaccg taaagcgatt aaactcgacg atctgccgcg
    11761 ctttaccttg tttaagaaga aacagttcct gccactgatt cagctattgc gccttggcgt
    11821 attactcaac aatcaacgtc aggcaaccac cacaccgcca acattgacac tgattactga
    11881 tgacagtcac tggacactgc gtttcccgca tgactggttt agtcagaatg cgttggtact
    11941 gcttgatctg gaaaaggagc aagaatactg ggaaggcgtg gctggctggc ggttgaaaat
    12001 tgaagaagaa agtacacctg aaatcgccgc ttaatgcttt gcgggccgac gagaaatgtc
    12061 ggcccgcatt attcaggcac tttcgcgaat gggttcgatt tcattcaacg tatcaattaa
    12121 cggctgcggc ttaccaataa gataaccctg catataatcg atccccaaag agagcaccgc
    12181 ctcgcggatc tcttcgtttt caacgtactc tgccactacc cgcattttct tcattcgcgc
    12241 cagatggcaa atcgacgcca caatctgata atccagacta tttgacacaa tattgcggat
    12301 aaagctgccg tcaattttaa gcagatcggc attcacattt ttaagccgtg catagctggc
    12361 gtagccggtg ccgaaatcat caatcgcaat ctggcagcct aattcctgaa gatgctgcaa
    12421 ggtgatttgc gcctgcttaa cattggtcag agcattactt tcggtgactt caaaaataag
    12481 ttgccacgct tcaatctgat atttagccag caactgactg acttcaacag gaaaacgagc
    12541 ctgacatacc gaggttggag acagattaat agcaaaacgg tgagcgggca tcttcgctct
    12601 gttttcagcc ataaattgca gcgtatgctc aatgacccac atgtcgatac tcgacgataa
    12661 accaaattcg tgcgcgaccg gcaagaagct atcggggacg atcagttcat cattctcacc
    12721 tttcatgcgc agaagaattt catggtaaac atcaccacgc ataccggtaa tcggctgggc
    12781 catcaggaaa aaatggttgt gttccagcgc ctgctgtagt cgattcatca tcgcgacttt
    12841 atctttcaat tcgcgttgca aatacattgc cccgcgacgc tgcatatttt ccggggcgtt
    12901 ggtcacgatg gaaagttcgg cgaccgtatt tagctctccc agcagcaggt agatatgatt
    12961 cactggcgag cgcacatagc agtaactgac gccaatctgc ggttgcatcg gcatcccatc
    13021 ccaaaagaaa cgaaattgct tgagatgact atccagtgcg gtaatgcgct cctggtgcga
    13081 ttctgtattc agtcgcagcg cgagatcgtt acccgaaagc tgataaacat cttcacccgg
    13141 ttccagcaag ggtgacagcc agtgagaaag tttttgcttg tattgaatcc gcagcatgat
    13201 gccatagttc ttaaccagca tttccatgcc agggatgcgt aaataacaaa gcgcagacca
    13261 gggagcatca cgcaacgcgc gattcagggc gcgaacattt ggcagatgaa ccaccgggtc
    13321 catatacgca agccgctgaa tgcgtctgac taccgctctc tgacgggttg ccagtacagc
    13381 catataattg acaataaaag agaataccag ataactggag gaggttatgg tcagctgcgt
    13441 ggtatagcca ggataaatgg gaatgtaatt ttgatagctg tggatgctga tcatcaacac
    13501 gaccgcccag agcagcgaaa tcagcttata accatagcgc atcgctcccc acatcatcag
    13561 gggcagcaat aatgacaagg tataattggt gctaaaaatt gtgctttttt catttaacgg
    13621 catgcataac agcaacaata aagcacctaa tgccagtagc cagagcgcga actctttttt
    13681 ggtgactttg gcatcaacct gctgttttaa ttgcgaataa tagctacgta aataaaatgg
    13741 atttcgcact acccgaatga tgaagtagca cagcgggaca ccgatcagat tacccaccag
    13801 taaggcctga taattgatta aagtcccgag gttaaaaggc atgacaccga ccagattttc
    13861 tctgctcgcc agtaatccta caaatgcagc aaactggaaa agtatcagaa acagcgttgc
    13921 aggaaacaca atctgccaga atatacgctg ggaaattaaa cgggtatcac catgtgaaac
    13981 gttgttacgc cggggagtaa agaccctgta accgccccaa cagagaatga taatgataat
    14041 aaaattagcc gttaatgata gcgtttcgta aaaacccagc tctgcatatt tgcggacaaa
    14101 aatccctaag gcgatccctg gcaatgccgc ccagctgaaa aacatcatca tgctgatcat
    14161 cagtgccaga ggcagataaa agagaaaaac ctcaccggaa gaaatatgcg cgaaagtatt
    14221 aatgtgggcg aaaatgggca agattaaaga aggcaggaac agcggaagcc cccaccattt
    14281 atcacgcatt tttatataag ttgcattcag tttcatagat gctcagcaga atcccccaca
    14341 tcctgaagga ggtgtattca gacaggcatc ccacctgact tcgaatgatg attattcatc
    14401 actatagaga gcattgattc taagtgtcat atgaaagttc caattgatat atatcaaaca
    14461 aaataaccct gattaatgaa ttattacgtt tatcatgtta attcatcatt attacatcat
    14521 cattgtaaat aattaaatta acttccataa cattaaaata tgtatccact gacgtttttt
    14581 tacataacga agaattgacc attttgtcct gttgtgcctt aatgtaagta ccgtccacag
    14641 cgtgggacat acttcaagga accttttgtg agtcaggcaa ccagtatgcg aaaacgacac
    14701 cgatttaaca gtcgcatgac ccgtatcgta ctgctcatca gctttatctt cttctttggc
    14761 cgttttatct actcgtccgt cggtgcctgg cagcaccatc agagcaaaaa agaagctcag
    14821 caatccacac tctccgtcga atcaccggta caacgttagc ggttaccttc ttcactttca
    14881 cagaacataa cggcacttcg ctgtcggatg cttttgctct ttgggattat caaagcggca
    14941 gatattcttt catcttaaat tttacgtctt tgtcctgact gatgtttatc ctgtttggct
    15001 gcggaataaa tataaaatta atatatatgt tgtaatgata tatttttata aattattccc
    15061 tgcgtgaat
//
LOCUS       DAZPEP010000099        14886 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZPEP010000099 DAZPEP010000000
VERSION     DAZPEP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14886
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.099"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            261..1559
                     /gene="kgtP"
                     /locus_tag="ACHUT9_002883"
     CDS             261..1559
                     /gene="kgtP"
                     /locus_tag="ACHUT9_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFR1349309.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1556..1879)
                     /locus_tag="ACHUT9_002884"
     CDS             complement(1556..1879)
                     /locus_tag="ACHUT9_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFR1349310.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHUT9_002885"
     CDS             complement(1925..3280)
                     /gene="pssA"
                     /locus_tag="ACHUT9_002885"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFR1349311.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHUT9_002886"
     CDS             complement(3394..6054)
                     /gene="pat"
                     /locus_tag="ACHUT9_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFR1349312.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHUT9_002887"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6086..6784)
                     /gene="tapT"
                     /locus_tag="ACHUT9_002887"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFR1349313.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHUT9_002888"
     CDS             complement(6853..7272)
                     /gene="trxC"
                     /locus_tag="ACHUT9_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFR1349314.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7479..8516
                     /locus_tag="ACHUT9_002889"
     CDS             7479..8516
                     /locus_tag="ACHUT9_002889"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFR1349315.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHUT9_002890"
     CDS             complement(8564..9253)
                     /gene="ung"
                     /locus_tag="ACHUT9_002890"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFR1349316.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9558..9941
                     /gene="grcA"
                     /locus_tag="ACHUT9_002891"
     CDS             9558..9941
                     /gene="grcA"
                     /locus_tag="ACHUT9_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFR1349317.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHUT9_002892"
     CDS             complement(9997..10584)
                     /gene="eamB"
                     /locus_tag="ACHUT9_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFR1349318.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10687..11568
                     /locus_tag="ACHUT9_002893"
     CDS             10687..11568
                     /locus_tag="ACHUT9_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1349319.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHUT9_002894"
     CDS             complement(11777..13111)
                     /gene="srmB"
                     /locus_tag="ACHUT9_002894"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFR1349320.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13243..13980
                     /gene="trmN"
                     /locus_tag="ACHUT9_002895"
     CDS             13243..13980
                     /gene="trmN"
                     /locus_tag="ACHUT9_002895"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFR1349321.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13965..>14886)
                     /locus_tag="ACHUT9_002896"
     CDS             complement(13965..>14886)
                     /locus_tag="ACHUT9_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFR1349322.1"
                     /translation="VANLEFNQFPPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFM
                     PDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLG
                     IDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLE
                     CLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVG
                     IVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKES
                     RGLHFTLDYPELLTHSGPSILPPGNHYINR"
BASE COUNT         3589 a         3767 c         3899 g         3631 t
ORIGIN      
        1 gatgcgacac tgcgcgtctt atactcccac atatgccaga ttcagcaacg gatacggttt
       61 ccccaacttg cccacttcca tacgtgtcct ccttaccaga aatttatcct taagctcctc
      121 aataaccatt ttcctgctaa ctaaattcat ggttaaggtt gcacaatgat atgcaacaaa
      181 tgtataacat ttcctttacc aaaaaaaata aacaaaagcg accgacaaaa gcatcggatt
      241 acggcaggag acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa
      301 gtagtgatac tcgtcgccgc atttgggcaa ttgtgggggc ctcttcaggt aatctggtcg
      361 agtggttcga tttctatgtc tactcgttct gttcactcta cttcgcccac atcttcttcc
      421 cttccgggaa cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc
      481 tgatgcgccc aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa
      541 aatcgatgct gctatcggtg tgtatgatgt gtttcggctc actggtaatc gcctgtttgc
      601 ctggttatga aacaatcggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc
      661 agggattatc cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg
      721 ttgaagggcg caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac
      781 tgctagccct actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag
      841 agtggggatg gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt
      901 tacgtcgtca gttagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat
      961 ctctgaaagg attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg
     1021 cgggctccct ttgtttctat accttcacta cttatatgca gaagtatctg gtaaatactg
     1081 cgggaatgca tgtcaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc
     1141 ttattcaacc actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat
     1201 gtttcggttc gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt
     1261 cctcgcctta tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata
     1321 catcaatcag tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg
     1381 ttggcctgtc atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt
     1441 tgtcgctgaa atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg
     1501 tggtggcgtt tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt
     1561 gacgggtcag ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat
     1621 ccttccagct ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca
     1681 aactgacaga gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct
     1741 gtgaatattc atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat
     1801 cctgcccgct ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac
     1861 agacaaaaag aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt
     1921 gtgattacag gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga
     1981 gtttacgaac cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga
     2041 tggtggtatg ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agcttcagtt
     2101 gtggatcgtg gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt
     2161 tattaccggt gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt
     2221 tgtcgtcatc tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac
     2281 ggctcaggaa acgacgcaga ttgatctcat agagataagg caatgcgcca attatcttga
     2341 aaggttcatc ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga
     2401 cctttttccc ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga
     2461 agtatggcgt acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct
     2521 tgttcaacag actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt
     2581 cggcatcgcc ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat
     2641 cgttcttgat ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc
     2701 cattcataat attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga
     2761 taagatgata acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc
     2821 tggcaccgct ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa
     2881 gggcttcacg ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct
     2941 gcgccatgcg gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac
     3001 gttgtgcacg atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag
     3061 cttcatacaa cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga
     3121 caatgcaaat gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg
     3181 cgggagcgta aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa
     3241 ggtgttgttg atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg
     3301 ttcattgaag ggtcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc
     3361 ccgattaagt ggtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt
     3421 tagcgtaagc ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg
     3481 ggctagcgcc accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc
     3541 gtgatctcgc gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt
     3601 gagatccgag cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc
     3661 acgcgtgacg ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat
     3721 ttcccgatcg tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat
     3781 ctcgctaaag tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag
     3841 ttgtggctca tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc
     3901 tacccattct tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc
     3961 ttcaaacggc gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag
     4021 caaaggatga atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg
     4081 caccagaagc tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat
     4141 ctttttactt ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg
     4201 cagtgcgacg acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat
     4261 caacggcccg aaaaccggat cgtgctcaac cacaacccgc aactcctgag cgccagcacg
     4321 gttagccata ctttgcacca acaggccgtg gacccgcgcc tgtggccagg ccatttttac
     4381 gcgatcgaaa atagcgttcg ccgcttgctg gacttcattg gctgtacgca ggtaaagcat
     4441 gacgccctga acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg
     4501 ataaccaatc tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg
     4561 gagcgtgttc atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt
     4621 agccccttcg gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag
     4681 attgctgggc aacgtcggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat
     4741 atgcataaaa gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct
     4801 gaataaacgt cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac
     4861 atatttgctg cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc
     4921 gggagcagcg gcgctgggcg aatgaataac catcagcgca tcaaaatcct ggctgtggag
     4981 cagaatgtcc agcgttttga cataatgctc gctgctggcg tcatcgcgca gatcgagagg
     5041 gttagagact gccacatgtt ctggcaatgc atcgcgcaat ttctggcagg tttcttcgct
     5101 tagcgttgcc agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc
     5161 agcaccgttg ctgataatca tcaaccggtc gccacgcagc gggcgcatat ggctaagggt
     5221 ttccaccgcc gaaaacagtt cgtgggtatc ctgtacccgc aacaaaccgg cacgctgaat
     5281 agccgcatcc catgccgggt ccattcctgc cgtcgtgttg agcagtcgct gtgccgccgg
     5341 gctacgtccg cttttaatca ccagaatcgg tttattacgc gaggcactac gggctgccga
     5401 aacaaagcgt cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact
     5461 gtcgcgcgcc agatagtcga gcaattcatc aacgtcgata tccaggctgt cgccgagcgc
     5521 aataaagtag gaaaagccca tctcacgctg ttgcgcccag tcgaggatgg tgttggagac
     5581 ggctgccgat tgtgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct
     5641 ggcattcaga ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat
     5701 gttatggcgc agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat
     5761 aatgcaggtt ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc
     5821 attggtacat aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc
     5881 caacacaccc aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagct
     5941 tcccgccagc aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc
     6001 aattaccgct atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt
     6061 ctcccgcaga agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac
     6121 ccagatgttg cgtttttcct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta
     6181 accctgctgc cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag
     6241 tacaatattg gcctgcagcc tgggcttcac gcaggcgata ggcagaaagc cgggaaagat
     6301 cgacggaaat gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt
     6361 ccggccaggt accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga
     6421 tcacttcccg ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg
     6481 ggttttgtac cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa
     6541 cggtatcagg caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg
     6601 tgtcgaacat cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga
     6661 gacataattt ctctggcaaa agacagcgtt ggcagcgatg aacgcgatta ccgcgggcaa
     6721 gaaaaggacg tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gcgttttcgg
     6781 tcataagaga gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc
     6841 accccggtaa gattaaagag attcgttcag ccagctatcg aacggcgctt tcggtactgc
     6901 gccattaagc atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg
     6961 aattccaaag cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac
     7021 tttaccgcta cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc
     7081 gcaccacggt gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag
     7141 tgtttcaccg gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca
     7201 ttttgccgca tcttcgatcc gatcgtcggg aatgcgattg atggcctgac aatgggtaca
     7261 aacggtattc ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta
     7321 tgtttctaat atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc
     7381 aataggtatg atagtcgcag gctttggtac gaaatcatgg cgaaccgcgc ggacttcggg
     7441 taatctgcgc gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta
     7501 aaagcggcaa ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc
     7561 acaacccgcg taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc
     7621 gtcgccccgc ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc
     7681 cgtggcgcac ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg
     7741 gcatcagtgg taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa
     7801 cccgcgtcta cggcgaaaat gcctgtcagg cactgttcca gagccgtccg gaagcaattg
     7861 ttcgcgcctg gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg
     7921 cggcaaaccg caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca
     7981 cggaacatca cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc
     8041 agtgggtaag ccaggcaggc gcgcaggatt gcgttctggc actggaaaat gaatctaacc
     8101 cgcataacct gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg
     8161 tgcaggatgc ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag
     8221 agcacgttca gccgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg
     8281 caggttacac cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc
     8341 tgccagcgaa aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac
     8401 gcgatccgaa cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata
     8461 tctctgtcgc aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa
     8521 tcagccgggt ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg
     8581 gtaatactgg catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca
     8641 gcacaaaatg gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg
     8701 ctttcagtac atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc
     8761 cccacaacaa aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag
     8821 taaacgtctc ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca
     8881 cagtattgag cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc
     8941 gggtgaagcc cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa
     9001 tggcaatgcc gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag
     9061 gatcctggcc gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa
     9121 agacatcttt ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct
     9181 gaagggtatt aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta
     9241 attcgttagc catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt
     9301 ctacagaaca aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg
     9361 gtaaaatttc acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat
     9421 cgatgtgtgg tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata
     9481 cactcaagca acaatggttt taccaattgg ccgcgacagg ctgaacaaat caaataattt
     9541 tgccggggag gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc
     9601 tgctgaactc tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa
     9661 aagcaggtta tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc
     9721 gtgaagttcc agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg
     9781 ttaacgttct gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc
     9841 agctgaccat ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc
     9901 agcgcgacgt tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac
     9961 taagcggaag agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca
    10021 atagaccagt aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg
    10081 ctgaaacaga tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag
    10141 caaaacgctg acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga
    10201 cagtgccgta acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc
    10261 ccaaaagctg attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt
    10321 ccacgccagc cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg
    10381 gtcaatcact gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc
    10441 cagactcatc cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct
    10501 aagggcgaga atattgttcg gtcctggcgt catagcggta atcagggtgt aagtccaaaa
    10561 agcacttaaa agggtcggtg tcacttgtta tctcctctgt tttggtagac agtacccgac
    10621 acggaacatt taaaaaaatg aatgttttta tattctagat tcgatatttt cgataggttt
    10681 ggggttatgg atctgcgccg ttttattacg cttaaaaccg tggtggaaga gggttccttt
    10741 ttgcgagctt cgcaaaaatt gtgctgtaca caatcgacgg tgacttttca tattcagcag
    10801 cttgagcagg agttttcagt ccagttattt gagaaaattg gccgacggat gtgccttacc
    10861 cgcgaaggaa aaaagttgct gccgcacatt tatgaactta cccgagtgat ggatacgctt
    10921 cgtgaggctg ccaaaaagga gtcagatccg gacggggaac tacgcgttgt ttcgggcgaa
    10981 acgttgctct cttaccgaat gccgcaggtg ttgcagcgtt ttcggcaacg cgcaccaaaa
    11041 gtgcgtttat cgttgcagtc gctgaactgt tacgtgatcc gtgatgcgct actgaatgat
    11101 gaggccgatg tcggcgtctt ttatcgcgta gggaatgatg atgccctgaa ccgacgagag
    11161 ttgggtgaac aatcgctggt gctggtggct tcaccacaaa ttgtagatgt cgattttact
    11221 gagccgggaa gacataacgc ctgtagcttt attatcaatg aaccacaatg tgtcttccgg
    11281 cagatatttg agagcacgct gcgtcagcgg cggatcacgg tggaaaacac cattgagctt
    11341 ataagtatcg aaagcatcaa gcgttgtgtc gcggcgaata tcggagtcag ctacctgccg
    11401 cgttttgcgg tggcaaaaga gttggagtgc ggagaattaa ttgaattgcc ttttggcgaa
    11461 cagtcgcaga ccattacggc catgtgcgca caccatgctg gaaaagcggt tagcccggcg
    11521 atgcacactt ttattcagtg tgttgaagag agttttgtgg ctggataaaa ataatgtcgg
    11581 gattaaaccc ggcatttaac ttgtcgacaa agtgcgcttt attcatgccg gatgcggcgt
    11641 gaacgcctta tccggcctac aagatcgtgc aaattcaata tattgcatga gccacgtagg
    11701 cctgataagc gtagcgcatc aggcagtttt gcgtttgcca gcacttttaa taccaggctt
    11761 aaacccggca ttgagattac tcttctgtcg tttgcggtgg cacgcccgtt ccgcttggtt
    11821 tacggcgctt accaatattt ttggtgtcgc gatggcgttt tttcacccgc ggtttctctt
    11881 tctctttagc ttttttcttc tcggcgcgtt ttgccagtac tttcttcgat ggcttgccgg
    11941 tctgcttctc gcttggcgca cgcgttttcg ggcgtaactc atcaataacg cgagctttaa
    12001 tcggctcttc aatgtaacgg ccaaccttgc ccagcagcaa atggtcatgg gcttccacca
    12061 gcgaaattgc ggtgccttta cgaccggcgc gcgcggtacg tccgatacgg tgcaaataag
    12121 tatcgccact gcgcggcata tcgaagttaa agacgtggct gacgtcagga atgtcgatac
    12181 cgcgcgcggc aacatcggtt gcaaccagta cgtttacgcg accttcggtc aaacgcttga
    12241 tcgcttcgtt acgcttgcct tgtaccatct caccttcgag atagcagttg ttgatgcccg
    12301 cttcgcgcag ccagtttgcc agctcatgca cacgttcacg cttacgcaca aacacaattg
    12361 agcgggtcgc ttccggctgt tttaacagat gcaccagcaa tgcggtttta tgttccaggt
    12421 catcggcgcg gtaataccac tgatgaattt ttttgcgctc acgggtggac ggattggcag
    12481 aaacctccac cggatcttcc agcagacgct cggcaaagtc ctgaatcgca tcgccttcca
    12541 gcgttgccga aaagagcagg gtctgtttac gccagcgcgt ttcgccagca atatgttcga
    12601 tatcctgagc gaagcccata tccagcatac ggtctgcttc gtcgaggatc agcgtttcaa
    12661 ccgcgcggca atcgaagttc tcttctttta tgtattgcag cagacgtccg gtcgtggcga
    12721 ccacgatgtc ctgattttcg ctgaacactt ccgcgtggtt catataggct acgccgccgg
    12781 tgatggtggc gatatccaga tgcgtatgtt tcgccagttc gcgggcatga tcggccacct
    12841 gcatcgccag ctcgcgagtt ggggtgagga tcaaaatacg cggcggaccg gatttcttac
    12901 gtgggaaatc gagcaggtgc tgcaacgctg gcagcagata cgccgccgtt ttaccggtgc
    12961 ctgtcggcgc agaaccgagt acatcacggc catcgagcgc aggcggaatg gcggcagcct
    13021 gaatggcggt cgggcgagtg aaacctttat cctggagggc ttccagcagg ctttcgtcga
    13081 gttcaagttc ggaaaaagtc gttacagtca tgttctacct ctgtgtgggg cgctgattat
    13141 agacgttacg gctgcaatct tcatctgttt gtatggatat cgcttctcgg gtattgttct
    13201 cacccggtgc tatcgccgtt tttcctgcaa ggttttattt tcatgtcaca gtctacatcc
    13261 gtgcttcgtc gtaatggatt tacttttaaa cagttttttg ttgctcacga tcgctgtgcg
    13321 atgaaagtgg gaacggatgg cattttgctg ggcgcatggg caccggtggc tggggtgaaa
    13381 cgttgccttg atatcggcgc gggtagcggg ttgctggcac tgatgctggc gcagcgaacc
    13441 agtgacagcg tgataattga tgccgttgag ctggaaagtg aagctgccgc gcaggcgcag
    13501 gaaaatatca accagtcccc gtgggcagag cggattaacg tccatacggc ggatattcag
    13561 cagtggctca cacagcagac agtacgcttc gatttaatca tcagtaaccc accttactat
    13621 cagcagggag tagagtgcgc gacacctcaa cgggaacagg ctcgctatac cactacgctt
    13681 gatcacccat cgctgctgac ctgtgcggcg gagtgcatta ccgaagaggg atttttctgc
    13741 gtggtgctgc cagagcaggt tggtaatggt tttactgaac tggcattaag catgggctgg
    13801 catttacgtt tgcggacgga tgtggcggaa aacgaagcgc gactgccgca tcgggtgctg
    13861 ctggcattct ccccgcaggc gggagaatgc tttagcgatc gcttagtcat tcgtgggcca
    13921 gaccagaact attccgaagc gtatacggcg ctgacccagg ctttttatct gtttatgtaa
    13981 tgattgccgg ggggaaggat cgatggaccg gaatgcgtga gcagttccgg gtaatccagc
    14041 gtaaaatgca ggccacgact ctccttgcgc atcatcgcac agcgaacaat caactcggca
    14101 acctgtacca gattacgcag ctccagcaaa ttattcgaca cgcggaagtg ggcgtaatat
    14161 tcgtctattt cctgttgcaa catattgata cgccgcaggg cgcgctcaag acgttttgtt
    14221 gtgcgcacaa tgccgacgta atcccacata aacagccgta gctcgtgcca gttatgctga
    14281 attactaccc gttcgtcagg gttctcaacg cggctttcat cccacggtgg taacgtactg
    14341 acgccgtggg catcaggcat acgtctggtg atatcttctg ccgccgacca gccatagacc
    14401 agacactcca gcaatgaatt cgaggccatg cggttagcgc cgtgtaagcc ggtataactc
    14461 acctcgccaa tggcatacaa gccctcgacg tccgtacgcc catgatcatc aaccattaca
    14521 ccaccgcagg tatagtgtgc agcaggcaca atcggtaccg gttcttgtgt gagatcaatc
    14581 cccagcccga gcaatttttc ataaatcatc gggaaatgct ggcgaataaa atcggcgggc
    14641 ttatggctga tgtcgaggaa catacaatct gcgccgaggc gtttcatttc atggtcaatg
    14701 gcgcgggcga caatatcgcg cggggccagt tcgccgcgct catcaaaatc gggcataaaa
    14761 cgcgtgccat ccgggcgctt gagataagcg ccttcgccgc gcagtgcttc cgttaacagg
    14821 aaattgcgtg cctgtgggtg atacagtgcg gtagggggga actgattaaa ttcgagattg
    14881 gcaacc
//
LOCUS       DAZPEP010000100        14715 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZPEP010000100 DAZPEP010000000
VERSION     DAZPEP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.100"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(173..877)
                     /locus_tag="ACHUT9_002897"
     CDS             complement(173..877)
                     /locus_tag="ACHUT9_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFR1349323.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1014..1466)
                     /gene="moaE"
                     /locus_tag="ACHUT9_002898"
     CDS             complement(1014..1466)
                     /gene="moaE"
                     /locus_tag="ACHUT9_002898"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFR1349324.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1468..1713)
                     /gene="moaD"
                     /locus_tag="ACHUT9_002899"
     CDS             complement(1468..1713)
                     /gene="moaD"
                     /locus_tag="ACHUT9_002899"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFR1349325.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(1706..2191)
                     /gene="moaC"
                     /locus_tag="ACHUT9_002900"
     CDS             complement(1706..2191)
                     /gene="moaC"
                     /locus_tag="ACHUT9_002900"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFR1349326.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2194..2706)
                     /gene="moaB"
                     /locus_tag="ACHUT9_002901"
     CDS             complement(2194..2706)
                     /gene="moaB"
                     /locus_tag="ACHUT9_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFR1349327.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2728..3717)
                     /gene="moaA"
                     /locus_tag="ACHUT9_002902"
     CDS             complement(2728..3717)
                     /gene="moaA"
                     /locus_tag="ACHUT9_002902"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFR1349328.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3705..3849)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4114..5022
                     /gene="yvcK"
                     /locus_tag="ACHUT9_002903"
     CDS             4114..5022
                     /gene="yvcK"
                     /locus_tag="ACHUT9_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFR1349329.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5214..7235)
                     /gene="uvrB"
                     /locus_tag="ACHUT9_002904"
     CDS             complement(5214..7235)
                     /gene="uvrB"
                     /locus_tag="ACHUT9_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFR1349330.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(7814..8482)
                     /gene="bioD"
                     /locus_tag="ACHUT9_002905"
     CDS             complement(7814..8482)
                     /gene="bioD"
                     /locus_tag="ACHUT9_002905"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1349331.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENAATGKYIN
                     LALL"
     gene            complement(8475..9230)
                     /gene="bioC"
                     /locus_tag="ACHUT9_002906"
     CDS             complement(8475..9230)
                     /gene="bioC"
                     /locus_tag="ACHUT9_002906"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFR1349332.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9217..10371)
                     /gene="bioF"
                     /locus_tag="ACHUT9_002907"
     CDS             complement(9217..10371)
                     /gene="bioF"
                     /locus_tag="ACHUT9_002907"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFR1349333.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(10368..11408)
                     /gene="bioB"
                     /locus_tag="ACHUT9_002908"
     CDS             complement(10368..11408)
                     /gene="bioB"
                     /locus_tag="ACHUT9_002908"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFR1349334.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            11495..12784
                     /gene="bioA"
                     /locus_tag="ACHUT9_002909"
     CDS             11495..12784
                     /gene="bioA"
                     /locus_tag="ACHUT9_002909"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFR1349335.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            12843..13319
                     /locus_tag="ACHUT9_002910"
     CDS             12843..13319
                     /locus_tag="ACHUT9_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFR1349336.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            13633..13713
                     /locus_tag="ACHUT9_002911"
                     /pseudo
     CDS             13633..13713
                     /locus_tag="ACHUT9_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13723..13809)
                     /locus_tag="ACHUT9_002912"
                     /pseudo
     CDS             complement(13723..13809)
                     /locus_tag="ACHUT9_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13901..>14715
                     /locus_tag="ACHUT9_002913"
     CDS             13901..>14715
                     /locus_tag="ACHUT9_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1349337.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
BASE COUNT         3470 a         4000 c         3664 g         3581 t
ORIGIN      
        1 ttcaccatca ataaatttaa aaccacgcat acaaaatact tttgtattta tggatacgga
       61 aaacgattcc gctcgctcat aaggcatatt ttatacctgg tacttgttaa gtgtcgtttt
      121 tagtaagaaa aaaatcccga cacatggccg ggatagtgat ggtgaaaaag aattaacggc
      181 ggttgccgaa gatccgtaac aacatcagga acaggttgat gaagtccaga tacaaggtta
      241 acgcgccaag aatggaatat ttgcgcaggt tcgacgtgtc gcgggtatca atctgctcac
      301 ccatattttt caatttctga gtgtcatacg ccgtcaatcc gacaaagaca atcacgccga
      361 tgtaggtaac tgcccacatc aatgcttcgc ttttcagcca gaagttgacc agcgatgcca
      421 gcacaatgcc gattaacgcc ataaacagca tattgccaaa gccacttaaa tcgcgcttcg
      481 tggtgtaacc gtacaggctc attgcgccga acatcccggc agtaacgacg aaagtactgg
      541 cgatagaagc agcggtatag acaatgaata tactggaaag cgtaagaccc gtcagcgccg
      601 aataaagcat aaagagcatc gtcgttacac ctgcgctcag cttttgaatc atcgctgata
      661 acacaataac caatgctaat tgcgcgatga tcagaccgat taaaaagaca cggttagtga
      721 acaacagctc catcacggcc gcggaattag ccgcatacca ggcaacaaat gcggtcagca
      781 acaagccaac ggtcatccag ccatagactt gcgccatata agtttgcaag ccagcccggg
      841 gttgtacgat tgaatcagaa cgtgggaatc tgtccatgac gatctcctga agatataagg
      901 aatatcttaa ggatactgca aaatgatgag gctgtgcatc gacgcagcgt aaacgcatgt
      961 actgagcggt gaaattgccg gacgcagcgg tgccttatcc ggctaacaaa aaactaccag
     1021 cgttttgccg cctgctgatc gctctcccgg gcttcaaccc agcggtcgcc ttccggcgtg
     1081 gcttcgcgct tccagaacgg tgcgcgggtt ttgagataat ccataataaa ctgcccggct
     1141 tcaaacgcac tgctgcgatg cgcactggtg acaccgacaa aaacgatttc atcgcccggc
     1201 cataattccc cgatgcggtg aatcaccgtg acgcgcccca gcggccagcg gttacgcgct
     1261 tcatcaacga tttctgccag tgctttttca gtcatccccg gatagtgttc gagggttaat
     1321 gctttgacgc tgtcacccag gttatggttg cgcaccttac cggtaaacgt gaccactgca
     1381 ccgtcttcgt cacgctcagc cagccacggg tactcttctc ctacgctgaa cggctgcggg
     1441 ccgacaacaa ttttggtttc tgccatctta acctccggtt accggcggga agaaagctac
     1501 ttcgtcgccg tcagtcagcg gatggtcaaa actcaccagc gtctggttga cggcagccag
     1561 caatttgcca tcttccagcg ccagcgccca gcgatcgctc tgcgcagcca ggtgctggcg
     1621 taacgcttca acggttgggt aatccgcagc cacttcggtt gcatctgttc ccaccaactc
     1681 gcgcacctgg gcgaaaaaaa gaactttaat catccgcttc caccttaaag tcacccgact
     1741 tgccgccgct cttcgccagc aaacgtaccg gaccaatcac catatctttt tgcaccgctt
     1801 tgcacatgtc ataaatggtc agcgccgcca cggaggccgc ggttaacgct tccatttcga
     1861 caccggtttt cccggtcagg cggcataagg tttctatacg cacccgattg tgctccggct
     1921 cggcctgtaa attgacttca actttgctga gcatcagcgg gtgacacagc gggatcagat
     1981 cccaggtgcg ttttgccgcc tgaataccga caatacgcgc agtggcaaat acgtcgcctt
     2041 tgtggtggcg accatcaata atcatcgcca gcgtctcgct gcgcatggtg acaaaggctt
     2101 ccgcccgcgc ttcacgcacg gtttccgctt tggcggagac atccaccatg tgcgcttcgc
     2161 cagcggcgtt gatatgggtc agttgcgaca tacttatttc ttcaaatgtg gatggaaatt
     2221 acacggacgc gtacgggcat ccagctgcgg cgcgatgata ttttcccatg cggtacggca
     2281 cgctttggtc gaacccggca tggcgaaaat cagcgttttg ttggcgacgc ccgctaccgc
     2341 acgagattgc aacgtggaag tgccaatctc ttcaaacgac aacatccgga acacttcacc
     2401 aaaaccttca acttcacggt cgaacaacgg cagcaatgct tcgggagcct gatcaccttc
     2461 cgtcaggcca gtcccccccg taatcaacac cacttgtaca tcgtcgctgg cgatccacgc
     2521 agatacctga gcgcgaatag cgtagcggtt ttctttcaca atggctttat cgacaacgtg
     2581 atggcccgct tcttgcgccg aatcgcgcaa atagtgaccg gaggtatcgt cttcttcacc
     2641 gcgacgatta gaaaccgtaa gaatagcaat acgggtcggg ataaattcag tgcttacctg
     2701 actcatctga tctctccttt tgacgtttta gccgccaatg tacgataagt tttgcgtaat
     2761 accggtgttg ttttgatgca ggaaatgggt ctgtttcttc tcccgcagcg ccgctgaaat
     2821 acgcgcttcc agcgcctgtt gctgggtatc gtcttccagc agatcgcgca ggttaacgcc
     2881 gccttcacca aacaggcaga gatggagttt accaatggag gaaacgcgca ggcggttgca
     2941 agtggcgcag aagtcttttt catacggcat gataaggcca atctctccgg cgtaatctgg
     3001 atggcaaaag acttgcgcgg gaccgtcgct gcgttgacgt aattggtgga tccagccgcg
     3061 acgcagtagc tcgtcacgca gaacctgacc agagatgtga tgcttacgga agagctcact
     3121 gccctcgccc gtttccatca gttcgatgaa acgcagctgg atagggcgat gctggatcca
     3181 gttcagaaag gtgtcgagct ggtgatgatt aacatcacgc atcagcacgg tattgacttt
     3241 gaccttctca aaaccgacct caaatgcagc atcgatccct gccatgacct ggttgaattt
     3301 atcctgcccg gtaatagcgt gaaactggcg ggcgtccaga ctgtcgacac tgacgttaat
     3361 gccagtaagt cccgcatcgc gccagttcgc cacatcgcgt tccagacggt aaccattggt
     3421 ggtgaccgca atctggcgga tagcgtcgtt ttcccgcaca gcggcgatga tatcggtaaa
     3481 gtcgcggcgt aaagacggct ctcctcctgt caggcgcact ttttcggtgc ccagactggc
     3541 gaaggcgcgc gtaacccggc gaatttcatc gacggtaaga aagcctttat tggtgacgcc
     3601 gctcggtttg tagccatccg gcaggcagta ggtgcaacga aagttacaca catcggtaat
     3661 cgacaggcgc aagtagtaaa acttacgcgc aaatgcatca gtcagttgtg aagccatgta
     3721 cacctttcca gatacgggag gcgaagtcat ttcttccttc gccctggtgg caatctttac
     3781 gagcattgtc acggccaaag caccgtatca gttgacccag gtgcagaggc tagagtgttt
     3841 agtggttaag ccgatactag cgtgtaaatg agatttttac catccacact ttcgctatgt
     3901 aatcatgtat atagcgtcat gatcgtgcta tttcgctaca gaataaggga aaatggcagt
     3961 tttactttga tatacatcat cagggaggca tagcgtgcat cgtcctttag aggtagaaga
     4021 aaatagtttg tatcctcaat attggcaggt taattgctgt ttccccgcaa tttgcgctac
     4081 tgtagcgcgg ataatctgac tccaggaaac tatatgcgca atcgtacgct ggctgatctt
     4141 gatcgtgtcg ttgctctcgg cggagggcat ggactgggac gcgttctctc atcactttcg
     4201 tctttgggtt ctcgtttaac gggtatcgtc accaccacgg ataatggtgg ctcgacgggg
     4261 cgtattcgcc gttcagaagg cggcattgcc tggggcgata tgcgcaactg cctcaaccaa
     4321 ctgataacgg aaccgagcgt cgcctccgcg atgtttgaat accgttttgg cggcaatggc
     4381 gaactttccg gtcataatct cggaaacttg atgttaaagg cgctggatca ccttagcgtg
     4441 cggcctctgg aagccatcaa tttaattcgt aatctgctga aagtggatac gcatttgatt
     4501 ccaatgtcag agcatcccgt cgatctgatg gcgattgacg atcaggggca tgaagtttac
     4561 ggcgaggtca atatcgacca gttaactacg ccgattcaag agttattatt aacgccgaat
     4621 gtacccgcaa cgcgtgaggc ggttcacgct atcaatgaag cggatctcat cattattggg
     4681 cctggcagtt tttataccag cctgatgcca attctgctgc tgaaggaaat cgcccaggca
     4741 ttacgccgca cgccagcgcc gatggtctat atcggcaatc tggggcgtga gttgagttta
     4801 cctgcggcta atttgaagct ggaaagcaag ctggcaatta tggagcagta tgttggtaaa
     4861 aaggtcattg atgcggtcat cgtcgggcca aaagtggatg tctcggcggt gaaagagcgg
     4921 attgtgattc aggaggtact ggaggccagc gatattccgt atcgtcatga ccgccagttg
     4981 ttacataacg cgctggaaaa ggcgttacag gctttaggtt aacggaaatc cgaagaaaaa
     5041 ttccggcttc ctattgaaga caaagtgcgc gttgtttatg ccggatgcgg cgtgaacgcc
     5101 ttatccggcc tacaaaccgc gctaattcaa tgtattgcag agatcatgta ggcctgataa
     5161 gcgtagcaca tcaggcaatt ttgcttttgt catctgtctt cttcgttatc ctgtcacgaa
     5221 gccgcgataa acagctcacg cagctgatgc aactggtcac gaatttgtgc cgcttcttcg
     5281 aactccagat tctgcgcgtg ttgcatcatt aacccttcca gctcatggat tttctgttgc
     5341 aacgctttag gcgacatatc catcggcaca ttatccggct caacaatcgg gcgcgatttt
     5401 cctctgccct tcgctttggt tttggcaatg ttctgcccca gcgccaggat atcgaccact
     5461 ttcttgttca agccttgcgg cgtaattccg tgttcctcgt tgtacttctg ctgtttctca
     5521 cggcgacgtt cggtttcgcc aatcgctttc gccattgatg gggtgatctt atcgccgtag
     5581 agaatcgctt taccgttaac gttacgtgcc gcacgaccaa tagtctggat caacgaacgt
     5641 tcggaacgca ggaagccttc tttgtcagcg tcgaggatcg ccaccagcga cacttccggc
     5701 atatccagac cttcgcgcag taagttgatc cctaccagca cgtcgaactc acccagacgc
     5761 aagtcgcgga taatctccat acgttcgacg gtgtcgatat ctgaatgaag ataacgcacg
     5821 cgctcgccat gttcttcgag atattcggta agatcttccg ccatccgctt ggtcagtgtg
     5881 gtgaccagta cgcgttcgtt aattgccgct cgctgacgaa tctccgaaag aagatcatca
     5941 acctgtgtcg ccaccggccg cacttcgata atcgggtcaa gcaatccggt tggacgcacc
     6001 acctgatcca ccacatcgcc gccggatttt tccagctcgt aattacccgg cgtcgccgaa
     6061 acatagatgg tttgcggcgc taatgcttcg aactcttcaa acttaagcgg acggttatcc
     6121 agcgctgatg gcaggcggaa gccgtactcc accagtgtct ctttacgcgc ccggtcaccg
     6181 cgatacatgc cgccaatttg tggaatggtg acgtgagatt catcgaccac cagcagccca
     6241 tcggcaggca ggtaatcaaa cagcgtcggc ggtggctcac ccggtccacg accggagagg
     6301 aagcgcgagt agttttcaat ccccgaacag tagcccagct cgttcatcat ctccagatca
     6361 aactgggtac gctgggtcag ccgctgctct tccagcagtt tgttgttttc caacagcact
     6421 ttgcgtctgg cggccagttc ttctttgatc tcctccatcg cctgtacgat gcgctcgcgc
     6481 ggtgtgacgt agtgcgtttt cgggtagatg gtaaaacgtg gaatagtgga aacaatctgc
     6541 ccggtcagcg ggtcaaataa cgacaatcgt tccacttcct cgtcaaacag ttccacgcga
     6601 agtgcaatgt catccgattc tgccgggaag atatctatca cctcgccacg aacgcggaaa
     6661 gtaccacgct ggaatgcttg atcattacga gcgtattgca gctccgccag tcggcgcaga
     6721 atcgcgcgct gatcgataat cataccgacc gtgagatgga gcatcatctt gagatataaa
     6781 tcaggatcgc ccagaccata aatcgcggaa acagaggcca ctacaaccac atcacgccgc
     6841 tccagcatcg ctttggtggc ggataaacgc atctgctcaa tatgttcgtt aaccgaggca
     6901 tctttctcaa tgaaagtgtc ggaactcggt acataggctt ccggctgata gtagtcgtag
     6961 taggagacga aatactccac cgcgttttcc gggaagaact ctttcatttc gccatacagc
     7021 tgggccgcca gcgttttgtt gggcgcaagt accatggttg ggcgctgaag gtcagcaatg
     7081 acattggcaa tggtgaaggt tttccctgag ccagtcacgc caagtaacgt ctggtgcgcc
     7141 aggccatctt ccagcccctc ttcgagacgt cgaatcgcct ctggctgatc gccagaaggt
     7201 ttaaaagcgg aattcagttt gaacggttta ctcatgagtc gctacctgaa ggagttgggc
     7261 gggcaggtat gtaattttac tcgtcgtact taattatgcc aacaaattat actggataaa
     7321 aaaacagttc atcaccataa tatttctgat acagcgtaaa ctccgctgtc aatcatgagc
     7381 aaaatttact ctgtggcgag ataaaactcc ggccttaccg ggttatcccc aaagcaacgg
     7441 cttttttaac atttgtcaag atgagtgatg acagttttat ggcaagagat gcctgttcag
     7501 tgactgccat tgcatttatc taaccagtta aaaaataaaa gatatttctt tgagccgtct
     7561 ttaacgccaa ttcgcgtgca agccgcgtat tctctcgctt gcctcgtgtt ttctaactct
     7621 aatacacatg gttatccaca ggaatagtgg ataactgtct ccagccccta tcccccgccg
     7681 cctgggcaat tcccacattc gccgcatgag gcggaagtaa aatttttttc gtgattattt
     7741 tagaatttaa ttggttaaat tgcagtcaat cgaagacgcg atctcgctcg caatttaacc
     7801 aaatacagaa tggctacaac aaggcaaggt ttatgtactt tccggttgcc gcattttctg
     7861 caagccaggg gatctctccc agcagcggcg cgggaatcat gcgggtgagc gtggtcatat
     7921 attcagcgtg acgttttccc ggaggcgtaa catcgttcgc cacccaaccc gccagagtca
     7981 gtccggcgtg ttgtattgcc tgcgcagtca acatcgcgtg attaatacag ccgagtttca
     8041 caccaactac cagtatcacc ggcagttgtt cctgtgttac ccaatctgca aaagtgaaag
     8101 tgtcagaaag cggcgtaaac cagccgccag caccttccac taacacccag tcagcctgtt
     8161 gttcaagcgc gcgtaatccg gcgctcatta ccaatgattc tatcggcctg ccctcttgcg
     8221 cgctgatgat gtgcggcgaa gtgggttctg cgaaggtgta aggatttact gttgcgtaat
     8281 ccagctgcag gctgctgttg tgctgtaacg ccagcgcgtc actattacgt aagccttccg
     8341 gggtcttttc gctgccagag gcgaccggtt tataacctgc cgtccgataa cctactgcct
     8401 tcgcggcttg taaaagtgca caactggcga cggttttccc cacttcggta tccgttccgg
     8461 tgacaaaata acgtttactc acgagcaatc actcccaaaa aaagatgata cgtcagagga
     8521 tatcgcccct gttgttgcgg ccaggccagt tgcaatcgct gcaattgcga acgcgttaat
     8581 attcgcgggt cgcgcccttc atgaagatgc gtagcaccga tgcctttcag cgaacgcatg
     8641 gcactgagcg catcatcaaa ccatagtgtg atgggctgaa tatgatgttg ataatgcacg
     8701 ccgttcagcg actgttcgat ttcatctggc ggtaaaaagc gattagcatg cggacgctcg
     8761 tccaccgcct gccacgcctg atgcagttcg ggtaacgatc cctgcaccag cgtggtaaac
     8821 gccaccacgc ctccggggcg caccacccga tacagctcgc ggagtgccgt ggataaatta
     8881 ccgcaccact gcaccgcgag attgctccat gcaagatcga acgtcgcagt cgctaacggc
     8941 agggattcga tatctcccgc cagataatgg tctgcagcat ctttctggcg ggcttgcaca
     9001 agcattggcg gtgagagatc taacgccgtc acctgtgcgt gacgctctcg ccagtggcgg
     9061 ctcatccagc ctggcccaca ccccgcgtcc agtacgtggg tgtatttacg ctgtgggagc
     9121 attgccagta agacgtcagc actccggcgc tgtaactctg cgtgttgctc atagtgcgcg
     9181 gctgcccgac caaatgccgc tgcaatggct tgtttattaa ccgttgccat gcagcacctc
     9241 cagcagacgg tcgatatcct gcatttcatg cgctgcggtt agcgttaagc gcagtcgcgc
     9301 agtaccagcg ggtacggttg gcgggcgaat cgccgtgacc cagcagcctt gctggcgcag
     9361 tttttctgcc agttgtaacg cacggctgtt atcaccgacg atcaatggct ggatggcgct
     9421 gcatgaatca gcaagcgtaa acggcaaatc ctgtactccg gcacgaaaac gcgcaatgag
     9481 tgacaccagt ttttcgcgcc gtgcatcccc ctcatcacgg cgaatgaccg ccagcgacgc
     9541 acgtaatgcc tgcgcctgag cgggcggcat actggtgctg tagataaggt ggcgggcgaa
     9601 ttgcagcaga taatccgcca ccgtactgga gcaaagcacc gcagccccac tgacgccaaa
     9661 tcctttgcca aaagtcacca ccagcaattc tggttttacc ttttgcagcc agcagctgcc
     9721 gcgcccctgc tccccgataa cgcccgtgcc gtgggcatca tcgaccatca accagccatt
     9781 gtgctgttgc gttacctgct ggatttccgc cagtggcgcg ctatcgccgt ccatgctgaa
     9841 cacgccttct gtcaccacca gttgctgccc cggacagggg gaagcaagca gtcgcgccag
     9901 atgagcgaca tcgttatgag taaaacggcg aagctgcgac gggcttaaac tggcagcctc
     9961 cagcaaggag gcatggctaa gccggtcggc aacaatacgg tcctctttcg ccatcatcgc
    10021 ggcaatgact gcctgattcg cggcaaaacc agagataaac agcagcgccc gcgaatagcc
    10081 aagccactcg gccagttctt cttccagcgc ctgatgcacc acgctataac cgctgacatg
    10141 cccggaaccg ccgctaccta caccaaactt ttctgcgccc tgctgccagg cgcggataat
    10201 ttgcggatga tggcttaaac ctaaataatc gttactggaa aagttcagat actggcgatc
    10261 atccgccacc agccagcgtc cggctccttg cgccaccgga taacggcgac gcagggcatc
    10321 ggcagtacgc cgtgcatcga gcgccgcgtt gattttctcc tgccagctca taatgctgcc
    10381 gcgttgtaat attcgtcggt gtccggggtc atcagcgcct gttcaagacg ttgctgttgt
    10441 tcgttatccc ctgccagcac ggcagtttgc tgcggattta gccccagttt gcggaacagt
    10501 tgcaggtctt tatcttcttc cggattcggc gtggtcagca gtttgcaacc gtagaaaatc
    10561 gagtttgcgc cagccataaa gcacatcgcc tgagtctgtt cattcatctg ctcgcgtccg
    10621 gcagaaaggc gcacgtaaga ggtcggcatc atgatccgcg ctaccgcaat agtgcgaata
    10681 aaatcaaagg catcgacatc atcgttatcc gccagtggcg tgcctttcac cttcaccagc
    10741 atgttgattg gtacgctttc cggcggcgtc ggcaggtttg ccagttgcag caataatccg
    10801 gcgcgatctt ttaccgtttc gcctaagcca acaatgccgc cagaacagac tttgatcccg
    10861 gcatcgcgca ctttttccag cgtatcgagg cgttcctgat aagtgcgggt ggtgatgata
    10921 ttgccgtaaa actccggcga ggtgtccagg ttgtggttgt aataatccag cccggcgttc
    10981 gcgaggcgct gcgcctgaga ttcactcaac gtgcccagcg tcatacacgc ctccagcccc
    11041 atcgctttta ccccctgcac catttgttcc aggtacggca tatcgcgttc gtggggattc
    11101 ttccacgccg cgcccataca gaagcgcgtc gatcctgccg ctttcgcttt gcgcgccgac
    11161 tccagcacct gttcaacttc catcaaccgc tcggcttcca gcccggtttt gtagcgtgag
    11221 ctttgcgggc agtatttgca atcttccgga caagctccgg tcttaatcga cagcaacgtg
    11281 ctgacctgga cctgacgagg atcgaaatgc tggcgatgga cctgctgcgc ttcaaacagc
    11341 agatccagca acggtttttc aaataattct gtgacttgcg acaaggtcca gcgtgggcgg
    11401 tgagccatgg ggcttctcca aaacgtgttt tttgttgtta attcggtgta gacttgtaaa
    11461 cctaaatctt ttcaatttgg tttacaagtc gattatgaca acggacgatc ttgcctttga
    11521 ccaacgccat atctggcacc catacacatc catgacctcc ccgctgccgg tttatccggt
    11581 ggtgagcgcc gaaggttgcg agctgatttt gtctgacggc agacgcctgg ttgacggtat
    11641 gtcgtcctgg tgggcggcga tccacggcta caatcacccg cagcttaatg cggcgatgaa
    11701 gtcgcaaatt gatgccatgt cgcatgtgat gtttggcggt atcacccatg cgccagccat
    11761 tgagctgtgc cgcaaactgg tggcgatgac gccgcaaccg ctggagtgcg tttttctcgc
    11821 ggactccggt tccgtagcgg tggaagtggc gatgaaaatg gcgttgcagt actggcaagc
    11881 caaaggcgaa gcgcgccagc gttttctgac cttccgcaat ggttatcatg gcgatacctt
    11941 tggcgcgatg tcggtgtgcg atccggacaa ctcaatgcac agcctgtgga aaggctatct
    12001 gccagaaaac ttgtttgctc ccgccccaca aagccgtatg gatggcgaat gggatgagcg
    12061 cgatatggtg ggctttgccc gcctgatggc ggcgcatcgt catgaaatcg cggcggtgat
    12121 cattgagccg attgtccagg gcgcaggcgg gatgcgcatg taccatccgg aatggttaaa
    12181 acgaatccgc aaaatatgcg atcgcgaagg tatcttgctg attgccgacg agatcgccac
    12241 tggatttggt cgtaccggga aactgtttgc ctgtgaacat gcagaaatcg cgccggacat
    12301 tttgtgcctc ggtaaagcct taaccggcgg cacaatgacc ctttccgcca cactcaccac
    12361 gcgcgaggtt gcagaaacca tcagtaacgg tgaagccggt tgctttatgc atgggccaac
    12421 ttttatgggc aatccgctgg cctgcgcggc agcaaacgcc agcctggcga ttctcgaatc
    12481 tggcgactgg cagcaacagg tggcggatat tgaagtacag ctgcgcgagc aacttgcccc
    12541 cgcccgtgat gccgaaatgg ttgccgatgt gcgcgtactg ggggccattg gcgtggtcga
    12601 aaccactcat ccggtgaata tggcggcgct gcaaaaattc tttgtcgaac agggtgtctg
    12661 gatccggcct tttggcaaac tgatttacct gatgccgccc tatattattc tcccgcaaca
    12721 gttgcagcgt ctgaccgcag cggttaaccg cgcggtacag gatgaaacat ttttttgcca
    12781 ataacgagaa gtccgcgtga gggtttctgg ctacactttc tgcaaacaag aaaggagggt
    12841 tcatgaaact catcagtaac gatctgcgcg atggcgataa attgccgcat cgtcatgtct
    12901 ttaacggcat gggttacgat ggcgataata tttcaccgca tctggcgtgg gatgatgttc
    12961 ctgcgggaac gaaaagtttt gttgtcacct gctacgaccc ggatgcgcca accggctccg
    13021 gctggtggca ctgggtagtt gttaacttac ccgctgatac ccgcgtatta ccgcaagggt
    13081 ttggctctgg tctggtagca atgccagacg gcgttttgca gacgcgtacc gactttggta
    13141 aaaccgggta cgatggcgca gcaccgccga aaggcgaaac tcatcgctac atttttaccg
    13201 ttcacgcgct ggatatagaa cgtattgatg tcgatgaagg tgccagcggc gcgatggtcg
    13261 ggtttaacgt tcatttccac tctctggcaa gcgcctcgat tactgcgatg tttagttaat
    13321 cactctgcca gatggcgcaa tgccatctgg tatcacttaa aggtattaaa aacaactttt
    13381 tgtcttttta ccttcccgtt tcgctcaagt tagtataaaa agctgaacga gaaacagtaa
    13441 aaatcattaa tatcaatgca ttataataca tttagcttaa aaaatagact gcataatact
    13501 gcaaaacaca acatatccag tcactatgaa tcaaccactt agatggtatt agtgacctga
    13561 tacagagcat tagcgcaagg tgattttttg tcctcttgcg ctaatttttt gtcaacgcac
    13621 tgggttagct gaatttacaa ccatgctcag tatctcgata agcgcagaga aatgatgcag
    13681 tggtgggcag actactggct tgatgaaaag gtagggcgac caaccgcgtt ttttcagttc
    13741 agtcagcaca ttgagccaga acttcacccc ttcattttcg gccagccaca tccccaacag
    13801 ttcttcctgt tcttaactat ttacaggcgc gtttttttaa agaatcctca ctccatttgc
    13861 acgaacatat taatatataa acaatgagga tcaataataa atgaaacctg cccacaatcc
    13921 ttcttttttc cgctcctttt gtggtttagg atgtatatcc cgtttatccg tagaagagca
    13981 aaatatcacg gattatcacc gcatctggga taactgggcc aaggaaggtg ctgcaacaga
    14041 agaccgaaca caggcagttc gattactgaa aatatgtctg gcttttcaag agccagccct
    14101 caatttaagt ttactcagat tacgctctct cccatacctg cccccgcaca tacaagaact
    14161 taacatctct agcaatgagc tacgctctct gccagaactc cctccgtcct taactgtact
    14221 taaagccagc gataacagac tgagcaggct cccggctctt ccgcctcacc tggtcgctct
    14281 tgatgtttca cttaacagag ttttaacatg tttgccttct cttccatctt ccttgcagtc
    14341 actctcagcc cttctcaata gcctggagac gctacctgat cttcccccgg ctctacaaaa
    14401 actttctgtt ggcaacaacc agcttactgc cttaccagaa ttaccatgtg aactacagga
    14461 actaagtgct tttgataaca gattacaaga gctaccgccc cttcctcaaa atctgaggct
    14521 tttaaacgtt ggggaaaacc aactacacag actgcccgaa cttccacaac gtctgcaatc
    14581 actatatatc cctaacaatc agctgaacac attgccagac agtatcatga atctgcacat
    14641 ttatgcagat gttaatattt ataacaatcc attgtcgact cgcactctgc aagccctgca
    14701 aagattaacc tcttc
//
LOCUS       DAZPEP010000101        14621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZPEP010000101 DAZPEP010000000
VERSION     DAZPEP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.101"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            73..1608
                     /gene="lsrA"
                     /locus_tag="ACHUT9_002914"
                     /pseudo
     CDS             73..1608
                     /gene="lsrA"
                     /locus_tag="ACHUT9_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1602..1778
                     /locus_tag="ACHUT9_002915"
                     /pseudo
     CDS             1602..1778
                     /locus_tag="ACHUT9_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(1786..1875)
                     /locus_tag="ACHUT9_002916"
                     /pseudo
     CDS             complement(1786..1875)
                     /locus_tag="ACHUT9_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2082..3530)
                     /gene="uxaB"
                     /locus_tag="ACHUT9_002917"
     CDS             complement(2082..3530)
                     /gene="uxaB"
                     /locus_tag="ACHUT9_002917"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFR1349338.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3757..4707)
                     /locus_tag="ACHUT9_002918"
     CDS             complement(3757..4707)
                     /locus_tag="ACHUT9_002918"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFR1349339.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4846..5205)
                     /locus_tag="ACHUT9_002919"
     CDS             complement(4846..5205)
                     /locus_tag="ACHUT9_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFR1349340.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(5205..6131)
                     /gene="glsB"
                     /locus_tag="ACHUT9_002920"
     CDS             complement(5205..6131)
                     /gene="glsB"
                     /locus_tag="ACHUT9_002920"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFR1349341.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(6195..7583)
                     /gene="sad"
                     /locus_tag="ACHUT9_002921"
     CDS             complement(6195..7583)
                     /gene="sad"
                     /locus_tag="ACHUT9_002921"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFR1349342.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            7684..8565
                     /locus_tag="ACHUT9_002922"
     CDS             7684..8565
                     /locus_tag="ACHUT9_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1349343.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            8642..9757
                     /gene="yneK"
                     /locus_tag="ACHUT9_002923"
     CDS             8642..9757
                     /gene="yneK"
                     /locus_tag="ACHUT9_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFR1349344.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            9907..11097
                     /gene="ydeA"
                     /locus_tag="ACHUT9_002924"
     CDS             9907..11097
                     /gene="ydeA"
                     /locus_tag="ACHUT9_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFR1349345.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(11122..11787)
                     /gene="marC"
                     /locus_tag="ACHUT9_002925"
     CDS             complement(11122..11787)
                     /gene="marC"
                     /locus_tag="ACHUT9_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFR1349346.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            11999..12433
                     /gene="marR"
                     /locus_tag="ACHUT9_002926"
     CDS             11999..12433
                     /gene="marR"
                     /locus_tag="ACHUT9_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFR1349347.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            12453..12836
                     /gene="marA"
                     /locus_tag="ACHUT9_002927"
     CDS             12453..12836
                     /gene="marA"
                     /locus_tag="ACHUT9_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFR1349348.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            12868..13086
                     /gene="marB"
                     /locus_tag="ACHUT9_002928"
     CDS             12868..13086
                     /gene="marB"
                     /locus_tag="ACHUT9_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFR1349349.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(13143..14582)
                     /locus_tag="ACHUT9_002929"
     CDS             complement(13143..14582)
                     /locus_tag="ACHUT9_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1349350.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         3787 a         3745 c         3391 g         3698 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgaaaggca tcgattttac gttgcatcaa
      181 ggggaggtcc acgccctgct cggcggcaat ggtgccggta aatcgacgtt aatgaagatt
      241 attgccggta ttacccctgc tgatagcggt acgctggaga ttgggggcaa caactacgtc
      301 agattaacgc cagttcatgc tcatcagctg ggtatttatc tcgttcccca ggaaccgctg
      361 cttttcccaa gcctgtcgat aaaagaaaac atcctgtttg ggctggcaaa aaaacagctc
      421 tccatgcaga aaatgaagaa cttgctggcg gcgctgggct gctagtttga tctgcatagt
      481 ctggcaggat cgctggatgt cgccgatcgc caaatggtgg aaatcctccg cggcctgatg
      541 cgcgactcgc ggattctgat cctcgatgaa cctaccgcct cgcttacccc tgcggaaacc
      601 gaacgcttgt ttcgtcgctt gcaagagctg cttgctactg gcgtgggtat tgtttttatc
      661 tcgcataagc tgccggaaat tcgccagatt gccgatcgaa ttagcgtgat gcgcgacgga
      721 accatcgcct taagcggcaa aaccagcgaa ctgtctaccg atgacattat tcaggccatc
      781 acgccagtgg tacgggaaaa atcgctctct gccagccaaa aattatggct ggagttacct
      841 ggcaaccgcc cacaacatgc cgccggaacg tcggtgctga cactagaaaa tctgaccggc
      901 gaaggtttca ggaatgtcag cctgacgctc aatgccggag aaattctggg tctggccggg
      961 ctggtggggg ccggacgcac agaactggcc gagacgctct atggtctgcg tactttgcgt
     1021 ggcggacgca ttatgctgaa tggtaaagag atcaataaat tatccactgg agaacgttta
     1081 ctgcgcggtc tggtttatct gccggaagat cgccagtcat ccggactgaa tctcgatgct
     1141 tcgctggcct agaacgtctg cgcccttact cataaccttc gtggattctg ggcgaaaacc
     1201 gcgaaagata atgccaccct ggaacgttat cgtcgggcgc tgaatattaa atttaaccaa
     1261 ccggaacaag ctgcacggac attatccggt ggcaaccagc aaaaaatcct gattgccaaa
     1321 tgcctggaag cttcgccgca agcattgatt gtcgatgagc cgacgcgcgg cgtggatgtc
     1381 tcggcccgta atgatatcta ccagctgttg cgcagcatcg ccgcacagaa tgtggcagtg
     1441 ctgcttatct cctctgatct ggaagagatc gaactgatgg cagatcgtgt gtatgtgatg
     1501 catcagggcg aaattaccca ctctgcactg accgggcgcg atattaatgt cgagactatt
     1561 atgcgcgttg ccttcggcga tagtcagcgt caggaggcgt catgctgaag tttattcaga
     1621 acaaccgtga aatcacggca ctgctggcgg tggtgctgct gtttgtatta cccggttttc
     1681 tcgaccgcca gtatttaagt gtgcaaacgc tgaccatggt ttatagcagc gcgcaaatcc
     1741 tgatcctgct ggcaatgggc gcgacgctgg taatgcttat gcgcaaagta gtaagcgcga
     1801 tgatatcttg gataatactg agccgtgcca gacaaaaata cggcgcagcc agtgttgttg
     1861 tggacgaaca atcatggtta tcttcaggcg tgaaaaagtc gccctattaa acgtgattac
     1921 gatcacattc tcaagacgct acttacaaat tacctacaag cttacaacat taacaggcgc
     1981 tgcatgtggc atctgttaat gataaaggca cataggatgt tgtaactaat atagtgaaat
     2041 aagaaacccg gtcaaaaccg ggttcagaag taacggtgtt attagcacaa cggacgtaca
     2101 gcttcgcgca tccctttttc aagaatcgca tccaggtcat tagcaacctg ctcgaccaga
     2161 ccaggcactt gcgtcaggtc ctgctcccag tggtcttttt ctgccaacac aatcgctacc
     2221 agttcctggg taccgataac atggtcacga tgctggctcc agagttgctg ataacgttcc
     2281 agccagtgtg catcgtcctg taccggatat gtttctccgt tacgctcacc acgatagaac
     2341 gcaatcaatg ccgctaatgc gaaagtaagg cgcgccggaa gtgtgccgtt tgccttctgc
     2401 cctgccagca gctgcggcag gatgcgggtg cggaacttgg tcataccgtt gagcgcgata
     2461 gacagcagct gatgcttata cgggttacgg aaacgcccgg tgactgcact ggcgaaagat
     2521 tccagttcat cacgaggcaa atccagtacc ggaataattt cttcgtagat ggctttttca
     2581 acgaatgcgc aaatttcagc atcgttcatc gcttcaccta cggtatccag ccctgcctgg
     2641 aacgccaccg gcaccagcgc ggtgtgcgca ccgttgagga ttgccacttt gcgttcttta
     2701 tacggtttaa tatcgtcaac aatcagcacg ttgagcggat atttgtccag gcgcagctca
     2761 gtcgctaagg atttcggccc ctgaatcaca aacaggtaaa agtgttcagc ggtgtcgagg
     2821 aaaccatcgt gataacccag ttcttcttcc agtttagcca cttcatcgcg cggataaccg
     2881 gtaacgatac ggtccaccag cgtagaacag aagctgttag cctgatccag ccattgaata
     2941 aatgcttctg gcagtgccca ctcttgcgca tagcgcagca ccagttcacg caacgcgtcg
     3001 ccattgtagt cgatcaactc acacgggatg atgatccaac ctttatccag cgcaccgttg
     3061 aaatggctaa agcgttcgaa cagcagacgg gtcagttttg ccggatagct tatcgctggc
     3121 gcgtcatcga atttatcgcc cgcatggtag ctgatccccg cttcggtggt gttagagaaa
     3181 acaaagcgca tttccgggtt gtgcgccagt ttcaggaatt catcgtattc actgtagacg
     3241 ctgatttcac gattaaccga gcgaatcaga cgcgcgtcgc tgaccgcttc ccccttctca
     3301 ttcaggccac ggataatggt ggtatacaga ccatcctgcg tgctcagtga cggcgggaat
     3361 gaagtttcaa tcggacgaac aacgaccacg ccagaattca gatcggtgtg ctcattcagg
     3421 agatcgattt gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa ctgaatgata
     3481 cgttctggat actgtgcacc gggaaaatcg cgacgattta gtgttttcac aatgggttcc
     3541 cttctgatta gtcatacaac ctgtttgaat tggtacgaca ggttagcaaa ctttaatacg
     3601 ccgaacccct gttttgatca actcctgatg attaatgagc agttttatga gaaaagtgtg
     3661 gcgcggatca tggtttaatc gaggaaatac gccttttcct ggatcataaa gtggtagaac
     3721 acattgcatt caaatcgcgc ggaatgaata aagatgtcag acaacttcct caccgtaacg
     3781 catagtgctg gtacggttgc gcccatcttt cttcgagcga tacagacacg tatcggcttc
     3841 gaccatcaat ttattaaaat catcggtcag cgttcggtac gatgcgcgac cactaccgac
     3901 gccaatactt accgtgagat aaagcgtttt ttgttgccag gtgaatggtt gcagttcaac
     3961 gcctttacgg attttttccg ccattagcag accatcgaca ggattcaccg acggcactgc
     4021 aacagcaaat tcttcgccgc ccatgcgcgc caccagcccc ttatcaccga caatcttctg
     4081 aatatgccgg gcaaacacgc ttaacacttt atcgccacat tcatgtccat agttatcgtt
     4141 aatgcttttg aaatagtcga tatcaagcag catgaccgtc agaggcacga tcaacacgcc
     4201 cgttgagaat tgctcactga accagtccgc aagcaaaggc cagaaatcca gactatcaat
     4261 actgaccgaa ccaatcgccc cgacaatagc gcataataat gcggcaatca gacagtaatt
     4321 aaatagccgt aaggcactta ctggctcata cttatttttc cccagacgct tatcccgcac
     4381 gaccagtaag gcgacggtaa caataaacat catattggag aaattgataa ccagtgaaac
     4441 cagtccccat tcggtggtta tggcatcata aaccagcatc gccacataac tgatcgcata
     4501 gtaatgcagt cgattaagcc agacatagcg ggcaaacccc cccgccatta cgccattcag
     4561 cggccagaaa agagaaagtt cgtgaaccag ccgtaactct gcaccaataa aatagaacag
     4621 cgtggttagc gcaaaaatag cgatgctatt acgtagcaga tgaccttcct ggaacaaacg
     4681 gaaagttgaa atggggtgta catgcattaa ttatctgtcc atacaattgc gggtggcaat
     4741 tatctcaacg taataagtaa attctcataa aggatagcat gttcgctaac agcggcggca
     4801 aataaaggta ccctgggaat gctattttat cgttttccgc aattctcatg gctgattcat
     4861 ctgtacaacc agccagtgat agattaccgc cacgatataa cgttgttgtt cttcgcttaa
     4921 cgaaacaccc tgcggaatgt tatgccactt agccagacaa ccgcgacagc aggtcgcggt
     4981 ggcgtgctga gcgataaaca ccggatgccc gcgcatgggg gtttgcttac cgtcattagc
     5041 cggtaatgcc ggagcaagac gtttagcgac aaaatccgcc gcatgttgtt cgatgactgg
     5101 cgcgcctttc tccaggcaat actgacgctc tttcatgccc agacgaaagc gagagcgaaa
     5161 ttttgaacgg gataaacgcg cgaagagtgg atcgagagac tgcattaata aaccgaacgc
     5221 cctaactgtt tcgtcaattg ttcaaggacg gcgatacccg caagcgagtt acctgcatca
     5281 tccagttccg gactccagac tgcgatggcc atttcatgcg gaacaatcgc cacaataccg
     5341 ccaccaacgc cagatttcgc cggtagtccc acccgccagg caaactcccc cgcgttctgg
     5401 tacataccac tggtcgccat cagcgcgtta atttgccgcg cctgcattgg cgtcactaca
     5461 ggttcatcaa tatgaatagc tttcccctga ttagccagaa agacaaacgt ccgagccagc
     5521 tctacacagc tcattttcag agcgcagtaa tgaaagtagt tttgcagaac ggttgtcacg
     5581 tcatgatgga aattgccaaa cgacttcatc agccaggcga tagccgcatt tcgcgcggaa
     5641 tgttcaaatt cggaacgcgc taccaccgta tcgtatgaaa tatcagacac accgcttaag
     5701 ccgcgcacga cttccagcat acgttgccgt ggtgcgctta atcgcccttg caacatatcg
     5761 cagaccacca gcgcaccagc attaataaac ggattacgcg gaataccctg ttccatttcc
     5821 agttgcacta aagaattgaa cggtgatcca gacggatctt tgccgacgcg ttgccagatt
     5881 tcctcttcgg agtaatgacg catggcgaca acgagactca gcactttgga aatagactga
     5941 atggaaaaac gttcttgcgc gtctccggcc tgaaaaagct gtccgtcaac ggtacagata
     6001 gcaatgccca atcgggaacc gtctactgta gccagcgccg gaatataatc cgcgacttta
     6061 ccctgaccaa tgagcggccg cacttgccgc aagatgtttt ctaaaattgc attatccatg
     6121 gcgactgcca ctttctactc ctggaccgca ggtctgaaaa gacctgcgag tatatcagag
     6181 ctgaatatgt cgcgtcagat ccggtctttc cacaccgtct ggatattaca gaattcgtgt
     6241 aagccgaaat gggaaagctc acgaccaaag ccactctttt tcacgccacc aaaggccact
     6301 cgcgcgtcgc tggcacaata accattgata aacaccccac cgcattccag acgtgccgcc
     6361 atctgtctgg cctgtgtttc gtcggtggta aaaatggtcg ctgaaaggcc gaactcacta
     6421 tcattagcca gttccagcgc atgttctgca tctttcgcaa cggtgattgc cgcaacgggg
     6481 ccaaacattt cttcccgaaa cgcggtcatt tctggggtaa cattcgccag aaccgttggc
     6541 ggatagtagt tacctgcccc agccatcttt tccccgccca gtaacaaacg cgcaccctgc
     6601 gccagggttt tctctacctg atgatgcagc tcatcacgta aatcaaaacg agccattggt
     6661 ccgagagtgt tctcttcgtc acggggatcg cccattttca aggctgccgc agctgccaca
     6721 aaacgttcgg taaatgcaga agcaattccc tcttcgataa taaagcgttt tgccgctgca
     6781 catacctgtc cggtattctg ataacgtccg gctaccgccg ctttaaccgc cagttccaga
     6841 tcggcatcgt taagcacaat aaacggatcc gaaccgccca gttccagtac gcattttttc
     6901 agtgccgctc cagcctgtgc gccaatagcc gctccagcac gaacacttcc ggtcaccgtg
     6961 acagcagcaa tgcgcgagtc tttaatcatc tgactgacac cgtcgttgtc ggcattcagc
     7021 cagccatata cgccttgcgg gattcccgca tctttaaaca cctgggcaat gagctgagca
     7081 catcccatca cattcggcgc atgtttaagt aagtagccgt tacctgcaag aatgatggga
     7141 acagcgccac gcatcacctg ccataacgga aaattccacg gcataatcgc cagaatcgtc
     7201 cccaacggtc gatactcaat aaccgcctgc tgattttcca ccagtgtagg ttccgccttc
     7261 agcattgccg gaccatgttc tgcataccag tcacacaaat tcgccgattt cgccacttca
     7321 gcgcgcgcct ggttgattgg tttgcccatt tcgcgggtga tcatttgcgc catttcttcg
     7381 ctacgggcgc gcagagcctt accgatatca cgcatttttt cagcacgata atctatattt
     7441 gtctcgcgcc agtcgcgaaa gcctgctgcc gccagctcaa gtgcgttttc gatatcgtta
     7501 gcgccagccc acggcagcac agaaagttgt tcacccgtgg caggatttat tgaaattgca
     7561 tgagttgccg gagtaatggt catcggggta tctcctttat gagtcatggt atgaagatac
     7621 gcagatttac tcttgcttta aaatgaataa tattaagcca cttattcacg aatcgagaat
     7681 gttatggatc tgacccaact ggagatgttc aacgcggttg ccgaagctgg cagcattacc
     7741 caggctgcag caaaagtgca tcgcgtaccg tcgaatctca ctacccgtct acgccagctg
     7801 gaaacagaac tgggggtaga tctgtttatt cgcgagaatc agcgtttacg tctctctcct
     7861 gccgggcata actttttacg ctatagccaa caaattctcg cgttagtgga tgaagcgcgg
     7921 agcgttgtcg ctggcgatga gccgcaaggt ttattttctc ttggttcgct ggaaagcacc
     7981 gcagcagtgc gcattccggc cacgctggcg gaattcaacc atcgttatcc caaaattcag
     8041 ttttcacttt ccaccggccc ttccggcact atgctggaag gagtgctgga gggaaaactg
     8101 aatgcggcat ttattgatgg gcccattaac catactgcca ttgacgggat accggtatac
     8161 cgcgaggagc tgatgatcgt cacgccacaa ggtcatgcgc cagtaatccg cgccagtcag
     8221 gttaacggca gtaacattta tgccttccgc accaattgtt cgtatcgtcg ccacttcgag
     8281 agctggtttc atgctgacgg tgccgctccg ggaactattc acgagatgga gtcgtatcac
     8341 gggatgttgg cctgtgtggt cgcaggagca ggcattgcgc ttattccacg ctctatgctg
     8401 gaaagtatgc cggggcatca ccaggttgaa gcgtggccgt tagctgagca atggcgttgg
     8461 ttaacaacct ggctggtctg gcgtcgcggt gcgaaaacgc gtccgcttga ggcatttatt
     8521 caactactgg atgtgtctga ctcggcaaaa cagagttacc aatgaactat tttgatagtt
     8581 cttgcggcta atatgctcta tatagtgatg ttccgatgac ttatgactat atggggcaaa
     8641 tatggttacg ccagtaagca tcagcaatta catatctctt cctgatgatt ttcccgttcg
     8701 taatattgca ccacaggtaa aagaagtttt aaaggacttt attgatgcgc taagtacaat
     8761 aatatgtgat gaagagtggc gcacgtcttt aaacatcaac tcagccacaa aaaagatatt
     8821 taataacctt gataacttat catatattca gaggacttct ttccggggta acgacacgct
     8881 atacaatgaa aaggttcagt ttaaactcac ttatcccgtc aagaatggaa gacacaaaga
     8941 aaatattgaa tttcaggtag taataaattt aagccctatt tatttagata attttcgcca
     9001 tgatggagaa attaatattt tttgcgctcc caacccaaag cctgtcacta tggggcgcgt
     9061 atttcagacc ggcgtcgagc gtgtgctctt tctttttctg aatgatttta ttgaacaatt
     9121 tccaatgttc aaccctggcg ttccgatcaa aatagcgcat acaccacata ttgaacccct
     9181 gcctccggat caccataccg ctgcagatta cttacgccag tttgatttgc ttgtcctgaa
     9241 ttttatctct cgcggtaatt ttgtcatact cccccgatta tggaataact ctgaggttca
     9301 cagatggttc gtcaataaag atcctaactt gatcaccgcc attctcgaca taacggacag
     9361 cgaattaaaa gaggatttat tgcaaagcct gatggattca ttaggttcta acaaacatgt
     9421 actacccgaa gtctgcatct gctttttatt ccttttagca gaacaagaat ctcctcattt
     9481 tcaggacttg tttttatttt tcgccaatat gttattgcac tatcaccaat ttatgaatcc
     9541 caatgaaagt gatttgaatg acgtgttgat gccagcatca ttaagtgatg ataaaattat
     9601 caagcatatg gcgcgcagga ccctcgaact gtttgtaaaa aatgaaacac cgccaaaagt
     9661 tactcacgaa gacctggtga aaaacaggcc tcgctcccct gtcagaccac ctatacccgc
     9721 aacctccaaa acgccagacc tccctgaacg tcattaaacc gtgatgttac cgactctctg
     9781 acgcggaaca gaatcagcgt cagagaaacg gaaaacgcga tccagatcac aaatacattg
     9841 tattcacatc attaaccgtt ttaagatcat ttcatcactt tttcgcaact cacccgataa
     9901 tctgttatga caacaaacac tgtttcccgc aaagtggcgt ggctacgggt cgttacgctg
     9961 gcagtcgccg ccttcatctt caacaccacc gaatttgtcc ctgttggcct gctctctgac
    10021 attgcgcaaa gttttcacat gcaaaccgct caggtcggca tcatgttgac catttacgca
    10081 tgggtagtag cgctaatgtc attgcctttt atgttaatga ccagtcaggt tgaacggcgc
    10141 aaattactga tctgcctgtt tgtggtgttt attgccagcc acgtactgtc gtttttatcg
    10201 tggagcttta ccgttctggt gatcagtcgc attggtgtgg cttttgcaca tgcgattttc
    10261 tggtcgatta cggcgtctct ggcgatccgt atggctccgg ccgggaagcg ggcacaggcc
    10321 ttgagtttaa ttgccaccgg tacagcactg gcgatggtct taggtttacc tctcgggcgc
    10381 attgtggggc agtatttcgg ttggcgaatg accttcttcg cgattggtat tggggcgctt
    10441 atcacccttt tgtgcctgat taagttactt cccttactgc ccagtgagca ttccggttca
    10501 ttgaaaagcc tcccgctatt gttccgccgc ccggcattga tgagcattta tttgttaact
    10561 gtggtggttg tcaccgccca ttacacggca tacagctata tcgagccttt tgtgcaaaac
    10621 attgcgggat tcagcgccaa ctttgccacg gcattactgt tattactcgg tggtgcgggc
    10681 attattggca gcgtgatttt cggtaaactg ggtaatcagt atgcgtctgc gttggtgagt
    10741 acggcgattg cgctgttgct ggtgtgtctg gcactgctgc tacctgcggc gaacagtgaa
    10801 atacacctcg gggtgctgag tattttctgg gggatcgcga tgatgatcat cgggcttggt
    10861 atgcaggtta aagtgctggc gctggcacca gatgctaccg acgtcgcgat ggcgctattc
    10921 tccggcatat ttaatattgg aatcggggcg ggtgcgttgg taggtaatca ggtgagtttg
    10981 cactggtcaa tgtcgatgat tggttatgtg ggcgcggtgc ctgcttttgc cgcgttaata
    11041 tggtcaatca ttatatttcg ccgctggcca gtgacactcg aagaacagac gcaatagttg
    11101 aaaggcccat tcgggccttt tttaatggta cgttttaatg atttccagga tgccgttaat
    11161 aataaactgc acacccatac ataccagcag gaatcccatc agacgggaga tcgcttcaat
    11221 gccacccttg cccaccagcc gcataattgc gccggagctg cgtaggcttc cccacaaaat
    11281 aaccgccacc aggaaaaaga tcagcggcgg cgcaaccatc agtacccaat cagcgaaggt
    11341 tgaactctga cgcactgtgg acgccgagct aataatcatc gctatggttc ccggaccagc
    11401 ggtacttggc attgctagcg gcacaaaggc aatattggca ctgggttcat cttccagctc
    11461 ttccgacttg cttttcgcct ccggtgaatc aatcgctttc tgttgcggaa agagcatccg
    11521 aaaaccgata aacgcgacga ttaagccgcc tgcaattcgc agaccgggaa tcgaaatgcc
    11581 aaatgtatcc atcaccagtt gcccggcgta atacgccacc atcatgatgg caaatacgta
    11641 caccgaggcc atcaacgact gacgattacg ttcggcactg ttcatgttgc ctgccaggcc
    11701 aagaaatagc gcgacagttg ttaatgggtt agctaacggc agcaacacca ccagccccag
    11761 gccaattgct ttaaacaaat ctaacattgg tggttgttat cctgtgtatc tgggttatca
    11821 gcgaaaagta taaggggtaa acaaggataa agtgtcactc tttagctaac cttgcatcgc
    11881 attgaccaaa acttgaaccg atttagcaaa acgtggcatc ggtcaattca ttcatttgac
    11941 ttatacttgc ctgggcaata ttatcctctg caactaatta cttgccaggg caactaatgt
    12001 gaaaagtacc agcgatctgt tcaatgaaat tattccattg ggtcgcttaa tccatatggt
    12061 taatcagaag aaagatcgtc tgcttaacga gtatctgtct ccgctggata ttaccgcggc
    12121 acagtttaag gtgctctgct ctatccgctg cgcggcgtgt attactccgg ttgaactgaa
    12181 aaaagtgttg tcggtcgacc tgggagcact gacccgtatg ctggatcgcc tggtctgtaa
    12241 aggctgggtt gaaaggttgc cgaacccgaa tgataagcgc ggcgtactgg taaaacttac
    12301 caccagcggc gcggcaatat gcgaacaatg ccatcaatta gttggccagg acctgcatca
    12361 agaattaaca aaaaacctga cggcggacga agttgcaaca cttgagcatt tgcttaagaa
    12421 agtcctgccg taaacaaaaa agaggtatga cgatgtccag acgcaatact gacgctatta
    12481 ccattcatag cattttggac tggatcgagg acaacctgga atcgccactg tcactggaga
    12541 aagtgtcaga gcgttcgggt tactccaaat ggcacctgca acggatgttt aaaaaagaaa
    12601 ccggtcattc attaggccaa tacatccgca gccgtaagat gacggaaatc gcgcaaaagc
    12661 tgaaggaaag taacgagccg atactctatc tggcagaacg atatggcttc gagtcgcaac
    12721 aaactctgac ccgaaccttc aaaaattact ttgatgttcc gccgcataaa taccggatga
    12781 ccaatatgca gggcgaatcg cgctttttac atccattaaa tcattacaat agctagttga
    12841 taacgtgaca acgtcactga ggcaatcatg aaaccacttt catccgcaat agcagctgcg
    12901 cttattctct tttccgcgca gggcgttgcg gaacaaacca cgcagccagt tgttacttct
    12961 tgtgccaatg tcgtggttgt tcccccatcg caggaacaac caccgtttga tttaaatcac
    13021 atgggtactg gcagtgataa gtcggatgcg ctcggcgtgc cctattataa tcaacacgct
    13081 atgtagttat tgtcaggctc tgatcacaga gcctgacatc tccgtaatat cttcctcaag
    13141 tctcaaatat tttcgccatt gttggcgatg agatcccgat accagtaaaa cgacgcttta
    13201 cgtatccgtt ccagcgttcc cttcccttcg ttgtctttat cgacataaat cattccgtag
    13261 cgctttttca tttcccctgt tccggcagaa accaggtcaa tgcagcccca cggggtgtag
    13321 ccaattaagt caacaccatc ttcaactacg gcttttttca tttcccgaat atgggaagca
    13381 aagtaatcaa tgcgatagtg atcgttcacc gtgccgtcag cttgtctctg gtcaaccgca
    13441 ccaaatccat tttcgacaat aaacagcggc aactggaaat gatcccagaa ccagttgagt
    13501 gaataacgta gccctgccgg atcaatttgc catccccatt cggatgtatc gatataaggg
    13561 ttgctgacca aatctcgtgg ttcaacataa tccagttgcg ggttatcggg agaaaattgt
    13621 gtcgtaaaag acatgtagta gctaaaaccg ataaagtcga cacatccact ggcaagaatc
    13681 gcgttatctt ctggtgtgat atcgaggttg aatcctttcc tggcaaagta attcagcata
    13741 tgttgcggat aatatccacg agcatgaaca tcagtaaacc agtaacgacg atgcatcgct
    13801 ttcgtggcca tcatcatatc gttgggtgca cacgtcagag gatagatggg gcacatggcg
    13861 atcatacagc caatgttaaa ttcaggattg atcgattttc cagtctgtac cgccagggca
    13921 ctggcaacta actcgtaatg taccgcctgg tacattattt gttcgcgctc attgatatct
    13981 tcctctggcg aatacaagat accggaatta gtaaatggac acaggctttc gctgaaattc
    14041 acctgattat tgatttcgtt aaacgtcatc cagtacttta ctttttcttt atagcgcgtg
    14101 aagaccgttg atgcgaagcg gataaaaaag tcgatcagct tacggtttcg ccagccacca
    14161 tattttgtca ccagatgata aggcatctca aaatgcgaaa gcgtcaccac aggttccatt
    14221 ccttgcttca ggcattcatc gaacagatca tcataaaatt gtaaaccctc ttcattcggc
    14281 tcctgttcgt caccttgcgg aaagattcgt gtccaggcaa tggaagttcg aaagcatttg
    14341 aatcccatct cggcaaataa ctgaatatct gttttatagc gatgataaaa atcaattgct
    14401 tcatgattgg gataattaag cccgtcgata acgccttctg tcacttcacg cggcaccccg
    14461 tgagcgccag cagtcatcac atcagcgata ctgatgcctt ttcctccttc attccagcca
    14521 ccttccaact gatgcgcggc taccgcgcca ccccataaaa aacctttttt aaatcctgac
    14581 atacacaact ccttaactaa aatagattac caccagactt c
//
LOCUS       DAZPEP010000102        14524 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZPEP010000102 DAZPEP010000000
VERSION     DAZPEP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.102"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="ACHUT9_002930"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="ACHUT9_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFR1349351.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUT9_002931"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="ACHUT9_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFR1349352.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUT9_002932"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="ACHUT9_002932"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFR1349353.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUT9_002933"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="ACHUT9_002933"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFR1349354.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            complement(3836..5053)
                     /gene="caiB"
                     /locus_tag="ACHUT9_002934"
                     /pseudo
     CDS             complement(3836..5053)
                     /gene="caiB"
                     /locus_tag="ACHUT9_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
     gene            complement(5182..6324)
                     /gene="caiA"
                     /locus_tag="ACHUT9_002935"
     CDS             complement(5182..6324)
                     /gene="caiA"
                     /locus_tag="ACHUT9_002935"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFR1349355.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            complement(6355..7869)
                     /gene="caiT"
                     /locus_tag="ACHUT9_002936"
     CDS             complement(6355..7869)
                     /gene="caiT"
                     /locus_tag="ACHUT9_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFR1349356.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            8341..9111
                     /locus_tag="ACHUT9_002937"
     CDS             8341..9111
                     /locus_tag="ACHUT9_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFR1349357.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            9126..10067
                     /locus_tag="ACHUT9_002938"
     CDS             9126..10067
                     /locus_tag="ACHUT9_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFR1349358.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            10118..11404
                     /locus_tag="ACHUT9_002939"
     CDS             10118..11404
                     /locus_tag="ACHUT9_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1349359.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            11401..11688
                     /gene="fixX"
                     /locus_tag="ACHUT9_002940"
     CDS             11401..11688
                     /gene="fixX"
                     /locus_tag="ACHUT9_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFR1349360.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            11747..13078
                     /locus_tag="ACHUT9_002941"
                     /pseudo
     CDS             11747..13078
                     /locus_tag="ACHUT9_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13186..13716
                     /gene="kefF"
                     /locus_tag="ACHUT9_002942"
     CDS             13186..13716
                     /gene="kefF"
                     /locus_tag="ACHUT9_002942"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFR1349361.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            13709..>14524
                     /locus_tag="ACHUT9_002943"
     CDS             13709..>14524
                     /locus_tag="ACHUT9_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HFR1349362.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLL"
BASE COUNT         3329 a         3741 c         3887 g         3567 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg ccaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggccagacct ttgctgacca actcctgaat gtcgttttcg ctgtagccga
     3901 tatttttcaa aatggcagcc gtgtccatgc catgtgaggg cattccgcgc cagatttgtc
     3961 cggggttatt tttgaatttc ggcatgatgt tcggcccttt gcaggtgcga ccatccatcg
     4021 tttgccactg agtgattgat tcacgggcaa catactgtgg attgctttcc agttccggta
     4081 cggtcagtac tttggcacag gcgatattca gttcggcaaa gcgttcttta acttctgcga
     4141 tggtatgtgc cgccagccag gcatcgagtt tttcttctac cagtgggccg taagggcatt
     4201 cgatacggtg gataagctga gtgccttccg ggatttccgg cgtaccgagt agatgtgcga
     4261 ggccaatatc tttaaagcac tcttcaattt gggtaatgcc caccagttcc atcacgatgt
     4321 agccgtcggc gcatttatac agaccgcaac cggcgtagta gggatcttta cctttgctca
     4381 tgcgcgggca catttcgccg ccgttgaagt aatccatcat gaagtactgg cccatacgca
     4441 gcatcacttc atacatggcg atgtcgatac tttcgccttt accggtttca cgcactttat
     4501 gcagtgctgc cagcgccgcc gtggtggcgg tcaggccaga aaagtaatcg gcggtatacg
     4561 ggaaggcagg cattggctgg tcaacatcac cgttctgaat caggtaacca ctgaaggcct
     4621 gggcgatggt gttataggcc ggaagattgg tgtactcctc ggtgccgtac tgaccaaaac
     4681 cggacaggtg agcgataacc agtttcgggt tgtgctgcca cagtacttca tcggtaatgc
     4741 cacgacgggc aaaggccgga cctttgctgg cttcgatgaa gatatcggtg gtttccatta
     4801 atttcagaaa cgcttcgcgg ccttcatctt tgaaaatatt taacgacagc gcgtgcaaat
     4861 tgcggcggga gagctgcggg tagttcggtt gaacgcgaat ggtgtcggcc taggcgacgt
     4921 tctcgatcca gataacttcc gcaccccatt ctgcgaacat ttgcccggcg aacggcccgg
     4981 cgatttcgat accggagaag acaacgcgca atccggccaa cggcccgaat ttcggcatgg
     5041 gtagatgatc cattattagc tcctgaaaaa tttatgtagc gcatgactgc cggatgcggc
     5101 gtaaacgctt tatccggcct acattcgtgc tcccgtaggc ctgataagac gcattagcgt
     5161 cgcatcaggc agcgcacgga cttagcggta ttgcttcagc accgcgcgac ccagcgtcag
     5221 gatctgcatt tcgtcagatc ccccggagac gcggtctaca cgcagatcac gccagaagcg
     5281 gctgatgcgg tggttgcccg caatcccgac accgcccagc acctgcattg cgctatccac
     5341 aacttcaaat gccgcattgg cgcagaagta tttgcacatc gctgcatcgc cagaggtgat
     5401 ggtgccgttg tctgctttcc acgctgcttc atacagcatg tttttcatgg agtttaattt
     5461 gatcgccatg tgggcgaatt tttcctgaat caactggaaa cgaccaatag cctcgccaaa
     5521 ctgcacgcgc tgattggcgt agcgcgccgc atcttcaaaa gcgcacatcg ccgtaccgta
     5581 gttggtgagg gctaccagga aacgttcatg gtcgaactct tctttgacgc ggttaaagcc
     5641 gttaccttcc cgaccgaaca tgtctttctc gtccagttcc acgtcgtcaa aggtgatttc
     5701 acagcagcta tccatacgca gaccgagctt ttcaagtttg gtcactttga tgcccggttt
     5761 gctcatatca acaaaccatt cggtgtagac aggtttgtcc ggagaagccc cgtcgcgcgc
     5821 catcaccacg atgtacgggg tgtaggcgct gctggtaata aaacacttac taccattaag
     5881 ataaatctta ccatttctac gggtataagt cgttttcagg ctacccacgt cggagcccgc
     5941 gcccggttcg gtaatcgctg agttccacat ctgcttaccg gtgccgcgga aagccataat
     6001 tttgtcgatc tgctcttgtg tgccttcgcg caggaaggtg ttgaacccgc ccggcaactg
     6061 gtacagcaca taggttggtg cccccagacg tcccagctcc atccacacgg cggcgagagt
     6121 aacaaacccc gcgtccagac caccgtgctc ttcagggatc agcagactgt cgatacccat
     6181 atccgccagt gctttgacaa aacgttccgg gtagacgctg tcacggtcgc actcggcaaa
     6241 ataggcctcc cagttttcgc tggccatcag ttcgcggata ccggcgacaa acagttcctg
     6301 ctcatcattt aaattaaaat ccatctttca acctcttgat attttggggg ttaattaatc
     6361 tttccagttc tgtttcgcgt ctttaataaa ggagagcgtc accataatgt tgacgaagaa
     6421 cagcgggcat cctccggcga taatggcggt ttgaatcggt ttcaggccgc cgagcgccag
     6481 cagaacaata ccgataatgc caaccagaat tgaccaaccg atacgcacca gcagaggtgg
     6541 ttcttcacca tcgcgtactt cgcggcaagt ggacatcgcc agggtataag agcaggcgtt
     6601 aaccagcgta acggtggcaa taaagcagag gatgaagaag ccccacatgg tggcggtgct
     6661 gagtggcaga gcggcccagg tttcaatgat ggcgcgcgcc acaccgtact gttcgatcag
     6721 atttggaatg ttgatgatgt ttttatctat caacagcaga gtgttactac cgagtacagt
     6781 ccacaggatc caggttgacg ctgtcagccc cagcaccatg ccgaagcaca gttcacgcac
     6841 agtacgacca cgggagatgc gggcgaggaa gatactcatc tggatagcat aaatcaccca
     6901 ccatgcccag tagaacacgg tccagccctg cgggaagccg cctttagcga tgggatcggt
     6961 atagaacaac atgcgcggca gatacatcag caacatcccc accgaatcgg tgaagtagtt
     7021 catgatgaag ctggcaccgc tgacaatgaa cacccaaccc agcatcagga agctcaggta
     7081 actacgcacg tcactggcga tacgtacccc tttttgcaga ccgcaagcga cgcaaatggc
     7141 gttgaggata atccagcagg taatgatgat agcgtccagt tgcagggtat gcggaatgcc
     7201 aaacaaccat tgcatacact cggtcaccag cggcgtggca aggcccagac tggtacccat
     7261 cgcgaagatc aaggcgacga gatagaagtt gtcgacgata gtgccgaaca accctttggc
     7321 gtgtttttca cctaccagcg gcaccagtgt cgagctgggg cgaatcactt ccattttgcg
     7381 gacaaagaag aagtaagcga aggcgactga aaggaagctg taagtggccc acggcagagg
     7441 tccccagtgg aacaagctgt aagccagccc caactctttc gcccctgtcg agttcggttc
     7501 taagccaaac ggcggggtgg agatgtagta gtagatctca atcgatcccc agaacagtac
     7561 ggcagcagac gtacaggagg cgaacatcat aaagatccaa ctggcggtac taaattcagg
     7621 tggttcgtta cctaaacgct ttttggcata cgggccaaac accagccaga accaaccgaa
     7681 aagcatcacc accatatacc attcaaatgc ccatccccat acattggtga cgtaactgaa
     7741 tacagcatta ataacgacat tcgctgcatc cagatctctg actgtaagcc aacaaagtat
     7801 gccgacgatt attaacggcg gaaagaaaac cttcggttct attcccgttt ttctcttttc
     7861 attcttcatg agttaattcc actgtgaaaa cgaatattta ttttgcgttc ccgtttgttt
     7921 tatttttgtt aacatttgat ataattatta ttaacctcgt ggacgcgtta atggctaact
     7981 cataatgggt attcaataag ctgcattctg tgattgttat cacatttttt tcgggtgaat
     8041 agagggcgct tttctcgtta attttgatta ttaatcagtt tgttatgttg tgttgtgagt
     8101 aaaaaaatag catctgactt tcaatattgg tgatccataa agcaatattg aaaatttctt
     8161 ttttctacgc catgttttca atattggtga ggaacttaac aatattgaaa gttggattta
     8221 tctgcgtgtg acattttcaa tattggtgat taaagtttta tttccaaatt aaagggcgtg
     8281 atatctgtaa ttaacaccac cgatatgaac gacgtttcct tcatgatttc tggagatgca
     8341 atgaagatta ttacttgcta taagtgcgtg cctgatgaac aggatattgc ggtcaataat
     8401 gctgatggtt cattagactt cagcaaagcc gatgccaaaa taagccaata cgatctgaac
     8461 gctattgaag cggcttgcca gctaaagcaa caggcagcag aggcgcaggt gacggcctta
     8521 agtgtgggcg gtaaagccct gaccaacgcc aaagggcgta aagatgtact atcgcgcggc
     8581 ccggatgaac tgattgtggt gattgatgac cagttcgagc aggcactgcc gcaacaaacg
     8641 gcgagcgcac tggctgcagc cgcccagaaa gcaggctttg atttgatcct ctgtggcgat
     8701 ggttcttccg acctttatgc ccagcaggtt ggtctgctgg tgggcgaaat cctcaatatt
     8761 ccggcagtta acggcgtcag caaaattatc tccctgacgg cagataccct caccgttgag
     8821 cgcgaactgg aagatgaaac cgaaacctta agcattccgc tgcctgcggt tgttgctgtt
     8881 tccactgata tcaattcccc acaaattcct tcgatgaaag ccattctcgg cgcggcgaaa
     8941 aagcccgtcc aggtatggtc ggcggcggat attggtttta acgcagaggc agcctggtca
     9001 gaacaacagg ttgccgcgcc gaaacagcgc gaacgtcagc gcatcgtgat tgaaggcgac
     9061 ggcgaagaac agatcgccgc atttgctgaa aatcttcgca aagtcattta attacagggg
     9121 atgctatgaa cacgttttct caagtctggg tattcagcga taccccttct cgtctgccgg
     9181 aactgatgaa cggtgcgcag gctttagcta atcaaatcaa cacctttgtc ctcaatgatg
     9241 ccgacggcgc acaggcaatc cagctcggcg ctaatcatgt ctggaaatta aacggcaaac
     9301 cggacgatcg gatgatcgaa gattacgccg gtgtcatggc tgacactatt cgccagcacg
     9361 gcgcagacgg cctggtgctg ctgccaaaca cccgtcgcgg caaattactg gcggcaaaac
     9421 tgggttatcg ccttaaagcg gcggtgtcta acgatgccag caccgtcagc gtacaggacg
     9481 gtaaagcaac agtgaaacat atggtttacg gtggtctggc gattggcgaa gaacgcattg
     9541 ccacgccgta tgcggtactg accatcagca gcggcacgtt cgatgcggct cagccagacg
     9601 cgtcacgcac tggcgaaacg cacaccgtgg agtggcaggc tccggctgtg gcgattaccc
     9661 gcacggcaac ccaggcgcgc cagagcaaca gcgtcgatct cgacaaagcc cgtctggtgg
     9721 tcagcgtcgg tcgcggtatt ggcagcaaag agaacattgc gctggcagaa cagctttgca
     9781 aggcgatagg tgcggagttg gcctgttctc gtccggtggc ggaaaacgaa aaatggatgg
     9841 agcacgaacg ctatgtcggt atctccaacc tgatgctgaa acctgaactg tacctggcgg
     9901 tggggatctc cgggcagatc cagcacatgg ttggtgctaa tgcatcgcaa accattttcg
     9961 ccatcaataa agataaaaat gcgccgatct tccagtacgc ggattacggc attgttggtg
    10021 acgccgtgaa gatccttccg gcgctgaccg cagctttagc gcgttgatcc actctggcag
    10081 ggctgcattt tggccctgcc gctgacaggg agctcttatg tccgaagata tctttgacgc
    10141 catcatcgtc ggtgctgggc ttgccggttc ggttgccgca ctggtgctcg cccgcgaagg
    10201 ggcgcaagtg ttagttatcg agcgtggcaa ttccgcaggt gccaagaacg tcaccggcgg
    10261 gcgtctctat gcccacagcc tggaacacat tattcctggt ttcgccgaat ccgccccggt
    10321 agaacgcctg atcacccatg aaaaactcgc gtttatgacg gaaaagtcag cgatgactat
    10381 ggactactgc aacggtgacg aaacctcgcc atcccagcgt tcttactccg ttttgcgcag
    10441 taaatttgat gcctggctga tggagcaggc cgaagaagcg ggcgcgcagt taattaccgg
    10501 gatccgcgtc gataacctcg tacagcgcga tggcaaagtc gtcggtgtag aagccgatgg
    10561 cgatgtgatt gaagcgaaaa cggtgatcct tgctgatgga gtgaactcca tccttgccga
    10621 aaagctgggg atggcaaaac gcgttaaacc gacggatgtg gcggttggcg tgaaggaact
    10681 gatcgagtta ccgaagtcgg taatcgaaga ccgttttcag ttgcagggta atcagggcgc
    10741 ggcttgtctg tttgcgggat cacccaccga tggcctgatg ggcggcggct tcctttatac
    10801 caatgaaaat accctgtcgc tggggctggt ttgtggtctg catcatctgc atgacgcaaa
    10861 aaaatcggtg ccgcaaatgc tggaagattt caaacagcat ccggcagtgg caccgctgat
    10921 cgcgggcggc aagctggtgg aatattccgc tcacgtagtg ccggaagcag gcatcaacat
    10981 gctgccggag ttggttggtg acggcgtatt gattgccggt gatgccgccg gaatgtgtat
    11041 gaacctcggt tttaccattc gcggtatgga tctggcgatt gccgccgggg aagccgcagc
    11101 aaaaaccgtg ctttcagcga tgaaaagcga cgatttcagt aagcaaaaac tggcggaata
    11161 tcgtcagcat cttgagagtg gcccgctgcg cgatatgcgt atgtaccaga aactaccggc
    11221 cttccttgat aacccacgca tgtttagcgg ctacccggaa ctggcggtgg gcgtggcgcg
    11281 tgacctgttc accattgacg gcagtgcgcc ggaactgatg cgcaagaaaa tcctccgcca
    11341 cggcaagaaa gtgggcttca tcaatctgat caaggatggc atgaaaggag tgaccgtttt
    11401 atgacttctc ccgtcaatgt ggacgtcaaa ctgggcgtca ataaattcaa tgtcgatgaa
    11461 gagcatccgc acattgttgt gaaggccgat gccgataaac aggccctgga gctgctggtg
    11521 aaagcgtgcc ccgcaggtct gtacaagaag caggatgacg gcagtgtgcg cttcgattac
    11581 gccggatgtc tggagtgcgg cacctgtcgc attctggggc tggggtcggc gctggaacag
    11641 tgggaatacc cgcgcggcac ctttggtgtg gagttccgtt acggctgatg ttggtttgat
    11701 acgtaacgcc gtactgactc tcattgcaaa aaaacaggaa taacccatgc aaccgtccag
    11761 aaactttgac gatctcaaat tctcctcaat tcaccgccgc attttgctgt ggggaagcgg
    11821 tggtccgttt ctggatggtt atgtactggt aatgattggc gtggcgctgg agcaactgac
    11881 tccggcgcta aaactggacg ctgactggat tggcttgttg ggcgcgggaa cgctcgccgg
    11941 gctgttcgtt ggcacatcgc tgtttggcta tatttccgat aaagtaggac ggcgcaaaat
    12001 gttcctcatt gatatcatcg ccatcggcgt gatatcggtg gcgacgatgt ttgtttcatc
    12061 ccccgtcgaa ctgttggtga tgcgggtact tatcggcatt gtcatcggtg cagattatcc
    12121 catcgccacc tcaatgatca ccgagttctc cagtacccgt cagcgggcat tttccatcag
    12181 ctttatcgcc gccatgtggt atgtcggcgc gacctgcgcc gatctggtcg gctactggct
    12241 ttatgatgtg gaaggcggct ggcgctggat gctgggtagc gcggcgatcc cctgtttgtt
    12301 gattttgatt ggtcgattcg aactgcctga atctccccgc tggttattac gcaaagggcg
    12361 agtaaaagag tgcgaagaga tgatgataaa actgtttggc gaaccggtgg ctttcgatga
    12421 agagcagccg tagcaaaccc gttttcgcga tctgtttaat cgccgccatt ttccttttgt
    12481 tctgtttgtt gccgccatct ggacctgcca ggtgatccca atgttcgcca tttacacctt
    12541 tggcccgcaa atcgttggtt tgttgggatt gggtgttggc aaaaacgcgg cgctggggaa
    12601 cgtggtgatt agcctgttct ttatgctcgg ctgtattccg ccgatgctgt ggttaaacac
    12661 tgccggacgg cgtccattgt tgattggcag ctttgccatg atgacgctgg cgctggcggt
    12721 tttggggctg atcccggata tggggatctg gctggtagtg atggcctttg cggtgtatgc
    12781 ctttttctct ggcgggccgg gtaatttgca gtggctctat cctaatgaac tcttcccgac
    12841 agatatccgc gcctctgccg tgggcgtgat tatgtcctta agccgtattg gcaccattgt
    12901 ttcgacctgg gcactgccga tctttatcaa taattacggt atcagtaaca cgatgctaat
    12961 ggaggcgggt atctcgctgt ttggcttgtt gatttccgta gcgtttgccc cggagactcg
    13021 agggatgtca ctggcgcaaa ccagcaatat gacgatccgc gggcagagaa tggggtaaat
    13081 tgttcagatt tctctctttt ctgaatcaat attattgact ataagccgcg tgaatatatg
    13141 actacacttt gtgggaaaac aaaggcgtaa tcacgcgggc tacctatgat tcttataatt
    13201 tatgcgcatc cgtatccgca tcattcccat gcgaataaac ggatgcttga acaggcaagg
    13261 acgctggaag gcgtcgaaat tcgctctctt tatcaactct atcctgactt caatatcgat
    13321 attgccgccg agcaggaggc gctgtctcgc gccgatctga tcgtctggca gcatccgatg
    13381 cagtggtaca gcattcctcc gcttcttaaa ctttggatcg ataaagtttt gtcgcacggc
    13441 tgggcttacg gtcacggcgg caaggcgctg catggcaaat atttgctgtg ggcggtgacg
    13501 accggcggcg gggaaagcca ttttgaaatt ggtgcgcatc cgggctttga tgtgctgtcg
    13561 cagccgctac aggcgacggc aatctactgc gggctgaact ggctgccacc gtttgccatg
    13621 cactgcacct ttatttgtga cgacgaaacc ctcgaagggc aggcgcgtca ctataagcaa
    13681 cgtctgctgg aatggcagga ggcccatcat ggatagccat acgctgattc aggcgctgat
    13741 ttatctcggt tcggcagcgc tgattgtacc cattgcggta cgtcttggtc tgggatcggt
    13801 acttggctac ctgatcgccg gctgcattat tggcccgtgg gggctgcgac tggtgaccga
    13861 tgccgaatct attctgcact ttgccgagat tggggtggtg ctgatgctgt ttattatcgg
    13921 cctcgaactc gatccacaaa ggctgtggaa gctgcgtgcg gcagtgttcg gcggtggcgc
    13981 attgcagatg gtgatttgcg gcggcctgct ggggctgttc tgcatgttac ttgggctgca
    14041 ctggcaggtc gcggaattga tcggcatgac gctggcgctc tcctctacgg cgattgccat
    14101 gcaggcgatg aatgaacgca atctgatggt gacgcaaatg ggtcgcagtg cctttgcggt
    14161 gctgctgttc caggatatcg cggcgatccc gctggtggcg atgattccgc tactggcaac
    14221 gagcagtgcc tcgacgacga tgggcgcatt tgctctctcg gcgttaaaag tggcgggtgc
    14281 gctggtgctg gtggtattgc tggggcgcta tgtcacgcgt ccggcgctgc gttttgtagc
    14341 ccgctctggc ttgcgggaag tgtttagtgc cgtggcgtta ttcctcgtgt ttggctttgg
    14401 tttgctgctg gaagaggtcg gcttgtcgat ggcgatgggc gcgtttctgg cgggcgtact
    14461 gctggcaagc tcggaatacc gtcatgcgct ggagagcgat atcgaaccat ttaaaggttt
    14521 gctg
//
LOCUS       DAZPEP010000103        14368 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZPEP010000103 DAZPEP010000000
VERSION     DAZPEP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14368
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.103"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..696)
                     /locus_tag="ACHUT9_002944"
     CDS             complement(<1..696)
                     /locus_tag="ACHUT9_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFR1349363.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="ACHUT9_002945"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="ACHUT9_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFR1349364.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUT9_002946"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="ACHUT9_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFR1349365.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUT9_002947"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="ACHUT9_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFR1349366.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUT9_002948"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="ACHUT9_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFR1349367.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUT9_002949"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="ACHUT9_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFR1349368.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUT9_002950"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="ACHUT9_002950"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFR1349369.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUT9_002951"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="ACHUT9_002951"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFR1349370.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUT9_002952"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="ACHUT9_002952"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFR1349371.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="ACHUT9_002953"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="ACHUT9_002953"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFR1349372.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="ACHUT9_002954"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="ACHUT9_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFR1349373.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUT9_002955"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="ACHUT9_002955"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFR1349374.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUT9_002956"
     CDS             10119..10499
                     /gene="acpS"
                     /locus_tag="ACHUT9_002956"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFR1349375.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUT9_002957"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="ACHUT9_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFR1349376.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUT9_002958"
     CDS             complement(10995..11255)
                     /gene="yfhL"
                     /locus_tag="ACHUT9_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFR1349377.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            11470..12660
                     /locus_tag="ACHUT9_002959"
                     /pseudo
     CDS             11470..12660
                     /locus_tag="ACHUT9_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(12845..14032)
                     /locus_tag="ACHUT9_002960"
     CDS             complement(12845..14032)
                     /locus_tag="ACHUT9_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFR1349378.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(14032..>14368)
                     /locus_tag="ACHUT9_002961"
     CDS             complement(14032..>14368)
                     /locus_tag="ACHUT9_002961"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349379.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT         3622 a         3609 c         3706 g         3431 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggccc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccacg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagcct ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 tctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggcttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcaagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tggcgttaag gcttcccagt aatataatta atactctact
    10981 tccagagtag aatattaaat tttatccgcg tggtgcatca gcacaaattt atcccacaac
    11041 tgttcttctg tctcgacatg cgccggatct ttcacaatag tattggggat cgggcacacc
    11101 ttctggcagg ttggtgtctc gtagtgccct acgcattcgg tacacttatc gctgttaatc
    11161 tcgtagatat gatctcccat tgaaatcgcc tcattcgggc attcgggttc acacatatca
    11221 caattaatac agcgtttagt aattagtaaa gacatttcaa tggattaccg ttaaatcatt
    11281 ttaaaatcag taagttgtat cgagtttgta tgctttactg tcattaactt actgtatgtt
    11341 gatccagtgt atttaacctt gataaactca gtccagcaac acaaaaccgc aacacattgc
    11401 attttgtccc gtagaaaaga cttgtatgtg tgagcttgtt ttctgcgcct acgcagataa
    11461 ggattgagaa tgccgcgcac tgtaacacat aatccggata gccccaataa tgacgatgtt
    11521 ttagccgcat ctgaaaaatg ggacgcctgt aaacccccct ataccagcgc acacatgaaa
    11581 atctgtgttg ctgccgccaa aatcatcctc gctgcttccg gcgtggctcg ccgttccaaa
    11641 tacgaaaaag agaactatct ccgtatcgat ttcagcaaag ccggtaaggt tacattttac
    11701 gccgagtttc caaaaaagat gggcctcaaa ggtaaaaagc tcggcgagtg gccggagctc
    11761 gctatccagc tggcgcgcga aaaagcacta ggtatggctg acggtggact gcgggcagag
    11821 tccgtacatg cagcgctgga aatgtaccgg gatgacctca aagccaaagt agcccggcag
    11881 aagctgagcc cggacagttt cacaacctac ggggtgcgta tcgaccggat taaagcaacg
    11941 ttcggcgagc gtgaggtgtt cagcgacgta acatacaatc ggctggtgga agtgctggac
    12001 gagtggatcg ccactcgctc gaacaataac gccctggagt tgtttgccga gctccgtcgg
    12061 ttctggaagt tctgcgcacc tactctttgc aacggccgca atgttgccgc cagtctgcca
    12121 gatgattatg tttcctcccg cgtacagaaa cctaccccca cacggctttt taccgatatt
    12181 gaatcaatcg cccgactctg gctcaatgtt gctgcctgca cctctgtaca ccagaagaat
    12241 gctgttcgct tcatgatcat cactggtgtt cgtccgatta atgtccataa cctgcgctgg
    12301 gactacgttt acgaggaggc tggtgaaatt gtttatccgg aaggggttat cggcatgcga
    12361 ggggctatga aaacacaaaa ggctttccgc ctgccgataa cgcctgagat ccggcggatt
    12421 atcgacgagc agaaagcctg gcgtgattca gttcctgagt gcaacaggga ttatgtattt
    12481 ttgcagccac gtgatccaat gcagccattt tcaaaacgat cactggataa gctggtgaaa
    12541 acatacagcc cggacggggc tgtaaaagga ataaaacatg atgggactgt taaagggaaa
    12601 gacggtgcat ttaatacgat gtgccgtaaa ttccttaaga gcaatgttat tgccttgatc
    12661 gataggaatt aaaaccccaa aaagattaaa aaaacaccac aaaacggatg tttcttcaac
    12721 accacttttg ctcatatgaa cggaaccgac gattaaactg gatggctctg attgattcag
    12781 ggtatgaatg gcggtttttt gctccgtttc cctcaaaatg gacgcaactt tccctctgcg
    12841 gctctcagcc gcaccaccgc atccgggcca gcggctcatg catcaggacc tgctctgcca
    12901 gacggtagcc ccgcttcagc cccgtaaaac gcatctgact cccgcacagc acgcacttca
    12961 gcgggtcaac cttcagtaac ctctgataca tccctctcca ggtgatttgc atcgccgttt
    13021 ttctcactgt ctccgttatg atgtacacca cttcttccag taaccgccgt ttcgccggac
    13081 tcaaaaaacc gtagtacctc accatacgga accccttatc cgccacatgc caggagaacc
    13141 tttccatgaa ctcatctcca ctcatcaaca ggtattcttc ccgttttgtt cggtgactgt
    13201 tgtaacgcag accgatttca tcctgaccgg cataatgctc cagacgactc atcggcaccg
    13261 gtggcttttt caggtaagag ccaaagtaca ccgccacatg gggggcatta tccatcaccc
    13321 gggatacgtt gacattccag ccacggcggt aatgcgtgtc caggaagcga ttccattccc
    13381 gtttactgct tccttctgct gccagcgcat ccggcatcac caggtcaggg tatttccgtg
    13441 acagcaaccg tgttatccgg tagcgccaca tgctcatcac cttacgggcg taaaaatgaa
    13501 gatttttcca ggtgtggccc gacgtcacac caccggcagt tgtcgataaa tggatatgcg
    13561 gatgccactg ctggtcacgc ccccatgtgt ggatcaccgt gaatatcccc ggctccacat
    13621 ctgcctgatg gcagatttcc agtatcacat ccgctgcaat gcggctcatc tctgtcagta
    13681 accaccggtt gtggaacacc agggaccagt actggcaggg aagtgtgaac acaatatgct
    13741 gccacgggca gtcggggacc aggctcagca gatactgtat ccactgtgcg ccagccttca
    13801 ccccgcagtg cgggcaggag cggcttttac accggaagca gacctttttt gtatggcaac
    13861 agtccggtga tgaacagcac cactgtgtat accccatcag tgtggtcccg cacgccatga
    13921 ttttggtcac cgactcaatc accaccggac gtactgccct ttccggctgc ttctccagcc
    13981 agttaagcca gtggtttccc tgctgaaaga tatcggcaaa acggggaagc atcagaaggg
    14041 cggggcgact ccgtccggcc agtgaaccgt gccacactcc gggcagtaca taccgccggc
    14101 gctgataccg gaaagaatgg tcgcaaattc ccgctccgtg cagcgggcga tttccggata
    14161 cccttcgtca tcaacacgta caaaccagaa gaccagcttt ttgtttcccg catccacaaa
    14221 gaacggaata ttcaggtctg cgcagcattc aacggcatcg tcaaaactat caaagcgcag
    14281 aacttctgcg tcttcttcgt caaaaaaatc atcttcgtga agcttcacga catagcgggg
    14341 aagtttgctt ctttgagagg cgggttta
//
LOCUS       DAZPEP010000104        14297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZPEP010000104 DAZPEP010000000
VERSION     DAZPEP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.104"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            19..1236
                     /gene="rtcB"
                     /locus_tag="ACHUT9_002962"
     CDS             19..1236
                     /gene="rtcB"
                     /locus_tag="ACHUT9_002962"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFR1349380.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRDLAY
                     FMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEEINC
                     HHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCSCSH
                     GAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSDLVE
                     VIYTLRQVVCVKG"
     gene            1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHUT9_002963"
     CDS             1240..2256
                     /gene="rtcA"
                     /locus_tag="ACHUT9_002963"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFR1349381.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHUT9_002964"
     CDS             complement(2299..5004)
                     /gene="malT"
                     /locus_tag="ACHUT9_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFR1349382.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5615..8008
                     /gene="malP"
                     /locus_tag="ACHUT9_002965"
     CDS             5615..8008
                     /gene="malP"
                     /locus_tag="ACHUT9_002965"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFR1349383.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLVGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8018..10102
                     /gene="malQ"
                     /locus_tag="ACHUT9_002966"
     CDS             8018..10102
                     /gene="malQ"
                     /locus_tag="ACHUT9_002966"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFR1349384.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHUT9_002967"
     CDS             complement(10147..11463)
                     /gene="gntT"
                     /locus_tag="ACHUT9_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1349385.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHUT9_002968"
     CDS             complement(11824..12399)
                     /gene="nfuA"
                     /locus_tag="ACHUT9_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFR1349386.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHUT9_002969"
     CDS             complement(12458..13141)
                     /gene="gntX"
                     /locus_tag="ACHUT9_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFR1349387.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13179..13949
                     /gene="bioH"
                     /locus_tag="ACHUT9_002970"
     CDS             13179..13949
                     /gene="bioH"
                     /locus_tag="ACHUT9_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFR1349388.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13978..>14297)
                     /locus_tag="ACHUT9_002971"
     CDS             complement(13978..>14297)
                     /locus_tag="ACHUT9_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFR1349389.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3440 a         3565 c         3992 g         3300 t
ORIGIN      
        1 cgaaaagaga aaaacaaaat gaattacgaa ttactgacca ctgaaaatgc cccggtaaaa
       61 atgtggacca aaggcgtgcc ggtagaagcc gatgcgcgtc agcaacttat taatacggcg
      121 aagatgccgt ttattttcaa acatattgcg gtaatgcctg atgtacacct gggtaaaggt
      181 tccaccattg gtagcgtgat cccgaccaaa ggggcgatta ttccggcggc ggtgggcgtg
      241 gatattggct gtggaatgaa cgcgctgcgt accgcgttaa cggcggcaga cctgcctgaa
      301 aacctggcgg agctgcgtca agcgattgaa acggccgtac cacacgggcg tactaccggg
      361 cgttgtaaac gtgataaagg cgcatgggaa aacccgcctt ttaacgtcga tgcgaaatgg
      421 gctgagctgg aggccggtta tcagtggtta acgcaaaaat atccgcgttt ccttaacact
      481 aacaactata aacacctggg aacgggtaac cactttattg aaatctgcct tgatgagtcg
      541 gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc catcgggact
      601 tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt gccgtcgcgt
      661 gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag agccgtggcc
      721 tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt ggtaacggca
      781 ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat ggaagagatc
      841 aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat ctacgtgacg
      901 cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg gtcgatggga
      961 gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc gtgcagccac
     1021 ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt ggaagatcaa
     1081 attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga cgaaatcccg
     1141 atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt ggaagttatc
     1201 tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat gattgcgctg
     1261 gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag cctgtcgatg
     1321 ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa accggggctg
     1381 ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc aacggtggaa
     1441 ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg cggcggcgag
     1501 taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac ggtgctgccc
     1561 gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac cgataacccg
     1621 tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc gaaaatagga
     1681 attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg cggcgtggtg
     1741 gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga gcgcgggaac
     1801 attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt tgctgagcgt
     1861 gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca taacctgccg
     1921 cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa tatcactgaa
     1981 cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc gcagttagtg
     2041 aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct cgccgaccag
     2101 ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca tccttcatgc
     2161 catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt tggtttgata
     2221 gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa ccggatccaa
     2281 tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt tctgcgcgtg
     2341 ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc ggatatgcgt
     2401 cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc tgtaaccgga
     2461 gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgc ggatcagttc
     2521 tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat gggcgaattt
     2581 atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct gttccagttc
     2641 cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt cgccttcgat
     2701 gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca gcaacacgcg
     2761 ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca acaggttacg
     2821 gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt cgagaacaat
     2881 ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc gccattgacc
     2941 ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc agttggcagc
     3001 ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg cgttagagat
     3061 ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca gctggctacg
     3121 ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca ggcactgaag
     3181 ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg acgcttcggc
     3241 ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca gaaactcatg
     3301 catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt cctgtgtttc
     3361 ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga ttaaactcca
     3421 taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct gcattagcgc
     3481 cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg aggttgctac
     3541 aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg ccagccgttc
     3601 cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt taaattctgc
     3661 gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta gcagggtgtt
     3721 aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca ctaactgcgg
     3781 attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca gcgacagttc
     3841 gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca tcagcgcatc
     3901 gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca tccagctttc
     3961 tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc agcgctggcg
     4021 caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat ccatccgctg
     4081 taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt cttcgccagt
     4141 cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca acagaaaatg
     4201 gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg aaagatggct
     4261 ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat tctgccgggc
     4321 ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc aaatccaact
     4381 gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct tcgcttcctg
     4441 atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa cacgcagatt
     4501 ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat tttctggttg
     4561 atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca gatgatagtc
     4621 atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa gctgggcgaa
     4681 gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata tcgcacagtg
     4741 accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct cttgctggtt
     4801 atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc actgggaaat
     4861 gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga agttgttcgc
     4921 gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc gaaccggacg
     4981 acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg aggaaatatt
     5041 atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt ctaatgcaag
     5101 cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa tctttattaa
     5161 caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat gttcatcaga
     5221 ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa aaagatgatc
     5281 ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt gaaactgttt
     5341 cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc aacggccatt
     5401 ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc taatcctgtg
     5461 gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg caactcctcc
     5521 ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac tagcgataac
     5581 gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt ttaacgataa
     5641 gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt ctgcggctga
     5701 aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa tgctgcgtgc
     5761 tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct caatggagtt
     5821 tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc aggatgtaca
     5881 ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag agatcgaccc
     5941 ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact caatggcaac
     6001 tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt tccgccagtc
     6061 ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta actacccgtg
     6121 gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag tgacgaaaga
     6181 cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc ccgttgtcgg
     6241 ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg cgcatccgtt
     6301 tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg gcatcaatgc
     6361 ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta aaaagctgcg
     6421 cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc gtcgccatca
     6481 tctggtgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc tgaacgatac
     6541 ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc accagatgag
     6601 ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc ataccctgat
     6661 gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc cgcgccatat
     6721 gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa cctggccggg
     6781 cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc atatggcgaa
     6841 cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact cggatctggt
     6901 ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc ataacgtcac
     6961 caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg cggctctgtt
     7021 ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca atctggaaaa
     7081 attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg cgaataaagt
     7141 ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac aggcgatttt
     7201 cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc tgctgcatat
     7261 tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac cgcgcgtctt
     7321 cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta tctttgcgat
     7381 caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt tgaaggtggt
     7441 gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg cggatatctc
     7501 cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga aactggcgct
     7561 caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg ccgagaaagt
     7621 cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg caattctggc
     7681 caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg cagtattgaa
     7741 agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc agatgctgca
     7801 cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg cagcctatgt
     7861 tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga ctcgcgcggc
     7921 gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc gcgattatca
     7981 ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac gtctggataa
     8041 tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta aaccgcagtc
     8101 gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta ccgccacgaa
     8161 agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa aaatgccgat
     8221 ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag gaacacagta
     8281 caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc cggaaggtta
     8341 tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga ttgtcgcccc
     8401 gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg gtgcctgcgt
     8461 tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg gcgatctcaa
     8521 agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga acccgattca
     8581 tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt ctcgccgttg
     8641 gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta gcgaagaggc
     8701 tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc gggatgccga
     8761 ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa tggcgtggaa
     8821 aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg ttgcagatca
     8881 aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc aagtgaaaga
     8941 ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg tggattcacc
     9001 agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc tctggttgca
     9061 gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct atgaaatgcc
     9121 gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg aaacctggtg
     9181 tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata tcctcggccc
     9241 gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg cgcgtgccta
     9301 tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat tacgaattga
     9361 ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg cagatcaggg
     9421 cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg aaagtaaacg
     9481 tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga ttgtcggtaa
     9541 gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg accacgagaa
     9601 gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg cgacacatga
     9661 cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca aaaccctggg
     9721 gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac tggcgaagca
     9781 agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg ggcataaggc
     9841 atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca ttgccgacag
     9901 taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg aaccggtgaa
     9961 tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg caacgcttga
    10021 gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca gacggcgcag
    10081 agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat ccggcagtca
    10141 gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca ccgagataat
    10201 ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa agtactcttt
    10261 gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg aacccaccgc
    10321 aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg cgataccacc
    10381 cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg cagcaatcga
    10441 ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa tgtatttgtc
    10501 cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga tcaacagcat
    10561 catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca ttgaacgacc
    10621 acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg ccattaccgg
    10681 gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga tcatttcggc
    10741 aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc tgacgccaaa
    10801 gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg ggattggctt
    10861 atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg tcgggattgc
    10921 cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg tggcaatcgc
    10981 agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag ccgccattgg
    11041 tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca ccagcggcag
    11101 catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac cggttaacac
    11161 caccgcccac tggatgtgct ttttaccaaa tttggcaatc agcgtggtgg cgatacgttg
    11221 tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca tgatcagggc
    11281 aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt tatccagcgg
    11341 cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga agccgttcat
    11401 tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa tgactaatgg
    11461 catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac gacaaactct
    11521 aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca ctaaaactaa
    11581 ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac gggtaacatt
    11641 tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg gtcatattat
    11701 cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc tggcacatgt
    11761 tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa aagaggggat
    11821 aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca cacctttcag
    11881 ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca catcgaccat
    11941 ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt cggtgatttc
    12001 catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg actgcagcat
    12061 atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt tggcgttcgg
    12121 ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct ctgcatcttc
    12181 caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt tcagggcagt
    12241 gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag cgttaggcgt
    12301 gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg ccagcagttt
    12361 ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct aatagttgac
    12421 tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga caaaggcacc
    12481 agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct gcgacggtac
    12541 ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg ggcaattcaa
    12601 gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa tgctgggtcg
    12661 cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac cagcgtgata
    12721 acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc cgctgccata
    12781 acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga gcgtgtaaga
    12841 cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg gaaaatttaa
    12901 gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc agtctttgcc
    12961 agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg gcgggtaatc
    13021 cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc gaacaaatcc
    13081 cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt actgttagca
    13141 tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt tggtggcaga
    13201 ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg aatgccgaag
    13261 tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt gttgatctgc
    13321 ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg gccgaagccg
    13381 tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc gggctggtgg
    13441 caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc gtggcgtcgt
    13501 caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg ctggcgggat
    13561 ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg gcgttacaaa
    13621 ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc gttctggcgt
    13681 taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa acggttgatc
    13741 tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc tatctcgatg
    13801 gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc agcaaatcat
    13861 atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag ttttgtcacc
    13921 tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga cagaaattta
    13981 ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga tgtccgcaag
    14041 agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt gttccccctc
    14101 ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg aacgttcggc
    14161 aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta tgtaattaaa
    14221 cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg ccatttgctc
    14281 taccagcccc atcaggt
//
LOCUS       DAZPEP010000105        14051 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZPEP010000105 DAZPEP010000000
VERSION     DAZPEP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.105"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1846)
                     /locus_tag="ACHUT9_002972"
     CDS             complement(<1..1846)
                     /locus_tag="ACHUT9_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1349390.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIVLKARRTSSTMVVNTVASENPD
                     EAGRYSMDVEHGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVRPEA
                     LRRFEQMVEEVSRNASAVAQNTAAAKKSASEAATHATDAAASARAASTSAGQAASSAQ
                     SASSSAGTASTKAREAAKSAAAESSKSAAATSASAAKTSETNAAASQKSAATSASTAT
                     TKASEAATSARGAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKTSETNAKSS
                     ETAAGQSASAAAGSKTAAALSASAASTSAGQASASATAAGKSAESAASSASTATTKAG
                     KATEQATAAARSASAAKTSETNAKTSADNAASSKAAAASSASSAASSASSASASKDEA
                     TRQASAAKGSATTATTKASEAAGSATAASQSKVAAESAATRAETAAKRAEDIASAVAL
                     EDASTTKKGIVQLSSATNSTSESLAATPKAVKAVMGETNKKAPLNSPALTGTPTTPTA
                     RQGTNNTQIASTAFVMAAIAALVDSSPDALNTLNELAAALGNDPNFATTMTNALAGKQ
                     PKDATLTALAGLTTAAGKFPYFTGNDVASLATLTKVGRDILAKSTVAAV"
     gene            complement(1998..2597)
                     /locus_tag="ACHUT9_002973"
     CDS             complement(1998..2597)
                     /locus_tag="ACHUT9_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1349391.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMVGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVTIDLAYEGSGSGDWRTDAFIVGIGYRI"
     gene            complement(2665..6099)
                     /locus_tag="ACHUT9_002974"
                     /pseudo
     CDS             complement(2665..6099)
                     /locus_tag="ACHUT9_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(6443..7123)
                     /locus_tag="ACHUT9_002975"
     CDS             complement(6443..7123)
                     /locus_tag="ACHUT9_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1349392.1"
                     /translation="MATTNAFSLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
     gene            complement(7021..7764)
                     /locus_tag="ACHUT9_002976"
     CDS             complement(7021..7764)
                     /locus_tag="ACHUT9_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1349393.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAALACM"
     gene            complement(7775..8473)
                     /locus_tag="ACHUT9_002977"
     CDS             complement(7775..8473)
                     /locus_tag="ACHUT9_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1349394.1"
                     /translation="MQDIREESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(8473..8802)
                     /locus_tag="ACHUT9_002978"
     CDS             complement(8473..8802)
                     /locus_tag="ACHUT9_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1349395.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(8799..11372)
                     /locus_tag="ACHUT9_002979"
     CDS             complement(8799..11372)
                     /locus_tag="ACHUT9_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1349396.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLADAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAK
                     KDYESTQKKPSGVKVSAGERQEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWK
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKIEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKNAATQTFDGIAQNMAAMLIGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASASTGTAIQAAAANFHFATG
                     GFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
     gene            complement(11353..11766)
                     /locus_tag="ACHUT9_002980"
     CDS             complement(11353..11766)
                     /locus_tag="ACHUT9_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1349397.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(11793..12224)
                     /locus_tag="ACHUT9_002981"
     CDS             complement(11793..12224)
                     /locus_tag="ACHUT9_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1349398.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(12238..12990)
                     /locus_tag="ACHUT9_002982"
     CDS             complement(12238..12990)
                     /locus_tag="ACHUT9_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1349399.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(12998..13393)
                     /locus_tag="ACHUT9_002983"
     CDS             complement(12998..13393)
                     /locus_tag="ACHUT9_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1349400.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            complement(13390..13965)
                     /locus_tag="ACHUT9_002984"
     CDS             complement(13390..13965)
                     /locus_tag="ACHUT9_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1349401.1"
                     /translation="MAIKGLDQAIDNLNRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETRVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            complement(13980..>14051)
                     /locus_tag="ACHUT9_002985"
     CDS             complement(13980..>14051)
                     /locus_tag="ACHUT9_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1349402.1"
                     /translation="PDDGGSCYLWLNRGQPPAVNRRR"
BASE COUNT         2818 a         4392 c         3500 g         3341 t
ORIGIN      
        1 taacggcagc aacggtcgat ttcgcaagaa tatcccgccc gacttttgtc agggttgcca
       61 ggctggcgac atcattcccc gtaaaatacg gaaacttgcc tgccgcagta gtaagtccgg
      121 ccagcgccgt cagggtggca tctttcggtt gcttacccgc aagcgcgtta gtcatggtgg
      181 tcgcaaaatt cgggtcgttg cccagcgccg cagccagctc gttcagcgtg ttcagtgcat
      241 caggtgacga atctacaagg gcggcaatcg cagccataac gaaagccgtg cttgcgattt
      301 gggtattatt cgttccctgt cgcgcagttg gtgttgttgg cgttccggtc agtgcaggac
      361 tatttaaggg cgctttcttg ttcgtttcac ccattaccgc cttaaccgct tttggcgttg
      421 ccgccagtga ctcggaagtg ctgttggtcg cactgctgag ctgtactatc ccctttttcg
      481 tcgtgctcgc atcctccagc gccacggcgg atgcaatatc ctctgcccgt tttgctgctg
      541 tctcggcgcg cgttgccgcg gattcagcag caactttgct ctgagatgca gccgtcgcac
      601 tgcctgccgc ctctgatgct ttcgttgttg ctgtcgtggc actacctttc gctgctgacg
      661 cttgtctggt cgcctcatct tttgaagcag acgcagatga tgccgatgac gccgctgaac
      721 tggctgacga tgcggctgct gccttagagg aagcagcatt gtctgctgaa gtctttgcat
      781 ttgtttcaga ggtttttgct gcagaagcag acctcgctgc tgcagtggct tgctcagtgg
      841 ctttgccagc cttcgttgtg gctgttgaag cggatgatgc ggcgctttct gccgattttc
      901 cggcggcggt ggcactggct gaggcctgcc cggcacttgt tgacgcggca ctggcagata
      961 atgcagccgc tgtttttgaa cctgccgcag ctgaggcgct ctgtcccgct gctgtttcag
     1021 aagacttagc gttcgtctcg gacgtttttg ccgccttcgc ggaatttcct gccgccgttg
     1081 ccgaggaagc tgcactactg gcacttgatg atgcattcgt ttctgaagat ttcgctgcct
     1141 cttttgaggc cgccgcaccc cgtgccgagg tggcagcttc tgacgccttc gtggtcgctg
     1201 tggatgcaga agtggctgcc gatttttgtg atgctgcggc attcgtttct gacgttttcg
     1261 cggcactggc gctggtagct gccgcgcttt ttgatgactc tgcagcagca ctttttgctg
     1321 cttcacgggc ctttgtcgat gccgttcctg cgctggaaga cgctgactga gccgacgacg
     1381 cggcctgtcc ggctgacgtg ctggcggcac gtgctgaggc tgcagcatcg gttgcatgag
     1441 ttgccgcctc gctggctgat tttttcgcgg ctgccgtatt ctgtgcaacc gcggaggcgt
     1501 tacgtgacac ctcttccacc atctgctcaa agcggcgcag tgcctccggt cggacatcat
     1561 cctccgtcat ggcaccgaga aaatcattca gcgtacctgg tctggaacct tcatagacgg
     1621 taatggtccc ggcatgtgaa ggcggaaaac cttcaaccag cagggtgacg ctgtactgac
     1681 catgctcaac atccatgctg taacgtccgg cttcatccgg attttcagag gccaccgtgt
     1741 tcaccaccat cgtgctgctg gttcgtctgg ccttcagcac aatggtgcag ttctgtactg
     1801 gttttcctgt gccatcttta agcacgccag aaatttttac tgtcatactt ttccaccaat
     1861 aaaaaaagcc cgcagcagtg acgccacggg cttcaggaca gtgtaacttt acgtttcctc
     1921 aaacgcagtt caccccataa ggtggatgaa cctgcgtatc ataacaatat ttacagaaga
     1981 taaatcggcg tctgttgtca gatacggtat ccgataccaa caataaatgc atccgttcgc
     2041 cagtcaccac taccggaacc ttcataagca aggtcaatgg tcacggattc ggtcgggtta
     2101 aactgcacgc cagcccccca cgccagagac gtgttgctgt ggcgaccgtc atcacttccg
     2161 gtcagcacat cgtgcgtttt ccccttgttg tcagttacgc ggagataatc cccggagaaa
     2221 gtcgacacac ggctgtaagc cacacccacc atcgcatacg cgctgaacca ttcattcacg
     2281 cgtacagacg gccccgccat cacgctgaac cagcggttac gcacggaatc ttcatgccag
     2341 cgggtatcgc tgtagtgcgt tttttgctca tcctcagcat tggcataact gaaggacgta
     2401 atcagcccca gcgcgtccgt aaactcataa cggtatttca cgttaatccc gttcagatta
     2461 tcgctgccgg gagcgttcgt acgggcatga agataccccg cgctcagtgt ggactgatgt
     2521 tcagacgccc atgcaggcgc accggatacg gacagacaga tggctgcgga caaaatggct
     2581 gcacaaactt tacgcataat tacctctcgc ttttctgcaa taaaaaaggc gccatttctg
     2641 gcgcccgtat atgggttata aaattcagct gatactgatg cctgcggtgg ctttcttcat
     2701 caccacaacc agcaaatcgc tgatacttgc tgtgggatac cagttattca ccagccatgc
     2761 tgacaccgaa aactccagcg tcatgtggcc gcgaccagct ggcatatcaa taacaccact
     2821 gtaaatcagc gtattatcca gcgcggtacg gttataaatt tcagcaccgt ttttccgtac
     2881 tatcaggcgg catgacgaat aaatatcgtt attctcccgc tcatgtttag cgccgctgaa
     2941 tgccaccgcc ggaataacaa tttgcctgtc aaaaggctga tcgtcataaa tcctgacggt
     3001 aatggtccct gatggccacc gctccggtgc ccgggagtcc cgcggaaaag ccttacccac
     3061 tgttttgact atatcgcctt caatctggtt ggctgacagt ttccccttaa tctgacagtt
     3121 ctcattaatt gtgacattgt tgagcgtccc ggcgttcgca ttcacactgc cactgatatc
     3181 tgcattttta gcggtcagtt ttccgtccgg tgtcagggaa aatgccggag gattaccgcc
     3241 gctggtaatg gtgggagccg tcaggcgttt caggaacacg tcgttcatga atatctgatc
     3301 gccctgacca acaaacatcg gctttgtgtt gccattcgca ggattaatca tcgcaatcct
     3361 gtctgccgcc agcagcacct gactctgcat tcctgctggc gtattctcaa taccggcacc
     3421 gatacccgca atataaaggc gtccgtcctt catctgttgc agcttcacag cccacatgct
     3481 gttcagatta ttatttgtat caacctgaac cttctgtatc tgctggattg ccgcactctg
     3541 gtctttcagt ttcttattga cagtctgcgt gatttcattg ctgacatccg taatggacgt
     3601 cctgatttca gccaggtcag gcgcaagctg accgttatca atctgcgtcc acagctcctg
     3661 agccagatgt gttttcccta tctctccttt gaaaaaatcc aagtaacctt ccgcatcatc
     3721 gctcgcccga ccgacagcct ccacgaatgc cgatttgcca acggtgttca cactgcgaac
     3781 gtaaaaataa taatcatggc ccggtttgat attgatactg gcggctatcc agtacagcgc
     3841 cgtgccaaga tagcgggcgc tggtttcaac ctgcctgata tccgcaatcc gcttttccga
     3901 gaaccagaac tcaaactgta ccgtcgggtc ataaacggca agatgcggcg ttgcggttat
     3961 ctgaaaatag cccggcgtca gctcaatccg cgacggtgct gccggtgcgg caatccggaa
     4021 ggtggtggtg gcaggttcac cctgctggcc atagctgttt atcgcccgca ccgtcagggt
     4081 gtattccccg agcggcaggc cgctgaaacg gtgctccgtg tctgcggtga tggcggtggt
     4141 caccagtctg gcatccgttc ccttaccact ggtcaggcgc agactgaagc gcacaccctt
     4201 caccacccgc ggcgtgtccc atttcgcctg cgccagatac tggccgtcag gtgctgcact
     4261 gccggtggga tgacgctgtt cagggaacct gactgcggct caaagcgggc accgttatcc
     4321 acgatggctt ctttttccag tacgtgctgc accgccgtga tggcaaaggt gccgtccgtg
     4381 ttttcccgga cggagacaca gcggaacagg agacggcgca gtgacggcag ggagagtccc
     4441 cacaccatca ggcagggtac tgacctgtat ccggtccggc gcggggtgtt cggtgatgtc
     4501 cacactcacc ggcttaccgc tgccgttaat caggttcacc gtggcggcac cggtctccgg
     4561 aagtgtcact tcacggtcca gcgtcagagt gcgggtggca gcatcaatgg acaggacacg
     4621 tccgccggtc agggtcccgg catagtcgtt atcacagatt tcaatgatgt caccgggtgt
     4681 gtgccgcagc ccctgagacc cgagcgtgaa atccaccgtc tgcgtttcca gcagttcggt
     4741 ctttatcacc cacagtccgg cacggtgggc ctgaccgcgg ctggtacagc cgaacgcgtc
     4801 catcttcagc aggttgcgtc cgtagcgcag tatggcttcc gggtcttcca ccagttccgt
     4861 ggaggtctgc cagccgttct gcgggtcggt ataattcacc tccaccgccg tgtgccggtc
     4921 cttcagggca ctgaagctgt agcggaaacc cacgccgtta tcatccacca ccacatcgct
     4981 gttggtgtac ggccacacca catccgacgg gcggtcctga acaaacgtca gcgtctggcc
     5041 gttccatacc ggcatacagc gcatcgccga gcagaaatca ctgagcacgt cccatgcctt
     5101 acgctgttgt gccaggtacg cattgaaggt catccgcggc tcggtctccc cgaaactatc
     5161 cgggaccgtc tggtcgcagt actgcccgat ggcatacagc gcccacttgt ccacatccgc
     5221 cgcccccaga cgttttccca tgccgtagcg cgggtgagtc agcatgtccc acagacacca
     5281 ggccggattg ttgctgtatg ccggtttcag gctaccgtcc cagataccac tgtacgtgcg
     5341 tttttccggg tcatagtttg acggcacctg gatgatgcga ccgcggatat ggtagttcac
     5401 cgtcatctgc tggccgccga actgctccgc atccacctgc agccccacaa tggccgtgtt
     5461 cgggtagcac tgtttcacat cgatgatttc ggtgtatgac gaccacagcg tcttattctg
     5521 cagctggtcc gtggtgctgt ccgctgtctc ccggaccatc cggatgttaa aaggacgggg
     5581 aggcagatta tccagaatca ccgacgccag aaactgcgag gtggtcttgc cgttaatggt
     5641 gacatccttt tctgtcaccc agttaccgtt acgctgtaac tgaatcagca ggcggacgga
     5701 tgtcgggttt cggtcaccct ttgacgtggt ctgcaccagt gactgcaccc cgaaggtaac
     5761 ccgcaggcgg tcaatgttcg cggacgtaat ggtgcgcgtc accggttttg ccttcgtcac
     5821 ttccacgccc agtccggttt cagcaccgga ggactcaaag ccttccggtg gtgtctgctc
     5881 ctgctccccg gcgcgtcaga ccgccgtcac accgtgtatc acgggattac cgtccgtgtc
     5941 cgtcagcggg gttttgttca ccaggatact ctgcagtccc ttcaccggac cttctatcag
     6001 tccctcacca atcgcatcaa tcacgctcat catctgcgtg gatttgagat tatccttcgc
     6061 ctcacgaggc gtgtgtgcct taccgccacc ttttcccata cagccttccc ctgaataaat
     6121 taaccgccac ttgccattcc gtacagaagt cggatatcct tcgcccgaaa agcatgaaac
     6181 acatttctgc catgctaaag agaaaccccg gtatcagcag ataccggggt tttctttcat
     6241 gcccaccgat aatcctgttg gttaaaaccg gtaatggcat aaaaattctg aatatcttca
     6301 cattttcaca aactgactgt ggcgcgtata atttctctgc gttaattttt ttgtcgtgat
     6361 ataagaataa ttccttacac ttaatcttcg taactctccc gcagttcctg tccgcgatca
     6421 ctgcgggatt tttttattct ttttacccct gccgcccgat aaccacgacc tttccgcccc
     6481 cgccttcatc acgggtgctg atgtcctggg atatacggcg ggagccaacc agcatttccc
     6541 cgtaaggcac cggcatcggg ttcccctggg caatcatgtt atccagtgag gaaaagtacg
     6601 tgttctgtct gccgttatcc gttgcgcggt aatccggtgt ttttgccttc ggggccagca
     6661 tctgggccac accgcccaga atcatgctgg ctccaagtga aaacagcatc gtggtggcag
     6721 aaaaaccacc ggctgccagg gctgaacccc ataacgccat tgatgccccg gcagtgaaga
     6781 aagagcccac gatggctgcc gcccccagca caatctgcag tccacccttt ccggccccgg
     6841 ccagtcgcgg cacaatgtgg atgaccgttc cctcacccag ctgttcgtga agacgggcgt
     6901 acaccgcctc cggtgccgtg tcataaccgg caatacgtat ctggtaccag ccttcgttca
     6961 tctgacggcg aaagcccggc atctgcatcg acagggcgcg aatggcttcc gctgccgtgt
     7021 tcacatacag gctaaggcgg cggccaaatc gttgtaaatc cccgtgaagg cagatgcgtg
     7081 ccagtggcgg tgacgccaga ctgaatgcgt tcgtcgttgc catttttcgg aatacctctc
     7141 ccgtttactc agttgttcag gcagatggtg aagcagctca ccgttgccgc agtaaatggc
     7201 ggcatggttc ggtaccgaag caccaaagca gcacagcaga atatcgcccg cctgtgcaga
     7261 ggacaggggc acccggtaaa agccggtgac cgccatattg tccaggtaaa ggttctgacc
     7321 gttgcgccac cagtcatcct cgcgatgaaa atccggcatt tcagtccccg ccagatgata
     7381 agcatcccgg aacagcgtgt aacagtccgt caccccgtgc tcaaagcgcc gtcctgtcag
     7441 atgtggcaca cagcggaatt tgtgaatgtc accccggcag accaaccacc agggcagtgc
     7501 gctttttatc tgcaaccgcc ggtcagcctc gctcagccag ggcagcccac cgggatgact
     7561 gtggaccagt gccacaatct ccccctgcat ctctgcccgc agccagtctt ccggtgcgat
     7621 acgaaaatac gcctccggct ctgcggaaat attcacacaa gggatatacc gctccccctc
     7681 cggcgtgctt atcacgaagc cgcacgactc cgcaggcgca caccgccggg catgtgccag
     7741 aatcgctgat tcagtctgtg tcataaaccg ggatttactg cgaaagttta ttaatggaaa
     7801 ggaaaccgcc aaaattgccg acattcctgc gcagttcaca cccgcgcatg cacttgctgc
     7861 atctgtcctt acggatatcc gtggtggggt tgtcgaactc atccgccacc gcaggaccgt
     7921 tatacccgca ttcatctccc cggtaatccc acatacaggt gttcgccagc atgatgcgac
     7981 cgggaaacag cgccccgtcc gtctcggtcg gtgtagccag cacaaacgag gccgtcatgg
     8041 ctgtcagctg cgacatctgc tccaccaccc agcggtcact cagctcctgc tccgggtccg
     8101 cctccggatt gcccgcaacg aaattcaccg catccagaaa acgggcatac acccggcggc
     8161 ggaccaccgt ggcccccacc agactctgca ggtcttccgc catcccggtg accagaccga
     8221 acagattgga caccgtcagc gacggtctgg cactgctgcc ccggccgttc atctcaaagc
     8281 cgctgccgtc aatcgggtat gcctcatact tacgcccctg ccaggtgacc ggctcccctt
     8341 tttcattcag ctcattacag aaaaaatacc gctcaccacc ctgtaccgtc aggtcgattt
     8401 cccagagtac cacccgcggt gactgctctg acttaaccga ctcgttcaga ctttcttcgc
     8461 gaatatcctg catcagttca ccacctgctt aaactccgcg ctgaactcaa cgcgcaacat
     8521 cccgacccgc gcagaccacc cggcacaggt cacctttatc tgccggtatg catagggtgg
     8581 cttccacaaa aatgccttcc agccaccgtg ctctgccagg aacgcttcca gatgccgggc
     8641 ctcctcccgg gtcacggaaa gcgtcaccct gtatgttttc aggtcagcat tcagccctgc
     8701 cgccatacgc tgtgagtacc cgtcaccaaa acgcacttca cgcaccgatg gctgcgagtt
     8761 cacctccata tccggcttca ctttccagcg aaaggttttc atccaccgct ccctgataac
     8821 ataccgccat cacgcaactg cagccggagt tcatcctgtg cccccttgcg ggccatctca
     8881 tacaccgctt tcatcagctg cggccctgcc cgcccgttgg ggccgtcgtt ctgaatcacc
     8941 acgtgattgt tctgattaaa attaatgcct tccgcccgcc gcatctgcgc cggacttccg
     9001 gcaccgccga cataaccacc ttccgcatag ccccgcatca ggcggtacag attgccgaca
     9061 ccaatccggc tggtcgcctc cttcgtgaag acaaactccc cgcgatgaac aatccccgca
     9121 ggttcatatt taccccccgt ccccgtaaat cccccggtcg cgaaatggaa gttcgccgcc
     9181 gcagcctgaa tggccgtccc cgtggaggca gacgcaccac caccgaaagc accgccaatg
     9241 gcgctgccga tactcccgac tatccccacc atcgcctgct tcagaaaaat ctctgtcagc
     9301 atggacagca cagaacgggt gaaaccacgc cagttctgtt cgctgccgat cagcatcgct
     9361 gccatattct gtgcaatacc gtcaaaggtc tgcgtggccg cgtttttaac ctgcgaaaaa
     9421 ctgtccgtcg cactttccgc ccactcgccc cagccggact tcatcccggc catccagctt
     9481 ccacgaagct gctcctccgc agaccaggtg ttcttcagtg cagatgtggc cttcgccagc
     9541 gcagccggat tatcaccgta cacctcacga aggcgctgct cttccgactc ccgctgcgcc
     9601 tgacggtcgg tgagtccgcg ggcttttgcg ctgattgccg cctgcttcgc gctctgctgc
     9661 tgttcaaacc gcgccgcctg ctgtgccagc tcattcagcc gtttctggtg ttcaatcttg
     9721 tcgcccagct cagccagctg gcgtttgtac tccagcgttt ctttctcatg ggccagcagg
     9781 gatttttcct gctcagataa ctgccgtttc gtggcggcct ctttcaggac cacatactga
     9841 ttttccgctt tccataaatc acggcgctgc tggctgattt tttcattcgc accgctgtgc
     9901 ttctccagcg tcctgagctc agtttcaagc gccagcaggg ctgcatgcgc ctggtcttcc
     9961 tggcgctcac cggcagacac cttcacaccg gacggctttt tctgcgtcga ctcataatcc
    10021 ttttttgccg acgccatcag cgtgttgtaa tccgcctgca ggattttccc gtctttcagg
    10081 gccttattca gctcttcctg ccgggcggta tatttctcca gtggcgtcag caggcgctca
    10141 tacgccttct gcgcctctcc ggtatacttc agctgtgacg actcacgctc agccctgtcc
    10201 cttgccgcca gttcaccggc tttttccata tccgactgca gcgttgccgc tgccagaccc
    10261 agacgggcat tttcccggtc atcccatgcg ccctgaaggt tggcccggaa agaggaggtt
    10321 ttaccgcggc gctggctccg actctggtac cactgccatt ttttatccgc ctcatcaaat
    10381 gccttctgcg cactggcgag catatccgct gaggattccg gacgaccgat atccagaatg
    10441 gcatcccaca tcgatttgaa tgccttccct gttttatccg cccaggtctc cagtgttccc
    10501 atgttttctt tcaggcgacg ggtctgctca tcaaagcctt tcgtggcgat atcgttcgcc
    10561 gcctgcaatg ccccggcctc gtctccggaa cgctgcagct gtgcaacata cgcaatctgc
    10621 tctgccgtca cgttacggaa ctggcgcgcc atcgccatca gtcccgacgt cgggtcagtg
    10681 gtcagcttcc cgaaggcttc agcgacttta tccacctcca caccggatgc agacgcaaaa
    10741 cgcgcgacac tctggttgat ggcatcaaac tgttcaccac cacgcacacc ggcattcacc
    10801 agggctgcca gtgactctct cgcctggtta aacgtcagcc ctgctgcctg cccggctctt
    10861 gagagagtca gcatacgatc ggcagtcagt ccggactgat taccggaaag aaccagggtt
    10921 ttattaaacg ctgaaagcgt ggaatctccc tggtaccagg cgtacaccag tgcacctgtc
    10981 gccaccgcca gcgaggtgac cccgaccatc ggcagggtga tcgcatcggc aagccccctg
    11041 aacatgggga tcatcccgcc gaaggagtcc ttcacctgac cgccctgttg cagcaggatc
    11101 agccagggat tctgaccacc ggcaagctgc gtggcgatat ccgtaaactg tgcgggcagg
    11161 gttcgcatgg ccgctttata ctgcccgacg gaaatcccgg ctttttgtgc agccagcgcc
    11221 tggcggctca ggccctgttc aacagcactg gcggtttttc tggcgtcggt atccagacct
    11281 gaaaaatgac gccttacccg gctcatctgc tcatcgaaac ggacagcatc cagactcagg
    11341 tcaataacaa gatcaccaac cggctgggac atatctcaca cctcccggaa tccccgctga
    11401 agccatcatt aatgcggcat catccaccat gacatccgcc acatccgcag acgataaaat
    11461 atcgcgccct ccgtccccac cgaaccggac gcctccggca agtcctgccg ctttctgcat
    11521 cagcattttg tcctcatccg gcctctccac ctgctcttcc tcatgccggg ggacaagcag
    11581 actgaaatca gagggatgca tatccggatc gcaaaaaaac aggctgagta cagcgtacgt
    11641 cagcccggaa aaatgcatat ccagctgggt atcctgaaaa taatgcgtgc ggtaaaaacg
    11701 gcgccagtcg gcatattcgg tggatgtcat cccggcaagc atggcgcgcc agtcgggtct
    11761 ccccatctca cgcgccagtc tgagggcaaa gttcagctcg ccgtcgaaga ctttcccgca
    11821 gaaaaatcat catcagtcag cgtgttattt ttcgccactt cagtaatatc agtatccgga
    11881 cgaacagctt cgatcatccc ggacaggcac aacaccacgt cttccgcccg ggcaatggca
    11941 tcggcaggcc aggtggtgag cacttcctgc tctatcttca tcacggcctc attcattgac
    12001 ggtgactgcg ttttctgtgg atggttatgc cacagggaca tcgccaccag aaacgcgccg
    12061 gttctgacga gatcttccac gcttacctgc aggttgccgc tggattctgc ctgttctgca
    12121 cgccgtttca ggagggcaag atgctcgata cgctgcagcg cagacaattc ggaaagcgtg
    12181 acagacacac cgttatattc aaattgttct gttttcagaa acatgtatta cctccgttta
    12241 ccctgcagcg cccgcttcag taacggtgac ttcagccact gcggcgaact gaccatttcc
    12301 gctcaccaca gggatctgca ccttacctgt cgccacgccg tttaccgtaa ttgtcatatc
    12361 tttcacacta atggtggctt tcgacggatc ggcggaaacc gctctgaacg tcttgtcggt
    12421 tgcactttcc ggctcaaaag aaaccgtcag ggtggttgtt ttcccttttg ccaccgtacc
    12481 ggatgtcggc gtcaccttaa tcgcactgac cggcgtaatt ttgctgcgtt cttccgctac
    12541 agaaggttta cccacgttag tgactttcac cgtgcgggtg atcacttctt tcgccgtcac
    12601 ggccttaccg atactgctga cccagccacg aaacacatcc accgtgccat tcggaaaacg
    12661 gattttatag gcccggacat cgccgctttc aaaccagcct ataagccctt tctggccttc
    12721 ctctcccggt ttccaggcca gcgtaaaact ggtatcacct gcagatttct gcccctgccc
    12781 ggtcgcgctc cagtccgcgt cttcatcatc caggtagtta tcatcatagg attcagccgt
    12841 catctcgccc ggcgtcagat ccttcacctt agccagtcgc tgccagtcat cgtctgacaa
    12901 cgggtttgca taagcatcag ccttgccgtt gtaaacccac agagtggtac cggcaccttt
    12961 taccggctcc aggggatttg gtgttgccat atcgtcctca catctcgtat gtaatggaat
    13021 aagtcagatc cgcagagctc cataacgcca tatcgtcatc acgacgatac tcatagccct
    13081 gcgtaaccat cgtggtaatc agtcctgcca gtgcagggat cgcagtcatc gccggataaa
    13141 tccggctttc catccactga tcgagctccg aatcaggtac ctgtgccggt aaaaacacct
    13201 caatatgcag tgtggcccgc caggtatctg catccagctc ttcaccggta tactctgcat
    13261 ccgtcagata aaccgcgatc gcaggaaaat cctcttcgtc aaaaacaacg gggcgaccat
    13321 caaacagcgt cgccccgtgt tcatgctgct cgagtgcatc cagcactgcg gcacgaatgt
    13381 cagtgtgttt catcgtttta tcgcaatcct cagttgttgt ttcagcgcgt atgccagttc
    13441 tttaggcagg cgttcacgcc ggatacggtc aacattctca tcaaatgcct gtttcagtgg
    13501 ggccgccatc gggattttca ccacctgaat gggaaggcga ttacgctttt tccttccctt
    13561 gtcgtcattg ccctcctcat atctggcctg gggaagacgt tgcataacat gccagcgccc
    13621 attatttaat cgctggataa atgcccgctg ataacgatgc tgaccggctt tgagtatgct
    13681 gttcggacga cgccccagca ttctgatccc cagcttaatc acagggagat caccgcggtt
    13741 aacgataatt ctggcattcg gatttctgac cgtcgcccgt ttcagtctgg accgttcctt
    13801 taccagtttc cgtctcaccc tggtttcccg ggcaacctgt gacgaagact gattaatcgc
    13861 cgttgtggcc acgcggttaa tggtcattgc agaagcagcc ggaatggcgt ttttacgaac
    13921 ccggttcaga ttgtcaatcg cctgatcaag cccttttatc gccataattc accctgcgtt
    13981 tatcgtcgcc ggttaactgc gggtggttgc ccacggttga gccagagata acagctgccc
    14041 ccgtcatccg g
//
LOCUS       DAZPEP010000106        14003 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZPEP010000106 DAZPEP010000000
VERSION     DAZPEP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.106"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..260)
                     /locus_tag="ACHUT9_002986"
     CDS             complement(<1..260)
                     /locus_tag="ACHUT9_002986"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349403.1"
                     /translation="MDKKGIFLIIALLIFILLMLLLFAKLNFNVNTALAILSLILTVL
                     SGTDLVKKYTTVKSTNKSPIDMWMVFGIAISAVSMYGLYIDP"
     gene            295..1020
                     /locus_tag="ACHUT9_002987"
     CDS             295..1020
                     /locus_tag="ACHUT9_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFR1349404.1"
                     /translation="MARISKKESANHNKVMELIHSDKLLTQEDREFIFTNYRGDGIGA
                     TGAFFTPEILAWDFVLDADCTGDCIELCAGIGRLSYYQYLRNKPTHITCVELNPEYVM
                     IGKRVLPEAEWITGNALQYSPDRFYDVAYGNPPFGKISTSKAYTGRYKGSEFEYKVIE
                     HASTFASYGAWIVPQGSAGFVYSGVHCYERRESCKYQKFVKDTGYELHPGVGIDTSIY
                     RDEWNGTKVICESVLVDDDGTNL"
     gene            1513..2259
                     /gene="mipA"
                     /locus_tag="ACHUT9_002988"
     CDS             1513..2259
                     /gene="mipA"
                     /locus_tag="ACHUT9_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFR1349405.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTILAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2349..3203
                     /gene="yeaE"
                     /locus_tag="ACHUT9_002989"
     CDS             2349..3203
                     /gene="yeaE"
                     /locus_tag="ACHUT9_002989"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFR1349406.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(3254..4138)
                     /locus_tag="ACHUT9_002990"
     CDS             complement(3254..4138)
                     /locus_tag="ACHUT9_002990"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFR1349407.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4222..5217)
                     /gene="gapA"
                     /locus_tag="ACHUT9_002991"
     CDS             complement(4222..5217)
                     /gene="gapA"
                     /locus_tag="ACHUT9_002991"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1349408.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5559..5972
                     /gene="msrB"
                     /locus_tag="ACHUT9_002992"
     CDS             5559..5972
                     /gene="msrB"
                     /locus_tag="ACHUT9_002992"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFR1349409.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6014..6286
                     /locus_tag="ACHUT9_002993"
     CDS             6014..6286
                     /locus_tag="ACHUT9_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFR1349410.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(6447..7088)
                     /gene="pncA"
                     /locus_tag="ACHUT9_002994"
     CDS             complement(6447..7088)
                     /gene="pncA"
                     /locus_tag="ACHUT9_002994"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFR1349411.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7099..8115)
                     /gene="ansA"
                     /locus_tag="ACHUT9_002995"
     CDS             complement(7099..8115)
                     /gene="ansA"
                     /locus_tag="ACHUT9_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFR1349412.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(8282..10138)
                     /gene="sppA"
                     /locus_tag="ACHUT9_002996"
     CDS             complement(8282..10138)
                     /gene="sppA"
                     /locus_tag="ACHUT9_002996"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFR1349413.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            10299..10850
                     /locus_tag="ACHUT9_002997"
     CDS             10299..10850
                     /locus_tag="ACHUT9_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFR1349414.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            10967..12010
                     /gene="selD"
                     /locus_tag="ACHUT9_002998"
     CDS             10967..12010
                     /gene="selD"
                     /locus_tag="ACHUT9_002998"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFR1349415.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            12015..13976
                     /gene="topB"
                     /locus_tag="ACHUT9_002999"
     CDS             12015..13976
                     /gene="topB"
                     /locus_tag="ACHUT9_002999"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFR1349416.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         3594 a         3373 c         3639 g         3397 t
ORIGIN      
        1 tgggggtcta tatataatcc atacatagaa acagcggaga tagcaatacc gaaaaccatc
       61 cacatatcta tagggctttt atttgtcgat ttgaccgtag tgtacttttt gactaagtcc
      121 gtacctgata gcactgttaa aattaatgat aatatagcga gggcagtatt tacattaaaa
      181 tttagcttag cgaataaaag caacattagt aaaataaaaa taagtaacgc tatgattaaa
      241 aatattcctt tcttgtccat aaataccccg tcgtattttt aaaaaaggta tcttatggca
      301 aggataagta aaaaggaatc tgctaatcac aataaagtga tggaattaat acattcagat
      361 aagctcctaa cacaagaaga tagggaattc atattcacca attatagggg ggatgggata
      421 ggggctacag gtgccttctt cacacctgaa attttagcat gggattttgt acttgatgct
      481 gactgtactg gtgactgtat tgaactatgt gctggtatcg gtcgcctcag ctactaccag
      541 tacttaagga ataaaccaac gcatattaca tgcgtggaac tcaatcctga gtacgtaatg
      601 atcgggaaga gggtactacc agaggcagaa tggatcactg gtaacgcact acagtactca
      661 ccagaccgtt tctatgatgt tgcctatggt aatccaccct ttgggaagat cagtacctca
      721 aaagcgtata caggccgtta taagggtagt gaatttgaat acaaggtgat tgaacatgca
      781 agtacctttg cgtcctatgg tgcatggata gtaccgcaag gttccgcagg ctttgtctac
      841 agtggggtac attgttatga gcgtagagaa tcatgtaagt accagaagtt tgtgaaggat
      901 actggctatg agctacatcc aggtgtaggc atcgatacaa gtatctacag ggatgaatgg
      961 aatggtacta aggtgatatg cgaatcggtg ttagtagatg atgacggtac aaatctgtaa
     1021 ctaaattagt taagaaatca taaagatagt agtacttgca aatgttccca ttgatgaagt
     1081 tatgcgtcct ttcgtttttt agcacaggtt aagagccgtt catatgaata gtagaggctc
     1141 ccggaagagg taaactgaac gacgtctctt gctaaaacac agcaactctc atgccatttt
     1201 atcgacgatg agaacaacgt gcccggtgat acaccttcaa aattaagcgt agatggcaaa
     1261 tggaaaactt gcctgcacgg ctttcttaat ttcaatggca tatcaacaac tcatccataa
     1321 attttgcata attaatgtaa agacaaggct cgccagtaac gctaaattca tttggctgta
     1381 agcgcggtgt catccgcgtc aggaaaatta aacagttact ttaaaaaatg aaaacgtaaa
     1441 aaggttgggt ttcgatgtat tgacgggtaa actttgtcgc ccgctaaaca tttgtttaag
     1501 gaatgattaa ttgtgaccaa actcaaactt ctggcacttg gagtgcttat cgcaacgtct
     1561 gcaggcgtag cgcacgctga aggtaaattt tccctgggcg caggcgtcgg tgtcgttgaa
     1621 cacccatata aagattacga taccgatgtt tacccagtac cggtaatcaa ctatgaaggc
     1681 gataacttct ggttccgtgg cttaggtggt ggttactacc tgtggaatga cgcaacggac
     1741 aaactttcaa ttaccgctta ttggtcgccg ctttacttca aagcgaaaga cagtggcgat
     1801 caccaaatgc gtcacctgga tgaccgtaag agcaccatga tggctggtct gtcttatgct
     1861 cactttaccc agtacggtta cctgcgtacc atcctggctg gcgataccct ggataacagc
     1921 aacggcattg tctgggatat ggcctggttg tatcgttaca ccaacggcgg cctgacagtg
     1981 accccgggta tcggtgtgca gtggaacagc gaaaaccaga acgaatacta ttatggcgta
     2041 tcgcgcaaag agtccgctcg cagcggtctg cgtggctata acccgaacga cagctggagc
     2101 ccttacctgg agctgagcgc cagctacaac ttcctcggcg actggagtgt ttacggtacc
     2161 gcgcgctata cccgtctgtc tgatgaagtt actgacagcc cgatggtgga taaatcctgg
     2221 actggcctga tttctaccgg gatcacctac aaattctgat aatgcatctt tccagggcat
     2281 tttgtgcata aaaacagggc gtcacgccct gttttgcatt acggtggtgc aatacctggg
     2341 aggaaacaat gcaacaaaaa atgattcaat ttagtggcga tgtctcactg ccagccatag
     2401 ggcagggaac atggtatatg ggcgaagatg ccagtcagcg caaaacagaa gttgctgcac
     2461 tacgcgcggg cattgagctc ggtttaaccc tcattgatac cgccgaaatg tatgccgatg
     2521 gcggtgccga aaaggtggtt ggggaagcat taaccggtct gcgtgataac gtctttcttg
     2581 tctctaaagt ctatccgtgg aatgctggcg ggcaaaaagc gataaatgca tgcgaagcca
     2641 gtttacgccg tctcaatact gattatctcg atctctactt attacacagg tctggcagtt
     2701 tcgcttttga agagactgtt gcagcgatgg aaaaattgat cgcccaggga aaaatccgcc
     2761 gctggggcgt ttctaacctt gattatgctg atatgcagga actctggcag ctgccggggg
     2821 gaaatcagtg tgccactaat caggtgcttt accatctcgg ttcacgaggg attgagtacg
     2881 atctactccc ctggtgccag caacagcaga tgccggtgat ggcttacagt ccattagccc
     2941 aggccgggcg gttgcgcaat ggactgttaa aaaacgcggt agtcaacgaa attgcacatg
     3001 ctcacaatat cagctcggca caagtattgt tggcgtgggt aatcagccat cagggtgtga
     3061 tggcgattcc aaaagcggcc acgattgccc atgttcaaca gaatgcggct gtgcttgagg
     3121 tcgaactttc gtcagcggaa ttaaccatgc tggataaagc gtatccggca ccaaaaggaa
     3181 aaactgcgct ggatatggtg tgatgttgat acgtgccgga tgcatcgcac ccggcacaca
     3241 acattaacgc aaattaacgt ttcgcaacgc gaatggattg cgccagatgt gcaggtttct
     3301 ctttggtgac tttttgcgtt tctgaagcgt aagccgtttc tacacaaaca aatgttttgt
     3361 agccatcatc cggcatatcg cccatgctaa ttgaaagcgc cggtcccggg ttccagccta
     3421 caacgttcag atgatgctgg tggcctacgg cgataatacg attcagcgct tcatcattaa
     3481 tcacgctgca atcttgcgga ttcagataca cgcgatcggt acggtcaggg aaggtctgaa
     3541 taccgtcggt cagtacatct tctttcgcgt cattcacttt atcaatgaag cgatcgccca
     3601 gcccactgac gcttacctta gcgatatcac ccacgttaaa gtaggtatgc agggcagagg
     3661 tggtttcaaa ttcgccatgt gattcaagat cgatttcaca ggttttaccc acgcggaaat
     3721 gcgctaacag cgtaaagtcg tgcggacaga attttttcgt ctcttcgctt tgcgtcaatt
     3781 caaaagtcag cgctacgcca tcagcatctt cattatgtga tttcagcgtc cacggcaggt
     3841 tgcgggcaaa accgtgcgca ggcagacctt gttgtgccgc cggaccaaac cacggccagc
     3901 aaaccggtac gccaccgcga atagcgacgc catttttgaa cggtgtgttg ttgctcaacc
     3961 acagaacttc ttcttcaccc gcaggtttcc acgagagaag gtgtgcgccc tgtaatgcaa
     4021 aagaggcttt tacttgggga tgatcgacca caatgaggtc cagttcatcc agtttacgac
     4081 gggagaggac aggggagatt tgttcgatga ccggaagggc aaaaattttc ttaatcatga
     4141 cgcagtcctt taacttcatt ttatcaggta aaaaaaagag cgaccgaagt cgctcttttt
     4201 agatcacagt ttcatctcaa cttatttgga gatgtgagcg atcaggtcca gaactttgtt
     4261 ggagtaaccg gtttcgttgt cgtaccagga taccagtttc acgaagttgt cgttcagagc
     4321 gataccagct ttagcatcga acacggaagt gcaaacttcg ccgttgaaat cggtagatac
     4381 tacgtcatct tcggtgtagc ccagaacgcc tttcatttcg ccttcagcag cggctttaac
     4441 ggcagctttg atctgctcgt aagttgcagc tttttccaga cgaacggtca ggtcaactac
     4501 ggatacgttc ggggtcggaa cgcggaacgc cataccagtc agtttgccat tcagttctgg
     4561 cagtacttta cctacagctt tagcagcacc ggtagaggac gggatgatgt tctgggaagc
     4621 gccgcggccg ccgcgccagt ctttgtgaga cgggccatca acggttttct gagtagcggt
     4681 agtagcgtga acggtggtca tcagaccttc gatgatgccg aagttatcgt tgataacttt
     4741 agccagcgga gccaggcagt tggtggtgca ggaagcgttg gaaacgatgt cctggccagc
     4801 atatttgtcg aagttagcgc ctttaacgaa catcggagtg ttgtctttag acggaccagt
     4861 cataaccact ttcttcgcac cagcggtgat gtgtttacga gcagtttcgt cagtcaggaa
     4921 cagaccagtt gcttcagcga caacgtcaac accaacttcg tcccatttca ggttagccgg
     4981 atcacgttca gcggtaacac ggattttttt accgttaacg atcagatgac cgtctttcac
     5041 ttcaacggta ccgtcgaaac ggccgtgagt ggagtcatat ttcagcatgt atgccatgta
     5101 atcagcgtct aacaggtcgt tgattgcaac gatctcgatg tcagaacgtt tctgagcagc
     5161 acggaaaaca atgcgaccga tacggccaaa accgttgata cctactttga tagtcatata
     5221 ttccaccagc tatttgttag tgaataaaag gttgcctgta aaattacaaa aaccttacgc
     5281 agcgtcaagc ggaatcgtgt caatcattgc gacaaatcaa tcctgtgcct aagcattacg
     5341 cgactgactc gcctcactct tcctttgggc ttgaaaccac atggggtcgg cgccccgaat
     5401 tttaaagggc aattacgata aaaatgtgat tttcatcacg atttaggtgc agccatttcg
     5461 tcgagatcaa gtttaggaca aaattttgcc agttggtgtt aatgttttgt tagaatcagt
     5521 cacgtaatgt gagcacgatt aaagtgagat gtgagcaaat ggctaataaa ccttcggcag
     5581 aagaactgaa aaaaaatttg tccgagatgc agttttacgt gacgcagaat catgggacag
     5641 agccgccatt tacgggtcgt ttactgcata acaagcgtga cggcgtatat cactgtttga
     5701 tctgcgatgc cccgctgttt cattcccaaa ccaagtatga ttccggctgt ggttggccca
     5761 gtttctacga accggtgagt gaagaatcca ttcgttatat caaagacttg tcacatggaa
     5821 tgcagcgcat agaaattcgt tgcggtaact gtgatgccca tctggggcat gtcttccctg
     5881 acgggccgca gccaacgggc gaacgttatt gtgttaactc tgcttcttta cgcttcactg
     5941 atggcgaaaa cggcgaagaa atcaacggtt gaaaaaacga ttcagcaact tattccacag
     6001 gagcggaaaa attatgaatc ttgatgacat tatcaacagc atgacgcctg aggtatacca
     6061 gcgtttgtcg accgccgttg aactggggaa atggcccgat ggcgttgcgt tgaccgagga
     6121 acaaaaagcg aactgcctgc aactggtgat gctgtggcaa gctcgccata ataccgaagc
     6181 acagcatatg acaattgaca caaatggtca gatggtcatg aagagcaaac agcagttaaa
     6241 agaagatttt ggtatctcag caaagcctat tgctatgttt aagtgaatga gttatctagt
     6301 atttaagcct gttattaagg gagggcaaat atacttgtta ggacgtcgtg ttttagctgc
     6361 aagtaggtta aatgcaacac tgacaagtat tatctggcct acaatgtcat tgaggaaatg
     6421 taggccagag atgaattcaa ctaatattac ccctgtgtct cttcccagtc tgccagcgta
     6481 tatagcgttg ccccagctgc tgacatctcc ataaacgcgt gcgcactgtc ctggggctgg
     6541 atattcacgc cacgacaacc atcggtaatc acatttacct tataacctaa ctgtaacgcg
     6601 tccagcacgg taaacttcac gcaatagtca gtagccaggc ccataacgat caattcatcg
     6661 atttcatgat cgcgtaacca gtcatcgagc gaggttttct gccgacggcc gttatcaaaa
     6721 aaggcactgt aactgtcaac taaaggattt tcgcctttat ggaacaccgc tgcgatcgct
     6781 ttttggttca gtaacggatg taattgtgcg ccttcactgt tctgcacaca gtgatctggc
     6841 cagaaggttt gtggcaaacc gtcgagttgg cctggcgtat aaggctctac accgtgctga
     6901 ctggcaaaac tgccgtgatt cgccgggtgc cagtcctgac tggcgataac cgcttcaccg
     6961 cgcgactggc accagtcaat caggcggtta gcgacatcca ccgtactgtc accttccggc
     7021 acggcgagcg cgccaccagc acagaaatca ttttgtaaat cgaccagtaa cagggcgcga
     7081 gggggcatta cagtctcctt aatcatccgg cgtcagttcg ccgcgcaggt tttggctcat
     7141 ggccttgcga atggtttcag tatccagttc ctggctcagc aggtaatgca gtttggttag
     7201 cgtggcttct acagtcatat ctgcaccgcc aataacgccg gcatgggcga gggcgttacc
     7261 ggtggcataa ccacccatat tcactttacc ggacatacat tgtgtcaggt tgaccaccac
     7321 aataccgcga tcgctggctt cttgtaattc ctgcaggaag gctttgtttt gtggcgcatt
     7381 acccacgcca taggagcgca gaatcaatgc tttcaccggt tggcgcagaa aattgcgcac
     7441 gacgtcagca gaaatccctg gataaatcgt cactacgcca attggttgtg gcgtgattgg
     7501 atgaacgatt aattcccctt caccgtgcgg ggcgggtggc gtattcaaac gacgaatatg
     7561 gataccagct tccagtaacg gaggaaggtt tggagaggca aacgcatcaa aaccatcggc
     7621 atgggctttg gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga gcgttacttc
     7681 gttgatcgga taattcgccg caacgtacaa cgcattcagc agattaattt gtccgtcaga
     7741 gcgtaactca gccagcggga tttgtgaccc tgtcacaatg accggtttac cgagattctc
     7801 gagcatgaac gacagcgcag aggcagtata cgccatcgtg tcggtgccgt gcaggatgac
     7861 aaaaccatca tagtcgtcat agtgcgcttt aatatcttca gcaatatgct gccagtcttc
     7921 tggcgtcata tcagaagaat ccatcagcgg cgtatattca tgaatggtga aatctggcat
     7981 ctccgggcga tggaattccg gcatcagcgc cagttggcgt tgtagatgac ctgacaccgg
     8041 aatataaccc tgctcggaac gctgcatccc gatggtcccg cccgtgtagg cgacgtaaat
     8101 tgatttcttt tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt atatacgtga
     8161 ggcaggagga gcgcacctga tactatgcgg ataaggaaat atggggcgag acagcgggaa
     8221 gaggattcgt cgacgtaaaa aataatgtgg ccgacgatct gtcggccact cagtacaaga
     8281 cttaacgcac gttggcgcag gtcaggcaaa acgcataacg gttttgtggg tcgttaaacg
     8341 cggccagctt gtcgctttca cttttaacgg tagaggctac cgaggcaagc ggtgcaggta
     8401 acatggcctg gaacgcatct ggcaacattg cccggacaga accagacatg ttgtccatca
     8461 ctttgtcgaa gaaggtcggt tcatcaacgt agtattccag atgccactgt ttcacttttg
     8521 ccagctctgc tgctttggcg accgcatcat cgaaatcccc gagactatcg accagcccgt
     8581 tagcttttgc atcctgaccg gtccagacgt ggccctgggc gattttatca atctgctccg
     8641 gcgtcgaatg acgcgcatca gcaaccagcg tgataaagcg tttatagcca ttctcaatgc
     8701 ttaattgcat catctgctgc gcttccggcg gcagtgccct ggtgatagaa acatccgcca
     8761 gcggtgaagt tgagacacca tcagtatgaa caccaatcga atccagacta ttttctacgg
     8821 tggtgatcac gccgaagata ccgatagaac cggtcagggt gctggggtta gccacaatgt
     8881 aattagctgg cgtggaaatc cagtaaccac cagatgccgc catgccgccc atcgatacaa
     8941 ccacaggctt acccgctgcc cgggctgctg ccagttcagc gcgaatcact tcagacgcgg
     9001 taacgctgcc gcctgggcta ttaacacgca ggacaatcgc tttcactttc gggtcaaggc
     9061 gagcgtcgcg gatttgtgcc gcagtggtat caccgccaac attcccctga gtttcctcgc
     9121 catccataat tgcgccatta gcgaagacga caccgatgct gtcaccggta tctgccggcg
     9181 ttttcaatgc gtaatcgtaa taactgatgg cgcgataatt tttatcagtc ttactccagc
     9241 cgaactcttt ggttagagct ttttcgattt cggcactcga tgccagtgca tcgaccagct
     9301 tgttttccag tgcatatttc gcggtatcgc caccggtttt ggttaaaccc tcaagcaacc
     9361 cttgcgcgcc agggaatacc tgctgagcag ggatctgccg gttagcggca acagtattca
     9421 gatagttttg ccacagctca ccaatccagc ggctgtcagc ttcgcgggct gccggtgaca
     9481 tgtcatcacg aataaacggt tcaacggcag atttatacgt acccacgcgg aacacatggg
     9541 tggaaacttt cagcttatcc agcaacgatt tgtagtacag accgttggta gcaaaaccgt
     9601 gcaaatccac cacgccttgc ggtgacagcc aaattttatt ggcgaaactg gcgagataat
     9661 attgcccctg gctgtagttc tcgccaacgg cataaaccgg tttcccgctg tcacgaaact
     9721 ctttcagggc tttgccgatg tactgcatag acggctggtc gccgcctgcg aagtttttca
     9781 gatccatcac gataccggtg atattgcggt cgtccttcgc ctggcgaatg gtgttgacga
     9841 tatcaaacag tgagttttcc tgcagacgat cggaactggc accaagcagc tggcggctta
     9901 atttactaaa ccgctgagaa ctgtcgggtt tatcgacgat cacaccagaa atgtccagca
     9961 gcagtgcgcc acgactggcc gtttctttcg aatcaccacc actgacctgc atccagatcc
    10021 ccacaccaac cagtacgagg aaaataaaga acaggttaag taccatttca cggacgaaat
    10081 tcagcagacg ccacgtccat ttaaaaaatc cggcaataaa tcgccaaagg gttcgcatgt
    10141 attctcccaa cttaaggtca cacctgtcac aggcgcaata gatgtggtta tcgtaatgac
    10201 ccaaccggca cttgtcagca ggaattgcgg actgtgctgt aacaaaatcc actctcgtgt
    10261 taattttgtg aataaatatc acgacaggag ttaatcaaat ggatgcactc gaactattga
    10321 tcaatcgccg tagcgcctcc cgcctggctg aacccgcgcc aacgggtgaa caactgcaaa
    10381 acatcctgcg tgcgggtatg cgtgcgccgg accataagtc catgcaaccg tggcattttt
    10441 ttgtgattga aggggaaggg cgcgagcgtt tcagcgccgt actggaacag ggggcgattg
    10501 ctgccggtag tgatgacaaa gctatcgaca aagcccgtaa tgcgccgttc cgcgcaccgc
    10561 tcatcatcac ggtggtggcg aaatgcgaag aaaatcataa agtcccgcga tgggaacagg
    10621 aaatgtctgc cggatgcgcg gtcatggcga tgcaaatggc agccgttgcc cagggatttg
    10681 gcggcatctg gcgcagtggc gcattaactg aaagtccggt agtgcgtgaa gcattcggtt
    10741 gccgtgagca ggataaaatt gtcggttttc tctacctcgg cacgccgcag ttgaaagcat
    10801 ctacgtcgat taacgtcccg gacccgacgc cgtttgtaac ttatttctga taattgtcgc
    10861 gaaactgtct ggattgtgag caaaggcgca ggaattcaga cactctcact tatcacttca
    10921 cggaatgagg gttaccatac ccggattgca ttgacaggag atgtccatga gcgagaactc
    10981 gattcgtttg acccaataca gccacggagc tggttgcggc tgtaaaattt ccccaaaagt
    11041 gttggaaacc atcctgcaca gtgagcaggc gaagtttgtt gatccgaatt tgcttgtggg
    11101 taatgagacc cgcgacgatg cggcggtgta cgatctgggc aatggcacca gcgttatcag
    11161 taccaccgac ttctttatgc cgatcgttga taatcctttc gattttggcc gcattgcggc
    11221 gactaacgcc atcagcgata tcttcgcgat ggggggcaaa ccgattatgg cgattgcgat
    11281 cctcggctgg ccgattaaca aactttcccc ggaaattgcc cgcgaagtaa ccgaaggtgg
    11341 acgctatgca tgccgtcagg cgggtattgc gctggctggc ggtcactcca tcgatgcgcc
    11401 ggagccgatt tttggtctgg cggtaacggg gatcgtaccg accgagcggg tgaagaaaaa
    11461 cagtaccgca caagccggat gcaaactgtt cctgacgaaa ccgctgggga tcggcgttct
    11521 taccacggct gagaaaaaat cactgctgaa accagaacat cagggactag cgacggaagt
    11581 gatgtgccgg atgaacatcg caggcgcgtc ctttgccaac atcgaaggcg taaaagcgat
    11641 gaccgacgtt acgggttttg gtctgctggg ccatttgagc gaaatgtgtc agggcgcagg
    11701 tgtgcaggcg cgcgttgact atgacgcgat cccaaaattg ccgggggttg aagagtacat
    11761 taagttgggc gcagtgcctg gcggcaccga acgtaacttt gccagctacg gtcatctgat
    11821 gggtgaaatg ccgcgtgaag tgcgcgattt gctgtgcgat ccgcaaactt ctggcggttt
    11881 gctgctggcg gtcatgccgg aagcagaaaa tgaggtcaaa gctacagccg ccgagtttgg
    11941 cattgaactg acggcaattg gcgaactggt gccagcgcgc ggcggtcgtg ccatggttga
    12001 gattcgttaa ttcaatgcgg ttgtttattg ccgaaaaacc gagtctggcg cgcgccattg
    12061 ctgatgtcct gcccaaaccg caccggaaag gcgatggctt tatcgagtgc ggtaatggtc
    12121 aggtggtgac ctggtgtatc ggtcacctgc ttgagcaggc gcagccagac gcctacgaca
    12181 gccgctatgc gcgctggaat cttgcggatt tgccgattgt cccggaaaag tggcaattac
    12241 agccccgacc ctccgtgacc aaacaactta acgtcatcaa acgcttcctg catgaagcca
    12301 gcgaaatcgt tcacgccggg gacccggatc gtgaagggca actgctggtg gatgaagtgc
    12361 tggactatct gcaactggca ccggaaaagc gccagcaggt gcagcgttgc ttgataaacg
    12421 acctgaaccc gcaggcggtt gagcgggcgc tcgaccgtct tcggtccaac agtgagtttg
    12481 tgccattgtg cgtttctgcg ctggcgcgag cgcgtgccga ctggctgtac ggcatcaata
    12541 tgacccgtgc gtataccatt ctcggtcgca atgccggtta tcagggcgta ctttccgtgg
    12601 gacgcgtgca gacacccgtg ctcgggctgg tggtgcgccg cgatgaagag attgaaaact
    12661 tcgtggcgaa agacttcttt gaagtcaaag cacatatcgt gacacctgcc gatgagcggt
    12721 ttaccgctat ctggcaaccg agcgaagcgt gtgaaccgta ccaggatgaa gaagggcgct
    12781 tgttacatcg tccactggcg gagcatgtgg ttaaccgcat tagtggtcaa ccagcaattg
    12841 tcaccagcta taacgataaa cgggaatcag aatccgcgcc gctgcctttt tcgctttcgg
    12901 cgttgcagat tgaagcggca aaacgctttg gtctgagcgc gcagaacgtg cttgatatct
    12961 gccagaagct gtacgaaacg cacaagctaa tcacttatcc gcgttccgat tgtcgctatt
    13021 tgccagaaga acattttgcc ggacgccacg cggtgatgaa tgccatcagc gttcatgcac
    13081 cagatctgtt gctgcagcca gtggtagatc cagatatacg caaccgctgt tgggatgaca
    13141 aaaaggtcga tgcacaccac gccatcatcc cgaccgcacg gagttcagcc atcaacctga
    13201 cggagaacga agcgaaggtc tataacctga ttgcccgtca gtacttgatg cagttctgcc
    13261 cggatgtggt gttccgcaag tgtgttatcg aactggacat tgccaaaggc aaatttgtcg
    13321 ctaaagcgcg ttttcttgct gaagcaggct ggcgcacgct gttaggcagc aaagagcgcg
    13381 atgaagaaaa cgacggtacg ccattgcctg tggtggcgaa aggcgatgag ttgctgtgtg
    13441 aaaaaggtga agtggtagag cggcaaaccc agccgccgcg ccattttacc gatgcaacac
    13501 tgctttcggc gatgaccggg atcgcgcgct ttgtgcagga caaagatctg aaaaagatcc
    13561 ttcgtgcgac cgatggtctg gggacagaag caacccgtgc cgggattatt gaactgctgt
    13621 tcaagcgtgg ttttctgacc aaaaaagggc gctatatcca ctctaccgac gccggaaaag
    13681 cgttattcca ttcgctgcca gaaatggcga cgcgaccgga catgaccgcg cactgggaat
    13741 cggtgctgac gcaaatcagc gaaaagcagt gtcgctatca ggactttatg cagccgctgg
    13801 tggggacgct atatcagctt attgatcaag ccaaacgtac gccggtgcgg cagtttcgcg
    13861 gcattgtggc tccgggcagt ggtggcagtg ctgataagaa aaaggctgca ccgcgtaaac
    13921 gtagtgcgaa aaaaagtccg ccagcagatg aagtcggaag cggggcgata gcgtaagcga
    13981 gtgaatcttt cgtgctattc gag
//
LOCUS       DAZPEP010000107        13964 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZPEP010000107 DAZPEP010000000
VERSION     DAZPEP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13964
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.107"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUT9_003000"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="ACHUT9_003000"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1349417.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="ACHUT9_003001"
     CDS             2273..2662
                     /locus_tag="ACHUT9_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1349418.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUT9_003002"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="ACHUT9_003002"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFR1349419.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUT9_003003"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="ACHUT9_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFR1349420.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="ACHUT9_003004"
     CDS             complement(4321..4521)
                     /locus_tag="ACHUT9_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFR1349421.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="ACHUT9_003005"
     CDS             4687..5232
                     /locus_tag="ACHUT9_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFR1349422.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUT9_003006"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="ACHUT9_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUT9_003007"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="ACHUT9_003007"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFR1349423.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="ACHUT9_003008"
     CDS             complement(6529..7353)
                     /locus_tag="ACHUT9_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFR1349424.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUT9_003009"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="ACHUT9_003009"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFR1349425.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUT9_003010"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="ACHUT9_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFR1349426.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUT9_003011"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="ACHUT9_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFR1349427.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUT9_003012"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="ACHUT9_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFR1349428.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /locus_tag="ACHUT9_003013"
     CDS             complement(11316..11969)
                     /locus_tag="ACHUT9_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFR1349429.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUT9_003014"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="ACHUT9_003014"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFR1349430.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUT9_003015"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="ACHUT9_003015"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFR1349431.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3390 a         3536 c         3712 g         3326 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagc
//
LOCUS       DAZPEP010000108        13901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZPEP010000108 DAZPEP010000000
VERSION     DAZPEP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.108"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            241..1494
                     /gene="kch"
                     /locus_tag="ACHUT9_003016"
     CDS             241..1494
                     /gene="kch"
                     /locus_tag="ACHUT9_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFR1349432.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1549..1722)
                     /locus_tag="ACHUT9_003017"
     CDS             complement(1549..1722)
                     /locus_tag="ACHUT9_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFR1349433.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1865..3325
                     /gene="cls"
                     /locus_tag="ACHUT9_003018"
     CDS             1865..3325
                     /gene="cls"
                     /locus_tag="ACHUT9_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFR1349434.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3360..3689
                     /locus_tag="ACHUT9_003019"
     CDS             3360..3689
                     /locus_tag="ACHUT9_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFR1349435.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHUT9_003020"
     CDS             complement(3742..4746)
                     /gene="oppF"
                     /locus_tag="ACHUT9_003020"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFR1349436.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHUT9_003021"
     CDS             complement(4743..5756)
                     /gene="oppD"
                     /locus_tag="ACHUT9_003021"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFR1349437.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHUT9_003022"
     CDS             complement(5768..6676)
                     /gene="oppC"
                     /locus_tag="ACHUT9_003022"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFR1349438.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHUT9_003023"
     CDS             complement(6691..7611)
                     /gene="oppB"
                     /locus_tag="ACHUT9_003023"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFR1349439.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHUT9_003024"
     CDS             complement(7697..9328)
                     /gene="oppA"
                     /locus_tag="ACHUT9_003024"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFR1349440.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHUT9_003025"
     CDS             complement(10066..10713)
                     /gene="ychE"
                     /locus_tag="ACHUT9_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFR1349441.1"
                     /translation="MIQTFLDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11191..13866
                     /gene="adhE"
                     /locus_tag="ACHUT9_003026"
                     /gene_synonym="adhC"
     CDS             11191..13866
                     /gene="adhE"
                     /locus_tag="ACHUT9_003026"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFR1349442.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3655 a         3435 c         3232 g         3579 t
ORIGIN      
        1 ttatagccgg tcagtgcatt cactaccaca agcagataat aactttatct gtatttcttt
       61 atatgatgtg agcatattca ggaaaaatga cctttcctga ggtacagttg ctcgctcaaa
      121 tcctttgatt actgcggata aagtcatcta ctattgttta acacattcac gcgctgcatg
      181 tatgttcaga aaatgtgcac gctaagataa ttaaacaaaa ttgtggttac agaggtatca
      241 gtgagtcact gggctacatt caaacaaaca gcaacaaatt tatgggtgac ccttcggcac
      301 gacattctcg cgctggccgt ctttttaaat ggattgctta tttttaaaac aatctatggt
      361 atgtcggtca atttgcttga tattttccat atcaaagcat tttcagagtt ggatctctcc
      421 ttgctggcaa acgccccact atttatgctc ggcgtctttc ttgtcctgaa ctccattggc
      481 ttactgttcc gggcaaagct cgcatgggca atcagtatca ttttgttgtt gatagcgcta
      541 atttacaccc tgcattttta tccctggctg aaatttagta ttggattttg catttttacg
      601 ctggtgtttt tgctgatact gcgcaaagac ttctcccaca gtagcgccgc agccgggaca
      661 atttttgcat ttattagttt cacgacatta ctgttttact caacctacgg tgcgctttat
      721 ttaagcgaag gttttaatcc gcgaatagaa agtttgatga ccgcgttcta tttttcgata
      781 gaaaccatgt caaccgtcgg ctatggcgat attgtccctg tttctgaatc agcacgattg
      841 ttcactattt cggtcattat ttccggcatt accgtttttg ccacatctat gacctcaatt
      901 tttggtccgc ttatccgcgg gggattcaac aaacttgtaa aaggaaacaa tcatacaatg
      961 catcgtaaag atcattttat tgtttgcgga cattcgattc tcgccatcaa tacgatcctg
     1021 caactgaatc aacgcggaca aaacgtaacg gttatcagca acttgcctga agatgatatc
     1081 aagcaacttg agcaacgctt aggcgataac gctgatgtta tccccggtga cagtaatgac
     1141 agttcagtat taaagaaagc gggaatcgat cgttgccggg ccattctggc gctgagtgat
     1201 aacgatgcag ataacgcgtt tgttgtactc tcggcaaaag atatgagcag tgatgtcaaa
     1261 acagttctcg ccgtcagtga tagcaaaaac ctgaataaga ttaagatggt acatccggat
     1321 atcattctct caccgcaact gtttggcagc gaaattctgg cgcgagtttt aaatggtgaa
     1381 gagattaata atgatatgct cgtttcaatg ttgttgaact ccggtcatgg cattttcagc
     1441 gataacgatg aacaagaaac gaaagctgac agtaaagaat cagtgcaaaa atagagttta
     1501 aagacgaaag agtaaactta ttgggcaccg caatggtgcc cagtatattt agatattgta
     1561 gatcgcaaac aataacgagt tacgctgttt gtttagaatg cacttcctga tgctgtggat
     1621 acgcatattt cggcgcgatt gcccctcaag ccaacgcgat ttacgtcggc tagcctgacg
     1681 ctgcatgcgc cagcgcccca cttccgttct actccgcttc atgttaacta ctctatgcaa
     1741 taacagaatg gtcattatac ccccaccccc gacgctgacc agtgcttttc ccgcgttttt
     1801 atttgccaga tgaatccata tgcgtaaact cataacaatg cgctttcaaa aggatttcta
     1861 atctatgaca accgtttata cgttggtgag ttggttggcc attctgggat actggttgct
     1921 cattgcaggc gtaactttac gcattctaat gaaacgacgc gcagttccct ccgcgatggc
     1981 ctggctgttg attatttaca ttctgccgtt agtcggaatt attgcctatc ttgccgttgg
     2041 cgagctccat ttaggcaaac gccgcgctga gcgcgccaga gcgatgtggc cttccaccgc
     2101 aaaatggctt aacgacctta aagcctgtaa gcatatcttc gccgaagaaa atagcagtgt
     2161 cgctgcgcca ttattcaagc tttgcgagcg tcgtcagggg atcgctgggg tcaaagggaa
     2221 tcagctacaa ctgatgaccg agtcagatga tgtgatgcag gcgttaatcc gcgacatcca
     2281 gctcgcgcgc cataatattg agatggtgtt ttatatctgg cagcccggcg gcatggcgga
     2341 ccaggtggct gaatcattaa tggcggctgc acggcgtggt attcattgcc gattgatgct
     2401 cgactccgcc gggagtgtgg cttttttccg cagcccgtgg cccgagctaa tgcgtaatgc
     2461 cggtattgaa gtggtcgaag ccttaaaggt caatctgatg cgtgtgtttt tacgccgtat
     2521 ggacctgcgc caacatcgca agatgatcat gatcgataat tacatcgcgt acaccggcag
     2581 catgaatatg gtcgatcctc gctacttcaa acaagatgcg ggcgtagggc aatggattga
     2641 tctgatggcg cgtatggaag gccccatcgc caccgcgatg gggattattt attcctgcga
     2701 ctgggagatt gaaaccggaa aacgtattct gccgccacca ccagatgtca atattatgcc
     2761 gtttgaacag gccagcggtc acaccattca cacaattgct tctggccccg gctttccgga
     2821 agatctcatt caccaggcat tattgactgc ggcttattcg gcgcgtgaat atttgatcat
     2881 gacaacgccc tactttgtgc caagcgatga tttacttcat gcgatttgca cggcggcgca
     2941 gcgcggggtg gatgtcagta ttatccttcc gcgaaaaaat gactcgatgc tggtcggctg
     3001 ggccagtcgc gcattcttta cggaactgct tgctgctggg gttaaaattt atcagtttga
     3061 aggcgggtta ctgcatacca agagcgtgct ggtcgatggc gaactaagtc tggttggcac
     3121 cgttaacctt gatatgcgta gtctgtggct gaatttcgag attaccctgg caatcgacga
     3181 taaaggtttt ggtgcagacc ttgccgccgt tcaggacgat tatatttcgc gttcacgtct
     3241 gctcgatgcc cgtttatggc taaaacgtcc attatggcaa cgtgtcgccg agcgactgtt
     3301 ttacttcttc agcccgttgc tgtaaaacgt cgccaacaga ggttaaacag gtagtgacta
     3361 tggatatgga tctaaacaat cgcctgactg aagatgaaac gcttgagcag gcctacgata
     3421 tttttctcga actggctgcc gacaatctcg acccagctga tgttctgctg ttcaatcttc
     3481 agtttgaaga gcgcggcggc gcggagttat tcgatccggc ggaagactgg caggaacatg
     3541 ttgatttcga tttgaacccg gacttttttg ccgaagtggt gattggtctg gcggacagtg
     3601 aggatggtga aatcaacgat gttttcgccc gcattctgtt atgccgcgaa aaagatcaca
     3661 aactttgcca tatcatctgg cgggaataaa taatcgcttt cggcgtgaaa aagggctgac
     3721 aactgtcagc ccttattgtg tttaaagcgg atcgactttc aggcaagaaa cggcgtgtct
     3781 gaaactccct tccagcacag gacgtgtttt ggcgcactcc ggaccggcaa tcgggcaaca
     3841 ggtacggaaa acacaaccgg aaggcgggtt gatcggcgac ggtaattccc cttccagtaa
     3901 ctggatggtt ttgttcttct ccagatccgg atcaggtatg gggactgccg acatcaatgc
     3961 cctggtgtaa ggatgtagtg gattgtggta gacctcatca taggtcccca gttctaccgc
     4021 atggccgaga tacatcacca acacacgatc ggaaatgtgt tttaccacgg ccaggtcatg
     4081 agcgataaaa attaatgaca atcccatctc acgttgcagc tgctggagca ggttgaccac
     4141 ctgcgcctga attgacacgt ccagcgccga aatcggctca tcgcagataa tcagcttcgg
     4201 ttcaagaata agagcacgag caatcccgat acgctggcac tgcccaccgg agaactcatg
     4261 cggatagcgg ttaatcaggt taggcaataa cccgactttc agcatcatcg ccttcacgcg
     4321 ctcgcgaact tcctggcgtg acattttcgg atgataagta cgcagtggtt cagcgatgat
     4381 ctcgccgatg gtcatacgcg ggtttagcga tgccaacgga tcctggaaaa tcatctgaat
     4441 atcactgcga acggcacgcc attcatcggg cttcatgccc agcaactctt tacctaacca
     4501 ggcaacatga ccgtcggtcg ctttaaccag gccaatgatg gcgcgagcaa aggtggactt
     4561 accgcatccc gattccccta ccacacctaa tgtttcccct tcatacaggc gaagcgtgac
     4621 accatcgacg gctttgagag ttttcggcgg ttgccagaac cactgtttgc catctttgat
     4681 ttcaaagtgc actttcagat cggcgatttc gaggaggact tttcttcctt cggttacagc
     4741 attcataaca gttcctccac cggtttaaag caagcacgca gacggccagg cgtaaactct
     4801 tccagcggcg gtgcgctact acaaatttcc atcgcatgcg gacaacgtgg ctggaacggg
     4861 caacctttcg gtaatcgcag caggtttggc ggattaccag ggatggtcaa cattgtttca
     4921 ccttccgcat cgagacgcgg caccgcgttg agcaaaccga tagaataagg atgaacgggt
     4981 tgataaaaga catcgcgcgc gttgccatat tccatcgtgc gcccggcgta cattaccagc
     5041 actttgtcgc agatccccgc caccacgcca agatcgtggg taatcataat gatggcggta
     5101 ttaaattccc gcttcagttc atttaatagc gtcatgatct gcgcctgtac ggtgacgtcc
     5161 agcgcagtag tgggttcatc cgcaatcagc agcttaggtc gacatagcaa tgccatcgca
     5221 atcataactc gctgacgcat gccaccagaa aattcgtgcg ggtacatttt catgcgttta
     5281 cgtgcttccg gcatttttac cgcatcgagc atccgcaccg actcttcaaa cgcctcagct
     5341 ttgctcatgt tcttatgcag catcagcact tccatcaact gctcaccgac gcgcatatag
     5401 ggattcaacg aagtcattgg gtcctgaaaa atcattgaga tttgttcagc gcgcagttta
     5461 ttgagttcac gctctggcaa attgaggatt tcacgcccat tgaaggttgc cgatccgcca
     5521 atacgtccgt tggcagccaa caggcccatc aacgcaaatg cagtttgcga tttacccgaa
     5581 ccagactcac ctacaatgcc cagcgtttct ccggcacgta gagaaaaatt caaatcattg
     5641 accgccgtga catcgccgtc cggggtacta aaggtgacac gcaaatcttt cacgttcagc
     5701 agtgcgtcag cctgttgttg tgcgagcggc acagttgcag tttcaattac gctcatggct
     5761 gcactcctta acgatctttc gggtcgagag catcacgcaa gccatcgccg ataaagttga
     5821 aacaaaacag cgtcaccacg aggaatcccg ctgggaacaa caataaccat ggagagactt
     5881 ccatcgagtt cgcgccatca ctcagcaatg caccccagct gcttaacggc tcttgcgtac
     5941 ccaaccccag gaagctaagg aaagattcaa agaggatcat gctgggcacc agaagtgatg
     6001 cgtagaccac caccacaccg agtacattcg gcacaatgtg gcgaataaca atgcccgatg
     6061 tcgatacacc gccaacttgt gccgcctcaa taaactcttt gcgcttcaga ctcagggttt
     6121 gtccacgcac aatacgagcc atatccagcc aggaaaccat gccaatcgcc acgaaaatca
     6181 gcaggatgtt ttgaccgaaa aaggtcacaa gcaaaatgac gaagaacatg aatgggaagg
     6241 agttgaggat ttccagcaga cgcatcatta ccgaatccac tttaccgccc agataaccgg
     6301 aaagcgaacc gtaaagtgtc cccacgacca ctgccaccag tgccgcagca acaccgacca
     6361 tgagtgagat acgcccgcca atcgcaacgc gcacaagcag gtcgcgaccg gatgagtcag
     6421 taccaaagta gtgaccggac tccatatccg gggcgctgga catcatcgcc cagtcagtat
     6481 cgtcataggc aaactgcgaa agcatcggtg ccaggattac aaataacgcg atcagcacca
     6541 gcactatcag actggcaacc gccgcacggt tatgcataaa acgtcgacgt gcgtcctgcc
     6601 acaagctgcg cccttcgacc tccagctttt cactgaaatt ttccagcgtc tcgctgtttt
     6661 tcttacttaa catcatcgcg agctccagta tcagtaacgg attttcgggt cgataaccgc
     6721 atatagcaca tcgacaatgg cattaaacaa aatggttaaa gcaccaacca ggatggtcag
     6781 gcttaacact aaggaatagt cacggttcaa tgcgccatta acgaacagtt gtccaatccc
     6841 cggcaaacca taaatggttt cgataaccat agaaccggta ataatgccga caaatgcggg
     6901 gcccatataa gagagcacgg gtaacagagc aggttttaat gcgtggcgta aaatgatccg
     6961 ccgcataggt aaccctttcg cccgggcagt acgaataaag ttggagtgta atacttcaat
     7021 catagagcct cgggtaatac gcgcaatact ggcgatataa gcgagtgaca acgccaccat
     7081 cggcaatatc atgaatttaa gcgccccacc attccagcca ccgcccggca gccaatgcaa
     7141 aatgatcgca aatatcatga ctaataatgg cgcaaccaca aaactgggga taacaacccc
     7201 ggtcattgcc agccccatca cggtatagtc ccatttggtg ttttgtttta atgcgacaat
     7261 aacgccagca ctaacaccca gtattaccgc aaggaaaaat gcggcggctc ccagtttggc
     7321 agaaacggga aaactggatg caaccaggtc attgaccgaa taatctttat atttaaacga
     7381 tggaccgaaa tcaccgtgcg ccagttgttt caggtagctg aaatactgtg tcatgatcgg
     7441 atcattaaga tgatatttcg cttcgatatt ggccatcact tccggcggta aagtacgttc
     7501 gccggtaaaa gggcttcccg gcgcgaggcg catcataaag aacgaaatag taataagaat
     7561 aaatagcgtc ggaatcgctt ccagacagcg acgtagaata aattttaaca ttgccctacc
     7621 ttctggcctg tgccttctgt aatgcgataa aaatcagaca ccgtggagca ggacactcct
     7681 gccccacgta ttgccattag tgcttcacaa tgtacatatt ccgggtgtag gtattatcca
     7741 gcggatcttt gccggtatag ccaccaaccc acggtttcac cagacgcgca ttcacgtagt
     7801 aataaacagg aacaatggcc gaatccttat ccagctgttg ttctgcttta gtgtacagag
     7861 ctgtgcgctg cgcctcgtca gtcactttca gcgtttccgc cataatgctg tcaaaggccg
     7921 ggctcttata atgcgcggta ttcatcgagc tgttcgacag catggtgttc aggaaggaag
     7981 ttggttcgtt gtagtctgca caccagcctg cacgggccac atcaaaagtg ccctggtgac
     8041 gggtgtcgag gaacgttttc cactcctggt taaccagttt gacgtttaca ccaatgtttt
     8101 tcttccacaa tgaagaggca gcaatcgcca gctttttatg cagatcagag gtgttataca
     8161 gcaggttgat ggtcaacggt ttgtctgcgg tataacccgc ttcagccagc agttttttcg
     8221 cttcttcgtt acgtttttcc tggctccagc caaaccattc tggctgagtc aattttgcgc
     8281 catcagtata cggtggagtg taaccatagg cgggcatgtc gccctgcgct ttcactttat
     8341 taacaatgat atcgcggtcc atacctagtt tcagtgcggt acgcacacgc acatcgttga
     8401 atggcggttt ctggttgtta atttcgtaat agtaagtgca caggtatgga tcaacgtgaa
     8461 cttcgtccgg gatctctttt ttcagcttct ggaacaattc gatcggcatg ttgttatacg
     8521 tcatgtcgat ttcaccacta cggtagcggt tgacatcggt aacttcagaa gcaataggca
     8581 aataggttac ctggttaata acggttttcg cgttgttcca gtaggtcggg ctgcgttcaa
     8641 gaacgattcg ttcgtttacg acccaatctt ttaaggtata ggcaccgttg gtgacgatat
     8701 taccaggctg ggtccatttt tcgccgaatt tctcgatagc ggcttttggc accggtgaag
     8761 ttgatgggtg aacaagtaat ttatagaagt acggaacggg ttcacttaag gtgacttcta
     8821 atgtgtgatc atcaatagct ttcacgccga gatcggtaat cggttttttc ccttcaagaa
     8881 tttcatcaat accggcgata tgcccatatt gcagataact ggcatacgga gaagcagtgt
     8941 tcggatcaac agaacgttgc cagctataca caaagtcttg tgctgtgact ggcgtgctat
     9001 cagaccattt cgcatcttta cgcaaatgga aggtccagac tttcgcgtct ttattatccc
     9061 aggattcagc gacgccaggt gctggatgac cgtcaagatc gctgaccagt aagccttcaa
     9121 acaggtctcg gctgatatta gactccggaa caccttcaat tttgtgcgga tctaatgact
     9181 gaacttctga accattgtta cgtaccagtg tttgtttttc cgccagtgtg acgcctgcgg
     9241 gtacatcagc tgccagcgcg acattccctg ccattagcgc agccagaacg ccagctgcta
     9301 ctaaacttct cttggtgatg ttggtcattg ttttttggac tccctcatta taattactgg
     9361 tattaaccag catgtgtaat cccggattgg gaccctgcac aggctcggag actttctgct
     9421 gtcaggttac tttatcgtta cgtattacta tcaccgactt tatttattct ggtgattcag
     9481 acgatcacct tatgtcgatt cgtactgtgt ctcccctgtc gagacacatt attctttcgc
     9541 gacgctcttt aattgttaat tattctcatt accaataaag aatcgtctgg cggttgcccg
     9601 gaaagtacca aatgcggttc ttactcgcca atacattttg caaaattgtt aagcaattct
     9661 cttttattga ccgtatcaga atctatagct tgcatccatg ccttaacaaa catctttcaa
     9721 ttcaatcaaa tagagattgg ctaagcatgt ctatcttttc tgattacagt tccagtagtg
     9781 aaatgcacaa taatttaaca attgattatt atttagcact ttcctctacc aaaggcagtg
     9841 gaattactaa tatcatttca attattcttc agcaagccca ggattatgat gtggcgaaaa
     9901 taacatgatg gtgaggagaa gcagttaagg aaatccttat aactaactgt aatttgagaa
     9961 gaatattttt acctattcct tttgcttttg ataactgcgc gggaacaaag taaaaataat
    10021 gacgaagtaa acggagcatg gcagctccgt ttcatttgaa aggaattaat taagcaggcc
    10081 ggggaaaatc cccttaatac cagtaacgat aaattcaatc cccaatgcca tcagcaatag
    10141 ccccataata cgcgtaatca cgttgatgcc ggtctggcgt aaaacccgta ccagccacgg
    10201 tgccatgcgg aacaatcccc agcaacataa agcgaacaat gcaatagcca caaagaaacc
    10261 aaacagatag ctaatgctgt gataacgcgt accccagacg atggtagaac tgatcgcccc
    10321 tggccccgcc atcaacggca acgccaatgg caccacacca atgctttcac gtaccgcggt
    10381 ttctgatttt tcttgcttgt tctgtttatc ctcgccaagc ttgccgctga tcatcgacat
    10441 cgctatcgtc accaccagga tgccccctgc gatacggaac gaatcaattg atataccaaa
    10501 aagttgcaga atcgtgtcgc cgagaaaaag cgagatccac aagataatgg ccacagacag
    10561 gttggctgta aggttagttt tgtttcgcgc tgccgctgtc tgataactgg tcatgctgat
    10621 aaagacggga ataatcccta ccgggttgac cagcgcaaat aacccgatga aaaatttgaa
    10681 gtaaacggga aaatccaaaa aggtctgaat cacggttagc tccgaagcaa aagccggata
    10741 atgttagcca taaattaagg ttgaaaagac gcgctgacaa tacgcctttt gacagcattt
    10801 ttcacctcct aactacttaa aattgctatc attcgttatt gttatctagt tgtgcaaaac
    10861 atgctaatgt agccaccaaa tcatactaca atttattaac tgttagctat aatggcgaaa
    10921 agcgatgctg aaaggtgtca gcttagcaaa aatttgattt ggatcacgta atcagtaccc
    10981 agaagtgagt aatcttgctt acgccaccta gaagtgacgc attagagata ataactctaa
    11041 tgtttaaact cttttagtaa atcacagtga gtgtgagcgc gagtaagctt ttgattttca
    11101 taggttaagc aaatcatcac cgcactgact atactctcgt attcgagcag atgatttact
    11161 aaaaaagttt aacattatca ggagagcatt atggctgtta ctaatgtcgc tgaacttaac
    11221 gcactcgtag agcgtgtaaa aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa
    11281 gtagacaaaa tcttccgcgc cgccgctctg gctgctgcag atgctcgaat cccactcgcg
    11341 aaaatggccg ttgccgaatc cggcatgggt atcgtcgaag ataaagtgat caaaaaccac
    11401 tttgcttctg aatatatcta caacgcctat aaagatgaaa aaacctgtgg tgttctgtct
    11461 gaagacgaca cttttggtac catcactatc gctgaaccaa tcggtattat ttgcggtatc
    11521 gttccgacca ctaacccgac ttcaactgct atcttcaaat cgctgatcag tctgaagacc
    11581 cgtaacgcca ttatcttctc cccgcacccg cgtgcaaaag atgccaccaa caaagcggct
    11641 gatatcgttc tgcaggctgc tatcgctgcc ggtgctccga aagatctgat cggctggatc
    11701 gatcaacctt ctgttgaact gtctaacgca ctgatgcacc acccagacat caacctgatc
    11761 ctcgcgactg gtggtccggg catggttaaa gccgcataca gctccggtaa accagctatc
    11821 ggtgtaggcg cgggcaacac tccagttgtt atcgatgaaa ctgctgatat caaacgtgca
    11881 gttgcatctg tactgatgtc caaaaccttc gacaacggcg taatctgtgc ttctgaacag
    11941 tctgttgttg ttgttgactc tgtttatgac gctgtacgtg aacgttttgc aacccacggc
    12001 ggctatctgt tgcagggtaa agagctgaaa gctgttcagg atgttatcct gaaaaacggt
    12061 gcgctgaacg cggctatcgt tggtcagcca gcctataaaa ttgctgaact ggcaggcttc
    12121 tctgtaccag aaaacaccaa gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa
    12181 ccgttcgcac atgaaaaact gtccccgact ctggcaatgt accgcgctaa agatttcgaa
    12241 gacgcggtag aaaaagcaga gaaactggtt gctatgggcg gtatcggtca tacctcttgc
    12301 ctgtacactg accaggataa ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa
    12361 acggctcgta tcctgattaa caccccagcg tctcagggtg gtatcggtga cctgtataac
    12421 ttcaaactcg caccttccct gactctgggt tgtggttcct gggggggtaa ctccatctct
    12481 gaaaacgttg gtccgaaaca cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac
    12541 atgttgtggc acaaacttcc gaaatctatc tacttccgcc gtggctccct gccaatcgcg
    12601 ctggatgaag tgattactga tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg
    12661 ttcaacaatg gttatgctga tcagatcact tccgtactga aagcagcagg cgttgaaact
    12721 gaagtcttct tcgaagtaga agcggacccg accctgagca tcgttcgtaa aggtgcagaa
    12781 ctggcaaact ccttcaaacc agacgtgatt atcgcgctgg gtggtggttc cccgatggac
    12841 gccgcgaaga ttatgtgggt tatgtacgaa catccggaaa ctcacttcga agagctggcg
    12901 ctgcgcttta tggatatccg taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg
    12961 aaaatgatcg ctgtcaccac cacttctggt acaggttctg aagtcactcc gtttgcggtt
    13021 gtaactgacg acgctactgg tcagaaatat ccgctggcag actatgcgct gactccggat
    13081 atggcgattg tcgacgccaa cctggttatg gacatgccga agtccctgtg tgctttcggt
    13141 ggtctggacg cagtaactca cgccatggaa gcttatgttt ctgtactggc atctgagttc
    13201 tctgatggtc aggctctgca ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac
    13261 cacgaagggt ctaaaaatcc ggtagcgcgt gaacgtgttc acagtgcagc aactatcgcg
    13321 ggtatcgcat ttgcgaacgc cttcctgggt gtatgtcact caatggcgca caaactgggt
    13381 tcccagttcc atattccgca cggtctggca aacgccctgc tgatttgtaa cgttattcgc
    13441 tacaatgcga acgacaaccc gaccaagcag actgcattca gccagtatga ccgtccgcag
    13501 gctcgccgtc gttatgctga aattgccgac cacttgggtc tgagcgcacc gggcgaccgt
    13561 actgctgcta agatcgagaa actgctggca tggctggaaa cgctgaaagc tgaactgggt
    13621 attccgaaat ctatccgtga agctggcgtt caggaagcag acttcctggc gaacgtggat
    13681 aaactgtctg aagatgcatt cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg
    13741 atctccgagc tgaaacagat cctgctggat acctactacg gtcgtgatta tgtagaaggt
    13801 gaaactgcag cgaaaaaaga agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc
    13861 gcttaatcag tagcgctgtc tggcaatata aacggcccct t
//
LOCUS       DAZPEP010000109        13853 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZPEP010000109 DAZPEP010000000
VERSION     DAZPEP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.109"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHUT9_003027"
                     /pseudo
     CDS             complement(1..736)
                     /gene="dsbG"
                     /locus_tag="ACHUT9_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
     gene            1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHUT9_003028"
     CDS             1108..1671
                     /gene="ahpC"
                     /locus_tag="ACHUT9_003028"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFR1349443.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHUT9_003029"
     CDS             1842..3407
                     /gene="ahpF"
                     /locus_tag="ACHUT9_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFR1349444.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHUT9_003030"
     CDS             complement(3528..3956)
                     /gene="uspG"
                     /locus_tag="ACHUT9_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFR1349445.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4177..5415
                     /locus_tag="ACHUT9_003031"
     CDS             4177..5415
                     /locus_tag="ACHUT9_003031"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFR1349446.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHUT9_003032"
     CDS             complement(5419..5508)
                     /gene="yldA"
                     /locus_tag="ACHUT9_003032"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFR1349447.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHUT9_003033"
     CDS             complement(5646..6056)
                     /gene="rnk"
                     /locus_tag="ACHUT9_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFR1349448.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHUT9_003034"
     CDS             complement(6286..7092)
                     /gene="rna"
                     /locus_tag="ACHUT9_003034"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFR1349449.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHUT9_003035"
     CDS             complement(7206..8669)
                     /gene="citT"
                     /locus_tag="ACHUT9_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFR1349450.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHUT9_003036"
     CDS             complement(8720..9598)
                     /gene="citG"
                     /locus_tag="ACHUT9_003036"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFR1349451.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNFASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHUT9_003037"
     CDS             complement(9573..10124)
                     /gene="citX"
                     /locus_tag="ACHUT9_003037"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFR1349452.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHUT9_003038"
     CDS             complement(10128..11660)
                     /gene="citF"
                     /locus_tag="ACHUT9_003038"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFR1349453.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHUT9_003039"
     CDS             complement(11671..12579)
                     /gene="citE"
                     /locus_tag="ACHUT9_003039"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFR1349454.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHUT9_003040"
     CDS             complement(12576..12872)
                     /gene="citD"
                     /locus_tag="ACHUT9_003040"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFR1349455.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHUT9_003041"
     CDS             complement(12887..>13853)
                     /gene="citC"
                     /locus_tag="ACHUT9_003041"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFR1349456.1"
                     /translation="VDTTVEVFITVTRDEKLITCGGIAGNIIKCVAISESVRGEGLAL
                     TLATELINLAYERHSTHLFIYTKTEYEALFRQCGFSTLTSVPGVMVLMENSATRLKRY
                     AESLKKFRHPGNKIGCIVMNANPFTNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYED
                     RLDLVLKGTADIPRLTVHRGSEYIISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLA
                     PALGVTHRFVGTEPFCRVTAQYNQDMRYWLETPTISAPPIELVEIERLRYQEMPISAS
                     RVRQLLAKNDLTAIAPLVPAVTLHYLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3401 a         3506 c         3688 g         3258 t
ORIGIN      
        1 aatgatatta agcgttttct gatcgggcaa ccccacggcc tgttgcagcg tattttcctt
       61 actcatgtaa tagatagccg gcgtgacatt tgcccccaga tcgtccatca gtttctcatt
      121 gtcacttaac actttcattt gctctgtact tacgtttgca ggcacgttta gcttaagctt
      181 gccaccagag gcttcatatt gttgccaggt ttttgcggga tctttggagg caagaattgc
      241 cgctgctgtc gccgggcttt ctggcttgat aaccccaacc aacaatgttc ttaattgcac
      301 tttgccagaa tctacccacg ggcgcgcctg ctgccagaac tgtttacaat atgggcagaa
      361 cggatcggcg aagacgtaga caatgaccgg cgcatctttt ttaccgtcga ggatccagtg
      421 ggattgttcc atccgttgcc acatttcgcg tccggctggt gcgtaaattt ctttttcgat
      481 aagtgtgtta ctcaggtttt cacctttctc gttgtacatg taaccagaga tagcgtgctt
      541 accatcggga gtcaggtagg tagatggtga cgcccatatc ctgatacttt ccgagataac
      601 ctttcattcc tccgggggca tcgaacgttt tgatgattgt aatgccctgt ttttcaatcg
      661 cttttactgg ggaaggaagt tcctctgcga aggcgattgc aggaagcaga gccagtaaaa
      721 gtatcttttt taacattaat ttgtcctttt cagtcagtgc aaaagtcgag taaaaggcat
      781 aacctatcac tgtcataggt aagagcttag atcaggtgat tgccctttgt ttatgagggt
      841 gttgtaatcc atgtcgttgt tgcatttgta agggcaacac ctcagcctgc aggcaggcgc
      901 tgaagatacc aaagggtagt tcagattaca cggtcacctg gaaagggggc cattttactt
      961 tttatcgccg ctggcggtgc aaagttcaca aagttgtctt acgaaggttg taaggtaaaa
     1021 cttatcgatt tgataatgga aacgcattag ccgaatcggc aaaaattggt taccttacat
     1081 ctcatcgaaa acacggagga agtatagatg tccttgatta acaccaaaat taaacctttt
     1141 aaaaaccagg cattcaaaaa cggcgaattc atcgaaatca ccgaaaaaga taccgaaggc
     1201 cgctggagcg tcttcttctt ctacccggct gactttactt tcgtatgccc gaccgaactg
     1261 ggtgacgttg ctgaccacta cgaagaactg cagaaactgg gcgtagacgt atacgcagta
     1321 tctaccgata ctcacttcac ccacaaagca tggcacagca gctctgaaac catcgctaaa
     1381 atcaaatatg cgatgatcgg cgacccgact ggcgccctga cccgtaactt cgacaacatg
     1441 cgtgaagatg aaggtctggc tgaccgtgcg accttcgttg ttgacccgca gggtatcatc
     1501 caggcaatcg aagttaccgc tgaaggcatt ggccgtgacg cgtctgatct gctgcgtaaa
     1561 atcaaagcag ctcagtacgt agcttctcac ccaggtgaag tttgcccggc taaatggaaa
     1621 gaaggtgaag caactctggc tccgtctctg gacctggttg gtaaaatcta aatttccttc
     1681 gtctttcgcg ctaccaactc cggcgcgaaa tacttcggaa attcacctaa ttcttcgggt
     1741 gctgcggcac ccgattttct tccccgcacc atgatgcaag ctgcatccag gtagccgtag
     1801 aggccgcttg catgatgatg tttaaagccc aggagataaa catgctcgac acaaatatga
     1861 aaactcaact caaggcttac cttgagaaat tgaccaagcc tgttgagtta attgccacgc
     1921 tggatgacag cgctaaatcg gcagaaatca aggaactgtt ggctgaaatc gcagaactgt
     1981 cagacaaagt cacctttaaa gaagataaca gcttgccggt gcgtaagccg tctttcctga
     2041 tcaccaaccc aggttccaac caggggccac gttttgcagg ctccccgctg ggccacgagt
     2101 tcacctcgct ggtactggcg ttgctgtgga ccggtggtca tccgtcgaaa gaagcgcagt
     2161 ctctgctgga gcagattcgc catattgacg gtgattttga attcgaaacc tattactcgc
     2221 tctcttgcca taactgcccg gacgtggtgc aggcgctgaa cctgatgagc gtactgaacc
     2281 cgcgcatcaa gcacactgca attgacggcg gcaccttcca gaacgaaatc accgatcgca
     2341 acgtgatggg cgttccggca gtgttcgtaa acgggaaaga gtttggtcag ggccgcatga
     2401 cgttgactga aatcgttgcc aaaattgata cgggcgcgga aaaacgtgcg gcagaagagc
     2461 tgaacaagcg tgatgcttat gacgtattaa tcgtcggttc cggcccggcg ggtgcagcgg
     2521 cagcaattta ctccgcacgt aaaggcatcc gtaccggtct gatgggcgaa cgttttggtg
     2581 gtcagatcct cgataccgtt gatatcgaaa actacatttc tgtaccgaag actgaagggc
     2641 agaagctggc aggcgcactg aaagttcacg ttgatgaata cgacgttgat gtgatcgaca
     2701 gccagagcgc cagcaaactg atcccagcag cagttgaagg tggtctgcat cagattgaaa
     2761 cagcttctgg cgcggtactg aaagcacgca gcattatcgt ggcgaccggt gcaaaatggc
     2821 gcaacatgaa cgttccgggc gaagatcagt atcgcaccaa aggcgtgacc tactgcccgc
     2881 actgcgacgg cccgctgttt aaaggtaaac gcgtagcggt tatcggcggc ggtaactccg
     2941 gcgtggaagc ggcaattgac ctggcgggta tcgttgagca cgtaacgctg ctggaatttg
     3001 cgccagaaat gaaagccgac caggttctgc aggacaaact gcgcagcctg aaaaacgtcg
     3061 acattattct gaatgcgcaa accacggaag tgaaaggcga cggcagcaaa gtcgttggtc
     3121 tggaatatcg agatcgtgtc agcggcgata ttcacaacat cgaactggcc ggtattttcg
     3181 tgcagattgg tctgctgccg aacaccaact ggctcgaagg cgcagtcgaa cgtaaccgca
     3241 tgggtgagat tatcattgat gcgaaatgcg aaaccaacgt caaaggcgta ttcgcagcgg
     3301 gtgactgtac gacggttccg tacaagcaga tcatcatcgc cactggcgaa ggtgccaaag
     3361 cctctttgag tgcttttgac tacctgattc gcaccaaaac tgcataagaa gaagtaagat
     3421 tcacctgcaa ttgcttagcc gccggggtca aacctggcgg cttttttatg gcattaaaaa
     3481 gccctgccgg gatgttccag gcagggcggc ataaatactt ttatctgtta gcgaatgacc
     3541 agtacaggca gattggcgtg gcggatcacg ctcgaggcgt tagaacctaa cagatgggtc
     3601 gaaatcgatg ggttgcgaga accgataact acaacatcag cccccagttc ttctgccaac
     3661 tcattgactt catcccgcac gctaccaaaa cggacatgtt gtttaatgcg ggaaggatcg
     3721 atggtgaagt ggctgaccat cgtttgcaga cgttcttctg cttcatgttg cagatgctct
     3781 tcaaaacgac gcacatcagc ggcaaaacgg tgcaggctca ggctggctga cccgggtagt
     3841 acgtgaagta gatgaataac tccgtcatcc tgggcgagga attcagcgtg gcgaacagct
     3901 ttgtcgctca attccatttc aaatacatca actggcataa tgattgtctt atacataacc
     3961 ctttctccct gttaatcatg aacaaatcat tcgccatgat tataatattt atccctgata
     4021 tttgtctggt tctttttcct tacgaacggt ttctgtgatt aatatattct cactgaacac
     4081 caggaattct cccaaaacct gcggtaccgc ccgttttccc gctgtgatag ctacccttaa
     4141 agactgactc ttttttgaac tgtctctgga ggttgcatga aagcattgac ttatcacggc
     4201 ccacatcacg ttcaggtaga aaatgttccc gatccgggca ttgaacaggc tgatgatatt
     4261 attctgcgta ttacggcaac ggcgatctgt ggctctgacc tccatcttta tcgaggcaaa
     4321 ataccccagg ttaaacatgg cgatattttg ggtcatgaat ttatggggga agtcgttgaa
     4381 accggaaagg acgtaaaaaa tttgcaaaaa ggcgaccggg tggtaattcc gtttgtcatt
     4441 gcttgtggcg actgtttttt ctgtcgaatg cagcaatatg ccgcctgcga aaataccaat
     4501 gcgggtaaag gcgctgcgct caataaaaaa cagataccag ctcccgcggc attgtttggt
     4561 tatagtcacc tgtatggtgg cgttcctggt ggacaggcgg aatatgtccg cgtccctaaa
     4621 gggaatgtgg ggccgtttaa agtaccgcct ttgctttcag atgataaagc ccttttcctt
     4681 tctgatattc tgccaacggc atggcaggca gcaaaaaatg cgcagatcca acaaggttca
     4741 agcgttgcag tctatggtgc cggtccagtg ggattgttga caatcgcctg tgcacggttg
     4801 ctcggtgcgg aacagatttt tgttgttgat catcatccct accgcttgcg tttcgccgct
     4861 gaccgctacg gcgcgatccc gattaatttc gatgaagata gcgatccggc acagtcaatt
     4921 attgaacaaa cggcaggtca ccggggcgtg gatgcagtaa tagacgccgt cggttttgaa
     4981 gcgaaaggca gcaccacgga aacggtgctg actaacctga aactggaggg cagcagcggt
     5041 aaagcgttgc gccagtgtat tgcggcggtc aggcgtggcg gcattgttag cgtaccgggc
     5101 gtctacgctg gatttattca cggtttcctg tttggcgacg cctttgataa agggttgacg
     5161 tttaaaatgg gacagaccca cgttcacgca tggctgggtg aattattacc gttaattgag
     5221 aaaggattac tgaaaccaga agaaattgtt acccactata tgccgtttga agaggccgcc
     5281 cggggatatg agattttcga aaaacgtgaa gaggagtgcc gtaaggtgat tctggtaccc
     5341 ggtgcacaaa gcgcagaggc ggcgcagaag gcggtttcag gtctggtgaa tgcgatgccg
     5401 gggggaacaa tatgatcgtc aggagtggtt ttcgaggtaa aggacagcca tgacgataat
     5461 cgccgccata atcagaaatc ctatcaggat gtaaaatgct tctgccatgg ttattcccac
     5521 aaacgaaacg gaataatttt gcagcaaaag tgaacagtga gaaccaggaa aaattgctga
     5581 ttttgcgtaa agaggatgcg agtgcatcct ctgggcaaag cgagttatcg cttgtgcaga
     5641 tgggattaaa gcaggtagtc gccagcagct tctggctggt attcgagttc cagcacttca
     5701 aggtgggttg caacgccgcc cggaagttcc cagtgaatgg aatcgccaac gcgcagcccc
     5761 agcagtgcgg cacctaccgg agccataacg gaaagctgag tattgctatc ggtcattttt
     5821 gccggataca ccagcgtgcg cacacgcact tcgccatcgc taagattgcg gaatttaacc
     5881 cggctgttca ttgtcaccac gtcgtgtggc atctcttctg gcgaacacat ttgggcgcga
     5941 tccaactctg cgtttaacgc gtcggcgatt ggcaaaccag cataggcggg ttgctccagc
     6001 agaatatcga tgcgttcggc atccaggtcg ttaatgatga tagttggtct ggacattttt
     6061 actccatgtc gtcggtactg cgagtgtcgc agataaacat acccaaaaga aaaccctcac
     6121 cgtcaggcgg cgagggttta actcacatga tgatactgac tgttgctcac tctttgaagt
     6181 gatttgcgtc acattcaggg aattcctcaa tgcagcgcat tatgtataat tcttaatcgt
     6241 cacggtttat ggaagacgaa ttagcgtgtt ttgtaaatca gatgattaat aacccacttt
     6301 atcaatcaca aaggttttgc cacagttgcc tgggtgtggt tgtggcaaga atgagtttgc
     6361 agaaagcgga gcgttgatgg cgtcagcttt gatcgagatc tgaatttcag tcaaatacgc
     6421 agggttaccc tggcacgtta gcttaactgc tttcacgttc tcttttcccc agcttttggc
     6481 aaaggcggcg tcaaagtcac ggcggctcac tgttttaccg taattatccg caaggaattt
     6541 tccggcttcg ctctctttaa tttcttgatt caggcgtacc atcgtaccga agtatgcgtc
     6601 cggatcaaaa ccaaagcagg caccgtgttt ggcatattcg tagcgttcca ggcaggaacg
     6661 tccgccagct cctggcatga cttcacttag tttagcggcc gtttccagtg ataacccggt
     6721 ttccggcgat gaacacatcc ggctggcgtg cgcttctggt agattcggga ttgggcgagt
     6781 agcgcaaccg aagcgcatcc agcggcgttc atcaacacca cgggcagcaa ccgatttagg
     6841 caatcctggc cacagaccat gtacggtcag aaaatcagct ttgttggtcg tttcggtttg
     6901 taggcgacat tcatctcgtt cgttacgatt tcgatcgtgt tgactctggc aaaatccggt
     6961 ttgccaggag agggccagga catagcgatc aaaatcgcca tactgttttg cctgcaacgc
     7021 tacggcgttg gcagaagaga agggaagcag agaaaccgcg agcaacgcgg cgttacgcca
     7081 gaatgctttc ataatgggtg tggaactcat acatacactg aatactatct attaaatcat
     7141 aaaaaagccc gccaggtggg cttacctggc gggcgtgatg atttattcag cgtttggcga
     7201 acgtattagt tccacatggc gagaatcggc cagccaacca acagcaacat agagatgtaa
     7261 atcaccccga agattgcgcc aagacgccag taatcttttg atttcacata gccacagccg
     7321 taaataatca ccccaggacc ggttgcatac ggcgtcagac agcccatgat accgatagac
     7381 agcaccagca ggatacacag ttgttccatt ggtacgcccg gaataccttt accgacggcc
     7441 agaataaccg acagcatggt tgcggtgtgc gcagacaggc tggcaaacag gtagtgtgca
     7501 aagtagaaca ccagaaccag tacaatcacc gttgcgtttg gtgagaatcc ttccaggtgc
     7561 gtactcatgg taccggcgaa ccagtcaata aagccagaac gagtcaggcc gttagccatc
     7621 acaaccagag ttgccaggtt gaccagcgtg ttccatgcgc tgttatagcg ggtaatatct
     7681 ttccaaggca caacgtgcag ggccagcatt agcgaaactg ccagcagacc aactgcagta
     7741 gcattaatga cttcactgcc aaaaacccac aaacctaagc tgagcaatac aaggccaatc
     7801 agtgtccact ctctgcgtgt cagcgcaccc atggttttca gttcatcacc cgcccaggtt
     7861 gccacttctt cactgtgtgt gatttccggt ttgtacagca cgtaggaaag ccacggcgca
     7921 atgataagca agataacccc aaccggcagg aagcagagga accactgcaa ccagctaatc
     7981 tggataccgg caattttgct gacgaactcc agacccagca cgtttggtgc cgcaccggtg
     8041 acaaacatgg acgaactcag actggtacta atgaccatca tccacatcaa atagccgcca
     8101 atacgacgcg cggacggatc gttggggaat gatttaaaca gcggcggcag gtttttaatg
     8161 accggaaaaa ccgtaccccc ggtacgcgcg gtgttggacg gtgtaaacgg tgccagcaga
     8221 atgtcgataa tgacaatcgc ataacccaac gtcagcgtgc gtttgcccat gaatttcacc
     8281 aggaaaaggg caatgcgacg acctaacccg gaaacttcat accctaatgc aaaaataaat
     8341 gcgccaaata ccagccatac cgtggtgctg gaaaaaccag ccagacccca tttcagcgcc
     8401 tgtttttgcg cattaaacgc tgggtcagct aattctttgg catcaaagag caggtaatta
     8461 ctgccaataa cgcaaatagt aaccgcaata aaactgatcg ctgttgccgg aattggctcg
     8521 aggatcatgc cgacaatcat tgccacaaac acagcgaagt aatgccatgc ctgtggtggc
     8581 ataccgtcgg ggacagggat aagaaacatg acacccatca ccaccagtgg ggccaatagt
     8641 ttccatatat tatcttttgc taaagacata cgggttctcc gaaaattaat atttccaaat
     8701 ttatcaagtg cttaaataat taaatctgtg ctaaaaacca ggtaaggatc agcaggtcgg
     8761 cactgccgcc tggactgaga tttcgttcga tacactccct gtcgaactgc cggagataat
     8821 cgagatcggc gggggttcga atgccccctt tttgcaataa tgtttgcgcc tcgcgctgta
     8881 gccagcgcag gcccccctcg ccaccgcgcg atgcaaagtt ggtatcgccg ttgatcgcca
     8941 tcagtaggag caaggtatcg agcaatgcca gttcaggatc taacccctga tccagcagag
     9001 tgaggtaatg cggcaaggcg tgattgatca ccagtggata acccgcttcg gcttcaccgc
     9061 gtgcgccggt aaggccaagc tgctggtaca accgttgacc tgccgtcagt tgtgaattat
     9121 tggtacgcag ttctcgatcg gtcaggccac ggcagaaact tgccgccgta gaacaaacgg
     9181 ttgttggcgt taccggttgg ttgagttgaa gcaaacggcc aattgccgca catagtagcc
     9241 ctaaagaaaa aatgctgcct ttatgcgtgt ttacgcccgc agtggcgcgg aacatatcac
     9301 cttcgcaagc cataccaatt gggcgtaatc cgtggagtac cgcttctggt gctatttccg
     9361 cactacaggc accaaattca atgaaacggg gtagccagcc ctgaatcgcc agcgcgctgc
     9421 ggtggaaatc ttccagcgcc atatctttat gtgcaccgca gttaatccga tccacgaggc
     9481 ctggtttcgg tgacagattg acttcagtca gcatggcgcg ccagcccagc agggcgtact
     9541 catcgatcaa tgacgtcgca agctttgtgg ttttagttga cgttgcaggc atcgacatcg
     9601 ttcagcagtg cctccatgcg gttgagtaaa tcggtcagtt gatgggtttt tccacgcgcg
     9661 cagacggctg cgctttgctc gcacaacagg cagcggcgag gcggcagtga atagtcgcgg
     9721 cgggagagaa tttcgccttc gggcgtcagg acatcgatat cccataaccg cccgagagga
     9781 tgactatgtt caagctcaat ggtggcgagc ttgaggtcgc gagccggggc ggcaatgctc
     9841 aacatgccct ccggcccgct ggcggaaacc agtgcagcct gctcctgaat ttgccagccc
     9901 tgttttgcgg ctaaggcacg caaggctgtc acgccatgat taaaaattcg gcgtgtgacc
     9961 tcgctgtctt taatcggccc aggcgcaacc acggtaaagg agaccagtgg aacaggatgg
    10021 cgcttgagcc agacgtgttg ccgtgcttgc ctttcatccc ggctgacgag cagctcggga
    10081 attgacaccg catggtggct ggcgagttca ggaagcaggt gcatggctta ttccttcacc
    10141 tgatgcacaa catcgatcac cgagccatcg cggtaacgca caacggcaac gacgcggtcg
    10201 gtgaattcaa tcggctgtgg ttcaccggtc agcagacgcg cgcgttcgcg cagccactca
    10261 atggaaacca ctttaatgcc cgcttcctgc agacgttctg ccagttccgg acgtgccggg
    10321 ttaactgcga taccgtggtc tgtgaccaga atatcgacac tggagcctgg ggtgatgcag
    10381 gtcagcacgt tatccaccag agtcggaata cgaccgcgta ccagcggcgc gacgatgatg
    10441 gaaagcgcag aggcaatcgc ggtatcgcag tgaccaccgg aagcaccacg cagtacgccg
    10501 tcagagccgg tcagcacgtt aacgttgaac tgggtgtcaa tttccagcgc gctcagtacc
    10561 accacgtcga gacgatcaac cgatgcgcct ttcgaacccc agttagcgta ctggttggcg
    10621 ctgatttcga tgtgattggg gttacgggcc agcgattgcg cagcatggct gtcaaagctc
    10681 tgcacatcca gcagtttgcg gatcagacct ttttcgtgca gatcaaccat cgtcgcggta
    10741 ataccgccga gggcgaagtc ggcgcgaata tcgcggctac gcattttgtc ttccaggaaa
    10801 cgggttaccg ccagcgatgc gccgccggtg ccggtttgca tggagaaacc ttctttgaag
    10861 tagccagagt tgacaatcac atccgcagcg ctacgggcaa taagcagttc gcgcgggtta
    10921 gtggtcatac gggtcgcgcc agcgccgatt tttgcagcat cgccaacgcg gtcaactttg
    10981 acgatcaaat caacctgatc ttgctcaatg cttgccggat tatgcggata aggcagcagt
    11041 tcttcggtaa gcatcacaac ctgttttgcg ttgtcggcat caaccattgc atagccgagg
    11101 gagccgcagc aggctttacc ggtgtaaccg ttggcattac cgaattcatc acaggacggg
    11161 acgccgagga aagccacgtc gatattcagt tcgccgctct gtaccagatg cacacgtccg
    11221 ccgtgagagt ggatttgcac cggttccgca agcagaccac gggaaatttc ttctgccagt
    11281 ggaccgcgca ggccggaggt ataaatgcgg gtaaccacgc cctggcgaat gtgttctacc
    11341 agtggcgcat ggcaatcact cagggagctg gacgccaggg tcaggttttt aaagcccatc
    11401 ttagcgatga cgtccatcac catattgacg gtcaggtcac cgccacggaa agcgtgatgg
    11461 aaggaaaccg tcatgccgtc ctgtaaacca gagcgacgaa tcgcttcttc ctggttggcg
    11521 cacagtttgc gatcgcgcgc tttttcagcc tggtaggttt gctttggtga gttctggaaa
    11581 gcggcaagat cgcattcagc gcgacgattc caggccgcta cccgttcttg tcgttgagat
    11641 tgttcaattt tctgcgtcat tttgattgcc ttattcttcg cggatgccgg aaagttctgc
    11701 acgggagagc accagacggg cacgatcgat aaccggaccg tccaccatct tgccgttcag
    11761 ggaaaccacg ccgaggcctt cgcgagcggc ggcttcagcg gcttctacga cgcggcgggc
    11821 gtgatccact tctttctggg tcggtgcgta gaggttgtgc aacagatcaa tctgacgcgg
    11881 gttgatcagc gatttgccgt caaagcccag ctgtttgatg tgggcggctt cttgcaggaa
    11941 tccggcttcg ttgttagcgt cggaatagac ggtatcgaac gcctgaatac ccgcagagcg
    12001 cgcggcctgc aaaatggaac agcgtgcgaa cagcagttca gttccttccg gggagcgttc
    12061 tgtacgcagg ttgcgcacat agtcttctgc accgagggcg ataccgatca aacgctcgga
    12121 agcgtgagcg atttccactg cgcgggtaat ccccagcgga gattcaatcg ccgccagcag
    12181 gccggtgctg ccgggttcac gaccacaggc tttttcgata cgcaggatct ctttttcgat
    12241 atccagaaca tcctgagcgg tatcggtttt cggcagacgc acaacgtccg caccaccgcg
    12301 aacgacggct tccaggtcgt tgacacccca ttcggaatcc agcgcgttga cacgcacaat
    12361 ggtttcaata tcgcgataca gcggatgttg cagcgcgtgg taaaccatgc ggcgggcggt
    12421 gtctttttca cgcaatgcta cggagtcttc gaggtcaaac atcagggcat cagccgggta
    12481 gatgaaggag ttgctgacca tcgcggcatt ggcaccaggc acaaacaaca tgctgcggcg
    12541 ggtgcgagtt ttacgttgtt gcagcgaagc ggaaatcatt ggcaatcctc ccatggcaga
    12601 gccgggatac cgctggcgcg tgccagcagg gcttccagtc gtgcacgtaa aatgcagtcc
    12661 agtgcgcctt tgtcatcgac attcagctgt acgccgcgca cgttgtagcg ggcgagaacg
    12721 tccagaatgg tggtgcgaat tgcatcgcca aactgtttct caacgctgct attgatttgc
    12781 aggtcgatat cctgcgtatc gagtggggcg atgcgtatca tcacatcccc agactcaagg
    12841 gtgcctgcaa cggcgggctg gtttattttc atttttcacc tgtttctcat gcgggggtct
    12901 tttgacgagc tgccgcgtcc tggcgggagt gctcaagcag gttctgcaaa taatgcagcg
    12961 tgactgcagg gaccagcggc gcgatagccg tgagatcgtt tttcgccagc agttgacgta
    13021 cccgggaagc ggatatcggc atctcctggt aacgcagccg ctcaatttca accagttcga
    13081 tgggcggtgc ggagatagtc ggcgtttcca gccagtagcg catatcctgg ttgtactggg
    13141 cggtaacgcg acaaaagggt tcagtaccga caaagcggtg agttacaccc agcgcgggag
    13201 cgaggtactg acggaaaatc ttcagatcaa tttcggtgta acaatggtta atgacgctct
    13261 gttctttaat gaagtagcaa gggaacgtag cgcgggagat gatgtattcg gagccgcgat
    13321 gcacagtcag gcgtggaata tcggcggtgc cttttaacac cagatccaac cggtcttcat
    13381 aggggaagcg tgaagaatct tctttgacta aaaacagatg caaccagtcg cactgtgccg
    13441 cagcttgttg aatcagataa cggtgaccat tcgtgaaggg attggcgttc atcacaatgc
    13501 agccaatctt gttccctgga tgacgaaatt ttttcagcga ttcggcatag cgtttcagcc
    13561 gcgtggcgct gttttccatc agcaccatca cgccgggtac gctggtcagc gtggaaaaac
    13621 cgcactggcg gaacagcgcc tcgtattcgg ttttggtata aataaacaga tgcgtgctgt
    13681 gccgctcata ggcgaggttt atcaattcag tggctaatgt cagcgccagt ccttcaccgc
    13741 gaacggattc actgatagca acgcatttaa taatatttcc ggcaattcca ccgcacgtga
    13801 taagcttttc atcgcgggtt acggtaataa atacttcgac tgtggtgtca acg
//
LOCUS       DAZPEP010000110        13845 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZPEP010000110 DAZPEP010000000
VERSION     DAZPEP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13845
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.110"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..80)
                     /locus_tag="ACHUT9_003042"
     CDS             complement(<1..80)
                     /locus_tag="ACHUT9_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349457.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGT"
     gene            255..2021
                     /gene="adeD"
                     /locus_tag="ACHUT9_003043"
     CDS             255..2021
                     /gene="adeD"
                     /locus_tag="ACHUT9_003043"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFR1349458.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHUT9_003044"
     CDS             complement(2066..3457)
                     /gene="uhpT"
                     /locus_tag="ACHUT9_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFR1349459.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHUT9_003045"
     CDS             complement(3595..4914)
                     /gene="uhpC"
                     /locus_tag="ACHUT9_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFR1349460.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHUT9_003046"
     CDS             complement(4924..6426)
                     /gene="uhpB"
                     /locus_tag="ACHUT9_003046"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFR1349461.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHUT9_003047"
     CDS             complement(6426..7016)
                     /gene="uhpA"
                     /locus_tag="ACHUT9_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFR1349462.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHUT9_003048"
     CDS             complement(7091..7381)
                     /gene="ilvN"
                     /locus_tag="ACHUT9_003048"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1349463.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHUT9_003049"
     CDS             complement(7385..9073)
                     /gene="ilvB"
                     /locus_tag="ACHUT9_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFR1349464.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVEE"
     gene            complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHUT9_003050"
     CDS             complement(9179..9277)
                     /gene="ivbL"
                     /locus_tag="ACHUT9_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFR1349465.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9842..9931
                     /gene="tisB"
                     /locus_tag="ACHUT9_003051"
     CDS             9842..9931
                     /gene="tisB"
                     /locus_tag="ACHUT9_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFR1349466.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHUT9_003052"
     CDS             complement(10055..10129)
                     /gene="ysdE"
                     /locus_tag="ACHUT9_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFR1349467.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10211..11395
                     /gene="emrD"
                     /locus_tag="ACHUT9_003053"
     CDS             10211..11395
                     /gene="emrD"
                     /locus_tag="ACHUT9_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFR1349468.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11403..11900)
                     /locus_tag="ACHUT9_003054"
     CDS             complement(11403..11900)
                     /locus_tag="ACHUT9_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFR1349469.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQEMPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11897..12259)
                     /locus_tag="ACHUT9_003055"
     CDS             complement(11897..12259)
                     /locus_tag="ACHUT9_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFR1349470.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12249..12596)
                     /locus_tag="ACHUT9_003056"
     CDS             complement(12249..12596)
                     /locus_tag="ACHUT9_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFR1349471.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12705..13154
                     /gene="yidI"
                     /locus_tag="ACHUT9_003057"
     CDS             12705..13154
                     /gene="yidI"
                     /locus_tag="ACHUT9_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFR1349472.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13201..13359)
                     /locus_tag="ACHUT9_003058"
                     /pseudo
     CDS             complement(13201..13359)
                     /locus_tag="ACHUT9_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13403..>13845)
                     /locus_tag="ACHUT9_003059"
     CDS             complement(13403..>13845)
                     /locus_tag="ACHUT9_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1349473.1"
                     /translation="VSKNHAMIVIEDLKVSNMSKSAAGTVSLPGRNVRAKSGLNRSIL
                     DQGWYEMRRQLAYKQLWRGGQVLAVPPAYTSQRCVCCGHTAKENRLSQSKFRCQVCGY
                     TANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3283 a         3836 c         3590 g         3136 t
ORIGIN      
        1 gtcccatgct gaggtagttt aaataatcgc gaaagcgtcc ctgattcatt actcacgtaa
       61 tcggtattgt cattattcat ttttttgtcc attggaggag atttaatccc ttgcttttgt
      121 aaaacaaatg acaacaacgg ttcagtgata attatcacat ttcaattgca cattaatgga
      181 tattctttaa taatctcgcg acgtttcttt atgataaata ataatcaaat tgataaaatc
      241 aaaatgagaa aaatatgaat aattctatta accataaatt tcatcacatt agccgggctg
      301 aataccagga attgttagcc gtttcccgtg gcgacgctgt tgccgattat attattgata
      361 atgtctctat tctcgacctg atcaatggcg gagaaatttc cggcccaatt gtgattaaag
      421 gacgttacat tgccggtgtt ggcgcagaat acgctgatgc tccggctttg cagcggattg
      481 atgctcgcgg cgcaacggcg gtgccagggt ttattgatgc tcacctgcat attgaatcca
      541 gcatgatgac gccggtcact tttgaaaccg ctaccctgcc gcgcggcctg acgaccgtta
      601 tttgcgaccc tcatgaaatc gtcaacgtga tgggagaagc cggattcgcc tggtttgccc
      661 gctgtgccga acaggcaagg caaaaccagt acttacaggt cagctcttgc gtacccgccc
      721 tggaaggctg cgatgttaac ggtgccagtt ttacccttga acagatgctc gcctggcggg
      781 accatccgca ggttaccggc cttgcagaaa tgatggacta ccctggcgta attagcgggc
      841 agaatgcgct gctcgataaa ctggatgcat ttcgccacct gacgctggac ggtcactgcc
      901 cgggtttggg tggtaaagaa cttaacgcct atattactgc gggtattgaa aactgccacg
      961 aaagttatca gctggaagaa ggacgccgga aattacaact cggcatgtcg ttgatgatcc
     1021 gcgaagggtc cgctgcccgc aatctcaacg cgctggcacc gttgatcaac gaatttaaca
     1081 gcccgcaatg catgctctgt accgatgacc gtaacccgtg ggagatcgcc catgaaggac
     1141 acatcgatgc cttaattcgc cgcctgatcg aacaacacaa tgtgccgctg catgtggcat
     1201 atcgcgtcgc cagctggtcg acggcgcgcc actttggtct gaatcacctc ggcttactgg
     1261 cacccggcaa gcaggccgat atcgtcctgt tgagcgatgc gcgtaaggtc acggtgcagc
     1321 aggtactggt gaaaggcgag ccgattgatg cgcaaacctt acaggcggaa gagtcggcga
     1381 gactggcaca atccgctccg ccatatggca acaccattgc ccgccagcca gtttccgcca
     1441 gcgactttgc cctgcaattt acgcccggaa aacgctatcg ggtcattgac gtcatccata
     1501 acgaattgat tacgcactcc cactccagcg tctacagcga aaatggtttt gatcgcgatg
     1561 atgtgtgctt tattgccgta cttgagcgtt acgggcaacg gctggctccg gcttgtggtt
     1621 tgcttggcgg ctttggactg aatgaaggtg cgctggctgc gacggtcagc catgacagcc
     1681 ataatattgt ggtgatcggt cgcagtgccg aagagatggc gctggcggtc aatcaggtga
     1741 ttcaggatgg cggcgggctg tgcgtggtac gtaacggcca ggtccaaagt catctgccgt
     1801 tgcccattgc cggcctgatg agcaccgaca cggcgcagtc gctggcggaa caaattgacg
     1861 ccttgaaagc cgccgcccgt gaatgcggtc cgttacccga tgagccgttt attcagatgg
     1921 cgtttctttc tctgccagtg atccccgcgc taaaactaac cagtcagggg ctatttgatg
     1981 tcgagaagtt tgccttcact acgctggaag tcacggaata ataaaaaagc ccggcgtcat
     2041 gccgggcaaa agtcaccagt tacgtttatg ccactgtcaa ctgctgaatt tttttctcgc
     2101 ggcggatttt gcgttcttcc atcaccgcca ctatcgccat caggcagata caaccaatcg
     2161 cggcgatatc aagtgcggcg aaggtgcctg cccagccggt aaggccgaat accggcgtcc
     2221 catcggcaat cattcccaga cctaacttgg caaagctgtc accaatcagg taagcaaagg
     2281 tgcctttaat accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca
     2341 caccaatcaa caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccagat
     2401 agatatattc gttactggca tgttgataca caccgagcgt ggcgataatc agcgccagcg
     2461 cgatgcaggc caccaggcca cggcgaccgt tcgctaggtc agagagccag ccccacagca
     2521 gcgtaccgac cagcgcacca gcttcaaaca gcgtaaagcc ctgaatcgcc accgctttag
     2581 agagtttcag ttcctggaac gcgtatacgg ttgaccactg gtcgatacca atacgtacca
     2641 catagaggaa aatgttggcg aagcacagca gccagatcac tttgtttttc agcacatact
     2701 caacaaagat ctgccacttg gtcatatcgg tagattctgt ctctttgtcc tcttcgctga
     2761 tctcctcgcc gaacagttct tcagctttgc cgaggccata agattccggg gagtcgctgc
     2821 cgtaacgcag gccgataaaa ccgacaatca gcgcgataat cgacgggaag ataaacatgc
     2881 cgatgacatg gccatcgaac aggtaatttg ccccgaacag cgccacgcct gctgcgcctg
     2941 caccgccaag gttgtgagaa atattccaga aaccgaggaa tgtcccgcgt ttacgacgcg
     3001 gcgtccattt ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc
     3061 ttaaggcgta gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac
     3121 tgaagcccag catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt
     3181 ttttgccgtc ggcgtagtag gaaaccagcg ttttacccac gccataagtg atggagaaac
     3241 ccaggccgat catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct
     3301 gcgcgatgtt aaagttcttg cgaatcaggt acatcgtcag gtagccgata aagaccacca
     3361 ggtaggattg catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca
     3421 gggtcggctt gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc
     3481 tgcgtgaggc atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca
     3541 aaacctggaa aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc
     3601 ttcgcgcggt gtctgggcgt tcaaaaaggg cagtaacagc agtgcggaaa tcccggcggc
     3661 gatagagata accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg
     3721 ccaaccagca agcgacgccc ccagataagc aaacaagccg acaaaccccg tcgccgcccc
     3781 tgccgcctct ttgtgggaac actctgccgc cgccataccg attaacatct gtgggccaaa
     3841 gacaaaaaaa ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat
     3901 cagccacagg gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc
     3961 tcggttgccg ttaaacaatt tgtccgagcc ccaacccgct accagcgcac cgataaatcc
     4021 acccagttca aacatcgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcgtctc
     4081 ggacatatac aaattgcccc agtcgttgat cgccgcccgg accacataga ccagcacata
     4141 gcaaaacgaa agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt
     4201 acgcgtcagc cctgcccctt cttgttgttg agctatctcc agcgcgtcgt gtcgccattc
     4261 accgaccgcc ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat
     4321 tcccacgacg atcgccatac aaccagcaat catcatcccg gcacgccagc cgtaatgtag
     4381 tgcagccgct gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca
     4441 taatgcccac caaccaccgc gctcggtacg tgaataccag gccgttaaca gacgcgcaca
     4501 caccggtgaa ccccagccct ggaaaaaggc gttcagcacc cagagtacgg caaacgccca
     4561 tagcgacgtc gagaagccaa acagaatgtt gataatgccc gtggcgataa gccctatccc
     4621 cataaaataa cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc
     4681 ataggtaatg taaaacaggg tcgctaacag gccgatatcg ctacggctga gcacgccgtt
     4741 agcaaggatt tctggtacgg cggcgttaaa acttttccgc gtgaagtaaa acagcgcgta
     4801 acccagccag atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc
     4861 atatttatca gccattaatg gcgcatcggc aggcgctttc agaaacggca acatcatcaa
     4921 accttagaca tagcgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat
     4981 gtgtaatgtg ccacccagcg ccgttacgcg ctcgcgcatt ccggtgaggc caaaaccttg
     5041 ttgcccggaa cccggcggca aaccgctgcc atcgtcttca ataaccagca tcaaccgttc
     5101 atcctgctgc cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt
     5161 cagcccttcc tggcagacac gaaacagcgt cacgcgctgg ttttcgctta acgctgattc
     5221 atcgattcgc cattcgagat ggctgacaat accgcgccct tccagctcca tttcccgcat
     5281 cagtgagcgg atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc
     5341 caacaaacgg cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gttgcccgct
     5401 ctgcttcacg ctggcgttat ctgccgccag ccgctgaaca atgcccgcct gagtacgaat
     5461 agcagtgatg gtctggccga tatcatcatg cagctcacgc gccacatcac ggcgcacgct
     5521 ctcttcggtt tccagcaacc gttcagccag atgctgattg cgcgccagtt ccttttgcag
     5581 cgactggtta agttcacgca accgctggat gccagcgcca agcaacaacc ctgtcagact
     5641 ttgcaccagc agcgagagca ataaatccac cggatgatcg cgccaggttt gactggcgat
     5701 cagcgcgatg gcgttcatca acgtcgcaat cagcgcccct tgccaaccat agtgccaggc
     5761 cagcgcgata atcggcagca ccagacagaa tggcgtaaag cgcgacagtt cgtccggcaa
     5821 tcccaactgg agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc
     5881 gcgccagttg attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc
     5941 gagatagtgc cagaacacca gacatatcgg ggccagcgtc aggccgccag ttaaagtcag
     6001 caacagcgca ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc
     6061 cgccgccgtt aacgccgccc cctgtaacag caaggtgcgc cagtcacgct gatggcgata
     6121 gcgcgagatc agcgctacgg gcagtaacgt cagtaaacta ccgatcatca ataacggaaa
     6181 atgggttaaa ccgaccgcct gcgttagcca gtaaatcagc agccactccg cacccagcaa
     6241 tacgggccag tatccgcgcg ggcattgcag cattagcccc agacgcagac caaacggaaa
     6301 taacagcacc gccatatcag ggcgctcaac cagatgcagg ctgatactcc acaggcaaaa
     6361 ccatgcggca gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt
     6421 cttcatcacc agccatcaaa catgcggcgc gccagctcta cgtcgttact gacgcccagt
     6481 ttttccatca gattggcgcg atggacgtgt accgttttcg gtgacaagcc cagttcggcg
     6541 gcaatctctt tcaccgccat tccttgcgcc agtttttccg ccacctggcg ttcacgtttg
     6601 gttagcgggt cctgacgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag
     6661 ccacccgtgg caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggaa
     6721 agaaagccgc gtgcccccgc gttaagcgcc tgctcaacca gcgcaggact gtcgtgaacg
     6781 gagagcataa tcgtcgccat acctttcggc agctggctta gcagctccag accggagata
     6841 tcgggcatgg agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc
     6901 gcctcgcgcc ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc
     6961 tgcgcaaagc cggagcggac gatgaggtga tcgtctataa gggcaacggt gatcatggtc
     7021 ttgtcctggc gggtaaaaaa aaacgcgctt accttaacga taagcgcgat gttgttcaag
     7081 ccttgagcgg ttactgaaaa aacaccgcga tcttgttaaa catcgtcgga tcggactgat
     7141 tacgctgcac tttcacgaca tcttccagct tatcgatttg gcttatcatc tgctccagac
     7201 gctggtcgtc attgaccagt agccagatat ggcttttgtc gctgtcctga atcggcagac
     7261 aaagaatgcc ttcaacgtta aaagcgcggc gggcaaaaag gccacaaacg tgggtcatta
     7321 cgcccggatg gttgcgaacg gtgagctcca gaattacgtt gtcatgagtt gtgttttgca
     7381 tggcttattc ctccaccatt tcagtattcg ccgcacctgg cggcaccatc gggtaaactt
     7441 tttcttcggc atcaatgcgc acatggatca gcgccgggct agggcgattg atgatttcct
     7501 gcaatgcagc ctgcggatcg gcttcgttat tcaaatcaca ggtttcgagg ccgaatccgg
     7561 cggcaatctg cataaagttg attttgcccg gataggtggc ggcaaaaacg ccttgctcgt
     7621 agaacagact ctgttgctga tgcaccagcc ccagcgcttc gttgttcatc agaatgattt
     7681 tgacatccag ctgattttca ctggcggtcg ccatctcctg aatattcatc atcaggctgc
     7741 cgtcgccgga gaaacacaac actttgcgat ccgggttcgc cagcgccgcg ccaatcgccg
     7801 caggcaggcc aaaacccatc gtgcccagcc caccggaggt cagccactgg cgtgggcgat
     7861 tgagcggata agcttgcgcg gtccacatct gatgctgacc cacatcggtg gtgataatcg
     7921 cattgtcatc gacacaggcg gcaacggcgt tgatcaggcc gtaatggctt aatggatcgc
     7981 acgctttcgg gattggacac gggaattcgc gctgcaaatc cgctaccaac tggtgccact
     8041 ctgcacgcgg ttgcgcttcc acctgcggga tcaattgcgc cagcacgtca tcaacatccg
     8101 cctgaatcgc cacatgcggc tgcttgattt tacccagctc tgcgcgatcg atatcgacat
     8161 gaataatttt ggcattcgga cagaactgct cggttttgcc aatcgcccgg tcatcaaaac
     8221 gcgcaccgag cacaatcaac aaatccgcct cctgcaaaat atagttggta ctgcgcacgc
     8281 cgtgcatccc cagcataccc agcgacaacg gatgcgcttt tggcagcatg cccagcgcca
     8341 ttaaagtcat ggtggtaggc agttgcgctt tctccgccag ttcacgcacc cgcgcaggcg
     8401 cattgatcac accaccaccc agataaagca ccgggcgttt ggcagcgtta atcattgtag
     8461 ctacgtcacg aatgctttct tcgctaaagg cgggtgcagc ggctttttct gccaccgcgg
     8521 gctgagcttc aatctcaaaa accgccgttt gtacatcctt aggaatgtct atccacaccg
     8581 ggccagggcg gcctgattgc gcaatgcgga aggcatcgct catgacctgc gggagttctt
     8641 cgatatgtct gaccagatag ttgtgtttgg tgatggggat agagatgccg taggtgtcga
     8701 cttcctggaa ggcgtcggtg ccgatcatcg aggcgggaac ctgaccagtg atgcaaatca
     8761 gcgggatgga gtccagccgc gcatcggcaa tggcggtcac caggttagtc gcacccggtc
     8821 cgctacaggc catacagacc gccggtttac cgtcggtgcg cgccattccc tgagcgataa
     8881 agcccgcgcc ctgttcatga cgggccagaa tatggcggat ttgcgtgctt tggcttaagg
     8941 catcgtaaac aggcaggata gaaccgcccg gaatgcctgt cacaatctta atgccctgct
     9001 gttccaggaa atgaacgata aattctgcgc cggtaaagcg cttacgcgtc aatgttgtgc
     9061 ccgaacttgc catgctccag tccttttctt ctgggccgac tttccgggag gtgcttaaac
     9121 gaaaaacccc gcccggtttg cgccgacggg gttttggaat cgtgtgttgt tccagtccct
     9181 acggcgcatt gccgacgacc accaccacac gcacgacgac cactgcggcg gatggcgcag
     9241 ttggtagtag ttttgcgttg aacatggaag tagtcattgg ggaccttgtt ggttttgtgt
     9301 ttaacaatat ttatacaagc acagctttac aggggagaca atggaaaatt tttcagcaag
     9361 ggaaaattga ggggttgatc acgttttgta ctgaattgca gataacaaaa aaccccgccg
     9421 gagcgaggtt tcgtcagtcg cccgcggctg gtaaccgcaa agcacactgt attatgtcaa
     9481 cacagaaagt atacgtgttc cgcgcagaac gcgcaatttc ggcacgaatt ttgacgtatt
     9541 tagtgcatag ttgagtatcg atcacagttt gcgttttgtc caaatattac tgtttattta
     9601 tacagtaaac ttctataata tcactgtacg caatgtgtta tgcgggggcc gcatcgttac
     9661 ccggcgcact aagtcctggc tgaaacgggt ggtgccgtca gcgccttaac cccgcgtgag
     9721 cacactgtgt tatgtcaaca agcacaaagt ttctccttga gataccgcgt gcacaacagc
     9781 tggcaacagg cagcggaaag gtacgtcagc tggcagtgct cctgaaccac aggagacgcg
     9841 tatgagcctg gtggatatcg ccattcttat cctcaaactc attgttgcag cactgcaact
     9901 gcttgatgct gttctgaaat acctgaagta attcagattc aagtcgcacc aaaggggagc
     9961 gggtaaccgc tcccctttta tatttagcgt gcgggttggt gtcggatgcg atgctgacgt
    10021 atcttatccg ccctaccatc tctcccggca acatttattg ccgcttttgt ttacatattc
    10081 tgccgctaaa caactcccca ttcctggcgt atatctggct aacattcatc aatgtgatag
    10141 attcctctcc cgcatttatg ggaatgcgta gtgacttact ctaattattt ttataaaagc
    10201 atccgtgata atgaaaaggc atagaaacgt caatttgtta ttgatgttgg tattactcgt
    10261 ggccgtcggt cagatggcgc aaaccattta tattccagct attgccgata tggcgcgcga
    10321 tctcaacgtc cgtgaagggg cggtgcagag cgtaatgggc gcttatctgc tgacttacgg
    10381 tgtctcacag ctgttttatg gcccgatttc cgaccgtgtg ggtcgccgac cggtgatcct
    10441 cgtcggaatg tccattttta tgctggcaac gctggtcgcg gtcacgacct ccagtttgac
    10501 ggtattgatt gccgccagcg cgatgcaggg gatgggcacc ggcgttggcg gcgtaatggc
    10561 gcgtactttg ccgcgagatt tatatgaacg gacacagttg cgccatgcta acagcctgtt
    10621 aaacatgggg attctcgtca gtccgttgct cgcaccgcta atcggcggtc tgctggatac
    10681 gatgtggaac tggcgcgcct gttatctctt tttgttggtt ctttgtgctg gtgtgacctt
    10741 cagtatggcc cgctggatgc cggaaacgcg tccggtcgat gcaccgcgca cgcgcctgct
    10801 taccagttat aaaacgcttt tcggtaacag cggttttaac tgttatttgc tgatgctgat
    10861 tggcggtctg gccgggattg ccgcctttga agcctgctcc ggcgtgctga tgggcgcggt
    10921 gttagggctg agcagtatga cggtcagtat tttgtttatt ctgccgattc cggcagcgtt
    10981 ttttggcgca tggtttgccg gacgtcccaa taaacgcttc tccacgttaa tgtggcagtc
    11041 ggttatctgc tgcctgctgg ctggcttgct gatgtggatc cccgactggt ttggcgtgat
    11101 gaatgtctgg acgctgctcg ttcccgccgc gctgttcttt ttcggtgccg ggatgctgtt
    11161 tcctctggcg accagcggcg cgatggagcc gttccccttc ctggcgggca cggctggcgc
    11221 gctggtcggc ggtctgcaaa acattggttc cggcgtgctg gcgtcgctct ctgcgatgtt
    11281 gccgcaaacc ggtcagggca gcctggggtt gttgatgacc ttaatgggat tgttgatcgt
    11341 gctgtgctgg ctaccgctgg cgacgcggat gtcgcatcag gggcagcccg tttaagcgca
    11401 cgtcaccgca gcatcgtcat cagctccatg ggagaacgat gctgctttat cagatcacgc
    11461 atcacccgca tatgcggtgc ggagtaagaa taaaacgcct gatagcccgc acaaagcacg
    11521 ctgtctggca tctcctgccg agcgtgcggg cacaggcgat gccagtcgca cgcctggcag
    11581 tcacagcgcg gttgcgggtt ttcgctgcca caccattttt gtaaggtggc gtcaaaaagc
    11641 tcaatcgaga tacgactgat atctgcccgc acccaacact cgaacagggc aattaaaaat
    11701 ttcccccaca gctctgctgt caccgaagcg gccgtcagat cgccccggtc atcaaattca
    11761 agatagggaa tgagctgtac ggagcggatg ccccgctgtt tgaaattgtg ataaaaatcg
    11821 gcaggttgca gagcatcctt gtactctaca accagtttat gcctgtcctc ttcagcatca
    11881 atgacctgac ttcctgtcat gcgactctct caataaatac gataagttga tgtatatgcg
    11941 ttacagcaaa cagtattgcg agagataaca ccgcgaggga gatcaaaaat ttgtcacgga
    12001 ctacgtgaaa ttgggaaaaa tcgctattcg tgacatccat taaattgcga ttacgggtgt
    12061 agtgccagag gatcagcgcc acgatggcga gaatgccaat cgaaatccag aataacatgc
    12121 ccgcctggtg ccagttgtgt ttgatagcca acgccatcaa cgcgccgtag cccagcatgg
    12181 tacgaaacca cgccagtgat gtgcgctccg gctgcagtcc tgggtcggca atgcgtctgg
    12241 ctttgcggct atccggcata caacaccagt cccataacga tcaccgccac gaccatcaat
    12301 attaagctaa tgattaataa gctgttggtg tagggcaggt cctctttcag acgcatcgct
    12361 ttttcattgc gtaaccagcg caggtagcca tacattgcta acccgccgga aaacagacac
    12421 aacaacaacg ccagcagttc acgaatgacg ggcgtggcga aatctggcgc aagttgatca
    12481 agcccaaccc ccgccgccag aaatcctagt gcggtacgga tccacgccag aaaagtacgc
    12541 tcattagcca gcgagaagcg gtaatcgggc gcttctccaa ggcgggaaat tttcattgtt
    12601 gctcctttgc aatttcacta actctctacc tttagcggtt ttttttaaga atttcgtccg
    12661 cccgtttcct gatatgcttt tgccatccgc tacaaggagg tcagatgggt atcatagcgc
    12721 aaaataaaat cagttcattg ggcatgctgt tcggtgccat cgctttaatg atggggatta
    12781 tccacttttc attcggccct ttttccgcac caccgccaac gcttgaaagc atcgtggcgg
    12841 acaaaacggc tgaaattaaa agaggtcttc tcgccggaat aaaaggcgaa aaaattacca
    12901 ccgtggagaa aaaagaggac gtagatgtcg ataaaatcct cgatcaaagc ggtatcgcac
    12961 tggccattgc tgcgctactt tgcgcatttg ttggcggtat gcgtaaggaa aatcgctggg
    13021 gcattcgcgg cgcgctggtt tttggcggcg gaacgctggc tttccatacc ttgctttttg
    13081 gtatcggcat cgtgtgcagc attttgctga tattccttat cttttccttc ctgaccggcg
    13141 gttcgctggt ttaagcagcc tgaataatgc ccgccgggtg atcatcagcg ggcattatcg
    13201 tcagaacttc tgttttttct cttccacctt caccccttgt gtcggtaggc cggtgtcata
    13261 gtcacgtacc accggcgaat agccatcttg tgggcgtgga cgaaatgccc ccatccagcg
    13321 cggccgtgca tctttacgcc acggacgcag gctccattgt tgacatcctc cacgccctga
    13381 aggacgtgga cccctgctac gttcaggctg tcgcctgaat catttcggtg ggttcctgct
    13441 tcaacgagcg gcctgactgc accatctctc cacaggcaag aacggcgtgc cccgccgcta
    13501 aaatgttacg agcgccattt acatcggcgt tcgctgtata tccacatacc tggcatctga
    13561 atttactttg tgacaggcga ttttctttcg ctgtatgacc acagcacacg caacgctggc
    13621 ttgtgtacgc tggcggaaca gcaagcacct gaccaccacg ccagagctgc ttgtacgcaa
    13681 gctggcggcg catttcatac cagccctgat ccagtatcga acggtttaaa cctgattttg
    13741 cccggacatt gcgccccggc aggctgaccg tacccgctgc tgactttgac atgttgctga
    13801 ccttcaaatc ctcaatgact atcattgcgt ggtttttgct gacgg
//
LOCUS       DAZPEP010000111        13675 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZPEP010000111 DAZPEP010000000
VERSION     DAZPEP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13675
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.111"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            79..1581
                     /locus_tag="ACHUT9_003060"
     CDS             79..1581
                     /locus_tag="ACHUT9_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFR1349474.1"
                     /translation="MNKKLYLFPDSNIFLQCKDLTQVNFSEITACDEVCIIITRPIQQ
                     EIDRQKGQGNSRLSKKARKAASLFNQVVDSTDMTLVIRERSPRVFLTMDLSLKPCEQL
                     SEQLDYQEADDRFVGIAAGYCADDPDSEVAIITNDSGPRFSAIKHNIICYKVPSSWLL
                     PAEPDEKDKQIKLLEAKLKQFTQSMPDFSIKVADDEQLNIVLPYYTALSEYEVDNLLC
                     QLTDAFPLVTNFDNEQTTPQSLEKIIKSFSQQEYFPVSADDISLYKEKHYPLWKKQCE
                     EILKNCHTHINPKAINYPLSFLLQNTGYTIAERAVVTFTAKGDFRLCGFNWEIMEELT
                     ESKRIDLPSAPTAPKGKWRSVCGVTERMPYAGGALETPLSSIISKEIGPLLSLTPTER
                     DKNDFYYKEKRNEPVQVVSLECEEWRHQVKEEIFPLFVYTANKPKKVNGAIEVRVDAN
                     NLTNPVIKTFKLKITIEEHSAFSTITEMVQTYIQEQKKRQKSSISAQIKT"
     gene            1825..2085
                     /locus_tag="ACHUT9_003061"
     CDS             1825..2085
                     /locus_tag="ACHUT9_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFR1349475.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEDIPWHDLPASHRGNNQMSLPLPQN"
     gene            2276..2416
                     /locus_tag="ACHUT9_003062"
     CDS             2276..2416
                     /locus_tag="ACHUT9_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1349476.1"
                     /translation="MSQKSLTTITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(2723..3022)
                     /gene="ihfA"
                     /locus_tag="ACHUT9_003063"
     CDS             complement(2723..3022)
                     /gene="ihfA"
                     /locus_tag="ACHUT9_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFR1349477.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(3027..5414)
                     /gene="pheT"
                     /locus_tag="ACHUT9_003064"
     CDS             complement(3027..5414)
                     /gene="pheT"
                     /locus_tag="ACHUT9_003064"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFR1349478.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            complement(5429..6412)
                     /gene="pheS"
                     /locus_tag="ACHUT9_003065"
     CDS             complement(5429..6412)
                     /gene="pheS"
                     /locus_tag="ACHUT9_003065"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFR1349479.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     misc_feature    complement(6621..6745)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            complement(6695..6739)
                     /gene="pheM"
                     /locus_tag="ACHUT9_003066"
     CDS             complement(6695..6739)
                     /gene="pheM"
                     /locus_tag="ACHUT9_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFR1349480.1"
                     /translation="MNAAIFRFFFYFST"
     gene            complement(6862..7218)
                     /gene="rplT"
                     /locus_tag="ACHUT9_003067"
     CDS             complement(6862..7218)
                     /gene="rplT"
                     /locus_tag="ACHUT9_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFR1349481.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     gene            complement(7271..7468)
                     /gene="rpmI"
                     /locus_tag="ACHUT9_003068"
     CDS             complement(7271..7468)
                     /gene="rpmI"
                     /locus_tag="ACHUT9_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFR1349482.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            complement(7565..8107)
                     /gene="infC"
                     /locus_tag="ACHUT9_003069"
     CDS             complement(7565..8107)
                     /gene="infC"
                     /locus_tag="ACHUT9_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFR1349483.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            complement(8111..10039)
                     /gene="thrS"
                     /locus_tag="ACHUT9_003070"
     CDS             complement(8111..10039)
                     /gene="thrS"
                     /locus_tag="ACHUT9_003070"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFR1349484.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            10564..12462
                     /gene="espL1"
                     /locus_tag="ACHUT9_003071"
                     /pseudo
     CDS             10564..12462
                     /gene="espL1"
                     /locus_tag="ACHUT9_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            12634..12741
                     /gene="yniD"
                     /locus_tag="ACHUT9_003072"
     CDS             12634..12741
                     /gene="yniD"
                     /locus_tag="ACHUT9_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFR1349485.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(12794..13552)
                     /locus_tag="ACHUT9_003073"
     CDS             complement(12794..13552)
                     /locus_tag="ACHUT9_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFR1349486.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT         3691 a         3362 c         3033 g         3589 t
ORIGIN      
        1 ggtaagaatc atgctttgat aaccaattga taactattat tatattaatc taacaccatt
       61 gacatagttg aggtttgaat gaacaaaaag ctgtatttat tcccagactc aaacattttt
      121 ctacaatgca aggacttgac gcaggtaaat ttttccgaga ttactgcttg tgatgaagta
      181 tgtatcatta ttacaaggcc cattcagcaa gagattgacc gccagaaagg gcaaggtaat
      241 tcccgcctat ccaaaaaagc aaggaaggct gctagcctat ttaatcaagt agttgattca
      301 actgacatga ctcttgttat tcgtgaacgc tcgccgcgag tttttcttac catggatcta
      361 agcctgaaac catgtgaaca gttatctgaa caattggatt atcaggaggc cgacgatcgt
      421 tttgttggta ttgcggcagg gtattgcgct gacgatccag attcggaggt ggcaatcatt
      481 accaatgata gcggtccacg tttttcagca ataaaacata atataatctg ctataaagtg
      541 ccttcttcat ggttgcttcc tgctgagcca gatgaaaagg ataaacaaat taaattgctg
      601 gaagctaagc tgaagcaatt cactcagtct atgcccgatt tcagcattaa agtagccgat
      661 gacgagcaat taaatattgt acttccttat tatacagctt tatctgaata tgaagttgat
      721 aatttgcttt gtcaactaac ggatgcattc ccattggtta caaactttga caatgaacaa
      781 actaccccac aatcactaga aaaaatcatc aagtcattta gtcaacagga gtatttccct
      841 gtatcagcag atgatatttc tctttataaa gaaaaacatt atccactttg gaaaaagcag
      901 tgtgaagaaa tactgaaaaa ctgtcatact catattaatc cgaaggcaat aaattacccg
      961 ctgtcgtttt tattacaaaa cacaggttat actatcgctg aacgtgctgt ggtaacattt
     1021 acagcaaaag gagattttcg tctgtgtgga tttaattggg agataatgga agagctgact
     1081 gaaagcaaaa ggattgacct accttcagcc cctaccgccc ccaaaggaaa atggcgtagt
     1141 gtatgtggcg taacagaaag aatgccttac gctgggggag ctttggaaac accgctatcc
     1201 agtataattt caaaagaaat tggtcctttg ttatctctta ctccaacaga aagggataaa
     1261 aacgattttt attataaaga aaagagaaat gagccagttc aagttgtatc gctggaatgc
     1321 gaagagtggc gtcatcaagt caaagaagaa atattcccgt tgttcgtata tacagccaac
     1381 aaaccaaaga aagtgaatgg tgcaattgag gtgagagttg atgcaaataa ccttaccaat
     1441 ccggtcatta aaacatttaa attaaaaata actatagaag aacatagtgc attttcaacc
     1501 ataacagaaa tggtgcagac atacattcaa gagcagaaga aaagacagaa aagttctatt
     1561 agcgcacaaa tcaaaacatg agggtgtact caaataatac aatgctatag taaatctata
     1621 agtgcataac tatttcctga tcttttccca aaatagatga caggataaaa aacattccaa
     1681 tcttttacag gctttggata aatcgtatca aagcctattt cttcaagagt taaatcaccg
     1741 cttttcgctc gaaacggact ggctgttgga gtatgagcca gaacaactta gaatagcccc
     1801 tgcactacgt taagggaggt cgctatgttc cgttgtccgc tttgtggcgc atctgcccgt
     1861 atccgcacca gtcgtccgga aaatgattca aacaccgtgc ggcaaaagta ttaccagtgt
     1921 aacaatctgg aatgcggcgt atgcttctca acactggaag ctttccataa attcacatca
     1981 aaacacgcct ccggcgttca ctcttcagaa gatatcccgt ggcatgatct gccagcttca
     2041 cacaggggaa acaatcagat gagcttgcct ttacctcaga attaacaggc agaattgccg
     2101 gagtaacaaa aaagcgatag attacgcgcg ggtgcctttc ggctgatggt cggagggaat
     2161 acccgaaggc cagatgtgga aaggccccgg aaaacatctc tgtttaaccg aggccctaac
     2221 cgcattacct tgacaagtga aaggttagcg cctctccgga aaaggagcaa gtgctatgtc
     2281 gcaaaaatcg cttacgacca tcacgttctg cgtgacggca atcctcatca tctggatgtt
     2341 gcacggttcg ctgtgtgaaa tacggatgag cttctgggga gcggagtttg cggcgttctt
     2401 acagtgtaag cagtaaggaa accgcgacgg gggagcaatc ccccgtcaat cggttgccag
     2461 ggtaaggccg gataaggcac cctatctcac agatatgaac aacaaacccg cagcgtaaaa
     2521 actgcgggtt ttctttttgg tgccctcacc gacttgaaac agtgataaga ctataagaca
     2581 aagcccgcaa tgtagcgggc ttactcccct caccctccac tgaatgtgga cacacagtgg
     2641 aacgaagcgg cataagatat tataaatcaa tcagttattg aaagaaaaaa ggccgcagag
     2701 cggccttttt agttagatca gattactcgt ctttgggcga agcgttttcg acccggcttt
     2761 ttaacttctg cccgggtctg aaggtcacca cgcgccgtgc tgtaatggga atatcctcgc
     2821 ccgttttcgg gttacgtccc gggcgttgat tcttatcacg cagatcgaag ttaccaaaac
     2881 cagagagttt cacctgttcg ccgttttcca gagcgcgacg gatctcttcg aaaaacagtt
     2941 caaccagttc tttggcatcc cgcttgctaa gcccaagctt atcaaacaga tattctgaca
     3001 tttcagcttt tgtaagcgcc ataggttcaa tccctcaatg atgcctggaa tcgctctttt
     3061 aatgcctcta cacatttggc gacggtagcg gcaatctcct cttcttcgag tgtacggctg
     3121 gtatcttgca ggatcaggct tatggcgagg ctcttatacc cctccgcaac acccttaccg
     3181 cggtacacgt caaataagtt tacgccaact acctgattta cgccaacttt cttacattcg
     3241 gataaaatat ccgctgcggg aacgttttct gcgaccacca ccgcgatgtc acgacggttc
     3301 gccgggaagc gagaaatctc gcgcgcctga ggcaccacgc ggtctgcgag cttgttccac
     3361 tccagttcga acaccagagt gcgaccgtta agatccagtt tacgttccag ttcaggatga
     3421 acaactccaa caaaaccaat acgttcccct ttcagataaa tcgctgcgga ttgccccgga
     3481 tgcagtgccg gattcgcttc tgcacggaac tcaacctcat tcagtttacc ggtcaggtcg
     3541 agaacggatt caagatcgcc tttcaaatca tagaaatcaa cggtctcttt tgccaggttc
     3601 cagtgctctt cgtaacggtt accgcaaatc acaccggcta acatcagatc ctgacgaatg
     3661 cccaacggtg cctgagtatc tggtacgaaa cgcagaccgc tttcgaaaat gcgcacacgg
     3721 ttctgctgac ggttctggtt gtacaccacg gttgccagca ggccggtcca cagagaaaga
     3781 cgcattgctg acatttcaac agagatcggg cttggcagca gtaaggcttc aacgcctgga
     3841 tggatcatct gctgcacttt cggatcaacg aagctgtagg tgatcacttc ctgatagcct
     3901 ttgtcgttga gcagcgtttt cacgcgcttg agcgacaggt cagcttcacg gtgagtaccc
     3961 ataatcaggc ttgcctgtac cggctcatcc gggatgttgt tgtagccgta aacacgcgcg
     4021 acttcttcaa ccagatcttc ttcaatctcc atatcgaaac gccagctcgg cgcaactgcc
     4081 tgccactcgt ctttgccttc ggtcacttcg cagccgagac gacgcagaat gtcagttacc
     4141 tgttcatcag caatatgatg gccgatcagg cgatccagtt tgctacgacg taaagtgatg
     4201 gttgcacgct tcggcagcgt tgcttcgttg gtgatatcaa ttaccggacc agcctcacca
     4261 ccgcagatgt cgatcagcag acgggtcgca cgttccatcg ctttgtgctg cagtgccgga
     4321 tcaacgccac gctcataacg gtgagacgca tcagtatgca ggccatgacg acgagcacga
     4381 ccggtgatag acagcgggct gaagaaagcg cattccagca agacgttttg cgtttcgtca
     4441 ttcacgccag agtgttcgcc accaaagatg ccgcccatcg ccagcgcctt gttgtggtcg
     4501 gcgatgacca gagtgtcagc attcagcttc gcttcagtgc catcaagcag caccagcgtt
     4561 tcgccctctt ttgccatccg caccacaatg ccgccttcaa tgcgatcttt atcgaaagcg
     4621 tgcatcggct ggcccagttc gagcagtaca tagttggtga cgtcaacaac tgcatcgata
     4681 gaacggatcc cgcaacgacg cagtttttct ttcatccaca gcggagttgg cgctttaacg
     4741 ttaatgcctt ttaccacacg gccaagatag cgcgggcagg cttcaggcgc ttcgactgta
     4801 atcggcaacg tgtcgtcgat ggtcgcacca accggaacga tttccggttc aaccagcggc
     4861 agctgattca gcacggcaac gtcacgcgca acaccaatga tacctaagca gtcggcacgg
     4921 tttggcgtta cgctgatttc gatggtgttg tcatcgagtt tcaggtattc gcggatgtca
     4981 gtgccaatcg gcgcatctgc aggcagttcg ataatgccgc tgtgatcgtc agaaattccc
     5041 agttcagaga aggagcacag catcccttca gacggttcgc cacgcagttt cgccgcttta
     5101 attttgaaat cacccggcag aacagcacca atggtagcta ccgccacacg caggccctga
     5161 cggcagtttg gcgcaccaca gacgatgtcc agcaggcgat cgccgccgac attcactttt
     5221 gtcacacgca gtttgtcagc gttcggatgc tgcgcacact caaccacttc accaacgacc
     5281 acgccgtgga agctaccggc aaccggttct acaccgtcaa cttccaggcc cgccatagtg
     5341 atttgattcg ccagcgcatc gctatcaatc gccgggttca cccattcgcg taaccacagt
     5401 tcactgaatt tcataatcta ttcctgcctt atttaaactg tttgaggaaa cgcagatcgt
     5461 tttcgaagaa tgaacgcagg tcggtgacgc cgtaacgcaa catagtcaga cgctccatcc
     5521 ccatcccgaa ggcgaaacca gagtaaactt ccgggtcgat gccaacgtta cgcagcacgt
     5581 tcggatgcac catcccgcaa cccagcactt ccagccattt accgttttta cccatgacgt
     5641 caacttctgc agaaggctcg gtgaacggga agtaggaagg acggaagcga atctgcaaat
     5701 cttcctcaaa gaagttacgc aggaaatcgt gcagagtgcc tttcaggttg gtaaagctga
     5761 tgttggtatc aacaatcaga ccttccatct gatggaacat cggcgtgtga gtctggtcgt
     5821 agtcgttacg ataaacgcgg ccaggcgcga tgatacgaat cggtggctgc tgggctttca
     5881 tggtgcggat ctgtacgcca gaggtctggg tacgcagcag gcgggtagtg tcaaaccaga
     5941 aagtgtcgtg gtcagcgcgc gccgggtggt gaccaggaat gttcagagca tcgaagttat
     6001 gataatcgtc ttcgatttcc ggcccggttg ccacggtaaa gccaagctca ccgaagaaac
     6061 tttcgatacg gtcgatggta cgggtaaccg gatgcagacc gccgttttca atgcgacgac
     6121 ctagcaggga gacatcaatc gtttccgccg ccagacgcgc attcagcgct gcgctttcca
     6181 gttccgcttt acgcgcattc agcgcctgct gaacctgctc tttcgcttcg ttgataaccg
     6241 caccagctgc cggacgctct tctggcggca gctcacgcag ggtcgtcatc tgaagggtta
     6301 agtgcccttt tttacccaaa tattcgacgc gcacattatc taacgcggca acatctgacg
     6361 cctggctaat ggccgccttc gcactggcaa ccagttctgc gagatgtgac atggttttcc
     6421 tcattgtgtc agtggtgaca ctggttcgtt ggacttagag cctatcccat caggctattt
     6481 tacttgccat tttggtcccg ggcagtgctc gaaattctca cgtacttaaa tacgctccgg
     6541 tttctccgcg ctggccgtgt ccagactggc tgcgacaatt acgcctgata gactggcttt
     6601 ttatttttca aaacgcgcat acaaaaaaag cctccactgg gaggctttca ggcgctgttt
     6661 tccgtttctc ttctcacgcg ctagcctcct ggattcaggt gctaaagtaa aaaaagaagc
     6721 ggaaaatagc agcattcatt gcttgcgtta ccttttggta ctcttcaaaa gacctttatt
     6781 gaaaaggcta cggcgataaa agtcaatgtt ttgatggcgt tgaaacgaaa agagggagac
     6841 tagctccctc tttcaactgg cttatgccag agctgctttc gctttttcaa ccagagcggt
     6901 gaacgctact ttgtcgaata ctgcgatatc agccaggatc ttacggtcga tttcaacaga
     6961 ggcttttttc aggccattga tgaatttgct gtaagaaata ccgttctgac gtgctgctgc
     7021 gttgatacgc gcaatccaca gttgacggaa ctgacgctta cgttgacgac ggtcacggta
     7081 agcatactga ccagctttga taacagcctg gaaggcaacg cggtatacgc gagaacgcgc
     7141 accgtagtag cctttagctt gtttcaaaat tttcttgtga cgtgcgcgtg caataacacc
     7201 acgttttacg cgagccatat gtgctctcct gtatctatat tctaattaaa aagttaaaaa
     7261 cgttaacggc ttatgcgtac ggcaggcacg cgattaccag gcccagatcg cctttggaaa
     7321 ccatggcttt cggacgcagg tgacgtttac gtttggtcgc ttttttggtc agaatgtgac
     7381 gcaggttagc gtgcttgtgc ttaaaaccac ctttaccggt ttttttgaag cgcttagcag
     7441 caccgcgtac ggtcttaatt tttggcattt taataacttc cacttcgcat tgttaataaa
     7501 cgaaacaaag gcgaacaaag cctgtgaagc ccgaaggctc cacagacagt gctacttgaa
     7561 ggccttactg tttcttctta ggagcgagca ccatgatcat ctggcggcct tcgatcttcg
     7621 ttgggaagga ttcgaccact gccagttctt gcaaatcgtc tttcacgcga ttaagcactt
     7681 ccataccgat ttgctggtgc gccatctcac gaccgcggaa acgcagcgtg attttggctt
     7741 tatcaccctc ttcgagaaag cgaatcaggc tgcggagttt tacctgatag tcgccttcat
     7801 ctgtaccagg acggaattta atttccttaa cctggataac tttttgcttt ttcttctgtt
     7861 ccttagaaga cttgctcttt tcatagagga atttgccgta atccattata cgacaaaccg
     7921 gcggctcggc gttagggctg atctcgacta agtctactcc ggcttcttct gctttctcca
     7981 gagcttctct cagactcaca ataccaagct gctcgccttc cagacctgtt aagcgaactt
     8041 cctgggcgcg aatttcgcca ttgatacggt tagggcgcgc cgtttgaact cgttttccgc
     8101 ctttaatacc ttattcctcc aattgtttaa gactgcggct gcgaatctct tgttgcagct
     8161 tctcgatcac ttcatttacg tccatgcttc ccaggtcttt accacggcgg gtgcgaacgg
     8221 caactttgcc tgattccacc tctttatcac cacagaccag catatatggg acgcgacgca
     8281 aagtgtgctc gcggatttta aagccaatct tctcatttct caagtctgct ttaacacgaa
     8341 tgcccgcatt tgatagtttt tgcgtcaatt cgttaacgta ttcagactgt gaatcggtaa
     8401 tattcatgat aacaacctga actggcgcaa gccaggtcgg gaagaaacca gcgaattctt
     8461 cggtcaggat accgatgaaa cgttccatcg accccagaat tgcgcggtga atcattaccg
     8521 gtaccttacg ctcgttgtct tcgcccacat aggaggcgct caaacgagac ggcaaggaga
     8581 agtccagctg tactgtaccg cactgccatg cacgatcgag gcagtcatac agggtaaatt
     8641 caattttcgg accgtagaaa gcgccttcac ccagttgata ttcaaacggg atgttgtttt
     8701 cttccagcgc aaccgccagg tccgcctcag cacgatccca catttcgtcg ctgccaatac
     8761 gtttttcagg acgagtggag agtttgacga cgatcttctc gaagccaaaa gtgctgtaca
     8821 tatcatagac taaacggata catccgttaa cttcatcgcg aatttgttct tcagtacaga
     8881 agatatgcgc gtcatcctgg gtaaatccac gcacgcgcat caggccatgc agcgaacctg
     8941 acggctcgtt acggtggcag ctaccaaact cggccatacg cagcggcaga tcgcgataag
     9001 acttcagccc ctggttgaaa atttgtacgt gacccgggca gttcatcggc ttaatgcagt
     9061 attcacggtt ctcagaagat gtggtgaaca ttgcatcttt gtagttgtcc cagtgaccgg
     9121 ttttttccca caggacacgg tccatcatga acggaccttt aacttcctga tactggtact
     9181 ctttcagttt agaacgaaca aacacttcca gttcacggaa gatggtccag ccgtcgttgt
     9241 gccagaatac catacccggc gcttcttcct gcatatggta caggtcgagc tgtttaccga
     9301 ttttacggtg gtcgcgtttc gcggcttctt ccaggcgctg caggtaagcg ttaagtgctt
     9361 ttttgtctgc ccacgccgta ccgtaaatac gttgcaacat tttgttgttg ctgtcgccac
     9421 gccagtaagc ccctgccgtt ttcattagtt tgaaatgatg gcagaaacgc atgttcggta
     9481 cgtgcggacc gcggcacata tcgacatatt cttcatggaa gtacagacct ggcttgtcat
     9541 catgggcgat gttttcgtca agaatggaga ctttgtagct ctccccacgg ttggcgaaag
     9601 tttcacgcgc ttcgtgccag ctgactttct tcttaatgac gtcgtagttt ttctcagcaa
     9661 gctcatgcat ccgcttctcg agtgcttcga catcttcctg ggttaacgtg cggtcaagat
     9721 caacgtcgta ataaaaaccg ttgtcaataa ccgggccgat tgccattttg gtatgcggcc
     9781 aaagttgttt aatcgcatgc cctaacaggt gcgcacagga gtgacgaatg atctccagac
     9841 cttcttcgtc tttggcggta atgatcgaca gttgtgcgtc gttttcaatc agatcgcaag
     9901 catcaaccag ttcgccatta acgcgccctg cgatacaggc tttcgccaga cctggaccaa
     9961 tgtccagcgc aacatccatg gggcttacag cgtggtcgta atggcgttgg ctgccatcag
    10021 gaagagttat aacaggcatt ttatatcctt aatttgcagt ggtgacccac acgaaagatc
    10081 acatacaaag aaaaatttgt ttattaacag ttcattgcga aaccatctag ccaacaaatg
    10141 ctaaattggt tcgcaatcag gtacacaaat gaaagacatt aacctctcac cttccacccg
    10201 gttgaacgat gttaacacta aaaaaaggga gattgtacct ttccgtttca cattgattcg
    10261 attcgcaatt cgtttgtagt gagtacacca gcaaaacgct taaagaacca ctgataacat
    10321 cacggtgcgt ctgaaaggtt tactgcgagc ggatttacag cagcataaac tgaatccagc
    10381 aaagttcgtt gcgtgatttg gcggtagtat tttaaacgct catgagatta atgacgaagt
    10441 ggtcatatca caatgataaa agtgacacaa ttcttatagc aatttttcgt gcacatttcg
    10501 ttctggcgat aataattaat catcatcctc aaaacctcca gacatatata aggcgaatag
    10561 attatgagtc aaaacgatat cattatcaga acccattata agtctcctca tagattgcac
    10621 atcgatagcg acataccaac gccttcatca gagcctatta atcaatttgc gcgccaactc
    10681 atcaccctac ttgatacctc tgacttaagt tcgatgctgt catactgtgt tactcaggaa
    10741 tttaccgcaa actgtcgaaa aatttcacaa aattgttatt ccactgccct ttttatcatt
    10801 aactttgcca cttcacccat ccatgcagaa aatatactca ttacattaca ctataaaaaa
    10861 gaaatcattc ccttatttct ggaaaccacg cctattaagg ctaaccattt gcgaagcata
    10921 ctggattata ttgaacagga acagttaact gccgaagatc gtaaccattg tatgaaactg
    10981 tctaaaaaaa tccatagaga aaaaactata caaccaacag taaatctcaa tggtagtgca
    11041 tttttttcgc aatctccttc tgacgctatt ttttgtcgcc atctgtcatt gcaatacgcc
    11101 cttgattcat tgagaaatgg aaaaggcaaa gtcaacctga ttaaacacta ctcctccgtt
    11161 gaatccatac agcagcatgt ccccttagtc cgggacgcgg agttcagagc attacttcgc
    11221 catcctcctg cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tttagatatt
    11281 ttcttttgtc gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt atatatagac
    11341 aatcatactt tgtcagtaag gctacgaata aagcagtcag tgtatggaca attaaattat
    11401 gttgtgtctg tttacgaccc gaacgatacc aacgttgccg tcagaggcac ccacagggca
    11461 gcacggggct ttctctcgct tgataagttc atcagttcag ctcccgatgc tcagacctgg
    11521 gctgataggt atgttcgcaa ctgtgcaatt gctattcttc ctctattacc tgagggagtt
    11581 ccagttgcta ttttagcggg tatcacgaca cgaatgccat ttgcccctat acatccatcg
    11641 gcaatgttgt taataatggc cacaggccag actcaatagc ttattacact attcaaacag
    11701 ttgcccatac tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa tagcgttggt
    11761 acacctgctt tatttctggc tatgatgaac ggacaaactg acaacgtaaa aatatttatg
    11821 caagaaattc agtcactggt atacaatcat atcattcatg aagataatct ggttaaatta
    11881 ctgcaaacta aaagtgctaa cgaaacacct ggactctata tctccatgtt gtatggattc
    11941 gatgaaataa tcgatatctt tctgaatgca ttaaccactc caatagcaca agaactttta
    12001 aacaaaaaaa tggtgatgag tattttagcc atgaaaatac atgatggtga gccaggatta
    12061 tacgccgcaa tggaaaataa tcaccctttg tgtgtcacac ggttcctctc taaaattaat
    12121 ggcatcgcct ttaaatacaa gttgagcaaa gctaacatca tggatttatt aaaaggagct
    12181 acagcacagg gaacccctgc attatacatc gccatgagca agggtaatga agacgtcgtg
    12241 ttatcttata tatcgacgct gggtgctttt gcaaaaaaac attcttttag tcaacatcag
    12301 ttatttacac tattggccgc taaaaatcat gacaacatgt cagctgttca tatagccatt
    12361 catcataagc attataaaac tgtagaaaca tattatgctg ctataaatgt catcagccaa
    12421 agcctgagtt ttagtgctga tgaaataaag acgtatttat aaccagctat ttacgatata
    12481 tatcgataca taattttatt tttttcataa aacgcagaaa cagtgaccat gtgcacaacg
    12541 ccacccgacg gctgtaataa caaaaaatcc cttgatgcct atcccttttg ttacactccg
    12601 ttatcacgca caagagatat gcaggacact ggtatgccga ctaaacgctt tgataaaaaa
    12661 cactggaaga tggtggtggt gctactggca atctgtggcg ctatgttgtt gctacgttgg
    12721 gcagcaatga tttggggctg agcagtgaca aacgaagaca gccagaccgc ataacacggt
    12781 ccggcagata attttacatg ctgtagccca gcgataaggt tgtccgacga tcggtatgtt
    12841 ccggtgcaga ctctggaggt tcagagttcc atgtgacgtt gtaagccacc ttcagtccaa
    12901 aatgttcatt aatagcaaca tttaatgcgc tttcggagtt cagcgttgta tcttccgcgc
    12961 caaagaccga aacaccctgc gtaaatttag cgttgtcagt caactgccat gcataagccc
    13021 cggaggcata gcccagcggc tgggtttcac tggcattgtc ggtatatttg tcgtaacgca
    13081 cacctgggcc gaattcaaag cggaaactgt gtaccgggcc attcaaaaac tggcgaccat
    13141 aacccgcggt caacacatcg cgctctcgat agccgttata acggtctgtc agccagcttg
    13201 cctgaccaaa taaatagtca taatcagtta aattaaaacg gctacgcccg cccgccgcat
    13261 atttttctga agaacgctca tcattagaag aagtattact ggcgttcccc cacagcgacc
    13321 aggcagtggt ttgtccatac caggtcatgg tggtgtcagc cgtaagtgag gagcttttcg
    13381 tattgcctga ttgtgcaaga tatcctgcgt tcagattacc ttcgaaaggt tttttagcgc
    13441 tggcaggatc gtccatgaca gtaaaaacgg aatcatcggc agctgcattc agtgacgcaa
    13501 acatgccccc cgccaacata acgatggcag gaactgtctt caaaagcttc atttatcaag
    13561 agtccgtaca acaaaaaaag agaccatcgc ggtcccggaa actttcttaa ggatcaaaga
    13621 ttagcgtccc tggaaaggta acgaattata aaaaggcgcg aataacttag caatg
//
LOCUS       DAZPEP010000112        13334 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZPEP010000112 DAZPEP010000000
VERSION     DAZPEP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.112"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..312)
                     /locus_tag="ACHUT9_003074"
     CDS             complement(<1..312)
                     /locus_tag="ACHUT9_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HFR1349487.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGSTTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LA"
     gene            complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUT9_003075"
     CDS             complement(331..2208)
                     /gene="bglF"
                     /locus_tag="ACHUT9_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFR1349488.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPTVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUT9_003076"
     CDS             complement(2342..3178)
                     /gene="bglG"
                     /locus_tag="ACHUT9_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFR1349489.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHUT9_003077"
     CDS             complement(3464..4189)
                     /gene="phoU"
                     /locus_tag="ACHUT9_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFR1349490.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHUT9_003078"
     CDS             complement(4204..4977)
                     /gene="pstB"
                     /locus_tag="ACHUT9_003078"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFR1349491.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHUT9_003079"
     CDS             complement(5068..5958)
                     /gene="pstA"
                     /locus_tag="ACHUT9_003079"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFR1349492.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHUT9_003080"
     CDS             complement(5958..6917)
                     /gene="pstC"
                     /locus_tag="ACHUT9_003080"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFR1349493.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHUT9_003081"
     CDS             complement(7004..8044)
                     /gene="pstS"
                     /locus_tag="ACHUT9_003081"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFR1349494.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUT9_003082"
     CDS             complement(8292..9365)
                     /gene="lpfD-O113"
                     /locus_tag="ACHUT9_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFR1349495.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUT9_003083"
     CDS             complement(9376..11898)
                     /gene="lpfC-O113"
                     /locus_tag="ACHUT9_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFR1349496.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSCVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUT9_003084"
     CDS             complement(11923..12654)
                     /gene="lpfB-O113"
                     /locus_tag="ACHUT9_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFR1349497.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUT9_003085"
     CDS             complement(12702..13274)
                     /gene="lpfA-O113"
                     /locus_tag="ACHUT9_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFR1349498.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSLVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT         3503 a         3399 c         3006 g         3426 t
ORIGIN      
        1 cgctaacccc gcttcattcg gttcggcttc gtcgccctga gggaaaattc gcgcccaggc
       61 aatggaaata cgcagacagg tgaagcccat ctcggcaaat aacgcgatat cttccgggta
      121 acggtgataa aaatcgatgg cgacatcttt gatattctct ttccccagga tgcgcggttc
      181 catttttccc ataacgccat gaggctgtaa atctgaggtc gtgctccctt tgccatcttc
      241 cagccaggca ccttccacct gattggcagc tgttgcgcca ccccaaagaa atgtttctgg
      301 aaatgctttc ataattaact ccttttatcg ttagcgaatg atggataaca gcggttcacc
      361 tgcgcttatc tgcgccgtgc cgtggggtaa tacgtctgta aaatcatcgc tattactgat
      421 taataccggc gtcgtcacat caaatccggc ctcgcgaata gcagggatat caaaagaaat
      481 cagccgatcg cctgtattga ccttgtcacc cacgttgacg tgagcggaaa agaatttgcc
      541 gtccagtttt acggtgtcga taccgacatg aatcaggatc tccacaccat catctgactc
      601 aatgccaatg gcgtgtaatg tggcgaacaa cgaagcaatt cgacccgcaa ccggagaacg
      661 cacttcacca accgtgggca gaatggcgat acctttaccc aacaggccac tggcaaacgt
      721 ggtatcagcg acgtgaatga gcggcacaat ctctcccgtc atcggtgaac agataccgcc
      781 ctgctcaggt ggtgtaataa cctctggtgt tttctcttgc ggggcaccct gcgctggctg
      841 acgtttagcg gtgatgaaat gaagcatcac cgtaccgaca aatgcgcaac cgatggcaat
      901 gacaccgcca ataacgctgg cccagacggt gaaatcaatt cccgttgacg ggatggtttg
      961 catgaaggtg aaaatacttg gcaaaccaaa ggagtacact ttcgtttgcg cgtagccaat
     1021 gatggtggcc cccaaagccc cactgataca ggcgataaca aaggggtact tacgcggcag
     1081 attaacgcca tataccgctg gttcggtgat accaaacagg cccgtcaacg ccgctgatcc
     1141 cgccaccact tttttctgcg catcgcgttc gcagaggaag acgccgagcg ccgccccgac
     1201 ctgcgccata atggcgggca ttaacagcgg gatcatggtg tcgtagccca gcacggtgaa
     1261 gttattgata cacagcggca ccaggcccca gtgcagtccg aacatgacga agatttgcca
     1321 gaagccgccc attaccgcgc ccgcaaatgc aggaaccgcc tgataaagcc agagataacc
     1381 ggcggcaatc agttcgctta tccaggttga tagcggcccc accagcagaa aggtgacggg
     1441 tgtgataacc atcagacata gcaatggtgt gaagaaattt ttgattgccg acggtaacca
     1501 cgcattaagt cggcgttcca gaatgctgca caaccaggca gaaaaaataa tgggaataac
     1561 cgatgacgag taattcaaca atgtgaccgg aatacccagg aaatccagcc ccagcgcatc
     1621 cgctttttgc ccgttctcga aagcagtcag aattaatgga tgcactaacg ctccaccaat
     1681 caccatggca gtaaatggat taccgccgaa gcgtttcccc gcggtgtatc ccaggattat
     1741 cgggaagaac caaaacaagg catcactggc gctgaataaa attaaataag taccactttg
     1801 ttcggtcgtc cactgaaaag tgagcgccag agccagcata cctttcagga tcccggttgc
     1861 cgccatcaaa ccgatcagag gcgtaaaaat acctgaaata acataaacaa agcggtttag
     1921 cagattacct ttctcatcat tttccggtgc ctgttgcgct ttttcgccaa ggcctgccac
     1981 gctgttgacc gccaggaaga catcggccac atggttacct atgaccacct gaaactggcc
     2041 accgctttcc accaccataa taataccggg ggtgtttttc agtacctcag cttgcgcttt
     2101 gctttcatcc tttaacttaa aacgtaatcg cgttgcgcaa tgcatcagac taacaatgtt
     2161 atctgcgccc ccgactcctg cgactatttt tctggctaac tccgtcataa cttgccctct
     2221 accgctttgc ggcaaaactc ccaaaaaaaa actgaaaaaa acggcctgac gtgaatcaag
     2281 caattttttt caggttttgc ccgcttagtg cggtaacaat cctttactca gtaataatat
     2341 ttcagtgttc tttgcgcacg cgctctatat ttatggctaa aaacataatc tctgcgggtg
     2401 aaattttacg ttgatactgc aaaccaataa aaatggcgat ccgttccgca cattgccatg
     2461 cttgcgggta attttgtttt actgcttgtt gtaatgattc atcactatcg ttaattgaag
     2521 catgttcaag aatacgccag gataaaaact tcagatgtgt aaccagtcgc tgataactca
     2581 agctttcttc ctggtaatta aggctgaact gaaattttat taattgcagc atttcgcgca
     2641 ttaactgcgt gacacctgca acatcctcca tatttccgct catttgggca ctgaccagat
     2701 gcatggcaat aaagcccact tcatctttcg gtaactgcac gcccaaccgt ttatcaataa
     2761 tggttaacgc ttcttcccct agctggaact ctttcgggta aagccgctgg atatcccaca
     2821 gcaacgggtt gggcagcaac acgttttgct gaaagcgttt aatcgcaaac tggcaatggt
     2881 cagttagcga gatataaata ctgtcctgta attttcccaa gcgctcctgc gctaaagaga
     2941 taatacgatc acaggttgcc atcacctcaa gaggaatatg acttaagagt tcgcttaatc
     3001 gcccgttcag ttcatgactg ctcaaggcat actctttttc tattccactt gagttaattc
     3061 tttcgccagc gcatttttga aagccaattc ctcgccccat gacgactttt tcccgctgtt
     3121 gatcatcaat aaccaccaca acattattgt tgagaatttt ggtgatttgc atgttcatag
     3181 caaggacctt ttttataaac aaaaaaaacc gacttcacca gtattctctg gttatgtcag
     3241 gttttgcctg cgaatgcagt aacaatccag tcatttatta atggttttta taacgaacat
     3301 ccaggttcgg aaattaattt aattgcgtgc agagaaaata gcaatgcgct attgataaaa
     3361 atatgaccat gctcgcagtt attaactttg tgtaatttta ggaatttata aagttatata
     3421 taacaaatcc caataattaa gttattggga tttgtctggt gaattatttg tcgctatctt
     3481 ttcccgccag cagtttatcc agctcatcac cgcctacatg acggaaatcc tgccccttca
     3541 cgtagtagaa gataaactca caaatattct ggcagcggtc gccaatacgt tcgatagaac
     3601 gcgcgcagaa cagcgcagta agtacgctcg gaatggtacg cgaatcttcc atcatgtagg
     3661 tcatcagttg acgaacaata ccttcgtatt cctgatcgac ttttttatct tcacgataaa
     3721 tacgtaccgc ttcgtcaatg tccatccgcg cgaacgcgtc cagcacgtcg tgcagcatct
     3781 ggatggtatg acggcccagc gactccagac ttaccagcaa cggctgatgc tgctgggaga
     3841 atttctccag cgcagtacgg cagattttgt ccgccacgtc gccaatacgc tccagctcgg
     3901 caatggtttt actgatcacc ataaccagtc gcaggtcgct cgccgtcggc tgacgtttgg
     3961 cgataatgcg cacgcaggct tcatcgatcg ccacttccat catgttgacg ttcttgtcgc
     4021 cttcgatcac gcgcttcgcc agatcgctgt cctggttatg catcgcggtg attgcatcag
     4081 aaagctgctg ctccaccatg ccgcccatgg tcatcacctg cgtgcggata ctttccagtt
     4141 cggcgttgaa ctggccggaa atatgtttat taagattgag actgtccata acgcactcct
     4201 gaatcaaccg taacgaccgg tgatgtagtc ttctgtttgt ttcttcgctg gcttggtgaa
     4261 cagatcgtcc gtgttgctga actcaatcaa ttcgcccagg tacataaacg ccgtgtggtc
     4321 ggaacaacgc gcagcctgct gcatgttgtg ggtaacgatc accacggtgt aatcctgctt
     4381 cagttcggtg atcagctctt caatacgccc ggtagagatg gggtcgagcg ccgaacacgg
     4441 ttcgtcgagc agcagcactt ccgggcgaat ggcgatacca cgcgcaatac acagacgctg
     4501 ttgctgacca ccagagagag agtaaccgct ctggtgcaat ttatctttgg tttcgttcca
     4561 caatgcggct ttggtcaatg cccactgcac gcgctcgtcc atgtcggcac gggagagctt
     4621 ctcaaacaga cgaacaccaa aagcgatgtt gtcgtagatg gacatcggga acggcgtcgg
     4681 tttctggaac accatgccca ctttcgcacg cagcagtgcg atatcctgag agttggtcag
     4741 gatgttgtcg ccatcaagca gaatttcacc ttctgcgcgc tgctccggat acagttcaaa
     4801 cattttgttg aaggtacgca gcagcgtcga tttaccgcag ccggacggcc cgataaacgc
     4861 cgttacctgg tttttagcga tatccaggtt gatgtttttc agggcatgga atttgccgta
     4921 gtagaagttc aaattacgaa cctgaatttt actcggggca gtttcaacca tactcattgc
     4981 aatcttttcc tcatcgtacc tgatgctttt cgctaatcag gcttaccgta ggccgggtaa
     5041 ggcgttcacg ccgcatccgg caaagtgtta accgtgttta ttcttcgcaa aaacaacgcg
     5101 cgccagaatg ttcagcagca ggacgcacag ggtaatgatc aataccccgg cccaggccag
     5161 ttgttgccat tccgcaaacg ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc
     5221 gatcggctgc atcatgtccg tgctccagaa ctggttggag agcgcggtaa acagcagcgg
     5281 cgcggtttca ccggcaatac gggcaatcgc cagcaggata ccggtcataa tcccggacac
     5341 cgacgctttc agcgtaatcg cggagatcat cttccacttc ggtgtaccca gcgcataagc
     5401 cgcttcacgc aggctgtccg gcaccagttt cagcatgttc tcggtggtgc ggataacgat
     5461 cggcacctgc aacagcgcca gcgcaattac gcccgcccag ccggagaagt gctccatctg
     5521 tgccaccacg atggtgtaga caaacagacc aaccacaatc gacggcgcgg agagcagaat
     5581 gtcgttaatg aagcgaatca cttctgccag ccaggattta cgaccatatt ccgccagata
     5641 aatccccgcc ataatgccca gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc
     5701 gctacccgcc agggcgttcg ccagaccacc accttccgta ttgggcggcg gcgtcatttc
     5761 agtgaacagc gccagcgaca taccgtcgat accgcgagtg atggtggaca ttaaaatcca
     5821 gatcagccag aacaggccga aggccatcgt cgccatcgag agcgtcagcg caatacggtt
     5881 tttgaggcgg cgacgcgcct gcattttgcg gcgagattca gccagcgccg cagtggtttg
     5941 catttcaacc atagccatta gcgtgccccc tcattcttag ccaggcgcat aatcataaac
     6001 ttcgatgcgg cgaggacgat gaaggtaatc acaaacagga tcaggcccag ttccatcagt
     6061 gcggcaacgt gcagaccgga ttccgcttcc gcaaattcgt tcgccaacgc agaggtgata
     6121 ctattgcccg gcatgtacag cgaggcgctg tcgagctggt aggtgttacc gatgataaag
     6181 gtcaccgcca tggtttcacc gagcgcgcgg cccagcccca gcatgatgcc gccgataaca
     6241 ccatttttgg tgaacggaag aacgatacgc cagataactt cccaggtggt gcagccaata
     6301 ccgtaggccg actctttcat catcaccggg gtttgttcga acacatcacg cattaccgcc
     6361 gcaatgtacg gaataatcat gatggcgagg atcacgcctg ccgcgaggat accgatacca
     6421 aatgcggggc cagagaacag cgcgccaaca atcgggatat tcgacatgat attgccgacc
     6481 ggctcctgaa agtaaacggc gaacagcggc gcaaagataa acaggcccca catgccgtaa
     6541 acgatacttg gaatggctgc cagcagctca atggcgatac ccagcgggcg tttcagccag
     6601 ccaggcgcaa gctcagtcag gaacagggcg ataccgaaac tcaccgggac ggcgatcagc
     6661 agcgcgataa acgaagtcac caacgtaccg tagatcggca ccagcgcccc gtagatatcg
     6721 ttcggtgcat cccactcttt ggtccatagg aaagccagac caaatttctg aatgctcggc
     6781 caggaggaga tgatcagaga gacaataatg ccacccaaca tcaatagcac aatcagcgcc
     6841 gccagtttta ccagcacgct gaaaattatg tcgccctttt tacccggtgg gttaaaagca
     6901 ggcttggttg cagccataag ttactcttca gttaaacgcg tttacaaagt cattttgtaa
     6961 tgccggatgc ggcgtaaaac accgtacccg gcctagagtt ttattagtac agcggcttac
     7021 cgctactgtc tttaatattg gtcttccacg cagcgcgaac ctgttcaact acactatccg
     7081 gcaggctggc gtaatccagg tcgttcgcct gtttagcccc ggttttgtac gcccagtcga
     7141 agaatttcag cacttctgtg ccttgttctg gtttcttctg atctttgtgg atcagaatga
     7201 acgtggtaga ggtaataggc catgcatctt cgcctttctg gttggtcaga tcctgagcga
     7261 aggttttgct ccagtctgca ccttttgctg cattagcgaa gttttcttcg gtcggactaa
     7321 ccggtttacc atcagcggag atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag
     7381 catattcaac ataaccaatt gcacccggca gacgctgaac gaacgcggcg ataccgtcgt
     7441 tacctttacc gcccagaccg atcggccatt ttacggtaga gccagtacca acgttgtttt
     7501 tccactcttc gttcactttc gccaggtagc tggtgaagac gaaggaagtc ccggagccat
     7561 ctgcgcggcg tactacagca atgttttgtg aaggcagttt cagacccgga ttcagtttgg
     7621 cgatggtttc atcatcccac ttcttgattt tgcccaggta gatgtcgccg agggttttac
     7681 catccagcac cagttcgcca gacttcagcc ctggaatgtt aaccgccagc accacgccgc
     7741 caatcacggt cgggaactgg aacagacctt cctgagccag tttttcgtca gacagcggcg
     7801 cgtcagaggc accaaaatca acggtattag cgataatctg ttttacgcca ccggaagaac
     7861 cgataccctg gtagttaact ttattaccga tttctttctg gtaagtgtca gcccatttgg
     7921 catacaccgg cgcagggaag gttgcacctg cacctgtcag gcttgcttct gcaaacacag
     7981 agaaagcgct catcgataag gtcgcggcga caacagttgc gacggtggta cgcataactt
     8041 tcataatgtc tcctgggagg attcataaag cattgtttgt tggctacgag aagcaaaata
     8101 ggacaaacag gtgacagtta tatgtaagga atatgacatt tttatgacag aaaggaatta
     8161 acaaataact tactgatttt aaatgtgaag atgaaaatcc cacatgtcat ctttttgtca
     8221 tgatgcattt aaaagtgata aagaaatggg tgagatgaca acgatgtgat gaacaatcgt
     8281 tgtcagacct attagcgcac aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc
     8341 ggggctgggc gagctccagt aaggctcgca ggagcagctg aaaaagttac actacctgat
     8401 ttttcaccat acactgttga tttacccatg tctgttggca actcaccacc gttcactttg
     8461 atttccttat tatttttgtc gtaaattttt atccccacgt tagagtttga agttacgacc
     8521 attgaattgt ccgtattggg ttcggtagca taaaaagacg tactgatatc atcggtattc
     8581 agtatcccca ggttttcaca ctcaatagtg actgttttag taattttatg tgttgttacc
     8641 gcacttccaa caacagatga aaaatctgca gctggtattt ctttaaaatc aaaataaact
     8701 ggctgtaaat tatttatttc gcaactttgc ggagctgtaa tatcgccccg aatataaaca
     8761 tcagccatcg gctgtgagta atccaccggg gtgtcacggc taattgttcc atacaatgtc
     8821 actatctttg tttcaggaat aatcaatttt cctgcaaatg tcttcttgat ataaaaatat
     8881 actatcgctt tacccccact ggatatactt aaaggttcaa tgcggttgca attgtacttt
     8941 gtattaccag acgggttgtt agcatgatat tcaaaaggtg cattcacata cccaacccca
     9001 agaatatcta tagcaatccc aacatcaagg ttcggattaa gatcataata attaatatta
     9061 gagtgaaccc cagcataagt taatgcctgg tttatttttg acgtataata gatatagggg
     9121 aattctccta cattaccagt atcacaatga cagcgtccag gatatgagga tccattagtt
     9181 acagtggtat taaatgtcgt tccaacctta ttttcgttcg cggtaataag atatcccgag
     9241 aaatcaagcg tacggtgaaa tacgccatta tcaggggtac agataccttt aggagctgca
     9301 agtgctacac tacttaacga gagcataaac acagcaaagc accactgttt gatatacttg
     9361 ttcataaaaa tccttttaat gacatgtcac accctgttca attaatccgc tctcactttc
     9421 tggtgtcgaa agtgaataat tgaagatgca ttttgttgag gcgttttctc cccaggataa
     9481 cgtaacctgt cctttttctg gtagaccaga aagataaagt tcgccacggt caccgacaat
     9541 tcctgaatct gccaaacttg catcttgtat cgcagcaatg gctccaaaag gtaccggttt
     9601 accatttgca tttaacactc tgaagagaac gcgataaccc acatgagtca caaactccgc
     9661 tttaaccact gcaccacgcg tgggaaccat cgtaacgacg gtattatcaa gttctatatt
     9721 cgacttcgag aaatagttat cgctgattgc tacccgattt tcatcataag ggtttagctg
     9781 tgtcttgacc gtatagccgc gccagtccgt cgcagcaccg cgcatattat ctatttctaa
     9841 cccggcggcc ccaggtgctt taacaagagc aatagtttca cctaactcct gacttaacgt
     9901 taccccttcg ctgtgtgcca gaattccccc tctgatacca taattcagcc tctgggaatt
     9961 ttggctgtag ttatagctgc cgttcaccgt gccataagag ccatcatagg tcagggacat
    10021 attaccgcca acctcatcat catgactgtt ataggactgc attaaattcc atgaaaggtt
    10081 atttctttcc agcgccgtac cgctgagtcc gagttgatgt gagctaccgg aattatcgtt
    10141 ttgcgaaaca gaataggtgg cgtaactatt accccaccag cccaataaag gaacagataa
    10201 agagagcatg tagatctgct cactggtgtt atcactggca tggttgttcc ccgtcccctg
    10261 tctggcagta ttggaataac tccaggagac gttccacgag agacgcttcc agctactgct
    10321 gtagccaaat tgcattaatc gttcgacatc atcggtatgc cagtattgtt gccggcttaa
    10381 tgtcaggtat aaattgccat aatctctccc caacgactgc gttagcgtcc cctccacgca
    10441 actacgtcgg ttacctgttt cccaaaagtc ttcagggctg ttgcgccgcg atgcccactc
    10501 attgagggta tagtagccct cggtggagta acgatagcca accaagcgca agctagtgtc
    10561 tgtgtcgtca aatcgttttg aatagagaaa gcgatatgac tgacccgttt ctgtatcatc
    10621 atggctaaaa ttcgcacgag catgtgtcac atcgaaagaa attgcgccca aagcgccaag
    10681 atctttaccg acacccagag ctgcagaacc ataattttca gcagcctgca tacctccata
    10741 taaagtgatc ccgtgggaaa aaccatgaag tatttgcgcc tgaaggacgt ccggtgtaaa
    10801 tccgtcttca tcgcgcaatt cacccacgct gacatcaaca tctgtctgac cttcacgttt
    10861 aagaatcgct aatgaagcat aaggttgggt gaatcgttgt tcgctaccgt cttcttcttt
    10921 gatggttacg tccaaatcgc cacctgtact ggcggtgttg agatcggtga tctcaaaagc
    10981 accttgtgga acggcagact gataaatcac gtagccattc tgccgaatga tgacggtggc
    11041 gttggacttt gcaatcccac gaaccacagg agcaaagcca ttctggctgg cgggtagcat
    11101 atcgttatca gtatacaaac gcgcgccgcg aatttgcgta ctgtcgaaaa tatcgctcgc
    11161 cgtccacgta tcaccaatca taatctggct tcttaacgcg gcaatatcgc gctgtatcca
    11221 ggtactaatg gagttgtatt ccgcacggcc atcgctggtg tttaaagtac tgtaattacg
    11281 tagccgccac ggtccaatat tcagtccatt gcgcagattg agataactgg tatctgtctc
    11341 ttgtgaacca taatctgaac gtagtgtatg tgaaccagaa aaatcgtagt tgatggtgag
    11401 cgcgttaatc ccctcatccc aacgcgtggg gctgatgtaa tcataagcca tcgcgtccat
    11461 gtagatttgg ggaatggata tattcagctg caatttatca ctgtcaaact gccaggatga
    11521 gccttcaata cgtggagcag gatcgaggca aaccgtctca tccacatcat tgaacgattt
    11581 gagcgcatca acttttatgc caaaggagga cagtgttttt ttaatggcaa taaagcaagc
    11641 gtgcagatca gcatctggat tatcctgaac aaatgtcaca tcctgacgtt caaacatttt
    11701 accgttgata aaaatatcaa cgctataaac acctgggggc atcaggtttg cccgattaaa
    11761 ctggcgaata tcgactccgg attgatggtc gatttccaga agcgccggat tgaatacact
    11821 gacctcggcc atcaggtttt gcgagaaaat cagacacgtc cctgccgtga ggccaaccac
    11881 tattctggtc gtcatcataa tacgcctatc cttatccctt gcttacagcg tcatttttat
    11941 cgggtcactg actgcgccgt aatcattaat aaatcgccag gtcatttggc ctcccgcaga
    12001 attagcaggt aaaggtaacg tgagcgtttc aaaaggacgg atcatattga ggggcacatc
    12061 atggccatca acgatgacat ccatcacact gatatgaatt gggttgggat tctctgctat
    12121 cagatttcgc ccttcccggc gccatttcac tttcggcgca gcatcttcgg gaataaggcg
    12181 gatatttgct ggtcgccaga acagcttgat acgcgttttt actgctattt ccagcgagtt
    12241 cgtcgctttg ttgctcgctg atggcggaat atttttaata ttcagccaga agactgactc
    12301 gcgatcatca ggcaatgaca cacctttggt gtgaataatg cgtaatattt tttcttgcga
    12361 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
    12421 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
    12481 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
    12541 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
    12601 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
    12661 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
    12721 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
    12781 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
    12841 ttataaagtt taaccagcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
    12901 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
    12961 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
    13021 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
    13081 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
    13141 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
    13201 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
    13261 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
    13321 atattaataa gtgt
//
LOCUS       DAZPEP010000113        13261 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZPEP010000113 DAZPEP010000000
VERSION     DAZPEP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.113"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="ACHUT9_003086"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="ACHUT9_003086"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1349499.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUT9_003087"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="ACHUT9_003087"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFR1349500.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUT9_003088"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="ACHUT9_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFR1349501.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUT9_003089"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="ACHUT9_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFR1349502.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="ACHUT9_003090"
     CDS             complement(4036..5568)
                     /locus_tag="ACHUT9_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFR1349503.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUT9_003091"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="ACHUT9_003091"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFR1349504.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUT9_003092"
     CDS             7206..8276
                     /gene="dadX"
                     /locus_tag="ACHUT9_003092"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFR1349505.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALVLRVPVVTV"
     gene            complement(8663..10399)
                     /locus_tag="ACHUT9_003093"
     CDS             complement(8663..10399)
                     /locus_tag="ACHUT9_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFR1349506.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUT9_003094"
     CDS             complement(10494..11408)
                     /gene="ldcA"
                     /locus_tag="ACHUT9_003094"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFR1349507.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUT9_003095"
     CDS             11508..12119
                     /gene="emtA"
                     /locus_tag="ACHUT9_003095"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFR1349508.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12121..12855)
                     /locus_tag="ACHUT9_003096"
     CDS             complement(12121..12855)
                     /locus_tag="ACHUT9_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFR1349509.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13056..>13261
                     /locus_tag="ACHUT9_003097"
     CDS             13056..>13261
                     /locus_tag="ACHUT9_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFR1349510.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVV"
BASE COUNT         3367 a         3372 c         3487 g         3035 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 accgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctgggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgagg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatccggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggtacgc
     8161 cggttgagct gtggggcaag gagatcaaaa ttgatgatgt cgccgccgct gccggaacgg
     8221 tgggctatga gttgatgtgc gcgctggtgc tacgcgtccc ggttgtgacg gtgtaacttg
     8281 ttgtaagccg gatcggaggc aacgtcttct gggtgcaaaa aaaatcatcc atccggctgg
     8341 tcagcaactg tagttgttaa tgtgacagag ccattgccca tgatagtgtc cattaaaagg
     8401 atggacacta tttccccgga acctgaactc accgcacagg cgttctacat aaaacgctta
     8461 cgcttcattg ttgactcgaa ctcgacttca gataaatcac gctttaccct tgatggagcc
     8521 tgtacataga tttgtgtaat tgcctgattt tgatatgttc aattcaacat caaatgaagg
     8581 ttaaattatg gacgacaaac aattgcaggc tcaggctgcg ttcagcaaag catcgcaacc
     8641 ggcgatagat gcttcattaa atttaagatt cagcttcctc ttcagccacc cgtacgccaa
     8701 tcttcaacac ttcattatct tctttctcgg ccaccgtcca gatcatcccg gcaaactcta
     8761 cctggtcacc gacaaccggt gccgcgccta acaactgctg gacaatttca cccagcgttt
     8821 gctgcttatc acgatattct cgcccgtctt ccaggccata tatcagcgca acatcagcat
     8881 atttggcgct ggcttcgaga atgaagtcac caaagaagcg ttgatccagc gcgaccggtg
     8941 gcgactggct gaacagttta ccgagcgccg ggagatcgcg ttcccgacca attacacaca
     9001 acacatcgcc ttcgcgcagt cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa
     9061 atagtgccgc aatacgcgtc tcttttggca tatgcagatc gcgcagtgcc gcacctacac
     9121 accatttatc ggcactcaat tgataaacaa actgctccca cggattttcc ggatgaatat
     9181 ccagaccaac gcgtgacact ggacgtccga cgggcggaac caccactttg gcttttttcg
     9241 ccgcccacga gagtgatgtt ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga
     9301 cattaaagaa cagacgtgca ttctccagcc ccgccatcat cgggaacacg gccaggatga
     9361 ttggcaccgc gccgcgtaat cctacccagc tgataaacac gcgctcacgc agattgaaac
     9421 cgcggaaggg gagcaatcca gcaaataccg aaaggggacg ggcgaagaat atcatccatg
     9481 ccgacaaaat gagcgccgga atggcaatag gcagcagatc gcttgggtta accaatagcc
     9541 ccagcaccag gaacatggcg atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga
     9601 tgccgtagcg gttgcgaatc ggccgattac ccagcagaaa accgcacaga taaacagcca
     9661 gaataccgct gccttccagc gccgtggtta aagcaaaaat taaaataccg ccgcttaatg
     9721 ccagcaatgg atataatccg gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat
     9781 aaccaccgcc aagtccgatg acaatcccga ggccaaattg ctgcagaata tcgaccacga
     9841 acatccaact gacactgctt tcatgttgct ggatcatcgc aatcagggta atcgtcagaa
     9901 agaccgccat tggatcatta ctgccggatt caatttccag cgtcgagcca acacgttcgt
     9961 taagcccctt accacccagc agagaaaaga ccgctgcagc atcggtagag ccgacgatag
    10021 cgccgattaa taagccttca atcaaatcaa gattaaacag ccacgccgcc atcatgccgg
    10081 ttaaaccaga ggtgataagc acgcccagcg tcgccagcga cagtgccggt cctaacgcca
    10141 cacgaaagga actggcctga gtgcgcatcc cgccgtcgag caaaataatc gccagtgcca
    10201 ggttacttac catgtaggcg aaggggtaat tatcaaacgg gataccgccg acgccatcga
    10261 ctcctgccag catgccgatc gccagaaaaa taaccagaat aggaatgcca agacgggaag
    10321 aaaatgaact aagtaatata ctgctggtga caaggatgga tcctaagatg aaaaggctaa
    10381 ttattgttgt ggcatccaac ggtcggttac tcctgattac gctgtctctt atataaaccc
    10441 taccatatta gcggcagaga cagcgtttta cttagccctg aagcgtgatt tttttacatt
    10501 ttaagaacag gatgaccgct aatcgtcaac tgagtgcctt cccgggtgtt attcagaatg
    10561 gcatgtgcgc ccaacggcag cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta
    10621 atgagtggaa tcgacaggcg ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct
    10681 gcgtcataat cattgggcgt gctgccgcta aagctaccga gaatgatcgc cttctgacgt
    10741 ggcaaaattc ccgcatgata gagctgcaac agcatacgtt cgtcccggaa agggtgctcg
    10801 ttaatatctt ccagcaccag aataccgttc tcaatttttg gcatccacgg tgtaccgatc
    10861 agtgaaatca gcatcgcaag attgcctccc cacaacgtgc cttcggcccg acatgtcggg
    10921 ccttcccctt gccattcaat ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc
    10981 tcggtaaagg cgttcagttc atccgcgcca aagtttgcta ccagcatggg gccgctaaag
    11041 gtgatgacat tgccatgcgc cagaagaccg cactgaatgg cggtaaaatc gctatgcccg
    11101 caaatgagca acgggtcatg ttgttggcga gccaccagtg cctgccagtc aatatctgcc
    11161 agtaaacgac tggcaccgta accgccgcgt acagccagca cgatggtgtt gggggtggtc
    11221 agtctggcaa gggaattgag atcttccagg cgctccgttt ccgtacccgc aaaacgctca
    11281 caacgacggg caataacctc gacgttattg acctgatgcc cctcgtcggt aaggcgctgg
    11341 ataccacgca gcgcggcgtg ctgtttaatg cagtaacccg atggggcaat taagtgaaac
    11401 agagacatgg caattccttg ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg
    11461 tgccgttgtc acctcaacgg cgattccagg ctataaggat agaagaagtg aaattgagat
    11521 ggtttgcctt tttgattgtg ttattagcgg gttgttcatc aaagcatgac tatacgaacc
    11581 cgccgtggaa cgcgaaagtt ccggtgcaac gtgcgatgca gtggatgcca ataagccaga
    11641 aagccggtgc agcctggggc gtcgatccac aattgatcac ggcgattatc gctatcgaat
    11701 cgggtggtaa tccgaatgcg gtgagtaaat cgaatgccat tggtttgatg cagttaaaag
    11761 cttcaacctc cggacgtgat gtttatcgcc gtatgggctg gagtggtgag ccgacgacca
    11821 gcgagctgaa aaatccggag cgtaatattt caatgggggc ggcttacctg aatattctgg
    11881 aaaccggccc gctggcaggc attgaagatc cgaaggtact gcaatatgcg ctggtggtgt
    11941 catacgctaa cggggcaggt gcgctgctac ggactttctc gtcagatcgg aaaaaggcga
    12001 tcagcaaaat caacgatttg gatgctgacg agttcctcga acacgtagcg cgaaatcacc
    12061 ctgcgccgca ggctccgcgc tatatctaca aacttgagca ggcactggac gcgatgtaaa
    12121 tcagtcgcgc actttgtccg ctttttcccg ggcttctcgc tcgagagaga aaataatccg
    12181 ctgtaattgc cgctccaccg tcgggctgac gttaagaaaa cggaagctca gacggggagt
    12241 ggtgatggtt tcattcttgc catcaatcac tttgcgctcg ctgatagaga ttaactgggc
    12301 gtcaaagtga aaaacacccc attgccccat gttgacttca atctgagcga agcgcatgcc
    12361 ttcttgtaat tcggcaggct ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa
    12421 atcatacagg cggaaacgta acgtactgtt atccgccagt ttggtctggc aaaaataagg
    12481 cggatggagt ggggcggaga tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg
    12541 aggcggtacg gtgataaatg ccggaagctg caagtattca ctctgctgta gttgttcaac
    12601 agtaaactcg actttcgcac cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag
    12661 cacagcgatg ttgtcttcgg cttgactgcc gaaatccagc accagtttat ccggggttat
    12721 tgccagtatt ttgctgatca gctgcccgcc attccaactg agacgcaaag gaattgcggc
    12781 tttgtgcaaa tcgcgtaaca cgcccaggac ggctaacgga ttttgtttca ggaactgctc
    12841 atggtaatga ctcacgcgga aaaactcctg actgacaaac tgtctttggt ttatcggtca
    12901 cagttaacaa aacttaatac aaatgcgtga atatttttta catgttgttc ttaaatcagc
    12961 cgacatacgc ccagcatcga tcccgccctt gcctatactt agagcgttga cgtaagcatt
    13021 tcttgcgtcc gattcatcga aacgagggcc tgaacatggg aattattgct tggattattt
    13081 ttggcctgat agccggcatt atcgccaagc taatcatgcc ggggcgtgat ggtggtggat
    13141 ttttcctgac ctgtattctc gggatagtcg gtgcggtggt cggcggctgg ctggcgacca
    13201 tgtttggcat tggcggctcc atcagcggtt ttaatttgca cagcttcctg gtggcggtgg
    13261 t
//
LOCUS       DAZPEP010000114        13081 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZPEP010000114 DAZPEP010000000
VERSION     DAZPEP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13081
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.114"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            109..408
                     /locus_tag="ACHUT9_003098"
     CDS             109..408
                     /locus_tag="ACHUT9_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349511.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(455..1342)
                     /gene="rarD"
                     /locus_tag="ACHUT9_003099"
     CDS             complement(455..1342)
                     /gene="rarD"
                     /locus_tag="ACHUT9_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFR1349512.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1394..1861)
                     /gene="yigI"
                     /locus_tag="ACHUT9_003100"
     CDS             complement(1394..1861)
                     /gene="yigI"
                     /locus_tag="ACHUT9_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFR1349513.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2026..2895
                     /gene="pldA"
                     /locus_tag="ACHUT9_003101"
     CDS             2026..2895
                     /gene="pldA"
                     /locus_tag="ACHUT9_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFR1349514.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3028..4857
                     /gene="recQ"
                     /locus_tag="ACHUT9_003102"
     CDS             3028..4857
                     /gene="recQ"
                     /locus_tag="ACHUT9_003102"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFR1349515.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4921..5538
                     /gene="rhtC"
                     /locus_tag="ACHUT9_003103"
     CDS             4921..5538
                     /gene="rhtC"
                     /locus_tag="ACHUT9_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFR1349516.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRTRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5600..6220)
                     /gene="rhtB"
                     /locus_tag="ACHUT9_003104"
     CDS             complement(5600..6220)
                     /gene="rhtB"
                     /locus_tag="ACHUT9_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFR1349517.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6331..7353
                     /gene="pldB"
                     /locus_tag="ACHUT9_003105"
     CDS             6331..7353
                     /gene="pldB"
                     /locus_tag="ACHUT9_003105"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFR1349518.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7361..8161
                     /gene="yigL"
                     /locus_tag="ACHUT9_003106"
     CDS             7361..8161
                     /gene="yigL"
                     /locus_tag="ACHUT9_003106"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFR1349519.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8237..9136
                     /gene="bioP"
                     /locus_tag="ACHUT9_003107"
     CDS             8237..9136
                     /gene="bioP"
                     /locus_tag="ACHUT9_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFR1349520.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9024..9977)
                     /gene="metR"
                     /locus_tag="ACHUT9_003108"
     CDS             complement(9024..9977)
                     /gene="metR"
                     /locus_tag="ACHUT9_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFR1349521.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10213..12474
                     /gene="metE"
                     /locus_tag="ACHUT9_003109"
     CDS             10213..12474
                     /gene="metE"
                     /locus_tag="ACHUT9_003109"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFR1349522.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12596..12718)
                     /locus_tag="ACHUT9_003110"
     CDS             complement(12596..12718)
                     /locus_tag="ACHUT9_003110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349523.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12759..13004)
                     /locus_tag="ACHUT9_003111"
     CDS             complement(12759..13004)
                     /locus_tag="ACHUT9_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR1349524.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3096 a         3251 c         3487 g         3247 t
ORIGIN      
        1 gtagtaattt agagtgtgta agtttttatt gatggggtat attttaatta aatataattg
       61 aaatgtattt ttattctata aacaatatct tgggtgaatg gattctatat gggaaatggt
      121 aacttttggc agttgttctt gggtaaagaa ccttacagca aaaaatctga ttttgttaag
      181 gcatggttat tgtggtgggc tattaccatc cttgttctat ttattttaag cctgactacg
      241 acattaaatt taagaagtat aggtataggg ttaagttttg ggttttgtca aatccttggt
      301 agatatacga tgtctctagg atggagtaag tactggggag ttttgggggt atttcctgta
      361 acaaatattg ttctttttct tatcttaatt tttttcaaaa aaagataaat aagtcagtgg
      421 gtaggctctt taaacggcct acccatgtta attcttaact ttttctacgc tgcgtataaa
      481 tcgcatccat cacaaaaatt gccagcgcca cccaaataaa ggcgaaagtc accatcttat
      541 cggcacccgg tttttcacca taaaacgtca cagccagcag gaacatcagc gtcgggccaa
      601 tgtactggaa aaagcctaac gttgagagac gcaagcgcgt ggcagcggcg gtaaaacaca
      661 acagcggtac ggtagtgaca ataccggcgg cgatcagcag taaattcagc gacatcgggt
      721 tttgccccat atggctggtt gagctgtcgg caatagcaaa caggtaaatt gccgccacgg
      781 gcagcagcca catggtttcg attaacatgc cggtttgcgc ttcaacggca atcttcttgc
      841 gtaccagacc gtagaaggca aaactaaatg ccagtcccag cgcgataata ggtagcgaac
      901 caaaagtcca cagctggact aacacgccac atatcgccag aatcaccgcc agccattgca
      961 tccggcggaa tcgctcgccg aggaaaatca tccccagcac aatgttcacc agcgggttaa
     1021 taaagtaacc aaggctcgct tccagcatat ggtgattgtt caccgcccag ataaacagta
     1081 gccagttgcc accaatcagc acggcagaga ctgccagcat aaaaattttc tgtggcgtct
     1141 gaatcagcgt ttttaaatag gaccactggc ggcaaatgct catcagcacc accataaaga
     1201 aaaacgacca gatcacgcga tgcgtcagga tttcatcggc gggcacgtag taaatcaact
     1261 tgaagtacgc tggcgctata ccccaaataa aataagcggc aagagcgagt aatacgccct
     1321 gccgcgtttg ttttgcatcc atcgggaata ctcattttta attggtaaca gcagtttacc
     1381 tgcttttatg tcttcaacct accatatagg tggcggtggc actggcaata taaagctgtt
     1441 cttcattgtg taattcaacg cgggcgacgg cgactttatt gcctgcacgc aacaggctac
     1501 tggtagcagt aaaacgctcg cccctgcctg ggcgcagata atcaacgcga agatcaatgg
     1561 tccccatccg cgatagccgc tggcgtagtt catcttcact gatggtttcg tggcgggtta
     1621 aggtacttcc cacgcacacc agaccggcgg cgacatccag cgccgacgca atgaccccgc
     1681 cgtgcaaaat gctttgcgcc cagttgccca ccatcattgg ctgattttta aaggccagct
     1741 gtgcgaactc tttttcgtaa cgctccagtt ccatccccaa tgcgcggtta aatggcatgt
     1801 gataaacaaa catctcaccc actaatttca gggcttgttc agcggtcagt acggcagaca
     1861 tatcatcctt acacttcatt ggttaatgaa atgttgattt tatgcttctt tgttgttggt
     1921 ttctacttta ggaagggata actaacggct atggagttaa gtatgtaaaa tagcccgcag
     1981 aaaaatattc accttatcaa taattcgtta cggagaacac gaccgatgcg gactctgcag
     2041 ggctggttgt tgccggtgtt tatgttgcct atggcagtat atgcacaaga ggcaacggtg
     2101 aaagaggtgc atgacgcgcc agcggtgcgt ggcagtatta tcgccaatat gctgcaggag
     2161 catgacaatc cgttcacgct ctatccttat gacaccaact acctcattta cacccaaacc
     2221 agcgatctga ataaagaagc gattgccagt tacgactggg cggaaaatgc gcgtaaggat
     2281 gaagtaaagt ttcagttgag cctggcattt ccgctgtggc gtgggatttt aggcccgaac
     2341 tcggtgttgg gtgcgtctta tacgcaaaaa tcctggtggc aactgtccaa tagcgaagag
     2401 tcttcaccgt ttcgtgaaac caactacgaa ccgcaattgt tcctcggttt tgccaccgat
     2461 tactgttttg caggttggac gctgcgcgat gtggagatgg ggtataacca cgactctaac
     2521 gggcgttccg acccgacctc ccgcagctgg aaccgccttt atactcgcct gatggcagaa
     2581 aacggtaact ggctggtaga agtgaagccg tggtatgtgg tgggtaatac tgacgataac
     2641 ccggatatca ccaaatatat gggttactac cagcttaaaa tcggctatca cctcggtgat
     2701 gcggtgttga gtgcgaaagg acagtacaac tggaataccg gttacggcgg cgcggagtta
     2761 ggcttaagtt acccgatcac caaacatgtg cgcctttata ctcaggttta cagcggctat
     2821 ggcgaatcgc tcatcgacta taacttcaac cagacccgtg tcggtgtagg agttatgcta
     2881 aacgacttgt tttgatgaac ggttgagtgg ttggcaaatc tggaacccag catccaggat
     2941 taccctctca gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg
     3001 tttttatttc aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa tctggagtcc
     3061 ggagctaaac aggttttaca agaaaccttt ggctaccaac agtttcgccc cggccaggaa
     3121 gaaattatcg acactgtgct ttccggtcgc gattgcctgg tcgtcatgcc caccggtggc
     3181 ggaaaatccc tttgctatca aattcctgcc ttattgctaa acggccttac cgtggttgtt
     3241 tcaccgctga tttcgttgat gaaagatcag gtggatcaac tgcaagccaa cggcgtggcg
     3301 gcggcgtgcc ttaactcgac gcaaacccgc gagcagcaac ttgaagtgat gacaggctgc
     3361 cgcaccgggc aaattcgtct gctttatatc gccccggaac gcctgatgct ggataacttt
     3421 cttgagcatc tggcgcactg gaatccggtg ttattagccg ttgatgaagc gcactgtatc
     3481 tcccaatggg gccacgattt ccgcccggaa tatgccgcgc ttggtcagtt acgccagcgt
     3541 ttcccgaccc tgccgtttat ggcgctgact gccacagccg acgacaccac gcgccaggat
     3601 atcgtgcgct tgctggggct gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg
     3661 aatattcgct acatgctgat ggagaaattc aaaccgctcg atcagttgat gcgctacgtg
     3721 caggaacagc gcggtaagtc cggcattatc tactgcaaca gccgggcgaa agtagaagac
     3781 accgctgcgc gcctgcaaag caagggtatt agcgcggcgg cctatcatgc cgggctggaa
     3841 aataatgtcc gcgccgacgt gcaggagaaa ttccagcgcg atgacctgca aattgtggtg
     3901 gcgacggtgg cgttcggcat gggcatcaat aaaccaaacg ttcgcttcgt ggtccacttt
     3961 gatattccgc gcaatatcga atcctattat caggaaaccg gtcgcgccgg gcgtgatggc
     4021 ctgcctgcgg aagcgatgct gttttacgat ccggctgata tggcgtggct gcgccgttgt
     4081 ctggaagaga agccgcaggg gcagttgcag gatattgagc gccacaaact caatgcgatg
     4141 ggcgcgtttg ccgaagcgca aacttgccgt cgtctggtat tgctgaacta ttttggcgaa
     4201 gggcgtcagg agccgtgcgg gaactgcgat atctgcctcg atccgccgaa acagtacgac
     4261 ggttcaaccg atgctcagat tgccctttcc accattggtc gtgtgaatca gcggtttggg
     4321 atgggttatg tggtggaagt gattcgtggt gctaataacc agcgtatccg cgactatggt
     4381 catgacaaac tgaaagtcta tggcatgggc cgtgataaaa gccatgaaca ttgggtgagc
     4441 gtgatccgcc agctgattca cctcggcctg gtgacgcaaa atattgccca gcattctgcc
     4501 ctacaactga cagaggccgc gcgcccggtg ctgcgcggcg aatcctcttt gcaacttgcc
     4561 gtgccgcgta tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat
     4621 gatcgcaaac tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga tgaaagtaat
     4681 gtcccgccgt acgtggtgtt taacgacgca accttgattg agatggctga acagatgccg
     4741 atcaccgcca gcgaaatgct cagcgttaac ggcgttggga tgcgcaagct ggaacgcttt
     4801 ggtaaaccgt ttatggcgct gattcgtgcg catgtcgatg gcgacgacga agagtagtca
     4861 gcagcataaa aaagtgccag gatgaagact ccgtaaacgt ttcccccgcg agtcaaatgt
     4921 atgttgatgt tatttctcac cgtcgccatg gtgcacattg tggcgcttat gagccccggt
     4981 cccgatttct tttttgtctc tcagaccgct gtcagtcgta cccgtaaaga agcgatgatg
     5041 ggcgtgctgg gcattacctg cggtgtaatg gtttgggctg ggattgcgtt gcttggcctg
     5101 catttgatta tcgaaaaaat ggcctggctg catacgctta tggttggcgg tggcctgtat
     5161 ctctgctgga tgggttacca gatgctgcgc ggcgcgctga aaaaagaggc ggtttctgca
     5221 cctgcgccac aggtcgagct ggcgaaaagt gggcgcagtt tcctgaaagg tttactgacc
     5281 aatctcgcta atccgaaagc gattatctac tttggctcgg tgttctcatt gtttgtcggt
     5341 gataacgttg gcactaccgc gcgctggggc atttttgcgc tgatcattgt cgaaacgctg
     5401 gcgtggttta tcgtcgttgc cagcctgttt gccctgccgc aaatgcgccg tggttatcaa
     5461 cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt tggcattcat
     5521 ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa ggcgtttacg
     5581 ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct cccaccagca
     5641 taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct ttaatccata
     5701 gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac aataatatcg accacaatag
     5761 tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg atgaattgcg
     5821 gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca aaaactgcgc
     5881 gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaaggtca attgcaccag
     5941 cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc cacttcaaca
     6001 cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca accagcacaa
     6061 tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg cgataaccgt
     6121 ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg cttaaaatga
     6181 tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc cggtgtgtct
     6241 atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgcta aaaaaaacga
     6301 ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga ctgggaaaca
     6361 agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg gcgtcagcgt
     6421 gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg ttttcgcgca
     6481 cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta cgtaaaatat
     6541 gcggaactgg cctatgacct gttccatttg gggtttgatg tcttaatcat cgaccatcgc
     6601 gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt taatcgcttt
     6661 aatgattatg ttgatgatct ggcggcattc tggcagcagg aggttcagcc cggtccgtgg
     6721 cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt atttctgcaa
     6781 cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg gatcgtgatt
     6841 cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca tccacgtttc
     6901 cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc tatcaacgta
     6961 ctgacccaca gcagacagcg atatcgacgt aacttacgct tctatgctga tgacccaacg
     7021 attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc tggcgaacag
     7081 gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttgcaggc tgaagaggaa
     7141 cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac tccgcaccgc cgcgggccat
     7201 cctgtcgaag gaggacgacc gttggtaatt aaaggtgctt accatgagat cctttttgaa
     7261 aaggacgcaa tgcgctcagt cgcgctccac gccattgttg attttttcaa caggcataac
     7321 tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg ttgttgcgtc
     7381 tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg ccaaagaaac
     7441 cctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc gtcaccacgt
     7501 tgatgtgggg caaattcgcg ataatctgga gatcaagtcg tacatgatca cctccaacgg
     7561 cgcgcgcgtt cacgatctgg atggcaatct gatttttgct cataacctgg atcgcgacat
     7621 tgccagcgat ctgtttggcg tagtcaacga caatctggac atcattacta acgtttatcg
     7681 cgacgacgaa tggtttatga atcgccatcg cccggaagag atgcgctttt ttaaagaagc
     7741 ggtgttcaaa tatgcgctgt atgagcctgg attactggag ccggaaggcg tcagcaaagt
     7801 gttcttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga ttaacgctcg
     7861 ttggggcgat cgcgtcaacg ttagtttctc taccttaacc tgtctggaag tgatggcggg
     7921 cggcgtttca aaaggccatg cgctggaagc ggtggcgaag aaactgggct acagcctgaa
     7981 ggattgtatt gcgtttggtg acgggatgaa cgatgccgaa atgctgtcga tggcggggaa
     8041 aggctgcatt atgggcagtg cgcaccagcg tctgaaagac cttcatcccg agctggaagt
     8101 gattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct atttatcgta
     8161 atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat actcgttaat
     8221 caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg ccttctcctt
     8281 tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg tgctggtgcg
     8341 cgttggcctg gcggcactcg tttttctgcc gtttctgcgt acccgtggca atagcctgaa
     8401 aacggtcggc ctgtatatgc tggtgggcgc gatgcagctc ggcgtgatgt atatgctgag
     8461 tttccgcgct tatctctacc tgacggtctc cgaattgttg ctgttcaccg tactgacgcc
     8521 gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct ggggctatgc
     8581 ttttagcgcc ttgctggcgg tgattggtgc cgggattatt cgctatgatc aggttaccga
     8641 ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg ccatcggcat
     8701 ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg cctttgcgtg
     8761 gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg gaaatgcgca
     8821 gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg tggtggcttc
     8881 cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg gaacgctggg
     8941 cattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta tctggcacca
     9001 acagccgcac tggccaacgt tcattacagg cgcgctggtg atcctggcct cactgtgggt
     9061 gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca ggcgtgattg
     9121 cgcgctgagc gaataaatgc ttctgtaatc ggctgacgct gctcgccatc gcgcacggcg
     9181 gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag accctggcgc
     9241 tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc ggcaaccatc
     9301 tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc tgccggctga
     9361 agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaatcaa cagcgtctcg
     9421 ctggcgagat cttccggtgt aattcgcgtt ttcgccgcca gtggatggtc aggtgctaac
     9481 accagacgca cttcatagtc gaacatcggc gaataatgca ggccactgcg cggcaggata
     9541 tcggaggtca tcaccagatc cagctcgccc tgttgcaacg caggctgcgg gtcaaacgtc
     9601 acgcccgatt taaaatccat ctctacctgc ggccagttct tatggaaatt ttctaacgcg
     9661 ggcgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg cgtctgctgc
     9721 ggttcattgc aggcttgcag tgcctggctg atttgcggca gcacctggtt tgccagttgc
     9781 aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac aaatagccgg
     9841 aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg cgtctgatgc
     9901 aacgtcgccg cagcggctgc gagagagccg cagttccgca acgcttgtag cgttttcagg
     9961 tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg cttgaggaat
    10021 atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaaatc cttcgtcttt
    10081 taaatttatg gtgcgttggc tgcgtttctc caccccggtc acttacttca gtaagctccc
    10141 ggggatgaat aaacttgccg ccttccctaa attcaaaatc cataggattt acatataatt
    10201 agaggaagaa aaatgacaat attgaatcac accctcggtt tccctcgcgt tggcctgcgt
    10261 cgcgagctga aaaaagcgca agaaagttat tgggcgggga actccacgcg tgaagaactg
    10321 ctgacagtag ggcgtgaact gcgtgctcgt cactgggatc aacaaaagca agcgggtatc
    10381 gacctgctgc cggtgggcga ttttgcctgg tacgatcatg tactgaccac cagtttgctg
    10441 ctgggtaatg ttccgccacg tcatcagaac aaagacggtt cggtagatat cgacaccctg
    10501 ttccgtattg gtcgtggacg tgcgccgact ggcgaacctg cggcggcagc ggaaatgacc
    10561 aaatggttta acaccaacta tcactacatg gtgccggagt tcgttaaagg ccaacagttc
    10621 aaactgacct ggacgcagct gctggaggaa gtggacgagg cgctggcgct gggccacaac
    10681 gttaagcctg tgctgctggg gccggttact tacctgtggc tggggaaagt gaaaggtgaa
    10741 cagtttgacc gccttagcct gctgaacgac attctgccgg tttatcagca agtgctggca
    10801 gaactggcga aacgcggcat cgagtgggta cagattgatg aacccgcgct ggtactggaa
    10861 ctaccacagg cgtggctgga cgcatacaaa cccgcttacg acgcgctcca gggacaggtg
    10921 aaactgctgc tgaccaccta ttttgaaggc gtaacgccaa atctcgacac gattactgcg
    10981 ctgcctgttc agggtctgca tgttgatctc gtacatggta aagatgacgt tgctgaactg
    11041 cacaagcgcc tgccttctga ctggttgctg tctgcgggtc tgatcaatgg tcgtaacgtc
    11101 tggcgcgccg atcttaccga gaaatatgcg caaattaagg acattgtcgg caaacgtgat
    11161 ttgtgggtgg catcttcctg ctcgttgctg cacagcccca tcgacctgag cgtggaaacg
    11221 cgtcttgatg cagaagtgaa aagctggttt gccttcgccc tacaaaaatg ccatgaacta
    11281 gcactgctgc gcgatgcgct gaacagtggt gacacggcag ctctggcaga gtggagcgcc
    11341 ccaattcagg cgcgtcgtca ctctactcgt gtacataatc cggcggtaga aaagcgtctg
    11401 gcggcgatca ccgcccagga cagccagcgt gcgaatgtct atgaagtgcg tgctgaagcc
    11461 cagcgtgcac gttttaaact gccagcgtgg ccgaccacca cgattggttc cttcccgcaa
    11521 accacggaaa ttcgtaccct gcgtctggat ttcaaaaagg gcaatctcga cgccaataac
    11581 taccgcacgg gcattgcgga acatatcagg caggccattg ttgaacagga acgtttggga
    11641 ctggatgtgc tggtacatgg cgaggccgag cgtaatgaca tggtggaata ctttggcgag
    11701 cacctcgacg ggtttgtctt tacgcaaaac ggttgggtac agagctacgg ttcccgctgc
    11761 gtgaagccac cgattgttat tggtgacgtt agtcgcccgg caccgattac cgtggagtgg
    11821 gcgaagtatg cgcaatctct gactgataaa ccggtgaaag ggatgctgac tggcccggtg
    11881 actattctct gctggtcgtt cccgcgtgaa gatgtcagcc gtgaaaccat cgccaaacag
    11941 attgcgctgg cgctgcgtga tgaagtggcc gatctggaag ccgctggaat tggcatcatc
    12001 cagattgacg aaccggctct gcgcgaaggt ttaccgctgc gtcgtagcga ctgggatgcg
    12061 tatctccagt ggggcgtaga ggccttccgt atcaacgccg ccgtggcgaa agatgacaca
    12121 caaatccaca ctcacatgtg ttattgcgag ttcaacgaca tcatggattc gattgcggca
    12181 ctggacgcag acgtcatcac catcgaaacc tcgcgttctg acatggagtt gctggagtcg
    12241 ttcgaagagt ttgattatcc gaatgaaatc ggtccaggcg tctatgacat tcactcgcca
    12301 aacgtaccga gcgtggaatg gattgaagcc ttgctgaaga aagcggcaaa acgcattccg
    12361 gcagagcgcc tgtgggttaa cccggactgt ggcctgaaaa cgcgcggctg gccagaaacc
    12421 cgcgcggcac tggcgaacat ggtgcaggcg gcgcagaatt tgcgtcgggg atgatgaata
    12481 ttaacgagtc actgtaaatt gcagtacgat gaaagaggca aaactgcctg atactaggcc
    12541 tacataattt atgcaattta ttgaattttt ggattttgtt ggccggacaa ggcgttcacg
    12601 ccgcatccgg catgaacaac gcgcactttg ccaataatct gatggcttcc atcaccggaa
    12661 gccattttgc tatctgcgcc cgttcagata cagaaataac cgaatcattt ttacgcaaaa
    12721 tataatcctg agcgatttgt tcagtaatca acgctacgtt atttattctc acttttcacg
    12781 aaattgtatc ccttttcagt aacgtggtag tgaggcatag acttatgtcc gctatcctca
    12841 ataataacca aaccctcgtt agctaatttg ttaacaatgt taacaacatt accgaatcct
    12901 ggaatttgcc ttatggatag tgtgcgatcg agattcatcc agttccagtt tatatctttc
    12961 tcagccataa ttttcagtac gttaatttca gtttcagtta tcatagtcaa gtgcatcctg
    13021 gtggtataag tcattaagtt ctcccttaag tttataatct tctagagtag cagtatgcgt
    13081 a
//
LOCUS       DAZPEP010000115        12998 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZPEP010000115 DAZPEP010000000
VERSION     DAZPEP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12998
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.115"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..191
                     /locus_tag="ACHUT9_003112"
     CDS             <1..191
                     /locus_tag="ACHUT9_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349525.1"
                     /translation="FHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQ
                     AGCPTVYPLALASTSVKN"
     gene            191..1393
                     /locus_tag="ACHUT9_003113"
     CDS             191..1393
                     /locus_tag="ACHUT9_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFR1349526.1"
                     /translation="MNLSANAQATLLLTSDFSRAAASEYKPLSNSEWGKFALWLKHQR
                     ISPAELLVPQPQEKLTGWSDPRISQERILGLLARGHSLVLAVDKWQRAGLWILTRGDA
                     DYPVRLKNRLRTDAPPVLFGCGNKALLQAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQ
                     GICVISGGARGIDECAMASALEAGGTAVGVLADSLLKTSTLVKWREGLIAGNLVLISP
                     FYPEVRFTVGNAMARNKYIYCLAESAMVVRAGMTGGTITGAMEALKHQWLPVQVKPNQ
                     DMQSANSRLVENGASWSAEQAENVTIRLPDVPGLMYDRALRNAQPELFSLHEDDANYA
                     VMPAYTPVDFYQLFVAELAILAKESISIERLASCTGLTIEQISVWLNRAEEEGRVIRL
                     GEGHYQFR"
     gene            complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUT9_003114"
     CDS             complement(1439..2032)
                     /gene="yhgN"
                     /locus_tag="ACHUT9_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFR1349527.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2224..3327
                     /gene="asd"
                     /locus_tag="ACHUT9_003115"
     CDS             2224..3327
                     /gene="asd"
                     /locus_tag="ACHUT9_003115"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFR1349528.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUT9_003116"
     CDS             3600..5786
                     /gene="glgB"
                     /locus_tag="ACHUT9_003116"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFR1349529.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUT9_003117"
     CDS             5783..7756
                     /gene="glgX"
                     /locus_tag="ACHUT9_003117"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFR1349530.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUT9_003118"
     CDS             7774..9069
                     /gene="glgC"
                     /locus_tag="ACHUT9_003118"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFR1349531.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUT9_003119"
     CDS             9069..10502
                     /gene="glgA"
                     /locus_tag="ACHUT9_003119"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFR1349532.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUT9_003120"
     CDS             10521..12968
                     /gene="glgP"
                     /locus_tag="ACHUT9_003120"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFR1349533.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3039 a         3226 c         3576 g         3157 t
ORIGIN      
        1 gtttccacca gtgtcaaaat ctcgacgggg cgtttgttat tacccctcct ttgatgcctg
       61 gtccggcgtt gctggttgac gatatcgtgg attctgcatg gacgctgaca gttctgacag
      121 cactgttacg ccaggcaggt tgcccgacgg tttatcctct tgcccttgct tctacctcgg
      181 taaaaaattg atgaatcttt cagccaatgc acaagcaact ctcctgctaa ccagcgattt
      241 ttctcgcgcg gcggcgagtg agtataaacc tcttagtaat agtgaatggg ggaagtttgc
      301 attatggctg aagcaccaac gtatcagtcc cgccgagctt ctggtgccgc aaccgcaaga
      361 gaaacttaca ggctggagcg atccgcgtat ttctcaggag cgtattcttg gcttgctggc
      421 gcgtggtcat agtctggtgt tggcggtaga taagtggcaa cgcgccggtt tatggatctt
      481 aacccgcgga gatgctgatt atcccgttcg cttgaaaaac cgattgcgaa cggatgcacc
      541 tcccgtttta tttggctgcg ggaataaagc attactgcaa gcggaaggta tggcgattgt
      601 tggctcgcga gatgctccga ctgacgattt gcgctatacc caacaactgg ccgcgaaact
      661 ggcccaacag gggatttgcg ttatctctgg tggtgcgcga ggtattgatg aatgtgcaat
      721 ggcgtcggca ctggaggccg ggggaactgc cgttggcgta ttagctgata gcttgttaaa
      781 aacgagtacg ttagtgaaat ggcgtgaagg gcttatagca ggcaacctgg tgttgatttc
      841 gccgttttac ccagaggtac gtttcaccgt cggcaatgcg atggcgcgaa ataaatatat
      901 ttattgcctt gctgaaagcg caatggttgt acgtgcggga atgaccggtg gaacgataac
      961 cggggcgatg gaggcattaa aacatcagtg gctgcctgtg caggttaaac caaatcagga
     1021 tatgcaatca gccaattcac gattagtaga aaatggggcg tcatggagtg ctgaacaggc
     1081 tgagaatgtg acgatcagac tgccagacgt tcctgggctg atgtatgaca gagcactccg
     1141 taacgctcaa ccagaactgt tttcgctgca tgaagatgac gcaaattacg cagtaatgcc
     1201 cgcgtatacg cctgtcgatt tttatcaact ctttgtggcg gaactggcga tccttgcaaa
     1261 ggaatcgata agtattgaaa ggctggcgtc ttgtactggt ttaaccatcg aacaaattag
     1321 tgtgtggctg aaccgcgcag aagaagaggg aagggttatc cgattgggcg aaggtcatta
     1381 tcagttcagg taataaaaca ggtgccgcgt ctggttgtgt gcagcacctg tttgaacctt
     1441 accccttcat ccacattcga atgccgtcga ggaacatctg ggttgccatc atcaccagaa
     1501 tcaatcccat caggcgttca agtgcgttca cccctttctc gcccagcaga cgtaaaaata
     1561 gcgaagactg tagcaggatg acaaaggtgc cgccccaggc cagcagcaga gcaatcacca
     1621 gatgtcccat ctgattcggg tactgatgag acaacaacat cagcgtggcg agaatagtcg
     1681 gcccggcgac taacggaatt gccaacggca cgataaatgg ctcttcacct gccggaagcc
     1741 cgctgctatt tcctgaagcg ctggggaaaa tcatcttaat ggcgattaga aacagaatga
     1801 tgccgccaga aatggagacg gtttctgctc gcaggctgag aaatgccaga attttctcac
     1861 ccgcaaacag gaacaccagc attaccagga gagcaataag caactctcgc accatgattg
     1921 cccgccgtct tttcggttca gtatgtttca gtacggacat gaaaataggc aggtttccga
     1981 gcggatccat aatcaggatc aataaaactg ctgcagaaat gatttcattc ataactcaaa
     2041 ttccctgata attgccgcgg actttctgcg tgctaacaaa gcaggataag tcgcattact
     2101 gatggcttcg ctatcattga ttaatttcac ttgcgacttt ggctgctttt tgtatggtga
     2161 aagatgtgcc aagaggagac cggcacattt atacagcaca catctttgca ggaaaaaacg
     2221 cttatgaaaa atgttggttt tatcggctgg cgcggtatgg tcggctccgt tctcatgcaa
     2281 cgcatggttg aagagcgcga cttcgacgcc attcgccctg tcttcttttc tacttctcag
     2341 cttggccagg ctgcgccgtc ttttggcgga accactggca cacttcagga tgcctttgat
     2401 ctggaggcgc taaaggccct cgatatcatt gtgacctgtc agggcggcga ttataccaac
     2461 gaaatctatc caaagcttcg tgaaagcgga tggcaaggtt actggattga cgcagcatcg
     2521 tctctgcgca tgaaagatga cgccatcatc attcttgacc ccgtcaatca ggacgtcatt
     2581 accgacggat taaataatgg catcaggact tttgttggcg gtaactgtac cgtaagcctg
     2641 atgttgatgt cgttgggtgg tttattcgcc aatgatcttg ttgattgggt gtccgttgca
     2701 acctaccagg ccgcttccgg cggtggtgcg cgacatatgc gtgagttatt aacccagatg
     2761 ggccatctgt atggccatgt ggcagatgaa ctcgcgaccc cgtcctctgc tattctcgat
     2821 atcgaacgca aagtcacaac cttaacccgt agcggtgagc tgccggtgga taactttggc
     2881 gtgccgctgg cgggtagcct gattccgtgg atcgacaaac agctcgataa cggtcagagc
     2941 cgcgaagagt ggaaagggca ggcggaaacc aacaagatcc tcaacacatc ttccgtaatt
     3001 ccggtagatg gtttatgtgt gcgtgtcggg gcattgcgct gccacagcca ggcattcact
     3061 attaaattga aaaaagatgt gtctattccg accgtcgaag aactgctggc tgcgcacaat
     3121 ccgtgggcga aagtcgttcc gaacgatcgg gaaatcacta tgcgtgagct aaccccagct
     3181 gccgttaccg gcacgctgac cacgccggta ggccgcctgc gtaagctgaa tatgggacca
     3241 gagttcctgt cagcctttac cgtgggcgac cagctgctgt ggggggccgc ggagccgctg
     3301 cgtcggatgc ttcgtcaact ggcgtaatct ttattcatta aatctggggc gcgatgccgc
     3361 ccctgttagt gcgtaataca ggagtaagcg cagatgtttc atgatttacc gggagttaaa
     3421 tagagcattg gctattcttt aagggtggct gaatacatga gtattcacag ccttacctga
     3481 agtgaggacg acgcagagag gatgcacaga gtgctgcgcc gttcaggtca aaaaaatgtc
     3541 acaaccagaa gtcaaaaatc caattggatg gggtgacaca ataaaacagg aagacaagca
     3601 tgtccgatcg tatcgataga gacgtgatta acgcgctaat tgcaggccat tttgcggatc
     3661 ctttttccgt actgggaatg cataaaacca ccgcgggact ggaagtccgt gcccttttac
     3721 ccgacgctac cgatgtgtgg gtgattgaac cgaaaaccgg gcgcaaactc gcaaaactgg
     3781 agtgtctcga ctcacgggga ttctttagcg gcgtcattcc gcgacgtaag aattttttcc
     3841 gctatcagtt ggctgttgtc tggcatggtc agcaaaacct gattgatgat ccttaccgtt
     3901 ttggtccgct aatccaggaa atggatgcct ggctattatc tgaaggtact cacctgcgcc
     3961 cgtatgaaac cttaggcgcg catgcagata ctatggatgg cgtcacaggt acgcgttttt
     4021 ctgtctgggc tccaaacgcc cgtcgggtct cggtggttgg gcaattcaac tactgggacg
     4081 gtcgccgtca cccgatgcgc ctgcgtaaag agagcggcat ttgggaactg tttatccctg
     4141 gggcgcataa cggtcagctc tataaatacg agatgattga tgccaatggc aacttgcgtc
     4201 tgaagtccga cccttatgcc ttcgaagcgc aaatgcgccc ggaaaccgcg tctcttattt
     4261 gcgggctgcc ggaaaaggtt gtacagactg aagagcgcaa aaaagcgaat cagtttgatg
     4321 cgccaatctc tatttatgaa gttcacctgg gctcctggcg tcgccacacc gataacaatt
     4381 tctggttgag ctaccgcgag ctggccgatc aactggtgcc ttatgctaaa tggatgggct
     4441 ttacccacct cgaactactg cccattaacg agcatccctt cgatggcagt tggggttatc
     4501 agccaaccgg cctgtatgca ccaacccgcc gttttggtac tcgcgacgac ttccgttatt
     4561 tcattgatgc cgcacacgca gcgggtctga acgtgattct cgactgggtg ccaggccact
     4621 tcccgaccga tgactttgcg cttgccgaat ttgatggcac gaacttgtat gaacacagcg
     4681 atccgcgcga aggctatcat caggactgga atacgctgat ctacaactat ggtcgccgtg
     4741 aagtcagtaa cttcctcgtc ggtaacgcgc tttactggat tgaacgtttt ggtattgatg
     4801 cgctgcgcgt cgatgcggtg gcgtcaatga tttatcgcga ctacagccgt aaagaggggg
     4861 agtggatccc gaacgaattt ggcgggcgcg agaatcttga agcgattgaa ttcttgcgta
     4921 ataccaaccg tattcttggt gagcaggttt ccggtgcggt gacaatggct gaggagtcta
     4981 ccgatttccc tggcgtttct cgtccgcagg atatgggcgg tctgggcttc tggtacaagt
     5041 ggaacctcgg ctggatgcat gacaccctgg actacatgaa gctcgacccg gtttatcgtc
     5101 agtatcatca cgataaactc accttcggga ttctctacaa ctacactgaa aacttcgtcc
     5161 tgccgttgtc gcatgatgaa gtggtccacg gtaaaaaatc gattctcgac cgcatgccgg
     5221 gcgacgcatg gcagaaattc gctaacctgc gcgcctacta tggctggatg tgggcattcc
     5281 cgggcaagaa actgctgttc atggggaacg aatttgccca aggccgtgag tggaaccatg
     5341 acgccagcct cgactggcat ctgttggaag gcggcgataa ctggcaccac ggtgtccagc
     5401 gtctggtgcg cgatctgaac ctcacctacc gccaccataa agcaatgcat gaactggatt
     5461 ttgacccgta cggctttgaa tggctggtgg tggatgacaa agaacgctcg gtgctgatct
     5521 ttgtgcgtcg cgataaagag ggtaacgaaa ttatcgttgc cagtaacttt acgccggtgc
     5581 cgcgtcatga ttatcgcttc ggcattaatc agccaggcaa atggcgtgaa atcctcaata
     5641 ccgattccat gcactatcac ggcagtaatg caggcaatgg cggcacggta cacagcgatg
     5701 agattgccag ccacggtcgt cagcattcac taagcctgac actaccaccg ctggccacta
     5761 tctggctggt tcgggaggca gaatgacacg actcgccatt ggcaaacccg ctcccctcgg
     5821 cgcgcattac gacggtcagg gcgtcaactt cacacttttc tccgctcatg ccgagcgggt
     5881 agagctgtgt gtctttgacg ccaatggcca ggaacatcgc tatgacttgc cagggcacag
     5941 tggcgacatt tggcacggtt atctgccgga tgcgcgcccg ggtttgcgtt atggttatcg
     6001 cgttcatggc ccctggcaac ccgccgaggg gcatcgcttt aacccggcga agttgttgat
     6061 tgatccttgc gcgcggcaaa ttgaagggga attcaaagat aacccgctgc tgcacgccgg
     6121 tcataatgaa cctgactatc gcgacaacgc cgccattgcg ccgaaatgcg tagtggtggt
     6181 tgatcactat gactgggaag atgatgcccc gccgcgcacg ccgtggggca gcaccatcat
     6241 ttatgaagcc catgttaaag gattaacgta cctgcacccg gagatcccgg tcgagatccg
     6301 tggcacttat aaagccctcg ggcatccggt gatgatcaac tatttgaaac agttgggcat
     6361 taccgcgctg gaactgctgc cggtggcgca gtttgccagt gaaccacgtc tgcaacgcat
     6421 ggggctaagt aactactggg gttacaaccc ggtggcgatg tttgcgctgc atccggcgta
     6481 tgcctgctcg ccagaaacgg cgctgcatga gtttcgcgat gcaatcaaag cactgcataa
     6541 agcgggtatc gaagtcattc ttgatatcgt gctcaaccat agtgcggaac tggacctcga
     6601 cggcccgtta ttctcgctgc gtgggatcga caaccgtagc tattattgga taagagaaga
     6661 cggcgattat cacaactgga ccggttgtgg caacacgctc aatttgagtc atccggcggt
     6721 ggtggattat gcctgcgcct gcctgcgtta ttgggtagaa acctgtcacg tcgatggttt
     6781 ccgctttgat ctggcggcag tcatgggccg tacgccagag ttccgtcagg atgcgccgtt
     6841 gtttaccgct atccagaact gcccggtgct ctcgcaggtg aagttaattg ctgaaccgtg
     6901 ggatatcgcc cttggtggtt atcaggtggg aaatttcccg ccgctgtttg ccgagtggaa
     6961 cgatcatttc cgcgatgctg cccgtcgttt ctggctacat tatgatttgc ctctgggggc
     7021 gtttgccggg cgttttgctg cctccagcga tgtttttaaa cgtaatggtc gtctgccgag
     7081 tgccgcgatt aatctcgtca ctgcgcatga cggttttacg cttcgcgact gcgtttgctt
     7141 caaccataaa cacaatgaag caaacggaga agaaaatcgc gacgggacca acaacaatta
     7201 cagtaacaat catggtaaag aagggttagg cggttctctt gacctggttg aacggcggcg
     7261 cgacagtatt cacgccctgt taacaacgtt gttgctctcc cagggtacgc cgatgttact
     7321 ggccggtgac gaacacggtc acagccagca tggcaataac aatgcctact gtcaggataa
     7381 ccaattaacc tggttggact ggtcgcaggc aagcagtggt ttaaccgcat ttaccgccgc
     7441 gttaatccat ctgcgcaagc gcattcccgc tttggtggag aatcgctggt gggaagaagg
     7501 cgacggcaat gtccgttggc taaatcgata tgctcaacct ttaagcacgg atgagtggca
     7561 aaacgggccg aaacagctgc aaattctgct ctcggatcgc tttttgatcg caattaacgc
     7621 cacgcttgag gtaacagaga ttgttttacc tgctggggag tggcacgcca ttcccccatt
     7681 cgctggagag gataacccag tgattacggc tgtctggcag ggacctgcac acggattgtg
     7741 tgtgttccag agatgataaa aaaggagtta gtcatggtta gtttagagaa gaacgatcac
     7801 ttaatgttgg cgcgccagct gccattgaaa tctgttgccc tgatactggc gggaggacgt
     7861 ggtacccgcc tgaaggattt aaccaataag cgagcaaaac cggccgtaca cttcggcggt
     7921 aagttccgca ttattgactt tgcgctgtct aactgcatca actctgggat ccgtcgtatg
     7981 ggcgtgatca cccagtacca gtcccacact ctggtgcagc acattcagcg cggctggtca
     8041 ttcttcaatg aagaaatgaa cgagtttgtc gatctgctgc cagcacagca gagaatgaaa
     8101 ggggaaaact ggtatcgcgg caccgcagat gcggtcaccc aaaacctcga cattatccgc
     8161 cgttataaag cggaatacgt ggtgatcctg gcgggcgacc atatctacaa gcaagactac
     8221 tcgcgtatgc ttatcgatca cgtcgaaaaa ggcgcacgtt gcaccgttgc ttgtatgcca
     8281 gtaccgattg aagaagcctc cgcatttggc gttatggcgg ttgatgagaa cgataaaatt
     8341 atcgaattcg ttgaaaaacc tgctaaccca ccgtcaatgc cgaacgatcc gagcaaatct
     8401 ctggcgagta tgggtatcta cgtctttgac gccgactatc tgtatgaact gctggaagaa
     8461 gacgatcgtg atgagaactc cagccacgac tttggcaaag atttgattcc caagatcacc
     8521 gaagccggtc tggcctatgc gcacccgttc ccgctctctt gcgtacaatc cgacccggat
     8581 gccgagccgt actggcgcga tgtgggtacg ctggaagctt actggaaagc gaacctcgat
     8641 ctggcctctg tggtgccgga actggatatg tacgatcgca attggccaat tcgcacctac
     8701 aatgaatcat taccgccagc gaaattcgtg caggatcgct ccggtagcca cgggatgacc
     8761 cttaactcac tggtatccgg cggctgtgtg atctccggtt cggtggtggt gcagtccgtt
     8821 ctgttctcgc gcgttcgcgt gaattcattc tgcaacattg attccgccgt attgttaccg
     8881 gaagtatggg taggtcgctc gtgccgtctg cgccgctgcg tcatcgatcg tgcttgtgtt
     8941 attccggaag gcatggtgat tggtgaaaac gcagaggaag atgcacgtcg tttctatcgt
     9001 tcagaagaag gcatcgtgct ggtaacgcgc gaaatgctac ggaagttagg gcataaacag
     9061 gagcgataat gcaggtttta catgtatgtt cagagatgtt cccgctgctt aaaaccggcg
     9121 gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc gttgacgctc
     9181 gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcg caggtagtat
     9241 cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac aacggggttg
     9301 gcatatacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg tatcacgata
     9361 ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gcttctgggg tgggttgggg
     9421 cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat gcgcacgact
     9481 ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtcctgcg aagtcggtgt
     9541 ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg aatgacatcc
     9601 aattgccatg gtcattcttt aatattcatg ggctggaatt caacggacaa atctctttcc
     9661 tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc tacgctcgcg
     9721 agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag cgtcatcgtg
     9781 aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt ccagagacgg
     9841 acttactgtt ggcctcgcgc tacacccgcg atacgttgga agataaagcg gaaaataagc
     9901 gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt tttgcagtgg
     9961 tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta ccgggtcttc
    10021 tggagcaggg cgggcagctg gcgctactcg gcgcgggcga tccggtgctg caggaaggtt
    10081 tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc tatcacgaag
    10141 cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc cgttttgaac
    10201 cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta gtgcggcgca
    10261 ccggtgggct tgctgatacg gtttctgact gttctcttga gaaccttgca gatggcgtcg
    10321 ccagtgggtt tgtctttgaa gataataatg cctggtcgct gttacgggct attcggcgtg
    10381 cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag gctatggcaa
    10441 tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat cgcttgaaat
    10501 agttttcagg aaacgcctat atgaatgctc cgtttacata ttcatcgccc acgcttagcg
    10561 tagaagctct taagcactct atcgcttaca agctgatgtt tacgattgga aaggacccgg
    10621 tcgtcgccaa taaacatgaa tggctgaacg caacgttatt tgctgtgcgc gatcgtctcg
    10681 tggagcgctg gttacgttca aaccgtgccc agttgtcgca agaaacacgt caggtttact
    10741 acctgtcgat ggagtttttg attggccgta cgctctccaa cgccatgttg tcgctaggaa
    10801 tttacgaaga tgtacagggc gcactggaag cgatggggtt aaatctcgaa gagctgattg
    10861 atgaagaaaa tgacccaggc ctcggtaacg gtggcctggg acgtctggcg gcttgcttcc
    10921 ttgattctct ggcgacgtta gggttgccgg ggcgcggtta cggcatccgc tatgactacg
    10981 gtatgttcaa gcagaacatc gttaacggta gccagaaaga gtcgccagac tactggctgg
    11041 aatacggtaa cccgtgggaa ttcaaacgcc acaacacgcg ctataaagtc cgttttggcg
    11101 gtcgcattca gcaggaaggt aaaaaaacgc gctggattga aaccgaagag attctgggag
    11161 tcgcttacga tcagataatc cctggttacg acaccgacgc gaccaacacg ctgcgtttgt
    11221 ggagtgcgca agccagtagc gaaattaacc tcggtaaatt caaccagggt gactacttcg
    11281 cggcagtgga agataaaaac cactccgaga acgtatctcg cgtactgtat ccggatgact
    11341 ccacctactc cgggcgtgag ctgcgcctgc gtcaggaata cttcctggtt tcctcgacca
    11401 ttcaggacat tttaagccgc cattatcagt tgcataaaac ctacgataac ctggcggata
    11461 aaatcgcgat tcatctcaat gatacccatc cggtactgtc gattcctgag atgatgcgtc
    11521 tgctgatcga tgagcaccaa tttagctggg acgacgcgtt tgaggtgtgt tgtcaggtct
    11581 tctcctacac taaccacacg ctgatgagcg aggcgctgga aacctggccg gttgatatgc
    11641 tgggtaaaat tctgccgcgt cacctgcaga tcatctttga aatcaacgac tatttcctga
    11701 aaaccttgca ggaacagtat ccgaacgata ccgatctgct gggacgggcg tcgatcattg
    11761 atgaatccaa cggtcgtcgt gtgcgtatgg cctggctggc ggttgttgtg agccacaaag
    11821 ttaacggtgt atcggaactg cactctaatc tgatggtgca atcgttgttt gccgactttg
    11881 cgaaaatctt cccgggtcgt ttcaccaacg tcaccaacgg tgtgacgccg cgtcgctggc
    11941 tggcggtagc gaacccatcg ctttcagccg tgctggacga acacctgggc cgtaactggc
    12001 gcaccgacct tagcctgctt aatgagctgc aacaacactg tgatttccca atggttaatc
    12061 acgctgtgca tcaggcgaag ctggagaaca aaaagcgtct ggcagagtat atcgcccagc
    12121 agctgaatgt ggtggtgaat ccaaaggcgt tgttcgatgt acaaatcaaa cgtattcacg
    12181 aatacaaacg tcaattgatg aatgtgttgc atgtgattac ccgctataac cgcatcaagg
    12241 ccgacccgga tgcgaagtgg gtaccgcgcg tgaatatttt tggcggtaag gcggcttcgg
    12301 cctattacat ggcgaagcac attattcatt tgatcaatga cgtagcgaaa gtgatcaaca
    12361 acgatccgca gattggcgat aagctgaaag tcgtgttcat cccgaactac agcgttagcc
    12421 tggcgcagtt gatcattccg gcggcagatc tgtctgaaca gatttcgctg gcagggacgg
    12481 aagcttccgg caccagtaac atgaagtttg cgcttaacgg tgcgctgact atcggtacgt
    12541 tggacggtgc gaatgtcgag atgctggatc atgtcggtgc tgacaatatc tttatttttg
    12601 gtaacacagc ggaagaagtg gaagaactgc gtcgtcaggg ctacaaaccg cgtgaatact
    12661 acgagaaaga tgaggagctg catcaggtgc tgacgcaaat cggcagcggt gtattcagtc
    12721 cggaagatcc gggtcgctat cgcgatctgg ttgattcgct gatcaacttc ggcgatcact
    12781 atcaggttct ggcggattat cgcagctatg tcgattgtca ggataaagtc gacgaactct
    12841 acgagcttca ggaagagtgg accgcaaaag cgatgctgaa cattgccaat atgggctact
    12901 tctcttctga ccgtactatc aaagagtacg ccgatcatat ctggcatatc gatccggtga
    12961 gattgtaagt tcaccaataa atagaacggg gccaaagg
//
LOCUS       DAZPEP010000116        12563 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZPEP010000116 DAZPEP010000000
VERSION     DAZPEP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.116"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHUT9_003121"
     CDS             complement(<1..833)
                     /gene="gap"
                     /locus_tag="ACHUT9_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFR1349534.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IV"
     gene            1022..1552
                     /gene="cybB"
                     /locus_tag="ACHUT9_003122"
     CDS             1022..1552
                     /gene="cybB"
                     /locus_tag="ACHUT9_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFR1349535.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1797..1970
                     /locus_tag="ACHUT9_003123"
     CDS             1797..1970
                     /locus_tag="ACHUT9_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349536.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            1996..2064
                     /gene="yncP"
                     /locus_tag="ACHUT9_003124"
     CDS             1996..2064
                     /gene="yncP"
                     /locus_tag="ACHUT9_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFR1349537.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHUT9_003125"
     CDS             complement(2042..2191)
                     /gene="hokB"
                     /locus_tag="ACHUT9_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFR1349538.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            2590..4230
                     /gene="trg"
                     /locus_tag="ACHUT9_003126"
     CDS             2590..4230
                     /gene="trg"
                     /locus_tag="ACHUT9_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFR1349539.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(4268..5191)
                     /locus_tag="ACHUT9_003127"
     CDS             complement(4268..5191)
                     /locus_tag="ACHUT9_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFR1349540.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            5408..6709
                     /locus_tag="ACHUT9_003128"
     CDS             5408..6709
                     /locus_tag="ACHUT9_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1349541.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLY"
     gene            6976..8631
                     /gene="opgD"
                     /locus_tag="ACHUT9_003129"
                     /gene_synonym="mdoD"
     CDS             6976..8631
                     /gene="opgD"
                     /locus_tag="ACHUT9_003129"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFR1349542.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            8771..8995
                     /locus_tag="ACHUT9_003130"
     CDS             8771..8995
                     /locus_tag="ACHUT9_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFR1349543.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            9058..9594
                     /gene="rimL"
                     /locus_tag="ACHUT9_003131"
     CDS             9058..9594
                     /gene="rimL"
                     /locus_tag="ACHUT9_003131"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFR1349544.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHUT9_003132"
     CDS             complement(9589..10569)
                     /gene="ydcK"
                     /locus_tag="ACHUT9_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFR1349545.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            10693..11685
                     /gene="tehA"
                     /locus_tag="ACHUT9_003133"
     CDS             10693..11685
                     /gene="tehA"
                     /locus_tag="ACHUT9_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFR1349546.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            11682..12275
                     /gene="tehB"
                     /locus_tag="ACHUT9_003134"
     CDS             11682..12275
                     /gene="tehB"
                     /locus_tag="ACHUT9_003134"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFR1349547.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3208 a         2926 c         3146 g         3283 t
ORIGIN      
        1 actatttctt catcggtata accaaatgac tcgttattgg tggttgcttg tttaagtgcg
       61 ttattcacct cttcggcagt cacttttttt ccgagaatcg ataccagttc agtgaccgaa
      121 cctgttttca ccggcacgcg ttgcgcatga cctttcagtt tgccgctcag ttccgggatc
      181 accagaccaa tggcttttgc cgcccccgta gtgtggggaa tgatattttc tgccgctgcg
      241 cgtgaagcac gtaaatcttt accacgtggg ccatccacca gtgactgggt gccagtatag
      301 gcatgaatgg tcgtcatcgt gccgacttct atcccgaaac tgtcatgcaa ggctttggcc
      361 atcggcgcaa gacagttagt ggtgcatgac gccacggaaa caatggtgtc gttgccatcc
      421 agagtgtcgt cattgacgtt ataaacgata gttttcattt caccggcagg ggcggaaatc
      481 aacaccttct tcgcaccagc atcaagatgt gcctgcgatt tctcggcgga ggtataaaag
      541 ccagtacatt cgacaatgat ttctgcacct ttcgctttcc acggaatatt tttagcctct
      601 ttttcggcgt aaaccgcgat acttttccca tcaacgataa gtgaatcttc cgtaaaatca
      661 acgctccagg ggaatggtcc gtagtttgaa tcatgtttca gcaggtaggc gagaattttt
      721 ggggaagtga gatcattaat agcgacaacg tctatgttgc ttttgacttc aagtaatcga
      781 cgcaacacca gtcgaccgat acgaccaaaa ccgttaatac caactttact catggttttc
      841 tcctgtcagg aacattcgga tgaaaattga tcctttccag gcttagacca ggattgtggg
      901 atgggcaatc tccattctta cagtgaaacg taacgtactg aaaacgggtg aacaatattt
      961 aatgaaattt tgagaaaagc ccgttatgtt aacggaaaat tatgttaaag caggaaatgt
     1021 tatggaaaat aaatactcaa ggttacaaat cagcattcac tggctggtct ttttgctggt
     1081 tatcgcagcg tattgcgcaa tggagtttcg tggtttcttc ccacgtagcg atcggccact
     1141 catcaacatg attcatgttt cctgcggcat ctcaattctc gtgctgatgg tcgttcgtct
     1201 tttgttaagg ctgaaatacc caaccccgcc gattatacct aagccaaaac cgatgatgac
     1261 gggactggcg catttgggac atttggtgat gtatctcctg tttattgcgc tgccagtgat
     1321 tggtttggtg atgatgtata accggggcaa cccgtggttt gcgtttggtt tgacgatgcc
     1381 ttacgcttca gaggccaatt tcgaacgggt tgatagctta aagtcgtggc atgagacgct
     1441 ggcgaatctg ggatattttg tcatcgggtt gcacgctgcg gctgcactgg cacaccacta
     1501 tttctggaag gacaacacac ttctacgcat gatgccgcgt aaacgttcct gaaggatatt
     1561 tcaagaaaat gcctgtacta aaaccgaccc gtggtacagg cgaagaatac gggtctacat
     1621 cggaagcgcc tattatattt atttgtatga caaataaaac cataatcctt gcccatacgt
     1681 ccatctggct tatttttaat caattcaccc gatctttgat ctcatcaacg gtatcaaaat
     1741 aaaccagcgt ataacgttca aatcgaacat aagattcgat aaaccatgga ggttatatga
     1801 aaaaactggc acttattttg tttatgggaa cgcttgtttc cttttatgcc gatgccgggc
     1861 gcaaaccctg ttctggttcg aaagggggga tctcacactg tacggcaggc ggcaaatttg
     1921 tctgtaatga tggttctatt agtgcatcga aaaaaacatg cactaactga agtgtaaaag
     1981 gggtgccatg agaaaatgaa tctgctagtc agatgcgcgg ggaaaatccc cgcgcttgcc
     2041 cttacctgga cgtgcaggcc atgagcgcag caacctcctt atcaccgtcc cggaaccgca
     2101 gttcgtagag cgtttgtcgg gtcaggagtg tgaatgtcag aatcgtaatg cagataatga
     2161 gcagacacac taccagaggg ttgtgcttca tagcctttct ccttgccgga tggcgggtaa
     2221 gaggctaaga tctgaattgc taggttcatt cgttggcctc ggttgataga aatatcggtc
     2281 ggggccttcg tctttctgat tcccggttag cctgaaaaca gaaagtctca ggcacccgca
     2341 ggcatcctat gaggtttcct tagggacgaa aataatcact tcacgaaatt gcgtgctgtt
     2401 ttccagaatt tttcgtcatt cgggttagcc agtttagcca ttcgttactc tcttcattcc
     2461 aatagcatta attttctctg caataattgt tgtaaaaatg tgacgcaaag aggtttttgg
     2521 tcataagtaa ttaccgtcaa gtgccgatga ctttctatca ggagtaaacc tggacgagag
     2581 acaacggtaa tgaatacaac tccctcacag cgattaggtt ttttgcatca catcaggctg
     2641 gttccgttat ttgcctgcat tcttggcggt atcttagttc tattcgcatt aagttctgct
     2701 ctggctggct atttcctctg gcaggccgat cgcgatcagc gtgatgttac tgcggagatt
     2761 gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg gatcaatatg
     2821 attcaggccg gggctgcgag ttgtattgcg gaaatggaag caatgaagcg aaatattgcg
     2881 caagccgaat cggagattaa acagtcgcag caagggtatc gtgcttatca gaatcggccg
     2941 gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt tcaggcttat
     3001 atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga agcgattatc
     3061 aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat tttgaacaaa
     3121 gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca tcagcgcacc
     3181 cgcttgggtg ggatgttcat gattggcgcg tttgtgcttg ccctggtcat gacgctgata
     3241 acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc acaacggatt
     3301 gaaaaaatcg ctagtggcga tctgacgatg aaggatgaac cggcgggtcg taatgaaatc
     3361 ggtcgcttaa atcgtcattt acagcaaatg cagcattcac tggggatgac agtagggact
     3421 gttcgacagg gtgcggaaga gatttatcgt ggcaccagcg aaatttcagc tggcaatgcg
     3481 gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc cgccagcatg
     3541 gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc cagcaaactg
     3601 gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggtttccgg tgtagtaaaa
     3661 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     3721 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     3781 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     3841 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     3901 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     3961 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     4021 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     4081 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     4141 gaggcggtgg atgtattccg tctgcacaaa cattctgtgt cggcagaacc tcgcggagcg
     4201 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     4261 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     4321 agaacgtcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     4381 caggcagggc caccagcgtg gcatgacgca ggtcgtcttt tacagcgcca gaagggacaa
     4441 accacacgta atcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     4501 tacaacccga aggaatttta cagccctggc tctgcactaa tgcatctgaa tgctggcgtg
     4561 gcgcagtgcc ttctggtgat acaacgaccg gccattccag cacccggctt agcgttacgt
     4621 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     4681 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     4741 tttccccggt ttttaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     4801 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     4861 cagtaggtag tgcaccaacc ctgacgacat cattattaag accttcttta cgatgaagcg
     4921 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     4981 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     5041 gctccagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     5101 cgcgccccaa agttccttgt tgtgcgacag ctacgaatgt atgaaggtgg cgcaaacgga
     5161 tgcgctgact aaacagacta tttttttcca taagcgatgt taaaaacgaa gcggtgtcgc
     5221 tgacaagtga agttgtttga ttatgataac ttgattgcaa aatattatta acaattaaag
     5281 caattatgtt acagcaaaat ggataatatt gatgttttcg cggcgagatc acagtttgta
     5341 aattcttccc gccagagtga atgcggttac ctacactcca gattactgac cactggaggc
     5401 agacactatg gcgaacagca tcacggcgga tgagattcgg gaacagtttt cgcaggcaat
     5461 gtcagccatg taccagcaag aagttccgca atatggcacg ctgctggaac tggtagctga
     5521 tgtgaatctg gctgtgctgg aaaacaatcc tcaactgcac gaaaaaatgg taaatgcaga
     5581 cgagctggcg cgactgaatg ttgaacgtca tggggcgatt cgcgttggga ctgcacaaga
     5641 gcttgctact cttcggcgga tgtttgccat tatggggatg tacccggtga gctattacga
     5701 tctctcgcag gcaggggtgc cggtacattc gacagcattt cggcccattg atgatgcttc
     5761 tctggcgcgt aatcccttcc gcgtttttac ctccttactc cgccttgagc ttatcgagaa
     5821 cgaaattttg cgccagaaag cggcggagat tctgcgtcag cgcgatatct tcaccccacg
     5881 ttgtcgacaa ctgttagagg aatatgagca gcagggcggt tttaacgaaa cacaggcaca
     5941 ggagtttgtg caggaagccc tggaaacgtt tcgctggcac cagtcagcaa cggtagatga
     6001 agaaacctat cgcgccttgc acaacgaaca tcggttgatt gctgatgtgg tctgttttcc
     6061 tggatgccat atcaaccacc tgacgccacg tacgctggat attgaccggg tgcagtcgat
     6121 gatgcctgaa tgcggaattg aacccaaaat tctcatcgag gggccgccgc gccgcgaggt
     6181 accgatttta ctacgccaga ccagctttaa agcactggaa gagacggtgt tgtttgcggg
     6241 gcagaaacag ggcacgcata ccgcgcgctt tggtgaaatt gagcagcgtg gcgtggcatt
     6301 aacgccgaaa gggcgacaac tgtatgatga tcttctgcgt aacgctggaa ccgggcagga
     6361 taatctcact caccaaatgc atttacagga aaccttccgc acttttcctg acagtgagtt
     6421 tttaatgcgt cagcaagggt tggcatggtt ccggtaccgt ctgacgcctt cgggtgaggc
     6481 gcatcgtcag gcgattcatc ctggagacga tccacagccc ttaattgaac gtggttgggt
     6541 agtggcgcaa cccatcacct atgaagattt cttgcccgtt agcgcggcgg ggatcttcca
     6601 gtcaaatctg ggtaatgaaa cgcagacacg cagtcacggt aatgccagtc gcgaagcatt
     6661 tgagcaggcg ttgggttgtc cggttttgga tgagttccag ctttactagg aagcggaaga
     6721 acgcagtaaa cgtcgctgtg gtttgcttta aattctgacc atccgccttt gcaaaaattt
     6781 gcctgatttt acaaacgaat caggctcatc ccatcgacat aaaaaaaatg ccgatttatg
     6841 tatattctct cagttcaaca attggattat taataaatat tgtctagagt gagcggtcat
     6901 aaataagcac tttcttgccg ctgaaaacga ccagcgcggg accattcaca acaccagaag
     6961 gactcacttt caggtatgga tcgtagacga tttattaaag gttcaatggc tatggccgcc
     7021 gtgtgcggta ccagcggcat tgcttctctt ttttctcagg cggcattcgc ggcagattct
     7081 gatattgccg acgggcaaac ccagcgtttt gacttctcca ttctacagtc aatggcgcac
     7141 gacttagcgc aaacagcgtg gcgtggtgcg cctcgtccgt tacctgacac gctggcgaca
     7201 atgacgccgc aggcttataa cagtattcaa tacgacgccg aaaaatcgct ctggcataac
     7261 gttgagaacc gtcaactgga cgctcagttc ttccatatgg gaatgggatt ccgtcgccgc
     7321 gttcgtatgt tttctgtaga tccagcaaca catctggcgc gtgaaattca ctttcgcccg
     7381 gagttgttca aatacaacga tgcaggtgtt gataccaaac aattagaagg gcaaagcgat
     7441 ctcggctttg ccggttttcg cgtgtttaaa gcccccgaac tggcgcgccg tgatgtagta
     7501 tcatttctcg gcgcgagtta tttccgcgcc gttgatgata catatcaata cggtttgtcg
     7561 gcccgcggcc tggcgatcga cacttacacc gacagtaaag aagagttccc cgactttacc
     7621 gccttctggt ttgatacggt aaaaccgggg gcaactacct ttaccgttta tgcgttgctc
     7681 gatagcgcca gtattactgg tgcctataag ttcactatcc attgtgagaa aagtcaggtg
     7741 attatggatg tggaaaatca cctgtatgcg cgcaaagaca ttaaacagct gggcattgcg
     7801 ccgatgacca gtatgttcag ctgcggtact aatgaacgtc ggatgtgcga caccattcat
     7861 ccgcaaattc atgactctga tcgtctgtcc atgtggcggg gcaacggcga gtggatttgc
     7921 cgtccgctga acaatccgca aaaattgcag ttcaatgctt acaccgacaa caacccgaaa
     7981 gggtttggtt tattgcaact ggatcgtgac ttctcccatt atcaggacat tatgggctgg
     8041 tataacaaac gcccaagttt gtgggtggaa ccgcgtaaca agtggggtaa gggcaccatc
     8101 ggcctgatgg aaatcccaac aacgggcgaa acgctggata acattgtctg cttctggcag
     8161 ccagaaaaag ctgtaaaagc aggtgatgag tttgcattcc agtatcgtct gtactggagt
     8221 gcgcaaccgc ctgttcattg cccattagcg cgcgttatgg cgacgcgtac cggcatgggc
     8281 ggtttcccgg aaggttgggc gccaggtgaa cactatcccg aaaaatgggc gcgtcgtttt
     8341 gccgtcgatt tcgttggtgg tgatctgaaa gctgccgcgc caaaaggcat tgagccggtg
     8401 attacgcttt ccagtgggga agcgaagcaa atcgaaattc tctatattga acccatcgat
     8461 ggttatcgta ttcagtttga ctggtatccg acttcggact ctactgatcc ggtcgatatg
     8521 cggatgtatc tacgttgtca gggggacgct atcagtgaaa catggctgta tcagtatttc
     8581 ccgccagcgc cggataaacg tcagtatgtt gacgaccgcg tgatgagtta atcgtttttt
     8641 cttcggcacc ttcttcggga ggtgccgtct ggttaaacac gatcccgctc gcatttttcc
     8701 ctaagttaaa tgagtaatct gatggtgtgt atttcagata caccttgtca gccactaaca
     8761 gggagtgcgt atgtttccag aataccgaga tttaatatcc cgtctgaaaa acgaaaatcc
     8821 tcgctttatg tccttgttcg ataaacacaa taaacttgat catgaaattg ccagaaagga
     8881 aggttccgac ggtcgagggt acaatgcgga agtggtccgc atgaaaaaac aaaagctaca
     8941 gttaaaagat gagatgctca aaatcctgca gcaggagagc gtcaaagagg tgtaaacttt
     9001 cctaagccgc cagccaggcg gcttttttaa caacagcatg gattgactgg agataagatg
     9061 actgaaacga taaaagtaag cgaatcactt gaattacatg ctgttgcgga aagtcacgtc
     9121 acacctcttt atcagttaat ctgtaaaaat aaaacctggt tacagcagtc gctaaactgg
     9181 ccgcagttcg ttcagagtga agaggacacg cgaaaaacgg tgcagggtaa tgtgatgttg
     9241 catcaacgcg gctatgccaa aatgttcatg attttcaaag aagatgaact tatcggcgtt
     9301 atctcgttta atcgtattga accactgaat aaaactgctg aaatcggcta ctggctggac
     9361 gaatctcatc aggggcaggg gatcatttct caggcgctgc aggcattgat tcatcattac
     9421 gctcagtctg gtgaacttag acgcttcgtg atcaaatgtc gggtggacaa tccgcaaagc
     9481 aaccaggtcg ctttgcgcaa tggttttatc cttgaaggtt gcctgaaaca ggctgagttc
     9541 ctgaatgatg cctatgatga tgtgaactta tacgcgcgta ttatcgattc ataataaccc
     9601 tgccagcggc gttcgcgtaa tgcgctcctc gccgttaatg acttttggac cacggacatg
     9661 aaccgtgtcg ccatcaaagg cttcaacgac ggcgtggtcg gttaactcca catggttttc
     9721 aattatcacc gcacctgaaa tacggctctc accttgtatt acaacgtgct cgtcgagcag
     9781 aatcggtcca ccacgtacca cggcatttcc gccaatcaag acatgatgtt ttaacacaca
     9841 attaccttcc acaatggcat attccgccac ctgcgaactg taatgaatcg tcggaatggc
     9901 atcttcttct atgccagctt tcacctgcgc gtgaccgtag actttagcgc aatcgcatag
     9961 ccagacgttg ttctcttcat taccttcaac actggcaaaa tcaaaaactt cggcgcgatg
    10021 ttcaataaaa gcataccgga cgacggcatc gccataaatt tgtgcctggt ggacaatacg
    10081 cgaggcgcta acccttgcgc gatcataaat ttggagcagg agttgatggt cgggcgttaa
    10141 gccttgtgcg gcgacgatca tagaatgttg atcaattaag gcgtgaccaa atattctaca
    10201 ttgcccgtaa actaatgagt cacgaatggt gacactgtca cttatatatg caccctggct
    10261 gatttcacta ttatcgatcc agacgttatc cgttgcgtaa acctctcccc acagcacgct
    10321 ggtgccggtg atacgcgtat taccagaaat caccgttccg ccgaacgcaa tagcattctg
    10381 gtcgtaaatc cagcaatttc cttcctgtgc taacaccgtt tcgcgatcga tccagccgcc
    10441 agcggttcct gctatcacat cgttaaaatc gctaatggcg ataatctgcc gtaataacac
    10501 acttttttta gtgccatctt cctgataact aaaggctcgc tgttcttcac ttagacgata
    10561 tttgcgcata gcgttttctc acaggtgact tactataacc gtagcaaatt ctgcggctct
    10621 ggctatgctc gagaaaattc cataaaatgc atttcaaata tactttataa attaaacaaa
    10681 atgagtaaga agatgcagag cgataaagtg ctcaatttgc cggcaggcta ctttggtatt
    10741 gtgttgggga cgatagggat gggatttgcc tggcgctatg ccagccaggt ttggcaggtc
    10801 agccactggt taggggatgg gctggtgatt ctggcgatga tcatctgggg attattaact
    10861 agcgcattta ttacccgact catacgcttt ccgcatagcg tgctggcgga agttcgccat
    10921 ccagtgctga gcagttttgt gagtttgttt ccggcaacga cgatgctggt ggcgattggt
    10981 tttgttccgt ggtttcgccc actggcggtg tgcctgttca gttttggtgt cgtggttcag
    11041 ttggcttatg ccgcctggca aactgcggga ttatggcgcg gatctcaccc tgaagaagct
    11101 accacgcctg gactgtatct gccgacagtt gccaacaact ttatcagcgc aatggcctgt
    11161 ggtgcgttgg gctacaccga cgccggtctg gtgtttttag gcgcaggcgt tttctcatgg
    11221 ctaagtcttg aaccggtgat cttgcagcgt ctgcgcagtt cgggagaatt acccacggca
    11281 ctgcggacat cactcggcat tcagctcgct cctgcgctgg tggcctgtag tgcctggttg
    11341 agcgtcaacg gcggcgaggg tgatacgctg gcgaaaatgc tttttggtta tggactgctg
    11401 caactgctgt ttatgctacg tctgatgcca tggtatctct cccagccatt taatgcttca
    11461 ttctggagtt tctcgttcgg cgtatctgca ctggcaacca ccggtttgca tctggggagt
    11521 ggcagcgaaa atggattttt ccatacgctg gcggtaccgc tgtttatctt taccaatttt
    11581 attattgcaa tactgctcat ccgtactttt gcgcttctga tgcagggaaa attgttagtc
    11641 agaaccgagc gcgccgtttt aatgaaagca gaggacaaag aatgatcatt cgtgacgaaa
    11701 actattttac tgataaatat gaattaaccc gcacacactc tgaagtactg gaagcggtga
    11761 aagtggttaa accgggtaaa acgctggatc tgggctgtgg caatggtcgt aacagtcttt
    11821 acctggcagc caatggttat gatgttgacg catgggataa aaatgccatg agtatcgcca
    11881 atgtagagcg cattaaatcc attgaaaatc tggataattt acacacccga gtcgttgatc
    11941 tgaataacct cacatttgat agacagtacg attttattct ttcgactgtg gtactgatgt
    12001 tccttgaggc taaaaccatc ccagggctga ttgccaatat gcaacgttgc actaaacctg
    12061 gtggttacaa cctgattgtg gcggcgatgg ataccgctga ttatccatgt accgtcggct
    12121 tcccgtttgc cttcaaagag ggagaattac gtcgatatta cgaaggctgg gagagggtga
    12181 aatacaatga agacgtcggc gagctgcacc gcaccgacgc caacggtaat cgtattaaac
    12241 tgcgtttcgc cacgatgctg gcacgtaaaa aatgacccgc taagaataag gcgcgtgaaa
    12301 attccccacg ctgagatgat ttactgttct tcttttcggt aagcatattt tttatcgaag
    12361 ggatgtgaaa ttaatcacag tagtcgaagt tttttagcag cttaacttac tgaaatttaa
    12421 ctactgatga ttgacttagc ccctttttcg gcattgacta tgtcgtctga aaaggggctg
    12481 aaaaatttat tttcaccaac actttttttg ccacaacacg aagcggcgct ttttgctata
    12541 acttaaaaag taatataatc atc
//
LOCUS       DAZPEP010000117        12446 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZPEP010000117 DAZPEP010000000
VERSION     DAZPEP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.117"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..398)
                     /locus_tag="ACHUT9_003135"
     CDS             complement(<1..398)
                     /locus_tag="ACHUT9_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349548.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINENFVGMDFTCEFRPEKMTNLINIKNR"
     gene            complement(687..950)
                     /gene="cedA"
                     /locus_tag="ACHUT9_003136"
     CDS             complement(687..950)
                     /gene="cedA"
                     /locus_tag="ACHUT9_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFR1349549.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            1133..3394
                     /gene="katE"
                     /locus_tag="ACHUT9_003137"
     CDS             1133..3394
                     /gene="katE"
                     /locus_tag="ACHUT9_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFR1349550.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3652..4410)
                     /gene="chbG"
                     /locus_tag="ACHUT9_003138"
     CDS             complement(3652..4410)
                     /gene="chbG"
                     /locus_tag="ACHUT9_003138"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFR1349551.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(4423..5775)
                     /gene="celF"
                     /locus_tag="ACHUT9_003139"
                     /gene_synonym="chbF"
     CDS             complement(4423..5775)
                     /gene="celF"
                     /locus_tag="ACHUT9_003139"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFR1349552.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5880..6722)
                     /gene="chbR"
                     /locus_tag="ACHUT9_003140"
     CDS             complement(5880..6722)
                     /gene="chbR"
                     /locus_tag="ACHUT9_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFR1349553.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6730..7080)
                     /gene="chbA"
                     /locus_tag="ACHUT9_003141"
     CDS             complement(6730..7080)
                     /gene="chbA"
                     /locus_tag="ACHUT9_003141"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFR1349554.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(7131..8489)
                     /gene="chbC"
                     /locus_tag="ACHUT9_003142"
     CDS             complement(7131..8489)
                     /gene="chbC"
                     /locus_tag="ACHUT9_003142"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFR1349555.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(8574..8894)
                     /gene="chbB"
                     /locus_tag="ACHUT9_003143"
     CDS             complement(8574..8894)
                     /gene="chbB"
                     /locus_tag="ACHUT9_003143"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFR1349556.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(9193..9531)
                     /gene="osmE"
                     /locus_tag="ACHUT9_003144"
     CDS             complement(9193..9531)
                     /gene="osmE"
                     /locus_tag="ACHUT9_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFR1349557.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9733..10560
                     /gene="nadE"
                     /locus_tag="ACHUT9_003145"
     CDS             9733..10560
                     /gene="nadE"
                     /locus_tag="ACHUT9_003145"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFR1349558.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREADIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10790..11677
                     /gene="cho"
                     /locus_tag="ACHUT9_003146"
     CDS             10790..11677
                     /gene="cho"
                     /locus_tag="ACHUT9_003146"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFR1349559.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11637..12212)
                     /gene="ves"
                     /locus_tag="ACHUT9_003147"
     CDS             complement(11637..12212)
                     /gene="ves"
                     /locus_tag="ACHUT9_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFR1349560.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         3345 a         3156 c         2789 g         3156 t
ORIGIN      
        1 aacctatttt ttatattgat caaattcgtc attttttcgg gtcggaattc acaggtaaaa
       61 tccatcccaa caaaattctc attaatatct cttatccgaa actcactgcc ataaagatta
      121 ttaattatca ctctatattt aacattgtcc tgcagatacg tatcaaaact aagtgctgga
      181 gctcctggct ttgaggcatc atagttaatg ttagacatat aggcaatcat acgattgaag
      241 ttcgactctg ctatttcatc gcagtcacat tgacccgttg atataaagaa agtgagtgcc
      301 gccatggtag actcaagcct ttctttaaaa gagtcgaaag ccaggctttt aatatttaaa
      361 tcaccataat tactctgtat taagtttgta gaaaacatct cccgccccat attgttaaca
      421 aaattattat ctcatttaaa tctaagtcac ttacaatata agtttaagag cgacgccaca
      481 ggatgaacta tcaaaaatag ctcatcatga ttagcaaaac ttaaccattt taaaataaat
      541 aaacaattaa agaaaaaaga tcacttattt atagcaatag ctcgtcaaag gcagcttttt
      601 gttacaggtg gtttgaatga atgtagcaac gaaatgcaga atttcaggtc atgtaactcc
      661 cggcaaaacc gggaagtatg taatccttac tcagtcactt ccccttcctg gcggatctga
      721 tttgcccaac gttgggcaga ttcaggcaca gtaaacgccg gtgagcgcag aaatgactct
      781 cccatcagta caaacgcaac atatttgcca cgcagcatcc agacatcacg aaacgaatcc
      841 atctttatcg catgttctgg cggcgcgggt tccgtgcgtg ggacatagct aataatctgg
      901 cggttttgct ggcggagcgg tttcttcatt actggcttca ctaaacgcat attaaaaatt
      961 agaaaaactg tagtttagcc gatttagccc ctgtacgtcc cgctttgcgt gtatttcata
     1021 acaccgtttc cagaatagtc tccgaagcgg gatctggctg gtggtctata gttagagagt
     1081 tttttgacca aaacagcggc cctttcagta ataaattaag gagacgagtt caatgtcgca
     1141 acataacgaa aagaacctac atcagcacca gtcaccacta cacgattcca gcgaagcgaa
     1201 accggggatg gactcactgg cacctgagga cggatctcat cgtccagcgg ctgaaccaac
     1261 accgccaggt gcacaaccta ccgcccctgg gagcctgaaa gcccctgata cacgtaacga
     1321 aaaacttaat tctctggaag acgtacgcaa aggcagtgaa aattatgcgc tgaccactaa
     1381 tcagggcgtg cgcatcgccg atgatcaaaa ctcactgcgt gccggtaacc gtggtccaac
     1441 gctgctggaa gattttattc tgcgcgagaa aatcacccac tttgaccatg agcgcatccc
     1501 ggaacgtatt gttcatgcac gcggatcagc agctcacggt tatttccagc catataaaag
     1561 cttaagcgat atcaccaaag cggatttcct ctcagatccg aacaaaatca ccccagtatt
     1621 tgttaggttc tctaccgttc agggtggcgc tggctctgca gataccgtgc gtgatatccg
     1681 tggttttgcc accaagttct atactgaaga gggtattttt gacctcgttg gcaataacac
     1741 gccaatcttc tttatccagg atgcgcataa attccccgat tttgttcatg cggtaaaacc
     1801 agaaccgcac tgggcaattc cacaagggca aagtgcccac gatactttct gggattatgt
     1861 ttctctgcaa cctgaaactc tgcacaacgt gatgtgggcg atgtcggatc gcggcatccc
     1921 ccgcagttac cgcaccatgg aaggcttcgg tattcacacc ttccgcctga ttaatgccga
     1981 agggaaggca acgtttgtac gtttccactg gaaaccactg gcaggtaaag cctcactcgt
     2041 ttgggatgaa gcacaaaaac tcaccggacg tgacccggac ttccaccgcc gcgagttgtg
     2101 ggaagccatt gaagcaggcg attttccgga atacgaactg ggcttccagt tgattcctga
     2161 agaagatgaa ttcaagttcg acttcgatct tctcgatcca accaaactga tcccggaaga
     2221 actggtgccc gttcagcgtg tcggcaaaat ggtgctcaat cgcaatccgg ataacttctt
     2281 tgccgaaaac gaacaagcag cattccatcc aggtcatatt gttcctggtc tggacttcac
     2341 caacgatccg ctgttgcagg gacgtttgtt ctcctatacc gatacacaaa tcagtcgtct
     2401 tggtgggccg aatttccatg agattccgat taaccgtccg acctgccctt accacaactt
     2461 ccagcgtgat ggcatgcatc gtatggggat cgacactaac ccggcgaatt acgaaccgaa
     2521 ctcgatcaac gataactggc cgcgcgaaac accgccggga ccgaaacgcg gcggttttga
     2581 atcatatcag gaacgcgtgg aaggaaataa agttcgtgag cgcagtccat cgtttggcga
     2641 atattattcc catccgcgtc tgttctggct aagtcagacg ccattcgagc agcgccatat
     2701 tgtcgatggt ttcagttttg agttaagcaa agtcgttcgt ccgtatattc gtgagcgcgt
     2761 tgttgaccag ctggcgcata ttgatctcac tctggcccag gcggtggcga aaaatctcgg
     2821 tatcgaactg actgacgacc agctgaatat caccccacct ccggacgtca acggtctgaa
     2881 aaaggatcca tccttaagtt tgtacgccat tcctgacggt gatgtgaaag gtcgcgtggt
     2941 agcgatttta cttaatgatg aagtgagatc ggcagacctt ctggccattc tcaaggccct
     3001 gaaggccaaa ggcgttcatg ccaaactgct ctactcccga atgggtgaag tgactgcgga
     3061 tgacggtacg gtgttgccta tagccgctac ctttgccggt gcaccttcgc tgacggtcga
     3121 tgcggtcatt gtcccttgcg gcaatatcgc ggatatcgct gacaacggcg atgccaacta
     3181 ctacctgatg gaagcctaca aacaccttaa accgattgcg ctggcgggag acgcgcgcaa
     3241 gtttaaagca acaatcaagg tcgctgacca gggtgaagaa gggattgtgg aagctgacag
     3301 cgctgacggt agttttatgg atgaactgct aacgctgatg gcagcacacc gcgtgtggtc
     3361 acgcattcct aagattgaca aaattcctgc ctgatgggag gcggcgcaat tgcgccgcct
     3421 caaatgattt acatagtgcg ctttgtttat gccggatgcg gcgtgaacgc cttatccggc
     3481 ctacaaaact gtgcaaattc aatatattgc aggaaacacg taggcctgat aagcgaagcg
     3541 catcaggcag ttttgcgttt gtcagcagtc tcaagcggcg cagttacgcc gcctttgtag
     3601 gaattaatcg ccggatgcaa ggttcacgcc gcatctggca aacatcctca cttacacatc
     3661 ccgataactc cccaaccgat aaccacgctg agcgatagca cctttcaacg acgctgatgt
     3721 caacacatcc agctccgtta agcgtgggaa acagtaagca ctctgacgga tagtattatc
     3781 gataaacgcc ggatgacaca tcacctccag cgaacgatca ccccgatggc ttgcgtcatc
     3841 cagcacttgc aggaacagtg cctcgctaat ctcttcacca taaaacgcgc tactgaatcc
     3901 ctgagaactg cgcagattag ccggtaaatc accagagtta gatagcggct gacggtcgat
     3961 gcgcaacgca atcccctctt cagccgcaaa cctggcaaca atcgggaaaa tctgcgggaa
     4021 catatgcaca tgatgatggc tatcaagatg cgtaggtttg cgtccaaata gctcaatgaa
     4081 acgcaaatac tgactggcaa gctcctgagt aatttcttcc agcggtaaag catcttcttc
     4141 tgccaactgc cagatccatt ttcccagcac accatcgcgg gttaaccccg gcatagctgt
     4201 cagtggcttg cccatagtaa ggacaaagta catccctatg gccagactcg gttcatcacg
     4261 actcaactgc accgcatggt caatagcctg cccattcacc agcgccgtcg tcgatgtgac
     4321 aatcccattg cgacaggcct cgataatgcc gtagttctga cctttgctta agccaaaatc
     4381 atcggcatta acaatcagta agcgttccat aatcagcctc ggttaatgtg cttttttaag
     4441 ctctgcgatg cagtcggcaa aatttggcag ccatttctcg tgcgccagaa tcatctcgcg
     4501 tgccagcagc tcagcatcgc gatcggaatg caccaacgga ctaaggttta gcgccagtaa
     4561 cacatcgtta aattctccgc taagtgcggc gttgctggca gcaatctcga agcctttaat
     4621 ggtgtgaatc agccccatca ctttatcatc gaaatgcgta atgcgcggat gtggcgtcgc
     4681 gccatcgcgc cccagcttac aggtcatttc taccgcccag tctgccggaa tattatcaat
     4741 atgcccatga tgcgggatat taacgtaatg ttcagcttgc ttgtcgttgt agatagcgtt
     4801 gatcacttcg cacgctgcat cagagtaata agccccaccg cgctgttcca gttctttcgg
     4861 cttaactttc aactccgggt ttttatacag ctcaaaaagt tgtttctcga ctttctgtac
     4921 tacctgcgct cgtgcgccgc ctttgtagta ttcgcccatt tcaatagcca gcatctcttt
     4981 ttgcttgaag taataaagca aataagaaca cggcaacaga ttcagagaac gaattaagcc
     5041 ttcgctaaat ggcagatcga aaatattttt aacgccagat gcttttaact gcccggatgc
     5101 cacaccatca agcaattcgg caaagcgcga cttgccattt accagcacat ccttaatgaa
     5161 caccatatgg ttgaggccga acaaatcgat agataaatca tcgctgtctt tcagcatcag
     5221 aacatcgtga ataaacatct tcatgccgat cggaatatta cacacgccga taaagcgttt
     5281 aaatccggta tgacgataaa cggcttcagt aaccattccc gccgggttag tgaagttaat
     5341 cacccatgca ttcggacaaa gttcttcgac atcttttacg atgtcaaaaa tcaccggaat
     5401 ggtacgcaga cctttaaaca gaccgcccgc gccgttggtt tcctggccaa gataaccatg
     5461 acttaatgga atacgttcat ccagttcgcg cgccggtaat tggcctacgc gcagttgggt
     5521 agtaacgaaa tcagcatctt tcaatgcttc gcggcgatcc agcgttttat aaagcttcat
     5581 cgggacgcca gcgttatcaa tcatccgttg gcagagttca aaaatgatat ccagtttctc
     5641 tttaccatct tcgacatcca ccagccataa ttcgctgacc ggcaattcgt gataacgctt
     5701 aataaatcct tccagtaact ccggggtata gctgctcccg ccaccaatag tgacgacttt
     5761 taatttctgg ctcataattt ctcccttcag tacagaatac tgatatctgg catatctgcc
     5821 cccccggaca taaataatcc agcaacagga cagatatgtg aattgtcagg tataacgact
     5881 tactgattaa attcagtcaa tttcttacga tagctcttag gcgtaaagga cgttaatttc
     5941 ttaaacgttt taataaacaa actcgggcta ctataaccgg cctcaaacgc aatatccgtc
     6001 acggaatagt tggtcatttc cagttgtttt ttggcaaaat taatacggat ctcattaata
     6061 atctgcattg gtgttttgcc ataatatcgt tgagtcgctc gcgtcaaata ttcctgtgat
     6121 ttggctgaca acgtcaccat attctccagc gccgattcac taaactgctc tttatcatgc
     6181 atcttttcta ccgtactttt cagccactgc ggtacatcat caatcacctg ttcttcgcgg
     6241 taatgacgta aacggttaat gacataaaaa gtaaccatct caacaaactc ttccagccct
     6301 gtttcgcgga aattcaatga agaaatcact gtttccacat aggtgagaaa agcgttattg
     6361 gtccggtata cctgcgaagc gacaaagcaa taaggcaaca acggcaggta atgctgctca
     6421 aaaaagcgtt tactgatccc aacgttcaat atacgcgtgg caccaaactc ataaaaactt
     6481 tgatggtgcg aacctaacgg aataaaaaca aaatcgcccc gttccagtaa cacgcgctta
     6541 ccgttaatct cctggaaata acgcccggtt aataccagag taaattcata atagtcgtgc
     6601 tgatgcagcc cgctgatact ctcagttttg ttatagataa acacatggaa gtttttgcca
     6661 ttaaacaact gctgttctcg ggcagtggca atttccggcg cgttaatcac tggctgcatc
     6721 atcgactcct tatgccttca gtttttcatg aagctcaatt aattcagtaa tcagttcacg
     6781 cgcaagcatg gacgtcatta aatgatcctg agcgtggacc agcaccagac tcactttcat
     6841 cttaccttcg cccgcatcgc cttcaatcag tttcgtctgt accagatgcg cttcattcaa
     6901 tgccatccgt gactgatcca tcatggcttt tgctgcagca aaatcgccct gcttcgcctg
     6961 tttcagtgcc gcatacgcca ggctgcgcgc ttgtccggag ttgatgataa gccccatcac
     7021 cacttcttcc agctcttcag cttccgtttg cgtatcggga atgttatcga gatccatcat
     7081 acatcgttcc tctctattct taccggcacg attacccgta ccggcatcga ttaaaatttc
     7141 agggcgttag cgatatcttc ttcgcgctct tctttatcaa tcgcgttttg tgctttgtta
     7201 gccaccacaa caaagggcag ataaattaac gttgcgatac caaggttgaa gagtgcaacc
     7261 agcaatgcgg cgacgctacc gttggtgtta aagaaggctc ccagaccggt tggcatggtc
     7321 cacggtgcaa tattggtcac cggaggaatg atgcccatgt agtacgctgc gagggtgatt
     7381 gccgccagaa tcggttgtac cagtacaaac gggataaaca tcaccgggtt catgataatt
     7441 ggcagaccaa acagaatcgg ttcgttaatc tggaagatgc cggacggcag cgccagtttt
     7501 gccacctgac gataatcagc acggcgagag gcgataaaga tagccaggat caggcctaaa
     7561 gtcgcaccac tgcccccaag gaaaataaag gagtccagca tcggcttggc ccagatatgg
     7621 aaggtcttac cggctgccag cgccgcttcg acggaaccat attgctgata ggtcgcgata
     7681 ttttccagcg cccacggcgt cataatgccg ttgtccagtg cggtcagcgc cagcgcgcca
     7741 tgaataccga agaaccagag cagtggaaca aagatcacat aggcccagcc caccacgcta
     7801 cccaacgatg ccagtggggt tgagatggta tccataatga tctgatggaa gttggtgccc
     7861 caggtattca acgcccaggc aataatcccc atcacggaaa gaataataaa gccgggaatt
     7921 aatgccgaga aggaacgcga tactgaagca ggtacgctat cgggcaattt aatgacccaa
     7981 ttgcggcgga caataaaggt aaacatttct gccaccacca ggccaataat aatcccggag
     8041 atgatattcg ctccacctaa ccagtttgcg ccaaccacat aggcctcacc gacactatat
     8101 ggggtgacgg tcataaatgc tgcaacggat aacaacccag ccgccagcgc atcgacttta
     8161 cgctcttctg ccagcgccat gccaataaag aacggtgcca ttaaagacat tatccctaat
     8221 gttccgttat atacgttgcc gccaatacct ttcaggccat taagtgtttc aatggttgag
     8281 gcgtcgaggc gaatacctaa ggaataaaaa aacgccccct ccccaaagct tagaaaaacg
     8341 ttgttaatta atacaaacat ggcccccgca agggttaacg gcattaagcg aataaagcca
     8401 tttttgattg cattaacgtg tggctgcttt cctattttaa ctgcaaaagg gaggagtacc
     8461 ttttcaagcg atgcaataac attactcata gaaaaatacc cttaaaaacc gcaatttaaa
     8521 tattgcggta ttgatttatg aaataactct ttgacgggaa aatttaaaat aaattaattt
     8581 gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc atcgactttg
     8641 ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg ctggatttcg
     8701 ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg acctttttca
     8761 ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt ttctgcctgt
     8821 gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa cagataaatg
     8881 tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga cactccgcaa
     8941 gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt tttttgtggc
     9001 tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta taaattgttt
     9061 ctccttgacc tgtggttatg accctcttca catttcgggc aaataattcg cgaagataat
     9121 taagaactca ggccaaaaaa aaccggcgca atggccggtt tccgttgtta ctcaagcttt
     9181 cagacgaatt gattacttcg cagcctgtgg atcagtgtcg tattcagcac aggtctgata
     9241 accggagttg atgacatgtc cggtatcatc taacgcgaca aagtaggttt ctgctttacc
     9301 atcacgttga cccaggatgt aggtctggca agtaccacga gcatggatca tgctcacttc
     9361 agacgaaggt ttacccgcaa tttgtgcaac ctgcgcccgg ctcatgcctt ttttgacgtc
     9421 tttcaccaca ggctgtacaa actggtcttt agtacgatca taagccgtac aacccgccag
     9481 catggttaat accgccgctg cactcagaat tcctgccata ttcttgttca tattccgtcc
     9541 tcttgtttat cagcgtgtta gataagcctg gaatacattg ggcgcttttt caagcccgcg
     9601 aacgaaacgg ctccgctttc agaggattcc tgtatgacgt tttaaccacc attcagcccg
     9661 ctgtcgcttg tcgtttcagt ggcaacgggt tagctttaag gaagttttgt cttttctgtc
     9721 tggaggggtt caatgacatt gcaacaacaa ataataaagg cgctgggcgc aaaaccgcag
     9781 attaatgctg aagaggaaat tcgtcgtagt atcgattttc tgaaaagcta cctgcaaact
     9841 tatccgttca ttaaatcact ggtgctcggg atcagcggcg gtcaggactc cacgcttgcc
     9901 ggaaagctgt gccagatggc gattaatgag ctgcgcctgg aaaccggcaa cgaatcactg
     9961 caatttattg ccgtacgcct gccctatggt gttcaggccg acgaacaaga ttgccaggat
    10021 gccattgcct ttattcaacc ggatcgcgta ttaaccgtta atatcaaggg cgcggtattg
    10081 gccagcgaac aggcattgcg ggaagcagac attgaactga gcgattttgt ccgtggcaat
    10141 gaaaaagcgc gtgagcggat gaaagcacaa tatagcattg cgggtatgac cagcggtgtc
    10201 gtggtgggca ccgatcatgc agcagaagcc attaccggat tcttcactaa atatggtgac
    10261 ggcggtacag acattaatcc gctgtatcgt ctcaacaaac gtcagggtaa acagttactg
    10321 gcggcattag gttgcccgga acacctttat aagaaagcgc caacggccga tctggaagat
    10381 gatcgccctt ctctgccaga tgaagtggca ctcggcgtga cctatgacaa tatcgacgac
    10441 tatctggaag ggaaaaacgt acctgaacag gtcgccagaa caatagagaa ctggtatctg
    10501 aaaaccgaac ataaacgccg tccgccaatt accgttttcg atgatttctg gaaaaagtaa
    10561 taatttgcac ataatggttt gtgcgaaaaa gagtaatttg ttcacgccgg atgcggcgtg
    10621 aacgccttat tcgacctaca aaactatgca aattcaatat attgcaggag tcgaataggc
    10681 ctgacaggcg tagcacgtca gacggtgtaa cctttgtcat cgacccgcct cttttttaat
    10741 cgctccccgc ctgttacact ggatagataa ccagcattcg gagtcaacag tggtacggcg
    10801 tttaacttct ccgcggctcg aatttgaagc tgcggcaatt tatgaatatc ccgaacattt
    10861 acgttcattc cttaatgact tacccacccg accaggggtg tatctgtttc atggcgaaag
    10921 tgacaccatg ccgctctata tcggcaaaag cgttaatatc cgcagtcggg tgctttctca
    10981 tttacgcacc ccggatgaag ccgccatgct acggcaatcc cggcgaatta gctggatctg
    11041 taccgcaggc gaaatcggcg ctctgctcct tgaagcgcga ttaatcaaag aacaacagcc
    11101 gctgtttaat aaacggttgc gccgcaatcg ccagctctgt gccctgcaat taaatgaaaa
    11161 gcgcgtcgat gtggtgtatg ccaaagaggt ggatttttca cgagccccca acctgtttgg
    11221 cctgtttgcc aataggcgcg cagctttgca agcattgcag accatcgctg atgaacaaaa
    11281 actttgttat ggcctgctgg gactggaacc gttaagtcgc ggtcgtgcat gttttcgttc
    11341 agcgctaaaa cgttgcgccg gagcatgctg cggtaaagag agccatgagg aacatgcgct
    11401 acgcttgcgc cagtctctgg agcgtttgcg ggtggtgtgt tggccttggc aaggtgcggt
    11461 ggcactgaaa gaacagcacc cggaaatgac tcaatatcat attattcaaa actggctgtg
    11521 gctgggggcg gttaattcgc tggaagaagc gacaacgtta attcggacac ccgccgggtt
    11581 tgatcacgac ggttataaaa ttctttgtaa gccgctgctt tccggtaact atgaaattac
    11641 tgaacttgat ccggcgaatg accagcgagc cagttgattt cactgaacaa cagtttacct
    11701 tgcggttgta aaagccgcag ggtgtgcctg ccatcgaacc agcacgcgcc ctggtcggta
    11761 gtgagcaatt tatcgcccag ttgccacgcc ccgttaatca caaacaccac cccgccgcgt
    11821 gagccaaagg tagtaaacgt acgttcggca attctgactt tcgccttaca gacatccagg
    11881 cgtgtcatga tgttgaaatc catcgacatc tgcccttccg tcagtttcgc ttttaccacc
    11941 tggtccgctg caaaggcaaa aggctgtaac ggttttaagg tatggttaaa gcggtctgcg
    12001 ctttcaagga gcatctcgcc gccttccagc aacgtcacta tcctttccat gccgggaaac
    12061 aaagaaaact ccccattcgc cgcgatggat gcaatgctgg cacgccagta aaaatcacgt
    12121 tttgccggtg ggaaagtgca aatttcgcgc gtttcaccgg cagcatttcg ccacagattc
    12181 accgacattt tacgcatatc aaagtattcc atactcgctc ccggcccgtt cttttcattg
    12241 ttatcgttca ggtaaccgtg gatggttatc ggcaactcgg gtgatttacg ttagtggtga
    12301 tcaggctttt tttacaactg tttgagaaga gaaaagaaaa ccgccgatcc tgtccaccgc
    12361 attactgcaa ggtagtggac aagaccggcg gtcttaagtt ttttggctga aagattattc
    12421 agcagttgca ggcattttac cttttg
//
LOCUS       DAZPEP010000118        12413 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZPEP010000118 DAZPEP010000000
VERSION     DAZPEP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.118"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..775
                     /locus_tag="ACHUT9_003148"
                     /pseudo
     CDS             1..775
                     /locus_tag="ACHUT9_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
     gene            1098..2486
                     /gene="narU"
                     /locus_tag="ACHUT9_003149"
     CDS             1098..2486
                     /gene="narU"
                     /locus_tag="ACHUT9_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFR1349561.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2568..6308
                     /gene="narZ"
                     /locus_tag="ACHUT9_003150"
     CDS             2568..6308
                     /gene="narZ"
                     /locus_tag="ACHUT9_003150"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFR1349562.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            6305..7849
                     /gene="narH"
                     /locus_tag="ACHUT9_003151"
     CDS             6305..7849
                     /gene="narH"
                     /locus_tag="ACHUT9_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFR1349563.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7849..8544
                     /gene="narW"
                     /locus_tag="ACHUT9_003152"
     CDS             7849..8544
                     /gene="narW"
                     /locus_tag="ACHUT9_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFR1349564.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            8541..9221
                     /gene="narI"
                     /locus_tag="ACHUT9_003153"
     CDS             8541..9221
                     /gene="narI"
                     /locus_tag="ACHUT9_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFR1349565.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            9300..10193
                     /locus_tag="ACHUT9_003154"
     CDS             9300..10193
                     /locus_tag="ACHUT9_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFR1349566.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(10289..11134)
                     /gene="nhoA"
                     /locus_tag="ACHUT9_003155"
     CDS             complement(10289..11134)
                     /gene="nhoA"
                     /locus_tag="ACHUT9_003155"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFR1349567.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            11307..11876
                     /locus_tag="ACHUT9_003156"
     CDS             11307..11876
                     /locus_tag="ACHUT9_003156"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFR1349568.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11880..12107)
                     /gene="pptA"
                     /locus_tag="ACHUT9_003157"
     CDS             complement(11880..12107)
                     /gene="pptA"
                     /locus_tag="ACHUT9_003157"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFR1349569.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(12207..>12413)
                     /locus_tag="ACHUT9_003158"
     CDS             complement(12207..>12413)
                     /locus_tag="ACHUT9_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1349570.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMGRNYLASVLTGSGLS"
BASE COUNT         3006 a         3162 c         3166 g         3079 t
ORIGIN      
        1 tttgtgcaat tagaatcttg cgaatgtctg caatacctga atctcagcca taatcaatta
       61 tctgatattg ttgcaggtaa taaaaattaa ctcttactgc tggatctatc ccataataaa
      121 ctaacaagtt tacacaatgt cttatttccc aacttgaata cgttacttat taacaaaaac
      181 ctgctttctg aaattaaaat attctatagc aacttctgca atgttcagac attaaacgct
      241 gctaacaacc agttgaaaaa tataaatctt gatttcctga cttgtcttcc atctattaaa
      301 agtttaagac tggacaacaa taaaataacc catattgata ctaataatac atccgatatt
      361 ggaactttat ttcccataat aaaacagagc aaaaacttaa attttttaaa tgtttctggg
      421 aagaacaatt gccctactat gcagctcatg ttatttaatt tgttttcccc aacacttaag
      481 cttaatactg gcctggcaat tctttcgcct ggtgcatttg aagttcactc tgacggaata
      541 gatgtggata acgaattgtt tcactatcct attaaaaata gcatataccc catataatat
      601 acacacttac aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac
      661 cttagatgaa ataaacaata cttactgtaa taacgattat tacaatcagg caataagaga
      721 ggaaccgata gaccttctgg acagatcgtt ttcctccagt tcatggcctt tttagtgaat
      781 aaatcatcac taattctata agtgtctggt cttgttgata atatagttaa tatgtaaccc
      841 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta accccgtttg
      901 aaggttagct tgttagttac atttagtaac acatatctgg ctacttaatc cccattctgc
      961 ccccgcggtt acgctttctt atctattctt tcaatcagca cttcacccaa ttcatcctta
     1021 attctaataa ctggctattg cgccttacct cctgttttgt cttttttcat taccaatgtg
     1081 tgcatgtgag gaacaatatg gcactgcaaa atgagaaaaa tagtcgttat cttttacgcg
     1141 actggaaacc agaaaatccg gccttctggg aaaataaagg aaaacatatt gctcgaagaa
     1201 acctctggat atcagtcagt tgtctacttc ttgccttctg tgtctggatg ctatttagcg
     1261 cagttaccgt taatctcaat aaaatcggtt ttaattttac taccgatcaa ctctttttat
     1321 taaccgcatt accctccgtt tctggcgcat tattgcgtgt tccctactcc tttatggtgc
     1381 ctatattcgg tggacgccga tggacggttt ttagtactgc aatcctgatt attccttgcg
     1441 tctggctcgg aattgccgtg caaaatccga atactccttt tgggatattt atcgttatcg
     1501 ctttgctatg cggttttgca ggtgcaaact ttgcttcgag catgggcaat atcagtttct
     1561 tctttccaaa agccaaacaa gggagcgctc ttgggattaa tggcggatta ggaaacttag
     1621 gtgtaagtgt aatgcagctg gttgcaccgc tggtcatttt tgtacctgta tttgcctttc
     1681 tcggcgtcaa tggcgtaccg caggccgacg gttcggtgat gtcgctggcg aatgccgcat
     1741 ggatttgggt accgctactg gcgattgcca cgatcgccgc ctggtcaggg atgaatgata
     1801 tcgccagttc acgcgcctca attgacgacc agctccctgt cttacaacgc ctgcatctct
     1861 ggctgctgag cctgctttac cttgccacct tcggttcgtt tatcggtttt tctgcgggtt
     1921 ttgccatgct ggcaaaaacc cagttcccgg atgtgaatat tctgcgcctg gcgttctttg
     1981 gcccatttat cggtgccatc gcgcggtcgg ttggtggtgc tatttccgat aaattcggcg
     2041 gcgtgcgggt gacgttgatc aactttattt ttatggcgat tttcagtgcc ctgctgttcc
     2101 ttaccttacc gggcacaggc tccggtaatt tcatcgcctt ttacgccgta tttatggggc
     2161 tgtttctgac tgcgggtctg ggaagtggtt ctactttcca gatgatcgcc gtcatctttc
     2221 gccagataac catttatcgg gtgaagatga aaggcggtag tgatgagcaa gctcagaaag
     2281 aagccatcac cgaaacggcg gcggctctgg gctttatctc agccattggc gcagtgggcg
     2341 gcttttttat tccgcaggcg tttggcatgt cgctcaatat gaccggctct ccggtcggcg
     2401 cgatgaaagt gtttttaatc ttctacatcg tttgtgtgct gctgacctgg ctggtttatg
     2461 gtcggcggaa gttcagccaa aaataagaga tcgatgcctg atgtacaacc gtgtcgcatc
     2521 cgacaaggca tcagatccca attcattcct ggagcaggag tcatgtcatg agtaaacttt
     2581 tggatcgctt tcgctacttc aaacaaaagg gcgaaacctt tgccgatggt cacggacagg
     2641 tgatgcatag caaccgcgac tgggaggaca gctatcgcca gcgttggcag ttcgacaaaa
     2701 tcgtgcgttc cacccacggt gttaactgta caggctcctg tagctggaaa atctacgtta
     2761 aaaatggtct ggtgacctgg gaaatccaac agaccgacta cccgcgcact cgccctgacc
     2821 agcccaatca tgaacctcgc ggctgcccgc gtggcgcaag ttactcctgg tatctctaca
     2881 gcgctaaccg cctgaaatac ccgctcattc gtaaacgact gattgaactg tggcgtgaag
     2941 ctctcaagca acacagcgat ccggtactgg cgtgggcatc gattatgaac gatccgcaaa
     3001 agtgcttaag ctacaaacaa gtgcgtgggc gcggcgggtt tatccgctcc aactggcagg
     3061 aacttaacca actgattgcc gccgctaacg tctggaccat caaaatctat ggtccggatc
     3121 gcgttgccgg tttctcgccg atcccggcga tgtcgatggt gtcatacgct gccggaacgc
     3181 gttatctgtc gctgttaggc ggcacctgtt taagcttcta cgactggtat tgcgacctgc
     3241 cgcccgcctc gccgatgacc tggggcgaac aaaccgacgt gccggaatct gccgactggt
     3301 ataactccag ttacatcatc gcctgggggt ctaacgtacc gcagacacgt acgccggacg
     3361 cccacttctt taccgaagta cgttacaaag gcaccaaaac tatcgccatt acccctgact
     3421 actctgaagt ggccaaattg tgcgaccagt ggctggcacc gaaacaaggc actgatagcg
     3481 ccctggcgat ggcaatgggc catgtgattt taaaagagtt tcatctcgat aatcccagcg
     3541 actactttat caactactgc cgccgctata gcgacatgcc gatgctggta atgctggaac
     3601 ctcgcgacga tggtagctac gtacccgggc gcatgatccg cgcgtctgac ctggtagatg
     3661 gactaggcga aagcaataat ccgcagtgga aaaccgtggc ggttaatacc gcaggtgaat
     3721 tggtagtgcc gaatggttcg attggtttcc gctggggaga aaaaggcaaa tggaatctgg
     3781 aatccattgc cgccggtaag gaaaccgaat tgtcgttaac cctgctcggt caacatgacg
     3841 ctgttgcagg cgtggccttc ccctactttg gcggcattga aaatccgcat tttcgcagcg
     3901 taaaacacaa tccggtgctg gtgcgccaat tgcccgttaa aaacctgacg ttagtcgatg
     3961 gcaacacctg tccggtggtc agcgtttatg atttggtgct ggcgaattac ggcctcgatc
     4021 gcgggctgga agatgaaaac agcgcgaaag attacgctga aatcaaaccg tacaccccag
     4081 cctggggtga gcaaattacc ggcgtaccgc gccagtatat tgaaaccatc gcccgcgaat
     4141 ttgccgatac cgcccataaa acgcatggac gctcaatgat tatcctcggc gctggcgtca
     4201 accactggta tcacatggat atgaactacc gtgggatgat caatatgctg atcttctgcg
     4261 gttgtgtcgg gcaaagcggc ggcggctggg cgcactatgt cggtcaggaa aaactgcgcc
     4321 cacaaaccgg ctggttgccg ctggcctttg cgctcgactg gaaccgccca ccgcgccaga
     4381 tgaacagtac ctcgtttttc tacaatcatt ccagccaatg gcgctatgaa aaagtctctg
     4441 ctcaggagtt actttcaccg cttgccgatg ccagtaagta cagcggtcat ctgattgatt
     4501 tcaacgttcg cgccgaacgc atgggctggc taccttctgc gccgcagctg gggcgtaacc
     4561 cgctcgggat taaagctgaa gccgacaagg ccggattatc ccccacagaa tttaccgccc
     4621 aggcgctgaa atcgggcgat ttacgtatgg cctgcgaaca accagatagc ggcagcaatc
     4681 atccgcgtaa tttgtttgtc tggcgctcta acctgcttgg ctcctccggc aaaggccacg
     4741 agtatatgca gaagtatctg ctggggaccg aaagcgggat tcagggcgag gaactcggtg
     4801 ccagcgacgg gatcaaaccg gaagaagtcg agtggcaaac tgcggcgatt gaaggcaagc
     4861 tcgacctgct ggtgacgctc gacttccgca tgtccagtac ctgcctgttc tccgatatcg
     4921 ttctgcccac cgccacctgg tacgaaaaag acgatatgaa cacctcggat atgcatccgt
     4981 ttattcatcc gctttctgcg gcggtcgatc cggcgtggga atcacgcagc gactgggaaa
     5041 tctacaaagg tatcgccaaa gcattttcgc aagtgtgcgt gggtcatctt ggcaaagaaa
     5101 ccgacgtggt attacaaccc ctactgcatg actctccggc agagctctca cagccgtgtg
     5161 aagtgctcga ctggcgcaaa ggcgaatgcg atctgattcc aggcaaaacc gcgccgaata
     5221 ttgtggcggt ggagcgcgac taccctgcta cgtatgaacg ctttacctcg ctcgggccat
     5281 tgatggacaa acttggcaat ggtggtaaag ggatttcgtg gaatacccag gatgaaatcg
     5341 atttcctcgg caaactcaat tacaccaagc gtgatggccc ggcacagggg cgtccgctga
     5401 ttgacaccgc cattgacgct tcagaagtga ttctggcact ggcaccggaa accaacggtc
     5461 atgttgcagt caaagcgtgg caggcgctgg gcgagatcac cgggcgcgaa catacccatc
     5521 tggcgctgca caaagaggac gagaagattc gctttcgcga tattcaggcg cagccgcgta
     5581 aaattatctc cagccccaca tggtccggtc tggaaagcga tcacgtctcc tataatgcgg
     5641 gatacaccaa cgttcatgag ttaattccgt ggcgcacgct gtcgggacgc cagcagctct
     5701 atcaggatca tccgtggatg cgtgcttttg gtgaaagcct ggtggcttat cgcccgccta
     5761 tcgacacccg tagcgtcagt gagatgcgcc agatcccgcc aaacggcttc ccggaaaaag
     5821 cacttaactt cctgacgccg caccagaaat ggggcattca ctcaacctac agtgaaaacc
     5881 tgctaatgct gacgctctct cgcggtggac cgattgtctg gatcagcgaa accgatgccc
     5941 gagaactgac cattgtcgat aacgactggg tggaagtctt taacgccaac ggcgcgctga
     6001 cggctcgtgc ggtggtcagc caacgtgtac cgccgggtat gaccatgatg tatcacgccc
     6061 aggaacgcat tatgaatatt cctggttcgg aagtaactgg catgcgcggc ggcattcata
     6121 actcggtcac ccgtgtttgc ccgaaaccga cgcatatgat tggcggttac gcgcagctgg
     6181 cctggggctt taactactac ggcaccgtcg gctcgaaccg cgacgaattc atcatgatcc
     6241 gcaagatgaa gaacgttaac tggctggatg atgaaggtcg cgatcaggta caggaggcga
     6301 aaaaatgaaa atacgttcgc aagtcggcat ggtgcttaac ctcgacaaat gtatcggttg
     6361 ccatacctgt tcggtgacct gtaaaaacgt ctggaccgga cgcgaaggca tggagtacgc
     6421 atggtttaac aacgtcgaaa ccaaaccggg cattggttat ccgaaaaact gggaagatca
     6481 ggaagagtgg caaggcggct gggtccgcga tgtgaatggc aagatacgcc cgcgtctggg
     6541 cagcaagatg ggcgtaataa ccaaaatctt cgccaacccg gtggtgccgc agattgatga
     6601 ttactacgaa cctttcacct tcgactacga acatttgcat agcgcaccgg aaggcaaaca
     6661 tatccctact gctcgcccgc gttcgctgat tgacggtaag cggatggaca aagtgatctg
     6721 ggggccaaac tgggaagaac tgctgggcgg cgagtttgaa aaacgtgccc gcgaccgcaa
     6781 cttcgaggcc atgcaaaagg agatgtacgg gcagtttaaa aacaccttca tgatgtacct
     6841 gccgcgcctg tgcgaacact gcctcaatcc cagttgcgtg gcgacctgcc caagcggcgc
     6901 tatctacaaa cgcgaagaag acggcattgt gctgattgat caggataaat gccgtggctg
     6961 gcgtttgtgc attagcggtt gtccgtacaa aaaaatctac ttcaactgga aaagcggcaa
     7021 gtcagaaaaa tgtatcttct gttatccgcg aattgagtca ggccaaccca ccgtgtgctc
     7081 agaaacctgc gtgggtcgca tccggtatct gggcgtgctg ctttacgacg ccgaccgcat
     7141 tgaggaagcg gcgagcaccg agcgcgaagt tgacctctat gaacgtcagt gcgaagtgtt
     7201 cctcgatcca cacgatccct cagtgatcga ggaagccctg aaacaaggta ttccacaaaa
     7261 cgtgattgaa gctgcccagc gttcgcctgt ctacaaaatg gcgatggact ggaaactggc
     7321 gctaccgctg caccctgaat atcgcaccct gccaatggtc tggtacgttc ctccgctgtc
     7381 gccgattcag tcctacgcag atgcgggcgg tttgccgaaa agcgaaggcg tgctgcccgc
     7441 catcgaaagc ctgcgtattc cggtgcaata tctcgccaat atgttgagtg ccggcgatac
     7501 cggtccggta ctgcgggcgc tgaaacggat gatggcgatg cgccactata tgcgttcaca
     7561 aaccgtggaa ggcgttactg atactcgtgc catcgacgaa gtaggcctga gcgtcgccca
     7621 ggtcgaagaa atgtatcgct atctcgccat tgccaactat gaagatcgtt ttgttatccc
     7681 gaccagccat cgggaaatgg cgggcgatgc cttcgcagaa cgcaacggct gcggttttac
     7741 ctttggcgac ggttgccacg gttcggacag taaattcaac ctgttcaaca gtagccgtat
     7801 cgatgccatc aacatcaccg aagtgcgcga caaagcggag ggcgaataat gcagatcctc
     7861 aaagtgatcg gcctgttgat ggagtatccg gatgagctgt tgtgggaatg caaggaggac
     7921 gcgctggcgt tgatccgccg cgacgcgccg atgcttacgg atttcaccca caacctgctt
     7981 aacgcgccgc tgctggataa acaggccgaa tggtgcgaag tgtttgaccg cgggcgcacc
     8041 acgtcgctgc tgctgttcga acatgttcat gccgagtccc gcgatcgcgg ccaggcaatg
     8101 gtggacctgc tggcggagta tgaaaaggtc ggcctgcagc tggattgtcg ggaactgccc
     8161 gattatctac cgctgtatct ggagtattta agcgtactgc ctgacgatca ggcgaaagaa
     8221 ggattgctca atgttgcgcc gatcctcgcc ctgcttggcg gtcgcttaaa acaacgcgag
     8281 gcaccgtggt acgcgttgtt tgatgctctg ctgcaactgg cgggaagtat actttcaagt
     8341 gacagtgtca caaaacaagt aaacagcgaa gagcgcgatg acacccgcca ggcgcttgat
     8401 gcagtgtggg aagaggaaca ggtgaagttt attgaagata acgccacggc gtgtgacagc
     8461 tcgccgctta atcaatatca gcgacgcttt agccaggatg tcgcgccgca gtatgtcgac
     8521 atcagtgcgg gaggtgggaa atgattcagt atctgaacgt ctttttttac gatatctacc
     8581 cgtacatttg tgcgacggtg tttttcctcg gcagctggct gcgctacgac tacgggcagt
     8641 acacctggcg cgcctcctca agtcaaatgc tcgataaacg cgggatggtg atatggtcga
     8701 atctgttcca tatcggcatt ttggggattt tcttcgggca cttgttcggc atgttaacgc
     8761 cgcactggat gtacgcgtgg tttttgccag tggcagcgaa acaactcatg gcgatggtgc
     8821 tcggcggtat ttgcggtgtg ttgacgttga ttggcggcgc aggactgctg tggcgcagtc
     8881 tgacaaatca gcgcgtgcgt gccacttcca ctacgccgga tatcatcatc atgagcattt
     8941 tgctgatcca gtgtctgttg gggctaagta ccataccgtt ttcagcacag tatcctgatg
     9001 gtagcgaaat gatgaagctg gtgggttggg cgcaaagcat tgtcactttc cgtggtggat
     9061 cgtcagagat gctcaacggc gtagcgttcg tcttccgcct gcatctggtg ttgggaatga
     9121 ccatcttcct gctcttcccg ttcacccgac tggtgcatgt gtggagcgcg ccgtttgagt
     9181 actttactcg tcgatatcaa attgtgcgtt cgcggcgata atttcaccct ggccggatag
     9241 cccttcgctc tccggccact tgcaggctat gatagttctc cagtcacagg agaacgttaa
     9301 tgaaaccgca ggtgtaccac gtcgatgctt ttacctctca accatttcgc ggcaattctg
     9361 ccggtgttgt tttccccgcc gataatctta gcgaagcaca aatgcagctt atcgcccgcg
     9421 agttaggcca ttcggaaact gcttttctgc tgcacagcga cgacagcgat gtgcgcatcc
     9481 gctactttac gccaacggtt gaagtgccga tttgcggtca cgcgacagta gctgcgcact
     9541 atgtacgtgc gaaggtgtta ggtttaggaa attgcacgat ctggcaaaca tcgctggcag
     9601 gaaaacatcg cgtgactatc gaaaagcaca acgatgatta tcgtatttcg ctggaacaag
     9661 gtacgccggg ctttgaactg ccgctggagg gtgaagtacg cgcggcgatt atcaacgcat
     9721 tgcatcttac tgaagacgat attctgccgg gtttgccgat ccaggtcgcg acgaccgggc
     9781 attctaaagt gatgatccca ttgaagccgg aagtggatat cgacgctctt tcgcctgacc
     9841 ttaatgcgct gaccgctatc agtaaacaga ttggctgcaa tggtttcttc ccgttccaga
     9901 tccgtccagg caaaaacgaa accgatggtc gtatgttctc gcctgcgatt ggtattgtgg
     9961 aagatccggt gactggcaat gccaatggcc cgatgggtgc atggttggta catcacaacg
    10021 tattgcccca cgatggcaac gtgttgtgtg ttaaaggcca tcaggggcgc gcattggggc
    10081 gtgacggcat gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa aaagtcacca
    10141 tttcaggcac agcggtgatt ttgtttcatg ccgaatgggc aattgaaatc tgaaatcgtt
    10201 gcctgatgcg cttcgcctgt caggtctaca accgggctaa tgtatgccgg ataaggcgtt
    10261 tacgccgcat ccggcaacct gacataaatt attttcccgc ctccggatgc gtatcaaacg
    10321 ccgccatcac cagcgctaac tcatccacag taaagccatg tttcgcatca tccacgccca
    10381 gaccaaactg ttcctgcatc acggcatata acgacgccac atccggtaga tttcgctgct
    10441 ccaccgcgtg cccattttca taatgggtaa aatgaaaatt ggtcagtgtc agcttgccgc
    10501 cgtccggcaa atggcggcac atcagcaaat gatggcgaaa atgcgactgc ggccagtgcg
    10561 ccgaccagaa attgcccatc acataatcgc tttgttgctg ctcgcagaga tcgaagcggt
    10621 acatcggctg ccaatgctga tgatgattaa actgcaacac ccaatcatca ccctcctgca
    10681 acaaccgata ctctccgtgt ggcgtggtct gcacgagatc ggaaactaaa cgaatcggcg
    10741 cggttagcgt ctgcccaccg aaaccgacat cagcaatcca tttttcccct tccagttcca
    10801 ccaacagcaa acgatgggtg cgcggcggta atgctggcgg atttgataac actacgcgcc
    10861 ctaacaagct gcgaacgtta aaccccagct cgcgtaacac ccgctcaaac acgccattct
    10921 gctcaaaaca gtaaccgcca cgacgggcta tcaccagttt ctcttccagc gattgatcat
    10981 caagctgtat ttccctcggc agcaaaacgt caaggttttc aaacggaatg gtgcaattgt
    11041 gtttcaggtg caatgcacga agcgtatcaa tattgaccgc agcagctccc gaccagttaa
    11101 tacgggcaaa atagtgattc agaatgggcg tcatcaggcg tttcccttag tggttttctc
    11161 aacttataac ctggaataga cgccgcaccg gctattttgt gcgatcgcgg acatgcatca
    11221 cgtttgcaaa tcgccaatcc cgttaatttc gttacttatt gcgttacgga ctacgcttaa
    11281 aaaaaccttc ttcgcgaggc gataccatga gccgattcat ccccatagaa ttacaccatg
    11341 ccagccgtct gttaaatcat ggcccaactg tcttgatcac cagtttcgat gagcaatccc
    11401 agcggcgtaa cattatggct gcagcctggt caatgccggt ggagtttgaa cctccacgcg
    11461 tggcaattgt ggtagataaa tcgacatgga ccagggagtt gattgaacgt aacggtaagt
    11521 ttggcatcgt tatcccgggc gttgcagcaa ctaactggac gtgggcggtg ggaagtgtgt
    11581 cggtgcgtga agaagataaa tttaattgct atggcattcc ggtggtgaaa ggtccggtgc
    11641 ttggtttgcc acttgttgaa gagaaatgcc tggcgtggat ggagtgccgc ctgctacctg
    11701 cgacttctgc gcaacaaaaa tacgacacgc tgtttggcga ggtggtatca gcagcggcag
    11761 acgcacgggt atttgtcgaa ggccgctggc agtttgatga tgataagctc aatacgttgc
    11821 atcatttagg tgctggcacg tttgttacta gcggcaagcg tgttacggca ggttaagcat
    11881 tacatgctgt aaccgggttt ctttatcaaa gcctccattt ggggctcgat ttcggcatcc
    11941 cagatagctt gccaagattc tggttgaatc tgctgtagag caatgcttat cgagctatct
    12001 ttactgttca gatggcgaat aataacgtcg gtaatatctg cagcaagtgc tgctttttgt
    12061 tgttcgtcca gttcacgcgg aaagcattta atgtcgatgt gcggcatgat tacgctcctt
    12121 ttgaaagatg tcagtaaaat taacacacat tgtgaatgtc atgtgtccgg taaagcgttt
    12181 atacgacaat aatgcccagg gcaagattac gaaagcccgc tccccgtaag gactgacgcc
    12241 aggtagtttc tgcccatggc tgcttttcgc atcttacgtc ttaaccctgc cttgaatacc
    12301 ttatcattcg tcaaaatatt aatagcaatg tgccgtatcc ctgaaaataa ttctgctgca
    12361 tttcctcttc ttattttgca gtcgtcttca ttcattacca cgtccagacg cca
//
LOCUS       DAZPEP010000119        12409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZPEP010000119 DAZPEP010000000
VERSION     DAZPEP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.119"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            376..1200
                     /gene="gadX"
                     /locus_tag="ACHUT9_003159"
     CDS             376..1200
                     /gene="gadX"
                     /locus_tag="ACHUT9_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFR1349571.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1568..2296
                     /gene="gadW"
                     /locus_tag="ACHUT9_003160"
     CDS             1568..2296
                     /gene="gadW"
                     /locus_tag="ACHUT9_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFR1349572.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHUT9_003161"
     CDS             complement(2659..5772)
                     /gene="mdtF"
                     /locus_tag="ACHUT9_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFR1349573.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHUT9_003162"
     CDS             complement(5797..6906)
                     /gene="mdtE"
                     /locus_tag="ACHUT9_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFR1349574.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7014..7292
                     /locus_tag="ACHUT9_003163"
     CDS             7014..7292
                     /locus_tag="ACHUT9_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349575.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHUT9_003164"
     CDS             complement(7293..7820)
                     /gene="gadE"
                     /locus_tag="ACHUT9_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFR1349576.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHUT9_003165"
     CDS             complement(8619..9191)
                     /gene="hdeD"
                     /locus_tag="ACHUT9_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFR1349577.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHUT9_003166"
     CDS             9446..9778
                     /gene="hdeA"
                     /locus_tag="ACHUT9_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFR1349578.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHUT9_003167"
     CDS             9894..10220
                     /gene="hdeB"
                     /locus_tag="ACHUT9_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFR1349579.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10284..10931
                     /locus_tag="ACHUT9_003168"
     CDS             10284..10931
                     /locus_tag="ACHUT9_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFR1349580.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10973..11503)
                     /locus_tag="ACHUT9_003169"
     CDS             complement(10973..11503)
                     /locus_tag="ACHUT9_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFR1349581.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11659..12225)
                     /gene="slp"
                     /locus_tag="ACHUT9_003170"
     CDS             complement(11659..12225)
                     /gene="slp"
                     /locus_tag="ACHUT9_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFR1349582.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         3430 a         2754 c         2808 g         3417 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacatttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaatcaatt tgacttaaga gggcggcgtg ctacattaat taacagtaat atgtttatgt
      361 aatattaagt caactatgca atcattacat gggaattgtc taattgcgta cgcaagacat
      421 aaatatattc tcaccatggt taatggtgaa tatcgctatt ttaatggcgg tgacctggtt
      481 tttgcggatg caagccaaat tcgagtagat aagtgtgttg aaaattttgt attagtatca
      541 agggatacgc tttcattatt tctgccgatg ctcaaggagg aggcattaaa tcttcatgca
      601 cataaaaaaa tttcttcatt actcgttcat cactgtagca gagatattcc tgtttttcag
      661 gaagttgcgc aactatcgca gaataagaat cttcgctatg cagaaatgct acgtaaaaga
      721 gcattaatct ttgcgttgtt atctgttttt cttgaggatg agcactttat accgctgctt
      781 ttgaacgttt tgcaaccgaa catgcgaaca cgagtttgta cggttatcaa taataatatc
      841 gcccatgagt ggacactagc ccgaatcgcc agcgagctgt tgatgagtcc aagcctgtta
      901 aagaaaaaat tgcgcgaaga agagacatca tattcacagt tgcttactga gtgtagaatg
      961 caacgtgctt tgcaacttat tgttatacat ggcttttcaa ttaagcgagt cgcagtgtcc
     1021 tgtggatatc acagcgtgtc gtatttcatt tacgtctttc gaaattatta tgggatgacg
     1081 cccacagagt atcaggagcg atcggcgcag ggattgccga accgtgactc ggcggcaagt
     1141 attgttgcgc aaggaaattt ttacggcact gaccgttctg cggaaggaat aagattatag
     1201 aattttactc agacataaaa aaaacccggc ataggggacc gggaagagga tagtctgccg
     1261 tctccagact aataaaccgt tataacactc cctgttggca cgggaaactt tgtgctctca
     1321 gtaagttaaa tataactttt actggaaata agatcagcca tttttttata aacataagct
     1381 atacgctgtg cgaaaatata ttcgtgctgc atttacttat tatcaattaa ctgttatgca
     1441 aaactacttt gtggataaat tttggtccta ccaaatctgg cagttttttc gctaagaaac
     1501 agtctggcaa catttcatta gtatactgaa attgaaataa tcgcagtatg aaatataagg
     1561 gataatcatg actcatgtct gctcggtgat cctcattcgt cgttcattcg atatttatca
     1621 tgaacagcat aaaatatcgc tgcataacga gagtatcgtg ctgctggaga aaaatttggc
     1681 agacgatttt gcgttttgtt caccggatac gcgacgactg gatatcgatg agctgacagt
     1741 ttgccattac ttacaaaata ttcgtcagct accacgcaat ttagggttac atagcaaaga
     1801 ccgtctatta attaaccagt caccccccat gccgctggtg acggcgattt ttgatagctt
     1861 caatgaatcc ggggtaaatt caccgatact gagcaatatg ctctaccttt cctgtttatc
     1921 gatgttttct cataagaaag aactgatccc cttacttttc aatagcatca gcactgtttc
     1981 aggaaaagtt gaacgcctta ttagctttga tatcgccaaa cgttggtatc tgcgcgatat
     2041 cgcggaaaga atgtatacca gcgagagtct cataaaaaaa aagttgcagg atgaaaatac
     2101 ctgtttcagt aaaatattac tcgcttccag gatgtcgatg gccagacgat tactcgagtt
     2161 acgtcaaatt cctctgcata ctattgcaga aaaatgtggc tatagcagta cgtcgtactt
     2221 tataaacaca tttcgacaat attatggtgt aacgccacat cagtttgcgc aacattcgcc
     2281 aggtaccttt tcctgacata ttttgcattt gaatattggt caggatctca cacctgcttc
     2341 atgtgaaact cttccctgat gatttctgcc gggctaccgg ctagttctct ttcgcagttt
     2401 gccaggcggt ataggtattt tcaggaggaa aagactcggc atgtttggga ttattaagct
     2461 gacaattcat acgattacgg gaatgtgggt cagtattgtg ctgttcaaat tgatgacgaa
     2521 tggctggagt ggtttctact ttcagtgctg tgtactaagc ctggtattcc tgacggttag
     2581 ctggttgttg agtggtgaat ggttagcagg aaagagtaag gctgaacctt cacgttcaac
     2641 cttactctca tttacacgtt acgctttttt aaagcgggca aagagatgtt ccactacaac
     2701 gaaaaagacc ggaacgaagt aaattgccag cactgttgcg gcaaacatcc cgcccatcac
     2761 gccggtacct accgcgtttt gcgcgccaga accggcacca tgactgataa ccagcggcag
     2821 cacgccgaga ataaaggcga gagaggtcat caggattggg cgtaaacgca tccgcgccgc
     2881 ttcgatgatt gcctctatcg gcgttttccc ttctttctgc atcatctcaa cggcaaattc
     2941 gacaatcagg atggcgttct tggcggaaag cccgatggtg gtcagcaaac caacctggaa
     3001 gtagacgtca ttacttaagc cgcgcagatc ggtggccagt aatgcgccaa cgacgcctaa
     3061 cggaacaacc aacatcaccg agaacggaat tgaccagctc tcatagagtg cggcgagggc
     3121 gaggaacacc acgaccagtg aaatggcata cagtgcagga gcctggtttg aggataacgc
     3181 ttcctgatac gacagtccgg tccatgagta gccgacgcct gccggaagtt tagcgaccag
     3241 gtctgccata aatttcatag cgtcaccggt acttttcccg gccgccgctt cacctaaaat
     3301 ctccattgac gggatgccgt tgtagcgttc caatcgcggt gaaccatagg tccattcggt
     3361 agacgagtag gcagaaagcg gtgccatcgt gccagaggcg ttgcgtacat accattggtt
     3421 gatgttatcc ggcaacatac ggaacggcgt gcctgcctgg acatacactt ttttcacccg
     3481 cccctggttg aggaagtcgt tcacgtagct gctgccgaag gcggtggaaa ttgtctggtt
     3541 gatatcagac agcgccacac ccattgcctc agctttcgcg gcgttgacgt tcactttgaa
     3601 catcggcgta tcttccaggc cgttcgggcg taccccggtg acctggtcag gcgattgcgc
     3661 tgccagtgat aacagctcgt ttcgtgcctg ggttagtttt tcgtgcccca ggttaccgtt
     3721 gtccagcagt tccatatcaa aacctgacgc ggtacccagt tcagccaccg cgggtaagtt
     3781 gaacgggaag acgacggctt tattgatact gcttaacgca atcattgccc gctgaatgat
     3841 cgcggtaacc gagttttcct caccgacacg ttcagaccac ggcttgagac tgataaacgc
     3901 caggccgttg ttttgcccct ggccgctgaa gccaaagccg ccaacggtaa acaccgactg
     3961 aacattatct ttctcttttg taagataata atccgtcacc tgttgcagca ctttcgtggt
     4021 gttaaccatg gtggcaccgg aaggtaactg cgcggtggtc ataaataccc cctgatcctc
     4081 ttctggtaag aaagaggtcg gtgtacgcag gaacagcacc gccatcccgg cgcaaatcag
     4141 caggtagacc accatgtagc gaccggtaca acgcaacagc gagcgggtgc tatcggtata
     4201 gtgctgagtt gatttttcaa acagcgtgtt gaagcgtgcg aacagggcgt taggtttgtg
     4261 accgccttcc ggcgcggctt tcagaatggt ggcgcacagg gcaggggtca ggctcattgc
     4321 cacaaatact gaaagcagca tggaggagat cagcgtgatg gagaactggc ggtagatctc
     4381 cccggttgcg ccgctcataa aggccatcgg cataaacact gcggaaagaa caacagcaat
     4441 accgaccagc gcacgttgga tctgccccat cgatttatgc gtcgcttcct tcggtggtag
     4501 cttatcttcc gcaatgacac gctcgacgtt ctccaccacc acgatggcgt catccaccag
     4561 taaccctatc gccagcacca tcccgaacat agttaacgtg ttgatggtga aaccgaccgc
     4621 cgacaagatc gcaaacgtcc cgagaataac caccggtacg gcaatcgtcg ggatgattgt
     4681 ggcacggaaa ttctgcaaaa acagatacat gaccaggaag acgaggatga tagcctcaac
     4741 cagtgtttta aaaacttcct gaatagaaat ttcgataaac ggcgtggtgt cgtaaggata
     4801 aaccgtcttc agacttgcgg ggaaataggc tgataagcgg ttcagctcct ctttgactgc
     4861 ccgcgaggta tccagggcgt ttgctccggt agccagtttg atggcgatcc cggcagcagg
     4921 tttgccgtta tagcgcgcca cggtggaata atcttccgct ccaagttcga cgcgggcgac
     4981 atcacgcagc agcacttgcg aaccatcttg ctgaactttc aacaggattt tgccaaattc
     5041 ttccggcgtt tgcagacgcg tctgcacaat gatcgaggcg tttagttgct ggtctgccgc
     5101 ctgtggcatg ccacccagtt gaccaccgga aatctggttg ttttgcacct taatctggga
     5161 aataacatcg gaaggtacca gattgtattt attgagtttt tgcgggtcca gccagatacg
     5221 catggcgtac tcggaaccaa agagctgtac gctaccaacg cccgcggtac ggcttagcgg
     5281 gtctttgata ttagacgcta catagtccgc gatatcgtac tggttgaggc tgccgttatc
     5341 agaaataaac gccgctacca tcagaatatt actgctcgac ttatcaacgc taatcccctg
     5401 ctgctgcacc gcttcaggta atgaaggcat agcgagttgc agtttatttt gcacctgaac
     5461 ctgtgcgata tcaggagatg tcccagtctc gaaggtcaga gtgatagagg cattacccgc
     5521 cgcatcactg gttgaagaca tgtacatcag gccatcaagc ccattcatat tttgctcaat
     5581 cacctgagtg accgagtctt ctaccgtttg cgcatcggca cctggatagg tagcgctgac
     5641 ggtaatggtc ggtggcgcaa tctgcggata ctgcgcaacc ggtaagttca tgatcgccag
     5701 accacctgca agcatcataa taatggcaag tacccaggca aaaaccgggc gatcaataaa
     5761 atagttagcc atgaaagtcc ccttaagcct gcaacgttat tgtttcgatt cggtgctggc
     5821 gttttcctgg ctggaggaaa ttgctcgtgc tttgataccc ggacgaatgc gttgcaaacc
     5881 ggaaacgatc acccgatcgc cagcctgcaa gccagaggtg acaacccact ggtcgccgat
     5941 ggctttgctg gcttcaattt cgcgtagctg cacaacatcg tctttatcca gaatgagcgc
     6001 cgttgcttta ccctgggcgt tgtgagtgac gccttcctgc ggcaccagta atacattctg
     6061 gcggctacct tcatccacta atgccgtgac gtacatgcca ggcagcaagt caccatttgg
     6121 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatccaccg tcgggtcgga
     6181 gaatttcagt gtgccggtct ggctgtagcg tttaccattt tccagattga gctgtactgg
     6241 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgcaagaa
     6301 atcctgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
     6361 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
     6421 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
     6481 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
     6541 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
     6601 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
     6661 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
     6721 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
     6781 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccggggg agagtgtgac
     6841 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
     6901 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
     6961 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
     7021 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     7081 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     7141 gcattcgggc aacgaagggt tctactggtg gatacacata cctggggaat aaagtcgttt
     7201 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     7261 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     7321 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     7381 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     7441 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     7501 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     7561 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     7621 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     7681 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     7741 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     7801 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     7861 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     7921 tttacccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     7981 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     8041 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     8101 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     8161 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     8221 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     8281 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     8341 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     8401 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     8461 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     8521 atagtgcgca attgatctac aacactccgt agcggagaga gtattaatcg gatcatagtc
     8581 atatcaagtg actatgatcc gggtgacaac cgggataatt attgctgctt aacgaacaaa
     8641 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     8701 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     8761 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     8821 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     8881 atgcccagct ccggtgcgcg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     8941 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     9001 agcgcaatgc ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     9061 acgaacggga aactgataca cagcaacccg acgataaaca gtagcacggc aataaactgg
     9121 attgctctgc gatgtttttt aagcatctcc agatcaaact tcaaaattgt tgccttatct
     9181 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     9241 ccaaaaacgc gtctaagaat gtagtcgatt taataaaaat ttcctaatta cagtatctga
     9301 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tatatcaaca
     9361 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     9421 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     9481 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     9541 accggtcaac tcctggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     9601 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     9661 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     9721 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     9781 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     9841 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     9901 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     9961 acgcacaatc tgcgctggca gccaatgaat ccgctaaaga tatgacctgc caggaattta
    10021 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
    10081 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
    10141 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
    10201 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
    10261 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
    10321 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
    10381 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
    10441 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
    10501 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
    10561 taggtctgac gacggcagcg gatatctggg tgaccgccgc cataggtatg gttattggca
    10621 gcggtatgta cgaactgggt atttatggtt cagtgatgac cttgttggtg ctggaagtct
    10681 tccatcaatt aaccttccgc ctgatgaaca aaaattacca tttacagctg acattagtga
    10741 acggtaatat ggtatcaatg ctcgactggt ttaagcagca aaaaataaaa actgatttag
    10801 tttcgttgca ggaaaatgaa gatcatgaag tggtcgccat tgatatccag cttcatgcca
    10861 caacgtcgat agaagacctg ttgcgattat taaaaggtat ggcaggggtg aaaggcgttt
    10921 caattagcta atctgtgtgg cagcgtagcc agactcaccg taagcctgaa attcacacca
    10981 gataatcaat atgctgatgg cgtaccagct cattgatccg cttaagattg aggatcatca
    11041 tgatattgtg tttatggcag tagaacgttt ttagcgatat tttcagtttc tccagaatat
    11101 cttcagttgt catttgattc gcaatgcagc tcagaacctg atgttgcttg cgcgaaagtg
    11161 acacattcgc ttcaggcaca gagcagtaac tgccattgat gagtgcgaca aaatcaatga
    11221 tagtgatagt ccgcggaaca aaggtcaccg gtactttccc cagacagcgt ttaatattaa
    11281 agggggagaa aacaatgaca tggacaggtt ttaatcgttc aatttgagtg agaaaattag
    11341 aatgaaatac attattcatt aatgtatcaa taatgacgaa ggcattctga tgcaacatta
    11401 cgtcgatctc ttcttcgttc tgtatatgaa cgacattacc tttactcaga atgtttttca
    11461 tcgcggtgaa gaacatcgta tccctggtaa ttataagaaa cataaccgac tcctggtgcg
    11521 gacattaact ttataatcaa tttgccaaag gtaattaaca ggacagggct tataataata
    11581 atagttagct tactgtatat taattttatg taatacatga tgcagatgct gagaaagcat
    11641 ctgcatcttt cggtggtgtt atttgaccag ctcaggtgtt acctgactca ccgcattggt
    11701 gtagtaaggc gcaccccagc ccggttccgg ccagaatgca ccatagccgt aatcccacag
    11761 gttataggtg gtattaacca cttctctcaa atgccacacc tggatgccct gcatattcac
    11821 ttccaggaag ttatacggga ctttattgat aaagccaggt tgttcaccct gaatggtacc
    11881 gaggatggta acaaagtgat tacgatagtt cactgggtca aggaagccgc tttgtctggc
    11941 gagcagtcgg ccctgatagt tggcttcaat atcaggtttc gcatagctat ccaacggtaa
    12001 tacagcgatt tctaacaacg tatccgtttt gccattgata acgttgataa ccttcccacc
    12061 aaagcgcgct tgttgaccaa catataaccc cggctggtta tgaacagcaa caaaactttt
    12121 ttgaatatca ggttggttat tgcctttgat attttgcgga attgaactac atgcggcaag
    12181 caaaaatgaa aggctgagga tgagtgcacc ttttgtcatg ttcatgttac tatccttatc
    12241 aacaaatatt aaaaccataa tagatatttc tttatagttt tcatctgatt ctgaggcgat
    12301 atgcctgata gtaacgtaaa cctgtctgat gtttatatcc cttttgttta gttcaattcc
    12361 attgattttg gggcgtggca tgagtatcca tcgccccata atataatcc
//
LOCUS       DAZPEP010000120        12191 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZPEP010000120 DAZPEP010000000
VERSION     DAZPEP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12191
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.120"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..71
                     /locus_tag="ACHUT9_003171"
     CDS             <1..71
                     /locus_tag="ACHUT9_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR1349583.1"
                     /translation="YEALRTVDRLLRGQSFIKQADL"
     gene            82..369
                     /locus_tag="ACHUT9_003172"
     CDS             82..369
                     /locus_tag="ACHUT9_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349584.1"
                     /translation="MTKDNAQGVFIRFIDFRGELLLRASAIDGVTPAGKNGADEATYV
                     YLNGTRLLVELPYQTVREIISEDEKARQVNGDEPYIEIICMDSEAEIQKAD"
     gene            381..623
                     /locus_tag="ACHUT9_003173"
     CDS             381..623
                     /locus_tag="ACHUT9_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFR1349585.1"
                     /translation="MGKEYKTLINKALERFYFRLSASGAHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            620..733
                     /locus_tag="ACHUT9_003174"
     CDS             620..733
                     /locus_tag="ACHUT9_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349586.1"
                     /translation="MSIFISWLVLIISVVCAIGIMRIINSVKKIERFFTEE"
     gene            820..1023
                     /locus_tag="ACHUT9_003175"
     CDS             820..1023
                     /locus_tag="ACHUT9_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349587.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVVQVNLIRQVQESRR"
     gene            1020..1265
                     /locus_tag="ACHUT9_003176"
     CDS             1020..1265
                     /locus_tag="ACHUT9_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFR1349588.1"
                     /translation="MTILDYIAANPGCSSGEIAAALNTPTTTINAELRRLWRSGLVTR
                     KERKTGGRFSYQVNLMPFGCSNPLTQMFNQLLREIRA"
     gene            1291..1410
                     /locus_tag="ACHUT9_003177"
                     /pseudo
     CDS             1291..1410
                     /locus_tag="ACHUT9_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1407..1772
                     /locus_tag="ACHUT9_003178"
     CDS             1407..1772
                     /locus_tag="ACHUT9_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349589.1"
                     /translation="MILANDFLEYLLNTERDLAARVRDRYDMYLKSLPVPQLADGKIV
                     IDGRYMIDSHEGNYRLYRIEGGTPSVIGIYQRPSSAIVDVIADSIRITHRHADTEDTV
                     LEIQRLATVCRDTLNGMTK"
     gene            1779..4601
                     /locus_tag="ACHUT9_003179"
     CDS             1779..4601
                     /locus_tag="ACHUT9_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFR1349590.1"
                     /translation="MTAEYIRDWQQPRHAVGREGTGIPAPESALSSWLDAHRAENERR
                     QEMADAAFSATPLGNLINKSLDAQEKQDKTITLAGDARKQARGAVDEAMASLRLLPSY
                     LRDPLIRHLSFLRKKQEADRRKGKKSWQAERYARGTLRKIFERLDRTDGHWLTPGYRS
                     LAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTMDVTLKA
                     YQMLARMALHLHAMPPHYDALTTDKDRRHEPDTELLPGAILRLTCAEWWKRKLWLLRC
                     EWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFTIDLETV
                     YYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGHPNPKWN
                     GATMRDSSDYLVNTFFATVRKKLNRDGLRWYGIRTAEPHHDGTVHWHMMVFAHPEEID
                     TIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDSRAVDGI
                     DPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRRLASQMA
                     RNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLADELNDY
                     GEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGPAAPWTR
                     GNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWMRSLKRHERRALMRSLRDKQA
                     KNSSDEMQSWTQSRKQQRPLPDNHELLAKEWRESAESLGLHIGEQQMQHLLRGGSLYV
                     DDSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLTQLGASD
                     PEAAARLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKADLLRGG
                     LTSGNKKQTETGLTNPVNEQKTRSDI"
     gene            4678..5637
                     /locus_tag="ACHUT9_003180"
     CDS             4678..5637
                     /locus_tag="ACHUT9_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFR1349591.1"
                     /translation="MKICIDDGSTNIKLAWTENGERRNAISPNSFKSEWSAPFGGMQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            5642..5953
                     /locus_tag="ACHUT9_003181"
     CDS             5642..5953
                     /locus_tag="ACHUT9_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFR1349592.1"
                     /translation="MTTPTRRISFYLKPAAVKNESEACAWLGSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPVPQPVPTAQITSQIAGNIRALFP
                     E"
     gene            6014..6538
                     /locus_tag="ACHUT9_003182"
     CDS             6014..6538
                     /locus_tag="ACHUT9_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349593.1"
                     /translation="MKKYEYMLMKEALRAGLAPCPVSVEAVVGNTIFRTLRNRVSVKM
                     VHQPPKSTSMIYLPLPEAIKLMRSLKFAIWMQTQINKAVQNTFVKRLIPELAARCAKC
                     TRPALQFDLSLIQQATEAELYRLSPIRDAHTEASEMFRLMFQFNFAVRRTRVQVNRMM
                     QEQEELTQYKGGSQ"
     gene            6535..6774
                     /locus_tag="ACHUT9_003183"
     CDS             6535..6774
                     /locus_tag="ACHUT9_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349594.1"
                     /translation="MSNSARLQLGFSPLSKTIVLAKMRDLGDGTKRRVGNDRGRDVTN
                     EAAQLVWHLVMAEGGEINWELDDGSRMILRAEKSK"
     gene            complement(7466..8512)
                     /locus_tag="ACHUT9_003184"
     CDS             complement(7466..8512)
                     /locus_tag="ACHUT9_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1349595.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            complement(8512..10263)
                     /locus_tag="ACHUT9_003185"
     CDS             complement(8512..10263)
                     /locus_tag="ACHUT9_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFR1349596.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDATATGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAEMHFLGTSAATAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTCFS
                     TPSTETHEAYAYWNGDRWNEKKAAHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFRNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTYRYNVEHIGIDATGGHGDAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            10418..11254
                     /locus_tag="ACHUT9_003186"
     CDS             10418..11254
                     /locus_tag="ACHUT9_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFR1349597.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVESVATS
                     QADIIDRFNALETRHQQDSQKITLLTTELTALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            11278..>12191
                     /locus_tag="ACHUT9_003187"
     CDS             11278..>12191
                     /locus_tag="ACHUT9_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFR1349598.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPAVQQR
                     FENASKDSTELTKKINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWSAHSDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTNASERVMNDVTLTSRNMDNTVAHAGKYANPDALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQ"
BASE COUNT         3100 a         3301 c         3198 g         2592 t
ORIGIN      
        1 tatacgaagc acttcgcacg gttgatcgtc tgcttcgcgg gcaaagtttt attaaacagg
       61 ctgatttata acaggtgctt tatgaccaaa gacaatgcac aaggtgtatt tatccgtttt
      121 attgattttc gcggtgaact gttattacgt gcatccgcta ttgacggagt gactccggcg
      181 ggtaaaaacg gagccgacga agccacttac gtttatctga acggcacgcg actgcttgtg
      241 gaacttccgt accagaccgt acgagaaatc atcagcgaag atgaaaaggc acgccaggtt
      301 aatggcgatg aaccctatat cgaaattatt tgtatggatt cagaagctga aatacagaaa
      361 gcagattaaa gggcgttgtg atgggcaaag aatataaaac tctcattaac aaagcacttg
      421 agcgttttta ttttcgctta agtgcatcag gcgctcatgc tgaacgtgcg gcccgtgact
      481 cattgaccag agcaatccga agtctgtatg acgtggcttt ttacgctgat gatctggatg
      541 cacttaacga actttccgag ctgatctgtg ccgcagaatg cggggaacat attgaaccgt
      601 ataagctggg aaatatcgca tgagtatatt tatttcatgg cttgttctga ttatttcggt
      661 ggtctgcgcc attgggatta tgcgaattat taattcagta aaaaagattg aacgcttttt
      721 cactgaagaa taaccgcgca aataagaccc caggttaaat aagaaaatgt gaaaacaatc
      781 cgcattcgcg gaggtattcg cacacgccaa ggaggcgtaa tggcaattaa gcattttcct
      841 gtcgttcgtt tcacctccag aggacgtgaa tacgaagttg acgaacgcct gattaccaca
      901 atcgacaaac accgttcaga aaaggatgca catcatatct atctcactga cggcacttac
      961 ttctgcgcca ccaacgtggt gcaggtgaat cttatcagac aggtacagga gtcacgcaga
     1021 tgaccattct ggactacatc gctgccaatc cgggttgtag cagtggagaa atcgccgcag
     1081 cactgaatac accaaccaca accattaatg cggagctacg ccgtctctgg cgcagcggtt
     1141 tagtcacaag aaaagagcgc aaaacaggcg gtcgcttttc ttatcaggta aacctgatgc
     1201 cgtttgggtg tagcaaccca ctaacccaga tgttcaacca gctactgagg gaaatcagag
     1261 catgagcacc tccaactgcc ggaaaccacg tcgggcttca gcagctcatc cggcagcaaa
     1321 acaaactcca ttaattcctg ttccgggcct ttcctgcacc ttgcggcggg aggccttcgc
     1381 acatctgtaa caagaggatt gccgcaatga ttctcgccaa cgactttctt gaatacctgc
     1441 tcaacacaga gcgtgatctt gccgctcgcg tgcgtgatcg ttatgacatg tacctgaaat
     1501 ccctgcctgt accgcagctc gctgacggaa agattgttat tgatggtcgc tacatgattg
     1561 acagccacga gggaaattac aggctttacc gcattgaagg tggcaccccg tccgttattg
     1621 gcatttacca gcgcccatcc tctgcaatcg tcgatgtgat tgccgacagc atccgcatca
     1681 cacatcgcca tgccgacaca gaagacaccg tgctggaaat tcagcggctg gctacagtct
     1741 gccgcgacac cctgaatggc atgacgaagt aaatcactat gacggcagag tacatcaggg
     1801 actggcaaca accgcgccac gcagtggggc gtgaaggaac ggggatcccc gctcctgaat
     1861 ccgcgctttc ctcctggctg gatgcccacc gggcagagaa cgagcgccgc caggaaatgg
     1921 ctgatgcggc gttctccgcc acgccactgg gcaacctgat taataaaagc ctggacgcac
     1981 aggaaaaaca ggacaaaacc atcacactgg caggagacgc cagaaaacag gcacgcggcg
     2041 cggtggatga agccatggcc tcgctgcgcc tgctgccgtc ctatctgcgc gatccgctta
     2101 ttcgccacct ctccttcctg cgcaaaaaac aggaagccga tcgccggaaa ggcaaaaaga
     2161 gctggcaggc ggaacgctat gcacgcggaa ccctgcgcaa aatattcgaa cgtctggatc
     2221 gcactgacgg acactggctg acaccgggtt atcgctccct tgccggacgt gaacgcctgg
     2281 acgatttgct ttacctgccg caactcaaca aacaccagat acagacgctg gccaccatga
     2341 cggcggcgat gttcagcagc accttcgaaa aactctgcga tggttttggc gcgactgatg
     2401 gcgagctgac catggatgta acgctgaagg cgtatcagat gctggcccgc atggcgttac
     2461 acttacacgc catgcctccg cattatgacg cactgacaac agacaaagac cggaggcacg
     2521 aaccggacac agaactgctg ccgggcgcaa tccttcgcct gacctgtgcg gaatggtgga
     2581 aacgcaaact gtggctgtta cgttgcgagt ggagagaaga acaacttcgc gccgcctgtc
     2641 tggtttccag aaaaacatcg ccctatctga gccaggacgc gttaagcgag tttcgcgcac
     2701 agcgcgagaa aacacgcgat ttcctgaaaa gtttcatgct ggaaaatgaa gacgggttca
     2761 cgattgatct cgagacggtg tattacgcgg gagtaagtaa cccggttcac cgtaaggcag
     2821 aaatgatggc caccatgaag gggctggaac ttctggccga agcccgtggc gacaaagcgg
     2881 tgtttctgac tgtcacctgc ccgtcaaaat accacgccac aacggagaac ggtcatccga
     2941 atcccaaatg gaacggggcc acaatgcgcg actccagcga ttacctggtt aacacgtttt
     3001 ttgcgacggt ccgcaaaaaa ctgaaccgcg acggcctgcg ctggtatggc atccgcacgg
     3061 cggagcctca ccatgacggc accgtgcact ggcatatgat ggtctttgct catccggaag
     3121 aaatcgacac cattgtgtcc cacacccgcg atattgccat tcaggaagat cgtcacgagc
     3181 tgggcgatga cataactccg cgctttaagg cggagtatgt agacggctca aaaggcacgc
     3241 caaccagcta catcgccacc tacatcggga aaaacctgga cagccgcgcc gtggatggca
     3301 tcgacccgaa aacgggcaag ccacgcgttg accacgaaac cggaaaatca atggccgaga
     3361 gcgtggaacg cgccatcggc tgggcgcgcc ttcaccgggt ccgccagttc cagttctttg
     3421 gcatcccctc ccgccaggta tggcgtgaac tgcgccgcct tgccagccag atggcacgca
     3481 acccggaagg cccgcaacgg ctgaaggatg acgcaatgga tgcggttctt gccgccgctg
     3541 atgccggatg ttttgccacc tacatagaga aacagggcgg cgtacttgtt ccacgcaaag
     3601 actacctgat tcgcaccgcc tacgacctcg ccgatgagct gaacgattac ggcgaacaga
     3661 gcgtacagat ttacgggatc tggtcaccac tcatcgggga atcctcccgt gtgtgcacgc
     3721 atccggataa ctggaagctg gtaagacgca aaccggaagc ggaagacagc gcccgcgaaa
     3781 atggttttga ccttcagggc ggccctgccg ccccttggac tcgtggcaat aactgtcccc
     3841 gtgtacagga aacggacaac aacgggacag aacagccgga agaacggcca gcaccgtggc
     3901 cgcagctccc tgacggcgtt gatgtggatg aatggatgcg ctcactgaaa cggcacgaac
     3961 gccgggcgct gatgcgttcg cttcgtgaca aacaggcaaa aaacagcagt gatgaaatgc
     4021 agagctggac acagagccgc aaacagcagc ggcctttgcc tgataaccac gaattactcg
     4081 ctaaagaatg gcgggagtct gctgaatctc tcggcctgca tatcggtgaa caacagatgc
     4141 agcacctgtt acggggcggc agtctgtacg ttgacgacag catcattgca ccgcagggat
     4201 ttgaaattgt acgcaaaccg gatacccgcc cggacagccg aatcacgcag ctctggcagc
     4261 gcctgagccg taatcatggc gtaagcagca cggagatccg ccataacccg gtcgccagct
     4321 atctgacaca gctgggggca tcagaccctg aagccgccgc acgcctggca tccacacttc
     4381 agcaggacca gaacaccatg aaaacacccg ttaccgtgct ttctgacatg ctgcgcgcca
     4441 tccgcgacgc agagcacgca cagagaatca gtgaaaccac tgaacgcgcc cgccgcaaag
     4501 cagacctgct gcggggtggc ctgaccagtg gaaacaaaaa acagacagaa acgggactca
     4561 cgaatcccgt aaatgagcaa aaaacgcgca gcgatatatg aagcgcgcac aaaacaggca
     4621 aaaacgggat ttcagaatcc cgtaaacgat taattaatca acataaggaa aagcgacatg
     4681 aaaatttgta tcgacgacgg ctccaccaac atcaagctgg catggactga gaacggcgaa
     4741 cgccgcaacg ccatcagccc gaacagcttc aagtcggaat ggtctgcgcc gttcggtggc
     4801 atgcagcccg cgaactacat gcttgatggc gtgcgctatg gttttgatcc ggtcagcgat
     4861 cgctttgtcc agacaaccga cacgcaatac cagtacagcg atgtaaatgt cattgccatt
     4921 catcacgcgc tggtcaaatc aggcatcacg ccacaggagg tggatgtggt tgtcaccctg
     4981 ccactgagcg agtatttcga cacaaacgca cagccggaca tggccaacat caaccgcaaa
     5041 aaagcgaacg ttatgcgccc ggtggagtac cagaacggcg aagcattcac tatccgtaac
     5101 gtgcgggtta tgcctgaatc cattccggct ggctttaagg cactggctga catgagtccg
     5161 tttgaatccc tgctgattgt ggatttgggc ggaaccacgc tggatgtggc aaaggttcag
     5221 gggcaactgg caggtatcag ccaggtgttt tgcgatccac acgtaggcgt ttctctgatg
     5281 gccgatgccg tactgtcggt gatggccact aacggtatgc gcaccagtca ccacatcgcc
     5341 aataccatta tcgaacatcg ccatgatgaa gcctggctgc gccagcacat ccacaatgac
     5401 gcgcattacg ccagcctgat ggcggttatt cgtgaaaagg aagaaacact gaaacaacgc
     5461 gtgatccgcg cgctggcggg tttttcgggt tacgggcggg tgatggttgt cggtggaggg
     5521 gcggagattg tggcacccgc tatccgcgaa gcctgcggag ttaatgcgac tttcatcgcg
     5581 gacggggtgc cacagtttgc tctggttaat gggctgtacg caatggacaa ggagtaaacc
     5641 aatgacgaca ccaaccagac ggataagttt ctatctgaag cctgcggccg tcaagaacga
     5701 aagcgaagca tgcgcctggc tgggcagcct tacaccagaa gcccgcaaaa gcggccaacg
     5761 cgtggctttt ctggccgggc tggcacttct gaaaatgaat ccagcagagg cttaccgact
     5821 ggctgcatgg gctgacgatg aggcgttatc ggtgacacaa accaggacag aacgccccgt
     5881 gccacagcca gtaccaaccg cacagataac cagtcagata gcagggaata tccgggcatt
     5941 atttcctgaa tgacgaattt gacggctttt aattttatcg gggtgcactg tcgccccgca
     6001 cgaggaaaac acgatgaaaa aatacgaata catgctaatg aaagaagcac tgcgggctgg
     6061 tctggctccc tgtcctgtat ccgtagaagc tgttgttggg aacacgattt tccgaacgct
     6121 gcgaaatcgc gtatccgtga agatggttca ccaaccaccc aaatctacca gcatgatcta
     6181 tctgccgctg cccgaggcta tcaaacttat gcgttcgctg aaatttgcga tttggatgca
     6241 gacacaaatc aacaaagcgg tgcaaaacac cttcgttaag agactaatac cggagcttgc
     6301 tgccagatgt gcaaaatgta ccagacccgc acttcaattt gatctgtcat tgattcaaca
     6361 agctacggag gcagaactgt accgcctgtc acctatccgt gatgctcaca cggaagcatc
     6421 agaaatgttc cggctcatgt ttcaatttaa ttttgccgtt cgacgcacac gcgttcaggt
     6481 caatcgcatg atgcaggagc aggaagagtt aacacagtac aaagggggca gccagtgagt
     6541 aattcagcac gactacagct tggtttttca ccgctatcaa aaaccatcgt attggcaaaa
     6601 atgcgtgact taggagacgg aacaaaacgc cgcgtaggaa atgatcgcgg tcgcgatgta
     6661 accaacgagg ccgctcaact cgtttggcat ctagtcatgg cagaaggcgg tgaaattaat
     6721 tgggagctgg atgatggttc tcgtatgatt ttgagggccg agaagagtaa atagctcaag
     6781 ggggattaat gctaaatgat gatgaagaag agctgcttat gcaggaatgg tcgctcggtg
     6841 attatgataa tggtgaaaat gggtgcccgc attgtggccg ttatcgttta tgtatctgcc
     6901 agaatggaaa acacagatgt gaaaagtgca attggtctcc ggaacttaat gactacgtgc
     6961 cgatcgagtg gtagaggtaa gtcacactag gtgtttttta gttgatttta taaatgcaca
     7021 atagtgcaca aatttgcaca atttttttga acgacttttt gcccttccgg cccgcgtggc
     7081 ggctggatcc gtcaaggatc cgtgcatgca caaaaaaacg cgttttttct gcgcgcaggt
     7141 gacgggggaa cagcccgcgt ttcagggggt aaatagcatt ccctgaacga tgtcgcagag
     7201 atacaacaga atggctgtat ttctcacgct gagcgtgaaa aagacgtgag ggcttttgat
     7261 ttgatggggt gaaaggtaag gccgtcaaaa tcgcactgag acggcgagaa tatgcagtca
     7321 acgaggtggg attgcgtaag agtctgactg tcgatgatgg caatcagcag gaaagcgtcg
     7381 tgaaattatc tgactgatac aggagctgga gagtcggggc ataaattttt tatgccccgg
     7441 cgaagcagca gacaagcgaa gcgcgtcagg atgtgggctg ggtgtctaac agtgcgtaag
     7501 ggttaaagcg gatcacctct tcgccaagcc agtcattgat gtgcttcatg gcctccatga
     7561 cgggcatcag ctcgttaatt gcgtaaaccc gcgcggcctt ctccacatca ccaaacgcac
     7621 ttttttcgcc cggcatcgcc cccatcagtt gcggcggaac gcggtgcgca gccagcacat
     7681 catcacggga tgccgcctta acattcatga actcatcctt tgcggtgatc tgctggaacg
     7741 gcaaaatttg caccccctct ttgcccccgt tgggcgcatg aatgagcacg tttttaaacg
     7801 caccaccacc acgtgccccc tgtagcgttt ctttcaggga gtccatgctt tcgcggttta
     7861 cctgcgctgc accgatgtag atgatgcacc cggcgtggga tccgttgtcg tagtacagtt
     7921 ttctgaacat gtccgccgaa tgagaaaggc tggccgagag taatgcgcca agatattccg
     7981 gcatgccgta gatttcctgg ttaatatccg gattcatcag gtggcacact ttgccaggac
     8041 gaaactgaaa cgcatccttg ccatcctgca cataccacca tgattcaaga tcgcttccgc
     8101 gtcgcatgta tttcgccagg gcgtgccgta atttaagcgg ttcgccgagc atattgctcc
     8161 gaagctcaag gaatgcgtta ccgaacacaa accagtccag cgccagcgcc gagaaatcct
     8221 gccgggaaag cagcgggtgc ggaatatagc agccgagcaa tacattgcgc ttaaagtaaa
     8281 gcgcagactg atgccaggac gtttgccggg cagctcttgc cagaccgtac cagtccaccg
     8341 gggtttcata ccaccgcccg ttatcagcac agtacatatt gtccagcagg tcatgcccgg
     8401 tcaggcggta aggaccatca aatgtgaatg cactgagcga cgattctttc ctgagcgcat
     8461 cagcgagatc aatgcgtgaa ctcatgcgca cttttttatt ttttctgctc atcagaactc
     8521 cataaccgtg aaacgctcgt tttctccttc gccgccaatc ggttcgttaa tgacagcaag
     8581 catggttgcc cacgcaaggt cgccgtggct gatccccctc gcgcggtccg tttcgtaagt
     8641 gataaaaccg cccggtgttt tcaccttacg cacggcgtta aaggccgcaa ccagctcgcg
     8701 ttcggcgcga tcgtattccc accgcccggc acgcattatt tgcagcattt tcagtaccag
     8761 cgaccgtttt gatgacagcg tgaaggtgta agggatggcg gcgggaaaaa accgtttcac
     8821 tatctgatag acggcatccc cgtgcccgcc cgtcgcatca atgccgatgt gttccacgtt
     8881 gtagcgatac gtgaactctt caataactct ggcctgctct tcaaattcca gcccctgaac
     8941 gcgtcgcgtc tccaccgttc tgaaacgacc accaggaaca gccggaggaa ccaccacgga
     9001 cacagcgccg ctgtcgccgt taccactgct gccgtttgcg tcatacccaa tccataccgg
     9061 acgattcccc atcgggcggg gggcaaaggg tttccagtct ttccagtcgt cgtatccgtc
     9121 aacaccgcag ccaatcagga tattcaggtt aaatgccgat tccccttcgc ggacaaactc
     9181 acacatatag agattgcgga actcgtcttc ggtgttttca tcacgaattt catcaatatc
     9241 ggtgtgtttc cagccgtgat taaccacatc ttccagcgtg acaatttgcc gccacgtccg
     9301 gtcagggcag ataagcccgt tatgcagcgt tttccagtcc acagaaaaac gctggcgttt
     9361 atgcgcggcc tttttctcgt tccagcggtc gccgttccag taggcgtatg cctcgtgcgt
     9421 ttcggtggat ggcgtggaga agcaggtgcg ccgcagtccg ctgagggttg ccatagcgcc
     9481 agccaccttg cgcagttcag caaagcgact gacccagaaa aattcatcaa aataaaaatt
     9541 gcccgtgtaa gattgcgccg tcgcagcaga agtgccgaga aaatgcatct ctgcgccgtt
     9601 ggagaggatg attttatcgc cccctttcag ctccacatcg acttcagccg cggccttctg
     9661 aataatgctt ttaaactgga acgcctgacg acgcgacgca gacaaaaaaa tctggttacg
     9721 ctggtaaggt tgtgccacat cgtcacgcag cgccatcagc agtgcttcct gtgcaaaata
     9781 ccaggtcgcc ccaatctgtc gggatttcag gatcatcctg ttacgtatcc cggcttccct
     9841 gcaaagcgtc agggagtcaa accagccccg ctgatgccac tccagcctgc tgatgatttt
     9901 ttcccgcagt gcggcaatct gttccggcgt gaaatgattt ttgagttttt tcgcccggcc
     9961 tttctttcct gtggccgtcg catccggctg gccatcatgc agttttttaa gctgccgggt
    10021 cagcaggtct atttccttaa agtcaccacc tgttttattc tgtttttcag taagctggat
    10081 gaggcgcgca tcgatggact gcgtgacacg ctgcacgggt ggcgtttcat cccactggtc
    10141 gcgttttttc cacgcataaa tcgtgttcgg gtttattccc atcagacgtg atatttctgc
    10201 gggcggataa ccctgccagt aaagttgccg cgcacgctgg cgcacaaaag cgtcctgaat
    10261 cattgctccc cctgagtaat tacaggaaga ttacccgcgc gcgaaaccgt tctccttaac
    10321 cccctgttct ggccgttttc ttacaacaaa agccctttgt atcagcctgt tacgctttgc
    10381 catcatgact gaagaaccag tcagaggggc aaaaactatg gctaatgaaa aaaagacatc
    10441 ccgcaaaaag tttcgcgtgg ctgtctccgg atcaactgtt gatggccgtg aaatcagtcc
    10501 ggtgcatctg cgtgaagccg ccgagaactt caacccggat gtttacgctg cccgcgtgaa
    10561 cgttgagcac tatctctcgc catgcccgtc aagcgaattt tccgcaatgg gcgatgtcac
    10621 cgcactgagt acggaagaca ttacggaagg tccgctggcc ggacgtactg cgctgtatgc
    10681 agaaatcgaa ccgaccgagc gcatgaagca gcttgtcgcg gacggcaaga aaatctattc
    10741 cagtatcgaa ctgcacccgc agttctccgt taacgggcgc gcctatctgg tcgggctggc
    10801 gatgaccgac accccggcaa gcctgggcac tgagcgcctg aaattcacgg cacagcaacg
    10861 tcaggcggtg atgacgttca acagtatcca gggtgaagca ccgcttatct ccgaagccat
    10921 cgagtctgaa atcatcgaaa tggcagaaca acgccaggaa gaaggcaccc agtggtttaa
    10981 ccgcgtaatg gggattattg gtcgtggccg caaagcggat gacgccagtt tctcccgtat
    11041 tcaggaagcg gtggaaagcg tcgcaacgtc acaggccgac attatcgacc gttttaatgc
    11101 gctggaaaca cgccatcagc aggacagcca gaaaatcact ttactgacca cggagctgac
    11161 agcactgaag gaaaaactgc gcacgcagga cggcgatccg cagaaccggt tcaccgcaac
    11221 gggcgcagcc tccgaccagc tggctgactt ctgataagac aaaggagcaa attttttatg
    11281 aatctggtga tgtcagatat tacccgcaac aagctgggtt gctatatggc gcagcaggcg
    11341 tcgcttaaca atatcccggt ttctgcactg gtatcgcgat ttaccgtgga acctgcggtg
    11401 cagcaacgtt ttgaaaacgc ctcaaaggat agcaccgaac ttacaaaaaa aattaacgtg
    11461 atcggcgtga ccgaccagaa aggcgaaaaa atcctcctgg acaccacagg accgattgca
    11521 cgcacaaata ccagttatga cggaacaaaa cgccgtaacc cgaataacgt ggttgatctg
    11581 aaaaaccgca aataccagtg cgaacaggtg aactacgaca cgtttatttc gtatccgcag
    11641 cttgatgcct ggtcggcaca cagcgatttt cagtcccgca tcagcgcaca gattgcccga
    11701 caggtggcac ttgaccgcat catgatcggt ttcaacggca catctcacgc ggatgagtcc
    11761 aacttcagca ccaacaagct gcttcaggac gttaacgtgg ggtggctgga gcacatcaga
    11821 accaatgcca gcgagcgcgt aatgaatgac gtgacgctga cttcccgcaa catggacaac
    11881 accgtggcgc acgcgggtaa atacgcgaac cctgatgcac tggtacagga cgcgcgctca
    11941 tctctgctgg atgaatggca caaggaagct gacgacctcg tggtgattat ggggcgcaac
    12001 ctgtttaact cgctgcgtct gccagtgctg aacagcatca gcggccagaa tcccaatgcg
    12061 gaattacttg ccgggcagct catcctgtca tcgcgcacca ttggcgggct gggtgtgttc
    12121 cttgcaccgt tcttcccgga tgcaacgatg ctgatcacct cattcaacaa cctgtcgatt
    12181 tactggcaga a
//
LOCUS       DAZPEP010000121        11971 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZPEP010000121 DAZPEP010000000
VERSION     DAZPEP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.121"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            200..766
                     /locus_tag="ACHUT9_003188"
     CDS             200..766
                     /locus_tag="ACHUT9_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1349599.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            922..2954
                     /locus_tag="ACHUT9_003189"
                     /pseudo
     CDS             922..2954
                     /locus_tag="ACHUT9_003189"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            2931..3275
                     /locus_tag="ACHUT9_003190"
     CDS             2931..3275
                     /locus_tag="ACHUT9_003190"
                     /inference="COORDINATES: protein motif:HMM:NF025319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC C-terminal domain-containing protein"
                     /protein_id="HFR1349600.1"
                     /translation="MNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGKDVNVIIRNA
                     SGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQHCSLHLPEHM
                     EDTANRLILPCH"
     gene            3290..4021
                     /locus_tag="ACHUT9_003191"
     CDS             3290..4021
                     /locus_tag="ACHUT9_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1349601.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            4018..5079
                     /locus_tag="ACHUT9_003192"
     CDS             4018..5079
                     /locus_tag="ACHUT9_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1349602.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVTPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5231..6277
                     /locus_tag="ACHUT9_003193"
     CDS             5231..6277
                     /locus_tag="ACHUT9_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFR1349603.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6274..7065)
                     /gene="nei"
                     /locus_tag="ACHUT9_003194"
     CDS             complement(6274..7065)
                     /gene="nei"
                     /locus_tag="ACHUT9_003194"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFR1349604.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7101..7835)
                     /gene="pxpA"
                     /locus_tag="ACHUT9_003195"
     CDS             complement(7101..7835)
                     /gene="pxpA"
                     /locus_tag="ACHUT9_003195"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFR1349605.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7825..8757)
                     /gene="pxpC"
                     /locus_tag="ACHUT9_003196"
     CDS             complement(7825..8757)
                     /gene="pxpC"
                     /locus_tag="ACHUT9_003196"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFR1349606.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8751..9407)
                     /gene="pxpB"
                     /locus_tag="ACHUT9_003197"
     CDS             complement(8751..9407)
                     /gene="pxpB"
                     /locus_tag="ACHUT9_003197"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFR1349607.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9430..10173)
                     /gene="ybgI"
                     /locus_tag="ACHUT9_003198"
     CDS             complement(9430..10173)
                     /gene="ybgI"
                     /locus_tag="ACHUT9_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFR1349608.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10444..11925
                     /gene="dtpD"
                     /locus_tag="ACHUT9_003199"
     CDS             10444..11925
                     /gene="dtpD"
                     /locus_tag="ACHUT9_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFR1349609.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2892 a         3188 c         2903 g         2988 t
ORIGIN      
        1 tgaataaacc accaaatata gcgcacccct ataatttcca atactttatt catcaaacat
       61 ttaattcata ccatcaatta attactttta catttaataa aattaataat atttttaaga
      121 aaaactaaaa acattcgaca atcaaataag atattcttgc accgatatac ttttttcttt
      181 tctgttaaag gactcagtta tgtttaaagg acaaaaaaca ttggccgcac tggccgtatc
      241 tctgctgttc acagcacctg tttatgcggc tgatgaagga tccggtgaaa ttcactttaa
      301 aggtgaagtt attgaagcac cgtgtgaaat acatcaggat gatattgata aagaggttga
      361 actcggtcag gtgaccacca gccacattaa tcagtcacat cacagcgatg ccgttgctgt
      421 cgacctgcgc ttagtcaact gtgatctgga aaactccagc aacggttccg gtggcaagat
      481 ttctaaagtt gcagtcacct ttgatagctc agcgaaaacc accggcgcag atccgattct
      541 caacaacacc agcacgggtg aagccaccgg cgtcggcgta cgtttaatga ataaagatca
      601 aagcaatatc gttttaggca ccgctactcc agatatcgac ctggctccga cctccagcga
      661 acaaacgctg aatttctttg cctggatgga acaaattgat caggctacac ctgtaacgcc
      721 aggcgcagtt acggcgaatg caacctacgt gctggattat aaataagtat tactttggcg
      781 gcgctatcgc cgcctttcat aaaatattta tcaggtatgg acaccgtgaa tatttatcga
      841 ctctcttttg tatcctgcct ggtcgtggcg atgccttgcg cattggcggt cgaattcaac
      901 cttaatgttc tcgataaatc gatgcgcgac cgcattgata tttcattatt aaaggaaaaa
      961 ggagtcattg ctcccggtga gtattttgtt agcgttgcgg taaataataa ccaaatcagt
     1021 aacgggcaaa agattaactg gcacaaaaat gacgataaaa ccattccgtg catcaatgat
     1081 ttactggtcg ataaatttgg cttaaaacct gaagtccgtc agtcgttacc attgataaat
     1141 cagtgcgtcg attttagctc ccgacctgaa atgctcttca atttcgatca agccaatcag
     1201 caactgaata ttagtattcc gcaagcctgg ctggcgtggc actcagaaaa ctggaccccc
     1261 ccctccacat ggaaagaagg tgtcgccggt atcctgatgg attacaactt gtttgccagc
     1321 agctaccgcc cacaggacgg cagcagcagc actaacctga acgcctacgg taccaccgga
     1381 attaacgccg gggcatggcg cttacgtagt gattatcagt tgaatcagac tgatagcgat
     1441 gataaccatg aacagtcagg cggaatatcg cgcacctatc tttttcgtcc attaccgcaa
     1501 ttaggctcta aattaaccct cggcgaaacg gattttagtt ccaatatttt cgacggtttt
     1561 tcttataccg gcgcggcact ggcaagtgac gagcgaatgt tgccatggga actacgcggc
     1621 tacgccccac aaattagcgg tattgcacag accaatgcca cggtgacgat cagtcaatca
     1681 ggccgcgtca tttaccagaa aaaagtccca ccaggcccat ttatcattga cgaccttaat
     1741 cagtctgttc agggcacact ggatgtcaaa gtgacggaag aagatggtcg ggtgaacaat
     1801 ttccaggttt cggcagcatc gacgcccttc ctgactcgtc agggacaggt tcgctataaa
     1861 ctggccgcgg gtcagccacg gccctccatg tcacatcaaa ctgaaaatga aacctttttt
     1921 agcaatgaag tttcctgggg gatgctgtca aacacctcgc tgtacggcgg cctgctgctt
     1981 tctggtgatg actaccattc tgccgcaatg ggtattgggc aaaatatgct gtggcttggt
     2041 gcgctgtcgt ttgatgtcac gtgggccagt agccattttg atactcagca ggacgagcgg
     2101 ggcttaagct accgttttaa ttacagcaaa caagtggatg ccactaacag cacgatttcc
     2161 ctcgccgctt atcgtttctc cgatcgtcat tttcacagct acgccaacta tctggatcac
     2221 aaatacaacg acagcgatgc gcaggacgaa aaacagacga tcagcttatt gtgggccaac
     2281 cgattacccc actaaacctc aatctttacg ccaacctgct acatcaaacc tggtggaatg
     2341 cagacgcctc cacgaccgcc aacatcacgg caggttttaa tgttgatatt ggtgactgga
     2401 gagatatctc tatttcgacg tcattcaata caacccatta cgaagataaa gatcacgaca
     2461 accagattta cctgtcgatt tcgctcccct tcggtaacgg tggtcgggtt ggctatgaca
     2521 tgcaaaacag tagccacagc accacacacc gcatgtcgtg gaacgatacg ctggatgaac
     2581 gtaatagctg gggcatgtct gccggactgc aatccgaccg tcctgacaat ggagcccagg
     2641 tgagcggtaa ctatcagcac ctgagttcag cgggtgagtg ggatatttct ggtacctatg
     2701 ccgccaatga ttacagttcc gtcagcagca gctggagcgg ttctttcacc gcaacccaat
     2761 atggtgcagc gtttcatcgc cgcagctcca ccaatgaacc acgcctgatg gtcagcaccg
     2821 atggcgtggc agatattccg gttcagggca atctcgacta caccaaccat tttggcattg
     2881 cggtggtgcc gttgatttcc agttaccagc cttccaccgt ggcggtgaac atgaatgact
     2941 tacccgacgg cgtaacagtt gcagaaaacg ttatcaaaga aacgtggatt gaaggcgcga
     3001 taggttacaa atcactggct tcccgttccg gtaaagacgt taacgtcatc attcgcaacg
     3061 ccagcggtca gtttcctccc ctcggagcgg atatccgcca ggatgacagc ggcattagcg
     3121 tggggatggt tggcgaggaa ggacatgcct ggttaagcgg agtcgctgaa aatcaaaagt
     3181 ttaccgtggt ctggggtgat agccagcatt gctcgctcca tcttcctgaa catatggaag
     3241 acaccgcaaa tcgcctgatt ttaccttgtc attaattaag gaaatgaaaa tgacatttat
     3301 gaaaggacta ccccttttgt tgttggttgc cagtctgtgc agccacgctg cactacaacc
     3361 cgatcgcact cgtattgtgt ttaacgctaa tgataaagct accagtctgc gggtggataa
     3421 tcgtagcgat aaactcccct acctcgctta ttcctggctc gaaaatgaaa aaggggagaa
     3481 aagcgatgat cttctggtcg ccctgccgcc gatacaacgt ctggaaccga aagcaacaac
     3541 gcaggtacgg atagtaaagc aagcctcgac cacaaaatta ccgggcgatc gcgaaacgct
     3601 atttttctac aatatgcgtg aaattcctcc cgccccggaa aaaaacagcg accatgccgt
     3661 tctgcaagtc gccattcaga gtcgtatcaa ggtgttctgg cgaccggctg cattacgcaa
     3721 gaaagcagga gaaaaggtcg aactgcagtt acaggtcagc cagcagggca accaactgac
     3781 actgaaaaat cctaccgcgt attatctgac cattgcctac ctgggacgaa atgaaaaagg
     3841 cgttctcccc ggcttcaaaa ccgtgatggt tgcgcctttt agtacggtca acacaaacac
     3901 cggaaattat agcggcagcc agttttatct cggttatatg gatgattacg gcgcgttacg
     3961 catgacgacg ctcaactgca gcggacaatg ccatttacaa gccgtggagg cgaagaaatg
     4021 agtgctggca agggagtgtt gctcgtcatc tgtctgttat ttctgccgtt gaagtctgcg
     4081 cttgccctga actgctactt tggttcatcc ggtggctcgg tagaaaaatc agaagcgatt
     4141 caaccttttg ctgtaccagg caatgccaaa ctcggcgata agatttggga atctgacgat
     4201 attaaaattc ccgtctattg tgacaacaat accaacggca attttgaaag tgagcatgtt
     4261 tatgcctggg taacccccta cccgggggtg caggatcgct attatcaact cggcgtaact
     4321 tacaatggcg ttgattatga cgccaaccag ggaaaaagcc gcatcgacac taaccagtgt
     4381 attgacagta agaacattga catttatacc cctgagcaga ttatcgcgat gggatggcag
     4441 aacaaaattt gctcgggtga tcccgctaat attcacatgt cgcgtacctt tctcgctcgc
     4501 atgcggttat acgtcaaaat acgagaaatg ccgccgcatg attatcaaag tacgcttagc
     4561 gactatatcg tcgtgcaatt tgacggtgcc ggtagcgtta acgaagaccc tactgcccaa
     4621 aacctgaaat atcatattac tggtctggaa aacattcgcg tgctggattg cagcgtcaat
     4681 ttttccattt caccggaaac acaggtgatt gattttggta aatttaattt gctggatatt
     4741 cgtcgccaca caatgtcgaa aacattcagt attaagacga caaaatcaca aaatgaccaa
     4801 tgcaccgacg gatttaaagt cagttcctcc ttttataccg aagaaacgtt ggttgaagaa
     4861 gataaagcat tgttgattgg taatggtctg aagctacgtt tgctggatga aaacgcctcg
     4921 ccttatacct tcaataaata tgctgaatac gccgatttca ccagcgacat gttggtctac
     4981 gaaaaaacct atacagctga actttcgtcc attgcaggta cccccatcga ggctgggccc
     5041 ttcgatacag tggtgctttt taaaattaac tataactgat ttgccatgat gccctttgca
     5101 gccggaaagg gcatctcttt cagaaaattg ctgattttcc ccttttcctt gattgctgtt
     5161 attttttcct ggcccggctt tctttatgct gtatcgatac accaaagaga aaataatgag
     5221 ggagcgaagg atgccagttt tgcagtgggg aatgttatgt gtgctatcac ttctcctttc
     5281 tattggtttc ctcgcggtac atctcccggc ggcgctatta cttgggccga tgatcgccgg
     5341 gatcatcttc agtatgcgcg gaattactct gcaacttccc cgctccgctt ttctcgccgc
     5401 gcaggccatt cttggctgca tgattgcgca aaacctcact ggttcgattc tcaccacgct
     5461 ggccgccaac tggccgatcg tgcttgctat tttactggtg acgctgcttt ccagcgccat
     5521 cgtgggttgg ttattggtgc gctatagctc actgcctgga aataccggtg cctggggttc
     5581 ttctccaggc ggcgcagcgg caatggtcgc tatggcgcaa gattacggcg cagatattcg
     5641 cctggttgca tttatgcaat atctgcgagt gctgttcgtc gcgggggccg ctgttctggt
     5701 cacccgcatg atgctgggtg ataacgctga agcggttaac cagcaaatcg tctggttccc
     5761 gccagtgagc attaatctcc tgcttacgat tttgctcgca gtcgttgccg gtacggcggg
     5821 atgcatgctg cgtcttcctt ccggcacgat gctcatcccg atgctggcgg gcgcagtgct
     5881 ccagtctggt cagctcatca ccatcgaact accggaatgg ctgctggcga tggcgtatat
     5941 ggcaattggc tggcggattg gtcttggttt cgataaacaa atcttactgc gggcactacg
     6001 cccgctaccg caaatcctgc tgtcgatttt tgctctgctg gctatttgtg cgggtatggc
     6061 gtgggggctg acccggttta tgcacattga ttttatgact gcctacctcg ccaccagtcc
     6121 cggcgggctt gataccgtgg cggtcatcgc cgcagggagc aatgccgata tggcgcttat
     6181 tatggcgatg caaaccctgc gcctgttcag tattttgctg acggggccag ccattgcgcg
     6241 gtttatttca acctatgcgc cgaagcggtc ggcctagtgc tggcagccag ggcaccagta
     6301 aaacgggcga gatgacagcg tggttttctc aatgatgctg ccacagcgtt cgcacggttc
     6361 gccatcacga tgaaaaacct taaagcgaaa cagcgcccca tgatgcttat tctcatccac
     6421 ctgccccctc gtagcgtagg aaaatcgagg aatatccagt aacgcgtgtg cgagtgcatc
     6481 cagttgcgcc gcattaagat ctttcgcttt atgatttcca gtcaacccaa cctgccagag
     6541 gatctccacc cgcaaataat tgccaagccc agccagaaac gcctgatcga gcagtaatcc
     6601 tgcaaactga cggttacgaa agcgcggtga caataaccgt tcctttacca cctccggcgt
     6661 cagattcgga tccagcacat cgggaccaac gcgttgtaaa aacggatgcg tggtcagttg
     6721 ttccggcctc aacatctcaa tatccgaggc gctataaagc agaatggttt tgtcagccgt
     6781 ttgcagtttt acccgcaata ctcgcgtggt ctgcggctct tcgccggtat caaccacgcg
     6841 ccagacgccg taaagctgat tatggctgta gagcgttaag tcgttggaaa aatgagttaa
     6901 caacgcctta ccacgcgttt ccacatgggt aacgtgttga ccgataagtt gtgattgata
     6961 agtttttaac tgcgggaagg caaaccagac atcagttagt ggtttgcctt tgatcgccgc
     7021 ctccaggtta tccgctgcac ggcggatctc cgggccttca ggcatggtga tatccttttt
     7081 tcattcttta ttaatcgggg ttatgctgcg acaacaatct ccttttcggc aaatgtagag
     7141 cgtagtcggc gggcgaacgc cagtgcgtgc tcgccgtcgc catgcaggca gactgtttgc
     7201 gccgtgaccg ttgcccattc gccggtgata cttttgactc tgccgtgttg caccatctct
     7261 agcgtttgcg ccaacgcctg ttcttcgttt tcaatcaacg cgcctcgctg acttcgcggc
     7321 accagcgagc cgtcagcctg ataaccgcga tcggcaaaca cttcctcgcg cgttgtcagg
     7381 ccatattgct tgcctgcacg aatcagctcg cttcctgcca gcccaacgag aatcaatgcc
     7441 ggatcgcaag cgtataccgc tctggcgatg gcgtctgcca gttgtgcttc tttcgccgcc
     7501 tggttgtaca acatgccgtg cggtttgaca tgacgcatta cgccgccttg cgcacgggca
     7561 atcgttgcca gcgcgccaat ttgatacagc gtctgggcgt aaacggtttc tggcggcagc
     7621 tgcatggcgc tgcgaccaaa attttccctg tcgggaaaac tcgggtgagc gccaatcgcg
     7681 acaccatttt ttattgcttc acgcacgcaa gcctgcatga tttgcgcatc gcctgcatga
     7741 aatccacagg caatattggc agaggaaacc agcgttaata gctctgcgtc gctggcgcag
     7801 ccttcgccca gatcggcgtt caggtcaatt ttcattgtgc agccgccaca ctaattgttc
     7861 gaaataacgt tgctgatctt gccgcgcttt cagtgcctct tccagtgaac actggacaaa
     7921 atgaatcggc tgaccgagcg gaatttgcgc cagatggtac atatcagcct caatgataca
     7981 ggcaatacgc gggtaaccat cggtggtctg tgcgtcgttc atcaacacaa tcggctgccc
     8041 gttatgtggc acctgcacca cgcccggtaa caaaccgtga gataacagtt cgcgatcggt
     8101 ggtgcgtttt aaaatttgcc cctgtaagcg atagcccatg cggttacttt gcgagctaag
     8161 ctgccagggc gaacgccaga atgcatcctg cgaggcgcga tcgaactcat gatattccgg
     8221 ccccggcaag gcgcgaatgc ggttgcccca cagcagctgt ttaacgccct gcgcttccat
     8281 aaaatcacgc ttcgatttgc caatcgggag tcggtcacca tccttcagta aacggccttc
     8341 cagcccgcca atccccactt tgagatcggt gctgcatgac cccattaccg gcggaacatc
     8401 aataccaccc gcgaccgcca gataactgcg catcccgtgc tgcgggcgtt taagcgttaa
     8461 acgctggcct gctttcatcg gcaatcgcca gccggtccag acggcattat catccagccg
     8521 cgcttcgcaa ccggcacccg tcagagcaaa ccacccatca gtttcgaact caaccgttaa
     8581 ctgaccgagc gtgatctcca gcgcgggggc attggcgtca ttacccacca gcaggttagc
     8641 aatgcgtaac gcaggcatat ccagtgcgcc gcagtggctg attcccgact ggcgaaaacc
     8701 gtgacgaccg ccatcctgca cagtggtata catgcccgca cgaataatct tcagcatact
     8761 ccctccttct gcggtacaaa gcgcacgctg tctcccggac gtaataagat gggttcgtca
     8821 cgcgccggat caaacaggct gagtgaggta tgaccaatca actgccagcc acccggcgtt
     8881 gccagcggat aaacaccagt ctgcggcccg ccgatcccga cagaacctgc cggaacgagc
     8941 aagcgcggtt cagcgcgccg tggcgtgtgt agttgttccg gcaaactccc gagatacggg
     9001 aagcccggtt gaaaacctaa aaaccagacc acgtattcca cggaggagtg caattcaaca
     9061 acctgttttt cgctaaaccc gcaatgcgcc gcgaccaccg ccaaatccgg tccgcctgca
     9121 ccaccgtaaa ccaccggaat ttcaataaag cgagactcag gttccagggc ctcgctctcc
     9181 tcccaccagc gttgcaaacg ctctatggca tccagcgcca gcgactcagg attacgcaaa
     9241 atcaccgtga tattgttcat gccgggaatg gcttcaacca cattcggcat atccaccaga
     9301 cgctgcgcca gtcgccagat ccgtttctgg ctagccagcg tcaccggcgg ttccagttcc
     9361 agcactaccg ccgtttcacc tatcagataa caacgcgctc gttgcacttt cgatccctct
     9421 gattattcgt tatgcaggat taggaatatc aataaaggtc acatcaagat cggtattttc
     9481 attcagccac tcgctcaatg cgcgaatacc accacgttca gtggcatggt gacctgcagc
     9541 ataaaaatgc aacccctgct cgcgggctga atgaatggtc tgttcagaaa cttcgccagt
     9601 aataaaagca tccacgccaa aacgcgcggc gctatcgata aaactttgcc cgccgcccgt
     9661 gcaccaggcg acgcgctgga ccacctcagg tccggtatcg ccacaccata acggcttacg
     9721 tcccagacgc gcttcaatcc aggaggctag ttccagtccc ggcacaggca tggtcagctc
     9781 tccccacggc accaacggct caatttcgcc catgaccgtg atccccagta acgccgccag
     9841 ctgtgcgtta ttgcccagct caggatgtac gtcaagcggc aaatgccagc catacaggtt
     9901 gatatcattc gccagcaacg tttttaaacg gttacgcttc attccgcgaa tgaccggaga
     9961 ctctcctttc cagaagtagc catgatgcac aatgaccgca tccgcgccca gacgcactgc
    10021 ctcatcgagc aaagcctggc tggcggtgac accggtaaca attttttgca ccgtctcttt
    10081 gccttctacc tgcaaaccat tcggcgcgta atcactaatc gccgcgctgt tcagtttttc
    10141 gttaatcagt tgttccagtt cggtgttttt catcttcgcg ccttccgtat tctgatagct
    10201 acaccgttaa catagcgtta cctcgcctgt tcgtcgatag ccaatctttt gacatattaa
    10261 gaaacaaacc cgacgccaaa agacgcaaat tgtttatatc atcacgcttc agctaatgta
    10321 tatttatgca atcagacagc atattgcata aataatcaga tctgtatcaa agttttaact
    10381 ttaaggaaac acaagcacgc cgcgcaacta ttacgtgacg ttgcagtcaa ttgtggaatt
    10441 attatgaata aacacgcatc acaaccgcgc actatttact atgtcgttgc gctgcaaatc
    10501 tgggaatatt ttagctttta cggcatgcgt gccctgctga ttctctatct caccaatcaa
    10561 ctaaaataca acgatactca cgcctacgag ttatttagcg cctactgttc gctggtgtat
    10621 gtcacgccaa tcctcggtgg ctttttggcg gataaagttc tcggcaatcg catggcggtg
    10681 atgctggggg cgttgttgat ggcgatcggt catgtggtgc tgggtgccag tgagatccat
    10741 ccgtcattcc tctatctgtc cctggcgatt atcgtttgtg gctatggtct gtttaaatcc
    10801 aatgtcagtt gcctgctcgg cgagctgtat gagccaaccg atccgcgtcg tgatggcggt
    10861 ttctcgctga tgtatgcagc gggtaacgtg gggtctatta tcgcacctat cgcctgtggt
    10921 tacgcccagg aagagtacag ctgggcgatg ggctttggcc tggcggcggt gggtatgatc
    10981 gcgggtctgg tcattttctt atgtggcaat cgtcatttca cacatacccg cggcgttaat
    11041 aaaaaagtac tgcgtgcaac aaactttctc ctgcctaact ggggatggct gctggttctg
    11101 ctggtggcaa cgcctgcgct gattaccgta ctgttctgga aagagtggtc ggtatacgcc
    11161 ttaattgtcg cgactattat tggcctgggt gtactggcaa aaatttatcg caaagcagaa
    11221 aaccagaaac agcgtaagga gctggggctg attgtgacgc tcaccttctt cagtatgttg
    11281 ttctgggcct tcgcacaaca gggcggcagc tcaattagcc tttatatcga ccgcttcgtt
    11341 aaccgcgata tgtttggtta taccgttccg actgctatgt tccagtcgat taatgccttc
    11401 gcagttatgc tatgcggtgt gttcctggcg tgggtggtta aagaaagcgt cgcaggtaat
    11461 cgtaccgtgc gcatctgggg aaaatttgct cttggccttg gcctgatgag cgccggattc
    11521 tgcattctga ccttaagcgc ccgctggtcc gcaatgtatg gtcactcttc tctgccactg
    11581 atggtgttag gcctcgcggt gatgggcttt gcggaactgt ttatcgaccc ggttgccatg
    11641 tcgcaaatta cgcgcattga aattccgggc gtgaccggcg tattaaccgg catctacatg
    11701 ctgctttctg gcgcgatcgc gaactatctg gcgggcgtga ttgccgatca gacatcgcag
    11761 gcttcgtttg atgcttccgg ggcgatcaac tactccatca atgcatatat tgaagtattt
    11821 gatcaaatta cctggggcgc actggcgtgt gtaggactgg tactgatgat ttggctgtat
    11881 caggcgctga aattcagaaa ccgcgcgctg gcgctggagt cataataggc cagcaccgat
    11941 gcctgatgcg acgcttgacg cgtcttatca g
//
LOCUS       DAZPEP010000122        11925 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZPEP010000122 DAZPEP010000000
VERSION     DAZPEP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11925
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.122"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUT9_003200"
     CDS             complement(<1..914)
                     /gene="ydcO"
                     /locus_tag="ACHUT9_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFR1349610.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLLAGLFG"
     gene            1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUT9_003201"
     CDS             1006..1542
                     /gene="sutR"
                     /locus_tag="ACHUT9_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFR1349611.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUT9_003202"
     CDS             1615..3576
                     /gene="rlhA"
                     /locus_tag="ACHUT9_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFR1349612.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3668..3898)
                     /locus_tag="ACHUT9_003203"
     CDS             complement(3668..3898)
                     /locus_tag="ACHUT9_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFR1349613.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4120..4274
                     /locus_tag="ACHUT9_003204"
                     /pseudo
     CDS             4120..4274
                     /locus_tag="ACHUT9_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            4320..4736
                     /gene="hicB"
                     /locus_tag="ACHUT9_003205"
     CDS             4320..4736
                     /gene="hicB"
                     /locus_tag="ACHUT9_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFR1349614.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            4815..6221
                     /locus_tag="ACHUT9_003206"
                     /pseudo
     CDS             4815..6221
                     /locus_tag="ACHUT9_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHUT9_003207"
     CDS             6466..7611
                     /gene="ydcS"
                     /locus_tag="ACHUT9_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFR1349615.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7629..8642
                     /locus_tag="ACHUT9_003208"
     CDS             7629..8642
                     /locus_tag="ACHUT9_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1349616.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8643..9543
                     /locus_tag="ACHUT9_003209"
                     /pseudo
     CDS             8643..9543
                     /locus_tag="ACHUT9_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            9533..10327
                     /locus_tag="ACHUT9_003210"
     CDS             9533..10327
                     /locus_tag="ACHUT9_003210"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFR1349617.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10349..11773
                     /gene="patD"
                     /locus_tag="ACHUT9_003211"
     CDS             10349..11773
                     /gene="patD"
                     /locus_tag="ACHUT9_003211"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFR1349618.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2879 a         2896 c         3200 g         2950 t
ORIGIN      
        1 ccaaacagac ctgcgagcaa atagaaaatg cctgcaacgg cagctgccag ccaacgttga
       61 tctttatccg gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg
      121 acggaataaa cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat
      181 ggcgaaacag gagccgaata tccagctgct ttcattgctg cgatacccgg tgcgttttgc
      241 gatgccatcg tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa
      301 tcaggggtaa tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca
      361 ccttgcgcga taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc
      421 gcaacggcct tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg
      481 tccagactgg caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc
      541 gcaagcgagt gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg
      601 actattaacg cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc
      661 aatccggtga ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga
      721 taccataatg tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag
      781 ccagagattt gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct
      841 gaactggcgt agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata
      901 gaaaacagac gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg
      961 ttataacgca caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct
     1021 cgctttttat ccactacact taaacaacta cgccagcagc gcggctggag tctttcgcga
     1081 ctggcagaag cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc
     1141 agtccgacgg tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca
     1201 tttatttctc cgccgcagtc cgctatgcct tcagtttatg atccacaaca gcaggcgatg
     1261 gtgatcactt cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag
     1321 atggcatctg gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc
     1381 gtcgtcattg atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt
     1441 ggggaaggcg ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa
     1501 accgtacatt ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc
     1561 aacgtcgcgc ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc
     1621 gtatcttctc atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa
     1681 gctattttgc acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat
     1741 aatgccagta atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt
     1801 gcaaaaattt tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa
     1861 cggctgatta ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg
     1921 attctggaac ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt
     1981 acagttgaaa aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga
     2041 gagctgaatc ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc
     2101 tttattcatg gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa
     2161 acagggcgta gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg
     2221 aaagacgatc aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac
     2281 gatcagactg ctaacctcgg cgcgctgatt gatgctggcg tacgctcctt caagattgaa
     2341 gggcgttaca aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctg
     2401 gatgccatta ttgaagaacg tggcgatctg acgcgtgctt catcaggtcg tactgaacat
     2461 ttctttgttc catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat
     2521 gcccgtaaag gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtgggt
     2581 gaagtattga aagtggcgaa agatcatctc aatgttgccg tcaccgagcc actggcaaat
     2641 ggtgatggcc tgaacgtgat gattaaacgt gaagtcatcg gttttcgtgc caatacggtc
     2701 gagaaaaccg gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac
     2761 aaaattcgtc cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg
     2821 acaaaaacct ctagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa
     2881 caactgattc tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg
     2941 cagttcgacg aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca
     3001 aaactggggc aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg
     3061 tttgtaccaa acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct
     3121 gcgcgtcttg ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt
     3181 tttccgcaaa cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt
     3241 tatcatcgct atggtgtgca gctgattgac gcggcgtatg aggcacatga agagaagggc
     3301 gaagtcccgg tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa
     3361 caggcgaaag gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat
     3421 gaagtattaa cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc
     3481 aaaaatcaca tactgaaaat gccgttaccg ggaagcgtag tggcatccgt aagtccggat
     3541 gagctgctga aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac
     3601 aactcattga atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcatc
     3661 aaacgactca acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc
     3721 gcaaaccttc cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac
     3781 gcagctgatg atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga
     3841 gttgtcctga gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc
     3901 tgccaccttt ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc
     3961 agttttgcaa attagcgcaa agaaattctg gaatcttcct tcctgatttt gcattgcatt
     4021 ctgccgttgc ggcgatttag tgctattttc gagcaaatta cacatagggg taaacactaa
     4081 tgtttacttt attgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga
     4141 cgttggctcg aatctcaggg cgtcgatgta gcgaatggcc gcaaccattt gaaactcagg
     4201 tttcatggga ggcgcagtgt catgccgcgt cactcctgcg taaagcaatc ctgaaacaac
     4261 tcggtttgag ttaatcgcca attaaaaagg ttaatgacat gcgagagaca gtcgaaatta
     4321 tgcgttatcc cgtcactctt acacccgcgc cggaaggcgg ttatatggtt tcttttgtgg
     4381 atatccctga agcgttgacg cagggcgaaa ctgtcgctga agcgatggaa gcggcaaaag
     4441 atgctttact gaccgcattt gatttttatt ttgaagataa cgagcttatc ccgttacctt
     4501 cgccattaaa tagtcatgat cactttattg aagtaccttt gagcgtcgcc tctaaagtat
     4561 tgctgttaaa tgctttttta cagtcagaaa tcactcagca agagttagcc aggcgaattg
     4621 gcaaacctaa acaggagatt actcgcctat ttaacttgca ccatgcgaca aaaatcgacg
     4681 ccgtccagct tgcggcaaag gcgcttggca aagagttatc gctggtgatg gtttaattac
     4741 agttaacgaa aagttgtcat ttttaacaac tgatatagac tgccgaatca tctgcacata
     4801 attacgattc gataatgaaa aaataccagc agcttgcaga acaattacgc gagcagattg
     4861 cgtcgggtat ctggcaaccc ggcgatcgtt tgccttcgtt gcgtgaccag gtggcgcttt
     4921 caggcatgag ctttatgact gtcagccatg cctatcagtt gctcgaaagt cagggatata
     4981 ttatcgcacg accgcagtcg ggttattacg ttgcgccaca ggcaataaaa atgccgaaag
     5041 cgccagtcat tccagtcact tgagatgaag cagtcgatat caacacttat atttttgata
     5101 tgttgcaggc cagtcgcgaa ccgtcggtcg ttccgtttgc ctcggccttt cccgacccgc
     5161 gacttttccc cctccaacaa ctaaaccgct cgctggcgca ggtaagcaaa accgccacag
     5221 cgatgagcgt gattgaaaac ttaccgccag gaaacgcaga actgcgtcag gctattgctc
     5281 gtcgctatgc cttacagggc atcaccattt ctcctgatga aattgtcatt actgccgggg
     5341 cgttagaggc attaaacctc agtttgcaag cggtaactga accgggcgat tgggtgatag
     5401 tagagaatcc ttgtttctac ggtgcgttgc aggcgctgga gcggctacgg ctgaaggcgt
     5461 tatcggtggc gacggatgtt aaagaaggga tcgatcttca ggcgctggaa ctggcgttgc
     5521 aggattatcc ggtgaaagcg tgctggctga tgactaatag ccagaaccca ctcggattta
     5581 ccttaacgcc gcaaaaaaaa gcacaactgg tggcgttact caatcagtac aacgtaacgc
     5641 tgattgaaga tgacgtttac agcgaacttt attttggacg ggaaaaaccg ctgcctgcga
     5701 aagcgtggga tcgccacgat ggcgttttgc attgctcttc gttttcgaaa tgtctggtgc
     5761 ctggttttcg tattggttgg gtcgccgccg gaaaacatgc acgtaaaatt caacgcttgc
     5821 agttgatgag tacgctttcc accagctcac cgatgcaact tgcgctggtg gattaccttt
     5881 ccacgcgccg atacgacgcc catcttcgtc gcctgcgtcg ccagcttgcg gaacgtaaac
     5941 aacgtgcctg gcaggcactg ctgcgttatc tgcctgcgga agttaaaatt catcatagtg
     6001 acagcggtta ttttctctgg ctggagctcc ccgagccgtt agatgccggc gaattaagcc
     6061 tggcggcact gacgcatcat atcagtattg cgccgggtaa aatgttttct accggtgaaa
     6121 actggtcacg tttttttcgt tttaataccg cgtggcagtg gggagaacgt gaagaacagg
     6181 cggtaaaaca attaggcaaa cttattcaag aacggctgta atagcgttta atttaattcc
     6241 tcttagattg ggtaatatga atttcgaata gcagtcatat ttcctaaccc cttgtctata
     6301 ctccagaaga taaccttaca gacggcataa tgcgcggtag ctcacaacct gattaaattt
     6361 tctcagggac gaaggtgtgc ctgcaagccg ccgtctatgg ttaaacaagg agatattttt
     6421 acggcacggc ggctgaacaa ttaattacga caggagtaag accttatgag caagacattt
     6481 gcccgcagca gcctgtgtgc gctcaccatg acaataatga ccgctcacgc cgccgaacca
     6541 cctaccaatt tagataaacc ggaagggcga ctggatatta tcgcctggcc gggatacatc
     6601 gaacgcggac aaactgataa acaatacgac tgggtaacgc aatttgaaaa agagacaggc
     6661 tgcgcggtga atgtgaaaac cgccgcgact tccgatgaaa tggtcagtct gatgaccaaa
     6721 gggggttacg atctggttac ggcatccggc gatgcctcgc tgcgtttgat tatgggcaaa
     6781 cgcgtgcagc cgattaatac cgccttgatt cccaactgga aaacgctcga tccgcgcgtg
     6841 gttaaaggcg actggtttaa tgttggcggc aaagtttacg gcacacctta ccaatggggg
     6901 ccgaacctgc tgatgtacaa cactaaaacc ttcccgacgc cgccggatag ctggcaagtg
     6961 gtttttgttg agcaaaatct gccggacggc aagagcaata aaggccgcgt tcaggcttat
     7021 gatggcccta tctacattgc ggacgctgcg ttgttcgtta aagccactca gccgcagttg
     7081 ggcatcagcg atccgtatca actaaccgaa gaacagtacc aggcggtgct gaaagtgctg
     7141 cgcgctcaac acagtttgat ccatcgctac tggcatgaca ctaccgtgca aatgagcgat
     7201 ttcaaaaacg agggtgtggt tgcttccagt gcctggccct atcaggccaa cgccctgaaa
     7261 gccgaaggcc agcctgttgc taccgttttc ccgaaggagg gtgttaccgg ttgggctgat
     7321 accaccatgc tgcatagcga agcgaaacat ccggtttgcg cctacaaatg gatgaactgg
     7381 tcattaacgc caaaagtgca gggcgatgtg gcggcctggt ttggctcgtt accggtagtg
     7441 ccggaagggt gtaaagccag tccgttatta ggcgaaaaag gttgtgaaac caacggtttt
     7501 aactatttcg acaaaatcgc cttctggaaa acgcctatag cagaaggggg caagtttgtt
     7561 ccctacagtc gctggacgca ggattacatt gccattatgg gcggtcgcta acttcgctgg
     7621 ggtgctttat gacgtacgca gtggagtttg acaacgtctc gcggttgtac ggtgacgtgc
     7681 gcgcagtaga tggcgtcagt attgcgataa aagatggtga gttcttctct atgctggggc
     7741 cgtccggctc cggcaaaacc acctgcctgc gcctgattgc tggcttcgaa cagctttccg
     7801 gcggggctat ctctatcttt ggtaaacccg ccagcaatct gccaccgtgg gagcgggacg
     7861 tgaatactgt ctttcaggac tacgcgctat ttccgcatat gtcgattctt gacaatgtcg
     7921 cctatgggct gatggtcaaa ggcgtgaata aaaagcagcg gcacgcaatg gcgcaagagg
     7981 cgctggagaa agtggcgttg gggtttgtac agcaacgtaa accgtcacaa ctttctggtg
     8041 gtcagcgcca gcgggttgct atcgccagag cattggtgaa tgaaccgcgc gtattgctgt
     8101 tggatgaacc gctcggcgca ctggatctca aattgcgtga gcagatgcag ctggaactga
     8161 aaaaactgca acagtctctc ggtatcactt ttatcttcgt tacccacgat cagggcgaag
     8221 cgttatcgat gtccgatcgt gtggcggttt tcaataatgg acgcattgag caggtcgatt
     8281 ccccgcgcga tctctatatg cgcccgcgca cgccgtttgt tgccgggttc gttggtacat
     8341 cgaatgtttt tgatggactg atggcagaga aactttgtgg catgacggga agcttcgccc
     8401 tgcgaccgga acatatccgc ctcaacaccc ctggtgaact gcaggccaat ggcacgatcc
     8461 aggcggtgca atatcagggc gcggcaactc gttttgaact gaaattgaac ggcggtgaaa
     8521 aactgcttgt gagtcaggcc aatatgacag gcgaagaact gcctgccacg ctcacgcccg
     8581 gacaacaggt gatggtttcc tggtcgcgtg atgtgatggt gccgctggtt gaggagaggt
     8641 gaatggcgat gaatgtattg caatcacctt cacgtccagg tctggggctg tttttactgc
     8701 tgcttggccc gctaatgtgg tttggcattg tctatttcgg ctcgctgctg acactgttat
     8761 ggcagggatt ttatactttt gacgatttca ccatgtcggt aacgccggaa ctgacgctgg
     8821 cgaatatccg tgcgctgttt aatccggcga attacgacat cattctccgc acgctgacta
     8881 tggctgtggc agtcactatc gccagcgcca ttctggcttt tccaatggcg tggtatatgg
     8941 cgcgctatac cagcgggaaa atgaaagcgt ttttatattg cggtaatgtt gccgatgtgg
     9001 gcgagctaca ttgttaaagc ctatgcctgg acgttattgc tggcaaaaga tggcgtggct
     9061 cagtggtttt tacaacatct tgggctggaa ccactgctga ctgcgttcct tacattacct
     9121 gcggtgggcg gaaatacgct gtcaacttcc gggctggggc gctttctggt gtttctctat
     9181 atctggttgc cgttcatgat cctgcccgtt caggcggcgc ttgagcgttt gccgccgtca
     9241 ttgttgcagg cgtcggctga tctcggcgca cgtccacgac aaacctttcg ctatgtggtg
     9301 ctgccgctgg caatcccggg tattgccgct ggctctatct ttaccttctc actcacactg
     9361 ggcgatttta tcgtcccgca gctggttggt cctccaggat attttatcgg caatatggtt
     9421 tattcccagc agggggcgat tggcaatatg ccgatggcgg cggcattcac cctggtgccg
     9481 attattctca tcgcactgta cctggcgttc gtgaaacgtc tgggagcgtt cgatgcactc
     9541 tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc ggcgttgttt tcctacattt
     9601 tcccatcctg ataatcgccg cctatgcgtt taacactgaa gatgcggcgt ttagttttcc
     9661 accgcagggc ctgacgctgc gctggtttag cgtggcagca cagcgtagtg atattcttga
     9721 tgccgtgaca ctgtcactta aagtggcggc gctggcgaca ttaattgcgc tggtgttagg
     9781 gacgctggca gctgccgcgc tgtggcgacg agactttttc ggcaaaaacg ccatttcgct
     9841 gttactgctg ctgcccattg cgctgccggg cagcgtcact ggtctggcgt tattaaccgc
     9901 ctttaaaacc atcaatctgg agccgggatt tttcaccatc gtggtcggtc atgcgacttt
     9961 ttgtgtagtt gtggtgttta acaatgtcat cgcccgtttt cgccgcacct cctggagtct
    10021 ggttgaggcg tcaatggatc ttggggccaa tggctggcaa accttccgct acgtagtgtt
    10081 gccgaatctc agttcggcgt tactggcagg aggaatgctg gcgtttgcct tgtcgttcga
    10141 tgaaatcatc gttacgacct ttacggcagg tcatgaacga acgttactgt tgtggttgct
    10201 caatcagctt gggcgaccgc gtgatgtacc ggtaactaac gtggtggcac tgctggttat
    10261 gttggtaaca accttgccga tcctgggggc ctggtggcta acccgcgaag gcgacaatgg
    10321 tcaataacca ctgatacagg aatatgctat gcaacataag ttactgatta acggagaact
    10381 ggttagcggc gaaggggaaa aacagcctgt ctataatccg gcaacggggg acgttttact
    10441 ggaaattgcc gaggcatccg cagagcaggt cgatgctgct gtgcgcgcgg cagatgcagc
    10501 atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg gaatgtctgc tgaaactggc
    10561 tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg gagtcccgta attgtggcaa
    10621 accgctgcat agtgcgttca atgatgaaat cccggcgatt gtcgatgttt ttcgcttttt
    10681 cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt gaatatcttg aaggtcatac
    10741 ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct atcgcaccgt ggaattatcc
    10801 gctgatgatg gccgcgtgga aacttgctcc ggcgctggcg gcagggaact gcgtagtgct
    10861 taaaccatca gaaattaccc cgctgaccgc gttgaagttg gcagagctgg cgaaagatat
    10921 cttcccggca ggcgtgatta acatactgtt tggcagaggc aaaacggtgg gtgatccgct
    10981 gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc tctatcgcca ccggcgagca
    11041 catcatcagc cataccgcgt cgtccattaa gcgtactcat atggaacttg gtggcaaagc
    11101 gccagtgatt gtttttgatg atgcggatat tgaagcagtg gtcgaaggtg tacgtacatt
    11161 tggctattac aatgctggac aggattgtac tgcggcttgt cggatctacg cgcaaaaagg
    11221 catttacgat acgctggtgg aaaaactggg tgctgcggtg gcaacgttaa aatctggtgc
    11281 gccggatgac gagtctacgg agcttggacc tttaagctcg ctggcgcatc tcgaacgcgt
    11341 cagcaaggca gtagaagagg cgaaagcgac agggcacatc aaagtgatca ctggcggtga
    11401 aaagcgcaag ggtaatggct attactatgc gccgacgctg ctggctggcg cattacagga
    11461 cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg agtgttacgc ccttcgacaa
    11521 cgaagaacag gtggtgaact gggcgaatga cagccagtac ggacttgcat cttcggtatg
    11581 gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg ctgcaatatg gttgtacctg
    11641 ggtcaatacc catttcatgc tggtaagtga aatgccgcac ggtgggcaga aactttctgg
    11701 ttacggcaag gatatgtcac tttatgggct ggaggattac accgtcgtcc gccacgtcat
    11761 ggttaaacat taagttagat gttagtggct gttataggac ggccactatc gttttaggtt
    11821 ctgagtttaa tggcggttaa gtattagaat gattacatta ataactagcg taaaaattag
    11881 gctaaatgtt tgatggcgat ttttcatcct gtcacagttg attag
//
LOCUS       DAZPEP010000123        11917 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZPEP010000123 DAZPEP010000000
VERSION     DAZPEP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11917
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.123"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            29..790
                     /gene="ydeO"
                     /locus_tag="ACHUT9_003212"
     CDS             29..790
                     /gene="ydeO"
                     /locus_tag="ACHUT9_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFR1349619.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMSALAI"
     gene            1191..2873
                     /locus_tag="ACHUT9_003213"
     CDS             1191..2873
                     /locus_tag="ACHUT9_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFR1349620.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            2925..4082
                     /locus_tag="ACHUT9_003214"
     CDS             2925..4082
                     /locus_tag="ACHUT9_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFR1349621.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(4048..4170)
                     /locus_tag="ACHUT9_003215"
     CDS             complement(4048..4170)
                     /locus_tag="ACHUT9_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349622.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESIINYSPFVLQHP"
     gene            4379..6064
                     /locus_tag="ACHUT9_003216"
     CDS             4379..6064
                     /locus_tag="ACHUT9_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFR1349623.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
     gene            6102..8474
                     /locus_tag="ACHUT9_003217"
     CDS             6102..8474
                     /locus_tag="ACHUT9_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1349624.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMVEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            8531..11314
                     /locus_tag="ACHUT9_003218"
     CDS             8531..11314
                     /locus_tag="ACHUT9_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFR1349625.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            11676..>11917
                     /locus_tag="ACHUT9_003219"
     CDS             11676..>11917
                     /locus_tag="ACHUT9_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1349626.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         3547 a         2576 c         2534 g         3260 t
ORIGIN      
        1 aaaaacttta ttgttttaag gagataaaat gtcgctcgtt tgttctgtta tatttattca
       61 tcatgccttc aacgctaaca ttttagataa agattacgcc ttctctgacg gcgagatcct
      121 gatggtagat aacgctgttc gtacgcattt tgaaccttat gagcggcatt ttaaagagat
      181 cggatttact gaaaatacca ttaaaaaata tctacaatgc actaacatcc agacagtgac
      241 ggtgcctgtt cctgcgaagt ttttacgtgc ttcaaatgta ccgactggat tgcttaatga
      301 aatgattgct tatcttaact cggaagaacg caatcatcat aatttttcag aacttttgct
      361 tttttcttgc ctgtctattt ttgccgcatg caaaggtttc attacactat taactaacgg
      421 tgtgctatcc gtttccggaa aagtgagaaa tattgtcaac atgaagctgg cgcacccatg
      481 gaagctgaaa gatatttgtg actgcctgta catcagtgaa agcctgttga agaaaaaact
      541 aaagcaagag caaacgacat tctcacagat tctattagat gccagaatgc agcacgcaaa
      601 aaatttgata cgcgtagaag gttcagtcaa taaaattgcc gaacaatgtg gttatgccag
      661 tacatcttat tttatttatg cgttccgcaa acatttcggc aacagtccga agagagtttc
      721 taaggagtac cgttgtcaaa gtcacatggg tatgaatacg ggcaacacga tgagtgcttt
      781 agctatttga ttatttgcta acgagtagtc aaccacgcac gctgcgtaag aattaaatgg
      841 ggcagccatt ccctgccccg cgttgttttt aggcgatata tttattgaaa taaataagtg
      901 acatccatca catatttatg cacttgcata acctgttgca gattatttat gatctcaatt
      961 cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg
     1021 gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc
     1081 tttttttagc taaatctgct atctctttat gtgactaact tcacttacat ccacttattt
     1141 ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg
     1201 cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg
     1261 catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt
     1321 tctcagattt tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa
     1381 ccatggatga tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa
     1441 caatggaaaa tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag
     1501 ctgcacaaaa atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta
     1561 acggctatgt ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag
     1621 ctcccgcccg ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag
     1681 aaactttctt gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat
     1741 ggcacttgtc aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc
     1801 atgacaactt caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt
     1861 actttatggg attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa
     1921 atcgtgaacg tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg
     1981 gcgttgttga tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg
     2041 ctccgcacct accaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg
     2101 gtagtcaaac agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac
     2161 gcattctcga acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct
     2221 ccgataatgg tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata
     2281 agagtcagac ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac
     2341 ttcaacccgg taattatgac aagctgattt ccgcaatgga tttctaccca acagctcttg
     2401 atgcagccga tatcagcatt ccaaaagacc ttaagctgga tggtgtttcc ttgctgccct
     2461 ggttgcaaga taagaaacaa ggcgcgccac ataaaaatct gacctggata acctcttatt
     2521 ctcactggtt tgatgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc
     2581 atcagtcaga cgattacccg cataacccca acactgagga cttaagccaa ttctcttata
     2641 cggtgagaaa taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct
     2701 acaaactgac ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta
     2761 aagagatgca aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgagg
     2821 taaatcagga gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc
     2881 ttcatgcggc ggatttttcc gccgccttat tgagcgagat agcgatgcac gttacagcca
     2941 agccctccag ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt
     3001 cgcagtttac tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat
     3061 atatcgaagc gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg
     3121 ctggcctgga ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac
     3181 gtatttttaa tgcattacaa acgaatggca ttttattgaa taatgaatgg tgtgcctttc
     3241 tcaaagaaca tgaatttctg gttggtatct cgatcgatgg cccccaggag ttacatgacc
     3301 gttacagacg cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc
     3361 gtctgaaatc atatcaagta gagtttaata cgttaaccgt cattaataac gttaatgtcc
     3421 attaccctct tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta
     3481 tcgaattgct agaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat
     3541 tccgtatcat tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt
     3601 ttatgcaatg ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg
     3661 tcagccgttt tttggggaat gggcatacaa gttgtatttt ccaggagtcc tgcaaggata
     3721 atctggttgt tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt
     3781 acaaaattgg aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag
     3841 cgcaaaaaaa acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg
     3901 gcggttgtcc taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg
     3961 aaggttataa aatccttttt tcaaccatgg taccttatat gaacgccatg gttgagttag
     4021 ctaagaacag agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt
     4081 aataatgctc tcttttaact ccattcttag cctccttctt atgaccttaa caaggcaccc
     4141 gacataaatt cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt
     4201 acgtggtatg cgtcagggca ttagcaatac ccttgattat caatagcata accataacca
     4261 taaccactga aaattaaaaa tttctatata tttcatattg ttaaatgata cttaaataaa
     4321 tttcatcagt aaatcattgc catagataat cattggatgg cacaatcaat tttcgctaat
     4381 gataaccatt cccattacgt tttatatgtt gatagcaaag tatctctgtc tgctgaagcc
     4441 cttttggctg cgtaagaata ataaaacttc agtgttgtta atcattatta tcctcgcaat
     4501 gatccttggc gtggttaaaa ttcaggtctg gttaaacgac tggaataatg attttttcaa
     4561 tgcgctaagc cagaaagaaa ccgataagct ttggcagctt gttctttggt tccctgccct
     4621 gctggggatc tttgtattaa tatccgtaaa caaaacatgg ttaattaaac tactgactat
     4681 tcgctggcgc gaatggttaa cagattacta ccttaatcgc tggtttgcag ataaaaacta
     4741 ctacttcacg cagatctacg gcgagcataa aaatacagat aaccctgacc aacgtatcgc
     4801 tgaagacatt ctcttactga tcagcaaaac gctcagtctt tcatttggct tcatccagtc
     4861 acttagcatg ctgatcacct ttaccgttat tctttggcaa agcgcgggta cgctctcatt
     4921 cactgttggt ggaacagaat ggaatatcca ggggtatatg gtctataccg tcgtgctcat
     4981 tgtgatcggt ggaactttat ttacccataa ggtgggtaaa cgtattcgtc cgcttaatgt
     5041 ggaaaaacaa cgtagcgaag cgacatttcg aactaatctt gtgcagcata ataagcagac
     5101 agaactgatt gcgctaagca atgctgagtc tctacaacgc caggagttgc gcgacaattt
     5161 tcataccatt aaagagaact ggcatcgttt aatgaatcgt cagcggtggc ttgattactg
     5221 gcaaaatatt tattcgcgct cgcttagcgt tcttccctac tttctgttat tgccgcagtt
     5281 tattagcggg cagatcaatc tgggcggact gatgaaatcg cgccaggcat ttatgctagt
     5341 atcgaacaat ttaagctggt ttatttataa atatgacgaa cttgctgaac tggctgcggt
     5401 tatcgatcgc ttgtatgagt tccatcaact cactgaacag cgccctacga ataagcctaa
     5461 aaattgccaa catgcggtac aagtggctaa tgcgagtatt cgtacgcctg ataataagat
     5521 catattagag aacttgaact ttcatgtttc gccaggcaaa tggctattac tgaaaggcta
     5581 ctctggtgcg ggaaaaacca cactgcttaa aacattatcc cactgctggc cgtggtttaa
     5641 aggtgatatt tcttctcctg ctgacagttg gtatgtgtca caaacaccgt taatcaaaag
     5701 cggcttactg aaagagatta tttgtaaagc acttcccctg cccgtagacg ataaatcgtt
     5761 gagcgaagta ctgcatcagg ttggtctggg gaaattggct gcgcgtattc atgaccacga
     5821 tcgctgggga gatattcttt ccagcggcga aaaacaacgt atcgccctgg cacgattaat
     5881 tttacgacgt ccgaaatgga tatttcttga cgaaactacc tctcatcttg aggaacaaga
     5941 ggctatacgc ttactgcgtt tagtgcgtga aaaactaccc acaagcggcg tcattatggt
     6001 tacacatcaa cccggtgtct ggaacctggc tgatgatatt tgtgacatta gcacggtttt
     6061 ataacaacaa taaaagccct gctgtacgga gatataaata aatgaagcga gttcttattc
     6121 ctggcgtcat tttatgtggc gctgatgtgg cgcaggccgt cgatgacaaa aacatgtaca
     6181 tgcatttttt tgaagagatg acggtctatg ctcctgtccc tgtacccgta aacggcaaca
     6241 cgcattacac cagtgaaagc atcgagcgtt taccgaccgg gaatggcaat atcagcgatc
     6301 tgctgagaac caaccctgcg gtacgcatgg attcaacgca aagtacctcg ttgaaccagg
     6361 gagatattcg ccctgagaaa atctctattc acggtgcgtc gccctaccag aatgcctatt
     6421 tgattgacgg tattagtgca actaataacc tgaacccagc gaatgagtcc gatgccagta
     6481 gtgcaaccaa tattagcggg atgtcacagg ggtattatct tgatgtcagc ttactggaca
     6541 atgtgacgct ttatgacagt tttgtgccgg ttgaatttgg tcgcttcaat ggcggggtaa
     6601 ttgatgcaaa gatcaaacgc ttcaacgctg atgatagcaa ggtgaaattg ggttatcgca
     6661 ctacgcgttc ggactggtta acatcgcata tcgatgagaa taacaagagc gcatttaatc
     6721 aaggctcttc aggaagtact tattactccc cagattttaa aaagaacttt tataccttgt
     6781 cgtttaatca ggaactcgct gataacttcg gcgttaccgc cggtttatcg cgccgccagt
     6841 ctgatatcac ccgcgcggat tatgtttcga atgacggcat tgtcaccggt cgggcacagt
     6901 ataaaaacgt tatcgatact gcattgagca aatttacctg gtttgccagc gaccgcttta
     6961 cccacgattt aaccttaaaa tataccggct ccagccgtga ttataatacc agcaccttcc
     7021 cgcagtctga tcgcgaaatg ggtaataaat cctatggtct ggcatgggat atggatacgc
     7081 aactcgcatg ggccaaacta cgtaccaccg ttggttggga tcatattagt gattataccc
     7141 gtcacgatca tgacatctgg tacaccgaac tttcatgtac atatggtgat attacagggc
     7201 gttgtacctg tggcggatta ggacacattt cccaggctgt agataattac accttcaaaa
     7261 cacgcctgga ctggcaaaaa ttcgccgtgg gtgatgtttc gcatcaaccc tacttcggcg
     7321 cggaatacat ctattccgat gcatggactg aacgccataa ccagtctgaa tcctatgtga
     7381 ttaatgctgc aggaaagaaa actaaccata ccatttacca taaaggtaaa ggcagcctgg
     7441 gaattgacaa ctacacgctg tatatggtgg agcacattag ctggcggaat gtgtcgttaa
     7501 tgcccggtgt gcgttatgac tatgacaact atctgtcaaa ccacaatatc tccccgcgct
     7561 ttatgacgga atgggatatt tttgctgatc aaacctcaat gattaccgcc ggttataacc
     7621 gttactatgg cgggaatatt cttgatatgg gattacgtga tatccgcaat agctggacgg
     7681 aatcggtatc aggtaataaa actctgacgc gttatcagga tttgaaaacg ccttataacg
     7741 atgaactggc aatgggattg cagcagaaaa tcggtaagaa cgttattgcg cgtgcaaact
     7801 atgtttaccg tgaagcgcat gatcaaatca gcaaaagcag tcgtaccgac agcgcgacta
     7861 aaaccaccat tactgaatat aacaacgatg gcaaaaccaa aacgcattcg ttcagcctca
     7921 gttttgaact ggccgaaccc ctgcatatcc gccaagtaga tattaaccca caaattgtct
     7981 ttagctatat caagagcaag ggcaacttgt cgttaaacaa tggttatgag gagagcaata
     8041 ccggtgataa ccaggtggtt tataacggta atctggtctc ttacgatagt gttccagtgg
     8101 cagattttaa taacccatta aagatctcct taaacatgga tttcacgcat caaccgagcg
     8161 ggttagtgtg ggcgaatacg ctggcctggc aagaagcgcg taaagctcgc attatccttg
     8221 gtaagacaaa tgcgcaatac atcagcgaat attcagatta caagcagtat gttgacgaaa
     8281 aactggatag cagcctgacc tgggacaccc gcttgtcctg gacgccacaa tttctgaaac
     8341 aacaaaacct gacgatcagt gccgatattc tcaatgtact ggatagcaaa accgctgttg
     8401 atacaacgaa caccggtgtg gcgacctacg ccagtggccg tactttctgg cttgatgtca
     8461 gcatgaaatt ttaatagttc ggattttact taaccgggta aattcgcccg gttttcgcat
     8521 ggagataatt atgagaaacc tctgtttctt actgacgtta gtggcaactc tgttgctccc
     8581 cgggcggctg attgccgccg ccttaccgca ggatgaaaag ttaattaccg ggcaactgga
     8641 caatggcttg cgatatatga tttatccgca tgctcaacca aaggatcagg taaatttatg
     8701 gctgcaaatt cataccggtt cattgcagga agaagacaat gagcgcggcg tggctcattt
     8761 tgtagaacat atgatgttta acggcacaaa aacatggccg ggtaataaag tcatcgaaac
     8821 atttgagtca atgggcctgc gtttcggtcg cgatgttaat gcctatacca gctatgacga
     8881 aacgctgtat caggtgagtc tgccgactac gcagaaacaa aatctgcaac aagtgatggc
     8941 aatcttcagt gaatggagta atgccgcaac ctttgaaata ctcgaagtag acgctgaacg
     9001 cggcgtaatt actgaggaat ggcgtgccca tcaggatgcg aaatggcgca cctctcaggc
     9061 gcgccgccct ttcctgctgg caaatacccg taatttagac cgtgaaccta tcggcctgat
     9121 ggatactgtc gccacggtca caccggcaca attgcgccaa ttttatcaac gctggtatca
     9181 accaaataat atgaccttta tcgtggtcgg cgatatcgac agtaaagaag cgctggcgct
     9241 gataaaggat aatttaagta agcttccggc taacaaagca gctgaaaatc gcgtctggcc
     9301 gacaaaagcc gaaaaccacc tgcgctttaa tatcattaat gataaagaaa accgagtgaa
     9361 cggcatcgca ctctattatc gcctgccaat ggtacaagtg aacgatgagc aaagctttgt
     9421 cgaacaagct gaatggagca tgttagttca gctgttcaat caacgtctgc aggaacgcat
     9481 acagtcgggc gagttgaaga ctatttctgg cggcactgcg cgcagcgtta aaattgcacc
     9541 cgattatcag tcgctgtttt tccgtgtaaa tgcacgagac gataatatgc aggatgctgc
     9601 aaatgcatta atggcagagt tggcaaccat tgatcagcat ggtttttctg ctgaagaact
     9661 cgatgatgtc aaatctaccc gcctcacctg gctgaaaaat gcggttgatc agcaagctga
     9721 acgtgattta cgtatgctga ccagtcgcct ggcatccagc tcattaaata atacgccgtt
     9781 cttgtcgccg gaagagacat atcaactttc gaaacgtctg tggcagcaaa ttaccgtgca
     9841 aagtctggcg gaaaaatggc agcagttaag aaagaaccag gacgcatttt gggagcaaat
     9901 ggtaaacaat gagcttgccg ccaaaaaagc attgtctctt gcggctatcc tggcgctgga
     9961 aaaagagtac gccaacaaaa agctggcggc ttacatcttc ccaggcagaa atttatcgtt
    10021 aacagtagac gctgacccac aggcggaaat tagcagcaaa gaaacgctgg ctgagaatct
    10081 gacatcatta acactttcca atggtgccag ggttattctg gcaaaatccg cgggtgaaga
    10141 gcaaaagcta caaattactg ccgtatctaa taaaggcgat ttaagtttcc ctgcgcagca
    10201 aaaatcactt atcgcgctgg caaataaagc agtaagcgga agcggcgttg gtgagctctc
    10261 ctcttccagc ctgaaacgct ggagtgcgga aaattcggta accatgagca gtaaagtcag
    10321 tggcatgaat acgttgctct ccgttagcgc gcggactaat aaccctgaac ctggttttca
    10381 gttgattaac cagcgaatca cccacagcac gattaacgac aatatttggg catcgctaca
    10441 aaatgctcaa attcaggcgt tgaaaacgct cgaccagcgt ccagcggaga aattcgccca
    10501 gcagatgtat gagacgcgct atgctgatga ccgcacgaaa ttactgcaag aaaatcagat
    10561 tgtacagttt actgccgcag atgcgctggc tgccgatcgc cagttgtttt catctccagc
    10621 ggatatcacg tttgtcattg tcggtaatgt ctcagaagac aaactcgtgg cgttaattac
    10681 gcgttactta ggatcaatca aacactctga ttcgccatta gccgcaggta aaccattaac
    10741 tcgcgcgacg gacaacgcat cggttactgt aaaagaacaa aatgaacctg tggcacaggt
    10801 ttcacagtgg aagcgttatg attcccggac acctgttaat ctggcgacgc gtatggcgct
    10861 cgatgctttt aacgtcgcac tggcaaaaga tctacgcgtt aatattcgtg aacaggcatc
    10921 tggagcatac agcgtttctt ctcgcctctc ggttgatcct caggccaaag atatcagtca
    10981 tttgctggct tttacttgtc aaccagaacg acatgatgaa ctgttaacgt tagcgaatga
    11041 agtgatggtt aagcgtctgg ctaaagggat cagtgagcaa gaactgaatg aataccagca
    11101 aaacgttcag cgcagcctcg atatccaaca gcgtagcgtt caacaattag cgaacactat
    11161 tgtaaatagt cttattcaat atgacgatcc tgcagcatgg actgagcagg agcaattgtt
    11221 gaaacaaatg acggtagaga atgttaacac tgccgttaaa caatatcttt ctcatccggt
    11281 aaatacttat actggagtat tattgccaaa ataataacag ccccgtcaac acatcgttgg
    11341 cggggatttt agcaatattc gccattttta tgtaataatt ttataaatgc gttcaaaata
    11401 ataaccaggt actaatagtg atattttaag gtctgatttt tacgtgaaaa ttcaggaggc
    11461 acagaatgcg cataaaaata acagcataaa acaccttacc accacccaag aatttcatat
    11521 tatattgttt ttcaatgaaa aaatattatt ctcgtaatat ctcacgataa ataacattag
    11581 gattttgtta tttaaacacg agtcctttgc acttgcttac tttatcgata aatcctactt
    11641 ttttaatgcg atccaatcat tttaaggagt ttaaaatgga taagaagcaa gtaacggatt
    11701 taaggtcgga actactcgat tcacgttttg gtgcgaagtc tatttccact atcgcagaat
    11761 caaaacgttt tccgctgcac gaaatgcgcg acgatgtcgc attccagatt atcaatgacg
    11821 aattatatct tgatggcaac gctcgtcaga acctggccac tttctgccag acctgggacg
    11881 acgaaaatgt ccacaaattg atggatttat ccattaa
//
LOCUS       DAZPEP010000124        11820 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZPEP010000124 DAZPEP010000000
VERSION     DAZPEP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11820
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.124"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..185)
                     /locus_tag="ACHUT9_003220"
     CDS             complement(<1..185)
                     /locus_tag="ACHUT9_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1349627.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGA"
     gene            complement(230..1216)
                     /locus_tag="ACHUT9_003221"
     CDS             complement(230..1216)
                     /locus_tag="ACHUT9_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFR1349628.1"
                     /translation="MKMRSFTRSLVCASLLALVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSEGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQKQILETAAKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
     gene            complement(1527..2663)
                     /gene="hemW"
                     /locus_tag="ACHUT9_003222"
     CDS             complement(1527..2663)
                     /gene="hemW"
                     /locus_tag="ACHUT9_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFR1349629.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(2656..3249)
                     /locus_tag="ACHUT9_003223"
     CDS             complement(2656..3249)
                     /locus_tag="ACHUT9_003223"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFR1349630.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(3257..3547)
                     /gene="yggU"
                     /locus_tag="ACHUT9_003224"
     CDS             complement(3257..3547)
                     /gene="yggU"
                     /locus_tag="ACHUT9_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFR1349631.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(3544..4110)
                     /gene="yggT"
                     /locus_tag="ACHUT9_003225"
     CDS             complement(3544..4110)
                     /gene="yggT"
                     /locus_tag="ACHUT9_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFR1349632.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(4128..4832)
                     /gene="yggS"
                     /locus_tag="ACHUT9_003226"
     CDS             complement(4128..4832)
                     /gene="yggS"
                     /locus_tag="ACHUT9_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFR1349633.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            4850..5830
                     /locus_tag="ACHUT9_003227"
     CDS             4850..5830
                     /locus_tag="ACHUT9_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFR1349634.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            complement(6005..6421)
                     /gene="ruvX"
                     /locus_tag="ACHUT9_003228"
     CDS             complement(6005..6421)
                     /gene="ruvX"
                     /locus_tag="ACHUT9_003228"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFR1349635.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(6421..6984)
                     /locus_tag="ACHUT9_003229"
     CDS             complement(6421..6984)
                     /locus_tag="ACHUT9_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFR1349636.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(7093..8043)
                     /gene="gshB"
                     /locus_tag="ACHUT9_003230"
     CDS             complement(7093..8043)
                     /gene="gshB"
                     /locus_tag="ACHUT9_003230"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFR1349637.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(8056..8787)
                     /gene="rsmE"
                     /locus_tag="ACHUT9_003231"
     CDS             complement(8056..8787)
                     /gene="rsmE"
                     /locus_tag="ACHUT9_003231"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFR1349638.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(8867..9574)
                     /gene="endA"
                     /locus_tag="ACHUT9_003232"
     CDS             complement(8867..9574)
                     /gene="endA"
                     /locus_tag="ACHUT9_003232"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFR1349639.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(9669..10166)
                     /locus_tag="ACHUT9_003233"
     CDS             complement(9669..10166)
                     /locus_tag="ACHUT9_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFR1349640.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(10243..11637)
                     /gene="galP"
                     /locus_tag="ACHUT9_003234"
     CDS             complement(10243..11637)
                     /gene="galP"
                     /locus_tag="ACHUT9_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFR1349641.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         2876 a         3051 c         3016 g         2877 t
ORIGIN      
        1 gcacctaata accctaccca gatcatcaaa aatcgtgcca gctcatcggt taatgtactt
       61 ggctggttaa gtacgtagcg gctaaatact tgccagacta cgcatacaac cagagcaagc
      121 atgacggcga cagaaaacgc ggcaatgacg cggtctaccg caagtttcag gcggttaaat
      181 atcattgtaa gcatcttgga aaatcagggc cagagactgg cccggaatat tattgagcag
      241 cgttgtcgaa cttctctaac aaagcagctt gttttggatc ttttttgaag tcgtcaaaca
      301 gaggctgaac tgcggcacgg aatggtgctt tatccacttt tacaatttca ccccccatag
      361 ctttcgcctg agcacgtgtg tcagcgtcaa ttttttccca caatttttgt tgataagctt
      421 cggatttctt cgctgctgtt tcgaggattt gtttctgttc tggggttaat ttattccatg
      481 ttttggtgga gataaccaaa aagtcaggaa ttgaggcgtg ttcatcttca gaaaaaactt
      541 tggcaatttc aatatgtcga gtctgcaccc aggaaggcac gttattttct gcgccatcta
      601 ctacaccttg ctgcatcgca gtataaactt cgccaaatga aattggagtg ggtgaaccac
      661 ccatcagttc aatcattttg atggtggtcg gacttggctg aacacgaatt ttcagccctt
      721 taagatcttc tggcttagta atcggttttt tcgcatagaa actgcgtgta cccgctacat
      781 aagctgataa agcaaaaaag cctttgtctt ttgttgagtc cataatttct ttaccaactt
      841 caccaaaaac aactttgttg aagtgagcct gatctttaaa taagaacggc aaattataga
      901 tagcataaat attatcaaaa gactccagat cactggcgga acctttggtc atatccagtg
      961 cgccattttg caacaattcc atagtttctc gcgcattacc catctggctg ctgggataaa
     1021 tacggatcac catattacct tctgacagct gttttacctc tttcgccagc tcttcaaaac
     1081 tctgatgtac aacatgactt ctttccaggt tgtgtgctaa ctttaacgtc actttttctg
     1141 cggcgtttac gccagtgcta accagagcca gcaaggatgc gcagactaat gagcgggtaa
     1201 atgacctcat tttcataaaa aaactccctg ttgaaggatg gaaattggtt gatatctaat
     1261 tgtgcttatc agtgctttgt gcgacaaatt ggttaacctg tctgacaact tgtctatgtt
     1321 tatcacaact tgaaataaag tcagaaatta aatattaaat ttgtgcgcca ggttgtacat
     1381 gtttataatc aataaatgat gagtaaatat tcactagata tatctaataa atatcatcga
     1441 tgtttattca gtaagaaata tattttgccg aagttaatta acggggggaa aagaagtgat
     1501 taatgcgccc gcaggcgcat taatgattac tcagcgagga ataattcaag aagtgagttt
     1561 aagaataatt taccgtgctc ggttatctgc cagtaatccg cacattcggt gagataaccc
     1621 tgggcaatcg cctcgtctaa ctgtgggcga atcacatctt cgcaaagccc ggtatacgca
     1681 ataaactcca cgcgcggcgc ggcctccagc agacggaagc gattcataaa gaactcaaac
     1741 ggcttatctg cggcttcgac atcacgctgg ctttccagat accttccttg cataaaacca
     1801 cgaggatgac gcgttttagt ggtacgcaga atgcgcccat ccgggaaggt gactttgccg
     1861 tgcgcgccgc agccaatacc gatgtagtca ccaaatcgcc agtagttgag attgtgctgg
     1921 cactgataac cgggtttggc gtaagcggaa gtttcatatt gctgataacc cgctgcggtt
     1981 aataactgat gcccctgttc gaaaatatcc cacagcgcgt catcgtccgg cagcaccggt
     2041 ggtcgcgaac caaacagcgt attgggttcg atggtcagtt gataccagga aagatgcggc
     2101 ggattcagtt caatggcctg gcgtagatcg ccaagcgcct cttccagtga ttgatccggt
     2161 agtccatgca tcaaatcgag gttaaagcta cgcagcccca gcccgctcgc cagctttgcc
     2221 gcgcgtttcg cttcttgcgg gccatgaata cgcccaagtc gtttcagctt ttcttcgcta
     2281 aaactctgca caccaataga gatgcggttc acaccagcac gctgataatc gacaaagcga
     2341 tcggcttcta ccgttccagg gttcgcttcc atagtaattt ctgcatccgc tgccagcggc
     2401 aaacgcgcac gcacgccgtc cagcagcgtt tgcatcgccg ggccggaaag caggctcggc
     2461 gtaccaccgc caataaagat tgtctttact tcacggccct gagcgtaagc cacatcattg
     2521 tccagatcgc aaagcagatg ctgaacataa tcgtcgtgcg gcacttctcc tttcaacgcg
     2581 tgagagttga aatcgcagta cgggcatttc tgcacgcacc acgggatgtg aatgtagaga
     2641 ctcagcggcg gtaatttaac cattacgtaa agcgtccagc agcagtttca acgcctgacc
     2701 acggtgggaa atggcgctct tttcttcgcg ggtcagttcg gcggcggttt tcccttcgga
     2761 aggtacgaag aagattggat cataaccaaa gccaccagtg cccgctggtt cgcgagtaat
     2821 cacgcccggc cagctgccgt ggcataccag cggagtcgga tcttccgcgt gacgcagata
     2881 taccagcacg cagtggaaac gcgcctgacg ttggtcgtcc ggtacgtctt tcagtgtttc
     2941 caacagtttt tgcagattct tttgatcggt cgcgtcttca ccggaataac gcgcggagta
     3001 aatacccggc gcgccaccca gcgcatctac cgccagacca gagtcgtcgg caattgccgg
     3061 taaaccggtc acttttgccg catagcgcgc tttcagaatc gcgttttcga taaaggtcag
     3121 gccggtttct tcagcggaat ctacgccgag gtctgtttgg gccacgatat caagaccgaa
     3181 gtcgctaagc agcgacgcca gctcacgcac tttaccggca ttgccggttg cgaggacaac
     3241 tttttgcata ggatacctaa ttaattaacg ccgcgatttc tggcgggatt tgttgcggat
     3301 taatgatttt aatttgtttg tggcggccaa gttcgccttt ttcaatcacc acctggcttt
     3361 tggcaacccg gaattgctta ccgagaaact tcaccagatg actgttgccc tggccgtcaa
     3421 ccggcggcgc ggtaatggcg actttaactt cgtcgccatg taaaccgaca atagaatcac
     3481 ggctggcttt cggctgaata tagagccgta aaaccagacc gtcatcatta actgttacgg
     3541 cactcataac gccatccaca gccccggcag cagcatgttt ccggttgcct gtaatacttc
     3601 tgcgacaccc atattgatga catacagcag cagaacgagg atcatcggcg agaaatcaat
     3661 cccacccatt gccggtagca ggcggcgaat cgggcgcagc agcggatcgg ccagctgaat
     3721 cagcacgtat tcaatcggac tacgaccctg gcttacccag ctcataatcg ccatcaccag
     3781 caggacccag aaaatcagca ggccgatggt tttcagcaga atcagtaaac cagcaatcca
     3841 gatgattggc aggaaggtca ccactttaaa cagcacgatg gctttgataa aactgagaat
     3901 ataggcaacc agcagcgagg cgctgtcaat ggggcccatt gccggaataa cgcggcgcag
     3961 tggcccgata attggctgcg ttaccttcac tacaaactgt gagaaggggt tgtaaaaatc
     4021 acaatgagcc cactgcatcc agatgcgtaa taacagcacc atggtataca gctcaatgac
     4081 cgttgaaagc aggaaagtca acgtattcat ggcgttcctt taattcctta ttttttagag
     4141 taatcacgcg caccaaaaat tgcagtgccg atacgaacca tcgtgctacc tgccgcaatg
     4201 gcggcttcca tatcgtccga cattcccaga gagagcgtgt cgatatgcgg gtagcgcgtt
     4261 ttcagtccgg caaatgctac agccatttgg cgtgcaactt caaactgcct tacatattct
     4321 gactcaggcg cagggattgc catcagcccg cgcagacgta aacgcggtag ttcagcgacc
     4381 gccgctgcca gctcgtccag ttctgccagt tgaatcccgg acttactgtt ttcatcacta
     4441 atattaattt gaatcagtac gttaagaggg ggaagttctg ccgggcgctg atcgttgagg
     4501 cgggtagcaa tgcgcaaacg gtcgatggta tgacaccagt cgaaatgctc tgccaccagg
     4561 cggcttttat tagactgcaa cgggccaata aaatgccatt ctaatcctgt tacgcccagt
     4621 tcctgaaagt ggcgaatttt atctacccct tcctgaacgt agttttcacc aaattgacgc
     4681 tgcccggcat caatggcttc tgcgatggcg ctcgcaggtt ttgttttact gactgcaagc
     4741 agcgtaattt cttctggaga acggccgcaa cgcgttgcag ccgctgagat tttgtcccgg
     4801 acctgtgcca ggttatgcgc aatatcgttc attttccgag gatcttagta tgaatatgga
     4861 agaaattgtg gcccttagtg taaagcataa cgtctcggat ctacacctgt gcagcgcctg
     4921 gcccgcacga tggcgcattc gcgggctaat ggaagctgcg ccgtttgagg cgccggacgt
     4981 cgaagagcta ctgcgggagt ggctggatga cgatcagcgg gcaatattgc tggaaaatgg
     5041 ccagctggat tttgccgtgt cgctggcgga aaaccagcga ttgcgtggca gcgcatttgc
     5101 gcaacggcat ggcatttcgc tggcgttacg gctgttacct tcgcactgcc cgcagctcga
     5161 acagcttggc gcaccaacgg tattgccgga attactcaag agtgagaatg gcctgattct
     5221 ggtgacgggg gcgacgggga gcagcaaatc taccacgctg gcggcgatgg ttggctatct
     5281 caatcaacat gccgatgcgc atattctgac gctggaagat cctgttgaat atatctatgc
     5341 cagccagcga tgtttgatcc agaagcggga aattggtttg cactgtatga cgttcgcatc
     5401 gggattgcgg gccgcattgc gggaagatcc tgatgtgatt ttgctcggag agctgcgtga
     5461 cagcgagaca atccgtctgg cactgacggc ggcagaaacc gggcatttgg tgctggcaac
     5521 attacatacg cgtggtgccg cgcaggcagt tgagcgactg gtggattcat ttccggcgca
     5581 ggaaaaagat cccgtacgta atcaactggc aggtagttta cgggccgtgt tgtcacaaaa
     5641 gctggaagtg gataaacagg aaggacgcgt ggcgctgttt gaattattga ttaacactcc
     5701 cgcggtgggg aatttgattc gcgaagggaa aacccaccag ttaccgcatg ttattcaaac
     5761 cgggcagcag gtggggatga taacgtttca gcagagtttt cagcagcgag tgggggaagg
     5821 gcgtttgtga cgttgccatg cccgatgtgc aagcttatcg ggcctacaaa ggcaataaac
     5881 gtctgatgag acgtttgcgc gtcttatcag acctacaatc gctgccacat gtaggccgga
     5941 taggcgttca cgccgcatcc ggcaagatgg cggtaacgac tatccgccag gcttttaaat
     6001 cgccttaata tccctgctcg aaatagcttt cgagaataat aaccgcagag gcagagtcga
     6061 ctttgccttt gttgagcgcc cgatagccgc cctgttcaaa cagaccggaa cgggcttcca
     6121 cggtgctaag acgttcgtca tggagcttta cttcaacacc gaaacgacca tgaatacggt
     6181 tggcaaattt acgcgctctg gcagtcaatg gttgctcggt gccgtccata ttcagcggca
     6241 aaccgacgat gatttcgtcc ggctgccact ctttcagtaa acgttcgatg aggttccagt
     6301 ccggcgtacc gtcctgtgct ttaattgcag gcaaagggcg agcagtgccg gtaatgcgtt
     6361 ggccgaccgc tacgccaatg cttttggtgc cgaagtcgaa ggcgagtaag gttccactca
     6421 tcaggcgtgc cctgccacac caggcatggt gagaatatcc acaccaatca gttttgccgc
     6481 ctcgcgccag cggtcggcaa tcggcgtttt gaacagaata ttcagatccg ccggggccgt
     6541 tagccacgcg ttatcgagaa tttcttgttc cagttgacct ttttcccagg aggcataacc
     6601 tagagccacc aatacgtcag acggttgttt atcggtgccg agcgtttcca gcacatcgcg
     6661 ggaagtggtc atcactgtgt tgtctgaaat gcgaatgctg gaagcaaaat tggagggcgg
     6721 agtatgcaaa ataaacccgc gatcttcagc cagcggaccg ccgagcataa ccggtttatc
     6781 cagacggatt gattcatcac gcggctccgg cgtgatcttc agcttttcca gaatcccttc
     6841 aattttgaga ttttccagcg gcttgttgac gatgatcccc attgcaccat tggtattatg
     6901 ttcgcaaatg tagaccacgg aacgacggaa aatcggatcc tggagagcag gcatggcaat
     6961 aagaaagtga tgctgtaaat tcattgtcag aggttctgtt tcctggttca aaaaagcaac
     7021 agcgcccagt atgcgtggaa agaagggcgc tgtcactcag agtctcaacg agatccttct
     7081 cgctaaggtg ggttactgct gctgtaaacg tgcttcgatg gcatccatta acattccggt
     7141 gatcgacacc ggaaactctg cttcaatctc acgaatacag gttgggctgg tgacgttaat
     7201 ttcagtcaga cggtcgccga tgatatccag accaacaaaa atcagccctt tttctttcag
     7261 cgtcggcccg atctgacggg cgattttcca gtcactttcc gtcagcggac gaggttcacc
     7321 gcgaccaccg gcagccagat tgccacgggt ttcgcccccc tgcggaatac gcgccaggca
     7381 gtacggtacc ggctcgccat ccaccaccag cacgcgtttg tcgccatctt taatggctgg
     7441 caggtaattt tgtgccatgc agtagcgagt gccatgctca gtcagggttt cggcaatcac
     7501 gccgaggttt ggatcgcctt ctttcacgcg gaaaatcgac gcgccgccca taccgtccag
     7561 cggcttaaga atgatgtcgc tgtgtttctc ccagaacgct tttagctgcg ctttattgcg
     7621 cgtaaccagc gtttctggcg ttaagtcaga gaaccaggcg gtaaacagtt tctcgttaca
     7681 gtcgcgcagg ctctgcggct tgttaacgat cagcgtccct ttctcttcgg cacgttccag
     7741 aatataggtc gcgtagataa actcggtatc aaacggcggg tctttacgca tcaggatcac
     7801 atcgagatcg gccagcggca gatcctgttc accgacgaac gaaaaccact cttcgtagtt
     7861 ctgcttcacg ttcagcgtgc gggtatgggc gcgggcttcg ccattgatca gatacagatc
     7921 gcccatctcc atatagtgaa gttcgtaacc acgacgctgt gcttccagca acatagcgaa
     7981 actggaatct ttcttgatgt tgatgtttgc gatggggtcc atcacgatgc cgagcttgat
     8041 cattattctt ctccgttagc ccaaatcgcc aaatcgtact tgtagcgcgg taatggcggt
     8101 gagcgcagtt gtctctgtac gcaaaacgcg aggtcccaac aggatatcag taaattgata
     8161 gcgggcagtc atggcaattt catctgccga taaaccgcct tccgggccaa tcagcaggcg
     8221 gacgcgttca accggtaacg gcaacgtatt gatgctgtta ctggcgcgcg ggtgaagatt
     8281 cagtttcagt ccttcatcct gctctgcaca ccaggcttcc agatccatcg ctggacggat
     8341 ttccggcacc cggttacgac cacactgctc acaggcagca attgcaatct tctgccactg
     8401 ctggagcttc ttgttcagac gttcactatc cagtttaacg ccgcagcgct cagaaaaaag
     8461 tggcgtaatg aggcttacac cgagttcgat cgatttctgg atcgtaaatt ccattttttc
     8521 accacgcgac atcacctgac cgaggtgaat atgcagcgga gattcgcgat cgtcgatctt
     8581 gccttccagc accttcactt ccacgctttt tttgctggcg ctggtaattt cggcgtcaaa
     8641 gacctggttg ctaccgtcaa acaattgcaa cgcctgcccc ggccccatgc gcagtacgcg
     8701 cccgatatgg ttggcggcat cttcgcaaag cgcgatgtga gaatggctgg tcagtggttc
     8761 aggatgataa atgcggggga tacgcatagt taaaaatccg cgtcgtctcc ccacgcggtt
     8821 gtacgcgtgg ggtaggggtt aacaaaaaga atcccgctag tgtaggttag ctctttcgcg
     8881 cctggcaagc gcgttgcaca tacgggttat gattgccttg caccttcgcg atgcgttcat
     8941 cgcgctcgca ctcccagtcg gtaaccggat acatcttgtt ccatgcgttg aacagctgcg
     9001 tttgctggcg agagagtgtc aggttgtatt ggtcgcgcat atagaagtag gtgcgcgcaa
     9061 tggcaccgcg tgcacgcgct ggtggttcgg cagctttttc tttgaaatcg accttcatgg
     9121 cgcattgacc gtactggcct tcgccgccat tccactggct gtacataaag ttgccgcgat
     9181 cgccattcac ctcaccgact gacggctgca ggttatgcat atcgctttcc atcttgcgat
     9241 agaccggatc tttagcgcag tttttacgtc caccgtcctg ccagcactgg cgctggtgac
     9301 cgaactgcca ggcgggaacg acgtgttccc actctacgcg gctggctcgg ttttcatttt
     9361 tgcgcacctg atagccgcac gattgcagat caacaacgcc ttttttgccc tgccagttaa
     9421 ttttacatcc gcaataaaac gtaccgggcg cgtcagcgtg gacttttacc gccgcggctt
     9481 tcgcctgaga aaaactattg ataccttcgg ccaacgccgg gccggaaaat gctgcgctca
     9541 gtaccaccgc agcaatagac aaataacggt acatcactta ctccgtgtta aaacgagcca
     9601 gcaacgtagc gaaagttgtt ttggtatgca atcagccaga tcaggaaagt tcctgatagt
     9661 tcagatggtt atttcgcaac cagctgttca ccgcagtgaa cacagcgata gacggcctcg
     9721 ccacgaacta cgcgattatg gcggcgtacg gtaagctgat gctcctggca cttgcagcgg
     9781 taggggaagg tgttgcgacg cacggattgc agttcgaact gatgcgtacg acgggcggga
     9841 acacccagca cgctttccat catccacttc cactctttgc catgtggcgc tacgcggccg
     9901 aaatgtttcc ataccagcaa atgtgccagt tcgtgcggta ccacttcttc aataaaagct
     9961 tcactgtttt ccaacagcaa aacgggattg aggcggattt catagctttc cagccaggct
    10021 gttccggcgg aggttccgcg ctgggtgtaa gagagttttg gctccgggta gttacgccct
    10081 agcttcaggt tggcctgggc gagtttttcc cgcaggccgc gcataacggc ctgttggatg
    10141 gcgataggga gacgggatgt tttcatagcg gcagaggata gagcgaagaa aagatgactg
    10201 caagaggcgg cttcctccgc gacgggagga agcctgagga gattaatcgt gagcgccgat
    10261 ttcgcgcagt ttacgacctt tcatcaggtt acgttcaata tgttccagcg aaacgtgttt
    10321 ggtttccggt accagccaca atgtcagcag gataaacagt acgttcagag ccgcatacac
    10381 ccagaaggtg ttggcgttac ccagcgtgtt gagcatggtc aggaacgttg cgccaacgat
    10441 catgttggca atccagttgg tggcagtgga gcaggtgatg ccaaaatcgc ggcctttcag
    10501 cggctgaatt tcggagcaca gtacccaaat cagcggaccg gcactcatgg caaaaccgac
    10561 aataaacatc agcagcatgg cgatggcgaa atactgcgcc gacggagagt ggataccgat
    10621 atgcatcatt gtaccgagta cgcccatgcc agcagccatc accaggaagc ccagcgttag
    10681 cgttggtttt cgtccccagc ggtcaacaag gccgattgcg ataaaggtgg caagtacgtt
    10741 ggtcaggccg acaatcactg tcccccacat ttgctctgtg gtgttggtat aacccgccag
    10801 ttcgaagatt ttcggcgcgt aatacatgat gacgttcatc ccggtgaatt gctgcattac
    10861 ctgcaacagt acgccaagga acaccgcgcg gcggaagttg ctgttctctt taaacagcgc
    10921 ccagccactc tgtttaacct gcaaactttc acggatttca tccagttcgc gtttcgcttc
    10981 cgcgctggtg tcacgcaggc gtagcagcac gcgttcggca tcaacaaaac ggcgtttggc
    11041 ggcaaaccaa cgtgggctgt ctggcaggaa gaagacacca atcagcagca aaattgccgg
    11101 gatgataatc acacccagca tccagcgcca tgcaccggtg tagctgaagg cggtatcaga
    11161 aagataagca ccgaggatcc cgatagtgat catcaactga tacatcgaga tcatactgcc
    11221 acgaattttt tccggcgcaa tttcagagag gtacagcggt gcggtataag aggccacacc
    11281 caccgccagc cccagtagaa cgcgggaaag aatcagtact tcaacgtttg gcgcagccgc
    11341 agagaacagc gaaccggcaa caaacaaaat tgcgccgatc atcaggctct ttttgcgccc
    11401 gagtttaaag gagagccagc cgctgcccac cgcaccgact gccgcaccga acatcatgga
    11461 gcttacgacc cattcttgcg tgtgcgaagt aatctggaat tcatctgcaa taaacggcag
    11521 tgcgccagca attacaccga tatccaggcc aaagagtaat cccgccagag cggcaaggaa
    11581 gcagacgaaa aacgtcattg ccttgtttga ccgcccctgt tttttagcgt caggcatgat
    11641 gccctccaat atggttattt tttattgtga attaagatag gtgagtacga cgtaaaaaga
    11701 tgtgaagcaa atcacatcag tgtaatcgct tacactaaca tttatcttag ttatggttta
    11761 agtgcatgaa aataattgag ttatatcatc atagattaaa cgctgttatc tgcaattaag
//
LOCUS       DAZPEP010000125        11811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZPEP010000125 DAZPEP010000000
VERSION     DAZPEP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.125"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..488
                     /locus_tag="ACHUT9_003235"
     CDS             <1..488
                     /locus_tag="ACHUT9_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1349642.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="ACHUT9_003236"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="ACHUT9_003236"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFR1349643.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHUT9_003237"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="ACHUT9_003237"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFR1349644.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHUT9_003238"
     CDS             complement(3007..3180)
                     /gene="ynaL"
                     /locus_tag="ACHUT9_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFR1349645.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHUT9_003239"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="ACHUT9_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFR1349646.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHUT9_003240"
     CDS             complement(4452..4499)
                     /gene="ydaN"
                     /locus_tag="ACHUT9_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFR1349647.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="ACHUT9_003241"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="ACHUT9_003241"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFR1349648.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="ACHUT9_003242"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="ACHUT9_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFR1349649.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="ACHUT9_003243"
     CDS             complement(6839..7747)
                     /locus_tag="ACHUT9_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1349650.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="ACHUT9_003244"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="ACHUT9_003244"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFR1349651.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="ACHUT9_003245"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="ACHUT9_003245"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFR1349652.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11811
                     /locus_tag="ACHUT9_003246"
     CDS             10715..>11811
                     /locus_tag="ACHUT9_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1349653.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMG"
BASE COUNT         3005 a         3001 c         2930 g         2875 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattctc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctgcctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagctaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaag tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgcc
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacatgg g
//
LOCUS       DAZPEP010000126        11778 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZPEP010000126 DAZPEP010000000
VERSION     DAZPEP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11778
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.126"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..611)
                     /locus_tag="ACHUT9_003247"
     CDS             complement(<1..611)
                     /locus_tag="ACHUT9_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFR1349654.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUT9_003248"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="ACHUT9_003248"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1349655.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUT9_003249"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="ACHUT9_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFR1349656.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUT9_003250"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="ACHUT9_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFR1349657.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /locus_tag="ACHUT9_003251"
     CDS             complement(5085..6716)
                     /locus_tag="ACHUT9_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFR1349658.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUT9_003252"
     CDS             6897..7127
                     /gene="yjdI"
                     /locus_tag="ACHUT9_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFR1349659.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="ACHUT9_003253"
     CDS             7139..7411
                     /locus_tag="ACHUT9_003253"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1349660.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHUT9_003254"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="ACHUT9_003254"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1349661.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHUT9_003255"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="ACHUT9_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFR1349662.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHUT9_003256"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="ACHUT9_003256"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFR1349663.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHUT9_003257"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="ACHUT9_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFR1349664.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11778)
                     /locus_tag="ACHUT9_003258"
     CDS             complement(11524..>11778)
                     /locus_tag="ACHUT9_003258"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFR1349665.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3043 a         2857 c         2893 g         2985 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcaggg gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggcgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gcgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggt
//
LOCUS       DAZPEP010000127        11698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZPEP010000127 DAZPEP010000000
VERSION     DAZPEP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.127"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            197..301
                     /locus_tag="ACHUT9_003259"
     CDS             197..301
                     /locus_tag="ACHUT9_003259"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFR1349666.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(350..1603)
                     /locus_tag="ACHUT9_003260"
     CDS             complement(350..1603)
                     /locus_tag="ACHUT9_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1349667.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="ACHUT9_003261"
     CDS             1710..2603
                     /locus_tag="ACHUT9_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1349668.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="ACHUT9_003262"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="ACHUT9_003262"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFR1349669.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="ACHUT9_003263"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="ACHUT9_003263"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFR1349670.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHUT9_003264"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="ACHUT9_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFR1349671.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHUT9_003265"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="ACHUT9_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFR1349672.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHUT9_003266"
     CDS             complement(6839..7693)
                     /gene="ynfH"
                     /locus_tag="ACHUT9_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFR1349673.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHUT9_003267"
     CDS             complement(7695..8312)
                     /gene="ynfG"
                     /locus_tag="ACHUT9_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFR1349674.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHUT9_003268"
     CDS             complement(8323..10746)
                     /gene="ynfF"
                     /locus_tag="ACHUT9_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFR1349675.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVEYDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPAYTPDFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(10807..>11698)
                     /locus_tag="ACHUT9_003269"
     CDS             complement(10807..>11698)
                     /locus_tag="ACHUT9_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFR1349676.1"
                     /translation="ADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPI
                     YWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRK
                     DPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWELEKDEVISPLPVYAS
                     TFEGWDSPERSTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIAT
                     GDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLR
                     PSPLAKGNPQHTNLVEIEKI"
BASE COUNT         2961 a         2965 c         3018 g         2754 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacaaataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct agatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccacaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcggggtca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa atctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgggtc gcggtcattt ccgtaccatg
     9421 gtcgatggca tcagtccatg taaagacgga aatctgcgtt ttaacaggat tctcgagcat
     9481 cgggaaccat tctaccccca gatcccagct accttcgcgt acgcctgagt tgccgccgtt
     9541 tatgccgacg ttaccggtga gaacggaaag catggcaata gcgcgggatg tttgttcgcc
     9601 gttggagtgt cgttgtggcc cccaaccctg acaaatataa gcaggttttg ctgaaccgat
     9661 ctctcgtgcc aactggataa ttttttctgc cgggatgctg gtgatttttg ctgcccattc
     9721 cggcgtttta gctatgccgt caggcccttc gcccagaata taggctttat aatgcgcgtt
     9781 acgtggtgcg ttggcgggca gcgttttttc atcgtaacca acacaatatt tgtcgagaaa
     9841 tggctgatcg accatgtttt cagtaatcag tacccaggca atcgcacagg ccagtgcgcc
     9901 atcggtgcca gggcgaatgg gcagccattc atcttcacgc ccggcagctg tgtcgttata
     9961 acgtggatcg atgacgatca tgcgtgcgtt tgaacgttcg cgggcttgct cgacgtagta
    10021 agtgacacca ccaccgctca tccgcgtttc tgccgggtta tttccgaaca taacgaccag
    10081 tttcgtattg gcgatatcat ccgggctgtt gccatcattg gcaccgaaca tataactcat
    10141 tgcggcactg atctgtgcgg tactgtagct gccatagcga ctgagaaaac caccgcaaga
    10201 gttcatcaga cggtacggga cgtttgagtt ggtgatgttt ccgccatcta cacctgttcc
    10261 gtacaggaca tgtacagctt cattgccgta atcttttagg atccgccgaa gattatcact
    10321 gatggtatcc agggcttcgt cccaacttat ccgttcaaat ttaccttcac cgcgcttgcc
    10381 gacgcgcttc atgggatatt tcaacctatc aggatgattc atccgtcggc ggatagagcg
    10441 cccgcgtaaa cacgcgcgaa cctgatgatt accgtagacg tcgtcacctg tcgtatcata
    10501 ctccacccag tacacggtgt catctttcac atgcaaacgt aacagacagc ggctcccgca
    10561 gttaacggtg caggaactcc agaccgcttt ctcttctacc ggagcctctg ccgcccgaac
    10621 catttgggaa aatggcagag tgaaagcact gcttgccagc gcaaggctgc caagtgcgga
    10681 ggttttcatc agacttctac ggctgatttc agccttcatg agcgcctctg tggtatggat
    10741 tttcatcatt actcacttat tgcttttcaa acaaaatgtc atgccagaat ttatggttgt
    10801 agtgggttat attttttcga tctcgaccag attagtgtgc tgcgggtttc ccttcgccag
    10861 tggtgaaggg cgcagagtgg ttagcgtatt cacacagccg ccatggtcga ttttatcgcc
    10921 agacatattg gcctcgtgcc aggctccctg gcccatagcg ctaaccccag ggagaatgcg
    10981 tggtgttact ttggctggta gccgaacttc gccacgatgg ttaaacaccc gcaccatatc
    11041 gccggtggca atcccacgtt tctgtgcatc tatagggttg atccacactt cctgacggca
    11101 ggctgccttc aggacatcaa tgttgccgta ggtcgagtga gtacgggatt tgtaatggaa
    11161 accaaacagt tgcagtggga aggtactacg ttcaggggag tcccagcctt caaaggttga
    11221 ggcataaact ggcagtgggc ttatcacttc atctttttcc agttcccagg tacgggcaat
    11281 ttccgccagc ttgctggaat aaatttcaat cttaccggaa ggcgttttaa gtggatttgc
    11341 ctcggggtcg tcacgaaatg ctttgtaggc gacaaaatga ccattgggat ctttacgctt
    11401 atagataccc atttttttca gttcgtcgta agacggtaac gccggatctt tggcaagcat
    11461 tttggcgtac agatgttgta accattgttc ctgcgtgcga ccttcagtga acttttgata
    11521 gacgtcaggt ccaagacgtt tcgcgacttc actcaggatc cagtaaatcg gtttgcgttc
    11581 gaatttttcg ctggtgacag gctggaggaa aatgagatat cccatgttac cggcgtagtc
    11641 gttaggaata atatcttcct gctcaacggt catcaggtct ggcagcagaa tgtcggca
//
LOCUS       DAZPEP010000128        11680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZPEP010000128 DAZPEP010000000
VERSION     DAZPEP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.128"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..415
                     /locus_tag="ACHUT9_003270"
     CDS             <1..415
                     /locus_tag="ACHUT9_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1349677.1"
                     /translation="SMHGVKIKPWLPVKWTKELINDYLYGCLLSLYSICARDIYNMSA
                     GNNVKVAADAFLEIIFSLKNKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSC
                     FAASDQKAIQRYIPQSQDGEDFRSHLQYKLPLSAH"
     gene            626..949
                     /locus_tag="ACHUT9_003271"
     CDS             626..949
                     /locus_tag="ACHUT9_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFR1349678.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            946..1776
                     /gene="amiD"
                     /locus_tag="ACHUT9_003272"
     CDS             946..1776
                     /gene="amiD"
                     /locus_tag="ACHUT9_003272"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFR1349679.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1773..2786)
                     /locus_tag="ACHUT9_003273"
     CDS             complement(1773..2786)
                     /locus_tag="ACHUT9_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFR1349680.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2885..4315)
                     /locus_tag="ACHUT9_003274"
     CDS             complement(2885..4315)
                     /locus_tag="ACHUT9_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFR1349681.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4326..5327)
                     /gene="ltaE"
                     /locus_tag="ACHUT9_003275"
     CDS             complement(4326..5327)
                     /gene="ltaE"
                     /locus_tag="ACHUT9_003275"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFR1349682.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5364..7082)
                     /gene="poxB"
                     /locus_tag="ACHUT9_003276"
     CDS             complement(5364..7082)
                     /gene="poxB"
                     /locus_tag="ACHUT9_003276"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFR1349683.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7215..8183)
                     /gene="hcr"
                     /locus_tag="ACHUT9_003277"
     CDS             complement(7215..8183)
                     /gene="hcr"
                     /locus_tag="ACHUT9_003277"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFR1349684.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8195..9847)
                     /gene="hcp"
                     /locus_tag="ACHUT9_003278"
                     /gene_synonym="priS"
     CDS             complement(8195..9847)
                     /gene="hcp"
                     /locus_tag="ACHUT9_003278"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFR1349685.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9991..10890)
                     /gene="lysO"
                     /locus_tag="ACHUT9_003279"
     CDS             complement(9991..10890)
                     /gene="lysO"
                     /locus_tag="ACHUT9_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFR1349686.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11385..>11680)
                     /locus_tag="ACHUT9_003280"
     CDS             complement(11385..>11680)
                     /locus_tag="ACHUT9_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HFR1349687.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         2851 a         3080 c         3095 g         2654 t
ORIGIN      
        1 tagtatgcat ggtgtcaaga taaaaccatg gttgccagta aaatggacaa aagagttaat
       61 aaatgattat ttgtatggct gtttactatc tctgtactct atctgtgcaa gggatatata
      121 taatatgagc gccgggaata acgtgaaggt tgctgcggat gctttccttg aaattatttt
      181 ctctcttaag aataaatatt gcattaaact attaagcgct caggaacggg cgtttattta
      241 cgaatttgca agaatgatct tcgcttatat taatgacaaa agcattgaga tcttattact
      301 ttcttgtttt gcggcgtctg accaaaaagc aatccagagg tatatacctc agtcacaaga
      361 tggggaagac tttaggagtc atttacaata taagttgcct ctttccgctc attaatgttg
      421 ttggtgcaac ataataagac aggtaagcgg taccctataa tgaaaccaat atcgggttat
      481 gcaacggata atcgtaaaga cttaagtggg tgtttttgac acaatgagat gatttcctaa
      541 actgatcaaa accttcttcc caacatggca tatcagttcg ataatgcttt agagatcgaa
      601 ggcttaacaa acaaggagat cgatcatgca attttctaca accccaactc tggaaggcca
      661 gaccatcgtt gaatattgcg gtgtggtgac cggcgaagcg attttaggtg ccaatatttt
      721 ccgtgatttc tttgccggta tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa
      781 agaactgcgt aaagcacggg agatcgcctt tgaggaatta ggctcccagg cgcgggcgct
      841 gggggccgat gccgtcgtcg gtattgatat cgactacgaa acggtcgggc aaaacggcag
      901 tatgctgatg gttagcgtca gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc
      961 tggctggtcg ctgccgctct gttattggca gggtgtgcag gcgaaaaagg cattgtcgag
     1021 aaagagggat atcagcttga tacccgacgc caggcgcagg cggcgtatcc gcgcattaaa
     1081 gtgctggtga tccactacac cgcagatgat tttgatagct cgctggcgac actgaccgat
     1141 aagcaggtca gctcgcatta tctggtccct gcggtaccac cgcgatacaa cggtaaaccg
     1201 cgcatctggc aactggtgcc ggaacaagaa ctggcctggc atgcggggat tagcgcctgg
     1261 cgcggggcaa cgcgccttaa cgacacctct attggcattg agctggaaaa ccgtggctgg
     1321 caaaaatcgg ccggagtgaa atattttgcc ccgtttgaac cgacacagat tcaggcgctt
     1381 attccactgg cgaaagatat tattgcccgt tatcacatca agccggaaaa cgtagtggca
     1441 catgcggata tcgcaccgca gcgcaaagac gatccggggc cattatttcc ctggcagcaa
     1501 ctggcgcagc aggggattgg tgcctggccg gatgcgcagc gggttaactt ttaccttgcc
     1561 gggcgcgcgc cgcacacacc ggtagatacc gcgtcattgc tggagctttt ggcgcgctac
     1621 ggttatgacg ttaaacctga tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc
     1681 cagatgcatt tccgcccgac gttatataac ggcgaagcgg atgcagaaac tcaggcgatt
     1741 gccgaagcat tgctggagaa atacgggcag gattagcgcg gcagttttcc gtggtcgcgt
     1801 agccaggcgg cagttttctc gataccttca tctagggtga tgatcggctg ataacctaac
     1861 tcttcctgcg cacgcgtaat atccagcgta aagtcaaaat tcaacttgga gacgccgtag
     1921 tgggtcagcg gcggctcttt tgctgacttg cggcctaaac gctccatgct gcgggcgatc
     1981 atatccagca tcgggtaggg gacggaacga atacgacagt caatattcaa ctcgtcgatc
     2041 agcttctgca cgatgctgcg cagtgtgcga tgctcgccgt tggtgatatt gtacacacgc
     2101 ccggaaggta gcttatcgca ggcttcctgg cttgccagcc acattgcgtg cacggtattt
     2161 tcatagtagg tcatatcgac cagcgcactg ccgccatgcg gtaacagaat actgccgtag
     2221 tggtgcatca tatgcgccag acggggaata aagactttat cgtgcggtcc gaacagactt
     2281 tgtgggcgca gaatagtaaa gcgcgtttgt ggattcgcct gcgaaagcat attgatcact
     2341 tcttcgctgg ctgctttgct gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct
     2401 tctttaatat cgcgatggtg gtgataatcg aagtacaggg agggggaaga gatatgaata
     2461 aagttacgca caccccaggc gacagcccat tcacccaggc ggcgagtggc gcgaacgtta
     2521 gccagatcga aagcctgttg tgtcccccag ggtgaggtaa agctggagca gtgccacagc
     2581 gtatcaatgc ccgcgagcat cactttagct tgtgatgaaa ccagctcggt cagatccgcc
     2641 ggaacaaact ctgcgcccat tttttccagc aatttgccca ttgcctcgtt gcgaccggtc
     2701 gctcgcacgc tgatgccttt ctggcataaa aactctaccg cgtttcgacc taagccgctg
     2761 gtggcgccgg taaccagtac cttcatatca atccactgtt gttgagaaaa tgacgtgcgc
     2821 attcttccgt gatttccccc atgatgcaat gggaaacatg aaagaataac gcaggttttg
     2881 tcgattaatc tgtgctttgt tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa
     2941 taaacagatg cgcggggatc atcaataacc agtaaaacag ccccggcata ccgtgcggat
     3001 gccagaaagc gcggacatcg atagtacgat agtcgccttt atcttccagg ctaaaacaca
     3061 gtcgtcccag ccccgacgct ttcatgccaa ataacaacgt aagttgtttt tccggttcaa
     3121 cgacaatcac tttccagcta tccaccgcat cgccagtctg taaatattcg cgctccgggc
     3181 ggcctttcgc cagcttatga ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa
     3241 tattgccaaa gaaataacgc tctttaccgc cgatttggtt cactacctgc cataaagcag
     3301 caaggctggc ggacgtttta acggtaaacc ccgcctgttt ggcaaaataa ccgtactccg
     3361 gtcgccagcg ggcaaaggcc tgagcgtcgt agccccagtc gctggagttg accagttttt
     3421 cctcctcttt caacgtgcta cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga
     3481 gtgcacgtag cgcggtatca tccgccagca gatcgtgttt cagcccctga atcaacgccc
     3541 tggcggtggt gggcggtacg gaagtaatca cattgagaaa ccacaccgaa atccagcggg
     3601 tggggagggg gatggggatc aaccagcggc gcttaccgct caccgccata aaatgttcaa
     3661 actgttgctg ataactgagc acctctggtc cggcggcttc gaagatgcgg tgttcgctgg
     3721 ccggatgatc taacaacgcc accagatagt gcagcaagtt ttccagcgcg atgggcgtgg
     3781 tgcgtgaacg tacccagcgt ggcggcgtta acactggcag gttgtagacc atatcgcgca
     3841 tgacttcgaa cgccgctgaa cctgcgccaa cgataattcc ggcccgaagt tcggtcacag
     3901 gtacattcgc ttcacgaaga atgtccgccg tagcctgacg agcacgcaga tgatccgact
     3961 gctcatgtgg cggggcctgc aacgaactga gaaagattaa ttgcttaact ggtacttcac
     4021 gtagcgcatc gcggacgttg agagccacct ggcgctcctg agcgataaaa tcgccgcctt
     4081 cgcccatgct gtgcaccaga aaatagacgg tatcgatatc ctgcaacagg gccggaaggt
     4141 tatccggcca gctgagatcg actttatggc aactgacgtt tgccagttgc agctttgcaa
     4201 gcctgtcgac atgacgtgcc gccgccagga tctgatgccc ttgctggctg agtgtgcgca
     4261 ccagatgctg accaatgtag ccactggcac cgagaactaa aatgcgttgc ggcacgtctc
     4321 tctccttaac gcgccaggaa tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc
     4381 gagacgtcaa gatgcgtcac caggcggaca atcggcgagg cgttaatcag cacgtttctc
     4441 gctttcatgt attcgcctaa cgcggcagca ttttcttccc cgacgcgaac aaacagcata
     4501 ttagtgtcct gacgcatcac atccgcgcct gcttcacgca gttgctccgc catccaggcg
     4561 gcgttgtcgt gatcttcctg caaccgtgcg acgttatttt tcagggcata catcccggcg
     4621 gcagccagaa tcccggactg acgcatcccg ccaccggcca ttttccgcca gcggatggca
     4681 cgtttaatgt aatcacgatt accgacgagt aatgaaccga ctggcgtccc aagacctttc
     4741 gacaggcaaa tggtgaacga atcacaatat tgcgtgatct ctttcagttc gcagccgtaa
     4801 gccaccacgg cattaaagat gcgcgcaccg tcaacatgca gcgccagatt gcgcttgcgg
     4861 gtaaattccc atgcttcttt caggtattca cgcggcagca ctttgccgtt gtgggtgttt
     4921 tctagactga gtaatttggt gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc
     4981 actttatcca gcggtagcgt gccgtcggca gccgcgtcga tgggttgcgg ttgaatactg
     5041 cccagcaccg ctgcgccacc ggcttcaaac agataattat gcgcggcctg accgacaata
     5101 tactcttcgc cgcgttcgca gtgactgagc agagcgacca ggttagcctg ggtgccggtc
     5161 ggcagaaaaa tggcggcttc tttaccggaa agctctgcgg cgtagtcctg cagagcatta
     5221 acggtagggt cgtctccgta aacgtcgtcc ccaaccgggg cggccatcat tgcttcgagc
     5281 atggcgcggc tcggtcgggt aacggtatca ctgcgtaaat caatcatggc atgtccttat
     5341 tatgacggga aatgccaccc tttttacctt agccagttcg ttttcgccag ttcgatcact
     5401 tcatcgccgc gtccgctgat gattgcgcgc aacatataca agctaaatcc tttggcctgt
     5461 tcgagtttga tctgcggtgg aatggctaac tcttctttgg cgaccaccac atccaccagc
     5521 accggaccgt cgatggagaa ggcgcgttgc agggcttcat caacttcaga cgctttttct
     5581 acacggatac ccgtaatgcc gcacgcttcg gcaatgcggg caaagtttgt gtcgtgtagt
     5641 tcggtgccgt cagtcaaata gccaccagct ttcatctcca tcgccacaaa gcccagcacg
     5701 ctgttgttaa agacgacaat tttcactggc agtttcatct gcactactga gaggaaatcg
     5761 cccatcaaca tgctaaaacc gccatcgccg cacatggcga ccacctgacg ttctggctct
     5821 gtcgcctgcg cacccagcgc ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa
     5881 cctaacaggc gacgcttgcc gttcattttt agataacgtg ccgcccacac cgttggcgta
     5941 ccaacgtcac aggtgaaaat agcgtcatcg gcggcaaaat gactaatttg ctgcgccaga
     6001 tattgcgggt gaatggcttt ctcgctcggt ttagctaaat cgtccagccc tttgcgggcg
     6061 tcgcggtaat cttccagcgc tttatccaga aacttgcgat cggctttttc ttccaccaat
     6121 ggaagcaatg cacgcagagt cgacttgata tcgccgacca gtgccatatc caccttgctg
     6181 tgagcgccga tgctggctgg gttgatatca atctgaatga ttttggcatc ggtcgggtag
     6241 aaggcgcggt agggaaattg cgtgccgagt agcactaacg tgtcggcgtt catcatggta
     6301 tggaaacctg acgagaagcc gattaacccg gtcattccaa catcatacgg attatcgtat
     6361 tcgacatgtt ctttaccgcg cagggcatga acaataggcg ctttaatttt cccggcaaac
     6421 tcaactaact ctttatgcgc ccccgcgcag ccgctgccac acatcagggc gatattgctg
     6481 gaataacgca gcagttgcgc cagtttgcgt aactcttctt cttccggcgt cacgactggt
     6541 tgtggcgcat gataccagtg cgtggttgcc ccttctggcg caggttttaa cgccacgtcg
     6601 cctggtaaca cgacaaccga aacgccacgg ttaagcaccg ctttgcgcat ggcaatcgcc
     6661 agtacttgtg ggatctgctc cgggctggaa accagctcgc aatagtgact acattcgcgg
     6721 aatagctctt gtgggtgggt ttcctggaaa tagccgctgc caatttcgct ggagggaata
     6781 tgagcggcaa tcgccagtac cggaacgtga ttgcggtggc aatcgaacag gccgttgatt
     6841 aagtgcaggt tgccggggcc gcacgatccg gcgcagaccg ccagttctcc gctaagttgt
     6901 gcttcagcgc cagcggcaaa ggccgccact tcttcgtggc gggtggacat ccactcgatg
     6961 gtgcccatgc gattaagact gtcactaaga ccgttcagag agtcgcctgt gactccccag
     7021 atgcgtttca cccctgccga ttcgagtgtt ttggcgatat aagctgcaac cgtttgtttc
     7081 atggttctcc atctcctgaa tgtgataacg gtaacaagtt tagttcatct gacggagggg
     7141 gaagggatgg gagagaaagg aggcactaac ggttaaatag cccgatgaaa ggaatatcat
     7201 cgggcataag gcgattatgc gagaaccaaa tccccctgcg gatggcagga gcaggccagt
     7261 acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg tactgctcac cgtatattca
     7321 ccggaaacca cttttgtctt acagcagccg caaacacccg cacggcaggc agcgacaacc
     7381 ggaacgttat tgctttccag cgcctccagt agcgtggtgc caaccggggc gtaaaattct
     7441 cgtgccggtt gcagtttggt gaatttcaga ccgctggtcg ccgcttctgc tactggggtg
     7501 aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt tcacttcctg ctctacccaa
     7561 tccatatacg gagccgggcc gcaggtcatc acggtacgtg aagctaagtc aggtacacct
     7621 gccagcagtt cgcgagtgag acgaccagcg ataaagcctt cggtaacgtt attttctgcc
     7681 accagcgtta ccggatagtt acgccactca tcggcgaaaa taacatcctg cggcgtacgc
     7741 acgttgtaga tcacccgcac atcggcctgt ggacggttct tcgcaagcca gcgacgcatc
     7801 gacataatcg gcgtgacgcc gcagcctgcc gccagcaaca ggaatttatc ttctgcttta
     7861 tcgtcgcagg taaattcccc catcgcgtcc gaaagccaga gataatcacc gcgttttaca
     7921 tcgcgcgtca gccactggga gccgacaccg tcatcaatcc gccgcacggt cagggtgata
     7981 tattcactca cgcctggcgt ggaggaaatg gtgtaagcac gcagcgtttc cgctgagttt
     8041 cgcacgctga ccagtgcata ttgcccggcg cgatatgggt agtaatcgtg gcaaatcagg
     8101 gaaatcgtcc acacatccgg cgtttcttgc gtaatgtgat gaacctgcat ccgccacggg
     8161 cattgattcg ttggcatcgt catcgacaaa ctccttacgc gctcaacagt tgcttcatgt
     8221 cttcttcaac agtggtgata gaacgcaggc cgaatttctc gttcagcacc gccagcaggt
     8281 ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat atttttcacg cccagagaaa
     8341 gcagcgtcag cagaatgacg atcgctttct gttcaaacca ggagagcacc agcgacagcg
     8401 gcagatcgtt cacaccacag cccagtttct ctgccagagt gacagccaga ataatcgctg
     8461 agtaagcatc gttacattga cctgcatcta ccagacgcgg cagaccttcg atatcgccaa
     8521 actcaagttt gttaaagcga tatttaccac aggcgagggt caggatcagg cagtcatccg
     8581 gcacgctggt ggcgaaatcg gtgaagtagt ggcgctcgcc gcgtgcgccg tcacagccac
     8641 caagcaggaa gatatgacgc agtttttcac ggctcaccag atcaatcagc gtatcagcag
     8701 cgccaagcag cgtctggcga ccaaaaccca cggtgataag gtgcggaatt tcgctgtacg
     8761 ggaagcctgc catctgttgc gcctgggtga taaccgcaga gaaatcatca ccgtccagat
     8821 gacgcacgcc aggccagcca acaatgctgc gggtccagat acgatcgtca taagcgccta
     8881 cggttgggtc gatgatgcag ttcgaggtca tcacgatggg gccagggaaa cgagcgaact
     8941 ccacttgctg attctgccag ccgctgccgt agttaccgac cagatgcttg aatttacgca
     9001 gctccgggta gccatgcgca ggcagcattt cgccgtgggt gtagacatta acgcccgtgc
     9061 cttcggtctg ttccagcagg ttgtagagat ctttgagatc gtgaccggaa atcagaatgc
     9121 atttacccgc cgtcgctttg acgttgacct gggttggcgt cgggtgaccg tatttaccgg
     9181 tttcgcctgc atccagaatg ctcatcactt tgaagttcat ctggccgatt tccattgaac
     9241 actcaagaag cgcgttcata tcggcaggcc aggtccccag ccacgccatg attttatggt
     9301 actgggcata aatatcgttg tcgtattgac cgagaacatg cgcgtgttcc atataggccg
     9361 ccgcaccttt caggccatac aggcacagca gacgcaggcc gagaatgttt tcgccaatcg
     9421 ccgctttatc tttgttaggg gtaaattctg ctgcctgacg ttgcagctcg ccgagatcat
     9481 cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg cgcgttggca tctacagcca
     9541 ggcattgtgc tttcagcgcc tcgcgcaagg caatcgcttc acgagcgtag ccgacaatac
     9601 gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa ggcacgtggc gcgaagctgt
     9661 caacatcgtg gttgatgatg ccgtattcac acgcttttac cgcccaggca gaaagccctt
     9721 gcagcgccgc gatgagtaaa tcctgaaggt cagaagtttc cgccgtttta ccacacatcc
     9781 cctgcgcgta tgagcagccg tttcctgccg gagtacggat agtttgttca cattgcacac
     9841 aaaacatgat cacacctttt aaagttatat ttaatataca tgtttaaggt taagacgctt
     9901 aacgcgggga taaaagggat ttttcatgca actttaaggg agattgattt agcgcaattt
     9961 tggcggcagg gatctaccgc cagagaggta ttacgcagag aaaaaggcga tgaggatcgg
    10021 cactaacagg ctaagaataa aaccgtgaac aattgccgcc gggaccatat ccagcccgcc
    10081 agtacgttga agaacgggca gggtgaaatc cattgatgtg gcaccgcata agcccagtgc
    10141 agtagagcgg ctgcggcgaa tcagcccagg gatcaacata atagcaatca gttcacgggc
    10201 cagatcatta aaaaacgccg cgctcccgat taccggacca aaagattcgg tcaataaaat
    10261 accggaaaga gaataccagc cgaaaccgga ggccattgcc agcgcggtat tgatggggag
    10321 atcaagaata aaggcgttaa ttaaaccacc aattaatgaa ctgacaacca ccaccacggc
    10381 gacaatcatt ccccggcgat taaggacaat ctgctttaag gtcatgccat tattgcgcaa
    10441 ctgaatacca acgaggaaaa gtagcaaaat taacgtgtat tcactggctt cggtcgcgtg
    10501 ttgtaagaaa gccagtccac tcagaccaat ggcaaaacca atcactacta cgccgcacag
    10561 ttttagcgac tccagcgcca tcgcaatacg cgacgggagt ttttcttgct gatggtggtt
    10621 gcgccacggc aggcctcgct ccagccacat cagggcggca atattacaca gtaaaataac
    10681 ggtaatactg acggcagaat aatgcagaat cgccaacagg ttactggcga ggttatcgag
    10741 aaacgccaga ctgataccca taaaaaagag aataaggtaa accatccagc ttaatagctg
    10801 attaataact tttaacgcag cttgttggcg aagcggaatg aggtaaccta caatcagggg
    10861 aaccagaatg attaacagcc cagaaaacat gaaaacccag tccttgcaaa gatgaagtcg
    10921 aaatgcgcga tgacacacta ctgaaagcgg aaggacgagt aaagttgcaa ttaaaaggaa
    10981 atgttatgca taaggagcag tagagtattc gttttcattt aaagatattc ttgcgcttta
    11041 attacaaact gcaccgatgt tggtggcgtc aaaatcgccg aggcgttccc tgaaggccgg
    11101 ggcagcccac atggatgtgg gctgagggcg cgttttacag ggatgttacc tcgcgcccga
    11161 cccggtagcc gtaagggata agtcgagggc accgcgcagc ggcgattttg ttcgccagag
    11221 cccggggggg cagggggcgg cggcgattgg ccgccccctg cgcgctcctt gcgccagtgg
    11281 caatatgtgg cttagctcat gaaaggagcg caacaagatg atgaatcaac atataacaac
    11341 atcttaaaaa aaggcctgac attacgccag gccttctgcg ttaattaatc acgcttttcc
    11401 agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac cacccagaag
    11461 aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt gctgcgcgcc
    11521 gggttaacag aagtgttagt caccggaata ctaattaagt gaatcagggt taaggccaga
    11581 ccaatagcga tcggcgcaaa acctgccggc gcgaatttgt cggttgcgcc gtggatcacc
    11641 aacaggaaac ctgcactcaa taccagttca actaccagcg
//
LOCUS       DAZPEP010000129        11538 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZPEP010000129 DAZPEP010000000
VERSION     DAZPEP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.129"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            49..678
                     /locus_tag="ACHUT9_003281"
                     /pseudo
     CDS             49..678
                     /locus_tag="ACHUT9_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895405.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            675..1289
                     /gene="prfH"
                     /locus_tag="ACHUT9_003282"
     CDS             675..1289
                     /gene="prfH"
                     /locus_tag="ACHUT9_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1349688.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1346..2803)
                     /gene="pepD"
                     /locus_tag="ACHUT9_003283"
     CDS             complement(1346..2803)
                     /gene="pepD"
                     /locus_tag="ACHUT9_003283"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1349689.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3064..3522
                     /gene="gpt"
                     /locus_tag="ACHUT9_003284"
     CDS             3064..3522
                     /gene="gpt"
                     /locus_tag="ACHUT9_003284"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1349690.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3614..4858
                     /gene="frsA"
                     /locus_tag="ACHUT9_003285"
     CDS             3614..4858
                     /gene="frsA"
                     /locus_tag="ACHUT9_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1349691.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4916..5317
                     /gene="crl"
                     /locus_tag="ACHUT9_003286"
     CDS             4916..5317
                     /gene="crl"
                     /locus_tag="ACHUT9_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1349692.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5356..6411)
                     /gene="phoE"
                     /locus_tag="ACHUT9_003287"
     CDS             complement(5356..6411)
                     /gene="phoE"
                     /locus_tag="ACHUT9_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1349693.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6699..7802
                     /gene="proB"
                     /locus_tag="ACHUT9_003288"
     CDS             6699..7802
                     /gene="proB"
                     /locus_tag="ACHUT9_003288"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1349694.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7814..9067
                     /gene="proA"
                     /locus_tag="ACHUT9_003289"
     CDS             7814..9067
                     /gene="proA"
                     /locus_tag="ACHUT9_003289"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1349695.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9182..9257
                     /locus_tag="ACHUT9_003290"
     tRNA            9182..9257
                     /locus_tag="ACHUT9_003290"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9215..9217,aa:Thr,seq:cgt)
     gene            9863..10021
                     /locus_tag="ACHUT9_003291"
     CDS             9863..10021
                     /locus_tag="ACHUT9_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349696.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2991 a         2684 c         2913 g         2950 t
ORIGIN      
        1 gtaatcatgc cgggtctggc tggctaacac cccattataa ggggtacgaa taaagtaacg
       61 gggcagtcta ttttacagcg gcatattgcc tcgttttcgc atcatggttt gcctgaaggc
      121 agtgacgacg cgctacggta tattgcggaa catgacgatg cgctggcgtt tgcgcgtatt
      181 aatcgccagc tgatctcttt acgcataatg caacaggtta aggccaccgg aagtccggtt
      241 ctggatgtgg cgcataactt tgttagcgcg tgtcaaatcg gcgatcaaca gggctggttg
      301 catcgtaaag gtgccacgcc ggatgacaac ggtctggtga ttattcccgg ttcactcggt
      361 gattactcct agcttgtaca gcctgttgcg aacgaaaaaa cgctccactc gctggcgcat
      421 ggggctgggc gtaaatgggg gcgcaccgag tgtaaagggc gtctggcagc gaaatacaca
      481 gcgacgcagc tctcacgtac tgaacttggc agtcgggtaa tttgtcgcga taaacaactc
      541 atctttgaag aagcgccaca agcctataaa tcggctgaaa gcgtggtgca atgtctggtg
      601 caggctggtt taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct caaaaacagt
      661 ggagggaaaa aaggatgatc ttgctacaac tctcctcagc gcaggggccg gaagaatgtt
      721 gtctcgcagt gaaaaaggcg ctggagcgat taattaaaga agctgcccga caagacgtcg
      781 cggtaacggt gttggaaaca gaaacgggcc gctactctga cacgctgcgt tcagctctgg
      841 tttctctgga tggcgataac gcatgggcat taagcgaaag ctggtgcggc actattcagt
      901 ggatttgtcc gagtccgtat cggcctcatc atgggcgcaa aaactggttt ctgggcattg
      961 ggcgttttac cgctgatgag caggaacaat cggatgcaat ccgttatgag acgttgcgtt
     1021 cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc gccacgcatt
     1081 tggcatccgg tattagcgtg aaggttcagt cagagcgtag tcagcatgct aacaagcggc
     1141 tggcacgatt gctgattgcc tggaagctgg agcagcagca acaggaaaat agcgcggtgc
     1201 tgaaatcgca gcggcgaatg ttccatcacc agattgaacg tggcaacccg agacggacat
     1261 ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa ggcgattaaa
     1321 cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc agttcagtca
     1381 gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga gagtgtggac
     1441 cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt ttgaacagac
     1501 cacattccag gcccgcgtgg ataatctgga tgttcggcgt tttgttgaac agacgctgat
     1561 aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag ccaggatatg
     1621 cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc atgctcacca
     1681 cgtagtcttt accgctgtcg atcagagaac ggatcagaca gtgaatttct acgttattat
     1741 cagtcatggt caccacaccg acgttcaggg aggtttcgac cacacctttt gccacgtctg
     1801 agttgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta tcgcgagatt
     1861 tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc agatttttct
     1921 ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga gatttcagga
     1981 cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc ttcacgcggg atggcgttac
     2041 gcagtgtgcc gccgttgaaa tcgataaggc gcagatccag ttcttctgca tgacccgcta
     2101 ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttcc ccgccggagt
     2161 gacccccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct ggaaccgctt
     2221 cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc atgtagattt
     2281 caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag ttgctctgta
     2341 agccgaacgc accgtccata ccggcttctt cggtcatggt cagcagcact tccagcgggc
     2401 cgtgaaccac gttttcgtca gccagaaccg ccagcgcaga cgccatacca atgccgttat
     2461 ctgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa ggctggatag
     2521 gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata tcgaggtggg
     2581 cctgtaagac gaccgattta cgattttcca tacctgcggt agcaggttta cgaatcagga
     2641 tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa ccaacaatgt
     2701 attcagcgag ttgctcttca tgataggacg ggtgaggaat agaacagatt ttggcaaaaa
     2761 tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt ctccttgtcg
     2821 atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt caacacaaga
     2881 caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg
     2941 taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc
     3001 gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt
     3061 cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc
     3121 gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt
     3181 ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt
     3241 atttccagct acgatcacga caaccagcgc gagcttaaag tgctaaaacg cgcagaaggc
     3301 gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg
     3361 attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt
     3421 ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg
     3481 gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca
     3541 ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct
     3601 ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa
     3661 acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca
     3721 gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg
     3781 gatctggcgc ggcattgacc cacgtgaaat cctcgacgtc caggcgcgta ttgtgatgag
     3841 cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc gtggtggcaa
     3901 ctggatttat gagtgggcca tccaggcgat ggtgtggcaa caaaaagcct gtgcggaaga
     3961 agatccgcaa ctcagtggtc gtcactggct gcatgcggct acgttgtaca acattgctgc
     4021 ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttgt caaaccgtgc
     4081 ctatgaagaa gccgcccagc gtctgccggg cacgatgcgg cagatggagt ttaccgtacc
     4141 cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc cgttcccgac
     4201 agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc tgtatgaacg
     4261 ttattttgcg ccgcgcggca ttgcgatgct gactattgat atgccgtcgg tgggcttttc
     4321 atcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgttt taaaggctct
     4381 gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc gtttcggcgc
     4441 taacgttgcc gtgcgtctgg catatcttga atcgccgcgt ctgaaagcgg ttgcctgtct
     4501 tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg tgccggaaat
     4561 gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg aagcgttacg
     4621 cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctggggcgtc gctgcccaac
     4681 gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg actcacgctt
     4741 aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc cggtgtatcg
     4801 gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct tgtgttaaaa
     4861 atttgctaaa atttgccaat ttggtaaaac agttgcatca caacaggaga ttgcaatgac
     4921 gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac taggcccgta
     4981 tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg ctgtatgcgt
     5041 caacgtgaaa ccggcaccgg aagtgcgtga gttctggggc tggtggatgg agcttgaagc
     5101 gcaggaatcc cgttttacct acagttacca gtttggtctg ttcgataaag caggcgactg
     5161 gaagagtgtt ccggtaaaag acactgaagt ggttgaacga ctggagcaca ccctacgtga
     5221 gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg aaccggcgga
     5281 tgattttcgt gatgaaccgg tgaagttaac ggcgtgagtg aaatgtgccg gatgtatcac
     5341 atccggcaat atccattaaa actgataggt catgccaacc gcgacaatat catcattatt
     5401 aatattcaat ttgttatcgc tatccagttg gttgatttta taatcaacaa acgctgacat
     5461 atttttgttg aaataatacg tagcaccgac gtcgatataa ttgaccagat cttcatcacc
     5521 gataccttca atatctttcc ctttcgataa gacataaccc agcgatggac gcagaccaaa
     5581 gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa agccgccagt
     5641 tattggcgtc attttgcgtg tttcagaata gaaagttgcc agataaatat tattggcatc
     5701 gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc tttgcaggtt
     5761 ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat cgctgccgcc
     5821 aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa cgtcgcggtt
     5881 ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc cgaagaagtc
     5941 ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt cggtctgcgc
     6001 cgaggaatcg ccaccaaatt ccgggaacat atcggtccag gcttccacgt catacaacgc
     6061 gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc cggcaaaagc
     6121 gagacgcgtt ttctgctgtg cagtatcact ctccgcttta tttccggcaa actccgcttc
     6181 ccaccgacca taaccagtca gttgatcgtt aatttgtgtt tcacctttaa aaccaaaacg
     6241 gatataactc tggtcgccat ctttactggc gttatcactc atataatgca tggctttaac
     6301 tttgccatag acatccagtt tattaccgtc tttattatat atttctgcag cctgtacaga
     6361 tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca ttttcattcc
     6421 tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca ggaaagtttt
     6481 taacctgaga ttgttaaaga tatattacag attaataata ttcttaaaat gtggtaattt
     6541 attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc cgcgcaacaa
     6601 aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta aaacgttcgt
     6661 ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc cagacgctgg
     6721 tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac cgtgcccata
     6781 ttgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg attgttattg
     6841 tgacgtcggg cgcgatcgcc gccggacgtg agcacctggg ttacccggaa ctgccagcga
     6901 ctatcgcctc gaaacaactg ctggcagcgg tagggcagag tcgactgatt caactgtggg
     6961 aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc cgtgctgata
     7021 tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg ctcgataaca
     7081 atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt aaagtcggcg
     7141 ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa ctgttgctgc
     7201 tgaccgatca aaaaggtttg tataccgctg acccgcgcag caatccgcag gcagaactga
     7261 ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac agcgtttcag
     7321 gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct tgccgtgcgg
     7381 gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat gtgatggaag
     7441 gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac cgtaaacgct
     7501 ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca actgccgcca
     7561 ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact ggcaatttct
     7621 cgcgtggtga agtcatccgc atttgcaacc tcgaaggccg cgatatcgcc cacggcgtca
     7681 gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa gaaattgatg
     7741 caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg attacccgtt
     7801 aaggagctgg ccgatgctgg aacaaatggg cattgctgcg aagcaagcct cgtataaatt
     7861 agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg atgaactgga
     7921 agcacaaagc gaaatcatcc tcaacgctaa cgcccaggat gttgctgacg cgcgagccaa
     7981 tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc tgaaaggcat
     8041 tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg taatcgatgg
     8101 cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg gggttattgg
     8161 cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt gcctgaaaac
     8221 cggtaatgcg gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg ctgcaacggt
     8281 ggcggtgatt caggacgccc tgaaatcctg cggcttaccg gcgggtgccg tgcaggcgat
     8341 tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat acatcgacat
     8401 gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt cgacaatccc
     8461 ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg tagagatcgc
     8521 tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat gtaatacggt
     8581 tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat taagcaaaca
     8641 aatggcggaa agcggcgtga cattacacgc agatgcagct gcactggcgc agttgcaggc
     8701 aggccctgcg aaggtggttg ctgttaaagc cgaagagtat gacgatgagt ttctgtcatt
     8761 agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata ttcgtgaaca
     8821 cggcacacaa cactccgatg cgatcctgac ccgcgatatg cgcaacgccc agcgttttgt
     8881 taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta ccgacggcgg
     8941 ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg cgcgtggccc
     9001 aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt acaccattcg
     9061 tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc cattgacgca
     9121 tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat gtcctttcag
     9181 ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg taggttcgac
     9241 tcctattatc ggcaccatta agaaaattaa taacttagct taacttctct aagaaattgt
     9301 atcttctgtt atcacttaca aacgcctata cgttgttatg ggctttggta gtgtaacacg
     9361 tatgggcgag tagttcccag gctcaacctc gtaacaagtt cctttgaagc tggaagacgt
     9421 gccaccgtct ctaaaccttc taccaaaact cttagcaaga tgttccacac ctggcgcggt
     9481 gtccaatgat gctacacgtc gtgagacgtt gcagaggata aacaggctaa atgatgggaa
     9541 gagttttaag tgcaatagtg gattaaagcc cacctcttca aaacctaact tgatcgggaa
     9601 agaatcaagc tctagcacgg tttcgtccac cggagtacaa cgggtgcctg ataagagaag
     9661 aactgctaag acttcatagc ttttgtgaaa aagccctttt tagtggatac gcctacggcg
     9721 cttatttttt ggtataacca tcaaaggcgc tttatggctc ctattgtctt gaaaatgtat
     9781 ctttacttgt attgtgtttt atgcgtagtc tccctgtgta gtatgtaact tgttaatttt
     9841 catatgaata cagaggcaca cgatgaaaaa cattgcagca atcaaacgta ataaccgcaa
     9901 gattcacgct cgtaagttcc tgtctacgcc agaaggaaaa gcctggctag agcgtaaaca
     9961 gagagagaac gaagaaagaa aactccttag tgagttgaaa tggcttagag aggatttctg
    10021 aaaatctcca gaacgcaaca ggacgcattc taacgctgtt ttcattactc ggtataggga
    10081 taggtgatgt gtgtttaatg cgtctgtgcg cttgttttgt gccttatttt gcattttcat
    10141 aatgcggaag agaacacgga aaaccttaaa attaattatt ggggtattta aggctatttt
    10201 caggtgattt aagggtatta aacttgatta aaatataaac agataattat gtatcttatt
    10261 gatttataag gttattttta agtaaaggta aataaaaaat aaatagccat aaatagctat
    10321 aaatacatct taatacctat atcccttccc ttatggctag attgcgagag gtgcttaacg
    10381 cgattttcag cgccccccga tctggaaaat ccagaatccc caaatatcag caaggaattt
    10441 tcttgcgtaa tcaacatcgt ttttacctat taaatcatta cacagaaagc ctttctgtaa
    10501 tgtctctcac taaactttca ctaaacacga gaatgatcgg ttttatgtcg gatcagatgc
    10561 tagaaacagc accacgtctc acaagggctg taagcgatga aaccagtgtt tatgcgggtg
    10621 caggtcaaaa tataggccaa aatccactta acataataaa tgttatatgt gctaaggaac
    10681 acatataacg ccttgaattg atgtatcagg ggaaaagtga tggatttttt acgtaggagc
    10741 acgaaagggc ttctattgac ttttgtatgc tatgaaatat aattaaagtt ttttgttttt
    10801 attgtgtgat gtggtgggtt acacagggga atagattaaa tggtgttctg tttatacagg
    10861 tttatttgtt agatcaacaa tttggcattt ctaaacagat gtttgaacgt tgccaaatgt
    10921 cttgacaaca caagaaaagg gcttgctatg ccaggcatta cacgaaataa ggctataaaa
    10981 acaaaataaa ttttaaaatt tgcacaaacg agaaactcgt tcagaaacaa gcagaacaat
    11041 ttttagccaa caatttctga tggtgctctg taagccgcat tacacaaggc tttgcgaaag
    11101 tgatgtaaaa gtgaagaaag taagaattga acttttaagc tacttttcat tggatttttt
    11161 agtagggaat tacacaggga atagagagca ttattctatg atttacacag ggaatatagg
    11221 gggattatgg cacacacata gaaagacatt acacagagtg ctatgtgaca aatggatgta
    11281 acgagaggtt acattcattt atctgaaatg ctctctgtaa gaaagttggt agtaacgata
    11341 tgttacatag actaattatt gaatgtaacg atattacaca tcggtagtaa cgatacgtta
    11401 catcggaaga tgattggatg taacggtagc tcacagtgaa ctaggggtag gtcatagtga
    11461 actaccccca cagaattctg ttacccctat caagtctgat acccctatca aaactgatac
    11521 ccgataagta taaataac
//
LOCUS       DAZPEP010000130        11507 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZPEP010000130 DAZPEP010000000
VERSION     DAZPEP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.130"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            460..1386
                     /locus_tag="ACHUT9_003292"
     CDS             460..1386
                     /locus_tag="ACHUT9_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFR1349697.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKETWRWVEGYNKATELARQCPDTHVLSISDREGDFYDLFERAEQTPGIKADWLV
                     RMKFNNRATLNINGKRDHRLLHERIMEITPQQLVEFTIPDGRGQQARTLPDMLSSDAG
                     PLSYPLIYSSKVMIISLFDSTVMPDKYRHNGK"
     gene            complement(1336..2529)
                     /locus_tag="ACHUT9_003293"
     CDS             complement(1336..2529)
                     /locus_tag="ACHUT9_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1349698.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="ACHUT9_003294"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="ACHUT9_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFR1349699.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="ACHUT9_003295"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="ACHUT9_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFR1349700.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="ACHUT9_003296"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="ACHUT9_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFR1349701.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="ACHUT9_003297"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="ACHUT9_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFR1349702.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="ACHUT9_003298"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="ACHUT9_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFR1349703.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="ACHUT9_003299"
     CDS             complement(11165..11308)
                     /locus_tag="ACHUT9_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349704.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2916 a         2892 c         2944 g         2755 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgaatc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tggcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaaaacaa aaaacgatca attagtattg
    11401 attcacataa tctctcatca ttaatgagaa cattgtcaca tatttacaat atagaactgt
    11461 tacttttagt aagctgttag taaccaactg ttactaataa ttttaat
//
LOCUS       DAZPEP010000131        11428 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZPEP010000131 DAZPEP010000000
VERSION     DAZPEP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.131"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUT9_003300"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="ACHUT9_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFR1349705.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUT9_003301"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="ACHUT9_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFR1349706.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUT9_003302"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="ACHUT9_003302"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFR1349707.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUT9_003303"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="ACHUT9_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFR1349708.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="ACHUT9_003304"
     CDS             complement(3928..5214)
                     /locus_tag="ACHUT9_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFR1349709.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="ACHUT9_003305"
     CDS             complement(5235..6026)
                     /locus_tag="ACHUT9_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFR1349710.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUT9_003306"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="ACHUT9_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFR1349711.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUT9_003307"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="ACHUT9_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFR1349712.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUT9_003308"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="ACHUT9_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFR1349713.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUT9_003309"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="ACHUT9_003309"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFR1349714.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUT9_003310"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="ACHUT9_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFR1349715.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="ACHUT9_003311"
     CDS             complement(9882..10364)
                     /locus_tag="ACHUT9_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFR1349716.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUT9_003312"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="ACHUT9_003312"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFR1349717.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2804 a         2748 c         3060 g         2816 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgtt ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagcac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccaa ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat tatcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggcggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctacc aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAZPEP010000132        11303 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZPEP010000132 DAZPEP010000000
VERSION     DAZPEP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.132"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            53..352
                     /locus_tag="ACHUT9_003313"
     CDS             53..352
                     /locus_tag="ACHUT9_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFR1349718.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(472..651)
                     /locus_tag="ACHUT9_003314"
     CDS             complement(472..651)
                     /locus_tag="ACHUT9_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFR1349719.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            725..2086
                     /gene="pabB"
                     /locus_tag="ACHUT9_003315"
     CDS             725..2086
                     /gene="pabB"
                     /locus_tag="ACHUT9_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFR1349720.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2090..2668
                     /locus_tag="ACHUT9_003316"
     CDS             2090..2668
                     /locus_tag="ACHUT9_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFR1349721.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUT9_003317"
     CDS             2852..4216
                     /gene="sdaA"
                     /locus_tag="ACHUT9_003317"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFR1349722.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4347..5945
                     /locus_tag="ACHUT9_003318"
     CDS             4347..5945
                     /locus_tag="ACHUT9_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFR1349723.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHUT9_003319"
     CDS             complement(5949..7505)
                     /gene="yoaE"
                     /locus_tag="ACHUT9_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFR1349724.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHUT9_003320"
     CDS             complement(7493..7651)
                     /gene="yoaL"
                     /locus_tag="ACHUT9_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFR1349725.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            7968..8939
                     /gene="manX"
                     /locus_tag="ACHUT9_003321"
     CDS             7968..8939
                     /gene="manX"
                     /locus_tag="ACHUT9_003321"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFR1349726.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKTLKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9002..9802
                     /gene="manY"
                     /locus_tag="ACHUT9_003322"
     CDS             9002..9802
                     /gene="manY"
                     /locus_tag="ACHUT9_003322"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFR1349727.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAIFWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9815..10666
                     /gene="manZ"
                     /locus_tag="ACHUT9_003323"
     CDS             9815..10666
                     /gene="manZ"
                     /locus_tag="ACHUT9_003323"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFR1349728.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10721..11179
                     /locus_tag="ACHUT9_003324"
     CDS             10721..11179
                     /locus_tag="ACHUT9_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFR1349729.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2783 a         2836 c         2838 g         2846 t
ORIGIN      
        1 taaggtcata tatagcctta tcgataacat ggttaatttt aaggataaga atatgcctgc
       61 tgtaatagat aaagccctgg atttcattgg tgccatggat gtatcagcgc caacaccaag
      121 ttcgatgaat gaaagcacgg cgaagggaat ctttaaatat ttaaaagaac tgggagtacc
      181 tgcgagtgcc gctgatatta cggcgcgagc tgaccaggaa ggctggaacc ctgggtttac
      241 ggaaaaaatg gttggatggg caaaaaaaat ggagtcaggt gaacgctctg tgattaaaaa
      301 tcctgaatac ttttcaacat atatgcagga agaactaaaa gcactggtct gagtaaaatt
      361 tacatcagca taaatggatc aggacgcttt taatcacata taaaaagcgt cctttttctc
      421 acaatcaaat aatcatcggc ttgtataacg aaatcaatac ggattagtgt gttactcgtc
      481 ttcatcctca aaacgcgcca cgattcgctc gccggaatgg ttggcacgca gctcttctgc
      541 caccaacgcg attgcctggc cgctgctcat cccttgtgcc atcagttcct ggatccgctc
      601 gacagctttt tgctgctgtt catgggtgag tgaaggtaaa cctgcaaaca ttgttaactc
      661 ctgctaaatt gttggcgcta attatttcat gctacccggc acatagccag tagagtcagg
      721 actgatgaag acgttatctc ccgctgtgat tactttaccc tggcgtcagg acgccgctga
      781 attttatttc tcccgcttaa gccacctgcc gtgggcgatg cttttacact ccggctatgc
      841 cgatcatccg tatagccgct ttgatattgt ggtcgccgat ccgatttgca ctttaaccac
      901 tttaggtaaa gaaaccgttg ttagtgaaag cgaaaaacgc acaacgacca ctgatgaccc
      961 gctacaggtg ctccagcagg tgctggatcg cgcagacatt cgcccaacgc ataacgaaga
     1021 tttgccattt cagggcggcg cactggggtt gtttggctac gatctgggcc gccgttttga
     1081 gtcactgcca gaaattgcgg aacaagatat cgttctgccg gatatggcag tgggtatcta
     1141 cgattgggcg ctcattgtcg accaccagcg tcatacagtt tctttgctga gtcataatga
     1201 tgtcaatgcc cgtcgggcct ggctggaaag ccagcaattc tcgccgcagg aagatttcac
     1261 gcttacttcc gactggcaat ccaatatgac ccgcgagcag tacggcgaaa aatttcgcca
     1321 ggtacaggaa tatctgcaca gcggtgattg ctatcaggta aatctcgccc agcgttttca
     1381 tgcgacctat tctggcgatg aatggcaggc attccttcag cttaatcagg ccaaccgcgc
     1441 gccatttagc gcttttttac gtcttgaaca gggtgcaatt ttaagccttt cgccagagcg
     1501 gtttattctt tgtgataata gtgaaatcca gacccgccct attaaaggca cgctaccacg
     1561 cctgcccgat cctcaggaag atagcaaaca agcagaaaaa ctggcgaact cagcgaaaga
     1621 tcgtgccgaa aatctgatga ttgtcgattt aatgcgtaat gatatcggtc gtgttgccgt
     1681 agcaggttcg gtaaaagtac cagagctgtt cgtggtggaa cccttccctg ccgtgcatca
     1741 tctggtcagc accataacgg cacaactacc agaacagtta cacgccagcg atctgctgcg
     1801 cgcagctttt cctggtggct caataaccgg ggctccgaaa gtacgggcta tggaaattat
     1861 cgacgaactg gaaccgcagc gacgtaatgc ctggtgcggc agcattggct atttgagctt
     1921 ttgcggcaac atggatacca gcattactat ccgcactctg actgccatta acggacaaat
     1981 atactgctct gcgggcggtg gaattgtcgc cgatagccag gaagaagcgg aatatcagga
     2041 aacttttgat aaagttaata agatattacg ccaactggag aagtaagacg tggaataccg
     2101 tagcctgatg cttgatgatt ttttatcgcg ctttcaactt ttgcgcccgc aaattaaccg
     2161 ggaaacccta aatcatcgtc aggctgccgt gttaatcccc atcgtccgtc gaccgcaacc
     2221 ggggttgttg ctgactcagc gttcaattca tctgcgtaaa cacgctggac aagtggcatt
     2281 ccctggaggt gcagtcgatg acacggacgc atcagttatc gccgccgcgc tgcgcgaagc
     2341 tgaagaagag gtcgctatac cgccttccgc cgttgaagtt atcggcgtgc tgccgcccgt
     2401 cgatagcgta actggctatc aggtaacccc agtggtcggc attatcccgc ccgatctgcc
     2461 gtatcgcgcc agtgaagatg aagtctcggc ggtgtttgaa atgccgctcg cccaggcatt
     2521 acatctgggt cgttatcacc ctttagatat ctaccgccgt ggcgattcac atcgggtatg
     2581 gctgtcctgg tacgaacagt attttgtatg gggaatgacc gcaggcataa ttcgtgagct
     2641 ggcgctgcaa attggtgtga aaccctgact atacttatct ttacatctac aaaacactac
     2701 ttgagacaat catcgcaata ttagttaaat cgcggttttt gattagttta attcatgtga
     2761 atagttaagc cagtcgccgc gttccctctt acactatgcg ctgttattag ttcgttactg
     2821 gaagtccagt caccttgtca ggagtattat cgtgattagt ctattcgaca tgtttaaggt
     2881 ggggattggt ccctcatctt cccataccgt agggcctatg aaggcaggta aacagttcgt
     2941 cgatgatctg gtcgaaaaag gcttactgga tagcgttact cgcgttgccg tggacgttta
     3001 tggttcactg tcgctgacgg gtaaaggcca ccacaccgat atcgccatta ttatgggtct
     3061 tgcaggtaac gaacctgcca ccgtggatat cgacagtatt cccggtttta ttcgcgacgt
     3121 agaagagcgc gaacgtctgc tgctggcaca gggacggcat gaagtggatt tcccgcgcga
     3181 caacgggatg cgttttcata acggcaacct gccgctgcat gaaaacggta tgcaaatcca
     3241 cgcctataac ggcgatgaag tcgtctacag caaaacttat tattccatcg gcggcggttt
     3301 tatcgtcgat gaagaacact ttggtcagga tgctgccaac gaagtgagcg tgccgtatcc
     3361 gttcaaatct gccaccgaac tgctcgcgta ctgtaatgaa accggctatt cgctgtctgg
     3421 tctcgctatg cagaacgaac tggcgctgca cagcaagaaa gagatcgacg agtatttcgc
     3481 gcatgtctgg caaaccatgc aggcatgtat cgatcgcggg atgaacaccg aaggtgtact
     3541 gccaggcccg ctgcgcgtgc cacgtcgtgc gtctgccctg cgccggatgt tagtttccag
     3601 cgataaactg tctaacgatc cgatgaatgt cattgactgg gtaaacatgt ttgcgctggc
     3661 agttaacgaa gaaaacgccg ccggtggtcg tgtggtaact gcgccaacca acggtgcctg
     3721 cggtatcgtt ccggcagtgc tggcttacta tgaccacttt attgaatcgg tcagcccgga
     3781 catctatacc cgttacttta tggcagcggg cgcgattggt gcattgtata aaatgaacgc
     3841 ctctatttcc ggtgcggaag ttggttgcca gggcgaagtg ggtgttgcct gttcaatggc
     3901 tgctgcgggt cttgcagaac tgctgggcgg tagcccggaa caggtttgcg tggcggcgga
     3961 aattggcatg gaacacaacc ttggtttaac ctgcgacccg gttgcagggc aggttcaggt
     4021 gccgtgcatt gagcgtaatg ccattgcctc tgtgaaggcg attaacgccg cacggatggc
     4081 tctgcgccgc accagtgcac cgcgcgtctc gctggataag gtcatcgaaa cgatgtacga
     4141 aactggtaag gacatgaacg ccaaataccg cgaaacctca cgcggtggtc tggcaatcaa
     4201 agtccagtgt gactaatact tcttactcgc ccatctgcaa cggatgggcg aatttatacc
     4261 cgctttctcg tctgctgtaa tattccccac tacacttcca ctgttgcgtc aggcgtttgt
     4321 cgccatacgc ttacagggtg gcccgcatgc aaaaagcaca acggatcatt aaaacctatc
     4381 gccgtaatcg aatgattgtt tgtacgattt gcgcactcgt tacgctcgct tcgaccctga
     4441 gcgtgcgatt tatttcacag cgtaacttaa atcaacaacg ggtagtacaa ttcgccaatc
     4501 acgctgtaga ggaattagat aaagtactgc ttcccctaca ggcaggtagc gaagtcttgc
     4561 ttccgctgat tggtctgccc tgctctgtcg cccatttgcc attacgtaaa caggcggcaa
     4621 aactccaaac tgtgcgatcc attggcctgg tgcaagacgg aacactctat tgctccagca
     4681 tttttggtta tcgcaatgtg cccgtcgtgg acattctggc tgaacttcct gcaccgcaac
     4741 cacttttacg cctgacgatc gaccgtgccc tgattaaagg cagtccggtt ttgattcaat
     4801 ggacgcctgc agcgggcagt agcaatgctg gggtcatgga gatgattaac atcgacttac
     4861 tgacggcaat gctccttgag ccacaactgc cgcaaatcag tagcgccagc ctgacggtgg
     4921 acaaacggca tttgctctat ggtaatgggc tggtagattc ccttccgcaa cctgaagaca
     4981 atgaaaacta tcaggtttct tcgcaacgct ttccttttac cattaacgtt aatggtccgg
     5041 gggctacggc gctggcatgg cactatcttc caacacaatt accgctggcg gtgctgctaa
     5101 gtttactggt gggctacatc gcctggctgg cgaccgctta ccggatgagc ttttcccgcg
     5161 aaatcaatct gggcctggcg caacatgagt tcgaattgtt ctgtcagcct ttgcttaatg
     5221 cgcgcagcca gcaatgtatt ggtgtagaga ttttgctgcg ctggaacaat ccgcgtcagg
     5281 gctggatttc accggatgtg tttattccta tcgcggaaga acatcattta attgtgccac
     5341 tgacccgcta tgtgatggca gaaaccattc gtcagcgcca tgttttcccg atgagtagtc
     5401 agtttcatgt tggcattaac gtcgcaccca gccattttcg ccgtggtgtg ctgataaaag
     5461 atctcaatca gtactggttt agcgctcacc cgattcagca actgatcctc gaaatcaccg
     5521 aacgcgatgc cttactggat gttgattatc ggattgcccg cgagctacat cgtaaaaacg
     5581 tcaaactggc gattgatgac ttcggcaccg gcaacagttc attttcctgg cttgaaacat
     5641 tacgtcctga catgctgaaa attgataagt catttaccgc agctataggt tctgacgcgg
     5701 ttaactcgac ggtgaccgat atcatcatcg cgctggggca aagactgaat attgaactgg
     5761 tggcggaggg cgtggaaacg caagaacagg cgaagtattt gcgccgtcat ggcgtgcata
     5821 ttttgcaagg gtatttgtac gcacagccga tgccgctacg tgattttccc aaatggctgg
     5881 cgggcagcca accgccgccc gcccggcata atggacatat cacgcccatt atgccgttac
     5941 gttaatgctt actcatcttc atcgtgcgcc ggttgctctt taacaatgcg aaccagatca
     6001 acacgataat cattggcttc aatgatggtg atatgcagtg gccctacatc aatcacatcg
     6061 cccacacggg gaatgtgacc atttgccgag atcacgaggc ccgcgaccgt cgcgatatca
     6121 tcgtcatcgg caaggtgctc aacatcaagc gcctgctgca aggcatgcaa atctgtaccg
     6181 ccttttacca gccagccgtc accatcagta atgatttccg gcgtttcgtc agcgtccggg
     6241 aattcacccg caatggcttc cagcacatcc agcggcgtga ccagaccttg taccacacca
     6301 aactcgttgg tcacgataac aaagctcccg cgagcacgac gcagcacgcc caacaggttg
     6361 atcggatcga gggtttccgg gacgataatc gccggagacg ccgaagcaat cgccgccaca
     6421 tcaacgccct cttccagcgc caccagcagt tctttagcac gtacaatacc gatgatttca
     6481 tccagttcac cgcgacatac cgggaacaga ctgtgcggtg aagagagcag ttgctcgcgg
     6541 atttcatcga ccccgagatt agcgtcaacc cagcttattt caccgcgcgg cgtcatgatc
     6601 ccgcgcagag aacgcgacgc aagcgtcagt acgccgttaa tcatgtaacg ttcttcttcg
     6661 gcaaatgcac cttccgggat cggcatcggc atcgggttat cggcatcgtg ctgaacattg
     6721 gcctgacgtt tcccgcccat caaacgcagg atggcatcgg cagtacgcgc tcgcagcggc
     6781 aaagtcgact ggtggcgaat aaagttgcga cgcgcaatct ggttaaacac ttcgatgatg
     6841 atcgagaagc caatcgcggc atacaggtaa cctttcggaa tgtggaaacc gaaaccttct
     6901 gccaccagac tcagaccaat cattaacagg aagctcagac agagcaccac caccgtgggg
     6961 tgctggttaa cgaatcgcgt cagcggtttg gatgccagca acataaccgc catcgcaatc
     7021 actaccgccg ccatcatcac cggcagatgg ttaaccatcc ctactgcagt aattaccgca
     7081 tccaacgaga agacggcgtc aaggatgaca atctgtgtga cgaccaccca gaaactggcg
     7141 tagcctttac cgtggccgga atcatgatcg cggttttcca gccgttcatg cagttcggtt
     7201 gttgctttga acagcaagaa tatccccccg aacaacataa tcaggtcgcg tccggagaag
     7261 gagaaatcca tgacggtaaa tagcggtttg gtcagcgtga ccatccatga aatcagcgac
     7321 agcagcccca gacgcataat cagcgccagt gataacccca gcaaacgcgc tttatcgcgt
     7381 tgttttggcg gcagtttgtc agcaagaatg gcgatgaaga ccaggttatc gatacccagc
     7441 acaatttcga gaacaacaag cgtgagtagc cccgcccaaa ttgaggggtc cattaagaat
     7501 tccatgacaa gctcctgctt aaggaatagc tattcgacgc cagaaataat gcaggcgtaa
     7561 cgacaaaatg caaacgaaag gtgcggcata gagtgccaga aaggcaggcg ttaaaaggcc
     7621 tgatgctgaa atgacgtcgg tgacgatcca tactgcgggc tactgcccta tactccatgg
     7681 ttgttaaacg ggagttaaac atatcagaga cgcctctgat ttggcaaaga tttaccttcc
     7741 tttgcaaacg aatgtgacaa ggatatttta cctttcgaaa tttctgctaa tcgaaagtta
     7801 aattacggat cttcatcaca taaaataatt ttttcgatat ctaaaataaa tcgcgaaacg
     7861 caggggtttt tggttgtagc ccttatctga atcgattcga ttgtggacga cgattcaaaa
     7921 atacatctgg cacgttgagg tgttaacgat aataaaggag gtagcaagtg accattgcta
     7981 ttgttatagg cacacatggt tgggctgcag agcagttgct taaaacggca gaaatgctgt
     8041 taggcgagca ggaaaacgtc ggctggatcg atttcgttcc aggtgaaaat gccgaaacgc
     8101 tgattgaaaa gtacaacgct cagttggcaa aactcgacac cactaaaggc gtgctgtttc
     8161 tcgttgatac atggggaggc agcccgttca atgctgccag ccgcattgtc gtcgacaaag
     8221 agcattatga agtcattgca ggcgttaaca ttccaatgct cgtggaaacg ttaatggctc
     8281 gtgatgatga cccaagcttt gatgaactgg ttgcgctggc agtagaaaca ggccgtgaag
     8341 gcgtgaaaac actgaaagcc aaaccggttg aaaaagccgc gccagcaccc gctgccgcag
     8401 caccaaaagc ggctccaact ccggcaaaac caatggggcc aaacgactac atggttattg
     8461 gccttgcgcg tatcgacgac cgtctgattc acggtcaggt cgccacccgc tggaccaaag
     8521 aaaccaatgt ctcccgtatt attgttgtta gtgatgaagt ggctgcggat accgttcgta
     8581 agacactgct cacccaggtt gcacctccgg gcgtaacagc acacgtagtt gatgttgcca
     8641 aaatgattcg cgtctacaac aacccgaaat atgctggcga acgcgtaatg ctgttattta
     8701 ccaacccaac agatgtagag cgtctcgttg aaggcggcgt gaaaatcacc tctgttaacg
     8761 tcggtggtat ggcattccgt cagggtaaaa cccaggtgaa taacgcggtt tcggttgatg
     8821 aaaaagatat tgaggcgttc aagaaactga atgcgcgcgg tattgagctg gaagtccgta
     8881 aggtttccac cgatccgaaa ctgaaaatga tggatctgat cagcaaaatc gataagtaac
     8941 gtattgtgtt gattatcact cagttttcac acttaagtct tacgtaaaca ggagaagtac
     9001 aatggagatt accactcttc aaattgtgct ggtatttatc gtagcctgta tcgcaggtat
     9061 gggatcaatc ctcgatgaat ttcagtttca ccgtccgcta atcgcgtgta ccctggtggg
     9121 tatcgttctt ggggatatga aaaccggtat tattatcggt ggtacgctgg aaatgatcgc
     9181 gctgggctgg atgaacatcg gtgctgcagt tgcgcctgac gccgctctgg cttctatcat
     9241 ttctaccatt ctggttatcg caggtcatca gagcattggt gcaggtatcg cactggcaat
     9301 ccctctggcc gctgcgggcc aggtactgac catcatcgtt cgtactatta ccgttgcttt
     9361 ccagcacgct gcggataagg ctgctgataa cggcaacctg acagcgattt tctggatcca
     9421 cgtttcttct ctgttcctgc aagcaatgcg tgtggctatt ccggccgtca tcgttgcgct
     9481 gtctgttggt accagcgaag tacagaacat gctgaatgcg attccggaag tggtgaccaa
     9541 tggtctgaat atcgccggtg gcatgatcgt ggtggttggt tatgcgatgg ttatcaacat
     9601 gatgcgtgct ggctacctga tgccgttctt ctacctcggc ttcgtaaccg cagcattcac
     9661 caactttaac ctggttgctc tgggtgtgat tggtactgtt atggcagtgc tctacatcca
     9721 acttagcccg aaatacaacc gcgtagccgg tgcgcctgct caggcagctg gtaacaacga
     9781 tctcgataac gaactggact aacaggtgag cgaaatggtt gatacaactc aaactaccac
     9841 cgagaaaaaa ctcactcaaa gtgatattcg tggcgtcttc ctgcgttcta acctcttcca
     9901 gggttcatgg aacttcgaac gtatgcaggc actgggtttc tgcttctcta tggtaccggc
     9961 aattcgtcgc ctctaccctg agaacaacga agctcgtaaa caagctattc gccgtcacct
    10021 ggagttcttt aacacccagc cgttcgtggc tgcgccgatt ctcggcgtaa ccctggcgct
    10081 ggaagaacag cgtgctaatg gcgcagagat cgacgacggt gctatcaacg gtatcaaagt
    10141 cggtttgatg gggccgctgg ctggtgtagg cgacccgatc ttctggggaa ccgtacgtcc
    10201 ggtatttgca gcactgggtg ccggtatcgc gatgagcggc agcctgttag gtccgctgct
    10261 gttcttcatc ctgtttaacc tggtgcgtct ggcaacccgt tactacggcg tagcgtatgg
    10321 ttactccaaa ggtatcgata tcgttaaaga tatgggtggt ggcttcctgc aaaaactgac
    10381 ggaaggggcg tctatcctcg gcctgtttgt catgggggca ttggttaaca agtggacaca
    10441 tgtcaacatc ccgctggttg tctctcgcat tactgaccag acgggcaaag aacacgttac
    10501 tactgtccag actattctgg accagttaat gccaggcctg gtaccactgc tgctgacctt
    10561 tgcttgtatg tggctactgc gcaaaaaagt taacccgctg tggatcatcg ttggcttctt
    10621 cgtcatcggt atcgctggtt acgcttgcgg cctgctggga ctgtaagact gttgtacact
    10681 accggggcct tttggccccg tttttttatc tggaggatta atgacaatca cggacctggt
    10741 actgattctt ttcatcgccg cactcctggc cttcgcgatc tacgatcagt tcatcatgcc
    10801 ccgccgtaac ggccccaccc tgctggcaat tcctttgctc cggcgtggtc gcatcgatag
    10861 cgttatcttc gtcggattga ttgtcattct tatctataac aacgtcacga atcatggtgc
    10921 gttaataacg acatggttat taagcgcact ggctctgatg ggtttttata tattctggat
    10981 ccgcgttccg aagatcatct ttaaacaaaa aggttttttc ttcgccaatg tctggattga
    11041 atatagccga atcaaagcga tgaacttgtc ggaagatggc gtgttggtga tgcaattaga
    11101 acagcgtcgg ctgttaatcc gcgttcgaaa tatcgacgat ctggaaaaaa tttataagct
    11161 tctcgtttca actcaataag ttatgaattt agccaaagct atgtttagtg tatttttaat
    11221 aatcagacat agcttaggct atattacctc ttcccttatt tgttatttat tttaacgttt
    11281 cattgatata taaatccaaa tga
//
LOCUS       DAZPEP010000133        11297 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZPEP010000133 DAZPEP010000000
VERSION     DAZPEP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.133"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            79..213
                     /locus_tag="ACHUT9_003325"
     CDS             79..213
                     /locus_tag="ACHUT9_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1349730.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA
                     "
     gene            192..296
                     /gene="speFL"
                     /locus_tag="ACHUT9_003326"
     CDS             192..296
                     /gene="speFL"
                     /locus_tag="ACHUT9_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFR1349731.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            607..2805
                     /gene="speF"
                     /locus_tag="ACHUT9_003327"
     CDS             607..2805
                     /gene="speF"
                     /locus_tag="ACHUT9_003327"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFR1349732.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2802..4121
                     /gene="potE"
                     /locus_tag="ACHUT9_003328"
     CDS             2802..4121
                     /gene="potE"
                     /locus_tag="ACHUT9_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFR1349733.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHUT9_003329"
     CDS             complement(4177..5817)
                     /gene="pgm"
                     /locus_tag="ACHUT9_003329"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFR1349734.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHUT9_003330"
     CDS             complement(5843..6388)
                     /gene="seqA"
                     /locus_tag="ACHUT9_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFR1349735.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHUT9_003331"
     CDS             6573..7337
                     /gene="ybfF"
                     /locus_tag="ACHUT9_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFR1349736.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHUT9_003332"
     CDS             7477..7770
                     /gene="ybfE"
                     /locus_tag="ACHUT9_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFR1349737.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7910..8440
                     /gene="fldA"
                     /locus_tag="ACHUT9_003333"
     CDS             7910..8440
                     /gene="fldA"
                     /locus_tag="ACHUT9_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFR1349738.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8650..8736
                     /gene="uof"
                     /locus_tag="ACHUT9_003334"
     CDS             8650..8736
                     /gene="uof"
                     /locus_tag="ACHUT9_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFR1349739.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8729..9175
                     /gene="fur"
                     /locus_tag="ACHUT9_003335"
     CDS             8729..9175
                     /gene="fur"
                     /locus_tag="ACHUT9_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFR1349740.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHUT9_003336"
     CDS             complement(9260..9586)
                     /gene="chiQ"
                     /locus_tag="ACHUT9_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFR1349741.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHUT9_003337"
                     /pseudo
     CDS             complement(9636..11042)
                     /gene="chiP"
                     /locus_tag="ACHUT9_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         2764 a         2779 c         2810 g         2944 t
ORIGIN      
        1 tcttaacgca aatataaaac acgaaattac aacatttaag taacaaaaca gcatctaata
       61 tggcgatatt cataattaat gatttattat ctggggtatg atcgtttttt gttgatcttc
      121 ttcacagatt atagccattt catggataga ataactctac cttcaactga cacagcaaga
      181 ggtaaaggta aatggaaaat aataaccgct taatgcctca tataaggcgg acaacccata
      241 tcatgaagtt tgcccatcgt aatagttttg actttcattt ctttaatgcc cgctagtctt
      301 ctgactaaag ggcaccccaa cgtacaggtc tccctgactt taagcattac aggttaatac
      361 ctgtattcct cggtgctcat atactgctaa cccttttaaa ctctaaataa ttcgagtcgc
      421 agcacttgca acttgaggta tgacgagtat agccagttac cgggctggtc tgggttattg
      481 catctgcaaa aagcaaacta ctgatttatt tatcagcggt ggagctttgc tttttttccg
      541 gcgtgatcga tttctccttt gagaaattga ggacctgcta ttacctgaaa taaagagatg
      601 aataaaatgt cagaattaaa aattgccgtt agccgttctt gcccggattg tttttccact
      661 catcgtgcat gcgtgaatat tgacgaaagt aattatattg acgttgccgc cattatttta
      721 tcagtcaatg atgttgaacg tggaaaactc gatgaaatag acgctactgg ctatggcatt
      781 cctgttttta ttgcaacgga aaatgaagaa cgtgtaccag cagaatatct gccacgtatt
      841 tctggtgtct tcgagcattg cgaatcacgg aaagagtttt atggacgtca gttagaaacc
      901 gctgctagcc attatgaaac gcaactgcgc ccgcccttct tccgcgcact ggtcgattat
      961 gtcaatcagg gcaatagcgc ttttgactgc ccgggtcatc agggaggtga gtttttccgt
     1021 cgtcatccgg cagggaatca gttcgtggag tacttcggag aaacgctgtt ccgttccgac
     1081 ttatgcaacg cagacgtagc aatgggcgat ctgctgattc acgaaggcgc gccatgcatt
     1141 gcacagcaac atgcggcaaa agtgtttaat gccgataaaa catacttcgt tctaaatggc
     1201 acttcatctt ctaacaaagt ggttttaaac gccctgctaa caccgggtga tctggtgctg
     1261 tttgatcgca ataaccacaa atctaaccac cacggagcgt tgctacaggc tggtgcaaca
     1321 ccggtttatc tggaaacggc acgtaacccg tatggcttta tcggtggcat tgatgcgcac
     1381 tgttttgaag aaagttacct gcgtgagctg atcgcggaag tcgcaccgca gcgggcaaaa
     1441 gaggctcgtc ctttccgcct cgctgtgatt cagttaggca cctacgacgg tacgatttat
     1501 aacgcccgcc aggtggtgga taaaattggt catctgtgtg actacattct gtttgactcc
     1561 gcatgggttg gctatgagca atttatcccg atgatggctg actgttcgcc gctgttgctg
     1621 gatcttaatg agaacgatcc gggtattctg gttacgcaat ccgtacataa acagcaggct
     1681 ggtttttctc aaacttcaca aatccataaa aaagacagcc acatcaaagg gcaacagcgt
     1741 tatgtaccgc acaaacgcat gaacaacgcc tttatgatgc acgcctccac cagcccgttc
     1801 tatccgctgt ttgccgcact ggatatcaac gccaaaatgc atgaaggtgt cagcggtcgt
     1861 aatatgtgga tggattgtgt ggtaaatggc attaatgccc gcaaactgat cctcgataac
     1921 tgtcagcata ttcgtccgtt cgtacctgaa ctggtggatg gtaaaccctg gcagtcgtat
     1981 gaaacagcgc aaattgcggt tgatctgcgc ttcttccagt ttgtaccagg ggaacactgg
     2041 cattcttttg aaggctatgc agagaatcaa tactttgtcg atccttgcaa actgttgctg
     2101 acaaccccag gtattgatgc acgtaacggc gaatatgaag cgttcggtgt acccgcgacg
     2161 attcttgcta acttcctgcg cgaaaatggc gtagtgccgg aaaaatgcga tcttaactcc
     2221 atcctcttcc tgctgactcc ggcagaagat atggccaaac ttcagcaact tgttgccctg
     2281 ctggtacgct tcgaaaaact gcttgagtcc gacgcgccat tagcagaagt gctgccttcc
     2341 atctacaaac agcatgaaga gcgctacgcc ggttataccc tgcgtcagtt gtgccaggaa
     2401 atgcatgatt tgtatgcccg acacaacgtg aaacaactgc aaaaagagat gttccgcaaa
     2461 gcgcacttcc cacacgtcag catgaatccg caagaagcca actacgccta tttacgcggt
     2521 gaagtggagc tggttcgtct gccggatgcc gaaggtcgta tcgctgccga aggggcgctt
     2581 ccttatcctc cgggtgtgct gtgtgttgtt ccgggtgaaa tctggggtgg tgctgttttg
     2641 cgttacttca gcgctctgga agaagggatc aacctgctgc caggttttgc accggagctg
     2701 cagggtgtct atatcgaaga acatgatggt cgtaagcaag tttggtgcta tgtcatcaag
     2761 cctcgtgatg cgcaaagcac cctgttgaaa ggggaaaaat tatgagtcag gctaaatcga
     2821 acaaaatggg cgtcgttcag ttaaccatac tgacgatggt caacatgatg ggctccggta
     2881 tcatcatgct gccgacaaag cttgccgaag ttgggacaat ttcaattatc tcctggctgg
     2941 tgacagccgt aggctcaatg gcactggcat gggcattcgc aaaatgcggt atgttcagcc
     3001 gtaaatcagg tggtatgggc ggttatgccg agtatgcttt cggtaaatcc ggtaacttta
     3061 tggcgaacta tacctacggc gtctcactgc tgattgctaa cgtcgcgatt gctatttcgg
     3121 cggttggtta cggcaccgaa ctgctcggcg cgagtttgtc gccagtgcag attggtcttg
     3181 cgaccatcgg ggtgctgtgg atttgtaccg tggctaactt tggtggtgcg cgcattaccg
     3241 ggcaaatcag tagcattacc gtgtgggggg tcattattcc ggtggtaggc ctgtgcatta
     3301 tcggctggtt ctggtttagc ccgacgcttt acgttgattc ctggaatccg catcatgcac
     3361 cgttcttcag tgcagtaggt tcttccatcg ctatgacgct gtgggctttt ctcggtctgg
     3421 agtctgcgtg tgcgaatact gatgtagtgg aaaacccgga acgtaatgtg ccaatcgcgg
     3481 tactcggcgg tacgttaggt gcggcggtga tttatatcgt ctccaccaac gtgattgcag
     3541 ggattgtgcc taatatggag ctggcaaatt caacggcacc atttggcctg gccttcgcac
     3601 agatgttcac accagaagtg ggtaaagtca ttatggcgct gatggtgatg tcctgctgcg
     3661 gttcgctgct tggctggcag ttcaccattg cccaggtgtt taagtcttca tctgatgaag
     3721 gctacttccc taaaattttc tcccgtgtaa ccaaagtgga tgcaccggtg cagggaatgt
     3781 tgaccattgt gattatccag agcgggttgg cgctgatgac gattagcccg tcgctaaaca
     3841 gtcagttcaa cgtgctggtt aacctggccg tggtgaccaa tatcattccg tatattctgt
     3901 caatggcggc attagtcatt attcagaagg tcgctaatgt gcccccctca aaagcgaaag
     3961 ttgcaaactt tgttgctttt gttggcgcga tgtatagctt ttatgcgctg tactcatccg
     4021 gggaagaagc catgctgtac ggttccatcg tgaccttcct tggttggaca ctgtatggtc
     4081 tggtatcacc acgctttgaa ctgaaaaata aacacggtta aaaaagcaaa gtaaaaaagg
     4141 gcgatcttgc gaccgccctt tttttattaa atgtgtttac gcgtttttca gaacttcgct
     4201 aacaatctca accgcttctt tctcaatctg cttgcgatgt tcttcaccga ggaagctttc
     4261 gcagtagatc ttatatgcgt cttccgtgcc tgacggacgc gcggcgaacc agccgttgtc
     4321 agtcatcact ttcagaccgc cgatagaagc accattgccc ggtgccgccg tcaggcgcgc
     4381 ggtgatcggg tcgcctgcca gggtgctggc gctcaccatt tccggagaca gcttagacag
     4441 cgccgctttt tgtgcggaag tcgcagctgc ctgcaaacgg ttgtagctcg gcgcaccaaa
     4501 gcgttttgcc agttcgttgt agtgttcctg cgggttctta ccggtgacag cggtgatttc
     4561 cgccgccagc agacacatga tgatgccgtc tttgtcggtg gaccacggcg tgccgtcgaa
     4621 acgcaggaag gaagcccctg cgctctcttc gccgccaaag ccgaagctgc cgtcgaacag
     4681 accatcaaca aaccatttga aacctaccgg gacttctacc agcttgcggc ccaagtcatt
     4741 gaccacacgg tcgatcatcg cagaagaaac cagcgtttta ccgacagcaa catctttgcc
     4801 ccactgcgga cgatgctgga acaggtagtt gatcgccacc gccaggtagt ggttcggatt
     4861 catcaaacct gccggagtga cgataccgtg acggtcataa tccgggtcgt tagcaaacgc
     4921 cagatcgaac ttatcacgca atgccagcag gcccgccatc gcacactcgg aggagcagtc
     4981 catacggatc gcgccgtctt tatcgaggtg cataaagcgg aaggtttgat cgacctgatc
     5041 gttaacgatg gtcaggttga ggttgtaata ctcgccaatt cgcttccagt attcgatacc
     5101 ggaaccaccc agcggatcaa cgcccagcgt caggcccgtt ttctgaatcg ccgccatatc
     5161 aacgatatcg gccagacctt ccacaaactg ctgcaccaga tcctgctctt tcacatgacc
     5221 ggatgccatc gcttcgtcga gggagatacg cttcacgcct ttcaggccat cggccatcag
     5281 tgcgttggca cgatcttcca ccactttagt gacgttggta tcagccgggc caccatttgg
     5341 cggattgtac ttgataccac catcttccgg cgggttatgg gagggtgtaa tcacgatacc
     5401 gtctgccagc gggccacctt ttttattatg aaccaggatg gcattggaaa cggcaggcgt
     5461 cggagtgaag ccattgtttt cctgcacaat gacatcaacg ccgttcgctg ccagcacttc
     5521 cagtacggaa atgaacgcag gctcggacag ggcgtgagta tctttaccca catagcaagg
     5581 gccagtgatg ccgtttttcg cacgttcttc agcaattgcc tgagcgatcg ccagaatgtg
     5641 cggctcgtta aagctgtggc gcgctgcact gccacggtga ccggaagtac cgaatttcac
     5701 cgcgtgctcc gcattccctg cttctggttt cagtacataa tattgcgccg tcagttgggc
     5761 gacgttaatc aaatcactct gttgtgcagg ttggcctgca cgattgtgga ttgccattgc
     5821 tttgtccttt gtctgcaacg ttttagatag ttccgcaaac cttctcaatc aattccgccg
     5881 ggaattgcat cgactgcatg atgtgttcga tcatgctgca tttacggccg gtgttggtgt
     5941 tggtgatcac ccaatacggc gtgcctggca catgtttcgg cttggtctga ttaccatttt
     6001 tcagcagcgt ttgttcatct gccgcaaagt aaacgcgtgt acgaccgtgc aacgattccg
     6061 ttgcttcggc aaacgcctgg gcgtcaagag aatatagtgt agacaacagc agcataaagc
     6121 gattgaccgc tcgcttttgc tctgcgtatt cgtcagaaag cagaagttca cgcattgcgc
     6181 gaaccttgtc tttaatcgtt ttgaccggct tcgcttcgac gatagcaggt gacgcaacgc
     6241 gaacctcttt cgtcaccgga gcagcaggct gtgatgcggc ggaaaatttc aacatacgcc
     6301 gtaaaatgtc ggatgcgctc tcgccgatat gcttagtgtg gctggcaata tagctgtaga
     6361 gttcatcatc aacttcaatc gttttcatct taatccagta cagtgtctta gctgaataca
     6421 agtcgccaag agtatatgaa taaatcccgg cagcggatag cgtcaagcct ggcttgcaga
     6481 aaaactcgga ataaacctta tttgttgcag ccctgtggca caatggtcaa cataataccc
     6541 taacccgaca acgcgaaaaa aagaactttg ccatgaaatt gaatatccgc gcgcaaactg
     6601 cacaaaacca gcacaataat tctcccatcg ttcttgtcca tggtctgttt ggtagcctcg
     6661 acaaccttgg cgtactggct cgcgatctgg taaacgatca caatatcatc caggttgata
     6721 tgcgtaacca cggtctttca ccgagagatc cggtaatgaa ttacccggcg atggcgcagg
     6781 atcttgttga tactctggat gcacagcaga tcgacaaagc aacatttatc ggtcactcca
     6841 tgggcggtaa agcggtaatg gcacttacgg cactggcccg cgatcgcatc gataaactgg
     6901 tggcgatcga tatcgcaccg gtcgactatc acgtacgccg tcatgatgag attttcgcgg
     6961 ctatcaacgc ggtcagtgaa tcggacgcac aaactcgcca gcaagcagca gcaataatgc
     7021 accagcatct taatgaagaa ggggtgattc agtttctgtt gaaatctttt gttgacgggg
     7081 agtggcgctt taacgtgccg gtattgtggg atcagtatcc gcatattgtg ggttgggaga
     7141 aaatcccggc atgggatcac cctgccctgt ttatccctgg cggcaattct ccgtatgtta
     7201 gcgagcagta ccgtgatgat ttactggcac aatttccaca ggcacgggcg catgtgattg
     7261 caggcgcggg tcactgggtc catgctgaaa aaccggatgc ggtattacgc gctatccgtc
     7321 gctatctcaa tgattaacgc aactgattaa aaacccagcg tccgcgtcgc tttccgcgcg
     7381 tgggcgttgg cgtgactcat cgcgctgatg tattatggcg cgctatccat ccgggcagag
     7441 gcctggctga ttgtttcccc cgaagtcacc aagatcatgg ccaaagaaca aacggaccgt
     7501 acgacattag atctgttcgc gcacgagcgt cgaccgggac gaccgaaaac taatccgctt
     7561 tcgcgcgatg aacagctgcg tattaataaa cgcaaccagc taaaacgcga caaagtacgt
     7621 ggccttaagc gtgtcgaact gaagctgaac gcggaagctg tcgaggcgct gaacgagctg
     7681 gcggagtcgc gcaatatgag ccgtagcgaa ctgatcgaag agatgttaat gcagcaactg
     7741 gcggcgctgc gtagtcaggg catcgtttaa atttccactt tcatgtagca cagtgtgcag
     7801 tcctgctcgt ttgctgattc cgcaagactg cctgttctgc tatgattgcc tttatccgtg
     7861 ggcaattttc cacccccatt tcaataagtt tcaagaggtt atttcactca tggctatcac
     7921 tggcatcttt ttcggcagcg acaccggtaa taccgaaaat atcgcaaaaa tgattcaaaa
     7981 acagcttggt aaagacgttg ccgatgtcca tgacattgca aaaagcagca aagaagatct
     8041 ggaagcttat gacattctgc tgctgggcat cccaacctgg tattatggcg aagcgcagtg
     8101 tgactgggat gacttcttcc cgactctcga agagattgat ttcaacggca aactggttgc
     8161 gctgtttggt tgtggtgacc aggaagatta cgccgaatat ttctgcgacg cattgggcac
     8221 catccgcgac atcattgaac cgcgcggtgc aacgatcgtt ggtcactggc caactgcggg
     8281 ctatcatttc gaagcatcaa aaggtctggc tgatgacgac cactttgtcg gtctggctat
     8341 cgacgaagac cgtcagccgg aactgaccgc tgaacgtgta gaaaaatggg ttaaacagat
     8401 ttctgaagag ttgcatctcg acgaaattct caatgcctga tgtgatgcgg cgtagactca
     8461 tgtctacgcc gtattaatag ataatgccaa tcaaaataat tgctacaaat ttgtaacttt
     8521 tgctgttgta cctgtacaat gtcccggtgt tcaagtggcc ttgccgttgt aaatgtaagc
     8581 tgtgccacgt ttttattaac aatatttgcc agggacttgt ggttttcatt taggcgtggc
     8641 aattctataa tgatacgcat tatctcaaga gcaaattctg tcacttcttc taatgaagtg
     8701 aaccgcttag taacaggaca gattccgcat gactgataac aataccgccc taaagaaagc
     8761 tggcctgaaa gtaacgcttc ctcgtttaaa aatcctagaa gttcttcagg agccggacaa
     8821 ccatcacgtc agtgcggaag atttatacaa acgtctgatc gatatgggtg aagaaattgg
     8881 tctggctacg gtatatcgcg tactgaacca gtttgacgac gctggtatcg tcacccgcca
     8941 caattttgaa ggcggtaaat ccgtatttga actgacacag caacatcacc acgatcacct
     9001 gatctgcctc gactgcggca aggttatcga atttagtgat gattccatcg aagcgcgtca
     9061 gcgtgaaatt gccgcaaaac atggcattcg cctgactaac cacagtctct atctttacgg
     9121 tcactgtgcc gaaggcgatt gccgcgaaga tgaacatgcg cacgaaggca aataagccag
     9181 cctgaatgag aaaaagccaa cctgcgggtt ggctttttta tgcaagggaa ataagaaaag
     9241 cgagagttac agctctcact tatttgttat tactgcgaat ttcctgccaa actttatcac
     9301 aatcggcttt caccgcctga tcatttccgg tttgcgttgc acgcagacac tgctgataat
     9361 ccagtacgcg gacactttcc tggtcggcaa aatgctgatg ttgtttctct ttcttcagca
     9421 cgtttaacac gctctggcag gcttcaattt tttctggcga accttgtgcg gtgttgatac
     9481 aggcgctgta tgcctctttc agacgactgt cttcctgtgg cgcggttgat tgcgcacaag
     9541 ctaccagccc cgatgccatt atggcgatga gaatcagttt tttcatagca aattcctcaa
     9601 acgtgcggca ggcacaaaac ctgccgcgtc gggcatcaga agatggtgaa tggtgcgatt
     9661 accataaatt tcacgtcacg ctcatcctgg aagatgttgc cgtaaccacc gccccagctt
     9721 gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt tgaacatcgt gcctttggcg
     9781 cgaccgtcct gaatggtgta gaccgcatcc aggctgtatg cagactcttc aatagtccgg
     9841 tttttgtcgt agtacgcatc cggattgctc tgctaggtcg caggtttagc atcccatgcg
     9901 taaacgtagg aagcgccgat ggcgaagcct ggaagattcc agtttttcag gtcatacatc
     9961 gcaccgaaga agaccgcttt ttcgccgtta gcgttgaagt cggaacggtt atcccaccag
    10021 atatccaggc ggccgttgga ggaagcgtag gttggagtca tacgttgcag gaagtatccc
    10081 tgctgaccgt cagccttaac ccaggtgcct tcgaggcgca aatccactac gtcagccgcc
    10141 cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt cataaaggtc gttgacgctg
    10201 cgatcgtcaa ctttatcgcg cgtaccatag aactggtagc tggtggttaa cgggctaccg
    10261 gcgatatcaa atttgtagct ggctttggca aaatattgat cgatataccc ttccgcctga
    10321 ccaaatgccg cttccagtac gaagttattt ttgaagtcgt atttcgcccc aagggagtgc
    10381 agataatcaa ctttggtggt tttatcgttc tgataaaact catccatttc cagatgccac
    10441 ggcgctttgt attcgttggt ccacatgtag gagaaactca acgcaccagc atcgccgtaa
    10501 tcaaaattcg ccccggcttc cgccccctga taagtacctg gcataaagct ccagtgcggc
    10561 gctaacagcg tttgaccagt tggctgaatg taacctgccc tcgcccaaac cggaccgtat
    10621 ttaaatttgg ccgcagcttt atacaggctt ataccgcttt tgtcgccgga ccagtcttcg
    10681 tcataggctt tattactttt tgaaaacgcg atttcgttcg ggtgggagct gtcgccgttt
    10741 tccgccattt caatcgccgt aaacgcagca atatcaaggc cgaacatatc agcagcataa
    10801 ccggactgaa aatcgaggtt ggcattccag gtggagtgag aaaggttggt tttgtatttg
    10861 tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc agtaatatat accgccggtt
    10921 aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg gcattgccat aaagcccgac
    10981 attgctgtaa caccggcgat agccagcgcc agcgtactat gtttgccact aaacgtacgc
    11041 atgggttaat cctctttgac gtataaattg ctgcaccaaa ggtgaacagc gaaaaaataa
    11101 aaattaaaaa agtagcgttg agaaaagtct cgctgactac aggaacagac tatttttcgc
    11161 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    11221 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    11281 agcgtgaaat tccagca
//
LOCUS       DAZPEP010000134        11295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZPEP010000134 DAZPEP010000000
VERSION     DAZPEP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.134"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..741
                     /gene="hdhA"
                     /locus_tag="ACHUT9_003338"
     CDS             <1..741
                     /gene="hdhA"
                     /locus_tag="ACHUT9_003338"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFR1349742.1"
                     /translation="DGKCAIITGAGAGIGKEIAITFATAGASVVVSDINADAANHVVD
                     EIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPKPFDMPMADF
                     RRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYASSKAAASHL
                     VRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRLGQPQDIANA
                     ALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            962..1552
                     /gene="uidR"
                     /locus_tag="ACHUT9_003339"
     CDS             962..1552
                     /gene="uidR"
                     /locus_tag="ACHUT9_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFR1349743.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1941..3752
                     /gene="uidA"
                     /locus_tag="ACHUT9_003340"
     CDS             1941..3752
                     /gene="uidA"
                     /locus_tag="ACHUT9_003340"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFR1349744.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3749..5122
                     /gene="uidB"
                     /locus_tag="ACHUT9_003341"
     CDS             3749..5122
                     /gene="uidB"
                     /locus_tag="ACHUT9_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFR1349745.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5161..6471
                     /gene="uidC"
                     /locus_tag="ACHUT9_003342"
     CDS             5161..6471
                     /gene="uidC"
                     /locus_tag="ACHUT9_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFR1349746.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(6652..8160)
                     /locus_tag="ACHUT9_003343"
     CDS             complement(6652..8160)
                     /locus_tag="ACHUT9_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFR1349747.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHUT9_003344"
     CDS             complement(8261..9436)
                     /gene="manA"
                     /locus_tag="ACHUT9_003344"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFR1349748.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9635..11281
                     /gene="fumB"
                     /locus_tag="ACHUT9_003345"
     CDS             9635..11281
                     /gene="fumB"
                     /locus_tag="ACHUT9_003345"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFR1349749.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2812 a         2650 c         2916 g         2917 t
ORIGIN      
        1 gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt
       61 acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga cgcagctaac
      121 catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg ttgtgatatt
      181 acttccgaac aggaactctc tgcactggca gactttgcta tcagtaagct gggtaaagtt
      241 gatattctgg ttaacaacgc cggtggcggt ggacctaaac cgtttgatat gccaatggcg
      301 gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc acaacttgtt
      361 gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc tatggcggca
      421 gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcagc cagtcatctg
      481 gtcagaaata tggcgtttga cctgggtgaa aaaaatattc gggtaaatgg cattgcgccg
      541 ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga acaaaaaatg
      601 ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa cgcagcgctg
      661 ttcctttgct cgcctgctgc gagctgggta agcggacaaa ttctcaccgt ctccggtggt
      721 ggggtacagg agctcaatta atacactaac ggaccggtaa acaaccgtgc gtgttgttta
      781 ccgggataaa ctcatcaacg tctctgctaa ataactggca gccaaatcac ggctattggt
      841 taaccaattt cagagtgaaa agtatacgaa tagagtgtgc cttcgcacta ttcaacagca
      901 atgataggcg ctcacctgac aacgcggtaa actagttatt cacgctaact ataatggttt
      961 aatgatggat aacatgcaga ctgaagcaca accgacacgg acccggatcc tcaatgctgc
     1021 cagagagatt ttttcagaaa atggatttca cagtgcctcg atgaaagcca tctgtaaatc
     1081 ttgcgccatt agtcccggga cgctctatca ccatttcatc tccaaagaag ccttgattca
     1141 ggcgattatc ttacaggacc aggagagggc gctggctcgt ttccgggaac cgattaaagg
     1201 gattcatttt gttgactata tggtcgagtc tattgtctct ctcacccatg aagcctttgg
     1261 gcaacgggcg ctggtggtcg aaattatggc ggaagggatg cgtaacccac aggtcgccgc
     1321 catgcttaaa aataagcata tgacgatcac ggaatttgtt gcccagcgga tgcgtgatgc
     1381 ccagcaaaaa ggcgagataa gcccagacat caacacgtca atgacttcac gtttactgct
     1441 ggatctgacc tacggtgtac tggccgatat cgaagcggaa gatctggcgc gtgaagcgtc
     1501 gtttgctcag ggattacgcg cgatgattgg cggtatctta accgcatcct gatcctctca
     1561 tttcgcgggc tggtgataac tgtgcccgcg tttcatactt taatttccct gtacaaaata
     1621 caatctagcc gggatcggag aattcccccc caaaaatatt caccgtagcc atatgtcatg
     1681 agagtttatc gttcccaata tgctcgaacg aacgttcggt tgcttatttt atggcttctg
     1741 tcaacgccgt tttaaagatt aatgcgatca atatcacgct gtgggtattg cagtttttgg
     1801 ttttttgatc gcggtgtcag ttctttttat ttccatttct cttccatggg tttctcacag
     1861 ataactgtgt gcaacacaga attggttaac taatcagatt aaaggttgac cagtattttt
     1921 atcttaatga ggagtccctt atgttacgtc ctgtagaaac cccaacccgt gaaatcaaaa
     1981 aactcgacgg cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt
     2041 ggtgggaaag cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc
     2101 agttcgccga tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct
     2161 ttataccgaa aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt
     2221 acggcaaagt gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat
     2281 ttgaagccga tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg
     2341 tgaacaacga actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg
     2401 gcaagaaaaa gcagtcttac ttccatgatt tctttaacta tgccgggatc catcgcagcg
     2461 taatgctcta caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg
     2521 cgcaagactg taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg
     2581 ttgaactgcg tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt
     2641 tgcaagtggt gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg
     2701 tcacagccaa aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag
     2761 tggcagtgaa gggcgaacag ttcctgatta accacaaacc gttctacttt actggctttg
     2821 gtcgtcatga agatgcggac ttgcgtggca aaggattcga taacgtgctg atggtgcacg
     2881 accacgcatt aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg
     2941 ctgaagagat gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg
     3001 ctgtcggctt taacctctct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac
     3061 tgtacagcga agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag
     3121 agctgatagc gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac
     3181 cggatacccg tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta
     3241 aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     3301 ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     3361 aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     3421 agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     3481 actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     3541 accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     3601 ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaggggatc ttcacccgcg
     3661 accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     3721 gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     3781 cggctacagc ctcggtgacg tcgccaataa cttcgccttc gcaatggggg tgctcttcct
     3841 gttgagttac tacaccgacg tcgctggcgt cggtgccgct gcggcgggca ccatgctgtt
     3901 actggtgcgg gtattcgatg ccttcgccga cgtctttgcc ggacgagtgg tggacagtgt
     3961 gaatacccgc tggggaaaat tccgcccgtt tttactcttc ggtactgcgc cgttaatgat
     4021 cttcagcgtg ctggtattct gggtaccgac cgactggagc catagccgca aagtagtgta
     4081 tgcatatttg acctacatgg gcctcgggct ttgctacagc ctggtgaata ttccttatgg
     4141 ttcacttgct accgcgatga cccaacaacc acaatcccgc gcccgtctgg gcgcggctcg
     4201 tgggattgcc gcttcattga cctttgtctg cctggcattt ctgatagggc cgagcattaa
     4261 gaactccagc ccggaagaga tggtgtcggt ataccatttc tggacgattg tgctggcgat
     4321 tgccggaatg gtgctttact tcatctgctt caaatcgacg cgtgagaatg tggtacgtat
     4381 cgttgcgcag ccgtcattga agatcagttt gcaaaccctg aaacggaatc gcccgctgtt
     4441 tatgttgtgc atcggtgcgc tgtgtgtgct gatttcgacc tttgcggtca gcgcctcgtc
     4501 gttgttctac gtgcgctatg tgttaaatga taccgggctg ttcactgtgc tggtactggt
     4561 gcaaaacctg gttggtactg tggcatcggc accgcttgtg ccggggatgg tcgcgaggat
     4621 cggtaaaaag aataccttcc tgattggcgc tttgctggga acctgcggtt atctgctatt
     4681 cttctgggtt tccgtctggt cactgccggt ggcgttggtt gcgttggcca tcgcttcaat
     4741 tggtcagggc gttactatga cagtgatgtg ggcactggaa gctgataccg tagaatacgg
     4801 tgaatacctg accggcgtgc gaattgaagg gctcacctat tcactattct catttacccg
     4861 taaatgcggt caggcaatcg gaggttcaat tcctgccttt attttggggt taagcggata
     4921 tattgccaat caggtgcaaa cgccggaagt tattatgggc atccgcacat caattgcctt
     4981 agtaccttgc ggatttatgc tactggcatt cgttattatc tggttttatc cgcttacgga
     5041 taaaaaattc aaagaaatcg tggttgaaat tgataatcgt aaaaaagtgc agcagcaatt
     5101 aatcagcgat atcactaatt aataatcaat aaaaataatc agaacatcaa aggtgcaact
     5161 atgagaaaaa tagtggccat ggccgttatt tgcctgacgg ctgcctctgg ccttatctct
     5221 gcttatgcgg cgcaactggc tgacgatgag gcgggactac gcatcagact gaaaaacgaa
     5281 ttgcgcaggg cggataagcc cagtgctggc gcgggaagag atatttacgc atgggtacag
     5341 ggaggattgc tcgatttcaa tagtggttat tattccaata ttattggcgt tgaaggcggg
     5401 gcatattatg tttataaatt aggtgctcgt gctgatatga gtacccggtg gtatcttgat
     5461 ggtgataaaa gttttggctt tgccctaggg gcagtaaaaa taaaacccag tgaaaatagc
     5521 ctgcttaaat caggtcgctt cgggacggat tatagttatg gtagcttacc ttatcgtatt
     5581 ccgttaatgg ctggcagttc gcaacgtaca ttaccgacag tttctgaagg agcattaggt
     5641 tattgggctt taacaccaaa tattgatctg tggggaatgt ggcgttcacg agtattttta
     5701 tggactgatt caacaaccgg tattcgtgat gaaggggtgt ataacagcca gacgggaaaa
     5761 tacgataaac atcgcgcacg ttctttttta gccgccagtt ggcatgatga taccagtcgc
     5821 tattctctgg gggcatcggt acagaaagat gtttccaatc agatacaaag tattctcgag
     5881 aaaagcatac cgctcgaccc gaattatacg ttgaaagggg agttgctcgg cttttacgcg
     5941 cagctcgaag gtttaagtcg taataccagc cagcccaatg aaacggcgtt ggttagtgga
     6001 caattgacct ggaatgcgcc gtggggaagc gtatttggca gtggtggtta tttgcgccat
     6061 gcaatgaatg gtgccgtggt ggataccgac attggctatc ccttttcatt aagtcttgat
     6121 cgtaaccgtg aaggaatgca gtcctggcaa ttgggcgtca actatcgttt aacgccgcaa
     6181 tttacactga catttgcaca gattgtgact cgcggctatg aatccagtaa acgagatgtg
     6241 cggattgaag gcgcgggtat cttaggtggt atgaactatc gggtcagcga agggccgtta
     6301 caagggatga atttctttct tgctgccgat aaagggcggg aaaagcgcga tggcagtacg
     6361 ctgggcgatc gcctgaatta ctgggatgtg aaaatgagta ttcagtatga ctttatgctg
     6421 aagtcagaaa taacgccgga gagaaaaacc tccggcgttt cagattgttg acaaagtgcg
     6481 cgttttttat gccggatgcg gcgtaaacgc cttatccagc ctacaaaaac tcataaattc
     6541 aaagtgttgc aggaaaaggt aggcctgata agcgtagcgc atcaggcaat ctctggtttg
     6601 ttttcagatg aaaacgccga agtgaaaatt ctccggcgtt ttggccgtga attactgctg
     6661 cggaattgcc ggtacagccg gaacgttaag agctggcatc gcaaacatac caacgaaatc
     6721 ttccagtggc attttctgcc cgtttaacgt tatctgaccg ttagtatatt gcaggctggt
     6781 ggtgatggta ttgtcctgca aggtggtcag acggaacatc tgccccattg ctgatgcacc
     6841 ttcaacttgc tgtttcgcca gttttttcgc ttgatcttcc tgataacctt ccagcttcgc
     6901 tacctgagtc ataaactcag ttgccatatc caccggaatg gtcagtttcg catccagaga
     6961 tttaaccgaa cgatctactt cctgcgccag cgtttgcggc gcttctttag tcgttgccgg
     7021 atctttcagg aacagcgaca gattcagcgc actttcaccc tgactgtttt tccagcttag
     7081 cggcgcgata gtaatcaccg gatcgccttt caacatcagc ggcagggcgc taaagaaggc
     7141 ttccgtcact ttctcctgat aaagttcggg attgttggca atttctggct gtgccagcag
     7201 cgcctgagtt tgcgcgttat attgctggct aaactgatgc caggcttcac catcaatctg
     7261 gccgactttt aaagtcagct tgccgctgcc cagatcctga ttctgtacct tcaggctgtt
     7321 tagcgagtaa tccagttggc tattgatcgt tttaccgtca ttgaccagat ccgatttacc
     7381 gctgatctcc atgccttcca gcagtgccag ttctttgcct tccactgaaa tggtcatttt
     7441 ttccagtgac agtttttggt ttcctacgcg ctcaccaaaa cttgccagcg tgctggaacc
     7501 gtcggttttc agattattaa aggtcaactg cactttctgg ttgtattcgt taactgcgtc
     7561 tatccgacca ctttgcgcct ccccggaaag ggagatggct ttgccgtctc tgtcagcatt
     7621 taactggaac tcgccgccgc taaaggcgac tttttcatcc ttttgctcgt aattcagtgg
     7681 cttgagcgaa atatcggaac tggaatcacc gctgtaacca atgcgcgagt taatctcaaa
     7741 aggcgtttca ccttttgcca tatcaaacag tggtttgctt acttcgttat taaccagcgt
     7801 ggtttgaatt gatgccatcg acgggatcag gttcagtttt ttaagctggg caagcgggaa
     7861 gggaccatga tcaaccgatt cgttgaagat gacgctctga ccgcttttaa tccacggatt
     7921 ttctttcccg gcaatgggtt tcaccaacag ttgcaactgg ctgctgaata cgccgcgatg
     7981 atagttttga taactcactt ccaggttgga ttcaggagct gtcagtttga gttgcgcgtt
     8041 cgcctgcgcg accatgtctt cgagatgggt ttcaatcttc ttgcctgtat accatgcgcc
     8101 gcctgtccag actacgccta gcgcaacaat gacgcctacc gctaccagcg atttattcat
     8161 aatgattatc cataaaatga aatcaggcgg actggccgcc tgaaggtgtt ataagccttt
     8221 aataagctta gcaagagatg ttaatttttt cagtaagctc ttacagcttg ttgtaaacac
     8281 gcgctaaacg gccgtggcct ttgacagtca ccggtgattc gttggcggca ataaacgctg
     8341 attcacccgg tttaagctgt aactgctgag aacctttcca caacgttgca tcgccttcga
     8401 cgcagaacaa aatggcggca ctctgctggc taatggtggt ttctttatca ctaaggtcat
     8461 gcagcgagaa ggcaaaatca tccactggaa tcgggaagtc cagttctgca ccttgtttca
     8521 ccggctgggt caacaactgg ttagccggtt tggcttcgaa tttcacattg gcaaccagtt
     8581 ccggaatatc aatgtactta ggcgtcagac ccgcacgcag cacgttatcg gagttcgcca
     8641 tcacttccag cgccacgcct tgcaggtaag cgtgcggtgt ttcagcgaac aggaacatcg
     8701 cttcgccagg attcaatttc accacattca gtaacagcgg ggagaacagg ccactgtctt
     8761 ccgggtaaaa ttcagaaatt aaacgaatcg tttgccacgg ttcaccctgc tgactgtcga
     8821 gcgccgattt taaaatcgcc agcgcgcggg atttttcttc accctgcata ttcaacaggc
     8881 tggcgaacag ttcgcttaaa cgttcggcat taggctgttg taaaaagtga gcaattgccg
     8941 gatgtgcacc tgcgactggc tggagtaggg agacaatctc ggaaaactca cgaaacgcgt
     9001 tcatcgcaag gaaaggcgtc agcgcaaaaa ccagctccgg cttgtggtta ggatctttat
     9061 agttacgctc ggcggcatcc atcgggatac ctgcggcatt ttctttggca aaaccgattt
     9121 cagaattgcg tttgtttgga tgaacctgaa tggagagcgg ctgtgctgcg cataatactt
     9181 tgaacaggaa aggcagttcg ccaaagcgtt tggcaacggc ctctccgagc agagtcgatt
     9241 tatcactctc aatcacatca cgcagtgaaa cgatatctcc ggcggcattc tgcactcgtg
     9301 aactgctttt cggatgtgcg cccatccaca gctcggccat cggctggctg gacggatttt
     9361 ccataccata aagttcagtc aacgccgttt tgctgcccca ggcatagttt tgcactgagt
     9421 taatgagttt ttgcatgatc aatccctgtt ttaatgtgga aattaatccc actattaaag
     9481 caagaatcct acgggaagta acctggagcc gcaaaaagtc gtactagtct cagtttttgt
     9541 taaaaaagtg tgtaggatat tgttactcgc ttttaacagg gcaacggaac acccgcccag
     9601 agcataacca aaccaggcag taagtgagag aacaatgtca aacaaaccct ttcattatca
     9661 ggctcctttt ccactcaaaa aagatgatac tgagtattac ctgctaacca gcgaacacgt
     9721 tagcgtatct gaatttgaag ggcaggagat tttgaaagtc gcacccgaag cgttaactct
     9781 gttggcgcgt caggcgtttc atgatgcgtc atttatgctg cgtccggctc accaacaaca
     9841 ggtggccgac attctgcgtg acccggaagc cagcgaaaat gataaatatg tggcgctgca
     9901 attcctgcgc aactccgaca tcgcggcgaa aggtgttctg ccaacctgtc aggataccgg
     9961 cactgcgatt attgttggta aaaaagggca gcgtgtatgg accggtggct gtgatgaagc
    10021 ggcgctggcg cgcggtgtct ataacactta tatcgaagat aatttgcgct actcgcaaaa
    10081 cgcgccgctg gatatgtata aagaggtgaa taccggcacc aatctgccag cgcagatcga
    10141 tctttatgcc gttgatggcg acgagtacaa attcctctgt atcgccaaag gtggtggttc
    10201 ggcaaacaag acgtatctct atcaggaaac caaagcgtta ctaacgccgg ggaaactgaa
    10261 aaattacctg gttgagaaga tgcgcacgct gggtacggcg gcctgtcctc cgtatcatat
    10321 tgcgttcgta attggtggaa cttctgcaga aactaacctt aaaacagtga aactggcttc
    10381 cgctaaatac tatgacgaac tgccaacgga agggaatgag cacggtcagg cattccgcga
    10441 tgtggaactg gaaaaagaat tgctgatcga agcgcaaaat ctcggtctgg gtgcgcagtt
    10501 tggtggtaaa tacttcgctc acgacatacg cgtgattcgc ctgccacgtc acggcgcatc
    10561 ctgtccggtt ggtatgggtg tttcctgctc tgctgaccgt aatatcaaag cgaagatcaa
    10621 ccgtcagggg atctggatcg aaaaactgga acataatcca ggtaaatata tccctgaaga
    10681 gctgcgtaaa gcgggagaag gcgaagcggt gcgcgttgac cttaaccgtc cgatgaaaga
    10741 gatcctcgca cagttgtcgc agtatcccgt ttctacacgc ttatcgctta acggcacgat
    10801 tatcgtcggt cgtgatattg ctcacgccaa actgaaagag cggatggata acggtgaagg
    10861 gctgccgcag tacattaaag atcatccgat ttactacgcg ggtccggcca aaacgccgga
    10921 aggttatgcc tccggttctc ttggcccaac gaccgccgga cggatggatt cttatgtcga
    10981 tcaactgcaa gcgcagggcg gaagtatgat catgctggcg aaaggcaacc gcagccagca
    11041 ggtgacggat gcctgtaaaa aacacggcgg cttctacctt ggcagtatcg gtggtccggc
    11101 cgctgtattg gcgcagggaa gtattaagag cctggaatgt gttgaatatc cggaactggg
    11161 aatggaagcc atctggaaaa ttgaagtgga agatttcccg gcgtttatcc ttgtggatga
    11221 taaaggaaat gacttcttcc agcagataca actcacacag tgcactcgct gtgtgaaata
    11281 aacagagccg ccctt
//
LOCUS       DAZPEP010000135        11246 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZPEP010000135 DAZPEP010000000
VERSION     DAZPEP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.135"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1760
                     /locus_tag="ACHUT9_003346"
     CDS             <1..1760
                     /locus_tag="ACHUT9_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HFR1349750.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     GRSALLLGKTGEPPNYLYL"
     gene            2096..3073
                     /gene="glaH"
                     /locus_tag="ACHUT9_003347"
     CDS             2096..3073
                     /gene="glaH"
                     /locus_tag="ACHUT9_003347"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFR1349751.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHUT9_003348"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHUT9_003348"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFR1349752.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="ACHUT9_003349"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="ACHUT9_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFR1349753.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="ACHUT9_003350"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="ACHUT9_003350"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFR1349754.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            7437..8837
                     /gene="gabP"
                     /locus_tag="ACHUT9_003351"
     CDS             7437..8837
                     /gene="gabP"
                     /locus_tag="ACHUT9_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFR1349755.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8858..9520
                     /gene="csiR"
                     /locus_tag="ACHUT9_003352"
     CDS             8858..9520
                     /gene="csiR"
                     /locus_tag="ACHUT9_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFR1349756.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHUT9_003353"
     CDS             complement(9521..9970)
                     /gene="kbp"
                     /locus_tag="ACHUT9_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFR1349757.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(10054..10212)
                     /locus_tag="ACHUT9_003354"
     CDS             complement(10054..10212)
                     /locus_tag="ACHUT9_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFR1349758.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10395..10694
                     /locus_tag="ACHUT9_003355"
     CDS             10395..10694
                     /locus_tag="ACHUT9_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFR1349759.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHUT9_003356"
     CDS             10704..11228
                     /gene="ygaP"
                     /locus_tag="ACHUT9_003356"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFR1349760.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
BASE COUNT         2786 a         2785 c         2939 g         2736 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 cgaactatct ctatttataa tttaatgtta tatctgcccc gataaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcag tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctaatgtt aactccccct aacctgttgc tttagttatt catttcctgt
     1921 ctcactttgc cttaatactc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tctcatcccg tcggcgcaat ccccgcgtct gctggaactc accttcaccg aacagacgac
     2221 caaacagttt ctcgagcagg ttgccgaatg gcccgtacag gcgctggagt acaaatcgtt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tgtcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctgt tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgccactc accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ttatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggca gcctgaaggc gcagttttgc ctggcgggga
     3301 accgcgccac caaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctcag ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gagtggataa acgccgtgga actgcgcgag cgcgaaccga
     3481 atatcaccgg gcttggtggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tcacagcggc aatggcaaaa atcttccagg ccagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc cctcagtgag cataaaaacg gcgtggtgat acgcacccgt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggcct cgaaccgggc tttatcatct gcccgttccg tggtgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc cattcccgac cccgcgatgc
     3841 catttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agtgacacgc
     3961 tggagatttt gagctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctacct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcag
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccacccca cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcgg gatcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggc
     4681 aaaccactgg ccgaagcgaa aggcgaaatc agctacgctg cctcttttat tgagtggttt
     4741 gccgaagaag gcaaacgtat ttatggcgat accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgcagcta tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     4921 aagcccgcca gtcagacacc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtgtt taacgtagtc accggttcgg caggcgcggt cggtaatgaa
     5041 ctgaccagca acccgctggt acgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga cgatgccgat ctcgataaag ccgtggaagg cgcgctggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt gccggccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cgctgttccg ctttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcattatttc caatgaagtg gccccgttcg gcggcatcaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggcg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacctgc atccgaaggt ggtggccgca gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ttacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccgg gcgatttcgc caagaaaacg ctgctggtta cgaccggttc
     6181 cgaagcggtg gaaaacgcgg taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gccgcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg tggcgccgga tcttaccacc tttgcgaaat cgatcgcagg
     6661 cggtttcccg ctggcgggcg tcaccgggcg cgcggaagtg atggacgctg ttgcaccggg
     6721 cggtctgggc ggcacctatg ccggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttc gagcatgaaa atctgctgca aaaagccaac gatctggggc agaagctaaa
     6841 agacggattg ctggcgatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 agcgatgatt gccatcgagc tgtttgaaga tggcgatcac agcaaaccgg acgcaaaact
     6961 caccgccgag atcgtggcac gcgcccgcga taagggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttgc tgaggcgaag cagtagctat gtccgtttgt
     7141 aagagttaac cgttgctaag ggaatgataa cgatgcagta aaaaataata acgatgaggc
     7201 gaacgacaaa gttaaaaacg actgcggaca gtcccctcgc ccctctggag agagggttag
     7261 ggtgaggggt aaaacacgcg gctaaaccct cccctggccc tctccctaag ggagagggag
     7321 aaaaccctcc cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc
     7381 ggcgcgggaa atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg
     7441 ggcaatcatc gcaaccacat gagttaggcg gcgggctgaa atcacgccac gtcaccatgt
     7501 tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg
     7561 aagcgggccc ggcggtatta ctggcctatc tgttcgccgg gctactggtg gttatgatta
     7621 tgcggatgtt ggcggaaatg gcggttgcca cgcccgatac cggttcgttt tccacctatg
     7681 ccgataaagc cattggtcgc tgggcgggct ataccatcgg ctggttgtac tggtggtttt
     7741 gggtactggt tatcccgctg gaagccaata tcgccgctat gatcctgcac tcgtgggttc
     7801 caggcattcc catctggtta ttttccctcg tcattaccct tgccttaact ggcagtaatt
     7861 tattaagcgt taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg
     7921 ctatcctggc ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg
     7981 aagtgagcgg gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg
     8041 cggtattaag cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca
     8101 ttgccgccgc ggaatccgac acgccggaaa aacatattgt ccgcgccacc aactcggtta
     8161 tctggcgtat ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaataccgt
     8221 ggaatatgcc ggggctgaaa gccgttggat cttatcgctc ggttctggaa ttgctcaata
     8281 ttccccatgc gaaattaatc atggactgtg tgatattact ttccgtaacc agttgcctga
     8341 actcggccct gtataccgcc tcaaggatgc tctactcctt aagccgtcgc ggtgatgctc
     8401 ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta cgtggcggtg ttactctcca
     8461 ccggcgcggc attcttaacg gtggtggtga actattacgc gcctgcgaag gtatttaaat
     8521 ttctgatcga cagctccggc gctatcgccc tgctggttta tttagtcatc gccgtttcac
     8581 agttgcggat gcgcaaaatt ctgcgagcgg aaggaagcga aattcgcttg cgaatgtggc
     8641 tttatccctg gctcacctgg ctggtcatcg gctttattac ctttgtgttg gtagtgatgc
     8701 tattccgtcc ggcgcaacag ttagaagtga tctccaccgg cttattagcg atagggatta
     8761 tctgtaccgt gccaattatg gctcgctgga aaaagctggt attgtggcaa aaaacacccg
     8821 ttcataatac gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg
     8881 ctatcgctgg ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg
     8941 gatgagtttg ctgacatcgc gttatgcact tggcgttggg ccgttgcggg aagcactttc
     9001 tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc
     9061 tatgtcagag caggagctgc tcgatatttt cgatgcccgc gccaatatgg aagcgatgtt
     9121 agtgagtctg gcgattgccc gcggtggcga tgagtgggag gcagacgttc tcgcaaaagc
     9181 gcatctgctc agtaagctgg aagcctgtga cgccagcgag aaaatgcttg atgagtggga
     9241 tctgcgtcat caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct
     9301 gcaaatgcgt gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg
     9361 aacggtgctt tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc
     9421 ggcggtactg gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac
     9481 gccaattccc attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca
     9541 acacttgccc cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga
     9601 ttttattgta cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc
     9661 cagacttaac ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat
     9721 caacactggc aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct
     9781 gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat
     9841 cagcatcagg tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt
     9901 tatcgtgctg acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga
     9961 acagacccat agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg
    10021 cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc
    10081 gtgaatcaaa ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca
    10141 accgaaccct ttaccgagca gcacgccgag cggcggtaga atgatggtga tgacgattct
    10201 ccagaaaccc atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat
    10261 ctcttaagcg tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat
    10321 ttacgctgct gaggctggta tatgttgcat ttttagttta gagcttactt aatttagaaa
    10381 atacttaaat aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg
    10441 ccttattgaa agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt
    10501 cccccggcac cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat
    10561 cacagcatct cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac
    10621 gcattctata ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg
    10681 tctattgtcc gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag
    10741 aattaatcgc acgcggcgca aagttaatcg atattcgtga cgctgatgaa tatcttcgtg
    10801 aacatattcc tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac
    10861 ctaaattacg tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata
    10921 acgccgataa attagcagcg attgccgccc ccgcagaaat atttttactc gaagatggga
    10981 ttgatggctg gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt
    11041 taatgcgcca ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt
    11101 ataccgtaaa tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg
    11161 caggaatcag cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac
    11221 gagcttgaga agcgacgccg gacgcg
//
LOCUS       DAZPEP010000136        11241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZPEP010000136 DAZPEP010000000
VERSION     DAZPEP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.136"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            3..308
                     /locus_tag="ACHUT9_003357"
     CDS             3..308
                     /locus_tag="ACHUT9_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFR1349761.1"
                     /translation="MLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="ACHUT9_003358"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="ACHUT9_003358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFR1349762.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUT9_003359"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="ACHUT9_003359"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFR1349763.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUT9_003360"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="ACHUT9_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFR1349764.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUT9_003361"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="ACHUT9_003361"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFR1349765.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTLLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUT9_003362"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="ACHUT9_003362"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFR1349766.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUT9_003363"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="ACHUT9_003363"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFR1349767.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUT9_003364"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="ACHUT9_003364"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFR1349768.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUT9_003365"
     CDS             complement(8116..9762)
                     /gene="ilvG"
                     /locus_tag="ACHUT9_003365"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFR1349769.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUT9_003366"
     CDS             complement(9765..9815)
                     /gene="ilvX"
                     /locus_tag="ACHUT9_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFR1349770.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUT9_003367"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="ACHUT9_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFR1349771.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..>11241
                     /locus_tag="ACHUT9_003368"
     CDS             10353..>11241
                     /locus_tag="ACHUT9_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFR1349772.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGRHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNG"
BASE COUNT         2554 a         3090 c         2950 g         2647 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cagcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtgg aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg cccccgatca ctgttgaggt acatatcagt aaaggtctac
    10441 ccggcttaac gatggtgggc ttaccagaaa caactgtaaa agaagctcgc gatcgcgtgc
    10501 gcagcgccat tatcaatagc ggatatgaat atccggcgaa aaaaatcacc atcaaccttg
    10561 ctccagccga tctgccaaaa gaagggggac gatatgattt acctatcgcc attgcgttgc
    10621 tggcggcctc agaacagctt acagccaata agttagatga atatgaatta gtcggagaac
    10681 tggctcttac aggcgctctg cgtggcgttc ccggtgcaat ctccagtgca actgaagcta
    10741 ttaagtcggg cagaaaaatt atcgtcgcga aagataacga agatgaagtg gggctaatta
    10801 acggtgaagg atgcctgata gccgatcatc tgcaagctgt ctgtgcgttt ctggaaggta
    10861 agcacgctct cgaacgcccg aaaccaactg atgcagtatc ccgggcgcta caacatgatc
    10921 tcagtgatgt tgtcggtcag gaacaaggaa agcgaggact ggaaattacc gccgctggca
    10981 ggcacaacct tttactgatt gggccgccgg gaacaggtaa aacaatgctc gccagccgta
    11041 ttaatggcct gttgccagat ttaagcaatg aagaggcact ggagagcgct gcgatattaa
    11101 gtctggtaaa tgctgaatca gtacaaaaac aatggcggca gcgcccgttc cgctcacctc
    11161 atcacagtgc gtcgttaact gcgatggtgg gcggtggtgc aattccaggg ccaggtgaaa
    11221 tttcgttggc gcataacggc g
//
LOCUS       DAZPEP010000137        11169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZPEP010000137 DAZPEP010000000
VERSION     DAZPEP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.137"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            45..120
                     /locus_tag="ACHUT9_003369"
     tRNA            45..120
                     /locus_tag="ACHUT9_003369"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:78..80,aa:Lys,seq:ttt)
     gene            151..276
                     /locus_tag="ACHUT9_003370"
     ncRNA           151..276
                     /ncRNA_class="other"
                     /locus_tag="ACHUT9_003370"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            396..737
                     /locus_tag="ACHUT9_003371"
     CDS             396..737
                     /locus_tag="ACHUT9_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFR1349773.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(728..1654)
                     /locus_tag="ACHUT9_003372"
     CDS             complement(728..1654)
                     /locus_tag="ACHUT9_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1349774.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGWEGAPLYQI"
     gene            1744..2742
                     /locus_tag="ACHUT9_003373"
     CDS             1744..2742
                     /locus_tag="ACHUT9_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFR1349775.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(2739..2957)
                     /locus_tag="ACHUT9_003374"
     CDS             complement(2739..2957)
                     /locus_tag="ACHUT9_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFR1349776.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(2959..4974)
                     /gene="ligA"
                     /locus_tag="ACHUT9_003375"
     CDS             complement(2959..4974)
                     /gene="ligA"
                     /locus_tag="ACHUT9_003375"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFR1349777.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(5045..6031)
                     /gene="zipA"
                     /locus_tag="ACHUT9_003376"
     CDS             complement(5045..6031)
                     /gene="zipA"
                     /locus_tag="ACHUT9_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFR1349778.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            6261..7022
                     /gene="cysZ"
                     /locus_tag="ACHUT9_003377"
     CDS             6261..7022
                     /gene="cysZ"
                     /locus_tag="ACHUT9_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFR1349779.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            7207..8178
                     /gene="cysK"
                     /locus_tag="ACHUT9_003378"
     CDS             7207..8178
                     /gene="cysK"
                     /locus_tag="ACHUT9_003378"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFR1349780.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            8562..8819
                     /gene="ptsH"
                     /locus_tag="ACHUT9_003379"
     CDS             8562..8819
                     /gene="ptsH"
                     /locus_tag="ACHUT9_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFR1349781.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            8864..10591
                     /gene="ptsI"
                     /locus_tag="ACHUT9_003380"
     CDS             8864..10591
                     /gene="ptsI"
                     /locus_tag="ACHUT9_003380"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFR1349782.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            10632..11141
                     /gene="crr"
                     /locus_tag="ACHUT9_003381"
     CDS             10632..11141
                     /gene="crr"
                     /locus_tag="ACHUT9_003381"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFR1349783.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         2719 a         2883 c         2777 g         2790 t
ORIGIN      
        1 aaggaggggg tcggcggttc gatcccgtca tcacccacca cttcgggtcg ttagctcagt
       61 tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca
      121 atgtaaaaaa gcgccctaaa ggcgcttttt tgctatctgc gatactcaaa gattcgaacc
      181 tgcagcaggt ttgagttgag cgcagcgaaa caacggagcc gctcgcggcg acggcccgaa
      241 gggcgagcga agcgagtcat cctgcacgac ccaccaatgt aaaaaagcgc cctaaaggcg
      301 cttttttgct atctgcgatt tgtaagattg atttttcttc tcaagttcct tgttcttctg
      361 ccgatattac ctcgtacagt gacgaggact gaacaatggc cactatctct tcaacttcaa
      421 ttccttccat acaaacacag agtagtaaca gggcatcaca aggtagcgat gttgcttcaa
      481 aaatcgcgcg gataagtcag caaatcatca aactcactca acaaattaaa gagattgtag
      541 acactagcgg cagtgctgaa gacaaacaaa aacaggccga attgattcaa cagcaaatca
      601 ccctactgga aacacaactg gcgcagttgc agagacagca ggcagaaaag gcgcaggaga
      661 aagagcagcg tttgtctctg aatgtatccc ttctaaatcc tgttgaaaat acgactcata
      721 tcgatatcta tatctgatac aacggagccc cttcccaacc agccattagc gcattgccct
      781 ggtctcgtac cacgtcccat aacgcttcag cggcggtgga aagtgaacgg ttcttcctgc
      841 gcaccagcat caactggcgt tcaacaaccg gcgtaatgcg ctttaccacc agtgggctac
      901 cttcaggcaa tggtagcgcc agtgcgggaa gaatactgat gccgatgcct gcggctacca
      961 tcgggaacag cgtcgccgga tgtccaatct cctgcacaat attcgcctga ataccattgc
     1021 gcgccagcgc tgcgtcaatc agcggtcggc tgcctgacgc gtaatcctgc aacaccaatt
     1081 tcgctccctg taatgcctgc cagggtacat aatcttccac ggccaaagcg ctatcgcgat
     1141 ggcaaagcag aaagaaaggc tcggaaagaa tcgcttcaca ttgcagatcg cccactgggc
     1201 ctggatcgat gacgatgcaa aaatcgacat ctccctggcg aatactttcc atcacccact
     1261 gctgcggacg atcgtgcagg acaaactgaa tatctggata gcggcgatgg ctttcagcaa
     1321 tgcattgcgg aataagatgc gcggaaatgg tctggctggc agcgacccga acttttccgc
     1381 tcagttgttg ccccatacgc ccggtatcgc gcaacgtgct gttaagttca tccagcagtc
     1441 gctcaagacg caaagccaac tgctgccctg catctgtaag caccacttca cgcgtggttc
     1501 tgtccagcag gcgaacacca gtatgatttt ccagctcctt cacactgtga cttactgccg
     1561 actggctcaa gccgatacgc tctcctgcac gactaaaact tttctcctgc gctactgtga
     1621 cgaaaacctt taattgtttt aaagaataat tcatctgttt tcttcatgaa tggatgcaat
     1681 aaatcaattt tatttctcaa actggaagaa gcacaataga accatcgatc atctggagtc
     1741 tttatgaaac tttttcgtat cctcgatcct ttcaccttaa ccctgatcac ggtggtgttg
     1801 ctggcctctt tctttccggc cagaggcgat ttcgtcccct tctttgaaaa tctgaccacc
     1861 gcagctattg ccctgctgtt ctttatgcac ggcgcgaagt tgtcgcgtga ggcgattatt
     1921 gctggcggtg gtcactggcg actgcatttg tgggtaatgt gcagcacctt cgtgctgttt
     1981 ccgattctgg gtgtactgtt tgcctggtgg aaaccggtaa atgtcgaccc gatgctctac
     2041 tccggttttc tctacttgtg cattctcccg gctaccgtgc agtctgcaat cgccttcacg
     2101 tcaatggcgg gcggtaacgt cgcggcggcg gtttgttctg cgtcagcatc cagcctgctg
     2161 gggattttcc tttcaccatt gctagttggt ctggtgatga atgttcacgg tgcagggggc
     2221 agccttgagc aggtcggtaa aattatgctg caactgctgc tgccgtttgt gttggggcat
     2281 ctttcccggc cgtggattgg tgactgggtg tcgcgcaata aaaaatggat tgcgaaaact
     2341 gaccagacgt ccattctgtt ggtggtttat acagcgttca gcgaagccgt cgttaatggt
     2401 atctggcata aagttggctg gggatcattg ctgtttatcg tggtggtcag ctgcgttctt
     2461 ctggctatcg tgattgtagt taacgtcttt atggcacgcc gactgggctt caataaggca
     2521 gatgaaatta ctatcgtctt ttgtggttcg aaaaagagtc tggcaaatgg catcccgatg
     2581 gcaaacattc tgttccccac atcggtgatc ggtatgatgg tgctgcccct gatgattttc
     2641 catcagatcc aattgatggt ctgtgcggtg ctggcgcgtc gatacaaacg ccagaccgaa
     2701 cagttacagg cgcagcagga aagcagcgcc gataaagctt aaagcggacg cttcaggggc
     2761 tggaccagtt gcgtcagccc ctcggtttta atcagcagcg tgatttgcat tagctcaccg
     2821 agcttccctg ccgggaactc atctttgcgg gcaaaccaca gcagatactc ctccggcagg
     2881 tcgattaagc gacgcccttt gtatttgcca aacggcatta tcgtattggc tatttcaatc
     2941 agctgctctt tttccatctc agctacccag caaacgcagc atttccgctt cgtcgatgac
     3001 ttcaatgccc agttcctgcg ccttcgccag tttagatcct gcagcttcac ccgctatcac
     3061 cagatcggtt ttcttcgaca cgctgcccgc gactttcgcg cccagttcga ccagtcgagc
     3121 tttagcgtca tcacgcgaca tctggcttaa gctgcccgta agcaccacgg ttttaccagc
     3181 aaacgggctg tcaatctctt ccgcgttgat aacgatcggc gcaggccagt gaacaccttc
     3241 cgccaacagc tcgctgatga cattgcggtt gctttcttcg gcaaagaagt tgtgaacgtg
     3301 ggatgcaacg acaatgccaa catcaggcac cttttgcagc tcttcaatcg aagcggcttc
     3361 cagcacttcc agcgtgccga aatatgccgc cagacctgct gcggtggcct cgccgacttc
     3421 acggatgcca agtgcataga ggaagcgagc aaaggtggtt tctttcgctt tttccagcgc
     3481 gttaaccacg ttttgtgccg attttggccc catacgctcc agtccggtca gtttgcctgc
     3541 ggtgagtttg aacagatctg ccggagtgtg gacatattct ttttcaacca gctgatcgat
     3601 gattttgtcg cccattccgt caacatccat cgcacggcgg gaaacaaagt gtttcagcga
     3661 ctctttacgc tgcgcaccgc aaatcaggcc accggtacag cgggcaaccg cttcaccttc
     3721 cacacgctca acgtcagaac cacataccgg acaatgcgtc gggaatacaa cctcacgggt
     3781 atcttccggg cgttcagaaa gcacgacgtt aaccacctgc gggatcacgt cgccagcgcg
     3841 gcgaatcacc actttatcgc caatgcgtaa accaagacgt tcgatttcat ccgcattgtg
     3901 taaggttgcg ttactcacca gcacgccagc aacatggaca ggttccagac gcgcaacagg
     3961 cgtaatcgcg ccagtacgcc caacctgaaa ctcgacgtca cgcacaaagg tcatctgctc
     4021 ctgcgccggg aatttaaacg ctaccgccca gcgcggggca cgcgcgacaa agccaagctg
     4081 ctcctgctgt tccagtgagt tgaccttaat caccacgccg tcgatatcaa agcccagcgt
     4141 cgggcggtct tcttccactt tgtgatagaa cgccagcact tcttccgccg attcacaaag
     4201 cgttacccga tcgctgaccg gcaaccccca ctttttaaat tgcagtaaac ggccaagatg
     4261 agtatccggc agctcgccac cttccagaac accaacgcca tagcagaaaa aagtgagcgg
     4321 tcgcttcgct gtaatacgcg gatcaagctg acgcagtgaa ccagctgccg cattacgtgg
     4381 gttagcaaaa actttcccgc ccgtgcgtcg cgcatcttcg ttaatctttt cgaaccccgc
     4441 ctgcggcagg aacacttcac cacgcacttc cagacgcgcc gggatattct ctccgtgcag
     4501 cttcagcgga atggcgcgaa tagtacgcac attagacgtg atatcttccc cggtggtgcc
     4561 atcgccacgg gtcgcggcac tgactaaaac gccattttca tacagaatac tgacggcaag
     4621 gccatccagc ttcagctcac agcaccaggt gactttctcg ttgtttttca gacggtcctg
     4681 cacacgtttg ttgaaagcaa gaaagctttc ttcatcaaaa acgttatcca gtgacagcat
     4741 tggtacttca tggcgtatct ggctgaaagc cgccagcggc gcagcaccta cacgttgggt
     4801 aggcgaatca ggcgtaatca gttctggatg tttggtttcc agctcgcgca gttcgcgcat
     4861 cagcctgtcg tattcagcgt cgggaatttc cggcgcatcc atcacatgat aaagatattc
     4921 atgatggcga agcgtcgttc gcagttctgt cagttgttgt tcgattgatt ccatatcgca
     4981 ccatcaatgc taaaaacccc cgacaagcgg gggttcgaag aggagttaat ttgccttaag
     5041 tgtatcaggc gttggcgtct ttgacttcgc ggatgatgtc ctggtactcg cgcaatttct
     5101 gcggagtcat catacggcgc tgatcgtcaa gcacgacacc gcccacttca tcggcaatat
     5161 gctgcgcaga ttgcagcatc agcttgaagt tctgcagctc gtcaccgtaa gacggtacct
     5221 gcataaagat agtgacaccc ggagtagtga aatccttcat ttcaggatca aaggttcccg
     5281 gtttcaccat attcgccagg ctgaataacg ccgggccgct gccatccggg ctaagatgac
     5341 gatggtaaat attcatatcg ccaaaaatga agcccgcttg ttgaatgctg ttaagaagca
     5401 gttcaccgtt tagctcgcta ccgtgatgcg ccgcgacgtt catgataatc accgcttctt
     5461 tgcgcttcgg tttatccata actggagccg gttccgctac aggctcaggc tgtggtgccg
     5521 ctacgggttc tgcaggctgg aaagcctgtt gtgccggttg cggtgctgaa tgcacaggct
     5581 gcggcgctgg cgcgacctgt ggcgaaactg gctgctgcaa cggctgttca ggctgcggct
     5641 gataggcagg ctgctgcatc ggctgcgctg gacgcggagc atgttgcggc ggtacctgcg
     5701 cttcaggcgg ctgctggatc ggttgacgcg gctgcgcaga cgcataaggc ggttggtact
     5761 ggtgttgcgg cgacggacga gcagcctcat gctcctgagc gttagccggg gcatgattca
     5821 cgcggtgaac acgaacctca ccaacgccct catcatcttc gacatcctcg tcataagaat
     5881 cgtcgtcacg ttttgacttc attcgtttta atggccgatc gcggaacata gaagatcgtt
     5941 ctttacggct ggtccagaaa ccatgtacca gtaaagcgat tatggcgatc gcgccaacaa
     6001 tgattaatat cagacgcaaa tcctgcatca ttatattctc tgttgttcta acaccttgcc
     6061 accacggcaa acatttactc actaagagta tttgccgatt acctcaagtg caagtgcact
     6121 attaactttc acagcacaaa gatagatgaa atcgtgcttt ttgctgtttt ttcgaacata
     6181 tcctaactgt ccattgcgca attacccggt aaaatacgca gaattttcct gggattggtc
     6241 aaaaggagct catcctgatt atggtttcat cattcacatc tgccccacgc agcggttttt
     6301 actattttgc gcaaggctgg aagctcgtct cgcaacctgg gattcggcgt ttcgttattt
     6361 taccgctgct ggtcaatatt ttgttgatgg ggggcgcatt ctggtggctc tttacacagc
     6421 tcgatgtctg gatcccgact ctcatgagtt acgttccgga ctggctacaa tggctgagtt
     6481 atctgttgtg gcctctggct gtcatctctg tgctgttagt gtttggctat ttcttctcca
     6541 cgattgctaa ctggattgcc gctccgttta acggtttatt ggctgaacaa ctggaagcac
     6601 gattgactgg cgctacaccg ccagataccg ggattttcgg tatcatgaaa gatgtgccgc
     6661 gaatcatgaa acgcgaatgg caaaaatttg cctggtatct gccgcgcgca attgtattac
     6721 taattcttta cttcatccct ggtattgggc aaaccgtcgc gccggtactg tggttcctgt
     6781 ttagcgcctg gatgttagcc atccagtatt gcgattaccc cttcgataac cacaaagtgc
     6841 cgtttaaaga gatgcgcacc gccctgcgca cacgcaaaat caccaatatg cagtttggtg
     6901 ctttaaccag cctgtttacg atgatcccgc tgcttaatct gttcatcatg cccgttgccg
     6961 tttgtggcgc gacggcgatg tgggtcgatt gctatcgcga taaacacgcg atgtggcggt
     7021 aacaatctac cggttatttt gtaaaccgtt tgtgtgaaac aggggtggct tatgccgccc
     7081 cttattccat cttgcatgtc attatttccc ttctgtatat agatatgcta aatccttact
     7141 tccgcatatt ctctgagcgg gtatgctacc tgttgtatcc caatttcata cagttaagga
     7201 caggccatga gtaagatttt tgaagataac tcgctgacta tcggtcacac gccgctggtt
     7261 cgcctgaatc gcatcggtaa cggacgcatt ctggcgaagg tggaatctcg taaccccagc
     7321 ttcagcgtta agtgccgtat cggtgccaac atgatttggg atgccgaaaa gcgcggcgtg
     7381 ctgaaaccag gcgttgaact ggttgaaccg accagcggta ataccgggat tgcactggcc
     7441 tatgtagctg ccgctcgcgg ttacaaactc accctgacca tgccagaaac catgagtatt
     7501 gaacgccgca agctgctgaa agcgttaggt gcaaacctgg tgctgacgga aggtgctaaa
     7561 ggcatgaaag gcgcaatcca aaaagcagaa gaaattgtcg ccagcaatcc agagaaatac
     7621 ctgctgctgc aacaattcag caatccggca aaccctgaaa ttcacgaaaa aaccaccggt
     7681 ccggagatct gggaagatac cgacggtcag gttgatgtat ttattgctgg cgttgggact
     7741 ggcggtacgc tgactggcgt cagccgctac attaaaggca ccaaaggcaa gaccgatctt
     7801 atctctgtcg ccgttgagcc aaccgattct ccagttatcg cccaggcgct ggcaggtgaa
     7861 gagattaaac ctggcccgca taaaattcag ggtattggcg ctggttttat cccggctaac
     7921 ctcgatctca agctggtcga taaagtcatt ggcatcacca atgaagaagc catttctacc
     7981 gcccgtcgtc taatggaaga agaaggtatt cttgcaggta tctcttctgg agcagctgtt
     8041 gccgcggcgt tgaaactaca agaagatgaa agctttacca ataagaatat tgtggttatt
     8101 ctaccatcat cgggtgagcg ttatttaagc accgcattgt ttgccgatct cttcactgag
     8161 aaagaattgc aacagtaatg ccagcttgtt aaaaatgcgt aaaaaagcac ctttttaggt
     8221 gcttttttgt ggcctgcttc aaactttcgc ccctcctggc attgattcag cctgtcggaa
     8281 ctggtattta accagactaa ttattttgat gcgcgaaatt aatcgttaca ggaaaagcca
     8341 aagctgaatc gattttatga tttggttcaa ttcttccttt aacggcataa tgtttaatga
     8401 cgtacgaaac gtcagcggtc aacacccgcc agcaatggac tgtattgcgc tcttcgtgcg
     8461 tcgcgtctgt taaaaactgg cgctaacaat acaggctaaa gtcgaaccgc caggctagac
     8521 tttagttcca caacactaaa cctataagtt ggggaaatac aatgttccag caagaagtta
     8581 ccattaccgc tccgaacggt ctgcacaccc gccctgctgc ccagtttgta aaagaagcta
     8641 agggcttcac ttctgaaatt actgtgactt ccaacggcaa aagcgccagc gcgaaaagcc
     8701 tgtttaaact gcagactctg ggcctgactc aaggtaccgt tgtgactatc tccgcagaag
     8761 gcgaagacga gcagaaagcg gttgaacatc tggttaaact gatggcggaa ctcgagtaat
     8821 ttcccgggtt cttttaaaaa tcagtcacaa gtaaggtagg gttatgattt caggcatttt
     8881 agcatccccg ggtatcgctt tcggtaaagc tctgcttctg aaagaagacg aaattgtcat
     8941 tgaccggaaa aaaatttctg ccgaccaggt tgatcaggaa gttgaacgtt ttctgagcgg
     9001 tcgtgccaag gcatcagccc agctggaaac gatcaaaacg aaagctggtg aaacgttcgg
     9061 tgaagaaaaa gaagccatct ttgaagggca tattatgctg ctcgaagatg aggagctgga
     9121 gcaggaaatc atagccctga ttaaagataa acacatgaca gctgacgcag ctgctcatga
     9181 agttatcgaa ggtcaggctt ctgccctgga agagctggat gatgaatacc tgaaagaacg
     9241 tgcggctgac gtacgtgata tcggtaagcg cctgctgcgc aacatcctgg gcctgaagat
     9301 tatcgacctg agcgccattc aggatgaagt cattctggtt gccgctgacc tgacgccgtc
     9361 cgaaaccgca cagctgaacc tgaagaaggt gctgggtttc atcaccgacg cgggtggccg
     9421 tacttcccac acctctatca tggcgcgttc tctggaactg cctgctatcg tgggtaccgg
     9481 tagcgtcacc tctcaggtga aaaatgacga ctatctgatt ctggatgccg taaataatca
     9541 ggtttacgtc aatccaacca acgaagttat tgataaaatg cgcgctgttc aggagcaagt
     9601 ggcttctgaa aaagcagagc ttgctaaact gaaagatctg ccagctatta cgctggacgg
     9661 tcaccaggta gaagtatgcg ctaacattgg tacggttcgt gacgttgaag gtgcagagcg
     9721 taacggcgct gaaggcgttg gtctgtatcg tactgagttc ctgttcatgg accgcgacgc
     9781 gctgcccact gaagaagaac agtttgctgc ttacaaagca gtggctgaag cgtgtggctc
     9841 gcaggcggtt atcgttcgta ccatggacat cggcggcgac aaagagctgc catacatgaa
     9901 cttcccgaaa gaagagaacc cgttcctcgg ctggcgcgct atccgtatcg cgatggatcg
     9961 taaagagatc ctgcgcgatc agctccgcgc tatcctgcgt gcctcggctt tcggtaaatt
    10021 gcgcattatg ttcccgatga tcatctctgt tgaagaagtg cgtgcactgc gcaaagagat
    10081 cgaaatctac aaacaggaac tgcgcgacga aggtaaagcg tttgacgagt caattgaaat
    10141 cggcgtaatg gtggaaacac cggctgccgc aacaattgca cgtcatttag ccaaagaagt
    10201 tgatttcttt agtatcggca ccaatgattt aacgcagtac actctggcag ttgaccgtgg
    10261 taatgatatg atttcacacc tttaccagcc aatgtcaccg tccgtgctga acttgatcaa
    10321 gcaagttatt gatgcttctc atgctgaagg caaatggact ggcatgtgtg gtgagcttgc
    10381 tggcgatgaa cgtgctacac ttctgttgct ggggatgggt ctggacgaat tctctatgag
    10441 cgccatttct atcccgcgca ttaagaagat tatccgtaac acgaacttcg aagatgcgaa
    10501 ggtgttagca gagcaggctc ttgctcaacc gacaacggac gagttaatga cgctggttaa
    10561 caagttcatt gaagaaaaaa caatctgcta atccacgaga tgcggcccaa tttactgctt
    10621 aggagaagat catgggtttg ttcgataaac tgaaatctct ggtttccgac gacaagaagg
    10681 ataccggaac tattgagatc attgctccgc tctctggcga gatcgtcaat atcgaagacg
    10741 tgccggatgt cgtttttgcg gaaaaaatcg ttggtgatgg tattgctatc aaaccaacgg
    10801 gtaacaaaat ggtcgcgcct gtagacggta ccattggtaa aatctttgaa accaatcacg
    10861 cgttctctat cgaatctgat agcggcgttg aactgttcgt ccacttcggt atcgacaccg
    10921 ttgaactgaa aggcgaaggc ttcaagcgta ttgctgaaga aggtcagcgc gtgaaagttg
    10981 gcgatactgt cattgaattt gatctgccgc tgctggaaga gaaagccaag tctaccctga
    11041 ctccggttgt tatctccaac atggacgaaa tcaaagaact gatcaaactg tccggtagcg
    11101 taaccgtggg tgaaaccccg gttatccgca tcaagaagta attcttgccg cagtgaaaaa
    11161 tggcgccca
//
LOCUS       DAZPEP010000138        10929 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZPEP010000138 DAZPEP010000000
VERSION     DAZPEP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.138"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            98..595
                     /gene="ftnA"
                     /locus_tag="ACHUT9_003382"
     CDS             98..595
                     /gene="ftnA"
                     /locus_tag="ACHUT9_003382"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFR1349784.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(633..872)
                     /locus_tag="ACHUT9_003383"
     CDS             complement(633..872)
                     /locus_tag="ACHUT9_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFR1349785.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1063..2274
                     /gene="tyrP"
                     /locus_tag="ACHUT9_003384"
     CDS             1063..2274
                     /gene="tyrP"
                     /locus_tag="ACHUT9_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFR1349786.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2336..3001)
                     /gene="yecA"
                     /locus_tag="ACHUT9_003385"
     CDS             complement(2336..3001)
                     /gene="yecA"
                     /locus_tag="ACHUT9_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFR1349787.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3197..3283)
                     /locus_tag="ACHUT9_003386"
     tRNA            complement(3197..3283)
                     /locus_tag="ACHUT9_003386"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3247..3249),aa:Leu,seq:taa)
     gene            complement(3296..3369)
                     /locus_tag="ACHUT9_003387"
     tRNA            complement(3296..3369)
                     /locus_tag="ACHUT9_003387"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3335..3337),aa:Cys,seq:gca)
     gene            complement(3424..3499)
                     /locus_tag="ACHUT9_003388"
     tRNA            complement(3424..3499)
                     /locus_tag="ACHUT9_003388"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3464..3466),aa:Gly,seq:gcc)
     gene            complement(3651..4199)
                     /gene="pgsA"
                     /locus_tag="ACHUT9_003389"
     CDS             complement(3651..4199)
                     /gene="pgsA"
                     /locus_tag="ACHUT9_003389"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFR1349788.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4256..6088)
                     /gene="uvrC"
                     /locus_tag="ACHUT9_003390"
     CDS             complement(4256..6088)
                     /gene="uvrC"
                     /locus_tag="ACHUT9_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFR1349789.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6085..6741)
                     /gene="uvrY"
                     /locus_tag="ACHUT9_003391"
     CDS             complement(6085..6741)
                     /gene="uvrY"
                     /locus_tag="ACHUT9_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFR1349790.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            7037..7213
                     /gene="yecU"
                     /locus_tag="ACHUT9_003392"
     CDS             7037..7213
                     /gene="yecU"
                     /locus_tag="ACHUT9_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFR1349791.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7200..7424
                     /gene="yecF"
                     /locus_tag="ACHUT9_003393"
     CDS             7200..7424
                     /gene="yecF"
                     /locus_tag="ACHUT9_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFR1349792.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7491..8213)
                     /gene="sdiA"
                     /locus_tag="ACHUT9_003394"
     CDS             complement(7491..8213)
                     /gene="sdiA"
                     /locus_tag="ACHUT9_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFR1349793.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(8443..9195)
                     /gene="tcyN"
                     /locus_tag="ACHUT9_003395"
     CDS             complement(8443..9195)
                     /gene="tcyN"
                     /locus_tag="ACHUT9_003395"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFR1349794.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(9192..9860)
                     /gene="tcyL"
                     /locus_tag="ACHUT9_003396"
     CDS             complement(9192..9860)
                     /gene="tcyL"
                     /locus_tag="ACHUT9_003396"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFR1349795.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(9875..10861)
                     /gene="dcyD"
                     /locus_tag="ACHUT9_003397"
     CDS             complement(9875..10861)
                     /gene="dcyD"
                     /locus_tag="ACHUT9_003397"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFR1349796.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT         2711 a         2838 c         2577 g         2803 t
ORIGIN      
        1 ttaataattg aagtttatat tttacctttt gcaaataata aaataacaaa ttatagtgac
       61 gccacagcaa caaatataac ctttgtggag cactatcatg ctgaaaccag aaatgattga
      121 aaaacttaat gagcagatga acctggaact gtactcttca ctgctttatc agcaaatgag
      181 cgcctggtgc agctatcata ccttcgaagg tgctgccgcg ttcctgcgcc gtcacgccca
      241 ggaagagatg acgcatatgc agcgtctgtt tgattacctg actgataccg gcaatttacc
      301 gcgtattaat accgttgaat ctccgtttgc tgaatattcc tcacttgatg aattattcca
      361 ggaaacctat aaacacgaac aattaatcac ccagaaaatt aacgaactgg ctcatgctgc
      421 aatgaccaat caggactacc caacatttaa tttcctgcag tggtatgttt ctgagcagca
      481 tgaagaagag aaactgttca aatcgattat tgataaatta agcctggcag gcaaaagcgg
      541 cgaaggtctg tattttatcg acaaagaact ctctaccctc gacacacaaa actaatgctc
      601 tccggcggca ggtttactgc cgccgtttca aatcagtgac ggcaaatctt actctgatcg
      661 gtcgaaaaac cgtctttcga aggtataaat ttgccttttg ctgccagaaa cgctaccagc
      721 tctcccgctg tcatcccttc tgccgagcag gtgtgaaaac gtgcctgttc accaaaacgc
      781 gctttaatcg cagcttccag actggcatgc gtatattgct cgcctgattc aatcatcata
      841 tttaacactt catgaccgtg aatagagtcc atcgttcctc ctcaaaaaaa gcctagcgta
      901 gcgattgccg cttatgaaga ctttgcgcca gcgcaggact gaatgctttt tattgtacat
      961 ttatatttac actatatgta acgtcggttt gacgaagcag ccgttatgcc ttaacctgcg
     1021 ccgcagatat cactcataaa gatcgtcagg acagaagaaa gcgtgaaaaa cagaaccctg
     1081 ggaagtgttt ttatcgtggc gggaaccaca attggcgcag gcatgctggc aatgccgctg
     1141 gctgcggccg gtgttggttt tagcgttacg ttaatcttgt tgattgggct ttgggcgttg
     1201 atgtgctaca cggcgctatt actgctggag gtgtaccagc atgttccggc agataccggt
     1261 ctgggcacgc tggcaaaacg ctatctggga cgctacggtc aatggctgac gggcttcagt
     1321 atgatgttct taatgtatgc tctgactgcg gcatacatca gcggtgtcgg tgaattgttg
     1381 gcctccagca tcagcgactg gacaggtatt tcaatgtcgg caaccgctgg cgtgctgttg
     1441 ttcacttttg ttgccggtgg cgtggtttgt gttggaacat cgctggtcga tttatttaac
     1501 cgttttctgt tcagcgccaa aattattttt ctggtggtga tgctggtatt gctgctaccg
     1561 catattcaca aagtgaatct tttaaccctg ccgttgcaac aggggctggc tctgtcagca
     1621 atcccggtga tttttacctc atttggtttt cacggtagcg tgccgagtat tgtcagctat
     1681 atggatggca acgttcgtaa gctacgctgg gtgtttataa ccggtagtgc gatccccctg
     1741 gtggcatata ttttctggca ggtggcaacg cttggcagca ttgattcaac aacctttatg
     1801 ggattgctgg ctaatcatgc tggattaaac gggctgttac aggcgttacg cgaaatggtg
     1861 gcctctccgc atgttgagct ggcagtgcat ttatttgctg atttagccct cgccacgtca
     1921 tttctcggcg ttgcgttagg cttatttgat tatctggctg atttatttca gcgttcaaat
     1981 accgttggtg gacggttgca aactggtgca attacctttc tgccgccgtt ggcgtttgca
     2041 ctgttttatc cacgaggatt tgtgatggcg ctgggttacg ccggtgtggc gctggcggta
     2101 ctggcattga ttatcccttc gctgttgacc tggcaaagca gaaagcacaa tcctcaggcg
     2161 ggttaccggg tcaaaggtgg tcgtccggcg ctggtggtgg tgtttctctg tggtattgct
     2221 gtgattggcg tgcaattttt gattgcggca gggttgttac cagaagtggg gtgatcagat
     2281 agcctcaaat tccttattgg gtgccagaat taacgctgac acccaatttg gcctcttaat
     2341 gcaggcagca ctgcttaaat ttcttaccac taccgcacgg gcaaggatcg ttacgccccg
     2401 gtttctcttc tgctttgatc ggttgctgaa cagctttttc ctgcggatgc gccatccagt
     2461 acgcatgtag atcaagcgcc gccagtcgaa tggcatctac gctctcttca aacgcttctg
     2521 gcgacatctt ttctacccgc tcgaagtttt cctcagtacc gtgcagcgca atcgcctcca
     2581 gcgctggttt taacgaatcg ggcaacgttg accagtcaga aagtgccacg ccccgcatat
     2641 agccaaagca ccactcctca acaatcgtca gctcgctgcc atcaacttct cgcaagccga
     2701 ataacggctc aaactgctcc gggaattcgt tcagacgctc tgcggtatcg gccatatgtt
     2761 gaaaagccag gttcataaag cgcgtcatct ctttctctga cgcccagcgc ggcacatagt
     2821 cagccccacc ccacacggca accagccact gttccggttc aatctcttgc ggagaactca
     2881 acaccgccgt caataaaccg tccagctccg ccacatcaag gatggcgtgg ttagtgttgt
     2941 atttggtcag aatatcgtcc agccattcca actcactttc gtttaacggt cccgttttca
     3001 tacgcttttc cttgtggatc tcaactcgcc agcacctatc ttacatgccg gtccgtatca
     3061 gagatacttt ttgagtggct ttgccggtga ttaaaaatta aggagggtgt aacgacaagt
     3121 tgcaggcaca aaaaaaccac ccgaaggtgg tttcacgaca ctgcttattg ctttgatttt
     3181 attcttatct ttcccatggt acccggagcg ggacttgaac ccgcacagcg cgaacgccga
     3241 gggattttaa atcccttgtg tctaccgatt ccaccatccg ggctcgggaa gaaagtggag
     3301 gcgcgttccg gagtcgaacc ggactagacg gatttgcaat ccgctacata accgctttgt
     3361 taacgcgcca aattcttcag gcctttcagc cagacatccg cttgacgccg atgtctttta
     3421 aactggagcg ggaaacgaga ctcgaactcg cgaccccgac cttggcaagg tcgtgctcta
     3481 ccaactgagc tattcccgca ttcatcaagc aatcagttaa tcacttgatt ttattatcgt
     3541 ctggcaatca gtgccgccgt tcgatgcgtt gcattctact tacctggcgc gatgagtcaa
     3601 cgatattttt caccactttt gatcgtttgc tgaaaattac gccgaaacga tcactgatca
     3661 agcaaatctg cacgcgcagc gctcaaatat tgcaacattg accacagagt cagtaccgca
     3721 gccacaaaga aaagtgcaat accggcgtac tcaacccaaa tgttcggacg ccacagcagc
     3781 catgccaacg ccaccatctg ggcagtggtt ttcactttcc caatccagga gacagccacg
     3841 ctactgcgtt tacccaactc cgccatccat tcgcgtagcg cagaaataat aatttcacgg
     3901 gcgatcatcg ttgctgccgg taaggtcacc caccagctgt ggtaatgctc ggttaccagc
     3961 accatggcga tagccacgag aactttatct gctacagggt caaggaaagc accaaaccgg
     4021 gtactctggt tccagcggcg tgccagaaaa ccatcgaacc agtcagtcac cgccgcgacg
     4081 cagaaaatga gcgcggcggc aaacggcgac caggtgacag gcagataaaa gaccaataca
     4141 aagaatggga taaggatgac acggaacagt gtaagcaacg tagggatatt aaattgcata
     4201 atgacgggta actatctgtt gtcagtaaga ttacccctat gttgctacag agacatcaat
     4261 gtttcaacga ccagaagatc ttttctgcca gaccttgcga aatacccggc acttttgcaa
     4321 tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc catatatttc aacaacattt
     4381 gccgacgttt tggcccgacg ccttcaatgg tttccaggga actggtattt ttgaccttcg
     4441 cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga ttcatcgcga atatgctgga
     4501 taacatgcag cgcgggagaa tctggcggca aactgaatcc ctcaccttcc ggctcaaaga
     4561 acagcgtttc cagcccagcc ttacgatctg ctcctttggc aacgccaagt agcagcggat
     4621 gatttttatc ccatgagaca tccagttcgg cgaagacatt tttcgcctgc gcaagctggc
     4681 ctttgccgcc gtcgataagg atcacatccg ggatcttact gtcgtcaatg gctttaccat
     4741 aacgccgacg cagcacctga ttcatcgccg cataatcatc gcccggcgtg atgcctgtaa
     4801 tgttatagcg ccgatactcc gcacgcagcg ggccgttagc atcaaacacc acacaggaag
     4861 cgacggtttg ttcgcccatg gtatggctga tgtcaaagca ctccatccgc ttcacttccg
     4921 gcaatttcaa cacgctggca agcgcggtca gtcgctggtg aacggtagat tgctgcgaaa
     4981 gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag tttcagataa cgcgccctat
     5041 cgccgcgagg tttggtttga acattaatct tgcgtcccgc cagttctgaa agggaatcgg
     5101 cgagcagcgt tttatcgcta agattaaaat cgagcaggat ctcacccggt aaggtgcgca
     5161 tctggctgcc ttgtaaatag aactggccta cgaaggtttc taccacctcg ctcagttccg
     5221 taccgccagg cactttcggg aaatagctgc ggctgccgag cactttgccc tgacgaatga
     5281 acaatacgtg gacacaagcc atgcccgcat cgaacgccac accaataacg tcgaggtcgt
     5341 cgccggtatt ggaaacgaat tgtttttcgg tgacgcgtcg caccgcctga atttggtcgc
     5401 gaatacgtgc agcttcttca aactccagat tctggctggc agtttccata cgactaatga
     5461 gttgcgtaag cacctgatca tctttgccag acaaaaacag gcgcacatac tcaacctgct
     5521 gagcgtattc ttcttcactc accagtcctt caacgcacgg tcccagacag cgccctatct
     5581 gatattgcag acacggacgc gagcgattgc gataaacact attttcgcac tggcgaatgg
     5641 ggaaaatctt ttgcagtagc gccagtgttt cacgtacggc atagccattc gggaacgggc
     5701 cgaaatattc acctttggca tgcttcgcac cacgatgcat cgccagacgc gggtgggtat
     5761 cgccactcag gaagataaaa ggataagatt tatcatcgcg tagcaaaacg ttgtaacgcg
     5821 gctgatagag tttgatgtag ttgtgttcca gcaacagcgc ttcggtttct gtatgcgtaa
     5881 ccgttacatc aatttgctgg atctgggcga ccagcgcttc ggttttgcgc gaagcgaggt
     5941 tgctgcggaa atagctggaa agccgttttt tcaggtcttt cgctttgccg acatagataa
     6001 ccgtaccacc agcatcgtac atgcgataaa cgcctggctg gctggttacg gtttttaaaa
     6061 acgcttttgc gtcaaactga tcactcactg acttgataat gtctccgcat tacacagacc
     6121 atggcgaatt gccaggtgag tcagctcaac atcgccatga atgtttagtt tactgaacat
     6181 acgatagcgg tagctgttca ccgttttcgg actgagattg agctgttctg agatctcatt
     6241 gaccttctgg cccttggtga tcatcagcat aatctgcaat tcacgttcag acaaactggc
     6301 aaatgggctt tctgtttttt ctggttcgat ctggcttaac gccatttgtt gagcgatatc
     6361 agaagcaatg taacgctgcc ctgaatagac agaacgaatc gcactcacga cttcctgcgg
     6421 agccgcgcct ttgctgaggt agcccgcagc accggcctgc atgactttcg ctggtaaagg
     6481 gttttctgta tggacggtaa gcatgatgat tttgacatca gctgtggaac gcgcgatttt
     6541 acgcgtcgcc tcaagaccgc caatgcccgg catactcatg tccattagca ccacgtcaac
     6601 ggcatttgtc cggcaccact taacggcgtc ttcaccgcac gatgcctcac cgacgacttt
     6661 tatacccttt atatcttcca gaatgcgtcg tatccctgcg cgcaccagtt cgtggtcatc
     6721 aacaagtaga acgttgatca aaggaatatc tccagaaata gggataacgc tactgatagt
     6781 tagtcattcg tatattagcg gtttttattg caactttgaa acgttaaaaa tggtgggtat
     6841 tcgatttttc tctcgttttt gtaaattcga ttgtccacag ttatggtcac gcccgtcacc
     6901 aacggcttcc gggatcgttt cacagtaagc ccggcgatga aatgtgacaa aaattgactt
     6961 tattcagcaa aaatgaaaat cagcccgctg aatatgtaac attaattaac ccgaacaata
     7021 cgggcaaaaa acattcatga taaaaatatt tattggtcat tatattaacg catttaatag
     7081 cattgttgac gtcacgctca aaaaacaacc actgctattt ttagcaaagc ttatggtata
     7141 ctccgccgcc ttaacatttt tcaccgcaaa ttttcattgc aacatgacga ggaaaataaa
     7201 tgagtacgcc tgatttttct actgccgaga ataatcaaga actggcaaat gaagtctcct
     7261 gcctgaaagc gatgctgacg ctgatgctac aggcgatggg acaagctgac gcgggccgcg
     7321 tgatgttaaa gatggaaaaa cagcttgcgc tgatcgaaga cgaaacccag gctgcagtat
     7381 tttccaaaac ggttaagcaa attaaacaag cctaccgtca gtaatataaa accggctgag
     7441 agcgtgcctt tcagccgatt tttgcatctg gcacgcagaa agaaaagcga tcaaattaag
     7501 ccagtagcgg ccgcgtaaca ggccacctgg gtcttatttg gtgcattgat ttttttctgc
     7561 atgtttttct gatggaaatt gaccgtattc tcagagattg acaaaatcat cgctatctct
     7621 gctgatgtct tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc
     7681 atctctggcg tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc
     7741 accagtaact gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg
     7801 caacgggaaa aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca
     7861 ccgcggcgta aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag
     7921 tcatcattcc acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt
     7981 gcgagaaaat tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa
     8041 gccactttag gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac
     8101 tccagctgct gagcctgaag ctctatttca tggtagacct cttctgcggc ctccatcctc
     8161 tgaaaacgca acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac
     8221 cgcaacgccc ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa
     8281 gatttatata taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct
     8341 cttatgttat taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg
     8401 tcccgccccg acaggtgaat cgtcggggct gattttttct tattattgca gcagaaactt
     8461 ctcgaggaac tggcgggtgc gaggctgctg gggatcagca aataacgctt ttgctgcccc
     8521 ctgctcgact atccgcccct ggtccataaa aatcgcccgg tccgcaacat cccgggcaaa
     8581 gctcatttcg tgcgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggattgt
     8641 gttcaggact tcgcccacca gctctggatc cagcgctgac gttggctcgt caaacaaaat
     8701 cacctcagga cgcattgcca gcgcacgcgc aatcgctaca cgctgctgtt gaccgccaga
     8761 caaacgacgt ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg
     8821 agcgcgcgcc gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat
     8881 gttttccagc accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg
     8941 ctgacgcaac tggcggatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt
     9001 gatatcgcca acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctgcgtag
     9061 caacgtggtt ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac
     9121 ctcgaggtcg ataccgtgca gcaccgtctg accgtgaaat tttttcacca ggttcttaac
     9181 ttcaatggca ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc
     9241 tgcaacgtcg ataatactgt cgccatgatc cagtagatga gcgaagccgc caggtacatg
     9301 gtgaacacct ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc
     9361 tggattgtcg cggccagcga cgtatccttt accaggctga taaagctgtt cgacagcggc
     9421 ggcaacgcca cccgcgccgc ctgtggcaaa atcgcgcgac gcatagtctg ccatggcgtc
     9481 ataccaatac tggcagctgc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc
     9541 agtgtttcgg cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg
     9601 ggatctaatt cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg
     9661 agcggtgtac cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt
     9721 ggcgacaggc gcatcagcgc gagaataaac ccgagcagta agccaaaaaa catgccgccg
     9781 atactgagtt gcagcgtata cccagcccct ttcagcagga acggcaagga atcaataacc
     9841 agttgtatac tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca
     9901 gcgcaggcgc accgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct
     9961 gactgataca gccaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa
    10021 tgccttccag ccgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt
    10081 agccaggtgc aaaatagtca tcccagagta taatttccgc tgatgcggtc agctccagtt
    10141 ctttcgcaat cgcctgttgt aggttaacca ctttcggcaa ctgatcggca acggaacgcg
    10201 acacggtcac gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc
    10261 cggcgtgagt tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt
    10321 cacactgttg cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag
    10381 aaccgccaac cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagct
    10441 cttccagttg ggcattggga tcggtcagtg cttcgcacat ttcaatctgg gtattgaaca
    10501 gatccagcaa caagcgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat
    10561 tttccagcag cgccacacag tgcagaccga gtttcgccgc aactgcggca gtctgccgca
    10621 catggttaga ctggatcacc ccggcagtaa tcagcgtatc ggcaccttcg cgcagagcat
    10681 ctgccgcgag aaattctagc ttacgtaatt tattgccgcc cattgccatg ggggtgacgt
    10741 catcccgttt gatgaaaatt tcccgtccta gataatcaga aaagcgcggc agatattcga
    10801 gcggcgttgg cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca
    10861 taacagcctc cgatgtatgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa
    10921 taaaaaagg
//
LOCUS       DAZPEP010000139        10834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZPEP010000139 DAZPEP010000000
VERSION     DAZPEP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.139"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..279)
                     /locus_tag="ACHUT9_003398"
     CDS             complement(<1..279)
                     /locus_tag="ACHUT9_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1349797.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(446..1186)
                     /locus_tag="ACHUT9_003399"
     CDS             complement(446..1186)
                     /locus_tag="ACHUT9_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFR1349798.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHUT9_003400"
     CDS             complement(1279..1914)
                     /gene="argO"
                     /locus_tag="ACHUT9_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFR1349799.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHUT9_003401"
     CDS             complement(2053..2913)
                     /gene="mscS"
                     /locus_tag="ACHUT9_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFR1349800.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHUT9_003402"
     CDS             complement(3104..4183)
                     /gene="fbaA"
                     /locus_tag="ACHUT9_003402"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFR1349801.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHUT9_003403"
     CDS             complement(4398..5561)
                     /gene="pgk"
                     /locus_tag="ACHUT9_003403"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFR1349802.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHUT9_003404"
     CDS             complement(5611..6630)
                     /gene="epd"
                     /locus_tag="ACHUT9_003404"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFR1349803.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7002..7433
                     /locus_tag="ACHUT9_003405"
     CDS             7002..7433
                     /locus_tag="ACHUT9_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFR1349804.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7456..8034
                     /locus_tag="ACHUT9_003406"
     CDS             7456..8034
                     /locus_tag="ACHUT9_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFR1349805.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8035..8742
                     /locus_tag="ACHUT9_003407"
     CDS             8035..8742
                     /locus_tag="ACHUT9_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFR1349806.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8730..9407
                     /locus_tag="ACHUT9_003408"
     CDS             8730..9407
                     /locus_tag="ACHUT9_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1349807.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9401..10078
                     /locus_tag="ACHUT9_003409"
     CDS             9401..10078
                     /locus_tag="ACHUT9_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFR1349808.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10050..10763)
                     /locus_tag="ACHUT9_003410"
     CDS             complement(10050..10763)
                     /locus_tag="ACHUT9_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFR1349809.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10760..>10834)
                     /locus_tag="ACHUT9_003411"
     CDS             complement(10760..>10834)
                     /locus_tag="ACHUT9_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000209973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1349810.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2581 a         2879 c         2698 g         2676 t
ORIGIN      
        1 tcgtgaacga cgccctgagt tatggatttc attatcaatc gcagaaagtc gttgataaac
       61 cgggaacagt ttttgataca gttcctgacc ggctttattg aggctgatat tattatcttt
      121 gcgggtaaat agcgtatagc caatttgttc ttccagcgcg gcaatgcttt taccaaatgg
      181 cgaagccgtc atatggattt tttctgccgc ccgggcaata ttattggttt gcgctaataa
      241 aatgaaattg cgcatctttt tcgaaataaa aatgtccatc ccccctcccc gcatccttgt
      301 ccgcttaacc gtttcagtca gcctatcctt gatgaaaccg cgagcaaaga taggtgatta
      361 cgtcatggtt ttacagaaaa ttacagaaaa aggaggcaat atcgggtaaa ggcattagcc
      421 cgacgaatac gtcgggctac aagtattatt gtgctgcagg tgttttagcg ggttgttgat
      481 ccacaggttc taactggaag accacatcga cctgatcatc aaactgaata gcggcctgct
      541 cgtaagtttc ctgggcagac accggcgcgg catcggcttt catcatccgc accattgggc
      601 tgggctgata gttggaaaca tggtagcgca cgctatatac cggccccagt ttacgatgaa
      661 agccgttcgc cagttcctgc gcctgatgaa tcgcgttatc aatcgctgcc ttacgcgctt
      721 tgtctttata ggcatccggc tgcgccacgc ccagcgacac agaacgaatt tcgttaagac
      781 ccgccttcag cgcgccatcc agcaaggaat tcagtttgtc taactgacgg agcgtgactt
      841 ccaccgttct cacagcgcgg tagcctttca ggatactttt accatcctga taatcataat
      901 ctggctgggt gcgtaagttc gctgagctga tatctttttt cgcgatctga ttgagttcaa
      961 ggaaggaaat gtattgtgcg acgcgctcat ctgcctgttt cttggctgta gcggcatcct
     1021 tcgcggccac gttaacttca atcgcaagag tggcaatgtc tggcaccgca tccacacttg
     1081 ccgtaccgga ggtgacaata tgcggtccat ccggcaattc gttagcctgc gctgccatcc
     1141 cgctaatacc cattaatgcc gccagggcga taactttgaa cttcacgtta gtctctccgt
     1201 gttaattgac ttgcccaaaa ttcaggacct ctggaggcaa gcttagcgcc tctgttttat
     1261 ttttccatca gatagcgcct aactgaacaa ggcttgtgca tgagcaatac cgtctctcgc
     1321 cagctgcaag gctataaacc acatgacaca tcccactacc agattgataa tgcgctgtga
     1381 ttttgccgtg cgcaggcgcg gtgccagcca ggctgcgaga atagccagac caaagaacca
     1441 caggaaagag gcgctaattg tcccgagcgc aaaccaacgt tttggttcga cgtcaagctg
     1501 cccgccaagg ctgcccagta caacaaaagt atccaggtaa acatgcggat tcagccaggt
     1561 cactgccagc atggtggcga taattttcca tctgccttgc ttcaggactt cggcactagc
     1621 taactcaata ttactactca tcgctgtttt aaaagcgcca aaaccatacc acagcaagaa
     1681 ggctacgccg ccccaggtga ccagcgccag caaccacggc gactgcatca ataacgcgct
     1741 gccaccaaaa atcccggcgc aaatcaggac caaatcgctg atagcacaaa gtaaggcaat
     1801 cataatgtgg tactgacgac gaatgccctg attcatcaca aaagcatttt gtggaccgag
     1861 cggcagaatc atagctgccc caagagcaat accttgaaag taataagaaa acacgttgga
     1921 tattccgaat taagtatctg atgcagagac tatatcgcgg gattgttatt agcggaaatt
     1981 gataatatta attggcaatc agaataacta ataagagaga ggccagagag tattatctgg
     2041 cctcagcgtt gattacgcag ctttgtcttc tttcacccgc ttaaagttca catccatttg
     2101 cgggtacggg aagctgatac cggcggcatc aaattcacgt ttaatacgct ccagcacatc
     2161 ccagtacacg ttttgcagat cgccgctgtt gctccagacg cggaccacga aattaatcga
     2221 cgatgcacca agttcgttca gacgcacagt catttcgcga tctttcaaaa tgcgatcttc
     2281 agactggata atattggtca ggatctgctt aacctgatcg atatcggaat catacgccac
     2341 gccaataata aattcgttac ggcgaactgg ctcgcgagag aagttaataa tatttccggc
     2401 aataatttta ccgttcggaa taacgataat tttaccgtct gcagtacgca tggtggtgga
     2461 gaaaatctgc acactcagca cagtaccggc tacgccgccg agatcaacat attctccggc
     2521 acggaacggg cggaacatga caagtaacac gccagcggcc aggttagaaa gtgacccctg
     2581 caaagccaga ccaacagcta agcctgcggc accgagtaca gcaatgactg acgctgtttg
     2641 tacacccacg cgtcccaggg cagcgattag cgtaaaggcg ataataccgt aacggactaa
     2701 tgcagaaaga aaatcagcaa cagtggcatc gattttacgg gagatcatca ggcgattcac
     2761 cgcgttggaa atcatccgcg cgataatcaa accaacgatg atgatcgcga gtgccgccac
     2821 gatgtttact gcataactta gcagcagcgc ctggttagct accagccagc ttcccgcgcc
     2881 gtttatgcta tcgacaacat tcaaatcttc cattcaatat tccttttcaa ctgactcaaa
     2941 atggagaaat acactccgcc gccttatgac gggcagtctg acaagggtaa acaataaagc
     3001 ataattttgc caaatagatc acagatttct catctgaatc accttgcttt tggcgaaaaa
     3061 aagacccgca gagcgggcct tgagataagc agaaaggaat atcttacaga acgtcgatcg
     3121 cgttcagttc ctggaatgct ttttccagac gagcgatcat cgaagtctga ccggcacgca
     3181 gccatacgcg cggatcgtag tatttcttgt tcggctgatc ttcgcctttc gggttaccca
     3241 actgcccctg cagataagct tcgttcgctt tgtagtagtt cagaacgcct tcccaggttg
     3301 cccattgggt atcggtatcg atgttcattt ttactacgcc gtagcttacg gagtctttga
     3361 tttcctgagc agtagaaccg gaaccaccgt ggaatacgaa gttcaggctg ttgtgcggca
     3421 ggttgtgttt cttggaaaca tattcctgag aatcacgcag gatggtcgga gtcagaacca
     3481 cgttacccgg cttgtaaaca ccgtgtacgt taccgaagga cgcagcgatg gtgaaacgcg
     3541 ggctgatttt gctcagttcg gtgtatgcgt aatcaacgtc ttccggctgg gtgtacagtg
     3601 cagaagcgtc catgtggctg ttgtccacgc cgtcttcttc accaccggtg caacccagtt
     3661 cgatttccag agtcatgccg attttggaca tgcgctccag gtatttagag cagatttcga
     3721 tgttctcttg cagagattct tcagacaggt cgatcatgtg agaagagaac agcggcttac
     3781 cggtagctgc gaagtgtttt tcacccgcgt ccagcagacc gtcgatccac ggcagcagtt
     3841 tcttcgcgca gtggtcagtg tgcaggataa ccggaacacc ataatgttca gccatctggt
     3901 gaacgtgatg cgcaccagag atcgcgccca ggatagcagc accctgcgga acgtcagatt
     3961 tcacgccttt accagcgata aaggaagcac caccgttgga gaactgaacg ataaccggcg
     4021 ctttaacttt agcagcggtt tccagtacgg cgttgatgga gtcagtaccg acgcagttta
     4081 ctgctggcag tgcgaagttg ttttcttttg ctacctggaa aactttctgt acgtcatcac
     4141 cagtgattac gccaggtttt acgaaatcaa aaatcttaga catgtctctt gtcctgtatc
     4201 gtcgggcctt atactcatca tacttcaagt tgcatgtgct gcgtctgcgt tcgctcaccc
     4261 cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc ctgcaactcg
     4321 aattatttag agtatgaaaa aatgcgtgct ctaaaagcgc gctgaaacaa gggcaggttt
     4381 ccctgccctg tgatttttta cttcttagcg cgctcttcga gcatcgctac tgcaggcagt
     4441 actttacctt ccacgaattc gaggaatgcg ccgccgccag tggagatgta ggagattttg
     4501 tcagcaatgc cgaacaggtc gattgctgcc agagtgtcgc cgccgccagc gatggagaac
     4561 gcttcgctgt ctgcgatagc gttagccacg atttcagtac ctttgcggaa gttcgggaat
     4621 tcgaacacgc caaccggacc gttccacaga atggttttcg cattcttcag gatttcagcc
     4681 agttcctgag cggaagcatc accgatatcc aggatctgct cgtcagcttt cacatcgtta
     4741 acagatttca gggtagccga tgcagtttca gagaactcgg ttgctacgcg aacatcagac
     4801 ggaaccggga tgttgcaggt ggtcagcaga cgtttagctt cgtcaaccag gtcagcttcg
     4861 tacagggatt tacccacatc gtggccttgt gccgcgataa aggtgttagc gataccacca
     4921 ccaacaatca gctggtcagc gattttagac agggagtcca gaacggtcag tttggtagat
     4981 actttagaac caccaacgat agccaccatc gggcgagcag gttctttcag tgctttaccc
     5041 agcgcgtcca gttcagctgc caacagcggg cctgcgcacg caacatcagc gaatttaccg
     5101 ataccgtgag tagaagcctg cgcgcggtga gcagtaccga atgcgtccat tacgaacacg
     5161 tcacacagtg cagcgtattt tttggacagg gtttcgtcgt ctttcttctc gcctttgttg
     5221 aagcgaacgt tttccagaac aaccagttca ccttcagcaa cgtcaacgcc gtcgaggtaa
     5281 tctttaacca gacgaaccgg gttagacagt ttgtctttca ggtagttaac aaccggcagc
     5341 agagagaatt cttcgttgta ctcgccttcg gtaggacgac ccaggtggga agttaccatc
     5401 actttcgcgc cttgtttcag ggccagttcg atggtcggca gagaagcacg gatacgcgcg
     5461 tcgctggtta ctttcccgtc ttttactggt acgttcagat ccgcacggat aaatacacgt
     5521 ttcccagcaa gatccagatc ggtcatctta attacagaca tggtgaatcc tctcgttgat
     5581 tctaaaagtt ttgcagacgc tgcttgcgtc ttacctgaaa gcaacagtag ccatagctaa
     5641 cgtcgtgtcg agcattcggt tagcaaagcc ccattcgtta tcgcaccaga ccaacgtttt
     5701 gatcaggtgt gcgccactga cccgggtttg ggtgccatcg acaatggcac tgtgcggatc
     5761 gtggttaaaa tctacagaga ccaacggcaa ttccgtatag tcaactatac catgaaatgc
     5821 accttgtgct gctttttgca gcaacaggtt gacttcattg gcttttacag gtttcttcac
     5881 cgtcacgctt aaatcgattg ccgtcacatt tatggttggc acacgtaccg caatcgcttc
     5941 aaagcgatcg ttaaattgcg gaaaaaatcg tgtgataccg gcggccagtt tagtatcgac
     6001 cggaatgatc gactggctgg ctgcccgggt gcgacgcagg tcaggatggt atgcatcaat
     6061 aacctgttga tcgtgcatgg cggagtgaat tgtggtcaca gtgccggact caataccgta
     6121 cgcatcatct aacaatttga tgacgggaat tatgcaattc gtggtacagg aagcgttaga
     6181 aacgatgcgg tgttccgcac gaagttgatc ctgattgacg ccgtaaacaa cggtcgcgtc
     6241 gagatcgtta ctgccaggat gtgaaaagag cacttttttg gccccggcgg caatatgcgc
     6301 ttcgccatgc tcgcgggagc catatacgcc ggtgcagtcg aggactacat caacgccaag
     6361 ttcacgccag gggagcgatt gcagtgaacg ttcatgcaat acgcggatgg cgtcatcacc
     6421 aacaaaaagt tgatcgcgtt cctgtcgtac ttcccatgca aaacggccat ggctggtgtc
     6481 atatttcaac aaatgcgcca tgcccgcagc atccgccagt tcgttgattg ccaccacggt
     6541 aatttccgcc cggcgtccgg attcatacaa agcacgaacc acattacgcc cgatgcgacc
     6601 gaagccattt atcgctacgc gtacggtcat agatctcctg caaggttttc cctgagcaaa
     6661 tttgccagac agagtaatcc agcaaatcgt ccggggaaac cttacctgtc gcaaactgcg
     6721 actgattggt taattgtcga acatttaatc gactgaaacg cttcagctag gataagcgaa
     6781 acgtggaata aaaggaatga ttgtccagtc aaagaaaaca attatctgac ttacgtcacg
     6841 ttttcagccc cgcccctgtg gaatttattc cactttgcag aatcacgaat aagcgtagca
     6901 aaatcttttc gttgtgccgt cacaaaccct gacttatatc agaatatgac tgatgcagcg
     6961 tcgatattat ccgcaatacg tccgagtttc ggagtactcc aatgatgaca ttgccctacc
     7021 catattcttc ccccgcacgc ttttatgcac tgcgcctcct ccccgggcag gaagtttttt
     7081 cccagctaca cgcttttgca cagcagcatc aacttcatgc cgcctggatt gcaggctgta
     7141 ccggtagcct gactgacgtc gccttgcgct atgccgggca agaaggcacc acgctcctta
     7201 acggtacatt cgaagtgatc tcactaaacg gcacgctgga acagagtggt gagcatcttc
     7261 atctctgcgt ttccgatcca cacggcacga tgcttggcgg tcatatgatg cccggctgta
     7321 ccgtgcggac cacgcttgaa ctggtgatcg gtagccttga ggaactggcg tttagccgcc
     7381 aaccttgcgc gctttctggt tacgacgagt tgcatatttc ccccgttaaa taactacttt
     7441 caaagaggtt ttcttatggc gcgtagccac ttttccagtc aggcactggt gttgatcgtt
     7501 atatctatcg ccatcaatat gatcggcggg caactggcca gcatggtcaa attacctata
     7561 tttctcgact ccatcggcac acttattagc gcggtactgc ttggtccggt gatcggaatg
     7621 ctgaccggct tactgaccaa tttactctgg ggattgctga ccgacccgat cgccgccgca
     7681 tttgcacccg tcgcgatggt catcggcctg gtcgccggtt ggctggcgcg agcgggttgg
     7741 tttcgcaccc tgccaaaagt cgttgtcagc ggcgtgatta ttacgcttgc ggtaacggtc
     7801 gtcgctgtac cgctgcgtac tgcgctgttc ggcggtgtca ccggtagcgg agccgatttg
     7861 ttcgttgcct ggatgcactc gatggggcaa aacctggtgg agtcggtggc gattacggtg
     7921 ataggcgcga acctggtcga taaaattctt acagcggtga ttgtctggct tttactgcgc
     7981 cagctgccga ttcgcaccac gcgccatttt ccggcaatgg ctgccgtgcg ctaaatgcat
     8041 ccatttacct cattaacact gtgggcattg gcggcctgca ccacgcttat cctgcccgcg
     8101 cagaccattt tgccgatcta tagcgcggcg accttttttt gtcttattgc gctaaaggcc
     8161 acgcggcggc gggcaaaata tgtcgcctgg ctgatgttct cgctgggagc cgggctgtgg
     8221 ctggtacacg gtggctggct aacagaatgg ctaagcggca cgccgcgttc gccggagcgt
     8281 tggacgcatg ctattacgct ctggttgcgg attctggcga ttgtttcaac ctcacagttg
     8341 tggatgcagt acgtccccgt gcaatgtttt atccgcgctc tgtttgcttc tcgcctgccg
     8401 ccaggcgtcg cttatctgtt tgccggaccg ttattggtcg ttgagcagtt aaaacggcaa
     8461 ttagcgatta ttcatgaagc gcagcgtgcc cgcggcgtgc cgctggatga gggctggtat
     8521 cagcgtttac gtgcgatgcc cgcgttaatt attctgctaa cacacaatgc gcttaacgat
     8581 ctggctgtcc gtggtgcggc gctggatatg cgggcatttc ggatccacaa ccgacgaaca
     8641 acgttgtggg caccagccga tagcacccta caacgcgtag cgcgttacac catgatcttg
     8701 ctgatgctaa cagaattcgg agcgtggata tggttacgtt agaacagttc cgttattgcc
     8761 cgacacactc aacgcatcct ccgttctgtt atgactttca ctatgtcaaa ccggggatgg
     8821 tcgccatctt tggtgacaac ggcagtggca aaagtacgct ggcacagctg atggcgggct
     8881 ggtatccgga ctttctgcca ggagagataa caggcacggg gacgctcctc ggtacgccca
     8941 ttgggcatct cccgcttaat gaacaatccg ccaccatcca gctcgtccag caatcgccct
     9001 atttgcagct ctccggctgt acctttagcg tggaagaaga agttgccttt gggccagaaa
     9061 atctgtgtct tgctgaaaca gagattatgg cgcggattga tgccgccctg actctgaccg
     9121 agtgccagcc gttgcgccat cgtcacccgg caacactctc cggtggagag acacaacgcg
     9181 tggtcatcgc ctgcgccatc gcgatgcagc caaagctctt aattcttgat gaagccttta
     9241 gtcgcctgac gccgcaggcc agcgaaatgc tgttgcagcg cttacaacac tgggcgcttg
     9301 aacgtggttc gctgattatt ctttttgaac gtcatcgcac acctttcctg aactactgcc
     9361 agcaagcgtg gcaattgcaa aacggagcgc tccagccact atgttaacgt taaatcagat
     9421 atcttatcgc tggcctagcg cagcaacgga ttgtctttgc gacatttctc tgcaactcaa
     9481 acaaggtgaa tggttggcgc tgaccggcga caatggcgcg ggaaaatcga cgctgttacg
     9541 agtaatggca ggtcttctta cgcccacagc cggtacggtt atgctgcaac aacaggcgat
     9601 gaaaaacctg aaaaaccgcc agcgcgcggc aaaggttggc gtactgtttc aggaagcgga
     9661 aaaccaactt tttcacagca ccgtcgctga cgagatcgca tttggtttga aactgcaaaa
     9721 atgcccggcg gatgaaatca ctcaacgtac ccatgctgcc ctgcaatgtt gtcagttagc
     9781 agacactgcc aactcgcacc cgttggatct gcatagtgcg cagcgacgga tggtcgccgt
     9841 cgcctgcctg gaagcactct ctccgccact attactgctg gacgagccga gtcgggattt
     9901 tgacgaaaat tggttgagcg tatttgaaag ctggctggag aagtgccgcc agcgaggcac
     9961 gagcgtggta gcaatcagtc atgacgccgc atttacccgg cgtcatttct ctcgtgtggt
    10021 gcgactggaa gatggtgtaa tcaggaacgt taatccacca gacgatattc acccgtagcg
    10081 gtcatcatta aggtcaaatt tgcaggcaga ctctcttcca gcacccggcg aacattcggc
    10141 ccgtcggtac ggtcataaaa ggcttctgca tctgccagcg atacccctcc cgccagcttg
    10201 cgccccacca gacgctcccg caacgcagta gctggcgcgt tgataaagat cgagaaatca
    10261 caaaattgcg caagctggca ccacttctcg tcgtccagca acaaccagtt tccttcgacg
    10321 atgacgagtg gtgcggtaac gtgcaacgca tcttcaacag gatcatgctt ttgtcgatcg
    10381 tactgcggcc acgtacaatc cccttccacc acctggcgca gattttccgc cagtttcgca
    10441 acgtcaaatg tctctggtgc gcctttgaag gggcgcaatt gatgcgcgtc cagccagcta
    10501 ttgtaatggt gaaaaccatc catcgggagc gtctgaatag cgggcagctc cgggtcttgt
    10561 tgcacgagat attcccagaa ggtggttagg gtagatttac ccgtccccgg cggagcgcaa
    10621 agaaaaacca ccgtacgccg ctgcggattt accgtctgta aggccgccag catacgcagc
    10681 aacggtttat gcacgcgttc tatctcttca tcatggtact gcgcttgcac gttcagccca
    10741 ttcaccgtta attcaatttt cacggcgtta attcctttaa ggtggtcgtt actgccagcg
    10801 tcagcgccgt tttgaccatt ccgctaaaac gcgc
//
LOCUS       DAZPEP010000140        10686 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZPEP010000140 DAZPEP010000000
VERSION     DAZPEP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.140"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..784)
                     /locus_tag="ACHUT9_003412"
     CDS             complement(<1..784)
                     /locus_tag="ACHUT9_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1349811.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="ACHUT9_003413"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="ACHUT9_003413"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFR1349812.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="ACHUT9_003414"
     CDS             1860..2747
                     /locus_tag="ACHUT9_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFR1349813.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASVVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="ACHUT9_003415"
     CDS             2780..3790
                     /locus_tag="ACHUT9_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFR1349814.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDVVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="ACHUT9_003416"
     CDS             3860..5293
                     /locus_tag="ACHUT9_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1349815.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="ACHUT9_003417"
     CDS             5392..6741
                     /locus_tag="ACHUT9_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1349816.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGMFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="ACHUT9_003418"
     CDS             6731..7618
                     /locus_tag="ACHUT9_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFR1349817.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUT9_003419"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="ACHUT9_003419"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFR1349818.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="ACHUT9_003420"
     CDS             8352..9224
                     /locus_tag="ACHUT9_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFR1349819.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUT9_003421"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="ACHUT9_003421"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFR1349820.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUT9_003422"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="ACHUT9_003422"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFR1349821.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2530 c         2856 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagc attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgtcgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgttgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatt gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacagggg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta tgttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tctcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAZPEP010000141        10496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZPEP010000141 DAZPEP010000000
VERSION     DAZPEP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.141"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            53..628
                     /gene="ssuE"
                     /locus_tag="ACHUT9_003423"
     CDS             53..628
                     /gene="ssuE"
                     /locus_tag="ACHUT9_003423"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFR1349822.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            621..1580
                     /gene="ssuA"
                     /locus_tag="ACHUT9_003424"
     CDS             621..1580
                     /gene="ssuA"
                     /locus_tag="ACHUT9_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFR1349823.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1577..2724
                     /gene="ssuD"
                     /locus_tag="ACHUT9_003425"
                     /pseudo
     CDS             1577..2724
                     /gene="ssuD"
                     /locus_tag="ACHUT9_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            2736..3527
                     /gene="ssuC"
                     /locus_tag="ACHUT9_003426"
     CDS             2736..3527
                     /gene="ssuC"
                     /locus_tag="ACHUT9_003426"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFR1349824.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3524..4291
                     /gene="ssuB"
                     /locus_tag="ACHUT9_003427"
     CDS             3524..4291
                     /gene="ssuB"
                     /locus_tag="ACHUT9_003427"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFR1349825.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4498..7110)
                     /gene="pepN"
                     /locus_tag="ACHUT9_003428"
     CDS             complement(4498..7110)
                     /gene="pepN"
                     /locus_tag="ACHUT9_003428"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFR1349826.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7376..8578
                     /gene="pncB"
                     /locus_tag="ACHUT9_003429"
     CDS             7376..8578
                     /gene="pncB"
                     /locus_tag="ACHUT9_003429"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFR1349827.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8748..10148
                     /gene="asnS"
                     /locus_tag="ACHUT9_003430"
     CDS             8748..10148
                     /gene="asnS"
                     /locus_tag="ACHUT9_003430"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFR1349828.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2379 a         2691 c         2829 g         2597 t
ORIGIN      
        1 gaattgttat caatgttaac aaaaaaagaa caattggtta taaggagaga gtatgcgtgt
       61 catcaccctg gcgggtagtc ctcgctttcc ttctcgctcc agctccttgc tggaatatgc
      121 gcgggaaaaa ctaaatggcc tggatgtaga ggtttatcac tggaatctgc aaaacttcgc
      181 cccggaagat ctgctttacg ctcgtttcga tagtccggca ctcaagacct tcaccgaaca
      241 gctgcaacag gctgatggac tgatagtcgc tacacctgtg tataaagccg cctattccgg
      301 cgcgctgaaa accctgctcg acctgctgcc agagcgcgct ttgcaaggca aagtggtgct
      361 accgctggcg acgggcggta cggtggctca tctgctggcg gtcgattatg cccttaagcc
      421 agttttaagt gcgatgaaag cccaggagct cctgcacggc gtgtttgccg atgactcaca
      481 agttattgat taccatcaca aaccccagtt cacgccaaat ctgcaaaccc gtcttgatac
      541 cgcgctagaa actttctggc aggcattgca ccgccgcgat gttcaggttc ctgaccttct
      601 gtctctgcga ggtaatgtcc atgcgtaaca tcattaaact ggcgctggcg ggattgctta
      661 gcgtctctac ttttgcggtt gctgcagaat cctcgcctga agcgttacgt ataggctatc
      721 agaaaggcag tattggtatg gtattggcaa aaagccacca gttactggaa aaacgctatc
      781 cgcaatcaaa aatctcctgg gtggagttcc ccgcgggtcc gcaaatgttg gaagcgttaa
      841 acgttggcag tattgatctc ggcagtaccg gggatattcc gccaatcttt gcccaggctg
      901 ccggggctga tttgatatat gtgggcgtcg agccaccgaa gcccaaagcc gaagtgattc
      961 tggtggcaga aaacagcccg atcaaaaccg tagccgatct taaaggtcac aaagttgcct
     1021 ttcagaaagg ttccagttca cacaaccttt tactgcgtgc actgcgtcag gccggactta
     1081 agtttactga catccagccc acttatctga cgccagctga tgcccgcgcc gcgttccagc
     1141 aaggtaacgt tgacgcctgg gctatctggg atccctacta ctccgctgcg ttattacagg
     1201 gcggcgtgcg ggtgctgaaa gacggcaccg atctcaatca aactggatcg ttttatctgg
     1261 cagctcgccc ctatgcagaa aaaaacggcg cttttattca gggcgtactg gcaaccttta
     1321 gtgaggccga tgcgttaacc cgcagccagc gcgagcaaag catcgcttta ctggcaaaaa
     1381 cgatgggctt accggcaccg gtgattgcct cgtatctgga tcatcgtcct cccaccacca
     1441 tcaaaccggt gagcgctgaa gttgccgcct tacagcagca aacggcagat ctgttttatg
     1501 aaaaccgtct ggtgtcgaaa aaagttgata ttcgccagcg catctggcag cccactcacc
     1561 tggaaggaaa acaattatga gtctgaatat gttctggttt ttaccgaccc atggtgacgg
     1621 gcattatctt ggaacggaag aaggttcacg cccggttgat cacggctatc tgcaacaaat
     1681 tgcgcaagcg gcggatcgtc tgggctatac cggtgtgctg atcccaacgg ggcgatcgtg
     1741 tgaagatgcg tggctggtgg cggcgtcgat gatcccggtg acgcagcggc tgaagtttct
     1801 tgtcgccctg cgtcccagcg taacctcacc taccgttgcc gctcgccagg ccgccacgct
     1861 tgaccgtctc tcaaatggtc gtgcgttgtt taatctggtc acaggcagcg atccacaaga
     1921 gttggcaggc gacggcgtgt tccttgatca tagcgagcgc tacgaagcct cggcggaatt
     1981 tacccaggtc tggcggggcg cttattgctg ggggaaagcg tcgatttcaa cggtaaacat
     2041 attcatgtgc ggggagcaaa actgctcttc ccgccgattc aacagccgta tcctccgctt
     2101 tactttggcg gatcgtcaga tgtcgcccag gagctggcgg cagaacaagt tgatctctac
     2161 ctcacctggg gcgaaccgcc agagctggta aaagagaaga tcgaacaagt gcgggcgaaa
     2221 gctgccgcgc atggacgcaa aattcgtttc ggtattcgtc tgcatgtgat tgttcgtgaa
     2281 actaacgacg aagcgtggca ggccgccgag cggttaatct cgcatcttga tgatgaaact
     2341 atcgccaaag cacaggccgc attcgcccgg acggattccg taggacaaca gcgaatggcg
     2401 gcgctacata acggcaagcg cgacaatctg gagatcagcc ccaatttatg ggcgggcgtt
     2461 ggcttagtgc gcggcggtgc cggaacggcg ctggtgggcg atggtcctac ggtcgccgcg
     2521 cgaatcaacg aatacgccgc gcttggcatc gacagctttg tgctttcggg ctacccgcat
     2581 ctggaagaag cgtatcgggt tggcgagttg ctgttcccac atctggatgt cgccattccg
     2641 gaaattcccc agccgcagcc gctgaatccg caaggcgaag cggtggcgaa tgattttatc
     2701 ccccgtaaag tcgcgcaaag ctaaggaaca cgatgatggc aacgccagtg aagaagtggt
     2761 tattgcgcgt tgccccctgg tttttaccgg tgggcatcgt ggcggtgtgg caactggcct
     2821 cctcggttgg ctggctgtcg acgcgtattt tgccttcacc ggaaggagtg gtgacggcgt
     2881 tctggacgct ctccgccagc ggcgaattgt ggcagcatct ggcgatcagt tcctggcggg
     2941 cgctgattgg cttttcaatt ggcggatcgc tgggcttgat tctgggactg attagcgggc
     3001 tgtcacgctg gggagaacgg ctgctggata cctcaattca gatgttgcgc aacgtaccgc
     3061 atctggcgct gatcccgctg gtgattttgt ggtttggcat tgatgaatcc gcaaaaatct
     3121 ttctggtggc gctcggcacg ctattcccca tttatatcaa cacctggcat ggcatccgta
     3181 atatcgatcg cggtctggtg gaaatggcgc gtagctatgg attatccggc ataccgctgt
     3241 ttatccatgt gatcctgcct ggtgccctgc cctcaattat ggtcggcgtg cgttttgcgt
     3301 tgggcctgat gtggctgacg ctgattgttg ccgaaaccat ttctgccaat tcaggcattg
     3361 gttatctggc gatgaatgcg cgggagtttt tgcaaacgga cgtggtggta gtcgctatta
     3421 ttctttacgc cctgctcggc aaacttgctg acgtcagcgc gcagctgtta gagcgcctct
     3481 ggttgcgctg gaacccggct tatcatttga aggaggccac ggtatgaata ctgctcgtct
     3541 gaaccagggc acaccattgt tgctcaatgc agtaagcaaa cattacgcgg aaaatatcgt
     3601 cctgaaccaa ctggatttac atattccggc aggtcagttt gtggcggtgg tgggccgcag
     3661 cggtggtggc aaaagtaccc tgctgcgcct gctggcaggt ctggaaacgc caaccgcagg
     3721 cgatgtgtta gcgggcacca caccgctggc tgagattcag gaagacacgc gaatgatgtt
     3781 tcaggatgcg cgtctgctgc cgtggaaatc ggtgattgat aacgttgggt taggccttaa
     3841 aggccagtgg cgcgatgccg cgcgtcgggc gttggctgca gtaggactgg agaatcgcgc
     3901 cggggaatgg cctgccgcac tttctggcgg gcagaaacag cgtgtggcgc tggcaagagc
     3961 gttgattcat cgaccgggtt tattgttgct tgatgaaccg ctcggggcac tggacgcctt
     4021 aacgcgactc gagatgcagg atttgattgt gtcactttgg cagcagcacg gctttaccgt
     4081 actgttggtg acgcatgatg tgagcgaagc ggtagcaatg gctgaccggg tgttgttaat
     4141 tgaagaggga aaaattggtc tggatttgac agtggatatc ccccgtccgc gtcgattagg
     4201 gtcggtgcga ttagcggagc tggaagcgga agtattgcaa cgggtaatgc ggcgtgggca
     4261 ttctgaacaa ctgattcggc gtcatggtta atatgccgga tgtggcgcac aaaaccagca
     4321 ttttcaacgg acttgtaggc ctgataagac acgtcaagca tcgcatcagg cagttgggca
     4381 cggctgccgg atgcggcgtg aacgccttat ccagcctaca aaaccagcaa tttcaacgga
     4441 gttgtaggcc tgataagacg cgtcaagcgt cgcatcaggc attttggctc aaaagtatca
     4501 agccagtgct ttggtgatct tctcgtacag atcgccagag agattttcca gccctttcaa
     4561 ctgttccagc gccgcgcgca ttttctcctg acgtttggca tcataacgtt tcaggcgaat
     4621 cagcggttca atcagacgtg aagctacctg cgggttacgg ctgttgaggt cggtaagcat
     4681 ttccaccagg aactggtaac cgctgccatc ttcggcatgg aacgctgccg ggttgctgcc
     4741 cgcaaacgca ccaatcaacg aacgaatacg gttcgggttg ctcatggtaa atgagcgatg
     4801 ctgcaacaga ccacgcaccg tctccagcac attcgccgcc gggctggtgg cttgcaggat
     4861 aaaccattta tccatcacca gaccgtcctg atgccactta tcgtcgtact cctgcatcag
     4921 cgcgtcacgg caaggcagct gtgcagcaac cgccgcagaa agcgctgcca gcgcatcggt
     4981 catattgttt gcttcatggt attgcttgct caccagcacg tcagccagat gcgtttcacc
     5041 aaaagcaagg aagcgcaggc aggcatttcg cagagtgcgt tttgcaatat cttcatgctc
     5101 aacacggtac tcgctctggt aattcgcgtt gtaaatagcc agcagctcat ccgccagttc
     5161 agtcgccaga gtacgagtga gtgcttcgcg tacttcggca atagcaatcg ggtcgatgat
     5221 atcgaacagt tcagccattt cattgacaga aggcagcgtc aggatttctg ccgccagcgc
     5281 cggatcaatc ttctcatcga gcagcaccgc gcggaaagcg tcagccacat gtaccggcag
     5341 agacagcggc tgcccttgct gatgacgggc gacgttcagc ttgatgtagg ttgccagcaa
     5401 actttgcgcc gcatcccaac gggagaaatc attacgcgca tgacgcatca ggaaggtcag
     5461 ttgctgatca ctccacttat attccagttt cactggcgca gagaattcgc acagcagcgc
     5521 aggcaccggc tggaagtaga cattatcaaa aacaaaggtc tgttccgcct gggtgacgtt
     5581 cagcacggaa ttcaccggat gaccgccttt ctgcaacggg atcactttgc cttcgttgtc
     5641 atacaattcg atggcaaacg gaatatgcag cggctgtttt tctgcctgat ccggcgtggc
     5701 tggcgtgcgc tggctgatgg tcagggtgta ctgctcagtt tccggattgt agtcgtcttt
     5761 gacggtcaca atcggcgtac cggactggct gtaccaacgg cggaaatggg agagatcaac
     5821 attcgacgca tcttccatcg cctgcacaaa gtcgtcacag gtcgctgcgc taccatcatg
     5881 acgctcgaaa tagagttgca tccctttctg gaagttttct tcgcccagca gggtgtggat
     5941 catacggatc acttccgcgc ccttctcgta aacggtcagg gtgtagaagt tgttcatctc
     6001 aatgaccatg tccgggcgga ttgggtgcgc catcgggctg gcgtcttccg caaactgcaa
     6061 tccgcgcatg gtgcgcacat tattgatgcg gttaactgcg cgggaaccaa ggtcagagct
     6121 gaattcctga tcgcggaaga cggttaaacc ttctttcagg ctgagctgga accagtcgcg
     6181 gcaggtcact cggttaccgg tccagttatg gaaatattca tggccgataa cgcgttcaat
     6241 atcgaggtaa tctttgtcgg tggcggtgtc ggtgcgggcc agcacatatt tggagttaaa
     6301 gatattcaga cccttattct ccattgcgcc catattgaag aaatccaccg cgacgatcat
     6361 atagatgtcg aggtcatact ccaggccgaa gcgttcttca tcccatttca ttgagttttt
     6421 cagcgaggtc atcgcccacg gcgcgcgatc aaggttgccg cgatcgacgt acagctccag
     6481 cgccacttcg cgaccagaac gcgtggtaaa ggtgtcgcgc agtacatcaa agtcgcctgc
     6541 caccagcgca aacaggtagc acggtttcgg gaacgggtcc tgccactgta cccaatggcg
     6601 tccgttttcc agttcacctt gcgcaacgcg gttaccgttg gaaagcagga agggatattt
     6661 ggttttatcg gcaataattt tggtggtaaa acgcgccagc acgtccgggc ggtcgagata
     6721 atacgtaata tggcggaaac cttcggcttc acactgggtg caaagcgcat cgcctgactg
     6781 ataaagccct tccagcgcgg tattcgccgc cgggcttatt tcattaacga tcttaagcgt
     6841 aaaatgctcc ggcaaattac tgatgaccag tgcgccctct tcttctttcc aggcggtcca
     6901 cggctcatca ttaatatgaa cagaaaccag tttgaggtct tcgccgttga gacgaagtgg
     6961 agcatctgat gcaccatgac ggacagcctg gctgaccgcg gtaacgaccg tcttttgcgc
     7021 gtcgaggtca aaggtcaagt caatatcagt aatctggtaa tccggcgcac gataatcgtg
     7081 acggtatttg gcttgtggct gttgagtcat aaataacctt tagcatcttt tatagagtct
     7141 ggtgttcagt ctattcctgt tgcgtaaatc gcgctatgca gaatcttcat cttttcaggt
     7201 acaaacgcct ttattgctac atttttataa catacaccgc gtaatgccat cgaccagaaa
     7261 ggtggcatat ggtgtgatcg gggttcaata aattgcgaaa caaggtatac tccagcagtt
     7321 cctgaagatg tttattgtac taaacgctcc tgtacgagga cgctactgcg cacctatgac
     7381 acaattcgct tctcctgttc tgcactcgtt gctggataca gatgcttata agttgcatat
     7441 gcagcaagcc gtgtttcatc actattacga tgtgcatgtc gcggcggagt ttcgttgccg
     7501 aggtgacgat ctgctgggta tttatgccga tgctattcgt gaacagattc aggcgatgca
     7561 gcacctgcgc ctgcaggatg atgaatatca gtggctctct gccctgcctt tctttaaggc
     7621 cgactatctt aactggttac gcgagttccg ctttaacccg gaacaagtca ccgtgtccaa
     7681 cgataatggc aagctggata ttcgcttaag cggtccgtgg cgtgaagtca tcctctggga
     7741 agttcctttg ctggcggtta tcagtgaaat ggtacatcgc tatcgctcac cgcaggccga
     7801 cgttgcgcaa gccctcgaca cgctggaaag caaattagtc gacttctcgg cgttaaccgc
     7861 cggtcttgat atgtcgcgct tccatctgat ggattttggc acccgtcgcc gtttttctcg
     7921 cgaagtacaa gaaaccatcg ttaagcgtct gcaacaggaa tcctggttcg tgggcaccag
     7981 caactacgat ctggcgcgtc ggctttccct cacgccgatg ggaacacagg cacacgaatg
     8041 gttccaggca catcagcaaa tcagcccgga tctagccaac agccagcgag ctgcacttgc
     8101 tgcctggctg gaagagtatc ccgaccaact tggcattgca ttaaccgact gcatcactat
     8161 ggatgctttc ctgcgtgatt tcggtgtcga gttcgctagt cggtatcagg gcctgcgtca
     8221 tgactctggc gacccggttg aatggggtga aaaagccatt gcacattatg aaaagctggg
     8281 aattgatcca cagagtaaaa cgctggtttt ctctgacaat ctggatttac gcaaagcggt
     8341 tgagctatac cgccacttct cttcccgcgt gcaattaagt tttggtattg ggactcgcct
     8401 gacctgcgat atcccccagg taaaacccct gaatattgtg attaagttgg tagagtgtaa
     8461 cggtaaaccg gtggcgaaac tttctgacag ccctggcaaa actatctgcc atgataaagc
     8521 gtttgttcgg gcgctgcgca aagcgttcga ccttccgcat attaaaaaag ccagttaata
     8581 tcatcaggga gctaatcggc tccctttttt tacctttaat tccgaaatct ttcgctgcat
     8641 ttgcgaattc tgcttgtctg attgcagatg ccaggtaaca taggtatccc ccccatttac
     8701 gggtggacaa gtgtttattt tttccgacta ttaacagaga gaatattatg agcgttgtgc
     8761 ctgtagccga cgtactccag ggccgcgtag ccgttgacag cgaagtcacc gtacgcggat
     8821 gggtacgtac ccgccgagat tcaaaagctg gcatctcctt cctcgccgtt tatgacggtt
     8881 cctgctttga tcctgtacag gctgtcatca ataattctct gcccaattac aatgaagacg
     8941 tcctgcgtct gaccaccggc tgctcggtca ttgtgacggg taaagtcgtg gcatcgccgg
     9001 gccaggggca acaatttgaa attcaggcca gcaaggttga agttgctggt tgggttgaag
     9061 atccagacac ttacccgatg gcggcaaaac gccacagcat tgagtatctg cgtgaagtcg
     9121 ctcacctgcg tccgcgcaca aacctgattg gtgccgtcgc gcgcgttcgc catacgctgg
     9181 cgcaggcgct gcatcgcttc tttaacgagc agggattctt ctgggtttca acgccactga
     9241 ttaccgcatc tgataccgaa ggtgcaggcg aaatgttccg cgtttctacg ctggatctgg
     9301 aaaacctgcc gcgtaacgat cagggcaaag tggatttcga caaagacttc tttggtaaag
     9361 agtctttcct gaccgtatct ggccagttga acggcgaaac ctacgcttgc gcattatcca
     9421 aaatttatac cttcggcccg actttccgtg ctgaaaactc caacaccagc cgtcacctgg
     9481 cggaattctg gatgctggag ccggaagtgg cgtttgctaa cctgaacgat atcgcgggtc
     9541 tggctgaagc catgctgaaa tatgtcttca aagcggttct cgaagaacgc gcagacgaca
     9601 tgaaattctt cgctgaacgc gtagataaag atgccgtttc acgtctggaa cgcttcattg
     9661 aggccgattt cgcgcaggtg gattacaccg acgcagtgac cattctcgaa aactgcggca
     9721 ggaagtttga aaacccggtt tactggggcg tcgatctctc ttctgagcat gagcgttatc
     9781 tggcggaaga acactttaaa gcaccggtag tggttaaaaa ctatccgaaa gatattaaag
     9841 cgttctatat gcgcctcaac gaagacggta aaaccgttgc ggctatggac gttctggctc
     9901 cgggcatcgg tgagatcatt ggtggctccc agcgtgaaga acgtctggac gttctggacg
     9961 agcgtatgct ggaaatgggc ctgaataaag aagattactg gtggtatcgc gatctgcgtc
    10021 gctacggtac tgttccgcat tcaggtttcg gtcttggttt tgaacgcctg attgcttacg
    10081 taactggcgt gcaaaacgtg cgtgatgtga ttccgttccc acgtactccg cgcaacgcca
    10141 gcttctgatt ttcttcctca aagagccagc gcctgctggc tcttttttat cctttctgtc
    10201 aagttatctg tttgttaagt caagcaatct atttgcaacc ccgccataac gatcatcctg
    10261 ttacggaata ttacattgca acatttacgc acaaaaaata acccgcattc ttattgcgga
    10321 ttagtttttt cttagctgat agcacaattt tcatactatt ttttggcatt ctggatgtct
    10381 gaaagaggat tttgtgccag gtcgataaag tttccatcag aaacaaaatt tccgtttagt
    10441 taatttaaat ataagtaaat catataaata gataaaaatt gctgtaaata tcatca
//
LOCUS       DAZPEP010000142        10455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZPEP010000142 DAZPEP010000000
VERSION     DAZPEP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.142"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="ACHUT9_003431"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="ACHUT9_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFR1349829.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUT9_003432"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="ACHUT9_003432"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFR1349830.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUT9_003433"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="ACHUT9_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFR1349831.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUT9_003434"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="ACHUT9_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFR1349832.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUT9_003435"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="ACHUT9_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUT9_003436"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="ACHUT9_003436"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1349833.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUT9_003437"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="ACHUT9_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFR1349834.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUT9_003438"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="ACHUT9_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFR1349835.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="ACHUT9_003439"
     CDS             complement(9457..10149)
                     /locus_tag="ACHUT9_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFR1349836.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2556 a         2522 c         2763 g         2614 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtgggtcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
    10441 cgtattatgc acacc
//
LOCUS       DAZPEP010000143        10215 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZPEP010000143 DAZPEP010000000
VERSION     DAZPEP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10215
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.143"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(54..2006)
                     /gene="betT"
                     /locus_tag="ACHUT9_003440"
                     /pseudo
     CDS             complement(54..2006)
                     /gene="betT"
                     /locus_tag="ACHUT9_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
     gene            2145..2732
                     /gene="betI"
                     /locus_tag="ACHUT9_003441"
     CDS             2145..2732
                     /gene="betI"
                     /locus_tag="ACHUT9_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFR1349837.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2746..4218
                     /gene="betB"
                     /locus_tag="ACHUT9_003442"
     CDS             2746..4218
                     /gene="betB"
                     /locus_tag="ACHUT9_003442"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFR1349838.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4232..5902
                     /gene="betA"
                     /locus_tag="ACHUT9_003443"
     CDS             4232..5902
                     /gene="betA"
                     /locus_tag="ACHUT9_003443"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFR1349839.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPTLDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            6115..6783
                     /gene="ykgH"
                     /locus_tag="ACHUT9_003444"
     CDS             6115..6783
                     /gene="ykgH"
                     /locus_tag="ACHUT9_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFR1349840.1"
                     /translation="MSEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYVNK
                     DNIKVSYSLKINQTTPGILVNCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQIPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(7026..7721)
                     /locus_tag="ACHUT9_003445"
     CDS             complement(7026..7721)
                     /locus_tag="ACHUT9_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFR1349841.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(7714..9141)
                     /locus_tag="ACHUT9_003446"
     CDS             complement(7714..9141)
                     /locus_tag="ACHUT9_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFR1349842.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(9152..9871)
                     /locus_tag="ACHUT9_003447"
     CDS             complement(9152..9871)
                     /locus_tag="ACHUT9_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFR1349843.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         2558 a         2772 c         2551 g         2334 t
ORIGIN      
        1 gtcaatgatt cccgcgccat atgcagacat ggcgcggttt tatgcaaaaa caatcacgcg
       61 tccgggaaca tcacgctatg gcccggcgct tcacgatgga gatgaataaa gttaaggtgc
      121 cgctcgtact ggtcaagaat atcggtgatc acctgctctt tgctgtagtc catcaggtcg
      181 ttgccctggc tgccttctaa caggaaggtt tccagtcggt agtaggtcga tttaccgata
      241 cgtgcgcggt aggtaaagcc cggcaccgaa tattgctgcg gccaaatctg atagacaaag
      301 ttttgctctt cgcccatatg caccaacaaa tccagatgcc ccaactgttg tccctcttcc
      361 ggcggcaggc tttttagctc cacgtacgcg ccgcgcaacc gcagctcctg cgccacttct
      421 tccattgccg ggtaacagac cgtctccatc atctgtttag tgtaacgcgt gcccggataa
      481 ttcatcaggc gcgatagacg ttttttccag ctcaggcgat cctgaagccc cagcggtcgc
      541 ggcgcggtat cgcggttgac actttcacgg cggtaatctt ctaccttcag agatttatac
      601 aaccccgcca tcacgaagaa aatcacaaag ctgaacggca gccccatgat taccgtggtg
      661 ttttgcagcg cggatatccc gttggtcatc agcatgccga gcgtcagcag accaatcgcc
      721 accgaccaga agacgcgcag ccagccgggg gcgtcgctgt tgatatcttt aagctgcgag
      781 gtgaaattcc ccagcaccag cgcaccggag tccgccgagg tcacataaaa cagcaggcca
      841 gtaatggtgg cgacggaggc gctaaaggta aacgccggat actgcgccag caggctgtag
      901 aagccgcgct ccggatggac catcgcttcc tcggcaaatg ccgcgccgcc gtggatgatt
      961 tcatacagcg cgctattgcc gaataccgag agccataaca gcgtgaaggt aaacggaata
     1021 atcaacgtgc ccagcacgaa ctggcgaatg gtacgcccac gcgagatacg cgccaggaac
     1081 aagccgacaa acggcgacca tgccacccac catgcccaga agaagagcgt ccagttattc
     1141 atccactcaa ccggacggtc gaaggcaaaa ctgttgagcg tcatgcccat aaagcgattc
     1201 acatagtcgc caacgttcag caccagcgca ttaagcagga acgaggtgtc gcccataaac
     1261 aatacgaaca ggatcaatcc cagcgccagc gcaacgttaa gttccgataa cacacgaatg
     1321 cccttatcga caccggaggt gacagagatc gtggcgatta tcaccgacaa ggcgatcagt
     1381 gccgcttttg ccgccatcga atcgggaata tcaaacagta cgctcaagcc atagttaagc
     1441 tgcaccacac cgataccgag cgtagtggca ataccgaaga tagtgccgat caccgctgca
     1501 atatccactg agtgacctat cggcccgtta atccgtttac cgaagatcgg gtacagcgcc
     1561 gagcggatgg tgagcggcaa attataacga tagctaaagt atccgagcgc catgcccatc
     1621 agcgcataca tcgaccagcc ggttaagccg tagtgaaaca gcgtccagac catcgcctga
     1681 cgcgcggcct caatcgtctg tcccgcgcct tccggcggct gcatatactg cgttaccggt
     1741 tcggctacgg agaagaacat caggtcgata ccgatcccgc cagcaaacag catcgccgcc
     1801 caactcagca ggctgaattc cggtttggat tgttctggcc cgagcttcac cgaaccaaaa
     1861 cgcgaacaag cgatacagac cacaaagaca atatagagcg ttgccgccag tagatagtac
     1921 caaccgaagg ttttagaaac ccagtccagc gtgcggccaa tccacagggc cgagaagtcg
     1981 cgaaacagga tcgttgtcag ggaaaaggtc tgtcatccag tatccccact gttattgtta
     2041 ccatttaaaa tcaaattcgt aacaattacg acacatttta tattgaacgt ccaatcaata
     2101 accgctttaa tagataaaca acgctgatga atggagtggc gaaaatgccc aaattgggga
     2161 tgcagtcgat ccggcgcaga caactgatcg acgccacact ggaagcaata aatgaagtgg
     2221 gcatgcacga tgcaacgatc gcgcagatcg cccgccgtgc aggcgtttct acggggatca
     2281 tcagccacta tttcagggac aaaaatggtc tgctggaagc aaccatgcgc gatatcacca
     2341 gtcagctgcg tgacgcggtt ttgaatcgat tacatgcact tccgcagggc agtgcagagc
     2401 agcgattaca ggcgattgtt gccggaaact tcgatgaaac tcaggtgagc agtgcggcga
     2461 tgaaagcctg gctggcgttc tgggccagca gtatgcatca gccgatgctc tatcgtttac
     2521 agcaggtcag cagccgccgc ttgctgtcga atctggtgag cgagtttcgc cgcgaattgc
     2581 cgcgcgaaca ggcacaggaa gcgggctacg gccttgccgc gctgattgac ggattatggc
     2641 tgcgcgcggc actaagcggc aaaccgctgg ataaacctct cgcacactcg ctgacccgtc
     2701 actttattac tcagcattta cccaccgatt aaccgaggag acgtgatgtc ccgaatggca
     2761 gaacagcagc tttatataca tggtggttat acctccgcca ccagcggtcg caccttcgag
     2821 accattaacc cggccaacgg taacgtgctg gcgaccgtgc aggccgccgg gcgtgaggat
     2881 gtcgatcgcg ccgtgaagag tgcccaacag gggcaaaaaa tctgggcggc gatgaccgct
     2941 atggagcgct cgcgtattct gcgtcgggcc gttgatattc tgcgtgaacg caatgacgaa
     3001 ctcgcaaaac tggaaaccct cgataccgga aaagcatatt cggaaacctc aaccgtcgat
     3061 atcgttaccg gtgcggacgt gctggagtac tacgccgggc tgatcccggc gctggaaggc
     3121 agccagatcc cgttgcgtga gacgtcattt gtttataccc gccgcgaacc gctgggtgtg
     3181 gtggcgggga ttggcgcatg gaactacccg attcagattg ccctgtggaa atccgccccg
     3241 gcgctggcgg caggcaacgc aatgattttc aaaccgagcg aagtcacccc gcttaccgcg
     3301 ttaaagctgg ctgaaattta cagcgaagcg ggcctgccgg acggcgtatt taacgtgttg
     3361 ccgggggtgg gcgcggagac cgggcaatat ctgaccgagc atccgggcat tgccaaagtg
     3421 tcatttaccg gcggtgtcgc cagcggcaaa aaagtgatgg ctaactcggc ggcctcttcc
     3481 ctgaaagaag tgaccatgga actgggcggt aaatcaccgc tgatcgtttt cgatgatgcg
     3541 gatctcgatc tcgccgccga tatcgccatg atggcaaact tcttcagctc cggtcaggtg
     3601 tgtaccaatg gcacccgcgt cttcgttccg gcgaaatgca aagccgcatt tgagcagaaa
     3661 attctggcgc gcgttgagcg cattcgcgcg ggcgacgttt tcgatccgca aactaacttc
     3721 ggcccgctgg tcagcttccc gcatcgcgat aacgtgctgc gctatatcgc caaaggcaaa
     3781 gaggaaggcg cgcgcgtact gtgcggcggc gatgtactga aaggcgatgg cttcgataac
     3841 ggcgcatggg ttgcaccgac cgtgttcacc gattgccgcg acgaaatgac catcgtgcgc
     3901 gaagagatct tcggtccggt gatgtcgctt ctcacttatg aatctgagga cgaagtgatt
     3961 cgccgcgcca atgataccga ctacggcctg gcggcgggca tcgtgacggc ggacctgaac
     4021 cgcgcgcatc gcgtcattca tcagctggaa gcgggtattt gctggatcaa cacctggggc
     4081 gaatccccgg cagagatgcc cgttggcggc tacaaacact ccggcattgg tcgcgagaac
     4141 ggcgtgatga cgctccagag ttacacccag gtgaagtcca tccaggttga gatggctaaa
     4201 ttccagtcca tattctaacc aggaggttta tttgcaattt gactacatca ttattggtgc
     4261 cggctcagcc ggcaacgttc tcgctacccg tctgactgaa gatccgaata cctccgtgct
     4321 gctgcttgaa gcgggcggcc cggactatcg ctttgacttc cgcacccaga tgcccgcagc
     4381 gctggcgttc ccactacagg gcaaacgcta caactgggcc tatgaaacag agcctgaacc
     4441 gtttatgaat aaccgccgca tggagtgcgg acgcggtaaa ggcctgggtg gatcgtcgct
     4501 gatcaacggc atgtgctaca tccgtggcaa tgcgctggat ctcgataact gggcgcaaga
     4561 acccggtctg gagaactgga gttatctcga ctgcctgccc tactaccgca aggccgagac
     4621 tcgcgatgtg ggcgagaacg actaccacgg tggcgatggc ccggtgagcg tcactacctc
     4681 caaacccggc gtcaatccgc tgtttgaagc gatgattgaa gcgggcgtgc aggcgggcta
     4741 cccgcgcacg gacgatctca acggttatca gcaggaaggt tttggtccga tggatcgcac
     4801 cgtcacgccg cagggccgtc gcgccagtac cgcgcgcggt tatctcgatc aggccaaatc
     4861 gcgccctaac ctgaccattc gtactcacgc tatgaccgat cacatcattt ttgacggcaa
     4921 acgcgcggtg ggcgtcgaat ggctggaagg cgacagcacc atcccaaccc gcgcaacggc
     4981 caacaaagaa gtgctgttat gtgcaggcgc gattgcctca ccgcagatcc tgcaacgctc
     5041 cggcgtcggc aacgctgaac tgctggcgga gtttgatatt ccgctggtgc atgaattacc
     5101 cggcgtcggc gaaaatcttc aggatcatct ggagatgtat ctgcaatatg agtgcaaaga
     5161 accggtttcc ctctaccctg ccctgcagtg gtggaaccag ccgaaaatcg gtgcggagtg
     5221 gctgtttggc ggcactggcg ttggtgccag caaccacttt gaagcaggcg gatttattcg
     5281 cagccgagag gaatttgcgt ggccgaatat tcagtaccat ttcctgccag tagcgattaa
     5341 ctataacggc tcgaatgcgg tgaaggagca cggtttccag tgccacgtcg gctcaatgcg
     5401 ctcgccaagc cgtgggcatg tgcggattaa atcccgcgac ccgcaccagc atccggcaat
     5461 tctgtttaac tacatgtcac acgagcagga ctggcaggag ttccgcgacg caattcgcat
     5521 cacccgggag atcatgcatc aaccgacgct ggatcagtat cgtggccgcg aaatcagccc
     5581 cggcacggaa tgtcagacgg atgagcagct cgatgagttc gtgcgtaatc acgccgaaac
     5641 cgccttccat ccgtgcggta cctgcaaaat gggttacgac gagatgtccg tggttgacgg
     5701 cgaaggccgc gtgcatgggc tggaaggcct gcgcgtggtg gatgcgtcaa ttatgccgca
     5761 gattatcacc gggaatttga acgccacgac aattatgatt ggcgagaaaa tagcggatat
     5821 gattcgtgga caggaagcgc tgccgaggag cacggcggga tattttgtgg caaatgggat
     5881 gccggtgaga gcgaaaaaat gagtcgtgat gtgaactaac gcaggaaccg atcagattga
     5941 gagttaccgt tccagagagg gggaccgaat ccttatataa acactgaggt aactctcatg
     6001 cttcatatcc agtattcatg atgctggttt tttgctgata tttaccccga acattcctga
     6061 tacagactat taatacaacc ttatctgcgg cagattttat tttctggata tattatgagt
     6121 gaacaaatca aacaggatat cgatctgatt gagattttat tttatctgaa gaaaaagatt
     6181 cgtgttatcc tttttattat agctatctgt atggcaatgg tgcttttatt tctgtatgtc
     6241 aataaagata atataaaagt gagttacagc ctaaaaataa accagacaac accaggcata
     6301 cttgttaact gtgatagcaa taataacttt gcctgtcaga ccacaatgac tgaagatgtt
     6361 atacagcgaa ttactacatt ttttcacacc agcccagatg tcaagaacag agaaataaag
     6421 ctggaatggt caggagataa gagagattta ccaactgctg aagcggaaat atctcgcgtg
     6481 caggcgtcta ttatcaaatg gtatgcgtca gaataccata atggcaggca agttctcgat
     6541 gagatacaaa taccttcagc aattaacagt gagctttata caaaaatgat atacctgacc
     6601 aggaactggt cattgtatcc gaacggtgat ggctgtgtaa ctataagctc accagaaata
     6661 aaaaataaat accctgctgc catttgcctg gctctgggat tttttctaag cattgtaatt
     6721 tctgtaatgt tttgccttgt caaaaaaatg gtagatgaat accaacaaaa ctctgggcag
     6781 taataaacag cattctgata gagagtgcct gtataaagct gaagtgacaa tgaccgggaa
     6841 gggaacccgt tttcaggaat ggatgcctgt ctttttcact gtaaatatcc tggcattact
     6901 aatgccagga tatttacaga agagcagcat aaccatgacg gaacggggtt aagtcaggtt
     6961 gtccttaacg ctattttcaa cctcgtatgc cttcttcagg tttatgttca gatctcatat
     7021 ctctctcagc aatcctcaat aatcagatac accgctttca ccggaccatg aaccccgacg
     7081 actttgataa gctcaatatc cgccgttgag ctgggaccac tgatgatgtt aatacatgac
     7141 ggcattcgtt caccggcctg cgctttctga tgcaattttt ctgcgagttg cgctacacgc
     7201 ggcaggattg tgcttttacg caggataaaa agagaagatt ccgggagcag gctcaatgaa
     7261 cgcccgcgct cgacggcgga aaaaagaacc acgcctcccg attcggttaa accatattca
     7321 gcatacacga cacccacttt agcctgctct gcctgcgaga tattctcggc acctttcgcc
     7381 ggatcccaaa caacggcatt gcattcctgc tgcaaacgtt cactaatccc caattcctcc
     7441 agcctcgtgt caccgctaat cacgaccgac tgatctccca gctctttaca cagacgtatt
     7501 gcagcttctg ccgccttcgc ctcgctggtc agctcacagc gcgtcaacat aacatcgctg
     7561 gcaaactgaa taaacgcgtc acagcgctgc tgttggttaa gttgggtaag ccgctcgtta
     7621 gcatagttgt taagcggcgc atcttctgct tgcggttcaa gtcgcagcgg gcgacccagt
     7681 gcctgagcaa cgttattcaa aaattcgctc cgattatcca ttctttttct cctgcgcctg
     7741 atgtttctta aaccaactac ggaaactctc tccgtcagct tcaggaagat cgcgtgcttc
     7801 catccagtcg ctaatcgcgc caaatttgag tggtgttttg ccgccattga taaaccagct
     7861 tgccgcatga gcgccggcca tcatcccgac tttccacaat cctggatgac tattggcata
     7921 agcgaacatt tttatcgccc gttgctctgc ttttgcggtg atcccttttt cagccatcac
     7981 ccgacgatga cgcaaaatca gttttgacag cggaatacgc accggacaca cgctgtcaca
     8041 agcagtgcat aaagagcagg cgtagggtaa atctttaaaa tctttatagc cgccaagtag
     8101 cggagaaatc accgcaccaa ttggccctgg ataaatagag ccatatccat gaccgccaat
     8161 atggcgatat gccggacaag tattcataca agccccgcag cgaatacagc gcagcacatc
     8221 ccgaaattca gaggccagca cctcagaacg cccgttatcg acaataacca gatgaaactc
     8281 ttcaggacca tcaacgtgcc ccgcttcgcg cggtcctgtc agccaggtgt tgtatcccgt
     8341 caaacgtgca ccaacggcac tgcgcgccag catggtgatc aatacatcta cctcggcaaa
     8401 cgtgggggca atacgctcca ttcccatcac tgcaatatgc gttttaggca gcgtggtaca
     8461 cattcgcgca ttaccttcat tggtcactag gcataccgaa cctgtctctg ccaccgcgaa
     8521 attacagccg gtaataccta tttcagcact gaggaaatct tcgcggattt tttgccggat
     8581 gaataaggtc atcgcttcag gcgtttccgg cccctcatag cccagacgtt cgtgtagcac
     8641 tcgacggatc tgatggcgat ctttatgaat tgccgggacc acaacatgag atggcggatc
     8701 ttgatccagc tggagaatat attcacccag atcggtttca atcacctgaa tgccagcatc
     8761 ctgcaacaca tgattgacac caatctcttc ggtcaccatc gatttagatt tcaccacctt
     8821 ccgggcattt ttgcgttggg caacctgtaa aatgtagcgg gtagcgtctt ctttggtttt
     8881 tgcaaaccag acgtgaccgc cgttttgcgt cactttttct gagagctggt acagataagc
     8941 gtcgagatta ctcagaacat gatcacgtat ctgggcggcc cgatcgcgcc actcctccca
     9001 gtgccccaat tcatcgacca ttttttgccg atttgcccca atacgctgct gcgcgtttgc
     9061 caccgctttg cgcatgatcg gatcttcaat ttgctgacgg atgcgtgtct taaaatctgt
     9121 attactggtt ttgatcgaca tctttatatc ctcagcggct catcaacact tcagcaatat
     9181 gcatcacttt gactttctgc ccttcccgtt gtaatcgccc actgatgttc agcaggcaac
     9241 tcacgtcagc accaattaaa tactcagggc ggacttccat caggtgcaca accttttctt
     9301 tcaccatctc gccggatatt tcggccattt tgaccgagaa cgtgccgcca aatccgcagc
     9361 aggtatcctg ttcagcaaag gtcaacagct ccagtccacg cacatttttc agcagcgtaa
     9421 gtggctcgtc cttcactccc agcttacggg ccaggctaca agatgggtga tacacagctc
     9481 tcccttgcaa actggcacct acatcgacta cccctaattt attaacaata aaagaggtga
     9541 gatcctgcat acgcgcggca accttttcgg cacgtgatgc ccattcgggt tcatccgcca
     9601 gatacgtcgg gtaacttttt acggcatagg tgcaagagcc agccggtgaa ataatgggat
     9661 cgtcgttatc ctccagtgcg gcgatcagat ttttcatccc tggaatcgct tctttgatat
     9721 aaccgctatt gatcgcaggc tgaccgcagc atccctgttt ctccgggaaa tttacgcgac
     9781 agccgagttt ttccagtagc agcacggagt ctcgtgccat tcttgatttc agggcgtcac
     9841 caatacaggt gacaaagaaa ttgacattca caactaatac tccattactt catgcccatt
     9901 tatgcccgat ggttcccctc cttccggggg atcaatcatc aataaatatg gcgttctttt
     9961 ttaattggct agcaacatac cacccctcat ttgtgtgaca aatgataatc tttcatccaa
    10021 acttcaaatg caataactaa ttttatttct ttcggcatcg ctcacatttt atggggaaat
    10081 cgacgttcat ttttggcata agaaatccat ttaaaaaggc tacatccctt atacctattc
    10141 agagctactt attcaggggt tattgagata tacactgatg gtgtacaaaa aaatcaccga
    10201 caataatcac gtatc
//
LOCUS       DAZPEP010000144         9957 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZPEP010000144 DAZPEP010000000
VERSION     DAZPEP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.145"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..144)
                     /locus_tag="ACHUT9_003448"
     CDS             complement(<1..144)
                     /locus_tag="ACHUT9_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349844.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFS"
     gene            222..1316
                     /gene="ddlA"
                     /locus_tag="ACHUT9_003449"
     CDS             222..1316
                     /gene="ddlA"
                     /locus_tag="ACHUT9_003449"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFR1349845.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1340..1552)
                     /locus_tag="ACHUT9_003450"
     CDS             complement(1340..1552)
                     /locus_tag="ACHUT9_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFR1349846.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1812..2120
                     /locus_tag="ACHUT9_003451"
     CDS             1812..2120
                     /locus_tag="ACHUT9_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFR1349847.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="ACHUT9_003452"
     CDS             complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="ACHUT9_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFR1349848.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="ACHUT9_003453"
     CDS             complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="ACHUT9_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFR1349849.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4858..6015
                     /gene="ampH"
                     /locus_tag="ACHUT9_003454"
     CDS             4858..6015
                     /gene="ampH"
                     /locus_tag="ACHUT9_003454"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFR1349850.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="ACHUT9_003455"
     CDS             complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="ACHUT9_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFR1349851.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6727..9615)
                     /locus_tag="ACHUT9_003456"
     CDS             complement(6727..9615)
                     /locus_tag="ACHUT9_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFR1349852.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDFIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2601 a         2446 c         2369 g         2541 t
ORIGIN      
        1 agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc cgttcatcat
       61 ttttgtgatc tcgcgcacat ctttgagata acgcactttc ttcaccagta cgttgcacgc
      121 tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg ttttgtcgtg
      181 gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag
      241 gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca
      301 ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag
      361 ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata
      421 ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat gagcatcagc
      481 ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc
      541 acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt
      601 ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc accaaacgtc
      661 tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc
      721 acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt gtaaaaccgg
      781 ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag tacgcaattg
      841 ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc
      901 gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga
      961 tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga
     1021 aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg gcgattgccg
     1081 ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc
     1141 cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta
     1201 tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc acacgtttga
     1261 ttgagctggc gctggagcgt cacgctgcgg ataacgcatt gaaaaccaca atgtaatatt
     1321 ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa
     1381 tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc
     1441 aaccttttgc ttcaaatccc agtaacccca ccacgccatt aagaatgaat ataaggccaa
     1501 tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc atggcagctc
     1561 cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca
     1621 ttttcgtaat tgttaataaa tttaacttaa ggttgccatt ttgtaactca caacgaagat
     1681 tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg acaatctaaa
     1741 tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc
     1801 cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca
     1861 atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg
     1921 ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc
     1981 aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg
     2041 ggctgatccc gtttttagta ggctgcctca tttttgcagt ggtggcgcta tggctgcact
     2101 ggcgacatcg ccgccagtaa gatttacgtt gccagatacg gcctaaaacg ccttatctgg
     2161 catgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt
     2221 cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt gaatgccagg
     2281 caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat aaactttctt
     2341 gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac ggcgaatttc
     2401 gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg
     2461 ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt
     2521 actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc
     2581 aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt aagtaccaaa
     2641 ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca ttttccacgg
     2701 ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg
     2761 caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga tcagatcgtc
     2821 aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc tataactttt
     2881 gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg
     2941 ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa cggccggatc
     3001 tgcctgataa tttgactcct gctgcgccac cgccagcacc gagcagacgt tttccagcgt
     3061 cggtgccagc ccctgacttt caaaggtgat ggcgagatct ttcgcccagg catcgcggtc
     3121 tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg ctttttcgcc
     3181 ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa ccgccaacaa
     3241 tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc tcaaggtatg
     3301 ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca gacgtttgta
     3361 gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg
     3421 aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg taccggcaac
     3481 aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat agagaatgcg
     3541 ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc gtacggcgct
     3601 aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag
     3661 ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg gcagtttgat
     3721 ccctaccact gccagccatc cggtccccat cagcgaccag acgatagcgg caatcaccag
     3781 accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga gcgttaccag
     3841 caccggcagg aaggcgatca acgtcatgat ggcgttgata aaactgacgc ccatattctc
     3901 cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac cttcgatatg
     3961 acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac
     4021 atagtgactg acaaagaagt tgttcagtac actgatcacc accgcgatca gcgcaatccc
     4081 cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg gcgaacttag
     4141 cgcggtttga atcaaatcat agaacggtgc ataccaggcg ttgacggcga ccccgacttc
     4201 caccaaaaac caggtgacga agatgatcag tgcagtaccg agtattgacc agtattgcca
     4261 acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta
     4321 agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc
     4381 ggaagcgccg gtgatacgcg ccacccagtc tcccccaccg gcttgccaga agataacggc
     4441 aatcaatgcc caaacaaagg ccgagagaaa aaacgctccc ggctttggga aaaaagactt
     4501 aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat
     4561 gaccactaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc aaggcgctta
     4621 gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa gattgtttac
     4681 ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc
     4741 gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc
     4801 atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac accaccgttg
     4861 aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg
     4921 gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta tgccgatcat
     4981 attttttacg gcagcggtgc aacggggatg gcgctggttg ttatcgacgg taaccagcgc
     5041 gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca gctggattcg
     5101 gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt gaaattgctc
     5161 gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc
     5221 gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca taccagcgcc
     5281 ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc
     5341 gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg
     5401 gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc gtccggcaag
     5461 ccttataccc agttgtttga agagcagatt acccgtccat tggggatgaa agacaccacc
     5521 tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg
     5581 tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc tggcgatatg
     5641 atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggcagat
     5701 cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg catggatgtc
     5761 cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt
     5821 ccggggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat ggcgatgatc
     5881 ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa
     5941 aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa accgttggtt
     6001 atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt
     6061 attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga cgacagaacg
     6121 cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc gcaattcttc
     6181 attccagtca atcattgata ataatgttgc gcggatttgt tcgtaggaat tatgcccaat
     6241 gagctgcacg tcgcgtaatt ccagaattct attttgccag gctaaccagt aaaaagcgtc
     6301 tcgccagagt tgattttgtt caataagcgt aatggtttgt ttggctggta gatgatatcc
     6361 cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc
     6421 cagccccata atataagggg cctgggtaat accgataagt acgttttctt ctctacgcag
     6481 agagataact ccctctaaaa taacgaaagt atcttcgtta ttgacatcac tggaaaatat
     6541 aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac atttatcgag
     6601 cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct ttgcagtaat
     6661 gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc ttcggagtga
     6721 agaatattac caggtatatt taacacccac gttcgcggac cagtcttgat ctacgtcacc
     6781 accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt
     6841 acccagacca acacgtaccg cagacccttc agtaccgtta tcgatgaaat caccgttcac
     6901 atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc
     6961 ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct
     7021 gtcgtaagac tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat caccagactg
     7081 gaacagacca gaaacgctgc cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc
     7141 gtaaccggct ttcaagccga agccccaagc gtcggagttg gtgctaccgt caacgtaagt
     7201 accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca agctaccatc
     7261 aacaaagacg ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctgtcttg
     7321 atctacctga ccagaacggt cattcatgtc acctttagcg aagcctgcag ccgcaccgac
     7381 gatccactta gcgttgttac cgtcaacttt ggtatcaaca ccgaccatga tgccgttaac
     7441 atcctgatca tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac
     7501 ccatgcgccg ccgttatcag ccaggccatg acgagagttg gtcagacgag taccaacggt
     7561 gtcttgttcc aggttccaga tattggtgtt cgcagacggg atgctcagcg ccatgttagc
     7621 gtagtcggtc agctccatct gttgcagaac aacggtgtta ccgcgctgtt cagcctgata
     7681 ggtgtatgca cccaggtcag ctttgttagc agcagagaag gtcgcgttgg ttttggtatc
     7741 atcgatatag atgagctgct tacctgcgta atcagcaatc gcaccagcgc cagtggagtt
     7801 gtcgatacga actttgtagt taccggttgc agctacaacg ttgtctgcag agctgttgtc
     7861 aacttcgtcg ccattgttaa tgtcgttgtt accattaacg gtcaagtgac catcagagtt
     7921 catcgcaata acaccataac cgtagttagc tttgctggtg tcgttggtac gatcgttgac
     7981 gaggtctgca ttcaggatgt aatcattact atgaatgtca aacacacctg cgtaaacatt
     8041 accgttgtta tctttaacgt tgccactggt cagttccaga gtatcagttg caaatacgct
     8101 ggagatgtca ttcatgttgg tgtttacatc cagcacacca ccgttagtaa cggtaatggt
     8161 attggcatac agcggtgcat atgcccaagc tgaagcagta tcttcaccga agttaacggt
     8221 tgagaaggta tcaatggtca ggtgatcggt gttaacttca ccgttaactt ccattgaaga
     8281 accaccgttc agagtgatgg tgtcagaagt cagagaagaa tctgcactca cgttaacctg
     8341 agaaccgctg ttgatcgcca gggtatcgat cagggaagtt ttcgtggttt cccaatcaga
     8401 accgttgttc agaacaacat cgaatacgcc gctctggaat gcgttagcgt tatcaacacg
     8461 attccagcca tcttcagagt aagaagcgta cgggttcagg ctgtttggtg caacgtcgta
     8521 gaaggcttcg tcagcaacaa caacgtcata atcaatattc gctgaaccaa cccatttggt
     8581 gttgttcaga gtcaggttca ggtgatcgac gttctgatcg tcagttgccc aaccgccatg
     8641 agtgtaagca gtggtagagt tgtcaatcag gtgacccgtc gcgtcaaagt tagcgttcca
     8701 ggtactgtca aatacaacgt caccaagcaa ctgagagttg ttgaacgtaa cgttgttgac
     8761 catacgataa tcagaagtgt catcatcgat gaaggctaac gcaacatcat cagcaggaga
     8821 tgcgccattg ccgtagtcac ttggttcaac agaattgccg aaatgaccac gatctgattg
     8881 ttcgtcttca gtccatgaac cagaagttac agtagagtca ttaacaacga tgttgttgtc
     8941 ccactgatga ccttcggtat tgtaggtcgc attaccgagg tcgtgttgaa ccaaagtaat
     9001 acctgcaaca tgggagttgt tgctgatgtt gatgttactt tcaacatcca gagtcacagc
     9061 ggtaccaaga ccagcggtta ccaaacgatc ataatctgtc ttagatgttt taccgtctgc
     9121 atccagataa gtatcggtga agtagaaggc gtcaaaatca tcgtcaatag tagaattagc
     9181 gatgttcaga gtgaagatgt cgccatcaac ccagtcgtgg ccagtataat cgctaccgtt
     9241 ggtccagacg tagtcgccat aaccaacctg gtaagatgta accataccat gaacaattga
     9301 gttggagatg ttcagtgtat taccggtatc tgtgccataa tcgtggttca gataataagt
     9361 agaaattaca ccatttacag tgtcgccgct gaaagtggta tcaaagtaac catcagctgc
     9421 gtggtcatat acataaccac cgtaagtcag ataatcgtga tcaattttag tgctgtcacc
     9481 ccaattgtag atggtggctg cgtcatcgtg atacccgaag gtgttataag tagctgcatt
     9541 agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta caagtttctt
     9601 tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc catcaattat
     9661 caatgcatag aaaacatatt atgtttcgcg ctaacgatta tgctgcgtta cagacgaatg
     9721 gttcaattat tctttcccct aagtccgaat gtggacatat ataatttatg gagaaaaaca
     9781 cgcatcaaat tgataaaagg acgatatttc aacatgttgc agataatgtt atttttgata
     9841 catacgcgtt ataaatatca aaaacacaaa ccagataaat atcttatcaa tatacaacaa
     9901 agtttgaatt attcaattta tagcagcaca accaaacgcc aaaaaaataa taaccaa
//
LOCUS       DAZPEP010000145         9940 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZPEP010000145 DAZPEP010000000
VERSION     DAZPEP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.146"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(184..259)
                     /locus_tag="ACHUT9_003457"
     tRNA            complement(184..259)
                     /locus_tag="ACHUT9_003457"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Lys,seq:ttt)
     gene            complement(263..338)
                     /locus_tag="ACHUT9_003458"
     tRNA            complement(263..338)
                     /locus_tag="ACHUT9_003458"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(303..305),aa:Val,seq:tac)
     gene            complement(488..563)
                     /locus_tag="ACHUT9_003459"
     tRNA            complement(488..563)
                     /locus_tag="ACHUT9_003459"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(528..530),aa:Lys,seq:ttt)
     gene            complement(728..1519)
                     /gene="cpoB"
                     /locus_tag="ACHUT9_003460"
     CDS             complement(728..1519)
                     /gene="cpoB"
                     /locus_tag="ACHUT9_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFR1349853.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(1529..2050)
                     /gene="pal"
                     /locus_tag="ACHUT9_003461"
     CDS             complement(1529..2050)
                     /gene="pal"
                     /locus_tag="ACHUT9_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFR1349854.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(2085..3377)
                     /gene="tolB"
                     /locus_tag="ACHUT9_003462"
     CDS             complement(2085..3377)
                     /gene="tolB"
                     /locus_tag="ACHUT9_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFR1349855.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(3510..4742)
                     /gene="tolA"
                     /locus_tag="ACHUT9_003463"
     CDS             complement(3510..4742)
                     /gene="tolA"
                     /locus_tag="ACHUT9_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFR1349856.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            complement(4807..5235)
                     /gene="tolR"
                     /locus_tag="ACHUT9_003464"
     CDS             complement(4807..5235)
                     /gene="tolR"
                     /locus_tag="ACHUT9_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFR1349857.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(5239..5931)
                     /gene="tolQ"
                     /locus_tag="ACHUT9_003465"
     CDS             complement(5239..5931)
                     /gene="tolQ"
                     /locus_tag="ACHUT9_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFR1349858.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(5928..6332)
                     /gene="ybgC"
                     /locus_tag="ACHUT9_003466"
     CDS             complement(5928..6332)
                     /gene="ybgC"
                     /locus_tag="ACHUT9_003466"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFR1349859.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(6482..6775)
                     /gene="ybgE"
                     /locus_tag="ACHUT9_003467"
     CDS             complement(6482..6775)
                     /gene="ybgE"
                     /locus_tag="ACHUT9_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFR1349860.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(6775..6888)
                     /gene="cydX"
                     /locus_tag="ACHUT9_003468"
     CDS             complement(6775..6888)
                     /gene="cydX"
                     /locus_tag="ACHUT9_003468"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFR1349861.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(6903..8042)
                     /gene="cydB"
                     /locus_tag="ACHUT9_003469"
     CDS             complement(6903..8042)
                     /gene="cydB"
                     /locus_tag="ACHUT9_003469"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFR1349862.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(8058..9626)
                     /gene="cydA"
                     /locus_tag="ACHUT9_003470"
     CDS             complement(8058..9626)
                     /gene="cydA"
                     /locus_tag="ACHUT9_003470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFR1349863.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         2433 a         2698 c         2387 g         2422 t
ORIGIN      
        1 taaaagtcaa ctgctctacc aactgagcta acgacccata cgggtgttac ctgaaagatt
       61 tctactcggg gccaatcttt aagattggtg ggtcgtgcag gatgactcgc ttcgcttgcc
      121 ctacgggccg ttgctggcgc aacgttatcc ttcacgctta acatctgagt ttgatgttaa
      181 aattggtggg tcgtgcagga ttcgaacctg cgaccaattg attaaaagtc aactgctcta
      241 ccaactgagc taacgacccg agtggtgggt gatgacggga tcgaaccgcc gaccccctcc
      301 ttgtaaggga ggtgctctcc cagctgagct aatcacccga tactgcgctg gatactacaa
      361 actacttcca ccgaacccac cgtaattagt ggtgggtcgt gcaggatgac tcgcttcgct
      421 cgccctacgg gccgttgctg acgcaacgtt atcctacacg cttaacatct gtcttcgatg
      481 ctaaaactgg tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc
      541 tctaccaact gagctaacga cccacttttt cgttgctttc ggtttgtttg atatcccgtg
      601 gcaacggcgg catatattac tgatttcaga cttgagcgca acaaaaattt cgatgcagat
      661 cactcaactg cttatgattc gcacgacacg accagaaata atgcgacttc tggtcgtgtg
      721 ttatgcatta catcgcgttc agacgttttt gtgcctgttt agcgccatca gtaccagggt
      781 atttactgat aacctgctgg tatacggctt tcgcttttgc ggtgtcacct ttgtcctgca
      841 tgatgacgcc gactttaaac atcgcatcag cagcctttgg tgacttcgga tagtttttca
      901 ctaccgaagc aaaatagtac gccgcatcat cttttttacc cttgttgtag tttaactgac
      961 ccagccaata attggcgttt ggcaggtaag ttgaatcagg gtaatttttg atgaaattct
     1021 gaaatgccac cattgcgtca tcctggcggg atttatcctg caccagcgca atagctgcat
     1081 tgtaatccgt gtttgcatcg ccgcttttca ccggcgcgcc agcattcgca gtaccagcat
     1141 cagctgtcgg cgtcgttgat gccgccgcac cgctttgatc gctgctggtt gattgcgccg
     1201 ctgcaccacc gctgctgagg ctgtcgatct gcaacaggat ctgcttttgc cgctccacga
     1261 cctgattcag ttgatactga ttttcctgaa tctgaccacg cagggaatca atatcggatt
     1321 gattatcaga aagttgttgc tggagttggg ttaaaagctg gctgtgagca ttagaaatac
     1381 gctcaagttg agtgacgcgg tcttcgaccg agcctgagcc gacactactg attggtgcct
     1441 gagcaaaagc ggcccagggg gccgctatac caaccagtaa cgacagactc aatagttgat
     1501 gtctgaagtt actgctcatg caattctctt agtaaaccag taccgcacga cggtttttgg
     1561 agtatgccgc ttcgtcatga cccagtactg caggtttttc tttaccgtaa gaaacgatgg
     1621 agatctggtc tgcagaaacg cctttaccct gcaggtacat cttaacggcg ttcgcacgac
     1681 gttcacccag ggagatgttg tattccggag taccacgttc gtccgcgtga ccttctacgg
     1741 tgactttgta agacgggttg ctacgcagga agtttgcatg tgcatccagc atttgagcga
     1801 agtcagaacg gatatcgtac ttgtccagat cgaagtaaac gatgttgttc tgctgcagct
     1861 gttgcatttg cagacgagcc tgctcttcgg aagacatgtt gccgttgccg ccgttcgcat
     1921 ccataccagt gccggcaccc agcatgcctt cgctgccgtc attgctggcg ttcttgttgg
     1981 aagaacatgc cgcaattgcc ataacaggca gagcaatcat cagccctttc agcactttgt
     2041 tcagttgcat ttcaatgatt cctttactat tcaattaatt attatcacag atacggcgac
     2101 caggcaggga atttgacctg tccatcagtt gccggaagac gcgctttgaa acgcccatct
     2161 gtagaaacca aattcagcac ggatcccatc ccctgagaag agctgtagat taccatagtg
     2221 ccgttaggtg ccagacttgg cgtttcatcc aggaacgtgg acgacagaac ttgtacgcct
     2281 cccgttgcca gatcttgttt ggcaatgtgc tgctgcccac cgttggagct gaccattacc
     2341 ataaatttac cgtcgctgct gacatccgca tcctggttct gcgaaccttc ccaggtaata
     2401 cgttgtggcg caccgccgtt gatattcact ttataaacct gtggacgacc ggcctggtca
     2461 gaagtaaatg ccaggttctg gctgtccggg aaccaggtcg gttcggtatt gttactgcga
     2521 ccatcagtca cctggcggat ctgaccagaa gccaaatcca ttacgtacag gttcaggcta
     2581 ccggttttcg acaaggcgaa tgccagtttg ctgccgtctg gcgagaatgc aggcgcaccg
     2641 ttgtgacgcg ggaatgaagc cacctgacgt acagcgccgt ttgccagcgt ttggataact
     2701 agcgcggaac gaccgctttc gaaggtcaca taagccagtt tagaaccgtc tggtgaccac
     2761 gccggggaca tcagcggctg tggtgaacgg tgaacgacaa actggttgta accgtcatag
     2821 tcagatacgc gcagttcata cgggaactga ccgccgttgg tctgaacaac gtaggcaata
     2881 cgggtacgga acgcaccttt aatgccggtc agtttttcaa acacttcatc actggcggta
     2941 tgaccagcat agcgcagcca ctgcttgttc actttgtacg agttctgagc aagtacagta
     3001 cccggtgcgc cgccagtgtc aacaagttga taagcaacat tgtaagaacc atccggattc
     3061 ggagtgacct gaccgacaac tacagcgtca atacccagcg cggaccatgc agctggttgt
     3121 acttcctgcg cactacccgg ctgctgtggc agacgagcgc gatctaacgg attaaattta
     3181 ccgctgttac gcaagtcggc agcaacgatg ccgccaatat cttcaggtgc cgcaccaggg
     3241 cccgcccact ggaaaggaac aacaccaata ggacgaccgg aatctacacc gctgtcgatc
     3301 acaatgcgga cttcagcatg cagaactgat gcccacagta tgagaaaacc aaatgctact
     3361 cgtaatgcct gcttcatcat atctccctta tctggaccga tggcccacga taatttagca
     3421 gaatgttaac aaactaaaat acacaaaact accagaaccc cgtggcaacc ggtgcctgat
     3481 gttgaccgtc ggaacagtca acatcgcgat tacggtttga agtccaatgg cgcgtttttg
     3541 aacacttcat atactgcctg gcttggtggt ttcgggatct tcgcaagttt agctgctgcc
     3601 aacgcagcct gacaaagtgc gggatcgcca ccttcaggtt tgatatccag taacatacca
     3661 tcgggtgcca gttttatgcg cagcgtacag gttttgcctg cataggacga tgcgtcatag
     3721 aacttacttt cgatagcaga tttaatctgc ccggcatagt tattgatatc ggcccctgat
     3781 gcgccattgt ttttagtatt accactcccg gcaggcgaag cattgttccc tttcgcccct
     3841 ccccccgttt tcggtgcatt cttaccagag cttagctcac cgaaaatatc atctgcctct
     3901 gcggcagctt ttgctgcagc ggctttttca gctgctgctt ttgccgctgc cgcttttttg
     3961 tctgcggcgg ctttttctgc cgctgctttt gccgctgccg cttttttgtc tgcggcggct
     4021 ttttctgccg ctgctttctt atcagctgct gccttatcag cagccgcttt ttctgctgct
     4081 tttttgtcgg ctgcagcttt ctctgctgcc gctttctttc ttgcttcagc tgcagcagct
     4141 tctgccgctt ccgctttctt cttcagtgcc gcagcggctg cctcggcttt tttctgcgct
     4201 tcggctgcgg ctttggcggc ttctgcttct gcttttttct tcgcgtctgc cgccgctttc
     4261 ttcgctgctt cttccgcagc tttagcatcc gcttcggcct tcgctttagc atctgccgcc
     4321 gctttcgctg ccgcctcttc cgcttgcttc tgctttaact cggcctgttt tgcggcttct
     4381 tcagcctgct ttttctgttc ctgagcagct aaccgctctt tctcaagttg cttcaggcgt
     4441 tcctgttcag ccgcttgttt ctcacgcagt tcttcagcag cctgctgttc ctttatcttg
     4501 cgctgctcat cagaacgctt cgcgcttgat tcctggcttt gcatgcgttt gtactgctca
     4561 actaccgcac ctgaatcaac catgacagcg tcgatggacg aaccaccgcc gcctccggct
     4621 gaagcttcta tattctcatc gaacgaactc cagagcagcg ccgcaaataa gatgacatgc
     4681 agcactgctg aaataattat cgcccgcttg agcttgtcgt tttgttcggt tgcctttgac
     4741 actctcggtt tccaaaaact gttcgcctgt tacccgctct ctttcaagca agggaaacgc
     4801 agatgtttag ataggctgcg tcattaaacc aaccgatttc acacccgcac tatgtaacaa
     4861 gttcagtgct ttaattattt catcgtaagg cacatctttt gcgccaccga tcagaaagac
     4921 cgttttcggg ttggccttga aacggctgga cacttccgcc accacctgct ctggtggtaa
     4981 acgctccagg cgatctttct caaccaccac ggtgtactga ccaataccag acacttcaac
     5041 aatcactggc ggattatcgt tactgctcac cgcctgtgat tcagtagcgt ctggcagatc
     5101 gacctccacg ctctgggtga tgatgggcgc tgtcgccata aagatcagca acagcaccag
     5161 cagtacgtcc agcaacggta caatgttgat ttcggacttg agatcgcgac gacctcgtcc
     5221 acgcgctctg gccatggctt accccttgtt gctctcgcta acggtaaacg cctggcggtg
     5281 cagaatcgcg gtaaactctt ccataaagtt gtcgtaattc agttccagtt tgtttacgcg
     5341 ctggttgagg cggttataag ccattaccgc cggaattgcg gcgaacagac caattgcagt
     5401 cgcaatcaac gcttctgcga taccgggcgc aaccatttgc agtgttgctt gttttaccgc
     5461 cccgagggcg ataaaggcgt gcatgatccc ccagaccgta ccaaacagac caatatacgg
     5521 gctgatggag ccaaccgtac cgaggaacgg aatgtgcgtt tccagatttt caagttcacg
     5581 gttcatggag atacgcatag cacgcgacgc cccttccact acggcttccg gcgcatggct
     5641 attggcacga tgcaggcgca caaactcttt gaacccgctg taaaatattt gttccgaacc
     5701 agtcagatta tcccgtttcc cctgactctc ttgatagagg cgagagagtt cgattccaga
     5761 ccagaattta tcttcaaacg cttcggcttc gcgcgccgct gcgttaagaa tacgggtccg
     5821 ctggataata atggcccagg atgcgattga aaaaccaatc aaaatcaaca tgataagttt
     5881 aaccagaagg ctagccttca ggaacaaatc aaggatattc atgtcagtca ctgcttaaac
     5941 tccgcgacaa tagacttggg aagcgcacga ggcttcattt tgagtgggtc aacgcaaaca
     6001 accagaacct ctgcttcatt cagcaaagta ttctcggcgt tgacaatacg ttgcgtgaaa
     6061 accaaagagg tgccacgcat tgatgttatt tcagtctgta tttcgagcat atcgtcgagc
     6121 cgcgcaggtg cgtaatattc caccgtcatt ttacgtacca caaaggcaac gcgttcagcc
     6181 atcagcgcct gctgactgaa gtgatgatga cgcagcatct ctgtgcgtgc tctttcataa
     6241 aaagcgacgt aactggcgtg gtacaccaca ccaccggcat cggtatcttc atagtagacg
     6301 cgaaccggcc atcgaaacag cgttgtattc actttacatc ccggtaatgc aacaaaagtt
     6361 agagctttta aacgctgcta ctatacgcgc gcgattgagg tttgggaatg agggcaagtt
     6421 aagggagcgt aaaaatttat gggcctggag cggcccataa ttttttaaag agaattctga
     6481 tttaaaagaa gaagaaaatc agcccgacaa tgagaacaat atcggcaagc aatgggcaaa
     6541 aaatcccttg ccaaagaacc ttctgcggac gaaagcccac gccgtgaatc acaccagcac
     6601 agacggccca catcagcaat aaaccatgcc agatttccag ttcactggtc ttcgcggcaa
     6661 aacgagatgg gtcccaaaac atacatcctg ctaacagaag cgccatcacg aaggaaagcg
     6721 cccgcagggg gcgcttgtcc attaccgcat acaaggtcgc gataatctta ctcatcagat
     6781 gtcttcttga ccggctttgc ctgattcgac gtgttcaagc gccagcgcgg tgattacccc
     6841 aaacgaacag gcaagaagcg ttcccagaat ccatgcgaaa taccacattt ttagctcctt
     6901 acttagtaca gagagtgggt gttacgttca atatcttctt tggtgatacg accgaacatt
     6961 ttccagtaac accaagcggt gtagagcaga atgatcggta ccagaaccac cgcaacccaa
     7021 gtcatgacgt taagcgtcag ctggctggaa gttgcatccc acattgtcag acttgcgttc
     7081 atcatggtgc tggacggcat cacaaacggg aacattgcga taccggctgt caggatgatg
     7141 caggccagcg tcagggagga gaacacaaac gcccacgcgg ctttatccat acgtgcagtc
     7201 aggatggtca gcagcggaag aaccacaccc agtgccggaa tagcccacag aattggcgtg
     7261 ttgttgaagt taaccagcca tgcgccagct tcacgaacca cttctttatt cagtgggtta
     7321 gaggctgcgt aatggtccat tgtcgatttc acgacataac catcgatacc gtacatcacc
     7381 catacgccag ccagtgcgaa acagaccagt gtcaccagcg cagccacctg agccgttgca
     7441 cgggtacgca ggtgcagttc gcccacggta cgcatttgca gataggttgc gccctgagta
     7501 atgatcatcc ctacgctcac cacgcctgcc agcaggccga acgggttaag caactggaag
     7561 aagttaccgg tgtagtacag acgcagatat tcatcaacgt tgaacggtac gccctgcaac
     7621 aggttaccga acgctacacc aattaccagc ggcggaacga aactaccaat gaagatgccc
     7681 cagtcccaca tgttacgcca gcgggtttct tcaatcttgg agcggtagtc aaaaccgacc
     7741 ggacggaaga acaaagacgc cagcacgagg atcatcgcca catagaagcc ggagaacgca
     7801 gcggcataga ccatcggcca ggcagcaaag agtgcgccgc ccgcggtgat cagccaaacc
     7861 tggttaccgt cccagtgtgg tgcaatggag ttaatcataa ttcgacgctc ggtgtcgtta
     7921 cgaccgagga aacgggtgag catgcccacc cccatgtcga aaccgtcagt gactgcaaaa
     7981 ccaatcagca gaacgccaac cagcagccac cagataaaac gcaatacttc ataatcgatc
     8041 atttgacgac tcctgtctta gcgtgccggc tgagtagtcg tggaagactg ctcaaagtga
     8101 tagcgaccgg ttttcaggct gcttgggccg aggcgtgcaa acttgaacat taagaacaat
     8161 tctgccacca ggaacagggt atacaggccg caaatcagca ccattgagaa gatgagatcg
     8221 cctgcggtca gtgacgagtt cgccacagct gtcggcagca cttcaccgat agcccacggt
     8281 tggcggccat attcagccac gaaccagccc gcttctacag caatccacgg cagcggaata
     8341 ccgtacagcg cggcgcgcag aagccatttt ttctcgccaa tgcggttgcg gatgacactc
     8401 cagaaagaga gcgcgatgat tgccagaagc aggaagccac acgccaccat gatacggaac
     8461 gcaaagtaca gcggcgctac acgcgggatg gagtctttgg ttgcctgttg aatctgcgct
     8521 tcagtcgcat cagccacgtt tggcgtatag cgtttcagca gcagaccgta accgaggtct
     8581 ttcttcatgc tattgaactg gtcacgaacc gcctggtcgg tagaaccaga acgcagttgt
     8641 tcgagcagag agtacgcctt catcccgtta cgaatgcgtt cttcatgctg caccatcagc
     8701 tctttcaggc cgataaccgg ggtatccacg gaacgcgttg caatgatgcc cagtgcgtaa
     8761 gggatctgaa tcgcaaattt gttcgtctcc tcttcctgat caggaatgcc gaacagagta
     8821 aaggcagcag gcgcaggttg cgtttcccac tcggcttcaa tagcagccag tttggttttc
     8881 tgcacgtcgc ccatttcgta gccggattca tcacccagaa caataacaga cagaacagca
     8941 gccataccga agctggcagc gatagcaaag gagcgtttag cgaaggcgaa gtcacgacct
     9001 ttcagcatat accatgcgct gataccgagg atgaacatcg cgccagtcac ataaccagac
     9061 gctacagtgt gaacgaattt cacctgagca accgggttaa gcaccagctc ggagaagctc
     9121 accatctcca tacgcatagt ttcaaagttg aaatcggacg cgattgggtt ttgcatccag
     9181 ccgttcgcaa ccagaatcca cagtgcggac aggtttgaac cgagcgccac cagccaggtg
     9241 acacacatat gctgaacttt acccagacga tcccaaccga agaagaacag acctacaaag
     9301 gtggattcga ggaagaaggc catcagacct tcgattgcca gcggcgcacc gaagatatcc
     9361 cctacatagt gggaatagta agaccagtta gtcccgaact ggaactccat ggtcagaccg
     9421 gtagccacac ccagagcgaa gttgatacca aacaacttgc cccagaactt ggtcatatct
     9481 ttataaatct gtttgccgga gaggacgtag accgtttcca taatggccag caggaacgcc
     9541 ataccgagcg tcagtggcac aaaaaggaag tggtacatcg cggtcaaggc aaactgtaag
     9601 cgcgacagtt cgactatatc taacatcatg actccttgct catcgcatga agactccgag
     9661 agtgaacccc gttagaaagg gtcacacgca tgccccaata gaaatttgtt tgaacacttt
     9721 catcatacca tgtgaatgca cacaaaaacg gcgtaaagaa aggttgcaaa catgttaata
     9781 aaaactcaaa ttgatcccac gtatatatta cgccgcaaaa tccttacaat aaacaggttt
     9841 ttgttgaagc aaatttgcat ttttcgacgg tgatcaattt atagctaaat taccgccttt
     9901 cagccaattt gatcgagaac aatttatctc tttttgatgc
//
LOCUS       DAZPEP010000146         9674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZPEP010000146 DAZPEP010000000
VERSION     DAZPEP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.147"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1710
                     /locus_tag="ACHUT9_003471"
     CDS             <1..1710
                     /locus_tag="ACHUT9_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HFR1349864.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="ACHUT9_003472"
     CDS             1711..3360
                     /locus_tag="ACHUT9_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFR1349865.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPMQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="ACHUT9_003473"
     CDS             3365..4141
                     /locus_tag="ACHUT9_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFR1349866.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUT9_003474"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="ACHUT9_003474"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFR1349867.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="ACHUT9_003475"
     CDS             complement(5430..6752)
                     /locus_tag="ACHUT9_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFR1349868.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUT9_003476"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="ACHUT9_003476"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFR1349869.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUT9_003477"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="ACHUT9_003477"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFR1349870.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIKQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUT9_003478"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="ACHUT9_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFR1349871.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
BASE COUNT         2354 a         2483 c         2605 g         2232 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgatgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgcttg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaa
//
LOCUS       DAZPEP010000147         9645 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZPEP010000147 DAZPEP010000000
VERSION     DAZPEP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.148"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHUT9_003479"
     CDS             complement(<1..667)
                     /gene="ydcF"
                     /locus_tag="ACHUT9_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFR1349872.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHUT9_003480"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="ACHUT9_003480"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFR1349873.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="ACHUT9_003481"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="ACHUT9_003481"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFR1349874.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="ACHUT9_003482"
     CDS             complement(5698..7014)
                     /locus_tag="ACHUT9_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFR1349875.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="ACHUT9_003483"
     CDS             complement(7004..8761)
                     /locus_tag="ACHUT9_003483"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFR1349876.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..>9645)
                     /locus_tag="ACHUT9_003484"
     CDS             complement(8777..>9645)
                     /locus_tag="ACHUT9_003484"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFR1349877.1"
                     /translation="ALLILATLINRFLLWRLPERKGGEVTLRIRTWWGIVICFSLVIS
                     GPRWMTLAFFALISFLALKEYCMLISVHFPRWLYWVIPLNYLLIGFNCFELFLLFIPL
                     AGFLILATWRVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPSMNIQGGALLV
                     LFLLALTASNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMIASMIIGPLLT
                     PLNTLQAILAGLLIGISGFCGDVVMSAIKRDVGVKDSGKLLPGHGGILDRIDSLIFTA
                     PVFFYFIRYCCY"
BASE COUNT         2448 a         2628 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacaat cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaccga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtag attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata accaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggtggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcga
//
LOCUS       DAZPEP010000148         9626 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZPEP010000148 DAZPEP010000000
VERSION     DAZPEP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.149"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..221
                     /locus_tag="ACHUT9_003485"
     CDS             <1..221
                     /locus_tag="ACHUT9_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFR1349878.1"
                     /translation="VRVVISEFVADGTILMGDFSNVILANWEGQEVDYDDTSYRSSNT
                     IVYRVWDYSDIALAHSEAFVNIVIGKAE"
     gene            235..558
                     /locus_tag="ACHUT9_003486"
     CDS             235..558
                     /locus_tag="ACHUT9_003486"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349879.1"
                     /translation="MRVFKDTQCDAFLNAFGQSIQTCTGSTFTGIVEVLPVSIEAAGG
                     FIESVETYLTIRKDDLITANVAIGTVLIIDGVNQTIYNIEDDLSGMVNCYFRTSAGAS
                     FAEDY"
     gene            560..952
                     /locus_tag="ACHUT9_003487"
     CDS             560..952
                     /locus_tag="ACHUT9_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349880.1"
                     /translation="MMLVQKVRNTMKQLINATKNLTVSRDVDVFEQIAFDYSLTSLSF
                     SNERRAGNFAIQYLISPKPESGNTAPSITYDQIISTFDKEKTSAFSDAGLIMLSCSYE
                     QSDIVTDPITGSVSLSFTINIQVTEKTR"
     gene            970..3621
                     /locus_tag="ACHUT9_003488"
     CDS             970..3621
                     /locus_tag="ACHUT9_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HFR1349881.1"
                     /translation="MSDIFSGRELSVQYNADIGNRTPQGAGNVVIKQINTFPTLTINS
                     EANNFETYDSDYRTVLLSDKSVAPFDIVVNYLPDDPTHQFLDNAAKSQQLFQIIIQYQ
                     MNKDENTITYAMVNGYITSYKLDGDKGSVVTKAYSFAPHDVIARAMTINALSPIYQGD
                     YGLGSNTTDVPQYSPSVPTGNSFIKVPSSQAGNPAGADLMGVGLVDGTTVSSLAMTKT
                     GTLSIYAKNATTAWTRIYTATQMDGRYVPLTRTINGHALTANLVLNSEDTGSVPDTRT
                     VNGKPLSEDVVLTSTDTGSVPDTRTVNGHVLSEDIVLSSEDTGSVPDTRTVNGHVLSE
                     NVVLSSEDTGSVPDTRMVNGHVLSEDVVLTSTDVGLGKVTDDAQLTIAGNLSDLGDVE
                     EARINLAVYSMDEVDAEVTALADSITSLDSASMKKASNLSDLSSLSTARTNLQVNRLT
                     QGATYTDLMSNDLTNRIRIQDNGAWGASTGTGWIALGIGQGGTGATDALSARLNFQVD
                     RFNQGSNLTTIKSADSSAALVVYDGGQWGSATYIAGAFTGWKALAIPQGGTGVKTQAE
                     LWPAIRPTGATSLAGDPVSDSDATTKRWVENLLSSRTSVSPQWDVIASSPSSTAIGSY
                     VTGGRIRSICNIGTSTEISGGFRVGKTMGQTGQADLTLFISENGAEDKLWGLKGPTGT
                     ILTDVQSGWMTNTAVSGPTDANTITFNGMYAGPGDSGINYSEKYSPLLHMQRYPGANR
                     SQLQIDGGGTLRVRGGNGTTWFGWLVMQAQGTSGREFKRDITPANPVEALDRIMSQDL
                     VTFIYKDDEQERVRFGVIAEDAELTAPQYIKHDEETVEIEGEIVTRDHPRVDLNPIVM
                     DLMGAIQAQQEEINQLKDLVQQLLNNK"
     gene            3680..4153
                     /locus_tag="ACHUT9_003489"
     CDS             3680..4153
                     /locus_tag="ACHUT9_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1349882.1"
                     /translation="MAMDIFSGANLTVEVGSSAGKTVATDFVEVPEVNTFSTSGFEST
                     VISVKTFNNAYDRKLLGTKSIPDISLAVNYLPDNAVHQNLEQLADDQKRCQVKLSYFT
                     DATKKEGFFVIYTCFVSSTTIGGDKDEVVTKTFTLAVDGEAIDSGLITPKPESGE"
     gene            4205..4504
                     /locus_tag="ACHUT9_003490"
     CDS             4205..4504
                     /locus_tag="ACHUT9_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349883.1"
                     /translation="MNLNELKKKLQPELKPHEVMGEIIFIHRPNGQDFQKCTDVAKTL
                     ILCVKDENGDPIFAEEDIDGRINVNSIDFVIQNEIYSAIIALINTAKPVDEVEKK"
     gene            4537..4899
                     /locus_tag="ACHUT9_003491"
     CDS             4537..4899
                     /locus_tag="ACHUT9_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005138642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349884.1"
                     /translation="MVNKRGLSPEEYFSLDPDVLNMLMIYDTFIEPSGTQIEMMKHAY
                     QCYYTTISNGNLTPEARKSIKVQDFDFLDVLSDSTKSTSEKAEERKLKTKEAQSNDIK
                     SIGELIKSQVLGKKKNGK"
     gene            4889..7102
                     /locus_tag="ACHUT9_003492"
     CDS             4889..7102
                     /locus_tag="ACHUT9_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349885.1"
                     /translation="MANNNRIRVDITGDSSGLQRALRSGTESLEEFGSTAGGIVEEFT
                     GRITGMAGGFGTAMTGIAGAAAIGIGGLAALVESSREYVREMNQISKSTGLSVVQLQQ
                     LSAAFSGLGLEIDKFGDFNKDTLDKLGDAFRAGGGVSDDLKEYGLNLQDYNKYLKQAD
                     GGMQAVIHTFYAMRDAGKSQSEIVNVMETLASDSSHMISTLQQFNNEAQATAYIQSQN
                     AEVTNDAAEKYAEFDKNLGKLTTSIKGTIADGLSPLVNAMNSVYDAANQKPHEAGLFE
                     DLNERIKTSKGSLQDMLDIWEKLRTAGALNYQGAALNTGIMDDKGNKGNEFAQAKARL
                     EELANNLKSDVAVATAPTGGWVDQAKEAEDAAKKLEQQRKQAEAAQKAADAKRLQAQR
                     NLEAALAQVGEDGFQVRLQQFDRQQKALLKTITDSAQVLGINPDEMLKNATAYGAKQR
                     TDLLNSMVGYQDPNQGLKDTNALIGSGLLNDNQKGYLAQQQNQRINGGNPLAYNDTDQ
                     KLKDNADAMNAELQQNELLLKGHEDYEKRKAEITAKYNAQAIQISNQNAQDQLSIFSS
                     TAQSLSQGMVDAFGESSGAAQAAFTLSKSISIAQTVLSIQSALAQALATPWPASLANY
                     AQVLSLGMSIISTAKGAAAGQFHGGVDELPASYDNKSFVLKQGERVVQPEANKKLTAF
                     LDKQEGGSTSGDITVNAPLIIQGDVADDDKKFNEMLKKHANSVSQAVRSSQKRNT"
     gene            complement(7113..7685)
                     /locus_tag="ACHUT9_003493"
     CDS             complement(7113..7685)
                     /locus_tag="ACHUT9_003493"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349886.1"
                     /translation="MLPRISSFFIYLKTSIRIFFNRLDWQAASVIVAMLLFAGQIGYN
                     AYDKWKESSDDVFKMRVILLSEINNNLLALATGGDESQVLGTYEICIPLPTEDTEAIK
                     RVATAVSYLHDDVYYSQLEKLASLPKKEVDAIVKSYYSLSRLRTLSTQLAYNIKYSPP
                     KVKLLREEYYNLYTLSSSLSQYLSYHTENP"
     gene            7687..8244
                     /locus_tag="ACHUT9_003494"
     CDS             7687..8244
                     /locus_tag="ACHUT9_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349887.1"
                     /translation="MASFSNQIKVTNFQIKSTEPIYSNQTWTGQRIMRSTGIQYYQIQ
                     FTLNFNPASLNEVNSFLAQYAQGKPFTMSLGLAGTYYGKQTGAVTATALTKPGNMVVP
                     ISTNTLAVGEWVQFSNHNKLYRIVERTSTSITIFPALQNTVQASEALKYNNLMIEAVL
                     DPDNDYSLPIGNIMNITLKATENII"
     gene            8337..8867
                     /locus_tag="ACHUT9_003495"
     CDS             8337..8867
                     /locus_tag="ACHUT9_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2163 domain-containing protein"
                     /protein_id="HFR1349888.1"
                     /translation="MSLGVNVTCFDVLPKGTNGFHWTDSLIDIDLDGYKYISFPDIIS
                     GSLPSYSEQKGMSNDAINFKISNVNASVRALALGGFLKDAQMNIKLVILNPYDSTVID
                     SMLMFTGFIDYVQAVTDPNQKQNEMTIYVNSVYKKLDRQPALIAANSVYQSYYRNDEY
                     FSLLGQVNQNQNWKYK"
     gene            8867..9271
                     /locus_tag="ACHUT9_003496"
     CDS             8867..9271
                     /locus_tag="ACHUT9_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1349889.1"
                     /translation="MKNLHNEVMSIIQYSIDNPYKYGDNDCNIIVLRLIDLINGTTQL
                     TNRQYSSIKEGIAGLNKEGWNHTGEIVEAYCKPVQATIDGDIWLDPDNPLIMAVVASG
                     RVLGVNADHDGFELQPKPTEGTYYRVRKQRDG"
     gene            9264..>9626
                     /locus_tag="ACHUT9_003497"
     CDS             9264..>9626
                     /locus_tag="ACHUT9_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HFR1349890.1"
                     /translation="MGKSLGGFFGAIITAVVVAAAVYFSGGTALAAIGWGAAAGSLSL
                     VASSMLGQIGVSGYGDVADSLSRSTSPTTGLPVIYGGELPHKNGVSGGSFILTGSIVA
                     WYNVPNSDSQYLFSEQAVA"
BASE COUNT         3081 a         1751 c         2117 g         2677 t
ORIGIN      
        1 aagtacgtgt agtgatttct gaattcgtag cagatggtac tattctaatg ggcgatttct
       61 ccaatgtaat tctcgctaat tgggaagggc aggaagtaga ttatgacgat acatcctatc
      121 gttcaagtaa cactatcgta tatcgcgtat gggattactc agatatcgca cttgcacact
      181 ctgaagcttt cgtaaatatc gttattggta aagcagaata ataaggatta gataatgaga
      241 gtatttaaag atacgcagtg tgatgcgttt ctaaatgctt tcggtcagtc tatccaaact
      301 tgtacgggaa gtacttttac aggaatagta gaagtacttc ccgtttctat tgaggcggca
      361 ggtggattta tcgaaagcgt ggaaacatac ctaacaatca gaaaggacga tctaataacc
      421 gcaaacgtgg caattggtac agtactaatc attgatggtg taaaccagac aatctataac
      481 attgaagatg atctatcagg tatggttaat tgctatttcc gtacttctgc tggtgcttca
      541 tttgcagagg actactaata tgatgttagt acaaaaagta cggaatacga tgaagcagct
      601 tatcaatgct actaagaatc tgacagtatc acgtgatgtt gatgtatttg aacaaattgc
      661 ttttgattac tcattaacga gtttaagttt cagcaatgaa cgccgggcag gtaactttgc
      721 aattcagtat ctgatttccc ctaaaccaga atcaggtaat acagccccat ccatcactta
      781 tgatcagatt attagtactt ttgataaaga gaaaacttca gcattcagtg atgcaggttt
      841 aataatgctt tcgtgttctt atgaacaatc cgatattgtc acagatccta taactggctc
      901 ggtttcccta tcctttacga taaatattca agttacggaa aagacaagat aactaaaagg
      961 atataaagga tgagtgatat tttctcagga agagaattat cagtacagta taatgctgat
     1021 attggtaaca gaacaccaca gggagcaggc aatgtagtaa ttaaacagat taatactttt
     1081 ccaaccttaa caataaattc agaagcaaat aattttgaaa cgtatgatag tgattacagg
     1141 acagtactac tatctgataa atcagtagca ccatttgata ttgtagttaa ctacctacct
     1201 gatgatccta cacatcaatt cttggataat gccgctaagt ctcagcagct tttccagatc
     1261 atcattcagt accagatgaa taaggatgaa aataccatta cttatgcgat ggtgaatggt
     1321 tatatcacct cttataagtt ggacggtgat aaaggttcag tagtaaccaa ggcatacagt
     1381 tttgccccac atgatgtgat cgcacgtgca atgaccatta acgcactatc gccaatctat
     1441 cagggggact acggtttagg ttcgaacact acggatgtac ctcagtactc accatcagta
     1501 cctacgggta acagcttcat caaagtacca tcatctcaag caggcaatcc tgccggggcg
     1561 gatctgatgg gagtagggct tgtagatggc actactgtaa gttctcttgc gatgacaaag
     1621 acgggtactc taagtatcta tgcaaagaac gctacaaccg cctggacacg catatatacc
     1681 gctacacaga tggacgggcg atacgtaccc cttacacgta ccatcaacgg ccatgcactc
     1741 acggccaatt tagtactgaa cagtgaagat acaggttcag tacctgatac acgtactgtg
     1801 aatggtaagc ctctaagtga ggatgtagta cttaccagta ctgataccgg ctctgtacca
     1861 gatacacgga cagttaatgg tcatgtactc agtgaagata tagtactgag tagtgaagat
     1921 acaggttcag tacctgacac gcgtacagtg aacggtcatg tacttagtga aaatgtagta
     1981 ctgagtagtg aagatacagg ttcagtacct gatacacgta tggtcaacgg tcatgtactc
     2041 agtgaggatg tagtacttac cagtactgat gttggcttag ggaaggttac agatgatgcc
     2101 cagcttacga tagcaggtaa tctaagtgat ctgggggacg tggaagaagc acgtattaac
     2161 cttgctgttt actcaatgga tgaagtagat gcagaggtaa cggctttagc tgacagtatt
     2221 acttcccttg attccgcttc catgaagaaa gcgagtaacc tttctgatct atccagtctc
     2281 tctacagcac gtactaacct acaggtgaac cgtttgactc agggtgctac gtacaccgat
     2341 ctgatgagta atgacctgac taaccgtatc cgaattcagg ataacggggc atggggtgca
     2401 agtactggta ctggatggat tgctttaggt ataggtcagg gggggacagg ggctacagat
     2461 gctttgagtg ctcgcctaaa ctttcaggtg gatcgcttta atcaaggtag taatttaaca
     2521 actattaaat ccgctgattc ttccgctgca ttagtggttt atgatggagg gcaatggggt
     2581 tcagctacat atattgcggg tgctttcact ggctggaaag cattagcaat tccgcagggc
     2641 ggtactggtg ttaagactca ggctgagtta tggcctgcga ttcgtcctac gggtgcaact
     2701 tctttagcag gagatcctgt ctctgattct gacgcgacta ccaaacgctg ggttgaaaac
     2761 ttgctgagta gtcgtacttc agttagtcct caatgggatg ttattgcttc ttccccttcc
     2821 tctacggcaa ttggtagtta tgtaactgga gggcgtattc gttcgatatg caacattggt
     2881 acatcaactg aaatatctgg tggttttcgg gttggtaaaa cgatgggaca gactggacag
     2941 gctgatctca ccctttttat ttctgaaaat ggtgcagagg ataaactatg ggggctaaaa
     3001 gggccgactg gaacgatcct aactgatgtt caatcaggat ggatgactaa taccgctgtt
     3061 tcgggaccga cagatgccaa taccatcacc tttaacggta tgtatgcagg gccaggtgac
     3121 tcaggtatta actatagtga aaaatattcc cctttattgc acatgcaaag atacccaggg
     3181 gcaaatcgct ctcagcttca gatagacggt ggtggtactt taagagtacg aggcgggaac
     3241 ggtacaacat ggtttggatg gttagtaatg caagctcaag gtacatcagg tcgtgagttt
     3301 aaaagagaca taactccagc aaatccagtt gaagcattag atcggataat gtcacaagat
     3361 ttagtaacgt ttatctacaa ggatgatgaa caagaaaggg ttaggtttgg tgttatcgcc
     3421 gaagatgctg agttgactgc tccgcagtac attaagcatg atgaagaaac agttgaaata
     3481 gaaggggaga ttgtcactcg tgatcatcca cgtgttgatt taaatccaat agtgatggac
     3541 ttgatgggag ctattcaagc ccaacaagaa gaaattaatc agctaaaaga tttagtacaa
     3601 cagctattaa ataataaata aatataaacg gggtgaacgg attcactcca ataaaatttt
     3661 cttataagga tataaaacta tggctatgga tattttttcc ggtgctaacc ttactgtaga
     3721 agtaggttca agtgctggta aaacagttgc tactgacttt gtagaagtac cagaggtaaa
     3781 caccttcagt acttcaggct ttgaaagtac tgtaattagc gtaaaaacat tcaataatgc
     3841 ttatgatcgt aagctattgg gtactaagtc aatcccggac atttctcttg cagttaacta
     3901 cttaccagat aatgcagtac atcagaattt agaacaactt gcagacgatc agaaacgttg
     3961 tcaggtaaaa ctaagctatt ttaccgatgc aactaaaaaa gaaggtttct ttgtaattta
     4021 tacctgcttt gtttcaagta ccactatcgg tggtgataaa gatgaagtag taactaagac
     4081 tttcaccctt gcagttgatg gtgaagcaat tgattcaggt ctgattactc caaaaccaga
     4141 atcgggcgaa taataataaa taaagggaag gtgctcaact ttcccttttt tattggagat
     4201 gaaaatgaat ttaaacgaat tgaagaaaaa actacaacca gaattaaaac cacatgaagt
     4261 aatgggtgaa atcatcttta ttcatcgtcc aaatggtcag gatttccaga aatgcactga
     4321 tgtggctaaa actctaatcc tatgtgtaaa agatgaaaat ggtgatccta tcttcgcaga
     4381 agaagatatt gatggacgaa ttaatgttaa ctctattgat tttgtaatcc agaatgaaat
     4441 ttactcagca attattgctt taatcaatac tgcgaaacct gtagatgaag tagaaaaaaa
     4501 gtaagaggtg atatcgaatt aaagtacttt tgtaagatgg ttaacaaaag aggcttatca
     4561 cctgaagaat actttagttt agatcctgat gtactcaata tgcttatgat ctacgatact
     4621 tttatagaac catccggtac gcagattgaa atgatgaaac atgcgtacca gtgttactac
     4681 accactatca gtaatggcaa cctaacacca gaagcaagga aaagtatcaa ggtacaggat
     4741 tttgattttc ttgatgtgct tagtgatagt actaagagta cttcagagaa agctgaagaa
     4801 agaaaattaa aaactaaaga ggctcaatcc aatgatatta aatctatagg ggagttaatt
     4861 aaatctcagg tattaggaaa gaaaaagaat ggcaaataat aatagaatac gtgtagatat
     4921 tacgggtgat tcttccggtt tacagcgtgc tttaaggtct ggtactgaat cattagagga
     4981 atttggtagt actgctggtg gcattgttga agagttcaca ggccgaataa ctggtatggc
     5041 tggcgggttc ggtactgcta tgaccgggat cgcgggtgct gcggctattg gtattggtgg
     5101 cctggcagca ttagtagaat catcacgtga atatgtgcgt gaaatgaacc agatcagtaa
     5161 gagtaccgga ttgtccgtgg ttcagttaca acagctatca gcggcgttta gtggcctcgg
     5221 tcttgagatt gataagtttg gtgatttcaa caaggatacc ttggataagc tcggtgatgc
     5281 ttttcgtgct ggcggtggtg tatccgatga tcttaaagaa tatggactca acctacagga
     5341 ctataacaag tacttaaaac aggctgatgg tggtatgcag gctgtgatcc acacctttta
     5401 cgccatgcgt gatgcaggta aatcacagag tgaaatagtt aacgtaatgg aaacgttagc
     5461 cagtgactca agccacatga ttagtactct tcagcagttc aataatgagg ctcaagctac
     5521 agcctatatc cagtcacaaa atgctgaagt aactaatgat gctgctgaaa aatatgctga
     5581 attcgataaa aaccttggga aacttactac cagcattaaa ggtactattg ctgatggcct
     5641 atcaccttta gttaatgcca tgaacagtgt ctatgatgct gcaaatcaaa aacctcacga
     5701 ggcaggtttg tttgaagatc tgaatgaacg tattaaaact tccaagggtt ctctacagga
     5761 tatgttggat atctgggaaa aattacgtac agcaggggca ttaaactacc agggtgcagc
     5821 actcaatact ggcatcatgg atgataaggg caacaagggt aatgagttcg ctcaggctaa
     5881 ggcacgttta gaagaactgg caaacaacct aaagtccgat gttgcggttg ctaccgctcc
     5941 tactggcgga tgggttgatc aggcaaaaga agctgaagat gcagcgaaga agttagaaca
     6001 acaacgtaaa caggcagaag cggcacaaaa ggcggcagat gcaaaacgcc tacaggctca
     6061 acgtaatctg gaagctgctc ttgctcaagt tggggaagat ggttttcagg tacgtctaca
     6121 gcagttcgac agacaacaaa aggcacttct taaaaccatc accgatagtg ctcaagtact
     6181 cggtatcaat cctgatgaaa tgctaaagaa cgctactgca tatggtgcta aacaacgtac
     6241 tgatttactg aattcaatgg ttggttatca agatcctaat cagggtctga aagataccaa
     6301 tgctcttatt ggttccggtc tactcaatga caatcagaag gggtatctgg cacagcaaca
     6361 gaatcagcgt atcaatggtg gtaatccatt agcctataac gataccgatc agaagttgaa
     6421 agataacgct gatgctatga atgctgaact tcagcagaat gagttgttgc tgaaaggcca
     6481 tgaggactat gagaaacgta aagctgagat cacggcaaag tacaatgcac aggccatcca
     6541 aatcagtaac cagaatgcac aggatcagtt aagtatcttc tcaagtactg cacagtcact
     6601 atcacagggc atggttgatg cgtttgggga aagtagtggg gctgcacaag ctgcatttac
     6661 cctaagtaag agcataagca ttgcacagac agtactttca attcagtcag cattggccca
     6721 agcactcgct accccttggc cagcttcatt ggcaaactat gcacaagtac tttcacttgg
     6781 tatgtcgatt attagtactg caaagggtgc tgctgctggt cagttccatg gcggggtaga
     6841 tgaactacca gcaagttatg acaacaaatc atttgtactt aagcagggcg agagagtggt
     6901 gcagcctgaa gcgaataaga aattaacagc attcttggat aaacaagaag gtggaagtac
     6961 ttcaggtgat attaccgtta acgcaccatt gatcatccag ggtgatgtag ctgatgatga
     7021 caagaaattt aatgagatgc ttaagaaaca cgccaatagt gtttctcaag ctgtgagaag
     7081 tagtcaaaaa cgtaatacat aagaaagccc agttagggat tttccgtgtg ataagacaaa
     7141 tattgtgaaa gagaagatga aagggtatag agattgtaat actcttcccg caataattta
     7201 actttaggtg gtgagtattt aatattgtat gcaagctgtg ttgaaagagt acgtaatctc
     7261 gataatgaat aataactctt tacgatggca tctacttctt tttttgggag tgatgctaat
     7321 ttttctaatt gggaataata tacatcatca tgcaaataag aaacggcagt tgctactctt
     7381 ttaatagctt ctgtatcttc agttggaaga ggaatacata tttcatatgt gcctaacacc
     7441 tgagactcat cgccaccagt agcaagagct aaaaggttat tgttgatttc gctcaataag
     7501 attacacgca tcttaaaaac atcatctgaa ctttctttcc atttatcata tgcgttatag
     7561 cctatttggc ctgcaaataa caacatagca acgatgacgg atgcagcctg ccaatcaagc
     7621 ctgttaaaaa aaatacgaat cgaggttttt aaataaataa agaaagaaga tattcgaggt
     7681 aacaaaatgg catccttttc aaatcaaata aaagtaacga actttcaaat taaaagtact
     7741 gaaccgatct attctaatca aacatggaca ggtcaacgta tcatgagaag tactggaatt
     7801 caatactatc aaattcagtt cacccttaat tttaatcctg catcactaaa tgaagtgaat
     7861 agctttttag ctcaatatgc acagggcaaa cctttcacta tgtcattggg gcttgcaggt
     7921 acatattacg gtaaacagac tggtgcagta accgctactg ctctcactaa accgggtaat
     7981 atggttgtac ctatcagtac taacacatta gctgtgggtg agtgggtaca gttttccaat
     8041 cacaataagc tatatcgaat cgtagaacgt accagtactt caattactat tttccctgct
     8101 ctacaaaata cagtacaggc aagtgaagca ctcaaatata acaatctcat gatcgaggct
     8161 gtattagatc cagacaatga ttattcattg cctattggga acatcatgaa tattacattg
     8221 aaagcaacgg agaatatcat ctaatggact caggtatata tactaatgct aatcttctta
     8281 agtactggaa gttagtcaga ggtacaacca agacaagatt aacactaatg gaaattatga
     8341 gcttaggcgt taatgttact tgttttgatg tactaccaaa aggtactaat ggttttcact
     8401 ggacggattc actaattgat atagatctgg atggttataa gtacataagt ttccccgaca
     8461 ttatcagtgg ttcattacct tcatattctg aacaaaaggg catgtctaat gatgctatca
     8521 attttaaaat tagcaatgta aacgcctcgg ttcgtgctct tgcccttggt ggtttcttga
     8581 aagatgcaca gatgaatatc aagttagtaa ttctcaatcc atatgatagt actgtaattg
     8641 attcaatgct catgtttacg gggtttattg actatgtaca ggcagtaact gatccaaatc
     8701 agaagcaaaa cgaaatgact atatatgtga attctgtcta taagaaatta gacagacaac
     8761 ctgcattaat tgccgccaac tcagtatatc aatcttacta tagaaatgat gagtacttta
     8821 gtctgttagg tcaagttaat caaaatcaga actggaaata caagtaatga aaaatctaca
     8881 taatgaagta atgagtatta ttcagtactc aattgataac ccatataagt atggtgataa
     8941 cgattgcaac attatagtac tacgccttat tgatttaatt aatggtacta cacagttaac
     9001 taatcgccag tattcgagca ttaaagaagg tatagcaggt ttaaacaagg aaggatggaa
     9061 ccatacaggg gaaattgttg aagcgtactg taagccagta caagccacta tagacggtga
     9121 tatctggtta gatcctgaca acccattaat tatggcagta gtggcatctg gtcgggtact
     9181 tggagtaaat gctgatcatg atggtttcga acttcaacca aaaccaacag agggaacata
     9241 ttacagagta aggaaacaga gagatgggta agagtttagg gggctttttt ggagccataa
     9301 ttacggcagt tgtcgtcgcg gctgctgtgt acttttcagg aggtacagca ttagcagcaa
     9361 taggttgggg tgcagcagcc ggatctttgt cattagttgc aagttctatg ttaggtcaaa
     9421 tcggggtttc tggatacggt gatgtagcag actcactaag tagaagtact tcaccaacta
     9481 ctggattacc cgttatatac ggtggagaac taccacataa aaatggtgta tcaggcggtt
     9541 ccttcatcct aaccggaagc atagtagcct ggtacaacgt accaaattct gattctcagt
     9601 acttattctc agaacaggct gtagca
//
LOCUS       DAZPEP010000149         9621 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZPEP010000149 DAZPEP010000000
VERSION     DAZPEP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9621
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.150"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..2199
                     /locus_tag="ACHUT9_003498"
                     /pseudo
     CDS             1..2199
                     /locus_tag="ACHUT9_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            2192..3328
                     /locus_tag="ACHUT9_003499"
     CDS             2192..3328
                     /locus_tag="ACHUT9_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFR1349891.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="ACHUT9_003500"
     CDS             3325..5325
                     /locus_tag="ACHUT9_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFR1349892.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5450..5911
                     /locus_tag="ACHUT9_003501"
     CDS             5450..5911
                     /locus_tag="ACHUT9_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFR1349893.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5952..6422)
                     /locus_tag="ACHUT9_003502"
     CDS             complement(5952..6422)
                     /locus_tag="ACHUT9_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFR1349894.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHUT9_003503"
                     /gene_synonym="yehT"
     CDS             complement(6469..7188)
                     /gene="btsR"
                     /locus_tag="ACHUT9_003503"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFR1349895.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHUT9_003504"
     CDS             complement(7185..8870)
                     /gene="btsS"
                     /locus_tag="ACHUT9_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFR1349896.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9092..9157
                     /locus_tag="ACHUT9_003505"
                     /pseudo
     CDS             9092..9157
                     /locus_tag="ACHUT9_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9385..>9621
                     /locus_tag="ACHUT9_003506"
     CDS             9385..>9621
                     /locus_tag="ACHUT9_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1349897.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEW"
BASE COUNT         2354 a         2531 c         2468 g         2268 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgctttttac
     5341 taagagttct actccagttc cggactgctc acgccacggt attaggcata tcctatatag
     5401 cccctggtga gagtcaccag ttccttgatt aaataaaatg gagttttaca tgaaggcttt
     5461 caataagctg ttttccctcg ttgttgcatc tgttctggtt ttctctcttg ctggctgcgg
     5521 tgacaaagaa gaatcgaaga aattcagcgc caatctgaac ggcactgaaa ttgccattac
     5581 ctatgtctac aaaggtgaca aggtgcttaa gcaatcttct gaaaccaaaa ttcaatttgc
     5641 ctccattggt gcaaccacca aagaagatgc tgccaagaca cttgagccgt taagcgccaa
     5701 atacaaaaac atcgcgggtg ttgaagaaaa attaacctat accgatacct acgcgcagga
     5761 aaacgtgact atcgatatgg aaaaagtgga ttttaaagcc ctgcagggta tttcaggaat
     5821 caacgtttct gctgaagatg ccaaaaaagg tatcactatg gcgcaaatgg aactggtgat
     5881 gaaagccgct ggttttaaag aagtgaaata atctgtcggc ggccatgttt cgcatggccg
     5941 ccatacccgt cttagctttt cttcacatgc tggcgcgctg ccagtccacg cagaaaataa
     6001 cgtaaaaatt gatcgccgca ttcgcggaag tttttatgat ccggtgcgcg catcatcgct
     6061 gtaatttccg gcatcgaaac gcggaactgc tgttcggtga ggatcgccag aatgtcatcg
     6121 gttttcagcg aaaacgcgat gcgtaatttt ttcagcacga tgttgttatt aatgcgacgt
     6181 tccggctcca gtgccggagc agactcatcc ttgccgcgtt tttcataaat caggccattg
     6241 aggaatgacg acaaaacaat gtccggacaa cgctgaaaac cctcttcgtc ttctttacgt
     6301 agccagacgg cgatctgttc cgcggtggct tcgacattac ccagcgccag aatacgcacc
     6361 aggtcattat tattggcttt caaaatgtag cgcacgctgc gcagaatatc gttacttagc
     6421 atgaggcctt caggtgttga tgaggcaaaa agccatttta gcagtctttt acaggccaat
     6481 cgcctctttt aagcttttca gataacggcg gctgaccggc acggttaagc cattacgcaa
     6541 aatcaactcg gcctggccgt tatcttccag acgaatctcc tgtaaatgcg cgaggttaac
     6601 cagatactga cgatggcagc gcagtagtgg tgtacgactt tccagggtac gtaatgtcaa
     6661 ttcggtaaag ccctctttcc cttcgtggct ggtaacgtag acaccgctca tccgactgct
     6721 gacaaatgcc acatctttca tttgcagcaa ataaatccga ctatgccccg tacaagggat
     6781 aaatttcagc gcctgttgat tttccggtaa cagcgaaaca tcctgcttgc tgcgctcctg
     6841 acgcaatcgc gccagcgttt tctccagtcg cgcttcatca attggcttca gcagataatc
     6901 aaaggcatgt tcttcaaagg ctttaatagc gtattcgtca aacgcagtga gaaaaacaat
     6961 atacgggcga tgttccgggt caagcatccc caccatttcc agaccactga tgcgcggcat
     7021 ctggatatcg agaaacagca catccgggcg cagtttatgc accgcgccga tcccttccac
     7081 ggcgtttgaa cactctccaa cgatttcaat atcgctctgc tcctgcaaaa atacacgcag
     7141 gttctcccgt gctaacggtt catcatcgac aattaagact ttaatcatgc ctcgtccctc
     7201 catggtagtc gtaacgttat tcgggtgtaa ctatcaggct cacaggcgac gcttattcca
     7261 tagtcatcgc caaaccgttc acgtaaacgc ttatccacca gattcatccc cagcccactg
     7321 gcattggtta ccggttgata caaaccggca ttgtcttcga tctccagcat caaatgttgc
     7381 ccctcacgtc gggcgctgat tgccactcgc cctgtatcca gcagttgtga tgtcccatgt
     7441 ttaatggcgt tttccactat cggttgcagg gtaaacgcgg gcaattgctg ctgggataat
     7501 tcttgcggaa tagcaatgtt gacctgcaac cgcgactgga agcgcgcctt ttcaatttgc
     7561 agataagcgt tcacatgttc aatttcgtcg gcgagagtaa caaactccga aggccgcttt
     7621 aagtttttgc ggaaaaaagt ggaaagatac tgcaccagct ggctggcctg ttcgctgtcg
     7681 cggcggatca ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg
     7741 gcgtgaagca gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgcccg
     7801 gcaagaatct gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa
     7861 ctgaataaac ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg
     7921 ccacgcaacg gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga
     7981 taaggtactt cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg
     8041 taaggcgaag aaatcggttt gccaggtaaa tggtggtcgt caccaattcc ggtaaaggcc
     8101 agcaatttct ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagcacc
     8161 tgagccactt tcatgctgtt cacttcgtta aacccctgtc gcaaaatgcc ttccgtcgag
     8221 gctgccactt tcagcgcagt ggcagaaaaa gccgaagtgt atttttcaaa catcgcgcgt
     8281 ttatcgagca atatacgcat aaacagcgcc gcgccgacgg tattggtgac catcattggc
     8341 gcagcaatat tactcaccag acgcaccgca tcttcataag gtcgggcgat cgccaggatg
     8401 atcagcattt gcaccatttc agcgacgaac gtgacggcac cggcggtaat ggggttaaag
     8461 actttatcag tgcgcccgcg acggatcagg atgctgtgta ccaggccgcc gagcaatcct
     8521 tcaacgatgg ttgagatcat gcaacttagc gcggtcatgc cccccatcga atatcgatgt
     8581 aagccgccgg tcagaccaac cagcccaccg acgaccggac cgccgagtaa gccgcccatt
     8641 accgcgccta tcgcacgggt attggcaata gaatcgtcaa tgtgcaaccc aaaccaggtg
     8701 cccatgatgc agaagatgga aaagacgatg tagcagagaa atttatgcgg cagacgaacc
     8761 gtgacctgca ttaacggtat gaataatggc gttttactca ttaaccacgc aatgactaaa
     8821 aaaacgcaca tctgctgaag cagcagcaac accagattaa aatcgtacat acccgcaaac
     8881 cacacttccc tttaaaacgc gtaacataca ttgcctgcgt ttaactttct ttgaactctt
     8941 gcagaaaaat gagaattcgt gagtacgatc actcaaaatc gcctggcaaa aataaaatca
     9001 ccctatagat gcacaaaaaa cgggcaaaac tacctggttc gcaaaactgc gtctaaagtt
     9061 aaaccgggac ctcgcgagca agggtgagac gatggcgctt tacacaattg gtgaagtggc
     9121 gttgctttgt gatattaacc ctgtcacgtt acgcgcgtga cgcaacattt ttaaaaatcg
     9181 acgaagatcg tccaggagcg ccgtttttca agggttggat agatgattcg tttaccatgt
     9241 cgacgaactt cgacgaattt cgccgataaa aagtgtccca tacatgcccc attgatgccc
     9301 cataaacaat atcaggaatt gacactggtt atccatacag ataaaaataa cacctgtaca
     9361 taaccacagt tacaggaggg tgttatgcgt gttgagatct gtatcgctaa agaaaaaatc
     9421 actaaaatgc caaacggtgc tgtggatgcg ttaaaggaag aattaacccg acgcatcagt
     9481 aaacgttatg acgatgtaga ggtgatcgta aaagccacca gcaacgatgg cctttctgtt
     9541 acacgcaccg cagataagga ttctgcaaaa acatttgttc aggagactct gaaagatacc
     9601 tgggagtctg ctgacgagtg g
//
LOCUS       DAZPEP010000150         9501 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZPEP010000150 DAZPEP010000000
VERSION     DAZPEP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9501
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.151"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..108
                     /locus_tag="ACHUT9_003507"
     CDS             <1..108
                     /locus_tag="ACHUT9_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1349898.1"
                     /translation="HRTIIAFAVDDNKVFIAGVYHGGQIYETDFLSSAP"
     gene            complement(149..1231)
                     /locus_tag="ACHUT9_003508"
     CDS             complement(149..1231)
                     /locus_tag="ACHUT9_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFR1349899.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3060)
                     /locus_tag="ACHUT9_003509"
     CDS             complement(1228..3060)
                     /locus_tag="ACHUT9_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFR1349900.1"
                     /translation="MARMFDTLGFYIMNVQHFDFFTDNEFSNLERYQTCKAMAKGYAG
                     NYKTGQSRLMHARSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAE
                     RTGELIAQFPAEDAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDF
                     SHASVIDPACGGGAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLV
                     LLESVLMPLCVKVKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKY
                     SRSLFGHANLYGLFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYA
                     LDFVTDRDGVFDDVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKG
                     GATWLLPRVKDDACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPL
                     VWAESITSAGFRFSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAI
                     LPQGFIDETGGVVVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVS
                     AYELNSIPLPSLEQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="ACHUT9_003510"
     CDS             complement(3275..3631)
                     /locus_tag="ACHUT9_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349901.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="ACHUT9_003511"
     CDS             complement(3689..4111)
                     /locus_tag="ACHUT9_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1349902.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="ACHUT9_003512"
     CDS             complement(4127..4852)
                     /locus_tag="ACHUT9_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFR1349903.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="ACHUT9_003513"
     CDS             complement(4874..5620)
                     /locus_tag="ACHUT9_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1349904.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="ACHUT9_003514"
     CDS             complement(5627..6691)
                     /locus_tag="ACHUT9_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFR1349905.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="ACHUT9_003515"
     CDS             complement(6763..7188)
                     /locus_tag="ACHUT9_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1349906.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="ACHUT9_003516"
     CDS             complement(7185..7448)
                     /locus_tag="ACHUT9_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1349907.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
     gene            7560..8060
                     /locus_tag="ACHUT9_003517"
     CDS             7560..8060
                     /locus_tag="ACHUT9_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1349908.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            8080..8274
                     /locus_tag="ACHUT9_003518"
     CDS             8080..8274
                     /locus_tag="ACHUT9_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFR1349909.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            8274..8564
                     /locus_tag="ACHUT9_003519"
     CDS             8274..8564
                     /locus_tag="ACHUT9_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFR1349910.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            8844..8996
                     /locus_tag="ACHUT9_003520"
     CDS             8844..8996
                     /locus_tag="ACHUT9_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1349911.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            9008..9373
                     /locus_tag="ACHUT9_003521"
     CDS             9008..9373
                     /locus_tag="ACHUT9_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001320327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1349912.1"
                     /translation="MEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARKIKTA
                     FVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRRGLDG
                     SYDHDVLQVLRTMVTSLGI"
BASE COUNT         2443 a         2491 c         2017 g         2550 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatt tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ctggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatagc actgttattt
     7501 ttaaaagtaa atagtgatgc tatttcaaag tgattagcaa tcttattaga attggatgta
     7561 tgaaaagaaa atcattgtca gacaccgacc tcaacgccgc cagaagactg aaagaaatct
     7621 ggatggcaaa gaaatctcag ttgggattga ctcaggaacg tgcggcggaa attatggggt
     7681 tttcaacaca aggggcggta agtcactacc taaatggtca aaccccacta aaccttgagg
     7741 ctgttctaaa gtttgctggc ttactgaaag ttccccctga gtcaatcagg ccagacatgg
     7801 tagatttgct gcaaattgca aggatgtatt gtcgagaacc acagcaagat aacatagtcg
     7861 cattaccagc agacacagaa caaacagagg atgaactacc gtttgccgta gagccgatgg
     7921 aacgagattt aatccatacg ttcagagctt ttccgaaaga agatcaagaa cacatgcttc
     7981 aggagatgaa agataaaaaa gaatcaatgg atcgtacggt tgcgcggtgg ttagctgcac
     8041 aaaaaggccg tcgcgcctaa atcagaggag atatgtaaaa tgagtacagt cctttcccca
     8101 atggtttcag aatttgagac agttgagcaa gaaaatagtt acaacgaatg gctgcgggcc
     8161 aaagtagcag caagcctcgc ggatccccgc ccggcgattc cgcatgatga ggtaatggcg
     8221 gaaatggaac aaattattgc ccaaattgct gctgcaactc acagaggtga ataatgttac
     8281 ccgttttatg gctaccatct gctcgtgatg atttgcgcca gatcgtagcc tacatagcta
     8341 aagaaaatcc cgcggcagca cgcagaatga aaattcgaat tgaaacatcc gttttgccgc
     8401 tcacagagca tccttacctg tatcctccaa gcgagcgagt tcttggtctt cgtgaaattg
     8461 taactcatcc taactacatc atcctttacc gagtaacagc atcaagcata gaagtagtaa
     8521 atattgtgca ttctcgaaga cagtatccag gcaaaaacag ttaattattc ccgccgacag
     8581 aaccacctta gggtggtttt ttcttgcccc aacaaaaaac aatagcgatg ctatttacat
     8641 caaaaaaaat aatgatgcta ttgtttcagg cgtcaaccca ccccgcccca cagaatgcag
     8701 ggcaatactt cgagttacca gacagtggtc aggggttaag tagccagccc gaggcgtaag
     8761 aacatgacgg cagggttcaa tctttaacta tgcagcaggt tttttgttcc gccaccccgg
     8821 cgttaagggg aaatgaggtc agcatggata ctatcgatct tggcaacaac gaatctctgg
     8881 tgtgcggcgt gttccctaac caggatggaa cgtttaccgc catgacgtat accaaaagca
     8941 aaacgtttaa aaccgaatct ggcgcgcgtt gctggttagc cagaaacact gactgatgag
     9001 gctgacgatg gaatttaaag atttaccgtc tgatgtacag aaaacagcag ctcatacatt
     9061 gcattctgtg ctgcgagaaa tcgggaaaga tattgcaagc gagccagcaa aagatctggc
     9121 ccggaaaatc aagaccgctt tcgttgagct ttataatgtt ggcactgact ctgaaactgt
     9181 cgagaccaag accgtaagtt caccaatatt ctcacttgag ccaagcgtat tatcaggtga
     9241 aatatgcacc gagatttcca gcgaactgct tccggtaata cgagaagcaa ttcgccgtcg
     9301 cggtttggat ggaagttatg atcatgacgt tctgcaagtt ctcaggacaa tggtgacttc
     9361 actggggatt tgatccctgc gtcctctaag cttttgatat agcggtcctc caagaagcgg
     9421 taaattttgt cgaaatcatc ttttccctct ggtttattca gaggattaca ggacccgtaa
     9481 gtagcggcat acatttcaac c
//
LOCUS       DAZPEP010000151         9496 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZPEP010000151 DAZPEP010000000
VERSION     DAZPEP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9496
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.152"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            122..952
                     /locus_tag="ACHUT9_003522"
     CDS             122..952
                     /locus_tag="ACHUT9_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HFR1349913.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUT9_003523"
     CDS             complement(1093..1866)
                     /gene="uxuR"
                     /locus_tag="ACHUT9_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFR1349914.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2081..3541)
                     /locus_tag="ACHUT9_003524"
     CDS             complement(2081..3541)
                     /locus_tag="ACHUT9_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFR1349915.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUT9_003525"
     CDS             complement(3622..4806)
                     /gene="uxuA"
                     /locus_tag="ACHUT9_003525"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFR1349916.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5146..6489
                     /gene="gntP"
                     /locus_tag="ACHUT9_003526"
     CDS             5146..6489
                     /gene="gntP"
                     /locus_tag="ACHUT9_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFR1349917.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDTATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGQLELVNSVVGLIIVLIISMVA"
     gene            complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHUT9_003527"
     CDS             complement(6653..7555)
                     /gene="fimH"
                     /locus_tag="ACHUT9_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1349918.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHUT9_003528"
     CDS             complement(7575..8078)
                     /gene="fimG"
                     /locus_tag="ACHUT9_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFR1349919.1"
                     /translation="MKWRKRGYLLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNSGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHUT9_003529"
     CDS             complement(8091..8621)
                     /gene="fimF"
                     /locus_tag="ACHUT9_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFR1349920.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHUT9_003530"
     CDS             complement(8631..>9496)
                     /gene="fimD"
                     /locus_tag="ACHUT9_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1349921.1"
                     /translation="KSYCLNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLA
                     GVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQL
                     YYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYAT
                     EYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPL
                     PFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQ
                     QLLTQLSAECR"
BASE COUNT         2260 a         2483 c         2376 g         2377 t
ORIGIN      
        1 gtgtagagtt gatatcacta caacagaaac atgcatgtca tttgtttact tctcttttta
       61 ctacagaaaa atttatctat ttttctaaac atcagtgaaa tactcataaa aggtaactat
      121 tatgtctatg ccattaagca atgcccttca gagtcaaatt attacagata atcacttcct
      181 tcatcaccca atagtcgaca gtgagtttac acataaatta aaatatgcac gcatggattt
      241 ggaaaacatc tatcttccgc ctcttactcg tggcaataac cataattacg atggaaaaag
      301 cgctgtcgaa attagaaaac ttgatattag taaagagtcc tggccattta attatgtgac
      361 aggagcatgc agagaatccg atggtattac aacaacagga cgtatgctct atcggaactt
      421 gaaaatcacc tcagctttag acgaaatata tggtggcata tgtaaaaagg cgcatgctgc
      481 cacagagtta gcagaaggtt tacggctaaa tttatttatg aagagccctt tcgatcctgt
      541 tgaggactat acagttcatg aaataacatt aggccctggt tgcaatgttc ctggttatgc
      601 cggaaccacg ataggatata tttcaaccct tccagcttct caggcaaaaa gatggaccaa
      661 tgagcagcca aggattgata tctatattga tcagataatg acggtaacag gggtagcaaa
      721 ttcttccgga ttcgcccttg ccgcattact taatgctaat attgagttgg gtaatgaccc
      781 aataattggt atagaagcct atcccggtac cgcggagatc catgcaaaaa tgggatataa
      841 agttatcccg ggtgatgaaa atgcaccact caaacgtatg actctgcaac cgtcatctct
      901 tccagaactg tttgaattaa aaaatggtga gtggaattat attggtaaat aactaccagg
      961 aagaatgagt actaacattc aggttataca gatagctaaa caataccctg caatttacaa
     1021 agtaatactc attgcgactt cttaatttaa gaaagcgcaa ggaacattta cccttgcgct
     1081 tattataata agtcaggcgt caactttatc cagcggccat gaatcaaaca gatagccatc
     1141 aaaataaata tcgtcaacgt tcgagaattc cagcagacgt tgcttaacgt tttccagatg
     1201 ccgccacatt gccagcttcg ccgctcgggc atcttttttg attaacgcgg cgaggatctg
     1261 tttgtgatcg cccaaccact ctttgcgata caggctgtca tccagatggc tgtgcaactg
     1321 gatccacatt ggattgtttt cccgccactg ccaggactga cggaacagct ccaccagcat
     1381 gctgttatgc gttgcttcgg caatggcgag atggaactgc atatcaccac tttcgctgct
     1441 gcccggcgcg ctggaagcca gctcacgctc ttccagttgc aatgcctgac gcattttgac
     1501 gatatcttcg cgggtagcct gcaaagcggc aaattccgcg atgttacttt ccagtaactg
     1561 acgcgcctgt aacagctcaa aaggaccggc atcattgcag acgttagcat ccggactgtc
     1621 tgtgttgtgg ctgcctgagc tatcaagaac atagataccg gcaccccggc gcacttccac
     1681 cagccctttg atctccagca tgatcagcgc ttcacgtacc accgtcctcg tgacatcaag
     1741 catttctgca atttcacgct ccggcggcag ccgttcgcca ggattgtatg gcgtctttat
     1801 gatcagatcg cggatcatcg ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga
     1861 tttcatggtg ttgtgtccgt taggtgcggt taatcaaatt tttgccggat gcggcgtgaa
     1921 cgccttatcc ggcctacaac gggcaatttt acggcacccg ttaatctcaa actataccat
     1981 tagcactgga agttggcacg tacaggtggg caaaaaaacg aaaactggtc aaccaaatat
     2041 cagtttgatt gaccagtaag tctgtagggt aatcagttag ttacagcgca gccacacact
     2101 cgcgcgcacc gcgttcgcac agctgctgat atgccgccgt caccgcgcca acaaagtcgg
     2161 cattctgcgg cagatcatcg gcaaaaatgc cgctcaggcc cagcagcgct ttcacgcggt
     2221 ctgcgccctg atactgcgcg ttaatcttct ggaactcggc cagcatcggg tcaaccacgt
     2281 caatggcatt accctgttca tccacgccct gggtgtagcg catccagcca gccacgccca
     2341 gtgccaggtg acgccagctg ccgccgtttt gcaggtgcag acgcaccgga tccagaagac
     2401 gctgcggtaa cttctggctg ccgtccattg caatctgcca ggtacggtga cgcagagacg
     2461 ggttgctgaa acgctcgatc agcagcgtcg cataggcgtt caggtctgta ccttccggca
     2521 tcgacagcgt tggcgcttgt tcctgcatca tcagggcaaa ggccgctttg cgataatccg
     2581 gattagtcat ggtgtcggca atggtttcat agccgccgag gtaaccgagg tacgccagga
     2641 aagagtggct gccgttcagc atacgcagtt tcatcatttc gaacggcaca acgtctgcaa
     2701 cgaactgtgc gcccacttta tcccagtccg gacgaccatt aacgaagtta tcttcaatca
     2761 cccactgacg gaacggttcg caggcaatgg cgcacgggtc gtaaacaccc agctggtcag
     2821 caatttcctg taaggtttct ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg
     2881 ggaaggtgac gttctcttca atccatgccg ccagctgcgg atcacgtgcc tgagccagcc
     2941 ccagaaccgc gaccttcgcc acatgaccgt tttcacgcac gttgtcgcag gacatcaccg
     3001 taaacgcttt cagccctttt tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg
     3061 cggacttcgg cgcagtcggg ttttccagat cgtgcttgat cagcgggtta ttgagatcca
     3121 gctgaccgct tgccgcatcg gcgcaataac ctttctctgt gaccgtcagg gagacaatcg
     3181 ccgtctgcgg acgcgccatc gcgttgagaa taccttcaca accgtcgatt tccggatgca
     3241 gggcttcttt catcgaaccg ataattttca gctcggtgct ttctgcgcct ttttccgcca
     3301 cggtgtacag cagttgctgt tttttcaggt tttcaatcag cacacggtcg ttgcctggca
     3361 tcaggttaac ttcgcagata ccccagtcgc tgtcggtact ttccagcaga tgatgggtgt
     3421 acagcgcctg gtgcgcgcgg tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca
     3481 gacgagaatg atcccatgac gggcgggcaa ccggcagatt gctgtcaaca atagtagtca
     3541 tcgtaaactc cagaatccgt cttcagagag tgacggcaag gaagaaggaa ccggcacggc
     3601 gcgcagccat gccggtgggt atcaacggct aaagaaagcg cgctggatcg ccagttcgac
     3661 accgcgaact tcagccaggc ctttcagacg accaattgcg gagtaacctg ggttggtttt
     3721 cttcttcagg tcgtccagca tctgatgacc gtggtccgga cgcatcggga tcaggtcttc
     3781 tttgccttcc gctttacgac ggtgttcttc ttcaacaatc gctttcacca cttcgtacat
     3841 atcaacgtca ccgttcaggt gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat
     3901 ggtggagcgc agatgggtga agtaaatacg cggaccgaac tgcttgatca tatcaaccag
     3961 atcgttgtca gcacgcacgc cgtaggaacc tgtgcacatg gtgaaaccgt tcgccatgct
     4021 gtttacggta tcaaccatcc actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc
     4081 gaggatcggg cgcggcggat cgtccgggtg aacagccata cggacgccaa cttcttcggc
     4141 aaccggaata atcgctttca ggaagacggc aaagttttcg cgcagtttgg ctttgtcgat
     4201 atctttatac agctccaggt gcttacggaa ctggtcgagg gtataccctt cttccgcgcc
     4261 cggcagaccg gcaatgatgt tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc
     4321 gaagcgttca gcggcctgag caatttcttc ttcggtgtaa tccgcttccg cgcctggacg
     4381 cttcaggata tgcatttcga atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga
     4441 gccgtctggc agcacgtatt cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt
     4501 gtagcacacg gtgcgaatgc cgcactgcgc caggttgcgc agagtctgct gatagttagc
     4561 gatccactgt tcatagttgc cagtgtgggt tttgatatct tcgtgaattg gcacgctttc
     4621 tacgacagac cataccaggc ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc
     4681 tacggaccat acttcgccgt tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc
     4741 cgcctgacgg acatcagcta gagaaaccgg atcgtttggg ccgtaccagc gccaggtctg
     4801 ttccatgatt tttcctcttt cacatagtgg gttccacagc aaagagacgt ggagccccgg
     4861 agttgtttag gtatccggat gaacaaccag accgcagccg tggcgttgca tcggatgaga
     4921 tggcgtataa gttctacctt ttggtaagac atgtcaacca gaaatctgaa attggttaac
     4981 cacatcgcaa gaatttcact ctcacggttg ttgctgcaaa ttgcagcaaa gggtaatcga
     5041 tggttattga tcagaggatg tgacattcat cgcaacaatg gttgaccaat ttacataaca
     5101 tgtcgcccaa aataacaacg gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac
     5161 attctctggg tggtattcgg cattggcctg atgctggtac tgaatttgaa gttcaaaatc
     5221 aattcaatgg tggctttgct agtggcggcg ctgtccgtcg ggatgctggc gggcatggat
     5281 ttgatgtcgc tgctgcacac aatgaaagcg ggcttcggca acacgctggg ggaactggct
     5341 atcatcgtgg tgttcggtgc ggtcatcggt aaattgatgg tcgactccgg cgcggcgcac
     5401 cagatagcgc atacgctgct ggcgcgtctc ggtctgcgct atgtacagct gtcggtaatt
     5461 atcatcggcc tgattttcgg tctggcgatg ttctatgaag tggcctttat catgttagcg
     5521 ccgctggtta ttgtcattgc cgccgaagct aaaatcccgt ttctgaaact ggcgatcccg
     5581 gcagtagcag ctgccactac cgcacattca ctgttcccac cgcagccggg tccggtggcg
     5641 ctggtgaatg cttatggcgc ggatatgggg atggtttata tctatggcgt actggtgacg
     5701 atcccaagtg tcatctgcgc aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc
     5761 ccaacgccat cattcctgaa agcagatcaa ccggtagata tgaataatct gccctctttc
     5821 ggcgtttcga ttctggtgcc gctgatcccg gcgatcatta tgatctctac caccatcgcc
     5881 aatatctggc tggtaaaaga tacccctgcc tgggaagtgg ttaactttat cggttcctcg
     5941 ccgattgcaa tgtttattgc gatggtggtt gcattcgtac tcttcggcac cgcgcgcggg
     6001 cacgatatgc agtgggtgat gaacgctttt gaaagcgcgg tgaagagcat tgcaatggtg
     6061 attctgatca tcggtgcggg tggcgtgctg aagcagacca tcatcgacac cggcattggt
     6121 gacaccatcg gcatgttgat gtcccacggc aatatctcgc cctacatcat ggcatggctg
     6181 atcaccgtac tgattcgtct ggcgacgggt cagggtgtgg tttcagcgat gaccgccgcc
     6241 gggattatca gtgctgcaat ccttgataca gcaactggtc agctggttgg cgtgaatccg
     6301 gcgctgctgg tgctagcgac ggctgcgggt tccaacaccc tcacccacat taatgatgcc
     6361 tcattctggc tgttcaaagg ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg
     6421 ggtcagctgg agctggtcaa ctccgtggtt gggctgatta ttgtgttgat tatcagcatg
     6481 gtagcgtaaa agagcaccag agcctgccat tggcaggctc tggtcggatt gtcggcaaag
     6541 tgcgctttgt ttattcttat ctggcctaca aagggctaac gtgcgggttt gtagatagcg
     6601 tagcgcatca ggcaatattg cgtacctgca ttagcaatgt cctgtgattt ctttattgat
     6661 aaacaaaagt cacgccaata atcgattgca cattccctgc agtcacctgc cctccagtac
     6721 gtgcgtaatt tgccgttaat cccagactta ccgccgaagt ccctactgct cctaacgata
     6781 ccgtgttatt cgctggaata atcgtaccgt tgcgcgtcaa ctgtacgccg acgccctgcg
     6841 ctggtgaaaa cgaggcggta ttggtgaaaa tcgaattgcc cgcatctgcg gttgtgccgg
     6901 agaggtaata tcccaggttt tggcttttcg cacaataaac ggtaagagga attggcactg
     6961 aaccagggta gtccggcaga gtaacggtga catcacgagc agaaacatcg cagccgccag
     7021 tgggcaccac cacatcatta ttggcgtaaa tattccacac aaactgaaaa tcatcgctgt
     7081 tatagttgtt ggtctgtcgc aaaataagca cggcaattaa tgaaccagct ttaatcgcca
     7141 ccccgccagc actgctcaca ggcgtcaaat aaagcgccac cggccacggc ttatccgttc
     7201 tcgaattata aacaacccgc ggcgtttcgc tggtggtagg aaatggatag ctactgccac
     7261 tatattttac ggtcccggaa aaattagata acacgccgcc ataagccgag cctcgttgca
     7321 gtgtgacata gtctgtaatg gtttccggat aatcgttatg gcaaaagatt tgcgtcgaaa
     7381 gatccacgac caggttttgc cccacattca cggcaggcgc aaggtttaca taaacattag
     7441 cgctgccacc gccaataggg atagcggtgc cattggcggt tttacaggcg aatgaccagg
     7501 catttaccga ccagcccatc agcagtacag caaacagggt aataactcgt ttcattacaa
     7561 tcatctcttc gggttcagct gtaggtatag gtgatgctaa tcactgcctg aatggttccc
     7621 tgagtggctc cgccatttac cgtcaatgct ctgacctgta acgggaagtg cgctgattgt
     7681 gaggaatcat ccacctgaac tgttttggtt gcgccactat tcaatgtgtt gccactgtca
     7741 tcctgtagct ctaactggat gttttgcgcg gtcccctggt ttttataata tccggtactg
     7801 tcggctgccc cgctgaagct ggcagtgacc ctcgacgttc ccaccggaca attagtcaac
     7861 tcaagcgcaa catcatgcca ggccgatgcc gccccggcag acataagact gaaagaataa
     7921 agatcgccga gatctaccgt ggcattggtg gtggaaaccg tgcacggttt ggcgacgacc
     7981 ttaccgttca ccgtgatggt gacatcggct gcctgtatcg tcgcacttgc gagcgccaac
     8041 attgccgcca ataaataccc acgtttgcgc catttcatga gcatctccag ttactgatat
     8101 tcaagagtga aggtagccgt ggcattgata tgccccgcag tgacaggcac ctgtgtcgcc
     8161 attagccggg cgtaaaaatt cagcgtattt ggtttacccg gcgtcagggt cgtccacgaa
     8221 agcgcggacg atggggcatt aaggggtatc tgattttgtt gctcattcag aagctgtatt
     8281 cccagtcccg cagccgctga caccgtattt tcaagtgcaa gcaggttggc attgtggcta
     8341 tctgcaacac cggtaaaccc aacttttacg gcagaaacgg cattaccaca gggtgacagc
     8401 aaaatacgaa atggaacgac aggagtcgtc gcgccaatgt tgttaaattg cttcgccgcg
     8461 ttttccatca gatcaacagt aaaattggtt gattcagcgg ccacactaca gccgttatct
     8521 ctgacatagc cgcggatagt aatcgtgcta tccgcagcca aagcgcgact caccgccaac
     8581 cacaaaaaag cgcacagaag ataaaaaggt ttgtttctca tcacgccccc ttaacgacat
     8641 tcagctgata gctgggttaa taactgctgc tgactctctg gtggcagttg ataattggcg
     8701 acacagtgag cattttcctc ttctccccat ttcacctgaa cttttcccgc taaaggcatt
     8761 ccgctgaggt aaacctgacc attatccgca acaatgccgc tactctggct actctctgat
     8821 gtcaccatcg ccccaaacgg cagcggctta ttattgtggg ttagcgtcat gagcagtttt
     8881 atcccaacgc gcgctttaaa ctctgctcgc acgatcgccc cacgagtggg aacaacgtta
     8941 gcgaccgcgt tatctaaatc gacgttatca gccagggtat tggtatccag cgctactctg
     9001 ttttcccgat attcagtggc ataaggcagc acggcataac cgcgccagtc ggtacgcacc
     9061 cccgtctggt tttcgacttt tgcatctttt gcgccaggcg ctttaacaag caccaccgta
     9121 tcgtttaacg gctgccccag cgttacgcca ttggcatgag ccagtacccc accgctgact
     9181 ccgtaataga gctgcttaat atcatcgcta tggctgtaac cgatgttggc attgccgtaa
     9241 ccaccgcgat aattcagcgt ggcgtagcct gtacttccgc tattaccatc acctcccccg
     9301 gcgtaaccgg tttgcacgct atagctgagg ttgttgtctt ccagcaacgt accgtataca
     9361 ccggccagat tggtcatccg accgttgaga tcgtgtgaca tgctgtaact ggcactggca
     9421 tgtcgccact gagatttact gtcagaacgc agccagtggc tgaaaggaat attgacgtta
     9481 aggcagtatg actttc
//
LOCUS       DAZPEP010000152         9401 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZPEP010000152 DAZPEP010000000
VERSION     DAZPEP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.153"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="ACHUT9_003531"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="ACHUT9_003531"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFR1349922.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUT9_003532"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="ACHUT9_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFR1349923.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="ACHUT9_003533"
     CDS             1393..1914
                     /locus_tag="ACHUT9_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1349924.1"
                     /translation="MPTVITHAAVPLCIGLGLSSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSM
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSCYTTPYGHQVIISELMWVWLP
                     GMLLMGMLWWRRR"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUT9_003534"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="ACHUT9_003534"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFR1349925.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUT9_003535"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="ACHUT9_003535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFR1349926.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUT9_003536"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="ACHUT9_003536"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFR1349927.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="ACHUT9_003537"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="ACHUT9_003537"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFR1349928.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="ACHUT9_003538"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="ACHUT9_003538"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFR1349929.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="ACHUT9_003539"
     CDS             complement(6556..7449)
                     /locus_tag="ACHUT9_003539"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFR1349930.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="ACHUT9_003540"
     CDS             complement(7446..8099)
                     /locus_tag="ACHUT9_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFR1349931.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="ACHUT9_003541"
     CDS             complement(8110..9369)
                     /locus_tag="ACHUT9_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFR1349932.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2091 a         2667 c         2494 g         2149 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg ccggaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aaaccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ccgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccga tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa c
//
LOCUS       DAZPEP010000153         9243 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZPEP010000153 DAZPEP010000000
VERSION     DAZPEP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.154"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(152..487)
                     /locus_tag="ACHUT9_003542"
     CDS             complement(152..487)
                     /locus_tag="ACHUT9_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFR1349933.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(491..835)
                     /locus_tag="ACHUT9_003543"
     CDS             complement(491..835)
                     /locus_tag="ACHUT9_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFR1349934.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUT9_003544"
     CDS             complement(837..1010)
                     /gene="ymgI"
                     /locus_tag="ACHUT9_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFR1349935.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUT9_003545"
     CDS             1111..1296
                     /gene="ymgJ"
                     /locus_tag="ACHUT9_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFR1349936.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            1257..1376
                     /locus_tag="ACHUT9_003546"
     CDS             1257..1376
                     /locus_tag="ACHUT9_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1349937.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUT9_003547"
     CDS             complement(1855..2121)
                     /gene="minE"
                     /locus_tag="ACHUT9_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFR1349938.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUT9_003548"
     CDS             complement(2125..2937)
                     /gene="minD"
                     /locus_tag="ACHUT9_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFR1349939.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUT9_003549"
     CDS             complement(2961..3656)
                     /gene="minC"
                     /locus_tag="ACHUT9_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFR1349940.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4176..4544
                     /locus_tag="ACHUT9_003550"
     CDS             4176..4544
                     /locus_tag="ACHUT9_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFR1349941.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUT9_003551"
     CDS             complement(4652..5053)
                     /gene="pliG"
                     /locus_tag="ACHUT9_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFR1349942.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            5295..5588
                     /locus_tag="ACHUT9_003552"
     CDS             5295..5588
                     /locus_tag="ACHUT9_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFR1349943.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            5660..6319
                     /locus_tag="ACHUT9_003553"
     CDS             5660..6319
                     /locus_tag="ACHUT9_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFR1349944.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            6411..6857
                     /locus_tag="ACHUT9_003554"
     CDS             6411..6857
                     /locus_tag="ACHUT9_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFR1349945.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHUT9_003555"
     CDS             complement(7064..7975)
                     /gene="hlyE"
                     /locus_tag="ACHUT9_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFR1349946.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     EYNEKKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFS
                     EKSSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSV
                     QSFFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKE
                     AAKKMINTCNEYQKKHGKKTLFEVPEV"
     gene            8348..8767
                     /locus_tag="ACHUT9_003556"
     CDS             8348..8767
                     /locus_tag="ACHUT9_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFR1349947.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            8767..>9243
                     /gene="umuC"
                     /locus_tag="ACHUT9_003557"
     CDS             8767..>9243
                     /gene="umuC"
                     /locus_tag="ACHUT9_003557"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFR1349948.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQ"
BASE COUNT         2377 a         2050 c         2146 g         2670 t
ORIGIN      
        1 ccggcggcca gtgtctgggg taatgtgggc gtgcagctgg gtgataatgg ctacaatgac
       61 accgcagtga tggtgggcct gaaatataag ttctgatccc cgttagctaa aataccgcgt
      121 cgaattcatc gacgcggtaa catattaaat attatttttt gccgatagca cgcatggtgt
      181 catcaattgc cgtgatcaac agcatttgcg ggtctttagc gcaaacctga ttcagttttt
      241 ctaccacttt ggcgctcagt tccggagatt gctcaatttt taaatcacgg ctggcaacgc
      301 tggcattacc catcaccgca acaatttctg caacctgtgc gctgtcagtt tttgccattt
      361 cgttggcttc tgcgcaagta atataggttt ctgacggcaa accgttttta atattgtagt
      421 cctgcgccca ggtcattggt gcgaaaacaa acaggcccgc cagtaaagca aattttttca
      481 tcatcattcc ttatttcatt ttacccagaa ttgcaccacc cgtaccgcca atcacggcac
      541 ctttaatcgc cccttcgagg ccattgccgg tcagaacgcc agtgacagca ccaacggcgg
      601 cacccacttt tgcaccttta cgtgcatttt taccgtcgcg gcctttttct gttactgcac
      661 caacaccagc gccaacagct gcacctttca gtacgccatt aacaccattg ccagtaagta
      721 atccaacgcc tgcgcctagc aatgcacctt tcgtggtgcg gttcatatcc gccatcgctg
      781 gcgtggagca gaacaatgct gagataagcc cgaaggcaag tatttttttc ttcaacttag
      841 atgtccggta ttaagtaagt tgcacacaca ataatttcgt cttcaattaa gatctgctta
      901 actaaagaac gctcgctatt attcagataa ttcaaaatga gcgtggctgt gatgatagga
      961 attatgtttt ttacgtgaat gagaataatc ttaaatgagg aataactcat tgattggcaa
     1021 tatttttatt caagaagtat cattgactgt taacgcaatg ttgtaaaggt aagataatct
     1081 gatttatcaa tattattgtg tgatttttat gtgagcagaa gatattcatc agcaacgatt
     1141 acattagtca ttttattttg ccgacggcct cattgtcgaa agataagcgt acgacagtat
     1201 tatctgaaaa gagtgatttt ttatccaact acacttcagc gcactgcgtt taaaaaatgc
     1261 ctctttctta tgcgggatat catcatttca tcatgatgtc tttggtgagc ggtgaacaca
     1321 atacacctgc gctgtctctt caggatgaat cccctggtct ggtgcctgcg ggctgatgtt
     1381 gcagcagagc ttaggtcact tagacgctac tatcatttat ccaatggcat ggaatcgaaa
     1441 tcagtcgata cccgcagtat atatcgtgaa ctggtcgttc tgaccatcaa cgattaaact
     1501 gcgcttcggc gcagttttcg tttacagggg gctaaaaggg aaaattctgg ggcaaaaaaa
     1561 gcccgccagt tacggcggga aacctcatcc tatgggagaa caatgaataa tgaaattgcg
     1621 gggttatcat cccccagtgt atccacacta acaataaggt tatttactca gccaggcata
     1681 aacattatgt tctgtgcgtg ggaacagcct taaggtgtaa agggggaggt ggaaatagca
     1741 atgaggagta tcagcaagaa tactcgccgc tttaccacaa cgtggatgag agggatgaaa
     1801 aactcaaggc agaaatgact ctgccttgaa gataaatgcg tttttacagc gggcttattt
     1861 cagctcttct gcttccggta aggtcacgtt cagctcaaga atagaaatat cgccatcttt
     1921 ttgctcaagc tgtacggtta ccatctcagg atcaatttgt acgtatttac aaatgacctc
     1981 aagaatatct ttacgcaact gcggcagata atgcggttct gcatcgctgc gacggcgttc
     2041 agcaacaata atctgcagcc gttcttttgc aatgttggct gtgtttttct tccgcgagag
     2101 aaagaaatcg agtaatgcca taacttatcc tccgaacaag cgtttgagga agcctttctt
     2161 ctcttcttca atgaagcgga aaggacgttc ttctcccaac agacgttcta cggtatctgc
     2221 gtaggcttta cccgcatcgg cgttaatgtc gagaatgacc ggttcacctt ggttagaggc
     2281 gcgcaatact gattgatcct ctgggatcac gccgacgagt ttgatgcgca ggatctccag
     2341 cacatcttcc atgctcagca tgtcacctct gcttacgcgg cctgggttat agcgcgttaa
     2401 cagcaggtgc tctttaatag gctcttcgcc attttctgcg cggcgtgatt tcgacgccag
     2461 aatgcctaaa atacggtcag agtcgcgtac tgaggagact tccgggttgg tggtaataat
     2521 ggcttcgtct gcaaaataga gtgccattaa cgcaccggtt tcaatccctg ccggggagtc
     2581 acaaacgata aattcaaaat ccatcgcttt cagatcatca agaactttgg cgaccccttc
     2641 acgggtgagg gcatctttat cgcgtgtttg cgatgccgga agaatataga gattttcagt
     2701 acgcttatct ttaattaacg cctgatttag cgttgcatcg ccctgaatga cgttgacgaa
     2761 atcgtaaacg acccggcgtt cgcaacccat aatcaggtcg agattacgca ggccgatatc
     2821 aaaatctatc acgacagttt tctttccctt ctgggccaaa ccagtggcga tggccgcgct
     2881 ggaggttgtc ttaccaaccc cccctttgcc cgaagtaaca acaataatgc gtgccataga
     2941 aattccttgt taaaaaggga tcaatttaac ggttgaacgg tcaaagcgtt ttcgactaac
     3001 tgcagtcgcg ccgctttgcc ataaaattct gctgggattt gatcactcag ccagtattca
     3061 cctgcgatgg acaccaattc cgccatcagg ttcgtacaaa atatttgcgt ttcccggtca
     3121 ccacttgccc ctgccagcgc acgaccgcgc atcatgccat agacatgaat gttcccatcg
     3181 gcaatcaatt cggccccagc gctaacgtgg cttgtaacaa tcagatcaca ttgtggagca
     3241 taaatacgct gaccggaacg caccggggta tctattaaac gcgtttttgt gaccggcgtg
     3301 gtattttgcg ctggagcctg tggtgcggga gctggacgtg gcgctttttc ctttccttcc
     3361 gtcaggatag gcagccccat tttttcaatt tcggctttaa gttgcgcgtc tttgcagcca
     3421 cttacgccaa taacccgcaa accggttgcc gaaaccgcct tatgcatcgc tgaccagttt
     3481 accgggtctt ccagtgcact gacgttgagt acaacggggg catgttttaa aaatgcgggg
     3541 gcctgagcga ttttgtcttc cagcgcctga tggataacct taggttctgc ctcatgcaga
     3601 tgaaccacag ataaagtgaa gctactgcct ttaagctcga ttggcgtgtt tgacatcctg
     3661 gccttactca attagctatt aatcatcgcc agcgcgcgat gatgttccga agactataag
     3721 gcatgttata gtctggatta tattgaggca agtcaccctc ccatttattc agagtaaaag
     3781 tctattctgt gataaatggc gctgattcat agcttaaaaa atacccttgt caatcaaccc
     3841 attgccgccg tacttttgat tcttcttatt tacgcttcct ttttccgcac cctaatcgtg
     3901 acagcatctc cagacctgtt taaatatcca gcattgaggc aagtgtattt tttcaccacg
     3961 ccaggctttc ttaactttcc attttttgca gcatcattaa agaacagata cccgttatct
     4021 taatatccgc cacagaattg ctctatcata gaaaccgcag aagacgtctg atacttctgt
     4081 caggtcatcg taactcgtcc aggtcagcgg ttttgattac ggattgatga cgaaatgagc
     4141 ataagtgttg tacggttgga ttatcaggat gtgctatgaa tatgatgcgt attatttata
     4201 tcggattgtc aggtgtgggg atgatgttct catcaatggc gtctggacat gacgctggcg
     4261 gacttcaatc tccggcgtgc ggcgttgttt gcgacccgta tatttgtgtg aactcagatg
     4321 gcatttcccc agagttaaca aggaaatatc tcggcgaaaa agccgctgaa aacttacaat
     4381 cattacaagg ctacgatccc agcgaattta cattcgctaa cggtgtattt tgcgatgtta
     4441 aagaaaaatt atgtcgtgat gatcgttatt ttggtgtaga tggtaagcgg agtggaaaaa
     4501 tcaatcaaac caccacaaag atgttattta tgtgtcgtga gtgatgcctt gtttacatta
     4561 tgttctctgc tattcgtcat taacctcttg agcgacagag aggagtgggg cgttaacgtc
     4621 tatgatgggg atagtccccg tcggttgaca tttacttaat ttgaatatcg acgttatatt
     4681 ttttagtttt gtttttacgg gcgtcgtttc gagtttgcag cactcgcagc tcatatttgc
     4741 cagaagcagg gagtgagtat tgtccgtgac tatcgagttc tggggaatat ctggataaat
     4801 caacagaatc atcgattcct ggcccgaaga gataagtatc agcgccttca ttagagatac
     4861 tcacatgcac tttctgacct tttttggcat agaaggtata tgtatcgtaa tcgtatccct
     4921 ttatttcgcc ggagtactga gcgctgctat ggcctttgct gaactcaaca ttgacatttt
     4981 tacccgcagc aaagctcgtg gaggttagca gtgcaagcag caatacagcc ttactgatgc
     5041 tcttgatttt cattggtagt ctcctgttca gaatgagtac gatgaactgt tataatataa
     5101 caatccctta cgggagaatt ccagtggctt ttctgcgttc ggtaaaatcc cggcgcggca
     5161 ttttttgccg gggggattac ttataaaggg gcatggactc aacaaaccgc agaaatctac
     5221 agctggttgg cttgtggcgc gggtttcatt gatgttagct tatgccaaaa cctggcatct
     5281 taaagagtaa aagtatgttt tgtgtgattt atcgaagcag caagcgtgac cagacctatt
     5341 tatatgtcga aaaaaaagac gatttttcgc gtgttcctga ggaactgatg aaaggttttg
     5401 gtcagcctca gttagcgatg attctgccgt tggatgggcg gaagaaactg gtcaatgctg
     5461 atattgaaaa ggttaaactg gcattaaccg agcaaggtta ctatttgcag ttaccgccac
     5521 cacctgaaga tttgctgaag caacatcttt ccgtcatggg gcagaaaaca gacgacacta
     5581 acaaataacc gatatccggc ggtggcatta tctttgtcgg cgcgggtttt catatccacg
     5641 ataaggtgag gggaacgtta tgtatcaaca tcacaactgg caaggtgcgc tgctggatta
     5701 tccggtgagt aaagtagtct gtgttggcag taactatgcc aaacatatta aagagatggg
     5761 cagcgcagtg cccgaagagc cagtgctgtt tattaaacca gaaacggcac tgtgcgatct
     5821 gcggcagcca ttggcgatcc catccgattt cggttcagtt catcatgaag tcgaactggc
     5881 ggtgttgatt ggcgcgacgc tgcgtcaggc aacggaagag catgtccgca aagccattgc
     5941 cggttacggc gtggcgcttg atctgacatt gcgtgatgtt cagggaaaaa tgaagaaagc
     6001 cgggcagccg tgggaaaagg ctaaagcgtt tgataactct tgtccgcttt ccgggtttat
     6061 ccccgcggcg gaatttaccg gcgatccaca aaatacaacg ctgggcctga gcgtaaacgg
     6121 cgaacaacgc cagcaaggta cgactgcgga catgatccat aaaatcgttc cgttgatcgc
     6181 ttatatgagc aagtttttta ccctcaaggc cggtgacgtt gtgttgacag gcacgcctga
     6241 tggcgtcggc ccgttgcaaa gcggtgatga gctgacagtc actttcgatg ggcattcttt
     6301 gacaactcgc gttttgtaat actttttgcc gcctgaaagc ggcggcaaaa cttgcatcgc
     6361 tgtgccagac tggttataag gtgcgtttta acgtaatggc ggaacacctg atgagcgatg
     6421 tacctttctg gcaaagtaaa accctggacg aaatgagcga tgcggaatgg gagtcgttgt
     6481 gtgatggttg cggtcagtgt tgcctgcata aactgatgga tgaagacacc gacgaaatct
     6541 acttcactaa cgtcgcctgt cgccagctca atattaaaac ttgtcaatgt cggaactacg
     6601 aacgtcgttt cgagtttgaa cccgactgca ttaaattaac ccgtgaaaat ctgccaacat
     6661 tcgaatggct gccaatgatc tgcgcttatc gtttgctggc ggaaggtaaa gatttacctg
     6721 cgtggcatcc gctacttact ggttcgaaag cggcaatgca tggtgaacgt atctctgtgc
     6781 gtcatatcgc agtgaaagaa tcagaagtga ttgactggca ggatcatatc ttaaataaac
     6841 ctgactgggc gcagtgataa atttaataga tgttgatggg ggctttctta atatacgtct
     6901 ggatgctcgc catttcgtag ccctgttttt gaaagcgctc tgtcataatg agagttaccc
     6961 gataccttat tatcaacagg taactctcaa tctttaaaaa acgtctgtgt ttttaaatgt
     7021 gataaacctt ataccttgag tacatggaga gaataatcgc tgatcagact tcaggtacct
     7081 caaagagtgt ctttttaccg tgttttttct gatactcatt acaggtgtta atcatttttt
     7141 tggccgcttc ttttagcaaa gaaagcatta aatcatcata atcaacgtag aatctggtgg
     7201 tttcagtttc cgtttttatc tctccgatgg cggctatttc ggtggttaat ttcaatttgg
     7261 cggcatcgat atctttattc gcttgtttaa ccgtgttaga cagggtggta aagaaactct
     7321 gcacagattt taacttgttc ttcaattctg ggatcagttt cccttcaact acgcccgcag
     7381 caatagaata ggaaatgatt aaaccaaatg gaccggcgac gacaccggct gcggcaccgg
     7441 catacgcttc cttcctgatt ttatctacct gtgactggaa atagctgctt ttttctgaaa
     7501 aatcattggt taactggcta tctaacgcca gcagtttccc ggaagcgttg ttgaaacttt
     7561 gtgagcttac cagcagggat ttttgcgctt cattcagctt cgtgatgccg tcatccagta
     7621 ccttaatgag aatgtctttc tgggcggatg ctttcttctc attgtactca tcaaatagca
     7681 aaatatacgc agcgagcaat tgcgtcgcaa caccacacca ttcatacact gtttgggttg
     7741 cttcaaaata cttatcctgg ctatccataa gtaaggtttt aatatcgccg actaaaacgg
     7801 aggctgcctg tgaatactcc tgtttaaagc gacttaactc ttttatggtt tcatcaaagg
     7861 tctgccaggg gatgacctga tcgagatatt tattataaag atctaatgct ccatctgcgg
     7921 tttcgattgc gtttttaact acttctaccg ttttatctgc aacgatttca gtcataatca
     7981 ttcgcctctt taaatatata aattgtaatg aaactcctgt tttacaacta ttaataaatt
     8041 ttacttcatc taattcatag ttagccgggc gggatgcgtc aatgtcttta tttctattaa
     8101 tatgataaat atcaaacaat gtttaatgtc attatggcga atgcttctat tctatttttt
     8161 tgccgggtga tatttttcat ttctgctgga tgagcgtcgt cgccagaagg ccacgtgagc
     8221 acaggataag agaacgaaaa atcagcagtc tatgcagcga caaatattga tagcctgaat
     8281 cagtattgat ctgctggcaa gaacagatta ctgtatataa aaacagtata acttcaggca
     8341 gattattatg ttgtttatca agcctgcgga tctccgcgaa attgtgactt ttccgctatt
     8401 tagcgatctt gttcagtgtg gctttccttc accggcagca gattacgtcg aacagcgcat
     8461 cgatctgaat caactgttga tccagcatcc cagcgcgact tacttcgtca aagcaagtgg
     8521 tgattctatg attgatggtg gaattagtga cggtgattta ctgattgtcg atagcgctat
     8581 taccgccagc catggtgata ttgtcatcgc tgctgttgac ggcgagttta cggtgaaaaa
     8641 attgcaacta cgcccgacgg tacagcttat tcccatgaac agtgcgtact cgcccattac
     8701 catcagtagc gaagatacgc tggatgtctt tggtgtggtg atccacgtcg ttaaggcgat
     8761 gcgctgatgt ttgctctctg tgatgtaaac gcgttttatg ccagctgtga gacggtgttt
     8821 cgccctgatt tatggggtaa accggtggtt gtgctatcga ataatgacgg ttgcgttatc
     8881 gcccgaaacg ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg gttcaaacaa
     8941 aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca gcaattatga gctttacgca
     9001 gacatgagca atcgggtgat gtcgacgctg gaagaactat cgccccgcgt cgagatttac
     9061 agtattgatg aggcattctg cgatctgaca ggtgtgcgta attgtcgcga tctgactgat
     9121 tttggcagag aaattcgcgc aacggtgcta caacgtaccc atcttactgt tggtatgggg
     9181 atcgcccaga ccaaaacgct ggctaagctt gccaatcatg cggcaaaaaa atggcagcgg
     9241 cag
//
LOCUS       DAZPEP010000154         9169 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZPEP010000154 DAZPEP010000000
VERSION     DAZPEP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.155"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1688
                     /locus_tag="ACHUT9_003558"
     CDS             <1..1688
                     /locus_tag="ACHUT9_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFR1349949.1"
                     /translation="AGLYIKSRTVEAPVETQSTQQTAPDITAVTLPATVSAPPVTPAV
                     VKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTYPANVAQAVQWSHDNPLKQGDAAIQ
                     AVSDQPWDASVKSLVAFPQLMALMGENPQWVQNLGDAFLAQPQDVMDSVQRLRQLAQQ
                     TGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSNTVLTANPVITEPATTVISIEPANP
                     DVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEPFVDSFVRGFGYSMGVATTYALFSS
                     IDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHNGDNINIDVNNFNRITGEHLTDKNM
                     AWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSATQLPAPTRDSQRQAAASQFQQRTH
                     AAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFSRRQPLTQQQKDAARQRYQSASPEQ
                     RQAVREKMQTNPQNQQRREAARERIQSATPEQRQVFKEKVQQRPLNQQQRDNARQRIQ
                     SASPEQRQVFREKVQESRPQRLNDSNHTVRLNNEQRSAVRERLSERGARRQER"
     gene            complement(1759..3183)
                     /gene="lpdA"
                     /locus_tag="ACHUT9_003559"
     CDS             complement(1759..3183)
                     /gene="lpdA"
                     /locus_tag="ACHUT9_003559"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFR1349950.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(3508..5400)
                     /gene="aceF"
                     /locus_tag="ACHUT9_003560"
     CDS             complement(3508..5400)
                     /gene="aceF"
                     /locus_tag="ACHUT9_003560"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFR1349951.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(5415..8078)
                     /gene="aceE"
                     /locus_tag="ACHUT9_003561"
     CDS             complement(5415..8078)
                     /gene="aceE"
                     /locus_tag="ACHUT9_003561"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFR1349952.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(8239..9003)
                     /gene="pdhR"
                     /locus_tag="ACHUT9_003562"
     CDS             complement(8239..9003)
                     /gene="pdhR"
                     /locus_tag="ACHUT9_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFR1349953.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         2096 a         2554 c         2284 g         2235 t
ORIGIN      
        1 ctgccgggct atatataaaa agccgcacag tggaagcgcc tgtggaaacg caatcgacac
       61 aacagactgc gcctgacatc accgcagtta cgcttcctgc aacggtttcc gcaccgcccg
      121 taacgcccgc cgtcgtcaaa tccgcattca gcactgcaca aatcgatcaa tgggttgcgc
      181 ctgtcgcgct gtaccccgat tctctgcttt cacaagtgtt aatggcatca acctatccgg
      241 caaacgttgc tcaagcagtg caatggtcgc acgataatcc acttaaacaa ggcgatgctg
      301 ctattcaggc ggtatctgac cagccgtggg acgccagcgt taaatcactg gtggcctttc
      361 cacaattgat ggcattgatg ggcgaaaacc cgcaatgggt gcaaaacctg ggcgatgctt
      421 ttctggcgca gccgcaggac gtgatggact cggtacagcg attgcggcaa ctggcgcaac
      481 aaaccggctc gctgaagtca tcaaccgaac agaaagttat taccacaacg aagaaagctg
      541 taccggtaaa acagacagtc acggcacccg tcataccatc caataccgtt ttaactgcca
      601 accccgtcat tacagagccg gcaacaaccg tcatttccat tgagcccgcc aatcctgatg
      661 tggtctatat tcccaactac aacccaaccg tggtttacgg gaactgggcc aatactgcgt
      721 atccgccggt ttatctgcca ccaccagccg gagaaccgtt tgttgacagc tttgtgcgcg
      781 gattcggcta tagcatgggc gttgctacca cgtacgcact atttagcagc atcgactggg
      841 atgacgacga tcatgaccat catcatcatg acgatgatga ttatcatcac cacgatggcg
      901 gtcatcgtga cggtaatggc tggcaacaca acggcgacaa catcaatatc gacgtcaaca
      961 atttcaaccg tatcaccggt gagcatctta ctgataagaa tatggcatgg cggcacaatc
     1021 caaactaccg taatggtgtg ccctatcatg atcaggatat ggcaaagcgg tttcatcaaa
     1081 ccgatgtcaa cggcggaatg agtgccacgc agctacctgc tccaacacgc gacagccagc
     1141 gtcaggcggc agcaagtcag tttcagcaac gaacacacgc cgcccccgtc attacacgag
     1201 atacccaacg tcaggcagcg gcacagcggt ttaatgaagc tgaacactat gggagctatg
     1261 acgacttccg cgacttcagc cgtcgccaac cactgaccca gcaacaaaag gacgccgctc
     1321 gtcagcgtta tcaatcggcc tctcctgagc agcgccaggc ggttcgcgag aaaatgcaga
     1381 ctaacccaca gaaccagcag cgaagagagg cagcgcgtga gcgtattcag tctgcaactc
     1441 ccgaacagcg ccaggtgttt aaggaaaaag tacagcagcg cccactgaac caacagcaac
     1501 gtgataacgc ccgccagcgt atacaatcgg catcacctga acaacgtcag gtttttcggg
     1561 aaaaagttca ggagagccgc ccacaacgtc taaacgacag taaccatact gtcaggctga
     1621 ataacgagca acggtcagca gtacgcgaac gtctctctga gcgcggagca aggcgacagg
     1681 aaaggtaaat tgcagacgta aaaaaagcgg cgtggttagc cgctttttta attgccggat
     1741 gttccggcaa acgaaaaatt acttcttctt cgctttcggg ttcggcaggt cggtaatgct
     1801 accttcgaac acttctgccg ccaggcccac agactcgtgc agagtcgggt gcgcgtggat
     1861 ggtcagtgcg atgtcttcag catcacaacc catttcgatt gccagaccga tttcacccag
     1921 cagctcaccg ccgttagtac cgacaatcgc accaccgatc acacggtgag attctttgtc
     1981 gaaaatcagc ttggtcatac cgtctgcgca gtcggaagcg atagcacgac cagaagcagc
     2041 ccacgggaag gtggcggttt catagctgat gcctttctct ttcgcttctt tctcagtcag
     2101 acctacccat gcaacttctg gttcggtata ggcgatagac gggataactt tcggatcgaa
     2161 gtagtgtttt ttaccggcga taacttcagc ggcaacgtga ccttcgtgaa cacctttgtg
     2221 tgccagcatc ggctgaccaa cgatatcgcc gatagcaaag atgtgcggta cgttggtacg
     2281 cagctgtttg tcaacgcgga tgaaaccacg gtcgtccact tccacgccag ctttgcctgc
     2341 gtcgaggttt ttaccgttcg gcacacgacc aatcgctacc agcacggcgt cgtaacgctg
     2401 cggttcagcg ggtgcttttt tgccttccat cgtcacataa ataccgtctt ctttcgcttc
     2461 aacggcggta actttggttt ccagcatcag gttgaatttc ttgctgatac gcttggtgaa
     2521 gactttaacg atgtctttgt cagctgccgg gataacctgg tcgaacattt caaccacgtc
     2581 aatctgtgaa cccagcgcgt ggtatacggt gcccatttcc agaccgatga taccgccacc
     2641 cattaccagc aggcgttctg gtacttcttt cagttccagc gcgtcagtgg agtcccagat
     2701 acgcggatct tcatgcggaa taaacggcag ttggatcggg cgagaacccg ctgcaatgat
     2761 cgcattgtcg aagttgatca cggttttgcc gttctcacct tcaacttcca gggtgttagc
     2821 cccggtgaat ttacccagac cgttgaccac tttgactttg cggcctttcg ccataccagc
     2881 cagaccaccg gtcagctgat tgatcacttt ctctttccaa gtacgaatct tgtcaatatc
     2941 ggttttcggt tcgccgaaga cgataccgtg ttcagccagc gctttggctt cttcgataac
     3001 ttttgctacg tgcagcagtg ctttagaagg gatacagccg acgttcaggc aaacaccgcc
     3061 aagggtgttg taacgttcta cgattacggt ttccagacct aaatcagcgc aacggaaggc
     3121 agcggagtaa cctgcggggc ctgccccaag taccacgacc tgagttttga tttcagtact
     3181 catcatgacc tctatatatt tatctccggc ggtcataccc gtcgtctttc aggccgcagc
     3241 ggcgttagct tcacgccctc accccagtca cttacttatg taagctcctg gggattctgg
     3301 cgcttgctac cttgctgcac ccagaaatcc atagggtatg tgaagtaata ccctaaccac
     3361 caccgggtcg ttctatccgt cggtatttta caaaattgtt aacaattttt aaacaacaaa
     3421 cggcaaccga tttgtctatt cgctaataac ctcaatacat tcatgagatt accagaaaaa
     3481 agccggccgt tgggccggct cttttactta catcaccaga cggcgaatgt cagacagcgt
     3541 gttgttaatg atggtaatga aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa
     3601 ggagagagaa atcggcagca tcagacgcgg cacgaactct ttaccattcc acaccggctc
     3661 catcgcggac ttggaaacgc cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa
     3721 gtgggtagta cccaggccgc cgatgctgga gatggtgaag caaccgccct gcatttcgcc
     3781 cgcagtcagc ttaccgtcac gcgctttctt agaaatagtc atcagctcgc gagacagctc
     3841 gatgatgcct ttcttgttga cgtctttgaa taccggaaca accagaccgt tcggggtatc
     3901 caccgccaca ccgatgttga tgtatttctt cagggtcaga cgctgaccgt cttccgacag
     3961 cgaactattg aagcgaggca tctgctcaag agctgcagca acggctttca tgatgaagac
     4021 aaccggggtg agcttcacat ccagcttacg tttcgccgct tcttcgttct gctgtttacg
     4081 gaacgcttcc agctcggtga tatcggtttt gtcgaagtgg gtaacatgcg ggatcatcac
     4141 ccagttacgg ctcaggttag caccagaaat tttctggata cggcccagtt ccacttcttc
     4201 gatttcgcca aacttgctga agtccacctt cggccacggc agcatgccag ggataccacc
     4261 gccagtcgcc gccggagctg cttctgcacg tttgatagct tctttcacgt aagcctgaac
     4321 gtcttcgcgc aggatacgac ctttacgacc agtgcccttc actttcgcca ggttaacgcc
     4381 aaactcgcgt gccagacggc ggatcagcgg agtcgcgtga acgtaagcgt cgttttcagc
     4441 aaattcagat ttgccttcgg ctttcgcagc cggagctgct gccggggctt cagctttcgc
     4501 tgccggagcc ggcgctgccg cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc
     4561 aacttcgaag atcataatca gcgagccagt tttcacttta tcgccaacgt tgactttcag
     4621 ttccttcacg acgcctgcaa acggagccgg gacctccata gaagctttat cgccttctac
     4681 ggtgataagc gattgttcag cggcaacttt gtcgcccact ttcaccatca cttcggtcac
     4741 ttcaacttcg tcaccgccga tatccggaac gttaacttct ttcacgccag ccgctgatgc
     4801 aggggccgct gccggagctg cttcctgttt agccgccgga gctgccgcgc ctgcttcacc
     4861 cgcgacttcg aagaccataa tcagcgagcc ggtagacact ttgtcaccca cgttcacttt
     4921 gatctctttc acggtgccag caaacggagc cggaacttcc atagaagctt tatcgccttc
     4981 tacggtgatc agcgactgtt cagcttcaac tttatcgcca actttcacca ggatttcggt
     5041 cacttcaact tcgtcgctgc cgatatccgg aacgttaacg tcttttgccg ccgcagccgc
     5101 gggtgctgct gccggagctg cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc
     5161 tgcaccgtcg gcggaatcga aaatcataat cagtgcgccg gtctgggttt tatcgccaac
     5221 agagactttg atctctttaa cgatacccgc ctgcggagac ggaacttcca tagaggcttt
     5281 gtcgccttct acggtgatca gcgactgttc ggcttcaact ttgtcgccca ctttgaccag
     5341 gatctcggtg atttcaactt catcagcccc gatgtccggt actttgattt cgatagccat
     5401 tattctttta cctcttacgc cagacgcggg ttaactttat ctgcatcgat gttgaatttg
     5461 gcgattgcgt cagcaaccac tttcttatcg atttcgccac gtttagccag ttcgcccagc
     5521 gccgcaacca cgacataaga agcatcaact tcgaagtggt gacgcaggtt ctcacggctg
     5581 tcggaacgac cgaagccatc agtacccagt acgcggtagt cgtcagccgg tacgtaagta
     5641 cggacctgct cagcgaacag tttcatatag tcggtagatg ccactgccgg agcgtcgttc
     5701 atcacctgag cgatatacgg aacgcgcgga gtttccagcg gatgaagcat gttccagcgt
     5761 tcacaatcct gaccatcacg cgccagctcg gtgaaggagg tcacgctata aacgtcagaa
     5821 cctacgccgt aatctttcgc caggatctct gccgcttcac ggacgtgacg caggatagaa
     5881 ccggagccca gcagctgaac tttacctttg ctaccttcaa tggtttcgag tttgtagata
     5941 cctttacgga taccttcctc agcaccttcc ggcattgccg gcatgtggta gttttcgttc
     6001 agcgtagtga tgtagtagta aacgttctct tgtttttcac cgtacatacg ctccagaccg
     6061 tcatgcatga tgacagcaac ttcgtaagcg taagccgggt cgtaagagat acagttcggg
     6121 atagtcagcg actgaatgtg gctgtgacca tcttcgtgct gcagaccttc accgttcagg
     6181 gtggtacgac cggaagtacc gccgatcagg aagccacgcg cttgctggtc gccagccgcc
     6241 cagcacagat cgccgatacg ctggaagccg aacatcgagt aatagatgta gaacggaatc
     6301 atcggcagat tgttggtgct gtaagaggtc gccgctgcca gccaggaaca ccctgcgccc
     6361 agctcgttga tcccttcctg cagaatctga cctttctcgt cttctttata gtaagcaacc
     6421 tgctcgcggt cctgcgggat gtactgctga ccgttcgggc tgtaaatacc aatctgacgg
     6481 aacagacctt ccataccgaa agtacgcgct tcgtcggcga tgatcggtac cagacgatct
     6541 ttgatcgact tgttcttcag cattacgttc agggcacgaa cgaaagcgat agtggtagag
     6601 atctctttgc tctgctcttc cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc
     6661 ttctcggtga agttcggctg acggcttggc agataaccgt gcagtttctg acgctgtgcg
     6721 tgcagatagg tatgctcttc agaaccttcc gggaaggtga tgtacggcag tttttcgata
     6781 tctgcatcag acaccggcac attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc
     6841 attttcttaa cctggtgcgc gatgttttta ccttcagccg cgtcgcccat gccgtaacct
     6901 ttaatggtat gagcaaggat tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat
     6961 gcagcgtaga ttttcttcgg atcgtgacca ccacggttca gtgcccagat ctgctcgtca
     7021 gtccagtctg caaccagtgc tgcggtttca ggatatttac cgaagaagtg ttcacgaacg
     7081 tacgcaccat ctttcgattt gaaggtctgg tagtcgccgt caacggtttc gttcatcagc
     7141 tggatcagtt taccgctggt atctttacgc agcagttcat cccaacggct accccacatc
     7201 actttgataa cgttccagcc agcaccttcg aagatgcctt ccagttcgtt gatgatcttg
     7261 ccgttaccgg tgaccgggcc gtcaagacgc tgcaggttac agttgataac gaagaccagg
     7321 ttatccagtt tttcacgggt agcgatggtg atcgcacctt tggattccgg ttcgtccatt
     7381 tcaccgtcgc cgaggaacgc gtaaacggtc tgtttagagg tatctttcag gccacggtgt
     7441 tccagatatt tcaggaattt agcctggtaa atagcaccaa tcggccccag acccatagat
     7501 acggtcggga actgccagaa ttccggcatc agtttcgggt gcggatagga agagaggcca
     7561 ttgccgtgaa cttcctgacg gaagttatcc agctgctctt gagtcagacg accttccagg
     7621 aaagcacgag cgtacacgcc cggggagatg tggccctgga agtaaaccag gtcgccgcca
     7681 tcctgctcgt tgcgtgcacg gaagaagtgg ttaaagcaca catcataaat ggttgcggaa
     7741 gactggaagg acgccatatg gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc
     7801 gtcatgatgg cgttccagcg gatagctgaa cgaatacggc gttccagttc cagattaccc
     7861 ggatactccg gttgttcttc aacggggatg gtgttgatgt agttgctgat acctgtgcct
     7921 gcggctacgt ttacaccgcc tttgcgggct tcagcaagca gttggtcgat cagatactga
     7981 gcacgctcaa caccttcttc acggatgacc gattcgatcg cctggagcca gtcgcgagtt
     8041 tcgatcggat ccacgtcatt tgggaaacgt tctgacatgg gttattcctt atctatctaa
     8101 taacgttgag ttttctggaa cctgtcccat tgaactctcg ccggaaagct caataagaca
     8161 ggttctacgt ttagttgccg cgcttttata tacgcttgat ttacaacatc ttctggataa
     8221 tttttaccag aaaaatcact aattctttcg ttgctccaga cgacgcagag aacgctcacg
     8281 gcggctctct tcacgacttc tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg
     8341 cgatgcttcg cgcgcttctt ccggcttacc ggccataatc gcttcaaata tgcgggtgcg
     8401 gtgactactc accagcggca gcatctcgcg acgcgaatag agcaattcga agttctggcg
     8461 gacattctgg gccaacatcg gctccataca ccttagcaga tgaagcagaa ccacattgtg
     8521 ggccgcttcg gtgacggcaa tctgatactg gagtacggcg tttgattccg cgtccagatc
     8581 gccagactgc tgcgccagct ctatggcgtg gtggagttca cggatgcgtt ccttgtcttc
     8641 atcggtacta cgcagcgcgg cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc
     8701 gagcaagtca tactgtgatt caggatggtc ggagagcagc tccaccagcg gatcgctgaa
     8761 gctttgccat aggctgctct ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc
     8821 cttcgcttcg agacgttgaa tcgcctcacg caaggaggga cgggagacgt cgaactgttt
     8881 tgccagttcg cgttccggtg ggagtttttc gcccgggcgg agagtgcctt cgagaatcaa
     8941 aaactccagt tgctgctcaa tcacatcgga gagttttggt tggcggattt tgctgtaggc
     9001 catgagttcc tgtcttaagc cacttgccga agtcaattgg tcttaccaat ttcatgtctg
     9061 tgacgctaaa gtaacaaagt attcacctta tgtccataca ggttttgatt gaaatcatga
     9121 aactgtgcac attttaacaa cttgacatat ataacgtttc aaaattgta
//
LOCUS       DAZPEP010000155         9074 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZPEP010000155 DAZPEP010000000
VERSION     DAZPEP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9074
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.156"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(505..1272)
                     /gene="fecE"
                     /locus_tag="ACHUT9_003563"
     CDS             complement(505..1272)
                     /gene="fecE"
                     /locus_tag="ACHUT9_003563"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFR1349954.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1273..2229)
                     /gene="fecD"
                     /locus_tag="ACHUT9_003564"
     CDS             complement(1273..2229)
                     /gene="fecD"
                     /locus_tag="ACHUT9_003564"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFR1349955.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2226..3224)
                     /gene="fecC"
                     /locus_tag="ACHUT9_003565"
     CDS             complement(2226..3224)
                     /gene="fecC"
                     /locus_tag="ACHUT9_003565"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFR1349956.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3221..4123)
                     /gene="fecB"
                     /locus_tag="ACHUT9_003566"
     CDS             complement(3221..4123)
                     /gene="fecB"
                     /locus_tag="ACHUT9_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFR1349957.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4168..6492)
                     /gene="fecA"
                     /locus_tag="ACHUT9_003567"
     CDS             complement(4168..6492)
                     /gene="fecA"
                     /locus_tag="ACHUT9_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFR1349958.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6579..7532)
                     /gene="fecR"
                     /locus_tag="ACHUT9_003568"
     CDS             complement(6579..7532)
                     /gene="fecR"
                     /locus_tag="ACHUT9_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFR1349959.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7529..8050)
                     /gene="fecI"
                     /locus_tag="ACHUT9_003569"
     CDS             complement(7529..8050)
                     /gene="fecI"
                     /locus_tag="ACHUT9_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFR1349960.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8187..8255
                     /gene="ykiE"
                     /locus_tag="ACHUT9_003570"
     CDS             8187..8255
                     /gene="ykiE"
                     /locus_tag="ACHUT9_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFR1349961.1"
                     /translation="MGWSLCFWHVSVRMTHDVVCYY"
     gene            8342..9039
                     /locus_tag="ACHUT9_003571"
                     /pseudo
     CDS             8342..9039
                     /locus_tag="ACHUT9_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1F family transposase"
BASE COUNT         1988 a         2510 c         2585 g         1991 t
ORIGIN      
        1 actttttaga aaaatcgatg gtcagaaact ggattagcaa ttccgttcca gggttgcttt
       61 tgatttacgt tggcgtctga tcattgattt atcctcaaaa gcccaacctc attggtaatg
      121 aaccagctcc gtgaatgtcc gctctggcac agagcgaaat tttttgatct cccccccctg
      181 aaatctaaac ttagtcatgt cacgtttttg ggtttctaaa attttaactt cgcgtttttc
      241 gttgccgtaa gggttataca gaaatgtccg ttaagcagag ttcaaaattg attgccgtga
      301 tcacgactgg tttgaaagcc gcgcccaagc ctgtacagct ctggtttgcg ttgattatga
      361 acctgtcagc ctaaagcaag cggatggacg atgagtattg gtaatctttc agagtccgga
      421 aaagttcagc cccagtctga acaggcttgc tggcgccagt ccagtttcat tcagtcgtgg
      481 tttggttctt acggcctgtg caatctacct cattaggcac atcggcctgc cagataccgg
      541 ctcggggtgt atttccgctt ccacgctgaa tactgttctc agcaatcctg gggtcatcac
      601 ctcttctggt gtgccttgcg ccataacatg tccgtttgcc attaccacca gttgatcgca
      661 gtaccggcta gcctgattaa ggtcgtgcag cacagcgacc accgttttcc cctgagtccg
      721 gagttcgccc atcaaccgca tcaggtccac ctggtgattg atatcaagat aggtggttgg
      781 ctcatcaagt aatacaacgg gcgtattctg ggccaggacc attgccagaa atgcgcgctg
      841 gcgctgaccg ccggaaagct cggttaaccg acgaacggca agatgattga tccgggtctg
      901 gttcatggcg acattaactc gtgcattgtc ttcagcggag agacgccccc agagtgacag
      961 ccagggatta cgaccatacg aaaccagctc ctggactgtg atcccctctg gcgttaaatg
     1021 gtgctgaggc agcagcgaaa gcctgcgggc caactggcgc gatgagagca tatttatggg
     1081 attatcgccg agaaatacgg tgccagactg cggcattaaa agccgcgaaa aacagtttaa
     1141 cagcgtcgat ttcccgcaac cgttaggacc gatcagggcg gtgatcttcc ccgttggcag
     1201 tgagagtgaa acgtcgttaa gtaccttgtc tgtcccgtaa ttgaccgtca gattttcagt
     1261 tcgtaaagtc atttatcgca ttctcacaag caaccagaca aaccacggcg caccgataat
     1321 ggcggtcagc acgccaaccg ggagctccag tgggggatga ataattctcg ccagcagatc
     1381 ggcaaccacc aacagcaacg cacctgtcag ggccgaaaca ggcagcagtc tgcggtgacg
     1441 tccaccggtg atgctacgca tcatatgcgg caccacgaga ccaataaagc taatcgggcc
     1501 gcaggcggcc acgccggtag atgtcatggc gacagctagt aacaaagccc agaatcgggt
     1561 atagggcacc gacacaccga gcgtggtggc gcgcgcatcg ccgagtgcaa ggaggtcgag
     1621 atcgcggcaa aaactcaggc tcagcggcag aaataaaatc atcagcggga tggcaatctt
     1681 cacaaagctc cagtcacggc cccataagct gccggtcagc cacagcaggg cgttgttcac
     1741 atcctgcggg cgcgagagca tcagataatc cgtcaggctg gcccagcatg cagaaagcgc
     1801 cacgccggtg agtgccagct tcatcggctg gtgggtcttt gccagcatct tcagcaatat
     1861 caaccccgcc atgccgcccg caaaggccag cagcggcagc accatcacgg gcagtgacgg
     1921 cataagaagt agagccccca cagaggccag gctggcggca tggttaacgc cgagaatatc
     1981 cggtgatgcc agagggttgc gcacaatccc ctgtatcagc acgcccgcca cggcgagggc
     2041 tgcaccgaca aacagtgcca gcagcaagcg cggcagtcgg tactccatca atacataata
     2101 atgctcgcgt ccggcctgcc agtcggtcag cagcgcgcgc cacggcacgg ggatcactcc
     2161 catatggagt gataacagcg cacagcccgc cagggcaagg gtgatgaaaa taaccagcgc
     2221 aattttcatc ctcgcctcct cacaagccag acaaagcaag ggctgccaat cagcgccagc
     2281 actgcgcctg cgggcagatc tccggggaag gccagcgcgc gtgcgagtac atctgccagc
     2341 agcatcagcg tggcccccag cagcatgctc actggcagta cgttgcgctg atcgaagcct
     2401 gcccagaagc gcgccagatg tggcaccagc agaccgataa acgccaccgg acctgccacg
     2461 ctgacgcacg caccaaccag aagcagcact aacatattga tgaccaaacg tagcctcgtc
     2521 aggttcactc ccagcgtatg ggcggtgctg tcgctgaggt tgagcaggtt cagttgattc
     2581 gccagcagca acacgacagg gactgcagtg accaccaccg gcaagagctg ccagacatcc
     2641 tgccagcggg cgtgggacac tccgccagcc agccagtaaa agatgccgta agcatgatct
     2701 tcggccagca gcagggtgat gcgggtaagg cccatacaaa aggccgacag cgcgataccc
     2761 gcgaggatca gtttgtttct gtcatgggta tgacgaaatc cgcctcctgc ggtcatgacc
     2821 agcagccagc tcacgccgcc cccgcatgcc gcgatgaacg acagagaata gcctgcaatc
     2881 ggcgtcggac tcagcgcgct ggtaagcgcc atagccagcg ccgcgccgct gttaatgccg
     2941 agcagtgaag gagaggccat tgggttgtgg gtcagggttt gcagcagcgt gcccgcgagc
     3001 gccaggcttg cgccgatcag aacggcgacc aggcttcgtg gcaaacgaag gttttgcacc
     3061 agcgcttctg gtagcgttgg cgtgtgtcca ggcagcaggg cgcgggttgc atctgctccg
     3121 gaaacaggaa tggccgagta gcaaaacaga ctcagccaga aaataataat aagtgctgca
     3181 acgggaagcc cccacagcag caccgggtgt tttatcgcgg tcatttcaca acggtaagcg
     3241 gctgatggtg gaagattttt accgtgtcag cggcaatacg ctctgcagca aaaataccgc
     3301 gcatccgcgc ccaggtgtta ctgtcgaccg aagcaacctg ctgcttctgc gcggcggtta
     3361 acatctgcca gagcggatct tgttgccagc gtttaacaat gctctcttcg cgatagtggg
     3421 caaccagcag ccaggcagga ttgaccgcca gcagttgctc caggccgatg gacggcatgg
     3481 acgcacccgc catcgcagcg ggaacgttca gccccagaga ggccagcacg ctgccggtcc
     3541 aggtctcctg agtatgcagg ttgaattgct gttcgcgtga tgtgccaaag gccacgcgtg
     3601 tccctttggg aagctggctg gcccactgcg ccatcctctc tttatgttgt tccagacgtg
     3661 cctgcatctc tcgcttttta cccaccattt cgccgatgat agccgcagat tgcaaatttt
     3721 cagcgtaggt ttcgttgcgg gacttaagca gcagtaccga cgcgatttgc tgcaaggcga
     3781 tgtaaacccc cgcatggcga ctgctgtcgg caatgatcag gtctggtttc agagcggcaa
     3841 tggcttccag gctcggctgc gcgcgcgttc cgacggactg ccacggtttc aggtgcgcac
     3901 gcacttcggg caggatgcgt tttgcatcgt tatcgtcggc aataccgatc gggctgacgt
     3961 ccacggcggc cagcgcatcg gcgaacgaga gttccagcac cacaatccgt tgtggcgttt
     4021 tttcgagtgt aaacgtgccg tgttcgtcct gaaccgtggc ggcaaaggcg tggctgatca
     4081 ccagcagcag gcctgcaaaa agaaaacgga taaatgccaa cataatcaca ttccagctaa
     4141 aagcccggca agccgggcgt taacacatca gaacttcaac gccccctgca tatacagcgt
     4201 gcgcggctgg cctgcataga tgcctttgtt gttgtcgtca taagagcgga tgaagtagtc
     4261 ctggtcgaag atgtttttca caccgaatgc caggttcaga tctgccatct gcgggccaaa
     4321 gtcatacacc acgcgtgcgc cccagagcat gaagccggga atgcggccgg tactgccgtc
     4381 ggcgctctct ttcaccgtat tggcgttatc cgcaaactgg ctggactgga aatcgctgtt
     4441 cagattgaac atccagtttc ctggcttgta gtccacgccc agcgtgcctt tatgtttcgg
     4501 ggagaatggt accagattgc cgtaggtgtc gcctttctcg cggatttccg cgttcacata
     4561 cgcatagctg gcgtagatgg aaacgttatc aagcgttggc gttagcgtac ccagatcgta
     4621 acgtgcctgc gtttccagcc cggtatggcg cgttttgcca cgtgcagtga cggtgtcgtt
     4681 ggtctggttg gagtcgtact gattgttaaa gttaatcagg aacagcccca tttccgccgt
     4741 cagcgcgccg tcgtcgtagc gggtaccgag ttcccaggtt cgcgcttttt ccggttccac
     4801 attgccgctt tgcacagcct tgccaatctg gctgtactgt acggtgccga acgagccttc
     4861 agtgtttgca taaagattcc agctgtcagt caggtgatag agcacgttca acgccggaag
     4921 cggtgcgtta tagctcactt cttcgtgcgt gcctgtgatg gcgttgttct ggtatgactc
     4981 gatatgttcg aaacgcatac ccggcgtgat ggtccagttg ccgatgtcga ttttgtcatc
     5041 cagataccag gcgtgcgcct cggtgccgga acgcgtatcg cggtcgtaag ggcttgagcc
     5101 ggacggcaac tgcccgctgc tggtggcggt gtagtaacgc atttcatgcg ttgattcatt
     5161 cacatagcga tagcccacgc ccacttcgtg cgcggaaggg ccgatcataa agatctggct
     5221 gtagcgtggc tcaataccgc gcacccagta gttacgcggc gagagggtga tgcgtttgcc
     5281 ttgctccagg tagccgctgc gcagggtttg ggtgtagaac ccctgaatgt tgaatttatg
     5341 ctggctgtct ggctggaact ggtagcccag gctcgccagc ttgcgacgac cccagaagcg
     5401 gtcatacggg cgggtggatt gccagcgatc ggcgtcgtaa tccgcgcgag acaggccacc
     5461 gggcatgtcg gcttcaccgt cgtaatattg cagcaggctg ttgaaggtgt gcacctcatc
     5521 cggcgcatat ttgcttttca gcatcaggtc gtcgatgcgg gtggcgctgt gctcgcgcca
     5581 gtcactgccg cgcgtgccgg agtagagcag cgcggtgcca aaaccgttgt ccgctgtgcc
     5641 gcccaccatc aggttgtgcg tctctttcgg gttgttttgt gaagaggttg ggctgagctg
     5701 accttccacg cccgcctcga taccaaagtc ctgcggaatg gcacgggtaa caaagttcac
     5761 cacgccgccc acgctctgcg gtccgtaacg caccgcacca ccaccgcgta ccacgtcaat
     5821 ggcatccatg ttgccgagcg aaacgggagc cagtgaaagc tgcggctgac cgtaaggggc
     5881 gaaggggacg gggatgccgt ccatcaggac ggtcgagcgg ctggcgaggc gcgggttcag
     5941 gccccggatg ccaaagttca tcgccaggtc gtggctgccg gtgccgttgt tttccggcgc
     6001 gctgacgcca gggatgcggt taagtacctc acgcatggtg gttgcgccgg ttttggcgaa
     6061 atcctcacgg cggatcacgt cacgcgcgcc agcatgttca aatacgtcgt tttcacgcgc
     6121 atcacccagc cagtcgccga ccacggtcag ggcatcttct tttggtgcgg gcgcgggctc
     6181 cagcgtccag ctgttatttc ccagcggttt tacctgcagt ccgctgccgt ccagcagttg
     6241 ttgcaggccg ctctcgacgt cgtaatcgcc gtgcaggccg ttgctctgct tgccgcgcgt
     6301 caggctggcg tcaaccgaga gggtaaatcc gctgtgtgcg gcatactgat tgagcgcttt
     6361 atcgagcgat cccggtgcga tattaacctg tgcagcaaaa gcggaaaacg agagaccggc
     6421 cagcggcagc aggctcaggc gaatggcgtt aaccaaaggt gttgttttac gaaaaacgcg
     6481 taacggcgtc ataccttccc catcatcatt tttgttgtgt tcagctatga gtcgaacgag
     6541 aagggtaaaa aggacaatcg aaataagaat tattttcctt acagtggtga aatgtttatc
     6601 cagtaccgcg taatagactg aattttaacg ggaagcgttt gcgcgataac gttcaggatc
     6661 gcatcggtat ttttcagcgg gaacgtcccg ctcaggcgca gcccggcaac ggcgggatcg
     6721 cagcgcagca cgccgttgcg gtaacgggtt agcgtggcta tcacctcacc cagcggttta
     6781 tcgctgaagc tcaggatgtc cttcgtccag cttgtactct cgtcatccag cggtttcact
     6841 gcgccaaact cagaggcgct gaactgcagg ctttcaccag cgttcacgat gcgtttttgc
     6901 gcgggggcac tggcgagaag cacttccacg gcgtgctgct gcacgtcaag ctgcgtgaaa
     6961 ttatcctgct ggcggacggt aaattctgtc cctaaagcag tgagctggcc ctgacgggtc
     7021 aggacacgga aggggcgttg cagggcatct ttcgcggtag taatggcgat ttcaccgtac
     7081 cagagccgga cggtgcgctg atgcgcatca aaacgcatat ccgccgcgct ttgggtattc
     7141 agcgtgagca gggagccatc ttccagttgc tgacggctga ctgtgccttt ggcggtgcgg
     7201 taatctgccc gcagaccttc gccggtttcc gactgccaga gctgccagcc tccgccagcg
     7261 ccgagcaaca gcagtaatcc tttcatcacg tgacggcggg tgaggcgggt atcgtgcaac
     7321 gcccggctgg caacgtcgcc aggcacaccg ccaagctggt tgcgcaggtt ttcaacctgc
     7381 tgccaggccc actggttatc ctgatcctgt tcataccact gttgccagcg cgcttcctgt
     7441 tgtgggctga cgcgctcgcc gcttagcacg gcataccagt gggaagctga acgcagcgcc
     7501 tgacggcggg aatcggttaa caaaggattc ataacccata ctccagacgg aacagcaggc
     7561 agtgctcgac ggctttcgcc acgtattttt tcacggagct gatggaaaca ccgagtttgt
     7621 gcgcaatctc gctgtatgtc agaccatcca gttgcgaaag cagaaacgct tcacgtgttt
     7681 tgccgtttag cccgtccagc atgctgtcga ggagttgtag ggtctcgagt tggctttcgc
     7741 gttcctcagg tgaaggcgct cccccctccg gcataagcgc cagcatctcc agatacgctt
     7801 tttccagcgc gtttcggcga aacaggtcca ccatcacgcg tttggcgata gtgcagagga
     7861 aggagcgagg atcgcggatc gtcgagagcg tttcgctgac cattacccgc aaaaaagtgt
     7921 cctgggcaat gtcatctgca tcaaaagcag actggagttt gcgcgtcagc cagcttttca
     7981 accagccgtg atgtgtgcca taaagcgact cgaatgttaa ggaagctgtg gtagtggcgc
     8041 ggtcagacat gcggagtgca tcaaaagtta attatcacgt agccatatta atatgagaat
     8101 agttatcatt acaattggaa ataaaattgt ttccaataga catttttaac atgttgtttt
     8161 tctaagtgtt ataaggtagg tataaaatgg gatggagcct ctgcttctgg catgtgtcgg
     8221 tcagaatgac tcatgatgtg gtctgctatt attgacatcc tcactgccct aaaggatagg
     8281 gatttcggta atgctgtcaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg
     8341 cgtggcttcc atttccatca gatgtccttc ctgctccgct actgaaggcg tggtgcgtaa
     8401 cggcaaaagc actgccggac atcagcgcta tctctgctct cattgccgta aaacatggca
     8461 actacagttc acttacaccg cctctcagcc cggtaagcac cagaaaatca ttgatatggc
     8521 catgaatggc gtcggatgtc gcgccagtgc acgcattatg ggcgttggcc tcaacacggt
     8581 tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca accgggcagt
     8641 gatgtgattg tctgcgctga aatggacgaa cagtggggct acgtcggtgc taaatcacgt
     8701 cagcgctggc tgttttacgc gtatgacagg atacggagga cggttgtggc gcacgtcttc
     8761 ggtgaacgca ctctggccac actggagcgt cttctgagcc tgctgtcggc ctttgaggtc
     8821 gtggtataga tgacggatgg ctggccgctg tatgaatcac gcctgaaggg aaagctgcac
     8881 gttatcagca agcgttacac tcagcgcatt gagcgacata atctgaatct gagacaacat
     8941 ctggcaaggc tgggacggaa gtcactgtcg ttctcaaaat cggtggagct gcatgacaag
     9001 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat tacctcctcc
     9061 cgaactcaaa acta
//
LOCUS       DAZPEP010000156         8840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZPEP010000156 DAZPEP010000000
VERSION     DAZPEP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.157"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHUT9_003572"
     CDS             complement(<1..1782)
                     /gene="atoS"
                     /locus_tag="ACHUT9_003572"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFR1349962.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATF"
     gene            1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHUT9_003573"
     CDS             1949..4798
                     /gene="rcsC"
                     /locus_tag="ACHUT9_003573"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFR1349963.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHUT9_003574"
     CDS             complement(4998..5648)
                     /gene="rcsB"
                     /locus_tag="ACHUT9_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFR1349964.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHUT9_003575"
     CDS             complement(5665..8337)
                     /gene="rcsD"
                     /locus_tag="ACHUT9_003575"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFR1349965.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         2168 a         2050 c         2260 g         2362 t
ORIGIN      
        1 gaaggttgcg ccgtagcccg gcaaactggc gacgcgaata tcaccctgat gggcattaat
       61 gatgcgttga cttaacgcca gaccaagccc ggttcctgag gctttggtgg tgaaaaaggg
      121 atcgaagatc tttttttgca gcgagagatc aatgccacag ccgttgtcct ctatcgaaat
      181 ggcctgttgt gagtcgctgt attgccaggt ttgaatgcga attttccctc gtgcgctgat
      241 agcctggacg gcattgatca ggatattcag tagtacctgt ttgagcagtt cacgatcggc
      301 gttaatcggg cttaattcat tatccagttc gcttatgaag tcgacccgcg cttgtacgcc
      361 ggcggtttgt accagtacca gagtttcttc aaccaatgca ttgaggctga cttgttgcca
      421 ttgactgtgg cgtggacgtg aaaattcgag caattgctga ataactttgt taattgaatc
      481 gatttctttg agtactacgg acagatattc ctgatgtatt gggtcactgg tttgttggcg
      541 caagatctgt acataaccac gaatagccgt taacggatta cgtacttcat gcgcgacgcc
      601 agccatcagc tcacccagtg tggcgaggcg ttctgcttgc gccatgcggc gctgggtttc
      661 tttgcgggca gttaaatcag agaaaatcac caaagcacct atcatttcac cgtgcgtgtt
      721 atgaatacga ctggtagtga cactgagttc aatggtgcgg tcacgacctg gaaaactgat
      781 ctccagcgcc acatgttcgg tgccatgttc cagcgtatcc agtactggac tgtagaactg
      841 agtattgtcg aacaacatgg agtaaggctg ccctaccagt tcatggcgtt gatagccagt
      901 gataacttct gctgctgggt tcatggtggt tacatcaccc tggcggtcaa tggcaatgac
      961 gccatcggca gcgttttcaa taatcagatc gttaagtgtc cgcgtttcac gcagtgcctg
     1021 ggcgaggtta ttaacactct gactgatttg ccccatttca ccgggcaatt gtggtaatcg
     1081 agtgggaata ttttgtgcca gagtcgagag gccatcggtg atgatatcaa tattggcgct
     1141 caggcgacgg gagaaaagga caatcaatag caggcttatc agcaaatcgg cggtgagcac
     1201 aatgataatc ctcacatcca ttttccaggc ctggcggcga atatcttcgg ttaattcatt
     1261 ggcccagata tagccgagga tttcaccatt acgctcaatg gggagcattg aattcaaaat
     1321 atcgccgcgc acctgcctgc ctgaataaac caaaggggta tttgtacgca tgacttcgcg
     1381 accagggtga tctgcggcaa tggtaacgcc gacattattc tgatatagcg ctgaaggcgc
     1441 gtaggtgatt atcgcatcca gcattttgtt gtaataacca gcacctatgc cagggaaggc
     1501 gtgagtgata ttttcggtaa tgggggcaag ttctgcattt aatgcgcgga tacgctcctc
     1561 acgtggtaag tcgatgtaga gatcatagcg atcgcctagt gcctgattaa gcaggttgac
     1621 cacggcagaa agttttttct ctttttcaga taagactgct gaacgtcctt ccgtttctac
     1681 gatataacca atagtaagcg ttgggacaat gaccatcagg attgccatca ggatcatttg
     1741 attgcgtaag cggcgtggat aaatccactt catataatgc atgctgaaat ccactagtct
     1801 tgtccggtat atgacgatta tcagaggtta aggtgatgat ttctcggcgg tgtatcatat
     1861 tccagagaag agagaacatt gcggtaacac gcttttaccg ctaccttaac cacactccat
     1921 cggtcacctg aggcggagct tcgccccttt gaaatacctt gcttcttttc gtacaaccct
     1981 gaaagcctcg cgctacatgt tcagagcatt ggcgttagtg ctctggctgt tgattgcttt
     2041 ttcatccgtt ttttacatcg ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga
     2101 atttaatctg agttccgatc aggctcagcg ctttattcaa cgcacctctg atgtgatgaa
     2161 agagctgaag tacatcgccg aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg
     2221 tggacgagaa acgcaggcgg atgtgcctgc gtttgaaccg ctgtttgccg actccgattg
     2281 ttccgcaatg agtaacacct ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta
     2341 ctggcgcgat aatttttctg cggcttacga tctaaaccgg gtatttttaa tcggcagcga
     2401 taacctctgc atggccaatt tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt
     2461 gaaagctttg catgaacgca tcaataaata tcgaaatgca ccacaagatg atagcggcag
     2521 taacctctac tggatcagcg aaggtccgcg ccctggcgtc gggtattttt acgcgttgac
     2581 gccagtttat ctggcgaacc ggttgcaggc gcttttgggt gtcgagcaga ccatccggat
     2641 ggagaacttt ttcttaccgg gtacgttgcc gatgggggtt accattcttg atgaaaatgg
     2701 tcataccctg atttcgctta ccggaccaga aagtaaaatt aagggcgatc ctcgctggat
     2761 gcaggaacgc tcctggtttg gctatacgga agggttccgg gagctggtgc tgaagaaaaa
     2821 tctgccaccc tcatcgctaa gcatcgtgta ttcggtgccg gttgataagg tgctggaacg
     2881 cattcgcatg ttgatcctta acgcaatttt gctgaatgtg cttgccggag ctgcattgtt
     2941 tactctcgca cggatgtacg agcgacgtat tttcattccg gcggaaagcg acgccctgcg
     3001 actggaagaa catgagcagt tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg
     3061 cattttgcgt accgctgatg gcgtcaatat tttaagtaac gaactggcgc atacctatct
     3121 caatatgctt acgcatgagg accgccaacg actgacgcaa attatctgtg ggcagcaggt
     3181 caattttgtt gatgtcctga ccagcaacaa taccaatctg caaatcagct tcgtccattc
     3241 gcgctatcgt aatgaaaacg tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa
     3301 gatggaagag tcgttgcagg agatggcaca agcagcggaa caggcgagcc agtcaaaatc
     3361 gatgttcctt gccaccgtca gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa
     3421 cctggatctg ttgcaaacca aagagttacc gaaaggcgtc gatcggctgg tgacggcaat
     3481 gaacaactct tccagcctgt tgttgaaaat tatcagcgat attctcgatt tctcgaagat
     3541 tgaatcggaa cagttgaaga tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca
     3601 catcaccgcc aactatttac cgctggtggt acgcaagcag ttaggcttgt actgctttat
     3661 tgaaccggat gtgccagtgg ccttaaatgg cgacccgatg cgtttacagc aggtcatctc
     3721 caacctgttg agtaacgcca taaaattcac cgataccggc tgtatagttt tgcatgtccg
     3781 cgctgatggc gattatctct ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa
     3841 agaagtggtg cgcttgtttg atcccttctt ccaggtcgga acgggcgtac agcgaaactt
     3901 ccaggggacc ggtctgggtc tggcgatttg tgaaaaactg atcagcatga tggacggcga
     3961 tatctcggta gattcagaac cgggaatggg cagccagttt accgtgcgta ttccgttgta
     4021 cggcgctcag tacccgcaga aaaaaggcgt ggaagggttg agtggtaaac gctgctggct
     4081 ggcggtccgc aatgcgtcgc tctgtcagtt cctggaaacc agtttgcagc gcagcggcat
     4141 cgtcgttaca acatacgaag ggcaggaacc gactcccgaa gatgtgttaa ttactgacga
     4201 ggtagtgagt aaaaaatggc agggtagagc ggtagtgacc ttctgtcgtc gccatattgg
     4261 tattccgctg gagaaagcgc caggggagtg ggtacacagt gtggctgccc cgcatgagct
     4321 accggcattg ttggcgcgta tttatttgat cgagatggag agcgacgatc ctgctaacgc
     4381 tctgccgtcg acggacaaag cggtcagcga taatgacgat atgatgattc tggtcgtgga
     4441 tgatcatccg attaaccgac gtctgctggc agatcagttg ggatcgttgg gctatcaatg
     4501 taaaaccgcg aatgatggtg tcgatgcgct taatgtactt agcaagaatc atattgatat
     4561 cgtgcttagc gacgtcaaca tgccaaatat ggatggttac cgcttgacgc aacgcattcg
     4621 tcagttggga ctgacgttgc cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa
     4681 gcagcggtgt ctggagtccg gtatggacag ctgcctgtcg aagccggtaa cgctggatgt
     4741 gataaaacag acgctgacgg tatatgccga gagggtcagg aaatcgcggg aatcgtagga
     4801 cggataaggt gtttatgccg catctggcat tcagtgcaaa tgccagatgc gacgctgacg
     4861 cgtcttatct ggcctacttt gatgtaatct gttgaaataa tgggaatcgt aggacgcata
     4921 aggcgtttac gccgcattcg gcaatcggtg caaatgccag ataagacact aacgcgtctt
     4981 atctggccta caggtgatta gtctttatct gccggactta acgtcactga agagagataa
     5041 ttcagcaggg cgatatcgtt ctcgacaccc agcttcatca tcgcagattt cttctggcta
     5101 ctgatggttt taatactgcg gttcagcttt ttagcgatct cggtcaccag gaagccttcc
     5161 gcaaacaggc gcagaacttc actctctttt ggcgagagac gcttgtcacc gtaaccacca
     5221 gcactgattt tttccaacag gcgagaaacg ctttccgggg taaatttctt ccctttctgc
     5281 agcgcggcga gagctttcgg cagatcggtc ggtgcacctt gtttcagcac gatcccttcg
     5341 atatccagat ccaataccgc actaagaatc gccgggttgt tgttcatagt cagaacaatg
     5401 atcgacaggc ttgggaaatg gcgcttgatg tacttgatta aggtaatgcc atcgccgtac
     5461 ttatcgccag gcatggagag atcggtaatc aacacatgcg catccagttt cggcaggttg
     5521 ttgatcagtg ctgtagagtc ttcaaattcg ccgacaacat tcacccactc aatttgctca
     5581 agtgatttgc gaataccgaa caagactatc ggatggtcat cggcaataat tacgttcata
     5641 ttgttcatgt aataggctac cttgctacag caagctcttg acataactgt caatgtcgct
     5701 gatgtatttt tctattcctg gaacatcctt ctcacgaatc agatgttcca gcgtttcaca
     5761 taactgcttg ccgggtacca gatttagcat ggcaaatacg cctttaagac gatgagccgt
     5821 ttgggctaac gcagcaaagt cactggttgc tgcttcagta tacagcctct taacatcatc
     5881 cggtactgtg tctacaaaga gcgcataata gccgctggca tggagttgcg cattttcatc
     5941 tccgcccaga ggcgattctg tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa
     6001 gaccgcttcc tgcatagcgt tgctcatatt gaagttgacg cacaattgac caggcccaat
     6061 ttcccgtacg ccagactcat catcgcttaa aagcaagcca gaggcagtaa gattagacgg
     6121 attatccgtt aaaaagatat cataatcttg actaattaat ctttcatcgg gtgtgataca
     6181 ggttgcaccc caattttcta actggcgagt gacaatattc cgaatttctg ccgaagtaac
     6241 atccaccatt acgcagacat catccagtaa acgctcttct tcctcttcaa cttccgggcc
     6301 agcggcgagc attttgatat gcacagagta gcgtgtacca agcccatccc gcgttttgat
     6361 gtttaaatga ccgcccagtt tacgtgccag ttgatcgctc agccagaatg ccagcgggtc
     6421 cgcctttcca taacgatcgt tttgggtctg gttgataaac gggaagtgca aattatccat
     6481 ttcatgaata cttacgcctt ctccggtgtc cagaatgcgg aatgtcaggc ggtcttcaga
     6541 ggactcatcc tgatcaacct caagggtgat ttttcccaat tgcgttgagg tcacggcata
     6601 ttgcatcagt agcagcaaaa tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg
     6661 tgctttcaga tgattgttaa tcagcagttg cagaccttta cgcttgatgg caggcaacac
     6721 tgaaggcaca acttcatcaa ttaaatcctg cacggagaac agcaccgtct cacttttcca
     6781 gctatcgtcc gcaagcatgt tcgctaactg tatttcatcg accagccgca ccagcacatc
     6841 tgcctgattc gccagttgtt tgctttccgg ggcgttgagt ttagccgcgc tctccgccag
     6901 ggactgtgca ggttctttca gcgcatcgcc aatgtttttc ataaaggtca tccggccctg
     6961 ctggtttttc tcatacagac gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg
     7021 atcctgatcg cgaataatga aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg
     7081 gatctcatac agctcgttat tgatcgtcgc ctgaataatc ccctgatgct gttccgccat
     7141 ggtggtgatg ttttgcagat tcaaatgcgg cagcaaatga tcggcaattt tgttacttat
     7201 gacagtgcgg ttcgattcct gatcgtgaac cagcaggccg agcggcagca gtgagactat
     7261 ctcttcattg attgcccgta aaatgcgcaa ttcgttattg accgccgtgt tgggtagact
     7321 ttctgtactg cgactggaga aatggcggaa tgtggtatag ccaaataacg ccagcgccag
     7381 caaaccgatg ttcagcagca gtggcagcag aatgttttgc aacgtatcca gcaataaggt
     7441 gccataagga acctgccaga ccaggcgcat atcggtagag ttgagtgccg aggagatttc
     7501 aatcttcgta ctgttaaagt ggatactgac gctatccgtt ccttctttct cattatcatt
     7561 gtctcccgtt gccgtcgtgt ctggctcaag gcggaaactg tccagcggca tacccggtgg
     7621 gatcaaatca ttaatcggca gatcaaaagc cacgaccgtt gccagatgtc ccggctggtt
     7681 gaacgtggta cgcaaggtaa agtaatgacc gttctgccag gccaggcggc gcatgttaga
     7741 aaaactttcg cgttcatcga gggcgttggc ctgttgcaac atttctgcac gacgtgaatc
     7801 aacaatgtca ctgacggtcg attctttaaa tccggaggtg agatctttga ggggcagggt
     7861 tgagatcagt accagactgt tatcctgacc attcaggtaa tacatcgacc acggtacatt
     7921 ttctgcaccc cacaatgtat ccagataagt ggacatccgc tgagtcatct caagcgttga
     7981 gttgtcgtga gagccaaaga ttaacgcttc cgttttgcgg cgtggtttct ccagatagta
     8041 gacatcctgt ttcaggcgcg tctcttgtaa accttcgccg gaggaggggg aggtcgtcgc
     8101 ggcaatgttg tcgtagatct gccaggtcac gtaacgccag gtatcgacgc gcttttggat
     8161 tgcgtgggta atgtcgacaa tctggtaact tttatctttc agccaggcgt taacggcgct
     8221 ttgtaccatt acccccatcg tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt
     8281 aatgctcccc ggtaggagtg aaaagcgggt cgtggccgtt gtctctttct gacgcattcg
     8341 tgtttatgac ctgttaaaac ttcgcgaagt gatagggcag tataaagggt acagtgtgaa
     8401 attccagact cttacctgat tgatgattct ctattctccc gtgatattta tcatcctgca
     8461 ttgggggaaa tagatgtaca agcgccattt tgctgtacaa ataacctaca aaaagcccaa
     8521 cgaaattaat tgcaaataaa cgaagattgc tggaaattat gctgatgtta attatttgtg
     8581 aaatagttga caagagttat agtttttctg tggtagcaca gaataatgaa agtgtgtaaa
     8641 gaagggtaaa aaaaaccgga tgcgaggcat ccggttgaaa taggggtaaa cagacattca
     8701 gaaatgaatg acggtaataa ataaagttaa tgatgatagc gggagatatt ctagttgcga
     8761 gtgaaggttt tgttttgaca ttcagtgctg tcaaatactt aagaataagt tattgatttt
     8821 aaccttgaat tattattgct
//
LOCUS       DAZPEP010000157         8790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZPEP010000157 DAZPEP010000000
VERSION     DAZPEP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.158"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="ACHUT9_003576"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="ACHUT9_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFR1349966.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUT9_003577"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="ACHUT9_003577"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFR1349967.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="ACHUT9_003578"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="ACHUT9_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUT9_003579"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="ACHUT9_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFR1349968.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUT9_003580"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="ACHUT9_003580"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFR1349969.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQVLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="ACHUT9_003581"
     CDS             complement(6490..6846)
                     /locus_tag="ACHUT9_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFR1349970.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="ACHUT9_003582"
     CDS             complement(6850..7266)
                     /locus_tag="ACHUT9_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFR1349971.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUT9_003583"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="ACHUT9_003583"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFR1349972.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8732
                     /locus_tag="ACHUT9_003584"
                     /pseudo
     CDS             8492..8732
                     /locus_tag="ACHUT9_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2240 a         2171 c         2138 g         2241 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aatcccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccagg tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgccaaag atttttctta tgctaaagat ttctttttat acatagaaac
     8641 acagttaaaa attgcaaaag atttttgtag acctggagaa gaagtatcaa gttctattgc
     8701 aagtaaagtt tttcacgcat ttatcgattt agttaacaaa atcaggggta agaaagactt
     8761 tatgtatatt tgcacgcttt gctgttttgc
//
LOCUS       DAZPEP010000158         8696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZPEP010000158 DAZPEP010000000
VERSION     DAZPEP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.159"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..134
                     /gene="yhaV"
                     /locus_tag="ACHUT9_003585"
     CDS             <1..134
                     /gene="yhaV"
                     /locus_tag="ACHUT9_003585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1349973.1"
                     /translation="NDENTLRTYGKKTNAYTVFSKMLKRGHPPADWETLTRETEETH"
     gene            complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHUT9_003586"
     CDS             complement(189..998)
                     /gene="agaR"
                     /locus_tag="ACHUT9_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFR1349974.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHUT9_003587"
     CDS             1247..2527
                     /gene="kbaZ"
                     /locus_tag="ACHUT9_003587"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFR1349975.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCNMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGDAHETLSELAVTTPDAARATLEAHRHAFKKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            2550..3023
                     /gene="agaV"
                     /locus_tag="ACHUT9_003588"
     CDS             2550..3023
                     /gene="agaV"
                     /locus_tag="ACHUT9_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFR1349976.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            3034..3813
                     /gene="agaW"
                     /locus_tag="ACHUT9_003589"
     CDS             3034..3813
                     /gene="agaW"
                     /locus_tag="ACHUT9_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFR1349977.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPSAQKEEFEDGI"
     gene            3803..4681
                     /gene="agaE"
                     /locus_tag="ACHUT9_003590"
     CDS             3803..4681
                     /gene="agaE"
                     /locus_tag="ACHUT9_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFR1349978.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            4699..5133
                     /gene="agaF"
                     /locus_tag="ACHUT9_003591"
     CDS             4699..5133
                     /gene="agaF"
                     /locus_tag="ACHUT9_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFR1349979.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTCLVDELGRCHEECPVEEGI"
     gene            5130..6263
                     /gene="nagA"
                     /locus_tag="ACHUT9_003592"
     CDS             5130..6263
                     /gene="nagA"
                     /locus_tag="ACHUT9_003592"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1349980.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLCITDGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVFDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAAAWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            6614..7768
                     /locus_tag="ACHUT9_003593"
     CDS             6614..7768
                     /locus_tag="ACHUT9_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFR1349981.1"
                     /translation="MPENYTPAAAATDTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYTPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHUT9_003594"
     CDS             7781..8641
                     /gene="kbaY"
                     /locus_tag="ACHUT9_003594"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFR1349982.1"
                     /translation="MSIISTKYLLQDAQANDYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KCFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         2005 a         2325 c         2315 g         2051 t
ORIGIN      
        1 tgaacgatga aaacactctg cgcacctacg gtaaaaaaac aaatgcctat accgtattca
       61 gcaaaatgtt aaaaagagga catcctcctg ccgactggga aaccctcacc cgagagacag
      121 aagaaaccca ttgatggtgt tcacaatgcc cgccgaacgc attcgtgttg accagcgggc
      181 accctttctc actccccgac cagaatcact tcaaccccag cctttcgcag tccttccaga
      241 ctatccgcag gaatgccttc atcaacaatg atcatgtcga tacgttgagt atcaatgatc
      301 ttatgtaaac tggagcggtt gaacttactg gaatcggtga ccacgatgat ccgttccgca
      361 acttcgcaca tccggcggtt taaacgggct tcatcttcat tatgtgtgct gacgccgcgc
      421 tccagatcga tcgcatctac accaagaaac agcatatcga agtggtaatt ttgcagcgat
      481 tgctcagcct gatcgccgta aaaagattgc gactgacggc gcaaatgccc gccggtcatc
      541 agcagctcaa cgccttcggc ttccagcaac gcattagcca cgttcatacc gttggtcatc
      601 gcaattacgt cagtgtgctt gcgcatcaga cgagcaatct caaaagtggt ggtcccggaa
      661 tcgaggatca cccgatgacc tggctgaatc aactcaacgg cagctttcgc aacgctgcgt
      721 ttcatcgcgg tgttcagtgc gcttttatct tccactgatg gctcgactga cggcgtcgtg
      781 ctatcgcaga tcaacgcgcc accataggca cgcacagcga tcccctgctt ttccagaaac
      841 gccagatcgt tgcggatcgt cacagtagat acgccataca atgccgacag atcgttaacc
      901 tgcacactcc cttgctgtcg caggcgctga atgatctgtt ctcgtcgctc gctggtgcct
      961 gtcactcgct tctcacctga agcgtcggta ttactcatag taagtccttt cgtaaaactt
     1021 tcgtttcatt tcgttttgcc tattaacgcc tttctattaa gcaaatgcaa gcccaccttg
     1081 cccattgacg caagctactc tcgtttcagt aactttcatt atgtttcttt tgtgaatcag
     1141 atcagaaaac tattatcttt cgttttattt ttatctcacc atggcgcagt atcaactgaa
     1201 acaaaacgaa agattaatat cgcagtaatc tgaactggag aggaaagtga aacatctgac
     1261 agaaatggtg agacagcaca aagcgggcaa aacaaatgga atttatgccg tttgttccgc
     1321 acatccgctg gtgctggagg ctgcaatccg ctacgccagt gcaaaccaaa cgccgttact
     1381 gattgaagca acctccaatc aggtagacca gttcggcggt tataccggaa tgacgcccgc
     1441 cgattttcgc ggctttgttt gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt
     1501 gattctgggt ggtgaccatc tggggccaaa ccgctggcaa aacctgccag ccgctcaggc
     1561 aatggccaat gccgatgatt tgattaaaag ctacgttgcg gcaggattca aaaaaatcca
     1621 ccttgattgc aacatgtcct gtcaggacga tccaattccc ttaactgatg acatcgtggc
     1681 tgaacgcgcc gcccgtctgg cgaaagtggc ggaagaaacc tgtcgcgaac actttggcga
     1741 agccgatctg gagtatgtca ttggtaccga agtgccggta cctggcgacg cgcatgaaac
     1801 cttaagcgag ctggcggtca ccacgccgga tgccgcccgc gccacgctgg aagcccatcg
     1861 tcacgccttt aaaaagcagg gtctgaatgc catctggcca cgcatcattg ccctggtggt
     1921 tcaacccggc gtcgaattcg atcacaccaa cgttattgat tatcagcccg ccaaagcgac
     1981 cgccttaagc cagatggtcg aaaactacga aacgctgata ttcgaagcgc actctacaga
     2041 ttatcaaacg ccgcaatcgc tgcgccagct ggtgattgat cactttgcca ttctgaaagt
     2101 tggcccggcg ctgaccttcg ccctgcgtga agccctgttt tctctggcgg cgattgaaga
     2161 agaactggtg ccagcaaaag cctgttccgg tctgcgtcag gtgctggaaa acgtgatgct
     2221 cgaccggccg gaatactggc aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc
     2281 gcgtggttat agctactcgg atcgcgtgcg ctattactgg ccggacagcc agattgatga
     2341 cgctttcgct catctggttc gtaatctggc ggattcacca attccgctac cgctcatcag
     2401 ccagtatctg ccgctgcaat acgtgaaagt tcgctccggc gagctgcaac cgacgccacg
     2461 ggaactcatt atcaaccata ttcaggacat cctggcgcag taccacacag cctgtgaagg
     2521 ccaataagca aaacaaagag gaacacgcta tgccaaatat tgttttaagc cggattgatg
     2581 aacgcttgat tcacggtcag gtcggcgttc agtgggtcgg atttgcgggg gcaaatctgg
     2641 tgctggtagc caacgatgag gttgccgaag atccggtaca acaaaacctg atggaaatgg
     2701 tactggcaga agggatcgcc gtacgtttct ggacgctgca aaaagttatc gacaacattc
     2761 atcgcgccgc cgatcgacag aaaatcctgc tggtttgtaa aacacccgcc gatttcctga
     2821 cgctggtgaa aggtggcgtt ccggtgaatc gcattaacgt tggcaatatg cactacgcca
     2881 atggcaaaca acaaatcgcc aaaacggttt ctgtggatgc gggcgatatc gcagcattta
     2941 acgacctgaa agccgctggg gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg
     3001 tggacctctt taaattactt tgagggattc atcatggaaa tcagtctgtt gcaggcattc
     3061 gcgttgggca ttatcgcctt tatcgctggc ctggatatgt ttaacggcct aacccatatg
     3121 caccgcccgg tggtcctcgg cccgttggtc gggctggtac ttggcgatct gcataccgga
     3181 attttaaccg gcggtacgct ggaactggtg tggatggggc tggccccgct ggcgggcgca
     3241 cagccgccta acgtgattat cggtactatc gtcggcacgg cgtttgccat tactactggc
     3301 gtgaaacccg atgtcgcagt cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg
     3361 attaccttcc tgttctcggt gatgtctggc gtgatgtctc gctgcgaccg gatggcggag
     3421 aacgccgata ctcgcggcat tgaacgcgtg aactatctgg cactgctggc actcggcact
     3481 ttctattttc tctgcgcttt cctgcctatc tacttcggcg cggaacatgc aaaaaccatc
     3541 attgatgtcc tgccgcaacg attaatcgac ggcctcggcg tcgcgggcgg catcatgcca
     3601 gcaatcggtt ttgccgtgct gctgaaaatc atgatgaaaa acgtctacat cccctacttc
     3661 atcctgggct ttgttgccgc agcctggctc aagttaccgg tgctggctat tgctgccgct
     3721 gcgctggcga tggcgctgat cgacctgctg cgtaaatctc ctgaaccgac tcaaccttcg
     3781 gcacagaaag aggaatttga agatggcatc taatcaaacc accctgccga acgtcagtga
     3841 aaacgaagaa acattgctga ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg
     3901 cgcggagctg acgaaaaaag atatcaaccg cgtcgcctgg cgttccatgt tgttacaagc
     3961 gtcttttaac tacgaacgta tgcaggcttc tggctggttg tacggtctgc tgcctgctct
     4021 gaaaaagatc cacactaata aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt
     4081 cttcaatacc catccgtttc tggtgacgtt tgttatcggc attatccttg cgatggagcg
     4141 ttctaagcag gacgttaaca gtattcagag caccaaaatt gccgtcggtg cgccgcttgg
     4201 cgggattggc gatgcaatgt tctggctgac gctactgccg atttgtggcg ggataggcgc
     4261 aagcctcgcg ctacaaggct ctattcttgg cgcagtcgtc tttattgtgc tgttcaacgt
     4321 ggtacacctg gggctgcgtt ttggtctggc gcattatgct taccgcatgg gcgtggcggc
     4381 gattccactc attaaagcga ataccaaaaa agtcggccat gctgcgtcaa tcgttggaat
     4441 gacggtaatc ggcgcactgg tggcaaccta tgttcgttta agcaccacgc tggaaatcac
     4501 agcgggcgac gcagtggtta agttacagac tgatgttatt gacaaactga tgcctgcctt
     4561 cttaccgctg gtctacaccc tgaccatgtt ctggctggta cgccgcggct ggagtccgct
     4621 gcgcctgatt gcagtgaccg tggttctcgg catcgtcggt aaattctgcc atttccttta
     4681 aatacaatga ggtattagat gttaagtatt attttgacag ggcatggcgg gtttgccagt
     4741 ggcatggaaa aagcaatgaa gcaaatcctc ggcgatcaat cgcagtttat tgccatcgat
     4801 tttcccgaaa cctcaagcac cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa
     4861 ctggattgtg aagacggcat tgtttttctg acggatttgc tgggcggcac gccgtttcgc
     4921 gtagcttcaa cgctggcgat gcaaaaaccg ggctgtgaag tgattaccgg caccaatttg
     4981 caactgctac tggagatggt gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg
     5041 caggcgctgg agtgtggaca ccgtgggctg acctgtctgg tggacgagtt aggccgctgc
     5101 catgaggaat gtccggtcga ggaaggaata tgacacacgt tctgcgcgcc agaaggctgc
     5161 tgactgaaga gggatggctc gatgaccatc agttgtgtat tactgacggt gtcatcgcag
     5221 caatcgaacc gattccagtg ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt
     5281 acatcgacac ccatgtacac ggtggtgcgg gcgttgatgt aatggatgac gcgccggatg
     5341 tgttcgacaa gctggcaatg cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca
     5401 ccgtaaccgc gccgcttaat accattcatg cggcgctgaa acgtattgct caacgttgcc
     5461 aacgcggcgg acctggtgcg caagtgctgg ggagttatct cgaaggaccg tacttcacgc
     5521 cgcagaataa aggcgcgcat ccgccggagt tgtttcgcga gcttgaaatt gccgagctgg
     5581 atcagttgat tgccgtttct cagcacacct tacgtgtggt agcgctggca ccggaaaaag
     5641 aaggggcatt gcaggccatc cgccatctta aacagcaaaa cgtaagggtg atgctggggc
     5701 atagcgcggc ggcctggcaa caaacccgcg ccgcatttga tgctggtgcc gacggcctgg
     5761 tgcattgcta taacgggatg acaggtttac atcaccgcga accgggaatg gttggcgcgg
     5821 gattaacgga caagcgcgcc tggctggaac tgatagccga tggtcatcat gtgcatccgg
     5881 cggcaatgtc gctgtgttgt tgctgtgcga aagagagaat cgtactgatc accgacgcga
     5941 tgcaggccgc tgggatgccg gatggtcgct atacgttatg tggtgaagaa gtgcagatgc
     6001 acggtggcgt tgtccgtacc gcgtccggtg ggctggcggg cagtacgcta tctgtcgatg
     6061 cagcagtgcg caacatggtc gagttaacgg gcgtaacgcc tgcggaagcc atccatatgg
     6121 cgtcgctgca tccggcgcga atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg
     6181 gcaaacgcgc cagcgtcgtt gcgctggata gcgggctgca tgtgcaacaa atctggattc
     6241 agggtcaatt agcttcgttt tgatggtttg ctccattatt gggccttcac ttcccccgta
     6301 aggcctttct ttttctttcg ttttgatctg tgcagcggtg ccggatgcga cgctaacgcg
     6361 tctgatccga cctacggtac gccgcatccg gcaatcggtg aacatagcga cgtcattacg
     6421 caattcttta atcttccctt gtcttttctt tcaacgatca cagatttcgt tttatttccc
     6481 ttccctccat tgaactttcg gtttcttttc tatagatttt aatcaacgaa agacatcacc
     6541 aagtgaaatg aaacgaaagg caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac
     6601 taaggattga gttatgccag aaaattacac ccctgctgcc gccgcaaccg atacatggac
     6661 tgaagaagag atccgccatc agcctcgcgc atggatccgt tcactcacca acatcgacgc
     6721 gctacgttcc gcgctcaata acttccttga accgttactg cgcaaagaga atctgcggat
     6781 catcctgacc ggagccggaa cgtcggcatt tatcggtgac atcatcgcgc cgtggctcgc
     6841 cagccatacc ggtaaaaact tcagcgccgt accgaccacc gatctggtca ccaatccgat
     6901 ggactacctg aatccagctc atccgctgct gttgatctcc ttcggtcgat ccggcaacag
     6961 cccggaaagc gtcgccgccg tggaactggc aaatcaattt gtacccgagt gctatcacct
     7021 gccgatcacc tgcaacgaag cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc
     7081 gtttgccctg ctgatgcccg cagaaacgca cgatcgcggc tttgcgatga ccagcagcat
     7141 taccaccatg atggccagct gcctcgcggt tttcgcacct gagacgatca acagccaaac
     7201 cttccgcgat gtggcggatc gttgccaggc gatcctgacc tcactgggtg atttcagcga
     7261 aggtgtgttt ggttatacac cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca
     7321 gggcgcagca cgcgagtcgg cgctgaaagt gctggaactg actgcgggta aactggcggc
     7381 cttctatgat tccccaaccg gattccgtca tggcccgaaa tcgctggtcg ataacgaaac
     7441 gctggtggtg gtatttgtct caagccaccc ttacacccgt cagtatgatc ttgatctgct
     7501 ggctgaactt cgccgtgaca accaggcaat gcgcgtaatc gccatcgccg cggaaagcac
     7561 cgacgttatc gccgctggcc cgcatatcat cctgccgcca tcccgtcact ttatcgacgt
     7621 tgagcaggca ttttgcttcc tgatgtacgc ccagacgttt gcactgatgc agtcgctgca
     7681 catgggcaat acgccggata ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg
     7741 cgtaatcatt catccgtggc aggcataaga ggatcgcatt atgagcatta tctccaccaa
     7801 atatctgtta caggacgccc aggccaatga ctacgcggtg cctgccttta acattcataa
     7861 cgccgagacg atccaggcga tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct
     7921 cgccggaacg ccggggacct ttaaacatat cgcgctggaa gagatctacg ccctgtgtag
     7981 cgcctattcc acaacctaca acatgccact ggcgctgcat ctcgaccacc acgaatcgct
     8041 ggatgatatt cgccgtaaag tccacgcagg cgtgcgcagt gcgatgatcg acggcagcca
     8101 cttcccgttt gccgagaacg taaagctggt gaaatcggtt gtcgacttct gccactccca
     8161 ggattgcagc gtggaagcag aactgggccg cctgggcggt gttgaagatg acatgagcgt
     8221 tgacgccgaa agtgcattcc tgaccgatcc acaagaagct aaatgctttg tcgaactgac
     8281 tggcgtcgac agcctggcgg tagcgattgg tacggcgcac ggcttataca gcaaaacgcc
     8341 gaagattgat ttccagcggc tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct
     8401 gcatggtgcc agcgatgttc cggatgaatt tgtccgtcgc actattgaac ttggcgtcac
     8461 aaaagtgaac gttgccacag aattaaaaat agccttcgct ggcgcggtta aagcctggtt
     8521 tgcggaaaat ccgcagggta atgatcctcg ttattatatg cgcgtcggaa tggatgcgat
     8581 gaaagaagtt gtcagaaata aaattaatgt ctgtggttca gcgaatcgaa tttcagcata
     8641 atcattagtt ttttatgatt tatcccaatg tacttcccga ttaatatcgg ggagtg
//
LOCUS       DAZPEP010000159         8680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZPEP010000159 DAZPEP010000000
VERSION     DAZPEP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8680
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.160"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(279..542)
                     /gene="rpsT"
                     /locus_tag="ACHUT9_003595"
     CDS             complement(279..542)
                     /gene="rpsT"
                     /locus_tag="ACHUT9_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFR1349983.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            645..863
                     /locus_tag="ACHUT9_003596"
     CDS             645..863
                     /locus_tag="ACHUT9_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFR1349984.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            871..1812
                     /gene="ribF"
                     /locus_tag="ACHUT9_003597"
     CDS             871..1812
                     /gene="ribF"
                     /locus_tag="ACHUT9_003597"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFR1349985.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1855..4671
                     /gene="ileS"
                     /locus_tag="ACHUT9_003598"
     CDS             1855..4671
                     /gene="ileS"
                     /locus_tag="ACHUT9_003598"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFR1349986.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4671..5165
                     /gene="lspA"
                     /locus_tag="ACHUT9_003599"
     CDS             4671..5165
                     /gene="lspA"
                     /locus_tag="ACHUT9_003599"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFR1349987.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5290..5739
                     /gene="fkpB"
                     /locus_tag="ACHUT9_003600"
     CDS             5290..5739
                     /gene="fkpB"
                     /locus_tag="ACHUT9_003600"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFR1349988.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5741..6691
                     /gene="ispH"
                     /locus_tag="ACHUT9_003601"
                     /gene_synonym="lytB"
     CDS             5741..6691
                     /gene="ispH"
                     /locus_tag="ACHUT9_003601"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFR1349989.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6757..7671
                     /gene="rihC"
                     /locus_tag="ACHUT9_003602"
     CDS             6757..7671
                     /gene="rihC"
                     /locus_tag="ACHUT9_003602"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFR1349990.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7838..8659
                     /gene="dapB"
                     /locus_tag="ACHUT9_003603"
     CDS             7838..8659
                     /gene="dapB"
                     /locus_tag="ACHUT9_003603"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFR1349991.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1980 a         2165 c         2464 g         2071 t
ORIGIN      
        1 cttaagttta atacctcaat gtgtatcaca atatccacat tctttgtagg ggagtctgga
       61 gattgagtag atattcttgt tcagaatgta tcagccgatg gttctacgat tcttaagcca
      121 cgaagagttc agaaagtgta acggcatgtc tgcaaaagcc agcatctttt gactatctgg
      181 caaccggtag tgtgttctct cacgcatcat aaaagcagca ggcataaaaa aaaacccgct
      241 tgcgcgggct ttttcacaaa gcttcagcaa attggcgatt aagccagttt gttgatctgt
      301 gcagtcaggt tagccttatg acgtgcagct ttgtttttgt ggatcagacc tttagcagcc
      361 tgacggtcca cgatcggttg catttcgtta aatgctttct gtgcagcagc tttgtcgcca
      421 gcttcgatag ctgcgtatac tttcttgatg aaagtacgca tcatagagcg acggcttgcg
      481 ttgtgcttac gagccttttc agactgaatg gcgcgcttct tagctgattt gatattagcc
      541 aaggtccaac tcccaaatgt gttctatatg gacaattcaa aggccgagga atatgccctt
      601 ttagccttct tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc cggcactcgt
      661 tacgtagtga tggcgcagga ttctaccagc ttgcggggtg tgaatacagc ttttccgcga
      721 taaaaattgc agcaggcggt cactttcttc ccgtgatttg cgccatggca atgaaaagcc
      781 acttctttct gatttcggta ctcaatcgcc ggttaacctt gaccgctgta caaggtatac
      841 tcggacgatt ttcactgttt tgagccagac atgaagctga tacgcggcat acataatctc
      901 agccaggccc cgcaagaagg gtgtgtgctg actattggta atttcgacgg cgtgcatcgc
      961 ggtcatcgcg cgctgttaca gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg
     1021 atggtgatgc tttttgaacc tcaaccactg gaactgtttg ctaccgataa agccccggca
     1081 agactgaccc ggctgcggga aaaactgcgt taccttgcag agtgtggcgt tgattacgtg
     1141 ctgtgcgtgc gtttcgacag gcgtttcgcg gcgttaaccg cgcaaaattt catcagcgat
     1201 cttctggtga agcatttgcg cgtaaaattt cttgccgtag gggatgattt ccgctttggc
     1261 gctggtcgtg aaggcgattt cttgttatta cagaaagctg gcatggaata cggcttcgat
     1321 atcactagta cgcaaacttt ttgcgaaggt ggcgtgcgta ttagcagcac cgctgtgcgt
     1381 caggcgctgg cggatgacaa tctggctctg gcagaaagtt tactggggca cccgtttgcc
     1441 atctccgggc gtgtagtcca cggtgatgaa ttagggcgca ctataggttt cccgacggcg
     1501 aatgtaccgc tacgccgtca ggtttccccg gtgaaagggg tttatgcggt agaagtgctg
     1561 ggcctcggtg aaaagccgtt acccggcgtg gcaaacatcg gaacacgccc aacggttgcc
     1621 ggtattcgcc agcagctgga agtgcatttg ttagatgttg caatggacct ttacggtcgc
     1681 catatacaag tagtgctgcg taaaaaaata cgcaatgagc agcgatttgc gtcgctggac
     1741 gaactgaaag cgcagattgc gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca
     1801 aaaccggctt aagcctgtta tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt
     1861 gactataaat caaccctgaa tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc
     1921 aagcgcgaac cgggaatgct ggcgcgttgg actgatgatg atctgtacgg catcatccgt
     1981 gcggctaaaa aaggcaaaaa aaccttcatt ctgcatgatg gccctcctta tgcgaatggc
     2041 agcattcata ttggtcactc ggttaacaag attctgaaag acattatcgt gaagtccaaa
     2101 gggctttccg gttatgactc gccgtatgtg cctggctggg actgccacgg tctgccgatc
     2161 gagctgaaag tagagcaaga atacggtaag ccgggtgaga aattcaccgc cgccgagttc
     2221 cgcgccaagt gccgcgaata cgcggcgacc caggttgacg gtcaacgcaa agactttatc
     2281 cgtctgggcg tgctgggcga ctggtcgcac ccgtacctga ccatggactt caaaactgaa
     2341 gccaacatca tccgcgcgct gggcaaaatc atcggcaacg gtcacctgca caaaggcgcg
     2401 aagccagttc actggtgcgt tgactgccgt tctgcgctgg cggaagcgga agttgagtat
     2461 tacgacaaaa cttctccgtc catcgacgtt gcttttcagg cggtcgatca ggatgcactg
     2521 aaagcaaaat ttgccgtaag caacgttaac ggcccaatct cgctggtgat ctggaccact
     2581 acgccgtgga ctctgcctgc gaaccgcgca atctctattg cacctgattt cgactatgcg
     2641 ctggtgcaga tcgacggtca ggccgtgatt ctggcgaaag atctggttga aagcgtaatg
     2701 cagcgtatcg gcgtgaccga ttacaccatt ctcggcacgg taaaaggtgc ggagcttgag
     2761 ttgctgcgct ttacccatcc gtttatgggc ttcgacgttc cggcaatcct cggcgatcac
     2821 gttaccctag atgccggtac cggtgccgtt cacaccgcgc ctggccacgg cccggacgac
     2881 tatgtgatcg gtcagaaata cggcttggaa accgctaacc cggttggccc ggacggcact
     2941 tatctgccgg gcacttatcc gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc
     3001 gtcgttgcgc tgctgcagga aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc
     3061 tatccgtgct gctggcgtca caaaacgccg atcatcttcc gcgcgacgcc gcagtggttc
     3121 gtcagcatgg atcagaaagg tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag
     3181 tggatcccgg actggggcca ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg
     3241 tgtatctccc gtcagcgcac ctggggcgta ccgatgtcac tgttcgtgca caaagacacg
     3301 gaagagctgc atccgcgtac tctcgaacta atggaagaag tggcaaaacg cgttgaagtt
     3361 gacggcatcc aggcgtggtg ggatcttgat gcgaaagaga tcctcggcga tgaagctgat
     3421 cagtacgtga aagtgccgga cacattggat gtatggtttg actccggatc tacccactct
     3481 tctgttgttg acgtgcgtcc ggaatttgcc ggtcacgcag cggacatgta tctggaaggt
     3541 tctgaccagc accgtggttg gttcatgtct tccctaatga tctccaccgc gatgaaaggc
     3601 aaagcgccgt atcgtcaggt actgacccac ggctttaccg tggatggtca gggtcgcaag
     3661 atgtctaaat ccatcggcaa taccgtttcg ccgcaggatg tgatgaacaa actgggcgcg
     3721 gatattctgc gtctgtgggt ggcatcaacc gactacaccg gtgaaatggc cgtttctgac
     3781 gagatcctga aacgtgctgc cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg
     3841 ctggcaaacc tgaacggttt tgatccagca aaagatatgg tgaaaccgga agagatggtg
     3901 gtactggatc gctgggccgt aggttgtgcg aaagcggcac aggaagacat cctcaaggcg
     3961 tacgaagcat acgatttcca cgaagtggta cagcgtctga tgcgcttctg ctccgttgag
     4021 atgggttcct tctacctcga catcatcaaa gaccgtcagt acaccgccaa agcggacagt
     4081 gtggcgcgtc gtagctgcca gactgcgctg tatcacatcg cagaagcgct ggtgcgctgg
     4141 atggcaccaa tcctctcctt caccgctgat gaagtgtggg gctacctgcc gggcgaacgt
     4201 gaaaaatacg tcttcaccgg tgagtggtac gaaggcctgt ttggcctggc agacagtgaa
     4261 gcgatgaacg atgcgttctg ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc
     4321 attgagcaag cgcgtgccga caagaaagtg ggtggctcgc tggaagcggc agtaaccttg
     4381 tatgcagaac cggaactgtc ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc
     4441 ctgttgacct ccggcgctac cgttgcagac tataacgacg cacctgctga tgctcagcag
     4501 agcgaagtac tcaaagggct gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca
     4561 cgctgctggc actacaccca ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc
     4621 cgctgtgtca gcaacgtcgc cggtgacggt gaaaaacgta agtttgcctg atgagtcaat
     4681 cgatctgttc aacagggcta cgctggctgt ggctggtggt agtcgtgctg attatcgatc
     4741 tgggcagcaa atacctgatc ctccagaact ttgctctggg ggatacggtc ccgctgttcc
     4801 cgtcgcttaa tctgcattat gcgcgtaact atggcgcggc gtttagtttc cttgccgata
     4861 gcggcggctg gcagcgttgg ttctttgccg gtattgcgat tggtattagc gtgatcctgg
     4921 cagtgatgat gtatcgctcg aaggccacgc agaagctaaa caatatcgct tacgcgctga
     4981 ttattggcgg cgcgctgggc aacctgttcg accgcctgtg gcacggcttc gttgtcgata
     5041 tgatcgactt ctacgtcggc gactggcact tcgccacctt caaccttgcc gatactgcca
     5101 tctgtgtcgg tgcggcactg attgtgctgg aaggtttttt gccttctaga gcgaaaaaac
     5161 aataataaac cctgccggat gcgatgctga cgcatcttat ccggcctaca gattgctgcg
     5221 aaatcgtagg ccggataagg cgtttacgcc gcatccggca aaaatcctca aatataagag
     5281 caaacctgca tgtctgaatc tgtacagagc aatagcgccg tcctggtgca cttcacgcta
     5341 aaactcgacg atggcaccac cgccgagtct acccgcaaca acggtaaacc ggcgctgttc
     5401 cgcctgggtg atgcttctct ttctgaaggg ctggagcaac acctgttggg gctgaaagtg
     5461 ggcgataaaa ccaccttctc gttggagcca gatgcggcgt ttggcgtgcc gtcaccggac
     5521 ctgattcagt acttctcccg ccgtgaattt atggatgcag gcgagccaga aattggcgca
     5581 atcatgcttt ttaccgcaat ggatggcagt gagatgcctg gcgtgatccg cgaaattaac
     5641 ggcgactcca ttaccgttga tttcaaccat ccgctggccg ggcagaccgt tcattttgat
     5701 attgaagtgc tggaaatcga tccggcattg gaggcgtaac atgcagatcc tgttggccaa
     5761 cccgcgtggt ttttgtgccg gggtagaccg cgctatcagc attgttgaaa acgcgctggc
     5821 catttacggc gcaccgatat atgtccgtca cgaagtggta cataaccgct atgtggtcga
     5881 tagcttgcgt gagcgtgggg ctatctttat tgagcagatt agcgaagtac cggacggcgc
     5941 gatcctgatt ttctccgcac acggtgtttc tcaggcggta cgtaacgaag caaaaagccg
     6001 cgatttgacg gtgtttgatg ccacctgtcc gctggtgacc aaagtgcata tggaagtcgc
     6061 ccgcgccagc cgtcgtggcg aagagtcgat tctcatcggc cacgccgggc atccggaagt
     6121 ggaaggtaca atgggccagt acagtaaccc ggaaggggga atgtatctgg tcgaatcgcc
     6181 ggacgatgtg tggaaactga cggtcaaaaa cgaagagaag ctctccttta tgacccagac
     6241 cacgctgtcg gtggatgaca cgtctgatgt gatcgacgcg ctgcgtaaac gcttcccgaa
     6301 aattgtcggt ccgcgcaaag atgacatctg ctacgccacg actaaccgtc aggaagcggt
     6361 acgcgccctg gcagaacagg cggaagttgt gttggtggtc ggttcgaaaa actcctccaa
     6421 ctccaaccgt ctggcggagc tggcccagcg tatgggcaaa cgcgcgtttt tgattgacga
     6481 tgcgaaagat atccaggaag agtgggtgaa agaggttaaa tgcgtcggcg tgactgcggg
     6541 cgcatcggct ccggatattc tggtgcaaaa tgtggtggca cgtttgcagc agctgggcgg
     6601 tggtgaagcc attccgctgg aaggccgtga agaaaacatt gttttcgaag tgccgaaaga
     6661 gctgcgtgtc gatattcgtg aagtcgatta agtcattagc agcctaagtt atgcgaaaat
     6721 gccggtcttg ttaccggcat tttttatgga gaaaacatgc gtttacctat cttcctcgat
     6781 actgaccccg gcattgacga tgccgtcgcc attgccgccg cgatttttgc ccccgaactc
     6841 gacctgcaac tgatgaccac cgtcgcgggt aatgtctcgg ttgagaaaac cacccgcaac
     6901 gccctgcaac tgctgcattt ctggaatgcg gagattccgc tcgcccaggg agccgctgtg
     6961 ccgctggtac gcgcaccgcg tgatgcagca tctgtgcacg gcgaatcggg aatggctggc
     7021 tacgactttg ttgagcacaa ccgaaagcca atcgggatac cagcgtttct ggcgattcgt
     7081 gatgccctga tgcgtgcacc agagcctgtt accctggtgg ccattggccc gttaaccaat
     7141 attgcgctgt tactttcaca atgcccggaa tgtaaaccgt atattcgtcg tctggtgatc
     7201 atgggcggtt cttctggtcg cggcaactgt acaccaaatg ccgagtttaa tatcgctgcc
     7261 gatccagaag cggctgcctg tgtcttccgt agcggaatcg aaatcgtcat gtgcggtctg
     7321 gatgtcacca atcaggcaat attaacccct gactatctcg ctactttgcc ggagttgaat
     7381 cgcaccggaa aaatgcttca tgcactgttt agccactacc gtagcggcag tatgcaaagc
     7441 ggtctgcgaa tgcacgatct ctgcgccatc gcctggctgg tgcgcccgga cttattcacc
     7501 ctcaagccgt gttttgtcgc ggtagaaact caaggcgaat ttacctcagg cacgacggtg
     7561 gttgatatcg acggttgcct gggcaaacca gccaatgtaa aggtggcatt ggatctggat
     7621 gtgaaaggct tccagcagtg ggtggctgag gtgctggctc tggcgtcgta acctgtcaca
     7681 tgttatcggc atgcagtcat tcatcgactc atgcctttca ctgatatccc tccctgttta
     7741 tcattaattt ctaattatca gcgtttttgg ctggcggcgt agcgatgcgc tggttactct
     7801 gaaaacggtc tatgcaaatt aacaaaagag aatagctatg catgatgcaa acatccgcgt
     7861 tgccatcgcg ggagccgggg ggcgtatggg ccgccagttg attcaggcgg cgctggcatt
     7921 agaaggcgtg cagctgggcg cggcgctgga gcgcgaagga tcttctttac tgggcagcga
     7981 cgccggtgag ctggctggtg cagggaaaac aggcgttacc gtgcaaagca gcctcgatgc
     8041 ggtaaaagat gattttgatg tgtttatcga ttttacccgt ccggaaggta cgctgaacca
     8101 tctcgctttt tgtcgccagc atggcaaagg gatggtgatc ggcactacgg ggtttgacga
     8161 agccggtaaa caggcgattc gtgacgccgc cgccgatatt gcgattgtct ttgccgccaa
     8221 ttttagcgtt ggcgttaacg tcatgcttaa gctgctggag aaagcagcca aagtgatggg
     8281 tgactacacc gatatcgaaa ttattgaagc acatcataga cataaagttg atgcgccgtc
     8341 aggcaccgca ctggcaatgg gagaggcgat cgcccacgcc cttgataaag atctgaaaga
     8401 ttgcgcggtc tacagtcgtg aaggccacac cggtgaacgt gtgcctggca ccattggttt
     8461 tgccaccgtg cgtgcaggtg acatcgttgg tgaacatacc gcgatgtttg ccgatattgg
     8521 cgagcgtctg gagatcaccc ataaggcgtc cagccgtatg acatttgcta acggcgcggt
     8581 aagatcggct ttgtggttga gtggtaagga aaatggtctt tttgatatgc gagatgtact
     8641 taatctcaat aatttgtaac cacaaaatat ttgttatggt
//
LOCUS       DAZPEP010000160         8639 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZPEP010000160 DAZPEP010000000
VERSION     DAZPEP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.161"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(218..1882)
                     /gene="glnS"
                     /locus_tag="ACHUT9_003604"
     CDS             complement(218..1882)
                     /gene="glnS"
                     /locus_tag="ACHUT9_003604"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFR1349992.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2141..4087)
                     /gene="nagE"
                     /locus_tag="ACHUT9_003605"
     CDS             complement(2141..4087)
                     /gene="nagE"
                     /locus_tag="ACHUT9_003605"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFR1349993.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4420..5220
                     /gene="nagB"
                     /locus_tag="ACHUT9_003606"
     CDS             4420..5220
                     /gene="nagB"
                     /locus_tag="ACHUT9_003606"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1349994.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5280..6428
                     /gene="nagA"
                     /locus_tag="ACHUT9_003607"
     CDS             5280..6428
                     /gene="nagA"
                     /locus_tag="ACHUT9_003607"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFR1349995.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6437..7657
                     /gene="nagC"
                     /locus_tag="ACHUT9_003608"
                     /gene_synonym="nagR"
     CDS             6437..7657
                     /gene="nagC"
                     /locus_tag="ACHUT9_003608"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFR1349996.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7705..8457
                     /gene="nagD"
                     /locus_tag="ACHUT9_003609"
     CDS             7705..8457
                     /gene="nagD"
                     /locus_tag="ACHUT9_003609"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFR1349997.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            complement(8508..8639)
                     /locus_tag="ACHUT9_003610"
                     /pseudo
     CDS             complement(8508..8639)
                     /locus_tag="ACHUT9_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2106 a         2278 c         2204 g         2051 t
ORIGIN      
        1 gccgctgaac gcggcgtttt ggattgtttg catatttctt gccggatgcg gcgtgaacgc
       61 cttaaccggc ctacaaaagc acgcaaactc aatatattgc agagatcatg taggcctgat
      121 aagcgtagtg catcaggcaa tttagcgttt gaaactgttt gtctgttcct tgccggatgc
      181 gacgtaaacg ccccatccgg catagcgaaa cttaaaatta ctcgcctact ttcgcccagg
      241 tatcacgcag cccaacggtg cggttaaata ccggtttttc cgccgtagaa tggcggctat
      301 cgaggcagaa gtaaccttca cgctcaaact ggaatgcttt acccgcaacc gcatctttca
      361 gcgacggttc agcaaagccc tgtttgatca ccagcgattc cgggttaatc accgacagga
      421 aatcatccgc agcaccaggg ttcggcacgc tgaacagacg gtcgtacaga cggatttcaa
      481 ccggcagcgc atgtgccgcg ctcacccagt gaataacacc tttgacttta cgaccatctg
      541 ccggatcttt gcttaaggtg tcggcgtcat aagtacagaa gatggtggtg atattgcctt
      601 cggcatcttt ctccacgcgt tctgccttaa tgacgtaagc attacgcaga cgcacttctt
      661 tacccagcac cagacgtttg tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga
      721 tccaaatctc accgctaaac ggcacctggc ggctacccat ttccggtttg ttcggatggt
      781 tcggcatggt aaccatttcg ccttcgccct gatagttttc gataaccagt ttcaccggat
      841 cgataaccgc cattgcgcgc ggggcgtttt cgttgagatc ttcacggatg caggattcca
      901 gcgacgccat ctcaatggtg ttgtcctgct tggtcacgcc gatgcgtttg cagaactcac
      961 gaatagaagc agcagtgtaa ccacgacgac gcagaccgga aatggtcggc atacgcgggt
     1021 catcccagcc ttcaacgtgc ttgtcggtca ccagcaggtt cagcttacgc ttggacatca
     1081 cagtgtattc cagattcagg cgcgagaatt cgtactggcg cgggtgaaca ggaatggtga
     1141 tgttgtccag cacccagtcg tacaggcgac ggttgtcctg gaactcaagc gtacacagag
     1201 agtgcgtaat accttccagg gcatcgctga tgcagtgggt gaagtcgtac atcgggtaga
     1261 tgcaccactt gttgccagtc tggtggtgtt cagcaaattt aatgcggtac agcaccggat
     1321 cgcgcatcac gataaacggt gaagccatgt cgattttcgc acgcaggcag gctttacctt
     1381 cttcaaaacc accggcacgc attttttcga acagcgccag gttctcttca acgctgcggt
     1441 cgcggtacgg gctgttttta cccggttgcg tcagggtgcc gcggtattcg cggatctgtt
     1501 ccggcgtcag ttcatcaacg tacgccaggc ctttattgat cagttcgatc gcataggcgt
     1561 ggagctgatc aaaataatcg gaggagtaac ggacgttacc agaccagtga aaacctaacc
     1621 actctacgtc gtttttgatc gactcaacat actcgatatc ttcttttacc gggttagtgt
     1681 cgtcgaaacg caggttgcac tggcctttat agtcctgggc gatcccgaag ttcaggcaga
     1741 tagatttcgc atggccaata tgcagatagc cattcggctc cggcgggaaa cgggtgtgta
     1801 ctgtggtgtg cttaccactg gccagatctt catcgatgat ctgacggata aagttagtcg
     1861 ggcgggcttc tgcctcactc atcgtggatt cctcaaagcg taaacaacgt ataacggcgt
     1921 atgatcttat aagcgggaca ggctgacaac tgttagttag cgaaaatcga aaatctcact
     1981 gagattatgg ggatgattga tgaaaaaaaa agcggcagag aagatctcca ccgcttttgc
     2041 cagtcccgcg tcattgttgg atgcgcgtct tatccaacca ggactgagct tttgtaggcc
     2101 tgataagacg cgtcaagcgt cgcatcaggc ataaagcaga ttactttttg atttcataca
     2161 gcggtgtttg acccgccaca acattgccct gagctttaat gatcaagcca ctgaaatcgt
     2221 cgatattgct gcaaaccacc gggctaatca tcgaacgggc gttagcgttc aggtaatcca
     2281 gatccatttc cagaatcggt tgccctgcgc tcacctgcgc gccctcttcc accagacgtt
     2341 taaagccttt accttccagc gctacggtgt cgatacccat gtggacgaca atctccgcgc
     2401 ctttttcggt ttctaggcag aacgcgtggt tggtgttgaa gattttcacg attgtccctg
     2461 cggctggtga tacgacgatt ttatctgtcg gtttcaccgc cacaccgtca cccaccgctt
     2521 tgctggcgaa tgcttcgtca gaaacctgat ccagtgccac gacatcaccg gtaatcggcg
     2581 ataccagctc cgcgatagat accgcgtttg gtacagcctg cggttttgct acaggagcgg
     2641 cagttgccgg agttgcttca gctgacgcag ctgctaccgg gccacgggca acgactttct
     2701 tcatcgcatc gccaatggat tctgctttcg cgccaacaat cacctgaatg gtctgtttgt
     2761 tcagttttac tacaccagaa gcacccagac gtttacacat cgcatcgttg acgcgagctg
     2821 agtcagccac ggtaaggcgc agacgggtaa tacaggcgtc aatcgctttc aggttgtcag
     2881 taccgccaac cgcagcaata tagttggttg ccagttgagt cagaccttct tcagtattgc
     2941 tgttagcctc ttcagttacg atctcgtctt ctttatcttc acgacccggc gttttcaggt
     3001 tgaacatgcg gataaccaaa ctgaacacca cgaagtagat agcgaagaag acaacaccca
     3061 tcaccagcag catccagacg ttctggctgg cggccggcag gttatacatc aacgcgtagt
     3121 cgatagcccc cgcagagaaa gagaagccag catggatacc cagcagcgtt gccacaaaca
     3181 ggctgatacc ggtcagcagt gcgtgcagga ggtacagcag cggagcaagg aacatgaaca
     3241 ggaattccag cggctcagtc acaccggtca ggaacgcagt aacagcaaca gaaagcagca
     3301 tcccgccaac catcggacga cgctctttcg gtgctgcgaa gtacatcgcc agcgccgcac
     3361 ccggcagacc gaacatcatg atcgggaaga agccggacat gaacatcccc gcggtgccgt
     3421 caccagcgta gaaacggttg atgtcgccgt ggaaaaccgt acccgccgcg ttggtgaatt
     3481 caccaatctg gaaccaggcg atggtgttca gcacctgatg cagaccggtt gggatcagca
     3541 gacggttgat gaaaccaaag ataccggaac ccagcgcgcc tgcagaaacg atccactcgc
     3601 cgcctgcatg gatagcgtgc tgtactggcg gccagacgta accaaaaatg gccgccagca
     3661 ccaggcagaa gaagccggtg gcaatcggca caaagcgttt gccgccgaag aagctcagga
     3721 agtccggcag tttaatatcg gaccaacggt tataggctgc gccaccaacc agaccggtaa
     3781 tgatacccgc cagtacaccc atgttaattt ctgggttgat ggtcaccatc gctttggtta
     3841 acacaaagta acctaccgca cccgccagcg ccgccgcacc tgcgctgtct ttcgaccagc
     3901 tggatgccac accgatggcg aagattaatg cgaggttatc aaaaatcgca ccgcccgcct
     3961 gggcaataaa cgcaacgtta agtaaatctg gctgaccgaa tcgcagcaac agtgccgcca
     4021 ccggcagcac cgcgataggg agctgtaacg ccctaccgag tcgctggaaa aaacctaaaa
     4081 tattcatctt attcccccta cgagaaccct atttggctcg tttaaagccg tatttttatt
     4141 ttgctgcaaa ttgtactgcc gatgttctgt aatcagattg ttagatcatc tgctacagag
     4201 tgtgtgaaaa tttaattcgt atcgcaaatt aaacgcgtgc cttttgtgag ttttgtcacc
     4261 aaatatcgtt atcatcactc ccttttactg gctaaagcag aaaacttatt ttatcattca
     4321 aaaaatcagg tcggattgac gcctgtctgc gcaaatccag gttacgctta aagatgccta
     4381 atccgccaac ggcttacatt ttacttattg aggtgaataa tgagactgat ccccctaact
     4441 accgctgaac aggtcggcaa atgggctgct cgccatatcg tcaatcgtat caatgcgttc
     4501 aaaccgactg ccgatcgtcc gtttgttctg ggcctgccga ctggcggcac gccgatgacc
     4561 acctataaag cgttagtcga aatgcataaa gcaggccagg tcagctttaa gcacgttgtc
     4621 accttcaaca tggacgaata tgtcggtctg ccgaaagagc atccggaaag ctactacagc
     4681 tttatgcacc gtaatttctt cgatcacgtt gatattccag cagaaaacat caaccttctc
     4741 aacggcaacg ccccggatat cgacgccgaa tgccgccagt atgaagaaaa aatccgttct
     4801 tacggaaaaa ttcatctgtt tatgggcggt gtaggtaacg acggtcatat tgcatttaac
     4861 gaaccggcgt cttctctggc ttctcgtact cgtatcaaaa ccctgactca tgacactcgc
     4921 gtcgcgaact ctcgtttctt tgataacgat gtcaatcagg tgccaaaata tgccctgact
     4981 gtcggtgttg gtacattgct ggatgccgaa gaagtgatga ttctggtgct gggtagccag
     5041 aaagcactgg cactgcaggc tgcagttgaa ggttgcgtga accacatgtg gaccatcagc
     5101 tgtctgcaac tgcatccaaa agcgatcatg gtgtgcgatg aaccttccac catggagctg
     5161 aaagttaaga ctttaagata tttcaatgaa ttagaagcag aaaatatcaa aggtctgtaa
     5221 ttgttatccc tgccctggct ccttgctcag ggcaatattt tttaaaatcg gggggcagaa
     5281 tgtatgcatt aacccagggc cggatcttta ccggccacga atttcttgat gaccacgcgg
     5341 ttgttatcgc tgatggcctg attaaaagcg tctgtccggt agcggaactg ccgccagaga
     5401 tcgaacaacg ttcactgaac ggggccattc tctcccccgg ttttatcgat gtgcagttaa
     5461 acggctgcgg cggcgtacag tttaacgaca ccgctgaagc ggtcagcgtg gaaacgctgg
     5521 aaatcatgca gaaagccaat gagaaatcag gctgtactaa ctatctgccg acgcttatca
     5581 ccaccagcga tgagctgatg aaacagggcg tgcgcgttat gcgcgagtac ctggcaagac
     5641 atccgaatca ggcgttaggt ctgcatctgg aaggtccgtg gctgaatctg gtaaaaaaag
     5701 gcacccataa tccgaatttt gtgcgtaagc ctgatgccgc gctggtcgat ttcctgtgtg
     5761 aaaacgccga cgtcattacc aaagtgaccc tggcaccgga aatggttcct gcggaagtca
     5821 tcagcaaact ggcaaatgcc gggattgtgg tttctgccgg tcactccaac gcgacgttga
     5881 aagaagcgaa agccggtttc cgcgcgggga ttacctttgc cacccatctg tacaacgcga
     5941 tgccgtatat taccggtcgt gaaccgggcc tggcgggcgc gatcctcgac gaagctgaca
     6001 tttattgcgg tattatcgct gatggcctgc atgttgatta cgccaacatt cgtaacgcta
     6061 aacgccttaa aggcgacaaa ctgtgtctgg ttaccgacgc caccgcgcca gcaggtgcca
     6121 acattgaaca gttcattttt gcgggtaaaa caatatacta ccgtaacgga ctttgtgtgg
     6181 atgagaacgg tacgttaagc ggttcatcct taaccatgat tgaaggcgtg cgtaatctgg
     6241 tcgaacattg cggtatcgca ctggatgaag tgctgcgtat ggcgacgctc tatccggcgc
     6301 gtgcgattgg cgttgagaaa cgtctcggca cactcgccgc aggtaaagta gccaacctga
     6361 cctcattcac acctgatttt aaaatcacca agaccatcgt taacggtaac gaggtcgtaa
     6421 ctcaataaga gaaagtatga caccaggcgg acaagctcag ataggtaatg ttgatctcgt
     6481 aaaacagctt aacagcgcgg cggtttatcg cctgattgac cagtacgggc caatctcgcg
     6541 gattcagatt gccgagcaaa gccagcttgc ccccgccagc gtaaccaaaa ttacgcgtca
     6601 gcttatcgaa cgcgggctga tcaaagaagt tgatcagcag gcctccaccg ggggccgccg
     6661 cgctatctcc atcgtcaccg aaacccgcaa tttccacgca atcggcgtac ggcttggtcg
     6721 tcacgacgcc accatcaccc tgtttgatct cagcagcaaa gtgctggcag aagaacatta
     6781 cccgctgccg gaacgtaccc agcaaacgct ggaacatgcc ctgttgaatg ccattgctca
     6841 gtttattgat agctaccagc gcaaactgcg cgagctgatc gcgatttcgg tgatcctgcc
     6901 agggcttgtt gacccggaca gcggcaaaat tcattacatg ccgcatattc aggtagaaaa
     6961 ctgggggctg gtagaagctc tggaagaacg ttttaaagtg acctgtttcg ttggtcacga
     7021 tatccgtagt ctggcgctgg cggagcacta cttcggtgca agtcaggatt gcgaagactc
     7081 cattctggtg cgtgtccatc gcggaaccgg ggccgggatt atctctaacg ggcgcatttt
     7141 tattggccgc aacggtaacg tcggtgaaat tggccatatt caggtcgaac cgctgggtga
     7201 acgctgccac tgcggcaact ttggctgcct ggaaactatc gccgccaacg ctgccattga
     7261 acaacgggtg ttgaatctgt taaagcaggg ctaccagagc cgcgtgccgc tggacgactg
     7321 caccatcaaa actatctgca aagccgcgaa caaaggcgat agtctggcgt cggaagtaat
     7381 tgagtatgtc ggtcgtcatc tgggtaaaac catcgccatt gctatcaact tatttaatcc
     7441 gcaaaaaatt gttattgccg gtgaaatcac cgaagccgat aaagtgctgc tccctgctat
     7501 tgaaagctgc attaataccc aggcgctgaa ggcgtttcgt actaatctgc cggtggtacg
     7561 ttctgagctg gatcaccgct cggcaatcgg cgcttttgcg ctggtaaaac gcgccatgct
     7621 caacggtatt ttgctccagc atttgctgga aaattaatgt gcttttatag tggcgcttat
     7681 tgttgtcaat attctgggta gtccatgacc attaaaaatg taatttgcga tatcgacggc
     7741 gtgctgatgc acgataacgt cgccgtaccg ggtgcagcgg aatttttgca cgggattatg
     7801 gataaaggcc tgccgctggt gttgctgacc aactatcctt cgcagaccgg gcaagatctg
     7861 gcgaaccgct ttgccactgc cggtgtcgat gtgccggata gcgtgtttta tacctctgcg
     7921 atggcgaccg ccgatttcct gcgtcgccag gaaggcaaga aagcatatgt ggtgggcgaa
     7981 ggcgcactga ttcatgaact gtacaaagcc ggtttcacta ttaccgatgt gaaccctgat
     8041 ttcgtgattg ttggcgaaac gcgttcctac aactgggaca tgatgcataa agcggcctat
     8101 ttcgtcgcta acggtgcacg ctttatcgcc accaacccgg acacccacgg gcgcggtttt
     8161 tatcccgctt gtggcgcgtt gtgtgcaggg attgagaaaa tctccgggcg caaaccgttc
     8221 tatgttggta agcccagccc gtggatcatc cgcgcagcat taaacaaaat gcaggcgcat
     8281 tcggaagaaa cggtgattgt cggcgataac ttgcgcaccg acattctggc gggcttccag
     8341 gcaggtctgg aaacgattct ggtgctttct ggtgtttcat cgctcgacga tatcgacagt
     8401 atgccgttcc gcccaagctg gatttacccg tcagtcgctg aaatcgacgt tatctgaata
     8461 ccacccacgt ctgcggacgt ggttggctct gatgacaaac gccaaactgc ctgatgcgct
     8521 acgcttatca ggcctacgca gctcctgcaa tatattgaat tttcatgctt ttgtaggccg
     8581 gataaggcgt tcacgccgca tccggcatga acaaatcgca ctttatcagc aatttggcc
//
LOCUS       DAZPEP010000161         8634 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZPEP010000161 DAZPEP010000000
VERSION     DAZPEP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8634
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.162"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUT9_003611"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="ACHUT9_003611"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFR1349998.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUT9_003612"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="ACHUT9_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFR1349999.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUT9_003613"
     CDS             4609..5766
                     /gene="mdoC"
                     /locus_tag="ACHUT9_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFR1350000.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUT9_003614"
     CDS             complement(5774..7255)
                     /gene="clsC"
                     /locus_tag="ACHUT9_003614"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFR1350001.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUT9_003615"
     CDS             complement(7197..7730)
                     /gene="ymdB"
                     /locus_tag="ACHUT9_003615"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFR1350002.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUT9_003616"
     CDS             complement(7825..8136)
                     /gene="ymdA"
                     /locus_tag="ACHUT9_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFR1350003.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUT9_003617"
     CDS             complement(8257..8589)
                     /gene="csgC"
                     /locus_tag="ACHUT9_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFR1350004.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2155 a         2247 c         2051 g         2181 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa tagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aagcacttcc ccctttgtgt
     4261 gcttatatcg atccgatcta ttttaatgga taaccagcca ttccgacaac agaatcccgg
     4321 tatcgttcag cacgctggct catggattaa gcacctgatt gcaaccgttt tcactttccg
     4381 tgcttctcct aaagacagaa gcatcgactt cgtgtagggt ttgccagcaa ttttatttat
     4441 caaccgtctt acagacgctt ccattctggt tagtggtagc gatgatgcta tgcataaaaa
     4501 ccttgaagcc tgcgcctggc gcggtcaggt cggtgtcgct atggcaacaa gcctaaaggc
     4561 ataacccgac agggataaga cgaaaaatcc gagattagtt aaccatatat gaatccagta
     4621 cccgcgcaac gtgaatattt cctcgactcc atccgcgcct ggctgatgtt gttagggatc
     4681 ccttttcata tttctttaat ctattcgagc catacatggc atgtgaatag cgccgaaccg
     4741 tcattgtggc tgaccctttt taatgacttc atccactcgt tccgcatgca ggtatttttc
     4801 gttatatccg gctacttttc ctacatgctt tttttacgct atcccttgaa aaaatggtgg
     4861 aaagtacgtg tcgaacgtgt aggtatcccg atgttaacag ccatcccctt actgacattg
     4921 ccgcaattta ttatgctgca atacgtcaaa gggaaagcgg aaagttggcc tgggctgtca
     4981 ttgtatgaca aatataatac gttggcctgg gaattaatat cacacctgtg gtttttactg
     5041 gtgttagtag tcatgacgac gctgtgcgta tggatattta aacgcatcag aaataattta
     5101 gaaaattctg ataaaacgaa taaaaaattc tcgatggtaa aactatcggt gattttttta
     5161 tgcctcggca tcggctatgc ggtaataaga agaacgattt ttattgtgta tccgcccatt
     5221 ctgagtaatg gcatgttcaa ttttattgtc atgcaaacgc tattttattt gccgttcttt
     5281 atcctcggtg cactggcttt cattttccct catcttaaag ccttgtttac cacgccgtct
     5341 cgtggctgta cccttgcagc agcattggca tttgtcgctt atttactcaa ccagcgctat
     5401 ggcagtggcg atgcctggat gtacgaaacc gagtcggtga tcaccatggt cctcggtctg
     5461 tggatggtga atgtggtctt ctcctttggc caccgtttgc ttaacttcca gtcagcgcgg
     5521 gtgacttact ttgttaacgc atcgctattt atctatctgg ttcaccaccc gttaacgctg
     5581 tttttcggcg catacattac accgcacatc acctccaact ggcttggttt tctctgtggc
     5641 ctgatattcg tagtagggat tgcgataatt ctgtatgaaa ttcatttacg catcccgtta
     5701 ctgaagtttt tgttttctgg taaaccggtt gttaagcgtg agaacgataa agcaccagcc
     5761 cgttaagcca catttacaat aaccattcca cgggcaatat cgacgccagt ctgaccataa
     5821 cccgcttcca gaaactggtg gcgggttctt ttttgagaat aatctctttc tttgcatgac
     5881 gatcaaccca gttgatccgt ccccacctgt ccagacggag ctgccaggcc gcatcatact
     5941 ggctctgaat aaagcgttta tcaattaact gtgccagcgt ttcgctctct atcacgaagc
     6001 ccatttcagt attgagcaat gttgaacgcg gatcgaaatt gaaagagccg ataaacaccg
     6061 ttttaccatc gatgctaaaa gttttagcat gcaggctggc tccggaatta ccggttatgc
     6121 cgcgatcgtg taacgtacta ctttgttcac gcgtcggctt gagttcatat aattccacgc
     6181 catagcggag caattttttg cgccagcgcg cgtatccggc atggacgaca gcaacatcgt
     6241 tagcggcaag agaattggtt aggatcgcaa tctttacccc ttttctcacc atccgtaaga
     6301 gttgcgccac acctgcgcgt gtcggtacaa aataggaaga gataatatcg atgcgttcac
     6361 tgggtgagcc catgatatcg gacaggcgct gcggtaacag tgaatgccgt tttgccttgc
     6421 cctccccttt cgccggatca tcacttaata aacgtgtttt cgcccagata agcggcaacg
     6481 ttccatcaac cagatgattt ataaacggac tggattccat ttggcgtaag taacgatgcg
     6541 tcatggcatc gttatgccag gaggcgggta actcgatgcg atccgccatt tcaccttccg
     6601 ggacatccag cacctgctgt aagggtgaaa ccgatttgca ataccagtag cgggcgaaat
     6661 catcggcaac gtcctctacc acgggtccta ttgccataac atctaaatcc gaaaaaagtg
     6721 gctcttcccc tgccccaaaa taggcatcac caatatttcg tcctcccacc agggtaacca
     6781 cgccatcgac agtgaaactt ttattgtgca tacggcgatt gagacgggaa aagtcggtga
     6841 tataaccaag cggacgcagc aagcgaaacg agaaaggatt aaaaagccgg acttcaatgc
     6901 ggggatggct gtcaagcaag cgtaaaatat cgtcaagtcc gggcgtattg ttgtcgtcca
     6961 gcaacaaacg gacgcgaacg ccacgctttg ctgcggctaa cagggcggaa aacagtaacc
     7021 gacccgacat atcgtcctgc cagatgtaat actgaacatc cagcgtatgc tctgccattt
     7081 cggccagacg ataccgggcg gcaaacgcat ccagactttt ctccagaggg aaaagaccac
     7141 actgaccggg atgttgcgaa cacagtggca gcaccgcgct cgccagccgg ggcaaatcat
     7201 tcatctcctt gttgggtaag gagtctttcg tagaggtggg cgttttcttc atcataacag
     7261 acaaagtata cctgttcggg taaagcgtga cgggtaataa attctgaaac ggttttaact
     7321 gcgatttcag ccgctgccgc acgagggtaa ccataaaccc cagtactgat tgcaggaaaa
     7381 gccactgacg tatagctgtt tgccgccacc agtcgcaggc tattcagata ggcatcctgc
     7441 aaaagctggt cttcgttttg ttcaccacca cgccagactg gcccgacggt gtgtactacg
     7501 gctttagcgg gaagatcgcc tgcaagcgta ataacggcat gccccgtagg gcaatcgccc
     7561 tgctgttgcc tgacttttaa acaggcatcc agcagggccg gacccgctgc gcgatgaatg
     7621 gccccatcga cgccgccgcc tcccattaat gacggattag ccgcattcac aatcacatca
     7681 acggccagtt tggtaatatc accctgcaca acatgaatac gcgttttcat aacgcctcct
     7741 tacaccttct tgtttcttaa gtgtacagca ggctttttgg ttaaatcgca tttttctgct
     7801 cagcaacgtg cgttgttcag aaacttagtt atattcgatg ttcattacag ccagcttttt
     7861 ctgtttatca agatactgca cttttactga ggctatttgc gcgtttttaa cattaccgct
     7921 cattaacgct ttgctggaat aggttttgcc cggaacagaa ccattctgtg ggcagttcat
     7981 ttctacgggt gatgactggg tgctgacgtc acatgccggt tccacaattt ggccataaaa
     8041 atggatcaca cccccttgcg cggtgcttcc tgcgatggca ataaaaggaa aaaacaaact
     8101 gccgagcata agagagttta aaaaaggtct gaacatttat tctatttcct caatgattag
     8161 tcatccttga gggttgtgtt atccatactt tcaggttggc tgattcgcag cagaccattc
     8221 tctccagatt catcttatgc tcgatatttc aacaaattaa gacttttctg aagagggcgg
     8281 ccattgttgt gataaatgaa gtgcctgtcc atcagaaaca gtaacaacta ttttcacccg
     8341 atcttccggg gaaatattta aactcaactt cgtcaaagca ataggttgat tagcaggcaa
     8401 tgagagggtc ttttcttgct tcgtctgact ttgccctgaa ctgccttcgc gcagggacaa
     8461 tatttgtact ctgcacagac aagattgagt aagagtgact tcaggaataa tggtatacac
     8521 atccccttgc tgggtcgtat taaaggttat ctggctggaa agtgccgcaa ggagtaataa
     8581 cgcattcata ttcttctccc gaaaaaaaca gggcttgcgc cctgtttctg taat
//
LOCUS       DAZPEP010000162         8509 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZPEP010000162 DAZPEP010000000
VERSION     DAZPEP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.163"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(160..2151)
                     /gene="tkt"
                     /locus_tag="ACHUT9_003618"
     CDS             complement(160..2151)
                     /gene="tkt"
                     /locus_tag="ACHUT9_003618"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFR1350005.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2429..3187
                     /gene="loiP"
                     /locus_tag="ACHUT9_003619"
                     /gene_synonym="yggG"
     CDS             2429..3187
                     /gene="loiP"
                     /locus_tag="ACHUT9_003619"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFR1350006.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(3330..4250)
                     /gene="speB"
                     /locus_tag="ACHUT9_003620"
     CDS             complement(3330..4250)
                     /gene="speB"
                     /locus_tag="ACHUT9_003620"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFR1350007.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(4388..6364)
                     /gene="speA"
                     /locus_tag="ACHUT9_003621"
     CDS             complement(4388..6364)
                     /gene="speA"
                     /locus_tag="ACHUT9_003621"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFR1350008.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(6373..6504)
                     /gene="yqgB"
                     /locus_tag="ACHUT9_003622"
     CDS             complement(6373..6504)
                     /gene="yqgB"
                     /locus_tag="ACHUT9_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFR1350009.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(6598..6741)
                     /gene="yqgG"
                     /locus_tag="ACHUT9_003623"
     CDS             complement(6598..6741)
                     /gene="yqgG"
                     /locus_tag="ACHUT9_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFR1350010.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(6852..7103)
                     /gene="yqgD"
                     /locus_tag="ACHUT9_003624"
     CDS             complement(6852..7103)
                     /gene="yqgD"
                     /locus_tag="ACHUT9_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFR1350011.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            7159..8313
                     /gene="metK"
                     /locus_tag="ACHUT9_003625"
     CDS             7159..8313
                     /gene="metK"
                     /locus_tag="ACHUT9_003625"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFR1350012.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2070 a         2299 c         2187 g         1953 t
ORIGIN      
        1 atgtcaatac gcatatcgtg atgcatatta cataattgat atttattgca tataaatatt
       61 tgtgatctac aacacgcttt atctattgct tgtccgcaaa cggacatatc aaggtaataa
      121 aaaaggtcgc cgaagcgacc tttttacccg aaatgctaat tacagcagtt cttttgcttt
      181 cgcaacgacg ttgtcgacgg tgaagccgaa ctcttcgaac agcaactctg ccggagctga
      241 ctcaccgaag gtggtcatac cgatgatagc gccattcagg ccaacatact tgtaccagta
      301 gtcagcaata cccgcttcta ccgcaacgcg tgcagtaacc gctttcggca gtacggattc
      361 acggtaagcc gcatcctgct tgtcgaatgc atcggtagac ggcatggaaa ccacgcgcgc
      421 cttcacgcct tcggcagtca gtttttcgta ggcggcaaca gccagttcaa cttccgaacc
      481 ggtcgcgatg aagatcagtt ccggctgacc agcacagtct ttcagcacat aaccaccgcg
      541 cgcgatgttt gccagttgct cttcagtacg ttcctgctgc gccagattct gacgggagag
      601 gataagcgcg gtcggaccgt cctgacgctc aacaccgtat ttccacgcga ccgcggattc
      661 aacctggtca cacggacgcc atgtagacat gttcggggtc acgcgcagag aagcgacctg
      721 ctcaaccggc tggtgagtcg ggccgtcttc gcccagaccg atggagtcgt gggtgtaaac
      781 catcacctga cgctgtttca tcagcgcagc catacgtaca gcgttacgtg cgtattcgac
      841 aaacatcagg aaggtggagg tgtacggcag gaagccaccg tgcagggaga taccgttagc
      901 aatcgcggtc ataccgaact cgcgaacacc gtagtggatg tagttacccg cagcatcttc
      961 gttgattgct ttagaaccag accacagggt caggttagac ggtgccaggt cagcggagcc
     1021 gccgaggaat tccggcaaca gcgggccgaa cgcttcaata gcattctgag acgctttacg
     1081 gctggcgatt ttcgccggat tagcctgcag tttagcgata aactctttcg ctttggcgtc
     1141 gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat tcagccgctt cctgcggata
     1201 agctttcgcg taagccgcaa acttctcatt ccatgcagat tctttcgcct ggcctgcttc
     1261 tttcgcatcc cactgcgcat agatttcaga cgggatttcg aacggtgcgt atttccagcc
     1321 cagctgttcg cgggtcagag cgatttctgc atcacccagc ggcgcaccgt gggagtcgtg
     1381 agtaccggct ttgttcgggg aaccgaaacc gatgatggtt ttgcacatca gcagggacgg
     1441 tttgtcagtc actgcgcgcg cttcttctac tgcgcgtttg atggatgctg cgtcatgacc
     1501 gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa cgcattgcgg tgtcgtcggt
     1561 gaaccagcct tcaacgtgac catcaataga gataccgttg tcatcgtaga acgcaatcag
     1621 tttacccagc ttcagcgtac ccgccagaga gcaaacttcg tgggagatgc cttccatcat
     1681 gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca atgtcgtggc ccggacggtt
     1741 aaactgcgcc gccagcgttt tttctgcaat cgccataccg actgcgttgg caataccctg
     1801 acccagcgga ccggtggtgg tttccacacc agcggtataa cctacttccg ggtggcccgg
     1861 agttttagag tgcagctgac ggaagttttt cagttcttcc atcggcagat cgtaaccggt
     1921 gaggtgcagc aggctgtaga tcagcatgga gccgtggccg ttggacagca cgaagcggtc
     1981 acggtcagcc caggacggat tctgcgggtt gtgtttcagg aaatcacgcc acaggacttc
     2041 ggcaatgtca gccataccca taggggcacc cgggtgaccg gatttggctt tctgtactgc
     2101 gtccatgctc agcgcacgaa tagcattggc aagctcttta cgtgaggaca ttttgactcc
     2161 agatcggatg atgaagggca cgcccttaac gacttgacga cagcgcgttt tgggctacgc
     2221 cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat gtacatggaa catccttttg
     2281 ccgcttcaga aatctctgga tcatgctcgc atgttgcgca atctactcgc ccgtccgctg
     2341 cgcttttcct tatactgaga ctgagcgtcg attcacctgc aaacggcgca tttttagaat
     2401 aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc gccttattgg tagcaatgag
     2461 cgtggcaacg gtactgactg gttgccagaa tatggactcc aacggactgc tctcatcagg
     2521 agcggaagct tttcaggctt acagtttgag tgatgcgcag gtgaaaaccc tgagcgatca
     2581 ggcatgtcag gagatggaca gcaaggcgac gattgcgcca gccaatagcg aatacgctaa
     2641 acgtctgaca actattgcca atgcgctagg caacaatatc aacggtcagc cggtgaatta
     2701 caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg gctaacggct gtatccgcgt
     2761 ctatagcggg ctgatggata tgatgacaga taacgaagtc gaagcggtga tcggtcacga
     2821 gatggggcac gtggcgttag gccatgtgaa gaaagggatg caggtggcgc ttggtacaaa
     2881 tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc ggcagtttat ctcaatcaca
     2941 acttggtgat ctgggcgaga aattagtcaa ttcgcaattc tcccagcgcc aggaagcaga
     3001 agccgatgat tattcttacg atcttctgcg ccaacgcggc atcagcccgg caggtcttgc
     3061 caccagcttt gaaaaactgg cgaaactgga agaaggccgc caaagctcaa tgtttgacga
     3121 ccatccggca tccgccgaac gcgcccagca tattcgcgat cgcatgagcg cggatgggat
     3181 taagtaaagc ctggtggtgt cgaacgtagg tcagataagg cgttcacgcc gcatctgaca
     3241 tcaatggcac atttgttacc ctgtgcgcat tgccggatgc gatgctgact gcatcttatc
     3301 cggcctacgg gtggcatcgc atctggtgct tactcgccct ttttcgccgc ttggatatac
     3361 agcatctcca gcgccagcgt cgccgcagcc agagcggtga tttccgactg atcgtatgcc
     3421 ggagccactt ccactacgtc catcccaaca atgttgagat ctttcaggcc gcgtaccagt
     3481 ttaatagcgc gatcggaggt caggccgcca atcactggcg taccggtgcc tggtgcaaaa
     3541 gcaggatcca ggcagtcgat atcaaaagtc aggtaaaccg gcatatcacc cacaatctgt
     3601 ttcacttggg cgataacgtc atccacgctg cgatcgttca cctggcaggc gtccagcacg
     3661 gtaaagccgt tgtctttatc aaactcggta cgaataccaa tctgcacgga atgattcggg
     3721 tcgatcagac cttctttcgg cgcggtatag aacatagtgc cgtggtcaaa ttcacaaccg
     3781 ttcgcatagg tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc gaaatgcttc
     3841 gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac caccgaaggt gagcatacgc
     3901 ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct tttcgctcat ctcacgggca
     3961 tcgccaaagg catataccag atcgccgcag tccacgacgt tcagacgctc acgcatgtcg
     4021 aaattccacg ggaagcggtt gtgttcccag gccagattcg tcgaaacctg acggatcgct
     4081 gccggaccgt ggcgaccacc cgcacgacca gaagtggcca tatcgaacgg cacgccagta
     4141 atcacccagt ctgcatcgct gtcatacggc tggaagttca tcggcaggcg taaaaaacca
     4201 aaggcattgg aaaccagtga gttatcgtat tgatgaccta aggtgctcat ggatggactc
     4261 ctattacaaa gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg aggaagggtt
     4321 ggatttgtca caataaattg tggcggatta tcaccgctaa attaagcgga ttcaagtaac
     4381 acaggactta ctcatcttca agataagtat aaccgtacaa acctgcctcg aactcttcaa
     4441 ggaactgttg ttgcagttca gcatcaagat cggttttctt cacttgatcg cggaactggg
     4501 ttaacagcgt tttcggatcg agctgtacat attgcagcat gtccgccacg gtatcgcctt
     4561 cgtcagacag ttctacttct acgctaccgt cagggaacac gaacacgtca accgcttcgg
     4621 tatcaccgaa caggttgtgc atattgccaa ggatctcctg atatgcgccg accataaaga
     4681 aaccgagcat cggcggattc tctggatcgt actccggcat tggcattgtc gtggcaatac
     4741 catcaccatc aatatagtgg tcgatagcac cgtcagagtc acaggtaata tccagcagca
     4801 cagcgcgacg ttccggcact tgatccagcc cttccagcgg cagaaccggg aacaactggt
     4861 cgatccccca tgcgtccggc attgactgga acagcgagaa attgacgtac attttgtccg
     4921 ccatacgttc ctgcagttcg tcgattatcg gacgatgcgc acggttttgc ggatccagtt
     4981 gcttctgcac ttcatggcac atgctcagat agagttgttc tgcccatgca cgttcttgca
     5041 ggctaaaggt gccggaagag tagccgatat gaatgtcgtg cagatccatc tggctgtcgt
     5101 gtaaccattc acgcaaagaa cggcgagttc ccggttcgtg catttcctgc caggtttccc
     5161 acatgctttg cagcgcgcgc ggcgcatctt ctgcgggcgc ggtcggcacc gtatattcgt
     5221 tacgttccac gccgatgata ttcgacacca gcacggtatg gtgcgcagtc accgcacggc
     5281 ccgattcggt gattaccgtc ggatgcggca gaccattttc ttcacacgca tcgccaatcg
     5341 cccagatgat gttgttggcg tattcattga ggccatagtt tacggagcag tcagactgcg
     5401 aacgggtgcc ttcatagtcc acgcccagac cgccgccgac gtcgaagcac tgaatattga
     5461 cgcccagctt gtgcagttcc acatagaaac gcgcggattc acgaacgccc gtggcgatat
     5521 cgcgaatatt cgccatctgc gaaccgaggt ggaagtgcag tagttgcagg ctgtcgagac
     5581 gcccggcttc acgcagggtt tcaaccagtt gcagtacctg agtcgcagcc aggccgaact
     5641 tcgatttttc cccgccggag gactgccatt tacccgagcc ctgcgaagcc agacgtgcac
     5701 gcacgcccag acgaggaacg acattcagac gttctgcttc atccagcaca atggcgattt
     5761 ctgacatctt ctcaatgacc agatagacct tgtgccccat cttctcgcca attaatgcca
     5821 ggcggatata ttcgcggtct ttataaccgt tgcagacgat gacgctacgg gtcatgccag
     5881 catgtgccag tactgccatc aactcggctt tggaaccggc ttccagaccc agcggttcgc
     5941 ccgaatgaat cagggactca atcacgcggc ggtgctggtt aactttgatc ggataaacaa
     6001 ggaagtaatc gccgttatag ccgtaggatt ccctcgcacg tttgaacgcg gcgttaatgg
     6061 aacgcaaacg gtgctgcagg atctgtggga aacagaacag tgcaggcaga cgctgaccct
     6121 gtgcttcacg agttttcact aactgcgcga gatcgacgcg agcttccggg acgtccgggt
     6181 ccgggcacac gctgatgtgg cccagctcgt taacgtcata gtagttattg ccccaccagg
     6241 caatattgta agtacgcagc atcttgctgg cttcctggga gctcattgca acctcctgca
     6301 tggagcgtag tacaccgtgt tcgcccgctg acgaaggcaa acccatagac atgtcgtcag
     6361 acatagcgaa cctcaaatta ttttattaag tgtaaaacag ttaacgacta tcgcagcctg
     6421 gaactgcgat aacaacccat aagcacatgg attttccagc agtgaatgct gacgctccaa
     6481 ctgcgcaacc ggtttctttt tcataacatt attaagcaca taaccgaacg taagtgtgac
     6541 agttcggcga aaccacgaga aaactcttgt ttttacaaga gcgcccttgt tcagtcctca
     6601 gtaactgtaa ccagctcttg aatcctgaga agcgccgaga tgggtataac atcggcaggt
     6661 atgcaaagca gagatgcaga gtgcggggaa cgaatcttca ccagaacggt gagacaggtt
     6721 aagcagcaga caacggttca ttatttcgta tcacctccac ggccgcctgt taagacgaac
     6781 ccacaagcca aaactctgat ttcaacccgg ctggaagtgg caacacgaaa gaaacgtcgt
     6841 gtgcttttta tttaagccgc gcgccgcgtt ttatacccca caatggcaga aaattgcaaa
     6901 agataaatac gcagaatgcc ggcattgtca ggaaaaattt ccagccacgt ttttaacaga
     6961 atgagacacg attcaaaaaa aagtggaaat cgggtgaaga attgacctaa aatagccatc
     7021 cagatgttaa tccatccata ccgattaaca ctcagactgc cagtgttttt aacctgcaga
     7081 gtcgtggtag gatccgctac cacagaaaat ccacacaaca gtttgagcta accaaattct
     7141 ctttaggtga tattaaatat ggcaaaacac ctttttacgt ccgagtccgt ctctgaaggg
     7201 catcctgaca aaattgctga ccaaatctct gatgccgttt tagacgcgat cctcgaacag
     7261 gatccgaaag cacgcgttgc ttgcgaaacc tacgtaaaaa ccggcatggt tttagttggc
     7321 ggcgaaatca ccaccagcgc ctgggtagac atcgaagaga tcacccgtaa caccgttcgc
     7381 gaaattggct atgtgcattc cgacatgggc tttgacgcta actcctgtgc ggttctgagc
     7441 gctatcggca aacagtctcc tgacatcaac cagggcgttg accgtgccga tccgctggaa
     7501 cagggcgcgg gtgaccaggg tctgatgttt ggctacgcaa ctaatgaaac cgacgtgctg
     7561 atgccagcac ctatcaccta tgcacaccgt ctggtacagc gtcaggctga agtgcgtaaa
     7621 aacggcactc tgccgtggct gcgcccggac gcgaaaagcc aggtgacttt ccagtatgac
     7681 gacggcaaaa tcgttggtat cgatgctgtc gtgctttcca ctcagcactc tgaagagatc
     7741 gaccagaaat cgctgcaaga agcggtaatg gaagagatca tcaagccgat tctgcccgct
     7801 gaatggctga cttctgccac caaattcttc atcaacccga ccggtcgttt tgttatcggt
     7861 ggcccgatgg gtgactgcgg tctgactggt cgtaaaatta tcgttgatac ctacggcggc
     7921 atggcacgtc acggtggcgg agcattctct ggtaaagatc catcaaaagt ggaccgttcc
     7981 gcagcctacg cagcacgtta tgtcgcgaaa aacatcgttg ctgctggcct ggccgatcgt
     8041 tgtgaaattc aggtttccta cgcaatcggc gtggctgaac cgacctccat tatggtagaa
     8101 actttcggta ctgagaaagt gccttctgaa caactgactc tgctggtacg tgagttcttc
     8161 gacctgcgcc catacggtct gattcagatg ctggatctgc tgcacccgat ctacaaagaa
     8221 accgcagcat acggtcactt tggtcgtgaa catttcccgt gggaaaaaac cgacaaagcg
     8281 cagctgctgc gcgatgctgc cggtctgaag taatctttct tcacctgctt tcaaaggcca
     8341 gcctcgcgct ggcctttttc ttttacctca ccgcggtgga taggcattca cgccgcatcc
     8401 ggcaaaaagc tgcccgcaca ataacatcat tcttcctcaa cacgttttat cgcaaattat
     8461 catcactact gaaaccgatt acaccaataa caccagccaa atatttgta
//
LOCUS       DAZPEP010000163         8412 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZPEP010000163 DAZPEP010000000
VERSION     DAZPEP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.164"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..445
                     /locus_tag="ACHUT9_003626"
     CDS             <1..445
                     /locus_tag="ACHUT9_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1350013.1"
                     /translation="LGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSCRTITHY
                     RHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFELLNQLV
                     ESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            491..1744
                     /locus_tag="ACHUT9_003627"
     CDS             491..1744
                     /locus_tag="ACHUT9_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HFR1350014.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLITPKKTITNNPTLLIFISLCLLGIINIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPYIDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(1814..2785)
                     /gene="rfaC"
                     /locus_tag="ACHUT9_003628"
     CDS             complement(1814..2785)
                     /gene="rfaC"
                     /locus_tag="ACHUT9_003628"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFR1350015.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(2789..3835)
                     /gene="rfaF"
                     /locus_tag="ACHUT9_003629"
     CDS             complement(2789..3835)
                     /gene="rfaF"
                     /locus_tag="ACHUT9_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFR1350016.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(3845..4777)
                     /gene="rfaD"
                     /locus_tag="ACHUT9_003630"
     CDS             complement(3845..4777)
                     /gene="rfaD"
                     /locus_tag="ACHUT9_003630"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFR1350017.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4991..6187
                     /gene="kbl"
                     /locus_tag="ACHUT9_003631"
     CDS             4991..6187
                     /gene="kbl"
                     /locus_tag="ACHUT9_003631"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFR1350018.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            6197..7222
                     /gene="tdh"
                     /locus_tag="ACHUT9_003632"
     CDS             6197..7222
                     /gene="tdh"
                     /locus_tag="ACHUT9_003632"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFR1350019.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            7461..8363
                     /locus_tag="ACHUT9_003633"
     CDS             7461..8363
                     /locus_tag="ACHUT9_003633"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFR1350020.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMW"
BASE COUNT         2164 a         1933 c         2008 g         2307 t
ORIGIN      
        1 actaggagaa gatctggatt ttatctatcg actgctaatt acatgcgata tgtatgcggt
       61 tccatattat atgtataagc ataactatag agaaaattcc ttaatgaact catgtagaac
      121 catcactcat tatcgacatg agtcatttgc gcacgaaaga atctactctt ctgtgatgca
      181 gttatacaaa ggtaacagga aagaagaaat tcatacatta ttgagtcaaa atagagctta
      241 tcataaaact cgttatttat ggaatgttct acttaatggt gattttgaac tattaaatca
      301 attagttgaa agcaatgaaa aagaattgaa tgattgtaat ctttctggca agagagataa
      361 aagacgagca aaaatattag catcaaaaaa ttatattctc tggaggatgg taagactggt
      421 aaataggaaa aagaataaac gttagatctt gcattaaata atccgtcctc gtagcatagg
      481 ttgaaactat atgtcatttt gttggaatga aattaattct ggtataaagt ctttaattct
      541 catattatgt attttttctt tgatgacttt gtctttatgg gatgatgttg caacaaagtt
      601 tcttcatgca gctggaatta tatctgcatt gtattttcta ataacaccaa aaaaaacaat
      661 aactaacaat cctactttgt taattttcat ctcattatgt cttttgggaa tcataaatat
      721 catctggtat tcacattata aagtttcagg ctctgtttat accaatgcat atcgtggccc
      781 aatggaaact gggaaaattg ccttgtgtag tgcttttatt ttcttggttc tttttgctaa
      841 aaatgagttg agaacaaaaa taaaatttgg gaaactaatt ctgttcgcat ccctggcaac
      901 gcagttactt ttttttgcgc atgccatgtg gcaacatttc tatttaaacg ttgaccgtgt
      961 tgcattatca gcttcccacg ctacaacagc aggctacatc atccttttcc cttctttact
     1021 ggcatcaatt ctcattttaa aatccgactt tagacataaa acaacattat atacaattaa
     1081 cttcatgctt agcttatgtg ctgtcatagt aactgaaacg cgtgcagcca tattagtgtt
     1141 tccattcttt gcgttactat taatcgtaat ggatagttat attaataagc gaattaatta
     1201 taagttatat tgttttattg cgattgcatt attagcaggt gtattttctt ttaaagatac
     1261 attgcttatg agaatgaatg acttaaataa cgatttagtt aattattcgc atgataacac
     1321 cagaacttca gtcggtgccc gtctggcaat gtatgaagtt ggcttaaaaa catattctcc
     1381 aataggacaa tcactggaaa aacgagcgga aaaaatacat gaactagaag aaaaagagcc
     1441 tagattgagt ggcgctttac cctatataga ttctcatttg cataacgatc tcatagatac
     1501 gttatcaacg cgtggtattc ctggagttgt attaacaatt ttagcctttt cagcaatact
     1561 catatatgcc ttaagaactg ctaaagaacc ttatatttta atcttgcttt tttcactact
     1621 ggtagtaggc ctaagtgatg taatactctt ttctaaaccg gttccgactg ctgtgtttgt
     1681 caccataata ttgctttgtg cttattttaa agcacaatca gaccaatgtt tattagataa
     1741 ataatgaata tgtgaaataa aatcagcaag gaggctgatt ttatttcaaa attatctatt
     1801 aaggggttgt aatttaaatc atagcagcgt tttcttcaat gaatcgctta acagcatttg
     1861 ctgtcaattg agacaactca ttccccggag ccctacaaac catctgattc ttcccatatc
     1921 caccaattaa tcccggatcg gttggtccat aaaccgtgat attgggtcta tccagtgccg
     1981 ccgttaaatg gcttaacccc gtatccaccg acactacaaa tttagccccg gccagcacgc
     2041 gggcaacgcc ttccagactc atcttcggca atacttcaac ataagcaaat ccttccgcca
     2101 gtcgtttcgc ccgctcttcc tcatgcggcg cgccccacgg aagtttaatc cgtattcctg
     2161 aatcagccag taaaccaatc aattctcgcc agtgttcttc cggccagtgt ttatcatcac
     2221 gggtcgtcgc atgaagaaat acggcatatt cgccagcatc tgtaggcagg ttcgtcagga
     2281 aatgctgtgc aatagcataa tcgccctggg tttgcggttt gctatagccc agacttttgg
     2341 caaacagttc gcgggtgcgt tctacggcgt gctgctgttt tgcaatatga tgcttacgat
     2401 tgtaaaacag actggctaaa ggttcgcgag cggtttgcca gtccatgcca tgctttacgc
     2461 catgcgccag acgcgtcacc agcgccgcgc tttttaccag cccctgagcg tcgataactg
     2521 cgtcatagtt ctctgcttgt agcgcttcac gaaacgcttt gcgttccgct tttatggggg
     2581 ccgagaacca ggctttacgc cagcgacgta ttgccacagg aataactcgc tcaacggcgg
     2641 cgtgccagga aggaatctgt gcgaaccctt cttccaccac ccagtcaaac ttaatccctg
     2701 ggattgcctg ctgggcatca gtgagtgcgg gcaacgtatg gagaacatcg cccatcgacg
     2761 atgttttaac gatcaaaacc cgcatccgtc aggcttcctc ttgtaacaat agcgcgttga
     2821 gttcttccag tacgcgctgg ggagtaatgt cgattaagct ctggtgataa ccctccgcag
     2881 cgtcaccttt acgcactttg tgatagccgg taatcaggcg gatcacgcgc gctttatggg
     2941 atagcggcgg tgtgaagtcc gggctactcg gaccatacag ggcaaccagc ggacgattga
     3001 gcgccgccgc aacgtgcatc aggccagaat cgttagtgac aatggcttta caggctgcaa
     3061 tcaggataac cgcttgatca agctgtgttt cccccgccag gttccgacac catgcctgct
     3121 gctcggtatt caaagcggca agaatctcat tgcccgcttc atgatctttc gccgagccaa
     3181 acagaaccac ctgataacct tcatcaatca gctgctttgc cagttccgca tagtggtagt
     3241 gtggccagcg ttttgccgga ccaaactccg cacccgggca aaaaccaatc atcggacgtt
     3301 ctgatgaaag cgaaaattga ttacaggtat atgatttttc accttcgctc acctgcaatt
     3361 gcggccataa caacggtttt ggtagatctt gtgcggtacg catcacgcct ttgtcatagg
     3421 ccagcgcgac atagcgttcc accattagcg gccaggcttc tttatcgagc acgcgtaaat
     3481 cgttgagtaa accgtagcgc atctcgccgc gccagccggt gcgatgagga atacccgcga
     3541 agaaaggcac taatgcagat ttgaaggagt tgggtaagac gtaggcgcgg tcgtagcgct
     3601 tttcacgcag gctatgaccc agtttgcggc gttcgccgat ttccagcgct ccgtgaccga
     3661 gaggcatagg aatagcttcg ttaacttccg gcatccgcga taataatgga cggcaccatg
     3721 ccggtgccat cacatcgatt atcgcctggg gatagcgcgc ctggagcgtg cgatagagac
     3781 tttgcgacat catcatgtcg ccaacccaag acgggccgat caccagtatt ttcatgcaga
     3841 gctcttatgc gtcgcgattc agccaggcca tgtattccgt tacaccttca gcaacggttt
     3901 tgaacggttt gtcgtaaccc gccgcgcgca gatttgtcag atctgcctga gtgaacgcct
     3961 ggtagcggcc tttcagctta tccgggaacg gaatgtattc gatctggcct ttcttgtgat
     4021 aagccagcgt agcatcagct acagcctgga aggattccgc acgaccagta ccgaggttga
     4081 agatgccgga aacgccattt tccaggaacc acagattcac atcagccacg tcgcccacat
     4141 agacgaagtc gcgtttgaag ttctcgctac cttcaaacag cttcggtgat tcaccgttgt
     4201 taagctgggt gttgagatgg aaagcgacgc tcgccatgct gcctttatgg ccttcacgcg
     4261 gtccataaac gttgaaatag cggaagccaa caatctgcga gttagcttca ggcaggattt
     4321 ggcgaacata ttcatcaaac aggaattttg agtaaccgta gacgttcaac ggtttttcgt
     4381 actcgcggga ttcaataaag tcggaggtgc gtccgccgta ggtggctgcg gaagaggcat
     4441 acaggaacgg gatttcgcgc tccagacagt agtgcagcag ctctttggag tattgatagt
     4501 tgttatccat catatacttg ccatcccact cggtggtgga agagcacgcg ccttcgtgga
     4561 aaatcgcttc gacatcgccg aactcttcgc cagccataat ctggatcagg aagtcttcct
     4621 tatccatata gtccgcgata tccagatcca ccaggttcac aaacttggtg ccgtctttca
     4681 ggttgtccac caccagaata tcggtgatgc ctttatcatt cagggcctta acgatgttgc
     4741 tgccgataaa gcccgcgccg ccggtaacga tgatcataac tgtaaccttc gaattatgga
     4801 gtcagagaca atctcagaca cgaatacttc tatcatatca ctacacggcg ttggcttcag
     4861 ccattcgcca aaagtgagat tgatggacgt tatttatcct gaattttgca gaagtgttaa
     4921 cgcgtcatct cgtcgcgacc tataagtttg ggtaatatgt gctggaattt gccctgtctg
     4981 gagaatcgca atgcgtggag aattttatca gcagttaacc aacgatctgg aaaccgcacg
     5041 ggcggaaggg ttgtttaaag aagagcgcat tattacgtct gcgcagcaag cagatatcac
     5101 tgtggctgat ggaagccacg tcattaactt ttgtgccaat aactatctcg ggctggcgaa
     5161 ttatccggat ctgattgcgg cggcaaaggc gggaatggat tctcacggtt tcggcatggc
     5221 ttcggtgcgt tttatttgcg gcactcagga cagccataaa gagcttgaac aaaaactggc
     5281 ggcattcctg gggatggaag atgcgattct ctactcttcc tgctttgatg ctaacggtgg
     5341 cctgtttgaa acgctgctgg gcgcggaaga cgccattatc tccgacgcac tgaaccacgc
     5401 gtctattatt gatggtgtgc gtctgtgtaa agctaaacgc tatcgctatg ccaacaacga
     5461 tatgcaggag ctggaagcac gtctgaaaga agcgcgtgaa gccggtgcgc gtcatgtgct
     5521 gattgccacc gatggtgtgt tctcgatgga cggcgtgatt gccaacctga agggcgtttg
     5581 cgatctcgca gataaatatg atgccctggt gatggtagac gactcccacg cggtcggttt
     5641 tgtcggtgaa aatggtcgtg gttcccatga atactgcgat gtgatgggcc gggtcgatat
     5701 tatcaccggc acgctcggta aagcgctggg cggggcttct ggtggttata ccgcggcgcg
     5761 caaagaagtg gttgagtggc tgcgccagcg ttctcgtccg tacctgtttt ccaactcgct
     5821 ggcaccggcc attgttgccg catccatcaa agtactggag atggtcgaag cgggtagcga
     5881 gctgcgtgac cgtctgtggg cgaacgcgcg tcagttccgt gagcaaatgt cggcggcggg
     5941 ctttaccctg gcgggagccg atcacgccat tattccggtc atgcttggtg atgcggtagt
     6001 ggcgcagaaa tttgcccgtg agctgcaaaa agagggcatt tacgtcaccg gtttcttcta
     6061 tccggtcgtt ccgaaaggtc aggcgcgtat tcgtacccag atgtctgcgg cgcatacccc
     6121 tgagcaaatt acgcgtgcag tagaagcatt cacgcgtatt ggtaaacaac tgggcgttat
     6181 tgcctgagga tgtgagatga aagcgttatc caaactgaaa gcggaagagg gcatctggat
     6241 gaccgacgtt cctgtaccgg aactcgggca taacgatctg ctgattaaaa tccgtaaaac
     6301 agccatctgc gggactgacg ttcacatcta taactgggat gagtggtcgc aaaaaaccat
     6361 cccggtgccg atggtcgtgg gccatgaata tgtcggtgaa gtggtaggta ttggtcagga
     6421 agtgaagggc ttcaagattg gcgatcgcgt ttctggcgaa ggccatatca cctgcggtca
     6481 ctgtcgcaac tgtcgtggcg gtcgtaccca tctgtgccgc aacacgatcg gcgtcggcgt
     6541 taaccgcccg ggctgcttcg ccgaatatct ggtgattccg gcgttcaacg ccttcaaaat
     6601 tcccgacaat atctccgacg acctggcttc catttttgat cccttcggta acgccgtgca
     6661 taccgcgctg tcgttcgatc tggttggcga ggatgtgctg gtctctggtg caggcccgat
     6721 tggtattatg gcagcggcgg tggcgaaaca cgttggtgca cgcaatgtgg tgatcactga
     6781 tgttaacgaa tatcgtctcg aactggcgcg caaaatgggt atcacccgtg cggtcaacgt
     6841 cgctaaagaa aaccttaatg atgtgatggc agaactgggc atgaccgaag gctttgatgt
     6901 cggtctggaa atgtccggtg cgccgccagc gtttcgtacc atgctcgaca ccatgaacca
     6961 cggcggtcgc attgcgatgc tgggtattcc accgtccgat atgtctatcg actggaccaa
     7021 agtaatcttt aaaggcttgt tcattaaagg tatttacggt cgtgagatgt ttgaaacctg
     7081 gtacaagatg gcggcgctga ttcagtcagg tctggatctc tcgccgatca ttacccatcg
     7141 tttctctatc gatgatttcc agaagggctt tgacgctatg cgttcgggcc agtccgggaa
     7201 agtaattctg agctgggatt aacacgaaca agggctggta ttccagccct tttgcctgag
     7261 gataatccgt taaatatgta aaatcctgtc agtgtaataa agatatcgta attgcgctga
     7321 tctcttatat agctgctctc attatctctc taccctgaag tgactctctc acctgtaaaa
     7381 ataatatctc acaggcttaa tagtttctta atacaaagcc tgtaaaacgt caggataact
     7441 tcagaggtcg tcggtaattt atgatgaaca gcaccaataa acttagtgtt attattccgt
     7501 tatataatgc gggcgatgat ttccgcactt gtatggaatc tttaattacg caaacctgga
     7561 ctgctctgga aatcattatt attaacgatg gttcaacgga taattctgtt gaaatagcaa
     7621 agcattacgc agaaaactat ccgcacgttc gtttgttgca tcaggcgaat gctggcgcat
     7681 cggtggcgcg taatcgtggg attgaagtgg caacgggcaa atatgtcgct tttgtcgatg
     7741 ctgacgatga agtctatccc accatgtacg aaacgctgat gaccatggcg ttagaggacg
     7801 acctcgacgt ggcgcagtgc aacgctgact ggtgttttcg tgaaacggga gaaacctgga
     7861 aatccatccc cagcgatcgc cttcgctcaa ccggcgtatt aaccggcccg gactggctgc
     7921 ggatggggct ttcttcgcgc cgttggactc acgttgtctg gatgggggtt tatcgccgcg
     7981 atgttattgt taaaaataac attaaattta ttgccggatt acatcatcag gatattgtct
     8041 ggacaacaga atttatgttt aatgcgctgc gtgcgcgata taccgagcaa tcgttatata
     8101 aatattatct gcataatacg tcagtgagtc ggttgcatag acaagggaac aaaaacctta
     8161 attatcaacg tcactatatt aagattactc gtctgctaga gaaattaaat cgaaattatg
     8221 ccgacaaaat tacgatttat ccggaatttc atcagcaaat aacttacgaa gcattgcgtg
     8281 tttgccatgc ggtgcgcaaa gagccggata ttattacccg ccaacggatg attgccgaga
     8341 tatttacttc cggtatgtgg taatgactcc aacttattga tagtgtttta tgttcagata
     8401 atgcccgatg ac
//
LOCUS       DAZPEP010000164         8376 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZPEP010000164 DAZPEP010000000
VERSION     DAZPEP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.165"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(113..189)
                     /locus_tag="ACHUT9_003634"
     tRNA            complement(113..189)
                     /locus_tag="ACHUT9_003634"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Asp,seq:gtc)
     gene            complement(322..1053)
                     /gene="dnaQ"
                     /locus_tag="ACHUT9_003635"
     CDS             complement(322..1053)
                     /gene="dnaQ"
                     /locus_tag="ACHUT9_003635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFR1350021.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1118..1585
                     /gene="rnhA"
                     /locus_tag="ACHUT9_003636"
     CDS             1118..1585
                     /gene="rnhA"
                     /locus_tag="ACHUT9_003636"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFR1350022.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1582..2304)
                     /locus_tag="ACHUT9_003637"
     CDS             complement(1582..2304)
                     /locus_tag="ACHUT9_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1350023.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2338..3093
                     /gene="gloB"
                     /locus_tag="ACHUT9_003638"
     CDS             2338..3093
                     /gene="gloB"
                     /locus_tag="ACHUT9_003638"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFR1350024.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3165..4523
                     /gene="mltD"
                     /locus_tag="ACHUT9_003639"
     CDS             3165..4523
                     /gene="mltD"
                     /locus_tag="ACHUT9_003639"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFR1350025.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4572..5195)
                     /locus_tag="ACHUT9_003640"
     CDS             complement(4572..5195)
                     /locus_tag="ACHUT9_003640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1350026.1"
                     /translation="MSGLPQGRPTFGAAQNVSAVVAYDLSAHMLDVVAQAAEARQLKN
                     ITTRQGYAASLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSP
                     GHPVRDIWLQAVEALRDTSHVRNYASGAWLTLINEANLIVDNLITDKLPLEFSSWVVR
                     MRTPEALVDAIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(5199..5999)
                     /locus_tag="ACHUT9_003641"
     CDS             complement(5199..5999)
                     /locus_tag="ACHUT9_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFR1350027.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6240..7154
                     /gene="yafC"
                     /locus_tag="ACHUT9_003642"
     CDS             6240..7154
                     /gene="yafC"
                     /locus_tag="ACHUT9_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFR1350028.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7151..7954)
                     /gene="dkgB"
                     /locus_tag="ACHUT9_003643"
     CDS             complement(7151..7954)
                     /gene="dkgB"
                     /locus_tag="ACHUT9_003643"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFR1350029.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8117..8193)
                     /locus_tag="ACHUT9_003644"
     tRNA            complement(8117..8193)
                     /locus_tag="ACHUT9_003644"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8157..8159),aa:Asp,seq:gtc)
BASE COUNT         2234 a         2021 c         2025 g         2096 t
ORIGIN      
        1 acatttacag cataacaaaa ccatagaccg aagaaatagc gggatttaaa tgcaaaaaaa
       61 ccgcaaaatt aaaaatcttg cggttctctg aactcatttt catgagtgaa tttggcggaa
      121 cggacgggac tcgaacccgc gcccccctgc gtgacaggca ggtattctaa ccgactgaac
      181 taccgctccg cgttgtgttc cgttgggaac gaggcgaata ttacggattg cctcaccctt
      241 cgtcaacggt ttttctcatc ttttgaatcg tttgctgcaa aaatcgccca agccgctatt
      301 tttagcgcct tttacaggta tttatgcccg ccagaggcag cttccgccct tcttctccac
      361 cagatcaaga cgggcttcat gagctgcaag ctcttcatct gtcgcaaaaa caacgcgtaa
      421 cttacttgcc tgacgtacaa tgcgctgaat tgttgcttca ccttgttgct gctgtgtctc
      481 tccttccatc gcaaaagcca tcgacgtttg accaccggtc atcgccagat aaacttccgc
      541 aaggatctgt gcatcgagta atgccccgtg tagcgttcgt ttactgttat ctatttcgta
      601 gcgggcacat aacgcatcga ggctgttgcg cttaccagga aacattttcc tcgccaccgc
      661 aaggctatcg gtgaccttac agaaggtatt ggtcttcgga atatcgcgct taagcaacga
      721 aaactcgtag tccataaagc cgatatcgaa cgctgcgtta tggatcacca actccgcgcc
      781 gcggatatag tccatgaact catcggctac ttcggcaaac gtgggcttat cgagcaaaaa
      841 ttcatcggca ataccatgta cgccaaaggc ttccggatcc accagccgat cgggtttgag
      901 ataaacatgg aagttattgc ccgtcaggcg acggttcacc acttcaacgg caccaatctc
      961 aatgatcttg tggccttcat agtgcgcacc aatctggttc ataccggtgg tttcggtatc
     1021 gagaacgatc tggcgtgtaa ttgcagtgct catagcggtc atttatgtca gacttatcgt
     1081 tttacagttc gattcaatta caggaagtct accagagatg cttaaacagg tagaaatttt
     1141 caccgatggt tcgtgtctgg gcaatccagg acctgggggt tacggcgcta ttttacgcta
     1201 tcgcggacgc gagaaaacct ttagcgcagg ctacacccgc accaccaaca accgtatgga
     1261 gttgatggcc gctattgtcg cgctggaggc gttaaaagaa cattgcgaag tcattttgag
     1321 taccgacagc cagtatgtcc gccagggtat cacccagtgg atccataact ggaaaaaacg
     1381 tggctggaaa accgcagaca aaaaaccagt aaaaaatgtc gatctctggc aacgtcttga
     1441 tgctgcccta gggcaacatc aaatcaaatg ggaatgggtt aaaggccatg ccggacaccc
     1501 ggaaaacgaa cgctgtgatg aactggctcg tgctgcggca atgaatccca cactggaaga
     1561 tacgggctac caagtcgaag tttaagcttg tggtttacga cattgccggg tggctccaac
     1621 cgcctggcga attcgtggct tgtttttact ctgtttcatc gggtttagcg ttaaaggaat
     1681 agtccgtttc cgggcaacaa taagttgtaa gcaaccaagc gcaggaatat gcgcattcaa
     1741 tagttttcct ccgtgtttgt tccacgggag aacgtggaaa cggctggcgt gcagcacttc
     1801 aaaattcaac aaagagagcc agtccagttg ccgcatcaga gtaaacatcc ggctgttata
     1861 gggcgaggtt tttcgcaata ccggcacaag tttgcgtaat cccattaaac taatgggatt
     1921 gaagccacta atcaccagcc agccatcatc aatcaatacc cgatcggttt cacgcaataa
     1981 acgatgcgga tcggtgcacc acggcaatgt atgtgccagt agacaaacat caacggattt
     2041 atcggcaaaa ggaagatgaa gtggatccgc ctgcacctgg acgggcattc cttgcgcaga
     2101 aacattcact tgatgagaaa ccgcgcacgc ttcgcaattg atttctgcgc ttaaattgcc
     2161 aatcttaagc agatgaaaac catacatttt agtgaaccac gggttgagct ggcgctccag
     2221 cgccttgcga taaagctctc cccagggcaa atcgccccag cgatcaggag ccacgacagt
     2281 ttgagggact cttgccggtt tcatcacaac cttccgtttc acactaagag gtaatctatg
     2341 aatcttaaca gtattcccgc ctttgatgac aattacatct gggttttgaa tgatgaagca
     2401 ggtcgctgcc tgattgtcga tcccggagac gcagagccag tattaaacgc cattgccgcc
     2461 aataactggc aaccggaggc catatttctc acccaccatc atcacgatca cgttggcggc
     2521 gtaaaagaac tggtggaaaa gtttccgcaa attgtggtgt atggtccaca agagacacaa
     2581 gataagggaa caacacaggt agtcaaagat ggcgaaactg ccttcgtttt ggggcatgaa
     2641 tttagtgtaa ttgccacacc gggtcacact ttaggacata tctgttactt cagtaaacct
     2701 tatctatttt gcggcgacac gctgttttct ggtgggtgtg gtaggttgtt tgaagggaca
     2761 gcatcacaaa tgtatcaatc acttaataag ttaagtgcgt tacctgacga tacattggta
     2821 tgttgtgctc atgaatatac gttatcaaat atgaagtttg ctttgagtat tcttccgcac
     2881 gatttgtcca taaatgatta ttatcgtaaa gttaaggagt tacgggcaaa aaatcaaata
     2941 acactacccg taattctgaa aaatgagcgg caaattaatg tttttttaag aacggaagat
     3001 attgatttaa ttaatgtaat taatgaagaa acattattgc aacaacctga agagcgtttt
     3061 gcatggttaa ggtcaaagaa agataggttc tgataaaact ttcttgtcat cggctccgtt
     3121 cgccgttatg atcggtcgtc ttttaagcaa ctattgacac acacatgaag gcaaaagcga
     3181 tattactcgc ctctgtcctg ctcgtgggtt gccagagtac cggcaacgtt caacagcacg
     3241 cacagagcct ttctgcagct ggtcaagggg aagcagcaaa gtttacaagt caggcacgat
     3301 ggatggacga tgggacgtct atcgcgccag atggtgactt gtgggctttc attggcgacg
     3361 agctaaagat gggaattccg gaaaatgacc ggattcgcga acagaaacag aaatatttac
     3421 gcaataagag ctatctccac gatgtaactt tacgggcaga gccgtatatg tactggatag
     3481 cagggcaagt taaaaaacgt aacatgccta tggaactggt actactaccc atagtggaga
     3541 gcgcttttga tcctcacgca acgtctggcg ccaatgccgc aggcatctgg cagatcattc
     3601 cgagcacggg gcgcaattat ggtttgaaac agacccgcaa ttatgacgcg cgtcgcgatg
     3661 ttgttgcttc aacaactgcc gcgctgaata tgatgcagcg tctgaacaaa atgtttgacg
     3721 gcgactggct tctgaccgta gcggcttata acagcggcga aggtcgggtc atgaaggcaa
     3781 ttaaaacgaa caaagcgcgt gggaaatcca cggacttctg gtcgttaccg ttgccgcagg
     3841 aaacgaagca gtacgtgcct aaaatgctgg cattgagtga tattctcaaa aacagcaagc
     3901 gttatggcgt acgtctgcca acgaccgatg aaagccgtgc tctggcgcgt gtgcacctga
     3961 gtagcccggt tgaaatggcg aaggttgcag atatggcggg gatttccgtc agcaagctga
     4021 agacattcaa cgctggcgtg aaaggctcca cgctgggcgc aagtgggccg cagtacgtga
     4081 tggtgccaaa gaagcatgca gatcaactgc gtgaatctct cgcttcaggc gaaattgctg
     4141 ctgtacagtc gacgctggtt gccgacaata cgccgcttaa cagccgtgtt tacaccgtac
     4201 gctctggcga cacgctttca agcatcgctt cacgtctcgg cgtaagcacc aaagatttgc
     4261 agcagtggaa caaactgcgc ggatctaagc tgaagccagg ccaaagtttg acgattggtg
     4321 caggcagtag cgcacagcgg ttggcaaaca acagcgatag cattacgtat cgtgtgcgca
     4381 aaggcgattc gctttcaagc attgctaaac gccacggcgt gaacatcaaa gatgtgatgc
     4441 gctggaacag cgacactgcg aatctgcaac caggcgataa gctgacgttg tttgtgaaaa
     4501 acaacaacat gccagattcc tgacaaacca gataataaaa aaggcaccga ttcccccggt
     4561 gcctttttta tttatgccgc tttatgtgct tctaccatga tgatatcact ggtgaaagag
     4621 ccatcagtct gcaaggaaaa atacgttctc acctctgtcg atgcgctctg ttggtaaatg
     4681 cgaatagcgt ctactaacgc ttctggcgta cgcattctca cgacccatga agaaaattcc
     4741 agcggtaact tatctgtaat taaattatca actatcagat tggcttcatt gattaacgtc
     4801 aaccacgcac cgctggcgta gtttcgtacg tgagaggtat cgcgtaatgc ttctaccgcc
     4861 tgtaaccaga tgtcgcgcac tgggtgaccc ggagacatta catccatcac aatcagccta
     4921 ccgccaggtt tcaatatcct attcacttct cgcagtgctg caccaacatc atgccagtga
     4981 tgggcagaat aacggctgat aacaatatca aatgcgttat cggcaaatgg cagacttgcg
     5041 gcatatccct ggcgggtggt gatatttttc agttgccggg cttcggcagc ttgtgccacg
     5101 acatccagca tgtgggcaga taagtcatac gccaccaccg cgctcacgtt ttgcgcagcg
     5161 ccaaacgttg gtctgccctg cggcagacct gacatacctt atttatcagg cttgccggga
     5221 ctgaattcaa cgagtagcgg attgtgatcg gaagcgcgcg taaccagtac agaagcttca
     5281 ctgacgttca gaccacggta gaaaacaaaa tcgagcgggc gaccaaacgc ccgacggcgc
     5341 tgatcatcgg taaaacgcac ctggcgcagc gacatttccc gcgcaaagcg atataacgcg
     5401 ttcatccttc tacggctcca ggcattgaaa tctcccgcca taatgaccgg gccgctgtgg
     5461 tgagctatct gatcaccaat aggaagtaac tgcttactat agacatccac gcccagactg
     5521 aagttgacgg cgtgtatatt aaccaccatc aacaggcggg tgtcaggtaa tggatagacc
     5581 gtcaccagtg ccgacttcgc cagacgcaaa atgggttctc gttcacgtaa cgggcagcaa
     5641 tacactggat gtgccgccga aagggtcatt acgccagaag gatgttgtgg cagcacgaaa
     5701 gcgggtacct gatcggcggc aagatagtta gcggtcgcaa actgtaccaa ctctggcgtt
     5761 gtttgcgctt cctgcaataa caccagatgt gcgtctttgc cgtaattctt taataccgac
     5821 aaccattcag cgcgttgctg tttgtatatg ttccacacca ggatccgaat acgctcttcg
     5881 gtacttaacg gttctccggg tggtaatgcc tggccgatgc tcgcaaaaga ccccggcggt
     5941 aagatcctct ccgcaggttg tccggcaaca tagcgcatgg cataggtgtt ttttcgcact
     6001 tttgactttg aaacctctat tacgtcaacc cttccgcact cgggagggtt ctactgttat
     6061 agggatttta gctcactctt tcaacgagca attgtatatt gttatgtaag caagtgctta
     6121 caccggtcag aaatttgctc attattgaga acattaaatc ggatgacata tcacgcatcc
     6181 tgatggttta atgacaaata aattcaaaat atctcatcta ttttgcaaca ggagcaaaaa
     6241 tgaaagccac gtcggaagaa ctcgccattt ttgtttcggt cgtcgaaagc ggcagcttta
     6301 gccgggcagc ggaacaatta gggcaagcaa actcagcggt aagccgggcg gtgaaaaagc
     6361 tggaaatgaa acttggcgtt agcctgctta atcggaccac gcggcaactt agcctgacgg
     6421 aagaaggcga gcgttatttt cgtcgcgtac agtcaatttt gcaggagatg gcagcggcag
     6481 aatcagaaat tatggagacg cgtaatacac cgcgtggact gttacggatc gatgccgcaa
     6541 ctccagtggt gctgcacttt ctgatgccgt taattaagcc tttccgtgaa cgctatccgg
     6601 aagtcacttt atcgctagtc tcctccgaaa cgattattaa tttgatcgaa agaaaagtgg
     6661 atgtcgcgat acgcgctggt acgttaacgg actccagctt acgtgccagg ccgttattta
     6721 acagttatcg aaaaattatc gcctcccccg attatatttc ccgctacggg aagccagaaa
     6781 cgatcgacga tttaaagcaa catgtttgcc tgggattcac tgaacccgct tccctcaata
     6841 cctggccgat agcctgtagt gatggacaat tacatgaggt gaagtacggt ttgtcatcca
     6901 atagtgggga aacactgaaa cagctttgcc tgagtggcaa cgggattgcg tgtttgtccg
     6961 actacatgat cgacaaagaa atcgctcgcg gagaattggt ggagttaatg gcagataaag
     7021 tgttgccagt ggaaatgcca ttcagtgccg tctattacag cgaccgtgcg gtaagtacgc
     7081 gcatccgggc ttttatcgat ttccttagcg agcatgtaaa aacagctccc ggaggagctg
     7141 tcagagaggc ttaatcccat tcaggagcca gaccttccgg gctaaccagg cggtcgttgc
     7201 aatccagtgc ggcgatcgct tttttatctt cggcatcaag ctgtaaattt tgtgccttaa
     7261 gattactttc caggttttta cgtttagtag aagaaggaat tactgagtaa ccttccccca
     7321 tagcccacgc cagaatcact tgtgccggag tcgcattgtg tttagctgca atacgagcaa
     7381 taacctcatc tttcagtgcc ttaccataag ccagcgtcat ataggaagtg atatgaatac
     7441 cgtgctgttt agcccaggca accactttac ggttttgcag ataaggagag agttcaatct
     7501 ggttggtagc gatgttttca gcgccaacag cagcaatcgc tttttccatc aacgggatcg
     7561 tgaagttgga aataccgatc tcacgtgtca gaccttgttt tttggcctcc agcagcgcct
     7621 gcataaactc ttcaacagag acttcatcgt ttggtgacgg ccagtggatt agcgtcagat
     7681 caacataatc ggtacgcaat ttttgcaggc tctcttttag actcgggatc aatttgtctt
     7741 tgctgagatt ttcaatccag attttagtgg tgatgtagag ttcatgacgt ggcacgccac
     7801 tttctgcaat cgcctgacct actgcggctt cgttatcata gatttgtgca gtatcaattg
     7861 cgcgataacc aagttcaagc gccgttttca cagatgaaat aacaacgtcg tctttgagac
     7921 ggaaagtacc taaaccaaat gcagggatag ccataatatg cctcttttaa tgctattttc
     7981 gttaactgag gtgattatgg gcttcagact gttgcggaaa aagagcataa aatgcagagg
     8041 atttttgcga ttctggcaat aatagatata cagacgaaaa aaaacctcga gcgttaactc
     8101 gaggcttctt aataagtggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca
     8161 ggcaggtatt ctaaccgact gaactaccgc tccaccgaat tcttttacaa ccaccggttt
     8221 tatgaccggc ttactgctta atttgatgcc tggcagttta tgaatcactt cgtgattcac
     8281 ccttcgggcc gttgcttcgc aacgttcaaa agctttcgct tttgtcctac tctcttatcg
     8341 agtagtgaac tgcctcaaag gtcagtgcca ttcttc
//
LOCUS       DAZPEP010000165         8372 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZPEP010000165 DAZPEP010000000
VERSION     DAZPEP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.166"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            78..689
                     /gene="ruvA"
                     /locus_tag="ACHUT9_003645"
     CDS             78..689
                     /gene="ruvA"
                     /locus_tag="ACHUT9_003645"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFR1350030.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            698..1708
                     /gene="ruvB"
                     /locus_tag="ACHUT9_003646"
     CDS             698..1708
                     /gene="ruvB"
                     /locus_tag="ACHUT9_003646"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFR1350031.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(1855..2640)
                     /gene="znuB"
                     /locus_tag="ACHUT9_003647"
     CDS             complement(1855..2640)
                     /gene="znuB"
                     /locus_tag="ACHUT9_003647"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFR1350032.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2637..3392)
                     /gene="znuC"
                     /locus_tag="ACHUT9_003648"
     CDS             complement(2637..3392)
                     /gene="znuC"
                     /locus_tag="ACHUT9_003648"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFR1350033.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3471..4403
                     /gene="znuA"
                     /locus_tag="ACHUT9_003649"
     CDS             3471..4403
                     /gene="znuA"
                     /locus_tag="ACHUT9_003649"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFR1350034.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4419..5741
                     /gene="mepM"
                     /locus_tag="ACHUT9_003650"
     CDS             4419..5741
                     /gene="mepM"
                     /locus_tag="ACHUT9_003650"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFR1350035.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            5861..6832
                     /gene="lpxM"
                     /locus_tag="ACHUT9_003651"
                     /gene_synonym="msbB"
     CDS             5861..6832
                     /gene="lpxM"
                     /locus_tag="ACHUT9_003651"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFR1350036.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(6920..8372)
                     /locus_tag="ACHUT9_003652"
                     /pseudo
     CDS             complement(6920..8372)
                     /locus_tag="ACHUT9_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
BASE COUNT         2254 a         1973 c         2080 g         2065 t
ORIGIN      
        1 tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat ctttgattca
       61 ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc
      121 cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt
      181 tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg
      241 aagacgcgca actgctgtac ggttttaaca ataaacaaga gcgcacgttg tttaaagagt
      301 taatcaaaac caacggcgtc ggcccgaagt tggcgctggc gatcctctcc ggaatgtcag
      361 cgcagcagtt cgttaatgcc gttgaacgtg aagaagtggg agcactggtg aaactgccgg
      421 gcattggtaa aaaaaccgcc gagcgtttaa ttgtcgaaat gaaagaccga tttaaaggtt
      481 tgcacggcga tctctttacg ccagccgccg atctggtgct cacgtcgcct gccagcccgg
      541 caaccgacga tgctgaacag gaagcggttg ccgcgctggt ggcgctgggc tataaaccac
      601 aagaagcaag ccgcatggtg agcaaaatcg ctcgcactga cgccagcagt gaaacattaa
      661 ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat
      721 ttctgccggt accactttgc cggaagatgg ggcagatcgc gccattcgcc ctaaattact
      781 ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc
      841 gaaactgcgc ggcgatgccc tcgatcatct gttgattttt ggtcctccgg ggttgggtaa
      901 aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg
      961 tccggtgctg gaaaaggcgg gcgatctggc agcgatgctc actaaccttg aaccgcatga
     1021 cgtgctgttt attgatgaga tccaccgtct atcgccagtt gttgaagaag tgctgtaccc
     1081 ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat
     1141 taaaattgat ttgccgccgt ttaccctgat tggtgcaacc acgcgcgcag gttcgctgac
     1201 atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga
     1261 tctgcaatat atcgtcagtc gcagcgcacg ctttatgggg cttgagatga gtgatgacgg
     1321 cgcgctggaa gttgctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg
     1381 tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc
     1441 tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt
     1501 gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc
     1561 agccattggc gaagaacgtg aaaccattga ggatgtgctg gagccttatc tgattcagca
     1621 aggctttttg cagcgtacac cgcgtgggcg tatggcgacg acgcgggcgt ggaatcactt
     1681 tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcaccg catccgacat
     1741 tggcagtgtg cctgatgcga cgctattcgc gtcttatccg gcctaccaga gatagcgaac
     1801 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga cagattagct
     1861 ggcctgcttt ttcatcatac tgataataaa taacagtgca gcacatagca ctaccgacgg
     1921 acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc
     1981 caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc
     2041 tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc
     2101 aatcgtcaat gccgtcacca gcatcaacaa caatttcacg cgctgtaatt tcacaccatc
     2161 aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa
     2221 aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc
     2281 cagcaaatca ccgaacaggt aagccatcaa atcaacacga atattagaca tcagactaac
     2341 gaccaccagg cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc
     2401 cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat
     2461 caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc
     2521 atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg
     2581 acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat
     2641 gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atggttatga
     2701 tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc
     2761 ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc
     2821 acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa
     2881 tccagttcac gacgcagttg gtcaataagg tcatataacg ccacctggcc atttacatcc
     2941 acgccttgag tgggttcatc cagcactaat aattgcgggc gatttaacaa tgctcgcgct
     3001 aacagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc
     3061 ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag
     3121 cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca
     3181 tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg
     3241 agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt
     3301 tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc
     3361 gagacatttt ccaaggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga
     3421 aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata
     3481 aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg
     3541 cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg
     3601 atggggtaac agaaacagag gttttacttc ctgacggcgc ttcagaacat gattattcac
     3661 tgcgcccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg
     3721 agatggaagc gtttatgcaa aaaccggtaa gcaaattacc aggagcgaag caggtaacga
     3781 ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg
     3841 atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc
     3901 tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg
     3961 aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat
     4021 tagcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt
     4081 tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc
     4141 attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac
     4201 agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg
     4261 ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga
     4321 atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg
     4381 cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg
     4441 tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg
     4501 ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgcgat gccacgccaa
     4561 ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg
     4621 agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca
     4681 aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca
     4741 gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaactggct gcggccgaca
     4801 aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg
     4861 aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg
     4921 ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc
     4981 tgaaaggtac cgtcgggggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg
     5041 ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa
     5101 aaggcgatga atttgcggtg ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa
     5161 gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgct atccgcgctg
     5221 aagatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc
     5281 cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgtact aatccggtga
     5341 ccggtcgcgt tgcagcacac agaggtgttg atttcgccat gccgcagggt acgccagtgc
     5401 tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg
     5461 tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg
     5521 tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac
     5581 gttcaaccgg gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc
     5641 tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg
     5701 cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc
     5761 ggtacgcagt cagtatcggc tttttttatt tggtgcgggg caagttgcgc cgctacacta
     5821 tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa
     5881 tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg
     5941 gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat
     6001 tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt
     6061 aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga
     6121 tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg
     6181 accggagaaa attcagccgc gcgttgactg gcaagggctg gagatcatcg aagagatgag
     6241 gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc
     6301 catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc
     6361 ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag
     6421 aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt
     6481 acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa
     6541 agcgacgttg cccgcgattg gtcgtttgat gaaagtgtgc cgtgcgcgcg ttgttccgct
     6601 gtttccgatt tatgatggca agacgcaccg tctgacgatt caggtgcgcc caccgatgga
     6661 tgatctgtta gaggcggatg atcatacgat tgcgcggcgg atgaatgaag aagtcgagat
     6721 ttttgttggt ccgcgaccag aacaatacac ctggatatta aaattgctga aaactcgcaa
     6781 accgggcgaa atccaaccgt ataagcgtaa agatctttat cctatcaaat aaaaaaagcc
     6841 tctcaaatta ttgagaggct gtactcgata aaaaatctca atgagtgttc ccactatttt
     6901 gagtttttag gttagtacgc tattgagtta atcgtagcgt gataatttca aaaggtttga
     6961 actcaagatt gcaatattga ttgcaataaa aactgtcatc taacttattt tcttgaagat
     7021 cgacatattg aacgtagtga taattacata ctcacgaaag catggctgaa atttctaatc
     7081 catgactctc atataatctt acgattatac cgtcgccgtc ttcagctaat tttattgttt
     7141 cgatgataac gctgttagta tcgatagcaa aaaatgatag tgaagggggt aattcacccg
     7201 ggtgcggttt gacagggacg acctgtaagg gatgattcat tatataggct tgattaataa
     7261 caccacccac acggtaatct ccatgatgag gaaataagga gaatatgaat ttttgtttac
     7321 caatatctgc aaaattatta atcaggaaat tatcataatt gtctgtctta acaacttccc
     7381 caggtttatg ctgcgaacgc agcaaagtta attcaagcgt ctggtctttg acacgatagc
     7441 catatttgtt ttttgctatt aaagctatgc cagtttctcg atcagaaagg tcgacccagt
     7501 gatgtgctgg tatttcatct ttggcaaaag cgaacgaatc atcgctatgc gttggtctgt
     7561 caattacacc gaactgaatt tgacattgtg caagagatgt ttgtactgac accgggaatt
     7621 ttactttgag agaaacatta ggttcgtgcc aatcaataaa cgtttggaca tcaacgcgtg
     7681 gactatcctg tgtcagggta aaggctaaac gaagataaga ggaattgtag tgataaatca
     7741 gcgtcatcgt agttttaggc ccgctgatca cagttgtcat tttttcagcg agtaaaacat
     7801 atgattcccc atcacgataa tttgaagcaa aatcccaggc cgcgaaaaaa cctgcatctt
     7861 cgtggtatgc acggatctca ctgtgcatat tttcagtaat gtattcttta ccatatcttt
     7921 tatcatatag cgaaattaaa tcaccgcttt ctgaaaaaag aagtttaatg taattatttt
     7981 caatactttg agattctgcc ttcagaccat ttacgatctt attctttgga acaatcacag
     8041 cataaccata agagtttact cgtgctttga gccagttgtt gtttatattg atccattcgt
     8101 tacgagcaaa acaggtggta ttaaacaaaa tgtgcggatc tttataagaa gaagtgtcaa
     8161 ttttacttaa taaagtagac tgcgcatcag aaataatttt ctcggttttt gcttctagtt
     8221 taagatattc cttttcagtt tcatgataaa ctcttgaaat acatgagccg ggaaggatgt
     8281 catggaattg caaggtcagc atcgctttcc atatttcatc aaactcactg cgaagaattg
     8341 atgtcatatt attagtaata gttataaaaa at
//
LOCUS       DAZPEP010000166         8347 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZPEP010000166 DAZPEP010000000
VERSION     DAZPEP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.167"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(491..1687)
                     /gene="hemY"
                     /locus_tag="ACHUT9_003653"
     CDS             complement(491..1687)
                     /gene="hemY"
                     /locus_tag="ACHUT9_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFR1350037.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1690..2931)
                     /gene="hemX"
                     /locus_tag="ACHUT9_003654"
     CDS             complement(1690..2931)
                     /gene="hemX"
                     /locus_tag="ACHUT9_003654"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFR1350038.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQVVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPAPQGDTPAAAPQGE"
     gene            complement(2953..3693)
                     /gene="hemD"
                     /locus_tag="ACHUT9_003655"
     CDS             complement(2953..3693)
                     /gene="hemD"
                     /locus_tag="ACHUT9_003655"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFR1350039.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3690..4631)
                     /gene="hemC"
                     /locus_tag="ACHUT9_003656"
     CDS             complement(3690..4631)
                     /gene="hemC"
                     /locus_tag="ACHUT9_003656"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFR1350040.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            5018..7564
                     /gene="cyaA"
                     /locus_tag="ACHUT9_003657"
     CDS             5018..7564
                     /gene="cyaA"
                     /locus_tag="ACHUT9_003657"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFR1350041.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7604..7924)
                     /gene="cyaY"
                     /locus_tag="ACHUT9_003658"
     CDS             complement(7604..7924)
                     /gene="cyaY"
                     /locus_tag="ACHUT9_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFR1350042.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8086..8228)
                     /locus_tag="ACHUT9_003659"
                     /pseudo
     CDS             complement(8086..8228)
                     /locus_tag="ACHUT9_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
BASE COUNT         1866 a         2183 c         2170 g         2128 t
ORIGIN      
        1 gcgcagttga tatgtaaggc aggtttatta tattgcgttg attattaatg cttttttagt
       61 tttaacggca attaatatat gtgttattaa ttgaatgaat tttatcattc ataataagta
      121 tgtgtaggat caagctcagg ttaaatattc actcaggaag caaagaggat tacagaatta
      181 tctcataaca agtgttaagg gatgttattt cccgattctc tgtggcataa taaacgggta
      241 gatgctcatt ccatctctta tgttcgcctt agtgcctcat aaactccgga atgacgcaga
      301 gccgtttacg gtgcttatcg tccactgaca gatgtcgctt atgcctcatc agacaccatg
      361 gacacaacgt tgagtgaagc acccactgtt gtcatacaga cctgttttaa cgcctgctcc
      421 gtaataagag caggcgtttt tttatgcatc agaaaggtcc cggaggtgct tgcctccggg
      481 tgagaaggaa ctactgtggt gggttgttct gcaacgttaa catcaaaccg tcgcgacgca
      541 tcgctgcagc ttcttccggc ttgtgcagtc tgtccagcgc gtcggcaagc catgcgtaat
      601 cgtaggcgtc cggacgttgt ttcagcgctg cgcggaaggc gagcgatgct tcctgccatt
      661 ctccgtgctt catcagtgac tggcccagtg tgctccacaa cagcgggcga tcgccgacgt
      721 ttttgatctg ctggcgcagc actttttcca gctgttccgg attgttggtt ttcagtcgag
      781 gaatcggcag cagtaggcga tcgtcgtact ggcgtttcag gccatcgatg ataatttgct
      841 gggcagtatc atgatcgtca cattcaataa gatgttccgc cattgccacc tgcaacgcta
      901 cctgatgacg cgttttccgg ctttggtttt tccaccagtt acgcaaacct tcactaccgt
      961 tatcggcacg cgcctgatcc atcaggccaa tccatgcctg ttgttccagc attgcacgat
     1021 gttcttcatc accaacatgg gctttcgcca ttgatgggat gatatccagc agagaactcc
     1081 atgcacctgt gcggatatac gcctgttccg ccagacgtaa tacttccgga tggcgtggcg
     1141 taacttccag cagcttatcc acgccgtggc gtgcagcatg gttttcatta cgggccagtt
     1201 gcagacgtac acgggtgatt tctaccggaa tggtgtcatt gccggccagc tctgctgcgc
     1261 gttccagatg ttggttggcg cgtgcttcat caccacgttg ttgcgcggct tcggcagcca
     1321 gtagatagtt caccaccggt tgttccgcgt gatcggcatt tttcgccatc agcttttcaa
     1381 cttgctgata atcgccttcc gccagtttca gcagcgcctg ttcggtctgc ttacgggcac
     1441 ggcgacgctt acgtccgaca aaccacccac gggtgtgtgc gcctgtgcgg aagatccgcc
     1501 gcagtagcca ctcaatggca aacagcacta ccatcgccag aatcaaaata atcgccaggc
     1561 ccgtgacgct ggtttcgata ttgtagttgt cagtctggat cagcacgtaa ccctgatgac
     1621 cggcaatcat cgggccaacc actatccccg caatcagcaa aacaaagagt aataacactt
     1681 ttagcatggt tattctcctt gcggcgcggc tgccggagta tcaccttgcg gtgcaggtgc
     1741 aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc
     1801 aggtttagct tccgttgtcc ccgctgccgg ttgtgccagc aggttacgca cgcgggtctg
     1861 catcagtttt tccagcatcg cctggctttg cagggtttcc ggaagatcca tcgagatatt
     1921 ttgctggctt aactggtcca cctcgtcgag gaacgctttg gtggtggcat catcagtatc
     1981 gtagtaagca cgtacccagg tggagacgtt ctccagcgcc tggcgataag tctcttcctg
     2041 atgacgcggt acagcttgtg ctgcgaccag caggcgagag cgaatatttt cgcgcagata
     2101 gatatcctga tttggcgcta acagcggtac ggcggtgtca tcacgacggc gaatcgtaat
     2161 gaagttgtcc ataaagttct gccagctttt ttgcagattg atacgccatt cgctgatgga
     2221 actggaaagc tcttcaccgt ctgaatccat cggcgaacca tcgctgtcat tatcggccag
     2281 acgcaggtta tctacctgat ttgaaagctg attaagctta aggatgatgc cgtcataatc
     2341 cacctgcgat actgcagaaa ggctggcgat atcatcggta attgcccgac gaacggtaat
     2401 cagactcggg tcattcatat ccgccaggct ggcgtctgca cttttcagca acgctgcagc
     2461 ggtcgtgacg tcctgatcgc tccacagctt ccgtccggcg agtttcacca gaaaatcggc
     2521 ctgggccagc agccaggttt tagcatcgct gccggaaatg gtggcgacct tttgctggac
     2581 ttcatccagc tgttttgcca gcgtttcttg ctgacgattc gcctgttcaa gctgcgtagc
     2641 ctgttgctta ataatgcctt ccagttcggc tttttggctc tcctgggctt tttgcaatgc
     2701 cgtcagttgg ttagccaggg catcgctggt ggcggtctga ttgacgacct gttgtttacc
     2761 ccagccatac aaaccgacgc ccgccgccag agcaatagcg atagccgccg cgctgagaat
     2821 caatgcggta ttgttcttac tctttttttc tgttgcgaca ggttgtgacg tggtgtccac
     2881 ggcctcccta gtctcttcaa ccacggcgga ggttttttct tgttccgtca ttatggcttc
     2941 ctgttatgag agttattgta atgcccgtaa aagcgcatcg ttggcagcgt tatcggcgac
     3001 cttaatgtct tgccagccca gttcccgggc gagtttcgcc aaacgctcac tgacgaccaa
     3061 tagtcgacag tgtagtaacc agtgctcacg ataccattgt gggatcagcg accagagttg
     3121 ctgcaacatt tcaccgctgg taacaacgac cgtcgtcacc tcgcgggatt gccagcgcat
     3181 cgcttcttct gcaccatcgt aatggattgc gcatcgttga taacattcac aaaaagtgac
     3241 ctcagcaccg cgcgccgtca gggtatcccc aattagctca cgaccgccat tgccacgtaa
     3301 tatcagcgca cgtttgcccg caatattttg taattcaggt aattgtagca agacttcgct
     3361 gatttcccga tcctgcgggt agagaatctt ctgtccgctt acggtatgta gtgccagtgc
     3421 ggtggtgcgt ccaatggcga aataattagg tagtcggggc catttacgat cttgctgatg
     3481 tagctgtgat tgggcaaaag caaccgcgtg ttgcgagagg gcaaacaaca gatcgctctc
     3541 ccccagcgct gccagttgat cagcaagttg cggtaattgt tgacccggag aaaactcaat
     3601 cagcggaaaa tgccaggcca cctgccccag tgtgcgcaga cggctcacta actcttctcc
     3661 agcgggagac gggcgggtga caaggatact catgccgggg cgtctccgtt atagacttca
     3721 gcgaggatct cgcgcgcgcc gttattcagt agctcttctg ccagcgaaat ccccatttgt
     3781 ttggcatttt gcggcgcacc gcggcgttca ccgcgaataa tctgcgaacc gtccggcgcg
     3841 ccgaccagcg cacgcagcca gatttcgcca tcaataagct cggcgtagct accaattggc
     3901 acctgacatc cgccttcgag acgggtattc atggcgcgtt ctgcggtaac gcgcagtgca
     3961 gtttcgtggt gattcagcgc ggcaagcagc tcgcgagtgc gcgtatcatc aaggcggcat
     4021 tcaataccga ctgcaccttg tcctaccgcc ggaagagaaa tctcgggtgg taacgcggcg
     4081 cgaatgcggg actccagacc taaacgtttt agtccggcta cggcaagaat gatggcatcg
     4141 tattcgccgt tatctagttt gctcaggcga gtgccgacgt tgccgcgcag ggagcggata
     4201 atcagatccg ggcggcgttc agccagttgg cactggcgac gtaaactgga cgtcccgacg
     4261 atactgcctg ctggtaacgc atccagactg tcatagttat tggacacaaa ggcatcgcga
     4321 ggatcttcac gctcacaaat agtgaccagt cccagacctt gcgggaattc aaccggcaca
     4381 tctttcattg aatgtacggc gatatcggcg cgattttcga ggagcgcgac ttccagctct
     4441 tttacaaata agccttttcc gcctactttc gccagcggcg tatcaagaat cacatcgccg
     4501 cgcgtcacca tcggtaccag ttcaacgacc aggcccggat ggctcgccat caacttgtct
     4561 ttgacatagt gtgcctgcca gagtgcaagt gggctttggc gtgtggcaat tcttaaaaca
     4621 ttgtctaaca tgcttgttac cgtcattatc atccgtggtc catcctaaca tccttgccag
     4681 agtgatgtca gtgttgtggt gaaacgtaga cgcctgcgca aaccgtaaaa tgaggtctgg
     4741 cagtggatcc tgacagacgt ttcacgctgt cgtaataagg aatttacaga gaataaacgg
     4801 tgctacactt gtatgtagcg catctttctt tacggtcaat cagcaaggtg ttaaattgat
     4861 cacgttttag accatttttt cgtcgtgaaa ctaaaaaaac caggcgcgaa aagtggtaac
     4921 ggttaccttt gacatacgaa atatcccgaa tgccgcgtgt taccgttgat gttggcggaa
     4981 tcacagtcat gacgggtagc aaatcaggcg atacgtcttg tacctctata ttgagactct
     5041 gaaacagaga ctggatgcca taaatcaatt gcgtgtggat cgcgcgcttg ctgctatggg
     5101 gcctgcattc caacaggtct acagtctact gccgacattg ttgcactatc accatccgct
     5161 aatgccgggt taccttgatg gtaacgttcc caaaggcatt tgcctttaca cgcctgatga
     5221 aactcaacgc cactacctga acgagcttga actgtatcgt ggaatgtcag tacaggatcc
     5281 accgaaaggt gagcttccaa ttactggtgt atacaccatg ggcagcacct cgtccgtagg
     5341 gcaaagttgt tcctctgacc tggatatctg ggtctgtcat caatcctggc tcgatagcga
     5401 agagcgccag ttactacaac gtaaatgcag cctgctggaa agctgggccg cctcgctggg
     5461 tgtggaagtc agcttcttcc tgattgatga aaaccgcttt cgtcataatg aaagcggcag
     5521 cctggggggc gaagattgtg gctccaccca gcatatactg ctgcttgacg aattttatcg
     5581 taccgccgtg cgtctcgccg gtaagcgtat tctgtggaat atggtgccgt gcgacgaaga
     5641 agagcattac gacgactatg tgatgacgct ttacgcgcag ggcgtgctga cgccaaatga
     5701 atggctggat ctcggtggct taagctcgct ttctgctgaa gagtactttg gtgccagcct
     5761 ttggcagctc tacaagagta tcgattcccc atacaaagcg gtgctgaaaa cactgctgct
     5821 ggaagcctat tcctgggaat acccgaaccc acgtctgctg gcgaaagata tcaaacagcg
     5881 tttgcacgac ggcgagattg tatcgtttgg cctcgatcca tactgcatga tgctggagcg
     5941 tgttactgaa tacctgacgg cgattgaaga tttcacccgt ctggatttag tacgtcgctg
     6001 cttctattta aaagtgtgcg aaaaactcag ccgtgaacgc gcctgtgtag gctggcgtcg
     6061 cgcagtgttg agccagttag tgagcgagtg gggttgggac gaagctcgtc tggcaatgct
     6121 cgataaccgt gctaactgga agattgatca ggtgcgtgag gcgcacaacg agttgctcga
     6181 cgcgatgatg cagagctacc gtaatctgat ccgctttgcg cgtcgcaata accttagcgt
     6241 ctccgccagt ccgcaggata tcggcgtgct gacgcgtaag ctgtatgccg cgtttgaagc
     6301 attaccaggt aaagtgacgc tggtaaaccc gcagatttca cccgatctct cggaaccgaa
     6361 tctgaccttt atttatgtgc cgccgggccg ggctaaccgt tcaggttggt atctgtataa
     6421 ccgcgcgcca aatattgagt cgatcatcag ccatcagccg ctggaatata accgttacct
     6481 gaataaactg gtggcgtggg catggtttaa cggtctgctg acctcgcgca cccgtttgta
     6541 tattaaaggt aacggcattg tcgatttgcc taagttgcag gagatggtcg ccgacgtgtc
     6601 gcaccatttc ccgctgcgct tacctgcacc gacaccgaag gcgctctaca gcccgtgtga
     6661 gatccgccat ctggcgatta tcgttaacct ggaatatgac ccgacagcgg cgttccgcaa
     6721 tcaggtggtg cattttgatt tccgtaagct ggatgtcttc agctttggcg agaatcaaaa
     6781 ttgcctggta ggtagcgttg acctgctgta ccgcaactcg tggaacgaag tgcgtacgct
     6841 gcacttcaac ggcgagcaat cgatgatcga agccctgaaa actattctcg gcaaaatgca
     6901 tcaggacgcc gcaccgccag atagcgtgga agtcttctgt tatagccagc atctgcgcgg
     6961 gttaatccgt actcgcgtgc agcaactggt ttctgagtgt attgaactgc gcctttccag
     7021 cacccgccag gaaaccgggc gtttcaaggc gctgcgcgtt tctggtcaaa cctggggctt
     7081 gttcttcgaa cgcctgaatg tatcggtaca gaagctggag aacgccatcg agttttatgg
     7141 cgctatttcg cataacaaac tgcacggcct gtcagtgcag gttgaaacca atcacgtcaa
     7201 attaccggcg gtggtggacg gctttgctag tgaagggatc atccagttct tcttcgaaga
     7261 aacgcaagac gagaatggct ttaatatcta cattcttgac gaaagcaacc gggttgaggt
     7321 gtatcaccac tgcgaaggca gcaaagagga actggtgcgt gacgtcagtc gcttctactc
     7381 gtcatcgcac gaccgcttca cctacggctc aagcttcatc aacttcaacc tgccgcagtt
     7441 ctatcagatt gtgaaggttg atggtcgtga acaggtgatt ccgttccgca caaaatctat
     7501 cggtaacatg ccgcctgcca atcaggatca cgatacgccg ctattacagc aatatttttc
     7561 gtgatgaacg tgccggaaag cgaggcttat ccggcatgca atcttagcgg aaactgactg
     7621 tttcacccgc ctgctgcgtc gccgcctgtt ccagcaaatc ccagaaggtt tcgccgctgc
     7681 gatcgcaaat ccactcatcg cctttcagat caaaatggta gccgccttgt ttggttgcca
     7741 gccatacctg gtgcagcggt tcctggcggt tgataatgat tttgctgccg ttttcaaagg
     7801 taatggtcag cacgccgccg ttgatttcgc agtcgatatc gctgtcgcca tcccagtcgt
     7861 ccaggcgttc ttcaatggtc agccagagtt gatcagccag gcgatgaaat tcactgtcgt
     7921 tcattgttgt atcctgtttt taagtgatgg cggcagtata gcggcatggg gtcagggctt
     7981 caaagtttgc acctctgcgg ctgcgttccg gcacgattca tccgtcaccg gaataatgat
     8041 gtctctgtgt agcgaaagat ttgtctcttc attagggcgc agttacacca cgcctttccc
     8101 tgtttctggt aaacattatg atcaggttta ccgagcgagc atcctcacgc tgacggaact
     8161 aaaaaagaca acaaacaaac cacattgcga tagtgcatag agccatcctg gcgcgaggtg
     8221 ccgatcacga aactaccagc aaaacataaa tccccacgag taagcgttat actcgcagca
     8281 tttcctcact tttcagactt cataaagagt cgctaaacgc ttgcttttac gtcttctcct
     8341 gcgatga
//
LOCUS       DAZPEP010000167         8217 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZPEP010000167 DAZPEP010000000
VERSION     DAZPEP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.168"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..111
                     /gene="yjbL"
                     /locus_tag="ACHUT9_003660"
     CDS             <1..111
                     /gene="yjbL"
                     /locus_tag="ACHUT9_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFR1350043.1"
                     /translation="IDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            135..842
                     /locus_tag="ACHUT9_003661"
     CDS             135..842
                     /locus_tag="ACHUT9_003661"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFR1350044.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            1205..2242
                     /gene="dusA"
                     /locus_tag="ACHUT9_003662"
     CDS             1205..2242
                     /gene="dusA"
                     /locus_tag="ACHUT9_003662"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFR1350045.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2376..2618
                     /gene="pspG"
                     /locus_tag="ACHUT9_003663"
     CDS             2376..2618
                     /gene="pspG"
                     /locus_tag="ACHUT9_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFR1350046.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2784..3767)
                     /locus_tag="ACHUT9_003664"
     CDS             complement(2784..3767)
                     /locus_tag="ACHUT9_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFR1350047.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3850..5265
                     /gene="dnaB"
                     /locus_tag="ACHUT9_003665"
     CDS             3850..5265
                     /gene="dnaB"
                     /locus_tag="ACHUT9_003665"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1350048.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5318..6397
                     /gene="alr"
                     /locus_tag="ACHUT9_003666"
     CDS             5318..6397
                     /gene="alr"
                     /locus_tag="ACHUT9_003666"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFR1350049.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6650..7843
                     /gene="tyrB"
                     /locus_tag="ACHUT9_003667"
     CDS             6650..7843
                     /gene="tyrB"
                     /locus_tag="ACHUT9_003667"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFR1350050.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         2021 a         1923 c         2102 g         2171 t
ORIGIN      
        1 attgattatg cagcgctgcg tcatcgggat ataattattg caaaaataga tttgtttatt
       61 caacgaatca cgcataattt atggcatgca cgtaaaaaaa tgtgttttta attgagcaaa
      121 taaatgatct caaaatgtgg gtcaataagt atattgacga ttgcactgat gaggatttaa
      181 acgatcgtga ctttattgca tcagttgttg accgggctat ttttcatttc gcgattaata
      241 gtatatgtaa tcctggggat aataaagatg cgactcccat tgaacaatgt acttttgatg
      301 tagaaactaa gaatggcctt ccctccacgg ttcagctatt ttatgaggaa tctaaggata
      361 atgaaccttt agcgaatata cattttcaag caataggttc tggtttttta acgtttgtta
      421 atgcctgcca ggaacatgat gacaacagct taaaattatt tgcttcgctg ttaatttcac
      481 tttcatattc tagtgcctac gcagatttat cagaaacagt gtatattaat gaaaataatg
      541 agagctacct gaaagctcag tttgaaaaat tatctcaacg tgatatgaag aagtacctgg
      601 gagagatgaa gcgtctggct gatgggggag aaatgaattt tgatggctat ctggataaga
      661 tgtcacatct ggtgaatgaa ggaacgctcg atcctgatat tttaagcaaa atgcgagatg
      721 ctgcaccaca attaattagc ttcgcgaagt cgtttgaccc aacctcaaag gaagagatta
      781 aaatacttac agacacttct aaattaattt atgatttgtt cggggttaaa tcggagaaat
      841 aatatgtgaa gttcttcgat agtatggaag gcattatata aaaggaccca atatttattg
      901 ggttcttttt tctctatcaa tgctattagc agggagatat atcaccagag tttaatgtgt
      961 gattttttat ttatcgtcga acctggattg tttatcattg gccttaacaa agttaacggc
     1021 taataagatt atttccatca cttcgtgaga gcttcatgcc ttgagaggat ctcaattttc
     1081 ttttgcaatg agacaggcgc ttcctgttgt tatggtatag taccccgcta ttgagcctcc
     1141 tgaatagtga tgctgaataa cataacccca tgatatatcg ataaaataat ctctacattt
     1201 gaaaatgcac ggtaattctg aaatgcaaaa aatcaaccaa accagcgcaa tgcctgaaaa
     1261 aactgacgtt cactggagtg gtcggtttag cgttgcacca atgctcgact ggacggacag
     1321 acattgccgc tatttcttgc gtctgctttc ccgcaatacg ttgctgtata ccgaaatggt
     1381 gaccacaggg gcgattattc acggtaaagg tgattacctg gcgtacagtg aagaagaaca
     1441 tccggtagcg ttgcaactcg gcggtagcga tccggcggcg ctggcacagt gtgcgaagct
     1501 ggcagaagcg cgtggatatg atgagatcaa cctgaatgtc ggctgcccgt ctgaccgggt
     1561 gcagaacggc atgtttggtg cgtgtctgat gggtaatgcg cagctggttg ccgactgcgt
     1621 gaaagcgatg cgcgatgtgg tgtcgattcc agtgacggtg aaaacgcgta ttggcatcga
     1681 tgaccaggac agctatgaat ttctctgcga tttcatcaac accgtttccg gcaaaggcga
     1741 gtgtgagatg tttatcatcc acgcacgtaa agcctggctt tcggggttaa gcccgaaaga
     1801 aaaccgtgaa atcccgccgc tcgattatcc gcgtgtgtat caactgaagc gtgactttcc
     1861 gcatctgaca atgtcgatta acggtggtat caagtcgctg gaagaggcca aagcacacct
     1921 gcaacatatg gatggcgtga tggtcgggcg cgaggcgtat cagaatccgg gtattctggc
     1981 ggcggtagac cgggagatct ttggttcctc ggataccgat gccgatccgg tggcggtagt
     2041 gcgcgccatg tatccgtaca ttgagcgtga actcagccag gggacgtatc tcggccatat
     2101 tacccggcat atgttgggct tgttccaggg tattcctggc gcgcggcagt ggcggcgtta
     2161 tttaagtgaa aatgcccata aagcgggtgc agacattaat gtgctggaac acgcgctcaa
     2221 actggtggcg gataagcgtt aacttttcac caaaaagtag tcaaattcac cacgccctgc
     2281 gcaccgtcgc ggggcgtttt gctgttaaat caatagatta tttttggcat gattcttgta
     2341 atgccagcaa gagatttcat atttgggaga gcatcatgct ggaactactt tttgtgattg
     2401 gcttttttgt catgctgatg gtcaccggcg tttcgttgct gggcattatc gccgcgctgg
     2461 ttgtggcgac ggccattatg ttcctcggcg gtatgctggc attgatgatt aagttgctgc
     2521 cgtggttact actggcgatt gcggtggtgt gggttattaa ggcgattaaa gcaccaaaag
     2581 tgccgaaata tcagcgttat gaccgctggc gttactaagg gattgtgcgg atgatcacaa
     2641 cctaaggttt tatccttaga acaaaatagg aattgataat caaatctgtc actattgcgc
     2701 ctctaacaga ttcatcgtgc tgtaccctac atacagccga actataaaaa gaaagggctt
     2761 cccaggtgga agccctattt cttttatgga atcaacaggc tggaaccttg cgtcgcccgg
     2821 ctttccagaa tctcatgcgc acgctgcgca tccttcagcg gatatttctg ctgctcggcg
     2881 acatcgacct taatcacacc gctggcaatc aaagagaaca gttcattact ggcctcggtt
     2941 aattcctccc gcgtggtgat atagccttgc agggaagggc gtgtcacata caacgagcct
     3001 ttttgattga gaatgcctaa gttcacaccg gtaaccgcac ctgatgagtt gccaaaactg
     3061 accattaagc cgcggcgttg caggcaatcc agcgaccgtt cccaggtgtc tctgcccacg
     3121 gaatcgtaca ccacgcgcac tttcttaccg ccggtgatct cttttaaccg ctcgaccaga
     3181 tcctcttcac gatagttaat aacctgccac gcgcccgctt ttagcgcgct ctgcgctttt
     3241 tgcgcggttc ctacggtgcc gataagtttc gcgcccaggg cttttgccca ctggcaggca
     3301 attaagccaa cgccgccagc cgctgcgtgg aacaggaact gttcatcggg tttaatttca
     3361 taggttttgc gcagcagata ataaaccgtt aagcctttca ggaaggatgc cgcagcttgc
     3421 tcaaaagaaa ttgccgcagg cagaatcgcc gctttatccg cattaatgtt atgcacagag
     3481 ctgtaagcgc ctaacgccga ctgcgcatag actacacgat cgcctgcctt aatatgcttt
     3541 acaccactgc cgactttact cacgatgcct gccgcttcgg tacctaatcc gctgggtagt
     3601 gatggcggcg ggtaaaggcc gctgcggata tacgtgtcga taaaattgat gccgatggct
     3661 ttattttcga cctggatttc attctctgcc ggatcggcag gagtgaactc tacggcttga
     3721 agtacttccg ggccaccgtg cttgtgaaat tcaattcgtg ttgccatgtg tcctccagaa
     3781 cgtatcgtca gggtctgctt catatggtaa agtttcgacc cattctttat ctcggtaact
     3841 ccattcacta tggcaggaaa taaacccttc aacaaacagc aggctgaacc ccgcgaacgc
     3901 gatccacaag ttgccgggct gaaagtgcct ccgcactcga tcgaagcgga gcagtcggtg
     3961 ttgggcggtt taatgctgga taacgaacgc tgggacgatg tagccgagcg tgtggtggca
     4021 gacgattttt acacccgccc acaccgccat atctttactg aaatggcgcg tttgcaggaa
     4081 agcggtagtc ctatcgatct gattaccctt gcggaatcgc tggaacgcca ggggcaactc
     4141 gatagcgtcg gtggttttgc ttatctggca gagctgtcaa aaaatacgcc aagtgcggcg
     4201 aacatcagtg cttatgctga catcgtgcgt gaacgtgccg ttgtccgtga gatgatctcg
     4261 gttgcgaatg agattgctga agccggtttt gatccgcagg ggcgtaccag cgaagatctg
     4321 ctggaccttg ctgaatcccg cgtctttaaa attgccgaaa gtcgtgcaaa caaagacgaa
     4381 gggccgaaga acatcgccga tgtgctcgac gcaaccgtgg cgcgtattga gcagttgttt
     4441 cagcagccac acgatggcgt taccggagta aacaccggtt atgacgatct caacaaaaaa
     4501 accgctggct tgcagccgtc ggatttgatc atcgtcgccg cgcgtccgtc gatgggtaaa
     4561 acaacatttg cgatgaacct cgtcgaaaac gcggcgatgt tgcaggataa accagtactt
     4621 atcttctcgc tggagatgcc ttcagaacag attatgatgc gttctctggc gtcgctgtcg
     4681 cgcgttgacc agactaaaat ccgtaccggg cagctcgatg atgaagactg ggcgcgcatt
     4741 tccggcacca tgggtatttt gctcgaaaaa cgcaatatct atatcgatga ctcctccggc
     4801 ctgacgccaa cggaagtgcg ttcccgcgca cgccgtattg cccgtgaaca cggcggcatc
     4861 gggcttatca tgatcgacta cctgcaactg atgcgcgtac cggcgctttc cgataaccgt
     4921 acgctggaaa ttgcagaaat ctctcgctcg ctgaaagcac tggcgaaaga actgaacgtg
     4981 ccggtggtgg cgctgtccca gttgaaccgt tctctggaac aacgtgccga caaacgcccg
     5041 gtcaactccg acctgcgtga atctggctct atcgagcagg atgcggactt gattatgttt
     5101 atctatcgtg atgaggtgta tcacgaaaac agtgatttaa aaggcatcgc ggaaattatt
     5161 atcggtaaac aacgtaacgg cccaatcggg acggtacgcc tgacctttaa cggtcaatgg
     5221 tcgcgcttcg acaactatgc ggggccgcag tacgacgacg aataataatt attttatgaa
     5281 ttaggtaatt aaagcaaaca cttatcaagg aacacaaatg caagcggcaa ctgttgtgat
     5341 taaccgccgc gctctgcgac acaacctgca acgtcttcgt gaactggccc ctgccagtaa
     5401 aatggttgcg gtggtgaaag cgaacgctta tggtcacggt cttcttgaga ccgcgcgaac
     5461 gctccccgat gctgacgcct ttggcgtagc ccgtctcgaa gaagctctgc gactgcgtgc
     5521 ggggggaatc accaaacctg tactgttact cgaaggcttt tttgatgcca gagatctgcc
     5581 gacgatttct gcgcaacatt ttcataccgc cgtgcataac gaagaacagc tggctgcgct
     5641 ggaagaggct agcctggacg agccggttac cgtctggatg aaactcgata ccggtatgca
     5701 ccgtctgggc gtaaggccgg aacaggctga ggcgttttat catcgcctga cacagtgcaa
     5761 aaacgttcgt cagccggtga atatcgtcag ccattttgcg cgcgcggatg aaccaaaatg
     5821 tggcgcaacc gagaaacaac tcgctatctt taataccttt tgcgaaggca aacctggtca
     5881 acgttccatt gccgcgtcgg gtggcattct gctgtggcca caatcgcatt ttgactgggt
     5941 gcgcccgggc atcattcttt atggcgtctc gccgctggaa gatcgctcca tcggtgccga
     6001 ttttggctgt cagccagtga tgtcactaac ctccagcctg attgccgtgc gtgagcataa
     6061 agccggagag cctgttggtt atggtggaac ctgggtaagc gaacgtgata cccgtcttgg
     6121 cgtagtcgcg atgggctatg gcgatggtta tccgcgcgcc gctccatccg gtacgccagt
     6181 gctggtgaac ggtcgcgaag taccgattgt cggacgcgtg gcgatggata tgatctgcgt
     6241 agacttaggt ccacaggcgc aggacaaagc cggggatccg gtcattttat ggggcgaagg
     6301 tttgcccgta gaacgtatcg ctgaaatgac aaaagtgagc gcttacgaac ttattacgcg
     6361 cctgacttca agggtcgcga tgaaatacgt ggattaatcg ttctgtaata tttgattgtc
     6421 tgtgccggat gcggcgtgaa tgccttatcc ggccaataaa atcctaaaaa ttcaataagt
     6481 tgatgttctt tcatgctctt ataaaggtcg tgcctctggc ggatgttcgt ttgtcatgag
     6541 tctcactctg ttgctaattg ccgttcgctc ctgaacatcc actcgatctt cgccttcttc
     6601 cggtttattg tgttttaacc acctgcccgt aaacctggag aaccatcgcg tgtttcaaaa
     6661 agttgacgcc tacgctggcg acccgattct tacgcttatg gagcgtttta aagaagaccc
     6721 tcgcagcgac aaagtgaatt taagtatcgg tctgtactac aacgaagacg gaattattcc
     6781 acaactgaaa gccgtggcgg aggcggaagc gcgcctgaat gcgcagcccc atggcgcttc
     6841 gctttattta ccgatggaag ggcttaacag ctatcgccat gccattgcgc cgctgctgtt
     6901 tggtgcggac catccggtac tgcaacaaca gcgcgtagca accattcaaa cccttggcgg
     6961 ctcaggggca ttgaaagtgg gcgcggattt cctgaaacgc tacttcccgg aatcaggcgt
     7021 ctgggtcagc gatcctacct gggaaaacca cgtagcaata ttcgccgggg ctggattcga
     7081 agtgagtact tacccctggt atgacgaagc gactaacagc gtgcgcttta atgacctgtt
     7141 ggcgacgctg aaaacattac ctgcccgcag tattgtgttg ctgcatccat gttgccacaa
     7201 cccaacgggt gccgatctca ctaatgatca gtgggatgcg gtgattgaaa ttctcaaagc
     7261 ccgcgagctt atcccattcc tcgatattgc ctatcaagga tttagtgccg gtatggaaga
     7321 ggatgcctac gctattcgcg ccattgccag cgctggatta cccgctctgg tgagcaattc
     7381 gttctcgaaa attttctccc tttacggcga gcgcgtcggc ggactttctg ttctgtgtga
     7441 agatgccgaa gccgctggcc gcgtactggg gcaattgaaa gcaacagttc gccgcaacta
     7501 ctccagcccg ccgaattttg gtgcgcaggt ggtggctgca gtgctgaatg acgaggcatt
     7561 gaaagccagc tggctggcgg aagtagaaga gatgcgtact cgcattctgg caatgcgtca
     7621 ggaattggtg aaggtattaa gcacagagat gccagaacgc aatttcgatt atctgcttaa
     7681 tcagcgcggc atgttcagtt ataccggttt aagtgccgct caggttgacc gactacgtga
     7741 agaatttggt gtctatctca tcgccagcgg tcgcatgtgt gtcgccgggt taaatacggc
     7801 aaatgtacaa cgtgtggcaa aggcgtttgc tgcggtgatg taatgcagga aagcaggctg
     7861 gagttaccca gcctgcagtg aaattaaact gtcgtcgctt tcactatttc tttgtagatg
     7921 atttttttga tgccgttgtt ctacgtgaga gataataaaa cgttgtcagt tcttttattg
     7981 ttaagtttat cccaattatc tggaattcct ttccctattt tttcgtgtgg agtaataatt
     8041 tctgcacatc taaccattat taaatttaat aataagtatc gggtttgtta cgatagaata
     8101 tttctatatc ctcaagaata tactttgcct tattgcctgt gactttctga gattcaatta
     8161 tagtatcgtt aaattctaat gttaaagaga actctttttt tccgctttgt gtaagtt
//
LOCUS       DAZPEP010000168         8193 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZPEP010000168 DAZPEP010000000
VERSION     DAZPEP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.169"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..496
                     /gene="radC"
                     /locus_tag="ACHUT9_003668"
     CDS             <1..496
                     /gene="radC"
                     /locus_tag="ACHUT9_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFR1350051.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            713..949
                     /gene="rpmB"
                     /locus_tag="ACHUT9_003669"
     CDS             713..949
                     /gene="rpmB"
                     /locus_tag="ACHUT9_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFR1350052.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            970..1137
                     /gene="rpmG"
                     /locus_tag="ACHUT9_003670"
     CDS             970..1137
                     /gene="rpmG"
                     /locus_tag="ACHUT9_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFR1350053.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1235..2044
                     /gene="mutM"
                     /locus_tag="ACHUT9_003671"
     CDS             1235..2044
                     /gene="mutM"
                     /locus_tag="ACHUT9_003671"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFR1350054.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHUT9_003672"
     CDS             complement(2083..2562)
                     /gene="coaD"
                     /locus_tag="ACHUT9_003672"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFR1350055.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHUT9_003673"
     CDS             complement(2570..3847)
                     /gene="waaA"
                     /locus_tag="ACHUT9_003673"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFR1350056.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHUT9_003674"
     CDS             4260..5318
                     /gene="rfaQ"
                     /locus_tag="ACHUT9_003674"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFR1350057.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5315..6439
                     /locus_tag="ACHUT9_003675"
     CDS             5315..6439
                     /locus_tag="ACHUT9_003675"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFR1350058.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHUT9_003676"
     CDS             6432..7229
                     /gene="rfaP"
                     /locus_tag="ACHUT9_003676"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFR1350059.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7245..>8193
                     /gene="waaO"
                     /locus_tag="ACHUT9_003677"
                     /gene_synonym="rfaI"
     CDS             7245..>8193
                     /gene="waaO"
                     /locus_tag="ACHUT9_003677"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008918 - lipopolysaccharide
                     3-alpha-galactosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HFR1350060.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFINAKEASPWKNEPFMRPINSNYARYCAKHNF"
BASE COUNT         2104 a         1887 c         2071 g         2131 t
ORIGIN      
        1 gttaacctct gaatatgaac aatttagtgg cgttcatgga attggcgtag cgaaatttgc
       61 ccagttaaag gggattgctg aactggcgcg gcgttactac aacgtgcgga tgcgtgaaga
      121 aagcccttta ctcagcccgg agatgacgcg ggaattttta caaagccagc tcacgggtga
      181 ggagcgggag atctttatgg tgatctttct cgactcacaa caccgggtta taacgcatag
      241 ccgtcttttt tccggcacgc taaaccatgt tgaagtccat cctcgggaaa ttatccggga
      301 agcgataaaa ataaacgcct cggcgctgat ccttgcacat aatcaccctt cgggttgtgc
      361 tgaacccagt aaagcggata aactcattac tgaacggata ataaagagtt gtcagttcat
      421 ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga gagtatgttt cttttgccga
      481 acgcggctgg atttaacccg ctatgcgcga tccttcggga tctttgtctg ttcgggactt
      541 gagcacatcg ctgagtcagc gtatactacg ccacctttga gaatctcggg tttggcattt
      601 gggcctggca atcgagagtt cacatagaac tgcgatgacc gggctgtaaa gcctgacgag
      661 gcgccaatac cccatacgaa gctcgagcta atttgatttt tggagaatag acatgtcccg
      721 agtctgccaa gttactggca agcgtccggt gaccggtaac aaccgttccc acgcactgaa
      781 cgcgactaaa cgccgtttcc tgccgaacct gcactctcac cgtttctggg ttgagagcga
      841 aaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg cgtgtaatcg ataaaaaagg
      901 catcgataca gttctggctg aactgcgtgc ccgtggcgaa aagtactaag tacttagagg
      961 aaataaatca tggctaaagg tattcgtgag aaaatcaagc tggtttcttc tgctggtact
     1021 ggtcacttct ataccactac gaagaacaaa cgtactaagc cggaaaaact ggaactgaaa
     1081 aaattcgatc cagttgttcg ccagcacgtg atctacaaag aagcgaaaat caaataattc
     1141 tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt tttgttatct gcttgccccc
     1201 atattgattg catctgttca ttcctggaga tgctatgcct gaattacccg aagttgaaac
     1261 cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc attcttcatg cggtggtgcg
     1321 caacggacgc ttgcgctggc cggtttcaga agagatctac cgtttaagcg accaaccagt
     1381 gcttagcgtg cagcggcggg ctaaatatct gctgctggag ctgcctgagg gctggattat
     1441 cattcatttg gggatgtctg gcagcctgcg catccttcca gaagaacttc cccctgaaaa
     1501 gcatgaccat gtggatttgg tgatgagcaa cggcaaagtg ctgcgctaca ccgatccgcg
     1561 ccgctttggt gcctggctgt ggaccaaaga gctggaaggg cacaacgtgc tggctcatct
     1621 tggaccggag ccgcttagcg acgatttcaa tggtgagtat ctgcatcaga agtgcgcgaa
     1681 gaaaaaaacg gcgattaaac cgtggctgat ggataacaag ctggtggtag gggtagggaa
     1741 tatctatgcc agcgaatcac tgtttgcggc ggggatccat ccggatcggc tggcgtcatc
     1801 actgtcgctg gcagagtgtg aattgttagc tcgggtgatt aaagcggtgt tgctgcgttc
     1861 gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa agtgatggta aaccgggcta
     1921 tttcgcgcag gaattgcagg tttacgggcg aaaaggtgag ccgtgtcggg tgtgcggtac
     1981 gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt tattgtcggc agtgccagaa
     2041 gtaattcata cgcgccggat ggcataccat ccggcataaa cgctacgcta acttcgccac
     2101 cagcgcctga tggacattct ccggcaggaa atgggtgaca tcgccctgat ggcgcgccac
     2161 ctctttcacc aacgatgaag agataaacga ccactctttc gacggcatca gaaacacact
     2221 ttccagttcc ggcattaagt ggcgattcat atgcgccagc tgcatttcat attcaaaatc
     2281 cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt tgattacggg cgaagttcgc
     2341 catcaaatca ctaaacccaa ccacttccac gttccccaga tgcgcggttg cctgctgtgt
     2401 cagcgccaca cgctcttcca gggtaaacat cggtttttta ctggggctgg cggcaatcgc
     2461 cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg atatcgatat gaccattggt
     2521 aatgggatcg aaagtacccg gataaatcgc ccgtttttgc ataacaacct caatgcgttt
     2581 tcggtggcag gtaaggttcc agcagttgaa gcagacgctg tagcgcgccc tggttttgat
     2641 acagtacttc aacggcatga cggccataga aactacggta atcggcgtcg gtgagtaaag
     2701 aggaaacctc ttttgcaagc gtagtggcat cggtaacggt aatcagcccg cttgcctgct
     2761 ccagccgcgc gcaaatgtct ttgaaattaa aagtatgcgg ccccatcaat accggaatag
     2821 cgtgtgcggc agcttccaga ggattatgcc ctccacgttc aaccagtgaa ccgccaacaa
     2881 aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc catcgtatcg ccaaccacaa
     2941 cctgcgtgct ggtggagggg acttcccctg aagagcgtgt gatatagctt agtccagcct
     3001 ggcggacaag gttaatcgca tccgggaagc gttctggatg acggggtacc aggatgagca
     3061 ataaattcgg gaattgctgt aataatgcct gatgtgcggc gatcaccaca ctctcttcac
     3121 cttcgtgagt gctggtggca atccataccg ggcggtgtgg tgcccactgg cggcgcagcg
     3181 tcacggcttt agcagccaac tgcggcgtta cagaaatatc gaatttcagg ctaccggtaa
     3241 cggttacctg gttatttttt gcgcccagcg ccacaaaacg tgcaccatct tcttcatttt
     3301 gcgcagcaat cagcgtaata cgacgcagta agcggcggac gaatttaccc agtttggcat
     3361 aacctgcggc cgagcgggca gagagtcgcg cgttagcgat caccagcgga attttacgtt
     3421 tatgtagcgc cgcaatcagg ttaggccata gttcggtttc cataatcaac accagtttgg
     3481 ggtcgacttt attcaggaaa cggttgagcg catcgggcag gtcatacggc agataaacgt
     3541 gctgaacatc cttcccgaaa gccgattgta cgcgctccga accggttggc gtcatggttg
     3601 taacggtaat cggtaaatca ggataacgat ggcgcagcgc gcgcaccaac gggatcgccg
     3661 ccagagtttc accgacggag acggagtgca gcataatgcc gcctggtttt agcggatggc
     3721 ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc cggagcctta cgtccgcgca
     3781 cccagagccg tatccagatc agcggctgaa taaggtagag aagggcggtg taaagcaatt
     3841 cgagcatagt aaatagctga cttatggatg tgctggggat tctatgtatt tagctgtggc
     3901 tttaccatta cttttcccgt ttttgactta aatagcaatt tacccgccaa ttcttgctga
     3961 acatctatac tttttttgta tttttatgcg attcattgat ttctgctgta cgtgcaggaa
     4021 ggaatcgcag agcaacagtg aaagaatatg gttgctaatg attttatttg attgatttct
     4081 ttattgaaaa tcaacaatgt gcttaaaaag cattcgggtc ggattgccgc gaaagaagtg
     4141 atacactagc gcgtcaatat cagtgcggta tcagtattca ggtagctgtt gagcctgggg
     4201 cggtagcgtg ctttttctgc ttcaccttaa tcggataatc tcaacaaaaa gagttacttg
     4261 tggataagcc atttcgaaga attttgctca ttaagatgcg ttttcatggg gatatgttat
     4321 taactactcc cgtcattagt tcgctgaaaa aaaattaccc tgacgcaaaa atcgatgtgc
     4381 tgctttatca ggacaccacc ccgatcctgt ctgaaaatcc agagattaac gcgctctacg
     4441 gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc caactttttt catctcatca
     4501 aggtattacg tgccaataag tatgacctta tcgtcaatct taccgatcaa tggatggttg
     4561 ctatactggt tcgcttatta aatgcccgtg tgaaaatttc ccaggattat catcatcggc
     4621 agtctgcttt ttggcgtaaa agtttcaccc atttagtgcc gttgcagggt ggaaatgtgg
     4681 tggaaagtaa cttatccgtg ctgacaccat tgggacttga ttcgttggtg aagcagacaa
     4741 ccatgagtta cccgcctgca agctggaaac gtatgcgtcg cgaacttgat cacgctggtg
     4801 ttggacaaaa ttatgtggtt atccaaccta cggcgcggca aatcttcaaa tgctgggaca
     4861 acgccaagtt ttccgctgtg attgatgcct tacatgctcg tggttatgaa gtcgttctga
     4921 cgtccggccc agataaagac gatctggcct gcgtcaatga aattgcgcag ggatgccaga
     4981 cgccaccagt aacggcgctg gctggaaagg tgaccttccc ggaacttggt gcgttaatcg
     5041 atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc gcatattgcc gctgcagtta
     5101 atacgccgct gatatcgctg tttggtgcga cagaccatat tttctggcgt ccttggtcaa
     5161 ataacatgat tcaattctgg gcgggagatt accgggaaat gccaacgcgc gatcagcgtg
     5221 accgaaatga gatgtatctt tcggttattc cggcggcaga tgtcattgct gctgtcgata
     5281 aattactgcc ctcctccacg acaggtacgt cgttatgatc gttgcttttt gtttatataa
     5341 atattttccc tttggcggtt tgcagcgcga ttttatgcgt attgctcaga cagtcgccgc
     5401 ccgaggtcat catgttcggg tttataccca gtcgtgggaa ggcgaatgcc ctgatgtatt
     5461 tgaactgatc aaagtgccgg ttaaatcgca taccaatcac gggcgcaatg cggagtattt
     5521 tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat aaagtcgttg gattcaacaa
     5581 aatgccgggg ctggacgttt attatgccgc tgatgtttgt tatgccgaga aagtcgcgca
     5641 ggaaaaaggc tttttctatc acctgacgtc acgttatcgc cattatgccg cctttgagcg
     5701 ggcaaccttc gaacagggca agccgacaca gctgctgatg ctgacagata agcaaatcgc
     5761 cgatttccag aaacattatc agactgaagc ggagcgtttt catattctgc caccggggat
     5821 ttatcctgat cgtaaatata gccagcagcc agctaatagc cgtgaaatct tccgtaagaa
     5881 gaatggaata accgaacaac aatatttatt gttgcaggtc ggttcagact tcacgcgtaa
     5941 aggtgtcgat cgttccattg aagcacttgc ttcgttaccg gattcgctgc gccacaacac
     6001 attgctatat gttgttggac aggataagcc gcgaaaattt gaggcgctgg cagaaaaacg
     6061 cggcgtgcgc agtaatgttc acttcttctc ggggcgcaac gatgtctcgg aattaatggc
     6121 ggcggcggat ttattactgc atcctgccta ccaggaagca gcgggaattg tgctactgga
     6181 agcgattact gcaggattac cggtactaac aacagccgtt tgtggctatg cgcattatat
     6241 tgtcgacgcc aattgcggcg aggctattgc tgagccattc cgccaggaaa cattgaatga
     6301 gattttacgc aaagcgttaa cgcaatcttc attgcgccag gcctgggcgg aaaatgcgcg
     6361 acattatgct gatacacaag atttatacag tctgccagag aaagcggcgg atatcataac
     6421 gggtggtctg gatggttgaa cttaaagagc cgtttgccac gttatggcgc ggtaaagatc
     6481 cttttgagga agttaaaacc ttgcagggtg aggtttttcg tgaactggaa actcgccgca
     6541 ctctgcgatt tgaaatggcg ggcaaaagct attttctcaa atggcatcgc ggcacgaccc
     6601 tgaaagagat aatcaaaaat ttactctcat tgcggatgcc agtattaggc gcagaccgcg
     6661 aatggaatgc gattcatcga ctgcgggatg tcggcgttga tactatgtat ggggtggcat
     6721 ttggcgaaaa aggtattaat ccgctgagca gaacctcgtt tattattacc gaagatctga
     6781 ccccaaccat cagtctggaa gattactgtg ctgactgggc gaccaaccca ccagatgttc
     6841 gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt gcgcgatatg catgctgtgg
     6901 gcattaacca ccgtgactgt tatatctgtc atttcctgct gcacttgcct ttttccggta
     6961 aggaagagga gttaaaaatt tcggtaattg acctgcaccg ggcgcagctt cgcacgcgcg
     7021 ttccacgtcg ttggcgcgat aaagatctta ttgggcttta tttttcttcg atgaatatcg
     7081 gcctgactca gcgggatatc tggcggttta tgaaagtgta ttttgtcgcc ccgcttaaag
     7141 acattctcaa gcaggaacaa ggactgctgt cgcaagcaga agcaaaagcc acaaaaatca
     7201 gggaaagaac gattcgaaaa tcgttgtaat ttccggagag aaatatgagt gcccactatt
     7261 ttaatccaca agagatgatc aataagacaa tcatcttcga tgaaaggcca gcggcgtcag
     7321 tggcatcatc attccatgtt gcttatggca ttgataaaaa ctttcttttt ggttgtggtg
     7381 tttcaatcac gtcagttttg ttacataaca acgacgtgag ttttgttttc cacgttttta
     7441 ttgatgatat ccctgaagcc gatatccagc gtttagccca attggcgaaa agctatcgta
     7501 cctgtatcca gatccatctg gtaaattgtg aacggcttaa ggcattaccg acgaccaaaa
     7561 attggtctat tgccatgtat ttccgttttg taattgcaga ttactttatt gatcaacaag
     7621 ataagatcct ttacctggat gctgatatcg cctgtcaggg aaacttaaag ccgctgataa
     7681 caatggatct tgccaataac gttgctgctg ttgttactga acgcgatgct aactggtggt
     7741 cgttacgggg tcaaagtctg cagtgtaatg aacttgaaaa gggttacttt aattcaggtg
     7801 tcctgttaat taatacacta gcgtgggcgc aggagtccgt ttctgctaaa gcgatgtcga
     7861 tgcttgctga taaagccgtc gtttcccgtt taacctatat ggatcaagat atacttaatc
     7921 ttatcctgtc agggaaagtt aaattcattg atgctaaata caatactcaa tttagtttaa
     7981 attatgaatt aaaaaaatca tttgtttgtc caattaatga tgaaaccgta ttaattcatt
     8041 atgtcggccc gacaaaaccc tggcattact gggccggtta tccaagtgcg caacctttta
     8101 tcaacgccaa agaagcatcg ccctggaaaa atgaaccgtt tatgcggcca attaattcaa
     8161 actatgctcg ttattgcgcc aagcataatt tta
//
LOCUS       DAZPEP010000169         8184 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZPEP010000169 DAZPEP010000000
VERSION     DAZPEP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.170"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..103
                     /gene="mgtT"
                     /locus_tag="ACHUT9_003678"
     CDS             <1..103
                     /gene="mgtT"
                     /locus_tag="ACHUT9_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFR1350061.1"
                     /translation="NGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHUT9_003679"
     CDS             complement(218..1108)
                     /gene="dgcZ"
                     /locus_tag="ACHUT9_003679"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFR1350062.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHUT9_003680"
     CDS             complement(1363..1755)
                     /gene="ydeI"
                     /locus_tag="ACHUT9_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFR1350063.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2031..2549
                     /locus_tag="ACHUT9_003681"
     CDS             2031..2549
                     /locus_tag="ACHUT9_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFR1350064.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="ACHUT9_003682"
     CDS             complement(2594..4639)
                     /gene="dcp"
                     /locus_tag="ACHUT9_003682"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFR1350065.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4776..5522
                     /gene="ydfG"
                     /locus_tag="ACHUT9_003683"
     CDS             4776..5522
                     /gene="ydfG"
                     /locus_tag="ACHUT9_003683"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFR1350066.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5611..6297
                     /gene="rspR"
                     /locus_tag="ACHUT9_003684"
                     /gene_synonym="ydfH"
     CDS             5611..6297
                     /gene="rspR"
                     /locus_tag="ACHUT9_003684"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFR1350067.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6474..6677
                     /gene="ydfZ"
                     /locus_tag="ACHUT9_003685"
     CDS             6474..6677
                     /gene="ydfZ"
                     /locus_tag="ACHUT9_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFR1350068.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6713..8173)
                     /locus_tag="ACHUT9_003686"
     CDS             complement(6713..8173)
                     /locus_tag="ACHUT9_003686"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFR1350069.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2160 a         2053 c         1866 g         2105 t
ORIGIN      
        1 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
       61 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
      121 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
      181 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
      241 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
      301 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
      361 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
      421 aaattctgac acctgcacga catgcttctt catcattagc cgctttaaca ataatgataa
      481 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
      541 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
      601 taaatcggtc aatatccaac aacattaaat aaagattcag aggatcagtg ttgcgtaact
      661 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
      721 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
      781 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
      841 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
      901 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
      961 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     1021 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     1081 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     1141 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     1201 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     1261 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     1321 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     1381 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     1441 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacaacat taatttcacc
     1501 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     1561 tgaaaccgaa gcgccatcgt gcatgttttt tgcgaaatca acggtcattt tttttgcatc
     1621 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     1681 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     1741 ggactgaaat ttcatactaa ctccttaatt gcgtttggtt tgacttatta agtctggttg
     1801 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagc
     1861 ccactagctg tcaaatattc agagaatttg actcactaaa gttaagaaga ttgaagagtc
     1921 ttaaacatgt tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc
     1981 atacagaaaa agcaaatcac ctttacatat aatagcgtgg acaaaaaaca gtgaacatta
     2041 ataaagataa aattgtacaa ctcgcagata ccgacactat tgaaaacctg acatccgcgt
     2101 tgagtcaaag acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg
     2161 gtaagttggc tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct
     2221 ttgttacttt caccgatcag gcaaagatga aaatcctcag cgtaagccag cagtctcttg
     2281 aacgatattc tgcggtgagt gagaaagtgg cagcagaaat ggcaacaggt gccatagagc
     2341 gtgcggatgc tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag
     2401 atggtacgcc agcgggtacc gtctggttcg cgtggcatat taaaggccag aactacactg
     2461 cggttatgca ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc
     2521 tcgcccagct gctgcaatta ctgctataac caggctggcc tggcgatatc cccaggccag
     2581 ccattggtgg tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag
     2641 ccttaccgcg ccattgtcga tgcaggcgtt ccagatcttc gctgttacct ctggaaagga
     2701 tcgcctcgcg aaaacgctgc ccattttcac gcgttaatcc gccctgctca acaaaccact
     2761 ggtaaccatc atcggccaac atttgcgtcc acagataagc gtaataacct gcagcatatc
     2821 cgccaccaaa aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat
     2881 ccatattttc cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt
     2941 tttcttccag gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct
     3001 catacccttt gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg
     3061 ctgccccgct ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt
     3121 gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg
     3181 tggcataacg ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg
     3241 ttattacatc atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc
     3301 agacgttata aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca
     3361 tccatgcacc gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagcc
     3421 ccacgccatt atgatcaaaa atttcccaca cccgaacgtc aggatggtag acaggaatat
     3481 caaaacgttc gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat
     3541 tcaacaccgt gtttaattca aaatatggct tgagctgcgc ttcatcaaga tcaaatttct
     3601 cccgccgtac ctgttccgca taaaatgccc agtcccacgg ctgcgcgcta aacccgccct
     3661 gctgcttatc gataaccgcc tgtatggagg ctaattcatc gctcgcacgt tgacgcgccg
     3721 ctggaacaat ttcccgcata aagttgagtg ctgcttctgg cgtttttgcc atctgatcgg
     3781 cgattttcca tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct
     3841 ctaccagacg ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc
     3901 agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt
     3961 gttgggtggt attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg
     4021 cctctgccgc cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca
     4081 caaccagacc gccggattta tttgctgcca gtaatcgctg gttaaactgg ctggtcaggg
     4141 tcgcagcttc tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc
     4201 cggcaaggac aaaacgttga tgaatcacct ccaccaagcg gatggattca ctatcaagcc
     4261 ccagggattc acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat
     4321 agatatcatt agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt
     4381 catcattggt atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt
     4441 ctccgctttg ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg
     4501 cgatggcagc aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt
     4561 gatgattggc aatttgatca aaatggggag ccagatacgg tagtgtgctt tgcacaagga
     4621 aaggattcat tgttgtcatt ttcttctcct gaacgcgaga tgttccatag cgtaggctta
     4681 ctgataacgg agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat
     4741 gttattgaca cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa
     4801 cggcaggttt tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg
     4861 ccactggccg tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt
     4921 atatcgccca actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc
     4981 ctgccgagtg gtgcaatatt gatatcctgg taaataatgc cggcctggcg ttgggcatgg
     5041 agcctgcgca taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag
     5101 gcctggtata tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata
     5161 ttattaacat tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg
     5221 cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg tacggacctg catggtacgg
     5281 cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc
     5341 gctttaaagg cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc
     5401 cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca
     5461 ataccctgga aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt
     5521 aatttttata cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca
     5581 tgcagttaac tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca
     5641 cagcctgtca atcagcaaat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt
     5701 gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag
     5761 ccggttcgtg aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag
     5821 cgtggcagct acgtcaacaa aatttccatg gcccaggtgc gcaatggcag ttttatccgt
     5881 caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc
     5941 taccaactgg aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat
     6001 gatttttttg aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa
     6061 ctggcgtggg ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt
     6121 ttcgaccacg tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc
     6181 ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc
     6241 agcgaatccg tgcgccagat ccgccaggaa aatagcgact ggtttagcga agagtaattc
     6301 atttcctctc atcccatccg gggtgagagt cttttccccc gccttatggc tcatgcatgt
     6361 atcaaaaaag atgtgagctt gatcaaaaac aaaaaatatt tcactcgaca ggagtattta
     6421 tattgcgccc gttacgtggg cttcgactgt aaatcagaaa ggagaaaata cctatgacga
     6481 cctacgatcg taaccgtaac gcaatcacca ctggcagccg tgttatggtt agcggcaccg
     6541 gtcacactgg caagatcctg tcgattgata ctgaaggtct gaccgctgag caaatccgcc
     6601 gcggaaaaac cgtagttgtt gaaggttgtg aagagaaact ggcacctctg gacctgattc
     6661 gtctcggcat gaactaagcg tgtgaatgcc gccgatggcg gcattgcttt ttttacttca
     6721 cggaatattt cgccacggtc gctttcgcgc catgcgctaa taaagacaag tacgcttccg
     6781 tcacttttgc agtaaacaaa ctattgtctg gcaaatcatc accaaagatc gccttaatcg
     6841 ccagcaatga ctggacgcgc gctttccctt cagcactact ttgtacagcc ttctgaataa
     6901 caggtaacag tgggtcactg atttcaatcg gatttccctg ttcatcaaca ccaccgacat
     6961 aacgcatcca acccgcgacg cccagtgcca gcagatcgaa cttgctgtca tgcgccagat
     7021 gccagcgaac agaatccaac atccgctgtg gcaatttctg gctgccatcc atcgcaatct
     7081 gccaggttcg atgacgtaac gccgggttgc tatagcgttc aattaatcgg ttagcgtaat
     7141 cttgcaaatc aacgccctgc actttcagcg tcggcgcttg ttcctgcaac atcaagccat
     7201 acgccgcata acgataatgt tcatcttcca tgcagtcatt aatgtgctga tatccagcaa
     7261 gataacccaa atacgccagg aatgaatgac tgccgttgag catgcgcaac ttcatctctt
     7321 cataaggcag cacatcacta accagttcgg ctcccgcttt ttcccattct ggacgtccgg
     7381 caacaaagtt atcttctatt acccactggc ggaaaggttc acaggcaacg cccgcaggat
     7441 cgcccacacc ggtaagttgt tcgattttcg ccagcgtatc ctctgtcact gcgggcacaa
     7501 tacggtccac cattgttgat gggaaagtca cgttatcttc gatccattgt gccagtttta
     7561 catcaacagc tttcgcgtat gaagtgacaa cgtcacgcat aacatgaccg ttttctggca
     7621 tgttgtcaca tgacatgacg gtaaatgcgg gaagtcctgc cgctttacgg cgggccagcg
     7681 cctcaacaat cacccctgtt gctgttttcg gctggtgggg attttgcacg tcggcagcga
     7741 ccatcgggtg atcgagcatt aactgtccgg tcgccggaga gtggaaatac cctttttcgg
     7801 tgattgtcag agagacaatc gcgatttgcg gttcacacat cgcagccaac acggtttcta
     7861 agccatccat ctgtacgtgc aaggctttct taacgacgcc aacgacgcga gccgtccacg
     7921 catcggccga catttccgca acggtataaa gattatcttg ctgttgtaaa tcggcaattt
     7981 gctgttcgcc gccgattaag ttgacctcat aatatcccca gtcactgaaa tgttctgtag
     8041 caagaatatc ggcatacacg ccctgatgcg cacggtgaaa tgcaccaaag cctaaatgaa
     8101 taattcttgg ggccaggtta ttacgatcat aaacagggag tgtcgctttt gctgataaca
     8161 aattatttcc cataacaatt cctt
//
LOCUS       DAZPEP010000170         8182 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZPEP010000170 DAZPEP010000000
VERSION     DAZPEP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8182
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.171"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUT9_003687"
     CDS             <1..2847
                     /gene="yfaL"
                     /locus_tag="ACHUT9_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFR1350070.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUT9_003688"
     CDS             complement(2975..3697)
                     /gene="ubiG"
                     /locus_tag="ACHUT9_003688"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFR1350071.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUT9_003689"
     CDS             3844..6471
                     /gene="gyrA"
                     /locus_tag="ACHUT9_003689"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFR1350072.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6679..>8182
                     /locus_tag="ACHUT9_003690"
     CDS             6679..>8182
                     /locus_tag="ACHUT9_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFR1350073.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2031 c         2321 g         1851 t
ORIGIN      
        1 gccgacggaa aaacgctggt tattggcaat acagagaatg acggagctgt tgactctatt
       61 gctggtaccg ggttgatcac caaaacaggt tccggcgatc tggtacttaa tgcagataac
      121 aatgacttta ctggtgagat gcagattgaa aacggtgaag ttaccctggg ccgcagcaac
      181 tccctgatga atgtcggcga tacgcattgc caggacgatc cgcaagactg ctacggtctg
      241 acgataggga gtattgataa gtaccagaat caggcagagc taaacgttgg ctccacccaa
      301 caaacctttg tgcactcatt gacgggcttt cagaatggca ctttaaatat cgatgctggt
      361 ggcaatgtta ctgttaatca aggcagtttt gctggcacca tcgaaggtgc tggtcagctc
      421 accattgcgc aaaacggcag ctacgtgctg tcgggggcgc agtcgatggc gctaaccggc
      481 gatatagtcg ttgatgatgg tgcggtgctt tcgctggaag gcgacgcggc agatctcgcc
      541 gctctccagg acgatccgca gtcgatcgtg ttaaacggcg gtgtgctcga tctctctgat
      601 ttctccacct ggcagagcgg cacatcatac aatgatggcc ttgaagtcag tggcagcagc
      661 ggaacggtta tcggcagtca ggatgtggta gatcttgcag gtggcgacaa tttgcatatc
      721 ggcggcgacg ggaaagatgg cgtctacgtg gtggtcgatg cgagcgacgg gcaggtaagt
      781 ctggcaaaca ataatagtta tttgggcaca acacaaatcg cctccggtac gctgatggtg
      841 agcgacaact cgcagcttgg agatacccac tataaccgcc aggttatctt taccgataag
      901 caacaagaaa gcgtgatgga gattactgcc aatgtcgata ctcgctctac gacgactgag
      961 catgggcgtg atattgaaat gcgcgccgac ggtgaagtgg cagttgatgc gggggtagac
     1021 acgcagtggg gcgcactgat ggctgacagc agcgggcagc atcaggatga gggtagcaca
     1081 tttactaaaa cgggggcggg tacgctggag ctgaccgcca gcggtacaac gcagtcggca
     1141 gtacgtgtcg aagaaggcac cctgaaaggc gatgttgcgg atatctttcc ttatgcttcg
     1201 tcactgtggg tcggtgatgg ggcggcgttc gttactggcg cggatcagga tattcagtct
     1261 atcgatgcta cttccagcgg cactatcgat atcagcgatg gtacggtttt gcgtctgacc
     1321 gggcaggata cttccgtcgc ccttaatgcc tcattgttta acggcgatgg gacgctggtg
     1381 aatgctaccg atggcgtgac gttgacaggt gagcttaata ccaaccttga aactgacagc
     1441 ctgacctatc ttgccgacgt gacggttaat ggtgatctga ccaatacgtc cggtgcggtt
     1501 agcctgcaaa atggcgtcgc tggcgatacg ctgacggtaa acggtgatta taccggcggc
     1561 ggtacgctac tgctcgatag cgaattaaac ggcgatgact cggtaagcga tcaattggtg
     1621 atgaacggta atactgctgg caataccacg gtggtggtta actctattac agggattggt
     1681 gagccgacat cgacaggcat taaagtggtt gatttcgcag ctgatcccac gcagtttcaa
     1741 aacaatgcgc agttcagtct ggcaggcagc ggctacgtca atatgggagc gtatgactac
     1801 acgctggtgg aagataacaa cgactggtat ctgcgatcgc aagaagtaac gccgccatcg
     1861 ccacctaatc ccaacccgac tcccgatcct gatcccacac cggatcctga cccaaccccc
     1921 gacccggccc ctacgcctgc ttaccagccg gtgctgaatg ccaaagttgg cggttatctc
     1981 aataacctgc gggcggcaaa tcaggcgttt atgatggagc gacgcgatca cgctggtggc
     2041 gatggtcaga cgctgaattt acgtgttatc ggcggacgtt atcattacac ggcggcaggg
     2101 caactggctc agcaggaaga cacttctacg gtgcagctta gcggcggctt gtttagcggg
     2161 cgctggggca cggatggcga gtggatgctt ggggcggttg gtagctacag tgataatcag
     2221 ggcgacagcc gctcgaatat gaccggaact cgcgccgata accagaacca cggttatgcc
     2281 gttgggctga cctcaagctg gtatcagcac ggtaatcaga agcagggggc ctggctggat
     2341 aactggttgc agtacgcgtg gtttagcaat gatgtttctg aacatgaaga tggcacagat
     2401 cattaccatt cgtcggggat tatcgcctcg ctggaagccg ggtatcagtg gttaccgggg
     2461 cgtggtgtgg tgattgaacc gcaggcgcag gtgatttatc agggcgtgca gcaggatgat
     2521 tttaccgccg ctaaccatgc gcgcgtgtca caatcgcagg gggatgatat tcagacgcgg
     2581 ctgggtttac acagcgaatg gcgtaccgcg gttggtgtca caccaacatt agatctgaat
     2641 tattatcacg atccccatgc gacggaaatt gaagaagatg gcagcactat cagtgacgat
     2701 gcggtgaagc gacggggtga aataaaagtg ggagtcacgg gcaatatcag tcagcgagtg
     2761 tccctgcgcg gcagcgtggc gtggcagaaa gggagtgatg attttgccca gacggcaggg
     2821 tttttgtcga tgacggtgaa atggtaatcg ctgtctgacc atcacaattg ccggatgcgg
     2881 cgtaaacgcc ttatctggcc tacggagagc atttgtaggc ctgataagac gctgcaagcg
     2941 tcgcatcagg cattggcgcg gccaaacatc aacctcactt attctgcgtg tgcagcatat
     3001 agttcacatc cacgccgggg ccgagcttaa aggaattagt gatcgggttg taatgtagcc
     3061 cggttatatg tcgctctttt aaactggtct gatccaccca gccaagcagc tctgccggtt
     3121 taataaactt cttcacatca tgcgtacctt tgggcaccat gcgcaaaata tattccgcgc
     3181 caaccaccgc catcagccat gacttgccgt tgcggttaag cgtcgagaaa aagacatcgc
     3241 cgcctggttt caccagttgc gcacaggctc tgaccactga ctgcggatcg ggtacgtgct
     3301 ccagcatctc catgcaggtc accacatcat actgcccggc atgttttgcc gcgtgctctt
     3361 ccacggtttc ctgcacgtaa tccacctgaa tgccgctttc cagcgcgtgc agctttgcca
     3421 cctgcaatgg ctcaaagccc atatccagac cggtcaccgt cgcgccttcg cgcgccatac
     3481 tctcggccag aatgccgccg ccacaaccga catcgagcac ctttttgcca aataaaccgc
     3541 cagcacgctc ggcaatatag cccagacgca gcgggttaat gcggtgcaac ggtttgaact
     3601 caccttcaag atcccaccag cgggaggcga cggcttcaaa tttagcgatc tcttcgtggt
     3661 ctacgttatg gttttccggc gatttttcgg cattcattgg cacttctact ccgtaattgg
     3721 caagacaaac gagtatatca ggcattggat gtgaataaag cgtataggtt tacctcaaac
     3781 tgcgcggctg tgttataatt tgcgaccttt gaatccggga tacagtagag ggatagcggt
     3841 tagatgagcg accttgcgag agaaattaca ccggtcaaca ttgaggaaga gctgaagagc
     3901 tcctatctgg attatgcgat gtcggtcatt gttggccgtg cgctgccgga tgtccgagat
     3961 ggcctgaagc cggtacaccg tcgcgtactt tacgccatga acgtactagg caatgactgg
     4021 aacaaagcct ataaaaaatc tgcccgtgtc gttggtgacg taatcggtaa ataccatccc
     4081 catggtgact cggcggttta tgacacgatc gtccgtatgg cgcagccatt ctcgctgcgt
     4141 tacatgctgg tagacggtca gggtaacttc ggttccatcg acggcgactc tgcggcggca
     4201 atgcgttata cggaaatccg tctggcgaaa attgcccatg aactgatggc cgatctcgaa
     4261 aaagagacgg tcgatttcgt tgataactat gacggcacgg aaaaaattcc ggacgtcatg
     4321 ccaaccaaaa ttcctaacct gctggtgaac ggttcttccg gtatcgccgt aggtatggca
     4381 accaacatcc cgccgcacaa cctgacggaa gtcatcaacg gttgtctggc gtatatcgat
     4441 gatgaagaca tcagcattga agggctgatg gaacacatcc cggggccgga cttcccgacg
     4501 gcggcaatca ttaacggtcg tcgcggtatt gaagaagctt accgtaccgg tcgtggcaag
     4561 gtgtacatcc gcgcccgtgc tgaagtggaa gtcgacgcca aaaccgggcg tgaaaccatt
     4621 atcgtccacg aaattccgta tcaggtaaac aaagcgcgcc tgatcgagaa gattgcggaa
     4681 ctggtaaaag aaaaacgcgt ggaaggcatc agcgcgctgc gtgacgagtc tgacaaagac
     4741 ggtatgcgca tcgtgattga agttaaacgc gatgcggtcg gtgaagttgt gctcaacaac
     4801 ctctactccc agacccagtt gcaggtttct ttcggtatca acatggtggc attgcaccat
     4861 ggtcagccga agatcatgaa cctgaaagat atcatcgcgg cgtttgttcg tcaccgccgt
     4921 gaagtggtga cccgtcgtac tattttcgaa ctgcgtaaag ctcgcgatcg tgctcatatc
     4981 cttgaagcac tagccgtggc gctggcgaac atcgacccga tcatcgaact gatccgtcat
     5041 gcgccgacgc ctgcagaagc gaaaactgcg ctggtggcta atccgtggca gctgggcaac
     5101 gttgccgcga tgctggaacg tgctggcgac gatgctgcgc gtccggaatg gctggagcca
     5161 gagttcggcg tgcgtgatgg tctgtactac ctgaccgaac agcaagctca ggcgattctg
     5221 gatctgcgtt tgcagaaact gaccggcctt gagcacgaaa aactgctcga cgaatacaaa
     5281 gagctgctgg atcagatcgc ggaactgttg cgtattcttg gtagcgccga tcgtctgatg
     5341 gaagtgatcc gtgaagagct ggagctggtt cgtgaacagt tcggtgacaa acgtcgtact
     5401 gaaatcaccg ccaacagcgc agacatcaac ctggaagatc tgatcaccca ggaagatgtg
     5461 gtcgtgacgc tctctcacca gggctatgtt aaatatcagc cgctttctga atacgaagcg
     5521 cagcgtcgtg gcgggaaagg taaatctgcc gcacgtatta aagaagaaga ctttatcgac
     5581 cgactgctgg tggcaaacac gcacgaccat attctgtgct tctccagccg tggtcgcgtc
     5641 tattcgatga aagtttacca gttgccggag gccactcgtg gcgcgcgcgg tcgtccgatc
     5701 gtcaacctgc tgccgctgga gcaggacgaa cgtatcaccg cgatcctgcc agtgaccgag
     5761 tttgaagaag gcgtgaaagt cttcatggcg accgctaacg gtactgtgaa gaaaaccgtc
     5821 ctcaccgaat tcaaccgtct gcgtaccgcc ggtaaagtgg cgatcaaact ggttgaaggc
     5881 gatgagctga tcggcgttga cctgaccagc ggcgaagacg aagtaatgct gttctccgcc
     5941 gaaggtaaag tggtgcgctt taaagagtct tctgtccgtg cgatgggctg caacaccacc
     6001 ggtgttcgcg gtattcgctt aggtgaaggc gataaagtcg tctctctgat cgtgcctcgt
     6061 ggcgatggcg caatcctcac cgcaacgcaa aacggttacg gtaaacgtac cgcagtggcg
     6121 gaatacccaa ccaagtcgcg tgcgacgaaa ggggttatct ccatcaaggt taccgaacgt
     6181 aacggtttag tggttggcgc ggtacaggta gatgactgcg accagatcat gatgatcacc
     6241 gatgccggta cgctggtacg tactcgcgtt tcagaaatca gcatcgtggg ccgtaacacc
     6301 cagggcgtga tcctcatccg tactgcggaa gatgaaaacg tagtgggtct gcaacgtgtt
     6361 gctgaaccgg ttgacgagga agatctcgat accatcgacg gcagtgccgc ggaaggggac
     6421 gatgaaatcg ctccggaagt ggacgttgac gacgagccag aagaagaata attttacttc
     6481 ttcatgccaa aagggagcta tctcccttgt ttgaattgaa aagtccaggc tgcaaagtct
     6541 gggcttttgt cgtattaggg cacggtaaag tttggctgtg cccgtaaaaa atggctggct
     6601 atacacaagg aatgtggcaa tgagtggtga aaaaaagcga aaggctggcg gttctatggt
     6661 cttgtaggtt ttggcgcaat agtactgctt tccgctggtg tctgggcgtt gcaatatgct
     6721 ggcagtgggc cagaaaaaac gttgtcgccg ctggtggtgc acaacaatct gcaaatcgat
     6781 ctcaatgagc cggacctctt tctcgacagc gactctctga gccagcttcc caaagatctc
     6841 ctcaccattc cgtttctcca cgatgttctg agtgaagatt tcgttttcta ttatcagaat
     6901 catgccgatc gtctgggcat tgaaggcagc attcgtcgca ttgtctatga acacgatctc
     6961 acgctgaaag ataaactctt ttcgtcactc ttagatcagc ccgcgcaggc tgcgctatgg
     7021 cacgataaac aaggccatct ttcacattac atggtgctga tccagcgcag tggtttaagc
     7081 aaactgctgg agccattgtt gtttgccgct accagcgaca gccagttaag caaaacggaa
     7141 atcagtagca tcaagataaa tagtgaaact attcctgttt atcagttgcg ctataacggc
     7201 aataacgccc tgatgttcgc gacttatcag gacaagatgc tggtgttttc cagcacggat
     7261 atgttgttta aagatgatca gcaggatacc gaagccacgg cgatcgcagg tgatttgttg
     7321 agcggcaaaa aacgctggca agcaagcttt ggcctggaag agcgtgctgc tgaaaaaacg
     7381 ccagtacgcc agcgcatcgt ggtcagcgcc aggttgctgg gttttggcta ccagcggtta
     7441 atgccttctt ttgctggcgt acgcttcgaa atgggtaacg acggctggca cagttttgtg
     7501 gcgttaaatg atgaatccgc cagcgtagat accagtttcg attttacgcc ggtctggaac
     7561 agtatgcctg ccggggccag cttctgtgtg gtggtgccgt attcacacgg tattgccgaa
     7621 gagatgcttt cgcacatcag ccaggaaaac gacaagctga atggggcgtt agacggtgcc
     7681 gcggggctgt gctggtatga agactcaaaa ttgcaaaccc cgctgtttgt cggtcagttt
     7741 gatggcactg ccgaacaggc gcaattgcca gggaaactgt ttacgcaaaa tattggtgcg
     7801 cacgaaagca aagcgccaga aggtgttttg ccgataagcc agactcagca gggcgaagcg
     7861 caaatctggc gtcgcgaagt gagttcccga tacggtcagt atccgaaagc gcaggcggcg
     7921 caacccgatc aattaatgtc ggattatttt ttccgcgtgt cgctggcgat gcaaaacaaa
     7981 acgctgcttt tctccctcga tgacacgctg gttaataacg ccctgcaaac actgaataaa
     8041 aaccgcccgg caatggtgga tgtaataccc actgatggca tcgttccgct ctatatcaat
     8101 ccacaaggcg tggcgaaact gctgcgtaac gaaacgttga ccagtctgcc gaagaatctc
     8161 gaaccggttt tttataacgc cg
//
LOCUS       DAZPEP010000171         8157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZPEP010000171 DAZPEP010000000
VERSION     DAZPEP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.172"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..254
                     /locus_tag="ACHUT9_003691"
     CDS             <1..254
                     /locus_tag="ACHUT9_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1350074.1"
                     /translation="RGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRY
                     FLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHUT9_003692"
     CDS             complement(303..1559)
                     /gene="nupG"
                     /locus_tag="ACHUT9_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFR1350075.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHUT9_003693"
     CDS             complement(1761..2840)
                     /gene="mltC"
                     /locus_tag="ACHUT9_003693"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFR1350076.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2905..3180)
                     /locus_tag="ACHUT9_003694"
     CDS             complement(2905..3180)
                     /locus_tag="ACHUT9_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFR1350077.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHUT9_003695"
     CDS             complement(3208..4260)
                     /gene="mutY"
                     /locus_tag="ACHUT9_003695"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFR1350078.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4421..5140
                     /gene="trmB"
                     /locus_tag="ACHUT9_003696"
     CDS             4421..5140
                     /gene="trmB"
                     /locus_tag="ACHUT9_003696"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFR1350079.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5140..5466
                     /locus_tag="ACHUT9_003697"
     CDS             5140..5466
                     /locus_tag="ACHUT9_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFR1350080.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5650..6369
                     /locus_tag="ACHUT9_003698"
     CDS             5650..6369
                     /locus_tag="ACHUT9_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFR1350081.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6545..7591
                     /gene="ansB"
                     /locus_tag="ACHUT9_003699"
     CDS             6545..7591
                     /gene="ansB"
                     /locus_tag="ACHUT9_003699"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFR1350082.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7708..>8157
                     /locus_tag="ACHUT9_003700"
     CDS             7708..>8157
                     /locus_tag="ACHUT9_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFR1350083.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNY"
BASE COUNT         2088 a         1987 c         2161 g         1921 t
ORIGIN      
        1 ttcgcggtga agtggagttg gtgcggattc gtgatgccga agggcgaatt gcggcagaag
       61 gggcgttgcc ttatccacct ggcgtgcttt gcgtggttcc cggggaagtc tggggtgggg
      121 cggttcaacg ttatttcctt gcgctggaag aaggcgtgaa tttgttgccg ggattttcgc
      181 cggagttgca aggtgtctat agcgaaaccg atgcggatgg catgaaacga ttgtacggtt
      241 atgtgttgaa gtaagaataa aaaaacgggt caccttctgg cgacccgttt ttctttgcgt
      301 aattagtggc taaccgtctg tgtgcctgtc gggacacgaa cgtgtttata tttgaacatc
      361 gccatgaaag cgaaggccag aaccacggaa taaccagcga aaatcaacca tacggtctgc
      421 cagtcggtaa tgccgttttg ggtgtacatc tcaacaactt taccgctcac gatgccgccg
      481 aggatacagc cgaagccgtt agtcatcatc aggaacatcc cttgtgcact ggcgcgaatt
      541 gccgggctaa cttctttttc gacaaacacc gaaccagaga tgttgaagaa gtcgaatgcg
      601 caaccgtaaa cgatcatcga cagtaccagc agtacagtac cgaacggagt cgggtcgccg
      661 taagcaaaca gcgcaaaacg caggatccac gccacaatac tgatcatcat tacgttctta
      721 ataccgtagc ggcttaagaa gaacgggatg gtcagaatga acagggtttc agagatctgc
      781 gaaatcgaca tgatgattga cgcatgctgc acgatgaagc tgctggcaaa catcggatct
      841 ttgtcgaagc tgtgcaggaa ggtattaccg aacatgttgg taatctgcag ttccgcgccc
      901 agcagcattg agaagatgaa gaagattgcc atacgcttgt ttttaaacag cgcgaatgca
      961 tcgaggccca gcagggttgt ccagctctga ttcgcttgct gtttagcaac cggaatatgc
     1021 ggcagagtca gggtaaacag aaccagaatg gcggaaagtg ctgcgccaat atacagctgc
     1081 atgtggctta attcgaagcc agacaggctc accacccaca ttgccatgat aaagccgatg
     1141 gtgccccaga tacggattgg cgggaagtca gtaacgatat ccatcccggc attttgcagg
     1201 cgatagtaag agatggtgtt gattaaccca agcgttggca tataagcaaa cgagttaatc
     1261 aatatcacaa ggaacatcgc ttccggcgtc gtgacctgtg ccgccatgaa cagcgtgata
     1321 gcgccaatgg tgtggcaaat ggcatatacc catttcgcac ttaaccattt gtcggccaca
     1381 atccccagca gcgcaggcat aaataccgcg gcgataccca gtgagctata aactgcgcca
     1441 atagaagcac cgtcaaactt cagggtaaca aacatatagg agccgagggt cgtcagccaa
     1501 cttccccaca gacagaactg cagaaaagag aggattttca gctgcagctt aagattcatg
     1561 ttaatttcct cacattgtga tgcggatgag tgtttgaaaa agcacatttg accgtgcgag
     1621 atgatgggat tctatcaact tcgcggactg gttttttgtt acctgtggca aataattgca
     1681 aacattccca atttgcaaaa cgaaattgtg atgtggataa catttttgcc cctgagcatc
     1741 gtcaggggcg gttaatggaa ttatcggcgg cggtaggatt tttgcgcggt attcacttta
     1801 taaagataac ggcgagattc cgcagaggga tggcgggtcg tcagcgtctg ataaacatcg
     1861 cccggcgtca tggtgttaat aatattggca gcctgaatct tatcattcga aaagactcgc
     1921 agcacgctgc ctgcgccgcc gttataggcg gtgatgacgg cataacgccg cgatgttggg
     1981 ttatcaattc cgccgagata aacattgttc agcatcgcca gatacgcggt gccggtatca
     2041 atattgctgg caggatcaaa caagaaactg cggctcggcg tgccggattt cccctgcgag
     2101 cggaacacat ctttcccggc agtatgttgt accacctgca ttaatcccag cgcatcggaa
     2161 cggctgaccg catacgggtt aaaggaagat tcggtctgca taattgccag aatcagcgac
     2221 tcatcaacgc catatttccg tgacgcctgg cggaccatgc cgagatattt gtgcgcacgt
     2281 ttatcaaggt ggttcggtac catgttaatg gtgacgctgt agatgatacg cagcccgtta
     2341 ctgcggcttt gcagacggtt tttcagcaga taatccgcga agttgcttgc gcgaccttcc
     2401 cagcgaatcg gctgcccggt gttgtccacc acctgaccgt aaaggaaagg ttctttcgaa
     2461 atcgtaatat catcaacgtc ggaatagaga tcgaccgaac tcggatcgtc acccatcagc
     2521 aacgttttga taattgcccg gcgcaaatgc gcggcaggtt ctgtcccggc gatagtttcg
     2581 atagtaatcg taccgtcatc gaagttgatg tggctgcggg tctgatattg atcggtgtat
     2641 ttcacgtagt ccttaggacc ggcgatcacc acctctttga atccccagat gttctcaata
     2701 ttgtgggcaa attgccccat cagaatatca aaaccgttgg tatctttgac ccaggcttcg
     2761 ttataggtat cgcctttttt ggtcgtcgaa caggagatga gcaacggcgc aatcaaagcc
     2821 agcgcgagat attttttcat cattccgggt gcgtgttgtg tttgttttta tgcaagttgc
     2881 cggaggcgtg ctccggcact gtttttattt tttatcttcc ggcgtatagc cctcgatatg
     2941 cacctcttta ccctcgaaca ggaagttgac catctcctgc tcaagcagct tgcggtgctc
     3001 ggcattcatc atgttgagtt tcttttcatt aatcagcatg gtttgcttgt gctgccactg
     3061 cgcccaggct tctttggaga tctcgttata gatgcgtttt cccagctcgc cggggtacag
     3121 ctgaaaatcc tgaccttctg cttcacgttg caggaaagta caaaaaatcg ttctgctcat
     3181 aaatcatcct ctttatcgac tcacgcgcta aaccggcgcg ccagtgcgta actgctgtaa
     3241 caaacgctcc acgggagccg ccaggccaac tgacggcggt tgcgctaagt tataccagag
     3301 cgcattgcct tcatccatgc agccggtgaa tgacgacacg ggaagccaca taggcacaat
     3361 atctaagtgg aaatggctga aggtatgccg aaacgcggtc agttgcgtca ggttatcggc
     3421 agcaatctgc cgttgcgcca gccactgccg caaactttct tcgtcggcaa actgcgggaa
     3481 acagtataaa ccgccccaca atccgctcgg cggacgctgc gccagcaata cttcatcttc
     3541 gtgctgtaac agcaaaaagt agccggtgcg ctccggcagc gtctgtttcg gttttttgcc
     3601 cggataaagc gaccagctat tattggcggc ggcaatacat ccgttttgta gcggacagag
     3661 cgaacatttc ggcttcgagc gcgtacaaat catcgcgccc aaatccatca tcgcctgatt
     3721 aaaccgttcc acgccgaccg cgggcgtcac ctgctcgctc aaactccata atttattctc
     3781 gacctctttt ttcccaggcc agccgcttac agcatagcag cgcgccagca cgcgtttgac
     3841 gttaccgtcg agaatcggaa agtgcttacc cagagaaagc gagagaatcg cgcctgcggt
     3901 ggaacgcccg acgcccggta acgccgcgac ttcttcaaag gtttccggga atttaccgcc
     3961 gtgtaaggtc gccacttgtt gtgccgcttt atgcagattg cgcgcgcggg cgtaatagcc
     4021 aagcccggtc cacaagtgga gaacttcgtc gagcggcgca ttggcgagat cggtcaccgt
     4081 cgggaagcgc gccataaagc gttcaaaata ggggataacg gtcgcaacct gagtttgttg
     4141 caacatcact tctgagagcc atactttgta gggcgtcttg tcaatttgcc agggcagcgt
     4201 ttttcgcccg tatttatcgt accagtccag aacctgggct gaaaattgcg acgcttgcat
     4261 ggtcaccgaa ttcactgttg ttgggggcaa gattgcagca cagcgacggc agggtgtaaa
     4321 ccggaacttt ccgcagccac ggccttatca tgcttgcatc cggcaactaa ctttggataa
     4381 tgcccgtttt cagaacactt tcacaagcga ctaaatcttt atgaaaaacg acgtcatttc
     4441 accggaattt gatgaaaacg gccgcccact gcgccgtatc cgtagttttg tgcgccgcca
     4501 ggggcgactg accaaaggcc aggaacatgc gctggaaaac tactggccgg tgatgggcgt
     4561 tgagttcagc gaagatatgc tggatttccc cgcgcttttt ggccgtgaag cgccggtgac
     4621 gcttgagatt ggttttggca tgggggcgtc gctggtggca atggctaaag atcgccctga
     4681 gcaggacttc ctcggcattg aagtgcattc accgggcgtt ggtgcgtgcc tggcttctgc
     4741 gcatgaagaa ggtttaagca acctgcgcgt gatgtgtcac gatgcggttg aagtgctgca
     4801 taaaatgatt cctgacaatt cattgcgcat ggtgcagctc tttttccctg acccgtggca
     4861 caaagcgcgc cataataaac gccgtatcgt acaggtgccg tttgccgaac tggtaaaaag
     4921 caaactgcag ctggggggtg tattccatat ggcgaccgac tgggaacctt atgcggaaca
     4981 tatgcttgaa gtgatgtctt ctattgacgg ttataaaaac ctgtcagaga gcaatgatta
     5041 cgtgccgcgt ccggcatcac gtccggtgac gaaatttgaa caacgtggtc atcgtcttgg
     5101 tcacggagta tgggacttaa tgttcgagag ggtgaaataa tggcaaagaa ccgtagccgt
     5161 cgtctgcgta aaaaaatgca catcgacgaa ttccaggaat taggattttc ggtggcatgg
     5221 cgattcccgg aaggtacatc ggaagaacag attgataaaa ccgttgatga ttttattaac
     5281 gaggttatcg aaccgaacaa actggccttt gacggcagcg gttatctggc ctgggaaggt
     5341 ctgatctgca tgcaggaaat cggcaaatgc accgaagaac atcaggcgat tgtgcgtaag
     5401 tggctggaag agcgcaaact ggatgaggta cgcaccagcg aacttttcga cgtttggtgg
     5461 gactaagaaa gcatacgggc gatgacaaat gcaaaactgc ctgatgcgct acgcttatca
     5521 ggcctggaaa gatgcacgat cgagtaggcg ggataaggtg tttacgccgc atccggcatg
     5581 gaaaacgcgt actttgttat caatctgggg ccagcaaatg ctggcctgat tttttcttga
     5641 gggaagacta tgatgcgcaa aatgctgctg gcggcagcac tttcagtgac ggcaatgacc
     5701 gctcacgccg actaccagtg cagcgtcacg ccgcgtgacg atgtgattgt cagcccgcaa
     5761 accgtgcagg tgaagggcga aaacggcaat ctggtgatca cgccagacgg caacgtgatg
     5821 tataacggta agcaatattc cctgaatgcc gcccagcgcg agcaggcgaa ggattatcag
     5881 gctgaactac gcagcacgct gccgtggatt gatgaaggcg cgaaaagccg cgtcgaaaaa
     5941 gcccgtattg cgctggataa aattatcgtt caggagatgg gcgaaagcag caaaatgcgc
     6001 agccgtctga ccaaacttga tgcgcagctg aaagagcaga tgaaccgcat tatcgaaacg
     6061 cgcagcgatg gcctgacgtt tcactataaa gccattgatc aggttcgcgc cgaaggccag
     6121 caattagtga atcaggcaat gggtggaatt ttacaggaca gcattaatga aatgggcgcg
     6181 aaagcggtgc tgaaaagcgg cggtaaccca ttacagaatg tgctgggaag cctggggggg
     6241 ctgcaatcct caatccaaac agagtggaaa aagcaggaaa aagatttcca gcagtttggc
     6301 aaagatgttt gtagccgcgt tgtgactctg gaagatagcc gcaaagccct ggtcgggaat
     6361 ttaaaataat cctctatttt aagacggcat aatacttttt tatgccgttt aattcttcgt
     6421 tttgttacct gcctctaact ttgtagatct ccaaaatata ttcacgttgt aaattgttta
     6481 acgtcaaatt tcccatacag agctaaggga taatgcgtag cgttcacgta actggaggaa
     6541 tgaaatggag tttttcaaaa agacggcact tgccgcactg gttatgggtt ttagtggtgc
     6601 agcattggca ttacccaata tcaccatttt agcaaccggc gggaccattg ccggtggtgg
     6661 tgactccgca accaaatcta actacacagc gggtaaagtt ggcgtagaaa atctggttaa
     6721 tgcggtgccg caactaaaag acattgcgaa cgttaaaggc gagcaggtag tgaatatcgg
     6781 ctcccaggac atgaacgata atgtctggct gacactggcg aaaaaaatta acaccgactg
     6841 cgataaaacc gacggcttcg tcattaccca cggtaccgac acgatggaag aaaccgctta
     6901 cttcctcgac ctgacggtga aatgcgacaa accggtggtg atggtcggcg caatgcgccc
     6961 gtccacgtcc atgagcgcag acggtccatt caacctgtat aacgcggtag tgaccgcagc
     7021 tgataaagcc tccgctaatc gtggcgtgct ggtggtgatg aacgacaccg tactggacgg
     7081 tcgcgatgtc accaaaacca acaccaccga cgtagcgacc ttcaagtctg ttaactacgg
     7141 tcctctggga tacattcaca acggtaagat tgactaccaa cgtaccccgg cacgtaagca
     7201 caccagcgat acgccattcg atgtctctaa gctgaatgag ctgccgaaag tcggcatcgt
     7261 ttataactac gctaacgcat ccgatcttcc ggctaaagca ctggtagatg cgggctatga
     7321 tggcatcgtt agcgctggtg tgggtaatgg taacctgtat aaatccgtgt tcgacaccct
     7381 ggcaaccgcc gcgaaaaacg gcactgcagt agtgcgttct tcccgcgtac cgacgggtgc
     7441 taccactcag gatgctgaag tggatgatgc gaaatacggc ttcgtcgcct ctggcacgct
     7501 gaacccgcaa aaagcgcgcg tcctgctgca gctggctctg acgcaaacca aagatccgca
     7561 gcagatccag cagatcttca atcagtacta atcacctcgc cccggtatcg tgccggggct
     7621 ttttcacttc agacttacgt ccattgccaa ttttcattac cctaatgata atcaccggaa
     7681 taaattattc cgcgtgaggt ttttcgggtg aaaaagcaat ggattgttgg cacggcactg
     7741 cttatgttga tgactggtaa tgcctgggca gatggcgaac cgccaactga aaatatctta
     7801 aaagatcaat tcaaaaagca gtatcacggc attctcaagc ttgatgctat caccttaaaa
     7861 aatcttgatg ctaagggtaa tcaggccacc tggtcagcgg aaggcgatgt ctcttccagt
     7921 gacgatctct atacctgggt cggtcagttg gcagattacg aactgctcga acagacctgg
     7981 acgaaagata aaccggtaaa attctcggcg atgttaacca gtaaaggaac gcccgcgtct
     8041 ggctggtcgg tgaactttta ctcttttcag gcggcagcca gcgatcgtgg gcgggtggtc
     8101 gacgatatca aaacgaataa taaatatctg atcgtgaata gcgaagattt caattat
//
LOCUS       DAZPEP010000172         8138 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZPEP010000172 DAZPEP010000000
VERSION     DAZPEP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.173"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..188
                     /locus_tag="ACHUT9_003701"
     CDS             <1..188
                     /locus_tag="ACHUT9_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350084.1"
                     /translation="MLNPTKDTNWNSTCIYKSRHKMLPINLTQETLFSSKSHGKYALF
                     PIFTASWRATRIKNIGI"
     gene            411..908
                     /gene="ubiC"
                     /locus_tag="ACHUT9_003702"
     CDS             411..908
                     /gene="ubiC"
                     /locus_tag="ACHUT9_003702"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFR1350085.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUT9_003703"
     CDS             921..1793
                     /gene="ubiA"
                     /locus_tag="ACHUT9_003703"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFR1350086.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUT9_003704"
     CDS             complement(1948..4371)
                     /gene="plsB"
                     /locus_tag="ACHUT9_003704"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFR1350087.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUT9_003705"
     CDS             4542..4910
                     /gene="dgkA"
                     /locus_tag="ACHUT9_003705"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFR1350088.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUT9_003706"
     CDS             5020..5628
                     /gene="lexA"
                     /locus_tag="ACHUT9_003706"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFR1350089.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUT9_003707"
     CDS             5701..7026
                     /gene="dinF"
                     /locus_tag="ACHUT9_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFR1350090.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7142..7351
                     /locus_tag="ACHUT9_003708"
     CDS             7142..7351
                     /locus_tag="ACHUT9_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFR1350091.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUT9_003709"
     CDS             complement(7393..7908)
                     /gene="zur"
                     /locus_tag="ACHUT9_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFR1350092.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1837 a         1988 c         2221 g         2092 t
ORIGIN      
        1 atatgttgaa tcctacaaaa gataccaatt ggaattccac gtgtatctat aaatccagac
       61 ataagatgtt gcctattaat cttactcagg aaacactttt tagctccaaa tctcatggta
      121 aatatgcgct tttcccaatt tttactgcga gttggcgagc tactcgtata aagaatatag
      181 gtatttaagt aaaggaaaac atcaccgttc ctggcatcat gggcggtgat gcccctacgg
      241 ttgccctcgc cagcacgggc atcggcaaag cgtaaggttc aacagcgttt taccacttca
      301 tgcgattgtt gcgtttttgt tgcgcattag atcacttaat ttgctttaca tctcccgtaa
      361 acacttttct gcgatacaat gcctttacgt tatgtaacgg agagttcggc atgtcacacc
      421 ccgcgttaac gcaactgcgt gcgctgcgct attttaaaga gatccctgcc ctggattcgc
      481 aactgctcga ctggctgttg ctggaagatt ccatgacaaa acgttttgaa cagcagggaa
      541 aaacggtgag cgtgacgatg atccgcgaag ggtttgtcga gcagaatgaa atccccgaag
      601 aactgccgct gctgccgaaa gagtctcgtt actggttacg tgaaattttg ttatgtgccg
      661 atggtgaacc gtggcttgcc ggtcgtaccg tcgttcctgt gtcaacgtta agcgggccgg
      721 agctggcgtt acaaaaattg ggtaaaacgc cgctaggacg ctatctgttc acatcatcga
      781 cattaacccg ggactttatt gagataggcc gtgatgccgg actgtggggg cgacgttccc
      841 gcctgcgatt aagcggtaaa ccgctgttgc taacagaact gtttttaccg gcgtcaccgt
      901 tgtactaaga ggaaaaaaat atggagtgga gtctgacgca gaataagctg ctggcgtttc
      961 atcgcttaat gcgtacggat aagccaattg gcgcgttact gctgctctgg ccaacattat
     1021 gggcgttgtg ggtggcgaca ccgggcgttc cccagctctg gatcctggcg gtgtttgtcg
     1081 cgggtgtctg gctgatgcgc gctgccggat gtgtggtgaa tgattatgct gaccgcaagt
     1141 ttgatggtca tgttaagcgc acggcgaacc gaccgcttcc cagcggcgcg gtaacggaga
     1201 aagaggcgcg cgcgctgttt gtagtgctgg tattgatttc atttttactg gtgctgacgc
     1261 tgaatacgat gaccattctg ttgtcgattg ccgctctggc gttggcgtgg gtgtacccgt
     1321 ttatgaagcg gtatacccat ctcccgcaag tggtgctggg cgcggcgttt ggctggtcga
     1381 ttccaatggc ttttgccgct gtgagtgagt cggtgccatt gagttgctgg ttaatgttcc
     1441 tcgccaatat tctctgggcg gtggcttacg acacgcagta tgcaatggtt gaccgtgacg
     1501 atgatgtgaa gattggtatt aaatccaccg caatcctgtt cggccaatac gataaattga
     1561 ttattggtat tttgcagatt ggcgtactgg cactgatggc gatcatcggt gagttaaatg
     1621 gcttaggctg gggatattac tggtcaattc tggtggctgg cgcgctgttt gtttatcaac
     1681 aaaaactgat tgccaaccgc gagcgtgaag cctgctttaa agcatttatg aataataact
     1741 atgttggtct ggtactgttt ttaggactgg caatgagtta ctggcatttc tgatgatgta
     1801 aaaaagccgg atgatcatcc ggctttcttc tgggttgcct gatgcgctgc gcttctcagg
     1861 cctacacaac acatcgcaat ttattgaatt tgcagattat ggaaggccgg ataaggcgtt
     1921 ttcgccgcat ccggcaattc tctctgatta cccttcgccc tgcgtcgcac tctcaatcgt
     1981 caaacgcacg tctgatgtaa tcaactccgc cagcaactga taaaccttca tcgtttctgc
     2041 cggttcggcg tcgccgctat cgctgatata cccttcatca cgcagcgtca gcaccagaga
     2101 actgaacacc gccttgtcga agaactccgg cgcgttgatg ccgtgcagca cggagagacg
     2161 ttgcgcaacg gtgcggctct ctttctccag cgtaccgcgg ttgatcgacg ggttggcact
     2221 caacaaccag aaggtgatgg cataacgttg cagcgtttcg cgcgcgcctg cggccagcag
     2281 ctgtaggggg cgagaatgcg ccgggttgat atgcaactca tcatcttgca gggtaatcag
     2341 cccctgacgt tgcatctcat ttgccagcgc atcaataacg tccggcaact cgtcgcgatc
     2401 ccagcgcagg aacagctccg ctttcagcat tgggtaaagc acattgacgt gctccatcaa
     2461 tacgtcgcgg gagatgtggc gatgctgggt gacaattgcc gccatcagcg aaggcagcac
     2521 caacatatgc gcaatgttgt tgcgatagta ggtcatcagc accgcttgct cgcgcggcag
     2581 aatgatgatg tcgccgatag tgtctttctc gacttcaaac ttgttcattt gcagcgcgtg
     2641 atcgataagc tcgctggcgc tggctgaagg aacggtagag tccgtggagt aaggtacgtt
     2701 gcgcatcaga tccaggtagc agttgagttg ctcggttaac tgctcgcggg tgagtgagcg
     2761 ctgacgtgat gccagaagcg cagtacaaca caggttcatg gcgtttgccg cgcctgcgtt
     2821 gttaatgcgt accatcagat cggcagcaat attattgacc gtcggcgtta accaggccgg
     2881 acgcaccgct tcgatgggat cgatagattc acgccagtct ggtacgtgct ggttaaggta
     2941 ggtcatcaac ggcattggtt caccgaagtt gacgtaaccc tgaccgagat tacgcagctt
     3001 gcttaaaccg cgcagcatct gcggcaggct ctctttctct ttcgtcgcgc cgcgcagttc
     3061 tttggcgtaa gtacccactt ccatgacgtg ctcataaccg atatagatcg gaatcagcgt
     3121 aatcggacgc gtgccgccac gcagcatcgc ctgaatggtc atcgacagcg taccggtttt
     3181 cggatccagc aaacgccccg tgcgggaacg accgccttcc acgaagtact cgacggaata
     3241 accacggctg aacagttcgc cgagatactc acggaaaacg gtggaataaa gtttattgcc
     3301 tttaaaagta cggcgaataa agaacgcccc cagacggcgg aaaatcggcc cggccggcca
     3361 gaagttcagg ttgatcccgg cggcgatatg cggcggcacc agcccctggt ggtacagcac
     3421 gtaagaaagt agcaggtagt ccatgtgact gcggtggcaa ggcacataca ccagctcatg
     3481 accgtcgtgg gccagctggc gaacgcgctc ggcgttatgg acgttgatgc cctgataaag
     3541 tcggttccag gtgaagccca gaatgcggtc agtcaggcgg atcatctcgt aagagaaatt
     3601 cgccgcaatc tcttccatca gcgcaatcgc gttctgctgc gctttttcat gggagatttt
     3661 tttgctgcgc gcttcatctt ctaccgcttt ggcaatggcg cgggaggcga gcagcttatt
     3721 aaacagatcc tgacgagcag gcagacgtgg gcctacggca gccagacgtt gacgggcaaa
     3781 gtgcatacgc gccacgcgcg ccagtttctg tgcgatagtt ttatccgtgc cgtgttcatc
     3841 cgccatacgg cgcagcgaaa ctgacggcga gaaacgcaca aaactgtcgc gaccgagcca
     3901 cagtacggcg aaaaatttct gtacgccgtt aagcatacgc agcggcgggt ttacttcgcc
     3961 tttttcacgc cccggcgcgc gaccaaacat caccgatact ggcaccatct gcacatccag
     4021 atttgggttg ctacggtgca aatcgagata gtcgtggaac agcttaatag actcttcttt
     4081 cggcgtgtaa taggtgaata cacgcggccc gccgtgaatg aacacatagc gcggcagtag
     4141 cgtgccgtcg atttctagcg gctctaacgg gtcaggcaag tcatgagcca gacactgggc
     4201 gcgtaacgtc agcaaatctg ctttcgagtt gtacggtaaa acgtacataa ttggacgaga
     4261 ggtatccagc cccagttccg gggcaggatc tgccggaata gacttgcttt ttaccaggat
     4321 gcttaatggt aaattcagta atttgtagta aattcgtggc cagccggaca taaacgatgt
     4381 aaagcctctg gttaataatg caaatgcgcg gcaaggatag cagaaagtca tgggaaattc
     4441 tgtggtatcc gctcatgttt cgcgcggcgt tacgcaaacc cgaatcatcg gatttaacgg
     4501 tacactgata ttgacgctca taatgtaaaa aggttctttc aatggccaat aataccactg
     4561 gattcacccg aattatcaaa gctgctggct attcctggaa aggtttacgc gctgcatgga
     4621 tcaacgaagc ggcattccgt caggaaggcg tagcggtatt gttggcggtg gtcatcgcct
     4681 gctggctgga tgtggacgca attacccgcg tgctgcttat cagctccgtg atgctggtga
     4741 tgattgtgga aattctcaat agcgccatcg aagcagtggt tgaccgaatt ggctctgaat
     4801 accatgagct ttccggacgc gctaaagata tgggatccgc cgcggtgctg attgcgatta
     4861 tcgtcgccgt gataacctgg tgcatcctgt tatggtcgca ttttggataa cccttccaga
     4921 tttcgataaa tctctggttt attgtgcagt ttatggttcc aaaatcgcct tttgctgtat
     4981 atactcacag cataactgta tatacaccca gggggcggaa tgaaagcgtt aacggccagg
     5041 caacaagagg tgtttgatct catccgtgat cacatcagcc agacaggtat gccgccgacg
     5101 cgtgcggaaa tcgcgcagcg tttggggttc cgttccccaa acgcggctga agaacatctg
     5161 aaggcgctgg cacgcaaagg cgttattgaa attgtttccg gcgcatcacg cgggattcgt
     5221 ctgttgcagg aagaggaaga agggttgccg ctggtaggtc gtgtggctgc cggtgaaccg
     5281 cttctggcgc agcagcatat tgaaggtcat tatcaggtcg atccttcctt gttcaagccg
     5341 aatgctgatt tcctgctgcg cgtcagcggg atgtcgatga aagatatcgg cattatggat
     5401 ggtgacttgc tggcagtgca taaaactcag gatgtacgta acggtcaggt cgttgtcgca
     5461 cgtattgatg acgaagttac cgttaagcgc ctgaaaaaac agggtaataa agtcgaactg
     5521 ttgccagaaa atagcgagtt taaaccaatt gtcgttgacc ttcgtcagca gagcttcacc
     5581 attgaagggc tggcggttgg ggttattcgc aacggcgact ggctgtaaca tatctctgag
     5641 accgcgatgc cgccaggcgt cgcggtttgt ttttcatctc tcttcatcag gcttgtctgc
     5701 atggcattcc tcacttcatc tgataaagca ctctggcatc tcgccttacc catgattttc
     5761 tccaatatca ccgttccgtt gctgggactg gtcgatacgg cggtaattgg tcatcttgat
     5821 agcccggttt atttgggcgg cgtggcggtt ggtgcaacgg cgaccagctt tctctttatg
     5881 ctgttgctgt ttttacgcat gagcaccacc gggctgactg cgcaggctta tggtgccaaa
     5941 aatcctcagg cattagcccg tgcgctggtg caaccgttgc tgttggcgtt gggggctggg
     6001 gcgttaattg cgctgctgcg tacaccgatt atcgatctgg ctctgcatat tgttggcggt
     6061 agtgaagcag tcctggaaca ggcgcggcgc tttcttgaaa tccgctggtt aagcgcaccg
     6121 gcgtcgctgg cgaatctggt attactcggt tggttactcg gcgtgcaata tgcccgtgcg
     6181 ccagtaattt tgttagtggt cggcaatatc ctcaacattg tgctggatgt ctggctagtg
     6241 atggggctgc atatgaacgt gcagggcgcg gctctggcga cggttattgc ggaatatgca
     6301 acattgctga ttggtctgct aatggtgcgt aaaatcctca aactacgcgg aatttccggc
     6361 gaaatgtcga aaactgcctg gcgaggaaac ttccgtcgct tgctggcgct taaccgcgat
     6421 atcatgctgc gttcgctgtt gttgcaactc tgtttcggcg cgatcaccgt acttggcgcg
     6481 cgactgggga gtgacattat cgctgtaaac gcggttctga tgacgctact cacctttacc
     6541 gcctatgcgc tggatggttt tgcctacgcg gttgaagcac attccggtca ggcatacggt
     6601 gcgcgcgacg gtagccagtt gctggatgtc tggcgggcag cgtgccgcca gtcggggatc
     6661 gtagcgttac tattttcggt ggtttatttg ctggcagggg aacacatcat tgcgttgctg
     6721 acgtcgttaa cccagattca gcagctggct gaccgctatc ttatctggca ggtgattttg
     6781 ccgttggttg gcgtctggtg ttatctgctg gacggcatgt ttataggcgc aacgcgtgcc
     6841 gccgaaatgc gtaacagtat ggcggtggcc gccgcaggtt ttgcgctgac gctccttacg
     6901 ctgccgtggc tgggtaatca tggtttgtgg ctggcattaa ccgtctttct ggcgttgcgc
     6961 gggctttctc tggcggctat ctggcggcgt cactggcgta acggtacctg gtttgccgca
     7021 acgtgacggt taaaaattct gaataaatta tcctaagcca aattgctgac tacacttaat
     7081 ctcacgttca gaagaaaagt gaacgtactc tcattcacaa cctaacgatg aggtcttgat
     7141 tatgaataaa gatgaagccg gcggtaactg gaaacagttt aaaggtaaag tgaaagagca
     7201 atggggcaaa ctgaccgatg atgatatgac gatcattgaa ggtaaacgtg atcaactggt
     7261 cggtaaaatc caggaacgtt atggttatca gaaagatcag gcagaaaaag aggtcgtgga
     7321 ttgggaaacc cgcaatgaat atcgctggta attaatccct cctgcccgac gtgtacaagg
     7381 atgtacgccc tcttaacgcg gtttcttttt cacctgcaca gagtgatcat gctggcactg
     7441 ttcaggatga cgacacgctt ccacttccac gcatgccgca cagagcccat gtgcttcaat
     7501 cacattatgc cgcagggcaa accccatttt tgccgccagc gtatgcataa tgtcttccac
     7561 gccttctgca cactcttctt tcaccgcgcc gcagcgatcg catataaaca tggctgacgt
     7621 atgcgtgggc tggtcgaaca gatgacagag cacataactg ttggtggatt ccaccttatg
     7681 cacaaaacct tgctcaagca gaaaatccag cgcgcgataa accgttggcg gcttggcttg
     7741 cggttcagct tcgcgcagta aatcaagcag atcataagcg ctgatagcgc catcctgcag
     7801 actcatcagg cgcaacactt ccaggcgctg tggggtcagg cgcacattac gctgcgcgca
     7861 gattttttca gcctgcgcta ataactcctg cgttgtggtc ttttccatcg ggcacctcaa
     7921 agtgggggtt aagaatgtcc ttactttacc atgttccagg aaaaacaccg agaaccctta
     7981 ttgttgccgt aatgttgatt ttctgttttg taggtaaggt gttatgttgc cttgtcgtac
     8041 cattatcaac acgataataa ttaataattc atttttaaat aaattgcttc aagttaactc
     8101 gctatttatt taactgggtt taaacgcata cctgtagt
//
LOCUS       DAZPEP010000173         8061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZPEP010000173 DAZPEP010000000
VERSION     DAZPEP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.174"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1338
                     /gene="yjeM"
                     /locus_tag="ACHUT9_003710"
     CDS             <1..1338
                     /gene="yjeM"
                     /locus_tag="ACHUT9_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFR1350093.1"
                     /translation="MMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWM
                     VSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIA
                     RITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVF
                     AIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLS
                     NGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTL
                     CYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAF
                     FNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVTF
                     ANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(1489..4812)
                     /gene="mscM"
                     /locus_tag="ACHUT9_003711"
     CDS             complement(1489..4812)
                     /gene="mscM"
                     /locus_tag="ACHUT9_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFR1350094.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4834..5802)
                     /gene="asd"
                     /locus_tag="ACHUT9_003712"
     CDS             complement(4834..5802)
                     /gene="asd"
                     /locus_tag="ACHUT9_003712"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFR1350095.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5899..6951)
                     /gene="rsgA"
                     /locus_tag="ACHUT9_003713"
     CDS             complement(5899..6951)
                     /gene="rsgA"
                     /locus_tag="ACHUT9_003713"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFR1350096.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7046..7591
                     /gene="orn"
                     /locus_tag="ACHUT9_003714"
     CDS             7046..7591
                     /gene="orn"
                     /locus_tag="ACHUT9_003714"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFR1350097.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7802..7877
                     /locus_tag="ACHUT9_003715"
     tRNA            7802..7877
                     /locus_tag="ACHUT9_003715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7835..7837,aa:Gly,seq:gcc)
BASE COUNT         1857 a         2086 c         2173 g         1945 t
ORIGIN      
        1 atgatggctg aaatgggagc tgcttatcgc aaagaagaag gtggtatcta ttcctggatg
       61 aataatagtg tcggaccacg ttttgccttc attggtacgt ttatgtggtt ttcctcttat
      121 atcatctgga tggtgagtac ctccgcgaaa gtttgggtac cgttctcaac attcctctat
      181 ggtagcgaca tgacccagca ctggcgtatt gccggactgg agcctacgca ggtggttggt
      241 ctgctggcag tagcatggat gattctggtc accgtcgttg cttcaaaggg gattaataaa
      301 attgcccgca ttactgcggt gggcggtatt gcagtaatgt gtctgaattt agtattgctg
      361 ttagtaagca ttactatttt gttattaaat ggtgggcatt tcgcgcagga tattaatttc
      421 cttgcatcac cgaacccggg ttatcagtcc ggtctggcaa tgctatcgtt tgtggtattt
      481 gctatttttg cctatggcgg aattgaagcg gttggtggtc tggtcgataa aacggaaaat
      541 ccagaaaaga actttgccaa aggtattgtt tttgccgcta ttgttatttc aatcggttat
      601 tcgctggcaa tatttttatg gggcgtcagc acaaactggc agcaggtatt aagtaatggt
      661 tccgttaacc ttggcaatat tacctatgtg ctgatgaaga gcctcggggt gacgctgggt
      721 aacgcactgc atttgtcacc tgaagcgtca ttgtcgctgg gcgtatggtt tgcgcgtatt
      781 accggacttt cgatgttcct cgcttatacc ggtgcgttct ttacgctttg ctattcaccg
      841 ctgaaagcca tcatccaggg gacgccgaaa gcgttgtggc cggaaccgat gacgcgcctg
      901 aatgcgatgg ggatgccgtc tatcgccatg tggatgcagt gcgggttggt tactgtcttc
      961 atcctgctgg tttcgtttgg tggcggtacc gcatccgcgt tctttaacaa gctgacgctg
     1021 atggcgaacg tgtctatgac gcttccttac ctgttcctcg cgctggcttt cccattcttt
     1081 aaagcacgtc aggatctcga cagaccattt gtgattttca aaacgcgtat gtcggcaatg
     1141 attgcgatgg tggttgtcgt actggtggtg acatttgcga acgtcttcac cattattcag
     1201 cctgtggttg aagccggaga ctgggacagc acattgtgga tgattggcgg ccctgtcttc
     1261 ttctcgctgt tagcgatggc gatttaccag aactattgca gtcgcatggc gaataaacct
     1321 gagttagctc tcgactgatc atatgaaatc ctgcaactca tcgtctctcc ggcgatgagt
     1381 tgtcaggaag taatgcgtat ggatgatact ctccagggcc ggtgataact gaagatgatg
     1441 ttaagctgct aaatgagcca cgttgacatc atttggcagc ttcatgcatt acaaacttcc
     1501 cgcctgacga cctttgcccg cagacgtcag cgttctcccc gtttgtttac cgttgaggct
     1561 ttccagacgc atctggaagg gcgggaatgg catatcgata ccgtgggcat ggaagccagc
     1621 cagaatcagc tggtggatct catggcgtag cggcatacgg tgacccatct cagcggcata
     1681 aatacgcagc tcgaaaatct gaatcccttg ttgcagatcc accaggaaga cttccggtgc
     1741 cgggttgtcg atcaccagcg agcagcgacg cgctgcggtg agcaggattt ccgtcacttc
     1801 ttcactgttg gcatcagcag gggccggaat cgtcaacact acgcgcgtga ccgagtcaga
     1861 gagcgaccag ttgataaact gctcggtaat aaacgccttg ttcggcacga ttatctcttt
     1921 acggtcccag tcgctgatgg tggtggcgcg ggtgttaatt ttcgtcacgc taccggtgag
     1981 atcgcgaatt gtcaccgtat cgccaatgcg aatcggtttt tcgaacagga taatcagacc
     2041 agagataaag ttggcgaaaa tttcctgcaa accaaaaccg agaccaacac cgagcgcggc
     2101 gaccagccac tgcaatttcg accactcaat accaatcatc gagaagccga ccagcccgcc
     2161 aatcagcatc agcagatatt tggtgatggt ggtgatggcg tagcccgtac ccggcgttaa
     2221 atccaggtgc tgcaaaatcg ccagttccag cagcgcgggc aggttgcgca ccagctgcgt
     2281 ggtgatgata aacaccagaa tggcaatcag caccgcaccg agggtaattg gctccagact
     2341 ttctacgccc tgtaccgtgg aggtgacatc ccacagcgaa atattttcga ggaagccgaa
     2401 agcggaatgg atttctgacc acagcacaat gacagaaagc agggcgatca acatcaaaat
     2461 tgagcgcacc agccgcaagg attgcgcact gatggcatcg agatcgactt cgctttcatc
     2521 gacttcaatt gctccttccg ggctactgtg atgatgcgct tcctcttcgc cacgcgcacg
     2581 ttgcgctaac atctctgccc ggcgatgctt cgcccggtca aacgccagcc tgcggcgctg
     2641 gatcagcatc cagcggcgga taacgtgata aaccaccagt agcaggaacc agatggcaac
     2701 cgaggtttca agcctcgcta acagcgcctg tgccgttgcc agataaccca cagccgacgc
     2761 cagaatggca accaacggcg cgccaatcat catgttccac agcatatggt tggtaatgtt
     2821 gtcgccgctg ccttctttgt tgagatacag cggaatcccg gcctttttca ggctgagggt
     2881 gaccaccgcc agcgcaccgc aaatgaggat aaagcaaagc cttcccagcg aaccggagaa
     2941 ttcacggtcg tcgaggttat cgaacatcat cagcgccata atcagcggca caataagccc
     3001 gatgctcatc aggtagtagc gcatcccacg agatacgcgt tcgcgcggcc agccaaaatg
     3061 agcgataaac aagccgttcg ggcgggcaaa ggtggcgcaa atcatcacta cccacagcag
     3121 cggcacggtg gccgttacac catcgccaat cgcgaccgcc agcggataag gccacgcctc
     3181 gcgcaagccg taacccagcg tcatccacag caccggtaac ggtgacgcga cgagaatcga
     3241 ccagaaaaga gtgcgcaacg tcagccagaa gtgatcctga gtcactttgc caactttcgc
     3301 cgccgaacgt tcaagaaaac gggtgaaata gcggcgcgag taaatactgc aaccgaccag
     3361 aatcaacgcg ccaaacagcg gcaaaattgt ctctttgctg gtcagcatca tcacactggc
     3421 tttgcccaac tgactgaagg tgtccagcga aatgagacga cgcagatcct gggcgatttc
     3481 cagcggccag gcgatggtca tcgggcgcac gtcagaggtc cagaacagat agcggtgcgt
     3541 tgcttcgttc acctctttca gcgcatcctc cagttgcccg ttggagactt tcagcttggt
     3601 cagttccaat agtagcgtgt cgccaccctg caacaatgag ttcagcaact cacgctgagt
     3661 gcgcagttgt gcttccagaa tacggttttg ctcggcggtc agcggctgac cgtcggcctg
     3721 atggatttgc cgtagcagcg gctgtttatt aagcagatcc tcataacgta accgttgcac
     3781 acgcaactgc gccatttcgg tatcaagctg ttgtggtttt ggcatttctg gtagccgtgc
     3841 cacctgcgct cgcaacgctt caccgagcag attggacgat cccagccatt gcgactgttc
     3901 acgcagcgta ttcaacgcct gccggacctg taacgtctgg ctggcagcct gacgctgttg
     3961 cgaggcaacg agatccatcc gctgcgcctg ttgattcagc gctgccgata gttcgcggtt
     4021 aattttgaat tgcgcgacga tatctttcgg caaatcggca ctgttttctg ccagtaattc
     4081 ggtactttcc agcgcccgct ccgcctcaag ctgacgttgg ctattcaact gattacgtaa
     4141 ggcctgcaaa tacgcatcca gttgctggct ctctttttcc gcaagctctg agcgtaagcg
     4201 cgctaattct tggcggttat tggcagacag ctgcgccagc tccagttcat caacgagcgc
     4261 cttaagacgt gcagagtcag actgcaacgc gaaattttgt gcctgattga gcggagtatt
     4321 gccggtaagc gttcccaggc ggcgctcgat ctcatttaac tgacggcggg cgtcggtttg
     4381 ctgttgcggc agttgattca gcgaatcggc aatctcgcgg gcgcgctcct gctcttgctg
     4441 ggcctgacgg cttttatcca gcaactggct gctgacctgg agaatttcct gattcagcgc
     4501 gtcggtagac attcccggcg acacgctgcg cggcttgtca cgcatgttgt ttaattgtgc
     4561 gcgcagagta gcggagagtt tcggataatt atcgataact tgctgatatt gtttgatgcg
     4621 ctcaagggaa ccttttcgtt cctcaagcgc atttaaggca gactgaagcg cctctacgac
     4681 ttccggctgt gcgggtttcg ccgcttttgc ctgctccagt tcctgagtga tttgtttgct
     4741 atcgggggcc gtcgcggcgt acgcccccca actgaggcac caggccatca gaaaagtgat
     4801 aatcaggcgc acgtcagcgt ttcctttgat ggattagacc tggtcttttt tgtcgtcaac
     4861 caatgggctg gcgtcgtgtt ctgcttcgat ctcttcagca ggaagcgggg caggttcagc
     4921 gtctggcgta acaaaggttt cggtagatac tgccagcggc tggcaaattt tcgtgacaga
     4981 caggctttcc agttgctcaa ccagattcac tttacccggt gcaaacaggt tgataacggt
     5041 ggaaccgagt ttaaagcgac ccatttcctg gcctttcagc agtgccacag aaccgtcgtt
     5101 ttccccggca ggccaggtcc agcgcttgat gataccttcg cgcggcggcg taatggtgcc
     5161 cgcccagacc gtctcaatgc tgccaacaat cgtcgctccg accagaatct gcgccattgg
     5221 gccaaattcg gtatcgaaaa ggcaaatcac gcgttcgtta cgggcaaaca gattcggcac
     5281 gttctgagcc gtgagatggt taacggagaa gagatcgccc ggcacgtaga tcatctcacg
     5341 cagaataccg ttgcacggca tgtgtacgcg gtggtagtca cgcggggaga ggtaagtggt
     5401 cacaaacgta ccgttgcgga acaggtccgc catcagatag ttgcctgcca gcagggcttc
     5461 gaggctgtag ttgtggcctt tggcttgcag gattttatct tcttcgattt tacccaactg
     5521 gctgataacg ccatcggcag gcatgaccag tacattcgga tcggtatcga ttgggcgtac
     5581 ttcgtcacgc agcggacgga caaagaattc gttaaaggtg cggtagctgg cggtgtccgg
     5641 cttttgcgcc tctttcatgt cgaccttgta gtatttaacg aacagatcga taaccagttt
     5701 tgtcaaccat cctgcccgct tgcttgcgcc ccaacccgcc aggcgagtaa gccatagttt
     5761 cggcagaatg tactgtagcg aaagtttaaa tgaatttaac aaggtagcct ccaggccatt
     5821 gttttgtcgt tcctgattcg gcctacatgc cggatcctga aaaaaagggg acgattctaa
     5881 cgacggttag cttaattgtc agtcatccgt atcagaaaag tttttacgcg tttttacctg
     5941 cgccatgctt tccagaatac ggtgatagtt ttcgaaacgg gtttccgcga ttttcccttc
     6001 ctcaaccgct tcccggatag cgcagcccgg atcggtatcg tgtttgcaat cgcgatattt
     6061 acacagacct aaatagtcat ggaattcgac aaagccctga gtgatttgtt ccggctccag
     6121 gtgccagagg ccgaactcac gcacaccagg ggaatcaatc acatcaccgc cgtgcgggaa
     6181 gtgatacagc cgagcggcgg tcgtggtgtg ctgaccaagc cccgagttgt cagagacatc
     6241 gttggtcagg atctcttttt gcagccccag caacgcgttc agcaggctgg atttgccgac
     6301 gccagactgc ccggcaaaaa tgctgatgcg cccggtcaac gcctcttcca gcggttttag
     6361 cccatcctga gtatggctgg aaaccatcaa tacgcgataa ccgatattgc ggtagatatc
     6421 catctgctcg ttgacgaacg ccatgccttc gtcgtccagc aggtctatct tgttgagcac
     6481 aataatcggc tcaatctgca aggtttcgca ggccaccagg taacggtcga taatattgag
     6541 cgacagctcc ggcaaaatgg cggagacaat gacaatctgg tcgatgttgg cggcaatagg
     6601 ttttacgccg tcgtagaaat ccggacgcgt caacaccgag gtacgctcat gcaccgcttc
     6661 cacgatccct ttgacattta cgccttccgc cgccggttta cccggacgcc agactacgcg
     6721 gtcgccggtt accagcgaac ggatggtacg gcgaatattg cagcggtgaa cgtcgccatc
     6781 ggcggattcc acatcagcgt gcataccaaa gcggctgatg acgatacctt catcaggctc
     6841 gccaaacaga ttgtcgtcgt agtcgggctt ctccttagac gttttaagac gacgctggtg
     6901 attggcgttc acgcggcgct gctggccttt ggagagttta tttttactca atcgtactga
     6961 ctcctggtcg cccctgatgg gcaaaacatc tatgatacac gcaattgtgg atcaatatag
     7021 tcactgtgaa tgggtggaaa atagcatgag tgccaatgaa aacaacctga tttggatcga
     7081 tcttgagatg accggtctgg atcccgagcg cgatcgcatt attgagattg ccacgctggt
     7141 gaccgatgcc aatctgaata ttctggcaga agggccgacc attgcagtac atcagtccga
     7201 tgaacagctg gcgctgatgg atgactggaa cgtgcgcacc cataccgcca gcgggctggt
     7261 agagcgcgtg aaagcgagca cgatgggcga tcgggaagct gaactggcaa cgctcgaatt
     7321 cttaaaacag tgggtgcctg cgggaaaatc gccgatttgc ggtaacagca tcggacagga
     7381 ccgtcgtttc ctgtttaaat acatgccgga gctggaagcc tacttccact accgttatct
     7441 cgatgtcagc accctgaaag agctggcgcg ccgctggaag ccggaaattc tggatggttt
     7501 taccaagcag gggacgcatc aggcaatgga tgatatccgt gaatcggtgg cggagctggc
     7561 ttactaccgc gagcatttta tcaagctgta aaatttttcc tggtcacgta agcgccggaa
     7621 tccacttgcg gcggcgtgat tttgacgcta aattgaacgt cttgctgata atttcagcac
     7681 ttgagataaa aacgcaaaaa aaactttttt ggggggttgc agagggaaag atttctcgta
     7741 taatgcgcct cccgtaacga cgcagaaatg cgaaaattac gaaagcaaaa ttaagtagta
     7801 cgcgggaata gctcagttgg tagagcacga ccttgccaag gtcggggtcg cgagttcgag
     7861 tctcgtttcc cgctccaaaa tttgaaaagt gctgcaaagc acagaccacc caatcgcagt
     7921 atttcgaatt gcgacaaggc ggcaactgaa cgaatccttg ggaacttaca aaagtatgtg
     7981 actaaggtga gtgaaggcag ccaaggcagt agcggttcga aagacgaaga ttaagcggga
     8041 atagctcagt tggtagagca c
//
LOCUS       DAZPEP010000174         8036 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZPEP010000174 DAZPEP010000000
VERSION     DAZPEP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8036
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.175"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            321..437
                     /locus_tag="ACHUT9_003716"
                     /pseudo
     CDS             321..437
                     /locus_tag="ACHUT9_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010904967.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUT9_003717"
     CDS             812..1054
                     /gene="yiiF"
                     /locus_tag="ACHUT9_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFR1350098.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUT9_003718"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="ACHUT9_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFR1350099.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUT9_003719"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="ACHUT9_003719"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFR1350100.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHUT9_003720"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="ACHUT9_003720"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFR1350101.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHUT9_003721"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="ACHUT9_003721"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFR1350102.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHUT9_003722"
     CDS             7101..7934
                     /gene="fdhD"
                     /locus_tag="ACHUT9_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFR1350103.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1864 a         2065 c         2087 g         2020 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcggta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat
     7981 ttcgtgaatt ttattatttt atttataaat aaaacgcgtg tagtgacgtt ttcttt
//
LOCUS       DAZPEP010000175         7986 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZPEP010000175 DAZPEP010000000
VERSION     DAZPEP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7986
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.176"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="ACHUT9_003723"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="ACHUT9_003723"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFR1350104.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="ACHUT9_003724"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="ACHUT9_003724"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFR1350105.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="ACHUT9_003725"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="ACHUT9_003725"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFR1350106.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="ACHUT9_003726"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="ACHUT9_003726"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFR1350107.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3010..4599
                     /gene="prfC"
                     /locus_tag="ACHUT9_003727"
     CDS             3010..4599
                     /gene="prfC"
                     /locus_tag="ACHUT9_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFR1350108.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4992..5597
                     /gene="osmY"
                     /locus_tag="ACHUT9_003728"
     CDS             4992..5597
                     /gene="osmY"
                     /locus_tag="ACHUT9_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFR1350109.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5724..5885
                     /locus_tag="ACHUT9_003729"
     CDS             5724..5885
                     /locus_tag="ACHUT9_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFR1350110.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6007..7080
                     /locus_tag="ACHUT9_003730"
     CDS             6007..7080
                     /locus_tag="ACHUT9_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFR1350111.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7077..7859
                     /locus_tag="ACHUT9_003731"
     CDS             7077..7859
                     /locus_tag="ACHUT9_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1350112.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
BASE COUNT         1903 a         2062 c         2170 g         1851 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt attcggcgag ataattaacc tttcttcgcc tggcgggtca taatggcgcg
      301 atacaccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tctcatccag
      361 cacgtccggg taaggcagga aggcgttcgc tacaattcgc agctcgccgc cgctattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc aggctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatctg tccccactgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc gtatcgcaat ctgcgacgtc
     1081 atccaccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacag atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctggaaca
     2041 tctgatcgac gaactggaaa aacgcggcgt ggcgacacta tggctggaag tccgtgcctc
     2101 aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gttttgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgcacgccgc tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgccaaaatc
     2581 ggcatcatca ccaacggctt tagcgccttg cagcaagtgc gtctggaacg cacgggcctg
     2641 cgtgattact tcgatttgct ggtgatttcc gaagaagttg gcgttgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacctgc
     2821 tggctgaatg cgcacaatcg cgagcaacca gaaggcatcg cgcccacctg gaccgtttct
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc acccggatgc cggtaagact accatcaccg agaaggtgct gctgttcgga
     3121 caggccattc agaccgccgg tacagtaaaa ggccgtggtt ccaaccagca cgctaagtcg
     3181 gactggatgg agatggaaaa gcagcgtggg atctccatta ctacgtctgt gatgcagttt
     3241 ccgtatcacg attgcctggt taacctgctc gacaccccgg ggcacgaaga cttctcggaa
     3301 gatacctatc gtaccctgac ggcggtggac tgctgcctga tggttatcga cgctgcaaaa
     3361 ggtgttgaag atcgtacccg taagctgatg gaagttaccc gtctgcgcga cacgccgatc
     3421 ctcaccttta tgaacaaact tgaccgtgat atccgcgacc cgatggaact gctcgatgaa
     3481 gttgagaacg agctgaaaat cggctgcgca ccgatcacct ggccgattgg ctgcggcaag
     3541 ctgtttaaag gcgtttacca cctttataaa gacgaaacct atctctatca gagcggtaaa
     3601 ggccacacga ttcaggaagt ccgtattgtt aaagggctga ataacccgga tctcgacgct
     3661 gcggttggtg aagatctggc acagcagcta cgtgacgaac tggaactggt gaaaggcgcg
     3721 tctaacgagt tcgacaaaga attgttcctt gcgggcgaaa tcactccggt attcttcggt
     3781 actgcgctgg gtaacttcgg cgttgatcat atgctggatg gcctggtgga gtgggctcct
     3841 gcgccgatgc cgcgtcagac tgatacccgt accgtagagg cgagcgaaga caaatttacc
     3901 ggcttcgtat ttaaaattca ggccaacatg gacccgaaac accgcgaccg cgtggcgttt
     3961 atgcgcgtag tatccggtaa atatgaaaaa ggcatgaagc tgcgccaggt gcgtactgcg
     4021 aaagatgtgg ttatctccga cgcgctgacc tttatggcgg gcgaccgttc gcacgttgaa
     4081 gaagcgtatc cgggcgatat cctcggcctg cacaaccacg gcactattca gatcggcgac
     4141 acctttaccc agggtgagat gatgaagttc accggtattc cgaacttcgc accagaactg
     4201 ttccgtcgta tccgcctgaa agatccgctg aagcaaaaac agctgctcaa agggctggta
     4261 cagctttccg aagagggcgc ggtgcaggtg ttccgtccga tctccaacaa cgatttgatc
     4321 gttggtgctg ttggtgtgct gcagtttgat gtggtggtat cgcgcctgaa gagcgaatac
     4381 agcgttgaag cagtatatga atcagtcaac gttgccactg cccgctgggt agaatgtgcg
     4441 gacgcgaaga aattcgaaga gttcaagcgt aagaacgaaa gccaactggc gcttgatggc
     4501 ggcgataacc tcgcttacat cgctaccagc atggtcaacc tgcgcctggc gcaggaacgt
     4561 tatcccgacg ttcagttcca ccagacccgc gagcattaat tcttgcctcc agggcgcggt
     4621 agccgctgcg ccctgtcaat ttcccttcct tattagccgc ttacggaatg ttcttaaaac
     4681 attcactttt gcttatgttt tcgctgatat cccgagcggt ttcaaaattg tgatctatat
     4741 ttaacaaagt aatgacattt ctgacggcgt taaataccgt tcaatgcgta gatatcagta
     4801 tctaaagccg tcgattgtca ttctaccgat attaataact gattcagagg ctgtaatggt
     4861 cgttattcat cacccatcgc ttttgtgatg gcgaccattg acttctgtag agggtgaagt
     4921 ctctccctat tcagcaatgc aacctcgtgt tgccaggctc aaattacgag caaacataca
     4981 ggaataaatc gatgactatg acaagactga agatttcgaa aactctgctg gctgtaatgt
     5041 tgacctctgc cgtcgcgacc ggctctgcct acgcggaaaa caacgcacag actaccaatg
     5101 aaagcgcagg gcaaaaagtc gatagctcta tgaataaagt cggtaatttc atggatgaca
     5161 gcgccatcac cgcgaaagtg aaggcggcgc tggtggatca tgacaacatc aagagcaccg
     5221 atatctctgt caaaaccgat caaaaagtcg tgaccctgag cggtttcgtt gaaagccagg
     5281 ctcaggccga agaggcagtg aaagtggcga aaggcgttga aggggtgacc tctgtcagcg
     5341 acaaactgca cgttcgcgac gctaaagaag gctctgtgaa gggctacgcg ggtgacaccg
     5401 ccaccaccag tgaaatcaaa gccaaactgc tggcggacga tatcgtccct tcccgtcatg
     5461 tgaaagttga aaccaccgac ggcgtggttc agctctccgg taccgtcgat tcccaggcac
     5521 agagcgaccg tgctgaaagt atcgccaaag cggtagatgg tgtgaaaagc gttaaaaatg
     5581 atctgaaaac taagtaattc gtcgtaattc gtcctcccga actttgtcgg gaggcgcgat
     5641 gtgcaccaca ctaaaaatat cgcattgagt gattttcacg ctcatatcaa gcggtgacat
     5701 taactatggt aaaggagacg cttatgtttc gttggggcat catatttctg gttatcgcgt
     5761 taatcgccgc cgcacttggg tttggtggtc tggccggtac cgctgcaggc gcagctaaaa
     5821 ttgtctttgt cgtcgggatt attctgttcc tggtgagttt gttcatgggc cgaaaacgac
     5881 cctagatttc agaccattaa gaaaatttta tccaaagcca gtccagcgga ctggcttttg
     5941 cggttttagc gaataataaa ttgcgttact ttgtcattct ttcacaacaa aaacaggaag
     6001 gcagaggtgg ggcagcgaat acctgtaacg cttggtaata ttgcgccgtt gtcgctaagg
     6061 ccgttccagc ctggacgaat agctctggtg tgcgaaggcg gcggacagcg tggaattttc
     6121 acggctggcg tgctggatga gtttatgcgc gcgcagttta atcctttcga tctttatctc
     6181 ggcacatctg ccggggcgca gaacctctcg gcgtttatct gcaatcagcc cggttacgcg
     6241 cgcaaagtca tcatgcgcta taccacaaaa cgcgaatttt tcgatccatt gcgctttgtc
     6301 cgtggaggaa atcttatcga tctcgactgg ctggtggagg ccaccgcaag ccagatgccg
     6361 ttgcaaatgg ataccgccgc gcggttgttt gacagcggca aatcgtttta tatgtgcgcc
     6421 tgtcgtcagg atgactacgc gcctaattac tttttaccaa ccaaacaaaa ctggctggat
     6481 gtgattcgcg cctccagtgc gatacctggc ttttatcgta gcggagtgtc gctggaaggc
     6541 attaactacc tggatggcgg gatcagtgat gcgattccgg ttaaagaggc ggcaaggcag
     6601 ggcgctaaaa cgttggtcgt cattcgcact gtgccgtcac aaatgtacta cacgccgcag
     6661 tggttcaaac gcatggaacg ctggctgggt gacagtagcc tgcagccgct ggtcaatctg
     6721 gtgcagcatc atgaaaccag ctatcgtgac attcagcaat ttattgagaa accaccgggc
     6781 aagctgcgga tattcgaaat ttatccgccg aagccattac atagtatcgc gcttggcagt
     6841 cggattccgg cgctgcgtga agactataaa cttgggcgtt tatgcggtcg ttatttcctc
     6901 gccacggttg gcaagctatt aactgaaaaa gcgccgctta cccgccatct ggtgccagtg
     6961 gtgacgccgg aatcgatcgt tattccgcct gcgccagtcg ccaacgatac gctggttgcc
     7021 gaagtgagcg atgctccgca ggcgaacgac ccgacattta acaatgagga tctggcttga
     7081 tttgccgttt tatcgatacc cactgccatt ttgatttccc gccgtttagt ggcgatgaag
     7141 aggccagcct gcaacgcgcg gcacaagcgg gcgtaggcaa gatcattgtt ccggcgacag
     7201 aggcggaaaa ttttgcccgc gtgcaggcgt tagcggaaaa atatcaaccg ctgtatgccg
     7261 cattggggtt gcatccgggg atgttggaaa aacatagcga tgtgtctctt gatcagctac
     7321 agcaggcgct ggaaaggcgt ccggcgaagg tggtggcggt gggggagatc ggtctggatc
     7381 tcttcggcga cgatccgcaa tttgagaggc agcagtggtt actcgacgaa cagctgaaac
     7441 tggcgaaacg ctacgatctg ccggtgatcc ttcattcccg gcgtatacac gacaaactgg
     7501 cgatgcatct taaacgtcac gatttaccgt gcactggcgt ggttcacggt ttttccggca
     7561 gcctgcaaca ggccgagcgg tttgtacagc tgggctacaa aatcggcgta ggcggtacta
     7621 tcacctatcc acgcgccagt aaaacccgcg atgtcatcgc aaaattaccg ctggcatcgt
     7681 tgttgctgga aaccgacgcg ccggatatgc cgctcaacgg ctttcagggg cagcctaacc
     7741 gaccggaaca ggctgtgcgg gtgtttgacg tgctttgcga gttgcgcccg gaaccagaag
     7801 atgagattgc cgaagtgttg cttaataata cgtatgcgtt gtttagcgtt agtgggtagc
     7861 ctacccttcg taggcagcat ccggcagtcg gcgcataatg cctgatgcga cgcttaacgc
     7921 gtcttatcat gcctacaggt ttgtgccgaa ccgtaggccg gataaggcgt tcacgccgca
     7981 tccggc
//
LOCUS       DAZPEP010000176         7951 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZPEP010000176 DAZPEP010000000
VERSION     DAZPEP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.177"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..365
                     /locus_tag="ACHUT9_003732"
     CDS             <1..365
                     /locus_tag="ACHUT9_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFR1350113.1"
                     /translation="ITGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERL
                     GERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFL
                     LRKDIMRRLTQDVCEEIE"
     gene            complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHUT9_003733"
     CDS             complement(422..2800)
                     /gene="ppsA"
                     /locus_tag="ACHUT9_003733"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFR1350114.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHUT9_003734"
     CDS             3133..3966
                     /gene="ppsR"
                     /locus_tag="ACHUT9_003734"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFR1350115.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4123..5169
                     /gene="aroH"
                     /locus_tag="ACHUT9_003735"
     CDS             4123..5169
                     /gene="aroH"
                     /locus_tag="ACHUT9_003735"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFR1350116.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            5301..5492
                     /gene="hemP"
                     /locus_tag="ACHUT9_003736"
     CDS             5301..5492
                     /gene="hemP"
                     /locus_tag="ACHUT9_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFR1350117.1"
                     /translation="MRYTDSRKLTPETDANHKAASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(5496..6932)
                     /gene="selO"
                     /locus_tag="ACHUT9_003737"
     CDS             complement(5496..6932)
                     /gene="selO"
                     /locus_tag="ACHUT9_003737"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFR1350118.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(6995..7708)
                     /gene="ydiV"
                     /locus_tag="ACHUT9_003738"
     CDS             complement(6995..7708)
                     /gene="ydiV"
                     /locus_tag="ACHUT9_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFR1350119.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT         2034 a         2086 c         1888 g         1943 t
ORIGIN      
        1 aaattaccgg acgcaaaaaa gatattattg tccgcggcgg cgaaaatatt agcagccgtg
       61 aagtagaaga tattttattg cagcatccta aaattcacga tgcctgtgtg gttgcaatgc
      121 ccgatgaacg tttaggtgaa cgatcatgcg cttatgtcgt gctgaaagcg ccgcatcatt
      181 cattatcgct ggaagaggtg gtggcttttt ttagccgtaa acgggtcgca aaatataaat
      241 atcctgaaca tatcgtggta atcgaaaaac taccgcgtac tgcctcaggt aaaatacaaa
      301 agtttttgtt aagaaaagat attatgcggc ggttaactca ggatgtctgt gaagagattg
      361 aataagtttc atcttcgggg atcacataac cccggcgact gaacgccgcc ggggatttat
      421 tttatttctt cagttcagcc aagcttaacc aggtttgcac cacggtgtcc gggttcagag
      481 acaggctatc gatcccctct tccatcaacc atgcggcaaa gtcttcgtgg tcggacggac
      541 cctgaccgca aatcccgaca tatttgccct gtttcttcgc ggcacggata gccatcgaca
      601 gcagtgcttt caccgcatcg ttgcgctcat cgaacaattc agacaccacg ccggagtcac
      661 ggtccagacc gagcgccagc tgcgtcatat cgtttgagcc aattgagaag ccgtcgaaat
      721 attcgaggaa ctgctcggcc agcaaggcgt tggacgggat ttcacacatc atgatgattt
      781 tcagcccgtt ctcaccacgc ttcagcccct gacgcgccag ttcctcaacc accgctttcg
      841 cctgatctac ggtacgcacg aacgggatca tgatctcaac gttggtcagc cccatgtcgt
      901 tgcgcacacg tttcactgct tcgcactcca gcgcgaagca gtcgcggaag ctgtcggaaa
      961 catagcgtcc cgcgccacgg aagccgagca tcgggttctc ttcatctggc tcgtaacgct
     1021 caccaccgac caggttggca tattcgttcg atttaaaatc agagagacgg acaatgacgc
     1081 gcttcggata aaacgcggca cccagcgtcg cgatcccttc agtcagacga ccaacgtaaa
     1141 attcacgcgg agaatcaaaa cctttcatca tctcgcggat ttcattttgc aactgcggtt
     1201 cctgatcgtc aaactcaagc agtgcgcgtg ggtggacgcc aatcatacgg ttgatgataa
     1261 attccagacg cgcaaggccc acgccttcgt tcggcaggca ggcgaagtcg aaagcacggt
     1321 ccgggttacc gacgttcatc atcactttca acggcagatc cggcatcatt tctacgctgg
     1381 agcttttcac gctaaattcc agcaactccg catagacgta accggtatca ccttcggcac
     1441 aagaaacagt gacgttctca ccgtctttca tccgttctgt tgcatcacca cagcccacta
     1501 ccgccggaat gcccagttca cgagcgatga tcgccgcgtg acaggtacga ccgccacggt
     1561 tggtgacgat ggcagatgct ttcttcatga tcggttccca gtccgggtcg gtcatgtcag
     1621 tgaccagcac gtcaccaggt tcgatgcggt tcatttcgct gatctcatgg atgactttca
     1681 ccggacccgc gccgatgcga tgaccgatag cacggccttc ggcgataatc ttaccctgtg
     1741 aatgcagcgt ataacgctcc atgacctgac cgcgtgagcg cacggtttcc ggacgcgcct
     1801 gcacaatgaa cagtttgcca gtgtggccat ctttcgccca ctcaatatcc atcgggcgac
     1861 cgtagtgttt ctcaatttgt acggcctgtt ttgccagttc ctgcacttct tcgttggtca
     1921 gcgagaagat gtcacgctgt tcctgcggta cgtcttcgat tttaacctgc ttgccgtgct
     1981 cctgggtcgg cgcgtaaacc atgcggattt ttttcgaccc catggtgcga cgcacgatag
     2041 ccgggcgatt cgccgccagt gtcggtttat ggacgtaaaa ctcatccggg ttaaccgcac
     2101 cctgcacgac catttcccca aggccccatg cggaagtgat aaacaccacc tggtcaaagc
     2161 cggattcggt atcaatggag aacatcacgc cagaagatgc gaggtcggag cgcaccatcc
     2221 gctgaacacc ggcagagagc gccacgccac ggtgatcgta accctggtgc acacgataag
     2281 agatggcgcg atcgttaaac agagaagcaa atacatgctt cactgccacg agaacggcgt
     2341 caaaaccctg aacgttgagg aaggtttcct gctgaccggc aaaagaagcg tccggcatat
     2401 cttctgcggt ggcggaggag cgcaccgcaa aagaggcgtt ttcgtcatcg gcggaaagct
     2461 gtgcataggc ttcgcggatg gcgttttcca gctcaggctg gaagggagtg tcgataatcc
     2521 actggcggat ttgcgcgccc gctttcgcaa gctgagtaac atcgtcaata tccgttttat
     2581 ccagcagttc ataaatgcgc tggtttacgc cgctttggtc cagaaactgg ttaaacgcgt
     2641 cggcggttgt ggcgaaacca ttcggaacgg aaacacccat tccggaaagg ttagtaatca
     2701 tttcacccag ggaggcattt ttgcccccaa ccctgtctac atcattcatg ccgagttggt
     2761 tataccaaag caccagcggt gacgagccat tgttggacat cgaacaatcc ttttgtgata
     2821 aatgaacggt ttgagaaaca catttctgcg catttatctt tgcatattta accggatgaa
     2881 aaaaacggtg aatcgttcaa gcaaatatat ttttttactt tttaagactg atcccagcgt
     2941 tgcgcaaatc tgctctcttc gacaatttcc acataaacca tcggtataaa cgaaaccata
     3001 aaaatgaaat gctgttttca taaaaaataa aattgaaggt tcattttata aaccagacat
     3061 aacgtcctac gcttctgtgc gtttttaatt tatgctttca tagaattatg tctgcatcac
     3121 gggaagaaca aaatggataa tgctgttgat cgccacgttt tttatatttc tgatggtacg
     3181 gcaataactg cggaggtatt aggacacgca gtaatgtcac aatttcccgt cactatcagc
     3241 agcattacgc tgccgtttgt cgaaaatgag agccgtgcac gggcagtgaa ggatcagatt
     3301 gacgcaattt atcaccagac aggtgtgcgc ccgctggtct tctactccat cgtgttgccg
     3361 gagattcgcg ccatcatctt gcaaagtgaa ggcttttgcc aggatatcgt tcaggcgctg
     3421 gttgccccgc tacaacaaga gatgaaactg gatccaacgc cgattgctca tcgtacccat
     3481 ggccttaacc ctaataatct caataaatat gatgcgcgca ttgcggcgat tgattacacc
     3541 ctcgcccacg atgacggcat ttcgttgcgc aatctggatc aggctcaggt gatcctgctc
     3601 ggtgtttctc gctgtggtaa aacccccacc agtctttatc tggcaatgca atttggtatc
     3661 cgcgcggcaa actacccctt tattgccgac gatatggata atctggtgct acccgcgtcg
     3721 ctcaaaccgc ttcagcataa attgttcggc ctgactatcg acccggaacg tctggcggcg
     3781 attcgcgagg aacgtcggga gaacagtcgc tatgcctcgc ttcgtcagtg caggatggaa
     3841 gtcgcggaag tggaagcgtt gtaccgtaaa aatcagatcc cgtggattaa cagtaccaat
     3901 tattcggtag aagagattgc caccaagatc ctcgatatca tgggccttag tcgccgaatg
     3961 tactagagaa ctagtgcatt agcttatttt tttgttatga tgctaaccac ccggcgaggt
     4021 gtgacacacc tcgcacttga aatcagcagc gattggttta tcgtgatgcg catcacttcc
     4081 cggcagtcct gccgtagaag caacaaattt ctgagacttg taatgaacag aactgacgaa
     4141 ctccgtactg cgcgtattga gagcctggta acgcccgccg aactcgcgct acggtatccc
     4201 gtaacgcctg gcgccgccac ccatgtcacc gactcccgcc gcagaattga aaaaatactc
     4261 aatggtgaag ataagcgact gttggtcatt attggcccct gctcgatcca cgatctcacc
     4321 gctgcaatgg agtacgcccc ccgtctgcag tcgctgcgca accagtacca gtcacggctg
     4381 gaaatcgtaa tgcgcaccta ttttgaaaaa ccacgaactg ttgtcggctg gaaaggacta
     4441 atctccgatc cagatttaaa cggcagctat cgggtaaatc acggtctgga gctggcgcgc
     4501 aaattacttt tacaggtaaa tgagctgggc gtcccaaccg cgactgagtt cctcgatatg
     4561 gtgaccggtc agtttattgc tgatttaatc agttggggcg cgattggcgc acgtactacc
     4621 gaaagtcaga tccaccgtga aatggcttcg gcactctcct gtccggtagg ttttaaaaat
     4681 ggtaccgatg gcaatacgcg gattgctgtg gatgctatcc gcgcagcccg cgccagccat
     4741 atgttcctct cgccagacaa aaatggtcag atgaccatct atcagaccag cggcaacccg
     4801 tatggccaca ttattatgcg tggcggcaaa aaaccgaatt atcatgccga tgatatcgcc
     4861 gcagcctgcg atacgctgca cgagtttgat ttacctgaac atctggtggt ggatttcagc
     4921 cacggtaact gccagaagca gcaccgtcgc cagttagaag tttgtgagga tatttgtcag
     4981 caaatccgca atggctctac ggcgattgct ggaattatgg cggaaagttt cctgctcgaa
     5041 ggaacgcaaa aaatcgtcgg cagtcagccg ctcacttacg gtcaatccat taccgacccg
     5101 tgtctgggct gggaagatac cgaacgcctg gtcgaaaaac tcgcctctgc ggtagatacc
     5161 cgcttctgaa tgcgtgccca ttcctgacgg aatgggcatt tctgcgcaac ttgttgtctt
     5221 ctcaacaaat tactgcttgc tctggcctgc cataatattg ataataagaa tcattgttat
     5281 atcaattatt attaatcttt atgcgttata cggatagcag aaaactcacg cctgaaacgg
     5341 acgccaatca caaggccgct tccccgcagc ccatccggcg aattaccagc cagacactgt
     5401 taggtccgga tggcaaactg attatcgatc atgacgggca agaatatctg ctccgtaaaa
     5461 ctcaggctgg caagctgctg ttaactaagt agcctttaac tcgagcagct gacttccagc
     5521 cgtttacccc agtcaggtgg acgactgaca tagtcatcat ctctatcgct gaaaggattt
     5581 cgcaacgccc catgcaggcg gtgcaattcc gtcatatcac ccttttctgc cgcttcaatc
     5641 gcccgttgcg ccaaccagtt gcgcaatacc agagcggggt taacgctttg catcaactgc
     5701 tgacgctcac tatcgctaac ctcgtcttgt tgcaaacgcc cccgataacg ggcaaaccag
     5761 ccatcaaatg ccgcacgatc aataaactca tcacgtagcg gtgacgccgt gctgtgctgc
     5821 tcggtcagac tcagcatgcg gaatgtgcgg gtataatcgc tgcgctctcg cgccatcaga
     5881 ctgaataatt cattcagtag cgcgttatcc tctttttgct ccgtgatgaa gcccagtttc
     5941 tgccgcatcc gttgtccata atgcgtcaac aaaacctgct gatagctgtc cagggcctca
     6001 ttcagggcat ctacggcaac aaatggtgac aatgtctgcg ccagacgctg taaattccac
     6061 aacgcgacag caggttgatt atcaaaacta taacgccctt gatgatccga gtgattacaa
     6121 ataaaaccgg gttcgtaatc atcaagaaaa ccaaacggcc cgtaatcaag cgtcagtccc
     6181 agcagcgaca tgttgtcggt attcatcacc ccatgtgcaa agccgaccgt ctgccattgg
     6241 gcaattaacg acgcggtacg tgcgacaaca tcgctaaacc agagacggta tttgtcctca
     6301 tcatcttcaa gatgtgacca gtaatgacga atagcaaagt cagctaactg acgaaccttt
     6361 tccgactcgc ggcggtagta aaaatgttcg aaatgaccaa agcgcagatg acttggtgcc
     6421 acacgcatca gcatcgcgcc tggctccgcc gtttcccgat acactggcga atcgctggtg
     6481 acgatactta acgcgcgggt cgtcggaatg cccagataat gcatcgcctc actggcgaga
     6541 ctttctcgta tcgtcgaacg taaaaccgcc cgtccatcac ccattcgcga ataaggcgtc
     6601 aggccagcac ctttcagatg ccagtccatt gtagtgccat cagcaagcag ttgttcgccg
     6661 agtaaaatgc cgcgcccatc acccagttgg cccgcccaga cgccgaactg atgaccactg
     6721 taaacctgcg ccagtggtga cataccaggc agtagggctt cgccgcccca gacacctgcg
     6781 ccatttttaa acagcgacga tggaatactc agcgtgttag ccagttcggt attatgccaa
     6841 atcagccggg cattatttaa aggcgtaggg gaaagtgctg tataggtttc tggcaattca
     6901 tcgcgccagc gggtaacaaa agacagggtc atagatcctc ctgtttgata gtgtagacgg
     6961 ttactctcgt cttaaacacc agcaaacaga agggttatcg ctgaaccaac gtcgttatct
     7021 gatttatggg aacggcaggc cacaggcatc cctggagagc atggaaatcg aagggggtaa
     7081 tctgtgccaa tatttctgcc gtatcaatgc ctcccgcaat gatgcaattg caacaagggg
     7141 aaatttgcgc ctggatcgca cgaataaacg gttcgaagga gcggtgcgtg atttgttgct
     7201 gaataaaact tttatccagc atgacgcggg tgaaaagacc atcaaaaacc gctttcattg
     7261 tgctattgcc tgctcccaaa ttacctaaca caaggggata cacttgcgat aaggataaaa
     7321 gaccgcgatt atcttttcct tcattgagat gcgggtaatt ctcattaatc aacagttcaa
     7381 taaacggata ttttaatagt tcgcctgcgt aattatccct ctctaataat aatgtagcca
     7441 cttgtggcgt taaattaagc caggcaaaga gtttatgttg aataaaaaaa tgttggcatg
     7501 attttagtaa ttccaactgc tctgaaaata attgccaatg ttgctcctcg gtaagttgcg
     7561 ctataacccg actcgtcggg atacgcaccg tgccatcttc actggagaaa tgggtaatta
     7621 actcaacgcc gacaaggact tgctggttat ctcttatcgg caggaaatag caatcagaat
     7681 gataaagatt ctccaaaaaa atcttcatgg taaccgtccc tctcgaagga tgtttttcag
     7741 tatccggcta aaagggatga ggccataaga cataacaatt acagaaggag taactttcat
     7801 ttgttccatg ttaaccactt tttcaggggt ccttttttta gattatcctg attataaacg
     7861 aataatccat ttacggaatt tttgtctgca aaatactact gtattcagga gtaaatggac
     7921 ggcgatgtat catcgccgtc catttttcga c
//
LOCUS       DAZPEP010000177         7945 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZPEP010000177 DAZPEP010000000
VERSION     DAZPEP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7945
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.178"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            97..186
                     /gene="kdpF"
                     /locus_tag="ACHUT9_003739"
     CDS             97..186
                     /gene="kdpF"
                     /locus_tag="ACHUT9_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFR1350120.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            186..1859
                     /gene="kdpA"
                     /locus_tag="ACHUT9_003740"
     CDS             186..1859
                     /gene="kdpA"
                     /locus_tag="ACHUT9_003740"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFR1350121.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLIALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1882..3930
                     /gene="kdpB"
                     /locus_tag="ACHUT9_003741"
     CDS             1882..3930
                     /gene="kdpB"
                     /locus_tag="ACHUT9_003741"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFR1350122.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3939..4511
                     /gene="kdpC"
                     /locus_tag="ACHUT9_003742"
     CDS             3939..4511
                     /gene="kdpC"
                     /locus_tag="ACHUT9_003742"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFR1350123.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4504..7188
                     /gene="kdpD"
                     /locus_tag="ACHUT9_003743"
     CDS             4504..7188
                     /gene="kdpD"
                     /locus_tag="ACHUT9_003743"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFR1350124.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7185..7862
                     /gene="kdpE"
                     /locus_tag="ACHUT9_003744"
     CDS             7185..7862
                     /gene="kdpE"
                     /locus_tag="ACHUT9_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFR1350125.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
BASE COUNT         1621 a         2085 c         2356 g         1883 t
ORIGIN      
        1 atactttttt tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca gaattctact
       61 cttccggtat cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc
      121 gtgttgctgg tgtttttatt actgggttat ttggtttatg ccctgatcaa tgcggaggcg
      181 ttctgatggc tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc
      241 tggcgcgtcc tttaggcagc gggctggcgc ggctgattaa tgacattcct cttccaggta
      301 cagcgggcgt tgaacgtata ctctttcgtt tacctggcgt ctctgaccat gagatgaact
      361 ggaagcaata tctttgtgcc attctcggcc tgaacatgct ggggctggcg gtgctgtttt
      421 ttatgttgct cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt
      481 gggatctggc gctgaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata
      541 gcggtgaaac cacgttgagc tatttcagcc agatggcagg cttaacggta caaaactttc
      601 tttctgccgc cagcgggatt gcggtgattt ttgccttcat ccgtgcgttt actcgccaga
      661 gcatgagcac actcgggaat gcctgggtcg atctgctacg catcacgtta tgggtgctag
      721 tccctgtggc gttgttgatc gcactgtttt ttattcaaca aggtgcgctg caaaactttc
      781 tgccctatca ggcagtgaat accgttgaag gagcgcaaca gctgttaccc atgggaccag
      841 tagcttctca ggaagcgatc aagatgctcg gtactaacgg cggtggcttc tttaatgcca
      901 actcgtcgca tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag atgctggcga
      961 tcttcttgat cccaacagcg ctgtgctttg cctttggtga agtgacgggc gatcgccgcc
     1021 aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt catctgcgta ggcgtggtta
     1081 tgtgggcaga agttcagggt aatcctcatc tgctggcgct cggcgcagac agcagcatca
     1141 atatggaagg taaagagagc cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga
     1201 ctacggcggc gtcctgtggc gcggtgattg cgatgcatga ttcgtttacc gctctcggcg
     1261 gcatggtgcc gatgtggcta atgcaaattg gtgaagtggt gttcggcggt gtcggttctg
     1321 gtctttacgg catgatgctg tttgtcctgc tggcggtgtt tattgccggg ctgatgattg
     1381 gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa ctgattgcac
     1441 tggcgattct ggttactcca acactggtac tgatgggcgc ggcgttggcg atgatgaccg
     1501 acgccggacg tagcgccatg cttaaccctg gcccgcatgg ttttagcgaa gtgctgtacg
     1561 ccgtgtcgtc cgccgctaac aacaacggca gcgcctttgc cggattaagc gccaactctc
     1621 cgttctggaa ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc
     1681 cggtgatggc aattgccggt tcgctggtga gtaaaaagag ccaacccgcc agctccggca
     1741 cgctgccaac gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg
     1801 gcgcactgac ctttatccct gccctggcgc ttggtccggt ggcggaatat ctctcctgat
     1861 gatattgagc gagcactgaa tatgagtcgt aaacaactgg cgctattcga accaacactt
     1921 gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat
     1981 ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca cctgtattag catcgcgatg
     2041 gcaagcggtg cgatgcctgg caatgcgctg tttagcgcgg caattagcgg ctggctgtgg
     2101 gtcaccgtac tgttcgctaa tttcgccgag gcgctggcag aaggccgcag taaagcgcag
     2161 gccaacagtc ttaaaggggt gaaaaaaact gcctttgccc gcaagctgcg tgagccgaaa
     2221 tatggcgctg cggcggacaa agttcctgcc gatcaactgc gtaaaggtga catcgtgctg
     2281 gtagaagctg gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg cgcatcggtc
     2341 gatgaaagcg ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt
     2401 gcctccgtca ccggcggcac gcgtattctt tctgactggc tggtgattga gtgtagcgtt
     2461 aaccccggtg agacatttct ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc
     2521 aaaacaccga acgagattgc cctgactatc ctgctgattg ctctgaccat cgtcttttta
     2581 ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg gtaatgccgt gagtgtaacg
     2641 gtactggtgg cgctgctggt ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg
     2701 atcggcgtcg ccgggatgag tcggatgctg ggcgcgaatg tgattgccac cagcggacgt
     2761 gcagttgaag cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg caccatcaca
     2821 ctcggtaacc gtcaggcgtc ggagtttatc cccgcgcagg gcgtggatga aaaaacgctg
     2881 gctgacgccg cacaactggc ttcgctggct gatgaaacgc cggaaggtcg cagtattgtg
     2941 atcctcgcca agcaacgttt taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc
     3001 tttgtaccgt ttactgcgca aagccggatg agcgggatca acatcgacaa ccgcatgatc
     3061 cgtaaaggtt ctgtcgatgc cattcgccgc catattgagg ctaacggcgg tcacttccct
     3121 gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg gagccacgcc gctggtggtg
     3181 gtggaaggtt ctcgtgtgct gggcgttatt gcgctgaaag atatcgtcaa aggcggtatt
     3241 aaagagcgct tcgcccagct gcgcaaaatg ggcattaaaa cggtgatgat taccggcgat
     3301 aaccgtctga ctgccgccgc gatagctgcg gaagcgggtg tcgatgattt tctcgccgaa
     3361 gcaacaccgg aggccaagct ggcattgatc cgtcagtatc aggcggaagg tcgtttggta
     3421 gcgatgaccg gcgacggcac caacgatgct ccggcgctgg cgcaggcaga tgtcgcggtg
     3481 gcgatgaact ccggcaccca ggcggcgaaa gaggcgggca atatggtgga tctcgactct
     3541 aacccgacca agttgatcga ggtggtgcac attggcaaac agatgctgat gacccgtggc
     3601 tcgctgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat tattccggcg
     3661 gcgttcgcgg caacatatcc gcagttaaat gcgctgaaca tcatgcgcct gcattcgccc
     3721 gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt tttgattcct
     3781 ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac
     3841 ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa agtcattgat
     3901 ttactgctga ccgtttgcgg tctggtgtga ggtttaccat gagtggatta cgtccggcat
     3961 tatcgacatt tctctttctg ttattgatta ctggcggcgt ttacccgctg ctgaccaccg
     4021 cactggggca atggtggttt ccctggcagg ccaacggttc gttgatccgt gaaggtgata
     4081 cggtgcgtgg ctcggcatta atcgggcaga attttaccgg caacggctat tttcatggtc
     4141 gcccgtcagc aacggctgaa atgccctata atccacaggc ttctggcggg agcaatctgg
     4201 cggtcagtaa ccctgagctg gataaactaa tagccgcacg cgttgctgca ttacgggccg
     4261 ctaacccgga tgccagcgcg agcattccgg ttgaactggt gacggcatcg gcaagcgggc
     4321 tggacaataa tatcaccccg caagcggcgg cctggcagat cccacgcatc gcgaaagcgc
     4381 gtaatctcag cgttgaacag ctcacgcagc tgatcgcaaa atacagccaa caaccgctgg
     4441 tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggcg ctggataagc
     4501 ttgatgaata acgaaccctt acgtcccgac cccgatcgtc tgctggaaca aactgccgcg
     4561 ccgcatcggg ggaagctgaa agttttcttc ggtgcctgtg caggcgtcgg gaagacctgg
     4621 gcgatgctgg cagaagccca gcgactgcgg gcgcaagggc tggatattgt ggttggcgtg
     4681 gtagaaaccc acgggcgaaa agataccgcc gcaatgctgg aagggctggc tgttctgccg
     4741 ccaaaacgcc tggcgtaccg tgggcggcac atctgcgagt ttgacctcga tgccgccctc
     4801 gcccgccgtc cggcgctgat cttaatggac gaactggcgc acagtaatgc gccaggttct
     4861 cgtcacccta aacgctggca ggacatcgaa gagctgctgg aagcaggcat tgatgttttc
     4921 actaccgtca acgttcagca tctggaaagt ctgaatgatg tggtcagcgg cgtcaccgga
     4981 attcaggtac gggaaaccgt gcccgatcct tttttcgatg ccgccgacga cgtggttctg
     5041 gtggacttgc ccccggacga tctgcgccag cggctgaaag aaggcaaagt ctatattgcc
     5101 gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gcaatctgat cgccctgcgc
     5161 gaactggcac tgcgccgtac tgccgatcgc gttgatgagc aaatgcgcgc ctggcggggg
     5221 catcctagcg aagagaaagt ctggcacacg cgcgacgcga tccttttatg catcggccat
     5281 aacaccggta gtgagaagct ggtccgcgcg gcggcgcggc tggcgtcacg gctgggtagc
     5341 gtctggcacg cggtgtatgt tgaaacccct gccctgcatc gcttaccgga gaaaaaacgt
     5401 cgtgcaattc tcagcgcctt acgtctggcg caggaactgg gcgcggagac ggcaacactt
     5461 tctgatccag cggaagagaa agcggtagtg cgttatgccc gtgaacataa tctcggcaag
     5521 attattctcg gtcgcccggc ctcgcgccgc tggtggcgtc gggaaacgtt tgctgaccga
     5581 ctggcgcgca tcgcccccga tctcgatcag gtgctggtcg cgcttgatga accacccgcc
     5641 cgcacgatta acaacgcgcc ggatagccgc tcttttaaag acaagtggcg ggtacagatt
     5701 cagggatgcg tggttgccgc cgcgttatgc gccgttatca ccttaattgc catgcagtgg
     5761 ctgatggcgt ttgatgccgc caacctggtg atgctgtatc tgcttggcgt ggtggtggtg
     5821 gcgctatttt atggacgctg gccttcggtg gttgccaccg tcattaatgt ggtgagtttc
     5881 gatctctttt ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca atatctgctg
     5941 accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc tggcgtgcgt
     6001 tatcaggcgc gggtagcccg ttaccgcgag caacgcacac ggcacttata tgaaatgtcg
     6061 aaagctctgg cggtgggccg cagtccgcag gacatcgccg ccaccagcga acaatttatt
     6121 gcctccacgt ttcatgcccg cagtcaggtg ttgttgcccg atgacaacgg taaattgcag
     6181 ccgttaacac atccgcaagg aatgacgcca tgggacgatg ccatcgcgca gtggagtttt
     6241 gataaaggcc tgcctgcggg cgcgggcacc gacacgttac ccggtgtacc gtaccagatt
     6301 ttgccgctaa aaagcggcga gaaaacctac gggctggtgg tggtggagcc ggggaatctg
     6361 cgccagttga tgatcccgga acagcagcgc ctgctggaga cgtttacgct gttagtcgcc
     6421 aatgcccttg agcggctgac gctaaccgcc agcgaagaac aggcgcggat ggcaagcgaa
     6481 cgtgaacaga tccgcaacgc cctgctggca gcgctttcgc atgatttacg cacgccgctt
     6541 acggtgctgt ttggtcaggc agaaatctta acgctcgatc tggcaagcga aggatcaccc
     6601 cacgcccgcc aggccagcga gattcgtcag catgtgctga acactacccg actggtgaat
     6661 aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa agagtggtta
     6721 acgctggaag aagtagtcgg cagcgcgctg caaatgctgg aaccgggttt atcgtcgccc
     6781 atcaatcttt ctctgccaga accgctgacc ttaatccacg ttgacgggcc actctttgaa
     6841 cgggtgctga ttaatctgct ggagaacgcg gtgaaatatg cgggtgcgca ggccgaaatt
     6901 ggtatcgatg cccactttga gggcgaaaat ctacaactgg atgtctggga taacggcccc
     6961 ggtcttccgc caggccagga gcagacgata tttgataagt ttgctcgcgg gaataaagag
     7021 tcggcggtgc cgggtgtcgg gcttgggttg gcaatttgtc gggcgattgt ggatgtacac
     7081 ggcggcacca ttaccgcgtt caaccgaccg gaaggtggtg cctgttttcg tgttacactt
     7141 ccccagcaaa ccgctcctga acttgaagac tttcatgagg atatgtgaca aacgttctga
     7201 ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac ggcgctggag ggcgacggga
     7261 tgcgcgtctt tgaggccgaa actctgcaac gcggcttgct ggaagcggca acccgtaagc
     7321 cagatttgat tattctcgat ctcggcctgc ccgatggtga tgggattgag tttatccgcg
     7381 acctgcgcca gtggagcccg gtgccggtga ttgtgctttc cgcacgcagc gaagagagcg
     7441 acaagatcgc cgcgctggat gccggagcgg atgattatct gagtaagccg tttggcattg
     7501 gcgaattgca ggcccgtctg cgcgtcgctt tacgccgcca ctccgccacc gccgcgcccg
     7561 atccgctggt gaaattttcc gatgttaccg tcgatttagc cgcccgcgtg attcaccggg
     7621 gtgaggaaga ggtgcatctc acaccaattg agttccgcct gctggcggtg ctgctcaaca
     7681 atgccggaaa agtactcacc cagcgccagc tccttaacca ggtgtggggg ccaaacgcgg
     7741 tcgaacacag tcactatttg cgtatttata tgggacatct gcgacaaaaa ctggaacagg
     7801 atccggcccg gcctcgccac ttcattactg aaaccggtat tggatatcgg tttatgcttt
     7861 gaattgttat tcaataaatt cgcttaaata gtgattaatt tattattaaa cctgcgccga
     7921 atattattat ttggcaggtt taatt
//
LOCUS       DAZPEP010000178         7830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZPEP010000178 DAZPEP010000000
VERSION     DAZPEP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.179"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUT9_003745"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="ACHUT9_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFR1350126.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="ACHUT9_003746"
     tRNA            1230..1305
                     /locus_tag="ACHUT9_003746"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHUT9_003747"
     CDS             complement(1361..2296)
                     /gene="ldtA"
                     /locus_tag="ACHUT9_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1350127.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHUT9_003748"
     CDS             complement(2358..3437)
                     /gene="cobT"
                     /locus_tag="ACHUT9_003748"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFR1350128.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHUT9_003749"
     CDS             complement(3449..4192)
                     /gene="cobS"
                     /locus_tag="ACHUT9_003749"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFR1350129.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHUT9_003750"
     CDS             complement(4189..4734)
                     /gene="cobU"
                     /locus_tag="ACHUT9_003750"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFR1350130.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4868..5305)
                     /locus_tag="ACHUT9_003751"
     CDS             complement(4868..5305)
                     /locus_tag="ACHUT9_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFR1350131.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5523..5698)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5787..6463)
                     /locus_tag="ACHUT9_003752"
                     /pseudo
     CDS             complement(5787..6463)
                     /locus_tag="ACHUT9_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHUT9_003753"
     CDS             complement(6666..7475)
                     /gene="pduF"
                     /locus_tag="ACHUT9_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HFR1350132.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT         2195 a         2079 c         1665 g         1891 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     2101 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     2161 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     2221 gataagttac cgccaggcta ttatggctgg caaaaagcag agcaaatgag caaaaaatat
     2281 ttacacgacg catcatgtcc ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt
     2341 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     2401 ttactggcag caagttcgcc catgttgtta tatatcgcac aggcagcttc gatgatgggc
     2461 atcgccagag ctgcgccact cccctcacct aaacgcatct ccatattgag ataaggctcc
     2521 agccccaaat gcgacagcgc aatacgcgcc cctttttctg ccgacaggtg agaaggaatg
     2581 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcggcagc ataagaaaga
     2641 aatccatcca gcaacacggg taaaccgcag gaagcagcgc ctaacatcac gccagccatt
     2701 ccgaccaaat caaatccacc cacttttgcc aggacatcaa cgccatcctg cggatttggt
     2761 tgattcaacg taatcgcccg gcgcacaaca tcaattttat tagccagttt atctgtcggc
     2821 aggtttgcgc caatcccaac cacttcttca ggagcccggc cagtgattgt gctgactatt
     2881 gccgctgccg gtgtcgtgtt tgccatcccc aggtcaccta caccaaacag cgtaacaccg
     2941 ttttttgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc tgccagatgg
     3001 cgactcattg ccggagctga agcaatattg ccactacctc gagcaacacg catgttgata
     3061 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtagac gttagcgccc
     3121 gcttgtgctg ccagcacaca cacgccggtt gttccgcggg tcatattttc agcctgaatg
     3181 gctgtcactt cttttgggga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     3241 atgaccagta ccgctttttt gcccacatgc ggaacgccat tcaaccccgg cattcctgcc
     3301 agttgtatgg caagcgcctc cagccttccc aggctaccaa caggtttgag taacccgtcg
     3361 atatgccgtt gtgcacgcga catagcggca ggatcaatag cagggatcgt attcagtaaa
     3421 tcggcaagtg tttgcatgtc acgtctcttt atagcagtgc cagcaggaag accagttcac
     3481 caagctctat agcagctccc agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa
     3541 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     3601 ccggcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     3661 catcaatttt gccaatgaac acattgccaa gtccttcttc ccgggcataa cgatgacgat
     3721 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
     3781 tcggttcgcc acgcagagcc aactcgctta ataccagaat ctttgccagt acaacaaaaa
     3841 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
     3901 cgcggctacg tgcagaaaat acgccatcac aggtatcggc gaggccgtcc aggtggaatc
     3961 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt acgccacacc
     4021 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaattaaag
     4081 gaaaagtaat aataccgcga gaataatgct cgaaatccag tccctgggac cagcgacgcg
     4141 gtacaggcag acgcgtaata aaagagagca tcgcccaaaa taatttactc atttgatttt
     4201 tactccaata cccgaaacca ccaaccatac ttcatttgcc gcagcggcca gctgctgatt
     4261 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
     4321 tcccacttcg ttagtcacta atacaacctt tgcgggacaa cgttggcagg cagcaatcaa
     4381 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
     4441 tttatcgccg ccataatcaa acaacagatt agtcaccatg gtggtaacgc attccagcaa
     4501 tacagcctca tgcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
     4561 cactgtgtgc cagtgcgccg ggcggccttg ccgatgatgt tctatccgtg cagccatctc
     4621 atcatcaagg atttgtgagg tggcgatata cagaacctgc gaagagtccc caataagcgc
     4681 ctctgcatgg cgactcttcc cgctccgtgc gccccccgtc accaaaatca tcataccgac
     4741 tcctgattca attgtatgat ggtattcatt gaacagaaca atggatcccc caccgttgct
     4801 gaccgcaaaa catttacgcg attaatactg cgcgtaatat aattaaaaat ccagcattct
     4861 caatccatta cgaaagaaga tggcattgcg gaatagccag acttcctcgt gttaacgtcc
     4921 gacaaaatgc gtcattgaca gcggtttctc tggaatgaca gatttcattc agataatgga
     4981 ttaatgctac agccccacca gccggaccag tcagcaaacg ggcattgtgt tctgcaatca
     5041 aaattaccgt cgttccctca gaatgagcga gccattctgg tacggatatc tcgctttcag
     5101 ctgctatcaa tagcagcact gaataatcgc tatacgcatc tgataacgat tgcacaggcc
     5161 caccatcaag aggctggaaa tcttgctgcc agtcctcacc aacaaaacag gttattggca
     5221 atatttcagc cagatgtttc gccagatcca catctccaga taagaggcaa aaaagagcta
     5281 tcgattcggg ttttatcgtt ttcatgcctc tcgccctcca cttaatttcc tgtaatggcg
     5341 gtctggttga taagccatga aacaatattg ccacaccttg aatgcagccc tggtgcctgt
     5401 cggcactgcg atgaaagaac taaatgatgc cgccataccc tgatcctttc agggtggtag
     5461 gggtataaag acgcgccagt actctaccgg acgcttccgg gcaacccact tcccaccgaa
     5521 gttgttggta ttgactgaca gactggtctt ctggcttagc gtcatcctca cccgaccttc
     5581 ccaatctcgc gatcagtggc attttcgggt ttgtcagcat tacagcagcg ggggctgcga
     5641 gggattttca ccctcttccc aggcacaaat gtgccacacc tgtcagcgta tgttatgagc
     5701 tgatttcact tttcccataa cacgtaaata acgttaatta ttcattccag cgtttattta
     5761 aaccttccct ttttttagca tttattctat accgtttcca tcgccgcatt ttcactcact
     5821 tgctgtcgat acacctgcgg cgtaacctga taagtctgac ggaaaacttt gcaaaaataa
     5881 ctgtattgtc gtggggatta ggcgcacgag tctggcgcac cgaatctgac cgagatttgt
     5941 tctctttaat aaccacaaaa ttgagttgtt ttttgaggca attttccaca atcaacttca
     6001 acaaatcagc agaggccata actcttgagt aatccatctc aggaactttg cggaattcat
     6061 taagtaattc gggatctgcc tgccagcaat catcgacatt cagaatatcg accaaatcga
     6121 catcatcatt acgcagacga acttgcccgc acaatacaaa accgactaaa tgtccggcga
     6181 ttaccagtgg aatagagaaa tcggttaatc cggcatgaca gcgataaata caaggctgat
     6241 cggatttaga ggcttccagg cccccacaac gatcgctcat gcgacatcga gtgcttagct
     6301 gagggtgttg ccgcatcatc tgacaaaatg gagtgaagtt aaacagctcg gaaatttcat
     6361 cgccgtgaat attgaccacg acaaccgcaa ggctggtcgc ctgcgcaaaa tcctgcgcga
     6421 ttttattaat gagttctgaa ttaagtgtcg ccgcagaaat catgataaaa cctcccagtt
     6481 aatttattgt tatagcaaaa caattgtttt acatttaatc ttcacatcat ccctgatgat
     6541 ttactgacga gaagcacaaa ggacaataac aaaagcctcc gtgaagcggg agacttttta
     6601 ataagttcgg aaagcagatc acaaacagta aaaaaataaa tgtttataac tgccagagta
     6661 tttcgttatt cgctaattcc tttaggtgta acactattga caggtaaatt ctttgcaatg
     6721 agatagcgat aaatcgccgc accaacacat gccccaatga tcggtgcaat aatgggtaca
     6781 atgaaataag gaatattgcg tccaccagtc atcgcaattt ctccccaccc cgcaagccag
     6841 gtgaaaatct taggtccaaa atcacgcgct ggattcatgg caaaaccggt taatggtccg
     6901 gtagaagcac caataacagc aaccagaata ccaataagta aaggtgcaag cggtcctttg
     6961 ggtacgccat taccgtcatc ggtcaacgcc ataatcaacc ccattaacat ggacgttatg
     7021 accacttcga ccaacgcagc ttgccataca ttaatcgccg ctgccggata agtactgaag
     7081 atactggcga gttgtaaact ttctgcgcta ccacgcacca tattgtgagc agattcaaaa
     7141 tcggtaaaca gactactgta tagagaatat gccagaactg caccaccaaa ggcacccgct
     7201 aattgcgcca gacaaaatgg cagcacttta cgggctggga aagaggcaaa cagccacaga
     7261 gcaatggtaa ctgccgggtt gagatgagcg ccggaaatac ctgccgtaag gtaaacagca
     7321 agcgaaatcc ctaaccccca aatgatacaa atttcccaca gtcccagact tgccccggca
     7381 actttcaggg cgcacaaaca accaatgcca aaaaagagga acagtccggt gcccaaaaac
     7441 tcggcaacgc attgcgcctt gagtgaatca ttcatagtga cacctttttt acggagtttt
     7501 tctttatcag ccaactatgc gctgactgtt gtatataccg agcacaacta taaaaaattg
     7561 ctctgcagaa aatattgcca acatgcatga cagactcgtg taaaaatcgt gctaacataa
     7621 caaattattg tcattaccga atcatgcaac acagcgttag tcagtgttga aagtaataat
     7681 tcccaccaaa cttttacaat aaaaaattgc catcatgaca taataaaatt gaaacaaata
     7741 tcacagttcc ttcttcgaac taaactcaac caaatattac tcgcgattaa actcactagc
     7801 caacgcaatt aattcacatt gaaatatcca
//
LOCUS       DAZPEP010000179         7774 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZPEP010000179 DAZPEP010000000
VERSION     DAZPEP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.180"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1780)
                     /locus_tag="ACHUT9_003754"
     CDS             complement(<1..1780)
                     /locus_tag="ACHUT9_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HFR1350133.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUT9_003755"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="ACHUT9_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFR1350134.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUT9_003756"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="ACHUT9_003756"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFR1350135.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUT9_003757"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="ACHUT9_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFR1350136.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUT9_003758"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="ACHUT9_003758"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFR1350137.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="ACHUT9_003759"
     CDS             complement(5968..7005)
                     /locus_tag="ACHUT9_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFR1350138.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHUT9_003760"
     CDS             7339..>7774
                     /gene="yhhY"
                     /locus_tag="ACHUT9_003760"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFR1350139.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKY"
BASE COUNT         1976 a         2102 c         1872 g         1824 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ctgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cgcgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt acatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacg
//
LOCUS       DAZPEP010000180         7759 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZPEP010000180 DAZPEP010000000
VERSION     DAZPEP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7759
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.181"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            223..726
                     /gene="dps"
                     /locus_tag="ACHUT9_003761"
                     /gene_synonym="pexB"
     CDS             223..726
                     /gene="dps"
                     /locus_tag="ACHUT9_003761"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFR1350140.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1130..1876
                     /gene="glnH"
                     /locus_tag="ACHUT9_003762"
     CDS             1130..1876
                     /gene="glnH"
                     /locus_tag="ACHUT9_003762"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFR1350141.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2015..2674
                     /gene="glnP"
                     /locus_tag="ACHUT9_003763"
     CDS             2015..2674
                     /gene="glnP"
                     /locus_tag="ACHUT9_003763"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFR1350142.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2671..3393
                     /gene="glnQ"
                     /locus_tag="ACHUT9_003764"
     CDS             2671..3393
                     /gene="glnQ"
                     /locus_tag="ACHUT9_003764"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFR1350143.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3510..5735
                     /gene="ybiO"
                     /locus_tag="ACHUT9_003765"
     CDS             3510..5735
                     /gene="ybiO"
                     /locus_tag="ACHUT9_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFR1350144.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5732..6658)
                     /gene="rlmF"
                     /locus_tag="ACHUT9_003766"
     CDS             complement(5732..6658)
                     /gene="rlmF"
                     /locus_tag="ACHUT9_003766"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFR1350145.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6934..7194
                     /gene="mcbA"
                     /locus_tag="ACHUT9_003767"
     CDS             6934..7194
                     /gene="mcbA"
                     /locus_tag="ACHUT9_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFR1350146.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7459..>7759
                     /locus_tag="ACHUT9_003768"
     CDS             7459..>7759
                     /locus_tag="ACHUT9_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFR1350147.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1887 a         1977 c         1946 g         1949 t
ORIGIN      
        1 gaattatgca aaagcatatt tattccgaaa attcctggcg agcagataaa taagaattgt
       61 tcttatcaat atatctaact cattgaatct ttattagttt tgtttttcac gcttgttacc
      121 actattagtg tgataggaac agccagaata gcggaacaca tagccggtgc tatacttagt
      181 ctcgttaatt actgggacat aacatcaaga ggatatgaaa ttatgagtac cgctaaatta
      241 gttaaatcaa aagcgaccaa tctgctttat acccgcaacg atgtctccga cagcgagaaa
      301 aaagcaacag tagagttgct gaatcgccag gttatccagt ttattgatct ttctttgatt
      361 accaaacaag cgcactggaa catgcgcggc gctaacttca ttgccgtaca tgaaatgctg
      421 gatggcttcc gcaccgcact gatcgatcat ctggatacca tggcagaacg tgcagtgcag
      481 ctgggcggtg tagctctggg gaccactcaa gttatcaaca gcaaaacccc gctgaaaagt
      541 tacccgctgg acatccacaa cgttcaggat cacctgaaag aactggctga ccgttacgca
      601 atcgtcgcta atgacgtacg caaagcgatt ggcgaagcga aagatgacga caccgcagat
      661 atcctgaccg ccgcgtctcg cgacctggat aaattcctgt ggtttatcga gtctaacatc
      721 gaataaatcc atcgctgatg gtgcagaact ttagtacccg ataaaagcgg cttcctgaca
      781 ggaggccgtt ttgttttgca gcccacctca acgcacttat ttagtgcatc catctgctat
      841 ctccagctga ttaagtaaat tttttgtatc cacatcatca cacaatcgtt acataaagat
      901 tgttttttca tcaggtttta cgctaaataa tcactgtgtt gagtgcacaa ttttagcgca
      961 ccagattggt gccccagaat ggtgcatctt cagggtattg ccctataaat cgtgcatcac
     1021 gtttttgccg catctcgaaa aatcaaggag ttgcaaaact ggcacgattt tttcatatat
     1081 gtgaatgtca cgcaggggat cgtcccgtgg atagaaaaaa ggaaatgcta tgaagtctgt
     1141 attaaaagtt tcactggctg cactgaccct ggcttttgcg gtttcttctc atgccgcgga
     1201 taaaaaatta gttgtcgcga cggataccgc cttcgttccg tttgaattta aacagggcga
     1261 taaatatgtg ggctttgacg ttgatctgtg ggctgccatc gctaaagagc tgaagctgga
     1321 ttacgaactg aagccgatgg atttcagtgg gatcattccg gcactgcaaa ccaaaaacgt
     1381 cgatctggcg ctggcgggca ttaccatcac cgacgagcgt aaaaaagcga tcgatttctc
     1441 tgacggctac tacaaaagcg gcctgttagt gatggtgaaa gctaacaata acgatgtgaa
     1501 aagcgtgaaa gatctcgacg ggaaagtggt tgctgttaag agcggtactg gctccgttga
     1561 ttacgcgaaa gcaaacatca aaactaaaga tctgcgtcag ttcccgaaca tcgataacgc
     1621 ctatatggaa ctgggcacca accgcgcaga cgccgttctg cacgatacgc caaacattct
     1681 gtacttcatc aaaaccgccg gtaacggtca gttcaaagcg gtaggtgact ctctggaagc
     1741 gcagcaatac ggtattgcgt tcccgaaagg tagcgacgag ctgcgtgaca aagtcaacgg
     1801 cgcgttgaaa accctgcgcg agaacggaac ttacaacgaa atctacaaaa aatggttcgg
     1861 tactgaaccg aaataataac gctacacctg taaagcgcac tggcagttcc ctctcccctc
     1921 tggggagagg attagggtga ggggcgcaaa cccgctccgg ggccattaat taccctgaat
     1981 ttgattattt acaccacggt aacaggaaca acatatgcag tttgactgga gtgccatctg
     2041 gcctgccatt ccgcttctga ttgaaggtgc caaaatgacc ctgtggattt cggtcctcgg
     2101 tctggctggc ggtctggtaa tcggattgct ggcaggtttt gcacgcacct tcggtggttg
     2161 gatagccaac cacgtcgcgc tggtctttat tgaggtgatc cgcggcacac ctatcgtcgt
     2221 ccaggtgatg tttatttatt tcgccctgcc gatggcgttt aacgacttac gcatcgaccc
     2281 atttactgcg gcggtggtca ccatcatgat caactccggc gcgtacattg cggaaatcac
     2341 gcgtggtgcg gtgctgtcta ttcacaaagg ttttcgtgaa gcaggactgg cgctcggtct
     2401 ttcacgttgg gaaaccattc gctacgtcat tttaccgctg gcactgcgtc gtatgctgcc
     2461 gccgctgggt aaccagtgga tcatcagcat taaagacacc tcgctgttta ttgtgatcgg
     2521 cgtggcggaa ctgacccgtc aggggcaaga aattattgcc ggtaacttcc gcgcccttga
     2581 gatctggagc gccgtggcgg tgttctatct gattattacc ctggtgctaa gctttatttt
     2641 gcgtcgtctg gaaagaagga tgaaaatcct gtgattgaat ttaaaaacgt ctccaagcac
     2701 tttggcccaa cccaggtgct tcacaatatc gatttgaaca ttgcccaggg cgaagtcgtg
     2761 gtgattatcg ggccgtccgg ttccggtaaa tcgaccctgc tgcgctgcat caacaaactg
     2821 gaagaaatca cctccggcga tctgattgtc gatggcctga aggttaacga tccgaaagtt
     2881 gacgagcgcc tgattcgcca ggaagcaggt atggtgttcc agcagtttta cctcttcccg
     2941 catctgacgg cgctggaaaa cgtcatgttt ggtccgctac gcgtgcgtgg cgcgaacaaa
     3001 gaagaggcgg aaaaactggc gcgtgagctg ctggcgaaag tcggtctggc agaacgtgca
     3061 catcactacc cttccgaact ttctggtggt caacagcagc gtgtggcgat tgcccgcgcg
     3121 ctggcggtga agccgaaaat gatgctgttt gatgaaccga cttccgctct tgacccggaa
     3181 ctgcgccatg aagtgctgaa ggttatgcag gatctggctg aagaagggat gacgatggtg
     3241 atcgtgaccc acgaaatcgg ttttgccgag aaagtagctt cgcggctgat ctttatcgac
     3301 aaaggccgga ttgcggaaga tggcgatccg caggtattga tcaagaaccc gccgagccag
     3361 cgcttgcagg aatttttgca gcacgtctct taacttctct gcctcccttt cacccgaaag
     3421 ggaggcacac cagattcctc tcatttaaaa tcgcccctcc cccagcatct atacttatct
     3481 ttttgctctg ttttctcact ggaggagtca tgcggtggat cctgttcatc ctcttctgcc
     3541 tgctgggcgc acctgcccac gcggtatcca tacccggcgt tacaaccaca acgacaacgg
     3601 actcaacgac tgaaccggcc ccggaaccgg atatcgaaca aaaaaaagcg gcctatggcg
     3661 cactggcgga tgtgctggat aacgacacct cgcgtaaaga attgatcgac cagttgcgta
     3721 ctgtcgccgc gacgccccct gcggaaccgg tcccgaagat cgtgccgccg acgctggttg
     3781 aagagcaaac cgtgctgcaa aaggtcaccg aagtcagccg ccattatggt gaagcccttt
     3841 ccgcccgctt cgggcaactt tatcgcaata tcaccggctc cccgcataag ccatttaatc
     3901 cacaaacctt cagcaatgcg ctgagccatt tttcaatgtt agcggtatta gtgtttggtt
     3961 tttactggct gattcgcctg tgcgcactgc cgctgtatcg caaaatgggt cagtgggcga
     4021 ggcaaaaaaa tcgtgagcgc agcaactggt tgcagcttcc ggcgatgatt atcggggcgt
     4081 ttattatcga cctgctgtta ctggccctga cattatttgt cggccaggta ttaagcgaca
     4141 acctgaatgc aggcagtcgc accatcgctt tccaacaaag tttgtttctc aacgcctttg
     4201 ccctgattga atttttcaaa gccgtactac gcctgatttt ttgcccaaac gtggcggagc
     4261 tgcgcccgtt cacgattcag gacgagagcg cccgttactg gagtcgtcgc ctgagctggt
     4321 taagcagttt gataggttat ggcctgattg tggccgtgcc gattatctct aatcaggtga
     4381 atgtacagat aggtgcgctg gcgaacgtca tcattatgct gtgcatgacc gtctgggcgt
     4441 tgtacctgat ctttcgtaat aaaaaagaga ttacccagca tttgctcaac tttgcggagc
     4501 attcgctggc ctttttcagc ctgtttatcc gcgcctttgc gctggtgtgg cactggctgg
     4561 caagcgccta ttttatcgtg ctgtttttct tttcgttgtt cgatccgggc aacagcctga
     4621 aatttatgat gggtgcaacg gtgcgcagcc tggcgattat tggtatcgca gcgtttgttt
     4681 ccggtatgtt ttcccgctgg ctggcaaaaa ccatcactct ctcgccacat actcagcgta
     4741 actatccaga gttgcaaaaa cggctgaatg gctggctttc ggcggcgctg aaaacggcgc
     4801 gtattctgac cgtctgcgtg gcggtaatgt tgctgttgag cgcatgggga ttgttcgatt
     4861 tctggaactg gctgcaaaac ggcgcggggc agaaaaccgt agatatcctg atccgtatcg
     4921 cacttattct ttttttctcg gcggttggct ggacggtgct cgccagtttg atcgaaaacc
     4981 ggctggcttc ggatattcat ggccgcccgc tacccagcgc ccgtacgcgt accctgctga
     5041 cgctgttccg taacgcgctg gcggtgatta tcagtaccat caccatcatg attgtgttgt
     5101 cggaaatcgg cgtcaatatc gcgccattgc tggcaggtgc cggggcatta ggtctggcta
     5161 tctcgtttgg ttcgcaaacg ctggtgaaag atatcattac cggggtgttt attcagtttg
     5221 aaaacggcat gaacactgga gatttggtga ctatcgggcc gttgaccggc acagtggaac
     5281 ggatgtcgat tcgctccgtg ggcgtgcgcc aggataccgg ggcgtatcac atcattccgt
     5341 ggtcttcgat aaccaccttt gctaacttcg tccgcggtat tggttcggta gtggcaaact
     5401 acgatgttga tcgccatgaa gatgctgata aagccaatca ggcactgaaa gatgcggtag
     5461 cggaattaat ggaaaacgaa gaaattcgcg ggctgattat tggtgaaccg aattttgccg
     5521 ggattgtcgg cttaagcaat accgcgttta cactgcgtgt ttcgttcacc acgctgccac
     5581 tcaaacagtg gacggtacgc tttgccctcg acagccaggt gaaaaaacat ttcgacctgg
     5641 cgggcgttcg cgcgccagtg cagacttatc aggtgctgcc tactccgggc gcgaccccgg
     5701 ctgaaccgtt accgccgggg gagccaacgc tttaacgctg gcgattgaca aaacggcggc
     5761 gctgctcgtc gttcataaag gtccaggcaa taaagcgact ctgcttttgc ccctgggcca
     5821 tctctttttt taccaccttc accgcgccca cgtcggtcag agcacgatac aacggcggta
     5881 agttttcacc acgagatacc agcgaggtaa accacatcac ctgcttcgcg aagcctttgc
     5941 tctcttcaat catctttttg ataaaggcga cttcaccacc ttcacaccac aactcctgtt
     6001 gctgaccacc aaagttcagt gcatcgtctt tgttcagccc caggttacgg cgtttacgct
     6061 cactacctgc ccgcgctgca gcggcggaat cgtggaatgg cgggttacac aaggtcgcgt
     6121 cgtattgctc gtttttatgg atgatgccgt taaaaattgc cccactctct ttttgccgac
     6181 gcagacgaat ggcgcggtta agccccggat tagcactgat aatcgcctgc gcactgctta
     6241 acgcctggct gctggtttca ctaccggtaa aacgccagcc atattcatgt acgccaatca
     6301 gcggataaat acagttcgca ccaacgccga tatccagaat gctggcattc gctggaattg
     6361 ttccgcttgc ttcagccagt aaatcggcaa ggtgatgaat ataatccgcc cggcccggta
     6421 ctggtgggca gagaaaaccg tcggggatat cccagttcgc tacggcgtaa aaatgggcca
     6481 gcaacgcctt attgagcgcc ttcaccgcca gcggattggc aaagtctacg ctttgctccc
     6541 cggcgggtgt aagcgtgagg aattgcctga gttcaggatt gacctgacaa agcgtggcga
     6601 gatcgtagcg gctgtgatga cggttgcgcg gatgcaaccc cggtttctgg gcggacatag
     6661 cattctcctt taagcagcgg cgtaagatac ccgcataacg cgacagggta aatatctggc
     6721 tgcgtttatc attccccaaa accttaacat ggctatggtt tatggcaatt catcataaaa
     6781 atcagcatta tgcatgtcat taatttgtct tattttgtaa aaatatatcg ttcaaaaaat
     6841 aagcacatct ctgtcaattt tttgcgtcat ttgcatcgcg caggcgacct acacttatca
     6901 ggcactaccc gttgtattta tgaggatgtc attatgaaaa agtgcctcac actactgatt
     6961 gccaccgtcc tgagcggaat ctctctcacg gcttatgccg cgcaaccgat gagtaacctg
     7021 gacagcggtc agctacggcc cgccggtacc gtttcggcaa ctggcgcatc aaacctaagc
     7081 gatctggagg ataaactggc agaaaaagcg cgcgaacaag gcgcgaaagg ttatgtcatt
     7141 aattccgcag gcggaaatga ccagatgttc ggtactgcaa ccatctacaa ataaccccac
     7201 caacgtaact gcattcgcct ctggatgcag ttttcgcctg ttttgtaatc aaaaaacatc
     7261 aaatatgaac tcaatgtaaa taaatgtatt tctttttcgc gcaatgggtg atagaaaatc
     7321 gcgccaagtg ataatgctta tcaaaattat tatcacttgc acgaacacta tcacgggatt
     7381 aacagtggca tcgcatccgc agagatgctt tctcgtggca gtgaaaattt caacatataa
     7441 gaaaaagtca cctgcaaaat ggaaaacaat cgcaatttcc ctgccagaca atttcattcg
     7501 ctcacgttct ttgccggtct ttgtattggc atcacgcctg tggctcaggc actcgccgcc
     7561 gaagggcaaa ctaacgcgga tgacacgctg gttgtcgaag catcaacgcc ttcgctttat
     7621 gcgccacaac aatctgccga tccgaaattc tcgcgtccgg tagcggatac tacccgcacg
     7681 atgacggtga tttctgaaca agtgattaaa gatcagggcg caaccaacct taccgacgcg
     7741 ctcaaaaacg cccccggcg
//
LOCUS       DAZPEP010000181         7718 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZPEP010000181 DAZPEP010000000
VERSION     DAZPEP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.182"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..643
                     /locus_tag="ACHUT9_003769"
     CDS             <1..643
                     /locus_tag="ACHUT9_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1350148.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGYSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUT9_003770"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="ACHUT9_003770"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFR1350149.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="ACHUT9_003771"
     CDS             2474..2986
                     /locus_tag="ACHUT9_003771"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFR1350150.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUT9_003772"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="ACHUT9_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFR1350151.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="ACHUT9_003773"
     CDS             5579..5782
                     /locus_tag="ACHUT9_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFR1350152.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUT9_003774"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="ACHUT9_003774"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFR1350153.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7718)
                     /locus_tag="ACHUT9_003775"
     CDS             complement(6827..>7718)
                     /locus_tag="ACHUT9_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HFR1350154.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKESQEVLHVQR"
BASE COUNT         1783 a         2104 c         2021 g         1810 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt tcgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggtta
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaacaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccgcactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcgatct
//
LOCUS       DAZPEP010000182         7708 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZPEP010000182 DAZPEP010000000
VERSION     DAZPEP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7708
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.183"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..304)
                     /locus_tag="ACHUT9_003776"
     CDS             complement(<1..304)
                     /locus_tag="ACHUT9_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1350155.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
     gene            complement(1125..1337)
                     /locus_tag="ACHUT9_003777"
     CDS             complement(1125..1337)
                     /locus_tag="ACHUT9_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HFR1350156.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(1413..2030)
                     /locus_tag="ACHUT9_003778"
     CDS             complement(1413..2030)
                     /locus_tag="ACHUT9_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFR1350157.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGAGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            2297..3796
                     /gene="ansP"
                     /locus_tag="ACHUT9_003779"
     CDS             2297..3796
                     /gene="ansP"
                     /locus_tag="ACHUT9_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFR1350158.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGMPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(3911..4972)
                     /locus_tag="ACHUT9_003780"
     CDS             complement(3911..4972)
                     /locus_tag="ACHUT9_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFR1350159.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFYTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            5214..7316
                     /gene="pqqU"
                     /locus_tag="ACHUT9_003781"
     CDS             5214..7316
                     /gene="pqqU"
                     /locus_tag="ACHUT9_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFR1350160.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDIP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAARRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="ACHUT9_003782"
     CDS             complement(7352..>7708)
                     /gene="mcbR"
                     /locus_tag="ACHUT9_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1350161.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
BASE COUNT         1964 a         1800 c         1856 g         2088 t
ORIGIN      
        1 tgcgccacag cggtgggcat accttcatgc tgtacagcat ctggtttttg tcgttctccc
       61 ccagttcaaa ccaggtcacc acacctttca cccggcgctg tacgtcatcg ccctgccata
      121 ccgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga aactgctggc
      181 tgaccagaga gagatcgagg gaaaagagcg aagagagtga ctggttcaaa tgaaaggaaa
      241 ccaccgcaaa agcatccggt ggcaggccgt ccacttccag tgtgaaacgt aatccggttg
      301 acatagcaac ctccgtgaaa atgaataata atccgcacat attgcatgta caacatgtcg
      361 ggcggatgag ataagcgagt cgacatctgg cgttaccggg gaatatcaga aaaatgtcat
      421 ggttatgtga tgatttagtc tgggatttta cttcagagaa acgggtgtct gtttagtcaa
      481 caaaataatc acaaacgcaa cggatgggtt ctcgacgtta ttcacgacag gctcgtacat
      541 tacagcgagt aataaaaaac ctgatatgca aagtcaaacc aatattcata atgtcttttt
      601 attcacaaca ataaataatt caataacaat cattgaaatg aatgatggtt tgtataagga
      661 atatacgcta ccgcccaccc tgataacatg atctgcacat tgccatctta acgtcgttaa
      721 acactggctt aatagtggat atttcaaatt aggaatttat taaattcacc tgatgaatat
      781 aataagcaaa tagcatagaa aataatggca actccccccg agaggaccat ttccaaagca
      841 ctgcaccacc acctggtgcc cggatgatct tgcttcagtg gtaagagtac ctgattcatt
      901 gttcccaaaa tgatgttttc cccggccccg ccaataatgt gcagctctca cgtgataatc
      961 tattgattag gttatattaa gaatcaatat ttaaaataca tccattttct tgaataaatc
     1021 aacatattta tttatcaatt gctgttttag ctctgactca tcaagctctc catatttgtc
     1081 ctatagataa ttgcgaataa atacagagaa cgccatttat ccttttagca tacctgccgg
     1141 atatttttat ttgcattaat attaattgca tctccaactc cgagcatatc catcattaaa
     1201 aactttttga gattatagaa tacctgtgat aacaagtgaa acatctgcac aatgtgaata
     1261 gttagtttct tttcagagga aatgaaatag acaagctgac tgaaggggag agtgatataa
     1321 atcaaaaaac ataacatgaa tttaattaat tacgcccatc attgtggata aagttactga
     1381 ataataaata acataatcct ctggtatccc actcaaataa tttcattcct ttttaatact
     1441 tcttgtaatt tcggtaactg acatacagca tcagcaatcg cactgatatt cgtggcatta
     1501 tcctgaaacc attcatgccc agccccccat gtgcgcatag tgcaaagata acagtccacc
     1561 agcgttagtt gctcaccaaa cgcataaggt tcagcggtca attgtgaatt cagccagata
     1621 taaagcgatt tacgatattc aataacattc ttctttaact gttcaggtgc gtcaggagcc
     1681 caacgctcag ggtaatcggc gaaagtgaat gttggataga cattagcaac cagccagacc
     1741 aatagccgtt gaaactgctg acgttcggcg cgcccaaccg gtggcgcaag gtccgggcga
     1801 cgatcgagaa ccatcaatgc aatcgccgct gtctccgtca taatctcgtc attttccagc
     1861 gccagtgtcg gaatctgaca caacgggttc agggttttca ataactcgcg tgaagttccc
     1921 tcgtggtcaa aaccactgac atcaacgaac tgataaggaa tatcagccag tgttagcatc
     1981 agctcactga ttgtagagcc ccatccgggt acgccataca ccttaatcat atgcccctca
     2041 caatctttta aagattaagt gtaggacagt acacaggtta accagcaaaa gtatttgtca
     2101 ttttttcgtc agttgtatga atccccttca ggattgtaag gggatgtttt gcatcgtata
     2161 tcgataacgg gtaggatgct gcgccgatag ttttataccc ataaagaata atggtgataa
     2221 ctatcatcgc caggatgaat aaacattgtt catggcaact tatatgactt tttcattaaa
     2281 gcaatcaggg agagcaatga gtaaacacga caccgacact tcagatcaac acgccgcgaa
     2341 acgccgctgg cttaatgccc acgaagaggg gtatcacaaa gcgatgggca atcgccaggt
     2401 acagatgatc gccattggcg gcgcgattgg caccggcttg tttttaggtg caggagcccg
     2461 actgcaaatg gcgggtccag cactggcact ggtttattta atttgtggct tgttttcgtt
     2521 ttttattctg cgtgcattgg gtgagctggt gctacaccgc ccttccagtg gcagttttgt
     2581 ctcttatgcc cgtgagtttt tgggtgagaa agccgcttat gttgctggct ggatgtactt
     2641 catcaactgg gcgatgacgg ggattgttga tattaccgcc gtcgctctgt atatgcatta
     2701 ctggggtgcg tttggcggcg tgccgcagtg ggtctttgcg ctcgctgcac ttaccatcgt
     2761 tggcaccatg aatatgatcg gtgtgaaatg gtttgcggag atggagttct ggtttgcgct
     2821 tattaaagtg ctcgccattg tgaccttttt ggtcgtgggt acagtgttcc tcggtagtgg
     2881 tcagccgctg gatggcaaca ccactggctt tcatttaatc accgataatg gcggcttctt
     2941 cccccacggt ttgctgcctg cgctggtgtt gattcagggc gtagtgtttg cttttgcctc
     3001 cattgaaatg gtgggtacag ctgccggaga atgtaaagat ccgcagacca tggtgcctaa
     3061 agccattaac agtgtgattt ggcgtattgg cctgttttac gtcggctccg tggtgttgct
     3121 ggttatgtta ttgccgtgga gcgcgtatca ggcggggcaa agtccgttcg tgacgttttt
     3181 ctctaaactg ggtatgccat atatcggcag cattatgaac attgtggtgc tgaccgctgc
     3241 cctctccagc ctgaactcag gtctgtactg caccgggcgt attctgcgct cgatggcgat
     3301 gggcggttcc gcaccgagtt ttatggcgaa aatgagtcgt cagcatgtgc cgtatgccgg
     3361 gattctggcg acactggttg tgtatgtcgt cggcgtattc ctcaactatc tggtgccgtc
     3421 gcgcgtattt gagattgtgt tgaacttcgc gtcgctggga atcatcgctt catgggcgtt
     3481 tatcatcgtg tgccagatgc gcctgcgtaa agcgattaaa gaaggcaaag cagcggatgt
     3541 cagttttaaa ctgcctggcg cgcccttcac ttcctggctg acattactgt ttttactgag
     3601 tgtccttgtg ctgatggcgt tcgattaccc gaacgggact tatactatcg cggcgctgcc
     3661 gattatcggt attctgctgg ttataggctg gtttggtgtg cgcaaacgcg ttgctgaaat
     3721 tcacagcact gcgccagtcg tcgaggaaga tgaagaaaaa caggaaattg tgtttaagcc
     3781 tgaaacggcg agctaattgc aaaattgccc ggctacgtgc cgggcaaatt tagattactg
     3841 acgaaggtgt gccgcatgcg gcgtgataaa agtcaggcaa gtctctccgt tttcaccctc
     3901 gccatatcta ttacagcgca atacgaatca catcgtctgg ctgggtagct tcctgctgtt
     3961 tagtggattt ttgtttcaca ctgacataca gcgttttgcc atcggcagac agcgccaggc
     4021 tgtttggatg agtcggcgta tagaacgttt tcactacttt atagcttttc gcgtcaatca
     4081 cactgacttt acctgcctga cgatgcgtta cgtaggcttc attacgcgct gggttaaaca
     4141 gcacagccag tgattccggt gctgcaacct tcgccagaat attgccatta cgggtatcga
     4201 ccactaacac ttctgcggct ttagaatcgg tgataaatgc acgctgcctg gcggtatcaa
     4261 ggctaatgtt gataaagaag tgctctttgc cgtcatccag cagcttttta cggctgagga
     4321 ttttattgtc ggcggtgtcg atggtaatca attcgccgtc agcgttagtg gtgtaaagac
     4381 gtttgccttt gctatcaagc gccagaccgg tactcatttt accggtgttc tggatggcgg
     4441 ttttcagttt gatattctcg ccatcaacga cccaaatcac gctctcttta ccaataccac
     4501 tgatgtaaac ggtgttcgtg gcatcgtcag ctaccagctc acgcggttgc agcgggcgca
     4561 cctcttccgt acgcttacga tcatccagca ccagacggcc tttcacctcg cccgttttgg
     4621 catctatcgc cgtgaccgcg ctgtttacag tgttaccaaa ccacagtgtc tgagtcgtgt
     4681 tattgatggt gacaccaaac ggcttgagat cgttatggat cgcctgcgtc acttccagag
     4741 tgaccggatc aagacgataa accacgccgc ctttatccag tttgcggctt tgcgaagtgg
     4801 cgagccacag cgcgttttct tgctggctat aagccatttc gtaggcacct ttacctaccg
     4861 ctttacgcag catttcttct gcggcctgcg tactgaatga tgaagcaaca agcaatgaac
     4921 ctaacagtaa tgaaccacgc aggcgcgatg aaaacagatg acgtaaatgc atgacgactc
     4981 cctttgatga accgatattt ttgctgcttc acaatgcttc gctaagcgta atcatcgtta
     5041 cgctcttgtt ataaatggga atggtaatca ttattttctc aaatgtggag catttcttgc
     5101 cgttacgact acaattttct caattactta acgtatcgtg ccgtttcgtt ttccatgaat
     5161 ttacaatttt attaccataa cggcgttttc ccattcttaa cctggctttt ttcatgaaga
     5221 ttttttccgt ccgacagacc gttttgcccg cactacttgc cctttccccc gttgtttttg
     5281 ccgctgatga acagaccatg attgtcagtg ccgcaccgca ggtggtttca gaactggata
     5341 tcccagcagc agtaagcgtg gtggatggcg aggagatgcg cctggcaaca ccgcgcatta
     5401 acttgtccga atcactgacc gacgtgcctg gtttgcaggt acaaaaccgg cagaactatg
     5461 cgcaagattt acagctgtcg attcgcggat ttggctcccg ctccacttac ggtattcgcg
     5521 gtattcgcct gtatgtggac ggtattcccg ccaccatgcc cgacgggcaa gggcaaacat
     5581 ccaacatcga tttaagcagt gtgcaaaatg tggaagtgct gcgtggcccc ttctctgccc
     5641 tgtatggcaa cgcgtctggt ggggtaatga atgtcaccac ccagaccgga caacagccac
     5701 caaccattga agccagtagt tactacggca gttttggcag ctggcgctat gggctgaaag
     5761 caacgggcgc aacgggagac ggcacacagc ctggcgatgt cgattacacc gtctcaacca
     5821 cgcgttttac gacccacggc tatcgtgacc atagtggcgc acagaaaaat ttagccaatg
     5881 ccaaactggg cgtacgcatt gatgaagcca gcaaattaag tctgattttc aatagtgtgg
     5941 atatcaaagc agatgacccg ggtgggctaa ccaaagcaga atggaaggct aatccacaac
     6001 aagcgcctcg tgcagaacag tacgacacgc gaaaaaccat caagcaaact caggctgggt
     6061 tgcgctatga gcgtagcctg agcgcgcaag atgatatgag tgtgatgatg tatgccggag
     6121 agcgagaaac gacccagtac cagtcaatac ccatggcacc acaacttaac ccgtcacatg
     6181 cgggcggcgt gattaccctg caacgccatt accaggggat agacagccgc tggacacacc
     6241 gtggtgaact gggcgttccg gtcacgttca ctaccggcct gaactacgaa aacatgagtg
     6301 aaaaccgcaa gggctacaat aacttccgcc tgaatagcgg catgccggag tacgggcaaa
     6361 aaggtgagtt gcgtcgcgac gaacgcaatc tgatgtggaa catcgatccc tatttacaga
     6421 cgcagtggca gctgagcgaa aaactgtcgc tggatgctgg cgtgcgctac agctccgtgt
     6481 ggtttgattc caacgaccat tacgttactc cgggtaacgg cgatgacagc ggtgatgcca
     6541 gttatcataa atggctacct gccggttcgt taaaatatgc aatgaccgat gcctggaata
     6601 tctatctggc agccaggcga ggttttgaaa cgccgacgat taatgagctg tcttatcgtg
     6661 ctgatgggca aagcggtatg aactttggtt taaaaccatc caccaacgat acaattgaga
     6721 tcggcagtaa aacgcgtatt ggtgatgggc tgcttagtct cgcattgttt cagaccgaca
     6781 ctgatgatga aattgttgtc gatagcagta gcggtgggcg tacgacttac aaaaatgccg
     6841 gaaagacccg tcgtcaaggc gctgaactgg catgggatca acgtttcgca ggagattttc
     6901 gcgtaaacgc gtcctggacc tggcttgatg cgacctatcg cagcaatgtt tgcaatgaac
     6961 aggattgtaa cggtaatcgg atgccaggga tcgcccgtaa tatgggcttt gcgtcgatag
     7021 gttatgtacc ggaagatggt tggtatgcag gcacggaagc gcgttatatg ggcgatatta
     7081 tggcagatga tgaaaatacg gcaaaagcgc cgtcttatac tctcgtcggc ttattcaccg
     7141 ggtataaata caattaccac aatttaactg tggatttatt tggtcgtgtc gataatttat
     7201 tcgataaaga atacgttggt tctgtcattg tcaatgagtc aaacgggcga tattacgaac
     7261 cttcgcccgg acgaaattat ggtgtcggca tgaatattgc gtggagattt gagtaattac
     7321 cttgatgccc ggtatttgcc gggcatttac tttaacgatt gtattgctgg tataaaatag
     7381 caatattttg ttgcataatt tcagcaagac aatgtctgca tccctctttg tcttttgctt
     7441 ttaacgcagc gagtaacaga tgatagtttt ctatatgctc ccgtaattcc gctggattaa
     7501 tcgcttcata gagataatgt aaaccaggcc ccatcctgac ccacagttgc tcaatcatct
     7561 cacacaggat gggcatattt gagcgatgat aaatcgctaa gcgaaatagc ctgtttacat
     7621 taatgatttg ttccatgtcg cccttttctt gcgcctgctg taatttctcg agcaattcct
     7681 ggagttccgt caggtcttgc ggggtgag
//
LOCUS       DAZPEP010000183         7704 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZPEP010000183 DAZPEP010000000
VERSION     DAZPEP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.184"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(198..749)
                     /locus_tag="ACHUT9_003783"
     CDS             complement(198..749)
                     /locus_tag="ACHUT9_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1350162.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
     gene            1336..1683
                     /locus_tag="ACHUT9_003784"
                     /pseudo
     CDS             1336..1683
                     /locus_tag="ACHUT9_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
     gene            1736..2131
                     /locus_tag="ACHUT9_003785"
     CDS             1736..2131
                     /locus_tag="ACHUT9_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFR1350163.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            2172..2915
                     /gene="cmoA"
                     /locus_tag="ACHUT9_003786"
     CDS             2172..2915
                     /gene="cmoA"
                     /locus_tag="ACHUT9_003786"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFR1350164.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2912..3883
                     /gene="cmoB"
                     /locus_tag="ACHUT9_003787"
     CDS             2912..3883
                     /gene="cmoB"
                     /locus_tag="ACHUT9_003787"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFR1350165.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4048..6477)
                     /gene="torZ"
                     /locus_tag="ACHUT9_003788"
     CDS             complement(4048..6477)
                     /gene="torZ"
                     /locus_tag="ACHUT9_003788"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFR1350166.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKDGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTTRIQHPMVRKSYLDNPLQPT
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIDFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLRGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6502..7602)
                     /locus_tag="ACHUT9_003789"
     CDS             complement(6502..7602)
                     /locus_tag="ACHUT9_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFR1350167.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         1833 a         1970 c         1751 g         2150 t
ORIGIN      
        1 gagtctgaaa atttttataa aaaatgacta gccctctttg aatctcatct tccccgaatc
       61 ttcgaaaata tctgtctagt tgattcggta gaatctacct catgatgtat acgtgtttag
      121 tacccgtcac accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca
      181 tgtaactatt cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctagc
      241 tcttcctgaa tctgcaacct cgttttacct tgtgaaataa gcatctgtac agcctctgaa
      301 gaagctttta caggtcttcc catatgtacc ccttcagctt tagcacgttc aataccagcc
      361 tttctacgct ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac
      421 attgcttcac ccatagcgct tgataagtca aatccttctc taaggctgat aactgtcaca
      481 cctttagcct gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg tccaagacgg
      541 tctacagcta ccacaactac ggtgtcacct tctcgtgcat aagctaacaa actgctgaaa
      601 ccgtttctta aagaagcttt gacagaacca ctgacagctt catcagcgaa ccatttagca
      661 atgttgtacc ctgcctcttc aatttgcatt tgctggttag tgatggattg gtcatctgta
      721 ctcaccctgc aatatccaag tatcgccata atgttcccct tagaagtctc aaaagttagt
      781 ctcataagtt tagctgtctc ataatccaaa aacaaccttt tgagactact tgagggtgca
      841 ttctaccgtt acgttgaaag tgtctcaaaa ggacttcctt atgagactgg ttaatagagc
      901 gctatgaaga ttcaggaggg taactataac catcactttc taactttcac gtaggaaatc
      961 gttaagtttc ttgatagtta caccatgtga ccaactatct atatatcgtt atatagtcct
     1021 gtatatagca ctatatgtag tgtctggaga gggacgacta acacgataca gtttaccctg
     1081 aaaagagact tcacagattc tttatatatt tcaataagtt atgctaaaaa gagactatac
     1141 cgatactgca tagaatttgt gtaggattgc atagatactt ggaaaaacac cgttaaattg
     1201 catagtcatt cacctgatat tgtcctgttt ttacaggtag ttgattcgga tgttctagga
     1261 atcatcctca tagttgattc ggttagttgg gcttgtacca acccctaatc aacctcatag
     1321 tgtatttaga ccttgatgat tttagacagc cgccctgttc atgcagcacg tccacacagt
     1381 gaagctattc gcgacgctca acgaaaaaaa cctaaagttc cggtacatgc tgtactgacg
     1441 gcgacaaatc cgctgattcg ttttatcggt agtgatgata tgacgcaaaa ccgggaatta
     1501 tttcaggtct ggttacagaa attagcgcag tggcatcaga ccactacgcc ttatcttttt
     1561 ttacatacgc cagacattgc ccaggccccg gaactggtac ataccctgtg ggaagactta
     1621 cgtaaaacgc ttccagagat cggagcagtt ccggctattc cacagcaatc ttctcttttc
     1681 tgaatttgcc acctatcata gacaggtgcc atcggccatt ttaaagggag tttgtatggt
     1741 aagcgcgcta tatgccgttt taagtgcgtt gttattaatg aagttctctt ttgatgtcgt
     1801 tcgcctgcga atgcagtacc gcgttgccta tggtgacggt ggttttagcg aactgcaaag
     1861 cgctattcgc attcatggta acgcggtgga atatattccc atcgcgattg tgctgatgct
     1921 gtttatggaa atgaatggcg cagaaacctg gatggtgcat atttgcggca tcgttttgct
     1981 tgctggtcgt ctgatgcatt attacggttt tcatcaccgt ctgtttcgct ggcgacgttc
     2041 cggcatgagc gccacctggt gtgcgctgtt gctgatggtg ctggcgaatc tttggtatat
     2101 gccctgggag ttggttttct ccctgcgtta gcgcacaata cgccactttc tttttcccgg
     2161 atttttacgt tatgtctcac cgcgacacgc tattttctgc ccctatcgcc agactgggcg
     2221 actggacctt tgatgaacgg gtagctgaag tcttcccgga tatgatccag cgttccgttc
     2281 ccggctattc caatattatt tccatgattg gtatgttagc cgagcgcttc gttcaacctg
     2341 gtacgcaggt ttacgatctg ggttgttctc tgggcgcggc gacgctctcg gtgcgtcgca
     2401 acattcatca tgataattgc aaaattattg ccatcgacaa ctccccggcg atgattgaac
     2461 gctgcagtcg tcatattgac gcctataaag cccctacgcc agtagacgtc attgaaggtg
     2521 atattcgcga tatcgccatt gaaaacgcat cgatggtggt gctgaatttt accctgcaat
     2581 tcctggaacc ttccgagcgc caggcattac tggataaaat ttatcaagga ctgaacccgg
     2641 gcggcgcgct ggtgctttcg gaaaaattca gtttcgaaga tgccaaagtt ggtgaactgt
     2701 tgttcaacat gcaccacgac tttaaacgtg ccaacggtta cagcgaactg gagatcagcc
     2761 agaagcgcag catgctggaa aacgtgatgc tgaccgattc cgtggaaacc cataaagcac
     2821 gcctgcataa agccggtttt gagcatagcg agctgtggtt ccagtgcttt aactttggtt
     2881 cactggtggc attaaaagca gaggacgctg catgatcgac tttggtaact tttattctct
     2941 gattgccaaa aatcatcttt cacactggct cgaaacgctg cccgcgcaga ttgctaactg
     3001 gcagcgcgag cagcagcacg ggctgtttaa gcagtggtcc aacgcggtgg aatttctgcc
     3061 tgaaattaaa ccgtatcgtc tggatttatt gcatagcgta accgccgaaa gcgaagagcc
     3121 actgagcacc gggcaaatta aacgcattga aacgcttatg cggaacctga tgccatggcg
     3181 caaagggccg ttctcactgt atggcgtcaa catcgatacc gaatggcgtt ccgactggaa
     3241 atgggatcgt gttctgcccc atctttctga tttaaccgga cgcaccattc tggatgtcgg
     3301 ctgcggcagc ggttatcaca tgtggcgcat gattggcgca ggcgcgcacc tcgcggtggg
     3361 gatcgaccct acgcaactgt ttctgtgcca gtttgaagcg gtgcgtaaac tactgggtaa
     3421 cgatcagcgc gcacatttgt taccgttagg tattgaacag cttccggcac tgaaagcctt
     3481 tgataccgtc ttttcgatgg gcgtgcttta ccatcgccgt tcgccgctgg agcatctctg
     3541 gcagttgaaa gatcaactgg tgaatgaggg tgaactggtg ctggaaacgc tggttattga
     3601 tggcgatgag aacacggtgc tggtaccagg cgatcgttac gcgcaaatgc gtaatgtcta
     3661 tttcatccct tccgcgctgg cgctgaaaaa ctggctgaag aagtgtggtt ttgtagatat
     3721 ccgcattgtg gatgtgtgcg ttaccactac tgaagagcag cgacgcactg aatggatggt
     3781 taccgaatct ctgtccgatt tcctcgaccc acatgatccg agtaagacag ttgaaggtta
     3841 tcctgcgcct aaacgcgcgg tgctgattgc gcgcaagccg taaaggtctg gtattactgc
     3901 cggatgcggc gtgaatgcct tatccggcct acaaaccctt gctaattcaa tctattgcag
     3961 ggactatgta ggcctgataa gcatagcgca tcaggcagtt ttacgtttgc ataacctcag
     4021 cgcccgtttc cgggcgctat tcacttctta cgcctgaaca gcaggcttat caaatgccgt
     4081 taacttcggt gcgttacccg tatatttttc gatatacacc agcgcagagt taccggcaca
     4141 ggcatttgcc agctgcgagc tggggatatc cgccgttaac acgttcgcac tgccgttttt
     4201 acacaagccg ttttccagat ctggccatgc accttcatgc acgcatacca cgcctttttt
     4261 gatcccgtca gtcaccaccg cgcctgtcaa aatctgacca cgtttgttcc agacacgcac
     4321 cagatcgcca ttcgcaatac caaatcgagc agcatcttcg gtgtgaatag tgatcggttc
     4381 acgatcggca accgcatatt ttttacgcag ttccgcatag ttaagctgac tgtgtaaacg
     4441 gtgtgccgga tgtgcggtca gaagctgcaa ctgcttctcg tcggcggtac ccttccactc
     4501 atcaggcgca agccaggttg ggtgtgccgg gcaatcctta tagccaaatt tttccagcgt
     4561 tttggagtaa atctcaattt tgccacttgg cgtacccagc gcatttttca ccggatcggc
     4621 gcggaaatca ccataacgaa cgtactgttc gttcttctcg ctgcggcgca tttcgatcag
     4681 tttattttgc tgccagaagg cattaaacat tggcatagtg acgcgttgcg cacgggcccc
     4741 tttctgagcg gcatcataga aaaatttcag ccacgccatt tcatctttac cttcggtata
     4801 gatctctttt ccgccaggtt tgagtaattc cgcaagatcg gcaaacacat caaaatcgtt
     4861 tcgcgcttca aattgcggag caacagcctg cttcatcggc acaatatgct ggttgctgta
     4921 atcaccggtc atcgtcaagt cattgcgctc aaacgatgtg gtgatcggta atacgatatc
     4981 tgcgtgttta gcagctgcgg tccagtagca ttcagaaacg acgatcatct ccggtttctg
     5041 ccatgcttta atcagacgat tagtatcctg gtgatgggta aagttaccgc cacccgccca
     5101 ccagatcatt ttgatattcg gatacgtttg ctctttaccg ttatgctgat atttgccgcc
     5161 aggattttcc agcgcatcga cgatacgggc aacaggaatc gccctcattc cgccatcatc
     5221 agcggcctcg ctggcctgtc cggcaatcgc cgcagacatt tccggcagca cgccgccaac
     5281 acgtgtcggg ttgccaccat tggagtaatg ataagagaaa ccaaaaccgc cgcccggtgt
     5341 accaatctgc cctaacatcg ccgccagcgt caccagcatc cagtgtttct gttcaccata
     5401 ttgctggcgc tgaattcccc agcctgccat cagcattgtg cgattcgccg ccatcagttc
     5461 agcgagtttg actatctgcg cttccggtac gccagtaatt tccgccgccc aggccgcgct
     5521 cttcggcgta ttatcgctct tacctctcag atactcttca aactgcggat acccggtagt
     5581 gtatttttca aggaacactt tatcgtgttt gccttgtgtc atcagggtat gcgcaatccc
     5641 taacatcagt gccacgtcgg tgcccatatt cggcgcgatc caggtggcat tatcgccaaa
     5701 gaagtcgatg gtttcggagc ggataggatc aatggcaatc actggtttgc cagatttttt
     5761 cagctgatgg aagtattcca gcccttgctc atcggtactg ctccaggcaa tttttaaggt
     5821 attcagcggg ttcatccccc acagcaccac aacctggctg ttttccagaa tcagcggcca
     5881 ggaggtctgc tgttcataca cctctacaga accgaccaca tgcggcatga tcacctgtgc
     5941 cgccccggtt gaataatcgc cgctatgccc ggaataaccg cccgccaggt tcatataacg
     6001 ttgcagtaag gtttgcgctt tatgcaacac gccagaagag cgccagccgt atgatccggc
     6061 aaaaatagcc gatggtccgt tagctttgcg aatgcgatcg tgttgctcat gaatcagttt
     6121 taatgcttgt tcccagctca cctgtacata ggtatcttcg ccacgacctt tcgtcggttg
     6181 cagtggatta tcgagatagc tttttctcac catcggatgc tgaatacgcg tcgtggtgtg
     6241 tacctgatcc gccgccgtag actgtaagga attcggtatg gttttcgcca gcgcgcctgt
     6301 tgaagaaaca atcttgccgt ccttcacttc tacgttcatc gctccccaac gtcccgcggt
     6361 gaggatttta ccgtccttct cttctgccca tgcggggagc ggtgctgccg atgtcaccac
     6421 cagcgctcca gcggcaatac cgctgtgttt aataaattca cgtcttgtta atgtcataac
     6481 ttcctccctg atcaacgagg atcactgttt ctcggtaata tctttggcgt tgtactggaa
     6541 ataccgcgtt aaaatgtcca gttcgttttc gctcatgctg gttcgtgccc ccattccttt
     6601 ggcaatggac ggccacgcat tgacggtgta atggtcggcg gcaatagggg catgacaacc
     6661 agcgcaatag gtatcgtcaa gtttttcagc gtattgccat agcggtttac ggtccgctaa
     6721 tccgggatcg gtaagcgcac cctgtaaaga cgcctgacgc cattgattgc cgtattcgtc
     6781 agcctgccat tccccgttta ccgtgagcgc cttgatacct tcttcactta atgtggctag
     6841 cgccagccgt tgacctgccg ccaggtagag cgtgttttca ctgccctgca tttgataacc
     6901 ctgcaacaga acgatcggct gtttgccact ggcatcaacg acggtgagat cggtaccagg
     6961 attcacggta gccagctcgc ctatgcgaga agttttgaaa ggataaatat gtgcgccatt
     7021 agtcactgaa gtagcggcct gactttccag ctcatgcgcc gcgttgtcat ccatttttat
     7081 ttctggcgga aaatgggcaa tgcctttatg acaatcgata caggtttcgc cgcccttttg
     7141 tgctttgtta tgcattttct gcgcagattc actttgcgag gcaatatcca tggcatcaaa
     7201 agaatggcaa ctacggcacg ttgcagagtc agttgctttt aattctttcc atactgtttc
     7261 ggccatttcc tggcgatgag tttcgaactt atcgtcactg tctattttgc cgctaacaaa
     7321 ttcatgataa atatctttag atgcttttaa tttagcaaat aaataatcca tccctgactt
     7381 tggaatatgg caatctgcac attccgcacg tatccctttc tggttcgaaa agtggacagt
     7441 tccctgatat tcctcaaaag gtttactcat cgagtggcag gaaaggcaaa atgctgtatc
     7501 cgacgtttta tgtaaggctt tttgcgccag caataacccg ccgccaccaa ccacaaccgc
     7561 tatcagcaaa aacaataacc caatgcgttt tttccctcgc atagattcct ctaaataaat
     7621 tccagaacac cgaaatcatt gtataattaa atacacaacg aattaaccgc ttgcataact
     7681 cggcacaaca ctgcctgaaa taat
//
LOCUS       DAZPEP010000184         7647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZPEP010000184 DAZPEP010000000
VERSION     DAZPEP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.185"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(188..934)
                     /gene="cutC"
                     /locus_tag="ACHUT9_003790"
     CDS             complement(188..934)
                     /gene="cutC"
                     /locus_tag="ACHUT9_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFR1350168.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(948..1514)
                     /locus_tag="ACHUT9_003791"
     CDS             complement(948..1514)
                     /locus_tag="ACHUT9_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFR1350169.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1730..3466
                     /gene="argS"
                     /locus_tag="ACHUT9_003792"
     CDS             1730..3466
                     /gene="argS"
                     /locus_tag="ACHUT9_003792"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFR1350170.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            complement(3508..3552)
                     /gene="yecV"
                     /locus_tag="ACHUT9_003793"
     CDS             complement(3508..3552)
                     /gene="yecV"
                     /locus_tag="ACHUT9_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFR1350171.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3643..4131
                     /locus_tag="ACHUT9_003794"
     CDS             3643..4131
                     /locus_tag="ACHUT9_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFR1350172.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIENDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4251..4643)
                     /gene="flhe"
                     /locus_tag="ACHUT9_003795"
     CDS             complement(4251..4643)
                     /gene="flhe"
                     /locus_tag="ACHUT9_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFR1350173.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4643..6721)
                     /gene="flhA"
                     /locus_tag="ACHUT9_003796"
     CDS             complement(4643..6721)
                     /gene="flhA"
                     /locus_tag="ACHUT9_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFR1350174.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6714..>7647)
                     /gene="flhB"
                     /locus_tag="ACHUT9_003797"
     CDS             complement(6714..>7647)
                     /gene="flhB"
                     /locus_tag="ACHUT9_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFR1350175.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1947 a         2073 c         1869 g         1758 t
ORIGIN      
        1 tcccataatc aatgtgaata taaaaaatat tattagcaca acgctatgtt cactgtcacc
       61 cgctcaacag atcacggtta tcgttcgttt tttatactgc tcagggataa aaaaaggccc
      121 ctgttgaaat tgcaggggcc tggtacgagc aagcatcata ttgggcgaca tgatgcaacg
      181 gtaaaaatca tttggcctga tggcgttcaa tgattccttt catttcagca accgccgccc
      241 cgtctacgat ataacgcgaa tactcgtccg cgtgttcatc tgatgacatg gacaatcctt
      301 gattacgata acgcatcggt gaggcttgcc acgctcccgc ggagctatgg acttccagca
      361 ctccggcatc gaggaagtgg tgcaggtttt ctgcacggac tcctgctccg gccataatga
      421 ttggagcatc acgatgggca ataagttcca taatttttga taaaccttgc agtgcgtctg
      481 atttttgccc tgatgtcagt acccgggcaa tgcccaattc cgcaagatta ttgagtgtat
      541 ataaagggtt agcgcacata tcgaaggcgc gatgaaaagt cactgccagc ggaccggcag
      601 cagccattat tttttccatt cgtggcatat cgacattccc gtcaacatcg agaacgcccg
      661 tcaccagtcc aggaaaacct aattcgcgga ccgtgcgcac atcctcaaga atggcggcaa
      721 actcaccgtc gctgtagcaa aaatcaccac cgcgtgggcg aattatcgga tgcacaggga
      781 tcgtcacccg ctggcgcacg gatttcagta cccccaacga cggcgttaag cccccctctt
      841 ttggggctgc gcataattca actctgtctg cgccgttttg ctgcgccgtt agtgcacatt
      901 ccatgctgta acagcaaatt tccagtaatg ccatttttgc tccttaatta cgctgactgc
      961 tcgctggcaa cgatctcttc aatggaccac ggatgaaact taatagttgt tttgccatcg
     1021 gtgaccgcca gcgtcggatt cggtaatcgc tcatgctcac cttttggtga actggtttta
     1081 actgagatcc ccggtaggct gagtccttca tcagaaagca atgccagcgc acgggcattc
     1141 agcgtttctg gatcacccag cagaacgatt tcaatatgtt cccaaccttc gtgtgggtaa
     1201 cgtttttccc cgggccacgg tagctccaca atagaaaact gccagtgcgc aacctgtacc
     1261 ggttcatgca atttaaacag acaaatcggt ctgccattga tcatattttc tgacaaaagc
     1321 tcgccacact gttcaaaccc gcgacgccag cgttcagcag tggcgttttg atggcaacgc
     1381 aaagaaatgt gatcggcagt cagcggagtg atattcaacc ccagacggag ggaaagttca
     1441 tctaatgcgt gggtaaatcg cggtaaatcc gatgcaatat cctgcagctc gtcgatagat
     1501 tgccagttcg ccataatcac tcttcgtctt tcagtaaaag cgttaattta ccctgttgcc
     1561 ctgcgccaac caaccgctga tttcacgccg cttctgatgc aatagtgaaa acggcaatac
     1621 gccacgcgca cgttgctgac gaaaacagcc atttgcagta tactcccgcc ctaatttctt
     1681 taactggtgc gggcaatttt tgctcgcttc atcaatgtaa ggtattccgg tgaatattca
     1741 ggctcttctc tcagaaaaag tccgtcaggc catgattgcg gcaggcgcgc ctgcggattg
     1801 cgaaccgcag gttcgtcatt cagcaaaagt tcagttcggc gactatcagg ctaacggcat
     1861 gatggcagtt gctaaaaaac tgggtatgac accgcgacaa ttagcagagc aggtgctgac
     1921 tcatctggat cttaacggta tcgccagcaa agttgagatc gccggtccag gctttatcaa
     1981 cattttcctt gatccggcat tcctggctga acatgttcag caggcgctgg cgtccgatcg
     2041 tctcggtgtt tctacgccag aaaaacagac cattgtggtt gactactctg cgccaaacgt
     2101 ggcgaaagag atgcatgtcg gtcacctgcg ctctaccatt attggtgacg cagcagtgcg
     2161 tactctggag ttcctcggtc acaaagtgat tcgcgcaaac cacgtcggcg actggggcac
     2221 tcagttcggt atgctgattg catggctgga aaagcagcag caggaaaacg ccggtgatga
     2281 aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt acgacgaaga
     2341 tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg gtgacgaata
     2401 tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc agatcaccta
     2461 cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg ggcgaaagcc tctacaaccc
     2521 aatgctgcca ggaattgtgg cggatctcaa agccaaaggt ctggcagtag aaagcgaagg
     2581 ggcgaccgtc gtattccttg atgagtttaa aaacaaggaa ggcgaaccga tgggcgtgat
     2641 cattcagaag aaagatggcg gctatctcta caccaccact gatatcgcct gtgcgaaata
     2701 tcgttatgaa acactgcatg ccgatcgcgt gctgtattac atcgactccc gtcagcatca
     2761 acacctgatg caggcatggg cgatcgtccg taaagcaggc tatgtaccgg aatccgtacc
     2821 gctggaacac cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt tcaaaacccg
     2881 cgcgggtggt acagtgaaac tggccgatct gctggatgaa gccctggaac gtgcacgccg
     2941 tctggtggca gaaaagaacc cggatatgcc agctgacgag ctggaaaaac tggctaacgc
     3001 ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca cggactacat
     3061 cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca tgcagtatgc
     3121 ttacacgcgt gtattgtccg tgttccgtaa ggcagaaatt aacgaagagc aactggctgc
     3181 agctccggta atcatccgtg aagatcgtga agcgcaactg gcagctcgcc tgctgcagtt
     3241 tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtgatgt gtgcttacct
     3301 gtacgatctg gctggtctgt tctctggctt ctacgagcac tgcccgatcc tcagcgcaga
     3361 aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga agacgctaaa
     3421 gctgggtctg gatacgctgg gtattgagac agtagagcgt atgtaatcga tttttcgtga
     3481 gagtaaagcc tgatcagggt tacctgatca ggctttttta ttgccatcta aatgtattct
     3541 gaaaatggac atgccattgt tttctgactg ttggataaga ggccagaagc gtaatatccg
     3601 gccccaggga aacgataacg gttgaattta aggaataccg cagtgtttaa atttcttgta
     3661 ttaacattag gcattatctc ttgccaggct tacgcagaag atacggttat agaaaacgac
     3721 catgacattt cagccatcaa agattgttgg caaaaaaatt cagatgatga tactgacatt
     3781 aacgtgatca aatcatgcct gcgacaagaa tacaatctcg tcgatgcgca attaaataaa
     3841 gcctatggtg aagcttatcg ttatatagaa caagtgccac gcacaggtgt aaaaaaacct
     3901 gataccgaac aacttaactt gcttaaaaaa tcacagcgag cctggctgga ttttagggac
     3961 aaagaatgtg aattaatcct ttcaaatgag gacgttcagg atttaagtga cccttattct
     4021 gaatcagaat ggctctcatg tatgatcata cagaccaata cgcgaactcg ccagttgcag
     4081 ctataccgta actctgaaga tttttatcca agccctttga caagaggata attcacatct
     4141 ttttggcatg ttttgttgca agctattcct gataaataat tgcaacaaga catcgagcct
     4201 ttttcactga gttattaaac atactcgcga gcgcgtaatt tttttgtcct tcagcgataa
     4261 ttcacaatca cttcattacg ttgtaccttt agcggtggaa ttaaccgccc accgcctggc
     4321 acttcccaga taaatcgcaa cggttctgct gccgctatac cggaaaaggc cacggtggtt
     4381 ccgctctgcc cctctaattc gacacaacga gattgcgaac acaagcgaac ccgcagtcct
     4441 gaaggtgtcg ggccgataag ctgataacgc cacgctacca gcgtcatcaa tcctgaagca
     4501 ggttgtcgcg tagaaagagg cgcagacgac atcgactcac cgcgatgatt tagcgtaatt
     4561 cctacactac ttgcctgcca catcccctcc ccggcggctt gcaccagcag cggaaacaat
     4621 aatattgcta ataaggttct cattatttgc cgccaattgt cgccgtcatg cggatatgtc
     4681 ggttatcaga cagttccaga ttcgacagga ccactaactg cggcaagctg cggcgcagga
     4741 agcgagacaa taatggtcgc agcgcgtggt tcaccaacaa tactggcggc gcgcccagca
     4801 tctcctgacg ggatagcgct tcctgagttt gcgccagtaa acgatccgcc agccctggct
     4861 ccagtcctcc cccgccctgc agcgcctgta gtaacaaacg ttccagcggt gtatcgaggc
     4921 caataacatg gacttcatct ttgccaggaa accactgctg ggtaatcgcc cgtcccaacg
     4981 ccacgcgcac gacggcggtt aattcatgtg gatcgctttg gatgggcgca tgttccgcca
     5041 gcgtttcgag aatggtgcgc atatcgcgaa tcggtacttt ttcatcgagg agattttgca
     5101 gcactttatg cagtgtggtg agcgtgacga cgccaggaac gagatcttcc gtcagctttg
     5161 gcatctcctg ggcgacgcga tccaacagct gttgcgcctc ctgacgacca aacagctctg
     5221 cggcatgctg gctaatgagg tggttaagat gcgttgctac caccgtgctg gcctcaacca
     5281 ctgtgtaccc ctgaatctgc gcctgttctt ttagcgcact ttcaatccag atagcattca
     5341 ggccaaatgc cggatcgacg gtcgcctcac caggtaacgt cccggcagcg gttccagggt
     5401 taatcgccag ccagcgcccc ggataagcat caccactgcc aatctccacg cctttcatca
     5461 aaatgcgata gcgggcaggt tgcagatcca tattgtcgcg aatgtgcacc actggcggca
     5521 gaaatcccat ctcctgggca aatttcttgc ggatactgcg tatacggccc aacaactcac
     5581 catcctgctg gaaatcgacc atcgggatca gtcgataacc cacttccatt cccagagaat
     5641 cttccagttg tacatcgttc cacgtcgctt cgacaacggt attattctct gccattttta
     5701 ccggtttggg ttcggcaggc gctttttgtt cgcgtccgcg tatccaccag gccagcccga
     5761 gcaatccggc agtgaacagc aaaaatacca ggttcggcat tccaggcacc aggccgagta
     5821 aaccgagcac ggcggcgctt aacaacataa cgcttgggtt actgaaaagc tgattcacca
     5881 tctgctcgcc aacatcctga tcggtgctga cacgcgtaac gatgaccccc gcggcggtag
     5941 aaatcaccag cgccggaatt tgtgccacca gaccgtcgcc aatggtcaat agcgtataac
     6001 tttccgccgc gtgtcccatg ctcatgccat gttgcagcac gccgaccagc aacccgccga
     6061 caacgttaat gaccatgatg aggatcccgg cgatggcatc gccgcgaaca aacttacttg
     6121 ccccgtccat tgagccgtaa aaatcggctt cctgagtcac ttcggagcgg cgttttttcg
     6181 cctcatcttc accaatcaat ccagcgttaa ggtcggcgtc aatcgccatc tgcttacccg
     6241 gcataccatc gagaacaaag cgcgcaccca cttctgcgat acgcccggca cctttggtaa
     6301 tgaccataaa gttgatgatc acgagaatga caaacaccac gataccgata gcgaaattgc
     6361 caccaacgag gaagtgaccg aacgcttcga ccaccttccc tgccgccgcc gcgccggtat
     6421 gcccttccat caaaatgata cgggttgaag ccacgttaag tgccagacgc aacaacgtag
     6481 taaacaacag aatggtcgga aacgcagcaa actcaagcgt gcgctgggta aacatcgcca
     6541 ccagcaacac catgatcgac aaggcaatat tgaaggtaaa caacaggtcg agtatgaatg
     6601 cgggcagtgg cagcaccatc atcgacaaga tcaacaggat caaaatcggt ccggcaagga
     6661 tctgccattg tgtcgatttc aggtttgcgg gcaggcgcag catcgcggcc agattactca
     6721 tgggtcggtt tctcgttaat aaaatccagg gcttccggca ccggaagatg agtaggttgt
     6781 acagggcgct gtccaccagc cagacgccag cgtttcagtt gccagaccca ggccagcact
     6841 tccgccaccg cggcgtacag ttgacccggg atttgttgac caatctccgc atgtcgatac
     6901 agcgctcgcg ccagcggcgg cgcttcaagc gtcgggacgt tattttcagc gccaatttca
     6961 cgaatgcgca gcgcgaccag ccctgcacct ttagcgacca ctttcggtgc gctcattttg
     7021 ttttcgtcat actgcaacgc taccgaatag tgggtcgggt tattgacaat gacatcagct
     7081 ttcggcacat cggccatcat ccgacgccgt gcagcagctc gctgcatctg acggatccgc
     7141 cctttaacat gagggtcacc ttcgctttgt ttgaactcat cacgaatatc ctgccgcgac
     7201 atacgcagct ttttcaggtg gctgaagatt tggaaaaaga cgtcaaatcc caccattgga
     7261 atgacaccaa gcaccaccag cagtgcgcat agcccaacca gatccatcgc attacccatg
     7321 gcggtagtcg gagactcggc cattaagcgc atcatctgcg gccagtgatg ccagagataa
     7381 aaccccgtca cgctgccaac caggatggtt ttcaaaattg ccttaagcaa ctccgcgcca
     7441 gtctgtgccg agaacatccg tttaatgccc ggtagcgggt tgagtttgga aaacttcggc
     7501 tgcaaggatt tgccgctaaa taccagcccc cccagcatga ccggagaaat gagcgccacc
     7561 agcaccacgc cgctaatcag cggcagcagc gccagcatgg cttctctgat cagcagaata
     7621 atctgcccga ggatcagatt cgggtca
//
LOCUS       DAZPEP010000185         7612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZPEP010000185 DAZPEP010000000
VERSION     DAZPEP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.186"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            172..702
                     /locus_tag="ACHUT9_003798"
     CDS             172..702
                     /locus_tag="ACHUT9_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1350176.1"
                     /translation="MNDKELIKEIKERISSLDVRDNVERRAYEIALASLTAEPVAWKV
                     TFTQIDREYNTFTGMYSDKAEVERWVRLHKACNFRADITPLYTAQPVPVTPDAWISCS
                     ERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGNPTWDDGDGEDLYLKEV
                     THWMQLPEPPQEVNRG"
     gene            838..1503
                     /locus_tag="ACHUT9_003799"
     CDS             838..1503
                     /locus_tag="ACHUT9_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350177.1"
                     /translation="MLLKMVNEARKLYGEPSVRNNKFIEKIEDELEGETYTKSVGRKN
                     GADIDVISMTIKQALRVAARESKAVRRTLVDKLESMQEAHIKSGKSASGLVEYRQART
                     LKMTVEAVTNLFDLMPNLAPEAKQTAAASIINPLVGFNAIPLPAIEEHYYSAGEVAEQ
                     LGVTANKIGRIANANNLKTEQYGKFFLDKSAHSSKQVETFRYNEAGVHKIEELIEGER
                     KAA"
     gene            1825..2448
                     /locus_tag="ACHUT9_003800"
     CDS             1825..2448
                     /locus_tag="ACHUT9_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFR1350178.1"
                     /translation="MASQLIPVFNGTISNETTLLVNARDLHEFLEVRRDFSTWIKNRI
                     TEYGFAVDVDYILVHQNRGIKGRGGDRRSKDYHLTLDTAKETAMVERNEKGRQIRRYF
                     IECEKKLRSMQPAQQFTDEEIILLCYMQVQMENAQDICKRLYPILKELNSSYTSKLYD
                     IAFETFYTVTKNRDVLLREATRLDQASALFERAKPMLKSLRARQFEF"
     gene            2533..2751
                     /locus_tag="ACHUT9_003801"
     CDS             2533..2751
                     /locus_tag="ACHUT9_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1350179.1"
                     /translation="MYLTLQEWNARQRRPRSLETVRRWVRECRIFPPPVKDGREYLFH
                     ESAVKVDLNRPVTGSLLKRIRNGKKAKS"
     gene            2729..3799
                     /locus_tag="ACHUT9_003802"
     CDS             2729..3799
                     /locus_tag="ACHUT9_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1350180.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEAIQANIELFSGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMS
                     KIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHI
                     TTKPVAATRAAKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCE
                     MKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTR
                     REPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHK
                     SDTMASQYRDDRGREWDKIEIK"
     gene            3934..5217
                     /locus_tag="ACHUT9_003803"
     CDS             3934..5217
                     /locus_tag="ACHUT9_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFR1350181.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(5451..>7612)
                     /locus_tag="ACHUT9_003804"
     CDS             complement(5451..>7612)
                     /locus_tag="ACHUT9_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFR1350182.1"
                     /translation="GTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGIVQTAKAS
                     GMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMRE
                     MMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKP
                     APYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSS
                     VWQTDEEVHNWLALHGRGQDYCQLNPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYE
                     IDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENV
                     IAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFG
                     AGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGGYLTSASE
                     LDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTDNIVLKVC
                     SKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAELENQRLAG
                     NVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQRVIGGLAN
                     IAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIH
                     EDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
BASE COUNT         2093 a         1863 c         1897 g         1759 t
ORIGIN      
        1 gcgctacgcg ataagcaggc gttacttgaa gcctcagaaa agcgcatagc agaactggag
       61 gcacagacag ttaccgttaa agaggttgga gatgcttgat agttttgcgc tcgttgcact
      121 ggttggcatt gtagcgttga tttgggtgac atccgggaat cggaggcata agtgaacgac
      181 aaagaactga ttaaagaaat caaagagcgt atcagcagtt tggatgtgcg agacaatgtt
      241 gaacgccgtg cttatgaaat tgctctggca tcgctgacag cagagccggt ggcgtggaag
      301 gtaaccttca cgcaaattga ccgtgaatat aacacgttca ctggtatgta ttctgacaaa
      361 gcagaagtcg aacggtgggt gcggctgcat aaagcatgta attttcgggc agatataaca
      421 ccgctttata ccgcccagcc agtgccggta actccggatg cctggataag ctgtagtgag
      481 cgaatgccag aaatgggaga gcgacaatgc tatgtgttag cagctgactt taaaaacaac
      541 tacccaccaa acatccccaa cactcaggtc ggcgtatatg gcgactggtt taatgatggc
      601 aatcccactt gggatgacgg tgatggcgaa gacctgtatc tcaaagaggt aacccactgg
      661 atgcagctac cagaaccgcc gcaggaggtt aaccgtggct aacctgcaac ttgccgtcaa
      721 aggtgaataa caatcctcgc actcgcgggg atttctttta tacgatatag gggaaaaggt
      781 gtggttaaca atattgcaaa tcactcttcg tttccggctg taacaatcga cagccagatg
      841 ctgttgaaaa tggtcaatga agctcgcaag ttatatggag agccatcagt acgtaacaac
      901 aaattcatcg agaaaattga ggatgaattg gaaggcgaga cctacacaaa aagtgtaggt
      961 cggaaaaacg gggctgacat tgatgttatc tccatgacta tcaagcaggc gcttcgtgtt
     1021 gctgctcgcg aatctaaagc agttcgccga acacttgtag acaaacttga aagtatgcag
     1081 gaagcgcaca ttaaaagcgg taaatcagcg agtggacttg ttgagtatcg tcaagcgcga
     1141 acactgaaaa tgacggttga agctgttacc aatctgttcg atttgatgcc aaatcttgcg
     1201 ccggaagcaa agcagactgc tgcggcaagc ataatcaacc cgctcgttgg ttttaatgca
     1261 atacctcttc cggcaataga agagcattac tactcagcag gggaggtcgc agagcagctc
     1321 ggagtaacgg caaacaagat tggtcgcatt gctaacgcaa acaacctcaa aactgagcag
     1381 tacgggaagt tcttcctgga taaatctgcg cattccagca agcaggttga aacatttcgg
     1441 tacaacgagg ctggagtgca caaaatagag gaactcatcg aaggagagcg aaaggctgca
     1501 tgattttgac aagatagttt tccccaaatg tggggaaaag cccgaatggc gcggcttaca
     1561 gcaagataag gcctacatga tttgacaaca ccgcattaac ggggctatat tccgcttcat
     1621 ggtgctgaac acaccttgca aagcggaaac cgcacccgtc agtcatgcgg attttttatg
     1681 tccatttttc agatatggtc gggtagcgcg tataccgaaa aacagccgaa aggttaagga
     1741 tacgggccga ctttgcacgg tgttcaagta cctgaccgcc ctgctgaaca cagggctatc
     1801 tgaacaaatg caaaggacat aaaaatggcc agtcaactca tccccgtatt caacggcact
     1861 atatccaacg aaacaactct tctcgttaat gcccgtgatt tacacgaatt tttggaagta
     1921 cgtcgtgact tctctacatg gattaaaaat cgcataacag aatacggctt tgccgttgat
     1981 gtcgattaca ttttggttca ccaaaacagg gggatcaaag gacgtggagg cgatcgccgt
     2041 agcaaagact atcacctcac cctcgataca gccaaagaaa cggcgatggt cgagcgtaac
     2101 gaaaaaggcc gccagatacg ccgatacttc atcgagtgcg aaaagaaact tcgcagcatg
     2161 caaccagcgc agcaattcac agacgaagaa atcatcctcc tttgctatat gcaggtacag
     2221 atggagaatg cgcaggacat ctgcaaacgt ctgtacccga tattgaagga actgaactca
     2281 tcatacacga gcaagctgta tgacatcgcg tttgaaactt tttacacggt gacgaaaaac
     2341 agagatgtac tgctaaggga ggcgacacga cttgaccaag caagcgccct tttcgaacgg
     2401 gcaaaaccaa tgctgaaaag ccttcgggcg agacaattcg aattttaatc atcaaaggag
     2461 cttcggctcc ttttttgttg gagaaaataa accaatactc gctcccttgc gagtaattgc
     2521 ggagactttg cgatgtactt gacacttcag gagtggaacg cacgccagcg acgcccaagg
     2581 agccttgaaa cagttcgtcg atgggtacgc gagtgcagga tattccctcc tccggttaag
     2641 gatggaagag agtatttgtt ccacgaatca gcggtaaagg ttgacttaaa tcgaccagta
     2701 acaggtagcc ttttgaagag gatcagaaat gggaagaagg cgaagtcatg agcgccggga
     2761 tttaccccct aacctttata taagaaacaa tggatattac tgctacaggg acccaaggac
     2821 gggtaaagag tttggattag gccgagacag gcgaatcgca atcactgaag ctatacaggc
     2881 caacattgag ttattttcag gacacaaaca caagcctctg acagcgagaa tcaacagtga
     2941 taattctgtt acgttacatt catggcttga tcgctacgaa aaaatcctcg ccagcagagg
     3001 aatcaagcag aagacactca taaattacat gagcaaaatt aaagcaataa ggagggggct
     3061 gcctgatgct ccacttgaag acatcaccac aaaagaaatt gcggcaatgc tcaatggata
     3121 catagacgag ggcaaggcgg catcagccaa gttaatcaga tcaacactga gcgatgcatt
     3181 ccgagaggca atagctgaag gccatataac aacaaaaccg gtcgctgcca ctcgcgcagc
     3241 aaaatcagag gtaaggagat caagacttac ggctgacgaa tacctgaaaa tttatcaaac
     3301 agcagaatca tcaccatgtt ggctcagact tgcaatggaa ctggctgttg ttaccgggca
     3361 acgagttggt gatttatgcg aaatgaagtg gtctgatatc gtagatggat atctttatgt
     3421 cgagcaaagc aaaacaggcg taaaaattgc catccctaca acattgcatg ttgatgctct
     3481 cgggatatca atgaaggaaa cacttgataa atgcaaagag attcttggcg gagaaaccat
     3541 aattgcatct actcgtcgtg aaccgctttc atccggcaca gtatcaaggt attttatgcg
     3601 cgcacgaaaa gcatcaggtc tttccttcga aggggatcca cctacctttc acgagttgcg
     3661 cagtttgtct gcaagactct atgagaagca gataagcgat aagtttgctc aacatcttct
     3721 cgggcacaag tcggacacca tggcatcaca gtatcgtgat gacagaggca gggagtggga
     3781 caaaattgaa atcaaataat gattttattt tgactgataa tgacctgttc gttgcaacaa
     3841 attgataagc gatgcttttt tataatgcca acttagtata aaaaagcagg cttcaacgga
     3901 ttcatttttc tatttcatag cccggagcaa cctgtgaaca cattttcagt ttcccgtctg
     3961 gcgctggcat tggcttttgg cgtgacgctg accgcctgta gctcaacccc gcccgatcaa
     4021 cgtccttctg atcaaaccgc gcctggtacc tcttctcgcc cgattctgtc ggcaaaagaa
     4081 gcgcagaatt tcgatgctca acactatttt gcatccctga caccaggtgc tgcagcgtgg
     4141 aatccttccc cgattaccct gcctgcgcaa cctgactttg ttgtcggccc ggcgggcact
     4201 caaggtgtaa cgcataccac gattcaggcg gcggtagatg cggcaattat caagcgtacc
     4261 aacaagcgcc agtatattgc cgtgatgcct ggtgagtatc agggaacggt atatgtccct
     4321 gccgctccgg gtggaattac tctgtacggt acaggtgaaa aaccgattga tgtgaagatt
     4381 gggctttccc ttgatggtgg catgagccct gccgactggc gtcacgacgt caacccgcgc
     4441 ggcaaatata tgccaggtaa accagcgtgg tatatgtacg atagctgcca gagcaaacgc
     4501 agcgacagta tcggtgttct ctgctctgcg gtcttctggt cacaaaacaa tggcctgcaa
     4561 ctgcaaaatc tgaccatcga aaacacgctg ggcgatagcg tagatgcagg taaccatccg
     4621 gcggtggcac tgcgtactga tggtgaccag gtacagatta acaacgttaa cattctcggt
     4681 cgtcagaaca ccttctttgt caccaacagc ggtgtgcaga accgtctgga aacgaatcgt
     4741 cagccgcgta cgctggtgac caacagctac attgaagggg atgtggatat cgtttctggt
     4801 cgcggcgcag tggtgttcga taacaccgaa ttccgcgtgg tgaactcacg tactcagcaa
     4861 gaagcgtatg tgtttgcacc ggctacgctg tccaacatgt actacggttt cctcgccgta
     4921 aacagccgtt tcaatgcttt cggtgatggt gtggcgcaac tgggccgctc gctggatgtt
     4981 gatgccaata ccaacggtca ggtggtgatc cgtgatagcg ccatcaacga aggttttaac
     5041 acggctaaac cgtgggccga tgcggtgatc tctaatcgtc cgtttgcggg taataccggc
     5101 agcgtagatg ataacgacga aatacagcgc aatctgaatg acactaacta caaccgcatg
     5161 tgggaataca ataaccgcgg cgtgggtagt aaagtggttg cagaggcgaa gaagtaagag
     5221 caattaacta tttgccggat gcggcgtaaa cgccttatcc ggcctacggt tcgatgcgat
     5281 ttgtaggtcg gataagatgc gcaagcatcg catccgacaa taagtgccgg atgctgcgta
     5341 aatgccttat ctggcctaca gattcgatgc gattcgtagg tcggataaga tgcgcaagca
     5401 tcgcatccga caataagtgc cgaatgcgac ctacattcac atggcgcttt ttacatctga
     5461 cggtttttat tgaagttaat caaactaccc gccttgataa tctcgcgctc ttcagcagtc
     5521 agactttcca tatagagcgt aatttccgtt accggcgcat cttcatggat cacataacct
     5581 ttaaacgtcg tacccggatt atccagcgcc gctttaatgc cagggatgta aatgtaatcc
     5641 cccacttcaa aggttggcac ttccgccatc tgcagaggta acatccccca gttgatgacg
     5701 ttagaacggt agcgtttagt cgcgtactcc tcggcaatat tcgccagacc gccaatcaca
     5761 cgctggcagc tcgccgcctg ttcacgcgca gaaccatcgc ctggtttcac cgcatagacc
     5821 atgctgccaa tttcagtttg cagcggatca atatgctcct gaccagcaat ctgcttaatg
     5881 cgcgcaaaca cctctgtcag ctcgctgaca ttccccgcca gacgctgatt ttccagctca
     5941 gcagtcgctt tacttctgcc aacataaccg ggatcacggc gagacagggt aaactccgcc
     6001 agaccaatcg gatttgaacg ataagaagag gtttcaccgg aaggaatcag ttcgtcggtg
     6061 gtggtcactt cgtcgaggat cttcgagcac actttcagga cgatattgtc agtcagcgca
     6121 cccaattccg gccagtcttt aatgttcggc ccgtaaatca gcggttgctg agttgcccct
     6181 ttcacaaagc cctgataaac acggttttta tacggcgtta catcgaaggc gtactccggc
     6241 acgttgtccc agcaatcaag ttcgctggca gaggttaaat agccaccgtt tgccgcagtc
     6301 gcagcgatag aacgagcgtc catcaacgcc accgctgaca tctgcccatt agctggctta
     6361 gagccttcgc ggttcgggaa gttacgcgtg gtgtggcgaa tactcaaacc gttgttgatt
     6421 ggcgtatcgc ccgcgccaaa gcatgggccg cagaacgcgg ttctgatgat tgcgcctgcg
     6481 ccaatcaaat ctgctaccac accttttttg gcgagatcca taaacaccgg ctgtgatgac
     6541 gggtaaactg ccagcgagaa ggtgtcattg ccacaggatt gaccgcgcag tgcattcgcc
     6601 gccgcgatga cgttttcgta gttaccgcca gaacagcccg cgataatccc ctgctgcact
     6661 ttcaggcgac cattttccac tttatccagc agcgagagtt tggctttacc gtgcgccacg
     6721 cgttcggact caatttcaat ctcacgcaga atgtcggtca ggttctggtt cagtgtgtcg
     6781 atttcataca cgttgctcgg gtggaacggc agcgcaatca ttggtttgat ggcgcttaaa
     6841 tcaacgctga tgcagccatc gtagtacgcc atcggttgag ggttaagctg gcagtaatcc
     6901 tggccgcgac cgtgcagcgc cagccagtta tgcacttctt catcagtttg ccagacggag
     6961 cttaaacagg tggtttccgt ggtcatcacg tcaacgctgt tacggaaatc ggtagagagc
     7021 gcgctaacgc ccggtccaac gaactccatg actttgtttt tgacgtaacc gtttttgaac
     7081 accgcgccaa tgatagccag cgccacatcc tgtggcccca catacggcgc aggttttccg
     7141 gtcagatgca ccgcaaccac gcccggatag tcgatatccc aggtgtcatt aagcagctgt
     7201 tttaccaact caccgccacc ctcaccgact gccattgtcc ctaatgcacc gtaacgggtg
     7261 tggctgtctg acccgaggat cattttgccc ccgcctgcca tcatctcacg catatattga
     7321 tggatgaccg caatatgcgg aggcacaaaa ataccgccat aacgctgagc tgccgataaa
     7381 ccaaaaacat ggtcatcacc gttaatggtg ccgccgacgg cgcagagtga gttatggcag
     7441 ttggtcagca catacggcag cgggaaacgt tccattccgg acgctttagc ggtctgtaca
     7501 ataccaacaa aggtaatatc gtgagaggct aatgaatcaa acttaatttt aagtttatcc
     7561 atatttccgg acgtattatg agaagagaga atagaccagg caatagtgcc tt
//
LOCUS       DAZPEP010000186         7491 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZPEP010000186 DAZPEP010000000
VERSION     DAZPEP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.187"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUT9_003805"
     CDS             complement(16..522)
                     /gene="mug"
                     /locus_tag="ACHUT9_003805"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFR1350183.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            647..722
                     /locus_tag="ACHUT9_003806"
     tRNA            647..722
                     /locus_tag="ACHUT9_003806"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:680..682,aa:Ile,seq:cat)
     gene            complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUT9_003807"
                     /gene_synonym="yqjH"
     CDS             complement(776..1540)
                     /gene="nfeF"
                     /locus_tag="ACHUT9_003807"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFR1350184.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUT9_003808"
                     /gene_synonym="yqjI"
     CDS             1828..2451
                     /gene="nfeR"
                     /locus_tag="ACHUT9_003808"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFR1350185.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUT9_003809"
     CDS             complement(2558..4078)
                     /gene="aer"
                     /locus_tag="ACHUT9_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFR1350186.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUT9_003810"
     CDS             4496..5875
                     /gene="ygjG"
                     /locus_tag="ACHUT9_003810"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFR1350187.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5917..6249)
                     /locus_tag="ACHUT9_003811"
     CDS             complement(5917..6249)
                     /locus_tag="ACHUT9_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFR1350188.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUT9_003812"
     CDS             6468..7451
                     /gene="ebgR"
                     /locus_tag="ACHUT9_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFR1350189.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1823 a         2031 c         1869 g         1768 t
ORIGIN      
        1 tttcggtggc gttttttatc gcccacgcac taccagcgcc tggtccagct cgcgatacgc
       61 ttcaaccagt ttctctagtg aaacgcgact taaaccgctg ggatttggca gcacccaaat
      121 ctgcgtcgaa ccaatggtga gcgtttgttt cccccactgt gcaccgcgct ggctgaatcc
      181 ctgttcatat gcttgtttgc ccagaatcgc caacgcctgc ggctgataat cttcaatttt
      241 ttcaatcagc ttacgcccac ctgcgtgcag ctcctgcttt gaaacttcat tggcttgcac
      301 cgttggacgg tctaccagtt tggtgacgcc acaacgataa tccagcagat gctgtgcctc
      361 ctgcggcttc aactgacggt cggtaaaccc ggcctgatat atcaccttcc agaagcgatt
      421 tgctggatga gcaaagggaa aaccagtccc ggcggatgaa agcccagggt tgataccgca
      481 aaacacgacc cgtaaccctg gagccaaaat atcctcaacc atatttactc ctttctaaca
      541 tcaccaggaa aagtataacg gattgaaaat acattgttta taaaaacagc aggcgcacgg
      601 taatggctgg attgcgacac ggagttactt tataatccgc taccatggcc ccttagctca
      661 gtggttagag caggcgactc ataatcgctt ggtcgctggt tcaagtccag caggggccac
      721 cagatatagc aaaggctgac gagaaatcgt cagccttttt ctttttatat atcagttact
      781 ttgcgtgcca gtaagccgct gcacgtaccc gctgtgggtc atactgttcc gcttcaaagc
      841 ggcggcttaa attcttaacg actttacctt cgccggttat ccagatgaag taatcatcgg
      901 cagggatttg catctgcgcc agacgcgcat ctaccgcctg ctcatcatgt gccagccatt
      961 cgatattaaa accatcaagg tgcgcgagat aatcctgaca ggcgttatcc cgcacgctaa
     1021 ccagcgcact aacttgcggt ttaacggcaa gtttgctcaa cgtttccaag cggcggcgca
     1081 gtgcgggcat tccggattca tcgcagacat acagctgata cgcgtaatct tccggcacca
     1141 ccagcgaacc gcgcggacct gcaaccgtaa gtttatcgcc cggttgcgcc tgcatcgccc
     1201 agccgctggc gaccccaccg tcgtgaataa agaaatcgat cgccagttca tggcgtagtt
     1261 cgtcatacag cggcgtatag tcacgcgacg gtgggcgtgg tccttccggc cagacgatgc
     1321 cctcttccgt taccgttggc ggcacaaagt gagcgtcagg ttgaggaaag aagagtttgc
     1381 tgtgatcgtc aaagccgcgc gatgtaaaac cgtccagcgc ctcgccgccg aggacaatgc
     1441 gctgaaaacc ggcgctgatg cgctcaacgc gtaacacagt cagttcacgg aagcgcagat
     1501 cattgcgaac gcgctgcggg tagcgggggg agttattcat tgttatcgcc ttcgtgatgg
     1561 taatcagata tatctaaata attttcgtaa atgataatga ttgttaatca tgataaatgc
     1621 aagcgatttg tagaactgat atgtctatag tctgataaga cgaaccgcct cttctcaggc
     1681 atcattactc aacgccggat gcggcgtgaa cgccttatcc ggcctacgtg tgagatgagt
     1741 ccacatttaa aaatcatttt tattcttgca agagtgctca tatcagatat aaattagata
     1801 tatctaatca agcaaaagga ggctgatatg agccatcatc acgaagggtg ttgtaaacat
     1861 gaaggccagc cacgccatga gggctgctgc aaaggtgaga agtcagaaca cgagcactgc
     1921 ggacacggtc accagcatga acacggtcaa tgctgcggtg gtcgccacgg tcgcggcggc
     1981 ggtcgtcggc aacgtttctt tggtcacggt gaattgcgtc tggtgattct ggatattctc
     2041 tcgcgcgatg acagccacgg ttacgaattg attaaagcga ttgagaatct aacccagggg
     2101 aattacaccc caagcccggg cgtcatctac ccaacgctgg attttctgca ggagcagtcg
     2161 ctgattacca tccgcgaaga ggaaggtggt aagaagcaga ttgcgctgac cgaacaaggc
     2221 gcgcagtggc tggaagaaaa ccgcgaacag gtggagatga ttgaagaacg catcaaagcg
     2281 cgttgcgttg gtgctgcgct gcgccagaac ccgcaaatga agcgggcgct ggataatttt
     2341 aaagcggtgc tggatttacg cgtcaaccag agcgatatca ctgatgcaca aataaaaaag
     2401 atcattgcgg tgatcgacca cgccgctttt gatattaccc aactggatta atcaccgatt
     2461 gccggatgcg gcgtacccta cacaccccgc aggcctgata agatgcgcca gcatcgcatc
     2521 aggcattgtg ctccaaccgc cggatccggc ataccgatta atgcagcacc gtcaccgcgt
     2581 cttccagtcg gctggcgcgg tgtttcacca tcgccgacac ctgcgcactc tcttccacca
     2641 gctcagcatt tttctgggtg atcaggttaa gctcatccac tgcacgggtc aggctggaaa
     2701 gcccatcggc ctgttccagc gttgaatggc taatctgggc gatcaactgg gtgacgtttt
     2761 tcacctgtgc cacaatatct tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg
     2821 attgcacctt atcagcactg gcatcaatca gcttgcgaat atcgttggca gcattagcgc
     2881 tgcggctggc taaatgacgc acttcccctg ccaccactgc aaaacctttg ccctgttcgc
     2941 cggcacgcgc cgcttccacc gccgcattca gggccagaat attggtctga aacgcaatat
     3001 cgttaatcag cgaagtaatg gtgccaatgc gctgggtact gtcggcgata tcgtccattg
     3061 tcttgatcac cgtggtcatc gcctccccac cctgcactgc cgcattactg gcggtgatag
     3121 aaagtttatc ggcagccgac gccgtggcag agttctgttt caccgacgcc gccatttggt
     3181 tcatggtcgc caccgtttgc tgaacgttat caactgtctg ctgggtatgt tcgttcagtt
     3241 catcggtgcc tttcgccagc gtctcactgc catttctgac actggacacc tggcttgaga
     3301 catcgttaat tagccaacgg cacatcaggc caagctgccc taccgcgcgt aatgtcagcc
     3361 ccagctcatc gctgcgattc aggtgctcaa cactattacg ctctccggtc gccaccttca
     3421 gtgcctgacg ggcgacattt tctatcgggc gcacaatctg ccattcaaaa caagcgcttg
     3481 ccaacaatac cactaacaca cagaggaaat acgtcaccac cggggcagca acaaaccaaa
     3541 gcatggccgc cagcaagata aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg
     3601 gtaatgaagg cagtttaccc agccagcctt tacgcaccac caggccttta tgaatacgct
     3661 tactggtacg tccggcgttc agcgctttgt acagcggctc caccgccgca atctcttcat
     3721 ccgtcgcccg ggtacgaatc gacatatagc cactgatttt tccctcgcgc accatcggta
     3781 ccgcattggc ccgcacccaa taatggtcgc catttttgcg gcgatttttc acgatgccgc
     3841 tccagggctc cccttttttc agggtgaacc acatatccgc aaacgccgct tttggcatat
     3901 ccgggtgacg caccatgttg tgcggctgcc cttgtaactc ttgcaaggta aagccgctca
     3961 cctgcacaaa agtgtcatta gcatgagtga tatagctttg cagatcggta gtggacatca
     4021 gagtggtatc gtccgccagc ggggtatttt gctgggtgac atacggatga gaagacatgt
     4081 tcgcgtcctg tgcaggttat atggttgtta acttcttgtc agagtttatg tcggccccgc
     4141 tgcggttatc tttaaccgat taatttgatt tagatcgcaa tttgcgatta aagcacaaat
     4201 ctaattcttt gatttaaaat actttcactc tgttactata cgaaaacgtt aattatcttg
     4261 cccaaaaatc aggcaattat tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc
     4321 atacccgaca aaaaccgtgc aaaataacaa caaatgttaa cagatagcat taaatattgc
     4381 acaaatgata accgaatttg tgtttatccc gattttcgcg atcgcagccg gagtggcgca
     4441 atccctgcaa tacttaaatc ggtatcatgt gatacgcgag cctccggagc atattttgaa
     4501 caggttacct tcgagcgcat cggctttagc gtgcagcgcc cacgccctga atctcattga
     4561 gaagcgaacg ctggatcatg aggagatgaa agcacttaac cgagaggtga ttgaatactt
     4621 caaagagcat gtcaatccgg ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga
     4681 ttacggagcc gtagagtggc aagcgggaag tttaaatacg cttgtcgaca cccagggaca
     4741 ggagtttatc gactgcctgg gaggttttgg aattttcaac gtggggcacc gtaatccagt
     4801 tgtggtttcc gccgtacaga atcaacttgc gaaacaaccg ctgcacagcc aggagctgct
     4861 cgatccgtta cgggcgatgt tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa
     4921 atacagcttc ttctgtaata gcggcaccga gtccgttgaa gcagcgctga agctggcgaa
     4981 agcttaccag tcaccgcgcg gcaagtttac ttttattgcc accagcggcg cgttccacgg
     5041 taaatcactt ggcgcgctgt cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc
     5101 gttactgccg ggcttccgtc atgtgccgtt tggcaatatc gaagccatgc gcacggctct
     5161 taacgagtgc aaaaaaaccg gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg
     5221 tgaaggtggc gtaattctgc cgccgccggg ctatctcacc gccgtacgta agctatgcga
     5281 tgagttcggc gcactgatga tcctcgatga agtacaaacg ggcatggggc gcacgggcaa
     5341 gatgttcgcc tgcgagcatg agaacgtaca gccggatatc ctctgccttg ccaaagcgct
     5401 cggcggcggc gtgatgccga ttggcgcgac catcgccact gaagaggtgt tttcagttct
     5461 gttcgacaac ccattcctgc ataccaccac ctttggcggc aacccgctgg cctgtgcggc
     5521 ggcgctggcg accatcaatg tgttgctgga gcagaactta ccggctcagg ctgatcaaaa
     5581 aggcgatatg ttgctggacg gtttccgtca actggcgcgg gaatatcccg atctggtaca
     5641 ggaagcgcgt ggtaaaggga tgttgatggc gattgagttt gttgataacg aaatcggcta
     5701 taactttgcc agcgagatgt tccgccagcg cgtactggtg gccggaacgc tcaataacgc
     5761 caaaacgatc cgcattgaac cgccactgac actgaccatt gaacagtgtg aactggtgat
     5821 caaagcggcg cgtaaggcgc tggcggccat gcgagtaagt gtcgaagaag cgtaatatca
     5881 aatcggatgg cgatacgacg tcgccatccg atctttttat acgacgcgta cgcccgcagg
     5941 catcatccgc tccggcgtta acagcacgct ttcactgccg tcatcggttt ccgcacacaa
     6001 cagcatgcat tccgacgttt caccacgcat tttcgctttt tgcagattgc ataagaccac
     6061 caccgttttt cccatcagct cctcttcgct gtagtacggc accaggctgg tcacggtttg
     6121 cagtgttttt tcccccacat caacctgtac gatgtacagc ttgtcggcgt tttcatggcg
     6181 tttcacttcc acaatctttc cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac
     6241 ggtttccatt gcttcctccc ttcaagtaaa attttactaa actatagcaa aattttttct
     6301 caatcctgta ggctaaaaat ggagaatgca ggcgtgatca cattcgtaag ccgctgtgtt
     6361 accgttacag cgtcaaagaa acgcgcttta tttactgaaa acaggtgacc cgataagcac
     6421 ttcctctaca atgggggcgc acatcaggga aagtaaaaaa ggtaaacatg gcaacattaa
     6481 aagacatcgc aatcgaagct ggcgtatccc tggcgacagt atccagggtc ttaaatgacg
     6541 atccgacatt gaatgtgaaa gaagagacga aacatcgcat tctcgagatc gccgaaaagc
     6601 tggagtacaa gaccagtagc gcccgtaaac tacagacagg cgcagtcaac caacaccata
     6661 ttctggctat ctacagctac cagcaggagc tggagatcaa cgatccttac tatctggcga
     6721 tccgccacgg cattgaaact cagtgcgaaa agctggcgat cgaactgacc aactgttatg
     6781 aacacaacgg attaccagac attaaaaacg tcaccggtat tttaattgtc ggcaaaccca
     6841 cgcccgccct gcgcgccgct gccagcgcgt tgaccgacaa tatctgtttt atcgactttc
     6901 acgaacccgg cagcggttac gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa
     6961 tcatcgactt ctatatcaac cagggcgtta atcgtattgg ttttattggc ggtgaagatg
     7021 agcctggcaa ggcggatatt cgtgaggtcg cctttgcgga atatggccga ctgaaacaag
     7081 tggtacgcga agaggatatc tggcgcggcg gtttttccag ttcgtcgggt tatgaactgg
     7141 caaaacaaat gctggcgcgg gaagactatc cgaaggcact gtttgttgct tccgattcca
     7201 ttgctatcgg cgtactgcgg gcaattcatg aacgtggcct gaacatccca caggatattt
     7261 cgcttttcag cgttaacgat atccccaccg cgcgatttac ctttccgccg ctctccaccg
     7321 tgcgcatcca ttccgagatg atgggaagtc agggcgttaa cctggtgtat gaaaaagccc
     7381 gcgatggtcg cgcgctgccg ctgctagtct tcgttcccag caaattaaaa ctgcgcggca
     7441 cgacccgtta aatcccctta cacactgtcc ggcaatcatt tttgccggac a
//
LOCUS       DAZPEP010000187         7477 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZPEP010000187 DAZPEP010000000
VERSION     DAZPEP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7477
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.188"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            38..250
                     /locus_tag="ACHUT9_003813"
     CDS             38..250
                     /locus_tag="ACHUT9_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350190.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="ACHUT9_003814"
     CDS             283..501
                     /locus_tag="ACHUT9_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1350191.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            572..889
                     /locus_tag="ACHUT9_003815"
                     /pseudo
     CDS             572..889
                     /locus_tag="ACHUT9_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUT9_003816"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="ACHUT9_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFR1350192.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="ACHUT9_003817"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="ACHUT9_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="ACHUT9_003818"
     CDS             1917..2660
                     /locus_tag="ACHUT9_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFR1350193.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="ACHUT9_003819"
     CDS             2690..3229
                     /locus_tag="ACHUT9_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFR1350194.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUT9_003820"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="ACHUT9_003820"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFR1350195.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUT9_003821"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="ACHUT9_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFR1350196.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="ACHUT9_003822"
     CDS             4582..6102
                     /locus_tag="ACHUT9_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1350197.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..6905)
                     /locus_tag="ACHUT9_003823"
     CDS             complement(6135..6905)
                     /locus_tag="ACHUT9_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFR1350198.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHTIANGLRIQMTFQGVEFRSIVGQTDVSAATG
                     KTATTNDGGATSKKNTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTSSKSVSV
                     KNGESTFSDAANEQASKVLKPGNALRKYSVNPNKKGTY"
     gene            complement(7079..>7477)
                     /locus_tag="ACHUT9_003824"
     CDS             complement(7079..>7477)
                     /locus_tag="ACHUT9_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HFR1350199.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
BASE COUNT         1979 a         1679 c         1728 g         2091 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag ctcgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaatggtat gctctttact cgctttaaac
     6541 tgagtaatga tactatcagt gatagctatt ccatctctac aaacaatagt gatgatttgc
     6601 ttctggtctc gccattgttc aaccgtagaa ataaaggttt ctacaagttg cccctgtctg
     6661 gttaatagaa ctgaacccgt cgaatcaacc actacaacac cgctaataga gatggtctta
     6721 ggtgctctct gaacattatc agtgatagtt tgccccgatt gcatagcctg agttgtcacc
     6781 atcatcggtg agtcatagtc catctgttca gtagctgtta aggcaagaaa agattccaca
     6841 ttatctctaa ggtgaaaata gataccatct ttacctgagt atctaatttg ctttgtgtca
     6901 aacattgttg ttattcctat tctgttaagc gtgagctacg tgaaatctaa tttttgatgt
     6961 tggtatctgt tcagaagatt tcacgtagca ttcgactatt catagttgaa tcgtcatact
     7021 agataaagat taatagattg ccatgtgtgg caataaatat tagtagctct aacattgttt
     7081 attcctctgg ataagctgag ctaatcaagg tatcttcctg catacgatta atatgcgatg
     7141 tggttctttc atcaataacc tttccatcaa gtaccgtctg agttgtgata ttgattgtct
     7201 gaggctgaat atttactgac ggtttgataa cctttaaatc ttgtgtgaat gtttccactc
     7261 gacctttcag attatttaat gcttcaggtg aaaactgtgg aacatctttt gatttgccct
     7321 gaaccatagc ccataaaaca gcggctaaag agttcatgtc accagtcaat gcttggtctg
     7381 tgggttccac ctttgacctt acaactttat caattggatt atctgactta ctcatctgac
     7441 gttcatagaa cggtttgaca atggcattaa tcacatc
//
LOCUS       DAZPEP010000188         7358 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZPEP010000188 DAZPEP010000000
VERSION     DAZPEP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7358)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7358)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7358
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.189"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..959)
                     /locus_tag="ACHUT9_003825"
     CDS             complement(<1..959)
                     /locus_tag="ACHUT9_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1350200.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGPQI
                     YFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEE"
     gene            complement(1560..1808)
                     /locus_tag="ACHUT9_003826"
     CDS             complement(1560..1808)
                     /locus_tag="ACHUT9_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFR1350201.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(2071..2166)
                     /locus_tag="ACHUT9_003827"
                     /pseudo
     CDS             complement(2071..2166)
                     /locus_tag="ACHUT9_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2163..2729)
                     /locus_tag="ACHUT9_003828"
     CDS             complement(2163..2729)
                     /locus_tag="ACHUT9_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFR1350202.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            3039..4811
                     /gene="aspS"
                     /locus_tag="ACHUT9_003829"
     CDS             3039..4811
                     /gene="aspS"
                     /locus_tag="ACHUT9_003829"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFR1350203.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            4929..5381
                     /gene="nudB"
                     /locus_tag="ACHUT9_003830"
     CDS             4929..5381
                     /gene="nudB"
                     /locus_tag="ACHUT9_003830"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFR1350204.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            5410..6150
                     /locus_tag="ACHUT9_003831"
     CDS             5410..6150
                     /locus_tag="ACHUT9_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1350205.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHUT9_003832"
     CDS             6185..6706
                     /gene="ruvC"
                     /locus_tag="ACHUT9_003832"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFR1350206.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6708..7310)
                     /locus_tag="ACHUT9_003833"
     CDS             complement(6708..7310)
                     /locus_tag="ACHUT9_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFR1350207.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1873 a         1646 c         1931 g         1908 t
ORIGIN      
        1 tgctcttcat gttcaaaagc atgccatatc tgtgatacat cagattgttt gttttccggg
       61 aaccatgctg tcacggcatc agccaggggg cgatggagtg tattctgttg tccgtcactc
      121 atggagaagt aaatctgcgg gccgtggtag tccggcgaag aggttaatct ttgcagggat
      181 tgcagagcat gggatgacaa tgggttatca ctaatatcta ttgaacattc attcctcaga
      241 ttaagaatgc tttccgggat atgacttatc tgatttatgt caaaataacc gttccttaaa
      301 ttttccggca ggtatggaat atcggttaac atattgtcga ttgcccatat ggagtgaatg
      361 ttgtgaggta gaggggggag ttcactaaga agattcccta ttgcgctaat ctcttgtaaa
      421 gaaaatggta aaggtggtaa aactgccagc ccattacatg atacatctaa tgtttccagt
      481 agttctggta aaacaggaag agaacataat tgattatttg agacatgaag ctccttcaaa
      541 gatatgggga gtgtgggtag tgtgattagt tgattgtggg acgcattcaa taatttaagt
      601 ccttgaggca aagcaggcag ttcaataagt ctgttatagc tgacattaag ctgtgtaagg
      661 gacgcaggca atggggagat taagcttaaa ttatttttac ttatattaat tgatttaatt
      721 cccgggggga tttcaggtaa tgttgtcagg cctaattcag acaagtccag gttcgtctct
      781 tggttttgta gacatgatac tagtcgctga aaagcgatgt ctcgttgttc ttcttgtatg
      841 cggttatttt tccattcagt ccaatgggtt agataatttt catatgcacg gctagtgtcg
      901 attgaataag tggagaacga atttgaaatt aatctgtgat tgttatttgt cgggagcata
      961 aatatcaggg cgtattcttt atgttgagag gtgttggatt cttttttatc tgctttgcaa
     1021 tgttattggt tcccttcagc agtagacagg aacttctgga ggaggcggat atatagtttg
     1081 tgtaagcata ttaactccat atgttatata ttgaagaact tctgctttac actatcctaa
     1141 actggatcgg tttaatgtaa aaaaacgata ctttttgggg gagggggcaa tttttcctta
     1201 aggtatgaaa aaaggagctg aagctatata atagctatta atgccactgg tatcagtgaa
     1261 tcatgtatat aaaaaactgt gttaaggagg ctgtccatta gcggtttttt attcttctgg
     1321 aaccgcggca tctttgcgta tctttttgtc ttctcaccgt ctggtcggtg ttttgctgag
     1381 actgctaatt tcctgtcttt gctagtgctg tactggtact gcccaatcat gattggcggg
     1441 ggcgggcgga gttgagaccg cagacacgtc gtatgcaaga acgtgctgcg gctggctggt
     1501 gaacttccga tagtgcgagt attgaatgat ttccagccgt tactgatttt acgtgctaat
     1561 tagtgaacaa accactcgtc agcagactcc caggtatctt tcagagtttc ctgaacaaaa
     1621 gttttagctg aatctttatc ggcggtgcgc gtaacagaaa ggccatcgtt gctggtggct
     1681 tttacgatca cctctacatc gtcataacgt ttactgatgc gtcgggttaa ttcttccttt
     1741 aacgcatcca cagcaccggt tggcatttta gtcattttct ctttggctat gcagatttca
     1801 atacgcataa aaatccctct acactgtgtt tttatacagt attattttta actgtatgga
     1861 taaacagtgt caagagtgag ggaaagtgat tttttggggc atgcatgggg caaaaatatt
     1921 tgttgtgggg cattttgggg catgattggg acactttgtt acatatgaac tttgccgatt
     1981 ttcatatggc atggcaatcc aacgctttga ttattctaaa gaaaacacat gctcttgggc
     2041 gttctttagt gatttttaaa aatcccgtat cacgcagtta aagtggcggg catactcttc
     2101 aaggctggtg atccccaacc gcacccattt aggatgcgac cattgcggta gaccgatgta
     2161 aatcataacg cgtgaaggat ctcttccacg ctacgcacac gggcgatgcg cgggtagata
     2221 tgattaatgc tgttattgtg ttgctcggcg ctggcggcac tacaggcgtc ttcggcaatc
     2281 accagattaa agccaagttc ccaggcattg cgggcggtgg attcaacacc gatattggtc
     2341 gagatcccac ataacactat tgtatcgata cctcggcggc gcagttgcaa ctccagatcc
     2401 gtaccgtaaa acgcacccca ctgacgtttg atgatttcga tatcactgtc agttgcacct
     2461 aatgcagctg gatgttgcca ccagttttcg ggcaacactt tagcggggga gggggcatca
     2521 accggctgtt ttaatgcttc ggcgtaatcg gcagaccagc caactcgcac cagaaacacg
     2581 ggctgaccgc tggcgcgaaa tttcgccgcc agcttcccgg cgcgattaac tacctcatcg
     2641 gcagtatgtg gaccaccggc aaaaggtaag atgccttctt gtaaatcgat caccaccaac
     2701 gcagtggttt tagcattaag ttcaagcatg ctgactcctg aaaaaacaaa atcgtgcctc
     2761 acaccttaat gcgattagcc gatggcggaa cgacaaattt tgttaatttt tatgagttcg
     2821 ggcaacacct ttatagcaaa ctggcgtaga gtctgatttc aggcccgata taaagtggta
     2881 acgaggcgtt acagcgcggg ccaggctctt acggtgcggc agaatttcca gtataatagc
     2941 cgcctttttt cattcagttg tgacatacag ctaacgctgc gacttggtca cctgcgcagc
     3001 agggggcatc cgccggcagc taagttaagg gatatctcat gcgtacagaa tattgtggac
     3061 agctccgttt gtcccacgtg gggcagcagg tgactctgtg tggttgggtc aaccgtcgtc
     3121 gtgatcttgg tagcctgatc ttcatcgata tgcgcgaccg cgaaggtatc gtgcaggtat
     3181 ttttcgatcc ggatcgtgcg gacgcgttaa agctggcctc tgaactgcgt aatgagttct
     3241 gcattcaggt tacgggcacc gtacgtgcgc gtgacgaaaa aaatattaac cgcgatatgg
     3301 cgaccggcga aattgaagtg ctggcgtcct cgctgactat catcaaccgc gcagatgttc
     3361 tgccgcttga ctctaaccac gtcaacaccg aagaagcgcg tctgaaatac cgctacctcg
     3421 acctgcgtcg cccggaaatg gcgcagcgcc tgaaaacccg cgctaaaatc accagcctgg
     3481 tgcgccgttt tatggatgac cacggcttcc tcgacatcga aactccgatg ctgaccaaag
     3541 ccacgccgga aggcgcgcgt gactacttgg tgccttctcg tgtgcacaaa ggtaaattct
     3601 acgcactgcc gcaatcccca cagttgttca aacagctgct gatgatgtcc ggttttgacc
     3661 gttactatca gatcgttaaa tgcttccgtg acgaagacct gcgcgctgat cgtcagcctg
     3721 aatttactca gatcgatgtg gaaacttctt tcatgaccgc gccgcaagtg cgtgaagtga
     3781 tggaagcgct ggtgcgtcat ttgtggctgg aagtgaaggg cgtggatctg ggcgatttcc
     3841 cggtaatgac ctttgcggaa gcagaacgcc gttatggttc tgataaaccg gatctgcgta
     3901 acccgatgga actgaccgac gttgccgatc tgctgaaatc tgttgagttt gcagtatttg
     3961 cggggccggc gaacgatccg aaaggccgtg ttgccgctct acgtgttccg ggtggcgcat
     4021 cgctgacccg taagcagatc gacgaatacg gtaacttcgt taaaatctac ggcgcgaaag
     4081 gtctggctta catcaaagtt aacgaacgcg cgaaaggtct ggaaggtatc aacagcccgg
     4141 tagcgaagtt ccttaatgca gaaatcatcg aagccatcct ggatcgtact gccgcgcaag
     4201 atggcgatat gattttcttc ggtgccgaca acaagaaaat tgttgccgac gcgatgggcg
     4261 cactgcgcct gaaagtgggt aaagaccttg gtctgaccga cgaaagcaaa tgggcaccgc
     4321 tgtgggttat cgacttcccg atgtttgaag acgacggtga aggcggcctg acggcaatgc
     4381 accatccgtt cacctcaccg aaagacatga ctgctgcaga actgaaagct gcaccggaaa
     4441 atgcggtggc gaacgcttac gatatggtca tcaatggtta cgaagtgggc ggtggttcag
     4501 tacgtatcca taatggtgat atgcagcaga cggtgtttgg tattctgggt atcaacgaag
     4561 aggaacagcg cgagaaattc ggcttcctgc tcgacgccct gaaatacggt actccgccgc
     4621 acgcaggtct ggcattcggt cttgaccgtc tgaccatgct gctgactggc accgacaata
     4681 tccgtgacgt tatcgccttc ccgaaaacca cggcggcagc gtgtctgatg actgaagcac
     4741 cgagctttgc taacccgact gtactggctg agctgagcat tcaggttgtg aagaaggctg
     4801 agaataactg atatgactca aatacacgaa atcattcgcg ttgcatcaag gcggcaactg
     4861 agtgaactcc catgagcata gataactatg tgaatgggat gagcgaaggc agtcaacgaa
     4921 gaggcagcgt gaaggataaa gtgtataagc gtcccgtttc gatcttagtg gtcatctatg
     4981 cacaagatac gaaacgggtg ctgatgttgc agcggcgtga cgatcccgat ttctggcagt
     5041 cggtaaccgg cagcgtggaa gagggtgaaa ccgcgccgca agctgccatg cgcgaagtaa
     5101 aggaagaggt caccattgat gttgtcgctg aacaactgac cttaattgac tgtcagcgca
     5161 cggtagagtt tgaaattttt tcacatttac gtcatcgcta tgcgccgggc gtgacgcgta
     5221 atacggaatc atggttctgt cttgcgctcc cgcacgaacg ccaggtagtc ttcactgaac
     5281 atctggctta caagtggctt gatgcgcctg ctgccgcggc gctcactaag tcctggagca
     5341 accggcaggc gattgaacag tttgtaatta acgctgcctg aacaggcagg gctatctgga
     5401 gatattttta tggcaggtca tagtaaatgg gccaacacca gacatcgtaa agctgcgcag
     5461 gatgctaagc gcggtaaaat cttcactaaa atcattcgtg agctggtaac cgcggctaag
     5521 ctgggcggtg gcgatccgga cgctaacccg cgtctgcgtg cggcggtgga taaagcactg
     5581 tctaacaaca tgacccgtga cacactgaac cgcgcaattg cacgtggtgt gggcggtgat
     5641 gatgatgcaa acatggaaac catcatctac gaaggttacg gtcctggcgg cacggcaatc
     5701 atgattgaat gtctgtctga caaccgcaac cgtaccgttg ctgaagtgcg tcatgcattt
     5761 agcaaatgtg gcggtaacct cggtactgat ggttccgttg cctatctgtt cagcaaaaaa
     5821 ggcgtgatct ccttcgagaa aggcgatgaa gacaccatta tggaagcagc actggaagca
     5881 ggtgctgaag acgttgtgac ctatgatgac ggcgcgattg acgtctacac tgcatgggaa
     5941 gaaatgggta aagtgcgcga cgctctggaa gcggcaggtc tgaaagcaga cagcgcggaa
     6001 gtttccatga tcccgtccac caaagctgat atggatgcag aaaccgcacc gaaactgatg
     6061 cgtctgatcg atatgctgga agattgcgac gacgtgcagg aagtctacca taacggtgaa
     6121 atctctgatg aggttgcagc aactctctga tggggcctgc taaacagcaa aacggagacg
     6181 cgtgatggct attattctcg gcattgaccc gggttcgcgc gtgatcggct atggtgtcat
     6241 ccgccaggta ggcaggcaac tgtcctacct gggtagcgga tgcatacgca ccaaagtgga
     6301 tgatttaccg tctcgtctga agctcatcta tgcgggcgtg acggaaatca tcacccagtt
     6361 ccagccagat tatttcgcca ttgaacaagt ctttatggcc aaaaatgccg actcggctct
     6421 gaaactgggg caggcgcgcg gcgtggcgat tgtggcggcg gtgaatcagg aattgccggt
     6481 atttgaatac gcggcacgtc aggtaaagca aacggtggta ggtattggta gtgccgaaaa
     6541 aagccaggtg cagcatatgg tccgcacctt gctgaaactg cccgctaatc cacaggcgga
     6601 tgccgccgat gcgttagcta tcgcgattac ccattgtcac gtcagccaga atgcaatgca
     6661 gatgagcgaa tcgcggctga acctggcgag agggcgactg cgttaagtta taccgcctcg
     6721 gtcagttcag gctgaggcgt ttcaactccc tccgcgtgaa tcaacaccag acccggatga
     6781 tgccacaaac tggctggcgt gacggtttta cgctcccacg gaatagaacc taagaaccag
     6841 aatttccaga aagtgagttt tgcattcgga ttgctattta acagttctcc aaacgtttct
     6901 atttcaccca ccggaatgca tagcgcttct ttataaatat caaaaacaaa ttttgaacag
     6961 aactggcgcg aagattcgta tttaaaaccg gtgtggtaaa gtttgcgtag ccgggaggga
     7021 acctgttcaa caattcgttg tttttgttgt tctgtcagcc cagcgtctaa tcgctttata
     7081 gcatagcgtt gattagacga acgtttaata aaacgagata gcgtggtgat ggttgaaagg
     7141 ggaacccggc tttctgcaac caggaagtct tcaccgttat gaccgataat gatcccgacg
     7201 tgattactcc agcaatttga tgcagctgat atttgaccaa ataaggcagc acctatacat
     7261 gtaaagacga tatctccaat ttcatattca gcagggtaat taatattcac gttgtcattc
     7321 cattgaaata gatacgaata tacgtaatag cataaaaa
//
LOCUS       DAZPEP010000189         7273 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZPEP010000189 DAZPEP010000000
VERSION     DAZPEP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.190"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(1..4519)
                     /locus_tag="ACHUT9_003834"
                     /pseudo
     CDS             complement(1..4519)
                     /locus_tag="ACHUT9_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616597.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            complement(4539..5000)
                     /locus_tag="ACHUT9_003835"
     CDS             complement(4539..5000)
                     /locus_tag="ACHUT9_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFR1350208.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5028..6930)
                     /locus_tag="ACHUT9_003836"
                     /pseudo
     CDS             complement(5028..6930)
                     /locus_tag="ACHUT9_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT         1380 a         2208 c         1962 g         1723 t
ORIGIN      
        1 tttatttgac tacgtgataa acgaacccca tccaggaaac ccgtatgccc actaacacga
       61 cgaccatcgc caatatagtc catcaaactg ctgattttag aacttgcagc caatcccagc
      121 ggatcaaccc acccacaagg attcggcaca taccggtaag tctgctcccc gccctgcaac
      181 cccagcggat cactcaccag gtacattccg gtttccggct cgtaataccg gaaccgatta
      241 tagcacagcc cggattccgc atcctgccac tgtcctgcat acagcaggcc ggggtcggct
      301 tccgggtcca gttccccgcg cgggtccggg tagccggtgt ctgcgggcag gctgagtgcc
      361 agcccccaca ggctggtctg ccccggtcgc cagaccgtct ttccttcact gttaaagagc
      421 atcagcgggc ggcccgtcag gtcgctcacc gcatgattcg tccgcgtcac ccactgcggg
      481 gccaccgacg gatgcgcctg ccgtacccgg ctgaactgct ggcttatcag ctcaaagccg
      541 ttaaacacca ggtgccttac gctgtacagt ttatcatcgc ggtattcccg gatttccgca
      601 atactgtcgc cgtcccacag atacgtgaag cggattttct tccggtcgca gcgtttctct
      661 gttcgtctgc cgctggcgtc gtggcggtat tcccacagct caccctgtgc gctgcaatac
      721 ccggtcagct ggtcccggct gtcccaccgg aaccgctctg tttccggtcg gtagccgtca
      781 cggtgtctgg tgcggctgac catccggcct gcggcgtcat agtcatactg cgtgtttccg
      841 gcctgtttca gccggtggcc tccggcatac tggtcagtct cttcacttat ccggtgcctg
      901 cccgcagact gcgccttcag gtagccgcag ctgtcatagc cataggactc atcgccttcg
      961 ccatagccca gcccggtgcc tgaggctgtc accgccgtga cctggccgtt ccggtccagc
     1021 cgatactccc gccgtgtttc ccgtccgtaa tcttcccggc tggtgacgcc tgacacgtta
     1081 cccgcgctgt cgtaacggta ctcccgtacc agtgagggaa ccagcccgtc ccatgtggct
     1141 tctgccggaa aaaatccggc gcgctgactg acaggctgac cggctgcatt ccagccctgt
     1201 tcacaggcaa aaccccggtt actgtgccgg agcacttcac gtcccgcctc atcacggctg
     1261 aacgtcagcg tctcatcccc cggcagaatg acctctgtca gttctccggc ggcgttgtag
     1321 cggtacgtgg ttttcagcag ggattcaccg gggtgtctgt cgcgttcatc ttccggtggc
     1381 aggatacagc ggatgacggt gtgttcatcg gggtagttgt attcaatggc ccgtcggccc
     1441 tgagtggtga cattcacccg cccgcgttcg tcatagccgg tgcgctgtga tgtgccgtcc
     1501 gggctgaaga tacccgtcag cctgccggat ttgtcatagg catactgcgt tttgccttcc
     1561 ggcgagatga tttctgccag gcggcctgcg gcgtcccggc tgtagaggat ggtttcctcc
     1621 aggccgttaa ccctggcggt acagaggcca tcggcgtcat agcggtaacg ccagaccacg
     1681 ccgccccagt cacgctcttc cgtcacccgg ccgcagttat ccagaacata aagatatgac
     1741 tcaccctgcg ggttgatgac ttctgtcagt tgcagcgtgt ctttatcgta gcggtactgc
     1801 cagcggtggc cttcaccgtc cgttcgcgcc accggaaaat cgaacggacc gtattccatc
     1861 gtccagacaa caccgttgcc gtccgtccag gccagcaggt tgccgtggcg gtcatagcga
     1921 aatccttccg cgccggttcc agcacgttca atgccgctca gtttatggtg ctgctgccac
     1981 tgtaaccgca ctgactgacc gccggggccg cgcatcttca ctggcaggtc gtgaccgctg
     2041 tattcggtgt ggtgttcacg gcccatcacg tcggtgagtg tttccggccg gttgtggtgg
     2101 tcgtactcta tgcccagacg ccgttcatcc tggcggaata ttcccgacag cagcccctga
     2161 gcggtgtatg ccagccgggt ctcttcgccc tgtggcgaca ccagacttac cggcaggccg
     2221 cgttcatcac actgctgtgt ccacgtctgc ccggcagggt cggtgatgtt gctgagcagg
     2281 ccgcgctcat catagcggta ctgccactcc gcgccgtctg ccgccgtgta acggcttaac
     2341 tgaccgtgtg cggcgttgta ttcaaatttc tccacccgcc cggcagggga gtggcggggc
     2401 agcaggtgat ggaattcatc ccattcatac atggtggtgc tgccgtccgg cgcttcttcc
     2461 cgcagaatat tgccgtcagg atcatagtaa taacggtgaa tgcccttgcc gtccgtcacg
     2521 gtggtgcagc cgtcaccata gtccagcgtg gcgttgtaat agccctccgc cccggtgaca
     2581 ttcacgcacc ggccctgttc atcgtaggtg aaacggctcc acgtctggtc gttatcggac
     2641 cagcggatga tccggtccgc agcgtcgtac tcataaaaca ggtggtagtc cagccgcgca
     2701 tccgcttctg tcagccgccc ccgcgcatcc tgttcgtagg tcgccagcgt ctgtatgtcg
     2761 ttatccgtgc ggtgaatggc tttcaggtag ccgctggcgt ggtacgtcag tttaagccgg
     2821 atcccgtcgg tgtgactgac acccgtgatc cggtactggc tgtcgcgctc aaacgtgatg
     2881 cggttgctgg cggtgtcgct catcccgccc agcatgtaca tccggtcatt ggggctgtgt
     2941 gccaggcggt catcgctggt atcgtcagtc accgggtggc gcagacgcat tcccggaaag
     3001 gcgtcataaa agaagcggct gctcagtgca tccctgtcga acagctccag ataatcatcg
     3061 cggcggcgca gaatgtactg ggcgtgatac gggttaaccg ccgtatcact gtcaccgtcg
     3121 aaggcaaatc tcagcgtggc tccctggctg atgatatcca cccggtttcc ctgttcacgc
     3181 acccaggcgt attcgctcca ggagtcgctc cagcccacgc cggtcaggcc atgcagcggc
     3241 agtacagaac tgtggaaacg ggtgaaggag agggggaggg tctggcccag ggtgaagtcg
     3301 gtacgttcgt cgcagtatgc gccggttacc ggatcgacag gatctgttac ccatctgatg
     3361 actttattgg caatactttt taacgactta cctgttttcc ccagcaacgc gccgagcttt
     3421 gcgcccagca gcttcccgct tttccccagc agtttcccag tttgccgagt ctcgccagat
     3481 tcttcagttt tcccagtagt cccgctggcg gcagaagata gctggctatc gttattcccc
     3541 ccaggatcat tctctgccac ggcgggaact ccggctggat atccagatat gtgacctgct
     3601 caccgccaat aaagacgtcc ggcgaacctc ccacgatcgc cgcactgcac tccagtttgt
     3661 cgcccttgcg cgcggcaggc tgattgttga taaagacgtt gctgctgccc tgggctatcc
     3721 tcggtggttt gttctccttg tcgcagagcg ccgtgtcctc accggcttcc gggaaatcca
     3781 catctttttt gatttcgcca cgcccgccca ccatggtggc aaactccatt aataccggac
     3841 cggcgggaaa cagaagcgat gcgccggcgc tgacgcgatc cataaaaccg gcattgctgt
     3901 ccgtcagggt gataacagcg ttcccccagg ccttcgccag tggtactgct ttttccgctg
     3961 ccgattcaag caggtcagtg gcaatatcac cccatgaggg ttccccctgt gggacttcag
     4021 gtattgctcc aggtggggga ggagggggaa gggtgaccgc tgcacgtgcg gccagctcgc
     4081 cgttaatttt tacgttttca gaaccggtat tgatggcgcc gtccgtgtca caaaagctgt
     4141 ttatccatga ttcaagctgg ttctgtttct cctccacata atcactcgcc ttatagctgg
     4201 catacatcag cgcaggtacc agaaccagcg cataagggcc ggtggcacag gcggccataa
     4261 tcaggcaacc ttctgcgatg gtcactatcg cccccagtac ggcccctgcc agcgccccca
     4321 aaaaactctt gtgctgaatt tcatcgccca gcctggcggc gggggggcca gacttttttg
     4381 atgccaacat ctgtgcaatg gctttgtcgt tggctgcccg tttcatctgg ctggcgacca
     4441 tcgcctgctg tgacagcatt cgcattgcca gcgtaccgcc tgcggtcgct ccctgtggcc
     4501 cgcctggtcc ttcactcatg tgttatccct cgtttatgtc agcgcggctg gtccttttgg
     4561 atgtcattct ccggtggcaa cggggtgaag ctctttatca cctccagcag catcgtcttt
     4621 tgactgttct tcagggataa ggaggtgctg aaagtgaagg tcagcagcgt atctccacgc
     4681 cgctggaata cggctatctg gctaatccgc ccctgattcc cctcatcttc cggaccgttc
     4741 caggaataat ccagcagcca ggccggggcc tctgccagag tgatttcccg gcaggcatgt
     4801 tcggtgtacc catcaagttt gccctggtac agcgccagcg tttcctggta cagctctggt
     4861 gtcagggttc cctgtggaac ggcttcgcgg gtaatgacca ggttgatgcc actgttatct
     4921 gtggacacca ggacctgcat tgactgatcc tgccattgag acggcagttg aatgtggcct
     4981 tcattaaaag tgaactgcat aaaatactcc ctgataatct gagtgaatca gttgatgaaa
     5041 acagaaccgc ccacgatggt gatgccatcc ttcgacagcc tgattaccgt cgattccccg
     5101 acctgtagct gaatgctatc cggcgtcagc gcaattttgc tttcctccac ggccagcaaa
     5161 atttccttaa tcgccgtata agcctgtata ccgtcgcctt cgccaccatt gtttacagcg
     5221 tcaacatgca ccgaaccggc aatcgtgcct ctgtcctgaa agacatcctc agataaagac
     5281 cctttcagta ctccggtgat cttgtggcct ttttccaccg taagaaggcg gttaccttcc
     5341 tggatcgtga ccgtctggtc attttttacg gtttcgcggt gatcgtgttt tacggtggtg
     5401 gtacggtcgt tgagcacttc ggtatccatg ttcttctgcg cgtggatgta gacctgttct
     5461 ctccctgtcg catcatcaaa cttcagctca ttaaatccgc tgcccatgta cgttttggag
     5521 cggatggtca tctgcgtctt tgttcccggc aggctgccgg ggggggcggt tttcctggtg
     5581 gtaggtgcgc cccataatga tcggctggtc cggatcgccg ttgaggaagt ctacaatcac
     5641 ttcctgaccc acacgcggta tcgccaggtg gccaaaaccg gtgcctgccc acgcctgtgc
     5701 cacacggatc cagcacgaac tgtcttggtc tgccgggtta taacggtccc agttgaactt
     5761 cacccgcacg cgaccatgtt cgtcgcagaa gatttcctcg cctgccggtc ctgtcacgac
     5821 agcgctctgc gggccgtcga ccagcggttt cagcaacggc tgtggtcgcc atgttctgtc
     5881 tgccgggatc accgcaaaat ggttctccag cgccgtcccc gctccctgcc gtcctggcac
     5941 cgcctgtggc tgttcgccgt gcagttcact tgccaccacc tgccattccc ggttcaggtt
     6001 cgcctgcgga tgccccgtca gcacaattcg tcgtcccggc catatctccg gcgagcgact
     6061 cattccccgc gcggtttctg cattgtttcg ccagccgtcc atctgccagc gggcaaagtt
     6121 ctgcccgtga gcacccttga aacgtccggg gtagtcatac acttcatact gcgtgcgctg
     6181 gtagtcctgg tgctggcctt cctgttcaaa acgtccggcc cagccggggc gtttaaaggt
     6241 gtagtctttg gtcaccacgg aagaagggcg gatttgtgcg ctgtagcgga actggctgat
     6301 gcagagggtg ctcacctcgg tacgggtgtt cgggttccat gggatttcaa aagattcggg
     6361 cagatggcgg actgtgtcgc acagcaccag gctctggtcg gtacttttgt aagcatgctc
     6421 ctcataaaag aagatgcctt cctccgccgc catccggcac aggaaatcgt aatcagtctc
     6481 accgtactgg acacaaaact cacgggaagg atgcggctcg ctgaacagcg gactccattc
     6541 ggtcaccccg ttttcctgca acatcgtgcc gaggatgctt ttgatgtcct cgttctggaa
     6601 gatacggaag ttctggcgca gaccggcacg ccacagcggc gggtgcacct tcatgctgta
     6661 cagcatctgg tttttgtcgt tctccccgag ttcaaaccag gtcaccacgc ctttcacccg
     6721 gcgctgtact tcatcgccct gccatatcgt caggtaggcc attttgtcca gcacctgcgc
     6781 aaattcaagg gagagaaact gctggctgac cagggagaga tcgagggaaa agagcgaaga
     6841 gagtgactgg ttcagatgaa aggaaaccac cgcaaaagcg tccggcggca ggccgtccac
     6901 ttccagtgtg aaacgtaatc cggttgacat agcaacctcc agtaaaatga ataatgatcc
     6961 gtaccgtaca actgcggata atgtgataag cgggacgact tcagacgtta ttagggaagg
     7021 aacagaaaat gctatggtta tgtgagaatt ttgtttaaat attactttac agaaacgggt
     7081 gtctgttcag tcaaaaaaat aatcacaagc tcaacctctt gtttcttggc gttattcagg
     7141 acaggtacgc atatcacaac gagtaacaaa aacgcaaagt taaaccaata ttcataatat
     7201 atttttattc acagcaataa ataatccaat taacattatt aaaatgaatg attgcataag
     7261 aaatatatgc tac
//
LOCUS       DAZPEP010000190         7122 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZPEP010000190 DAZPEP010000000
VERSION     DAZPEP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.191"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..436
                     /locus_tag="ACHUT9_003837"
     CDS             <1..436
                     /locus_tag="ACHUT9_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFR1350209.1"
                     /translation="KVALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQF
                     AVQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAK
                     AKLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            519..1421
                     /locus_tag="ACHUT9_003838"
     CDS             519..1421
                     /locus_tag="ACHUT9_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFR1350210.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHUT9_003839"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1476..3638)
                     /gene="uvrD"
                     /locus_tag="ACHUT9_003839"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFR1350211.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHUT9_003840"
     CDS             complement(3722..4438)
                     /gene="yigB"
                     /locus_tag="ACHUT9_003840"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFR1350212.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHUT9_003841"
     CDS             complement(4438..5334)
                     /gene="xerC"
                     /locus_tag="ACHUT9_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFR1350213.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENV
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5331..6038)
                     /locus_tag="ACHUT9_003842"
     CDS             complement(5331..6038)
                     /locus_tag="ACHUT9_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFR1350214.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHUT9_003843"
     CDS             complement(6035..6859)
                     /gene="dapF"
                     /locus_tag="ACHUT9_003843"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFR1350215.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6896..7099)
                     /locus_tag="ACHUT9_003844"
     CDS             complement(6896..7099)
                     /locus_tag="ACHUT9_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFR1350216.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1639 a         1989 c         1856 g         1638 t
ORIGIN      
        1 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
       61 gttttatgtc gcgggagata aagtcgacat cgttcgtctg gatgccttca aaaccagtaa
      121 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacagtg taattaatcc
      181 gcgtattcat cccaacgtcg cctcacgtaa aattcgtaac ggtgttggga ttaataaaca
      241 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
      301 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
      361 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
      421 cgagaggaaa ggttaagcat tgtacactcg aggaggccag acaaactggg gctggctaca
      481 cagctgaaaa atcatcagat aaatgaaggg aaacaaaaat gaaaaaaacg ctcgcaacgt
      541 tatttttatt aacgtgtctg ggatcatcat ccgcttatgc ggataacgct ttgattttac
      601 aaactgattt tagcctgaaa gatggtgccg tctcggcgat gaaaggggtc gcattcggtg
      661 ttgaccataa tcttaaaata tttgatttaa cacacgaaat tccaccgtat aacatctggg
      721 aaggcgctta ccgcctgtat cagaccgcca gttactggcc gcaaggttcg gtatttgtca
      781 gtgtggtcga tcctggtgtt ggtacagacc gtaaatcggt agtgctaaaa accaaaaacg
      841 gtcaatactt cgtttcacca gataacggta cgctcacttt ggtggcggaa tcgctgggta
      901 ttgagagcgt ccggcaaatc gatgaaaaaa ccaaccgcct gaaaggttcc gaaaagtcat
      961 ataccttcca cggtcgtgac gtgtatgcct acaccggtgc acgcctggct tccggagcga
     1021 tcacgttcga acaggtgggg ccagagcttc ctgcaaaagt tgtcgaactt tcttatcaga
     1081 aagccaaagc aacaaaaggt gaagtgaagg gcaatatccc gattctcgat attcagtacg
     1141 gcaacgtctg gagcaatatc agcgacgagt tgctgaatca ggcaggaatc aaactcaacg
     1201 acacgttgtg tgtaaccatt tccgaaggtt cccagcaaaa atacgcaggg aaaatgccgt
     1261 atgtcgcaag cttcggcgat gtgccggaag gccagccgat ggtgtatcta aacagcttat
     1321 tgaatgtttc cgtggcgctc aatatggata atttcgcgca gaaacatcaa gttgcgtctg
     1381 gtgctgactg gaatattgat gtgaagaagt gcgctaagta aggtgctgat gcaggatgcg
     1441 gtgtggcgca ccgcatcctg caacggagtt gtaaattaca ccgtctccag ccgggcgtaa
     1501 gccgccacca gccatttgat tccctggccc tggaatgcca cctgcaaacg gctatgttca
     1561 ccgctgcctt ccatattgac gatggtgcct tcaccaaact tagcgtggcg cacgcgctgg
     1621 ccgagtttgt agccgctgtc gttctcgacc atcggcgtac ccatccgctg atggctaacc
     1681 gggcggctta ccgtggcgcg caggcgcacc tcttccacac actcttccgg cagctcgccg
     1741 ataaagcgcg acgggcgatg gtaaacctct ttgccataca gacggcgagt ttccgcgtag
     1801 gtcagcgtca gtttctgcat cgcacgggtt acgccaacgt aggccagacg gcgttcttct
     1861 tccagacgtc cgccttcatc cagcgacatc tggcttggga acatgccctc ttccataccg
     1921 acgataaaca cctgcgggaa ctccagccct ttcgccgagt gtagcgtcat caactgcacc
     1981 gcatcctgcc aagtatccgc ctgcccttca cctgcttcca gtgccgcatg ggagaggaac
     2041 gcctgcagcg gcattaaatc ttcgtcttct tcgttgtagc tgaactggcg cgttgccgtc
     2101 accagttcct ctaagttttc gatacgcgtc tgaccttttt cgcccttctc ctgctcgtac
     2161 atggtacgca ggccggagtc tttaattacc cggtcagtct gtacatgcag cggcatatcg
     2221 gcagtttctt gcgctaaggc atcgattaat tccataaatc gctgcaaggc gctggcagct
     2281 cgcccggcga gggctttttc ctgcaacagt tcacgacatg cctgccagag tgttaactgg
     2341 cgatcgcgcg atgtctgacg taccacgtcc agcgtccggt caccaatacc ccgcgttggc
     2401 gtattcacca cgcgctcaaa cgccgcgtcg tcgttacggt tggcaatcag acgcagatac
     2461 gagagcgcgt ctttgatttc ctggcgttcg aagaagcgca tcccgccgta aatacggtac
     2521 ggcatactgg cctgcaacag tgcttcttcc agtacacgcg actgggcgtt actgcggtag
     2581 agaatggcgc actcggcaag cgccccgccg ttgtcctgcc aggttttgat gcggttaacc
     2641 acaaaacgcg cttcgtcgag ttcgttaaaa gcgcaataga gggaaatagg ctcaccgtcc
     2701 gcgccatcgg tccacagttt tttacccaga cgcccgttgt tgttttcaat cagggcgttc
     2761 gcggcgctca gaatattgct ggtagagcgg tagttctgct ccagacggat agtttcagca
     2821 ccggggaaat cattgaggaa acgctgaata ttctctacct gcgccccgcg ccagccgtag
     2881 attgactggt cgtcatcgcc aacgatcatc actttgccgg tatcgcccgc cagcaggcgg
     2941 atccacgcgt actgaatgtt gttggtatcc tggaattcgt ccaccaggat gttggtaaaa
     3001 cgttcgcggt agtgttgcag gatatgcggc ttgttaagcc acaactcgtg agcgcgcagc
     3061 agcagctcgg cgaagtccac caggcccgca cgatcgcacg cttcctgata cgcctgatac
     3121 accttctgcc aggtctgctc caccggatta ccgtagcttt gaatatgatg cggacgcagg
     3181 ccttcatctt tctggctgtt gatgtaccac attgcctggc gcggcggcca ctgcttctcg
     3241 tcgaggttca tggctttgat cagacgctta agcaggcgta gctggtcttc actgtcgagg
     3301 atctggaaat cctgcggtag attggcgtcc atatggtgcg cacgcagcaa acggtgcgcc
     3361 agcccgtgga aggtgccgac ccacatgccg ccctggctgg tgcccatcag ttgcccgata
     3421 cgatgacgca tctccgccgc cgctttgttg gtaaacgtca cagccataat cgagtatggc
     3481 gagcagtttt ccacgctcat cagccaggcg atacgatgca ccagtacgcg cgtcttacca
     3541 ctgcccgcgc ccgccagcac cagaaggttg ctgcgtggcg cggccaccgc ttcgcgctgt
     3601 ttgtcattaa ggctgtcgag caggtaagaa acgtccattg gcaccgccgc gtaaaaatag
     3661 gttgggcgga gaagcgcaac gccctccgcc aaaaagctgg gtatatatac agatttactg
     3721 attatatcag cgaggtcaga gatgccaacc gggaaatttc cagatgcggc agtaaacggc
     3781 tgtcccaggt ttgcatcaga tcgccatttt ccggtctgat ccaacaagcc tgcattccgc
     3841 tgcgaattgc cccacccacg tcagtggtga gatcgtcccc aacatgtaag atctcgccga
     3901 tcggcacgtt gagtttttcc gcagccaaaa agtacatatc gctgaacggt tttgagcgcc
     3961 cgtgcgggcc agcgcgcagc acaaactcaa aataatcccc caaaccaaac agttccggct
     4021 gggcgttacc gttggtgatc gccaccagcg gccatttctt cgccagctgt tttaaggtgt
     4081 cgtgagtttg ctgcgggacg tcgattcggc tgcgccattt ggcaaagtta atcattgctg
     4141 cgtgtgcgcc tgcactggct tcttcggcac tcagcccggc gtcgagcatc gcttgttcaa
     4201 tcgaacgaaa acgccaacgc gtcacgtcgt gataaatctc gggttccgct tcccgtaccg
     4261 cctggcgcag gcgttgcaga tcttcattct ggaagctgcg cagcgccgga tgataatttt
     4321 gcacaaaggt aagcgcctct cgctcggtgc gcaaaatcac cggacggtta tcgtaaaggg
     4381 tatcatccag gtcaaaggtg agcgccgaga tgcgccccaa aggccggtaa aaacgcatta
     4441 tttcccccgt ttggcgcgtg gatgcgccgc atcgtacacc gaggcaaggt gttgaaaatc
     4501 aagatgagta tagatttgcg tggtggagag gttggcatga cccagcagct cctgcacacc
     4561 acgaagatcg ccgctcgact ccagcatatg cgtggcgaac gagtgacgta atttatgcgg
     4621 atgaacgtga ttattcagcc cttgttttat gccccattcg gcaaagcgtt tctgcacatt
     4681 acgcgcggag atacgcttgc ccagtttcga cagaaaaagc gcgtcgtctt cgctaccaaa
     4741 caggtcgcgc aaatcaagcc agtgctcaat ccacgccaca gcgttgcgac caatcggcag
     4801 gcggcgctct ttgctgcctt tccccattac ccacacttca ccagactcca ggtcgaggtg
     4861 tttgatatcc agccccacca gctcggaaag acgcagaccc gcgccgtaca tcacttccag
     4921 cattgcacgg tcgcgtacag cgaggggatc gttgatatca atgtccagca gccgattcat
     4981 atcgtcgacg tcgatatttt tcggcagatg acgcggcgct ttcggtgccg aaacaccttt
     5041 cgccgggtta gctttgagtt cgttctggct gaccagccag tcaaaaaagc tacgtagcgc
     5101 agaaagccgt aacgcgaggc ttgctgctcc cagcccttta cggcgactgc gtacagcaaa
     5161 attgcgcacc atcgtcacat cacattgctg ccagctttgc aggacgtttt cgctggcaaa
     5221 attgatgatc gcctcaagct gacgctggta gttaagcagg gttatcgggc taagctggcg
     5281 ctccacgctc agataacgta ggtagcgttc tacatcggtg tgtaaatcgg tcatacgcgt
     5341 tcaatccaac gctccagaag ctccggcaac atcagcgcaa tttcatgaag taactgcgtt
     5401 ccctgccctt gttgatagtg actggcatcg cgactggtaa acagcacgac acccaaatca
     5461 gcatcgcttc ccagcatcga catcgccacc gatcccaccg ctttcgcttc cggtagcacc
     5521 accagcagct ctggtccgtt aagcggccca agatagtgct gttcctgccc caaacgctga
     5581 atacgcagcg gttcgaaaga ctgacggctt aatgccagat gagtgtggtt cgacggcgca
     5641 cctaagcgcc agcgatccgg aaacaggcgc agactcgcac ctgccaggcc gagatcgcgc
     5701 gcccagcggt gaaagcgcat cagcatatcg tcgagactgc tggcggcggt gagactgcgc
     5761 tgcaggtaga gtaggcgata aaacaggcct tcgttggcga tagcctgttc catcaacagc
     5821 gccatgttct cttccagaac atgaatatga ttacgtgcgc gggccatgtg ccactcgacc
     5881 aacgaaacgg tgccgcgtac cggatgcggc acacgtatcg cttctactgc gcgcgcatta
     5941 cggataaaaa actcaggatt tttaatcaga taatcgacaa ccgcccggtc atcaagctcc
     6001 gtgagtgttt cctgcagttc ttcccctggt tgcttcatag atgaataaat ccgtcgtaga
     6061 catgtaccgc cgggccagtc atatataacg ggtgacccgg acctttccag gcgatatcaa
     6121 gacgaccgcc ggggagttcc acgcgtactt cttcggccag caaaccttgc tgaatcccta
     6181 ctgcaaccgc cgcacacgcg ccgctgccgc aggcctgggt ttctcctgcc ccacgctcat
     6241 aaacgcgtaa acgaatatgc tcgcgcttaa ccacttgcat aaaaccgata ttggcgcgct
     6301 ccggaaaacg ctcgtggctt tccagaacag gaccaagcgt ttctaccgcc gcggtatcga
     6361 catcatcgac ctgaatcacg caatgcggat ttcccatcga caccacgccg cataagattg
     6421 tctgctcggc ggcgcgcata atataggtct tttccgcttt gttagcgcga aacggcacgg
     6481 cggaaggttc gaagttgggt tcgcccatat ttacgcggac cagatcatca tcggtgacgg
     6541 tcagaaccat ccgcccgttg gcggtgctga cgcggatatc acgcttattg gtcagtcctt
     6601 tcagacgcac aaaacgggca aagcagcgcg caccgttgcc gcactgcgcc acttcactgc
     6661 catcagcatt gaaaatgcga tagtgaaaat ccagttcagg atcatacggc ggctcaacca
     6721 ccagcagttg gtcaaaccct acccccaggt gtcgatcagc caggcgacga atcagctccg
     6781 gtgaaaaaaa gacattctgc gttaccgcgt cgacgaccat aaaatcgttg ccaaggccat
     6841 gcattttcga gaactgcatc atttactcca atcacgcggg tacagaaact gacttttagt
     6901 aattcacctg ggatggacca tcgccagtgg cgcgatcgtt tttatccggc accgtggatt
     6961 gcgtttgcgt ctctaccggt ttggtcggcg gcggtgcgtt tttatctgca ggcgggaaat
     7021 agagcggacc tttcagaccg cagcccgtca ggctgaagag agtaagtaat acagtgagtg
     7081 ccttaaacac gtttttcatt atggattgcc tgtaaagttc at
//
LOCUS       DAZPEP010000191         7075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZPEP010000191 DAZPEP010000000
VERSION     DAZPEP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.192"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(31..407)
                     /gene="rnpB"
                     /locus_tag="ACHUT9_003845"
     ncRNA           complement(31..407)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHUT9_003845"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(440..1585)
                     /gene="garK"
                     /locus_tag="ACHUT9_003846"
     CDS             complement(440..1585)
                     /gene="garK"
                     /locus_tag="ACHUT9_003846"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFR1350217.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1682..2572)
                     /gene="garR"
                     /locus_tag="ACHUT9_003847"
     CDS             complement(1682..2572)
                     /gene="garR"
                     /locus_tag="ACHUT9_003847"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFR1350218.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2602..3372)
                     /gene="garL"
                     /locus_tag="ACHUT9_003848"
     CDS             complement(2602..3372)
                     /gene="garL"
                     /locus_tag="ACHUT9_003848"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFR1350219.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3388..4722)
                     /gene="garP"
                     /locus_tag="ACHUT9_003849"
     CDS             complement(3388..4722)
                     /gene="garP"
                     /locus_tag="ACHUT9_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFR1350220.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5097..6668
                     /gene="garD"
                     /locus_tag="ACHUT9_003850"
     CDS             5097..6668
                     /gene="garD"
                     /locus_tag="ACHUT9_003850"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFR1350221.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6817..>7075
                     /gene="prlF"
                     /locus_tag="ACHUT9_003851"
     CDS             6817..>7075
                     /gene="prlF"
                     /locus_tag="ACHUT9_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFR1350222.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNNPQKTRP"
BASE COUNT         1746 a         1930 c         1741 g         1658 t
ORIGIN      
        1 aacccgccga agcgggtttt tacgtaaatc aggtgaaact gaccgataag ccgggttctg
       61 tcgtggacag tcattcatct aggccagcaa tcgctcactg gctcaagcag cctacccggg
      121 ttcagtacgg gccgtacctt atgaacccct atttggcctt gctccgggtg gagtttaccg
      181 tgccacggac tgttaccagc cgcgcggtgc gctcttaccg caccctttca cccttacctg
      241 atcccgcttg cgcgggccat cggcggtttg ctctctgttg cactggtcgt gggtttcccc
      301 cccaggcgtt acctggcacc ctgccctatg gagcccggac tttcctcccc tccgcccgtc
      361 tcccccgaag aggacgacga cgaagcggcg actgtctggt cagcttcggc gcgcagtata
      421 gagggtttgc gcgcccttgt caccccgcgt tgcgcattcc aatcgccagt gtcgcggcga
      481 tattacgtga agcacggcag atattgtcat aagccccgcg gaatgcttcg tccaacgtac
      541 ctatgctggt caatacgctg aagaccgcat cgatgccatg ctgatgtaca acgccaacat
      601 catcggtcag gctacccgca atgccaatca ccggtttatg gtacttcttc gccacgtttg
      661 caacgccaat cggtaccttg ccgtgaatac tctggctgtc aatacgccct tcaccggtga
      721 tcaccagcgt acaatcgtga atatgttctt ccagattcag cgccgtagtg acgatttcaa
      781 taccactttt cagttccgca ccaagaaacg ccatcagcgc cgcgcccata ccgcccgcag
      841 ctcctgcgcc ggggacatct ttcacatcaa catgcagcgc ttttttaatg acatcggcat
      901 agtgagagag gttattgtcc agctcaacaa tcatcgcttc actggctccc ttttgtgggc
      961 caaagatgcg cgatgcgccg ttatcgccca ccagcggatt ggtgacatca caagcgacgc
     1021 gaatgacgca atcttttaag cgcggatcga ggccggaaat atcaatatca ttcagagtat
     1081 taagactacc gccgccaaaa ccaatttcat tgccgttggc gtcgcataat ttcgccccca
     1141 gcgcctgtac catgcctgcg ccgccatcat ttgtagcgct gccgccaatg ccgataataa
     1201 tgtttgttgc actgctctcc agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg
     1261 ttacgagtgg atcgcgtttt tccgcaggta ccagctccag cccactggcc gccgccattt
     1321 caataaacgc ggttttgcca tcgccggaga tcccccaact ggcattcact ttctcgccca
     1381 gcggccctgt aacccaggcg tgacgttcag ccccctgggt ggctgcaatc atcgcttcca
     1441 ccgttccttc accaccgtcg gcaaccggaa cagaaacata ctgtgcatca ggaaaaattt
     1501 cccgaaatcc tttttctatc gcctgcgcaa cctcgctggc agataaactt tctttataag
     1561 agtctggggc gattacgatt ttcataccta tgcctgttac cacatgacgc cggagggcgt
     1621 ttctcttatt cggcctagat tccaggcccg gattgcaata caccatccgg gcacgacgtc
     1681 attaacgagt aacttcgact ttcgccagtt tttcgtagta gcacgccagg gcgctatgat
     1741 ccgccgttcc taaaccatct gcacgcagtg cctgcatcat ctccataacc gcagctgtga
     1801 gcagcagttg tgcgccgacg ccgtgagaag tatccagcgc attcgccaga tccttaatat
     1861 gcagatcaat gcggaagccc ggcttgaagt tgcggtccat caccatcggc gctttggcat
     1921 ccagcacggt actgcccgcc agcccaccgc gaattgcctg ataaaccagg tccgggttaa
     1981 cgcccgcttt ggttgccagc gttaacgctt ctgacatcgc agcaatattc agcgccacaa
     2041 tgacctgatt tgccagtttg gtgacgttac ctgcaccgat ttccccggta tgcaccacgg
     2101 aacccgccat cgctttcatc aaatcatagt atttgtcgaa aatagccttg tcgccgccca
     2161 ccatcactga cagcgtaccg tcgatggctt tcggttcacc gccgctcacc ggagcatcca
     2221 gcatatcaat gcctttcgct ttcagcgctt cgctgatttc acggcttgcc agcggtgcga
     2281 tagaactcat atcgatcaat accgtacctg gcttcgcgcc ttcaataatg ccattctcac
     2341 ccagcgccac ctctttcaca tgaggggagt ttggcagcat ggttatgatg acgtcgcact
     2401 gttcagcgat cgctttagcc gtagacgctg tttctgcacc tgcagcaatc acgtcagcaa
     2461 tagcttctgg gttacggtca gcaaccacca gcgagtaacc tgctttcaga aggtttttac
     2521 tcattggttt acccataatc cccaggccaa taaaaccaac tttcatagtc atatcaacca
     2581 tctctcttgt tgcggtggtg gttatttttt aaaggtatca gccagtttct gagtggcaga
     2641 gcggaagacg ccaagatcgc tgccgacagc cacaaacgtc gcgccccatt ccagataacg
     2701 acgcgcatcg gcttcgaccg gcgcgagaat accgctgggt ttgccgtgcg cgctggcacg
     2761 gttaaaaata tgctgaattg ctttttgtac atccggatgc gaggcattgc cgagatggcc
     2821 caatgccgca gccagatcgc tggggccgac gaagataccg tctacacctt cggtagcggc
     2881 aatagcatcg acgttatcta cgccctgctg actttctatc tggaccagaa tagtaatgtt
     2941 cttgttcgac tgagcgaaat aatccgccac ggtgccaaac atattggcgc ggtgagaaac
     3001 ggagacgccg cgaatgcctt ccggcgggta acgagttgat gccaccgcct gctctgcttc
     3061 ctcttttgtt tctacaaaag gaatcaggaa gttatagaaa ccgatatcca gaagacgctt
     3121 aataattacc ggctcgttgg tcggcactcg cactactggc gcgctggcgc tgcctttcag
     3181 ggccattaac tgcggaataa acgtggaaat atcgtttggc gcatgttcgc catccagcac
     3241 cagccagtca aacccagcca aaccaagaac ttcagtgcta atcgggttag agagtgctga
     3301 ccagcaacca atttgtacct gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac
     3361 atcgttattc atcgcttata cctttgctta tttctgcaat tccatacgtt taatgtcgcc
     3421 aactacgaag aggtagcaga ccatcgccat cagcgctgaa catcccacga aaaccagtgc
     3481 tgcattgaag gagtgcagtt cacttaccag gtagccaatc accagtggag tgacaatgga
     3541 ggcaacattg ccaaagacgt taaagacgcc gccacagagg ccaacaatct ctttcggcgc
     3601 ggtgtcagaa atcaccggcc agcccagagc accaaatcct ttgccaaaaa aagccagcgc
     3661 catcagcatg accaccagcg tggtgttgtt ggtgtagtta cataagatga tggtggaaac
     3721 cagcaacatt cccagcacaa tcggtagctt acgtgccagg gtcagggata aaccgcgttt
     3781 gatcagataa tccgagaaga cacctcccag cacgccgccc gcaaaaccac acagtgccgg
     3841 aatcgaggcg accagaccca ctttcagaat cgacatgcct ttttcctgca ccagataaat
     3901 cgggaaccag gtgaggaaga accaggtgat ggtgttgata aaatattgtc cgaaaaatac
     3961 gcccaacatc atgcggttag agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc
     4021 tgccgcgctg cccggctttt tgtggtccat atcgaccacc gcgccatttt cagagataaa
     4081 cttcagctct tccgcagaca tacgtgggtg atctgtcggg ttatgaatca acttgatcca
     4141 cagcgccgtc agcacaaaac caatcccccc cataacggtg aagacgtgct cccagcccca
     4201 agcgaaagtc agccagccaa gcagcggcga aaagagcgcc agcgagaaat attgcgccga
     4261 gttaaagatg gcggaggcag taccacgttc tttcgtcggg aaccaggcgg cgacaatacg
     4321 ggcgttcgcc gggaatgatg gcgcttccga gaaccccagc ataaagcgca taaagaacat
     4381 ggagatccct gcccaggcca gcgggaacat atcaacaaag ccttgcagga aggtgaacag
     4441 cgaccagaaa aagaggctgt aggtgtaaac ttttttcgag ccaaacttat caagcaacca
     4501 gccgccgggg atttgcatca gcaagtaggc ccagccaaaa gcagagaaga tgtaacccat
     4561 cgaaaccgca cttaactgca actcttttgc cacttcggta ccagcaatag acagcgttgc
     4621 acgatcggcg tagttaacgg cggtaacaat aaaaataatc agtaatatta aatagctggt
     4681 atgcacgcct ttctttttta cgtcaacggt gtccagaatc attttattta cctcgggtac
     4741 ttatgctgat ttttattatt atggggaagg tgttatttat gagttttatt tatgccgtta
     4801 cgacaatgaa ctcgggaatt agtataagca gcgggagaat aataatcatt gtgcaaatgc
     4861 taatttaatc agtactattt aaatattatt ttgagcatat gcacataagg ttgcgcgcta
     4921 aagcacagat ttgcgcttta ccttaccggg cggcactgca atccctgaaa tgattgacat
     4981 tgatcacatt tctgcgttta aactcctgac attcttattt cacccaatga agtcatttat
     5041 ttttaaatga gaccagatcc tcattttaat aacccctggc tggagaatat tgcacaatgg
     5101 ccaacatcga aatcagacaa gaaacgccaa ctgcgtttta tataaaagtt cacgacacag
     5161 ataatgtggc aattattgtt aatgataatg gcctgaaagc aggaacgcgt tttccggatg
     5221 ggctggaatt aattgaacat attccccagg ggcataaagt cgcattgctg gacattccag
     5281 ctaatggtga aattattcgt tatggcgaag tgattggtta cgccgtgcgc gcaatcccac
     5341 gcggaagctg gatcgacgaa tcaatggttg tactaccgga agcgccgccg ttacacacgc
     5401 tgccactggc aaccaaagtc ccggccccct taccgccgct ggaaggatac acctttgagg
     5461 gctatcgcaa tgccgatggc agcgtgggca ccaaaaacct gctcggcatc accaccagcg
     5521 tccactgtgt ggcaggcgtg gtggactatg tagtaaaaat cattgaacgc gatctgctac
     5581 cgaaataccc gaacgtcgat ggcgtggtgg ggctgaatca tttgtacggc tgtggcgtgg
     5641 cgattaacgc accggcggca gttgtgccta ttcgtaccat ccacaatatt tcgctgaacc
     5701 ctaactttgg cggcgaagta atggtgattg gtctgggttg tgaaaagttg cagcctgagc
     5761 gcctgctggt cggcacggat gatgtgcaag ctattccagt agaaagcgcc agcattgtca
     5821 gtttgcagga tgaaaagcat gtcggctttc agtccatggt cgaggatatt ttgcaggtcg
     5881 ccgaacgcca tctacaaaaa ctgaatcaac ggcagcgaga aacctgcccg gcttcagaac
     5941 tggtcgttgg tgtgcagtgc ggtggcagcg atgcgttttc tggtgtaacg gcaaacccgg
     6001 cggttggcta tgcgtctgat ctactggtgc gctgcggcgc aacggtgatg ttttcagaag
     6061 taacggaagt gcgtgacgcg atccatctgc tgacaccacg cgcagtgaac gaagaggtcg
     6121 gcaaacggct gctggaggag atggagtggt acgataacta tctcaatatg ggaaaaaccg
     6181 accgcagcgc caacccttcg ccgggcaaca agaaaggcgg tctggcaaat gtggtggaga
     6241 aggcactcgg ctccattgct aaatcgggta aaagtgcaat tgttgaagtg ctgtcgcccg
     6301 gtcaacgccc gactaaacgc ggattaattt acgccgcgac gccagccagc gattttgtct
     6361 gtggcacgca acaggtggct tcgggtatca ccgtacaagt gtttacgacc ggccgtggta
     6421 cgccgtacgg cctgatggcg gtacccgtaa ttaaaatggc aacccgcacc gagctggcga
     6481 accgctggtt tgatttaatg gatattaacg cgggcactat cgctaccggc gaagagagca
     6541 ttgaagaggt gggctggaag ttgttccact ttattctcga cgtcgccagc gggaagaaga
     6601 aaaccttctc ggatcaatgg gggctgcata accagctggc ggtgtttaac ccggcaccgg
     6661 tgacctgatt tcttttggaa cgtccctcgc aaaacatggc cttagtgcca tgtttttatt
     6721 gtttaaagcc cccacgtcca ttaataatgc atttgcatta ccttagttca agcttataat
     6781 ttgagcagaa aacaggctgt aaaaggacaa tgaatcatgc ccgctaatgc tcgctctcac
     6841 gctgtactga ccactgaatc aaaggtcacg atacgcggac aaacaactat ccccgcgcca
     6901 gtgcgtgagg ccttaaaact gaagccaggc caggacagca ttcattacga aattctgcct
     6961 ggtgggcaag tatttatgtg ccgactggga gatgaacagg aggatcatac tatgaatgca
     7021 tttttgcgtt ttctggatac agatatccag aacaacccgc aaaaaactcg tccat
//
LOCUS       DAZPEP010000192         7035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZPEP010000192 DAZPEP010000000
VERSION     DAZPEP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.193"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..245
                     /gene="yafQ"
                     /locus_tag="ACHUT9_003852"
     CDS             <1..245
                     /gene="yafQ"
                     /locus_tag="ACHUT9_003852"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFR1350223.1"
                     /translation="SKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSW
                     KGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            401..1141
                     /gene="dpaA"
                     /locus_tag="ACHUT9_003853"
     CDS             401..1141
                     /gene="dpaA"
                     /locus_tag="ACHUT9_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFR1350224.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1112..1879)
                     /locus_tag="ACHUT9_003854"
     CDS             complement(1112..1879)
                     /locus_tag="ACHUT9_003854"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFR1350225.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SQADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2085..2663)
                     /gene="lpcA"
                     /locus_tag="ACHUT9_003855"
     CDS             complement(2085..2663)
                     /gene="lpcA"
                     /locus_tag="ACHUT9_003855"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFR1350226.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2903..5347
                     /gene="fadE"
                     /locus_tag="ACHUT9_003856"
     CDS             2903..5347
                     /gene="fadE"
                     /locus_tag="ACHUT9_003856"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFR1350227.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5390..5836)
                     /gene="ivy"
                     /locus_tag="ACHUT9_003857"
     CDS             complement(5390..5836)
                     /gene="ivy"
                     /locus_tag="ACHUT9_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFR1350228.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6017..6787
                     /gene="yafV"
                     /locus_tag="ACHUT9_003858"
     CDS             6017..6787
                     /gene="yafV"
                     /locus_tag="ACHUT9_003858"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFR1350229.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            complement(6829..>7035)
                     /locus_tag="ACHUT9_003859"
     CDS             complement(6829..>7035)
                     /locus_tag="ACHUT9_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1350230.1"
                     /translation="WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGL
                     RRKMRKAAMDRNYLASVLAESGLS"
BASE COUNT         1642 a         1791 c         1882 g         1720 t
ORIGIN      
        1 tttcaaagga tgtaaaactt gcacaaaagc gtcataagga tatgaataaa ttgaaatatc
       61 ttatgacgct tcttatcaat aatgctttac cgcttccagc tgtttataaa gaccacccgc
      121 tgcaaggttc atggaaaggt tatcgcgatg ctcatgtcga accggactgg atcctgattt
      181 acaaacttac cgataaactt ttacgatttg agagaactgg aactcacgcg gcgctctttg
      241 ggtaaagcac gctgccgatg aaatttgtaa accctgcgcc gacaggcttg cgtaaaactc
      301 ttttggtgat aatgtgagcc ttccaaatcc agactattcc gatttgggat ctattttgcc
      361 gtcctggcgt gtgtaaccgt tttatcaagg aataagcagt atgcgtaaaa tcgcattaat
      421 tcttgcgatg ttgttgatcc cgtgcgtttc gtttgccggt ttgctgggca gcagtagctc
      481 gaccacgcca gtgagcaaag agtataagca gcagttgatg ggatcccctg tctacatcca
      541 gatcttcaag gaagaacgta cgctcgatct ctacgtcaaa atgggcgagc aatatcagct
      601 gctcgacagt tataaaatct gtaaatattc cggcggctta gggccaaaac agcgtcaggg
      661 cgatttcaaa agcccggaag ggttttatag cgtccagcgt aatcagttaa aaccagacag
      721 ccgttactac aaagcgatta atattggttt ccccaatgcc tatgaccgtg cgcatggtta
      781 tgaagggaaa tacctgatga tccacggcga ttgtgtttcc atcggctgct acgcaatgac
      841 caatcagggt attgatgaga tcttccagtt cgttactggt gcgctggtgt ttggtcagcc
      901 gagcgtgcaa gtgagtattt acccgttccg catgaccgac gccaatatga agcgccataa
      961 atattccaac tttaaggact tctgggagca actgaagccg ggttacgact actttgagca
     1021 aacccgtaag ccaccgaccg tttctgtggt caatggtcgt tacgtggtca gcaagccgtt
     1081 aagccacgaa gttgtgcagc cacaactggc atcaaactac acgctccccg aggcaaaata
     1141 agcgccattc gcctggcata atcttttgcc aggtttcatt gcccgtcagc ggctgtgttg
     1201 caatcaccgt gaccacatca ttcggtgtgg tctgcgagct gaagtcgatt tccacatcct
     1261 gatccagcag cgttgccacg ccaaacggcg cgcggcgggt gatccagtgt aaattagtcg
     1321 agcaatacgc catcacatag cgcccgtcag aaagcagcat gttgaaaacg cccttctgcc
     1381 gcagttcatc cgcctgtgag gcgatatatt taaacactgc cgccatgttg cccggtgtgc
     1441 gcgggtaacg ctgcgttaat ttatgcagga gccagcagaa ggctttttcg ctatcggttt
     1501 caccgaccgg gcggaagttg ccggtttcca gtgatttgta gcccgtcagt tgtccgttat
     1561 gggcataagt ccagttacgc ccccataact cacgggtaaa cgggtgagta ttttccagcg
     1621 ccacctcgcc ccgattggcc tggcgaatat gagccaccac cgaacaggat tttatcgggt
     1681 agtcctggac aagtttggcg atgggggaat taaagctggg ttgtggatct ttaaatgtgc
     1741 gacagccttt accttcgtaa aaggtaatgc cccagccatc tttatgtggc ccggttccac
     1801 caccacgctg caccagaccg gtgaaactaa agcagatatc ggtagggacg ttggcgctca
     1861 tcccgagcaa ttcgcacatg ggtaacctcc acaacagcgg gagcgcatgg cccccggcga
     1921 agattgattt aagaattttc aggccggatg cggcgtaaac gtcttattcg gcctacggtt
     1981 cgcgtacaga tttgtaggca tgataagacg catcagcgtc gcatcaggca tcagcgcaca
     2041 agtgccggat gcggcgtaaa cgccttatcc ggcctacgcc agacttactt aaccatctct
     2101 ttttcaatta actggatcag gatatggatc actttaatgt gaatctcctg aatgcggtcg
     2161 gcataaccaa agtgcggaac gcgaatttcg atatccgccg tgccagccat tttaccgccg
     2221 tctttaccgg tcagggtgat cactttcatc cccttctcac gcgccgctgc gatcgctttg
     2281 atcacgtttg cagagttacc ggaggtggag atccccagca gtacatcgcc ttcgcgacct
     2341 accgcttcaa cgtagcggga gaaaatatca ttgaaaccga aatcattacc gacgcaggaa
     2401 atatgactaa cgtcagaaat agcaatcgcc gggtagcccg gacggttttc acggtagcga
     2461 ccggtcaact cttcggcaaa gtgcatagcg tcgcaatggg aaccgccgtt gccgcaggaa
     2521 agcactttgc caccggcttt aaagctgtct gctaacagga ccgccgcgcg ctgaatggcg
     2581 tgaatattgg cgtcatcttt taaaaagtta gccagcgttt ccgccgcttc gttcagttcg
     2641 ttacgaataa gatcctggta catgaggata tccttcagca taaatgtaat agacaaaatg
     2701 cagtgtaccg gataccgcca aaagcgagaa gtacgggcag gtgctatgac caggactttt
     2761 tgacctgaag tgcggataaa aacagcaaca atgtgagctt tgttgtaatt atattgtaaa
     2821 cacattgcta aatgttttta catccactac aaccatatca tcacaagtgg tcagacctcc
     2881 tacaagtaag gggcttttcg ttatgatgat tttgagtatt ctcgctacgg ttgtcctgct
     2941 cggcgcgttg ttctatcacc gcgtgagctt atttatcagc agtctgattt tgctcgcctg
     3001 gacagccgcc ctcggcgttg ctggtctgtg gtcagcgtgg gtactggtgc ctctggccat
     3061 tatcctcgtg ccatttaact ttgcgcctat gcgtaagtcg atgatttccg cgccggtatt
     3121 tcgcggtttc cgtaaggtga tgccgccgat gtcgcgcact gagaaagaag cgattgatgc
     3181 gggcaccacc tggtgggagg gcgacttgtt ccagggcaag ccggactgga aaaagctgca
     3241 taactatccg cagccgcgcc tgaccgccga agagcaagcg tttctcgacg gcccggtaga
     3301 agaagcctgc cggatggcga atgatttcca gattactcat gagctggcgg atctgccgcc
     3361 ggagttgtgg gcgtacctta aagagcatcg cttcttcgcg atgattatca aaaaagagta
     3421 cggcggactg gagttctcag cttatgccca gtctcgcgtg ctgcaaaaac tctccggcgt
     3481 gagcgggatc ctggcgatta ccgtcggcgt gccgaactca ttaggcccgg gcgagctgct
     3541 gcaacattac ggcactgacg agcagaaaga tcactatctg ccgcgtctgg cgcgtggtca
     3601 ggagatccct tgctttgcac tgacaagccc ggaagcgggt tccgatgcgg gcgcgattcc
     3661 ggacaccggg attgtctgca tgggcgaatg gcagggccag caggtgctgg ggatgcgtct
     3721 gacctggaac aaacgctata ttacgctggc accgattgcg actgtgcttg ggctggcgtt
     3781 taaactctcc gacccagaaa agctgctggg tggcgcagaa gatttaggta ttacctgtgc
     3841 gctgatcccg accaccacgc cgggcgtgga aattggtcgt cgccacttcc cgctgaacgt
     3901 accgttccag aacggaccga cgcgcggtaa agatgtcttc gtaccgatcg attacatcat
     3961 cggtgggccg aaaatggccg ggcaaggctg gcggatgctg gtggagtgcc tctcggtagg
     4021 ccgcggcatc accctgcctt ccaactcaac cggcggcgtg aaatcggtag cgctggcaac
     4081 gggcgcgtat gctcacattc gccgtcagtt caaaatctct atcggtaaga tggaagggat
     4141 tgaagagccg ctggcgcgta ttgccggtaa tgcctacgtg atggatgctg cggcatcgct
     4201 gattacctac ggcattatgc tcggcgaaaa acctgccgtg ctgtcggcta tcgttaagta
     4261 tcactgtacc caccgcgggc agcagtcgat tattgatgcg atggatatca ctggtggcaa
     4321 aggcattatg ctcgggcaaa gcaacttcct ggcgcgtgct taccagggcg caccgattgc
     4381 catcaccgtt gaaggggcta acattctgac ccgcagcatg atgatcttcg gacaaggagc
     4441 gattcgttgc catccgtacg tgctggaaga gatggaagcg gcgaagaaca atgacgtcaa
     4501 cgcgttcgat aaactgttgt tcaaacatat cggtcacgtc ggtagcaaca aagttcgcag
     4561 cttctggctg ggcctgacgc gcggtttaac cagcagcacg ccaaccggcg atgccactaa
     4621 acgctactat cagcacctga accgcctgag cgccaacctc gccctgcttt ctgatgtctc
     4681 gatggcagtg ctggacggca gcctgaaacg tcgcgagcgc atctcggccc gtctggggga
     4741 tattttaagc cagctctacc tcgcctctgc cgtgctgaag cgttatgacg acgaaggccg
     4801 taatgaagcc gacctgccgc tggtgcactg gggcgtacaa gatgcgctgt atcaggctga
     4861 acaggcgatg gatgatttac tgcaaaactt cccgaaccgc gtggttgccg ggctgctgaa
     4921 tgtggtgatc ttcccgaccg gacgtcatta tctggcacct tctgacaagc tggatcataa
     4981 agtggcgaag attttacaag tgccgaacgc cacccgttcc cgcattggtc gcggtcagta
     5041 cctgacgccg agcgagcata atccggttgg cttgctggaa gaggcgctgg tggatgtgat
     5101 tgccgccgac ccaattcatc agcggatctg taaagagctg ggtaaaaacc tgccgtttac
     5161 ccgtctggat gaactggcgc acaacgcgct ggcgaagggg ctgattgata aagatgaagc
     5221 cgctattctg gtgaaagctg aagaaagccg tctgcgcagt attaacgttg atgactttga
     5281 tccggaagag ctggcgacga agccggtaaa gttgccggag aaagtgcgga aagttgaagc
     5341 cgcgtaattg aataacggag ccgaaaggct ccgtttcttt atccgctaat tatttaaaat
     5401 taaagccatc cggatggttt tccaggctgc cggtcaacgc cgcgaacagc accgttttac
     5461 catcaatcga aagcgcatcg ttcacattca gccaggtgag tttctcttgc gacgttttct
     5521 catcaatagt cgagaacagc cccgtcatct gattagattt ctcggaccac atcacagcga
     5581 tacgttgcga gccacagtca tgcggtttgc acgcgctcat cacctgatac gtatcatctc
     5641 ccaacgttac ggtttgtgcg ggagtataag taccgccttt catcacccag gcaggcagct
     5701 tatgcccttg taccatctga ttaaatgcag ctttggtggt ttcgcccttt gcaaggctgc
     5761 taatggttaa atcatcctgc gccattgcac tggtggcgat gaccagagcg gcgactgtcg
     5821 ttattgtctt aaacatcatt cctcccgagc ttatcctgcc catgttatta accttctaaa
     5881 gcctggcgat aacacgataa tttgcaaatc agatatttcc aatatcagca tttgttatca
     5941 atttattctg cgctaaggca actgcctgat aaggtgttaa agttataaaa cccttcgcat
     6001 aaaggagttg tacaccgtgc ctggtttgaa gattacgctt ttgcagcaac cactggtgtg
     6061 gatggatggc cctgccaacc tgcgtcattt tgatcgtcaa ctggaaggta ttaccgggcg
     6121 cgatgtgatc gttctaccga agatgtttac cagcggcttt gccatggaag cggcagcttc
     6181 gtcgctagca caagatgacg tagtggactg gatgacagct aaggcgcagc agtgcaatgc
     6241 gctgattgca ggcagtgttg cattacaaac ggagtctggt tcggttaacc gctttttgct
     6301 ggttgagccg ggcggcacgg tacattttta tgataagcgt catctgttcc gcatggcaga
     6361 tgagcatcta cattataaag cgggcaatgc gcgagtgatt gtggaatggc gcggctggcg
     6421 tattttgccg ttggtgtgct acgacttacg ttttccggtg tggtcgcgca atctcaacga
     6481 ttatgacctc gccatctacg ttgccaactg gcctgctccg cgctctctgc actggcaggc
     6541 attgctgacg gcccgcgcga ttgagaatca ggcgtatgtg gcgggatgca atcgcgtcgg
     6601 cagcgatggc aacggctgcc attatcgcgg tgacagccgg gtgattaatc cacaaggcga
     6661 gattatcgct actgccgacg cgcatcaggc aacgcgcatt gatgcggagc tgtcgatgat
     6721 ggcattgcgg gaatatagag aaaagtttcc ggcatggcag gatgcggatg agtttaggtt
     6781 gcggtgagtg ggggttgtgt ttaaggctgg gggagagtcg gggtaagact acgaaagccc
     6841 gctctccgca aggactgacg ccagatagtt tctgtccata gctgcttttc gcatcttacg
     6901 tcttaaccct gccttgaata ccttatcatt cgtcaaaata ttaatagcga tgtgccgtat
     6961 ccctgaaaat aattctgctg catttcctct tcttattttg cagtcgtctt cattcattac
     7021 cacgtccaga cgcca
//
LOCUS       DAZPEP010000193         7034 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZPEP010000193 DAZPEP010000000
VERSION     DAZPEP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7034
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.194"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..2633)
                     /locus_tag="ACHUT9_003860"
     CDS             complement(<1..2633)
                     /locus_tag="ACHUT9_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1350231.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAADSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTLETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAANSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIIQLSSATNSTSETLAATPKAVKAANDNAEKRLQKDQNGADIPGKDTFTKNIGAC
                     RAFGGSVSTTTGNWTTAQFIEWLDSQGAFNHPYWMCKGSWSYGNNKIITDTGCGNIHL
                     AGAVIEVMGIKSAMTIRITTPTTSSGGGTTNAQFTYINHGTDYSPGWRRDYNSRNKPT
                     ASEIGALPSGGTAVSSVNLASKGRVAALTDNTQGAAGLELYEVYNNGYPTAYGNIIHL
                     KGMTAVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVG
                     APIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVL
                     SQEQDGIKSHTHSASASSTDLG"
     gene            complement(2698..3214)
                     /locus_tag="ACHUT9_003861"
                     /pseudo
     CDS             complement(2698..3214)
                     /locus_tag="ACHUT9_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            complement(3285..6782)
                     /locus_tag="ACHUT9_003862"
     CDS             complement(3285..6782)
                     /locus_tag="ACHUT9_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFR1350232.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REVTLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            complement(6843..>7034)
                     /locus_tag="ACHUT9_003863"
     CDS             complement(6843..>7034)
                     /locus_tag="ACHUT9_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350233.1"
                     /translation="TPRTQTTDNGKQNIYFSSLDNMVAQGNVMPVLYGEMRVGSRVVS
                     QEISTADEGDGGQVVVIGR"
BASE COUNT         1374 a         2116 c         1797 g         1747 t
ORIGIN      
        1 cccaaatccg tactggatgc gctggcgctg tgggtgtgcg atttaatgcc atcctgttcc
       61 tgagacaata cggcacgacc actggcgggc ttgcccttaa tcgtccagcc acgcatatca
      121 gggatcacgc ctgacggata agcggctgca agtttcgggt aagcagattt gtcaaaagtc
      181 tgcccctgca tcagggcata accagacgga acggtatctg atggccacgg gattggtgca
      241 ccgactggat aaaactctgc aggaggatga gccgaggtgt aaagctgcgc ccacggcgac
      301 cagtttgcgt cggtcgtatc ccgtcgtgaa cgaataaatg ccggagcatg agcaccgctt
      361 gtaccactcc agccgatgag taactcacct tcgccaacgg ctgtcatccc tttcaggtga
      421 atgatatttc catacgctgt tggatatccg ttgttataca cctcgtataa ctcaagacct
      481 gctgccccct gcgtattgtc tgtcagcgcg gctacccgac cttttgaagc cagattaact
      541 gatgatactg ctgttccacc tgacggtagc gccccgatct ctgatgccgt tggcttattt
      601 ctggagttat agtcccttcg ccagccaggt gaataatctg ttccgtgatt aatataggta
      661 aactgggcgt tggttgttcc gccaccgctg gatgtggtcg gagtggtaat gcggatcgtc
      721 atcgctgact ttatccccat tacttcaatg acagctccgg cgagatgaat attaccgcag
      781 ccagtatcag taatgatttt attattgcca taagaccagg aacccttgca catccagtat
      841 ggatggttaa atgctccctg agaatccagc cactcgataa actgtgcagt cgtccagttt
      901 cctgttgttg tgcttactga cccaccgaag gcacggcagg caccaatatt tttcgtaaag
      961 gtgtctttgc cagggatatc cgcaccgttc tgatctttct gcagacgttt ctcagcattg
     1021 tcattggctg cttttactgc ctttggcgtt gccgccagcg tttcagacgt gctgttggtc
     1081 gcgctgctta gctggattat ccctttctgt gctgtcgttg catcctgtgc ggtgtatttc
     1141 ccgttagcca ggtcatacgc ggccttaacg gcttttggtg ttgccgccag tgactcggaa
     1201 gtgctgttag tcgcactact gagctgtact atcccctttt tcgtcgtgct cgcatcctca
     1261 agcgccacgg cggatgcaat atcctctgcc cgttttgccg ctgtctcagc gcgcgttgct
     1321 gcagattccg ccgtactttt gctctgagct gctgccgtcg cactaccagc agcctctgtc
     1381 gccttcgtgg atgccgtcgt ggcgctgccc ttcgctgctg acgcctgtct ggtcgcctca
     1441 tcttttgaag cagacgccga tgatgccgat gacgccgccg aactggcgga cgatgcggca
     1501 gccgtttttg aggattctgc acgggtttcc gacgctttcg cgttcgtttc ggatgtcttc
     1561 gctgcggaag ctgacctcgc tgctgccgtg gcctgctcag tggcttcgcc agccttcgtt
     1621 gtggctgttg aagcggatga tgcggcgctt tctgccgatt ttccggcggc ggtggcactg
     1681 gctgaggcct gcccggcact tgttgacgcg gcgctggcag acgacgcagc cgctgttttt
     1741 gagcctgccg cagctgaggc actctgtccc gctgccgttt cagaagacct ggcgttcgtc
     1801 tcggacgttt tggccgcctt cgcagaattt gcagccgccg tcgccgagga agctgcgcta
     1861 ctggcgcttg atgatgcgtt cgtttctgat gattttgccg cctcttttga agccgacgca
     1921 tcccgggctg aggtggcagc ttctgacgct ttcgtggtcg cggtggatgc agaagtggct
     1981 gctgattgtt gtgacgctgc cgcattcgtt tctaacgttt tcgccgcacc ggcactggta
     2041 gccgccgcgc tttttgagga ctctgcagcg gcagcacttt ttgaggcttc agtggccttt
     2101 gttgatgccg ttcctgcgct ggaagacgct gactgagccg acgaagcggc ctgtccggct
     2161 gacgtgctgg ctgcacgtgc tgaatctgca gcatcagtcg catgggttgc cgcctcacgg
     2221 gctgatgtgc tggcatcgct ggctgatttc ttcgcggctg ccgtgttctg tgccaccgcg
     2281 gacgcgttac gcgccacctc ttccaccatc agctcaaaac ggcgcagtgc ctccggacgg
     2341 gcatcatcct ccgtcatggc accgagaaaa tcattcagcg taccgggtcg ggaatcttca
     2401 tacacggtga tggtcccggc atgtgacggc gggaatcctt ccaccaacag aataacgctg
     2461 tactgaccgt actcaacgtc catgctgtaa cgcccggctt catccggatt ttctgaggcc
     2521 agcgtgttca ccaccaccgt ggtgctgtta cgttttgctt tcagctggat tgtgcagttc
     2581 tgtaccggtt ttcctgtgcc gtctttcagt acacctgaaa tctttactgc catattcacc
     2641 ccacaaaaaa gcccgcctga accggcgggc tgtcataaca ctgtgttacc tggctaatca
     2701 gaatttataa ccgacaccca cgatgaaacc gtcagtgcgc cagagacgtg ttgctgtggc
     2761 gaccgtcatc acttccggtc agcacatcgt gcgttttccc cttattgtca gttacgcgga
     2821 tataatcccc ggaaaaagtc gacacacggc tgtaagccac acccgccatc gcatacgcgc
     2881 tgaaccattc attcacgcgc acagacggcc ccgccatcac gctgaaccag cggttacgca
     2941 cggaatcttc atgccagcgg gtatcgctgt aacgggtcag ctggcgattc ttgtctcctg
     3001 catagctgaa tgacgtcacc agccccagcg tatccgtaaa ctcataacgg tatttcacgt
     3061 taatcccgtt cagatcatca ctgccgggaa cgttggtccg ggcatgaaga taccccgcgc
     3121 tcagcgtgga ctgatgttca gacgcccatg caggcgcacc ggatacggcc aggcaaatgg
     3181 ctgcggacaa aatggctgca caaactttac gcataattac ctctcgcttt tctgcaataa
     3241 aaaaggcgtc attcctgacg ccatttattg gggttataaa tatttcaacg aatactgatg
     3301 ccggaagccg cttttttggt cacaatcacc gtacagtcgg tgatattgcc tgccccctga
     3361 ttgcctttct ggaaaatctt aaactccaga gtgacgctac caccaccact aggcatatca
     3421 ataactgcac tgtaactacc gggaatggca cctttagttt ctctggatgc gattaatacg
     3481 ccgtttttgc gaacttcaaa accataaccc gtgtatcgcg tgcctcctgg gttatttccg
     3541 ctccccggat cgtcatacgc tataccgtta aaaataatgg gcggaataat aatctggcgg
     3601 tcaaagttat gatcatcgct tatggtgact gtaaccgtcc cgtttggtgt ttccgtgtta
     3661 ccccacgtac cagcctgttt cggaaatgat ttggatacag ctttaacgaa gtcacctctg
     3721 acctgagtcg cctccagcat gcccttaatc gtacagtttt catttaccgt gacattgttg
     3781 agcgtcccgg cgttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt
     3841 ccgtccggag tcagggaaaa tgccgatgga tttccaccgc tggtaatggt gggggccgtc
     3901 aggcgtttca ggaacacgtc gttcatgaat atctgatcgc cctgaccaac aaacatcggt
     3961 tttgtgttgc cattcgcagg attaaccatc gcaatcctgt ccgccgccag cagcacctga
     4021 ctctgcatgc cgtcaggggt gttctcaata ccggcaccaa tacccgcgat ataaaggcgt
     4081 ccgtcctgca tctgctgcag cttcacagcc cacatgctgt tcaggttatt atttgtatca
     4141 acctgaacct tctgtatctg ctgaattgcc gcactctggt cttccagttt cttattgacg
     4201 gtctgcgtga tttcattgct gacatccgta atggacgtcc tgatttcagc caggtcaggc
     4261 gcaagctgac cgttatcaat ctgcgtccac agctcctgag ccagatgggt tttccctatc
     4321 tcgcctttga aaaaatccag ataaccggat gcatcatcac tcggctgacc aacagcctcc
     4381 acgaatgccg atttgccaac ggtgttcaca ctgcggatat aaaagtaata atcatggccc
     4441 ggtttgatat tgatactggc ggctatccag tacagcgccg tgccaagata gcgtgctgtg
     4501 gtttcaacct gcctgatatc cgcaatccgc ttttccgaga accagaactc aaactgtacc
     4561 gtcggatcat aaaccgcaag atgcggcgtg gcggttatct gaaaatagcc cggcgtcagc
     4621 tcaatccgcg acagcgctgc cggtgcggca atccggaacg ataccgacgc cggatcgccc
     4681 tgctgccccc acgcatttac tgcccggact gtcagcctgt agttccccag cgccagttgt
     4741 gtgaagcggt aagtggtttc cgtcgtccgg gccgtgctga ccagccgctc actgccgtca
     4801 tccgctgtta cggtcagacg gagcaggaag ctcacgccct tcaccacctt cggcgtgtcc
     4861 cagcgcgcca gcacctgata ttccccgctg tctgcggtga cttcggcagt caggtgctgc
     4921 accgctggcg gcgtgacacc attcaccgtg ccgctctggt cgccgtcaaa gtgcgccccg
     4981 ttatccacga tggcttcttt ttccggcaca tgctgcacgg cggtgatggc atacgtgccg
     5041 tcgtcgttct cacggatact cacgcagcgg aacaatcgct ggtgcagcgt cggcagcttc
     5101 agcccccaca cgctgtattc agcaacaccg tcaggaacac ggctcacttt taccttcacg
     5161 ccgtcggtga cggactggac ctccacgctg accggattcc cctgcccgtc aaccaggctt
     5221 atcagcgtgg tgccggagga tggcagcgtg acttcacggt cgagcgtcag cgtccgggtc
     5281 tggctgttca ccgccagcac gcgcccgccg gtgcggatac cggcatagtc atcatcgcag
     5341 atttcaatga catcgcccgg tacatgacga agcccttcag cacccacgct gaaatccacg
     5401 gtctgcgtct ccagcagttc cgttttaatc agccacagcc cggcgcggtg tgcctgcccc
     5461 cggctggtac agccaaaggc atccatcttc gtgacgttac gaccgtaacg gacaatggcc
     5521 tgcgtatcct ccacaagctc tgtcgccgtc tcccagccgt tatccgggtc aatccagttc
     5581 acctcaacgg cattatggcg gtctttcagg gcgctgaaac tgtagcggaa cggcgcgcca
     5641 tcatccggca tcaccacatt actgcggtta taggcccaca ccttatccga ctgtcggtcc
     5701 tgcacgaacg tcagcgtctg cccgttccat accggcatac agcgcatcgc cgagcagaaa
     5761 tcactgagca catcccacgc cttgcgctgt gtggtcagcc aggcattaca ggtgatgcgc
     5821 ggctccgtgc tgccaaagcc gtccggcacc gactggtcac agtactggcc gatgacatac
     5881 agcgcccatt tatccacatc tgccgcacca agacgtttcc ccatgccata gcgcggatgg
     5941 gtcagcatat cccacagaca ccaggccatg ttgttgctgt atgccggttt taacgttccg
     6001 tcccagatac cgctgtattg ccgcgtctgc ggattatagt tcgacggcac ctgcagaatg
     6061 cgcccgcgaa gatgataatt acggctcacc tgctggctgc cgaactgctc cgaatccacc
     6121 tgtacgccga ccagtgccgt gttcgggtag cactgtttca catcgatgat ttcggtgtat
     6181 gacgaccaga gcgttttgtt ctgcagctgg tctgtggtgc tgtccggcgt cattctgcgc
     6241 atccggatat taaacgggcg cggcggcagg ttatccacca ccaccgaggc cagatactgc
     6301 gaggtggttt tgcccttaat ggtgatgtct ttttccgtca cccagccacc gttacgctgt
     6361 atctgaacca gcaggcgaac ttcagacgga ttcctgtctc cctttgaggt ggtttccacc
     6421 agtgcctgta caccgaaggt aaaacgcagt cggtcaatgt ttgccgacgt gatggtccgg
     6481 gtgatcggcg tgtcatattt cacttccgta cccagcaccg tctcggagcc ggaggattca
     6541 aatccctccg gcggtgactg ctcctgctca cctgcccgga acaccaccgt gacgccggag
     6601 atgttggtat tcccctcact gtccagcacc ggcgtactgt tcagcagcac gctttttaat
     6661 ccatccaccg gaccttcaac cggcccttcg ctgatggcat cgatcacact cagcagctgc
     6721 gtggacttca ggttgtcctt cgcttcgcgc ggggtatgcc ccttactgct gcctttaccc
     6781 attcgtcatg ctccataaac gataaaaccg cccggaggcg atttcacata aaacattttg
     6841 catcagcgac caatcaccac aacctgacca ccgtcccctt cgtctgccgt gctgatctcc
     6901 tgagatacca cacgtgaccc cacgcgcatt tcaccataca gaacaggcat aacattgccc
     6961 tgggcaacca tgttatccag tgaggagaaa tagatgttct gcttaccgtt atccgttgtc
     7021 tgtgtgcggg gagt
//
LOCUS       DAZPEP010000194         6903 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZPEP010000194 DAZPEP010000000
VERSION     DAZPEP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.195"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..192
                     /locus_tag="ACHUT9_003864"
     CDS             <1..192
                     /locus_tag="ACHUT9_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1350234.1"
                     /translation="TPTAASTDNGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVS
                     QEISTADEGDGGQVVVIGR"
     gene            253..3651
                     /locus_tag="ACHUT9_003865"
     CDS             253..3651
                     /locus_tag="ACHUT9_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350235.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     AITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNAWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGNINANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            3718..4317
                     /locus_tag="ACHUT9_003866"
     CDS             3718..4317
                     /locus_tag="ACHUT9_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFR1350236.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVSG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSLRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAVDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            4382..>6903
                     /locus_tag="ACHUT9_003867"
     CDS             4382..>6903
                     /locus_tag="ACHUT9_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1350237.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSNTVVVNTLASENPD
                     EAGCYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEALKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAASSARDASASKEAAKSSETSAASSASSSASSATAAGNSAKAAKT
                     SETNAKSSETAAEQSASAAADSKTAAASSASAASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQASAAASSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAARGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTA
                     QKGIVQLSSATNSTSESQAATPKAVKAAYDLANGKYTAQDATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGYNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSAGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAVAYPSG
                     VIPDMRGWTIKGKPASGRTVLSQEQDGIKSHTHSASASSTDLG"
BASE COUNT         1703 a         1771 c         2067 g         1362 t
ORIGIN      
        1 acgcccacgg cagccagtac agataacggc aaacagaaca cgtatttctc gtcactggat
       61 aacatggttg cccagggtaa tgttctgcct gttctgtacg gggaaatgcg cgtggggtca
      121 cgtgtggttt ctcaggagat cagcacggca gacgaagggg atggtggtca ggttgtggtg
      181 attggtcgct gatgcaaaat gttttatgtg aaaccgcctg cgggcggttt tgtcgtttct
      241 ggagcgtgag gaatgggtaa aggcagcagt aaggggcata ccccgcgcga agcgaaggat
      301 aacctgaagt ccacgcagct gctgagtgtg atcgatgcca tcagcgaagg gccggttgaa
      361 ggtccggtgg atggattaaa aagcgtgctg ctgaacagta cgccggtgct ggacagtgag
      421 gggaatacca atatctccgg tgtcacggtg gtgttccggg caggtgagca ggagcagtca
      481 ccgccggagg gatttgaatc ctccggctcc gagacggtgc tgggtacgga agtgaaatac
      541 gacacgccga tcacccgcgc catcacgtcg gcaaacatcg accgactgcg ctttaccttc
      601 ggtgtgcagg cactggtgga aaccacctca aagggggacc ggaatccgtc ggaagttcgc
      661 ctgctggttc agatacagcg taatggtggc tgggtgacgg aaaaagacat caccattaag
      721 ggtaaaacca cttcacagta tctggcctcg gtggtggtgg ataacctgcc gccgcgcccg
      781 tttaatatcc ggatgcgcag gatgacgccg gacagcacca cagaccaact gcagaacaaa
      841 acgctctggt cgtcatacac cgaaattatc gatgtgaaac agtgctaccc gaacacggca
      901 ctggtcggcg tgcaggtgga ctcggagcag ttcggcagcc agcaggtgag ccgtaattat
      961 catctgcgcg ggcgtattct gcaggtgccg tcgaactata acccgcagac gcgacaatac
     1021 agcggtatct gggacggaac gttaaaacca gcatacagca acaacatggc ctggtgtctg
     1081 tgggatatgc tgacccaccc gcgctacggc atggggaaac gtcttggtgc ggcggatgtg
     1141 gataaatggg cgctgtatgt tatcggccag tactgcgacc agtcagtgcc ggacggcttt
     1201 ggcggcacgg agccgcgcat cacctgtaat gcctggttga ccacacagcg taaggcgtgg
     1261 gatgttctca gcgatttctg ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg
     1321 ctgacgttcg tgcaggaccg accgtcggat aaggtgtgga cctataaccg cagtaatgtg
     1381 gtgatgccgg atgatggcgc gccgttccgc tacagcttca gcgccctgaa ggaccgccat
     1441 aatgccgttg aggtgaactg gattgacccg gataacggct gggagacggc aacagagctt
     1501 gtggaggaca cgcaggccat tgcccgttac ggtcgtaatg tcacgaagat ggatgccttt
     1561 ggctgtacca gccgggggca ggcacaccga gccgggctgt ggctgattaa aacggagctg
     1621 ctggaaacgc agaccgtgga tttcagcgtg ggcgcagaag ggcttcgcca tgtaccgggc
     1681 gatgttattg aaatctgcga tgatgactat gccggtatca gcatcggtgg tcgcgtgctg
     1741 gcggtgaaca gccagacccg gacgctgacg ctcgaccgtg aaatcacgct gccatcctcc
     1801 ggcaccacgc tgataagcct ggttgacgga agcggcaatc cggtcagcgt ggaggtccag
     1861 tccgtcaccg acggcgtgaa ggtgaaagtg agccgtgttc ctgacggcgt tgccgagtac
     1921 agcgtgtggg ggctgaagct gccgacgctg cgccagcgcc tgttccgctg cgtgagtatc
     1981 cgtgagaatg acgacggcac gtatgccatc accgccgtgc agcatgtgcc ggagaaagag
     2041 gccatcgtgg ataacggggc gcactttgac ggcgaccaga gcggcacggt gaatggtgtc
     2101 acgccgccag cagtgcagca cctgaccgca gaagtcaccg cagacagcgg ggaatatcag
     2161 gtgctggcgc gctgggatac gccgaaggtg gtgaagggcg tgagctttat gcttcgcctg
     2221 accgtggccg cggatgacgg cagtgagcgg ctggtcagca cggcccggac gacggaaacc
     2281 acataccgct tcaggcagct ggctctggga aattacagtc tgacagtccg ggcggtaaat
     2341 gcctgggggc agcagggcga tccggcatcg gtatcgttcc ggattgccgc accggcagcg
     2401 ccgtcacaga ttgagctgac gccgggctat tttcagataa ctgccacgcc gcatcttgcg
     2461 gtttatgatc cgacggtaca gtttgagttc tggttctcgg aaacgcggat taccgatatc
     2521 aggcaggttg aaaccacagc ccgctacctt ggcacggggc tgtactggat agccgccagt
     2581 atcaatatca aaccgggcca tgattattac ttttatatcc gcagtgtgaa caccgttggc
     2641 aaatcggcat tcgtggaggc cgtcggtcgg gcgagcgatg atgcggaagg ttacctggat
     2701 tttttcaaag gcaagataac cgaatcccat ctcggcaagg agctgctgga aaaagtcgat
     2761 ctgacggagg ataacgccag cagactggat gagttttcga aagagtggaa ggatgccaac
     2821 gataaatgga atgccatgtg ggctgtcaaa attgagcaga ccaaagacgg caaacattat
     2881 gtcgcgggta ttggcctcag tatggaggac acggaggaag gcaaactgag ccagtttctg
     2941 gttgctgcta accgtatcgc gtttattgac ccggcaaacg ggaatgaaac gccgatgttt
     3001 gtggcgcagg gcaaccagat attcatgaac gacgtgttcc tgaaacgcct gacggcaccc
     3061 accattacca gcggtggcag tcctccggta ttttccctga catcagacgg aaagctgact
     3121 gctaaaaatg cggatatcag cggaaatatt aatgcgaact ctggcacgct caacaatgtg
     3181 acgattaatg agaactgtca gattaagggg aaactgtcag ccaatcagat tgaaggcgat
     3241 attgtcaaaa cggtcagcaa gtctttcccc cgcacgaaca gttatgccag tggcaccatc
     3301 acggtaagaa tcagtgatga tcagaaattt gaccggcagg tcatgatacc gccagtgtta
     3361 ttccgcggtg gtaagcatga gaatttcaac agtaataacc aacagtcata ctggtattca
     3421 acctgccggt taagagtgac ccgcaatggt caggagattt ttaatcagtc cacgacggat
     3481 gctcagggcg tattttcctc agttatagat atgcctgccg gacaggggac gctgacactg
     3541 acattcaccg tatcttcatc aggagcgaat aactggacac caacaaccag tatcagcgat
     3601 ctgctggttg tggtgatgaa aaaatccaca gcaggtatca gtatcagctg aattttataa
     3661 cccataacgg gcgtcagaaa tgacgccttt tttattgcag aaaagcgaga ggtaattatg
     3721 cgtaaacttt atgccgccat tttgtccgca gccatctgtc tggccgtatc cggtgcgcct
     3781 gcatgggcgt ctgaacatca gtccacgctg agcgcgggct atcttcatgc ctcgacgaac
     3841 gtttccggca gcgatgatct gaacgggatt aacgtgaaat accgttatga gtttacggac
     3901 acgctgggaa tggtgacgtc attcagctat gcaggagaca agaatcgcca gcttacccgt
     3961 tacagcgata cccgctggca tgaagattcc ctgcgtaacc gctggttcag cgtgatggcg
     4021 gggccgtctg tacgcgtgaa tgaatggttc agcgcgtatg cgatggcggg tgtggcttac
     4081 agccgtgtgt cgactttctc cggggattat ctccgcgtaa ctgacaacaa ggggaaaacg
     4141 cacgatgtgc tgaccggaag tgatgacggt cgccacagta acacctctct ggcgtgggga
     4201 gctggcgtgc agtttaaccc gaccgaatcc gtggccgttg atgtcgctta tgaaggctcc
     4261 ggcagtggcg actggcgcac tgacgggttc atcgttggtg ttggttataa attctgatta
     4321 gccaggtaac acagtgttat gacagcccgc cggttcaggc gggctttttt gtggggtgaa
     4381 tatggcagta aagatttcag gtgtactgaa agacggcaca ggaaaaccgg tacagaactg
     4441 cacaatccag ctgaaagcaa aacgtaacag caacacggtg gtggtgaaca cgctggcctc
     4501 agaaaatccg gatgaagccg ggtgttacag catggacgtt gagtacggtc agtacagcgt
     4561 tattctgttg gtggagggat tcccgccatc acatgccggg accattaccg tgtatgaaga
     4621 ttcccgaccc ggtacgctga atgattttct cggtgccatg acggaggatg atgcccgtcc
     4681 ggaggcactg cgccgttttg agctgatggt ggaagaagtg gcgcgtaacg cgtccgcggt
     4741 ggcacagaac acggcagccg cgaagaagtc agccagcgat gccagcacat cagcccgtga
     4801 ggcggcaacc catgcgactg atgctgcagg ctcagcacgc gcagccagca cgtcagccgg
     4861 acaggccgcg tcgtcggctc agtcagcgtc ttccagcgca ggaacggcat caacaaaggc
     4921 cactgaagca ttaaaaagtg ctgccgctgc agagtcctca aaaagcgcgg cggccaccag
     4981 tgccggtgcg gcaaaaacgt cagaaacgaa tgcggcagcg tcacaacaat cagccgccac
     5041 ttctgcatcc accgcgacca cgaaagcgtc agaagctgcc tcctcagcca gggatgcgtc
     5101 ggcttcaaaa gaggcggcaa aatcatcaga aacgagcgca gcctcgagcg ccagtagttc
     5161 cgcttcctcg gcaacggcgg caggcaattc cgcgaaagcg gcaaaaacgt ccgagacgaa
     5221 cgctaagtct tctgaaacgg cagcggaaca gagtgcctcc gcagcagcag actcaaaaac
     5281 agcggctgca tcatctgcca gcgccgcgtc aacaagtgcc gggcaggcct cagccagtgc
     5341 caccgctgcc ggaaaatcgg cagaaagtgc cgcatcgtct gcttcaacag ccacaacgaa
     5401 ggctggcgaa gccactgaac aggccagcgc agcagcgagt tctgcttccg cagcgaagac
     5461 atccgaaacg aacgcgaaag cgtcggaaac cagcgcagaa tcctcaaaaa cggctgccgc
     5521 atcgtcagcc agttcggcgg cgtcatcggc atcatctgcg tctgcttcaa aagatgaggc
     5581 gaccagacag gcgtcagcag cgcggggcag cgccacgacg gcatccacga aggcgacaga
     5641 ggctgctggc agtgcgacgg cggcagcaca gagcaaaagt acggcagaat ccgcggcaac
     5701 gcgcgccgag acagcggcaa aacgggcaga ggatattgca tccgctgtgg cgcttgagga
     5761 tgcgagcacg acgaaaaagg ggatagtaca gctcagcagt gcgaccaaca gcacttccga
     5821 gtcacaggcg gcaacgccaa aagccgttaa ggccgcgtat gacctggcta acgggaaata
     5881 caccgcacag gatgcaacga cagcacagaa agggatagtc cagctaagca gcgcgactaa
     5941 cagcacttcc gagtcacagg cggcaacgcc aaaagccgtt aaggccgcgt atgacctggc
     6001 taacgggaaa tataccgctc aggacgctac gacagcacaa aaaggaattg tccagctcag
     6061 tagtgcaacc aacagcgcat ctgaaacgct tgctgcgaca ccgaaagcag tgaaagcagc
     6121 taatgacaat gcgaatggtc gggtaccttc tgcccgtaag gtgaatggta aggcgctttc
     6181 agcggatata acactgacgc cgaaagatat tggtacgctt aactcaacaa ctatgtcatt
     6241 cagcggtggt gctggttggt tcaaattagc aacggtaacc atgccgcagg cgagttctgt
     6301 tgtttcaatt acgttgattg gtggggctgg atataacgtc ggttcacctc aacaggcagg
     6361 tatatctgaa cttgttttgc gtgcaggtaa tggtaatccg aaggggatta ctggtgcttt
     6421 atggcagcgc acatcggcag ggtttacaaa ttttgcctgg gtcaatacat ctggtgatac
     6481 ttacgatatt tacgttgcaa tcggaaatta tgcgactggt gtaaatattc aatgggatta
     6541 taccagtaat gccagcgtga cgattcatac gtcaccagca tattctgcta ataagccgga
     6601 agggttaacg gacggtacag tttattcact ctatacgcca tcagagcagt tttatccgcc
     6661 tggcgcacca atcccgtggc catcggatac cgttccgtct ggttatgccc tgatgcaggg
     6721 gcagactttt gacaaatctg cctacccgaa acttgcagtc gcttatccgt caggcgtgat
     6781 ccctgatatg cgtggctgga cgattaaggg caagcccgcc agtggtcgta ccgtattgtc
     6841 tcaggaacag gacggcatta aatcgcacac ccacagcgcc agcgcatcca gtacggattt
     6901 ggg
//
LOCUS       DAZPEP010000195         6835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZPEP010000195 DAZPEP010000000
VERSION     DAZPEP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.196"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(284..769)
                     /gene="sixA"
                     /locus_tag="ACHUT9_003868"
     CDS             complement(284..769)
                     /gene="sixA"
                     /locus_tag="ACHUT9_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFR1350238.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(972..3116)
                     /gene="fadJ"
                     /locus_tag="ACHUT9_003869"
     CDS             complement(972..3116)
                     /gene="fadJ"
                     /locus_tag="ACHUT9_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFR1350239.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3116..4426)
                     /gene="fadI"
                     /locus_tag="ACHUT9_003870"
     CDS             complement(3116..4426)
                     /gene="fadI"
                     /locus_tag="ACHUT9_003870"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFR1350240.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4606..4890)
                     /locus_tag="ACHUT9_003871"
     CDS             complement(4606..4890)
                     /locus_tag="ACHUT9_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFR1350241.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5262..6602
                     /gene="fadL"
                     /locus_tag="ACHUT9_003872"
     CDS             5262..6602
                     /gene="fadL"
                     /locus_tag="ACHUT9_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFR1350242.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYISWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1702 a         1828 c         1624 g         1681 t
ORIGIN      
        1 ttaatcaagt ttcaattaca tcctgcctga ttattcaact ctattttctc tattatctgt
       61 attaataaat gacaatgagt gtcaaacaga cattattcat ttcaacatgt aactttgggc
      121 aaaatcaaga tacgcgcaga agaataattt ccgaatgaaa gtagaaagtg ttttatttat
      181 agatggggag agtcttcgat agtagtgaga ttgacagata tccgctgcct gatgagcaat
      241 tatgcccaat gaatctacct cattgggcat aatttggaac tcatcagata gcttttgcca
      301 tcttcagatt acacgggctc atctgccagt tgaatgtgcc gtttccgctt tcatcaagcg
      361 ttacgctggc aatggccgag gtggtaaaca taggcggcgt ttcgcccgga cataactcgg
      421 caaccagata cccgactaac ggtaagtgag agatcaccag taccgaagca acaccttcat
      481 tggtcaacgc ctgcaaatag gcactgacca gaccaacatc gccgcagggc gttaactccg
      541 gcagaacttc tgcactggaa ggcaggttca gacaatcccc tacttcttcc agtgtttgct
      601 cggctcgcag gaacgggctc accagaacgc gttcgatttc cactttttga cctttcagcc
      661 agttcgccat caggcgagat tcgtcacaac cattagtggt cagaggacga accgaatcac
      721 tggcggcatc aagggctgcg tcgccgtgac gcatgataaa aacttgcata ttgcaccgct
      781 tttgttaacc agattcgtcc gttttctttc atctgaaacc agagaaagaa aacggtggcc
      841 ggcattgtgc cttatccatt caccgaatga aacgctgttt tttacctcaa tggcgtaagt
      901 atagtcaata tgcgtttaca ttttgaccaa cactccgcca ttcagcgcgg attcatatag
      961 ctttgacctt cttattgaag gtcagttgca gttgttttcc aaaaactttc cccacgcgcg
     1021 cccatctcga ccaaacgctc gcaaggggta aaacgggaac catactgcgt ggccagtcgt
     1081 tgcattattg caaccacttc gcccgcgccg agagaatcga tatagcggaa cggtccaccg
     1141 agaaatggcg gaaaaccaat gccaaatacc gcgccaatat ccccgtcacg cacgctacgg
     1201 ataacctgct catcaacaca acgtactgct tcattcagca tcaacatcac acaccgttca
     1261 gcaacctgcg gtgcggagag tcgcccctgc ccttgtgcgc caatgagcgg gtaaatggcg
     1321 ggatcgacct gttttttgct tttacgccct ttctgaccat aaagatagaa accccgacca
     1381 ttttttctgc ctttgcgatc gtcgttcaaa attgaagaaa caacatttgc aggcgcgcta
     1441 aaacgttctc cataagcggc ttccagtaca ggaataattt tagtcccggt gtcgattcct
     1501 acctcatcca aaagttggat tgggcctacc ggaaaaccaa atttcactag cgcggcatca
     1561 atgtgctcta cccgttcacc ttcggtcagc atgcggatag cttcattaat gtaaggcgct
     1621 aagatgcgat tgacgtaaaa cccggctttg tcacgcacga caatgggcgt tttgccctgt
     1681 tttttcgcca gttttaccgt agtggcgatg gtttgcgccg atgtacccgc atgaggaata
     1741 atctctacca gcggcatttt ttccacagga ctgaagaaat gcaggccaat aacttgctca
     1801 ggtcgcgcgg cgtgagcggc gatatcacca atcggtaaag atgacgtgtt cgaagcaaaa
     1861 atggtatgag cggcgcaatt ttgctccact tccgccacca tctgttgttt taattcgaga
     1921 ttttcaaaca ccgcttcaat aatcagatcg cgatgggcaa agccgcgata gtccgtcgtt
     1981 ccggagatta atgccagctg tttgtcacgt tcgctggctt tgagatgacg acggcgaact
     2041 ttgccctcca gctgatccca actgtacttc agcgcatgat ttatgccctg cgggttgata
     2101 tctttaattc tgaccggaag ccccgcttta caagcagtga cataagcaat accgccgccc
     2161 atcaagccac cacctaaaat tcccacgctg tttaatggcg caggcggtgc atcactaccg
     2221 ggatctttct tcacgtccgt actggcaaaa aagatactac gcaaagcttg cgattgcggc
     2281 gtcatcgcca gttcgccaaa ggcgcgagct tcagcgtcat agccgctgct ggtgccctgc
     2341 gctaatcccg tttcaacaac ctccaggatg cgttctgtcg ccggataatt gccttgagtt
     2401 ttgtgttctg ttttcttgcc gaccattttg aacagcagcg cacgacctaa cggccccgcc
     2461 agaatacgct cgcgtacagg tagagggcgg gaagatgggc gatccttctt cgccagctca
     2521 acagcggctt ccagcaaaat ggagtgcgga acaacgtcat ccaccagccc cagctttaat
     2581 gcctgtttcg cccgaagttg ttttccggtg agaatcatct ctaatgctgt actgacgcct
     2641 atcagacgcg gtaaacgctg ggtgccgcct gaaccgggta acaatccaag ttgtacttca
     2701 ggcaaaccga gcaccgtttt aggatcgtca gtacaaacgc gaccgtggca cgccagcgcc
     2761 agctccagcc caccgcccag gcaagcgcca tgaatagccg cgataaccgg aatgggcaaa
     2821 gcatgaatct ccgccatcaa ctgttggccc tgccgcgcca gtgcttccgc ttcttgcgcc
     2881 gttttgcagt tgccaatcat gttgatgtct gctccggcga taaaattgtc cggtttcgcg
     2941 gagataaaca ccacgccgcg caactctttg ttttcacgga gttgcttaat aatggcgcgc
     3001 acctgcgagg caaattccgc cttcagggta ttcattttct catccggtac atcgatggtg
     3061 atgatagcaa tgttgtccag acgaacatta agagtaaacg ctgatgccat ttccattatt
     3121 ccgcctccag aaccattgcc gcgccaagcc caccggcagc acaggcggta actaaaccaa
     3181 atccaccgcc gcgacggcga agttcatgca atgtctgggt aatcatccgc gcgccggtcg
     3241 ccgcgaaggg atgcccgtaa gcaatcgaac cgccaagcac gttaaatttg ctatcgtcca
     3301 cttcgccagt ggcatgtgca cgccccagta cttcacgagc aaaacgttca ctacccagca
     3361 actgaatatt cgccagtgtc tgagctgcaa aggcttcgtg catatcgatc aatgtcagtt
     3421 cgctcatcgt caaaccggca cgttccagcg ccagcggtgt tgaccaggct ggaccgagca
     3481 acatgtcctg ccagacatca atcgcagtaa atgcgtagct gcgcagatac cccagcggca
     3541 ccagccctaa ttctttcgcc cgggattcag tcatcaggat caccgctgcc gcgccatcgg
     3601 tcagcggcgt actgtttgcc gccgttaccg ttccgtgttt gcgatcaaac gccgggcgca
     3661 gctttgcgta atcggcaagc gaggaattac cgcgaatatt gttgtcttcg acaagcggtt
     3721 gtttataagg agggataaag gcagtcatca cctcttcttt gagttttccg tctgaccatg
     3781 cctgagcggc acgctgatgc gaacggtgcg ctaatgcatc ttgctgttct cgggtgatgc
     3841 cgtaggtttt cgccatttgt tcggcggtgt cgcccatccg caagccagta gaatattctg
     3901 ctaccgcagg cggtacgggc attaagtcgc gcaaacgcag gcgagagaag agtttcaggc
     3961 gctggctcat ggttcgagct ttgttgacat caaccagcac gcgcgccagt tttttactga
     4021 cgccaattgg caatacggaa gaggaatctg ccccaccggc aatccccgct cggatagttc
     4081 ccgccatcag gctttctgcg acgtttgcaa ccgcctggaa actggtagcg caagcgcggc
     4141 tgacgctgta agcatcggta tgcacattca ttccggtacc gagaacaatt tcacgcgcaa
     4201 tgttgggggc ttcaggcatt tgtacgacct gaccaaagac cagctgttca atcacttcag
     4261 cggggatctc gctgcgtgcc agcagttcgc ctaccaccat cttccctaaa tcaaccgcgg
     4321 gaatgccatg aaaagccgtc gcctggcggg caaaaggcgt acgtaaaccg ctaacaatgg
     4381 cgatacgatc gccctggcgg gtaaccagcg gtaaaacctg acccataaca ctcccctgta
     4441 aaaaaataaa taaagtggtc tgacctgatc atagtcttaa ctattttttt acatttagcc
     4501 aagcggagaa aaaggaaagt gggagctatg acacagtgaa gacaggagaa agaaaagaaa
     4561 acgcccctgc cagcaactga caggggcgaa cgtacccgag aggaattaac gcagacccag
     4621 ctggaagatc agcatttcag cttcgcaggc aaaagtgaaa tcgatatcca ggcgcacacc
     4681 gtcagactct tcagtgaaag tcggggagat tttgcacggt tcggattcca cgctacgggc
     4741 tttttcagtc agcgccgcca gcgtttgttc tgcttctgcg cggtttgcaa acacgcggct
     4801 gtaagacgcg gtgcagtcgg agttgtccat aatggtgcca acatccatac agcagcaaac
     4861 cggggtttca tcagcactac atttactcat cgttgatttc ctctgtatgt gcacccaagg
     4921 tgccagataa acgttgtgga tattttacgc ttccggaaag tgctgctcca gttgttaatt
     4981 ctgcaaaatc ggataagtga ccgaaatcac acttaaaaat gatctaaaac aaaattcacc
     5041 cgaatccgcg agtgcgcaac ctccaaattt tgccagctgg atcgcgtttc ttagatcata
     5101 tttgaaaaaa gatggaaaca tacttgcagc attccagctg gtccgaccta tactctcgcc
     5161 actggtctga tttctaagat gtacctcaga ccctacactt cgcgctcctg ttacagcacg
     5221 taacatagtt tgtataaaaa taaatcattg aggttatggt catgagccag aaaaccctgt
     5281 ttacaaagtc tgctctcgca gtcgcagtgg cacttatctc cacccaggcc tggtcggcag
     5341 gctttcagtt aaacgaattt tcttcctctg gcctgggccg ggcttattca ggggaaggcg
     5401 caattgccga tgatgcaggt aacgttagcc gtaaccccgc attgattacc atgtttgacc
     5461 gcccgacatt ttctgcgggt gcggtttata ttgacccgga tgtgaatatc agcggaacgt
     5521 ctccgtctgg tcgtagcctg aaagccgata acatcgcgcc tacggcatgg gttccgaaca
     5581 tgcactttgt tgcaccgatt aacgaccaat ttggttgggg cgcttctatt acctctaact
     5641 atggtctggc aacagagttt aatgatactt atgcaggcgg ctctgtcggg ggtacaactg
     5701 accttgaaac catgaacctg aacttaagcg gtgcgtatcg cttaaataat gcatggagct
     5761 ttggtcttgg tttcaacgcc gtctacgctc gcgcgaaaat tgaacgtttc gcaggcgatc
     5821 tggggcagtt ggttgctggt cagattatgc aatctcctgc cgggcaaact cctcaagggc
     5881 aagcattggc agctaccgcc aacggtatcg acagtaatac caaaatcgct catctgaacg
     5941 gtaaccagtg gggctttggc tggaacgccg gaatcctgta tgaactggat aaaaataacc
     6001 gctatgcact gacctaccgt tctgaagtga aaattgactt caaaggtaac tacagcagcg
     6061 atcttaatcg tgcgtttaat aactacggtt tgccaattcc taccgcgaca ggtggcgcaa
     6121 cgcaatcggg ttatctgacg ctgaacctgc ctgaaatgtg ggaagtgtca ggttataacc
     6181 gtgttgatcc acagtgggcg attcactata gcctggctta catcagctgg agtcagttcc
     6241 agcagctgaa agcgacctca accagtggcg acacgctgtt ccagaaacat gaaggcttta
     6301 aagatgctta ccgcatcgcg ttgggtacca cttattacta cgatgataac tggaccttcc
     6361 gtaccggtat cgcctttgat gacagcccag ttccggctca gaatcgttct atctccattc
     6421 cggaccagga ccgtttctgg ctgagtgcag gtacgactta cgcatttaat aaagatgctt
     6481 cagtcgacgt tggtgtttct tatatgcacg gtcagagcgt gaaaattaac gaaggcccat
     6541 accagttcga gtctgaaggt aaagcctggc tgttcggtac taactttaac tacgcgttct
     6601 gataacgcgt tcgcctggat aaagtcacct gcatagcagg tgactttaac tcccccactt
     6661 caccgaaagt agtgtcctca ttgcttacct cctgagtttt gcaaacagcc tgttggcagc
     6721 ttgccctttc aaatcaataa gcggtatcca cagacaaacc tgaagggaaa ggcatatttt
     6781 caggcgttct gctcgtcctt ctcaaagagt ttacttttct gcatttccag gatac
//
LOCUS       DAZPEP010000196         6826 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZPEP010000196 DAZPEP010000000
VERSION     DAZPEP010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.197"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(155..667)
                     /locus_tag="ACHUT9_003873"
     CDS             complement(155..667)
                     /locus_tag="ACHUT9_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFR1350243.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            862..1626
                     /locus_tag="ACHUT9_003874"
     CDS             862..1626
                     /locus_tag="ACHUT9_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350244.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1577..2980
                     /locus_tag="ACHUT9_003875"
     CDS             1577..2980
                     /locus_tag="ACHUT9_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HFR1350245.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            2980..4446
                     /locus_tag="ACHUT9_003876"
                     /pseudo
     CDS             2980..4446
                     /locus_tag="ACHUT9_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            4337..5080
                     /locus_tag="ACHUT9_003877"
     CDS             4337..5080
                     /locus_tag="ACHUT9_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFR1350246.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5084..6304
                     /locus_tag="ACHUT9_003878"
     CDS             5084..6304
                     /locus_tag="ACHUT9_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFR1350247.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            6308..6814
                     /locus_tag="ACHUT9_003879"
     CDS             6308..6814
                     /locus_tag="ACHUT9_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350248.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         1816 a         1622 c         1868 g         1520 t
ORIGIN      
        1 atcagcgacc agacagcatt gagaatgctt caggagtaca tcaggatcca atgccttggg
       61 gggtagcggt aattttactc attatccttc aaatcagatt ctgttgtcag aggaatgggg
      121 gaagctggat tcgcaagagc tttttatggg cagtttagct aaaaaaaggc aacacgaagc
      181 taaaactacc aacataaagt gctgaccagc tagcagctga aatcagactc actgtataca
      241 ctttcactgg atgaagttgc aacattcctg ccaccaaagg agcaatataa cgcagcacgg
      301 cgataaagcg cgaagtgaaa agtattgaca cagagttatt ttgcaactga aggcgaaccc
      361 gttctattgt attggaacgg cttgatatta tacatgcaag ttgagggata tgcccaatta
      421 ttataccaag atggtaatta acgatagtac cgcaccatgc gccactcatg accgtgattc
      481 cagcttccca tggtgaaaga gtcgtcttgc tgactgtaat tacagtggcc atcataattg
      541 atgctggtgg caatatcgcc gaaatgagta aagtggattt tgtgaacgct attataaaaa
      601 gaagccccca taggcgatat ggatgaagta caaaatagtt catcaaagca tttatccact
      661 ccatcaatgt ctttccttct gtgaattttt ctttgctatt taaccgtgat acgacttaat
      721 ttaggcttaa acaccttaca tttacaaggt actcctgtgg ggggccctgc cacggagcgt
      781 cgcaggagtg agtgatgagg aagaattaaa gcaacctatt gtgacaggca gcgataacag
      841 taaatcacag gaagcataat catggcaaaa ccggactggg aggccatcga atcggcatac
      901 cgggccggag tccttagcct ccgtgatata ggcgagaaat acggcgttac agaaggggct
      961 atcaggaaga gggccaaaaa gcttggttgg gcacgcagtg gcggtacgca ggtttgcaaa
     1021 aatggtacgc aaaaaaggaa agtgcgtacc agcagaaagc ctgccattac tggccttaca
     1081 caaaaaagta cgcaactaaa aacagaatct acaccggata cgaaaccgat acgcggaatg
     1141 cgtaccgatc ccccgactaa cccattccaa cccggtaacc agcaggcatt aaaacacggt
     1201 ggttatgccc gtcgccttct gctcaaagat gaggtgatag aggacgctaa agcgttgacg
     1261 ctcgaggacg aattatttcg ccttcgtgct aacaaccttg tcgctgcaga gaatattggt
     1321 cggtggttgg tgtcgctgga agatgctaat ggggaccagg aaaggaagat gctgattgaa
     1381 aatatcagcg ccgccgagaa agcaatgatg cgcaatacag ttcgtattga gtccatcgtt
     1441 ggcacgcttg cgacggtagg aaaaatattt gctgatacag cctaccgcaa ggccgccact
     1501 gataaggtgt ctctggaggc tgatcgtctt cgccgtgatg caggtattga tgatggcaat
     1561 ggagagcgtg acctcaatga cttctactct gacatccaaa ccgacgctga atccggcttt
     1621 acgtagtttc tggactatgc gggcacgtaa caaagtgctt tatggtggtc ggtcatcgtc
     1681 aaaatcatgg gatgccgctg gcattgccat atttctgtcg aataaataca ccctgcgttt
     1741 ttgttgtgcc cgtcagatcc agaataaaat cgaagagtcg gtgtataccc tgctcaaaat
     1801 tcagatagac aggtttggtc tgcggcaccg tttccgtatt ctgaacaaca aaatcattaa
     1861 ccgggttact ggctcggaat ttgtttttta tggattatgg cgcaacatcg aagaaattaa
     1921 gtcactggag gggatcgatg tgttgtggct ggaagaagcc cacgcactga cggaatacca
     1981 gtggaaaatt ctggagccaa cgatccgtaa agagggttcg gaatgctggt tcatattcaa
     2041 ccccggactt gttactgatt tcgtctggcg caacttcgtt gttgatccgc ccgaaggcac
     2101 tctcatccgc aaaattaact atgacgaaaa tccgtttctg tctgacacca tgcttaaggt
     2161 tatcgacgcg gcgcgacgcc gtgatccgga tggttttaaa catgtgtatg agggcgttcc
     2221 ggagtctgat gatgatgcgg caatcatcaa actgtcttgg atagaagccg cagtggatgc
     2281 gcacaaaacg ttaaatttcg aacccagtgg aagaaagcgt attggctttg acgtggctga
     2341 cagtggtaca gataagtgcg ctaacgttta ccgtcacgga tccgttgttt tctgggccga
     2401 cgaatggaag gccaaagaag atgaattact gaagagctgc cagcgtactt atcaggcggc
     2461 gctggagcgt gaagcagata ttgtttacga ctctatcggt gttggtgcgt ctgccggtgc
     2521 taaattctct gaaattaacg ctgaccggaa gagcgagaac gcatacgcgc gacgtgtgaa
     2581 ttaccagagg tttaacgccg gtgctggtgt gcatgagcca gatgacgagt acaacggcat
     2641 ccccaacaaa gactttttcg caaatcttaa ggctcaggca tggtggctgg tggctgaccg
     2701 tttcagaaat acgtttaacg ccattaacaa cggagaacag tatcctgtgg atgagctgat
     2761 cagcatagat tctcgttgtc cgttgcttga aaagctgaaa ctggaactga caacacctca
     2821 tcgtgatttc gaccgtaacg gacgtgtgat ggtcgaaagt aaaaaagacc tcgcaaaacg
     2881 cgagataccg tcaccaaacg ttgctgatgc attcattatg gccttcgcgc caatcgatac
     2941 atcgctggat atctgggaac agctggggag acaggcctga tggcacgaaa caaacaagcc
     3001 ctgcggcgaa ctgtgcaggc cacagctgat ggttatgaga attttattgc ccgcgtaggg
     3061 atgcagacac ctaaccagca ctcagcatcc acctaccggg ctaatttcac cagtcgtaac
     3121 cgcatgctgg tggaatggtc ctatcgttca tcctagatca tcggcgaagc agtcgatgct
     3181 atcccggatg atatgacccg caaaggcatt cgcatcactt cggaaattga tgcaaaagat
     3241 cgtggcattc tcgaatcaca actggatgag ttgcaaatct gggatgcgct gaatgacgtg
     3301 ctgaaatggt cgcgcctcta cggcggcgcg gtgggtttca tcatgattga ggggtaggca
     3361 ccaatgaccc cgctgcgacc cgaaaccatc ggtaagggca agtttaaggg gattctcccg
     3421 ctcgaccgct ggatggtcga cccggcactg acccgccgca ttaaagatat ggggccggac
     3481 ctgggtaaac ctgagtttta cgatgtggtg accacagcaa cgggaattcc tgcctggcgc
     3541 attcatcaca gtcgcctgat tcgctttgat ggcgtcacgt tgccatttca gcagaagatg
     3601 accgagaacg aatggggaat gtcggttgta gagcgtatct gggatcgtct taccgcgttc
     3661 gacagcgcta ctgtcggcgc ggcgcagctg gtctacaagg cgcatttgcg tacctacagc
     3721 gtggagaagc tacgcgagct tatcgcactt ggtggtcctg cgtatgaagc gttgctgaag
     3781 aatatcgacc tgattcgaca gttccagagt aatgaaggca tgacgctcat ggactcgcgg
     3841 gataagtttg aaacccatca gtacagcttc agtggtctgg atgacatcct ttcgcagttt
     3901 gtagaacaga ttagtggcgc tgttggtatc ccactggtgc ggttgttcgg acagtccccg
     3961 aaaggatttt ctaccggtga tgcagacctt gccaactatt acgatcgcat cagttcgttg
     4021 caggagaggc gtttacgtct tccggtgcgg cggatactgg acatcatgca tcgttcggaa
     4081 cttggcaagc cgctgccgga cgatttcacg tttgagttta acccgctctg gcaaatgtct
     4141 gatgtcgatc gctcaacggt ggcgttaaac accaccaacg caatcagtac ggcgctgggt
     4201 gatggtctga tgacactgaa agccgctatg actgatttgc gagaaaattc tgacgtaacc
     4261 ggcatcgggg catccattac cgacgaggac atcgagaatg ccgaagatga agcgccgccc
     4321 ggcatcggcg aacctgatga cgaaccgcag gagccgtcag gcggaaatcc ggtatcgaac
     4381 cagcctacgc aggatagcga gggcggtcgg agacatcgta aatggtcgct acgatggttc
     4441 aaatgacagt atcacggaaa ttattgcggc gctggagcgt tacagtgaaa tcatcacccc
     4501 ctgggcgaca aaggtcgcgg aaaactttac tgcggaccta acccggcaga acgagaaagt
     4561 ttggcggcaa cacagcaaga acatcagtcg cgagctccgc aatcttgtgg aaagcgctcc
     4621 tgtgggccag gtgatgcaat ccatcatcgc cgaacaggtc aagtacatca aatctctgcc
     4681 tctcgaggcc gcagacaggg tgtacgacat ccagaacaaa gcgatagagg ctgttgtcac
     4741 cggtggtcgg gcggagcagt ttgctaaaga gattgcatct accggcgatg ttgctaagtc
     4801 tagggccgat ctgattgccc gaacggaact gggaagagca acgggcgcgc tggatatggc
     4861 ccgagcgata gctattggct cggatggtta tatttggcgt acagccgatg atggcgatgt
     4921 ccgagattcc cacgatcaca tgaaaggtaa attcgtccgc tgggattcac ctccaacttt
     4981 ggacggcatg accggccacg cgggcgagct gccaaactgc cgctgctata aagagatcgt
     5041 gtttgttcgc gttccattcg caatgaaaag ggcagcataa cccatgaaat acttttttga
     5101 gaccaggctc ggggaaaccc gataccgcct ggctgacggc tcgttgctgt gcaaagacgt
     5161 gccgatagga cgaacaggta agcagctcta tggtgctgat gacctgccaa aactgaaacc
     5221 cgataagttc ggtgaaatag tcgtcacgcg ttctcctgag caggtattcc atccggccac
     5281 gcttgcctca ttcgaaggga tgagcatcac gatcctgcat cctgaagatg aaaacgggaa
     5341 tgtgcggctg gtaaatcccg agaactggaa agagcttgct gtcgggcacc tccagaatgt
     5401 ccggcgcggg acgggtgagc agtctgattt gatgctggct gaccttatcg tcaaagacga
     5461 aagcgccatt cagcttatcg aagatggcct gcgcgaagtg tcgtgcggct atgacgcgga
     5521 gtacgagcag accgagccag gtaaagccga gcaggtcgat attaccggaa accatgtggc
     5581 tcttgtccct aaaggcagag ccggaaatcg ttgtgcaatt ggagacagag acacaatggc
     5641 aaatcaaaag aaaaactggt ggaaccgcat gcgtgcggcc atcaagacag gagatgccga
     5701 caccatgaac gaactggtgg agtcggctcc cgcatcggtt acaggagatg agggggattt
     5761 gccgcagggc gttaatctca acatcaacct gtccccgcag caaccactac cggacaaagc
     5821 accagagatg ggcggagatc caaccggcga cagtgatgat gacctcaaaa cattactgaa
     5881 agccctgctg gctaagctgg aaagaaatgc cacgggcgat aacgataata agcctgacga
     5941 taatccgacc ggtgacggcg aggacgatga agaggaaacc acgattactg gtgactcagc
     6001 ctggcgtgcc gaagttatcg ttccgggtat cgatctgagc cgtaagatga aaccgaccgc
     6061 gttcaaacgc gaggttctgg cttctgctga caaaacgctg gttcgccaga tagtcggtga
     6121 tgcggatatc cgcaaattac cgaaacaatc ggtcgacatg gcgtttaatg ccgtgtctga
     6181 ggttgccaaa gggcgaaaca cccgcgccac caccggcgat gcacagcgcc taaacatggg
     6241 catgaccagt atcgcttccc tgaacaaaca aaacgctgaa ttctgggcaa accgtaaagg
     6301 gtaaaaaatg aataatgtat ttctgtaccg gatgcctgtt ggcattgccg gggctgtctc
     6361 tcgcccgcag gacttaaccg tcgaaccggt ggtccttaaa tccgataacg ccttcgctgc
     6421 ctatggcctg gctggtaaat acgatgatga cggttttttc gtgccgctgg cagatggtga
     6481 taccgcagac aaggtgaagg ggatctacgt gcgcccttat ccgaccacgt cgcagccgga
     6541 catggttcgc caggtgggaa caggcaagaa cttcccgggc gacgccatga agcgtggcta
     6601 cgtgaccgtt aatctcggtt ctgattttga tgccagcact atcaaaaaag gcgatccggt
     6661 atacgttgtc gtctccactg atgaatccat caaagtgccg ctgggtggat tcatgtccac
     6721 gtcagtcagt ggcaaaaacg tggtgctgac caacgctgaa ttcacaggtg ccggtgatgc
     6781 tgacggcaat gcagaaattt cctggaagat ttaaggaaca gacgaa
//
LOCUS       DAZPEP010000197         6824 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZPEP010000197 DAZPEP010000000
VERSION     DAZPEP010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.198"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHUT9_003880"
     CDS             complement(<1..214)
                     /gene="cas6e"
                     /locus_tag="ACHUT9_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFR1350249.1"
                     /translation="MAVWGTHRQSGAVAQQGGTMYLSRITLHTGQLSPAQLLHLVDRG
                     EYVMHQWLWDLFPGGKERQFLYRREEL"
     gene            complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHUT9_003881"
     CDS             complement(139..885)
                     /gene="cas5e"
                     /locus_tag="ACHUT9_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFR1350250.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHUT9_003882"
     CDS             complement(896..1951)
                     /gene="cas7e"
                     /locus_tag="ACHUT9_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFR1350251.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHUT9_003883"
                     /gene_synonym="cse2"
     CDS             complement(1963..2499)
                     /gene="casB"
                     /locus_tag="ACHUT9_003883"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFR1350252.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHUT9_003884"
                     /gene_synonym="cse1"
     CDS             complement(2496..4058)
                     /gene="casA"
                     /locus_tag="ACHUT9_003884"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFR1350253.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFI
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRISELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVLLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLNKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERIMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(4156..>6824)
                     /gene="cas3"
                     /locus_tag="ACHUT9_003885"
     CDS             complement(4156..>6824)
                     /gene="cas3"
                     /locus_tag="ACHUT9_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFR1350254.1"
                     /translation="KNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKR
                     NCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKN
                     YEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGRPPDRMD
                     ELDNFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADW
                     TGSSTRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIENPT
                     PLQQKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMAT
                     ANAMYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFS
                     QGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACD
                     AYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDY
                     PWLTHVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSV
                     DDAIKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDSSQRAGKVLI
                     CTQVLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELL
                     ILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESV
                     YGEDVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAE
                     ESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIE
                     QRQDPEMANVILVNDDESCGYSATEGLIGKVG"
BASE COUNT         1649 a         1857 c         1620 g         1698 t
ORIGIN      
        1 ggagttcttc ccgacgataa agaaattgcc tttctttgcc gccagggaaa agatcccaca
       61 gccactgatg catcacatat tcaccgcgat ccaccagatg cagcaactgc gcaggcgaaa
      121 gctggccggt atgtaaggtt attcttgaga ggtacatggt tcctccttgc tgagccacgg
      181 cccctgattg acggtgcgtt ccccaaacag ccactgctga cgatttaaag gaacatcccg
      241 gcggcgtaat attttgctcg cgaccaggcc gtcgtgtttc ccttcccacc agcattcatc
      301 ctgaagtttc gggagtgaga ctttcagttt atggaaatga tcctgatact gttggtatgc
      361 attacataag gcatcacacg cgttgccttc gagcagtaac ggcgcaagag gtaatgccag
      421 cggatgactt tttcgcccca gataaagcgg aaaaactgga tggcgtaaac cgtcctgcaa
      481 ctgttcgagg ctgtaaggcg catcggctgt tgttgccacc gccaccatcc accaggcatc
      541 ggtgtagtag tcgcgccggg agataatcgc gctcaaaagc tcagggtcgc tcaactcttc
      601 acgacggctg aaataacgtg ctttacgcac ctcttttggc atctggaccg tgtgataatc
      661 ccgtgcccag cgcgggttac ggctggcgca aaccaccagt gaatagtggc gattaaacgc
      721 gtttaagcgt tcggtatcat cacgccgaat ccctaccccg gcagccagta gccccagcaa
      781 tgctgagcgc gaaggaagtt catgggtatg acgaacttca cctggagcat cgacacccca
      841 ggacgccatc ggcccatgaa gctgaaaaat cagatattgg ctcataagcc gccccttacg
      901 cgcagataaa gtccagcacg tccttcatgc ttccctgttt gttcatcacg tcaaaacttg
      961 cgcattcggt cttctgttcg tagaccgtat tcatgttttc gcgaagcgtt gtaatacgct
     1021 gcaccgccac atctaactga cgtgtgccat taatgggttc atagaaagcc gccgccagag
     1081 agcgtggttg atcggtgcct ttttctgcca gcgcccagga agcataggca cggctggcaa
     1141 agctattttg tttaccggtt ggggaaactt taagcgcggc ttccgtaaaa gcgcgtatgg
     1201 tctgattagc taacgcttcg tcaccgccga ggttttctac cagcagatct ttatcaatgc
     1261 agatataggt gtagaaaagt gctgaaccga atccggtttc tccgagatgc ccggcaccgg
     1321 catcttcaga ggcctggcgc aaatcatcaa cggcggtgaa aaaatcatct tcgacaatcg
     1381 tttcactgac accaaacgca tgcgcgacct ggcaggcggc ttcaacatta aactcaggtt
     1441 tattcgccag catacgacca aacatagcga tatctaccgc tatacggtct ttacgtaaca
     1501 acgcgagatc ttcctctttt ggcgcgcgct tttcttctgc cagttgatga gcaagcgctt
     1561 ttacggcgtc aaattctgcc gggctgatat gaactaattg ttcagtttcg gcgttagtga
     1621 gcggatcttt tggttttttg tcatttttag ctttcccaag ataatcagca atttttgccg
     1681 cccattcgat ggctttttta tcttcaatcc ctttctcaat caggatagtt gccgcctcac
     1741 gcgcaatacg cccactgcga ataccaatat ggcccgccag tgcctgttca aaaagtgcag
     1801 aagtgcgcca cgcacgtttc agactttgcg aggaaacgcg cagacgcgtt gctccaccca
     1861 gcaccacagt ttttggcgca ccggtgtcat cacggttaag gttggcagca gggtaagcgg
     1921 ttaacacatg aagctgaata aatgttgtca tgagatagtc ctttattgat tttgttcgtt
     1981 atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa attcggttgg
     2041 ccgctgctgg cggcggtgat gattgagcag gtcgtcgctc tcctggcacc agcggaaaat
     2101 gccatctgcc agggagtcaa gattgacgga accgtttaat aatctgacgg cgcggcgtaa
     2161 ctggcgcagt aattcatccg gtgtttttac tgctgacagg cgagtaaaac gcccctttga
     2221 cattaccgcc gccagttgcg cggcaaaagg ctgccgttca tcaatggctt taacattggc
     2281 gctaactgcg gctatcagcg ccagtgcggt gatacgccac tcaggttcat cctgccactt
     2341 tatttgtctg ttcttcagaa acaggcgaaa tccatccgtc agacaaacat cattaaccgt
     2401 cgtactacgc cgcagactgg cacgttcgcc gcgtttctcc tgcaattcct cgtgccattt
     2461 gcgcagcgtg gctttgtgct cctcttttac aatactcatt cagcagcctc ctgctttttc
     2521 tccctggcgg ctttagcact ttgcttctcc gccgatgttg taaaatattt cttgcgcgcg
     2581 gtcatgatgc gttccaaatc aacgggctca taaggattgg tgaatacacg ctcgtcaaaa
     2641 tcctgacgcg cgaataacca aatttcctta ttccatttgc tgagtaattc atccgcatcc
     2701 tgaccttctt caatttggcg cactaacctc aggaagcgat gctgagtttt gttccagaag
     2761 tcgatatcca caaaactaaa atcacccctt gcaccttttg gatcggagaa ccatgcttct
     2821 ttcaatgcac tccgtaacag acttagaatc cgtgaagccg tttgcgcagc cagccgcagt
     2881 ttcggtatct ggccttcttt tttattgagc agcagcggga aatgatgttc gtaccaacag
     2941 cgcgctttca tgttgtcgaa atcatagcca aatccccaca ggcccacttt tgcctgcttg
     3001 agactgctgg cattaaagag tttcaccacc agcgcgggaa gttccgtatt gttttctgac
     3061 ttacccgttt cgataaggcc taaccagtcg cgccagatta aaccgcccgg ttgtggtttg
     3121 accgagtaaa actcgccgcc ctctttaagt agtacacggt aaggcgttaa gggatgctgc
     3181 cacatggcat agttagcgcc gtaatttttg gtcgtcatca ggctcagaag cgcgtcgctc
     3241 tgctcaccgc aaatatcgca gttgccgact gtggtggtat taaaatcaat acgaatacgg
     3301 cgaggcattc cccagtacgc ctggagttta ttgacctgct catcggttac caccgcaccg
     3361 gccagttcgc tgatgcgcgt cgggccaagc caggggaaaa ccagatcgtc aaattttttg
     3421 ggtagcggta aatcggcttc atcctgcggc atgacattga tccacagttt gcgccacaag
     3481 ggagtttgtt gattaccctg atattcctgt aattcaatca gagtggtcat cggcccaccg
     3541 ccgcgtaaac cggtgcggta gcctttgcca cctgacggcg cattcaactg tagggagaac
     3601 agagctaacg cagagcaatg tgggcacagg tattccgtta caccacgctt gataaagtgg
     3661 tctttattaa acttcgttgt ttgagcgccg ggaatctcag gcagtagcga agcgaccgga
     3721 actttatctc cggtgagcgc ctcgaaatcc tgtataaatg aaggggaatc tggcccaaac
     3781 tggaaagcat gttctaatga cagcaatgct tcccgtagct tttcagcttc cagtccgtct
     3841 tcccagatat catcccaacg acgatgatct tttggcgcga agctggtttg tagtagcccc
     3901 agcaaaaact gccataccgc accctggaga tctgcccgcg gcgcagaaat atcgacaaca
     3961 ttttcatccg ccagatcgac tggcgccagt ttgcctgtcg ttccgtcttt aaaacgaacg
     4021 ggcaaccacg gggttgtcag aagtgaaaac gagttcatac gcatcctctc gtaaaaaaga
     4081 ccatcccagg tcggttgtcc atctttccat ccttttcggt gtatcaacta ttcagagagt
     4141 cctgtcgcag acatctcaac caaccttgcc aatcaatcct tccgtagccg aatacccgca
     4201 actttcgtca tcgttgacta aaataacgtt tgccatctcc ggatcttgtc gctgttcaat
     4261 gcaccacagc ctgaacgcct ctccctccag taaagaaaac tcatcccgat gttttttcca
     4321 ccagcttcgg cgcactctga caacgctcat ttcccatgcg tgagcaccgg tggcataagg
     4381 cttcaccaca ccgtcaatac aggtagccag ccacagggaa acagactcct cagccagacg
     4441 tgtcgacagt ttttctggga ggtaatcgtt gatattggcg gcatagccgg gcctgaagtt
     4501 cagcacaaac tttttagcca ttgcgcgatc acagtaatat ttgcccactt gctcctgctc
     4561 gctgcgggca aatccttccg gcattaccac gtcctcaccg tagacggatt caataagaag
     4621 ccgggctgcg tgtggcatct gaatagcacc ttgctcacgc agcacacgct gcgtcagcca
     4681 gattcgccca tgatcgggat agacaaatgc actgttacgc atggcactgc caaaccattc
     4741 gtcgccagga gagtcgtccc agacgggagc cagaatcagc aattcagggg gggaacgctc
     4801 gtcctttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct gtaatcgtcc
     4861 tgctcgctga atcagcaaat caacaggggc caggtcggag atcatttcgt ccaggtcaca
     4921 atcgacactc tgctctaata cctgagtaca aataaggact tttccggcac gctgtgagct
     4981 atcttcttta ccaaagcgtg ccagtgtctc cgtttcaatt cgctggcgat cgctaaaagc
     5041 aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg caagcagctg
     5101 acgatgaacc ttaatagcgt catcgacaga attccggatc caggcgatgc atttcccctg
     5161 acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatgaagcc aacccacgct
     5221 gacgctacgc tcaacgtctt tgcgcgtcgc tacccggtgt gagttcacat cggatttcgt
     5281 gacatgcgtc agccaggggt aatcatcctt ttccaggaac ggagcttctt gcaggccctc
     5341 tgtaccccgc gcaaaggcag cgacgagttt gtcgcgctgc tgttgagata acgtggcaga
     5401 aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcaaccctt caagaatgca
     5461 cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt tactcagccc
     5521 cagaagccgc agattattat gtttgaacgg catcaccgcc atcatcgcct gatccagcgt
     5581 gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc aggccgcaca
     5641 tccctgactg aacgtttgtt catccggttc ttctgacccg actaaatcac cagaccagag
     5701 tgattcatta aagcggtcca ttaatgtgcg ggcactatgt gccagcacca ggctggggcg
     5761 ggattctggc gaatagaaag caagccaggt tttgaccagc cgatcgtaca tggcattggc
     5821 cgttgccatt gttggcagac caaaaaacag cccctgagcc ttccccgcag ctatcaacct
     5881 gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt ccagaataaa
     5941 taactgcggt ccttgctggc tgatatccag atccagcacc ttttgctgta acggtgtcgg
     6001 attctcaata aaaggaaaca gcgtattaat tccgttgaaa ggcgcgactt ttgcttttag
     6061 aggaaagacg gttaaggcgt tttgagcctg aactaaagtt ttctgccagt aacctttaat
     6121 atccattggg tgtgcgacgc gtggaaaaaa tcgcgttgac gaacccgtcc agtctgcgag
     6181 cacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc cctcatcatc
     6241 atcccagaat gcggggatct ctatgagcgg aaacagtggt ttgatttcaa ggagaaaatc
     6301 tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac gatcaggggg
     6361 ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcactcgcc taagtttgcg
     6421 cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat gattccacaa
     6481 ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac cttcctgaat
     6541 cttgagttca gggggcaggt aaagttgttg aaaagagcgg gcaaattttc caatatcgtg
     6601 ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa tccctaattg
     6661 acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc ccatcgcggc
     6721 tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt tctcgccgcg
     6781 tcgggttttt ccccagaaat caaaaaaagt cacaatattt ttat
//
LOCUS       DAZPEP010000198         6696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZPEP010000198 DAZPEP010000000
VERSION     DAZPEP010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.199"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..737)
                     /locus_tag="ACHUT9_003886"
     CDS             complement(<1..737)
                     /locus_tag="ACHUT9_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HFR1350255.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUT9_003887"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="ACHUT9_003887"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFR1350256.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUT9_003888"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="ACHUT9_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFR1350257.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="ACHUT9_003889"
     CDS             4397..4678
                     /locus_tag="ACHUT9_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFR1350258.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            4970..5512
                     /locus_tag="ACHUT9_003890"
     CDS             4970..5512
                     /locus_tag="ACHUT9_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1350259.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            5592..6266
                     /locus_tag="ACHUT9_003891"
     CDS             5592..6266
                     /locus_tag="ACHUT9_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFR1350260.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            6282..>6696
                     /locus_tag="ACHUT9_003892"
     CDS             6282..>6696
                     /locus_tag="ACHUT9_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HFR1350261.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT         1771 a         1513 c         1601 g         1811 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg attttaattg catttagaaa gccgaaatca tttatataaa tgatttcggc
     4741 ttttttattg atatcaacaa taccatttac ataaatctac attaattgat agatgcgata
     4801 aatagttaat actcttaaat atttgttgta tttatattgc aatggttaat ttgtttcaaa
     4861 taaataaaac aatgtgttta cattgcggta acttttttga tgtgagaaat ttgtttccaa
     4921 gagatacaga ctcttaacaa ccaaaaaaaa ttttattaag gaacatatta tgaaacgttc
     4981 aattattgct gccgctgtct tttcttcttt ttttatgagc gctggagtat ttgctgcaga
     5041 cgttgatacc ggaacattaa ctattaaggg gaatattgca gaatctccgt gtaaattcga
     5101 agcgggtggt gattcagtaa gtattaatat gccgactgta ccaaccactg tctttgaagg
     5161 taaagctaaa tattctacct atgatgatgc agtcggtgta accagcagca tgttaaaaat
     5221 tagctgcccg aaagaagttg ctggtgtaaa actctcgttg attaccaacg ataaaataac
     5281 cggtaacgat aaggcgatag ccagtagcaa cgataccgtg ggttactatc tctatttagg
     5341 tgataacagc gatgtcctgg atgtttctgc accttttaac attgagagtt ataaaacagc
     5401 ggaaggtcaa tatgctattc cgtttaaagc aaaatacctg aaactgacag ataactcagt
     5461 gcaatcaggt gatgtgttat cttctctggt tatgcgtgtg gcgcaggatt aataaataaa
     5521 attgtaagtt tctccaggta ataaacatgg ccgctatccc atggcggcct tttaatttaa
     5581 gaggcattaa aatgaaagga ttattatctt tactcatttt ttctatggtc cttcctgcac
     5641 atgccggaat tgttatctac gggacgcgca ttatttaccc ggcagaaaat aaagaagtga
     5701 tggtgcagtt gatgaaccag ggaaaccgtt cttcgctgct gcaggcgtgg attgatgatg
     5761 gcgatacgtc attaccacca gaaaaaattc aggttccttt catgttaacg ccaccagtgg
     5821 caaaaatagg ggcaaattcc gggcagcaag taaaaatcaa aattatgccg aataaactgc
     5881 ccactaataa agaaagcatt ttttatctga atgttctgga tattccacca aatagtccag
     5941 agcaagaagg taagaatgca ctgaagtttg cgatgcaaaa cagaattaag ttgttttacc
     6001 gaccagcggg tattgctccg gtaaataaag cgacatttaa aaaattgctg gtaaatcgca
     6061 gtggcaatgg tttggtgata aaaaatgact cagctaattg ggtgacgatt tcggatgtca
     6121 aagctaataa tgtcaaagtc aattatgaaa ctattatgat tgccccctta gaaagtcaga
     6181 gtgttaatgt caaaagtaat aatgcaaata actggcatct gaccattatc gatgaccatg
     6241 gcaactatat tagtgacaaa atttaattgc aggagctgcc tatgttgaga atgaccccac
     6301 ttgcatcagc aatcgtagcg ttattgctcg gcattgaagc ttatgcagct gaagaaacct
     6361 ttgataccca ttttatgata ggtggaatga aagaccagca ggttgcaaat attcgtcttg
     6421 atgataatca acccttaccg gggcagtatg acatcgatat ttatgtcaat aagcaatggc
     6481 gcgggaaata tgagattatt gttaaagaca acccgcaaga aacatgttta tcaagagaag
     6541 ttatcaagcg gttaggcatt aatagcgata acttcgccag cggtaagcaa tgtttaacat
     6601 ttgagcaact tgttcagggt gggagctata cctgggatat cggggttttt cgtctcgatt
     6661 tcagtgtccc gcaggcctgg gtggaagaac tggaaa
//
LOCUS       DAZPEP010000199         6555 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZPEP010000199 DAZPEP010000000
VERSION     DAZPEP010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6555
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.200"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            144..1319
                     /gene="mdtL"
                     /locus_tag="ACHUT9_003893"
     CDS             144..1319
                     /gene="mdtL"
                     /locus_tag="ACHUT9_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFR1350262.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1294..2253
                     /gene="yidZ"
                     /locus_tag="ACHUT9_003894"
     CDS             1294..2253
                     /gene="yidZ"
                     /locus_tag="ACHUT9_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFR1350263.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2410..3159
                     /locus_tag="ACHUT9_003895"
     CDS             2410..3159
                     /locus_tag="ACHUT9_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFR1350264.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3181..3747
                     /gene="yieF"
                     /locus_tag="ACHUT9_003896"
                     /gene_synonym="chrR"
     CDS             3181..3747
                     /gene="yieF"
                     /locus_tag="ACHUT9_003896"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFR1350265.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(3801..5138)
                     /gene="adeP"
                     /locus_tag="ACHUT9_003897"
     CDS             complement(3801..5138)
                     /gene="adeP"
                     /locus_tag="ACHUT9_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFR1350266.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5304..5969
                     /gene="yieH"
                     /locus_tag="ACHUT9_003898"
     CDS             5304..5969
                     /gene="yieH"
                     /locus_tag="ACHUT9_003898"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFR1350267.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6036..6509
                     /locus_tag="ACHUT9_003899"
     CDS             6036..6509
                     /locus_tag="ACHUT9_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFR1350268.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1611 a         1655 c         1658 g         1631 t
ORIGIN      
        1 aaatttggct aaatccttca agaagccagc cattcgctgg cttcttgcct ctcaggaaat
       61 cacttatgtc caaatggcaa ctcgcctgat cctccttcac cacgtatgct ttgcgtcacc
      121 ttactatcag gacgctttag cccatgtccc gctttttgat ttgtagtttt gccctggttt
      181 tactttatcc cgccgggatt gatatgtacc tcgttggttt accgcgcatc gccgccgatc
      241 tcaatgccag cgaagcgcag ttgcatattg cgttctccgt atatctggcg gggatggcag
      301 ctgcgatgtt atttgccggt aaagtggctg atcgttcagg gagaaagccg gtcgccatac
      361 ccggcgcggc gctatttatt attgcctcgg tgttctgttc actggctgaa accagcacgt
      421 tatttcttgc aggccgattt ctacaggggt tgggcgcagg ctgttgttac gtagtggcgt
      481 tcgccatttt gcgcgacacg ctggatgatc gacgtcgggc taaagtgctg tcattactca
      541 acggtattac ctgcatcatt ccggtgttag cgccagtgct cggacatctg attatgctta
      601 aattcccgtg gcagagtctg ttctgggcga tggcaatgat gggcatcgcg gtactgatgt
      661 tgtctttgtt tattttaaaa gaaacgcgcc cagcgtcccc cgctgcttcg gacaaaccac
      721 gagaaaatag cgagtcgctg cttaatcggt ttttcctcag ccgtgttgtt atcaccaccc
      781 tcagcgtttc ggtgatcctc actttcgtca atacatcgcc ggtattgctg atggaaatca
      841 tgggttttga gcgcggagaa tacgccacca ttatggcgtt gaccgctggc gtcagcatga
      901 ccgtttcatt ctccacgcca tttgcgctgg gaatttttaa gccacgtacg ttgatgatca
      961 cctcgcaggt gttattcctt gcggcgggga tcacccttgc cgtttcacct tcccatgcgg
     1021 tttctctgtt tggtatcacg ctgatttgcg ccggtttctc ggtaggtttt ggcgtagcga
     1081 tgagtcaggc gttaggacca ttttcattac gcgcgggcgt agccagctcg accttaggta
     1141 ttgcgcaggt ttgcggttcg tcactgtgga tttggctggc agcggtggtt ggtattggcg
     1201 catggaatat gctgatcggg attctgattg cctgtagtat agtgagcctg ttgctgatta
     1261 tgttcgtcgc gcctggacgc cccgttgccg ctcatgaaga aatccatcac cacgcttgat
     1321 ctcaatctgc tgctctgtct gcaactgctg atgcaggagc gcagcgtgac caaagcggcg
     1381 aagcggatga acgtgacccc ttcagcggtg agtaagtcgc tggcaaagtt aagagcgtgg
     1441 tttgacgacc cgctctttgt gaactcaccg ctgggcctgt cgcccacacc gctaatggtc
     1501 agcatggagc aaaatctggc ggaatggatg caaatgagca atctgctgct ggataaaccg
     1561 caccaccaga caccgcgcgg cctgaagttt gagttggcgg cggaatcgcc gctgatgatg
     1621 atcatgctta atgcgctgtc gaaacggatc taccaacgtt acccgcaggc gaccatcaaa
     1681 ctacgtaact gggattacga ttccttagat gccattactc gtggtgaagt ggatattggt
     1741 ttttccggtc gcgaaagcca tcctcgctcg cgggagctgt taagctcgct accgttagcc
     1801 attgattatg aagtgctgtt tagtgatgta ccctgcgtct ggttacgcaa agatcatccg
     1861 gcactgcatg aaacgtggaa tctggacacc tttttacgtt atccgcatat cagcatttgc
     1921 tgggaacaga gcgacacctg ggcgctggac aatgtgttac aggagctggg gcgcgaacgc
     1981 acgattgcta tgagtctgcc ggaattcgag cagtcactgt ttatggcagc gcaacccgac
     2041 aatctgctac tggcgaccgc gccgcgctac tgtcagtact acaatcaact ccatcaactg
     2101 ccgttggttg ctcttcctct cccgtttgac gaaagccagc aaaaaaagct ggaagttcct
     2161 tttaccctgc tgtggcataa acggaacagc cataatccga agatcgtctg gttacgggaa
     2221 accattaaaa acctttacgc gtcgatggca taaccgaatc gtatgaaacg cgacccaatt
     2281 tcacaataaa atgtaaaaaa gttgtaacaa gcttgtctga atcgaacttt tagccgcttt
     2341 agtctgtcca tcattccagt aaatgattac tcttgtattc ataatggacc attaagcatg
     2401 gagcgaaaaa tggcgactca ctttgcccga gggattttaa cggaaggaca tctgatttct
     2461 gttcgtctcc cctcccagtg tcatcaagaa gcccgaaaca ttccccctca tcgtcaaagc
     2521 cgttttctgg cgtccagagg tttactcgca gaactgatgt tcatgctgta tggcattggc
     2581 gaattgccgg aaatcgtcac cctgccgaaa ggtaaaccgg ttttcagtga taaaaatttg
     2641 ccttcgtttt ctatttccta tgccgggaat atggttggcg tggcgttaac aaccgaaggt
     2701 gaatgtggcc tcgatatgga actacagcgt gcgacgcgcg ggtttcatag cccacacgcg
     2761 cccgataacc acaccttttc cagcaatgaa tcgctatgga tcagtaaaca aaacgatcct
     2821 aacgaagcgc gggcgcagct catcacgctg cgccgaagcg tgctaaaact aaccggtgat
     2881 gttttgaatg acgatccgcg cgatctgcag ctgctgccca ttgccggacg cctgaaatgt
     2941 gctcatgtaa atcatgtaga agcgttatgc gacgcggagg acgtgctggt gtggtccgtg
     3001 gcggtcacgc ccgcgattga aaagctcagt gtctgggagt tagatggcaa acacagctgg
     3061 aaaagcctgc cggatattca cagccgcgcc aacaatccta ccagccggat gatgcgtttt
     3121 gcccagctct ctaccgtgaa gtctttttcg ccaaattgat agacaaccac aggagtcatt
     3181 atgtctgaaa aattgcaggt ggttacgtta ctggggagcc tgcgcaaagg ctcatttaat
     3241 ggcatggttg cacgtaccct gccgaaaatt gctccggcga gcatggaagt caatgcgtta
     3301 ccttccattg ccgacattcc cctgtatgac gctgacgtac agcaggaaga aggttttcca
     3361 gcaacggttg aagctctggc ggaacagatc cgtcaggctg acggtgtggt gatcgtcacg
     3421 ccggaatata actactcggt accgggtggg ctgaaaaatg ccatcgactg gctttcccgc
     3481 ctgccggatc aaccgctggc cggtaaaccg gtgttgattc agaccagctc aatgggcgtg
     3541 attggcggcg cgcgctgtca gtatcacctg cgccagattc tggttttcct cgatgcaatg
     3601 gtgatgaaca agccggaatt tatgggcggc gtgattcaga acaaagttga tcggcaaacc
     3661 ggagaagtga ttgatcaggg tacgctggac cacctgaccg ggcaattgac cgcatttggt
     3721 gagtttattc agcgagttaa gatctaaata aaaaacccgc cagtaatcat gcatggcggg
     3781 tttttaatgc gctatcgatt ttaatgagcg tcgataaata caatcttcag gataaacagc
     3841 agcgcaacga tgattacgca cgggctaagg tcacgcagac gcccggtacc aatcttcatc
     3901 acgcagtagg agataaagcc cagcgcaata ccttcggtaa tcgaaaagct gaacggcatc
     3961 atcacggcgg taataaacgc cggaacagat tcagtaagat cctgccagtt cacgcgagca
     4021 agacttgagg tcatcaacac gccaacgtag atcaacgcgc cagctgcagc gtagcctggc
     4081 accattcccg ccagcggcga cagaaagata accagcagga acagcagacc aacaaccact
     4141 gccgtcagac cggtacgacc gccaaccgat acgccggaag aggactcaat ataagccgta
     4201 acggaagaag taccgataaa cgaaccggtc acggaagaga tactgtcgac atacagcgcc
     4261 tgcttcatgc gcgggaattt ccccttctca tccgccagac ctgctttatc ggtcacgcca
     4321 atcagcgtac cggaggagtc aaacaagttg accaacatga aagagaaaat cacccctgcc
     4381 agcccgaggt taaacgaccc ggctaaatct acatgaccca caactgtcat tacgctcggc
     4441 ggcgcagaaa cgatgccatt gtagtgcaca tcacccagca tccagcccag cagcgtcgtc
     4501 accacgatag aaaccagcac cgctgcgtga atgttgcgcg aggccagaat ggcgatgatg
     4561 aagaaaccga ggatccccag cagcacgctg tgagaagtca gattaccgat gctcaccagc
     4621 gtttccgggt tagcgacaat cacacctgcg tttttcagcc ccatcatgcc aatgaacaga
     4681 ccgataccgc tggtaatacc cacacgcaga cttaccggaa tgttagcaat catccagtaa
     4741 cgaacgcgga aaatggtcaa aagaagcagt ccgacagcgc cccagaagat tgcgcccata
     4801 ccgatctgcc acggcagccc cattgcctgg acaaccacaa aagcgaaaaa ggcgttaagc
     4861 cccatcgcag gagccagcgc caccggaagg tttgcaaaca atcccatcat aatgctaccg
     4921 aaagcagcaa tcagacaggt tgttacgaag acggcactgg tgtccatgcc agcaacgccc
     4981 agaatttgtg ggttaacaaa tacgatgtag accatcgtca ggaaggtggt gaacccggca
     5041 atcacttcag ttcgtgccgt agtaccatgt tcgcgcagtt taaaaactcg ttccagcatc
     5101 ccctgaccag aagtctgggt ggtatgttga tgactcatta tctatttccg aacaaggagg
     5161 gaaaatccgt cgctatcgta taccaaaatg cgacaatagg cgcgtttgtg agagactttt
     5221 tttattggat ttacttatac ggcaacgatt gcgttgcgca aatcggctct acgaaaacgt
     5281 taaactgatt aaaaaggaaa ggcatgtccc ggatagaagc ggtatttttc gactgcgacg
     5341 gtacgctggt cgacagtgaa gtcatttgct cccgcgcata tgtaacgatg tttcaggaat
     5401 ttggtattac gctcgatcct gaagaggtat tcaaacgttt caaaggtgta aaactgtacg
     5461 aaattatcga tattgtttcc cttgaacatg gtgttacgtt agcgaaaaca gaatctgaac
     5521 acgtttaccg tgcagaagtc gctcggctgt tcgattcaga actggaagcc atcgaagggg
     5581 ctgaagcgct cctgtcagcg atcactgcgc caatgtgtgt ggtatctaac ggcccaaata
     5641 acaaaatgca gcattctatg ggcaagctga atatgttgca ctacttcccg gataaactgt
     5701 tcagcggcta cgatattcag cgctggaagc cagacccggc gttaatgttc catgcggcaa
     5761 aagcgatgaa tgtaaatgta gaaaactgca ttctggttga tgactcagtt gccggtgcgc
     5821 aatctggtat cgacgcaggt atggaagtgt tctacttctg cgccgacccg cacaataagc
     5881 cgatcgttca cccgaaagtc accaccttta cccatctttc gcagttaccg gaactgtgga
     5941 aagcgcgtgg ttgggatatt acggcatagt tcttcacact cccttcactt accccgctta
     6001 aattggcgct caaaggtaag taaagggagt ttgatatgtc tgtttcacgt cgggtaatac
     6061 atcacggact ttattttgcg gttttaggac cgttaattgg tgttctgttt cttgtcctct
     6121 acatattctt cgcaaaagaa ccgctggttc ttttggtaat aatacaggta cttccattgt
     6181 ttctcttatt gtcgattact acgggagcta ttcctgcatt gttaaccggc gtgatggtcg
     6241 cctgtctgcc ggaaaagatc gggtcacaga aaaattatcg ttgtctggct ggtggcatag
     6301 gtggcgtcgt tatcaccgag atctattgtg cagttattgt acatattaag ggcatggctt
     6361 cctcggagtt gtttgaaaac attctttctg gtgacagtct cgttgtccgc atcattcctg
     6421 cattgctggc aggtgtggtg atgagcagaa tcattacccg tctgcccgga ttggatattt
     6481 catgtcctga aacagactct ttaagttaag cgggatactt tatctttggg ctactcaaaa
     6541 gcagacagga tgtta
//
LOCUS       DAZPEP010000200         6499 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZPEP010000200 DAZPEP010000000
VERSION     DAZPEP010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.201"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUT9_003900"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="ACHUT9_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="ACHUT9_003901"
     CDS             complement(1751..2509)
                     /locus_tag="ACHUT9_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFR1350269.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUT9_003902"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="ACHUT9_003902"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFR1350270.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUT9_003903"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="ACHUT9_003903"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFR1350271.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUT9_003904"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="ACHUT9_003904"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFR1350272.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="ACHUT9_003905"
     CDS             complement(5474..6079)
                     /locus_tag="ACHUT9_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350273.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..>6499)
                     /locus_tag="ACHUT9_003906"
     CDS             complement(6082..>6499)
                     /locus_tag="ACHUT9_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350274.1"
                     /translation="QRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINSV
                     PGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIENP
                     LKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
BASE COUNT         1638 a         1695 c         1663 g         1503 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgccagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgt
//
LOCUS       DAZPEP010000201         6462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZPEP010000201 DAZPEP010000000
VERSION     DAZPEP010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.202"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..699
                     /locus_tag="ACHUT9_003907"
     CDS             <1..699
                     /locus_tag="ACHUT9_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1350275.1"
                     /translation="TPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKKLARL
                     QRQLSRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVS
                     NMSKSAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYT
                     SQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQSGR
                     PLKQEPTEMIQATA"
     gene            complement(739..1410)
                     /locus_tag="ACHUT9_003908"
     CDS             complement(739..1410)
                     /locus_tag="ACHUT9_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFR1350276.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1517..1750)
                     /gene="yedF"
                     /locus_tag="ACHUT9_003909"
     CDS             complement(1517..1750)
                     /gene="yedF"
                     /locus_tag="ACHUT9_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFR1350277.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1747..2952)
                     /gene="yedE"
                     /locus_tag="ACHUT9_003910"
     CDS             complement(1747..2952)
                     /gene="yedE"
                     /locus_tag="ACHUT9_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFR1350278.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            3139..3552
                     /gene="yedD"
                     /locus_tag="ACHUT9_003911"
     CDS             3139..3552
                     /gene="yedD"
                     /locus_tag="ACHUT9_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFR1350279.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3586..5073)
                     /gene="amyA"
                     /locus_tag="ACHUT9_003912"
     CDS             complement(3586..5073)
                     /gene="amyA"
                     /locus_tag="ACHUT9_003912"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFR1350280.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(5151..5516)
                     /gene="fliT"
                     /locus_tag="ACHUT9_003913"
     CDS             complement(5151..5516)
                     /gene="fliT"
                     /locus_tag="ACHUT9_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFR1350281.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5516..5926)
                     /gene="fliS"
                     /locus_tag="ACHUT9_003914"
     CDS             complement(5516..5926)
                     /gene="fliS"
                     /locus_tag="ACHUT9_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFR1350282.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5941..>6462)
                     /gene="fliD"
                     /locus_tag="ACHUT9_003915"
     CDS             complement(5941..>6462)
                     /gene="fliD"
                     /locus_tag="ACHUT9_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1350283.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1604 a         1697 c         1609 g         1552 t
ORIGIN      
        1 actccggttc acccttcagc atcaatggtc gggctggatg ctggcgtggc taaactcgcc
       61 acgctgtcag atggcacagt ctttgagcct gtaaacagtt ttcagaaaaa ccagaagaag
      121 ctggcgagac ttcagcgaca gttaagccgc aaggtcaaat tcagcaacaa ctggcagaag
      181 cagaaacgca aaatacagcg actgcattcc tgtatcgcaa atatccgcag ggactacctt
      241 cacaaagtca caacgaccgt cagcaaaaac cacgcaatga tagtcattga ggatttgaag
      301 gtcagcaaca tgtcaaagtc agcagcgggt acggtcagcc agccggggcg caatgtccgg
      361 gcaaaatcag gtttaaaccg ttcgatactg gatcagggct ggtatgaaat gcgccgccag
      421 cttgagtaca agcagctctg gcgtggcggt caggtgcttg ctgttccgcc agcgtacaca
      481 agccagcgtt gcgcgtgctg tggtcataca gcgaaagaaa accgcctgtc acaaagtaaa
      541 ttcagatgcc aggtatgtgg atatacagtg aacgccgatg taaatggcgc tcgtaacatt
      601 ttagcggcgg ggcacgccgt tcttgcctgt ggagagatgg tgcagtcagg ccgcccgttg
      661 aagcaggaac ccaccgaaat gattcaggcg acagcctgaa cgtagcaggg atcctcgtcc
      721 ttcagggcgt ggaggatgtc aacaaacagg ttgaattaac tccgcgccct ggtttttgac
      781 attacctacc gcgcgcgata ccgggtgcca ggtgaactgg tttgctggca cacagccgtt
      841 ggtagcaatt tctgatgctt ctttaccgcc aatctcttgc cgcatccatt cctgtgcagc
      901 ttctggcgac agaaccagcg ggcggcggtc atgaatgtct accagaccct ggtctgctgc
      961 agcagtgaca attaaaaatc cttcggcttc gtcaccacgc tcaaatggtg tgctacctat
     1021 cgcggccata aaaataggtt gtccatcagc acgatagata aaaaaaggct gttttttgtc
     1081 gccttctttt ttccactcaa accagccatc ggcaaaacag attgcccgac catgttgcca
     1141 gagcggttta aacatgcgac tggttgcggc agtttctacg cgggcgttaa tcagcggcgg
     1201 tttatcccac catccgggag catatcccca gaaaaccgga tccagatgaa ggtgttcatc
     1261 acgttcactg agcagaagaa ctttggttcc cggcgcgacg ttgtatctgc caatgggttc
     1321 gggatcgtag ggaatatcgc gttcaatatc ttccgcgaga agcgcaaggt aatcttcacg
     1381 cgtttgggat tgggcaaagc gtccacacat agaaacctcc tgagatcata ctgaaaagaa
     1441 taggagaggg ggcgggtaaa atctatgtgt cagaggcaac aatgttcccg ctgtgggcca
     1501 cagcgggaat gagggattac ttctgaatta aataacgaat ggtcggcccg tcttgctgaa
     1561 tatccagcac cgtatagccg tgattacgcg catccagtgg aatattattg atcgactgtg
     1621 gacagtcgct caccacttcc aggatttccc ctttttttaa ctgcggcatc gcctcaaggg
     1681 ttgcgactgc cggataaggg cagggttcac ccaccatatc aaggcggtaa tcaggaacga
     1741 tatttttcat gcgatctcct tagcagtctg cggtgccgca cggcggaaga agcgtttttc
     1801 ccagccgata atcaacatca gcgcagtaaa caacagcaaa tatgtcacca gcaggccacc
     1861 catcgggcca aaggttttca gcaggttgat tttgtcccag tcggtggcca gcgccggagc
     1921 gaaatcatcc cagtaatacg ccagaatcgt cgagccgatc acattgccca gaccgaccca
     1981 ccagtagtgc acctggcctt ctaccgcgcg gtacatccag ccggtttcgc agccgcctgc
     2041 cagcacgatg ccaaaaccaa acagtaaacc accaattacc gcgtttggtc ccgcccacat
     2101 gattttgggt tcaacgccta actgtacgta actgaagatc ccgatggcac tcaccgccat
     2161 accgataatg attgctttcg ccatatgggt acgtccggtg atccacatat cgcggaacgc
     2221 cgaagtaaag cagatttgcg cacgttcaat cagtaaacca aagccgacgc caaacagcat
     2281 tgccagcccc agtttgggtt ggttcatcgc cgtgagcagc gcccagccca gcatgccgaa
     2341 aaagaccagc atcccgagac gaaaacgacg ccgcgcctga tccggttttt gcgtcagcgg
     2401 tgaggcggca gaaactttct gcattttcac gggaatacgg aagatgggca gcagggtaaa
     2461 gcgcgcgcca aaccatgaac caatggcagt ggcgatggca aagaaccagg catgcagcga
     2521 gaactgagga atgccggtaa agaacgccgc caggttacag cccattgcca gacgcgcgcc
     2581 aaaaccggcg ataatgccgc caataatggc ctgcataatg cggatacggc tgcgcggcat
     2641 gcgcagtttg acattgttgg cccacagcgc tgcggcaaag cagccgccaa acatgccgag
     2701 gatcatcatc ccgtcgatgc gggttaatgg cgatccttcc agatggataa ttttaaaata
     2761 accccactct tcagcatgga cgccaaacag ctgcaggagc tgaccgcccc aacgggtaaa
     2821 ttcacccgtg acagcccaaa aggtgccagt aatgccaaaa tagtaagtgg agagaatacc
     2881 cgccgcgatg accgcaggga tgggtgccca gaatttaatc aaccaggcgt gtttgaattg
     2941 ctgccatgac atgaatgtag cctctgaact cagaaggttt gaaacaagaa tcgcgaatca
     3001 tacatcatgc aaatttattt tcggggtaat aattcacaaa aataatagtt agatcaaatt
     3061 tctctctgaa cctggaaagg ctggcgacat cagggcttta atgccacaat cctcttttct
     3121 tctcatgcag gacgaatgat gaaaaaactc gcaattgcag gtgcgctcat gttgctggcg
     3181 ggctgtgccg aagtcgaaaa ctataacaat gtcgtgaaaa cccccgcgcc ggactggctg
     3241 gcgggctact ggcagaccaa agggccgcaa cgtgcgctgg tcagtccgga agcgataggc
     3301 agcctgattg tcactaaaga gggcgatacc ctggattgcc gtcagtggca gcgggtgatt
     3361 gccgtgccgg gcaagttgac attgatgtct gatgatctca ctaacgttac cgtaaaacgt
     3421 gagctgtacg aggttgagcg tgacggtaac accattgagt acgatggtat gacgatggag
     3481 cgtgtggatc gcccgacggc agagtgtgcc gccgcgctgg ataaagcgcc gttaccgacg
     3541 ccactgccgt aatgaaatct ccccggcttg ccgccgggga tgaatttaaa tcacctcttc
     3601 gataacccac acgctgacgc tgccgccgtt gcaaaagaag gtcgcttcgc cgttttcgtc
     3661 ggtcactacg ctctcttgcc gattccccag aaaatcacgc caggttttat tgccgtaatt
     3721 ctctcccaga tgaatggttt tttcgccatc atccccgttc gacatgacca ccacgcagcc
     3781 gggatattcg tcagtgccac tgcggctaaa ggcaatgcag ttcggatggt cgaaaaataa
     3841 cgtctgtaca ccgtgggcga aacgctgacg ggcgagaatt aactcatcaa gctgttcgat
     3901 tattggcata tctatcggat aggtttgccc gtcaccaccg acatcttcgt aatgcgcacc
     3961 gtagaggtcc ggatagaaca ccgaaggaac gccattttcc cgcaacaaaa ttagagcata
     4021 cgccagcggt ttaaaccacg gttcgaccgg cgcttcgagg gcttgcaacg gttgggtgtc
     4081 gtgattggct acgagggtta cggcgtggaa aggatcggct tccactaatg tacccgtgaa
     4141 aatctgcgtc atgtcgtagt cgcgtcccat gcgcgatgct tcatggaatt tcatctgcag
     4201 cggcgcatcg aacagcatgg ttttgccttc cacctgatca atatacgttt gcagtttatc
     4261 gacttcatgc gaccagtact ctgccacaat aaacagcggc tttggcgcaa cttcctgtac
     4321 gtgttcgatc cactctttat aaaaccacgc cggaatatgt ttaaccgcat caagacgaaa
     4381 accgtcgcat tgtgtttgtt ccatcaccca gcgcgcccag tatttaatct cttccgtcac
     4441 ggcatgattg cggaaatcga tattctcacc catcagataa tcgaaattac cgagttcatc
     4501 atcaacctga tcgttccagc cttcgccggt gtagtcgtta acaattttaa atatgccatc
     4561 ttcatcagga ttttcgatat ggtcgatacc gctaaaacat ttgaagtccc agataaactg
     4621 cgagtattgc ccggcacggg cggggaaggt gtaacgcgtc cagccttcgc actcaatgat
     4681 ttcttcatca atttgcgtac ggtcatcagc atttacacgc tgcacgcgaa tagcttcttt
     4741 ttcatccgcc cccattttgt ggttgactac cacgtccagt agcaccgcaa tgtcattacg
     4801 tttcagagcg tcaatggcgg ccagcagttg tgctttatcg ccatatttag tagggatgct
     4861 gcctttctga tcaaactcac ctaaatcaaa taaatcatag gagtcgtagc cgaccgaata
     4921 cccgcccgat gcgcctttat aggcgggcgg caaccagacc atattgatac caatatcatt
     4981 aaaaccgtcg gcgcgctcgg ccagttcagg ccagagctta ccgccttccg ggtaatacca
     5041 gtgaaaacat tgtaacagcg tgggattacg cattcttcga ctccattcaa ggggaacatt
     5101 agaagcgtag ccgtaatcgg attattcgcg agccatcggc tcgttaagat tcaaaagagg
     5161 ttatcctgcg gagccagcac aaagccgccc tgatctccat aggcacttaa cactgatttt
     5221 tgcaccgatg actgaccgac cagtttcgcc agttcatcca tacgaatctg taataactgc
     5281 tttaccttgc tttcgttgtc gagaatcagg cgcagcatcg ggcggagctg ctcctgcatc
     5341 gtggtggacg gggcaacctc ttcagtcaga tgtgcaatct cctgcaccgc attcacatac
     5401 gccatttcgc tggcgatgag ttcgtcccat tgttcttccg ttgccaggcg taacatgagc
     5461 tggcttttat cgacgagttg ttgccaggcg aaatacaaat gcggtgcatg gttcatcaga
     5521 ctgggtcctg aatcaaagaa ggggagagta aagactcttt ccaggcatcg gcaatattgc
     5581 gcattaatgc ttccacttct tcgactgcgg agacatcgtt gcgtaaattg gcttgcagca
     5641 agcgcctgac catatagcta taaagagcaa tcaagttttg ggttagttcg tctttgctct
     5701 cttcatcaag actcacccgc agcccgttct caatgatgtt gatcgctttt gacaaagaga
     5761 cgcctttgcc ttgctgattg ttgtcctgca taaacaggcg cgctctaacc agtgcgctca
     5821 gcactccatc aaatagcatg gtgaccagct gttgctggct ggcgctcatt acggcgcttt
     5881 cgacgccaat ttgtgcatag gcttttgagc cgctcgcggt atacatgctg acctccgtga
     5941 tcaggaatta ctcattgcag taaattgttg gctgagatag gtactggtgt tattcagttt
     6001 gctcatcatg gtatctaatt gcgtaaactg tgcagtgtaa cgcgccacgg tgtcatcaat
     6061 gctggcactg acggatagat actgtttggt cagttttttc agtgtggcgt taatactgtc
     6121 ctgtgcgcta tcaataatgc catcatcggc aaggtaacct tttacctcgg tggcaatttt
     6181 ggtggtaatt cctgtttctt taccatcgcc taccagcaat tcacgtacgg atgctgtatt
     6241 ttcattcagg acttttttga gtttatcatc atcaattttc agcttacccg tcgtgccgtc
     6301 ctgcttaatc ccgatctcgt ttaatgtttt gaaggcactg tcgctggcac cgtttgcaaa
     6361 ttgtgcgcgg atgccgcttt gaattgtacg tacaacgctg tctccgagaa gtgcaccatt
     6421 atttttatct tgtgcttcag cacctgggtc tacttcttta ta
//
LOCUS       DAZPEP010000202         6333 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZPEP010000202 DAZPEP010000000
VERSION     DAZPEP010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.203"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            190..2037
                     /gene="ldtD"
                     /locus_tag="ACHUT9_003916"
     CDS             190..2037
                     /gene="ldtD"
                     /locus_tag="ACHUT9_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFR1350284.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2218..2766
                     /locus_tag="ACHUT9_003917"
     CDS             2218..2766
                     /locus_tag="ACHUT9_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFR1350285.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2793..3440
                     /gene="gloC"
                     /locus_tag="ACHUT9_003918"
     CDS             2793..3440
                     /gene="gloC"
                     /locus_tag="ACHUT9_003918"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFR1350286.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHUT9_003919"
     CDS             complement(3662..4852)
                     /gene="aspC"
                     /locus_tag="ACHUT9_003919"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFR1350287.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHUT9_003920"
     CDS             complement(5037..6125)
                     /gene="ompF"
                     /locus_tag="ACHUT9_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFR1350288.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1589 a         1557 c         1607 g         1580 t
ORIGIN      
        1 tacggcgcgc ttttctttaa acttctttac attaggttat gtaaaaacgt atcggcgttt
       61 atatactgaa gataagcctg atgagtaaca ggcttgctcg tcatactttc gtgagtattg
      121 gcgttgtaca ggcaagtcgt aaaataacag cctggctatt cagagtatga taaaaacagg
      181 gggcaaggga tgttgcttaa tatgatgtgt ggtcgtcggc tgtcggcaat cagtttgtgc
      241 ctggccgtaa cattcgctcc actgttcaat gcgcaggccg atgagcctga agtaatccct
      301 ggcgacagcc cggtggctgt cagtgaacag ggcgaggcac tgccgcaggc gcaagccacg
      361 gcaataatgg cggggatcca gccattgcct gaaggtgcgg cagaaaaagc ccgcacgcaa
      421 atcgaatctc aattacccgc aggttacaag ccggtttatc ttaaccagct tcaactgttg
      481 tatgccgcac gcgatatgca acccatgtgg gaaaaccgtg atgctgttaa agccttccag
      541 caacagctgg cagaggtggc gattgccgtt ttccagccgc agtttaataa atgggtagag
      601 ttactgaccg atcctggtgt taacgggatg gcacgcgacg tagtgctctc tgatgcgatg
      661 atgggctatc tccatttcat tgcaaatatt ccggtcaaag gcactcgctg gctatatagc
      721 agtaaacctt atgcacttgc aacgccgccg ctctcggtga ttaaccaatg gcagctggcg
      781 ctggataaag gtcaattgcc tacgtttgtt gcaggactgg caccgcagca tccgcaatat
      841 gcggcgatgc atgaatcgtt actggcctta ctcagtgaca ccaaaccgtg gccccaactg
      901 accggcaaag caacgttgcg cccagggcag tggagtaacg acgtaccggc gttgcgcgaa
      961 atattgcaac gcacaggcat gttggacggg gggccgaaaa ttactctacc tggcgatgac
     1021 acgccaactg acgcggtagt cagcccatcc gctgttactg ttgaaacagc agaaactaag
     1081 ccgatggaca agcaaacgac gtctcgtagt aaacctgcgc ctgccgttcg cgccgcctac
     1141 gataatgaac tggtggaagc cgttaaacgt tttcaggcat ggcaaggatt gggggcagat
     1201 ggtgctattg gcccggcaac acgtgactgg ttaaacgtaa cgcccgccca gcgtgctggc
     1261 gtgttggctc tcaacatcca gcgattgcgc ttgctgccaa cagagctttc taccgggatc
     1321 atggttaaca ttccggccta ttcgctggtc tactatcaga acggcaatca ggtgctggat
     1381 tcgcgagtca ttgtcggtcg ccccgatcgc aaaacgccga tgatgagcag tgcccttaac
     1441 aacgtagtgg taaacccgcc gtggaacgta cctccaactc tggcacgcaa agatattctg
     1501 ccaaaagtgc gcaacgatcc gggatatctc gaaagccatg gctatacggt gatgcgcggc
     1561 tggaacagca gagaagcgat tgacccatgg caggttgact ggtctacaat cacggcctcg
     1621 aatttaccgt tccgcttcca gcaggctcca ggcccacgga actcgctggg gcgctataaa
     1681 ttcaatatgc cgagttcaga ggccatttat ttgcatgaca cgccgaacca caatctgttc
     1741 aagcgtgata cacgcgcatt gagctcaggc tgtgtacgag tgaataaagc ttccgatctg
     1801 gcgaatatgc tgttgcagga tgcaggctgg aatgacaaac gtatttctga tgcgctgaag
     1861 cagggtgata cacgttacgt caatattcgg cagtcgattc cggtgaatct ctactacctg
     1921 acggcctttg ttggtgcaga tggtcgtacc cagtatcgta cagatattta caattatgat
     1981 ctgcctgcgc gatccagctc gcaaatcgta tcgaaagcgg aacaattaat caggtaaatg
     2041 aagtagttcg gggaaatcag ttgtcgtaat aattagcatg attgggggcg attctctgca
     2101 gcccccgtca ctgctggggt tgagtcatct tgacgtctgc tttacgggcg gttaaggtgc
     2161 ctcttgtgcg ccagaagtgc atataaacga taacattgac ctgtagactt gattatcatg
     2221 gacaaattcg acgctaatcg ccgcaaattg ctggcgcttg gtggcgttgc actcggtgcc
     2281 gccatcctgc cgacccctgc gtttgcaaca ctctctaccc cacgcccgcg cattttgaca
     2341 ctcaataatc ttcataccgg agagtcaatc aaagcggagt ttttcgatgg cagaggctat
     2401 attcaggaag aattggcaaa acttaaccat tttttccgcg attaccgcgc gaacaaaata
     2461 aagtccatcg acccaggatt attcgaccag ttgtatcgcc tgcaagggtt gttaggcacg
     2521 cgcaaaccgg tgcaactcat ttccggttat cgttctattg ataccaacaa tgaactacgc
     2581 gcccgcagcc gtggagtagc gaagaaaagc tatcacacta aaggccaggc gatggatttc
     2641 catattgaag gtatcgcgtt aagcaatatt cgcaaagccg cgttatctat gcgcgcaggt
     2701 ggtgtaggat attatccacg tagtaacttt gtgcatattg ataccgggcc agcacggcac
     2761 tggtagtaat tgcttaacga aacaggggca gtatgaacta tcgtattatt ccggtcaccg
     2821 cattctccca gaactgttca ttaatctggt gtgaacaaac ccgtctggcc gcactggtcg
     2881 atcctggcgg cgatgcggaa aaaatcaaac aggaagttga tgacagcggc ctgacactga
     2941 tgcagatcct gctgacgcat ggtcatctgg accacgttgg cgcagcggcg gaactggcgc
     3001 aacattacgg cgtgccggtt tttggcccgg aaaaagaaga tgagttctgg ctgcaaggct
     3061 tgcctgcgca aagtcgtatg tttggtctgg aagagtgcca gccgctgacg ccagatcgtt
     3121 ggctgaacga aggcgatacc atcagcatag ggaatgtgac tttacaggtg ttacattgcc
     3181 ctgggcatac gccgggtcat gtcgtgtttt ttgatgatcg ggcaaagctg ctgatttctg
     3241 gcgatgttat tttcaaaggc ggagtagggc gcagtgactt cccgcgtggc gatcataatc
     3301 aactgatttc ttcaatcaaa gataaattgc tgccactggg ggatgacgtg acatttattc
     3361 cgggtcacgg accattatcc acacttggtt atgaacgcct gcataatccc ttcctgcaag
     3421 acgaaatgcc cgtctggtaa ggcacataaa aaagcccgct tttaatgcgg gcctggattt
     3481 ctggcaaagt gcgctttgtt tatgccggat gcggcacgag cgccttatcc ggcctacaaa
     3541 atcgtgcaaa ttcaaaatat tgcaggggac gcgtaggcct gataagcgta gcgcatcagg
     3601 caatgttgcg tttgtcatca gtctcagccc gcttttcagc gggcttcatt gtttttaatg
     3661 cttacagcac tgccacaatc gcttcgcaca gcggagccat gttatctggt gtcatcccgg
     3721 ccacatttac gcgaccagaa gcaaccgcat atacgccaaa ctcttcgcgc agacgcagca
     3781 cttgttcttt tgtcaggcca ctgaaggaga acatgccgtt ctgtttgatg ataaagctga
     3841 agtcgcggtt tgcgcctttt tcctgcagcg tattgacgaa caactgacgc atacgctgaa
     3901 tacgctggcg catatcagtc agctcttgtt cccaaatcgc acgtaacgca tcgttgctca
     3961 ggatggtggc aacaacagaa gcgccgtgtg ctggtgggtt agagtagtta gcgcgaatcg
     4021 ccgctttcat ttggctgaat gcgcgatcaa cggtttcact gtcggcagca accagagtac
     4081 aagcgccaac acgctcgttg tacaggccaa agtttttaga gtaggaactg gcaacaatca
     4141 gctctttatg catagccgcg aaagcgcgca gtccttcagc atcttcttcc agaccacggg
     4201 caaaaccctg gtaagcgaag tcaaacagcg gtaaccagcc tttctcaacg gagagttgtg
     4261 ccagtgtctg ccattgttcc agcgtagggt cgataccggt tgggttatgg cagcagccat
     4321 ggaacagcac tacgtcgcca gcctgagctt cattcaggct gttaatcagt gcatcgaagt
     4381 caagagtgtg attttccgca tcataataag cgtattcacg aacttccaga cctgcagagt
     4441 taaagacgct cttatggttc ggccagcttg ggttgctcac ccacactcgc ttaacgctgg
     4501 tattttttgc caggaaatcg gcagctacgc gtagtgcgcc agtgcccccc ggagtctgtg
     4561 ccgtgcgagc acgtttgtca ttgatcaggg cgctaccttt accaaacagc agttcctgag
     4621 tgcagcgacc aaattcaggg atgccgtcaa tgccgaggta atttttggtg gtttcatttt
     4681 cgagcagata ctgttcagcc tttttcacgc tggtcagtac cggggttttg cccgtctcat
     4741 ctttatagac accaatcccg aggttaattt tgccgggacg ttcatcggca cgaaacagat
     4801 cggccaggcc cagaatcggg tcggcaggag cggcggtaat gttctcaaac atgacgaggt
     4861 tccattatgg ttacagaagg gaagtccgct atcagggtaa cgggagattt acaaaattcc
     4921 aactattact gatgaaaacg caggctgttt ttgcaagacg tgagattgct ctggaaggta
     4981 taaaaaaaac aggaccaaag tcctgttttt tcggcattta acaaagaggt gtgctattag
     5041 aactggtaaa cgatacccac agcaacggtg tcgtctgaac ctacgcccag tttgttgtca
     5101 gaatcgatct ggttgatgat gtagtcaaca taggtggaca tgtttttgtt gaagtagtag
     5161 gttgcgccca cttcaaagta gttcaccagc tcaacatcac cgataccttc tacgtctttc
     5221 gctttagatt tggtgtaagc gatggacgga cgcagaccga aatcgaactg gtattgcgca
     5281 actaacagaa cgtcttgcgt tttgttggcg aagccgctgg tgtttgtaaa tttattagtg
     5341 atcggcgtag cgttacgggt ttcaccgtag ttcgctgcca ggtagatgtt gttcgcgtcg
     5401 tacttcagac cagtagccca ctgttcagct tttttaccgt tgccaagagg ttgagcttct
     5461 tgcaggttgg tacggtcagc tgcaccataa gcaccaacga taccaaagcc ttcgtattcg
     5521 tagctgatag aaccgccaac accgtcgccg ttagaacggc gtgcagtgtc acgctcgttt
     5581 ttacccaggt actgaacagc gaagttcagg ccatcaacca gaccaaagaa gttggagtta
     5641 cgataggtag caacgccgcc aacacgacca acgaagaagt catcgctgta tgcagtatca
     5701 ccaccaaatt ctggcagcat atcggtgtaa cccagtgcat cataaaccac accgtagtta
     5761 cggccgtaat cgaaagaacc aacgtcagcg tatttaagac ccgcgaatgc cagacgcgtt
     5821 ttgttaccag tttgagcgtc agcgccttca gagttgttac cctggaagtt atattcccac
     5881 tgaccataac cggtcagatc ggaattgatt tgagtttccc ctttaaaacc aagacgggca
     5941 taggtcatgt cgccattgcc accgtaactg ttttcaccgt tacccttgga aaaataatgc
     6001 agaccaacag ctttaccgta cagatctact ttgttgccat ctttgttata gatttctgca
     6061 gcgtttgcag tacctgctac taacagagca gggacgatca ctgccagaat attgcgcttc
     6121 atcattattt attaccctca tggttttttt tatgacacct gccactgcgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctacaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taa
//
LOCUS       DAZPEP010000203         6317 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZPEP010000203 DAZPEP010000000
VERSION     DAZPEP010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.204"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            77..1006
                     /gene="pfkB"
                     /locus_tag="ACHUT9_003921"
     CDS             77..1006
                     /gene="pfkB"
                     /locus_tag="ACHUT9_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFR1350289.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            1107..1397
                     /gene="ghoS"
                     /locus_tag="ACHUT9_003922"
     CDS             1107..1397
                     /gene="ghoS"
                     /locus_tag="ACHUT9_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFR1350290.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            1503..2363
                     /locus_tag="ACHUT9_003923"
     CDS             1503..2363
                     /locus_tag="ACHUT9_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFR1350291.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(2404..2940)
                     /locus_tag="ACHUT9_003924"
     CDS             complement(2404..2940)
                     /locus_tag="ACHUT9_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFR1350292.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            3087..3755
                     /gene="hxpB"
                     /locus_tag="ACHUT9_003925"
     CDS             3087..3755
                     /gene="hxpB"
                     /locus_tag="ACHUT9_003925"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFR1350293.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3918..4508
                     /locus_tag="ACHUT9_003926"
     CDS             3918..4508
                     /locus_tag="ACHUT9_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFR1350294.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            4641..6032
                     /gene="tcyP"
                     /locus_tag="ACHUT9_003927"
     CDS             4641..6032
                     /gene="tcyP"
                     /locus_tag="ACHUT9_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFR1350295.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(6036..>6317)
                     /gene="ydjO"
                     /locus_tag="ACHUT9_003928"
     CDS             complement(6036..>6317)
                     /gene="ydjO"
                     /locus_tag="ACHUT9_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjO"
                     /protein_id="HFR1350296.1"
                     /translation="NINLLFNPLNLPGMGSGVLEDIMSIPDSSLRKRLGYEVLSFSLQ
                     AHSLSQECIDKLDIFFADDLFKYESVCIAAMEHLKSKATAPIQNGPLPA"
BASE COUNT         1550 a         1549 c         1582 g         1636 t
ORIGIN      
        1 caataaatca tattgttaat ttcttcactt tccgctgatt cggtgccaga ctgaaatcag
       61 cctataggag gaaatgatgg tacgtatcta tacgttgaca cttgcgccct ctctcgatag
      121 cgcaacaatt accccgcaaa tttatcccga aggaaaactg cgctgtaccg caccggtgtt
      181 cgaacccggg ggcggcggca tcaacgtcgc ccgcgccatt gcccatcttg gaggcagtgc
      241 cacagcgatc ttcccggcgg gtggcgcgac cggcgaacac ctggtttcac tgttggcgga
      301 tgaaaatgtc cccgtcgcta ctgtagaagc caaagactgg acccggcaga atttacatgt
      361 acatgtggaa gcaagcggtg agcagtatcg ttttgttatg ccaggcgcgg cattaaatga
      421 agatgagttt cgccagcttg aagagcaagt tctggaaatt gaatccgggg ccatcctggt
      481 cataagcgga agcctgccgc caggtgtgaa gctggaaaaa ttaacccaac tgatttccgc
      541 ttcgcaaaaa caagggatcc gctgcatcgt cgacagttct ggcgaagcgt taagtgcagc
      601 actggcaatt ggtaacatcg agttggttaa gcctaaccaa aaagaactca gtgcgctggt
      661 gaatcgcgaa ctcacccagc cggacgatgt ccgcaaagcc gcgcaggaaa tcgttaatag
      721 cggcaaggcc aaacgggttg tcgtttccct tggtccacaa ggagcgctgg gtgttgatag
      781 tgaaaactgc attcaggtgg tgccaccacc ggtgaaaagc cagagtaccg ttggcgctgg
      841 tgacagcatg gtcggcgcga tgacactgaa actggcagaa aatgcctctc ttgaagagat
      901 ggtccgtttt ggcgtagctg cggggagtgc agccacactc aatcagggaa cacgtctgtg
      961 ctcccatgac gatacgcaaa aaatttacgc ttacctttcc cgctaacaaa aacattcccc
     1021 cagcattggg ggaatcacca ccaacctgtc ggcaacgcgt ttctccgact ttgctcaaaa
     1081 gtcatgtgat aacaaagggg tgaactatgg ccagtggcga tcttgtccgt tatgtcataa
     1141 ccgtcatgtt gcatgaggat acattgactg aaattaacga gttgaataat tacctgactc
     1201 gcgacggttt tttgctcacc atgacggatg atgagggaaa tatccatgag ctggggacta
     1261 acacttttgg acttatcagt acccaaagtg aagaagaaat tagagaactg gtttcggggc
     1321 ttacccaaag tgcaaccggc aaagatcctg aaatcaccat cacgacttgg gaggaatgga
     1381 atagcaacag aaaataaatg gtttttgggc aataatcagt ctgtggtgtg cgttagctcg
     1441 tgtttttaca ccgcattctt gcgctaacct tatgatctgg cagacaacat gggagagaca
     1501 tcatgtggca ggcaatcagt cgtcttttga gcgagcagtt aggtgaaggc gaaatcgaac
     1561 tgcgtaatga actgcctggc ggagaagtcc atgccgcatg gcatttgcgc tatgcaggac
     1621 atgacttttt cgtcaaatgt gatgaaaggg aactgcttcc cggttttacc gccgaagccg
     1681 accaactgga gttactgtcg cgtagtaaaa ccgtcaccgt gcctaaggtt tgggcagtag
     1741 gcgctgaccg tgactacagt tttctggtga tggattatct cccacctcgt ccgctggatg
     1801 cgcatagcgc atttattctt ggtcagcaaa ttgcgcgttt acatcaatgg agtgaccaac
     1861 cacaatttgg cctcgatttt gataactcgc tctccacaac tccacagccc aacacctggc
     1921 aacgtcgctg gtcaacgttt tttgctgaac aacggattgg ctggcagttg gaactggcag
     1981 cagagaaagg gatcgctttc ggcaatatcg acgccatcgt cgagcatatt cagcagcgtc
     2041 tggcctcgca tcaactgcag ccttctctgt tgcacggcga tttatggtcc ggcaactgtg
     2101 cgctgggtcc ggatggcccg tacattttcg accctgcctg ctactggggt gaccgagagt
     2161 gcgacctggc gatgttaccg ctgcatactg aacagccgcc acaaatctat gacggctatc
     2221 agtcagtatc cccgctacct gccgatttcc ttgaacgtca accggtttac caactctaca
     2281 cgctgttaaa tcgtgcaaga ttatttggcg gtcagcattt ggttattgct cagcagtcat
     2341 tggatagatt attagcagca tgatatgggt tgaggataat ggccgctccg tgcggccttt
     2401 tgattaaata aacccaagca gagaaaagaa cacataacct gcagcaatac aaataaccgg
     2461 caggatatat agtgagaaaa actgcaggaa aatagtatgg tgcggaacaa caatacggga
     2521 ctcaatttgt tcacgcgtta gcccctcttc ccctttggct ttttccagaa tgagttgatc
     2581 ttcaacacct tcccgcaaga agcgcgcctg gcgactcatc cgggcaccgg aatcctgcaa
     2641 cgccagtccg acaaaaatca ggatgaaaat cacccagaac ataacgttca cgccaccatt
     2701 aaaattaggg gtcggggagt tataccagaa cagattcaaa aaaggcgtat tcgcctgcat
     2761 catatcgatc atgacatgag taaagtcgag catgaccgca ttaatgcctt cctggttttc
     2821 ctgccgggta ttcataaatt tcagcaaaga aatcaatgtg gagatcaatg caggtataaa
     2881 aatcacccaa cccaaaatcc ttttcaaaac agcaatgcgt ccagcttgtt gatacgtcat
     2941 gagttctcct tgattaagac gcgtcgtttc acttagttta cctgtagata tctgttttcg
     3001 cccattcttt aaaggcgata tgataggcgc ttaatcataa gcacggctta ataccttaca
     3061 cataatgctc taaaggagag gttgtaatgt caaccccgcg tcagattctt gctgcaattt
     3121 ttgatatgga tggattactt atcgactcag aacctttatg ggatcgagcc gaactggatg
     3181 tgatggcaag cctgggggtg gatatctccc gtcgtaacga gctgccggac accttaggtt
     3241 tacgcatcga tatggtggtc gatctttggt acgcccggca accgtggaat gggccaagcc
     3301 gtcaggaagt agtagaacgg gttattgccc gtgccatttc actggttgaa gagacacgtc
     3361 cattattacc aggcgtgcgc gaagctgttg cgttatgcaa agaacaaggt ttattggtgg
     3421 gactggcctc cgcgtcacca ctacatatgc tggaaaaagt gttgaccatg tttgacttac
     3481 gcgacagttt cgatgccctc gcctcggctg aaaaactgcc ttacagcaag ccgcatccgc
     3541 aagtatatct cgactgcgca gcaaaactgg gcgttgaccc actgacctgc gtggcgctgg
     3601 aagactcggt aaatggcatg atcgcttcta aagcagcccg catgcgttcc atcgtcgttc
     3661 ctgcgccaga agcgcaaaat gatccacgtt ttgtattagc aaacgtcaaa ctctcatcgc
     3721 tgacagaact caccgcaaaa gaccttctcg gttaatgacc aggggcagtg atcacgctgc
     3781 ccctggttct ttatctgaat ttgcattcaa ctgacggatt aatcgtcaat ttaagagaaa
     3841 gagttacacc gtcaccactt ccgtgcactg tataaaaatc ctatactgta cgtatcgaca
     3901 gtttagtgag ttttatcatg acggcggaag gtcaccttct cttttctatt gcttgtgcgg
     3961 tatttgccaa aaatgccgag ctgacgcccg tgctggcaca gggtgactgg tggcatattg
     4021 tcccttccgc aatcctgacg tgtttgttac cggacatcga tcacccaaag tcgtttcttg
     4081 ggcagcgatt aaaatggata tcaaaaccga tcgcccgcgc ttttgggcat cgtggtttta
     4141 cccacagtct gctggcggta tttgcgctgc tggcaacctt ttaccttaag gttccggaag
     4201 gctggttcat tccggctgat gcgctacaag gaatggtgct gggttatttg agccacatac
     4261 ttgccgatat gctgacaccc gccggtgttc ccctgctctg gccatgccgc tggcgtttcc
     4321 gcttgcctat cctggttccc caaaagggca accaactgga acgttttatc tgcatggcat
     4381 tatttgtctg gtcggtatgg atgccccatt cattacccga gaacagcgct gttcgttggt
     4441 catcgcaaat gatcaatacc ttgcagatcc agtttcatcg gcttattaag catcaggttg
     4501 aatactaaaa aggcaaaaat cacctttctg gaataagcaa ttccatttga atataagagc
     4561 cagctcacag ttctgttaat cttgcgccaa cactatgact gctacgcagt gatagaaata
     4621 ataagatcag gagaacgggg atgaactttc cattaattgc gaacatcgtg gtgttcgttg
     4681 tactgctgtt tgcgctggct cagacccgcc ataaacagtg gagtctggcg aaaaaagtgc
     4741 tggtgggtct ggtgatgggt gtggtttttg gccttgccct gcataccatt tatggttctg
     4801 acagccaggt acttaaagat tctgtacagt ggtttaacat cgttggtaac ggctatgttc
     4861 aactgctgca aatgatcgtt atgccgttag tcttcgcctc tattctgagc gcggttgccc
     4921 gtctgcataa cgcatctcag ttaggcaaaa tcagttttct gaccatcggt acgcttttgt
     4981 ttaccacgct gattgcggcg ctggtcggtg tgctggtcac caacctgttt ggtttgacgg
     5041 ctgaaggtct ggttcagggt ggtgcagaaa ctgcacgtct gaacgccatt gaaagtaact
     5101 atgttggtaa agtctctgat ctgagcgttc cgcagctggt cttgtccttt atcccgaaaa
     5161 acccgtttgc cgatcttacc ggagccaatc cgacgtcaat tatcagcgtg gtaatttttg
     5221 ccgcattcct cggcgtagct gcattgaaac tgctgaagga tgatgcgccg aaaggtgaac
     5281 gcgtcttagc cgctatcgat accctacaaa gctgggtgat gaaactggtt cgccttgtca
     5341 tgcagttgac cccttacggc gttctggctc taatgaccaa agtggttgca ggttctaact
     5401 tgcaagacat catcaaactg ggaagtttcg ttgtcgcgtc ctacctcggt ctgctgatta
     5461 tgtttgcagt gcatggcatt ctgctgggca ttaatggcgt gagtccgctg aagtacttcc
     5521 gtaaggtatg gcctgtgctg acgtttgcct ttaccagccg ttccagtgct gcgtctatcc
     5581 cactgaatgt ggaagcacaa acgcgtcgtc tgggcgttcc tgaatccatc gccagtttcg
     5641 ccgcctcttt cggtgcaacc attggtcaga acggctgcgc cggtttgtat ccggcaatgc
     5701 tggcggtgat ggttgcgcct acggttggca ttaacccgct ggacccgatg tggattgcga
     5761 cgctggtcgg tattgttacc gttagttccg caggcgttgc cggtgtcggt ggtggtgcaa
     5821 ctttcgccgc actgattgtg ctgcctgcga tgggcctgcc agtaaccctg gtggcgctgt
     5881 taatctccgt tgaaccgctt atcgacatgg gccgtacggc gctaaacgtt agtggctcga
     5941 tgacagctgg cacgctgacc agccagtggc tgaagcaaac cgataaagcc attctggata
     6001 gcgaagacga cgccgaactg gcacaccgtt aattcttatg ctggcaacgg tccgttttgt
     6061 ataggggccg ttgctttact ttttaaatgt tccatcgcgg cgatgcatac tgactcatat
     6121 ttaaacagat cgtcagcgaa aaaaatgtcc aacttatcaa tacattcctg gctcaatgaa
     6181 tgtgcctgta gtgagaagct taaaacctca tacccgagac gttttctaag tgaactatct
     6241 ggaatactca taatatcttc aagcacacca cttcccattc ccggaagatt tagtggatta
     6301 aaaagaagat tgatatt
//
LOCUS       DAZPEP010000204         6302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZPEP010000204 DAZPEP010000000
VERSION     DAZPEP010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.206"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..273)
                     /locus_tag="ACHUT9_003929"
     CDS             complement(<1..273)
                     /locus_tag="ACHUT9_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350297.1"
                     /translation="MTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNAHILE
                     HGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGECFKKEYSIV"
     gene            complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHUT9_003930"
     CDS             complement(332..4189)
                     /gene="sigA"
                     /locus_tag="ACHUT9_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFR1350298.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4551..5555)
                     /locus_tag="ACHUT9_003931"
                     /pseudo
     CDS             complement(4551..5555)
                     /locus_tag="ACHUT9_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5540..5917
                     /locus_tag="ACHUT9_003932"
                     /pseudo
     CDS             5540..5917
                     /locus_tag="ACHUT9_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5956..>6302)
                     /locus_tag="ACHUT9_003933"
     CDS             complement(5956..>6302)
                     /locus_tag="ACHUT9_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1350299.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1571 a         1408 c         1410 g         1913 t
ORIGIN      
        1 gacaatgctg tattcttttt taaagcactc accagaaacg gtgattgtaa tgataatggt
       61 cttctcgatc gagatgtaaa tacccgtttc gttatactgt ccgtatcgga accatgagaa
      121 ctcagttgca ttccagccat gctccagaat atgtgcattt ttttgcctga tagtttcttt
      181 ttttattcgc tcatgtttaa ggcataacag gcagataaat aatgtcagaa aaagaaagct
      241 aataatgaga gcaagagcga tatagtctgt cacagggtaa tacatattct ctacggtaga
      301 agaagggccg caaacgcggc ccgggctgtt atcagaaaga gtaacggaag ttggcgttga
      361 tcgcgttatc cacgttgtat ttaccaaatg ccgatttctc aaactcaaga ccgaagcgaa
      421 gattatcgcg aatttcggcg ttgagaccaa cattcatgag aatacgaccg tctttttcac
      481 ctttgatacg tttctcaccg gacgcatcac gcagtacggt ttcaccgttg gcaaacaggt
      541 caaactggta gccaaggccg gcgcgggctg tgactttcca gtccttaccg gagaaggatt
      601 tacccacatc aacaccggta cgcccaatca gcggattaaa gtccttatcc ttcatggtca
      661 cgctcattcc gcgatcctgc cagtcgaacc gtttacctga tacggcccca taaaccagtt
      721 ctgcctgcgg ctcaatccag gagtcttctg ttacatgata gcggtagcct gcttcagcac
      781 ccacatacaa ggaatgagaa ctgtagtctt ttgttccgag tccagcaaag gtcgccgagt
      841 actcattatc atggtgaaca tacttactaa tcaggtcgat ataggcacca gagtcaaata
      901 tagcggaagc atacagacct gccccgacgg acttcgtctt gccactgaat gcattactgc
      961 tggcatgact gtcggtatac gtcatagtca gaccagtgaa aaggtcaagt ccatccagct
     1021 catgtttttt atccacacca atctgcacat gggtgtagtt gtcactgtat ccactagaag
     1081 ctgaacctgc tccgctcatg atgcgggccc atgcaccggc ctcgccattg gtgtcacgca
     1141 gatcccccat tcgtttattc aggttgttga cttctgtcag gaaggctttg taattaccgg
     1201 acatatacga ttttgctgca ccggaagatg ctgtattttc agccacctta tatccggtca
     1261 gggtccacgt ggtatgttcc tgatcctctt tcgtgctgat cacaggagat atgttgctga
     1321 aacctatggc ctgagtactg gctttaaata tctctttatt gcttcctttt ggggctgtta
     1381 tcaggtcaac gtttaatcct gagttattat ttcccacttt ctgtatatcg accgacagac
     1441 tgttattgct tccggttaac tgatttttaa caatgagttt atccgattga tttacagact
     1501 ggctcagatc agcccgcata atcacagcag tattagtggc atccagtgtg tttactgtca
     1561 cagtatggaa acccgaattc tctatgttac taaacaacaa aagactgtcg cgggttttta
     1621 atgattgcag ggctgactgt ccgtttactc tccataaggt gttatacatg ctggctgagg
     1681 cagcaggagc atcaatacct ccggtataag aagtatcaaa gccacccagc aaacctgtag
     1741 ccagcggtgt gtgtgtggtt ttgtccaggt tatcttcttt atactggtca acaccaaaca
     1801 gaatagatgc ttttttgtct gattttatat taccattgac ccaggcataa ttttgagcct
     1861 ttaacgtggc accatcttct gtcatattat atccatctga catatagatt gccgggctgt
     1921 atttattatt ttctgtaggg gtgcctgtta atgtcagggt tccggccagt tcaatagctt
     1981 tatctacata catttttccc tgggcggtaa cagtagctcc tttgttgata atcaggtctg
     2041 tattaattaa ggataatgca ccagtcatag taacagttga tttatcacca acagttattt
     2101 tgctgttttg agcactcaga ctgccgttga aactactttc gcctgctgaa tccccggagc
     2161 gaagctgttg tctgaatcca aaaccttcgc cagtaatgtt tcttcctgag aacatgtcaa
     2221 tgtatgcagt tttatttccc agaacaattt gagagttttt agcctctatg tgtcctttta
     2281 cattagcatc ccggcctata ctcagggttg cgttttctaa atttaatttc cggaaattaa
     2341 atttacgact ttcccagtcg ggctgggaaa aagaggctat ctgattgttg gacttatatt
     2401 ccgtattgta acgtttattt acagtgcttt cctggtcctt tatggcagca gaatagtctt
     2461 tttgacataa aaaaggaatt ggacagttgt tattgccttc tttaaatatg gcatgttctg
     2521 tcgcatgtcc ttgtaatgtt aactgtgcat tccggacaga gatatcgttt ttgatatcga
     2581 ctgagccatc aaagatcaga ttggcattat tgttatgttg ctgggtagta ttgataatat
     2641 gatttatgct tatattacct gatacattcc catggtacat atatgcatca gtattagtaa
     2701 gtgataatgt tgattgcttc acgtttgagt tcgttacggt tgttcccgca tctgttgctg
     2761 cgattttctg aaatgactgg tcatagccgt ttagatccag tgttccgcca tttctggtaa
     2821 aatagatttc gccattaccg ctttcgctta aagcgtcttt ggcatttatt tttaccgtac
     2881 ctttacctcc ggccatgtaa attttattga aggctttttc agcactaagg atgacagtcc
     2941 cattacctat tttaaggtta tttccctgtg ctatttttac atccagagta ccactaccta
     3001 ttttatgcag attatctccg gcaacgccct taacattcca ttcaacggtg gtattattat
     3061 caatatctat cccggcacct ttataagact tatcttttcc gtacacttta tatgtctggt
     3121 tcttatcgaa tataagcccg ccaatgccaa gatcggtatc ctgcttgagc gttaattccc
     3181 ctcctcctga gacagacagg tccttatctt ttataatcga tgataactct tgttttttac
     3241 catccagctg catagtgttt gtgaaattct tttttaaatt gtttaccaga atattattaa
     3301 ataacgagta gtttgtccag gctatattgg tactactgag aaaaccaact gtactggtaa
     3361 caccatggaa aacccatttt tgttgttctt tatcatatag ataagaacca gaaccactat
     3421 cgccaaaaat tggtgagttt ttaaaagggg ttctgttggt ataaatgtaa ctcttgtttt
     3481 tccagtccag attaaaaaga cttgcgctta acaagtcggg attatagttt tgtccaaaca
     3541 gatattgttt gccgtttaat atcgttgagg ttgttccggc acctgctctg aagccaatta
     3601 tttgttcctt accgttgtag tttacgccat atcttttcat cgcatcttcg ggagaaagtg
     3661 agaagtcggc actctccgta acccctgttg tttccacgac gaatttattc agacgatgaa
     3721 ccgaaaagtc gccagatgat actctgttac ttgctttata tgctgtctga tcccatgact
     3781 gtgttgttat tgcatgatgc tgtgtaccgt tatgagtcgc agtaacactg tacgcacctc
     3841 caattgatgt tgttgcacct ttgttggaaa cagcagaaaa atcaggtaca ggtagttctg
     3901 gaaaatgaaa tatctttcca tctttcatca ttaattgaac atttgttgca cccggtctga
     3961 actctcctct gttttgagca aggtcaagat aatcgcgggt ccatatatta tttatatcca
     4021 gcattgctgc aaatgctgtt tcgccataac ttccatatat tgaggataaa ataataccga
     4081 ggataacttt ctttcttgat gtaccgacac taactttccg ggtcagttca gaaacagcaa
     4141 ctaatccacc tgtaatatga ctatatttca gtgaataaat tttattcata cgtgaactcc
     4201 gatatttctg agttgtggtt atgggatagc atttaattac aacggggaac tatcagtcat
     4261 ggtacgtaac tgataagggt ataatgataa tcactgtcag ttatatttgt atgacagatg
     4321 taacaaaatg aaaaaaaaag ataagcacaa gtgattgatt ggtcagatat tgatcacttg
     4381 gtgtttgtat tttgaacttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     4441 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     4501 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     4561 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     4621 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     4681 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     4741 gcgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     4801 tccacatcaa atgcggtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     4861 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacggggg
     4921 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     4981 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5041 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5101 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5161 tcagtgatca gtgccgcgcg ggcaaaggcc tgtgatgtca ggcggctttt gtccacctcc
     5221 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5281 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     5341 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     5401 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     5461 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     5521 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     5581 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     5641 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     5701 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     5761 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     5821 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     5881 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     5941 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6001 aatatcccac ataaatccag ttaactcacg agccaccgcc gtaatggcta cttgtgattt
     6061 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6121 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6181 ttttgcaggt gtacgataag accaagcggc ttcgtaaagc agacgtctca gttcgctgtt
     6241 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6301 ca
//
LOCUS       DAZPEP010000205         6105 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZPEP010000205 DAZPEP010000000
VERSION     DAZPEP010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.207"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHUT9_003934"
     CDS             complement(<1..264)
                     /locus_tag="ACHUT9_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFR1350300.1"
                     /translation="MLKQRRNFCTGTERHANRFTTSASRSNIRYSLSDTHATPDGYPV
                     KQIGEHAWLIEKAGIVIHKCPRNPFTGNRIFALSCGDNQFGQDF"
     gene            complement(279..557)
                     /locus_tag="ACHUT9_003935"
     CDS             complement(279..557)
                     /locus_tag="ACHUT9_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1350301.1"
                     /translation="MAKVLNTYEQADFERLAAFYPYRNEHGLPVLEESLEDYAKRTNQ
                     SILAVRRQADRSVIPVTQEGRNTKRKVNLFAIFLKTIRNAEKYVQMTK"
     gene            653..964
                     /locus_tag="ACHUT9_003936"
     CDS             653..964
                     /locus_tag="ACHUT9_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1350302.1"
                     /translation="MSIEISKKLKAIRESEGLSQAKFADSIGIAVGTVKQYETGIRGV
                     GTEVLLKITMHPEFKKYTTWLMSNETNEAAGQISPSLSPDGPENTSSSQKSRKTGTQP
                     G"
     gene            1053..1991
                     /locus_tag="ACHUT9_003937"
     CDS             1053..1991
                     /locus_tag="ACHUT9_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1350303.1"
                     /translation="MRPEGRKGKRIRKKFKTKSDAVLYERWVLAQQHNNEWKGNSIDR
                     RPLSVLIDLWWKYHGQLMKSGHNTRLKLLRLSEAMDDPCVHKLNTTMLTELRVSRIEQ
                     GIQPSTINREIGALSAMFTALISSGHFLNDNPVQGLKGMKVNEREMGYLSKSECVQLL
                     DALAENPDERLAVEILLSTGARWGEVAALEQRRVLHCRITFSKTKNSKNRTVPISESL
                     FEKIKKRGGKLVFPTLDYSLVRDVIKTVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGM"
     gene            1994..2494
                     /locus_tag="ACHUT9_003938"
     CDS             1994..2494
                     /locus_tag="ACHUT9_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350304.1"
                     /translation="MEIKKPTKKELYDYLLSKYIEDKCKEEADEINKKSMSRVKKHKE
                     RLMEITPEIFFRFLSEKGVSSVCPSCGSSRLSVPESMDLCWDKNKKPENFNNLPLEEQ
                     SELIKENIKHYVSYAFLGDVKSIPDMRKTYYTLHCLNCGYLSLYRTSAVLKWLEKDKA
                     QDGDNG"
     gene            2595..3032
                     /locus_tag="ACHUT9_003939"
     CDS             2595..3032
                     /locus_tag="ACHUT9_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1640 domain-containing protein"
                     /protein_id="HFR1350305.1"
                     /translation="MSDKLERRIERLEGDLSLTRNDLATLAERTTNLSTKADVGEVKG
                     ELKADIAHLKGDLECDIANLKGELKSDTANLKEQLKSDINSLKGELTEAMDKRFDKIM
                     DEMNRRFDKVDDNTKWRWSGIIVPVCTTIFTAAVAVFVAKFVG"
     gene            3225..4205
                     /gene="btuC"
                     /locus_tag="ACHUT9_003940"
     CDS             3225..4205
                     /gene="btuC"
                     /locus_tag="ACHUT9_003940"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFR1350306.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            4268..4819
                     /gene="btuE"
                     /locus_tag="ACHUT9_003941"
     CDS             4268..4819
                     /gene="btuE"
                     /locus_tag="ACHUT9_003941"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFR1350307.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            4819..5568
                     /gene="btuD"
                     /locus_tag="ACHUT9_003942"
     CDS             4819..5568
                     /gene="btuD"
                     /locus_tag="ACHUT9_003942"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFR1350308.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            5646..>6105
                     /locus_tag="ACHUT9_003943"
     CDS             5646..>6105
                     /locus_tag="ACHUT9_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1350309.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         1527 a         1331 c         1645 g         1602 t
ORIGIN      
        1 gaaatcctgc ccgaactgat tgtcgccaca gctcagtgca aaaatgcggt ttccggtaaa
       61 cggattgcgt gggcatttgt ggatcacgat tccagctttc tcaatcagcc aggcgtgctc
      121 gccgatttgt tttactgggt agccatccgg cgttgcgtgt gtatcactca ggctgtagcg
      181 gatgttgctg cgtgatgcac tggtagtgaa acggttagcg tggcgttctg ttccggtaca
      241 aaaattacgg cgttgcttca gcataaaatg acacctcgtt attttgtcat ctgcacgtat
      301 ttctctgcgt ttctgatggt tttcaggaaa attgcgaaga gatttacttt gcgttttgtg
      361 tttcttcctt cttgagtaac gggaattacc gatctatcag cctgccttct tacagcgaga
      421 atgctttgat ttgtgcgttt cgcataatcc tctaggcttt cttcaagtac cggtagccca
      481 tgttcattac ggtatgggta gaacgccgcc aaacgctcaa aatccgcttg ttcgtatgtg
      541 ttaaggactt ttgccatggt gtgataacct attcaatctg gtgctatttg tggctctttg
      601 tagcgtcaag tggtactcaa ctgataacca atatagtatt caggtgcaca ccatgtcaat
      661 agagatatca aagaagctaa aagcaattcg agaatctgag gggcttagtc aggcaaagtt
      721 cgcggattca ataggtattg cggttggtac ggttaagcaa tacgagactg gtattcgagg
      781 tgtgggaacg gaggttttac tgaaaatcac aatgcacccg gaatttaaaa aatacactac
      841 gtggttgatg agtaacgaaa caaatgaggc tgctgggcag atcagtcctt ctctctcccc
      901 tgatgggcca gaaaacacat cgtcttctca aaaatcccgc aagactggca cacagcccgg
      961 ctaatcatgg aacgctgggg gcatggtggt cttgtaacgc tggggcttca cgaatgagca
     1021 taaaatcaat tccgggaggg tatcttcttg acatgcgtcc tgaggggcgt aaaggcaaac
     1081 gcattcgcaa aaaatttaaa acgaaatcgg atgcagtttt atatgagcgg tgggtgctgg
     1141 cgcaacagca taacaatgag tggaaaggaa actccattga tcgccgtccg ctgtcagtgc
     1201 ttattgactt gtggtggaaa taccacggcc agctaatgaa gtcagggcat aacacgcgcc
     1261 ttaaattgct gcgcttgagt gaggcaatgg atgacccgtg cgtgcataaa cttaatacaa
     1321 cgatgctcac cgagctacgt gtgtccagga tagagcaggg gatacagccc agcaccataa
     1381 atcgagagat tggggcgtta agcgcgatgt ttaccgcact catctcatcc ggccattttc
     1441 ttaacgataa ccccgttcaa ggccttaaag gaatgaaggt taacgagcgt gaaatgggat
     1501 atctgagtaa gtctgaatgt gttcagttgc tggatgcact ggctgaaaat cccgatgaac
     1561 ggctggccgt cgaaatcctt ctgtcgaccg gggcgcgatg gggcgaggta gcggcactgg
     1621 agcagcgccg tgttcttcat tgtcgaatca ctttttcaaa aacgaagaac agcaaaaacc
     1681 gtaccgttcc tatttctgaa agcctgtttg aaaagatcaa aaaacggggc gggaaactgg
     1741 tgtttccgac gctggattat tcattggttc gcgatgtcat caaaacggtc gcacctgatg
     1801 ttcctgacgg ccaggctgtt catgcgctgc gccacacctt cgccagtcat ttcatgatga
     1861 acggcggcaa tattctgacg ctccagaaaa ttctggggca cgcaaagatt cagacaacga
     1921 tgatttatgc ccatcttgcg ccggattact tgcaggatgc ggtgaggttt aatcccttgg
     1981 gaggaatgta actatggaaa taaaaaaacc tacaaaaaaa gagttatatg actatttact
     2041 atcgaaatat atagaagata aatgcaaaga agaagctgat gaaatcaata agaagtcgat
     2101 gagtcgtgtc aaaaaacata aagagcggct gatggagatt acgccagaaa tcttcttccg
     2161 tttcttgtct gagaaggggg tctccagtgt ctgcccttca tgtggatcgt cgcgattatc
     2221 tgttccagaa agtatggatc tttgctggga taaaaataag aaaccagaaa attttaacaa
     2281 tctacccctg gaggaacaga gtgagttaat taaagagaac ataaagcatt atgtatctta
     2341 tgcttttttg ggtgatgtta agagcatacc tgatatgcgc aaaacttatt acacgctgca
     2401 ttgcctgaat tgtggttacc tgagccttta tcgtacgtct gcggtgctga agtggttgga
     2461 gaaagacaaa gcgcaggatg gtgataatgg gtaatgtagc aagaaatctt ttcggtaatt
     2521 cagcaggtag tgtgtcgcat tctgagcgtg atgtgcttta tcatggtggc gatggcggca
     2581 gtagtggagg cggaatgtca gataaacttg aaaggcgaat tgagcggctc gaaggtgatt
     2641 tatcgctaac aagaaacgac cttgcgacgc ttgctgaacg cactacaaac ctctcaacta
     2701 aagccgatgt tggtgaggtg aaaggtgaac tcaaagcaga catagcacat ctgaaaggtg
     2761 atcttgaatg cgatattgcg aatctgaaag gtgagcttaa atctgataca gctaacctga
     2821 aagagcagct caaatcagac attaacagcc tgaagggtga gcttaccgaa gcgatggata
     2881 aacgctttga caagattatg gatgagatga atcggcggtt tgacaaggtt gatgataata
     2941 cgaagtggcg ttggagtggc attattgtgc cagtgtgcac aaccattttc acagcggcgg
     3001 ttgctgtatt tgttgctaaa tttgttggct gatgatccac aaattgacca catctctgtt
     3061 atttgttgtg gttggctgtg tttttgtgtg tctgtaagtc tttgataatt acctaactta
     3121 ttgatttttg tttgtgttta tggccgctct gcggcctttt ttcttttcac tgtcgaagag
     3181 tcaccgtaaa atcaacgcca tgacacttca gcagaacgga taccatgctg acacttgccc
     3241 gccaacaaca gcgacaaaat attcgctggt tattatgcct gtcagttttg atgctgctgg
     3301 cgcttctctt aagcctttgc gccggtgaac aatggatttc gccaggtgac tggtttactc
     3361 ctcgtggcga actgttcgtc tggcaaattc gcctgccacg tacgctggct gtattgctgg
     3421 ttggtgcggc gctggctata tccggcgctg taatgcaggc gttgtttgaa aatcctctgg
     3481 cagaacctgg actacttggc gtctctaacg gcgcaggcgt ggggcttatc gccgcggtat
     3541 tgcttgggca agggcaactc cccaactggg cgctagggct gtgtgcgatt gctggcgcgc
     3601 ttatcatcac tttaatactc ttacgtttcg cccgtcgtca tctttcgacc agtcggttat
     3661 tgctggctgg cgttgcatta gggattatct gtagcgcact aatgacgtgg gctatctact
     3721 tttccacctc tgttgattta cgtcagctga tgtactggat gatgggcggt tttggcggcg
     3781 tagactggcg gcaaagctgg ctgatgctgg cattgatccc cgtgttgttg tggatctgtt
     3841 gtcagtccag gccgatgaat atgttagcac ttggcgagat ctcggcgcgg caactgggtt
     3901 tacccctgtg gttctggcgc aatgtgctgg tggcagcgac cggctggatg gttggcgtca
     3961 gtgtggcgct ggcgggtgct atcggcttta ttggtctggt gatcccacat attcttcggt
     4021 tgtgtggttt aaccgatcat cgcgtattac ttcccggctg cgcgctggca ggggcgagcg
     4081 cagtgctgct ggccgatatt gtagcgcgcc tggcattagc tgccgcagag ctgcctattg
     4141 gcgtggtcac cgcaacgttg ggtgcgccgg tgtttatctg gttattgtta aaagcaggac
     4201 gttagccgca aaaagacggt ctatgattaa aagctaagat ttttactgac cacacccagg
     4261 agaaacgatg caagattcca ttctgacgac cgtagtgaaa gatatcgacg gtgaagtgac
     4321 cacgctggag aagtacgccg gtaatgtgct gttgattgtc aatgtcgcct caaagtgtgg
     4381 cttaacgccg caatatgagc agttggagaa tattcagaaa gcctgggccg atcgaggttt
     4441 tgtggtgctg ggattcccgt gcaaccagtt tctggaacaa gaaccgggca gcgatgaaga
     4501 gattaaaact tactgtacca ccacatgggg ggtgacgttc ccgatgttca gtaagattga
     4561 agttaacggc gaaggacgcc atccgctgta tcaaaaattg attgccgcag cgccgaccgc
     4621 agtcgcgccg gaagagagcg gattctatgc ccgtatggtc agcaaaggtc gtgcaccgct
     4681 gtaccctgat gatattttat ggaattttga gaaattttta gttggcaggg acggattggt
     4741 catccagcgt ttttccccgg atatgacgcc ggaagacccc attgtgatgg aaagcattaa
     4801 actggcgttg gcaaaataat gtctattgtg atgcagttac aggatgttgc ggaatctacc
     4861 cgcctggggc cactttctgg cgaggttcgg gctggggaga tcctgcacct ggtggggccg
     4921 aatggcgcgg gtaagagtac cttactggca cgaatggccg gaatgaccag cggtaaggga
     4981 agtattcagt tcgcggggca accactggaa gcatggtccg caacaaaact cgcgctgcat
     5041 cgcgcctatc tttcacaaca gcagacgccg ccgtttgcaa tgccggtctg gcactatctg
     5101 acactgcatc agcacgataa aacgcgtacc gaactactga atgatgtcgc aggggcgctg
     5161 gctcttgatg acaaactcgg acgtagcacc aatcaacttt ccggcggtga atggcaacgc
     5221 gtacgtcttg ctgcggtggt gctgcaaatc acaccacaag ccaatcccgc aggccaattg
     5281 ctgcttcttg atgagccgat gaacagtctt gatgttgcgc aacaaagtgc gttagacaaa
     5341 attctgagcg ctctgtgtca gcaaggactg gcgattgtga tgagcagtca cgatctcaac
     5401 cacacattgc gtcatgcgca tcgggcgtgg ttgctaaaag gtggaaaaat gctggccagt
     5461 ggacgcagag aagaggtgct cacgccgcca aatctggcgc aggcctatgg gatgaatttt
     5521 cgccgtctgg atatcgaagg tcacagaatg ctgatttcga ccatctgatt tcattagtat
     5581 cttgaaaatc gggtaattac gacgctaaat taatgcaaga ataaaaaaca gaggaaccgt
     5641 acggaatgcg tttctgcctt attttgatca cagcactgtt tctggccggg tgtagccacc
     5701 ataaagcacc gccgccaaat gccagacttt ctgattcgat taccgttatt gccggtttga
     5761 acgaccagct acaaagctgg catggcacgc cgtatcgtta tggtggcatg acgcggcgcg
     5821 gtgtggactg ttcgggattt gtggttgtaa cgatgcgcga tcgtttcgat ttgcagctgc
     5881 cccgagaaac caaagaacaa gcctctatcg gcacgcaaat tgataaagac gagttgctgc
     5941 ctggtgacct ggtctttttc aaaacgggtt ccggacaaaa tggtttgcat gtaggtattt
     6001 acgataccaa caaccaattt atccacgcct ctaccagcaa gggagtgatg cgttcctcac
     6061 ttgataatgt ctattggcag aaaaatttct ggcaggcgag acgaa
//
LOCUS       DAZPEP010000206         6066 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZPEP010000206 DAZPEP010000000
VERSION     DAZPEP010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.208"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="ACHUT9_003944"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="ACHUT9_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1350310.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUT9_003945"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="ACHUT9_003945"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFR1350311.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="ACHUT9_003946"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="ACHUT9_003946"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFR1350312.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUT9_003947"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="ACHUT9_003947"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFR1350313.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUT9_003948"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="ACHUT9_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFR1350314.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1439 a         1531 c         1646 g         1450 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catatttgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatggc gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaa
//
LOCUS       DAZPEP010000207         5931 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZPEP010000207 DAZPEP010000000
VERSION     DAZPEP010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.209"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            292..368
                     /locus_tag="ACHUT9_003949"
     tRNA            292..368
                     /locus_tag="ACHUT9_003949"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUT9_003950"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="ACHUT9_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFR1350315.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="ACHUT9_003951"
     CDS             complement(1357..2247)
                     /locus_tag="ACHUT9_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFR1350316.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYIRAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUT9_003952"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="ACHUT9_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFR1350317.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHUT9_003953"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="ACHUT9_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HFR1350318.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1346 a         1560 c         1549 g         1476 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg ccctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagcg cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggt gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAZPEP010000208         5912 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZPEP010000208 DAZPEP010000000
VERSION     DAZPEP010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.210"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(38..400)
                     /locus_tag="ACHUT9_003954"
     CDS             complement(38..400)
                     /locus_tag="ACHUT9_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFR1350319.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUT9_003955"
     CDS             937..2850
                     /gene="mtlA"
                     /locus_tag="ACHUT9_003955"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFR1350320.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUT9_003956"
     CDS             3080..4228
                     /gene="mtlD"
                     /locus_tag="ACHUT9_003956"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFR1350321.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUT9_003957"
     CDS             4228..4815
                     /gene="mtlR"
                     /locus_tag="ACHUT9_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFR1350322.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUT9_003958"
     CDS             complement(4827..5036)
                     /gene="yibT"
                     /locus_tag="ACHUT9_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFR1350323.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5321..5683
                     /locus_tag="ACHUT9_003959"
     CDS             5321..5683
                     /locus_tag="ACHUT9_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFR1350324.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1502 a         1456 c         1524 g         1430 t
ORIGIN      
        1 agcaccacgt aagttaaaac aatcaataga tccataatta gatctccggg aacgtggaat
       61 agtcagtctt tttctcggcg gcgagttgat gctctagccg ggaaattcgg tcagaaagtg
      121 cggcgatttc cgggtcagtc gctttctcct gcgacgcaac atgtgactgc atgccccacc
      181 cgcgctccgg ttgatagagc gtcgcccaga tccacaggaa cggccagata acatgcaggg
      241 taaacaggct cacccagccc gccgtatgaa tagcgtcggc atggggatgg ttgcgctttt
      301 tggcaatcag ataagggatg tcatgtatcg cgatgattcc ataaaaaatc accagaaaga
      361 cgaagatcag cactcccaac gcgaaatagt ttagaaacat atctgcctcg gattcacgtt
      421 tatcagtgtt gtttttgggc tggcagccag aagggagtca ggctgatatt ttgacaataa
      481 tccgggttcg cgattctcgc cataacacca aagaataatt tttagaggtg atgagttgct
      541 tagttacata acaattgtat gacgaaggca taacatgctg tagatcacat caggtgaacg
      601 ccgtaagaaa atatcttgtg attcagatca caaagattca acaaaccatc aaaacaaaaa
      661 tgtgacacta ctcacattta aatgccattt ttagcgaaaa tcgccgcctt gttgcttttt
      721 tacacaagcg ttttgtgatg aacgtcacgt taattacctc tctaccccct atatttatgt
      781 gattgatatc acacaaaagg ccgtcgactg gacagttaac cgattcagtg ccagatttcg
      841 cagtatctac aaggtccggc tacctctgcc gccacattaa caaaaaacct cgggcttcca
      901 gcctgcgcga cagcaaacat aagaaggggt gtttttatgt catccgatat taagatcaaa
      961 gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc caaatatcgg cgcgtttatc
     1021 gcgtggggta tcatcaccgc gttatttatt ccaacagggt ggttaccgaa cgagacgctg
     1081 gcgaagctgg tcgggccgat gatcacttat ctcctgccgc tgctgatcgg ttataccggt
     1141 ggtaagctgg taggcggcga acgtggcggc gtagtcggtg ccatcaccac catgggcgtt
     1201 atcgtcggcg cagacatgcc gatgttcctc ggttctatga ttgcaggtcc gctgggcggc
     1261 tggtgcatta agcacttcga ccgctgggta gacggtaaga tcaaatccgg ttttgagatg
     1321 ctggtgaata acttctccgc aggcatcatc gggatgatcc tcgctattct ggcattcctc
     1381 ggcattggcc cgattgttga agccctgtcc aaaatgctgg ctgcgggcgt taacttcatg
     1441 gttgtccatg acatgctgcc gctggcgtct atctttgttg aaccggcgaa aatcctgttc
     1501 ctcaacaacg ccattaacca cggtatcttc tcgccgctgg gtattcagca gtcccatgaa
     1561 ctgggtaaat caatcttctt cctgattgaa gctaacccag gtccaggtat gggcgtgctg
     1621 ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt ctgcgggcgg tgcggcaatc
     1681 atccacttcc tgggtggtat ccacgaaatc tacttcccgt atgtgctgat gaatccgcgt
     1741 ctgatcctcg cagtcatcct cggcggtatg actggcgtgt tcacgctgac tatcctgggc
     1801 ggtggtctgg tttctccggc atctccgggt tctatccttg ctgtactggc gatgacacca
     1861 aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg cgatggctgt ctccttcgtt
     1921 gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag aagatgatat tgaagcagca
     1981 actcgtcgta tgcaggacat gaaagctgag tctaaaggca catctccgct gtctgcgggc
     2041 gatgtgacta acgacctgag ccacgtacgt aaaatcatcg ttgcctgtga cgccggtatg
     2101 ggttccagtg cgatgggcgc aggcgtgctg cgtaagaaaa ttcaggatgc aggtctgtca
     2161 cagatttcgg tcactaacag cgcgatcaac aacctgccgc cagatgtgga cctcgtcatc
     2221 actcaccgtg acctgaccga acgcgctatg cgccaggttc cgcaggcgca gcatatttcg
     2281 ctgaccaact tcctcgacag cggcctgtac accagcctaa ccgaacgtct ggttgctgcc
     2341 cagcgccata ctgaaaacga agtgaaagtg aaagacagtc tgaaagacag ctttgacgat
     2401 tccagtgcta acctgttcaa actgggcgcg gagaacatct tcctcggtcg caaagcagca
     2461 accaaagaag aagcgattcg ttttgctggc gagcagctgg tgaaaggcgg ttacgttgag
     2521 ccggaatacg ttcaggcgat gctggatcgt gaaaaactga ccccgactta tctgggtgag
     2581 tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc gcgtactgaa aacgggcgtg
     2641 gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag aagaagatga cattgcccgt
     2701 ctggtgattg gtattgcagc ccgtaacaac gagcacattc aggttatcac cagcctgacc
     2761 aatgcactgg atgatgagtc cgtcatcgag cgtctggcac acaccaccag cgtggatgaa
     2821 gtgctggaac tgttggcagg tcgtaagtaa tccaatccca ccctctccac atggagaagg
     2881 tggggttaat tgcctgatgc gctacgctta tcaggcctac aggatgcatc acgatttgtt
     2941 gaatttgcac gttcttgtag gccggataag gcgcttacgc cgcatccggc gctgcccctc
     3001 tcctcacgga gagggtttgg gtgagggaaa agcctcaccc cagccctctc gggtaaaaac
     3061 attgatgaag gttaatacta tgaaagcatt acattttggc gcaggtaata tcggtcgtgg
     3121 ctttatcggt aaactgctgg cagacgcggg tatccaactg acgtttgccg atgtcaatca
     3181 ggtggtactt gatgccctga atgcccgtca tagctatcag gttcatgtgg ttggtgaaac
     3241 cgagcaggta gataccgttt ccggcgtcaa tgctgtcagc agcattggtg atgatgtcgt
     3301 tgatctgatt gctcaggtcg atttagtcac taccgccgtt ggcccggttg tgctggaacg
     3361 tattgctccg gctatcgcca aagggctggt gaaacgcaaa gaacaaggta atgaatcccc
     3421 gctgaacatc atcgcctgtg aaaacatggt acgcggtacc acgcagctga aaggccatgt
     3481 gatgaacgcc ctgccggaag acgccaaagc gtgggtagaa gaacacgttg gctttgtcga
     3541 ttccgccgtt gaccgcatcg taccgccttc ggcttcggca actaacgatc cgctggaagt
     3601 gacggtagaa actttcagcg aatggattgt cgataaaacg cagttcaaag gcgcactgcc
     3661 gaacatccca ggcatggagt taaccgacaa cctgatggca tttgtcgaac gtaaactctt
     3721 caccctgaac acgggtcatg ctataaccgc gtacctcgga aaactggccg gtcatcagac
     3781 cattcgtgac gctattctcg acgagaaaat ccgcgcggtg gtaaaaggtg cgatggaaga
     3841 aagtggtgcg gtactgatca agcgctacgg ctttgacgca gacaaacatg cggcgtacat
     3901 ccagaaaatc ctcggtcgtt ttgagaaccc gtatctgaaa gatgatgtag agcgcgtagg
     3961 ccgtcagccg ctgcgtaaac tgagtgctgg cgaccgtctg atcaagccat tgctcggtac
     4021 gctggaatac agccttccgc acaagaatct gattcagggg attgctggtg caatgcactt
     4081 ccgcagtgaa gatgatccgc aggctcagga actggcagca ctgatcgctg acaaaggtcc
     4141 gcaggcggcg ctggcacaga tttccgatct tgatgccaac agcgaggttg tatccgaggc
     4201 ggtaaccgct tataaagcaa tgcaataatg gtggaccagg cgcaggacac cctgcgcccg
     4261 aataacagat tgtcagatat gcaggcaaca atggaacaaa cccaggcctt tgaaaaccgt
     4321 gtgcttgagc gtctgaatgc tggcaaaacc gtgcgaagct ttctgatcac cgccgtcgag
     4381 cttttgaccg aggcggtaaa tcttctggtg cttcaggtat tccgcaaaga cgattacgcg
     4441 gtgaagtatg ctgtagaacc gttactcgac ggcgacggtc cgctgggcga tctttctgtg
     4501 cgtttaaaac tcatttacgg gttgggcgtc attaaccgcc aggaatacga agatgcggag
     4561 ctgctgatgg cattgcgtga agaactaaat cacgacggca acgagtacgc ctttaccgac
     4621 gacgagatcc ttggaccctt tggtgaactg cactgcgtgg cggcgttacc accgccgcca
     4681 cagtttgaac cagcagactc cagtttgtat gcaatgcaaa ttcagcgcta tcaacaggct
     4741 gtgcgatcaa caatggtcct ttcactgact gagctgattt ccagaatcag cttaaaaaaa
     4801 gcctttcaaa agtaagcaac gtctgcttac tgcccctcta cctgcttcgg ccgataaagc
     4861 cgacgataat actccagacg ttgtagatat aacggcacgc tttcatcggg tattccggac
     4921 ggaatcgcgt tacggggagg aagttttttc agatactccc ggaacgcctg gcttgatgcc
     4981 atgaaatcta cggctttatc gataagaagc ggaacgtttt cacctaattt gcccatgatg
     5041 atatctccgt attaccccgc gccgggaatg cgcggccgcc aattttagtt taggttcccg
     5101 gtaaagatga cattaaggaa aacgtgctga atcctcaata atcatccgct gctgtttatt
     5161 atcttttttc tatagatttc ttaattaatc aataaattgc gtagttttta agcatgttaa
     5221 tgattacgtt acagaactat tgcaatgtca tcagttgtga acaaagcacc tggtcgcgca
     5281 tactaggggc tataaattta tctttatcag aagccatcac atgaaagaag tcgaaaaaaa
     5341 cgaaatcaaa cgtctcagcg atcgtctgga cgccatccgc caccagcagg ccgatctgtc
     5401 gctggttgaa gccgcagaca aatatgccga gctggaaaaa gagaaagcca cgctggaagc
     5461 agaaattgct cgcctgcgtg aagttcatag ccaaaaactg agtaaagaag cacagaagct
     5521 gatgaagatg ccgtttcagc gcgcaattac caaaaaagag caggctgata tgggcaagct
     5581 gaagaaaagt gttcgcggac tggtggttgt gcacccaatg accgcgctgg gccgtgaaat
     5641 gggcctgcag gagatgactg gattctctaa gactgcgttt taagcgcctc agacgactgg
     5701 ttcgatcata tcaacccgca tctggcttgc cttagctggt caatgcgggg actctcctgt
     5761 ctactcgcct aattaccaaa caattcactc cctttacgtc gatttaccgc tggttgatat
     5821 tctgaaaatg catttttacc gaaatagcca cctataaaca ataaaaatat atagaagata
     5881 tcatttatta attaacaagc agttacgtct ct
//
LOCUS       DAZPEP010000209         5901 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZPEP010000209 DAZPEP010000000
VERSION     DAZPEP010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5901
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.211"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1484
                     /gene="tssI"
                     /locus_tag="ACHUT9_003960"
     CDS             <1..1484
                     /gene="tssI"
                     /locus_tag="ACHUT9_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1350325.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASDLHGEQPQAVPGRRGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIE
                     TVGSNQIIKVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRV
                     GLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGT
                     KIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            1552..5547
                     /locus_tag="ACHUT9_003961"
                     /pseudo
     CDS             1552..5547
                     /locus_tag="ACHUT9_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT         1409 a         1499 c         1909 g         1084 t
ORIGIN      
        1 tctgcatcag ccagtttcgc tacagcgcac aaatccgtcc ttcttccgta gtgaccaaag
       61 actacacctt taaacgtccc ggctggccag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacgcagtat gaagtgtatg actaccccgg acgtttcaag ggtgcccacg
      181 ggcagaactt tgcccgctgg cagatggacg gctggcgaaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttcgccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcgagcga tcttcacggc gaacagccgc
      361 aggcggtacc gggacgcagg ggttcaggca ccacgctgga gaaccatttt gcggtaatcc
      421 cggcagacag aacatggcgg ccacagccgt tgatgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggct ttggcaacct ggcgataccg cgtgtgggtc
      661 aggaggtgat tgtggacttc ctcaacggcg acccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggca gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgcgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgctg aaaccattgg caacaatcag atgattgcgg
      961 ttaccaacaa tcagatagag acggtgggcg ttaaccagat agagacggtg ggcagtaacc
     1021 agataattaa agtgggttct gttcaggtgg aaacgattgg acttgttcgt gcgctgacgg
     1081 tgggcgtggc gtatcagacg acggtaggtg gcattatgaa cacctcggtg gcactgatgc
     1141 agtcctcgca gatcggtttg cataaatcgt tgagggtcgg gctgggttat gacgtcaaag
     1201 tcggaaataa cgttaccttc accgttggta aaacgaaaaa ggatgatacc gggcagaccg
     1261 cgatttactc cgccggtgag catctggagc tctgctgtgg taaggcaagg ctggtgctga
     1321 cgaaggacgg acaaattttt ctcaacggca caaaaattca tttgcagggt aaggagcagg
     1381 ttaatggtga ctcactgttg attaactgga actgtgcagc ctcgaaatcc ccaccgaaga
     1441 cccctgatga aaagcaggat acgccggata tgagagagta ctgatcccgt acctgttcgg
     1501 tattcagtaa caaaccagca ttaaaaataa acagaagaag gaatttgaat catgagcgga
     1561 aaaccagcgg cgcgtcaggg agatatgact cagtatggcg gtcccattgt ccagggttcg
     1621 gcaggtgtaa gaattggcgc gcccaccggc gtggcgtgct cggtgtgtcc gggcgggatg
     1681 acttcgggca acccggtaaa tccgctgctg ggggcgaagg tgctgcccgg cgagacggac
     1741 cttgcgctgc ccggcccgct gccgttcatt ctctcccgca cctacagcag ctaccggacg
     1801 aagacgcctg caccggtggg cgttttcggc cccggctgga aagcgccttc tgatatccgc
     1861 ttacagctac gtgatgacgg actgatactc aacgacaacg gcgggcggag cattcacttt
     1921 gagccgctgc tgccggggga ggcggtgtac agccgcagtg agtcaatgtg gctggtgcgc
     1981 ggtggtaagg cagcacagcc ggacggccat acgctggcgc ggctgtgggg ggcgctgcgc
     2041 cggatatccg gttaagcccg catctttacc tggcgaccaa cagcgcacag gggccgtggt
     2101 ggatactggg gggggctgag cgggtgccgg gtgctgagga cgtactgcca gcgccgctgc
     2161 cgccgtaccg ggtgcttacc gggatggcgg acgctgacgt accggcgtga ggccgccggt
     2221 gacctggccg gggaaatcac cgacgtgacg gacggtgccg ggcgggagtt ccgtctggtg
     2281 ctgaccacgc aggcgcagcg tgcggaagag gcccgcacct cttcgctatc ttcttctgac
     2341 agttcccgcc ctctctcagc ctcagcgttc cccgacacac tgcctggtac cgaatacggc
     2401 cccgacaggg gtatccgcct ttcggcggtg tggttgatgc acgacccggc atacccggag
     2461 agcctgcccg ctgcgccact ggtgcggtac acgtatacgg aagccggtga actgctggcg
     2521 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     2581 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacg
     2641 gggcgggtgg tggagcaact gaacccggca gggttaagct accgctatct ttatgagcag
     2701 gaccgcatca ccgtcaccga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2761 gccgggctga aacgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcgcagcggg
     2821 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
     2881 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2941 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtacccgga cgggctggag
     3001 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
     3061 acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg
     3121 acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac
     3181 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac
     3241 cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg
     3301 aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc
     3361 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaagacggtc
     3421 agcaccacgc agggcgggct gacgcgcagt atggagtacg atgctgccgg acgtgtcatc
     3481 agtctgacta acgagaacgg cagtcacagc gacttcagct acgatgcgct ggaccggctg
     3541 gtacagcagg gcggctttga cgggcggacg caacgttatc attatgacct gaccggaaaa
     3601 ctcacgcaga gtgaggatga ggggcttgtc accctctggc actacgatgc gtcggaccgc
     3661 atcacgcacc ggacggtgaa cggcgacccg gcagagcagt ggcagtatga cggccacggc
     3721 tggctgagag aaatcagcca cctgagcgaa ggccaccgtg tcgctgtcca ctacggctac
     3781 gacgataagg gccgcctgac gggcgaacgg cagacggtgg agaacccgga gacgggggaa
     3841 ctgctgtggc agcatgagac gaaacacgcc tacaacgaac aggggctggc aaaccgcgtc
     3901 acgccggaca gcctgccgcc ggtggagtgg ctgacctacg gcagcggtta cctggcgggc
     3961 atgaagctgg gcgggacgcc gctggtcgag tatacgcggg acaggctgca ccgtgagacg
     4021 gtgcgcaccc tcgcaccgga cctggacatc cgcatcccgt atgccaccga cccggcggga
     4081 aaccgcctgc cggacccgga gctacacccg gacagcacgc tcacggcgtg gccggataac
     4141 cgcatcgcag aggatgcgca ctatgtttac cactatgatg aatacggcag actgacggag
     4201 aagacggacc gcatcccgac gggtgtgata cggacggacg acgagcggac ccaccactac
     4261 gacagccagc accgcctggt gttccacacg cggatacagc atggcgagcc actggtcgag
     4321 agccgctacc tctacgaccc gctgggacgg cgaatggcga aacgggtatg gcggcgggag
     4381 cgtgacctga cggggtggat gtcgctgtcg cgtaaaccgg aggagacatg gtacggctgg
     4441 gacggcgaca ggctgacgac ggtacagacc gacaccacac gtatccagac ggtatacgag
     4501 ccgggaagct tcacgccgct catccgggtc gagacagaga acggcgagcg ggagaaagcg
     4561 cagcgccgca gcctggcgga gaagctgcag caggaaggga gcgaggacgg gcacggcgtg
     4621 gtatttccgg ctgaactggt gcggctgctg gacagactgg aggaagaaat ccgggcagac
     4681 cgcgtgagca gtgaaagccg ggtgtggctt gcgcagtgcg ggctgacggt ggagcaactg
     4741 gccagacagg tggagccgga atacacacca gtgcgaaacg ttcatcttta ccactgcgac
     4801 catcggggcc agccgctggc gctcatcagc gaagacggcg atacggtgtg gagcggggcg
     4861 tatgatgagt ggggcaacca gctaaatgag gagaacccgc atcatgtgta tcagccatac
     4921 cgtctgccgg ggcagcagta tgatgaggag tcggggctgt actacaatcg tcaccggtac
     4981 tatgatccgt tgcagagacg gtacatcacc caggacccca ttgggctgaa aggtgggtgg
     5041 aatctgtatc agtatccgct gaatccggtg agcaggatag atccgttggg gttattctca
     5101 tgtgagagtt gtagtcattt tatagatgat ataaataata aactggataa ccttccgttt
     5161 aacccgacaa aaatgtttac agctagttta aatactgcta attcagcaag gctgtatgca
     5221 agtggcatga caaaattcgg tataagtgtt gctgccgatg gaacgatcat tgctgcccca
     5281 gtcggtgcag cttctatggc tttaggggcg tggaatataa aatctgcgac agctgcatat
     5341 aacagagcaa atttacaatt atcagaatca cttcaggaaa atggtagaga taggtgttgg
     5401 agaaatttat tgggtatttt accttatggt gagcagtttg atgaccctgg tgaaccatca
     5461 atattagacg tttatggtga aaagattgag aatatcaaag attctccaat gaagtttttt
     5521 gaagagcttg ggacgctggg gttgtaatgt gtatttttaa tgatattttt atgtttgtgt
     5581 ttattgttag tagtatactt gtaaccgtgc attttgtgta ttctttattt tttaaaagtg
     5641 gaaaggtaaa agataaggta aacaaatgga ttaaagtaat gtgtgattta ttttggggga
     5701 taggatagtg gtgtgatatg gataaatata aattacagtg gctggcatat ttcgacctga
     5761 cgccggagta gataaaaaaa cagacaggat tggagtatag caagcgtctg acaatctctc
     5821 gggacagtag tatgatgaag aggcggggtt gtactataac cgtaaccggt actacgatcc
     5881 gctaaagggc aagtatatga c
//
LOCUS       DAZPEP010000210         5865 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZPEP010000210 DAZPEP010000000
VERSION     DAZPEP010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.212"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..560)
                     /locus_tag="ACHUT9_003962"
     CDS             complement(<1..560)
                     /locus_tag="ACHUT9_003962"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HFR1350326.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIR"
     gene            complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHUT9_003963"
     CDS             complement(704..1462)
                     /gene="aroD"
                     /locus_tag="ACHUT9_003963"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFR1350327.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHUT9_003964"
     CDS             complement(1493..2359)
                     /gene="ydiB"
                     /locus_tag="ACHUT9_003964"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFR1350328.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(2371..3636)
                     /locus_tag="ACHUT9_003965"
     CDS             complement(2371..3636)
                     /locus_tag="ACHUT9_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1350329.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKAYGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3863..5077)
                     /locus_tag="ACHUT9_003966"
     CDS             complement(3863..5077)
                     /locus_tag="ACHUT9_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1350330.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(5177..5533)
                     /locus_tag="ACHUT9_003967"
     CDS             complement(5177..5533)
                     /locus_tag="ACHUT9_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFR1350331.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(5669..5776)
                     /gene="rprA"
                     /locus_tag="ACHUT9_003968"
     ncRNA           complement(5669..5776)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHUT9_003968"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
BASE COUNT         1690 a         1339 c         1329 g         1507 t
ORIGIN      
        1 cgaatgaagg cgatatctgg cgcaatcgct ttgtaataga gatattcttt gttatcaaac
       61 tcgaccagtt taatcaggtc ttctttcgtg acttcattca gtttgccgcc ttgctggcgt
      121 ggatcgacaa atgtcccgat gccaatatca ctaataatac caggctggtg ggctgcggcg
      181 gcgcgtaagg tttgtgtaag tacaccttgt ggatagttat aagcaataat tttattttgt
      241 tctgcgagtt cagaaatacg cggcgattgt ccccagtgac cacataatgc ccatttcacc
      301 agaccttctt gcgccagagg actaatacca cggtcggcgc gatcgccaag ccctgtcgga
      361 ctaataatcg ataaattacg tggcgtttga gtctgtttat atttatcagc aagagctgta
      421 attaacgtgg tggcttccag aataccgccg ccagcgccta acacacaaag tgttgcttcg
      481 tcgggaatat aattcaccgc ttcctgtgcc gacaggaccg gcactcgtcc attaatacga
      541 ggtggtttta caggtttcat tgttaattcc tgattcagat gtgattttcc ctacgccaga
      601 tggagaatta gcgcacagag actcacgaaa tatttttatt aaatacggtt aaatatattt
      661 tttaagttcg gcggggaggg tgttcccgcc gaaatattat tgcttatgcc tggtgtaaaa
      721 tagttaatac cgtgcgtaaa tcatttaccg agatttgccc tggtgcagag gcttttttta
      781 ccgcaccaaa agttgccgcc gagccaaata cttcaccagc cagacgagaa attacgccag
      841 tttttgccat cgacatcgtg atgattggac gatcggcata ctgctcctgc atctccaggg
      901 tcgcggtaag caacgtcagc acatcgctgg tactttgcgg catcagcgca atcttaggaa
      961 tatcggcgtc gaaggactgc attttgcgca gacgggcaat gatttcttcg gcttccggcg
     1021 ttttatggaa gtcatggttg gacatgacaa ctttcacatc atgcgcgtgg gcgtaggcga
     1081 cggtttcttt aacctgatca tcgccggtaa ataactccag atcgatcata tcaaccaggc
     1141 cgctgtcgat ggctgcacga ttgagagcaa tataagcctc ggtggaaatc gcctgctcgc
     1201 cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt ttctggcatg gtttcacgga
     1261 gaatttttgc cgccgccatg acagactcca cattggagag gtcggcaaag tggtccacac
     1321 gccattccag aatatcaaag tccgcttcac gataggcgag agcttcggat ttcacgcggg
     1381 cgatatcttt cgccatcagc gagacgatga ttttaggtgc gcccgcacca atgacgagat
     1441 cttttacagt tacggttttc attttttacc ctttctgcac gcggtcagcc tgtcaggcac
     1501 cgaaccccat gacctgttta acatattcca gagggaaatc tttgccagtc cataacgtga
     1561 actgttcagc cccttgccac aacaacatgc cgtatccatc aatcgttttg caacctgctt
     1621 gttgtgcctg ctgcaataac ttcgtcatat gcgggttata cacgcattca gtgaccagca
     1681 gtcccggatg taacagactg atatcattaa ccaatgattc attctcaagg gggttcatac
     1741 ctacttttgt gccattggtt aaaatgtcgg cggaagccag gacttcagca aaggcttgct
     1801 gatcggcgag atcggtaacc gtgacgacac aatcggtgtt ttcgttaacc cgctgcgcga
     1861 aggcgagggc tttatcgaag aactcatccc gacggttaaa gagtttaatt tcttttaaac
     1921 cttcaattgc cccctgcgcg ccaatcgctg ttgaggcacc tccggcccct aacagcacca
     1981 tcgttttgcc tttgatatca aaaccgctct ctttaatggc gcgaatatgg cccgtgccgt
     2041 cggtgttata gccacgcaga tagccatcat cattaacgat ggtgttgatg gcacccacca
     2101 gtttggctgc tggcgttaat tcatcaacat attcacacgc cagttgtttg ttcggcatcg
     2161 acacaccagt tccgcgcatt ttgagggctt ttaatccttc aattgctccg ggaaagctat
     2221 cgttatccac ttcgaaggcc atataggtaa atggcaatcc cgctttttct aaggctttat
     2281 tctgcatttc aggcgataaa ctgtggcgga taggataagc catcaaccca atcaattcgt
     2341 attttgcggt aacatccata attaactcct ttaacctcta tgcttaattg tgtttaaccg
     2401 ggtgctaaat ttgcgttcgc caaaccgcac atcattctca ggaataataa aaacgcggta
     2461 atagcggcta aaaacaataa ttgcggtaat cagggccagc agcgcgaaag taaaatcgag
     2521 aacaatgata tattgcaggc cgatgttcga cagataaccg gtaatcagcg gaataacaaa
     2581 gttagccagt ccacccatca tcatataaat actggtgact ttggctttgc ttttggggaa
     2641 aaactctgac atgaccgaaa caccgagctg taaaatgccg ccagctgctg agaaaccgat
     2701 aacaaaggcg ccggcattac acaccagtgg agaagggtac aggtagataa tggcagctgt
     2761 tattgttgcc agcgcagagt taaatacatt agcccagatg ggccggacca tttttttcag
     2821 tagtgcggca aaaataaaga cacagaccaa cgagcccata ctgtaataag agatggtttt
     2881 taatgcctca gcttctgaca taccagcaaa agccatcgca tatttgggca tccacaccac
     2941 aatcacataa aaggtcgaga atgcggctac accgaacagt accgatgaaa caccttccag
     3001 ccagactaac ggtttgctgt tcatttgcgg taattcatta gctacgctgg cgtccaccaa
     3061 ctggctgggg aatttgcttt tcaacagcat cagcgtgatc agtacaaaca gaatacccgg
     3121 aataatcagc ccatagccgt accagatatt attgagcaac atatagctca ccagcattgg
     3181 gtagaacatt tgcccaaatg acaccatcgc tttaaccagt atgaccgccg aaccataggc
     3241 tttcggaaag cattccatga gcgcggggta gccacccgta tccagcgctg agttagcgat
     3301 acctacgcac actgccagac cgtaggcgag agttaaattc gggcaagcgg gaataccaaa
     3361 gaagaatagc agatacatta ttactgccat taatatcacc gcccgacgac caaacttatc
     3421 ggagatcaca ccgaagaata aaatactgat caatcgcccc aaaccgatac cggaaattaa
     3481 ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc agagatgtca tattttgggc
     3541 aagcgtaata acactaatgc cgtgcaggaa atagctgaag taaatacaaa gaacagccag
     3601 gataaatggc gtgctgaaag ccttattttg agacattttc acagctcctt gctgaaagat
     3661 aatttcgtta ccgtacgtat tgttctccga cggtttacat aattactata ctgaagatga
     3721 ttttccctgg gtacatggca taaagtttat gtttccagga atattgcatg tcatagtttg
     3781 tttttgatcg cgattttcct tagttagcgc agaaagagtt atttatccgt aagatcttgc
     3841 atgacctgaa aagaatattg cattacccac ccggagcgac attttccttt agcgagtgat
     3901 gccgcgtttt tttgcggctg cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag
     3961 cgatggcggt atcgaaccac ataatatcgg caatacttct ttgggacaga tgagccgtaa
     4021 tcaacggaat agtaaaggtc gcaatactgc ccgcactgta atagatccct gtagctttac
     4081 ctttagcgta agggaaacgt tcagccatta acgtcaggcc aatttgcaca acacctccag
     4141 cagaggtaaa accaatgaca aaagcaaata ttatcaccac ataaaatgtg ggatgcaggc
     4201 agacggtaaa cagagcaata aatgagataa aggtgtacag catcagtaat gttgttgggc
     4261 gaacggtatt acgaatgagt ggagcggtaa taaatacaca aagcagcgaa cccacggtgt
     4321 agatactgag tagtttgatc gacatagtgt atgacatgcc tgcaacaaat tgtccgtact
     4381 gtgccagcca ctggctaacc agataaaacg ttgccattga gatatagcca tataaggtat
     4441 aactggctaa atcgataatt gaacagcgat gttccgtaga gctggtggtt ttctttatga
     4501 caggtaagcg acgacccgga tggggtggga acgtacaacg gtataaaaac agagcgttaa
     4561 taaacataat gcctgcagca atcataaagg accaaccgaa ccacagttca gcccacacta
     4621 acaggctaat gattagcggt aataaaaatt gtccgctgga aacaaatgct ttaattaaaa
     4681 tattggctgt cccaggtgag cgtggaaaag cttccatcaa actgggataa gtgcctgcat
     4741 cgagaaaact gtttgccatt cccgccagaa agccaaaaac ataagcgata atgatgttat
     4801 tggtctgcag gatgccaaaa aagaaggcca tatagcagca catcccgagc atgataaaag
     4861 ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag caagacactt aatcgaccaa
     4921 tgcccagcga tgagataact atcgagacac ccgcggcatt agtctgccaa agtgtctcca
     4981 gcgaggccat attcaggctc atcaaaatga cgcccatacc atgcaccagg taattaaaat
     5041 acaacccaag tgcggtaggg taatagggat ttttcatagg ttctacttag caaaggtgct
     5101 ttaaaaagtg tagcgatgtt gtgtcagtat ccattcatta ttgttgtaac aacgacaatt
     5161 cctttctgtg gaaaaattaa caaagccgct ccagatcgtg tgcatataat tcagcggcta
     5221 ccgattgata gatcttccag tcaataaaat tgccatcagg gtagacttgt tgaaacgcgg
     5281 ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg gttatttatt ttctctatga
     5341 tggcatgaag atgttttttt cgttgctgac gcatagccaa cagttgctcg actacacaat
     5401 caggaatggc gcatttgcca ttttcccact gacgccagct ttcactgtca ccggtctggg
     5461 caatccaggt cgcgcattca tcaatagtca tggcaaaaat atggcggagt gcctggagtt
     5521 cataagcgtt catcgtgtaa tggctgcatg aaataaacaa taaaatataa ataacattat
     5581 cagcctgctg acggcttgaa gagagtcaca gtatcttgtg caacattatt ggaaagagtg
     5641 aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc ccatcgtggg agatgggcaa
     5701 agactacaca cagcaattcg ttgtttcact caggggattt ccatgcttat aaatcaatat
     5761 gttgatttat aaccgtgagc taatagtagg catacggact ttttgcgtcg atcagattcg
     5821 tctcattagt ttaaaaactg taaagaattt gcgacaggta ctacc
//
LOCUS       DAZPEP010000211         5862 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZPEP010000211 DAZPEP010000000
VERSION     DAZPEP010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.213"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..353
                     /gene="traA"
                     /locus_tag="ACHUT9_003969"
     CDS             <1..353
                     /gene="traA"
                     /locus_tag="ACHUT9_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFR1350332.1"
                     /translation="LSVQGTSAPVKKKSFFSKFTRLNMLRLVRAVIPVAVLMMLFPEL
                     AMAAGKGTDLMAKGQETVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVGFAI
                     LSVFIAVGMSVAGF"
     gene            368..679
                     /gene="traL"
                     /locus_tag="ACHUT9_003970"
     CDS             368..679
                     /gene="traL"
                     /locus_tag="ACHUT9_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFR1350333.1"
                     /translation="MLGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICVGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            701..883
                     /locus_tag="ACHUT9_003971"
                     /pseudo
     CDS             701..883
                     /locus_tag="ACHUT9_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraE/TraK family type IV conjugative transfer
                     system protein"
     gene            885..2135
                     /locus_tag="ACHUT9_003972"
                     /pseudo
     CDS             885..2135
                     /locus_tag="ACHUT9_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
     gene            2132..2518
                     /gene="trbI"
                     /locus_tag="ACHUT9_003973"
     CDS             2132..2518
                     /gene="trbI"
                     /locus_tag="ACHUT9_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFR1350334.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PMTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            2515..2826
                     /locus_tag="ACHUT9_003974"
                     /pseudo
     CDS             2515..2826
                     /locus_tag="ACHUT9_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
     gene            2821..3819
                     /locus_tag="ACHUT9_003975"
                     /pseudo
     CDS             2821..3819
                     /locus_tag="ACHUT9_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI
                     domain-containing protein"
     gene            3839..4586
                     /gene="traX"
                     /locus_tag="ACHUT9_003976"
                     /pseudo
     CDS             3839..4586
                     /gene="traX"
                     /locus_tag="ACHUT9_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            4641..5201
                     /locus_tag="ACHUT9_003977"
     CDS             4641..5201
                     /locus_tag="ACHUT9_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFR1350335.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTLRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            5331..5543
                     /locus_tag="ACHUT9_003978"
     CDS             5331..5543
                     /locus_tag="ACHUT9_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFR1350336.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1379 a         1420 c         1792 g         1271 t
ORIGIN      
        1 cgttaagtgt tcagggtact tctgcgcccg tcaaaaagaa atcgtttttt tctaaattca
       61 ctcgtctgaa tatgcttcgc ctggttcgtg cagtgatccc ggttgctgtt ctgatgatgt
      121 tgttccctga gttagctatg gctgctggta aaggcacgga cctgatggct aaaggtcagg
      181 aaacggttaa ggcgaccttc ggtaaggact ccagtatcgt taaatgggtt gttctggctg
      241 aagttctggt cggtgcagtc atgtacatga tgaccaaaaa cgtcaagttc ctggtcggct
      301 ttgccatact ctctgtattt attgcagtag gtatgtctgt tgccggattc tgacaggaaa
      361 taaaacgatg ttgggagacg agaataaact taagaaatat cgtttcccgg aaacactgac
      421 caaccagagc cgctggtttg gcctgccact ggatgaactg atccccgcag caatctgtgt
      481 tggctggggt atcacaacat cgaaatatct gttcggtatt ggtgcagcgg ttctggttta
      541 tttcgggatt aaaaaactga aaaaagggcg gggcagttcc tggttacgtg acctgattta
      601 ctggtatatg ccaacagccc tgctgcgcgg tatttttcat aatgttcccg attcgtgttt
      661 ccggcagtgg attaaataga actgatacca ggattgttat atggaacacg gtgcccgttt
      721 aagtaccagt cgtgtaatgg ccatcgcctt tatatttatg tcagtgctta ttgttctcag
      781 cctctctgtt aacctcattc agggggtgaa taactaccgt cttcagaatg agcaacgcac
      841 tgccgtgacc ccaatgggat ttaatgtgcc ctttgccgtg tcacacctac cgtaaccagc
      901 tggcgtttat cgatattttc ttccggggaa tgaataacac caactacaac atggcggtct
      961 gtggcacctc cggggccggt aaaaccgggc tgatacagcc acttatccgc agcgtgctgg
     1021 actccggggg ctttgccgtg gtgttcgaca tgggggatgg ctacaagtcc ctgtgtgaga
     1081 acatgggggg cgtatatctg gacggtgaaa ccctgcgttt taacccgttt gcgaacatca
     1141 ccgatatcga ccagtcagcg gagcgtgtcc gtgaccagtt gtcagtgatg gccagcccca
     1201 acggtaacct ggatgaagtg catgaaggtc tgctgctgca ggcggtcagg gcctcctggc
     1261 tggccaaaga gaacagggca cgtattgatg acgtggtgga tttcctgaaa aacgccagtg
     1321 acagcgagca gtatgccggg tcaccgacta tccgcagccg tctggacgaa atgattgtgc
     1381 tgcttgacca gtacactgcc aacggcactt acggccagta ttttaactct gatgagccgt
     1441 ccctgcggga tgacgcaaaa atggtggtgc tggagctggg cggactggaa gaccgtccgt
     1501 cactgctggt tgcggtgatg ttctccctga ttatctacat cgagaacagg atgtaccgca
     1561 cgccgcgtaa cctcaagaaa ctgaacgtca ttgatgaagg ctggcgtctg ctggacttca
     1621 aaaatcacaa ggtcggtgag tttattgaga aaggttaccg taccgcccgt cgtcataccg
     1681 gtgcttatat caccatcaca cagaacatcg tcgactttga ctctgacaag gcctccagtg
     1741 cggcccgtgc ggcatggggt aactcctcct acaaaattat cctcaaacag agtgcgaagg
     1801 agttcgcaaa atacaaccag ctgtatccgg accagttcca gccactgcag cgcgacatga
     1861 ttggtaagtt tggtgcggcc aaagaccagt ggttcagttc cttcctgctg caggtggaaa
     1921 accattcctc ctggcaccgc ctgtttgtgg acccgttaag ccgcgccatg tacagctctg
     1981 acggcccgga ttttgagttt gtgcagcaga aacgtaaaga ggggctgagt attcatgagg
     2041 cggtgtggca gctggcgtgg aagaagtcag ggccggagat ggcttcgctg gaagcctggc
     2101 tggaagaaca tgagaaatac aggagtgttg catgaccaca acgcaaaaaa cgactgatgt
     2161 tacagccccc cgacgcagcc actggtggtg gacggtgccg ggatgcctgg caatggtgtt
     2221 actgaacgca gccgtcagtt acggcattgt cagactgaac gcaccgatga ctgccgcctt
     2281 caacatgaaa cagactgtgg atgcattttt tgacagcgcc agtcagaaac aactgtcaga
     2341 agcacagtca aaagcccttt cagcgcgttt taacacggca ctggaagcca gcctgcaggc
     2401 gtggcagcag aaacaccacg cggtcattct ggtgtcgcct gccgtggtac agggggctcc
     2461 ggatatcacc cgggaaatcc agcaggatat tgcccggcga atgagagcgg aaccatgaga
     2521 tgccgggggc tgattgccct gctgatatgg gggcagagtg tggccgccgc cgatcttggc
     2581 acctggggtg atctgtggcc ggtaaaggag ccggacatgc tgacggtgat catgcagcgc
     2641 ctgacggcac tggagcagtc cggtgagatg ggccggaaaa tggatgcctt taaggagcgg
     2701 gtgatccgca acagtctgcg gccccctgcc gtgcccggta tcggacgcac ggagaaatac
     2761 agcagccggt tgtttgaccc gtccgtcaga ctggctgcgg atatccggga taacgaaggg
     2821 cgggtggccg gctttgagtc agcctacgtg gccctgtcgc gtatgaagca gcatgtgcag
     2881 gtgtacaccg ataaccgtca gggctggacg gatgccatca acaatgccgt acagaaagga
     2941 actgcccacg atgtgtttga gccgaaaccg gaccgggagg tcatgaatgc agagcggctg
     3001 ttcagtacgg cgcgggagct gcgggacgtg gcggcaggcc gtgccgttct ccgtcaggcg
     3061 gggctggccg ggggagacag tcctgcacgg tttattgctc cgggacgtaa atatccgcaa
     3121 ccgtatgtgg cactgccggc gtttgaccgt aacggcaagt ccgccggtat ctggctgaac
     3181 ccgctgacca cggatgacgg aaacgggctg cggggattca gcggtgaagg tcgggtaaaa
     3241 ggcagcgggg atgcgcagtt cgtggccctg cagggcagcc gtaacggaga aagcctgctg
     3301 gctgataata tgcaggatgg cgtccggatt gcccgtgata atcctgacag tggtgtggtg
     3361 gtaagcattg ccggtgaagg ccgtccgtgg aatcccggtg ccatcaccga tggccgcgtg
     3421 tggggggata tcccggacaa cagcgtccag ccgggagccg gaaatggcga accggtcacg
     3481 gcagaggtac tggcacagcg gcaggctgaa gaggccatcc gccgtgaaac ggaacgtcgc
     3541 gcagatgaaa ttgtccgtaa aatggcagag aacaaacctg acctgccgga cggcaaaaca
     3601 gagctggctg tcagggaaat tgccgggcag gagcgtgacc gggctgccat aactgaacgg
     3661 gaagccgcgc tgccggagag tgtgctgcgt gaatcacaac gggagcggga agcggtccgg
     3721 gaggttgccc gggaaaatct gctgcaggag cgtctgcagc agatggagcg ggatatggtt
     3781 cgtgacctgc agaaagagaa aaccccgggc ggagactgat acaggaagac aaacactgat
     3841 gacaacggat aacacgaaca cgacacgtaa cgattcactg gctgcacgga ccgatacctg
     3901 gttgcagtca tttctggtct ggtcacccgg acagcgggat atcatcaaaa cggtggcact
     3961 ggtgctgatg gtactggacc acatcaatct gatattccag ctgaagcagg aatggatgtt
     4021 tctggccggg cgaggagcct ttccgctttt tgccctggtg tgggggctga atctgtcccg
     4081 tcatgcgcat atccggcaac cggccattaa ccggctgtgg ggatggggaa ttattgccca
     4141 gttcgcatat tatctggcag gctttccctg gtatgagggg aatatcctgt ttgcctttgc
     4201 agtggcggca caggtgctga cgtggtgtga aacgcgcagt gggtggcgta cagcagcggc
     4261 cattcttctg atggccctgt tgggggcctt tgtccggcac cagttacggc attgccgggc
     4321 tgctgatgct ggcagtcagc caccgactgt accgggcgga agacagagcg gaacgtctgg
     4381 cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa ccttgccacc agtgatgcag
     4441 cggcggtggc cggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg tgtgcaggaa
     4501 aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc tgtcatctgg
     4561 ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa caggatgatt
     4621 cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca ctgaagcgga
     4681 aaacggaagg ggagacgcct gtccgcagcc ggaaaaccat catcaatgtc accacgccac
     4741 caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa gcagagctgg
     4801 cggcaaaaaa agcgcaggcc agacaggcac tgtccattta tctgaacctg cccacgctgg
     4861 atgaggccgt gaataccctg aaaccctggt ggccgggatt atttgacggt gacaccctcc
     4921 ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag cggaatatcc
     4981 cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca gaaagctatc
     5041 tgtgtgccat gaaagccggt gcctgccggt atgacacgga agggtatgtg acggagcata
     5101 tttctcagga ggaggaagcg tatgcggcag cgcgtctgga taaaatccgc cgccagaacc
     5161 ggataaaggc agaacttcag gccgtgcttg atgagaaata aaaaaaagcc tccatcagag
     5221 aggaggcagg gaaataaggc ttaaaaggaa ataacttccg cttccgaaag gaagccagtc
     5281 cactgaatta tttatcacaa taatactgac aactaaaggg ggacaagaaa atgaatggat
     5341 tcagaaacag ttcacggaac ggccaggtct ggcgttacca gcgtgccggc gggcgggccg
     5401 ttattctgga agtcagtgga cgctggatgg aagcggcaga agcatggcga cgggctgcct
     5461 gtgttgcgcc caggacagac tggcagcagt ttgcccgaaa aagggctgaa cactgccacc
     5521 ggcgctgccg gggaagggtg taacgaaaaa agcagccttc attactccag gagtcagaag
     5581 gctgaaagga gtttatgaga tttgcttttt ttaatatccg gtcgggcagt atgaccggac
     5641 agttctgagt agcacagagt tttttccgta ttaagtcgtg attttatatt ttgtgattaa
     5701 tttcacaaaa taaggtgtta ttcagtgtgt gctgcaatat tcaggatgcc tgaaatacag
     5761 atgctattta tctgatatgg agaataacat gaggaaatag taaggcagta tgactttccg
     5821 tgagacagat gctggccaac gtgggatatt taacgcgggt ta
//
LOCUS       DAZPEP010000212         5839 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZPEP010000212 DAZPEP010000000
VERSION     DAZPEP010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.214"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(155..949)
                     /locus_tag="ACHUT9_003979"
     CDS             complement(155..949)
                     /locus_tag="ACHUT9_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1350337.1"
                     /translation="MSDAITTAAIKKLYGLSAGQCNICRTSLFKEQVHIGQMAHVIAK
                     SPKGPRGDDLLANDNSYENLILLCANDHILVDRDPFTYTVEKLHQIKSEHEAYIQGAT
                     DNTLFSDKKRQSDVDFLNAYFHFTPFARVRSLVETLPNSFHINLLIFGDQFDSILRDF
                     PASYPLNDKLLHGHFQSFIDSYKNIDFIISGHLPISDNRNIQVFGGANHNLLCHFNYD
                     ELPSDKALIIEQDLISHKISLLNAYEGLINYLRAYYPEVEIYSPIL"
     gene            complement(1010..1303)
                     /locus_tag="ACHUT9_003980"
     CDS             complement(1010..1303)
                     /locus_tag="ACHUT9_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350338.1"
                     /translation="MISILIEFLKSGLDFFIKKKTIEQEVKKTNVEGQIETNKEEIEK
                     VTFHWRNALGFVLTLIILYNWIIVPVLDAFGIIVIQVPLGQLLQVLLIMVGGS"
     gene            complement(1300..1611)
                     /locus_tag="ACHUT9_003981"
     CDS             complement(1300..1611)
                     /locus_tag="ACHUT9_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350339.1"
                     /translation="MNFEQHYGDLISKIKLNGMTPDGNSMPINKCFLDKKFQKVVKKS
                     NYQIDSYMNVIYEKKKDFKVGEVLEWELQGEKMPVFLIESKKLFVKGKHFWVYAVGII
                     E"
     gene            complement(1687..2187)
                     /locus_tag="ACHUT9_003982"
     CDS             complement(1687..2187)
                     /locus_tag="ACHUT9_003982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350340.1"
                     /translation="MASGINGIGSIRNYIQTQGVRLNNQFQNEIIKRSKLLSQRMQGD
                     LNNSVDRGAVGFTQRSILFFYRKVGRNSVTSTIMVKDIQAKYLYQVLVQPKAIDKFIP
                     TSSTRLTKQGNISGLKKNLLSERYKVVKGKNGKERLIDTAKKDTKKKTKRVIGLREKK
                     QRRLIY"
     gene            complement(2197..2631)
                     /locus_tag="ACHUT9_003983"
     CDS             complement(2197..2631)
                     /locus_tag="ACHUT9_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350341.1"
                     /translation="MMSEWKYSDDWSEEEMLSGEYAGFVYIFQFEDGSNYVGAKQIYQ
                     RVKDARKLKPTSKENGWREYLSSSNVVKNKIENGEQYSKTILWCFATMREVMIVEAIL
                     ILHQMLKPECLNLAMMSKIRSPNAKDKKRLLGIVQELLEYLN"
     gene            2668..3117
                     /locus_tag="ACHUT9_003984"
     CDS             2668..3117
                     /locus_tag="ACHUT9_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFR1350342.1"
                     /translation="MSDLKTRLKEYEGTKEYQANLKYFRNGKFYPYADSLGCSTIVYG
                     HLIQAGEDFSTGITESEADKLLSRDLAKTIMQVQTLGLNLPDDWNDFIIIMTFQLGIG
                     GVKKFRKMLAALKAHDYLEAIKQAKCSLWYRQTPNRVNSMIAVLKNK"
     gene            complement(3156..3428)
                     /locus_tag="ACHUT9_003985"
     CDS             complement(3156..3428)
                     /locus_tag="ACHUT9_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFR1350343.1"
                     /translation="MDIGAILALVISGCVFLYTIFRDNTKDTNDLLTRVATLETTIAV
                     LESSISRIEDDQDKMRDTLSKLETQIHELDVKIERIIAILQQKQRP"
     gene            complement(3497..4153)
                     /locus_tag="ACHUT9_003986"
     CDS             complement(3497..4153)
                     /locus_tag="ACHUT9_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350344.1"
                     /translation="MAGFEVYNSAGALTIDSTNKSIMTGAVKAMGNLTDTGYYTGFTC
                     AFGNGGSLGFVMPSVVANRNTTQYWFQIQKDGAWCFPGAYMFQPGMGRFMTSSHTATP
                     TSGFLDVFSEEGTLIWSAASAATMPRIRGFLTAPAATDLSTAITVTSPVADPWFCWSQ
                     CPGNISDDGTVIGYSGLVIRRNSSTSFSLQYVSKNQKTYRQAMGNNGIQIALATFTGY
                     "
     gene            complement(4155..4451)
                     /locus_tag="ACHUT9_003987"
     CDS             complement(4155..4451)
                     /locus_tag="ACHUT9_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016042153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350345.1"
                     /translation="MAQGLQCWNAAGVLVVDLTDYNIRFMGTYTVKATSAQTYTVSVP
                     NMKTTGWFVHYAPASDTFNEWSAFCNNGSFTAVYLPTYAPLAGNYTFNVYKWTA"
     gene            complement(4609..>5839)
                     /locus_tag="ACHUT9_003988"
     CDS             complement(4609..>5839)
                     /locus_tag="ACHUT9_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001175757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350346.1"
                     /translation="AQGFNDGTYSEEVKITVENKQCGLVEDVSFTGGFGNLFASWTKS
                     TERDYAGAIISIVNGTNTRIFTSYAPEFDSIPNITDGEYKVKMGFFDVFGTDDIQYCP
                     EQTISINSKYQFTEDDAEAINGILDLEDRLTGTINDAVKIANKNTSTVISASEARTND
                     KITASEKTLSTQITGVNSSLSQKLSTVESTANGNKASIQTLSQTVTDNNKAQTTAVSQ
                     LRSDVNGQIATVNQSMSTKADKSTVDAQYSLSVQANGTVAGMRLVASSGTSNNSAIYF
                     AANKFIVSGTDTATVGGTAPFAIVNGTTYLKTAMIQAASIGTGYIADAAITNLKLASG
                     SVNTLNVVDGCITTAKISNTIQSTNYVANKTGWQINKAGTILINGTGGTGRMVISNNI
                     IQIYDNNNTLRVRMGLW"
BASE COUNT         1749 a         1205 c          989 g         1896 t
ORIGIN      
        1 tcggctttat taaattacaa aaaaataaag aaaccagatg ttagggaaat aataaaacaa
       61 agccattcgg tttttagaga aaacgaagaa accattacta ttacaacaac cataaaaaaa
      121 gccccataag gggcttttat atatttcata acactcatag aataggagag taaatttcca
      181 cctcagggta ataagctcta aggtaattta taagaccttc ataagcatta agaagagata
      241 ttttatgtga aatcaaatct tgttctatta tcagtgcttt atcggatggt agctcatcat
      301 aattgaaatg acaaagaaga ttatgattcg caccaccaaa cacctgaatg tttctattat
      361 cggaaatcgg caaatgtcca gatatgataa aatcaatatt tttatacgaa tcaataaaac
      421 tttggaaatg accgtggagt aatttatcat ttaaaggata gctcgctgga aaatccctta
      481 aaatactatc aaactgatct ccaaaaataa gaagattgat gtgaaaagaa ttaggtaaag
      541 tttcaactaa tgaacgtacc ctcgcaaacg gtgtaaagtg gaagtaagcg tttaaaaagt
      601 ccacatctga ctgtcttttt ttatctgaaa ataatgtatt atcggttgca ccctgtatat
      661 aagcctcatg ttcacttttg atttgatgca atttttctac tgtataagtg aatggatctc
      721 tatcaacaag aatatgatca tttgcacaaa gcaggataag attttcataa gaattgtcat
      781 ttgctaacag gtcatcgccc cttggtcctt ttgggctttt ggctattaca tgagccattt
      841 gtccgatgtg aacctgctct ttaaaaagag aagttcggca aatattacat tgcccagcgg
      901 atagtccata caattttttt attgctgctg tagtaattgc atcactcata aatcaaatcc
      961 ttagattgct attttattaa ctcaaaacac atattaaagg aaaaatcttt cagcttccac
     1021 caaccataat caatagcact tgcaaaagtt gtcctaatgg aacttggatc acaataattc
     1081 caaaagcatc aagtactggt actatgatcc agttatagag aatgataagg gttagtacaa
     1141 aaccgagggc attacgccag tggaatgtta ccttttcaat ctcttcttta ttcgtttcaa
     1201 tctgcccttc tacattcgtt ttctttactt cctgttcaat agttttcttc ttaatgaaaa
     1261 aatccaatcc tgacttcaga aactcaatca atatactaat cattcaataa tccccacggc
     1321 atatacccaa aaatgtttac ccttcacaaa gagcttttta ctctcaatta gaaatacagg
     1381 catcttttca ccctgtaatt cccattcaag tacttcacca actttgaaat ctttcttctt
     1441 ctcataaata acgttcatat agctatcaat ctgataatta cttttcttaa cgaccttttg
     1501 aaatttctta tcaagaaaac atttgttaat tggcatacta ttaccatctg gtgtcatgcc
     1561 atttagctta atcttgctta ttaaatcacc ataatgttgc tcaaaattca ttgtctactt
     1621 atcctgaatg ttccctgaat accgctcata attaccctta caccatcatt ggcttcctta
     1681 tagaagtcaa tagataaggc gacgttgttt cttctcacgt aagccaatta cacgtttggt
     1741 tttcttcttg gtatctttct ttgcggtatc aattaatcgt tctttaccat tcttaccctt
     1801 cactacctta tatcgttcac ttaaaagatt ctttttcagt cctgaaatgt tgccctgctt
     1861 agttaatctt gtagatgaag taggtataaa cttatcaatt gcctttggct gtaccagtac
     1921 ttgataaagg tacttagcct gaatatcttt aaccatgatg gtcgatgtaa ctgaatttct
     1981 cccaaccttt ctatagaaga aaaggatgga acgttgagta aagcccactg ccccacggtc
     2041 aacgctatta ttcaaatcac cctgcattct ttgggatagt agcttggaac gtttaattat
     2101 ttcattctgg aattgattat ttaatctaac tccctgcgtt tgtatatagt tccttatact
     2161 accaatccca tttattccac ttgccatata tttacctcaa ttcaagtact ccaatagttc
     2221 ctgtactatt ccgagtagtc tttttttatc ctttgcattt ggtgatctaa tcttactcat
     2281 cattgccaga ttcaaacatt ccggtttaag catctgatga aggatcagta ttgcctctac
     2341 aatcattacc tctcgcatag ttgcgaagca ccatagaatc gtttttgagt actgttcacc
     2401 attttcaatt ttgtttttaa cgacattgga actactgagg tactcacgcc agccattttc
     2461 ctttgaagtg ggctttagct ttctcgcatc cttaacccgc tgatagattt gcttagcacc
     2521 gacataatta gaaccatcct caaactgaaa gatataaacg aaaccagcat attcaccgga
     2581 taacatttcc tcctcgctcc aatcatccga gtatttccat tcactcatca taaatattcc
     2641 tataaattca ttataggtat ttagtaaatg agcgatttaa aaacacgatt aaaagaatat
     2701 gaaggtacta aagagtatca ggcaaatttg aagtacttca gaaatgggaa attctatcca
     2761 tatgctgata gtttaggatg tagtactatt gtatatggtc acttaattca ggcgggagaa
     2821 gattttagta ctggcattac tgaaagcgaa gcagacaagt tactatctcg cgatttagcc
     2881 aaaacaatta tgcaggtaca gacattaggg ttaaatcttc ccgatgactg gaatgatttt
     2941 attattatca tgaccttcca gttaggtatt ggcggggtga agaaattccg taagatgtta
     3001 gcagccctga aagcacatga ttaccttgaa gcaattaaac aggctaagtg ttctctatgg
     3061 tacaggcaga caccaaaccg tgtgaattcc atgattgcag tacttaaaaa caaataaaga
     3121 aaaggcatag tacgaatgac atactatgcc tttttttatg gtcgttgttt ctgctgaaga
     3181 attgcaataa tcctttcaat cttaacatca agttcatgga tttgtgtttc cagtttactt
     3241 agtgtatctc tcatcttatc ctgatcatcc tcaatacggg aaatactact ttccagtaca
     3301 gcaatggtag tttcaagagt agcaacgcga gttaatagat cattcgtatc ctttgtatta
     3361 tctctaaaga tagtataaag gaatacgcat ccagaaataa caagagctaa aatagcaccg
     3421 atatccatac tgaaatccct ttgttgttat tgttattgtt attgttattg ttattgttat
     3481 tgttattgtt tttgtattaa taccctgtaa atgtcgccaa tgctatctgg ataccattat
     3541 tccccattgc ctggcggtag gttttctggt tctttgaaac gtactgtaga ctgaatgaag
     3601 tactggaatt acgcctgata accagaccag agtacccaat tactgtacca tcatctgata
     3661 tgttccccgg acattgtgac cagcagaacc agggatcagc tactggagac gttaccgtaa
     3721 tggcagtact gagatcggta gccgcaggtg ctgtcaaaaa tcccctgata cgtggcattg
     3781 ttgccgctga tgcagcagac cagattaaag taccttcttc actgaacaca tcaagaaaac
     3841 cagatgtggg ggtagccgtg tgactacttg tcatgaacct gcccatgcca ggctggaaca
     3901 tataggcacc agggaaacac catgccccgt ctttctgtat ctggaaccag tactgtgttg
     3961 tgttcctgtt agctactact gatggcataa cgaaaccgag cgatccacca ttaccgaagg
     4021 cacaggtaaa gcctgtgtag tatcccgtat cagttagatt tcccatggcc tttactgcac
     4081 ccgtcattat cgacttgtta gtactatcta tagtcagtgc acccgctgaa ttatatacct
     4141 cgaaacctgc catcttacgc cgtccactta tatacgttaa aagtgtagtt accagctaac
     4201 ggtgcatatg ttggcaaata aaccgcagta aatgacccat tattacaaaa ggctgaccac
     4261 tcattaaagg tatcactggc gggggcatag tgtacaaacc agcctgtagt tttcatattt
     4321 ggtactgata cagtataggt ctgtgcagaa gtagccttga cagtataagt acccataaaa
     4381 cgtatgttat aatcagtcag atctactact aatacacccg ctgcattcca acattgtaat
     4441 ccctgtgcca tagctcttac tcctgtaaat ccttttcttc attccaattg tttcttttcc
     4501 gctaatactg aatgggttag tactggtact attgaaagta cgttcattag atttcattta
     4561 tgtatccttt tgaaatgagg ttacagttaa taatccctga attatttatt accataaacc
     4621 catacgtaca cgtaacgtat tattattgtc atagatttga ataatattat tactgattac
     4681 cattctacca gtaccacccg ttccattgat taggatcgta cctgctttat tgatttgcca
     4741 gcctgtttta tttgctacgt aattagtact ttgaatagta tttgagattt tggctgtagt
     4801 tatacaacca tcaaccacgt ttaatgtatt cactgagccg gaggccagtt ttagattggt
     4861 aattgccgca tcagcgatgt aaccagtacc gatacttgcc gcctgtatca ttgccgtctt
     4921 caagtatgta gtaccgttga ctatggcaaa tggtgcagta ccacctacag tagcagtgtc
     4981 agtaccggag actatgaact tgttcgcagc gaaatagatt gctgagttgt tactcgttcc
     5041 actactcgca acaagacgca taccagctac cgtgccatta gcctgtactg atagtgagta
     5101 ctgagcatct acagtagatt tatcggcttt agtactcatt gactggttaa cagtggcaat
     5161 ctgtccattc acatcactac gtagctgtga gactgctgta gtctgagctt tgttgttatc
     5221 cgttaccgtc tgggatagtg tctggatact ggccttgtta ccattggcag tactttcaac
     5281 ggtgctaagt ttctgtgata aggaactatt taccccagtg atctgagtac ttaacgtttt
     5341 ctcactggca gtgattttat cattggtacg tgcctctgat gcactgataa cagtactggt
     5401 attcttattg gcaattttga ccgcatcatt aatggtacca gtcaggcgat cttctaaatc
     5461 aagaatcccg ttaattgcct ctgcatcatc ttcggtaaac tggtacttgg agttgatact
     5521 gattgtttgt tctggacaat attgaatatc atcagtacca aacacatcaa agaatcccat
     5581 cttaacctta tactcaccat cagtgatgtt tggtattgaa tcaaattcag gtgcataact
     5641 ggtgaatata cgtgtattag taccattaac aatactgata atcgcacctg cataatcacg
     5701 ttcagtagat ttagtccatg aagcaaacaa attaccaaaa ccacccgtaa agctaacatc
     5761 ctctactaaa ccacactgtt tattctctac ggttattttc acttcctctg aatatgtacc
     5821 atcattaaat ccctgtgcg
//
LOCUS       DAZPEP010000213         5668 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZPEP010000213 DAZPEP010000000
VERSION     DAZPEP010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.215"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..661)
                     /locus_tag="ACHUT9_003989"
     CDS             complement(<1..661)
                     /locus_tag="ACHUT9_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1350347.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
     gene            761..1351
                     /locus_tag="ACHUT9_003990"
     CDS             761..1351
                     /locus_tag="ACHUT9_003990"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFR1350348.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPVWWYSFPAMLKGYI
                     DRVWNNGLAYGDGQKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHUT9_003991"
     CDS             complement(1433..2173)
                     /gene="pflA"
                     /locus_tag="ACHUT9_003991"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFR1350349.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHUT9_003992"
     CDS             complement(2365..4647)
                     /gene="pflB"
                     /locus_tag="ACHUT9_003992"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFR1350350.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHUT9_003993"
     CDS             complement(4702..5559)
                     /gene="focA"
                     /locus_tag="ACHUT9_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFR1350351.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         1460 a         1335 c         1412 g         1461 t
ORIGIN      
        1 ccagaatgat attccctgcg acagcaattt cctgctcctt tgcaaattgc cagtggaaag
       61 gtttgccgct gggataacga aatacaacac attgatgatt aagcagatca tgaggatggc
      121 ggggtttgcc atatcttgca aagtactctg gcgttgctgc tacacataat ttcactggtg
      181 ggccgatcgc cacgctgatc atatcttttt cgacgatgcc actaagtcga acgccagcgt
      241 caaagccctg ttggacgata tcgacaagag agtcgtcagt tgtgagctcg actttaatat
      301 caggatattc acgtgtgaaa ccaaccagta aaggcattaa aaatatacgt gcagctaccc
      361 gggcggcatt gattttgagc gttcctgttg gtgtcagacg aaaatcattc atctcatcta
      421 acatgatctg aatttcgtcg aaagctgggc gcaatcgttc atagagatta gaacccgctt
      481 ccgttaacga tacgcttcgg gttgtccgat tgaataagcg aattttaaga cgttgttcca
      541 gtgttttaat actatggcta atggcggacg aggataagcc taactcatcg cccgctgcac
      601 gaaagctttg attacgggcc acagcaaaga aagtggcaaa gtcagacata ttcatccgca
      661 ttagtgaaaa tccttcatta actcatcctg aatcatcacg tttacatgat tatgccgcct
      721 gcgtaaagtg cgctccagaa cttaacgtgg aggtaaaatt atgcagtctg aacgtattta
      781 tttggtatgg gcccatcctc gtcatgattc attgaccgca catattgctg atgcgatcca
      841 tcagcgggca atggagcgga aaatacaggt gacggaactc gatttatatc ggcgtaattt
      901 caacccggtg atgacgccgg aagatgaacc agactggaag aatatggata aacgttattc
      961 tccagaggtt catcagcttt attcagagct gcttgaacat gacacgttag tggtggtttt
     1021 tcctgtctgg tggtacagct tcccggcaat gctaaaagga tatattgaca gagtatggaa
     1081 taatgggctg gcttatggag atgggcagaa attaccattc aataaagttc gttgggtggc
     1141 gctggttgga ggagacaaag aatcatttgt ccagatgggc tgggaaaaaa atataagcga
     1201 ttatttaaaa aatatgtgca gttatcttgg tattgaagat gccgatgtca ctttcttgtg
     1261 taatacagtg gtattcgatg gggaagaact tcacgcgagc tattatcagt cgttattatc
     1321 tcaggtacgg gatatggtag atgcactata agatgtgtta aaaacgctgt agcagaatga
     1381 agtgcggaat aaaaaagcgg caactcaata aagttgccgc tttacgggga aattagaaca
     1441 ttaccttatg accgtactgc tcaagaatgc ctttcacgcg ttccatggtc tctttcttcg
     1501 gtggtttaac accgtcgagt ttgtactctt cacccattgc cacccatttg tgtttgccca
     1561 gctcgtggta ggggagaagc tcgattttct caacgttgcc catatcacgg gtaaattcac
     1621 cgaggcgatg cgctgaatcg tcatcgtcag accagcctgg gacaacaacg tagcggatcc
     1681 acaccttcac atttttgttc gccagatatt tggcgaactc cagcgtgcgg tgattggaaa
     1741 ctccaaccag attttggtgg atctcgtcgt tcatctgttt gagatcgagc attaccaggt
     1801 cggttacttc cagcagttca tcaatcaccg gatcgtaacg acgaacaaaa ccgttggtgt
     1861 ccagacaggt atgaatgcct tcttttttgc aggcgcggaa ccagtcacga acaaactcag
     1921 cttgtaggat tgcctcaccg ccggatgcgg taacgccgcc gccggaagcg ttcataaagt
     1981 ggcgataggt caccacctct ttcattaaat cttcaacggt aacttcttta ccgccatgcg
     2041 tatcccaggt gtcgcggtta tgacaataca ggcagcgcat caggcagccc tggaaaaagg
     2101 tgataaagcg aatacccggg ccgtctacgg ttccacagga ttcaaaggag tgaatgcgac
     2161 caataactga cattgcggtg tttctccaga tgtggcccat ctgaggccgt gttggtgcgc
     2221 agctcgaagg ctacgtcgag tctgttttgg cagtcacctt agagtataga tagctgacaa
     2281 aaaaggctct cgtgctaaaa aaggccccac tttcgtggag cctttattgt acgcttttta
     2341 ctgtacgatt tcagtcaaat ctaattacat agattgagtg aaggtacgag taataacgtc
     2401 ctgctgctgt tctttagtca gcgagttgaa acgtactgcg tagccagata cacggatggt
     2461 cagctgcgga tatttttccg ggttttccat cgcgtcgagc agcatttcac ggttcatcac
     2521 gttaacgttc aggtgctgac caccttcgat ggatgcttcg tggtggaagt aaccatccat
     2581 cagaccagcc aggttggtct tacgaacttc gtcgtcttta cccagtgcgt tcggaacgat
     2641 agagaaggtg taggagatac catctttagc gtaagcaaac ggcagtttag caacggaagt
     2701 cagagaggct actgcacctt tctggtcacg accgtgcatc gggttagcac ccggtccgaa
     2761 cggcgcgcca gcacgacgac cgtctggggt gttacccgtt ttcttaccat acacaacgtt
     2821 agaagtgatg gtcagaacag actgagtcgg gatagcgtca cggtaggtgt gcagtttctg
     2881 aattttcttc atgaaacgtt ctaccaggtc aacagccagg tcatctacac gcggatcgtt
     2941 gttaccaaac tgcgggtatt cgccttcgat ttcgaagtcg atagccagac cgtcttcgtc
     3001 acgaatcggt ttaactttcg catatttgat tgcagacagg gagtcagcag caacggacag
     3061 accagcgata ccacacgcca tggtgcggat aacgtcacgg tcgtgcagcg ccatcagaga
     3121 ggcttcgtag ctgtacttgt cgtgcatgta gtggatgatg tttagtgcag tgatgtactg
     3181 tttagccagc cagtccatga agtgatccat gcgctccatc acttcatcat agttcaggac
     3241 atcgcctttg atcggttcag acttcggacc aacctgcatt ttcagttttt cgtcaacgcc
     3301 gccgttgatt gcgtacagca tggttttcgc caggtttgca cgcgcaccga agaactgcat
     3361 ttgtttacca acgatcatcg ggcttacgca gcaagcaata gcgtagtcat cgttgttgaa
     3421 gtccggacgc atcaggtcat cgttctcata ctgcagagaa gaggtgtcga tggacacttt
     3481 agcggcgaat ttcttgaagt tcagcggcag tttttcagac cacagaatgg tcatgttcgg
     3541 ttccggagac ggacccatgg tgtacagggt gttcaggaaa cggaagctgt ttttggtaac
     3601 cagggtacga ccgtcgaggc ccataccacc gatagattcg gttgcccaga tcgggtcgcc
     3661 agagaacagt tcatcgtatt ccggagtacg caggaagcga accatacgca gtttcatgac
     3721 caggtggtca accatttcct gcgcttcttg ttcggtgatc ttgccagctt tcaggtcacg
     3781 ttcgatgtac acatccagga aggtggaggt acgaccgaag gacattgcag caccgttctg
     3841 agacttaaca gcagccaggt agccgaagta agtccactgg atagcttcct gagcgttggt
     3901 agccggacca gagatgtcgt agccgtattt cgcagccatt tctttcatct gacccagagc
     3961 gcggtgctgt tcagcgattt cttcgcgcag acggatagtc tgttccaagt ttacgccgtt
     4021 ttccagatca gcctgcagag aagtgaactg tgccagtttg tctttcatca ggtagtcgat
     4081 accgtacagc gcaacgcgac ggtagtcacc gatgatacgg ccacggccat atgcatctgg
     4141 cagaccggtc agaacaccag atttacggca acgcaggatg tccggagtgt aaacgtcgaa
     4201 cacgccctgg ttgtgagttt tacggtattc agtgaagatt tttttgatca tcggatccag
     4261 ttcgcggttg tacgctttgc aggaaccttc gatcattttg ataccaccga acgggataag
     4321 agcacgtttc agcggagctt cagtctgcag accaacgatt ttctcaagct gcttgttgat
     4381 gtagccagcg tcgtgagagg tgatggtgga agcaacagcg gtgtcaaagt caactggcgc
     4441 gtgagtgcgg ttttccagtt taacgccttc cattactttg tcccacaggg tggtggtcgc
     4501 ttcagtagcg ccagccagga aggactcgtc accctcgtac ggagtgtagt ttttctgaat
     4561 gaagtcacgg acgtttactt cattctgcca gtcacctttg gtaaaacctt cccaggctgt
     4621 ggctaacttt tcattaagct cggacatgta acacctacct tcttaagtgg attttttatt
     4681 tactgcgtac ttcgacaacc attaatggtg gtcgttttca cgcaggtaaa tgacccagta
     4741 tgtcaaccca accaacaaac caccaccgat aatgttgccg atcgtaaccg gaatcaggtt
     4801 atcagtgatg aaattcatca cggtcaggtg agaaaaattt tccggtgcag aaccgactgc
     4861 ggtccaaaat tccggggatg cgaagtcgcg gattacaata cccatcggga tcataaacat
     4921 gtttgcgata ctgtgctcaa aaccgctggc aacaaacatc gcgaccggca gcaccataat
     4981 gaacgctttg tccatcaggc tgcggccaga ataactcatc catactgcca gacataccat
     5041 caggtttgcc aggataccaa gacagacggc ctcaataaaa gtatggtgca ctttgtggtc
     5101 ggcggtttgt aggacgttta gtccccattg accatttgcg gtcatatact cgccggaaag
     5161 ccacattaaa agtacaaaca gcagtgcgcc gaccaggttg ccaaaataga catttagcca
     5221 gtttttcgcc aactgacccc aggtgatgcg cccactcgcc ttagcaacaa caatcaacac
     5281 ggtggaagta aagagatcgg ctccgcagac aacacaaaga atcagcccca gagagaagca
     5341 aatgccgcca accagttttg ccatgccgaa gggcattgtg cctgtgccag tggttgctgt
     5401 gatatagaag acgaatgcga ttgagatgaa aacaccggcg gtaatcgcca gatagaaagt
     5461 cttaagcgga tgtttcgttg ctttatagac acccgcctct tcggccactt tggccattgc
     5521 agcaggaagt aaaagatcaa aagggttgtc agctttcaca ctaactctct ctttattaag
     5581 tcggcgacga gatactaaca aagcattata gatgagaaat tgatatagat catatctcgc
     5641 ctggcttata ggcccgtaac tcgcatgg
//
LOCUS       DAZPEP010000214         5640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZPEP010000214 DAZPEP010000000
VERSION     DAZPEP010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.216"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUT9_003994"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="ACHUT9_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFR1350352.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="ACHUT9_003995"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="ACHUT9_003995"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFR1350353.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUT9_003996"
     CDS             1882..2613
                     /gene="yfiH"
                     /locus_tag="ACHUT9_003996"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFR1350354.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUT9_003997"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="ACHUT9_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFR1350355.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1352 c         1529 g         1358 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAZPEP010000215         5619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZPEP010000215 DAZPEP010000000
VERSION     DAZPEP010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.217"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..512)
                     /locus_tag="ACHUT9_003998"
     CDS             complement(<1..512)
                     /locus_tag="ACHUT9_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFR1350356.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(758..1210)
                     /locus_tag="ACHUT9_003999"
     CDS             complement(758..1210)
                     /locus_tag="ACHUT9_003999"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1350357.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1207..2262)
                     /gene="dinB"
                     /locus_tag="ACHUT9_004000"
     CDS             complement(1207..2262)
                     /gene="dinB"
                     /locus_tag="ACHUT9_004000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFR1350358.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2333..3118)
                     /gene="lafU"
                     /locus_tag="ACHUT9_004001"
     CDS             complement(2333..3118)
                     /gene="lafU"
                     /locus_tag="ACHUT9_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFR1350359.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3090..4802
                     /locus_tag="ACHUT9_004002"
                     /pseudo
     CDS             3090..4802
                     /locus_tag="ACHUT9_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4925..5422)
                     /gene="rayT"
                     /locus_tag="ACHUT9_004003"
     CDS             complement(4925..5422)
                     /gene="rayT"
                     /locus_tag="ACHUT9_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFR1350360.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5493..>5619)
                     /locus_tag="ACHUT9_004004"
     CDS             complement(5493..>5619)
                     /locus_tag="ACHUT9_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1350361.1"
                     /translation="HIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1339 a         1467 c         1460 g         1353 t
ORIGIN      
        1 gagccgctat gaaccaacag ttgcagatgt tgtgcatcca gacctgccag tgcaaacagt
       61 tcagcgtcga tgatttgatc aacttgttgc agttcagcga aatgattgcc gccaccgata
      121 gagccaagcg agctgcgcca cggatgctgt gcaaatgctg acggcaggtt ttcctccagc
      181 cagctttctt cagcaacgtc atccaaagct gataatcgct tttcaaactt atcggcgttg
      241 tatttgcgag cgagaatatc tgtttgccat agcgccatgc cgcagccgat atcattgccg
      301 accagtgctg ggtaaaaacg gccaacagag aagaacgctg cgccaatcgg gtagccgcgt
      361 ccgggatgta aatctggcat ccccactacg cgctgcatgt tgggtaaatt tgccgtggtg
      421 tgtaattgtt ggatcgctaa actctcgatc cacaggtcat cagatgcgat ggtaaatacc
      481 gcatcggata agggacgaat atatttgccc atgtaccatt ccgtaaatag actcgtcata
      541 cttcaagttg catgtgctgc gactcgaatt attgagagta acaattaaaa aatcaggaaa
      601 tggcagacga atccagggca agaaaaaaag aattaatcct ggaaaggtct gcaaagagtc
      661 attgataaag gatttgtcat gatttacctc cttgcagttt gtggaataag aaaacgcgca
      721 catactaggc gggcgttatt ttcattgcaa gctggattta atgttgcggt ttatattgca
      781 tataaaaatt agtaaaccac gctccccggc attcaacgtg ctgctgctta actgtctgaa
      841 aaccataacg ttcaaaaaag ggttttgcgg ttatgctggc atctaccgta agttcggatt
      901 cagacttaat aaatggtttt aacaaggcgc tggcaacccc gcggcgggtg tattcagggt
      961 caacaaataa catatcgata taatgttcaa tgcgggaaat aaaaccaacc ggttgtgcat
     1021 taatgaccgc aacccacact tgtgatttcg cgagtttctc cttccagcga gattcgtcaa
     1081 tctgcgccca ggcggcaatt tgttgtggag aataatgctg actggcggtc atcgtaaccg
     1141 ctctaatgaa aatagcgcat agttgctgaa aatcgccagg ctgatagttt cttatttgta
     1201 tgttattcat aatcccagca ccagttgtct ttccatttgc ggatcaagca acgtcacatg
     1261 cagccccacc agtcgcacac cgcgcccgcc gcggcgttca tcccaggttt tacgcgcggt
     1321 ggcgattaag tcagctttat tcagccgcgg ccagacgtgc tcctgagtgg tttgctgaaa
     1381 atcgtcgaac tttaatttca ccccctggcg ggcaatcagt aaatcaggtt ttacctttgc
     1441 cagacggcgt tcaagttccg gatacagccg ctcgataatc gcttcacatt cagaccagtg
     1501 gtggatatct tccgccatcg tgcgttccac gccgacggat tttcgcaacc gttcgctgtt
     1561 aacgtcgcgc tcgtcaatcc cctgactacg ctcccacaaa atgcggccaa atttgccaaa
     1621 gcgtttaagc agcatcacca gatcacactt ttgtacatca ccgcaggttc gtagccccat
     1681 cgcttccagt tttgccgccg agactttgcc gacgccgggg atttttgcca gtggtaaggt
     1741 ttgtaaaaat gccggaactt ctgccggcgt aatcacaaac tggccgttgg gtttattcat
     1801 gtcggaggcg attttggcga gaaactttac gggtgccacg cccgcagacg ccgtcagttg
     1861 cagctcgttg aaaatcgtct ggcggatttc ctgggcgatg agggtcgcag aaccgtggca
     1921 atggacgcta tcggtgacgt cgagataagc ctcatccagt gacaacggtt caatacgcga
     1981 ggtgtagcgc gagaagattt cgcggatatg atttgaggct tctttgtagg cgtcaaagcg
     2041 ccccggaagc aaggtgagat gcgggcataa tttgagcgcc atccctgtcg gcatagcgct
     2101 acgtacgcca aatttacgcg cgggataatt ggcggtactg atcacccccc gacgttcgcg
     2161 gctgccgcca atagcaatag ggatatcgcg cagggcggga ttgtcgcgca tctccaccgc
     2221 tgcgaaaaag cagtccatat ccacatgaat gattttacgc attgctcacc tctcaacact
     2281 ggtaaagtat acagtgattt cagagtttga gaaatgcgta aagattcagc atttaacgcg
     2341 ttcgctgcga aagcacctgc tgcttatcca gcttttgcac cagcggctgc accactttat
     2401 ccccatgctg accaaagtat tgatacagcg tatcggacgc acttttggtc agcaccataa
     2461 tctcaatgcg ccggttgccc gcgctttgcg gatttttgga atccagcagc atctggtccg
     2521 ccattgcgct tacctgcatc actttatctt ccggcattcc ggcctcttcc agcacccgac
     2581 gagccgaaag cgcgcgatcg cccgaaaggt tccagttgtt gtagatattg tttttgtaag
     2641 ccatcgcatc ggtatgcccg gtaataataa ttttgttatc gagcgagtcg aaaactggcg
     2701 cgagctccac cagcagcgtt ttaaagaacg gcataatctg cgcgctgccg cgttcgaaca
     2761 tattgcgatt ctggtcgtct ttaatcagca cgcgtaatcc ctggggaaca atctccatct
     2821 ccagattcgc ttccatatgc gcatcacggg caatggtggt gatactggtt gccagctcgc
     2881 caagctcggt ggcggatttc ttcttcagca gcgccgtttt ctcattgacg tcgcgcgctt
     2941 ttttctccgt ttcttccggc accgccacgg tggcaggttt tggcggatga gacgggctga
     3001 ttttatttaa cggcgacagc ccgccaccgt taaaaatcga ctgcccgtgc agcgcggcga
     3061 taatgctttc acgctcggat ttactgacgc tgttgacaat caactttatt gtcgtcacca
     3121 aaggggccga gcgtatctcc gaggtttctg cccgctttac cctggacgcg atgcccggta
     3181 agcagatggc gattgacgcc gatcttaatg ccgggttgat caatcaggcg caggcacaaa
     3241 cccgacgtaa agatgttgcc agcgaggccg atttctacgg cgcgatggac ggggcatcga
     3301 aatttgtgcg cggggatgct atcgccggga tgatgattct ggcgatcaac ctgatcggcg
     3361 gcgtctgtat cgggatcttc aaatacaacc tgagcgccga cgccgccttc cagcagtatg
     3421 tgctgatgac catcggcgac gggctggtgg cgcaaatccc ttccctgcta ctctccaccg
     3481 cggcggcgat tatcgtcacc cgcgtcagcg acaacggcga tatcgcccat gacgtgcgta
     3541 accagttgct ggcaagccca tcggtgctct acaccgctac cggaattatg ttcgtactgg
     3601 cggtagtgcc gggaatgcca catctgccgt ttttgctgtt cagcgccctg cttggcttta
     3661 ccggctggcg gatgagcaaa cgcccgcagg cggcggaagc ggaagagaaa agtctcgaaa
     3721 cgctgacccg caccatcacc gaaaccagcg agcaacaggt cagttgggaa accattccgc
     3781 tgatcgagcc catcagctta agcctcggct acaagctggt ggcgctggtg gacaaagccc
     3841 agggcaaccc gctcacccag cgtattcgcg gcgtgcgaca ggtgatatcc gacggcaacg
     3901 gcgtgctgct gccggagatc cgcattcggg aaaacttccg ccttaagccc agccagtacg
     3961 ctattttcat caacggcatt aaggctgatg aagcggatat tccggcggat aaactgatgg
     4021 cgctgccctc cagcgaaacc tacggcgaga tagacggcgt gctggggaac gacccggcgt
     4081 acgggatgcc ggtgacctgg atccagcctg cgcagaaagc gaaggcgctg aatatggggt
     4141 atcaggtgat cgacagcgcc agcgtgattg ctacccatgt aaataagatt gtgcgcagct
     4201 atattcctga tttatttaac tatgacgata ttacgcagtt gcataaccgt ttgtcgtcga
     4261 tggcaccgcg cctggcggag gatttaagcg cggtgctcaa ttacagccag ttgctgaaag
     4321 tgtaccgggc gctgctgacc gaaggcgttt ccctgcgcga tatcgtcacc atcgccaccg
     4381 tgctggtcgc cagtagcgcg gtgaccaaag atcatattct gctggcagcc gatgtgcgcc
     4441 tggcgctgcg gcgcagcatt acccatccgt tcgttcgcaa gcaggagctg acggtgtata
     4501 ccctgaataa tgagctggaa aatctgctga ctaacctagt gaatcaggcg caacagggcg
     4561 gtaaagtgat gctcgacagc gtgccagtgg acccgaatat gctcaaccag ttccagagca
     4621 cgatgccaca ggtgaaagag cagatgaaag cggcggggaa agacccggtg ctgctggtgc
     4681 caccgcagct gcgccctttg ctggcgcgtt atgcaaggtt gtttgcgccg gggctgcatg
     4741 tgctgtcgta taacgaagtg ccggacgagc tggagttgaa gattatgggg gcgttgatgt
     4801 aagtatccgg cattggtgca caactgccgg atgcggcgta aacgccttat ccggcctacc
     4861 gttcaggcac aagtttgtag gcatgataag acgcgccagc gtcgcatcag gcatctgcgc
     4921 ataattataa aatgatacgc tcgccagcat ttatatccgt tacgtccccc gcccaatcga
     4981 tgggatataa ccctcgcgcg acatcgcgat ggaacgttga gaatggccaa tcactcactt
     5041 gctttaccca accatgcttt actggattta tataaatata atcaacatga tgcctataat
     5101 ctttggtatt gcggatggcg tgctcccaaa aacgtggttg ccagatattt ttcaatccac
     5161 aagcatgggt aaattgcttt ttaatttccc gccagcgaga ggaaaaatca tcatcgcctt
     5221 caggtaatgt ccatatgcaa tgcatatgct ctggcaaaac gacccaggcg ttgatttcaa
     5281 aaggcctgtc tcgcttaact ttaataatgg cgttacggag catctggtac tgggtggtca
     5341 agagttggct tcgacgattg cgtaaattca ccgtgaaaaa ccaggttccc cccttgatgt
     5401 aataacgacg atattcagac atggcgactc ctttttctca acggtaagaa gaaaaggaaa
     5461 ggtcggcaat gaggtgatgg aacagggcaa gattacgaaa gcccgctccc ctcaaggact
     5521 gacgccagat agtttctgtc catggctgct tttcgcatct tacgtcttaa ccctgccttg
     5581 aataccttat cattcgtcaa aatattaata gcgatgtgc
//
LOCUS       DAZPEP010000216         5613 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZPEP010000216 DAZPEP010000000
VERSION     DAZPEP010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.218"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(104..418)
                     /locus_tag="ACHUT9_004005"
     CDS             complement(104..418)
                     /locus_tag="ACHUT9_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFR1350362.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
     gene            complement(423..638)
                     /locus_tag="ACHUT9_004006"
     CDS             complement(423..638)
                     /locus_tag="ACHUT9_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFR1350363.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(789..2642)
                     /locus_tag="ACHUT9_004007"
     CDS             complement(789..2642)
                     /locus_tag="ACHUT9_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFR1350364.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCCGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            3397..3513
                     /locus_tag="ACHUT9_004008"
                     /pseudo
     CDS             3397..3513
                     /locus_tag="ACHUT9_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3618..3694)
                     /locus_tag="ACHUT9_004009"
     tRNA            complement(3618..3694)
                     /locus_tag="ACHUT9_004009"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3658..3660),aa:Arg,seq:tct)
     gene            complement(3708..3784)
                     /locus_tag="ACHUT9_004010"
     tRNA            complement(3708..3784)
                     /locus_tag="ACHUT9_004010"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3748..3750),aa:Arg,seq:tcg)
     gene            complement(3792..3867)
                     /locus_tag="ACHUT9_004011"
     tRNA            complement(3792..3867)
                     /locus_tag="ACHUT9_004011"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ile,seq:cat)
     gene            complement(3908..4966)
                     /locus_tag="ACHUT9_004012"
     CDS             complement(3908..4966)
                     /locus_tag="ACHUT9_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFR1350365.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(5074..5314)
                     /locus_tag="ACHUT9_004013"
                     /pseudo
     CDS             complement(5074..5314)
                     /locus_tag="ACHUT9_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5539..>5613)
                     /locus_tag="ACHUT9_004014"
     CDS             complement(5539..>5613)
                     /locus_tag="ACHUT9_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1350366.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT         1338 a         1583 c         1321 g         1371 t
ORIGIN      
        1 cagcgccaga acagcagctg acaggccgta tttgattttt gcgctcatgg atatttatca
       61 ggatgctacc aatgaaagat actggaaagc caactgcaaa aagctaacaa cccgtaatcg
      121 agttatcaga actgttaatt tttatggtat accgcgcctc tgaacagggg cgcgtttctg
      181 gcaacagctc gtccccttca cataacccgg cagcaacatc caagaagacc tgtctgatgc
      241 tccttctggc tgctgcctca taaaactcca gcgcggcacc ttcaacacgg tccagcgaga
      301 tgtccaggtc aaaaatttca ccgtcaaagc gttttttgtc ccgtaacgct aaagttaccg
      361 taactttatt ctcaaaattg cggatccctt tcacaatcag ttcatagttt tgagtcattg
      421 aattactctc cccgtgcagc cttacgacgg tcctctctga ttttgaaata caggttagtc
      481 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
      541 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      601 tatgacacac ctgttgtgat tttttccatc tggtacatac cccgtctccc gttatccgga
      661 agctgacaac aataaaaaaa gccaccagtt aagtactgat ggctctgata actcatgcag
      721 gcatctcaga cgacccactg acactaccgg tgagtttaac gataccttcc atttggctgg
      781 ctcacttttt atgatgatgc cggtgcattt atctccagca ccagactttc tatctcaacg
      841 ccatacgctg catttttggt aatatccgtc agcgtcagcg cattcagccc cagtgtcaga
      901 ctgtctttta tgacctggaa tgccgggcca gccactccat tcagtttcgg agtaaccgtg
      961 gcactgccgg cggtgaacac cagctccagc gtcagccagt cgttactgta attcccgaac
     1021 tcgcccaact ttgtgtttcc tgctttcttg tgatgcatca gattcagttt gccgtctgtg
     1081 gtctgggtga agaacgacat caggaacggg ttaccagtcc cggtcatcgc cacgacgtca
     1141 ggtaacgcta catcggtata cagataaatt cccagaccga actggttgtt ggtcagtgcg
     1201 cctgacagtc gaaacttaca gctcagtctg ccaccccgtg tcagcaggga gactgcgtca
     1261 tccaccgggc gcgtcaggga ccaggcttta ttgctctgct tggtgatctt aaatacacca
     1321 tctgacaact gaactccgcc attcttaatg ctccagccct gcgcagcagc gtctccggct
     1381 gtcggcaaca gggagattgt gcgtatggat gcatcttcag acggccccga tggcgtgtcg
     1441 ccgccgggcg agggtttgat ttccggtgcc ttaccactaa tgaaggctaa ggtgcgaccg
     1501 gctacgttca gaatagcagt tgccatacga tcgggaataa tgccacgacg cgcccatgag
     1561 ctgaaatgcg tcgggcgatt tgatgatacc cagtttttgt tcgttcggga tgccgaaccg
     1621 taataaccag acccggcaat atcaggatct tctgacgggt tgtttgtcgg tgtattaact
     1681 ccgctaccat cggtcataaa gggaacaaaa taaatctgct gggattcttt acctttatat
     1741 gcaccatata ccacttcata ttgcgtaccg tgttcttgtt tccacgcgta tgtcgtgtcg
     1801 ccacaaatcc aggggactga tgccggactt ccaccgtgac actgcgccgc cagcccggca
     1861 aggtcagcac ggaactgctg taccattgca agaaatgctg ctggctgctg ggcgtaactg
     1921 gcattcgtca tatcgaattc cccctgcatc cagcatatcg ccagcaaaac gtttttcggg
     1981 tttttctgca atgctgcctt cgtgcggaaa agcagatcct gatataacgg cttacccact
     2041 ccccagcgag ccgaatcctg actggccccc gtggactcgc tgaatgtccc ctccgtgccc
     2101 tgggtgaatg ccgaaccacc acaacagcat ggtaccagca ggatccccgc attattaggg
     2161 atatacggaa gcagtttttt ggcaatatgt aagccctgtc cgacacagcc gtactgccct
     2221 ttgctcaggt cagcccgggg atggttaatc gtactcatat cctgaacatc atgcagacaa
     2281 tggtcagcag gaatgatgtc gttaaatacg catacttcac caccgggagt cactgtgtta
     2341 cgacgggcca gttgcttaat gcgcggatgg ggcgcatcgt atgaatccgg aagcggaaga
     2401 ccttcaccgt aagccatggc attggattgc ccggccagta cgatgacgta gtaccactcc
     2461 ggctcagttg caccactgac gaccacatca ccttctgctg caatcgcctg catcagggta
     2521 taaggggtta tggccaccgg actaccaaac ggctgccagc cctccttcag tttttgtgtc
     2581 agtcgtttcg caaggtctga cggcgatgcc gccctgacca cgtcatagtg tttaaatgcc
     2641 atgaatcctc ccggccggga taatattgtg agtaaaataa ggagcgggct gaagtccgga
     2701 agttacagga caatggcaga agagagacga cagcccgcaa ttcgaaaaag accgcgcagt
     2761 tgcgcagagt gattactatg gggtattatt cgccagctga aatattactt cacgttttat
     2821 tgtttattcc ttgccgcccg cgtctcccag cgcgggcttt ttttgcccac aagaaaaccc
     2881 ctccggagag gggctaaagc cgcgtatctg tatcatcatg cacatggtgc cgggtgcctc
     2941 ccggtgagtt cagcccggtg ccactaaacc cgcgtcattc tcgttttgat aatcagagat
     3001 tataccgtca ccagtcgccc ctccgctcag ggggattcac catgcgaaat ttttttaaca
     3061 aatgcccagt ctgacaggca actgtcaact tactgaattg tgagcaacat agcatttaac
     3121 ggggaacctg ttttctgcag taaaaaggcc caccggagcg gatgggcctg gaaggatagc
     3181 ggtcatgtga tgccggtttc ccggtaactc agcaccggta tctgagtcaa cgttttctct
     3241 actgggtcat ttccgatacg ccctgcctgc tgacaggctt tcatcacatc tgaaaatata
     3301 gcaccctgac tgatactgta gtacctaagg ttccagaaac tgtgatgtat ccggcacaga
     3361 aaagcccctc cggagagggc tggagagtgg cgctatgtgc cattgcatga tgccgggtgc
     3421 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
     3481 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
     3541 acagacaact gtcaaccgtc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     3601 gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     3661 aggcaattgc tctgtccggc tgagctaaca acgcatgatg cagataatgg accgccatcg
     3721 gggacttgaa ccccgcgcag ccagcttcga aggctggcgc tctttcctga tgagctaatg
     3781 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     3841 tcgctctcac cactgagcta aagggccggg agccgcataa taacgacgcg taattaattc
     3901 ttcaatatca tccgttctgg ctgactaaat cctgtacttc ccgaaccgtc tgctcaaaac
     3961 gttcagtctc cagctcaacg ccaattgcac gacgcccgag cgccagtgcc gctttcactg
     4021 tcgaacccaa ccccatgaaa aaatctgcaa tcaggtcacc cggacgactg ctagcgctga
     4081 ttatctgctg cagcatttct gccggttttt cgcacggatg tttcccggga tagaactgca
     4141 ccggtttatg cgtccacaca tcggtatacg gcacctgcac cgtcacgcca aaataccgcc
     4201 gcagatgttt atattcactc agcagctccg catactgccg gttcagtgag gtatacgtat
     4261 ccaccagctg gtggtggagc ttttccagtt caccacgccg atgcttctct tctgccaccc
     4321 gggcaaacag cgcctgtaat ttcagataat cgctttcatt cggcaactgc cactgaccgg
     4381 cactgaacca gtgcgacacc atgtttttct ttcctgtggc atctgcaatc tgttttgccg
     4441 ttatccccag ggcagctcgc gcatcacgaa agtaagaaat cagcggggcc atcacatgct
     4501 gtttcagtgc actgcccttc gccgcatacc cggcatcttt cggacgatac ggcccctgat
     4561 aatgttccgc gaacagaatg cgctctgtgg cggggaaata cgcccgcagg ctttccttgt
     4621 tgcatccgtt ccagcgtccg gacggcttcg cccagataat atggttcagc acactgaagc
     4681 gttcacgcat catgatttcg atatcagatg ccaggcgatg accacagaac aggtaaagac
     4741 ttccggcagg tttcagcacc cgccagaact gcgccagaca ctggtccagc cacttcaggt
     4801 aatcatcgtc gcccttccac tggttatccc agccctcagg cttcacttta aagtacggcg
     4861 ggtccgtgac tatcaggtca acagaatttt cgggtaacga ccggataaat tccaggcagt
     4921 cggcgttgat taactcacaa ctggatattt ttacagtatt aagcatggat cattaagcct
     4981 gtctctgata ggctcattct gcttttgcgc aaagcagtgg gcctgaggtt tgcttgtgaa
     5041 cccaacgcat gagcagatgg ctggtgggtg cccctaacac ccaccagccg cccatttacc
     5101 acaaataaaa aagccttcac tgcggaaggc gtctgtaaca accgaactga tagtctgcca
     5161 gacccgccat aaccagctgg gtcagtatta actggcagcg ttcgcgtgaa aggtaagtat
     5221 tctgcgctat ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc
     5281 tggcggtttc tgtcatatcc tgctgtttta gcatgtcttt tccttttccg gttaacgtga
     5341 cacaccaata actcttgtcg aaaaagccag caagctgaaa gacaggtatt caccgccacc
     5401 agcgcgttta ctgtactgac gcgatttcag tcataaaaaa cccgctcggc ggcgggttgt
     5461 agaaactctt ctaacgtcag gcataaaacg cccatcgtta tgacgaattt accacagatt
     5521 ccggaaaaat caaccttgtt acctagttac cttttttaac tgccgctcag cccatgcttc
     5581 ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPEP010000217         5541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZPEP010000217 DAZPEP010000000
VERSION     DAZPEP010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.219"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1369)
                     /locus_tag="ACHUT9_004015"
     CDS             complement(<1..1369)
                     /locus_tag="ACHUT9_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFR1350367.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWL"
     gene            complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHUT9_004016"
     CDS             complement(1576..1860)
                     /gene="ihfB"
                     /locus_tag="ACHUT9_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFR1350368.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHUT9_004017"
     CDS             complement(2020..3693)
                     /gene="rpsA"
                     /locus_tag="ACHUT9_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFR1350369.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHUT9_004018"
     CDS             complement(3804..4487)
                     /gene="cmk"
                     /locus_tag="ACHUT9_004018"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFR1350370.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHUT9_004019"
     CDS             complement(4660..5424)
                     /gene="ycaL"
                     /locus_tag="ACHUT9_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFR1350371.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         1410 a         1450 c         1304 g         1377 t
ORIGIN      
        1 atagccattg ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc
       61 agaaaagtaa agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg
      121 gcccgcttaa atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca
      181 agggaattgc taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga
      241 tttgcacggg cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg
      301 cacgcaatac cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca
      361 gtgccgcgac cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca
      421 tcagcaaaat tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac
      481 ttaacttaag cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca
      541 ttcccgtcaa ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc
      601 agtggatcca gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca
      661 acaccgcaaa agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc
      721 gcatgatatt tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa
      781 taaccgcatt ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac
      841 gcaggctaca attcggttca ataacacttg cctgcagaaa gcggccggtg agcggctgat
      901 gctgggcaat ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg
      961 cacgaacttt gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt
     1021 cgccatacag cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt
     1081 gaccgtaatg agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg
     1141 cgccagttaa ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga
     1201 aaaacagcaa cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc
     1261 aggcgaagag agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca
     1321 acggaaaaat tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc
     1381 tgtttatgga aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc
     1441 gaggcacatt cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag
     1501 cttccagttc ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca
     1561 agtttgagta aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt
     1621 aaagtgagga acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc
     1681 ggtacgtggt gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc
     1741 gccctgcgca agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt
     1801 cttggcggga atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat
     1861 gattcctccg gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc
     1921 ggccgtagcc gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact
     1981 caacaaactt cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt
     2041 gaaagcttca gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc
     2101 gattgcatct ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt
     2161 tttacgatca acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt
     2221 agcgtcttca acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc
     2281 agccagttct acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc
     2341 gcctttcttg ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc
     2401 cagggagata cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc
     2461 ttttttgtat tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg
     2521 aaccaggccg tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt
     2581 gattttacct tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt
     2641 agctttgcac tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac
     2701 cataacttcc actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt
     2761 ccaatccatt tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca
     2821 gccgtagtca gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt
     2881 agcgatagct acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg
     2941 ttcgcggtcg aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct
     3001 cggatgctta acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc
     3061 cagatcaacg aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc
     3121 ttcctgcagg ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc
     3181 acgacgagaa acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag
     3241 ctctttgcct tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg
     3301 caggaacgca cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat
     3361 aacaccggta acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg
     3421 acgtttagct ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag
     3481 agcaacgtca acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg
     3541 ctcagccggg atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc
     3601 gatagcaaca acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga
     3661 ctcttcaaag agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg
     3721 tccacctggc tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac
     3781 gggggtactg caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc
     3841 gctttttcaa tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggct
     3901 gccggaacca gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg
     3961 gccaaaaggc gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga
     4021 tgcgcacgtt cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc
     4081 accgttccca tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg
     4141 cgacgcaata atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc
     4201 acttcctgag tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg
     4261 ccattggttg acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct
     4321 gacgcaacat caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc
     4381 gaatccagca gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct
     4441 gcaccgcttg ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta
     4501 aaacaggcgt accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta
     4561 acgttgtgtg caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata
     4621 gcgggctggc agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg
     4681 atcacggatg tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt
     4741 gcgaccgcca tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat
     4801 gccacgtttc ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga
     4861 gtatttagca ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga
     4921 agccacaccg ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt
     4981 catttcagcc agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc
     5041 aatttcgttg tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc
     5101 catcgcccat gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt
     5161 aatattgtta cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt
     5221 acttgcgact tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc
     5281 atcggcatcg cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt
     5341 ggtatcaata ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc
     5401 cagcagtaat ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta
     5461 tgtatcaaca acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc
     5521 cgaaaaaaaa cgccgacaat g
//
LOCUS       DAZPEP010000218         5527 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZPEP010000218 DAZPEP010000000
VERSION     DAZPEP010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5527
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.220"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="ACHUT9_004020"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="ACHUT9_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFR1350372.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="ACHUT9_004021"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="ACHUT9_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFR1350373.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="ACHUT9_004022"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="ACHUT9_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFR1350374.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUT9_004023"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="ACHUT9_004023"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFR1350375.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUT9_004024"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="ACHUT9_004024"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFR1350376.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..4812
                     /locus_tag="ACHUT9_004025"
     CDS             4447..4812
                     /locus_tag="ACHUT9_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFR1350377.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            4962..5480
                     /locus_tag="ACHUT9_004026"
     CDS             4962..5480
                     /locus_tag="ACHUT9_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFR1350378.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT         1519 a         1390 c         1241 g         1377 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggtgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatgtttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaata
     4801 cgattaaatt aattaaaacc ccacaaataa attaaacata agattttact cattgtgttt
     4861 tatttctcac attgatgacg atcgcatgaa aaaatgatat tttaatgagc attctgaaaa
     4921 aatatcgatg catttcgagc gaagatggtg gggatccctg aatgcgtttt tctcaccgac
     4981 tgttcttact tctgattctc ctgctgacgg gagcaccaat actcgctcag gagccttctg
     5041 acgttgcgaa aaacgtgcgt atgatggttt ccggtattgt cagttacacc cgctggcctg
     5101 cgctatcagg gccaccaaag ttatgtatct tttcatcttc acgttttagt accgccctac
     5161 aagagaatgc cgcgacatct ttgccttatc tgcctgtcat cattcacact caacaagagg
     5221 cgatgatttc aggctgcaat ggtttttatt ttggcaacga gtcaccgaca ttccagatgg
     5281 aattaaccga acaatatccg tcaaaggcgt tattattaat tgccgaacaa aataccgagt
     5341 gcattattgg tagcgccttt tgtctgatca tccacaataa tgacgtcaga tttgccgtaa
     5401 acctggatgc cttatcgcgt agcggtgtaa aggtcaaccc ggatgtctta atgctcgcac
     5461 ggaagaaaaa tgatggataa cgataattct cttaataagc gccccacgtt taaaagagca
     5521 ttacgca
//
LOCUS       DAZPEP010000219         5455 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZPEP010000219 DAZPEP010000000
VERSION     DAZPEP010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.221"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1334)
                     /locus_tag="ACHUT9_004027"
     CDS             complement(<1..1334)
                     /locus_tag="ACHUT9_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFR1350379.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
     gene            complement(1380..2138)
                     /locus_tag="ACHUT9_004028"
     CDS             complement(1380..2138)
                     /locus_tag="ACHUT9_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350380.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            complement(2170..2883)
                     /locus_tag="ACHUT9_004029"
     CDS             complement(2170..2883)
                     /locus_tag="ACHUT9_004029"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1350381.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3057..3749
                     /locus_tag="ACHUT9_004030"
     CDS             3057..3749
                     /locus_tag="ACHUT9_004030"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFR1350382.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPRPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHUT9_004031"
     CDS             complement(3798..5297)
                     /gene="pitB"
                     /locus_tag="ACHUT9_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFR1350383.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            5234..>5455
                     /locus_tag="ACHUT9_004032"
     CDS             5234..>5455
                     /locus_tag="ACHUT9_004032"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350384.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIK"
BASE COUNT         1459 a         1422 c         1315 g         1259 t
ORIGIN      
        1 gctgcgttat aaagcaagga atgacgctga aaaaatgcct ttaaagttgg aattaacgag
       61 agcaccacat ccagacattg cccaccgaag taagcaaacg ctgctcccag cacaccaaac
      121 agatgacaga acatgaacag caatacgatg tagccgattg aggcgatggt ttgcgccacg
      181 agaaaagcgc gctgttttcc cgccatcaat agcaatgact cctgcgggaa acccagcatc
      241 gaaatcacaa ttgcgcccag catcacctga atcaggtcat acgcttcgag atacttaacg
      301 ccaaacacca gcgaaatgag cggcttgccg gcgatcaaca ccgcgagcgc caccagaata
      361 ccgattccgc ccgccagtaa accggacttc acacctaaca accacggtct ggtggtgcgc
      421 ggatctaaac gcatcacctc tgggtaaaaa cttttaccca gcaaacccgc tggcgttccg
      481 gcggcgtcga agaatgtcat ggcgatttta aataacccgg cggcagcggg tcctaacacg
      541 atccccacca gcactgtgct gcacgagtta cgcgccgacc agatggagtg ggcaatgttg
      601 gttgaccaga caaaactcca cgcgcctttg atgtgtcggg cagactcaaa cagattcaat
      661 ttgaaggcat tatggatatt tctgcggcgt aattcgcgcg ctgcaaacca ccagtacatg
      721 gtgccgccaa ccagattcga cacgtaccag gcaataacaa aacccgcaaa accaaagtca
      781 aaataccagg ctacgacgct ccccgctgcg cgcagaaaag gtttcgtcgc ctgctgtaca
      841 gcaattaaat cgaagcgatc taccgcacgc agaatgccgg tcggcgtgga ggaagccatt
      901 gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc tttggtcatc cagacctaat
      961 gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa tcgccaccgc gccgctgacg
     1021 atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt gctgcggatt attgttggtc
     1081 aaggctggcg ttccgtactg aaccaccagc tgccatgtct gaaacttaat aaaatcgctg
     1141 atcgacttgg cgtacgattg cacaatcacc agtacgccaa acatggcggg cgtcatccct
     1201 ttaccggcac acgagagcgc caacagcccc agcaaggcgc tcacgacatt actggagcct
     1261 aaccaggcgc tattgcgaat aatggtgcga aacgcgccat ctgcaaacca atgtttgatg
     1321 ttaaaacccg ccaattcagc ctgaccttat cgttgatagt aaaaagtatc ccgccagcct
     1381 taagttaaac ttcggcggtc agaaacgatg gcaaccagag aaaccgcctt ctgtgcctgt
     1441 tccagcactt cgctgtaggg cgctctggaa tcaatctcaa gaatttttgt gccgttatag
     1501 ccaatcttcg acatgacacc gattttgtcc tgcagctcgg catagtcatg gtcaggcttg
     1561 cgggagatgg cagtctcaat atcaatgccc aggcgaataa ttaattccgg gcgatattgc
     1621 gccatttgtt ggtataaacg ccgttcgcgc tgcgccagaa acatgctgat tttcccggtc
     1681 gcacgttcga cgccaatccc cggtccgtcg taataaaagc ccgaaatttc agcctgcggg
     1741 aagcgatcgc tgaccaccag aacgccactt tgcgccagtc gctgaacctt gcgtagattc
     1801 gccattcttc gcagcgagaa gcagtacata atcaccgccg cccatagcgc cggagatttg
     1861 gttttcatgc tttgggtttt cgaggatttg gccgccagtc gccgttccag ccagacgcca
     1921 accaacggta atcgtttgat tttgtcgccg tcttcgcccg agagcagccc cagatagcgc
     1981 cgctcggttt gccagtgctg ttgcaatgat ttcaccagat cggtggtcag cgtggattta
     2041 ccggtgccat cgcacccgac caccgcaatt agccccggaa tgtagctggg ctgcggcgcg
     2101 gttgtactat taacagtttg tgtttgtagt gcatccattc gttaaatcat cccctatcca
     2161 ttttggtgat caggaaagcg cggcgtgaat tgcacgtagt gacgcttgca ggatttcatc
     2221 ggctggttgc cgaccatcca gttcaaggat cctggcgccg ttgaaagtca gttgcggcgt
     2281 aacggcgatt ttttcctgta acgctgccag ttggtggtca ggtttacgcg caaacgcggt
     2341 ttgttcatca atgccaagac gaatcaacaa tacgggcaaa taagatgcca tccattggta
     2401 cagcttcagc tcgcgctgcc ttaacatttt tatccaaccg ttaccgcccg tggtttttgc
     2461 caattgcggg ccatcaaagc gaaaacccgg cacttcaacc tgcgggtagc ggtcggtgat
     2521 gagcagaaag ccttgctggc ttttacacaa cattttgcga aacttgtacg cccgccagca
     2581 ggaaagcaga tagataacca gagcagtaat attgccaggc ggtgttgagg gtttttcgtg
     2641 cacatgtgcc gctttacttc gcagataacg cccaaaaggt gcgccaataa cagggagctg
     2701 tgaaatccat tcgccaatta gcccggacga ttgcccgaga taaatgtgtt ctgttggcat
     2761 tcttgctgcc agttcattta ccaggcttgc cgtgagggtc gatttacctg aaccatcaca
     2821 tcctacaatg gcaataacac gcactggcgt ggaattaatt attgacatat tacgttgatt
     2881 cacgaaaaac ccggcagtaa ataatgtatt gaatattacg tggtccgtgc tcagaatatc
     2941 cgttcaacct tattgtgaca atggttaatt gtgacattga cctgaattac gctttcataa
     3001 aaacatatta accaaataaa tatttttaat ggatatttaa attaaaggat atattcatgc
     3061 agtcaataac acctccatta attgccgtta ttggtagcga tggttcaggc aagtcaacgg
     3121 tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc tgccgaaaga gttcatttag
     3181 gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt accgttgatg ggaaaatcct
     3241 tacataaaac aattgaacga aatcaggtga aaacagcaaa aaaattgcct agaccagttc
     3301 cggcgctggt aattacagcg tttgtcgccc gtcgcttact gcgctttcgt catatgcttg
     3361 cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta tcctcaggac caaattcctg
     3421 gcgcttacga tggtacggtg ttcccaccta acgttgaagg tggtcgtttt gtctcatggc
     3481 tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag ccataagcct gatctggtca
     3541 tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa acccgaccat aaacgggaat
     3601 cgctggcgag gaagattgcc ataacgccac agttaacctt tggtggtgca caactggttg
     3661 atatcgatgc caatcagcca ctggaacagg tgttggttga tgcagaaaaa gcgattacgg
     3721 attttatgac cgcgcgtggt tatcactagt caaaaatgga aatgcccgat cgccaggacc
     3781 gggcattttc aggaaggtta aatcaactgc aatgctatcc agtacagccc accagaaaga
     3841 aaaattgccg ccggtaaagt aaacacccac gccatcagga tgctggttac cgttttacgc
     3901 tgtaacccac cgccgtccac caccatcgtc cctgctaccg cagacgagag gacgtgtgtt
     3961 gtggagacag gcatcccaat ataactggca aggccgatag acactgccgc cgtcatttgt
     4021 gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa tcttctcacc gatggtcatc
     4081 gctacacgac gccagccaat catggtgcca atgccgagcg ccagtgctac cgccatgatg
     4141 atccacacag gagcgtactc aatggtgctt aacatatcgc tgcgcagttt tttcagcagg
     4201 ttctggtctt ctttactgac gcctggcagt ttcgccagct tcgcggaggt atcagagatg
     4261 cacagcataa tgcggcgcag ctggctgcgc tgactcacgc ttaacggctc gtaactttcc
     4321 atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat caaaggtatt tgccggatga
     4381 cagtgaaact ctgttacttg cgtgccatca gtcgatgctg caggcaatgg aggttccatc
     4441 gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt agtgttcgaa gttggtgacg
     4501 gcatcgcggg tacgggtaat ttcatagccg gacgcgttca tattgacgac gaagccagca
     4561 ggggcaatcc ccaccagtac cagcatcacc aggccgatcc ctttttgtcc gtcgttcgcg
     4621 ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg caatacgcgt ccagaatggc
     4681 ggtttacgtt tgcctttttt ctttttgcga tcttccggaa tgcggtgaat acggtcacgc
     4741 tttttcgtcc cgctccagta gcgtcgcagc aggaatatca ggcctcccgc aatgaccagg
     4801 ccgacgatag gggaaacaat cagcgaggag aaaattttgg tcacttcacg caggtttaac
     4861 gcatccatca ccgatgatcc ggttaacagc gcgttggtta aaccgatgcc gataatcgca
     4921 ccaatcaagg tgtgcgaact ggaggccggt aaaccgaaga accacgttcc caggttccag
     4981 ataatcgccg ccagcagcat ggaaaagacc atcgccaggc cgtgggttga ccccatattc
     5041 agcaacaaat cggttggcaa catatggaca atggcatagg caacgctaag tccgcccaat
     5101 aacacgccaa aaaagttaaa aaatgccgcc atcaccacag caagttgtgg ttgcatggca
     5161 cgagtataaa taacggttgc caccgcattc gccgtgtcat gaaaaccatt gattgcttcg
     5221 tagaacaaca caaatgccag agcaagcaat aacaaaagcc ctgtgtatat atcaaggcca
     5281 acaaataaat ttagcatata ttagattacg ccattttgaa tttacgaacg gacgcattat
     5341 cagtgacttt aacggcatgg gcaaagtgaa atatcatttt ttaacgataa aaagagtgtt
     5401 atttcttgtg tatcagacaa taagcattca ttcaaaacct ttagaaataa tcaaa
//
LOCUS       DAZPEP010000220         5436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZPEP010000220 DAZPEP010000000
VERSION     DAZPEP010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.222"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..428
                     /gene="soxR"
                     /locus_tag="ACHUT9_004033"
     CDS             <1..428
                     /gene="soxR"
                     /locus_tag="ACHUT9_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFR1350385.1"
                     /translation="PGEVAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYV
                     AIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDE
                     LDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUT9_004034"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="ACHUT9_004034"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFR1350386.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="ACHUT9_004035"
     CDS             2475..4124
                     /locus_tag="ACHUT9_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFR1350387.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5436)
                     /locus_tag="ACHUT9_004036"
                     /pseudo
     CDS             complement(4278..5436)
                     /locus_tag="ACHUT9_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1194 a         1252 c         1426 g         1564 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc gctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt cgagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atggacggcg acattattta taaaatatac caccttaatg gatacttcct
     5221 tttctttact aaggtaaact cttctcgtaa ttctatcgtc acatttatta tttgcatata
     5281 aagctatatc tataaaatgt ttatgtattt tcctgtccgt ttccttcgtg atttttcctg
     5341 atggtaaact atcataaatt cttttcattt tctgaatcaa acctatcact ttttcatgat
     5401 catccatctt gaaaagagag agggctttat tgtcaa
//
LOCUS       DAZPEP010000221         5374 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZPEP010000221 DAZPEP010000000
VERSION     DAZPEP010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5374
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.223"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUT9_004037"
     CDS             complement(57..1340)
                     /gene="aroA"
                     /locus_tag="ACHUT9_004037"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFR1350388.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUT9_004038"
     CDS             complement(1411..2499)
                     /gene="serC"
                     /locus_tag="ACHUT9_004038"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFR1350389.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2698..3390)
                     /locus_tag="ACHUT9_004039"
     CDS             complement(2698..3390)
                     /locus_tag="ACHUT9_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFR1350390.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUT9_004040"
     CDS             3520..5280
                     /gene="ycaO"
                     /locus_tag="ACHUT9_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFR1350391.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1374 a         1387 c         1269 g         1344 t
ORIGIN      
        1 caatgtgccg acgttttgat aaagaaagat ttggctattt attgcccgtt gttcattcag
       61 gctgcctggc taatccgcgc caactgctcg aaataatccg gaaatgtttt ggccgtacat
      121 ttgggatcaa gaatcgtcac tggtgtatct gacaacgcca ccagcgagaa acacatcgcc
      181 atccggtgat cattgtatgt cgcgatctcg gcaaagttca gtttttccgg tggagtgata
      241 cgaatgtaat cgtgcccctc ttctacttcc gcaccgactt tacgcagttc tgttgccatc
      301 gcaaacaggc gatcggtctc tttaacacgc cagttataga tattgcgcag cgtggtggtg
      361 ccttttgcaa ataacgccgc cgtggcaatg gtcatcgccg catcgggaat atggttcata
      421 tccatatcaa tagcgttcag ttcaccacgc gtgcaggaaa tataatcatc gccccagcaa
      481 atggtcgcgc ccattttttc cagcacatca gcaaagcgaa tatcaccctg catactgtta
      541 cgtccaatac cggtcacttt tacagtgccg cctttgattg ctgctgctgc cagaaagtaa
      601 gaagccgaag atgcatcgcc ttcgaccaaa taagtacccg gagactgata agactgcccg
      661 ccttttacga caaattgttg atagtgctga ttttcaattt caacaccaaa cgtcttcatc
      721 agattgagtg tgatgtcgat ataaggttta gaaaccagat cgcctttaat acgaatcacc
      781 gtatcttccg gcgcaagagg cgcagtcatt aacagtgcgg tgaggaattg gctggaaacg
      841 gagccatcaa cgtcaacgtt gccgccagta aagccgccct gtaaacgcaa cggcggataa
      901 ttttcttgtt ccaggtaagt gatcttcgcc ccgcccaggc gcagcgcatc caccagatga
      961 ccaatcgggc gttctttcat acgcggctca ccggtcagca caatatcatt gctacccaga
     1021 caaagagctg ccgccagcgg acgcattgcc gttccggcgt taccgaggaa caactccagg
     1081 gcaccttctg cgtgtaatgg accgccgtta ccgataattt cgcaacgcgt acgatcggct
     1141 gaaagcgtat agcttacccc taacgctgtt aatgcattca gcatatggcg cacgtcatcg
     1201 ctatccagca gattggttaa tactgttttg ccgtgtgcta atgccgccag caataaagcg
     1261 cggttagaaa cgctcttgga accgggcaga ttaatagtgc catcgacacg agcgatgggt
     1321 tgtaacgtca gggattccat gaactcaact ctctacaaca gaaataaaaa ccccacaggc
     1381 tggctgtggg gattaagcaa aatttcggca ttaaccgtga cggcgttcga actcaaccat
     1441 gaagtctgtc agcgctttaa cgccttccag cggcatggcg ttataaatag aagcgcgcat
     1501 tccgccgacc acacggtgac ctttcagtgc atgaaggcca gcagcaaaag actcttcaag
     1561 gaacaatttg tcaagcgcac tgtccgccaa ctggaacggc acgttcatcc gcgaacggtt
     1621 agctttcgcc acgtcattgc ggtagaaatc gctgttatca atcaccccat atagcagttc
     1681 tgctttttgc tgattgattt tatccatttc agctacaccg ccgttcgctt tcagccattt
     1741 aaagaccaga ccagatagat accaggcaaa tgtcggcggc gtgttaaaca tggagccgtt
     1801 atcgttgagg atggaataat ccagaatcga cggacacgcg atattcgctt tgcccagcaa
     1861 atcttcacga acgatgacga ttgtcaggcc agccgggccg atatttttct gcgcgccagc
     1921 gtaaatcacg ccataacggc tgacgtcaat cggacgggaa agaatggttg aagagaagtc
     1981 ggcggcgacc accacatctt tgccgaagtc tggcgtttcg tcgatggcga taccgtcgat
     2041 ggtttcattc gggcagtaat gcatataagc agcattatca gagagttgcc attcacgcat
     2101 tggcttaacc gcgcgcagac catcaacagt cactttggcg tcaaagacat taggcgtgca
     2161 gtattttttc gcttctttaa tggcacttgc cgcccagtaa ccggcatcaa cataatctgc
     2221 ggtggtttta tcaccgagaa tattcagcgg taccgcagca aactgaccgc gaccaccgcc
     2281 atggcagaat aataccttgt agttggaggg gacattaaga agatcgcgaa aatccttctc
     2341 ggcttcctct gcaacctgaa tgaactcttt gccacggtga ctcacttcca tcaccgacgt
     2401 accaagaccg ttccagtcgc gcagttcctg ttgagcctgt ttaagcacct ctgccggtag
     2461 cattgccgga ccagaactaa aattgaagat ttgagccatt gcccctcacc acgttgcgtt
     2521 gctattaacc cgcggtcaat cgattgcgac cacaggtaat acattattcc tgtggctatc
     2581 ggttttatca ttcagtgaca cacatcgcaa tgagtaaaac cgttgagggc aaaaatgtgg
     2641 cttgcatcac acaaaagaat ctctcggatt attagcaaaa agccgatgtt tttactgtca
     2701 ggtcacccgt tttgcccgac ttgccttcgt ccattctgga tttgcacagc gcggacataa
     2761 ttgtttttcc cccgctttca tatgaatgat ttcactacaa cgaatacagg catagtccgc
     2821 cgactccggc accactttgt aacgctgcgt acaatcactg ggtaaaataa gtaaacccgg
     2881 tttcaccttg tcatcttcaa agaaatagac actgatttgc ccgttggttt cgagaatcgc
     2941 cagacgtacc tgccccagct gctccacgcc acgcaatcgt agctccataa agaactcaaa
     3001 ttccgtcatg ttggagttat tgagtttcga ccaggccagc tcgccatctt caataataac
     3061 aaccggctta ccttccagta gatcttccag tttttcactg tgcgccatca accacattac
     3121 caggcggtat aacaacgcca gagtgataaa gacgataagt accggcacca tcggcacatc
     3181 atcataaaac gccacatctc ccgccgctga acccagcgtg agaatgatta acacttcaaa
     3241 cagcgacatc tgtcgcacac cgcgtcttcc ggtcattttg aggaacaaaa aaaccagtac
     3301 aaaggtataa agactacgta gtgcaacttc gccaaggaaa tcaaaaggca ctttatcaaa
     3361 tgccatacgg tggagatcga acgccttcat ttttatgctc ttatcgtggt ttatccgcta
     3421 aaagcatagt caacagttca tcggcatggc ggcggtgata gcgcctttga gataaaaccc
     3481 gtattattgc gcgctttccg tacgactaaa gtgattttca tgacgcaaac atttatcccc
     3541 ggcaaagatg ccgctctgga agattccatc gctcgcttcc agcaaaaact ttcagacctc
     3601 ggctttcaga ttgaagaggc ctcctggctg aatcctgtgc ctaacgtctg gtctgtacat
     3661 attcgcgaca aagagtgcgc actgtgtttt accaacggta aaggcgcaac caaaaaagcg
     3721 gcgctggctt ctgcacttgg tgaatatttc gagcgtctct caaccaacta cttttttgct
     3781 gatttctggc tgggcgaaac catcgccaac ggtccgttcg tgcattatcc caacgaaaaa
     3841 tggttcccac tgaccgaaaa tgacgatgtg ccagaaggac tactcgatga ccgtctgcgc
     3901 gcgttttatg atccggagaa tgaactgacc ggcagcatgc tgattgacct acaatccggt
     3961 aacgaagatc gtggtatttg cggcctgccg tttacgcgtc agtccgacaa tcagaccgtt
     4021 tatattccga tgaatatcat tggtaacctg tacgtctcca acggtatgtc cgcaggtaat
     4081 acccgcaacg aagcacgcgt tcagggattg tctgaagttt tcgaacgcta cgtgaaaaac
     4141 cgcattattg ctgaaagcat cagcctgcca gagatcccgg cagacgtgct ggcgcgttac
     4201 ccagcagtag ttgaagccat cgaaacgctg gaagcggagg gtttcccaat cttcgcttat
     4261 gacggttcgc ttggcggcca gtatccggtg atttgcgtgg tactgttcaa tcctgctaac
     4321 ggtacctgct ttgcctcttt cggtgcgcat cctgattttg gcgtagcact ggaacgtacc
     4381 gtgaccgagc tgctgcaagg tcgtggcctg aaagatttgg atgtgtttac tccgccaacc
     4441 ttcgatgatg aagaagtcgc tgaacatacc aacctcgaaa cgcactttat cgattccagc
     4501 ggtttaatct cctgggacct gttcaagcag gatgccgatt atccgtttgt ggactggaat
     4561 ttctccggca ccacggaaga agagtttgct acgctgatgg ctatcttcaa caaagaagat
     4621 aaagaagttt atattgccga ttacgagcat ctgggcgttt atgcttgccg tattatcgtg
     4681 cctggcatgt ccgatattta tccggctgaa gatctgtggc tcgcgaataa cagtatgggc
     4741 agccatttac gtgaaacgat tctttcgcta ccaggcagcg agtgggaaaa agaagattac
     4801 ctgaacctca tcgagcaact ggatgaagaa ggttttgatg actttacccg cgtgcgtgag
     4861 ctgttgggtc tggcgaccgg gtcggataac ggttggtaca ccctgcgtat cggtgaatta
     4921 aaagccatgc tggcgctggc tggtggcgat ctggaacagg ctctggtctg gaccgaatgg
     4981 acgatggagt ttaactcatc agtatttagt ccggaacgcg ccaactatta tcgctgcctg
     5041 caaacgttgt tattactggc acaggaagaa gatcgccagc cgctgcaata tctgaatgcg
     5101 tttgttcgca tgtacggcgc agatgccgta gaagccgcca gtgcggcaat gagcggcgaa
     5161 gcggcgtttt acggcctgca accggtagat agcgatctgc acgcgtttgc tgcacatcag
     5221 tcgttgttga aggcctacga aaagctgcag cgcgccaaag cagcattctg ggcaaaataa
     5281 aatcaaatag cctacgcaat gtaggcttaa tgattagtct gagttatatt acggggcgtt
     5341 tttttaatgc cccgctttac atatatttgc atta
//
LOCUS       DAZPEP010000222         5302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZPEP010000222 DAZPEP010000000
VERSION     DAZPEP010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.224"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            325..411
                     /gene="leuL"
                     /locus_tag="ACHUT9_004041"
     CDS             325..411
                     /gene="leuL"
                     /locus_tag="ACHUT9_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFR1350392.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            504..2075
                     /gene="leuA"
                     /locus_tag="ACHUT9_004042"
     CDS             504..2075
                     /gene="leuA"
                     /locus_tag="ACHUT9_004042"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFR1350393.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPLEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2075..3166
                     /gene="leuB"
                     /locus_tag="ACHUT9_004043"
     CDS             2075..3166
                     /gene="leuB"
                     /locus_tag="ACHUT9_004043"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFR1350394.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3169..4569
                     /gene="leuC"
                     /locus_tag="ACHUT9_004044"
     CDS             3169..4569
                     /gene="leuC"
                     /locus_tag="ACHUT9_004044"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFR1350395.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4580..5185
                     /gene="leuD"
                     /locus_tag="ACHUT9_004045"
     CDS             4580..5185
                     /gene="leuD"
                     /locus_tag="ACHUT9_004045"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFR1350396.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5234..>5302)
                     /locus_tag="ACHUT9_004046"
     CDS             complement(5234..>5302)
                     /locus_tag="ACHUT9_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350397.1"
                     /translation="VYWVIAVISVVALFLTAKVKDV"
BASE COUNT         1352 a         1405 c         1398 g         1147 t
ORIGIN      
        1 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
       61 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
      121 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
      181 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
      241 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
      301 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
      361 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
      421 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
      481 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
      541 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
      601 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
      661 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
      721 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
      781 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
      841 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
      901 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
      961 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     1021 gctacaccat gccgcttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     1081 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     1141 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     1201 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     1261 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     1321 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     1381 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     1441 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     1501 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     1561 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     1621 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     1681 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     1741 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     1801 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     1861 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     1921 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     1981 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     2041 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     2101 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     2161 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     2221 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     2281 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     2341 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     2401 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     2461 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     2521 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     2581 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     2641 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     2701 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     2761 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     2821 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     2881 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     2941 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     3001 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     3061 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     3121 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     3181 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     3241 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt cgatggtctg
     3301 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     3361 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     3421 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     3481 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     3541 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     3601 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     3661 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     3721 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     3781 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     3841 gaaatgggcg caaaagccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     3901 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     3961 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     4021 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     4081 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     4141 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     4201 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     4261 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     4321 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     4381 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     4441 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     4501 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     4561 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     4621 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     4681 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     4741 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     4801 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     4861 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     4921 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
     4981 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
     5041 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
     5101 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
     5161 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
     5221 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
     5281 taaccgcaat tacccagtag ac
//
LOCUS       DAZPEP010000223         5159 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZPEP010000223 DAZPEP010000000
VERSION     DAZPEP010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.225"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(182..325)
                     /gene="ypaB"
                     /locus_tag="ACHUT9_004047"
     CDS             complement(182..325)
                     /gene="ypaB"
                     /locus_tag="ACHUT9_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFR1350398.1"
                     /translation="MTLLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            453..2738
                     /gene="nrdA"
                     /locus_tag="ACHUT9_004048"
     CDS             453..2738
                     /gene="nrdA"
                     /locus_tag="ACHUT9_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFR1350399.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2972..4102
                     /gene="nrdB"
                     /locus_tag="ACHUT9_004049"
     CDS             2972..4102
                     /gene="nrdB"
                     /locus_tag="ACHUT9_004049"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFR1350400.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4102..4356
                     /gene="yfaE"
                     /locus_tag="ACHUT9_004050"
     CDS             4102..4356
                     /gene="yfaE"
                     /locus_tag="ACHUT9_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFR1350401.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4410..5059)
                     /gene="inaA"
                     /locus_tag="ACHUT9_004051"
                     /pseudo
     CDS             complement(4410..5059)
                     /gene="inaA"
                     /locus_tag="ACHUT9_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
BASE COUNT         1284 a         1381 c         1260 g         1234 t
ORIGIN      
        1 ttaacaataa ccgaatagaa aacaaccatt tcgacatcaa caatcgctta cattcgctta
       61 tatattgacc acaactgata catcagatta tgtgatgact cgtgcttaga tcaatttttg
      121 caatcattaa caaaaagatt aataaaccat ctatatcaat ttatccaacc tattctgccg
      181 ttcaagaaat cgccgaacag ttatttttaa caaatttttc tcttcccatt gactttcccg
      241 gacaccttgt ctgacctaag gtgcgcgaaa gccacttttt ccttcctgag ttatccacaa
      301 agttatgcac ttgcaagagg gtcattttca cactatcttg cagtgaatcc caaacatacc
      361 ccctatatat agtgttctaa gcagcttccc ttactacagg tagtctgcat gaaactattg
      421 cggaaagaat tccaaaaaca ggtacgacat acatgaatca gaatctgctg gtgacaaagc
      481 gcgacggtag cacagagcgc atcaatctcg acaaaatcca tcgcgttctg gattgggcgg
      541 cagaaggact gcataacgtt tcgatttccc aggtcgagct gcgctcccac attcagtttt
      601 atgacggtat caagacctct gacatccacg aaaccattat caaggctgcc gcagacctga
      661 tctcccgtga tgcgccggat tatcagtatc tcgccgcgcg cctggcgatc ttccacctgc
      721 gtaaaaaagc ctacggccag tttgagccgc ctgcgctgta cgaccacgtg gtgaaaatgg
      781 tcgagatggg caaatacgat aatcatctgc tggaagacta cacggaagaa gagttcaagc
      841 agatggacac gtttatcgat cacgaccgtg atatgacctt ctcttatgct gccgttaagc
      901 agctggaagg caaatatctg gtacagaacc gcgtgaccgg cgaaatctat gagagcgccc
      961 agttccttta tattctggtt gccgcgtgcc tgttctcgaa ctacccgcgt gaaacgcgcc
     1021 tgcaatatgt gaagcgtttt tacgacgcgg tttccacatt taaaatttcg ctgccgacgc
     1081 caatcatgtc cggcgtgcgt accccgactc gtcagttcag ctcctgcgta ctgatcgagt
     1141 gcggtgacag cctggattcc atcaacgcca cctccagcgc gattgttaaa tacgtttccc
     1201 agcgtgccgg gatcggcatc aacgccgggc gtattcgtgc gctgggtagc ccgattcgcg
     1261 gtggtgaagc gttccatacc ggctgcattc cgttctacaa acatttccag acagcggtga
     1321 aatcctgctc tcagggcggt gtgcgcggcg gtgcggcaac gctgttctac ccgatgtggc
     1381 atctggaagt ggaaagcctg ctggtgttga aaaacaaccg tggtgtggaa ggcaaccgcg
     1441 tgcgtcatat ggactacggg gtacaaatca acaaactgat gtatacccgt ctgctgaaag
     1501 gtgaagatat caccctgttc agcccgtccg acgtaccggg gctgtacgac gcgttcttcg
     1561 ccgatcagga agagtttgaa cgtctgtata ccaaatatga gaaagacgac agcatccgca
     1621 agcagcgtgt gaaagccgtt gagctgttct cgctgatgat gcaggaacgt gcgtctaccg
     1681 gtcgtatcta tattcagaac gttgaccact gcaataccca tagcccgttt gatccggcca
     1741 tcgcgccagt gcgtcagtct aacctgtgcc tggagatagc cctgccgacc aaaccgctga
     1801 acgacgtcaa cgacgagaac ggtgaaatcg cgctgtgtac gctgtctgct ttcaacctgg
     1861 gcgcaattaa taacctggat gaactggaag agctggcaat tctggcggtt cgtgcacttg
     1921 acgcgctgct ggattatcag gattacccga tcccggccgc caaacgtgga gcgatgggtc
     1981 gtcgtacgct gggtattggt gtgatcaact tcgcttacta cctggcgaag cacggtaaac
     2041 gctactccga cggcagcgcc aacaacctga cgcataaaac cttcgaagcc attcagtatt
     2101 acctgctgaa agcctctaat gagctggcga aagagcaagg cgcgtgcccg tggtttaacg
     2161 aaaccactta cgcgaaaggg atcctgccga tcgataccta taagaaagat ctggatacca
     2221 tcgctaatga gccgctgcat tacgactggg aagcattgcg tgaatcaatc aaaacgcacg
     2281 gcctgcgtaa ctctacgctt tctgccctga tgccgtccga gacctcttcg cagatctcta
     2341 acgccactaa cggtattgaa ccgccgcgcg gttacgtcag catcaaagcg tcgaaagacg
     2401 gtattttgcg ccaggtggtg ccggactacg agcacctgca cgacgcctat gagctgctgt
     2461 gggaaatgcc gggtaacgat ggctacctgc aactggtagg tatcatgcag aaatttatcg
     2521 atcagtcgat ctctgctaac accaactacg atccgtcacg tttcccgtca ggaaaagtgc
     2581 cgatgcagca gttgctgaaa gacctgctca ccgcctacaa attcggggtc aaaacgctgt
     2641 attatcagaa cacccgtgac ggcgctgaag acgcgcagga cgatctggta ccgtcaatcc
     2701 aggacgatgg ctgcgaaagc ggcgcatgta agatctgata ttgagatgcc ggatgcggcg
     2761 taaacgcctt atccggccta cggctcggtt tgtaggcctg ataagacgcg ccagcgtcgc
     2821 atcaggctcc gggtgccgga tgcagcgtga acgccttatc cggcctacgg ctcggatttg
     2881 taggcctgat aagacgcgcc agcgtcgcat caggcacagg atgcggcgta aaatgcctta
     2941 tccggcatta aactcccaac aggacacact catggcatat accacctttt cacagacgaa
     3001 aaatgatcag ctcaaagaac cgatgttctt tggtcagccg gtcaacgtgg ctcgctacga
     3061 tcagcaaaaa tatgacatct tcgaaaagct gatcgaaaag cagctctctt tcttctggcg
     3121 tccggaagaa gttgacgtct cccgcgaccg tatagattac caggcgctgc cggagcacga
     3181 aaaacacatc tttatcagca acctgaaata tcagacgctg ctggattcca ttcagggtcg
     3241 tagcccgaac gtggcgctat tgccgcttat ttctattccg gaactggaaa cctgggtcga
     3301 aacctgggcg ttctcagaaa cgattcattc ccgttcctat actcatatca ttcgtaatat
     3361 cgttaacgat ccgtctgttg tgtttgacga tatcgtcacc aacgagcaga tccagaaacg
     3421 tgcggaaggg atctccagct attacgatga actgatcgaa atgaccagct actggcacct
     3481 tctgggcgaa ggcacccaca ccgttaacgg taaaaccgta accgttagcc tgcgcgagct
     3541 gaagaaaaaa ctgtatctct gcctgatgag cgttaacgcg ctggaagcga tccgcttcta
     3601 cgtcagcttt gcttgttcct ttgcctttgc agaacgcgaa ctgatggaag gtaacgccaa
     3661 gattattcgc ctgattgccc gcgatgaagc cctgcacctg accggtactc agcatatgct
     3721 gaatctgctg cgcagcggcg cggatgatcc ggagatggcg gaaattgccg aagaatgtaa
     3781 gcaggagtgc tatgacctgt ttgtccaggc ggcacagcaa gagaaagact gggcggatta
     3841 tctgttccgt gacggttcga tgattggtct gaataaagat attctctgcc agtacgttga
     3901 atacatcacc aatatccgca tgcaggcagt cggtttggat ctgccgttcc agacgcgctc
     3961 caacccgatc ccgtggatca acacctggct ggtgtctgat aacgtgcagg ttgctccgca
     4021 ggaagtggaa gtcagttctt atctggtcgg gcagattgac gcggaagtgg acaccgacga
     4081 tttgagtaac ttccagctct gatggcccgc gttaccctgc gcatcactgg cacacaactg
     4141 ctgtgccagg atgaacaccc ttcccttctg gcggcgctgg aatcccacaa tgttgcggtt
     4201 gagtaccagt gccgcgaagg ttactgcggc tcctgtcgca cccgactggt cgcaggtcag
     4261 gtggactgga ttgccgaacc gttagccttt attcagccgg gggaaatttt gccctgttgt
     4321 tgccgggcaa aaggcgatat tgaaatcgag atgtgaattg gttgtagtgc cagatacaac
     4381 gcttatacgt cttatctggc ctacaacgat tacatggcgt agtaatacgc tttcacctgc
     4441 tcccagtccg ctttggggat tggctccaga tattttccag ctggcggaag tcatgattaa
     4501 tcgctttatt gcgacgcaag cggcgacgac ttttctccag gtcaagaaaa ccagcttctg
     4561 ctttaccttc tgttttcaca tagatatggc gaacataaca acagccatgc tgacgattaa
     4621 cgctatgcat tttcttaaac gccagcgcca ctgctttcaa catggcttgc cgtacttcgt
     4681 cagaataggg cgatactgca tgccgggcat accagtcagc aatgctgatg aaccccgcca
     4741 tatcttcagt caccagcaac gcgcgccatt caccctcaat tttcaccgct tcaccaaaaa
     4801 cgatcttcgg cacgatgaca ccggcccgtt ccagttcttt aattaccgca acctcacgga
     4861 caatcgttgg tcggccgaac ggataacgta cggaatgaaa cagatgatgc gtcatgcgct
     4921 ttacatacag ctttttgccg ttgcgctcga cgcattgcac cccgctcata ccattacggc
     4981 gatagttagg ctcttcaacc cagtcgccct ctgttgccca ccagtgatta aattcgtcgt
     5041 actttgctga aactgccata ccctatcgcc tgtcattttt attaacgaca atgactatag
     5101 gtggttacct gaggaaaatc ttaatgaaac gtgtcgtatt aatgaatttc ttttgccgc
//
LOCUS       DAZPEP010000224         5119 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZPEP010000224 DAZPEP010000000
VERSION     DAZPEP010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.226"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1499
                     /locus_tag="ACHUT9_004052"
     CDS             <1..1499
                     /locus_tag="ACHUT9_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFR1350402.1"
                     /translation="DKNAANNSKQTQMVNGTFIYWLNLNTPGNLRGITTRCVLLDEVS
                     SCEITDEGNPIKLAEARTSTFGSDSLVVVSSTPLYKDDLINAEYNLSDKRRYFVTHTC
                     GHEYTFEWEQVAFEFKQLENGRSIPDSTTTRLLCPHCNEGIDEHTRHQMVDNGRWIAT
                     NPDGEPGVVGYQISRMYSPLNTITEMVSKFADALYNFNLQTFYNNELGLPFEDEYQKE
                     LDILQLESLREDEFNLYKIPESTLALCISVDQQGDRLESLLLGFDEKNIYVLGHEFFY
                     SHDCTKIEAPAWKDLDQFCRQDFSTVSGRIVPTLAVFIDAGNGNATDTVKKFTTRWSK
                     YHPIKGSSSTTGELFKTSTQAGYKLQILNVHEQKNTIRRLLNLMLSSEADNAPVKLRF
                     SSTLPSDFFEQLSAEELKPAGGKLVWRLKKGQRRNEALDLICYGMIAIAYAQSKLGTQ
                     PFRKLRDYKAQETTKYEINKVEEPKPETVQKPKRNRRTGMGSNWFGKR"
     gene            1511..2041
                     /locus_tag="ACHUT9_004053"
     CDS             1511..2041
                     /locus_tag="ACHUT9_004053"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350403.1"
                     /translation="MTLLPEKIYMVSNPMDLTVLVPAATILVISFTSSGKSTSLDNLN
                     GSTSKEFTVVLDMSTAQELLFCTQISNGQTSTFTSEVINPLLYTSEYSQLKQMISEID
                     QVIANKIAGGANYSITINNKTLVSESLSSLESMRERYVKRANALFVKMNGGTFSNGGK
                     PIKSITVFRPKPGSTR"
     gene            2041..3549
                     /locus_tag="ACHUT9_004054"
     CDS             2041..3549
                     /locus_tag="ACHUT9_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1350404.1"
                     /translation="MFWNKKNKNSETKPKKIKQERSLKQSTLKRDLQAVRNTSVMNFG
                     FNSNSGSNINFLLMKALPTFRAFSRDAVLKNPIGRKYMNLSVDGVVGSDGLYVKPSVE
                     IDGTEEEINGINLTLEKLFDRWAYDADRFSVDGSLSFDLFQQNAEKVRVQDGECFIRI
                     HNINNTIKLEILDTVRLLQLNNQRLSNGNYISNGIEFDKWHKPVNYYFCKFDPVTYTY
                     ITGDYEVIPAAEICHYFIADQQGQERGLPDMVATSKLMEDLKNFTEAALTAKRVSASS
                     MAFITNNNDTTSTDLLGADEREEVTPVYTEYFEAGFIGELAEGQDIKTVTPTNGVDGI
                     DQFTNELMNQISMGLNITKQALLSDTSNASFSASRLTEKLQQTTFRTRTNVLISKVLK
                     PIYIAWLKNEMLNNNKLNLSFLSFDDLICARYISQKPISLDPVKDIQAELLQLEAGIK
                     SKTQVIAELGGDPVKVLAEVQAEKDKENKNKEVIQDGNEKPEEGTNETPTGD"
     gene            3497..>5119
                     /locus_tag="ACHUT9_004055"
     CDS             3497..>5119
                     /locus_tag="ACHUT9_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFR1350405.1"
                     /translation="MEMKNQKRELTKPLQAINTESRTIEVAFCSETPVEREINGQIYN
                     EILLCGSSNADLTRLNNNGAVLFNHNRDDLIGAVVDARVDADRVGRATLRISSTANDE
                     WEMIQEGVLTHISIGYNINDYQIDGNNIYVTNYEIYEISLVTVPADPTVGVGRSMDSQ
                     LALKSLNKEGETIQEGQMDMDNPENEKESIVDSTEEVNETIEEALVEPSVEYAEEIKQ
                     ELSDEELLEIISTRPDLLNKVHGEEPESINSTEDTERVRELEALGKVLNIDVSEAIET
                     GISISDFKRQLNDIKQNPLHDKEIKEMDNKNLLKDMVRAIKTGDKSALEAYERGINGF
                     VRAAVPSTNTTTAAGVVADDLQDQYIPELLKISALGELNTTVYSGLAGRGTLSIPKAA
                     GVSPVFKFYGEAEAQDDSIASFTKVTLSPKAFGGAIPLSKQAILTAPNIESFVQSELL
                     RYAAQGLEQNVMDKVVAAAPVFNVETAGSITLEDVQGAVAKLAQANVDMRGVKAVMNA
                     KTLSTLRQIAVLDNTAAKAMVEGYRSTEMWLAD"
BASE COUNT         1708 a          936 c         1054 g         1421 t
ORIGIN      
        1 atgataagaa cgccgccaat aactctaaac aaacacaaat ggtgaacggt acttttatct
       61 attggttgaa cctcaacacg ccggggaacc ttcgcggtat cacaacacgc tgtgttctgt
      121 tagatgaggt cagtagctgc gaaatcactg atgaagggaa cccaatcaaa ctggcagaag
      181 cacgtaccag taccttcggt agtgactcac tggtggttgt atcttccacg ccattgtata
      241 aagacgattt aatcaatgct gagtacaacc tcagtgataa acgtaggtac tttgttactc
      301 acacatgcgg tcatgaatat acttttgaat gggaacaagt agcgtttgaa tttaagcaat
      361 tagaaaatgg tagatccatt cctgatagta ctactactcg tttgttatgc cctcattgca
      421 atgagggaat agatgaacat acacggcatc agatggttga taatggtcga tggattgcaa
      481 ctaatccaga tggggaaccg ggtgtagtag ggtatcaaat cagtagaatg tattcacctc
      541 tgaatactat tactgaaatg gtttcaaagt ttgctgatgc tctatataac ttcaatcttc
      601 aaacctttta caataacgaa ttgggcttgc cattcgaaga tgagtatcaa aaagaattag
      661 atattctcca attagaatcc ctacgagaag atgaatttaa cctttataaa attcctgaaa
      721 gtaccttagc gttgtgtatt tcagttgatc agcagggtga taggctggaa agtctgcttt
      781 taggttttga tgaaaagaac atttatgtac ttgggcatga gtttttctac tcccacgatt
      841 gtacgaagat tgaagcccca gcttggaagg atttagatca gttttgccgt caggatttca
      901 gtactgtatc aggtcgcata gtacctacac tggctgtgtt cattgatgca gggaacggta
      961 acgctacgga tactgtgaaa aaattcacta cacgttggag taagtatcat ccgatcaagg
     1021 gtagcagctc cactactggt gaactattca agaccagtac tcaggcgggg tacaaactac
     1081 agatcctaaa cgtacatgaa cagaagaaca cgatacgccg cctcctgaac ctgatgctat
     1141 ccagtgaggc agataacgcc ccggttaagc tccgcttcag tagtacctta ccatcagatt
     1201 tctttgagca actttcagca gaggagttaa aacccgctgg tggtaagttg gtatggcgat
     1261 taaagaaagg tcaaagaaga aatgaggctt tagacctaat ctgttatgga atgatcgcca
     1321 tagcatatgc ccaatcaaaa ttaggtactc aaccattcag gaagttacgc gattataaag
     1381 cacaggaaac cactaaatac gagataaata aagtagaaga accaaaacca gaaacagtac
     1441 aaaaaccaaa acggaataga cgtactggaa tgggatcaaa ctggttcggt aaaagataag
     1501 gatatcaaaa atgacacttc taccagaaaa gatctacatg gtatcgaatc ccatggatct
     1561 aacggtatta gtacccgctg ctactattct tgttattagt tttacatcat ctggaaaaag
     1621 tacctcatta gataatttaa atggtagtac ttcaaaagaa tttacggttg tattagatat
     1681 gagtacagca caggaattac tattctgtac tcaaatatct aatggtcaaa ccagtacttt
     1741 tacttctgaa gtcattaatc cattacttta tacttctgag tactcccaat taaaacagat
     1801 gatcagtgaa attgatcagg ttatagctaa taaaattgca ggcggtgcta attactcaat
     1861 aacaattaat aataagactc ttgtaagtga atcattatca tcgcttgaat caatgcggga
     1921 acgctatgta aaacgtgcta atgctctatt tgtgaaaatg aatggcggta ctttctctaa
     1981 tggcggtaaa ccaatcaaga gtattacggt tttcaggcct aagccaggga gtacacgcta
     2041 atgttctgga ataagaaaaa taaaaattca gaaactaaac ccaagaagat taaacaggaa
     2101 cgttccttaa aacaaagtac cttaaaacga gatctacagg cagtacgcaa cacatcagta
     2161 atgaacttcg gattcaattc aaactcaggc agcaatatca acttccttct gatgaaagca
     2221 ctacctacct tcagggcttt ctcacgtgat gcagtactta agaatcctat tggtcgtaag
     2281 tacatgaacc tatcggtaga tggcgttgta ggctctgatg gcctgtacgt taaaccttca
     2341 gtagagattg acggtactga agaagaaatc aacggaatca atctaacctt ggaaaagctc
     2401 tttgaccgtt gggcatatga tgctgatcgt tttagtgttg atggctcatt aagttttgac
     2461 ctgtttcagc agaacgcaga gaaggtaagg gtccaggatg gtgaatgctt tattcgtata
     2521 cacaatatta ataataccat caagttagaa attctcgata cggttcgttt gcttcagtta
     2581 aataatcaac gtttgagtaa tggtaattat attagtaatg gtattgaatt tgataaatgg
     2641 cataagccag ttaattacta tttctgtaaa ttcgatcctg ttacctatac ttatatcact
     2701 ggcgattatg aagtaattcc cgctgcggaa atctgtcact actttattgc tgatcaacaa
     2761 ggccaggaac gcggattgcc ggacatggtt gcaacttcca aattaatgga agatctcaag
     2821 aacttcactg aggctgcgtt aactgcgaaa cgtgtatctg catcgtcaat ggcttttatt
     2881 accaataata acgacactac cagtactgat ctgttagggg ctgatgaacg agaagaagtt
     2941 acaccagtat atacagagta ttttgaagct ggctttattg gtgagttagc cgaagggcag
     3001 gatattaaaa ctgttacacc tactaatggt gttgatggga ttgatcaatt taccaatgaa
     3061 ttgatgaatc agatctcgat gggtctgaac ataacaaagc aagccttatt atctgataca
     3121 tccaatgctt catttagtgc aagccgactt actgagaaat tacaacaaac tacattccgt
     3181 acccgtacta atgtactaat cagtaaagta cttaaaccaa tttatatcgc ctggttaaag
     3241 aacgaaatgc taaataacaa taagttaaat ctaagtttct tgagctttga tgatcttatt
     3301 tgtgctcggt atatttctca aaagccaatt agtttagatc ctgtaaagga tatacaggct
     3361 gaactactcc agttagaagc aggtattaaa tctaaaacac aggttattgc tgagttaggc
     3421 ggtgatccag tgaaggtatt agcagaggta caggcagaaa aggataaaga aaataaaaac
     3481 aaggaagtaa tccaggatgg aaatgaaaaa ccagaagagg gaactaacga aacccctaca
     3541 ggcgattaat acagaatcgc gtaccatcga agtagcgttt tgttcagaaa cccctgtaga
     3601 acgtgaaatc aacggtcaaa tctataacga aattcttcta tgtggttcca gtaacgcaga
     3661 cctaacccgc cttaataaca atggggcagt actctttaac cataaccgtg atgatcttat
     3721 tggtgctgta gttgatgcac gagtggatgc tgatcgcgtt ggtagagcta cattacgaat
     3781 tagcagtacc gccaacgatg aatgggaaat gattcaggaa ggcgtactaa cccatattag
     3841 cattggctat aacattaatg attatcaaat tgatggtaat aatatctatg ttaccaacta
     3901 cgaaatttat gaaatctcat tagtaacagt acctgccgat cctaccgttg gggtaggtcg
     3961 ttcaatggac tcacaacttg cattgaaatc actaaataaa gaaggtgaaa caattcaaga
     4021 gggtcaaatg gatatggata atccagaaaa cgaaaaggaa tcaattgttg atagtactga
     4081 agaagtaaat gaaacaattg aagaggcatt agtagagcca tcggtcgaat atgcagaaga
     4141 aatcaaacaa gaattaagtg atgaagaact tttagaaatt atctctactc gacctgattt
     4201 gctcaataaa gtacatggtg aagaaccaga aagtataaat agtacagaag atactgaacg
     4261 tgttcgtgaa ttagaagcac tcggtaaagt acttaatatt gatgtatctg aagcaattga
     4321 aacaggaatt tcaatttcag atttcaaacg tcaattgaat gacattaaac aaaatcctct
     4381 tcatgataag gaaatcaaag aaatggataa taaaaatcta ttaaaagata tggtacgtgc
     4441 cattaaaact ggcgataaat ctgctcttga agcatatgaa cgtggtatta atggttttgt
     4501 acgtgcggct gtaccaagta ctaatactac taccgctgct ggcgttgtag ctgatgacct
     4561 acaagatcaa tacattccag aactactaaa aatttctgca cttggtgaac taaacaccac
     4621 tgtttattct ggccttgcag gtcgcggtac tctaagtatt ccaaaagcgg ctggtgtatc
     4681 tccggtattt aaattctatg gtgaagcaga agcacaggat gattctattg caagctttac
     4741 taaagtaacc ctaagcccta aagcttttgg tggtgctatt ccactatcca aacaagcaat
     4801 tctaacggct ccaaatattg aatcatttgt acaaagtgaa ctactacggt atgccgctca
     4861 gggtcttgag caaaatgtaa tggataaagt agttgccgct gctccggtgt tcaatgttga
     4921 aactgctggt tctattactc ttgaagatgt tcagggtgca gtagcgaagc tggcacaggc
     4981 taacgttgat atgcgtggcg ttaaagctgt tatgaacgct aaaaccctaa gtaccctacg
     5041 tcagattgca gtacttgata acactgctgc taaagcaatg gttgaaggtt atcgcagtac
     5101 tgaaatgtgg cttgctgat
//
LOCUS       DAZPEP010000225         4992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZPEP010000225 DAZPEP010000000
VERSION     DAZPEP010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.227"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            12..754
                     /locus_tag="ACHUT9_004056"
                     /pseudo
     CDS             12..754
                     /locus_tag="ACHUT9_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="ACHUT9_004057"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="ACHUT9_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            1312..1458
                     /locus_tag="ACHUT9_004058"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="ACHUT9_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUT9_004059"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="ACHUT9_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HFR1350406.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="ACHUT9_004060"
     tRNA            complement(2777..2851)
                     /locus_tag="ACHUT9_004060"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="ACHUT9_004061"
     CDS             complement(2927..3859)
                     /locus_tag="ACHUT9_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFR1350407.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUT9_004062"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="ACHUT9_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFR1350408.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1212 a         1160 c         1167 g         1453 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 catcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgcttgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc ga
//
LOCUS       DAZPEP010000226         4930 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZPEP010000226 DAZPEP010000000
VERSION     DAZPEP010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4930
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.228"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..541
                     /locus_tag="ACHUT9_004063"
     CDS             <1..541
                     /locus_tag="ACHUT9_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1350409.1"
                     /translation="KKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDE
                     DLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQT
                     DLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPG
                     KDATPGNVYAQADYKIAYE"
     gene            601..3105
                     /locus_tag="ACHUT9_004064"
     CDS             601..3105
                     /locus_tag="ACHUT9_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1350410.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYMNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSASLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3121..3870
                     /locus_tag="ACHUT9_004065"
     CDS             3121..3870
                     /locus_tag="ACHUT9_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1350411.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3872..>4930
                     /locus_tag="ACHUT9_004066"
     CDS             3872..>4930
                     /locus_tag="ACHUT9_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350412.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITY"
BASE COUNT         1528 a         1010 c         1017 g         1375 t
ORIGIN      
        1 taaaaagtca cttcttgttg ctggcgtagc aatgattctt tccaataacg tatttgctga
       61 cgaaggtcac ggcattgtta aatttaaagg tgaagttatc tctgctccat gttcaattaa
      121 accaggtgac gaagacttaa ctgtaaatct gggtgaagta gcagataccg ttctgaaaag
      181 cgaccagaaa tctttggctg agcctttcac tattcacctg caagactgca tgttgagcca
      241 gggtggaacc acttattcaa aagcgaaggt tactttcacc acagcgaata ctatgacggg
      301 tcagactgac ctgctgaaaa acaccaaaga aactgaaatc ggtggtgcca caggcgttgg
      361 cgtacgtatt ctggatagcc agagcggcga agtgacttta ggtactccgg tagtaattac
      421 attcaacaat actaacagct atcaggaact gaacttcaaa gctcgtatgg aatccccggg
      481 caaagatgct accccgggca acgtttacgc tcaggctgac tataagattg cttacgagta
      541 atcacattag gccgggttcg cccggccaca tcattaacgg attaatgata agtggaacag
      601 atgtataaaa aattaaaatt aaccacaata agcgaattga ttaaaaatat ttattgttca
      661 ttatccgttc tcatcattgg ttgtgcgtca gcttatgccg ttgaattcaa caaagattta
      721 atcgaagccg aagatcgtga aaacgttaac ctttcccaat ttgaaactga tggccaatta
      781 cccgtaggta aatactcatt aaacgctctt attaataata aaaggacgcc aatccatctg
      841 gacctgcaat gggtattaat tgataaccaa actgcagttt gtctgactcc agagcaatta
      901 acattattag gctttactga tgagattatt gaagaggctc agcaaaacct gatcgatggt
      961 tgttacccta tcgaaaaaga aaaacaaatt acaacttatc tcgataaagg gaaaatgcaa
     1021 ttatccatat ctgcacctca ggcatggtta aaatacaaag atgcaaactg gacgcctcct
     1081 gaactttggg atcatggtat tgctggggca tttcttgact acaatttata tgcctctcat
     1141 tatgcaccac atcagggcga taattcacaa aatataagtt cctatgggca ggctggggtt
     1201 aatcttgggg cctggcgcct gcgtactgat taccagtacg atcagtcatt taacaatggc
     1261 aaaagccagg cgaacaacct ggattttccg cgtatttatt tgtttcgccc aatcccagca
     1321 attaatgcaa aactaactat aggtcaatac gatactgaat cctctatttt cgactctttc
     1381 catttttctg gcgtttcgtt gaaaagcgat gagaatatgt tgccgcctga tctacgtggt
     1441 tacgcaccgc aaatcacggg tgttgcacaa acgaatgcaa aggtcactgt ctcacagaac
     1501 aaccgtatta tttatcaaga aaatgttcct cctggcccat ttgctattac taatttattc
     1561 aatacattac aggggcaact ggacgtcaag gttgaagaag aggacgggca agtcacgcaa
     1621 tggcaagttg catctaatag tattccttat ctgacgcgta aagggcagat tcgctacacc
     1681 actgctatgg gtaaaccgac cagcgttggt ggtgattcct tacaacaacc cttcttctgg
     1741 actggtgaat tctcatgggg ttggctgaac aatgtatccc tgtatggtgg ttcagtttta
     1801 acaaaccgtg attatcaatc tctggctgcc ggcgttggct ttaaccttaa ctcattgggt
     1861 tcattatctt ttgatgtcac acgatctgat gctcagttgc ataatcagga taaagaaacg
     1921 ggttatagct accgcgctaa ctattcaaaa cgttttgaat ctaccggtag ccagctcact
     1981 ttcgctggtt accgtttctc tgataaaaac tttgtgacaa tgaatgaata tatcaatgac
     2041 actaaccatt acatgaatta tcagaatgaa aaagagagtt atattgtcac gtttaaccag
     2101 tatcttgaat cattaaggtt aaatacatac gtaagtttgg ctcgtaatac ttactgggac
     2161 gccagcagta atgtgaatta ttcattatca cttagccgcg attttgatat cgggccatta
     2221 aaaaacgtct ccgcttctct aacatttagc cgaataaact gggaagaaga caaccaggat
     2281 caactgtacc taaatatttc gattccctgg ggaaccagta gaacattgag ctatggtatg
     2341 caacgaaatc aggataacaa gatttcgcat actgcttcgt ggtatgactc ttccgatcga
     2401 aataattcct ggagcgtttc tgcttcaggc gacaatgatg aattcaaaga tatgaaagcg
     2461 tcactacgcg ccagttatca gcataatacc gagaacggtc gtctctatct ctccgggaca
     2521 tcacagcgag acagttatta ttctctgaat gccagttgga atggttcatt cacggcgact
     2581 cgtcacggag ccgctttcca tgactatagt ggtagtgctg actcgcgttt tatgatcgac
     2641 gcagatggtg cagaagatat tccgttgaac aataaacgcg cggtaactaa tcggtatggc
     2701 atcggagtta ttccatcagt cagcagttac ataacaacat cattaagcgt tgatacccga
     2761 aatcttccag aaaatgtgga tatcgaaaac tcggttatca ccaccacctt aaccgaaggt
     2821 gctattggct acgccaaact tgatacccgc aagggctatc aaatcatggg ggttattcgc
     2881 ctggcagatg gtagtcaccc accactgggg attagcgtaa aagataaaac cagccataaa
     2941 gaattaggac tggttgctga tggcggcttt gtatacctca acggcattca ggatgacagc
     3001 aaacttactt tacgctgggg tgataaatcc tgttttattc aaccacccaa tagcagcaac
     3061 ttaaccaccg gaacggttat tttaccgtgt attagccaaa attaattcag gatgaaacta
     3121 atgcgctact taaacacaaa gaatctcatt gcagcaggag tattactcgc ctgcatgagc
     3181 agcattgcct ggggtgccat catcccggac cgtacccgta tcataatgaa tgagtcagat
     3241 aaaggggaag cattaaaact tactaatcaa agcaaaaatc taccctatct ggcgcaaaca
     3301 tggatagaag atacgaaagg caataaatca cgtgacttta ttgtcacggt tccccctatg
     3361 gtacgtttaa atcccagtga gcaaattcaa ataagaatga taagtcagga aaaaattgct
     3421 caactgccta atgacagaga aacgctcttc tattttaacg tgcgagaaat accgccaaaa
     3481 acagataaaa aaaatgtgat gcaagtcact atgcagcacg cattgaaact attctggcga
     3541 ccaaaagcta ttgaattaga agatgatggg gtaatgtctt acgaaaaagt tgaaatcatc
     3601 cgtagaaatg atggttcgat ccgctttaac aataagatgc cttatcacgt tacgctgggt
     3661 tatatcggaa caaacggaat cacaatgttg ccacaaacgc aaagtttaat ggtaacccct
     3721 tatagccatg cagatacgca gtttaaaaat gttccgtcag cttttcaggt tggttatatc
     3781 aatgattttg gtggattaag tttttatgaa ataaactgcc cgacggttaa taactcttgt
     3841 aatgtttccg tagccaaacg agataaataa tatgcgctac ttattaattg taattatatt
     3901 tattataggt tttagtgcat tacctgtatg ggcaatggac tgctatgctg aacatgaagg
     3961 gggaaatacc gtagtcattg gttacgtacc aagaatttct atccccagta atggtaaaaa
     4021 aggtgataag atctggcaaa gcagtgagta ttttatgaat gtcttctgta ataatgcatt
     4081 acctgcccca tcacctggag aagaataccc atcagcctgg actaatataa tgatgttttt
     4141 accaggcggt caggactttt ataatcagaa ctcctatatt tttggcgtaa catacaatgg
     4201 agttgattat gattcgactg cccctaatgc tatcgcagca ccagaatgca ttgatataaa
     4261 aggagcgggt acctttaata atcactataa aaaccccgct gtctgtagtg gaggtcctga
     4321 gcctcaatta tcagtaacct ttccagctcg cgtacaactc tatattaaat tagcaaaaaa
     4381 tgccaataga gttaataaaa acctggtgtt acctgatgaa tatatcgctc tagagtttaa
     4441 aggtatgagt ggcgcaggag ctatagaagt agacaaaaat ttgaccttca ggattcgtgg
     4501 attaaataat attcatgtcc ttgactgctt tgttaatgtc gcactggaac cagctgatgg
     4561 cgttgttgac tttggtaaaa taaattcccg aacaattaaa aataccagcg ttagtgagac
     4621 gtttagtgta gtcatgacaa aagatcctgg tgcggcctgt actgagcaat tcaatatttt
     4681 agggagcttt ttcactacgg atattttgag tgattatagc catctggata tgggtaatgg
     4741 actgctattg aagatatttc ataaagatgg aacagcaacg gaatttaacc gcttctcaca
     4801 atttgcttct ttttcatcgt ctagtgcacc ttcggtcacc gcaccattca gggcagaact
     4861 tagtgcgaac ccggcagaga ctgttgttga gggaccgttt agtaaagacg taatcctcaa
     4921 aatcacctat
//
LOCUS       DAZPEP010000227         4844 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZPEP010000227 DAZPEP010000000
VERSION     DAZPEP010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.229"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(34..954)
                     /locus_tag="ACHUT9_004067"
     CDS             complement(34..954)
                     /locus_tag="ACHUT9_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1350413.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHUT9_004068"
     CDS             1092..1991
                     /gene="pgrR"
                     /locus_tag="ACHUT9_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFR1350414.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2328..3941
                     /gene="mppA"
                     /locus_tag="ACHUT9_004069"
     CDS             2328..3941
                     /gene="mppA"
                     /locus_tag="ACHUT9_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFR1350415.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHUT9_004070"
     CDS             complement(3992..>4844)
                     /gene="ynaI"
                     /locus_tag="ACHUT9_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFR1350416.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1307 a         1081 c         1140 g         1316 t
ORIGIN      
        1 ccatatcact attgcggatt tgaatatgtt tcattacaga gtttcctcaa agaacggagc
       61 taatactgaa attgcttcag ccacataggc tttgccgtca taaagatcca tatggtttgc
      121 gccttcaaca atgtgatagc gtttatcttg gcttgatgct cgatcgtaca ggtcgtcact
      181 catccatttg ctccctgcct ggctgcccgc cacaatctgc atcggctgag tcaggtacac
      241 ttccgccata tggtaagcat caaaggtaat aatctggtta aggctgcgca aagtagcgta
      301 acccggtgct gttggatact gcgcgcgagg ggtgtggtaa tactcccagg cctgacgcag
      361 ttcttcgttc ggcgcatcgg actccttcat tggtgccagt ggcataacgg catattctcc
      421 gctgctaata tcgctggttc gggcgtttga acccgcttca acgtatggca gggcatcaat
      481 agatttcaca ttgttttccc aaccattacg gaacatcgaa ccaatattga ccgcactaac
      541 ggtaccgatg gccttgatgc ggcgatcctg aattgcagca ttggctgtat atcctgcacc
      601 ggcacaaatt cccatcgcac caattcgggt attgtcgaca taagaaagcg ttgtcaggta
      661 atcaatcacg gcactgatgt cttcagtacg aatgtatggg ttttctaact gacgcggctc
      721 gccgccactt tcaccttgat aagatgcgtc ataagcaata gtgacaaatc ccttttccgc
      781 cagttttttg gcataggttc cggccgtttg ttctttaacg cccccacctg ggtgagatac
      841 cacaattgcc tgatactgac gggtttcatc aaattttgga gggaaataga tcactgcaga
      901 caaagagatg gttggattat tgctgttagt gaagctgact ttattattca tcattccgtt
      961 cctcatggag ttgtcggttc gttttaacgg ttggtgatat cactatagat attgatcatt
     1021 aagttgatta gaccctaaat tatgattaga cctatcattt aaatgattaa tagattgcat
     1081 ggagatactg aatgaagcgt gaagaaatcg ctgatctgat ggcgtttgtc gtcgttgcag
     1141 aggagcgtag cttcactcgt gcagcagccc gcctgagcat ggcgcagtca gctttaagcc
     1201 agatagtgcg tcgtatagaa gaacgattgg gattgcggct tctgacgcga accacgcgca
     1261 gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt tcttggccct atgttgcatg
     1321 acatagattc agccctggca tccctgagcg agctgcagaa ccgcccatcc gggacaatac
     1381 gtattactac tgtagaacat gcagccaaaa cgatattgtt accagcaatg cgcacattcc
     1441 tgaaatcgca tcctgaaatt gatattcagc tcaccattga ttatggtttg accgatgtcg
     1501 tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga gatggataaa gatatgatcg
     1561 ccattcgaat cgggccagat ataccaatgg ctattgttgg ctcaccggat tatttttctc
     1621 gccgaagtgt tccaacgtca gtgtcacaat taatagatca tcaggcaatt aatttgtatc
     1681 ttcccacatc gggtacagca aatcgctgga gattaatacg cggtggacgt gaagttcgtg
     1741 ttcgcatgga aggtcagctt ttactgaata cgatagacct gatcattgat gctgcaattg
     1801 atgggcatgg attggcgtat ctaccttatg atcaggttga gcgggctatt aaagaaaaaa
     1861 aactgatacg tgttttggat aaattcacac cagatttacc cggttatcac ctgtactatc
     1921 cacaccgtcg acatgctggc tcggcattct cattatttat agataggctg aagtataaag
     1981 gtgctgttta gcactacttg ctgatacatt aatttaattt ttctcttaac gtattctcag
     2041 ttcctttcaa cgttttggtt attttttatt cttcgtacaa tggcgacaga tgctgattat
     2101 gataccgaaa actggtttga acgtgcgaag cctgaacgta gtgttcggtg ttctatgtgc
     2161 tttaccgcat tttgtcgtct attatttaca ctaaatctga tttaatatat tgatacataa
     2221 aacattttat gctccctgtg tcactttttt gatggaagtt gtttgcattt ctttaaggcg
     2281 aaacaaataa ttacgcatca attttaatgt cggttagagg gaaacttatg aagcactctg
     2341 tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc tctttcgtat gctgcagaag
     2401 ttccgagcgg cacagtactg gcagagaagc aggagctggt gcgccacatt aaagatgagc
     2461 ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga gattcaggtc attcgcgatc
     2521 tgtttgaagg actggtgaat cagaacgaga aaggggagat tgtccccggc gttgcgactc
     2581 agtggaaaag taatgacaac cgtatctgga cttttaccct gcgcgataac gcaaaatggg
     2641 cggatggcac accggtaacg gcgcaagatt ttgtctacag ctggcagcgt ctggtggacc
     2701 cgaaaacgct atcgccattt gcatggtttg ccgcgctggc gggaatcaac aacgcacagg
     2761 cgattattga tggtaaagct acgccagacc agcttggcgt caccgcagtt gatgcccata
     2821 ctttgaaaat tcagcttgat aaaccgttgc cgtggtttgt gaatttaacc gctaactttg
     2881 ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa agagtggacg aaacccggaa
     2941 atctgatcgg caatggcgct tatgttctta aagatcgcgt agtcaatgaa aaactggtcg
     3001 tggtaccgaa tacccattat tgggataacg ccaaaacggt actgcaaaaa gtgaccttcc
     3061 tgccaattaa tcaggaatcc gcagccacta agcgttacct cgcgggggat attgatatca
     3121 ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa ggatattccg gggcaggttt
     3181 atacgccgcc gcagctcggg acctattatt atgcgtttaa cacgcaaaaa gggccgacgg
     3241 cagatcagcg cgttcgtctg gcattaagta tgacgataga tcgccgcctg atgaccgaaa
     3301 aagtattagg gacgggcgaa aagccagcgt ggcattttac accagatgtt accgccggat
     3361 ttacgccgga accttcgccg tttgaacaaa tgagtcagga agaactgaat gcgcaggcaa
     3421 aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc gctgaagctg acgcttttgt
     3481 ataacacttc agaaaaccat caaaaaattg cgattgctgt agcatcgatg tggaaaaaga
     3541 accttggcgt agatgttaaa ttgcaaaatc aggaatggaa aacctatatc gatagccgta
     3601 acaccggcaa ttttgatgtt atccgcgcct catgggtggg ggattataat gaaccctcca
     3661 ctttcctgac attattaacg tcaacgcatt caggaaatat ttcacgcttt aacaatccgg
     3721 catacgacaa agttctggcc caggcatcga cggaaaatac cgttaaagcg cgtaatgccg
     3781 attacaacgc ggcagaaaaa atcctcatgg agcaagcacc gattgcacca atttatcaat
     3841 ataccaatgg acgattaatc aagccgtggc tgaaaggtta tcccattaat aacccggaag
     3901 atgtggcgta cagccggact atgtatattg tgaagcattg atgtgatggg aactggcgtt
     3961 acccttgtgg ataacgccag tgatgtctga tttagcgccc ctgttccggc ggtgtaatgt
     4021 tatccatata cagcgtctgg ctggggaagg caaagtccgc gccgtgtgac tgcacaatat
     4081 cgataatctt caaataaacg tcttgctgtg cagcaagcca ttcagcccat accgtggttt
     4141 tggtaaagca ataaaccata atattcaatg acgagtcggc aaactggttg aaataaacca
     4201 gtaaggtttg tcgctggtcg atggccgggt gatttttcag catctcacgt acggcttcga
     4261 caataacgcc cacttttgcc gcatcctcat aacgtaaacc aatggtcgtg gtaatgcggc
     4321 ggttggtcat tcgtcctggg ttttctacgc tgatcgacga aaacagcgag ttcggtacgt
     4381 acaatggacg attatcaaag gtcgtaattt tggtaattcg ccagccaatt tccgctactg
     4441 taccttcgat atttctgtcc ggtgaacgga tccagtcgcc aatactgaaa ggacggtcga
     4501 aatagagcat gatcccggaa aagaaattac tcagaatatc tttaccggcc ataccgacag
     4561 ccagaccacc aataccacca aaggtcagca agccagaaag gctcatgccg aaatgttcgc
     4621 cataaagcag aacaagcacc acaataatgg tgattttgat aatgcgcgac ataatccgcg
     4681 cactggtgat atcgcgacct tttttaatct gctgtttttc aaactgatta atcagcaaaa
     4741 atagcttaat cgtcagaata accgcaatca gggacgtaca gataaaatcg ataacgtctg
     4801 gggtgataaa tttgagttta tagttttcta taacataatt aata
//
LOCUS       DAZPEP010000228         4840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZPEP010000228 DAZPEP010000000
VERSION     DAZPEP010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.230"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            488..1231
                     /locus_tag="ACHUT9_004071"
     CDS             488..1231
                     /locus_tag="ACHUT9_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFR1350417.1"
                     /translation="MNRKQFIQSHGATCSNWTWSWSFVNHDKKMVIFGAWDVQKEQER
                     SVILRERWKTKVVEGKERKNLGYIQAIEHIQLIAEGYELYTFDMEQGRNDENRDVAVI
                     KRFTPKLEKRYLRKEGTVWYADFLPNPFPDEITSPENYAEGAKKQVTVNSYERDPKAR
                     QACIDHHGTSCGCCEFNFEKVYGEHAKGFIHVHHIKPLHTVGEDYEVNPITDMVPLCP
                     NCHAMVHRGNEVLSVESLKALLFKDSNKI"
     gene            1314..1787
                     /locus_tag="ACHUT9_004072"
     CDS             1314..1787
                     /locus_tag="ACHUT9_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006320261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350418.1"
                     /translation="MSITTIAATYKDVLTIAFSALSFIIACTSLYFSGRAAWHDRSRL
                     KITADLVYESFDEEPYKIEVIVINVGRRDAVLEGMLCHYEKGIRSHSSPKESIVIKEK
                     QRKKFELVSSDLVTIDNECDICHLEDITILDVEGNEHKIPNSHRLIALLKQDKKT"
     gene            complement(2044..2712)
                     /locus_tag="ACHUT9_004073"
     CDS             complement(2044..2712)
                     /locus_tag="ACHUT9_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350419.1"
                     /translation="MDCRKLNIVLLSTVAVLLVVVILVLIKSLFVGSEGFEWGSVSDW
                     VSSIANLIMAGAALYAAWNAKNWFQTKIKENALNQVTKFWTNCDLFSLEMLKTYENIC
                     NLNNTEPDIEPKNFAHLVKTEREVIDLLNIKLASLKSELQSLTFWKVSPKDEAIFNNY
                     FFSNQIALGTLREAISFDINDIHDRYHYNVSYGKLLVDTFDSLIEAHATLKKDMNNLF
                     VFSN"
     gene            complement(2845..3156)
                     /locus_tag="ACHUT9_004074"
     CDS             complement(2845..3156)
                     /locus_tag="ACHUT9_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012125135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350420.1"
                     /translation="MSKDGYDPSITRGVQKFSHNGRWATVCYWGSALTEFHYGDRVFF
                     QNQYREYWLGTIERDCFVLTCETPLISVIDGLAYLSAEYRMNQSSEEGDWFCDQGELP
                     F"
     gene            3349..3900
                     /locus_tag="ACHUT9_004075"
     CDS             3349..3900
                     /locus_tag="ACHUT9_004075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350421.1"
                     /translation="MNIDNWAIPITSLGGQLLGVELVSHVEREGIRQIGLWEEEVLHG
                     QLMLIESKAAWFRDNGLYCVITTGRGEPYEYLPFLRQMTRDMKVHNQQWLDDLGVHGG
                     SAIPLVTGDFEVARLNRRYTEENIDRLIFPVLIKSIRQYCEKVIVPVRDKTYYPILRE
                     AGVWAVQGEFRPMRFEQCEKLIG"
     gene            4651..>4840
                     /locus_tag="ACHUT9_004076"
     CDS             4651..>4840
                     /locus_tag="ACHUT9_004076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350422.1"
                     /translation="MKITVDNISEMFAGTKELLIGGLITGIVGVLILTRVSGYEYVKV
                     PLMLVMIFLLTVVPGFIAM"
BASE COUNT         1523 a          870 c         1020 g         1427 t
ORIGIN      
        1 gagtacttaa agggcttgcg ttaattgtaa cttattacgg catattacgg catggatgta
       61 tgccgtaata cggcaaaaaa agcccctgaa acccgctatt taccatcaat acggcaacta
      121 agtatagaga tacgaaatcc attccatttc attccatgta tttctttctc tatcagtatt
      181 ccaagaaact gtgacttgcg tcacgaatac tactcgtcgt gaactcctcg tagtattggt
      241 tagtaacgtt gcgtttttct cgccagtact cgaaaacccg caaggcgtta ctactcgctt
      301 tgctcgatag atgttagtac agcaaatgaa gtacctaaag tgaattggta agtacgacat
      361 gccccgccag ttccacaact ctcgctctgg tcatttccac tactatgatg ctattggata
      421 gctacataaa gtactggatc aagtaggcat tttttgtgta caaagaggga attaagagag
      481 ggataccatg aacagaaaac aattcatcca gtcgcatggt gctacttgta gtaactggac
      541 ttggagttgg tcatttgtga accacgacaa gaaaatggtt atctttggtg catgggatgt
      601 acaaaaagag caagaaagat cagtcattct gagggagcgg tggaaaacca aagttgtgga
      661 gggaaaggaa agaaaaaatc ttggatatat tcaagctatt gaacacattc aattgatagc
      721 tgaaggttat gagctttaca cgtttgatat ggagcaggga cgaaacgatg agaatagaga
      781 tgtagctgtt atcaaacggt tcactccaaa acttgaaaaa cgttaccttc ggaaagaagg
      841 aacagtttgg tatgcagact ttttacctaa tccatttcca gatgaaatta cctcacctga
      901 aaactacgct gagggtgcaa aaaagcaagt tacagtaaac tcctacgagc gtgatccaaa
      961 agcacgacag gcttgtattg accatcatgg tacttcctgc gggtgttgtg agtttaactt
     1021 tgagaaagtc tatggtgaac acgccaaggg ctttatacac gttcatcata tcaaaccgct
     1081 acacacagta ggcgaggatt acgaggtaaa tccaattact gatatggttc cactttgtcc
     1141 aaactgtcac gcaatggtac atcgcggtaa cgaagtatta agtgtcgaaa gtttaaaagc
     1201 actgttgttt aaagacagca ataaaatttg aatttatttt agacttgata cgacatactt
     1261 gaaaagttat gaatacaata tgctttaaat tagacatgta agttgagaga aaaatgagca
     1321 taacaacaat agcagctaca tataaagatg tgttaactat tgctttttcg gcgttgtcat
     1381 ttattattgc atgtacttcc ttatacttct ccggtagggc agcatggcat gatagatcaa
     1441 ggctaaaaat aacagctgac ctcgtctacg agtcttttga tgaggaacct tataaaatcg
     1501 aggtgatagt gatcaatgtt ggtaggcgtg atgcagtttt ggaagggatg ctttgccatt
     1561 atgaaaaagg cattaggagc cattcatctc caaaagagag cattgttatt aaagaaaagc
     1621 aaagaaaaaa atttgaattg gtaagctcag acttagtcac gatagataat gaatgtgata
     1681 tttgtcattt agaagatata actattttag atgtggaggg gaatgaacat aaaatcccta
     1741 actctcacag actcattgca ttacttaagc aagataaaaa gacttaagaa taatattttt
     1801 tggaatgtaa ggtaaatgtt agatgccact atagattgag ggttagaact actaacccac
     1861 tatagggttt acgagatgat aagaatggaa tttaacacct acgatggtga tatatattac
     1921 ttcctctatt tatataggag gtacgaaagg gggagtactt taatgaagac cttataaatc
     1981 ttaatgttga gatgttagtt atagcggtga ggatacccca ccgtctattg ggagttgtgg
     2041 actctaatta gagaaaacga ataagttatt catgtccttt ttcaaggttg catgtgcttc
     2101 tattaaacta tcaaatgtat ctactaataa ctttccatag ctgacattgt aatgatatct
     2161 atcatgaatg tcatttatat caaaacttat cgcttccctc aatgttccta aagcaatttg
     2221 attactgaag aaataattat tgaatatagc ttcatctttt ggcgatactt tccaaaaggt
     2281 aagtgattgc aattcgcttt ttaatgaggc taacttaatg tttaatagat cgataacttc
     2341 tcgttcagtt ttaactaaat gtgcgaaatt ttttggttct atatctggtt cagtattgtt
     2401 aaggttgcat atattttcat atgttttcaa catctccaac gaaaaaagat cgcaattagt
     2461 ccagaactta gtaacttgat ttaaagcatt ttcctttatt ttagtttgaa accaattttt
     2521 agcgttccat gctgcatata gggctgctcc agccataatg agattggcaa tagaactaac
     2581 ccagtcacta accgatcccc actcgaaacc ttcagaacca acaaagagtg attttattag
     2641 tactaaaatt acaacaacca gtaatactgc tacggtcgat aaaagaacga tatttaattt
     2701 tctacaatcc atcgaaagta gttcctgaac gcattaaagg ggcaaatact ttaccatccg
     2761 aaacacaatc aagtacacta cccgttcacg tactgctcac atgttgggtt tgttattttg
     2821 ttggggtgag cagtacgtgt atcctcagaa gggtagttcc ccctgatcac agaaccaatc
     2881 accttcttca ctgctttgat tcatccggta ctcagcagat agatatgcta acccatcaat
     2941 aacacttatt agtggtgttt cacaggtaag tacaaaacaa tctctctcta tagtacctaa
     3001 ccagtattcc ctgtactgat tctggaagaa taccctatcc ccataatgaa attctgttag
     3061 tgcagatccc caataacaca cggttgccca gcgaccgtta tgtgagaact tctgaacccc
     3121 tcgcgtaatg gatggatcat agccgtcttt gctcatttgt taccctcctc tatgtattac
     3181 tgtatatatg tacagtattg cagtcagttt cccaaaaatc cagcacccct ttgtttacca
     3241 ctttgaatga ttaattctat tgtaatgatc aataaaattg atcgatttaa ttgccaatgt
     3301 tttaaaaatg atcaattatc aataacttaa agcgtaacga gcaaagttat gaacattgat
     3361 aattgggcaa taccgataac aagtttgggt ggtcagttgc tgggggttga gttggtcagc
     3421 catgtagaac gcgagggaat caggcagatc ggcctatggg aagaagaagt acttcatggg
     3481 caactaatgc ttattgaaag caaggcggca tggttcaggg ataacggttt gtattgtgtg
     3541 attactactg gccgtggtga accatacgag tacttaccat tccttcggca gatgacaagg
     3601 gatatgaaag tacataacca gcagtggctt gatgatctcg gtgtacatgg tggttctgct
     3661 attcctctgg ttacaggtga tttcgaggtt gcccgattaa atagaaggta cacagaagag
     3721 aacattgacc gtctgatctt tccagtactc ataaaaagca ttcgccagta ctgcgagaag
     3781 gtgatcgtac ccgtcagaga taaaacgtat tacccaattc tcagagaggc cggtgtatgg
     3841 gcggtacagg gagagttcag gccaatgcgt tttgagcaat gtgaaaaatt aataggataa
     3901 caataatgaa aaaattacta attgggttac tgattgccct tagtactgga tgtactacta
     3961 cgcacagctt catggataaa gcttacgttg gctatgtaga tgacaaggcc gtagtactgt
     4021 ttgatgcagg aacacgtaat ggggtgaagt actaccgtcc tcagttccag agtgattgcg
     4081 gtcgcatcta taacaagatc tatcatgatg acaatacaag tactggatgc ctcatacgag
     4141 attcccgcat tgctgaacta catccctata gagagcatgt aagtaaggta gtaccacatc
     4201 agaccgatag tgttcacgat tggttagaag gggtcaattc accatcccat atcctcgaca
     4261 atctataggg tacgtatggg aaaggtaatg gtgattttgt tagtactggc tggggtactc
     4321 ttcaacattg caggtgagga tgcaccccag ccacagtaca cggacaatga gaaccagaac
     4381 gctatggatt tcgcacagtc actaatcaag ggtaagtacc catgcgatat ggtgactatg
     4441 gatagtactg agaatggtac aggacgtatt gatgtacggt gtgatggtaa gtaccagtat
     4501 gagatcatcc ggcatgatgg acaatggcag gtagtatcga agtaatccga aaagtttagt
     4561 gcttaggctg tatggtgaga atctacaggg ctttttcttt atgcggtgat aagatcggag
     4621 tgaacttttt atgaaggaat agttagtaga atgaaaatta cagtcgataa tatctctgaa
     4681 atgtttgcag gaactaaaga actactaatc ggtggcttaa taacaggtat cgtcggagta
     4741 cttattttaa ctcgtgttag tggttatgaa tacgttaaag tacctttaat gttagtaatg
     4801 atattcttac taactgtagt tcctggtttt attgctatgc
//
LOCUS       DAZPEP010000229         4834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZPEP010000229 DAZPEP010000000
VERSION     DAZPEP010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.231"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            138..1112
                     /gene="hemB"
                     /locus_tag="ACHUT9_004077"
     CDS             138..1112
                     /gene="hemB"
                     /locus_tag="ACHUT9_004077"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFR1350423.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1218..2069)
                     /gene="tauD"
                     /locus_tag="ACHUT9_004078"
     CDS             complement(1218..2069)
                     /gene="tauD"
                     /locus_tag="ACHUT9_004078"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFR1350424.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2066..2893)
                     /gene="tauC"
                     /locus_tag="ACHUT9_004079"
     CDS             complement(2066..2893)
                     /gene="tauC"
                     /locus_tag="ACHUT9_004079"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFR1350425.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2890..3657)
                     /gene="tauB"
                     /locus_tag="ACHUT9_004080"
     CDS             complement(2890..3657)
                     /gene="tauB"
                     /locus_tag="ACHUT9_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFR1350426.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3670..4632)
                     /gene="tauA"
                     /locus_tag="ACHUT9_004081"
     CDS             complement(3670..4632)
                     /gene="tauA"
                     /locus_tag="ACHUT9_004081"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1350427.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1112 a         1403 c         1235 g         1084 t
ORIGIN      
        1 attgctattt ctattacaaa gatgtcctgg tctctctttc attctcttct tgtcaacatc
       61 gcgacaactt tcgtaaaaca ctcctgcccc gcttcaggta tactgtgcac cttaattcca
      121 caaacatcag gcagaccatg acagacttaa tccaacgccc tcgtcgccta cgcaaatctc
      181 ctgcgctgcg cgctatgttt gaagagacaa cacttagcct taacgacctg gtgttgccga
      241 tctttgttga agaagaaatt gacgactaca aagccgttga agccatgcca ggcgtgatgc
      301 gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat cgccaacgcc ggtatccgtt
      361 ccgtaatgac ttttggcatc tctcaccata ccgatgaaac cggcagcgac gcctggcggg
      421 aagatggact ggtggcgcgt atgtcgcgca tctgcaagca gactgtgcca gaaatgatcg
      481 tcatgtcaga cacttgtttc tgtgaataca cttctcacgg tcactgcggt gtgctgtgcg
      541 agcatggcgt cgacaacgac gcgactctgg aaaatttagg caagcaagcc gtggttgcag
      601 ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat ggacggccag gtacaggcga
      661 ttcgtcaggc gctggacgct gcaggcttta aagatacggc gattatgtcg tattcgacca
      721 agttcgcctc ttccttttat ggcccgttcc gtgaagctgc cggaagcgca ttaaaaggcg
      781 accgcaaaag ctatcagatg aacccaatga accgtcgcga ggcgattcgt gaatcactgc
      841 tggatgaagc ccagggcgca gactgcctga tggttaaacc tgctggagcg tacctcgaca
      901 tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg cgcgtatcag gtgagcggtg
      961 agtacgcgat gattaagttc gccgcgctgg cgggtgctat agatgaagag aaagtcgtgc
     1021 tcgaaagctt aggttcaatt aagcgtgcgg gtgcggatct gattttcagc tactttgcgc
     1081 tggatttggc tgagaagaag attctgcgtt aatttttttc ctctcagaaa gggggagtta
     1141 acctgttgaa agtacggtga acgccccctc accctaaccc tctccccaag ggggcgaggg
     1201 aaccgtccgc actcgtatca ccccgcccga taaaacggtt tatccccaag gatcgtcgcc
     1261 cgatgcataa tccgccgctg tggcaggtaa tcggcattgg catagtgctg agtcacgcgg
     1321 ttatcccaaa tcgcaatatc attcggttgc cagcgccaac gcacctgaaa ctccggttta
     1381 gtgatatggg cgaataaaaa gcctaacaag gcttcgctct ctttctcgct gacatcgaca
     1441 attcgcgtag taaagccttc attcacaaac agcgcctgtt taccgctcac cggatgcgtt
     1501 cgcaccaccg gatgtagcaa cggaggattt ttcgcgaccg cctcgcgcca gcgttgatgt
     1561 tcctcctcgg ttttgcggta tttgtattcc gggaacgatt tacggaaatc atgctccgca
     1621 cgcagcccgc tcagcagctg gcggaaggga acagagagcg cctcataggc cgcaataccg
     1681 ctggtccaga gcgtatcacc gccggtcgaa ggtaactctt tagctgccag aatcgcccct
     1741 gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt tgtcgttatc cggcggatta
     1801 tcgttatggg tatccagcac gatgatctcg tcaacccctt cggcatgagg gtaaacaggg
     1861 tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt gctgctgcgg cgtaatagct
     1921 tgatcgcgca gaaataccac ctgatagcgc agcaccgcat ggtaaagctg ttcaaactga
     1981 ttatcgctta acgggcgcgt caggtcggca cccgaaattt gtgcgccgat atacggcccc
     2041 agcggggtaa tactcagacg ttcactcatt gtacttctcc atgccagggc gtcagacggc
     2101 gctgtaacgc gcgcagaccc agttctaaaa gaaaggcgat aatcgcaatc accgcgatcc
     2161 ccgccagtac cacgtcagtc gcgagaaatt cacccgctga ctgaaccata aatcctaaac
     2221 cgcgtgtcgc ggcaatcagc tccgccgcta ccagcgtaga ccagcccaca cccagaccaa
     2281 tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa aatgacaaac cacagcacct
     2341 gcgcacggct ggcacccagc gactgggcgg cacgaatgcg aacctgctgc gcgcttttca
     2401 cccccgccag caccgacatc gccaccggtg caaaaatcgc taaatagatc aataagatct
     2461 tcgaggtttc accaatacca aaccagatca ccatcagcgg caaataagcc agcggcggca
     2521 ccggacgata aagttcgatt accggatcca gaatgccgcg caccgtcggg cttagcccca
     2581 tggcgatccc gaccggaata ccgatcacca ccgccgcgag tagcgccagc acaatgcgcg
     2641 tcaggctggc tgccagatgc tgccacagcg tggcgtccat aaatccctgc ggtccggcaa
     2701 tggtgagcag ttttgccagt acttgttgcg gcggcggcaa aaacagcggg ctaatcagtt
     2761 gcagcgccgc caccgtccac catacagtga gtaaaaccgc taacgtgcca atgcttaagg
     2821 tcacctgacg cgagagcggc cagcgccatt tcagccgccg cgaatgcagt ttttcattaa
     2881 tgagcacact catgagaacg cctcccgttg ctcaaatacg cggcttaaaa catattcgcg
     2941 catggcgatg aattgtggat cggacttgat gctgcggctc gactctcccg caacaaagcg
     3001 gcgagcaaag ttgagcggca gccgctccag cacacggcca gggccggatg aaagcagaac
     3061 cagttcagtc gccataaaca ccgcttcttc tatatcgtgg gtaatcaaca gcacctgctt
     3121 gcccgtctcc tgccagagtt tcagcagcag agtttgcatc tggtcgcggg tgaaggcgtc
     3181 cagcgcacca aacggttcgt cgagtaataa cagctgggga ttcgccgcca gcgcgcgggc
     3241 aatccccacc cgctgacgtt gaccaccgga aagctgccag atatagcgtt tttctgcgcc
     3301 ttccagcccc acttttttca gcatctggtg cgcgatttcc agtcgctgca ttttctctat
     3361 acctgccaat tgcaggccga acgccacgtt gtcctgtaca ttgcgccacg gtagtagccc
     3421 ttcattctga aaaaccaccc cgcgctctgc tcccggtccc tcaatacgct tacccgccag
     3481 ttgaatgctg ccatgctgat aaggcacaaa accggcaatc agattcagca gggtggtttt
     3541 accgcagccg gacggcccca gcaccaccag tagctcgccg ctttccagcg tcaggttgat
     3601 atcttccagt gccggtttgc cgccataatc ggcatagaga tgagagattt gcagcatctg
     3661 cgcctccttt tattgcacga agcgcgaggt aacgtactgg ctgtaatcat tcgctacagc
     3721 cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg atgattgctt tgttcaccgg
     3781 tccggtcagt tctgccgttt gttgctgcgg cgtcagatag gtattcccct tcaccagccc
     3841 cggaacgtca ccttccggca cgccgcttaa acgcgccagt ttgctgatgt tttccggctg
     3901 tttcagccac gcgtctgggt tagcaatgta cggttgctga gcatcgatgg cgcttttagc
     3961 gaacgctttc acgacctcag gatgtttctc ggcaaaatct ttgcgcacca cccagacgtc
     4021 cagcgttggc gcaccccact gcccgacctg ttcagaatcg gtcaacacct tgccgtcttt
     4081 ttccagggcg ttaaccgccg gtgcccagac ataagcacca tcaatatcac cccgctgcca
     4141 tgcggcgata atcgcgggcg gctgcaggtt cacaatctcc acttgccctg gtttaatccc
     4201 ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg gagataaacg gtacggcaat
     4261 gcgtttgcca atcaaatctt cgggtttgct gatagttttc tttaccacca gcgcttcgga
     4321 gttacccagt tttgacgcca gcaagaagac ttcaatcggc acctgttggc tggctgcaac
     4381 cgctaatggg ctggaaccga ggttgccgat ttgcacgtcg ccagaagcca gcgcccgcac
     4441 gatgctggct ccgctgtcaa acttacgcca gtccacgctc gccccgctct ctttagcaaa
     4501 ggtgttgtcg gcctgagcca ctttcgccgg ttcggctgag gtttgatacg ccacggtgac
     4561 gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg gcaagaagtg tgttacgcga
     4621 tgaaattgcc atgattgtct gctcccctgt cttgttatgg gagcagtatt caggaataaa
     4681 aacattcatt aaaagaatta gtcgttatcg cacagatgat tttattctta gcaaaaaaac
     4741 ggcaaaggaa agattaaata tgcaattact ccgttctaac aattgggaaa attactacac
     4801 ttcattttag tgatgcataa acaaccacat tacg
//
LOCUS       DAZPEP010000230         4834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZPEP010000230 DAZPEP010000000
VERSION     DAZPEP010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.232"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(108..551)
                     /locus_tag="ACHUT9_004082"
     CDS             complement(108..551)
                     /locus_tag="ACHUT9_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFR1350428.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            708..1637
                     /gene="metA"
                     /locus_tag="ACHUT9_004083"
     CDS             708..1637
                     /gene="metA"
                     /locus_tag="ACHUT9_004083"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFR1350429.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1906..3507
                     /gene="aceB"
                     /locus_tag="ACHUT9_004084"
                     /pseudo
     CDS             1906..3507
                     /gene="aceB"
                     /locus_tag="ACHUT9_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3537..4828
                     /gene="aceA"
                     /locus_tag="ACHUT9_004085"
                     /pseudo
     CDS             3537..4828
                     /gene="aceA"
                     /locus_tag="ACHUT9_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1205 a         1273 c         1225 g         1131 t
ORIGIN      
        1 gcagaaggcc atcctgacgg atggcctttt tgcattggcg cagaaaaaaa tgccgggtgc
       61 gacgctggcg cgtcttctcc agcctgggca ggcacggcag gggcagctta ttcctccaca
      121 tacgccagat tcagcaacgg atacggtttc cccaaatcgt ccacctcaga gcgtcccgta
      181 accttaaaac ccaccttctt atagaaccca accgcctgct cattttgctc attaacgttg
      241 gttgtcagtt ccggtgccat cgagagcgca tgctccacca gcatccgacc cacgccgcag
      301 ccgcacacat caggatcgat aaacagcgca tccatatgct gcccacttaa caacataaac
      361 ccaaccggct gatcccgttc attaaccgcg acccacaacg gcgcttccgg caggaaggaa
      421 cgaactagca cctccagctc ggcccgatac tctgctgata aaaaatcgtg tgtggcatcg
      481 acagaacgac accaaatcgc aacgagttcc tccccttcct catgccgtga gcggcgaata
      541 ctaataacca ttttctctcc ttttcgtcat tcttatattc taacgtagcc ttttccttga
      601 aactttctca ccttcaacat gcaggctcga cattggcaaa ttttctggtt atcttcagct
      661 atctggatgt ctaaacgtat aagcgtatgt agtgaggtaa tcaggttatg ccgattcgtg
      721 tgccggacga gctacccgcc gtcaatttct tgcgtgaaga aaacgtcttt gtgatgacaa
      781 cttctcgtgc gtctggtcag gaaattcgtc cacttaaggt tctgatcctt aacctgatgc
      841 cgaagaagat tgaaactgaa aatcagtttc tgcgcctgct ttcaaactca cctttgcagg
      901 tcgatattca gctgttgcgc atcgattccc gtgaatcgcg caacacgccc gcagagcatc
      961 tgaacaactt ctactgtaac tttgaagata ttcaggaaca aaactttgac ggtctgatag
     1021 ttaccggcgc gccgctgggc ctagtggagt ttaatgatgt cgcttactgg ccgcagatca
     1081 aacaggtgct ggagtggtcg aaagatcacg tcacctcgac gctgtttgtc tgctgggcgg
     1141 tacaggccgc gctcaatatc ctctacggca ttcctaagca aactcgcacc gacaaactct
     1201 ctggcgttta cgagcatcat attctccatc ctcatgcgct tctgacgcgt ggctttgatg
     1261 attcattcct ggcaccgcat tcgcgctatg ctgactttcc ggcagcgttg attcgtgatt
     1321 acaccgatct ggaaattctg gcagagacgg aagaagggga tgcatatctg tttgccagta
     1381 aagataagcg cattgccttt gtgacgggcc atcccgaata tgatgcgcaa acgctggcgc
     1441 aggaatattt ccgcgatgtg gaagccggac tagacccgga tgtaccgtat aactatttcc
     1501 cgcacaatga tccgcaaaac aaaccgcgcg cgagctggcg tagtcacggt aatttgctgt
     1561 ttaccaactg gctcaactat tacgtctacc agatcacgcc atacgatcta cggcacatga
     1621 atccaacgct ggattaatct tctgtgatag tcgatcgtta agcgattcag caccttacct
     1681 caggcacctt cgggtgcctt ttttatttcc gaaacgcacc tcagcaagtg aataaatttt
     1741 attcatattg ttatcaataa gttatcaagt atttttaatt aaaatggaaa ttgtttttga
     1801 ttttgcattt taaatgagta gtcttagttg tgctgaacga aaagcgcaca acgatccttc
     1861 gttcacagtg gggaagtttt cggatccatg acgaggagct gcacgatgac tgaacaggca
     1921 acaacaaccg atgaactggc tttcacaagg ccgtatggcg agcaggagaa gcaaattctt
     1981 actgccgaag cggtagaatt tctgactgag ctggtgacgc attttacgcc acaacgcaat
     2041 aaacttctgg cagcgcgcat tcagcagcag caagatattg ataacggaac gttgcctgat
     2101 tttatttcgg aaacagcttc cattcgcgat gctgattgga aaattcgcgg gattcctgcg
     2161 gacttagaag accgccgcgt agagataact ggcccggtag agcgcaagat ggtgatcaac
     2221 gcgctcaacg ccaatgtgaa agtctttatg gccgatttcg aagattcact ggccccggac
     2281 tggaacaaag tgatcgacgg gcaaattaac ctgcgtgatg cagttaacgg caccatcagc
     2341 tataccaatg aagcaggcaa aatttatcag ctcaagccca atccagcggt tttgatttgt
     2401 cgggtacgcg gtctacactt gccggaaaaa catgtcacct ggcgtggtga ggcaatcccc
     2461 ggtagcctgt ttgattttgc gctctatttc ttccacaact atcaggcact gttggcaaag
     2521 ggcagcggtc cctatttcta tctgccgaaa acccagtcct ggcaggaagc ggcctggtgg
     2581 agcgaagtct tcagctatgc agaagatcgc tttaatctgc cgcgcggcgc catcaaggcg
     2641 acgttgctga ttgaaacgct gcccgccgtg ttccagatgg acgaaatcct tcacgcgctg
     2701 cgcgaccata ttgttggtct gaactgcggt cgctgggatt acatcttcag ctatatcaaa
     2761 acgttgaaaa actatcccga tcgtgtcctg ccagacagac aggccgtgac gatggataaa
     2821 ccattcctga atgcttactc acgcctgctg attaaaacct gccataaacg cggtgctttt
     2881 gcgatgggcg gcatggcagc gtttattccg agcaaagatg aagagcgtaa taaccaggtg
     2941 ctcaacaaag taaaagcgga taaagcgctg gaagtcaata acggtcacga tggcacatgg
     3001 atcgctcacc caggccttgc ggacacggca atggcggtat tcaacgacat tctcggctcc
     3061 cgtaaaaatc agcttgaagt attgcgcgaa caagacgcgc cgattactgc cgatcagctg
     3121 ctggcacctt gtgacggtga acgcaccgaa gaaggtatgc gcgccaacat tcgcgtggct
     3181 gtgcagtaca tcgaagcgtg gatctccggc aacggctgcg tgccgattta tggcctgatg
     3241 gaagatgcgg cgacggctga aatttcccgt acctcgatct ggcagtggat ccatcatcaa
     3301 aaaacgttga gcaatggcaa accggtgacc aaagccttgt tccgctagat gctgggcgaa
     3361 gagatgaaag tcattgccag cgaactgggc gaagaacgtt tctcccaggg ccgttttgac
     3421 gatgccgcac gcttgatgga acagatcacc acttccgatg agttaattga tttcctgacc
     3481 ctgccaggct accgcctgtt agcgtaaacc accacataac tatggagcat ctgcacatga
     3541 aaacccgtac acaacaaatt gaagaattac agaaagagtg gactcaaccg cgttgggaag
     3601 gcattactcg cccatacagt gcggaagatg tggtgaaatt acgcggttca gtcaatcctg
     3661 aatgcacgct ggcgcaactg ggcgcagcga aaatgtggcg tctgctgcac ggtgagtcga
     3721 aaaaaggcta catcaacagc ctcggtgcac tgactggcgg tcaggcgctg caacaggcga
     3781 aagcgggtat tgaagcagtc tatctgtcgg gatggcaggt agcggcggac gctaacctgg
     3841 cggccagcat gtatccggat cagtcgctct atccggcaaa ctcggtgcca gctgtggtgg
     3901 agcggatcaa caacaccttc cgtcgtgccg atcagatcca atggtccgcg ggcattgagc
     3961 cgggcgatcc gcgctatgtc gattacttcc tgccgatcgt tgccgatgcg gaagccggtt
     4021 ttggcggtgt cctgaatgcc tttgaactga tgaaagcgat gattgaagcc ggtgcagcgg
     4081 cagttcactt cgaagatcag ctggcgtcag tgaagaaatg cggtcacatg ggcggcaaag
     4141 ttttggtgcc aactcaagaa gctattcaga aactggtcgc ggcgcgtctg gcagctgacg
     4201 tgacgggcgt tccaacccta ctggttgctc gtaccgatgc cgatgcagcg gatctgatca
     4261 cctccgattg cgacccgtat gacagcgaat ttattaccgg cgagcgtacc agtgaaggct
     4321 tcttccgtac tcatgcgggc attgagcaag cgatcagccg tggcctggcg tatgcgccat
     4381 atgctgacct ggtctggtgt gaaacctcca cgccggatct ggaactggcg cgtcgctttg
     4441 cacaagctat ccacgcgaaa tatccgggca aactgctggc ttataactgc tcgccgtcgt
     4501 tcaactggca gaaaaacctc gacgacaaaa ctattgccag cttccagcag cagctgtcgg
     4561 atatgggcta caagttccag ttcatcaccc tggcaggtat ccacagcatg tggttcaaca
     4621 tgtttgacct ggcaaacgcc tatgcccagg gcgagggtat gaagcactac gttgagaaag
     4681 tgcagcagcc ggaatttgcc gctatacctt cgtatctcac cagcaggaag tgggtacagg
     4741 ttacttcgat aaagtgacga ctattattca gggcggcacg tcttcagtca ccgcgctgac
     4801 cggctccact gaagaatcgc agttctaagc aacc
//
LOCUS       DAZPEP010000231         4830 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZPEP010000231 DAZPEP010000000
VERSION     DAZPEP010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4830
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.233"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(146..3367)
                     /gene="carB"
                     /locus_tag="ACHUT9_004086"
     CDS             complement(146..3367)
                     /gene="carB"
                     /locus_tag="ACHUT9_004086"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFR1350430.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3385..4533)
                     /gene="carA"
                     /locus_tag="ACHUT9_004087"
     CDS             complement(3385..4533)
                     /gene="carA"
                     /locus_tag="ACHUT9_004087"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFR1350431.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1069 a         1352 c         1246 g         1163 t
ORIGIN      
        1 tgtgagctaa atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat
       61 gggcaaaaaa aacggtcctc atcagagaac cgtattactt attcgatcaa attagcggat
      121 gaaaaatatc cgccatgaca cgctattatt tgatctgcgc gtgcatttcc tgcaccgaaa
      181 ttactttttc ggtcgcgtcg gcattcagcg ccatcgcggt agcgaaacca ccgttcaggg
      241 tggtgtcata atgcacttta tattgcagcg cactgcgacg gatcacgcgg gagtcttcaa
      301 tcgcgcgacg gcctgaagtg gtgttgatga tgtaggtata ttcgccattc ttgatgcggt
      361 cctgaatgtg cggacggcct tcatgcacct tgttcaccag acgcggatta atgcccgctt
      421 cgcccagcac aatcgccgtg ccgtgggtcg catccagctc gaagccctgt ttcagcagtt
      481 ttgccgccag gtccaccacg cgttctttat cgccttcgcg cacggaaagc aacgcacgac
      541 cgtgtttctt catggtggag ttgctgccca gctgcgcttt ggcaaacgct tcagcgaagg
      601 tgcggcccac gcccatcact tccccggtgg agcgcatttc tggccctaac agcgggtcaa
      661 cacccgggaa tttattgaac ggcagcacca cttctttcac cgagtagtac ggcgggataa
      721 cttctttggt tacgccctgc tcagccagcg atttgccagc catcacacgc gccgccactt
      781 ttgccagcgg tacgccggtg gctttggaga cgaacggaac ggtacgcgcc gcacgcgggt
      841 taacttcaat caggtagact tcgttgtttt tcaccgcaaa ctgcacgttc atcagaccgc
      901 gcacctgcaa ttcgaaggcc agtttctgca cctgctggcg catcacatcc tgaatttcct
      961 gacttaaggt gtaggctggc agagaacatg cggagtcacc ggagtgcacg cccgcctgct
     1021 cgatgtgctc catgatgccg ccaatcagca ccatttcgcc gtcgcagatg gcatccacgt
     1081 caacttctac cgcgtcatca aggaagtgat ccagcaacac tggcgcatcg ttagacacgc
     1141 tgaccgccgt ctggaagtag cgacgcaggt cagcttcgtc atagacgatt tccatcgccc
     1201 gaccgccgag aacgtaagac ggacgtacca ccagtgggta gccaatctct ttcgccttct
     1261 ctaccgccat ttcaatagcg gtaacggtgg cgttcgccgg ttgtttcagt ttcagacgct
     1321 caaccgcatg ctggaagcgt tcacggtctt ctgcacggtc gatagcatcc gggctggtgc
     1381 cgataaccgg tacgccagca gcttccagcg cgcgcgccag tttcagcggg gtctgaccgc
     1441 cgtactgaac gataacgcct ttcggcttct caatacgcac gatttccagc acatcttcca
     1501 gagttaccgg ctcgaagtag aggcgatcgg aagtgtcgta gtcggtggag acggtttccg
     1561 ggttacagtt aaccataatg gtttcgtaac cgtcttcgcg cagcgccagc gaggcgtgta
     1621 cacagcagta gtcgaattcg ataccctgac cgatacggtt cgggccgccg ccgagaacca
     1681 tgattttttc acggtcggta gacggattcg cttcgcactc ttcttcataa gtggagtaca
     1741 tgtaagcggt gtcggtggcg aactctgccg cacaggtatc cacgcgctta taaaccgggt
     1801 gcaggtcata ctggtcgcgc agcttgcgga tttccgcttc gcgcacgccc gccagttttg
     1861 ccagacgcgc atcggcaaag cctttgcgtt tcagctggcg caggaattca gcgttcaggc
     1921 cagtgatgcc cacttccgct actttctctt ccagacgcac cagttcttca atctgtacca
     1981 ggaaccagcg gtcaatattg gtcaggttga acacgccgtc cacagacagg cccgcacgga
     2041 aggcatcggc gatgtaccag atacgctctg cgccagcatc tttcagttcg cgacggattt
     2101 tggttaacgc ttccgggtca tcgaggctca ctttcgggtc gaatccagtc gcaccgactt
     2161 ccaggccgcg cagcgctttt tgcagggatt cctgctgcgt gcgaccaatc gccatcactt
     2221 cgccaaccga tttcatctga gtggtcagac ggtcgttagc accagcgaat ttttcgaagt
     2281 tgaagcgagg aattttagta accacatagt cgatggacgg ctcgaaggag gccggagtac
     2341 gtccgccagt gatgtcgttc atcagttcgt cgagggtgta acccaccgcc agtttcgccg
     2401 ccactttagc aatcgggaaa ccggtcgctt tcgacgccag cgccgaagaa cgggacacgc
     2461 gtgggttcat ttcgataaca atcaggcgac cgtttttcgg gttcaccgca aactggacat
     2521 tggaaccacc ggtttcaacg ccgatttcac gcagcaccgc catcgaggcg ttacgcatga
     2581 tttgatattc tttgtcggtc agcgtttggg ctggcgcgac agtgatggag tcaccggtat
     2641 ggatgcccat cgcatcaaag ttttcgatag agcagacgat gatgcagttg tcgtttttat
     2701 cacgcaccac ttccatctcg tactctttcc agccgatcag cgactcatca atcagcagct
     2761 ctttggttgg ggagagatcc agaccgcggg cgcaaatttc ttcaaactct tcgcggttat
     2821 aagcgatacc gccgccgcta ccgcccatgg taaaggatgg gcgaataatg cacgggaagc
     2881 ccacgtcagc ggcaaccgcc agcgcttctt ccatcgtgtg tgcgataccg gaacgcgcgg
     2941 tttccagacc aattttcttc atcgctacgt cgaaacgacg gcggtcttct gctttatcaa
     3001 tcgcatcggc ggtcgcacca atcatagtca cgccgaactc ttccaacacg ccctgacgct
     3061 ccagctccag cgcgcagttc agcgccgtct gaccgcccat cgttggcagc accgcgtccg
     3121 ggcgctcttt ttcaataatc ttgcgtacta cttcccagtg aatcggctcg atgtaggtcg
     3181 catcggccat ttccgggtcg gtcatgatgg tcgccgggtt ggagttcacc agaatgacgc
     3241 ggtaaccctc ttcgcgcagg gctttacatg cttgcgcgcc agagtagtca aactcacacg
     3301 cctgaccgat aacaatcggg cccgcaccca gaatcaggat actttttata tctgtacgtt
     3361 ttggcatggc tcttttactc ctgattactt agcggtttta cggtactgct caattaactc
     3421 gataaagtgg tcgaacaacg gcgctgcgtc gtgtggccca gggctggctt ccgggtgacc
     3481 ctggaagctg aacgccggtt tatcagtgcg atgaatgccc tgtaacgtac cgtcgaacag
     3541 ggatttatgc gtgacacgca ggtttgcagg taatgttgct tcatccaccg caaaaccgtg
     3601 gttctgggcg gtgatcatca ccacgttttt ctccacatct ttaaccggat ggttgccgcc
     3661 gtggtgacca aatttcattt tgacagtctt cgcaccgctc gccagcgcca gcagctgatg
     3721 accgaggcag atgccgaata ccggaatatc ggtttcgagg aatttctgga tggcggtaat
     3781 ggcgtaatcg cacggggccg ggtcgccagg accgttggag aggaagatgc cgtctggatt
     3841 cattttcagc acatcttccg cagaagtttg cgccggaacg atggtcaggc gacagcctct
     3901 atccaccagc atccgcagga tgttgcgctt ggcaccaaaa tcataagcca cgacgtggaa
     3961 cggcagctcg tcttcttttt tcgcttctgg caggccgccg gtcaacgtcc agctcccttg
     4021 tgtccagcta taggcttctg cggtggtcac ttcttttgcc agatccatgc cattcagacc
     4081 tgggaacgcg cgggcttttt ctaacgccaa cgccgcatcc gggttatcgc ctgcgataat
     4141 gcagccattc tgtgcgcctt tctcgcgcag taaacgcgtc agcttacggg tatcgatatc
     4201 ggcaatcgcc acgatgttat ggcgcttcag gtaagaagag aggtcttcgg tattacggaa
     4261 gttgctggca atcagcggca gatcgcgaat caccagacct tgtgcatgta cctgagaaga
     4321 ttcttcatcg gcgtcattag tgccgacatt gccaatatgg ggataagtaa gagtaacgat
     4381 ttgacgagaa taggaaggat cagtgaggat ttcttgataa ccggtcattg aagtattgaa
     4441 aacgacttcc ccaaccgccg aacctgttgc ccctatggcc cgaccgtgaa actgggttcc
     4501 gtcttccaga accaatagcg ctgacttaat caaaacaccc tccagagaat attcactcac
     4561 tttatttgca tattaattca caatgatgac gtaaatcaat gcaaatctgc ttaccgacga
     4621 atttctggca aacggcggca ttctggagat atgggcgcta aaagtcaact taatggcggt
     4681 attttttata ttattttgtg gctcatgcat gttttacagg cattaagcga caaaaagatc
     4741 atataacctg ttctggaaaa cgcttgcgca gcaagaagat tgcatgaaat aaaaacgagc
     4801 aggaaaaagt ggaccaaatg gtcaaaattt
//
LOCUS       DAZPEP010000232         4805 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZPEP010000232 DAZPEP010000000
VERSION     DAZPEP010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.234"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(46..2115)
                     /gene="glyS"
                     /locus_tag="ACHUT9_004088"
     CDS             complement(46..2115)
                     /gene="glyS"
                     /locus_tag="ACHUT9_004088"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFR1350432.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2125..3036)
                     /gene="glyQ"
                     /locus_tag="ACHUT9_004089"
     CDS             complement(2125..3036)
                     /gene="glyQ"
                     /locus_tag="ACHUT9_004089"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFR1350433.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3131..3430)
                     /locus_tag="ACHUT9_004090"
     CDS             complement(3131..3430)
                     /locus_tag="ACHUT9_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFR1350434.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3605..4600
                     /gene="wecH"
                     /locus_tag="ACHUT9_004091"
     CDS             3605..4600
                     /gene="wecH"
                     /locus_tag="ACHUT9_004091"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFR1350435.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4642..>4805)
                     /locus_tag="ACHUT9_004092"
     CDS             complement(4642..>4805)
                     /locus_tag="ACHUT9_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1350436.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         1096 a         1265 c         1221 g         1223 t
ORIGIN      
        1 ataaaaaaag cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga
       61 aatatccgca acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag
      121 acgcaattct ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc
      181 acgcagctct gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc
      241 cagcttgtca cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag
      301 ggtagaggca ttcacgcggt cgctcagcac ttcgtcagat ttcgccagaa tgttagatac
      361 acgtttgttc gccgccgcca gtgcagcagc tgcatccagg gtacggaaat gcgataccgc
      421 tttcatacgg gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat
      481 ggtgtcaacg gtgtaacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc
      541 gataacatca tcaactacgt tggcgttagt cagcttatcg ccatacagac gcactgcttc
      601 ttcggtcagc gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc
      661 aagtgcggca cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc
      721 gaagataccc gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt
      781 ggacggcagg tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac
      841 gtcttccgct tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt
      901 gaactcgaag accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc
      961 gtggttaacg tcagcgccaa tctgttcagc aatccagcca gccagcgcct ggatgcggtc
     1021 agttttgtca cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag
     1081 gttatcttcc agacgttttt tacggtcggt attgaagaag aactcggcat ccgccagacg
     1141 tggacgaacg actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt
     1201 ggcaacgaag atgaagttcg gcagcagttt gccatcgttc gcatacaccg ggaagtattt
     1261 ctggtcacct ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc
     1321 gaattttgcg gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct
     1381 ttcgcttaag tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat
     1441 cttcgcttta cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg
     1501 atactgatcg gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat
     1561 cacgcgatcg gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag
     1621 ggtcacggtg tgcaccggac gcacgaagtg cacgtcgctt gcgccccaac gcatcagttt
     1681 cgggatcggc agtttcgcca gcgaagtcgc aaccatattc ggcagtagtg cttcagtgct
     1741 ttcgcccttc acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc
     1801 agcctggtca acggtaatac cgcaaccacg cgcccaacct tctgccgctt tgctcggttt
     1861 gccttcagcg tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg
     1921 ttgcgcttcc gccaggttag ctactttcaa cgccagacga cgcggagcag caaaccattg
     1981 aacggtgccg tgtgcgaggc cagcgttatc cagctccgca gtaaagttcg cagcaaagga
     2041 ctcagccagg ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa
     2101 agttttctca gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc
     2161 ttcacgggaa gcgtagtatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat
     2221 gtagcgctga cgctcagtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg
     2281 ggcggctttc agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag
     2341 ctgctgcgct tctttctcgt actgctcgaa gcaggtgaac aggaaatcca catccgcgta
     2401 ttcgaagttg taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt
     2461 tttacccagc gggccgtcgc tccagaccag gtcgtaaacg ctgtctacgc cctgaatgta
     2521 catggccaga cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc
     2581 accaacctgc tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca
     2641 gcccagtccc caggcaccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc
     2701 gtgaatagtc gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt
     2761 gtccggcgat ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt
     2821 ttcgccgtag cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg
     2881 ttctggcccc agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat
     2941 gtccaatggt tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat
     3001 caagccctgg aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg
     3061 gatttaaagg aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa
     3121 aaagcagggc ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga
     3181 caaactgctc tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga
     3241 aggcggtgac gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc
     3301 ataacgcttc catattcaac gaacgcagag gatcaacctt tacccgttgt gctttctgca
     3361 cgggtgacgg tgtagaccaa cccactagca ccataagcgc cattgccgga aagaaagcgt
     3421 tcatcatcat taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag
     3481 tggctgatat cttacgaatt tccttaagcc tctataagct gtagtgataa aactcagatt
     3541 attccagtgc gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac
     3601 acagatgcag cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt
     3661 ggtgatgatt cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac
     3721 atgggatatc gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat
     3781 gatttccggc tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat
     3841 cggcttatgt ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc
     3901 catcaatatg gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg
     3961 gtttttcttc gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt
     4021 cggcggaaaa atgttgctgg tgctaatggc ggtgattggc attatcgcta acccaaacac
     4081 agtgccgcag aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga
     4141 tactttttac tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca
     4201 gcataaagca ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc
     4261 tcgcgggaca ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta
     4321 ctgtgggccg atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct
     4381 ggatacgcgt accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg
     4441 attccacgcc ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc
     4501 aatactggat attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat
     4561 gctggtacaa cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg
     4621 gactgaccgg gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt
     4681 tggcgggttt attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct
     4741 taagaagaaa gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag
     4801 accta
//
LOCUS       DAZPEP010000233         4803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZPEP010000233 DAZPEP010000000
VERSION     DAZPEP010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.235"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            6..81
                     /locus_tag="ACHUT9_004093"
     tRNA            6..81
                     /locus_tag="ACHUT9_004093"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="ACHUT9_004094"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="ACHUT9_004094"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="ACHUT9_004095"
     CDS             complement(299..1057)
                     /locus_tag="ACHUT9_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFR1350437.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="ACHUT9_004096"
     CDS             complement(1065..2168)
                     /locus_tag="ACHUT9_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFR1350438.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="ACHUT9_004097"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="ACHUT9_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4459)
                     /locus_tag="ACHUT9_004098"
                     /pseudo
     CDS             complement(3427..4459)
                     /locus_tag="ACHUT9_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
BASE COUNT         1317 a         1241 c         1147 g         1098 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcattc agcatggcga
     3721 aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact accggcccaa
     3781 gcggttcttt agagataacc tccggtaaga caatccattc agctgggttg cttaatttga
     3841 tgcgcagggc atacagttgc gactgatccg aggccagcgt atcgcagcga ccagattcca
     3901 gtgccttcgc tgattcgtca gagcgatcga aagtcaccgg tgtgtacttc atattgtttg
     3961 ccttgaaata gtcggcgacg ttgagttcgg tatctgtacc cgcctgaata cagacggtag
     4021 cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg aagccaatgc
     4081 cgtcgtaatc agtgacgcca gtaaatgcca ttcccatccc ggcatcgcga gatgaagtcc
     4141 aggtcgtatt acgggagagc aaatccacct cccctgactg taaagcggtg aagcgttctt
     4201 ttgcagtgag cggggtatat ttcactttcg tgtcgtcacc aaatacagca gcggcaacac
     4261 cacgacaaac atctacatca atacctgaaa acttaccgtc tgcatcggca taagagaacc
     4321 caggtaatcc atcactgatc ccgcattgca caaaaccttt tttctgtacg gcatcaagcg
     4381 tcgcgccagc atgcgcctga tttgcaacgg caagcagcac gctggcggca gccagtgtgg
     4441 ctatcatcat ctttttcata atgcttcctg tgaggcgaaa attatcgtta taggacgttt
     4501 gggaaacgtc ttttacctgt ctgtggccat cctcttataa gcaaaagtag tgccatcttt
     4561 tgcgcacgat ttcgcgtcgc aataagctga gtgtagacag gaaataataa aaacagcgcc
     4621 ccgcaatggt gcgaaaatga aacatgcgcc acattttggc gcaaaatatt tacgcgtgtt
     4681 ttactaagca gaataatgat tgttagcgtg agtatatttc tggtgggagg tgtgaaagtt
     4741 aaattgatgc gttatttcga aagcagcgaa ataacgcgat ataacatgct aaggtaatga
     4801 ctc
//
LOCUS       DAZPEP010000234         4765 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZPEP010000234 DAZPEP010000000
VERSION     DAZPEP010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.236"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            59..1003
                     /gene="leuO"
                     /locus_tag="ACHUT9_004099"
     CDS             59..1003
                     /gene="leuO"
                     /locus_tag="ACHUT9_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFR1350439.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            complement(1158..1202)
                     /locus_tag="ACHUT9_004100"
     CDS             complement(1158..1202)
                     /locus_tag="ACHUT9_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350440.1"
                     /translation="MKRNDKILHYEGLN"
     gene            1321..3045
                     /gene="ilvI"
                     /locus_tag="ACHUT9_004101"
     CDS             1321..3045
                     /gene="ilvI"
                     /locus_tag="ACHUT9_004101"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFR1350441.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3048..3539
                     /gene="ilvN"
                     /locus_tag="ACHUT9_004102"
     CDS             3048..3539
                     /gene="ilvN"
                     /locus_tag="ACHUT9_004102"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFR1350442.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            3551..3637
                     /locus_tag="ACHUT9_004103"
     CDS             3551..3637
                     /locus_tag="ACHUT9_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFR1350443.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            3719..4723
                     /gene="cra"
                     /locus_tag="ACHUT9_004104"
     CDS             3719..4723
                     /gene="cra"
                     /locus_tag="ACHUT9_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFR1350444.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
BASE COUNT         1156 a         1092 c         1276 g         1241 t
ORIGIN      
        1 attatttctc tgcattccaa taagggaaag ggagttaagt gtgacagtgg agttaagtat
       61 gccagaggta caaacagatc attcagagac ggcggagtta agcaagccac agctacgcat
      121 ggtcgatctc aacttattaa ccgttttcga tgccgtgatg caggagcaaa acattacccg
      181 tgccgctcat gttctgggta tgtcgcaacc tgcggtcagt aacgctgttg cacgcctgaa
      241 ggtgatgttt aatgacgagc tttttgttcg ttatggccgt ggtattcaac cgactgctcg
      301 cgcatttcaa ctttttggct cagttcgtca ggcattgcaa ctagtacaaa atgaattgcc
      361 tggttcaggt tttgaacccg cgagcagtga acgtgtattt catctttgtg tttgcagccc
      421 gttagacagc attctgacct cgcagattta taatcacatt gagcagattg cgccaaatat
      481 acatgttatg ttcaagtctt cattaaatca gaacactgaa catcagctgc gttatcagga
      541 aacggagttt gtgattagtt atgaagactt ccatcgtcct gaatttacca gcgtgccatt
      601 atttaaagat gaaatggtgc tggtagccag caaaaatcat ccaacaatta agggcccgtt
      661 actgaaacat gatgtttata acgaacaaca tgcggcggtt tcgctcgatc gtttcgcgtc
      721 atttagtcaa ccttggtatg acacggtaga taagcaagcc agtatcgcgt atcagggcat
      781 ggcaatgatg agcgtactta gcgtggtgtc gcaaacgcat ttggtcgcta ttgcgccgcg
      841 ttggctggct gaagagttcg ctgaatcctt agaattacag gtattaccgc tgccgttaaa
      901 acaaaatagc agaacctgtt atctctcctg gcatgaagct gccgggcgcg ataaaggcca
      961 tcagtggatg gaagagcaat tagtctcaat ttgcaaacgc taactgaatg cagaataggt
     1021 cagacatgaa tgtctggttt attctgtatt ttttattgaa tgtagaattt tattctgaat
     1081 gtgtgggctc tctattttag gattaattaa aaaaatagag aaattgctgt aagttgtggg
     1141 attcagccga tttattatca atttaatcct tcgtaatgga ggattttatc gtttcttttc
     1201 acctttcctc ctgtttattc ttattaccct gcgtttatgt ctctggctgc caattgctta
     1261 agcaagatcg gacggttaat gtgttttaca cattttttcc gtcaaacagt gaggcaggcc
     1321 atggagatgt tgtctggagc cgagatggtc gtccgatcgc ttatcgatca gggcgttaaa
     1381 caagtattcg gttatcccgg aggcgcagtc cttgatattt acgatgcatt gcataccgtg
     1441 ggtggtatcg atcatgtttt agttcgtcat gagcaggcgg cagtgcatat ggccgatggt
     1501 ctggcgcgcg cgaccgggga agtcggcgtc gtgctggtaa cgtcgggtcc aggggcgacc
     1561 aatgcgatta ctggcatcgc caccgcttat atggattcca ttccattagt tgtcctttcc
     1621 gggcaggtag cgacctcgtt gataggttac gatgcctttc aggagtgcga catggtgggg
     1681 atttcgcgac cagtggttaa acacagtttt ctggttaagc aaacggaaga cattccgcag
     1741 gtgctgaaaa aggctttctg gctggcggca agtggtcgtc ctggaccagt agtcgttgat
     1801 ttaccgaaag atattcttaa tccggcgaac aaattaccct atgtctggcc ggagtcggtc
     1861 agtatgcgtt cttacaatcc cactactacc ggacataaag ggcaaattaa gcgcgctctg
     1921 caaacgctgg tagcggcaaa aaaaccggtt gtctacgtag gcggtggggc aatcacggcg
     1981 ggctgccatc agcagttgaa agaaacggtg gaggcgttga atctgcccgt tgtttcctca
     2041 ttgatggggc tgggagcgtt tccggcaacg catcgtcagg cactgggcat gctgggaatg
     2101 cacggtacct acgaagccaa tatgacgatg cataacgcgg atgtgatttt cgccgtcggg
     2161 gtacgatttg atgaccgaac gacgaacaat ctggcaaagt actgcccaaa tgccactgtt
     2221 ctgcatatcg atattgatcc tacttccatt tctaaaaccg tgactgcgga tatcccgatt
     2281 gtgggggatg ctcgccaggt cctcgaacaa atgcttgaac tcttgtcgca agaatccgcc
     2341 catcaaccac tggatgagat ccgcgactgg tggcagcaaa ttgaacagtg gcgcgctcgt
     2401 cagtgcctga aatatgacac tcacagtgaa aagattaaac cgcaggcggt gatcgagact
     2461 ctttggcggt tgacgaaggg agacgcttac gtgacgtccg atgtcgggca gcaccagatg
     2521 tttgctgcac tttattatcc attcgacaaa ccgcgtcgct ggatcaattc cggtggcctc
     2581 ggcacgatgg gttttggttt acctgcggca ctgggcgtca aaatggcgtt gccagaagaa
     2641 accgtggttt gcgtcactgg cgacggcagt attcagatga acatccagga actgtctacc
     2701 gcgttgcaat acgagttgcc cgtactggtg gtgaatctca ataaccgcta tctggggatg
     2761 gtgaagcagt ggcaggacat gatctattcc ggccgtcatt cacaatctta tatgcaatcg
     2821 ctacccgatt tcgtccgtct ggcggaagcc tatggtcacg tcgggatcca gatttctcat
     2881 ccgcatgagc tggaaagcaa acttagcgag gcgctggaac aggtgcacaa taatcgcctg
     2941 gtgtttgttg atgttaccgt cgatggcagc gagcacgtct acccgatgca gattcgcggg
     3001 ggcggaatgg atgaaatgtg gttaagcaaa acggagagaa cctgattatg cgccggatat
     3061 tatcagtctt actcgaaaat gaatcaggcg cgttatcccg cgtgattggc cttttttccc
     3121 agcgtggcta caacattgaa agcctgaccg ttgcgtcaac cgatgatccg acattatcgc
     3181 gtatgaccat ccagaccgtg ggcgatgaaa aagtacttga gcagatcgaa aagcaattac
     3241 acaagctggt cgatgtcttg cgcgtgagtg agttggggca gggcgcgcat gttgagcggg
     3301 aaatcatgct ggtgaaaatt caggccagcg gttacgggcg tgacgaagtg aaacgtaata
     3361 cggaaatatt ccgtgggcaa attatcgatg tcacaccctc gctttatacc gttcaattag
     3421 caggcaccag cgataagctt gatgcatttt tagcatcgat tcgcgaggtg gcgaaaattg
     3481 tggaagttgc tcgttctggt gtggtcggac tttcgcgcgg cgataaaata atgcgttgag
     3541 aatgatctga atgcgcaatt tgcagcccaa catgtcacgt tgggcttttt ttgcgaaatc
     3601 agtgggaacc tggaataaaa gcagttgccg cagttaattt tatgcgctta gatgttaatg
     3661 aatttaaccc ataccagtac aatggctatg gtttttacat tttacgcaag gggcaattgt
     3721 gaaactggat gaaatcgctc ggctggcggg agtgtcgcgg accactgcaa gctatgttat
     3781 taacggcaaa gcgaagcaat accgtgtgag cgacaaaacc gttgaaaaag tcatggctgt
     3841 ggtgcgtgag cacaattacc acccgaacgc cgtggcagct gggcttcgtg ctggacgcac
     3901 acgttctatt ggtcttgtga tccccgatct ggagaacacc agctataccc gcatcgctaa
     3961 ctatcttgaa cgccaggcgc ggcaacgggg ttatcaactg ctaattgcct gctcagaaga
     4021 tcagccagac aacgaaatgc gctgtattga gcacctttta cagcgtcagg tcgatgccat
     4081 tattgtttcg acgtcgttgc ctcctgagca tcctttttat caacgctggg ctaacgaccc
     4141 gttcccgatt gtcgcgctgg accgcgccct cgatcgtgaa cacttcacca gcgtggttgg
     4201 tgccgatcag gatgatgccg aaatgctggc ggaagagtta cgtaagtttc ccgccgagac
     4261 ggtgctttat cttggcgcgc tgccggagct ttctgtcagc ttcctgcgtg aacaaggttt
     4321 ccgtactgcc tggaaagatg atccgcgcga agtgcatttc ctgtatgcca acagctatga
     4381 gcgggaggtg gctgcccagt tattcgaaaa atggctggaa acgcatccga tgccgcaggc
     4441 gctgttcaca acgtcgtttg cgttgttgca aggggtgatg gatgtcacgc tacgtcgcga
     4501 tggtaaactg ccttctgacc tggcaattgc cacctttggc gataacgaac tgctcgactt
     4561 cttacagtgc ccggtgctgg cagtggctca acgtcaccgc gatgtcgcag agcgtgtgct
     4621 ggagattgtc ctggcaagcc tggacgaacc gcgtaagcca aaacctggtt taacgcgcat
     4681 taaacgtaat ctctatcgcc gcggtgtgct cagccgtagc taagccgcga acaaaaatac
     4741 gcgccaggtg aatttccctc tggcg
//
LOCUS       DAZPEP010000235         4762 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZPEP010000235 DAZPEP010000000
VERSION     DAZPEP010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4762
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.237"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..678
                     /locus_tag="ACHUT9_004105"
     CDS             <1..678
                     /locus_tag="ACHUT9_004105"
                     /inference="COORDINATES: protein motif:HMM:NF017666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFR1350445.1"
                     /translation="KFLFLMSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGA
                     ATEHDTLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTS
                     YWLRHIYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQM
                     VCCADTLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSY
                     RIAQRLS"
     gene            1042..1928
                     /locus_tag="ACHUT9_004106"
                     /pseudo
     CDS             1042..1928
                     /locus_tag="ACHUT9_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049841.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1928..2218
                     /locus_tag="ACHUT9_004107"
     CDS             1928..2218
                     /locus_tag="ACHUT9_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350446.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            2539..2829
                     /locus_tag="ACHUT9_004108"
     CDS             2539..2829
                     /locus_tag="ACHUT9_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350447.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHTALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            2829..3248
                     /locus_tag="ACHUT9_004109"
     CDS             2829..3248
                     /locus_tag="ACHUT9_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350448.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYVKSLINKDN"
     gene            3416..3739
                     /locus_tag="ACHUT9_004110"
     CDS             3416..3739
                     /locus_tag="ACHUT9_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350449.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNAENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            4169..>4762
                     /locus_tag="ACHUT9_004111"
     CDS             4169..>4762
                     /locus_tag="ACHUT9_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350450.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYACFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1554 a          878 c          977 g         1353 t
ORIGIN      
        1 aaatttttat ttcttatgtc aattgagcat attttaatcg ggaagcatca tgggggttct
       61 gattactgga gaactccatt tttgttattt aagaacttac accgtgaatt tattttcagt
      121 ctagatggtg ctgcaactga gcatgacacg ctgttaccac gattcaccga tgatataagc
      181 cgtcaatcat gggttagtga aaaggtcttc tgtaacccgc cgtacagcga tattccgtcg
      241 ttcctcttaa aagcgtctga agcggattta gtagctttcc tgataccgca tagagccaac
      301 accagctact ggcttaggca catttacagc aataaccact gtcatgaaat ccgtattctt
      361 catcgtgcag ttaaatacct tccaccagca gggcataaca ggcttactat taggtcaccg
      421 tttccaagtg cagtcgttgt ttttaaaaaa gaaccacgta agcatgaaat tacacagatg
      481 gtttgctgtg ctgatacatt gctaccgtta accatcatta accgtggtgg actaagagga
      541 agacccacca tttacccacc tgagacgctc gacagcttca taaaactata caggcaagga
      601 aaacccatta agtacattgc agatgctctc agaatgcctc tgagcacgtc atacaggatt
      661 gctcaaaggt tgagctaaaa ttctcataaa tatggtcatt tacaagacag gtgattcaga
      721 tgttaagaag tatccctgtg aacaataaat gaccaatcag taatcattct gtgaaataat
      781 tgttgcaatt tataatcaca tctgttatag tttctatatg ggctgataaa aaagttcatg
      841 tcttgaagtg gtaacacttg gggaggtgtc agatggttgg cgtatattaa cctctcattc
      901 agggtatacc tcagcgtttt aatcatcgta ctgtttcagg catggacttc tttatcagtc
      961 catcaatccc ccagatattt taatcacaca ggctaatcgg ttttatccgg ttggcctttt
     1021 cacgtatgag gtaatttatg aatgtctaaa tataacccaa atcagtttta taaatttgat
     1081 gtgcgaatct ctcaactttc agaagaaatt aacgcagcag caagattgat ttatatggtc
     1141 atggtccata gctgtgactt ctatgaagag aaatacaaat catattcacc aagtattgac
     1201 catctcgctt atatggctgg tgtatcaaag agaacagcaa tcagagccat tgagacactt
     1261 gagaaacacg gtttaattca ggtgcaaaag aatcaggggc gtcaaagcac ttatcgtgta
     1321 ttcacactgg atgataagcc ggaattactt gtaagaccta catcacagga agttgcagcc
     1381 tcagtaagac aaaatgacac cactaccagt gacaaaatgt catgggggta gtgacatttt
     1441 gtcacccgtg ttagagagat aagttattag ataaaacatt agataataat attacagata
     1501 agaataaaga gactggactt aatgatgata ttaaagaaga tgactttctt tctttggatg
     1561 aattaaatga ttttctctct atgaatgaat ctgaccttga agtaagctct aaaaagactt
     1621 ctaaacaaga cagagaaatc actgatgtga gtgttgatag tgaccataaa aacacacagt
     1681 gccccgccac tgatgatgag ttggacccct tcgatgatga agaggatgaa cgtgaaaaag
     1741 ccattaagag ggcagagaag aaaaagagat tcgagcttgc aagacaaaag aacaaagtat
     1801 cgggtttaga cccattcgat gacagtgaag atgattatta tctttcaccg aaaaagaaac
     1861 aaactcagga tgacaagaag caaaaaatta aaccgaagat gtattttgcc ggtggaggaa
     1921 ttaagtaatg aaagaatatc acggtgaaaa gagatacaaa gattacctgt taaagcgtta
     1981 cagcatttct cgtgaaggtc atcttatgaa ggatactcac ggggaagttt accggattag
     2041 accaaagaaa gaagggaggg gttatttctt ttacgatggt gtgacagatt taaagattga
     2101 cgctttgagg tttgcagtgc tgtatcattt tgatatctgg aattcaatcc accagctacg
     2161 attaaaagac ggtgacccca gcaacttaaa ggccaccaat attatcacaa aacgttagtt
     2221 tgttttacca tcatcaatcg taattgtgat ttcatatttg aaaagcttaa tgctgaaatt
     2281 gaatcgtgta cttctggcga taatcatttt taaaagctga tacacaagaa gcaccagaaa
     2341 cacaaagcaa cctaagcttg cgaatctgtg agattcagca attgtttcaa gaaagcccat
     2401 taattcccct tatttatcaa agggaatgac ctgtatcagc cccctacaac catattaaca
     2461 tgtaaatgag ttgttgacaa tatgttataa atatgttaaa gttagttata agtcaaagat
     2521 tttaagaagg aaaatttaat gagacacaca gttacacgcc ttttgccaga ggcatattcc
     2581 agactggcag caatcaaaga caaataccca gccatgagcc acacagcact tgttggtgag
     2641 attctgaagg aacacacagc ccttacaccg tttgacgcta tcaagggcgt tgcagagggt
     2701 tctaaggcca tcagaatcac aacgggccaa ctggaactac tcaaagcaca gtccgcagtg
     2761 gcaggttgct caatgattca ggtgttgagt gccaaggttc atcaactggc ggataagctg
     2821 ggggtttaat gctatgcaca tttgattatc atgcgtttta tcgccataga ggtcaccttg
     2881 tccgtgagaa taacggaaca tggaaagatt taactcgtaa tgctgaaccc ttcacacacc
     2941 agcaattaaa atcccgcttc tccagatgga agaatgcacc agcagatact ggtgattttg
     3001 agattatcca taccttcgat tatgcctcag aagatgacct tcacgatgca attaagctga
     3061 ttgaagactt caatacctca gaagatgtgg atggtgacaa atataaatcc cgtggtaacc
     3121 gctataaccg tgatgatatc actcaggcca taaatgctgg tcaatctcag cgatacatta
     3181 cagaaaaata tggtatttca cggagacagt atttctacgt taagtcattg attaataaag
     3241 acaattaaaa ataaccatct aagaaacggt agaatgcact ttacaggcag agttgcacct
     3301 taatttgata aattacctta ccagccagtg caactccctc tgtaacccgc atggttactg
     3361 gcctcagaat ggctatttta agaggtctga ggtgtgtttc ataggagatt taaaaatgaa
     3421 taattaccca atgcaaatta tggttgataa cgacactgct atgatggtgc aaagcttcat
     3481 tgatgcaggt atcgcaatta attttgataa attactaaag ctgatggctg ataatgcaga
     3541 aaacatctct gactttattc agtcggtgga gtttaacgag cctcgaatga tgctaccaat
     3601 tacagacagc aacatgaaaa gattggttat cgagcaaacg aacatataca gtatttcccc
     3661 agagcaattt ttgaaaggtg ctgtgacaat cctgtacagt gataatattc tggtgacgga
     3721 ttctgtgagg gttcactaat gggtttaata attgcttgga cgttgcttgg cttatttgtt
     3781 ggttgcatta ttaacaatgc ggtaaataag aattgatgtt atcaacctgt taaaaagctg
     3841 ttgacaaata accttaagca tggcataatt ttaattatgg gagagcaatt ctctcttatt
     3901 tttctttcaa taatccgatg gatggatatt ctgagttgga aaggtgcaat gcttttcgtc
     3961 atggcctttc aggttagtct gagaggctat ctcaaaaagt actcttgttc attcattatt
     4021 gaatcctttg cccttcacag aaatgtttag ggctttttca gtttttaaaa gcacacttgg
     4081 caggttcttg ttcagtcaga ctcttcacct ctgattgctg agtgtgcttc tataaattga
     4141 aataacaaat aaactaaggg gtgaacgaat gattagaaat gaacatgaaa tgcttatcga
     4201 acacagacca agtgcacttg atattcacga attaactttg cgtgaatctt ttgatagagc
     4261 ttggcgggaa tcacacgaag taaataaagg tccatatatt ccaccagaac cactcgagat
     4321 tccaagagtt gaaattgatt tttctatgaa cgaacggtgt gaacttgatt tgaagttgaa
     4381 gctacaacgc cgatatttcc aagctattaa agactctcaa gaagcaatga gaagtgttta
     4441 cgagaaaatt cagtccaagc ttgaagacga aattgacgaa atggttattt cgatggagct
     4501 tcaaaccaat tcttatgctt gtttctctcg actagaccgt gacggttggg ggtatgaccc
     4561 tgtagagatt ggtaaagtga ttgcagattt accagaaggt caaagagttg ttcgtacaga
     4621 aagagctgtc agagcaccac atcgactgtc tctaattgtt gctgataaat cacaagctga
     4681 gcttgaagaa attgccaaag agaaactgat gaaagcaaga gaaaatgaga ttaatttgct
     4741 gaaatcaaga ctggctaagc tg
//
LOCUS       DAZPEP010000236         4721 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZPEP010000236 DAZPEP010000000
VERSION     DAZPEP010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.238"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            248..1117
                     /locus_tag="ACHUT9_004112"
     CDS             248..1117
                     /locus_tag="ACHUT9_004112"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFR1350451.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1277..1870)
                     /gene="rclC"
                     /locus_tag="ACHUT9_004113"
     CDS             complement(1277..1870)
                     /gene="rclC"
                     /locus_tag="ACHUT9_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFR1350452.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1882..2118)
                     /gene="rclB"
                     /locus_tag="ACHUT9_004114"
     CDS             complement(1882..2118)
                     /gene="rclB"
                     /locus_tag="ACHUT9_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFR1350453.1"
                     /translation="MFKKSVLFATLLSGVMEFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            complement(2227..3552)
                     /gene="rclA"
                     /locus_tag="ACHUT9_004115"
     CDS             complement(2227..3552)
                     /gene="rclA"
                     /locus_tag="ACHUT9_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFR1350454.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            3778..4632
                     /gene="rclR"
                     /locus_tag="ACHUT9_004116"
     CDS             3778..4632
                     /gene="rclR"
                     /locus_tag="ACHUT9_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFR1350455.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
BASE COUNT         1303 a         1027 c         1048 g         1343 t
ORIGIN      
        1 acattctcca ttaattcttc gtgatatcgc acattttcgt tatgcccagg gccctaagtg
       61 tatcaaaaac agcaaggtcg aaggcaaaag cactggtagc gtctgcttcg ttgtccccga
      121 aaatagagag gtttcagtcc tacattatta atgaattttt tgcataagtg atatcaaaat
      181 ccacatacta atttgaggtt atgttttaac gtagactcat tgttcatgcc taatggaggg
      241 actgacagtg gaattttccg tattaagtaa caatctgaaa atgccgatga tgggatttgg
      301 tgtttttcag gttaccgata aaaacgtgtg caaacagtca gtgttgaacg ctatccgcac
      361 gggttatcga ctcatcgaca ctgcagcggt atacggtaat gaagatgccg tcggtgaagc
      421 cgttcgtgaa gctatatctg aaggtttatg tacccgagat gagttattta taacgtctaa
      481 attgtggggg caggatatgt tgaatcagga tacggcagca gcaggtattg aagcatcatt
      541 aaaaaaatcg ggactagagt acttcgacct ctatttattg caccaggcta tgcgcgatta
      601 tttcagtgtg tggcgtgcac tcgaagatgc ctatgaagag ggcaaattaa aagcaattgg
      661 ggtttccaat ttctatcctc atgttctggc gaacttttgt gaaacggtaa gagttaaacc
      721 gatggtcaac caggtcgagt tgcatcccta ttttgcccaa ccagaggcgc tggcaaccat
      781 gaagtattat aacgtgcagc ctgaagcatg ggctccgtta ggtggtggac gacataaacc
      841 ctttgaaaat aatctgcttc agagtattgc agatgcccat caaaaatcga tttctcaagt
      901 cattctgcgt tggaatattc aacggggagt ggtcgttatt ccgaaatcga cacatcaaca
      961 gcgtatcgaa gaaaattttg ctatctggga tttctcactg acagagaaag aaatggcaca
     1021 aattagttcg cttgatttgg gttatgttgg ggagtcggta aaacatttta atcctgaatt
     1081 tgttcgtggt tgtcttgctg taaaaataca tgattgatat taatcatata ttttacctga
     1141 gacgacaaga atcttttaac aggggagtga tattgatctt cactctgtca tatctccggt
     1201 aatatggcgt caggcttcat catcagaaag ggggcctgac gcctgaaaaa gtgaacaaca
     1261 gacagtgttc ggattatcaa tattcagttt ttaacgttga actggattca ttactgcgtt
     1321 gtttaagaat ttcccgcgcc gaatctgcca ttatcatgac agcacctgcc agcatcagag
     1381 tatctttcaa taccaagcga ccagcaccgg ataaataagg gaaaccatga tgagcgtcac
     1441 ccaatgcggg tacccatgcc tccggggtgg tgattaaaaa tgagagtgtt accagcggcg
     1501 tggtatatgc catcagtcct cccaataaac ctaaccagcg attgacagga ttagccaaaa
     1561 ccagcagagc aataatcacc tccacgacgc caagaccgtt ggaaaaacca taggtattat
     1621 tggccgattg ccatgccctt gcttctggtt tgtattcgcc ttcgtgagtc agatactgtt
     1681 tatagtcttc cgggtgttca taaaagaacg acattagtgg actgtttgcg acgaatggtg
     1741 taatgctgtc tgcctcgtaa gggacaaact ttaataaccc aatccacata aaaacaatcg
     1801 caatactcag acgaatcaat gacaggccaa ttttatctcc ccgacttaac aggtgcaggt
     1861 atttttccat tgtgaacatc cttatttata caatactgct gttgcggttg aattattatc
     1921 aattcgcatc gatgtgactt tccaggatga agcattatat tttctggcta tatcagcaat
     1981 ggtatcctcc agaactgtcg gtgatgctga tactgacgag acgctgatat gtttcagaat
     2041 tattttatca tctgcattgg tggaaaattc cataacgcca gataatagtg ttgcaaataa
     2101 aacagatttt ttaaacatat aaaataccat caatgaagtg attaagaagt tatagagtaa
     2161 cagagaatta ataagattct tttctctgag acgccagaat atttgttctg gcgtctgatt
     2221 ttgagtttat ttgactaatg aaaatagatc attgagtgat tcgctcatcg acggatgagt
     2281 aaatatctga tcgcgtaata tgctataagg cagcccggca tccatcacca ttttcactat
     2341 attgatcatc tcgtgggagt caacacacag cagtgatgcc cctaatatac gttgggtttt
     2401 attatcaaca atcgctttta ataccccacg agtatcattc atcactctgg cacgcggaat
     2461 tgcagctaca ggcaatgtca ccacctgaat atcagcacca ctctctctgg cctgttcttc
     2521 tgtcatacca accctggaca gtggcggtgt catgaatacg gaataaggca catttttccg
     2581 atcatcagta ctacgtctgc cttctcccag caactcatca cgcacaatgc ggtaatcatc
     2641 cagtgaaata taagtaaatt gcagcccgcc ggtaacatct cccatcgccc aaatattgtc
     2701 tgcggtggta tgtaattgct tgtcaacgac aattgccccg cgctcgttta ctgcgatacc
     2761 ggcattttct ggatgtaacg aagcggtagc cggttgacga ccggaagcta ttaacagtgc
     2821 atccaccgcc agttgggcgt gctcgctatg cacttgcact tgattttcat ggtgactgat
     2881 tcgctccaca tgggcattga ggataatatc gacgccctga tcgcgtaaaa tcgtcgcgat
     2941 attatcagca atatcccgat cttcccgagg caaaaacagc gaagctgctt ctaaaatggt
     3001 taccttgctg ccaaaattag cgaacataga ggcgaactca acgccaatat atccgccgcc
     3061 caaaatacct aaatgcccag gcaattcttt tagattaagt aatccggtgc tgtcatatac
     3121 accaggcgtg gtggtaattc caggaattgg cggaaccacg gtttgtgcac cggtattaat
     3181 aaaaattttc tcgccatgaa tttccagatt tccctcaggc cgatgaacac gcaggctatg
     3241 attattgata aactccgcct ggccgtcgat cacgtcgata ttgggcatat ccgcaagatt
     3301 atgaaaattc ttattacgta aaaaattaac cacttcattt ttacgctgta tggcacggac
     3361 aaaatctgtg tgctgctgtg cgtcatgaac caatgttttg gttgggatgc agccgatatt
     3421 aatacaggtc ccgccataca ttgcatttga ttgttcgatg agagccactc gccaacctgc
     3481 ttttgccagc gtgacggcta atgtttttcc agccttgcca aaaccaataa taactgcctg
     3541 atatttattc atgatgattt cctgtcgaaa cacgttcatt tgacatcatg actatagacg
     3601 caaaagagca aataccattt cttcacaacg cttgatattt tgtcttatcg tctggtattt
     3661 tctttttctt aatcagaatc acaaaacaaa aatcatgatt aaccatttga tggtattaga
     3721 ttagttattt actaagattg ttggtgtttg taatcaaaaa ccactcagga gtctgatatg
     3781 gatgccctta gccgtttgtt gatgcttaac gctccacaag gaacgatcga taagaattgc
     3841 gtgttaggaa gtgactggca gcttcaacat ggtgccgggg aattatcggt tattcgttgg
     3901 catgcgttaa cgcaaggagc ggcgaagctg gaaatgccga cgggagagat ttttacatta
     3961 cgcccgggaa atgtggtcct gctaccacaa aattcagctc atcgcctgag tcatgtcgat
     4021 aatgaatcga cctgtattgt ctgcggcact cttcggttgc aacattcggc gcgttatttt
     4081 ttaacgtctt tgccggaaac gctgttttta gcaccggtta accacagcgt ggaatataac
     4141 tggctgaggg aggcgatccc gtttttacaa caggaatcca gatcggcgat gccgggagtg
     4201 gatgcactgt gtagccagat ctgcgctaca ttctttaccc tcgcggtgcg tgagtggatt
     4261 gcacaggtta atacagagaa aaacattctc agtttgcttc tgcatccacg tcttggtgcg
     4321 gtaatacagc aaatgctgga aatgccagga cacgcctgga ccgtcgaatc gctggccagc
     4381 atcgcccaca tgtctcgggc aagttttgcc cagcttttcc gtgatgtttc cggaaccacg
     4441 ccgctggctg tattaacaaa gttgcgtcta caaatagcgg cccagatgtt ttcccgggaa
     4501 atgctccctg ttgtggtgat cgctgagtca gtaggctatg ccagtgaatc atcttttcac
     4561 aaggcgtttg tccgcgagtt tggttgtact ccgggagaat atcgggaaag ggtcagacag
     4621 cttgcaccct gaataaaacc gccagaaatc agggcaaaga taatccgcat tccgggagtt
     4681 gtgagccttt caactatttc tatttccaac ggtggtttgg g
//
LOCUS       DAZPEP010000237         4612 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZPEP010000237 DAZPEP010000000
VERSION     DAZPEP010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.239"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(1..1133)
                     /locus_tag="ACHUT9_004117"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="ACHUT9_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUT9_004118"
     CDS             complement(1133..1315)
                     /gene="ymfR"
                     /locus_tag="ACHUT9_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFR1350456.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="ACHUT9_004119"
     CDS             complement(1327..3060)
                     /locus_tag="ACHUT9_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFR1350457.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="ACHUT9_004120"
     CDS             complement(3057..3551)
                     /locus_tag="ACHUT9_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFR1350458.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="ACHUT9_004121"
     CDS             complement(3673..4023)
                     /locus_tag="ACHUT9_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1350459.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="ACHUT9_004122"
     CDS             complement(4066..4530)
                     /locus_tag="ACHUT9_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1350460.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..>4612)
                     /locus_tag="ACHUT9_004123"
     CDS             complement(4527..>4612)
                     /locus_tag="ACHUT9_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1350461.1"
                     /translation="TYAGGKQWKGLMTRREIEREVCLWGQQ"
BASE COUNT          996 a         1369 c         1155 g         1092 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt cc
//
LOCUS       DAZPEP010000238         4519 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZPEP010000238 DAZPEP010000000
VERSION     DAZPEP010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.240"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..264)
                     /locus_tag="ACHUT9_004124"
     CDS             complement(<1..264)
                     /locus_tag="ACHUT9_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1350462.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAP"
     gene            493..1101
                     /locus_tag="ACHUT9_004125"
     CDS             493..1101
                     /locus_tag="ACHUT9_004125"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFR1350463.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1199..2590
                     /gene="selA"
                     /locus_tag="ACHUT9_004126"
     CDS             1199..2590
                     /gene="selA"
                     /locus_tag="ACHUT9_004126"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFR1350464.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEIIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2587..4431
                     /gene="selB"
                     /locus_tag="ACHUT9_004127"
     CDS             2587..4431
                     /gene="selB"
                     /locus_tag="ACHUT9_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFR1350465.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT         1018 a         1142 c         1330 g         1029 t
ORIGIN      
        1 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
       61 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac
      121 cggatggccg gacgtcactc cgccggggca caccgaacag gccacaccgg tgggggcacc
      181 gatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg
      241 acgcgctgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct
      301 tccaggcaaa accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa
      361 acaattagga aaatatttat atcacaatga taaatatgtg atttatttgg aagtaacaaa
      421 ccatcaacat tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac
      481 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
      541 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
      601 gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag
      661 ggcgaatgct ggtttgattc gccgatcatc gctgaatata ttgagttaat gaatgtcgct
      721 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcgctg
      781 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccggcggcg
      841 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
      901 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
      961 gccattgcct gtgctgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     1021 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc
     1081 accgaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     1141 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     1201 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     1261 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     1321 tcagatgctc gacgaagcgc gagaagtgat tcgtgacagc cagacgcttc ctgcgtggtg
     1381 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaggcgcaga gcgcgctgcg
     1441 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttggacgag ctttacaggc
     1501 ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     1561 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcaactgc tgtgccgtat
     1621 tacgggggcg gaagatgcct gtatcgtcaa taacaatgcg gcggcggtgt tattgatgtt
     1681 ggcggccact gccagcggga aagaggtggt agtatctcgc ggcgaattgg tggagattgg
     1741 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg
     1801 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     1861 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga
     1921 agcggaactg gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag
     1981 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     2041 gattgcggcg ggcgtcagtc tggtgagctt ctccggcgac aagttgttag gcgggccgca
     2101 ggcagggatt atcgttggta aaaaagagat aatcgcccgc ctgcaaagcc acccgctgaa
     2161 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     2221 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     2281 agaggtcatt caaatccagg cacaacgttt acaggctccc cttgccgcac attacggcgc
     2341 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     2401 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     2461 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg atgattggtc gtatttatga
     2521 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     2581 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     2641 gtgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     2701 gatctcggct atgcctactg gccgcagccg gatggtcgcg tgcctagttt tatcgacgta
     2761 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     2821 ctgttggtgg tggcgtgcga tgacggcgtg atggcgcaga cccgtgagca tctggcgatt
     2881 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     2941 gaagcgcgtg ttgatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt
     3001 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     3061 gagcatctgc ttcagttacc aggacgtgaa cacgccagcc aacatagtgt ccgcctcgcg
     3121 atcgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     3181 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     3241 cgtgcgctgc atgcacaaaa ccagccaaca gaaaccgccc atgccgggca gcgtatcgcg
     3301 cttaacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     3361 gatgcgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacgccgctg
     3421 acccagtggc agccgctgca tattcaccac gccgccagcc acgttacggg acgcgtttcg
     3481 ctgctggaag ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac
     3541 gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg
     3601 atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct
     3661 cttgcacggg cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt
     3721 aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg
     3781 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgcccgc
     3841 tggcagcgga aaattctcga cacattagcg acttatcatg agcaacatcg cgatgaacct
     3901 ggtcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctggta
     3961 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     4021 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     4081 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     4141 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     4201 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     4261 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     4321 ggccgaaagc tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     4381 cgtggaaatg atcatttatt gcgcgacgca ttattatttc cggaaaaata aggaaatgat
     4441 taattaattt ttaaaataaa ttaatacaaa tttctggtaa tgactccaac ttattgatag
     4501 tgttttatgt tcagataat
//
LOCUS       DAZPEP010000239         4490 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZPEP010000239 DAZPEP010000000
VERSION     DAZPEP010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.241"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..461
                     /locus_tag="ACHUT9_004128"
     CDS             <1..461
                     /locus_tag="ACHUT9_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFR1350466.1"
                     /translation="QNGLAKALREIGRIERTLFMLDWFRDPALRRRVQAGLNKGEARN
                     ALARAVFMHRLGEIRDRKPENQSYRASGLTLLTAAISLWNTVYMERAVDALKRKGMKI
                     NAQLLSHLSPLGWEHINLTGDYIWKNSRIPTSGKFRRLRSVKIDKVKKQP"
     gene            complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHUT9_004129"
     CDS             complement(987..2162)
                     /gene="senB"
                     /locus_tag="ACHUT9_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFR1350467.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(2231..>4490)
                     /locus_tag="ACHUT9_004130"
     CDS             complement(2231..>4490)
                     /locus_tag="ACHUT9_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFR1350468.1"
                     /translation="NVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVSV
                     GKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMSG
                     FGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEGI
                     NALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGSI
                     GVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSAR
                     TYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSAQ
                     NRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPFA
                     PSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDDK
                     EGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAETW
                     QAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDWE
                     GISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTYF
                     GAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQD
                     LPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSAR
                     GRTWIFGGDIRF"
BASE COUNT         1140 a         1050 c         1046 g         1254 t
ORIGIN      
        1 aacaaaatgg gttggctaaa gctctgagag agattggccg tatagaacga acactgttta
       61 tgctggactg gtttcgggat ccggcattgc ggcgacgtgt ccaggccggg ctgaacaaag
      121 gagaagcccg taatgctctg gcccgtgctg tattcatgca tcgattgggg gagattagag
      181 atcggaaacc ggaaaatcaa agttatcgcg ccagtggatt gactttattg acggcggcta
      241 tttccttatg gaacacagtt tacatggaaa gagcggttga tgctctgaag cgtaagggca
      301 tgaaaataaa tgctcaacta ttatcgcatt tatcaccatt aggttgggaa catatcaatc
      361 tgacaggcga ttatatctgg aaaaacagcc ggataccgac ttcaggtaag tttcgtcgtt
      421 tgagatcagt taagatcgac aaggtcaaaa aacaacctta acgtacaata attttcgata
      481 tccaaactga ccccaagtat aaaaaacagc ctgtcacggt ataaaaatcg accatgcaga
      541 aattatgctg gttattttac cgctgctggt ccctcattca gtgactgccc caggggtttt
      601 catgctctcc ttatagtaca ataatttcca tatccaaact cacccctact ttgactttta
      661 tcatagtcaa aacctcttaa cgtgagtttt cgttccactg agcgtcagcc ctctaaaact
      721 actgaataat cagttagaaa acaaacaatc ggaaaaatct ggggttccgg cctagaaccc
      781 catgaacagg catagctgtg atttcattta attattggca caaatcagtt gatcgtacaa
      841 tgctgtgcct gtctcgtttt ctggagattc ctcagcctca tggagcggcg taacaggcgt
      901 accggcagga cagagatgtt tgcttttcct agtccggcca gtttagtgga ataacggagt
      961 ttttatccca tgttttttcc ccccgatcac gactttttca aatcaatgct cagaatacgt
     1021 tctttagctc cttccacaaa cacatcagga tcccaatcgc ggttaaagcc ttctttgatg
     1081 ccaatgtaaa gggtatccat atcgtccgac aacaccatct ggtttgggta gaccggcatg
     1141 ttaaattcac gggtgatgct gtatttggtg gcatccacta cgctgatggt gccgctagtg
     1201 aagttggtga catacagttc gttatatttc ggattgtatt tcaccgcgtt agctccggct
     1261 cctgtgtgca catagccaag ctgtttaccg tcgcgtaagt caaaaatata gagtccatct
     1321 tttgtaccgg aacgatcgtc atggtcgaat accaccacaa aggcacggtc agaagcggag
     1381 tccaccgcca gattctgcac acttgtgatt tgtggtctcg gatctttcaa cgggatggta
     1441 cgcagcagcg tctgattttt cgcatcaatt tcgttgatgt aatctcttcc gccaacgtag
     1501 attttctgtg ttttttcatc catcgtcaga ccatacgctg tacctttgta agcatcctta
     1561 acctcgcctt ttaaggcaag cgtcgtcccg tcgagcatta acagcttatg cagaggcttc
     1621 atgccctcgg ccgttttcag catattgcta taactcacaa acagggtatc ggaatcctgg
     1681 ctgtaaacca tatggcgaaa atgctcaaaa cgcgaatctg cagcgtttgc cgctttaaaa
     1741 cttaacctgt cgctggttct taccagtttg ccggttgggg tgtcaaacat actgatacgc
     1801 agggacgctg actgggtgtg tccgatatag agctgatgtt tatcctgatt tatcgccagc
     1861 gaaaatgccc ggtatggcat cgtgtattta ttttcgactt tcagcgaatc tgaattaaac
     1921 gaatacagat agcctttatt ggcttcacga tttacacgat cggtgactgc tgcaaacacc
     1981 tttttactgg cattatcaaa cgccagttca tagacgcctt catcagggga ctgaaggtaa
     2041 ttcgttttca gggcaaatcg cgaaagttcg cgttcaggtg gatcgatagc catggccatc
     2101 gtgggtaaaa atagtgaaat taacaggtat agcggtgctt tggataaagt gaaaatattc
     2161 atttttcgtt cctgcaataa aattgacata agagagaaaa cgtattgtct gaacctagcg
     2221 gaaattgtca tcagaaacga atgtccccgc caaatatcca tgttctgcca cgcgcggaat
     2281 tggctgggtg ggtctcgtca ccaagacctg gcatcatgtt gagcttattc agcgcctccg
     2341 aataatctct gttcatcagg ttttgtaccg aaagtttcag tgttaggttg cggttgtact
     2401 cgtaagtacc atagagatcg ataatcgttg ggatctgcgg caaatcctgt ttgattattg
     2461 cgccggtatg ttcgtcctgc tcaaaatcag gcgacaggcg acgagccttg cctgtgtatt
     2521 ttatgatagt gcccagggtc aacgcgttat cgaagaagcg aacaccagta tccagcgtca
     2581 tgtattttct gggcagttcg gtcatatccc ctgcgccaaa gtaggtgctg gcgatggagg
     2641 ttggctggtc tgtttgctgc tggctgaaag agagtcggcc aaaagcaaaa cctgcatcat
     2701 aatccatctc cagttcgaag ccctttgcga taacgtcgct tgccgagtta acgtagatgt
     2761 acatattgtc gctgtattca tcgctaatgc cctcccagtc attgccaatc acctcaggca
     2821 tactgcattt acgacctcca gaacaaacca gataagactc gctgtagatg tagttctgga
     2881 tcctgctgcg gtacgccaga gccttaaagc gcagggcatc ctgttcgacc agtaaatctc
     2941 tggtatcaat gttaaaaccc gcctgccagg tttctgcacg ttcaggcttc aggaatgggt
     3001 tcatggatgc gcctcctgaa ttagagaaga acatctcctg gatgttcggg gcgcgcatgg
     3061 atttgctgta gccaatgaac ggctgaagcc atggtgttac ctgagcagaa agctgaactg
     3121 aagggttgaa gccaccctct ttatcgtcaa tatcgtagct accctgtggc acgcagataa
     3181 cgcgtgaatc gcaggcgggc ttgtaccctg tgatcctgtt acgtgtgtag ttgagattga
     3241 aatctgcctc ccagatgccg cgcgtaacct tcagcccggt atacagcgct gaaatatcct
     3301 gctggccgga gggtgcgaac ggattgttct cgatcgattc ctgattcgct ttcgggtcgc
     3361 ccgccgctga gtgaatggtc cggtcatagc gggtacgcat cagtttgctg cccagcataa
     3421 actccaggtc attgtccgcg acagtgaacc ggctggtatt gttgatatcc agcgcgtcag
     3481 aacgattttg cgctgaggtt ttgtagaaag tatacagcga gccatcacga tacttttgat
     3541 taccgcgact ggtactggcc gttacgttaa agtcaattaa ttcagaaaaa ggggtgtaat
     3601 gatatttaat gtaatagtcg tcactggtta tatcacgacg tgtaaattta ttttcatagg
     3661 ttcgagcgga aagttcgaag ctgttaaatt cgtctggtct gatatccatt ttatacagtt
     3721 gggatttggg gttctgcttc atatatttat cataaccaaa ctctttgctg ttaattccag
     3781 aaccatttga gaaattagag tacacagaac tgccgctcac agcagccata acgccaatgc
     3841 ttcccgtatc cgtaaaagcg tcgctttttc caccaagggc gatcattccg ctgcgtccca
     3901 gcccattact accgacagag aaacgtgaac gaaggccata tgtattaccg ttaaatatta
     3961 catcgtcaac gccaatagta cgcatatttg cactaccggc aagggcgttg atcccttcag
     4021 agccactgct gtcaccgcgt gtaacatcaa ctgctaccag taagttagga tctataagta
     4081 cgccagccat attgttagtt gaaccatgcg tcgttgttcc ggaggtagag gttccgtaaa
     4141 aactctgggt aataccatcg accatagtgt ttacacgacc aaatccgctc atgcctcgaa
     4201 tattcacact gattgctccc tgaccaggat ctatttgagt ataagtacca ggcatactac
     4261 gcactgttgc gtcaagtgat tgcaggtttt tgtccgttgt ccgggaactg gtcgcgcctg
     4321 ttttctcgag agcttcctgc tcactggtcg tcttcccgac agaaacgctg agcggactga
     4381 attctgccgc tccttttttg tcggaattag ttttttcact gatggattgt gagtaagcac
     4441 cgcagctgag caataatatg cctgagccga tagccagttt tataacgttc
//
LOCUS       DAZPEP010000240         4480 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZPEP010000240 DAZPEP010000000
VERSION     DAZPEP010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.242"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(7..1068)
                     /locus_tag="ACHUT9_004131"
     CDS             complement(7..1068)
                     /locus_tag="ACHUT9_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFR1350469.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="ACHUT9_004132"
     CDS             complement(1065..2462)
                     /locus_tag="ACHUT9_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFR1350470.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="ACHUT9_004133"
     CDS             2617..3615
                     /locus_tag="ACHUT9_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1350471.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4480)
                     /gene="ompG"
                     /locus_tag="ACHUT9_004134"
     CDS             complement(3726..>4480)
                     /gene="ompG"
                     /locus_tag="ACHUT9_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFR1350472.1"
                     /translation="EPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVH
                     YQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWL
                     SMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFST
                     QEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGL
                     SVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1227 c         1099 g         1062 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaatggattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg
//
LOCUS       DAZPEP010000241         4409 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZPEP010000241 DAZPEP010000000
VERSION     DAZPEP010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.243"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..105)
                     /locus_tag="ACHUT9_004135"
     CDS             complement(<1..105)
                     /locus_tag="ACHUT9_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350473.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNV"
     gene            complement(173..2122)
                     /locus_tag="ACHUT9_004136"
     CDS             complement(173..2122)
                     /locus_tag="ACHUT9_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1350474.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="ACHUT9_004137"
     CDS             complement(2207..2542)
                     /locus_tag="ACHUT9_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFR1350475.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="ACHUT9_004138"
     CDS             complement(2542..2898)
                     /locus_tag="ACHUT9_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFR1350476.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="ACHUT9_004139"
     CDS             complement(2898..4394)
                     /locus_tag="ACHUT9_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFR1350477.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1021 a         1313 c          970 g         1105 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agacatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagcctta
//
LOCUS       DAZPEP010000242         4395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZPEP010000242 DAZPEP010000000
VERSION     DAZPEP010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.244"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..590)
                     /locus_tag="ACHUT9_004140"
     CDS             complement(<1..590)
                     /locus_tag="ACHUT9_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1350478.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="ACHUT9_004141"
     CDS             complement(583..993)
                     /locus_tag="ACHUT9_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFR1350479.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="ACHUT9_004142"
     CDS             complement(999..1532)
                     /locus_tag="ACHUT9_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFR1350480.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2586)
                     /locus_tag="ACHUT9_004143"
     CDS             complement(1510..2586)
                     /locus_tag="ACHUT9_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFR1350481.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLGENILSCDTERSIRERFSEYQVSGQRPGNDDD
                     FGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETTYT
                     VQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPADAY
                     LPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2583..3974)
                     /locus_tag="ACHUT9_004144"
     CDS             complement(2583..3974)
                     /locus_tag="ACHUT9_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization protein"
                     /protein_id="HFR1350482.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRESLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4021..>4395)
                     /locus_tag="ACHUT9_004145"
     CDS             complement(4021..>4395)
                     /locus_tag="ACHUT9_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350483.1"
                     /translation="TSADNDVDLDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIG
                     VFDFANNASPATMTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVN
                     VNGESGVTYENCKLNMKDINDD"
BASE COUNT         1052 a         1282 c         1039 g         1022 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgtcgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcgc
     2041 cgtcgccgct ttacctgttc cgggttttcc cagaaccagt cgcccgcatt catcgtcata
     2101 agccagcgtc tgaacctgcc ccagtaaccg gttaagacaa tcggcaacgg tttcgccgtg
     2161 ttccggctgg gcatcaatca ccgctgtctg cggcacgcca gcatcaacaa cggtgatgcc
     2221 aaatggcgca gccagttcac tgactattct gagcaggttt tttccgctct gctggagtgg
     2281 caaagcagag cagtcaacca gatcggctgt tttgcttcgc ccgacaattc ccatgctgac
     2341 gctgctggcg tcataacgaa gcggtagtgc ctccacatat ccggtgagca caggctcatc
     2401 cccgataagc acttcaacca gctcaccatt ttttatccgg ggttgataat cccggcttcc
     2461 gggccagcgg gtggtaatgg caacattaaa atcccgggca atacggttaa tgcccgcact
     2521 gatacggacg gatgtccagc cgccccattc gcgaccggaa acccgaagta aaacggtatc
     2581 attcatctga cgggtaccct taatgccctg accggaacaa agcccggatg agagatggca
     2641 tttcgatcca ggatatcagt ttcacgggac gcatcgtcgt accatgacgc agccagaaca
     2701 agcgcaggca gaacttccgc tggcgttcgc tctgcggttt cctccgtctg aaccagacgc
     2761 gcctgaatat cccggttcag ctctgtacgt aatgacgtca gctgaaaaaa cagcctgtca
     2821 tccgccgtac gcctgagctc ctgttcaatc gccgcgttca gcgattcgcg tatgatggtg
     2881 agattttccc gcgtgggtgg ttgcgctctc tcatcctgtc ctgtgctggc tgccacgctg
     2941 tcaagtgccg gatgagaaac gtgaataata tcggactgac gttcagtgga accaccaaca
     3001 gccacaaccg cctgctgatt tttcaccagg cttccgggtt gtggcagtga agtgaccgcc
     3061 cttgtggctt cgctgattgc cgtcgtccgg atgacagcag ccacaaggtt tgtctgctgt
     3121 ttttgcctga taaccgatgc ggaatctgtg ggccacaccg cacgcggtgc cagtcctggg
     3181 tccagcgtaa tgccggacat tgtcgttatg gactgcacca gatcctgtgt gttatccacc
     3241 agccgggttc cggcccgcca ggtatcctgc aacgtatgca caaaatcact ggcaaccgac
     3301 ggcggcatca gaatgacgga taaatcgccc tgcagtagtc tcgctccggc agaaacagcc
     3361 gaattaacca ttttgaaagc tgtctgaacg gttcccagca tatcggtcgt ccgggcaata
     3421 acatcgttct gaataaagtc cggtattccc gcgagatcaa aatcgccgaa catgtcttca
     3481 atcaactcat ccaggaatac ggacgactcc tccagttttc tggcggttgc agctccggcg
     3541 accggaaatg acagttcccc gctctcaaca aactgaaatg aaacccggca catacggcct
     3601 tcagtgccgg agtgagaaac ggtcacctgc ccgtcaatac agccctgcat ttcaccgaac
     3661 tgcggatgga tcagcgtccc cggcccggcg gtttcaatcg ctgtaatcag cctgtcgcgc
     3721 tgctctgcgt aatcatcacc aacgagatac gcattaatcg tcagccgtcg cgtggcacgc
     3781 ccgagatctt ccgtgtacgg tttgtcacgg tttgggtatt catggacctg aacgcggcgt
     3841 ccgaacgaac cctcgtcgct ctccaccgaa aacggaacgc cacgaaatga agcgtcgtaa
     3901 agattatcgc gccaattgat acctgaagat gcagaaaaga gtgaagaaag agaaggtaaa
     3961 gaaggtaagt ccatactgtc tccactataa aaaaaccgcc aatttctggc ggcttatata
     4021 ttaatcatca ttaatatctt tcatattcaa tttgcaattt tcgtaggtca ctcccgactc
     4081 gccattaaca ttaactccag ctctgatatt cccgttttct tttacatcta caaaaaactg
     4141 gcctcttggc atcttgaacg caaatgtaaa accaacagta tctgaatcaa aatcatcatc
     4201 tttatatatt gggatcaaaa atgtcatcgt cgctggtgat gcattgtttg caaaatcaaa
     4261 tacacctata cttttgccga attcataaga agcttttctc aatataatcg caccgctggc
     4321 agaaggggat gggcacgata tcctcagttg tctgaatcca tctgtatcca agtcaacgtc
     4381 attatctgca gatgt
//
LOCUS       DAZPEP010000243         4385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZPEP010000243 DAZPEP010000000
VERSION     DAZPEP010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.245"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..3511)
                     /locus_tag="ACHUT9_004146"
     CDS             complement(<1..3511)
                     /locus_tag="ACHUT9_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFR1350484.1"
                     /translation="MDENALGFTSYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQ"
     gene            complement(3963..>4385)
                     /locus_tag="ACHUT9_004147"
     CDS             complement(3963..>4385)
                     /locus_tag="ACHUT9_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1350485.1"
                     /translation="ESANYIGARSVTAAIKPDIKLQPTRHVKTMDALQTGCVHINTVN
                     KALRVMGYDTTQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKA
                     EHLEERRLMLQWWADFLDANRDKGISPFEYAKINNPLK"
BASE COUNT         1124 a         1126 c          906 g         1229 t
ORIGIN      
        1 cctgacgcct ctcaagacta ttataaatag cctcaatctc acccttcagg ttatcaccgc
       61 aataaaccat tgatgtatcg tatcgttcat atcgcgcttc ccgtgtcgca ctaaaccagt
      121 tgaacgatcc cacacacagc aaaccatcat caccaataac aattttgcta tgaacacggt
      181 ttaccagctt tgtagcaata cccagcgcat tcagtttctc cagcgccgct ttaaagttct
      241 gctgcttctc ttttcgcttc tcaaaatcat tatgttcagt gttgtagctt ctgtcagtga
      301 ctatcgtgac gttaattcca cgtgaacacg ccgcaatcat ggaatcaaga aaaccggttt
      361 gctccagcct ttgccaggtc agccatggag aaattatcgt gatgtgttta ctggtatttt
      421 caaatgtctg attcaggaaa ttatcatgtt gctccacacc atgaagtgtg tagattttgg
      481 tcccggcggt ttttaaatcc ttacgctctt tataatcaaa agagagcgca ttcttctctg
      541 actcaaagag gtattttgcc agtaatcccc gtggcgatga ggctggctgg acctcaaaca
      601 ggtccatatc gccgaagacc agaaaactgt ccttcgcacg ggagactgca acattcagca
      661 tgctgttatc gctatcaata aacccgccgt cttcatgttt tgaatagact ggcgagaata
      721 tcacaatcgc tctttccgct ccctgaagag agtgcacggt gcccactgtg agcgactttt
      781 cattcgtgcc tgtactgata ccttgtttgc ccagcacctg tttgatagtg gatacctgag
      841 cgctaaaagg cgtcacaata ccgacaactt catgaagcga tttaccgtaa tgcgcttcaa
      901 tattttgctg gttctctgcc aaccagaccg ctatcgtttc agcctcaagc aaattatatc
      961 gacttccact acttgccagc tctcctttac catcaatatg gagatacccc attgcgggca
     1021 ttaaattgct ctcttcacgc cctcttttag gcaacaactt accgtgatag cagagcgtat
     1081 tacagtatcc aataatattg tcgtagcacc ggcggtgttc atataggtac ataccacgag
     1141 ccagttcggg atcatattga tagcgcgaag caaactgcgc tattttcata acgctgccag
     1201 atgcggcact tttaccaagg tctgcgattg ccgtatattt ctcggtaatc tcttcttgcg
     1261 tactgccaga cagaattttt tccgccagca tgttaccgac atcaatcgca ggagcaatac
     1321 tccatattgg cgggatctgc tccgtatcgc caatcactaa tgccttctta gctaatgcaa
     1381 acgaggcagc agccacttca ggaagcacct gcccggcttc atcgacaatg agtaaatcgg
     1441 caaaatcata caaataactt ttctcgaatt tacgttgtcc tttgtgctca cttatctgca
     1501 tattaccggg cagcatatag catgtcatta ccacacatgg cgtgagtttc attcgacgtt
     1561 gccaacgggc ggttacccct ttagcacctt ttttcttctt ctcgtcctgc agatcatcaa
     1621 tgctggccat atccatcagc caacgacctt cccagtagtg agtcgtcagt aaaaatgcag
     1681 ggaagcgaat ctgcgtatca gccagttcat cggcctgtga gaagctcagt tcctcgtcgc
     1741 cctcataccc taaatcaaat gccagcctct gccactcctg aaccgcctgc tgttctttaa
     1801 gaacgatttc atgggcggag tcaatctgct gccggtatgt tgtttgctcg cgctcagcgg
     1861 aattgagcag cccatcaata ttacgttcga tagtttcagg atcagaccac tgatttcctg
     1921 caatcagcgc gcctaattta tcttcgagaa acagctgtat ttggtactgt cgcttattgc
     1981 gaaccgccgg gagccaggaa aataatgaat agatcagtga ttcaccggcg cgatattttt
     2041 tccattccgt tttagcactc ttcagtagag tgactttttg ttcttgtccg gaaagtaatt
     2101 tatttaaatt atcgagatat tgctcaatat cattagcaat aagctcacgc gcagcccgaa
     2161 tctggcttaa cgtttgccat gttgcgttca gtcttatcag ttgctcagat tttgctgcca
     2221 actgaccatg caggagttca atgacctttt caggggatga acactctttt ccaggaaagg
     2281 ctgccttagc cttttccaga taaaacagca gtgcatcctc tacatactct tttgactcaa
     2341 cctggttgaa gaaatcttca gtttgatatt ttttggctgc ctcagcttta cgactgcttg
     2401 agggaaaata agcaccgaag cttttcagct ctggcaacca tcgcccggcc atcgcacctg
     2461 aaccttgcga aaagtctttc ccgaatgcct caataatgtt cgttacagcc tggttattcg
     2521 ttgaagtcgc gataataacc ggaggctcag atttttcgag agccgctctg gcccactgcg
     2581 tggcgatgat agaaagcacc agcgtggttt ttccggttcc cggagggcca ttaacagcaa
     2641 ggatatcgcc atgtcttgca tccagaaaat ggcttaaggc atcgcgctga gcctttgcca
     2701 gcggaaactt atctccggag tgtccaagcc tgtcgctgaa ttttgctcct ggggccagca
     2761 aagactctgc agcatgaacc tctcgcgagg cgaagcgatt gaagagcggc acatccttat
     2821 tgcaaacaat caggtgatca taaagagaaa ggatatggga actggcaccg ccagattgag
     2881 ccgttttaac aatataaccg tgctcagcga gttcatattg ctcaggtttt tcaatccagt
     2941 cgccggcaac gctcttcagt agcctctctg agtcatacag atattgacgc cactcctgct
     3001 gcaaggcggc atatcgtgct tcccgttctt catccgtttc ggcagtctta tcaacgctgt
     3061 catcaaagtt gatagagaac gtggtatggg tcgtcttgta tttgtcatac tgcccaatct
     3121 caccaatcga aaatgctcct tttggcaaag gttcaagcag gtctctggga atggaggtcg
     3181 ctggcgtcgg atataaaaaa ccctcacggc ttagcaaggc tggcgtcact atcggggtaa
     3241 caatatcagg cgcacctgca gaacggtcct taccatgctg cagtaaccgg aaataaactt
     3301 ttgggcgcaa gatgacatcg accgtttcga catcgtcttt ttctccctta aaaaatttac
     3361 tgacaatcgc ttcgtcaaga cgtcccgccg ctatcccatg ccagtgagtg aaatttttgg
     3421 cgtctttccg ttcaaaactg ccctttcctg actcagcatc cgcaagcgag ttgcgccagt
     3481 atgaggtaaa ccctaaagca ttttcatcca ttaccagtcc tgtgactcaa tgaaatccat
     3541 tgatttatcg aatataaaac caccatatgg acaactattg tccatattga aaaagttgga
     3601 tgcaatgttt tgtttataat tcggtatcaa atgtactggt tgtacaaggt tcagaacaca
     3661 agcacattca acagtacgtc aggactattg atttcataac agtgaataca ttttttaccg
     3721 tttataatga ctgcctgtgt tggacaagga taccagaaca gccgaaaaca ccgggggtta
     3781 ttacctgctc tcatggaaac cagatattcc tggtgctaat cacagtcata aatagcctga
     3841 agaacatgac gaagggactt taatggaagc cggagtaact tgccagaaca tcaatcttcg
     3901 tcatcattta caaagaaaaa taatcagtta aagcagtccg ggcaatcagc ccggactgct
     3961 tactatttta atggattgtt aatctttgca tattcaaacg ggctgatacc cttatctctg
     4021 ttcgcatcca gaaaatctgc ccaccactgt agcatcagtc gacgttcttc cagatgttct
     4081 gctttatgga tatacgcagc acgtacacca ttacgctcct gatggctcat ctgacgttcc
     4141 acagcatcgc gggaccacaa acctgattca atcaatgcac tgcacgccat cgcccggaat
     4201 ccatggccac agacatcctg ggttgtgtca taccccatca ccctcagggc tttatttaca
     4261 gtatttatat gaacgcatcc cgtttgcaag gcatccattg ttttgacatg acgggtaggt
     4321 tgcagcttta tatccggctt tattgcagcc gtaacgctgc gagcccctat gtaattcgcc
     4381 gactc
//
LOCUS       DAZPEP010000244         4364 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZPEP010000244 DAZPEP010000000
VERSION     DAZPEP010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.246"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..215)
                     /locus_tag="ACHUT9_004148"
     CDS             complement(<1..215)
                     /locus_tag="ACHUT9_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1350486.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="ACHUT9_004149"
     tRNA            complement(330..405)
                     /locus_tag="ACHUT9_004149"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="ACHUT9_004150"
     tRNA            complement(412..486)
                     /locus_tag="ACHUT9_004150"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="ACHUT9_004151"
     tRNA            complement(603..687)
                     /locus_tag="ACHUT9_004151"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="ACHUT9_004152"
     tRNA            complement(696..771)
                     /locus_tag="ACHUT9_004152"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUT9_004153"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="ACHUT9_004153"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFR1350487.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUT9_004154"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="ACHUT9_004154"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFR1350488.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUT9_004155"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="ACHUT9_004155"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFR1350489.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
     gene            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHUT9_004156"
     rRNA            complement(4237..4352)
                     /gene="rrf"
                     /locus_tag="ACHUT9_004156"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         1112 a         1095 c          983 g         1174 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aacgcaaaaa
     4201 ggccatccgt caggatggcc ttctgcttaa tttgatgcct ggcagttccc tactctcgca
     4261 tggggagacc ccacactacc atcggcgcta cggcgtttca cttctgagtt cggcatgggg
     4321 tcaggtggga ccaccgcgct acggccgcca ggcaaattct gttt
//
LOCUS       DAZPEP010000245         4329 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZPEP010000245 DAZPEP010000000
VERSION     DAZPEP010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.247"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..582)
                     /locus_tag="ACHUT9_004157"
     CDS             complement(<1..582)
                     /locus_tag="ACHUT9_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFR1350490.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPNF"
     gene            complement(645..1094)
                     /locus_tag="ACHUT9_004158"
     CDS             complement(645..1094)
                     /locus_tag="ACHUT9_004158"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFR1350491.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="ACHUT9_004159"
                     /pseudo
     CDS             complement(1091..4183)
                     /gene="ebgA"
                     /locus_tag="ACHUT9_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1028 a         1090 c         1168 g         1043 t
ORIGIN      
        1 gaagttcggg atcatcgcgt caacggtgat ggcgtcagca ggctgtacgc cgccaaccag
       61 cgccgtacct gccagtaaaa tgtaggagag cgttaacagc agcatcagcg ttgaagtgac
      121 ggaggtaatt ggccccaaca ttttcgcccc gttggtggaa acccaggtgg agaaggcgaa
      181 cagcaccata ctgataatgg tggtggcaac cggcgtcata atatattcgt atccgaggaa
      241 ggcatacgaa gcataagcaa taacgcgcgg caacagtgag gtgaaaaaga acaggtttac
      301 gaaccagtag gtataggcag taataaatgc ccaacgtccg cccagcgaac tttttaccca
      361 tgcgtagaca ccggcttctg agtttttatt taacgaaaca aattctgcga tgatcagaca
      421 gaagggaata aaataaaaaa tcgtcgcgag gaaaaacatc ggtgccgagg ccagtccaag
      481 atcaatatta ttattgataa cgttgttaaa gctgtaaacg gcggcaaaag tcagcgagag
      541 caagccgaat ttgccgattg tattacgttt ggtatcagac ataacacatc tccgaaaatg
      601 agtagggtag cggtggcggc aaactccttt tgccgccgta gttgttattt gttatggaaa
      661 taaccatctt cgatggtgac tttgagaacc acttttttga ccgcgttatt gcagataaac
      721 cgatacgctt catggatatc gcaaataacg atttgccctt cgtggacctc aacggtttct
      781 ccgcagcctt ttaaatattc acggtcagtt tcatcacgat aatattccac tacctgtaat
      841 gtctctttcg gcgcatattc aattttttgc tgcccttgca ggtagtaatg cacttcaaaa
      901 tagcgtcgat gcccggtaaa tagcgcatcg gtcgcggagt ctgtctccac gcggtaagtc
      961 aatgagtcac cgatggagtg ggcgacgcca ggctgaatgt tgtcgatatt ttcaatcgct
     1021 tcaacgcagc gttgccactt cttgccagag gcgtaaatct ggcggaactg ttctaagtta
     1081 tcgatgatcc tcattgctta ttctcgctgt gcaaattcgt ggaaaagaac cctgcgccga
     1141 actcatacga cgccaggctt tgcgcggtag cttctccgcc agaaaccggc agcaacgtaa
     1201 agccgtagct gaagtcacgg aaccagacgc gccaggagtc cagcacctcg ctgccccagg
     1261 agttggagcc gaggccaagc agctggtgat cgaggttcag ggtgatgtca tcactgcgct
     1321 gcagctcgtt acagtgctgg gcagcgtgga tgttttccta ggtatagtgc caggcgctga
     1381 agttaattgg gcgctgcgga accaccagca gaccgttacc gtggcggtta gtcagtgccg
     1441 tccagcggac atgctgacgg ttaccgttgt tctgcgggaa gggatagttc tcgaacatgg
     1501 catcgacggt gctgcgccag atatcgatga tgttagcctg ctggctgtcg gcgtagtttt
     1561 cgcccggtcc acgaccgtaa tacgccacct gatcgtattc gccgttaatt cccatggtga
     1621 aaccgatgca cggaatgatg tgcggatagt cgccgtaacg ctcgccggaa agcgccacgt
     1681 taacctggcc atcggcagcg atgcgccaga tgtaggtgca gcgcattccg aaatcaaaca
     1741 ccggcggggc aataaccgtg cggctgatga tcaacacttc accctcgctc tgttctacgg
     1801 caaagtcgcg cagatgttcc tgcatgatct gcaaatgatt cggctgccac aacccttcgt
     1861 actcctgctt gtggttgtcg atcatcggct tgaagaagtt gatctttggc tcgcgggtca
     1921 gcagcgactc gccatttacc tgccaggatg tcggtttgcc actcattttt gagaaggtga
     1981 tcgcgaagtt gtagccgcga acggtgcagc tcaaacgatc gtcttccagc gtcagcggac
     2041 gcgcattatt tggtgcgaaa ggcactggct gcgcggtgtt ttccttcagc gggaactgat
     2101 aagtggcgat agaatgcccg gcttcgctgt agcgggtgcg ggaatctttg gtcaccgtaa
     2161 tgttgaggaa cgcttcgcgg gcgtccagct gcggcagcgt gatctgcaag ggggcttcgc
     2221 tgttcggcgc aacgtcgcgc agtttaatct gctgcgtcgc gagcgtttca ccttcggcgc
     2281 gcacctctgc gtgcagggtg tagtcatcaa gcgtggtaaa ccacagttta ttttcgactt
     2341 tcaactcgcc gcgagtcaga tcccgcgcgt ggatttttac cggcgcgata acctgtttgt
     2401 actctttcag gcccggtccc ggcgtctgat cggaatagat caaaccatca agacagaagt
     2461 tatagttgtt gggatagtcg ccgtagtcgc cgccgaattt ataccagaca ttgccgtggt
     2521 cgtcctgtgc ctggatcccg tggtcgcacc actcccagac ataatgaccc tgaatgcaat
     2581 cgtgcttata gaagacgttc tggtactccg tcagcccgcc cggtccgttc cccatcgcat
     2641 gagcatattc acagatgatg cgcggcttcg gatgcgggta ttcaccaaac tcattcatca
     2701 gcggcacgcg ggtgtacatg gtggaaataa tatcgaccac ttcagcatcg cgatcttctt
     2761 cgtaatgcac cagtcgcgtg tcatccagcg ccttcgctgc gtggtacatc gcgcggatgt
     2821 tacagccata gccggattca ttgcccagcg accagatgat gatcgacgga tggtttttct
     2881 gcgcgtgaat atggcgaaca atgcgctcga cgtagacttt ttcccactgc ggatcgtcgg
     2941 taatacggct gatatcgccg acattagcaa agccgtgcga ttcgacgtcg gtttccgcca
     3001 tcacaaacag gccgtagata tcacacagtt cgtaaaaacg cggatcgttc gggtagtgag
     3061 cggtacgcac ggagttgatg ttgtgctgct tcatcaactg gagatctttc tcgacgcgat
     3121 ccattccaac ggcgcggcct ttgcgatgat cgttgtcgtg acggttgacg ccgtgcagca
     3181 tcacataacg gttattgatc cagaacagac cgtcgcgcac tttgatatca cggaagccaa
     3241 cgcgttgtgg taccacttcc agaacgttgc cgttggcgtc tttcagcgtc atgaccagat
     3301 ggtaaagata aggggattct gctgaccatt gctgcggctg ttcgacagta aaagcaaagc
     3361 tggcgctggt cagtttttca attgccaaat gatcaatggc gctgctgtgc accacgcgtt
     3421 cgccatcaaa cagggtatat tccagcgtcg tgacgacagg ggaggcggcg agattttcca
     3481 gcaccacttc gcaggaaagc gtggcatcgc aataggcttc gtcaaagtcg gtacgcacgg
     3541 tgaaatcgtt aatatgtgtt agttgttttc cgatcagata aacatcgcgg aagatccccg
     3601 ctgaccacca catatcctgg tcttccacgt aggtagagtc cgcccattgc atcacgcgca
     3661 cacacaacag gttgtcgccg gttttaacca tcgcgctgat gtcaaactct gcggtcaggc
     3721 gactgccctt gctgaaaccc acatactgac cgttaacgta gacttcaaaa taggtttcga
     3781 cgccgtcaaa tttaatcagc gtctgtttac cctgccagcc gtcgctgagg gtgaaaatac
     3841 gttgataggc accggttggg ttatcgctgg gaacaaacgg cacatcgatg gggaacggaa
     3901 aaccttcgtc ggtatattgc agtttgccgt gaccttccat ttgccacatg gcggggacgg
     3961 taatatgccc ccagtcagcc attaactcag aggtgaaggc ttcaggcact tgcagcgggt
     4021 ggtcaaaaaa gtggaaattc cactgaccgc ttaagggcag aaacaggctg ctggtttcgc
     4081 gggcaaaggt acgcgcttgc gcaacagaat catatgaaaa aaagtacgca cgtggcgcaa
     4141 gtcggttttc gtgggtgagc tgaatgtttt cccagcgatt catagggcct cccggtagag
     4201 agaaatcgga accattcatg gtgatggtag tttgtgtaat tgctggggag tttagtaaaa
     4261 tttttactaa aaagtggcag gtaaatgcat ttactttaac tggatcacga aaataaacgg
     4321 cagcactgt
//
LOCUS       DAZPEP010000246         4312 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZPEP010000246 DAZPEP010000000
VERSION     DAZPEP010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.248"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1873)
                     /gene="speC"
                     /locus_tag="ACHUT9_004160"
     CDS             complement(<1..1873)
                     /gene="speC"
                     /locus_tag="ACHUT9_004160"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFR1350492.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     "
     gene            complement(2036..2119)
                     /gene="yqgH"
                     /locus_tag="ACHUT9_004161"
     CDS             complement(2036..2119)
                     /gene="yqgH"
                     /locus_tag="ACHUT9_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFR1350493.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            2272..2979
                     /locus_tag="ACHUT9_004162"
     CDS             2272..2979
                     /locus_tag="ACHUT9_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFR1350494.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3085..3160
                     /locus_tag="ACHUT9_004163"
     tRNA            3085..3160
                     /locus_tag="ACHUT9_004163"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3118..3120,aa:Phe,seq:gaa)
     gene            3358..>4312
                     /locus_tag="ACHUT9_004164"
     CDS             3358..>4312
                     /locus_tag="ACHUT9_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1350495.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1115 a         1141 c         1037 g         1019 t
ORIGIN      
        1 tatgcgcatc ctgggggttc atcaccactg acgggaaact ctgctggcgg aacatcgctt
       61 tttgtaggtc tttgacgtcg aaactgacat acagatcgtg catctcctga cacaactggc
      121 gcagggtgta gtcgcgatag cgcaccggat atttgttata aacgctcggc aacacctcag
      181 ccagcggcga gtcatcctca atatgctgtt caaattgcgc cagcatcgcc accagttgtg
      241 ccagcttctc gtggctttcc gccggagtta ataagaacag aatggagttg agatcgcact
      301 tctccggcac aatgccgttc tcacgcagat agtgcgccag aatcgtcgcc ggaacgccaa
      361 agtcgctata ttcgccggtt tcggcattga tacctggtgt agtgagtaac agcttgcacg
      421 gatcaacaaa atactgatcc gcggcatatc cttcaaagcc gtgccacttc gcccccggct
      481 caaaactgaa aaaacggcgg tcgctggcta acacagatgt cggataatcc tgccacaatt
      541 tgccatcaac aacgggcggg ataaacgggc ggaacagctt acagcgcgca agaatagcct
      601 tgcgcgactc aatacccaac tcaacgcact cagcccacag ccgacgccca ctctcccctt
      661 catgaatttt ggcgttaaca tccagtgcag caaacagcgg atagaaaggg ctggtagaag
      721 catggagcat aaaggcgtta ttcaaccgct tatgcgggca aaaacgcgcc tgtccgcgga
      781 tatggttatc ttttttatgg atctgcgacg tctgtgagaa tcccgcctgc tgtttgtgca
      841 ccgactgagt cacaaagatc cccggatcgt tttcgttaag ttctaatagc agcggcgagc
      901 tatccgccat catcgggata aactgttcgt aaccgaccca cgcggaatca aacagaatgt
      961 aatcacacag atgcccaacg gtatcgatca cctgacgggc gttatagaca gttccgtcat
     1021 aggttcccag ctgaataatc gccaggcgaa acgggcgcgg cagctcggct ttttctggcg
     1081 caacgtcgcg aatttgctgg cgcagatact cttcattaaa acagtgcgca tcaataccgc
     1141 caatgaaacc aaacgggttg cgtgaagctt ccagatagac cgacgtcgcc cccgcctgaa
     1201 tcagcgcgcc gtgatgattc gacttatggt tgttacggtc gaagagcacc agatcgccac
     1261 gcgttaacag cgcattcgtc accactttat tcgctgccga tgtgccgttc agcacaaaat
     1321 aggttttatc ggcatgaaag actttggctg cgaatttctg cgcatccttc gccgatcctt
     1381 catgaataag cagatcgccc aattttacgt cagcgttaca catatcggcg cgaaagacgt
     1441 tctcaccaaa gaaatcgtaa aaatggcgtc cggcaggatg ctttttaaaa aacgcaccat
     1501 gttgatgtcc agggcaagca aaggtgctgt tgcccatctc aacgtactgc gtcagcgtgt
     1561 cataaaacgg tggcagcaaa ttctcttcat actgacaggc tgcggattcc agctccagcc
     1621 actgctgctc gttgccgttg attaccgccg taacgcccgc aggtaattca acagcatgtt
     1681 cggaatacaa aaacaccggt agatgaaaac cggtgcgctt aagcaacgca agaatgccac
     1741 tgcgactatc cgcagcggta atgacgactg ccgcgacatc cgtaaaatca gtatctccca
     1801 acgccaccac gcggcgatga gtagaaagtc gggataccag ttcactactg gcggcaatat
     1861 tcattgattt cataagcgca aaacccgttt cggggaagta agaataccgg acaaagtgta
     1921 aaaccctgcc ccatgagata tgggtcaaac tggtcaccag ctccgaccgc cagacatcag
     1981 taaaagcaga aacgctctga ttttactgtt gtcctgcagt gaacgtgcgt taacttcacc
     2041 gcatgagcag taacatagaa agggaaacgt ttcgcgcgaa acggcgataa gcgagagaat
     2101 gtaaggagat ggcgtgcatc gggcaaactc cgtacagaga ggagaaaatt cgcgcaatca
     2161 tggcaccttt cgctaaggcg tgcaagccag gactttgcga ccagacaagc catcaacaag
     2221 gcaaaaagtt cataataaga aaatcaaaca atacagctaa caggaactct tgtggtcatc
     2281 ggccctttta tcaacgctag tgcagtctta ctgggtggcg ttctcggcgc actgctcagc
     2341 caacgcttac cggaacgtat ccgcgtctcc atgacatcta tttttggtct ggcatcgctg
     2401 gggattggta ttttactggt tgtgaaatgc gccaaccttc cagcgatggt tttagctacg
     2461 ctacttggcg ctctaattgg cgaaatttgt ttgctggaga aaggtgtcaa tacagcagtc
     2521 gccaaagcac aaaatctgtt tcgtcactca cgtaagaagc cagcgcatga atcttttatt
     2581 cagaattatg tcgcgattat tgtcctgttt tgcgccagcg gcaccgggat cttcggggcg
     2641 atgaacgaag ggatgaccgg cgatccgagt attttaatcg ccaagtcatt tcttgatttc
     2701 tttacggcga tgatcttcgc ctgctcgctg ggtattgcgg tgtcggtaat tagtatccca
     2761 ttactgatca tccagctaac gctagcgtgg gctgccgcgc tgatattacc gctgaccaca
     2821 ccgtcgatga tggcagactt cagcgccgta ggcggtttat tgctgctggc aaccggatta
     2881 cgcatctgtg gcattaaaat gttcccggtg gtcaacatgc ttccagcact ccttctggca
     2941 atgccgcttt ccgccgcctg gaccgcctgg tttgcctgac aatgcgtgca atagcggcaa
     3001 agtgatgata gattgtgcag tctgcagtaa attgaagaaa tttgattgac gagacgaggc
     3061 gaatcaggtt taatgcgccc cgttgcccgg atagctcagt cggtagagca ggggattgaa
     3121 aatccccgtg tccttggttc gattccgagt ccgggcacca aattcatatc aacggacctc
     3181 cacggaggtc cgtttttcgt ttcagaacgc cacgatttaa gcgttctgcc tacaaatcaa
     3241 ttctaccgaa ctcaaccaga ttccccccac atcaacccca ttgtgtgggt ataattgcgg
     3301 gcatacccca gttcgacaga atttgtaccc tctttaacgc attcagaagg ctgaactatg
     3361 gcactgactg acgcaaaaat ccgggctgca aagcccactg acaaggctta taaactcact
     3421 gacggggctg gcatgttcct gctggtacat cctaatggct cccgttactg gcgtctccgt
     3481 tatcgtattc tgggtaagga gaagactctg gcacttggtg tgtatccaga agtttctctc
     3541 tccgaagctc gtacaaaacg ggatgaggcc cgaaaactga tttcggaggg ggttgaccct
     3601 tgcgaacaga aaagagctaa aaaagtagtc cctgatttac agctctcttt tgaacatatt
     3661 gcgcgacgct ggcatgccag taataaacaa tgggcacaat cacacagcaa taaagtactc
     3721 aaaagccttg agactcacgt tttccccttt atcggcaacc gggatatcac aacactcaat
     3781 accccggatc tgcttatccc tgttcgtgct gcagaagcta aacaaattta tgaaatcgcc
     3841 agtcgtctgc agcaaagaat atctgccgta atgcgttatg ccgtacagtc tggcatcatc
     3901 agatataatc ctgctctgga tatggctggc gcattgacta cggtaaaacg ccagcatcgc
     3961 cccgctcttg atctttctcg cctgcctgaa cttttgtcgc gtattggcag ttacaaaggg
     4021 caacctgtca cccagcttgc cgttatgctg aatttactgg tttttattcg ttccagtgaa
     4081 ctcagatacg cccgctggtc tgaaattgat attgacaatg ccatgtggac tattccagcc
     4141 gaacgcgaac ccctgccagg cgtaaaattc tcacaccggg gctccaagat gcgaacacca
     4201 catcttgtgc cactcagcaa acaggctgta gccatactga cagaactaca gacatgggct
     4261 ggtgaaaatg gtctgatctt tacgggagca catgacccgc gtaaaccaat ca
//
LOCUS       DAZPEP010000247         4307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZPEP010000247 DAZPEP010000000
VERSION     DAZPEP010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.249"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            181..333
                     /gene="hokA"
                     /locus_tag="ACHUT9_004165"
     CDS             181..333
                     /gene="hokA"
                     /locus_tag="ACHUT9_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFR1350496.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(310..381)
                     /locus_tag="ACHUT9_004166"
     CDS             complement(310..381)
                     /locus_tag="ACHUT9_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350497.1"
                     /translation="MRNAVRKAGITSRQRLLLFQIAG"
     gene            complement(521..733)
                     /gene="cspA"
                     /locus_tag="ACHUT9_004167"
     CDS             complement(521..733)
                     /gene="cspA"
                     /locus_tag="ACHUT9_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFR1350498.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1014..1304)
                     /locus_tag="ACHUT9_004168"
     CDS             complement(1014..1304)
                     /locus_tag="ACHUT9_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFR1350499.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1738..2448
                     /locus_tag="ACHUT9_004169"
     CDS             1738..2448
                     /locus_tag="ACHUT9_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFR1350500.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2498..3472)
                     /gene="ghrB"
                     /locus_tag="ACHUT9_004170"
     CDS             complement(2498..3472)
                     /gene="ghrB"
                     /locus_tag="ACHUT9_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFR1350501.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3576..4235)
                     /locus_tag="ACHUT9_004171"
     CDS             complement(3576..4235)
                     /locus_tag="ACHUT9_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFR1350502.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
BASE COUNT         1071 a         1036 c         1050 g         1150 t
ORIGIN      
        1 agaaacaaac ttattaagct agaatagcca cgggtgcttg agactgtttg tctcaggtat
       61 tcaccgaaag gcagacagag aaaagcccca cctgactata aatcaaagtg aggctaccct
      121 atgcctgaac accataaggt tagcctctta ctcgttggaa atcaacacag gaggctggga
      181 atgccgcaga aatatagatt actttcttta atagtgattt gtttcacgct tttatttttc
      241 acctggatga taagagattc actgtgtgaa ttgcatatta aacaggagag ttatgagctg
      301 gcggcatttt tagcctgcaa tttgaaagag taagagtctt tggcgggaag ttattcccgc
      361 ctttcttacg gcgttgcgca ttctcattgc acccaaattt attcttcaca aaaataatac
      421 taaattttat tacgcgatcg attatttatt tcctgaaaaa caaataaaaa aatccccgcc
      481 aaatggcagg gatcttagat tctgtgcttt taagcagaga ttacaggctg gttacgttac
      541 cagctgccgg gcctttagcg ccgctttcga tggtgaagga cactttctga ccttcgtcca
      601 gagatttgta accatcgttc tggatagcag agaagtgtac gaacacatct ttagagccat
      661 cgtcaggagt gatgaagccg aagcctttgt cagcgttgaa ccattttacg ataccagtca
      721 ttttaccgga catagtgtat tacctttaat aattaagtgt gcctttcggc gatatggcgt
      781 gctttacaga ttttgaagcg ttaaaggaat gtgcactacg aggggtatca acgataactc
      841 ttgaagggac ttgccttact acactgcttt aatggtctgt acgtcaaacc gttgatgtgc
      901 attaagccac gcattggcgg gtgatgcaac aattattttt catatttatg attaatcggt
      961 tggaaaacgg tcctgtcatc aggaccgtaa acagcaataa agtggataaa agtctattcc
     1021 atcaactgct tacttaatgc cgggttggct tgaatcaaac gcatcaattt tagttcggca
     1081 cttgaaggct tcacgcgtct ggattcccat tcctttacca tggcgactga tacgcccaaa
     1141 acccgggcga aatcatcgat ttttaaccct gtcccttttc gtaactgctc aatttcggta
     1201 caggacgttg ttctgtgcgt cagggtaatt ttctgcgttt catctttaaa aacaatctgt
     1261 tccaggctgc tcaacagctc atgcattgga tctttatatt ccattgaaaa ctcctcaaat
     1321 cacactgcgg gatcgtgaat tgcatcgaag ctcattaaga atagtcggga aaagcagaca
     1381 cagcggggtg aagtcagggc atgctctggc tatgattcga ttcagcctgt catttagcgt
     1441 gacttatagc cacctaacga tctgataaat gccgatatat ctcttaccac tcctgttttg
     1501 taaagatttc gaaaatctaa ccttctgcga aggcgtaaat ctctggctac aaaaaaggca
     1561 acggcactat cattttttaa caatgaaaca acaaattaac cttgtttcat tttctccatg
     1621 attttaccag gaagattacg tagaaagggt atcctgcaag cctgtcctgg actatccttg
     1681 tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg gtgaaaggag taagaaaatg
     1741 gcgacaggaa agtcctgctc tcgctggttt gcgcctcttg cggcgttatt aatggtagtt
     1801 agcctgagtg ggtgttttga taaagaaggc gatcagcgta aagcgtttat cgacttcctg
     1861 cagaatacag tgatgcgtag cggtgaacgt ctaccaaccc tgactgccga tcagaaaaaa
     1921 cagtttggtc cttttgtctc tgattacgcg attctgtatg gttattctca gcaggtgaat
     1981 caggcgatgg attctggtct gcgcccggtc gtagacagcg ttaacgcaat tcgcgtaccg
     2041 caggattacg ttacgcaaag cggtccgctg cgtgaaatga atggttcgct gggtgtgctg
     2101 gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg cgcactctgc gctaaaacag
     2161 agcgatgacc tgaaaccggt atttgatcag gcattcacca aagtggtaac gacgccagct
     2221 gatgcattgc aaccgttgat tccggcggca caaactttca cgcaacaact ggtgatggtt
     2281 ggagactaca ttgctcagca aggtactcag gtgagctttg tcgcaaatgg catccagttc
     2341 ccgacttcac agcaggcaag tgaatataac aaactgattg cgccattacc ggcacaacat
     2401 caggcgttta atcaggcctg gactacagca gttactgcaa cccaataaag agtaaaagcc
     2461 cgagcgatta tcgggcttta cttcacgcag tcgcggctta gtccgcgacg tgaggattca
     2521 cacagttctt ctcaaccttt ccttgtaacg catcaattaa attatccacg gcacaggcgg
     2581 ccatgccata acgcgtctca tgggtggcag atccaatatg cggtactgcg acgacgttgg
     2641 ccattgagag caacggcgaa tctacggaca gtggctcttg ttcgaagaca tcaagcccgg
     2701 cggcgtgaat ttcccctttc tgtaatgctg cgatcagtgc attttcgtca accaccgggc
     2761 cacgtccggc attaatgaaa atggcggagg atttcatttt ggcgaattgt tctgcgccaa
     2821 acagatgatg cgtctcatca gttaacggca ggatcaggca aacgaaatct gactcttgta
     2881 acagtgtatc caaatcgcag tagcgggcgt tgaagcgttc ttctgcttct ttatggtggc
     2941 ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg cgcacgttgt gccagcgcca
     3001 taccgatccg tcccatcccg acaatgccca gtgttttatg gtgaacgtca gtgccgtacc
     3061 agtccgggcc tatgctcgcg gtccattcgc ctgcttttac ccgttctgct acctccacaa
     3121 cccgacgagc ggtagacaac accagcgcca tcagcgtatc ggcgacggtt tctgttaata
     3181 cggttggcgt gtgcatcagc agaatttttc gggcggtaag cgcatcgaca tcaaaattgt
     3241 catagccgac ggagatcgtt gatgtggcat gcagtttcgg cattttttcc agcaatgcgg
     3301 catcaacatt ctcgtttgaa cccagtaaac cttcagcttc ggcaaaaatt gctgcatttt
     3361 gttcgacggt ttgtgggctg aggtttgcca cctggtgaac ggtgaaatgc tcttgcaggc
     3421 gttgcagtaa atcatcaggt aaggctttgt agaggataac ggacggcttc atgcttctct
     3481 ccattcactg ataaggcgat caccttagcc tgaaatatga tgaaggaaaa ctgaagaatg
     3541 ccaggtgatt tctgcatcac ctggcatgaa agggattaca gcgggcttaa ggtaatttct
     3601 acacggcggt tttgcgcctt accttctgcg gtgctgttgc tggcgattgg gttagccggg
     3661 ccaaggccct gagtacggat gcggctggcg tccacgccct gggtgatcaa cgcgctggca
     3721 acggaatccg cacgctgctg ggagagacgc atgttcaggt cgtgaccccc cgtgctgtcg
     3781 gtataaccaa tcacgttaac cgccgttttc ggatactctg tcagcaccat tgccacgccg
     3841 gtcagggtgt tagcgcctgc cggtttcagg gtcgcgctgc tgctgtcgaa ggtcacattg
     3901 ttcggcatat tgaggataat gttatccccg ctgcgggtta cgctaacacc agtgccgcgc
     3961 attttgtcac gcagcttcgc ttcctgcaca tccatgtaat aacccacgcc gccgcccaga
     4021 gctgcgcctg ctgctgcgcc aatcagcgcg cctttaccgc gatctttctt cgaagaagag
     4081 agcgcaccaa tacccgcgcc cacgagagag cccagacctg cgccgatagc agatttgcct
     4141 gcttcgcgtt cgccggtgta agggttagtt gtgcagccag ataccgccag agcgccactc
     4201 actacggcgg caataagata aacacgtttc ttcattgtta atccttaata acctttttat
     4261 tctttgccac gggttccgtg gcgggagatt atgccgcgtg aacatga
//
LOCUS       DAZPEP010000248         4209 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZPEP010000248 DAZPEP010000000
VERSION     DAZPEP010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.250"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(167..577)
                     /locus_tag="ACHUT9_004172"
     CDS             complement(167..577)
                     /locus_tag="ACHUT9_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFR1350503.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSGELSFYEYRKNLATAGVFR
                     WITNIHENKRYYYTFDNSLLFTENIQNTTQIFPH"
     gene            complement(635..868)
                     /locus_tag="ACHUT9_004173"
     CDS             complement(635..868)
                     /locus_tag="ACHUT9_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFR1350504.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            1257..1802
                     /locus_tag="ACHUT9_004174"
     CDS             1257..1802
                     /locus_tag="ACHUT9_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFR1350505.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1777..3702
                     /locus_tag="ACHUT9_004175"
     CDS             1777..3702
                     /locus_tag="ACHUT9_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1350506.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3699..3905
                     /locus_tag="ACHUT9_004176"
     CDS             3699..3905
                     /locus_tag="ACHUT9_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFR1350507.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3902..>4209
                     /locus_tag="ACHUT9_004177"
     CDS             3902..>4209
                     /locus_tag="ACHUT9_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1350508.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     "
BASE COUNT         1029 a          972 c         1160 g         1048 t
ORIGIN      
        1 cgtcgccgta ttcccgtatt aacagagacc gtagcccgac ggggaactcc ttctgcgcga
       61 gtgtgcggga ataatcaaaa acgatgcaca ccggggttac cgggtacaca tatttcatca
      121 tgccagcgag tccggttctg gcacggaaga aaccggacgt tatgatttag tgcggaaata
      181 tttgtgtagt gttctgtatg ttctcagtaa agagtaatga attatcaaag gtatagtaat
      241 accttttgtt ttcgtggata tttgtaatcc atctgaaaac ccctgctgta gcaagatttt
      301 tcctgtattc gtaaaatgat aactctcctg atttgaatcc ttttaaggtg gcttctataa
      361 ggcatttatt ttttgaaaat cttacattta caaccttacc ctgtcctttt attaaaaccg
      421 tattatcgtt ttcaagaaca agatgaatat tctctgtggc taaatagtaa atgtaatgtg
      481 agacattgtg acgttttagt tcagaataaa accagtgata gtttaaatta tttcgcactt
      541 tatcgaatat ttgtttaaaa atggcaacct gagccattgt agtaccttcc atgtgatatg
      601 agggggcgta gtctgcacga ttatctaaat tgcttcaatc tggtctaacc tgttttctga
      661 gcaattcagt aatgtcactc ttttctttgt ttgcttcagg cgaaactctt ttttctgagc
      721 acagtctccg gcggcaggct tcaatgaccc aggctgagaa attcccggac cctttttgaa
      781 caagagcgat gttaatttgt tcaatcattt ggttaggaaa gcggatgttg cgggttgttg
      841 ttctgcgggt tctgttcttc gttgacatga ggttgccctg tattcagtgt cgctgatttg
      901 tattgtctga agttgttttt acgttaagtt gatgcagatc aattaatacg atacctgcgt
      961 cataattgat tatttgacgt ggtttgatgg cgtagatgca cgttgtgaca tgtagatgat
     1021 aattattatc attttgcggg tcctttccgg cgatccgaca ggttacgggg cggaaggcgc
     1081 gcgggttttc gctatttatg acaattttcc ggtttaaggc gtttccgttc ttcttcgccg
     1141 taacctaatg tttttattta aaacaccccc tgaaaagaaa ggaaacgaca ggtgctgaaa
     1201 acgggctttt tggcctctgt cgtttccttt ctctgttttt gtccgtggaa tgaacaatgg
     1261 aagtcaacaa aaagcagctg gctgacattt tcggtgcgag tatccgtacc attcagaact
     1321 ggcaggaaca gggaatgccc gttctgcgag gcggtggcaa gggtaatgag gtgctttatg
     1381 actctgccgc cgttataaga tggtatgccg aaagggatgc tgaaattgag aacgaaaagc
     1441 tgcgccggga agttgaagaa ctgcggcagg ccagcgagac agatctccag ccagggacta
     1501 ttgagtacga acgccatcga cttacgcgtg cgcaggccga cgcacaggag ctgaaaaatg
     1561 ccagagactc cgctgaagtg gtggaaaccg cattctgtac tttcgtgctg tcgcggatcg
     1621 caggtgaaat tgccagtatt ctcgacggga tccccctgtc ggtgcagcgg cgttttccgg
     1681 aactggaaaa ccgacatgtt gatttcctga aacgggatat catcaaagcc atgaacaaag
     1741 cagccgcgct ggatgaactg ataccggggt tgctgagtga atatatcgaa cagtcaggtt
     1801 aacaggctgc ggcattttgt ccgcgccggg cttcgctcac tgttcaggcc ggagccacag
     1861 accgccgttg aatgggcgga tgctaattac tatctcccga aagaatccgc ataccaggaa
     1921 gggcgctggg aaacactgcc ctttcagcgg gccatcatga atgcgatggg cagcgactac
     1981 atccgtgagg tgaatgtggt gaagtctgcc cgtgtcggtt attccaaaat gctgctgggt
     2041 gtttatgcct actttataga gcataagcag cgcaacacac ttatctggtt gccgacggat
     2101 ggtgatgccg agaactttat gaaaacccac gttgagccga ccatccgcga tattccgtcg
     2161 ctgctggcgc tggctccgtg gtatggcaaa aagcaccggg ataacacgct cactatgaag
     2221 cgtttttcca atggtcgtgg cttctggtgc ctgggcggta aagcggcaaa aaactaccgt
     2281 gaaaagtcgg tggatgtggc gggttatgat gaacttgctg cctttgatga ggatattgaa
     2341 caggaaggct ctccgacgtt ccttggcgac aaacgtattg aaggctcggt ctggccaaag
     2401 tccatccgtg gctccacgcc caaagtgaga ggcacctgcc agattgagcg tgcagccagt
     2461 gaatccccgc attttatgcg ttttcatgtt gcctgcccgc actgcgggga ggagcagtat
     2521 cttaaatttg gcgacaaaga gacgccgttt ggcctcaaat ggacgccgga tgacccctcc
     2581 agcgtgtttt atctctgcga gcataatgcc tgcgtcatcc gccagcagga gctggacttt
     2641 actgatgccc gttatatctg cgaaaagacc gggatctgga cccgtgatgg cattctctgg
     2701 ttttcgtcat ccggtgaaga gattgagcca cctgacagtg tgacctttca catctggaca
     2761 gcgtacagcc cgttcaccac ctgggtgcag attgtcaaag actggatgaa aacgaaaggg
     2821 gatacgggaa aacgtaaaac cttcgtaaac accacgctcg gtgagacgtg ggaggcgaaa
     2881 attggcgaac gtccggatgc tgaagtgatg gcagagcgga aagagcatta ttcagcgccc
     2941 gttcctgacc gtgtggctta cctgaccgcc ggtatcgact cccagctgga tcgctacgaa
     3001 atgcgcgtat ggggatgggg gccgggtgag gaaagctggc tgattgatcg gcagattatt
     3061 atgggccgcc acgacgatga acagacgctg ctgcgtgtgg atgaggccat caataaaacc
     3121 tatacccgcc ggaatggtgc agaaatgtcg atatcccgta tctgctggga tattggcggg
     3181 attgacccga ccattgtgta tgaacgctcg aaaaagcatg ggctgttccg ggtgatcccc
     3241 attaaagggg catccgtcta cggtaagccg gtggccagca tgcctcgtaa gcgaaacaaa
     3301 aacggggttt accttaccga aatcggtacg gataccgcga aagagcagat ttataaccgc
     3361 ttcacactga cgccggaagg ggatgaaccg cttcccggtg ccgttcactt cccgaataac
     3421 ccggatattt ttgatctgac cgaagcgcag cagctgactg ctgaagagca ggtcgaaaaa
     3481 tgggtggatg gcaggaaaaa aatactgtgg gacagcaaaa agcgacgcaa tgaggcgctc
     3541 gactgcttcg tttatgcgct ggcggcgctg cgcatcagta tttcccgctg gcagctggat
     3601 ctcagtgcac tgctggcgag cctgcaggaa gaggatggtg cagcaaccaa caagaaaaca
     3661 ctggcagatt acgcccgtgc cttatccgga gaggatgaat gacgcgacag gaagaacttg
     3721 ccgctgcccg tgcggcactg catgacctga tgacaggtaa acgggtggca acggtacaga
     3781 aagacggacg gcgagtggag tttacggcca cttccgtgtc tgacctgaaa aaatacattg
     3841 cggagctgga ggtgcagacc ggcatgacac agcgacgcag gggacctgca ggattttatg
     3901 tatgaaaacg cccaccattc ccacccttct gggaccggac ggcatgacat cgctgcgtga
     3961 atatgccggt tatcacggcg gtggcagcgg atttggtggg cagttgcggg cgtggaaccc
     4021 accgggtgaa agtgtggatg cagccctgct gcccaacttt acccgtggca atgcccgcgc
     4081 agacgatctg gtacgcaata acggctatgc tgccaacgcc atccagctgc atcaggatca
     4141 tatcgtcggg tcttttttcc ggctcagtca tcgcccaagc tggcgctatc tgggcatcgg
     4201 ggaggaaga
//
LOCUS       DAZPEP010000249         4179 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZPEP010000249 DAZPEP010000000
VERSION     DAZPEP010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.251"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1358
                     /gene="dcuC"
                     /locus_tag="ACHUT9_004178"
     CDS             <1..1358
                     /gene="dcuC"
                     /locus_tag="ACHUT9_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFR1350509.1"
                     /translation="VVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSSQ
                     ASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQY
                     INSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASPA
                     AIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEHI
                     SHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILEF
                     LRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSFG
                     SASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQASN
                     LGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVTG
                     K"
     gene            complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="ACHUT9_004179"
                     /gene_synonym="citB"
     CDS             complement(1399..2079)
                     /gene="dpiA"
                     /locus_tag="ACHUT9_004179"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFR1350510.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="ACHUT9_004180"
     CDS             complement(2048..3706)
                     /gene="dpiB"
                     /locus_tag="ACHUT9_004180"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFR1350511.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLNVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            4086..>4179
                     /locus_tag="ACHUT9_004181"
     CDS             4086..>4179
                     /locus_tag="ACHUT9_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350512.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLS"
BASE COUNT         1030 a         1062 c          964 g         1123 t
ORIGIN      
        1 gggttgtggt tattgtgggt gtagctcgct acatcattaa agggtattcc gccactggtg
       61 tgttatttgt cggtggcctg ttattgctga ttatcagtgc cattatgggg cacaaagtgt
      121 taccgtccag ccaggcttca acaggctaca gcgccacgga tatcgttgaa tacgttaaaa
      181 tattactaat gagccgcggc ggcgacctcg gcatgatgat tatgatgctg tgtggatttg
      241 ccgcttacat gacccatatc ggcgcgaatg atatggtggt caagctggcg tcaaaaccat
      301 tgcagtatat taactcccct tacctgctga tgattgccgc ctattttgtc gcctgtctga
      361 tgtctctggc cgtctcttcc gcaaccggtc tgggtgtttt gctgatggca accctatttc
      421 cggtgatggt aaacgttggt atcagtcgtg gcgcagctgc tgccatttgt gcctccccgg
      481 cggcgattat tctcgcaccg acttcagggg atgtggtgct ggcggcgcaa gcttccgaag
      541 tgtcgctgat tgacttcgcc ttcaaaacga cgctgcctat ctcaattgct gcaattatcg
      601 gcatggcgat cgcccacttc ttctggcaac gttatctgga taaaaaagag cacatctctc
      661 atgaaatgtt agatgtcagt gaaatcacca ccactgctcc tgcgttttat gccattttgc
      721 cgttcacgcc gatcatcggt gtactgattt ttgacggtaa atggggtccg caattacaca
      781 tcatcactat tctggtgatt tgtatgctga ttgcctccat tctggagttc ctccgcagct
      841 ttaataccca gaaagttttc tctggtctgg aagtggctta tcgcgggatg gcagatgcgt
      901 ttgctaacgt ggtgatgctg ctggttgccg ctggggtatt cgctcagggg cttagcacca
      961 tcggctttat tcaaagtctg atttctatcg ctacctcgtt tggttcggcg agtatcatcc
     1021 tgatgctggt attggtgatt ctgacaatgc tggcggcagt cacgaccggt tcaggcaatg
     1081 cgccgtttta tgcgtttgtt gagatgatcc cgaaactggc gcactcttcc ggcattaacc
     1141 cggcgtattt gactatcccg atgctgcagg cgtcaaacct gggtcgtacc ctatcacccg
     1201 tttctggcgt agtcgttgcg gttgccggga tggcgaaaat ctcaccattt gaagtcgtaa
     1261 aacgcacctc ggtgccggtg cttgttggtt tggtgattgt tatcgttgct acagagctga
     1321 tggtgccagg aacggcagca gcggtcacag gcaagtaaat agtaatgccg gaggcaagtt
     1381 tcctccggct ttatcatgtc acccactgtg gtatatgcgt tgtggtctgc caactttgcc
     1441 gtgaacaatt tcagcaataa tcagatggcg gctggcgcaa tattcaagat aacgcctggc
     1501 agtggtgcag ctgatggtca gtgcctgcgc caccgtttct gccgtatgtt gcacaccagg
     1561 ctctttaaac agttttcgca ccgcgtttag cgtcaagggg tcaatgccgg tcggtagctc
     1621 gtccttaggt tcaccgcgag cataagcatt aaacatctca tcaatttgct tctggctggc
     1681 gctatcaata ctttccagca tatgtttacg ctggcggaaa cgggttagcg tttgccccag
     1741 ccgttcatag gcaatgggct taatgagata atcaaataca ccacaacgta cggcttcaga
     1801 caccgtttcc atatcgctgg ctgcagtggt aaacaccacg tcgccgggat aatgcgcctg
     1861 caccagttca tgcagtaaat taatccctct accgtcagga agatagttat cgagcaagat
     1921 tagccccggc ttaaaacgct cgatcatcat tcgggcctgc gccagatttc ccgccagtaa
     1981 tatctgactg aatccgggaa tgtgacgaat atattccgca tgcatctctg ccagcggcgt
     2041 ttcgtcctca acgatcaata gggttaatgg agctgtcatt aggtttcact ttcggaatat
     2101 agattgaaaa taaggtaccg cagggatcat tatcttcgag agtgataaca ccaccgcagc
     2161 gcgttacgta gctggcaatc aagtacaacc caatgccatg ttcaccgggc tcgtcagcac
     2221 gcgtactgac cccctgctca aatattttgt ctcttagaga ctctggaacg ccgcagccct
     2281 gatcggcgac ttcaatcacc acatcatcgc cttcatcgct gaggaataat tcaacgatct
     2341 tgtttccttc atcgctacgc aggctggctt cgaaggcgtt atcaagtaaa ttgccaacaa
     2401 tggcggcaaa ctcggtgcta tccagtcctg gcggcagttg cgaaagctgg ctaccgggga
     2461 cgatgatcat ttttagcccc agttcgcggg cgcgctgcac tttaccaaaa agcagccccg
     2521 ccacctggcg atcggcaaac gcctcacgca ggctgtcaat aagctgttgc tgggcctgag
     2581 actccccctg caccatggcc agcacgcgat catactcttt catctgcaac agaccattga
     2641 gcgtcgacat ccaattgaga tgctcgtgtc gcaatgtacg aaggctctcg acgtattgtt
     2701 ttatttgcgt cagttgcgca ttgagggtgg aaatttcgtc tttactacga aagctgataa
     2761 tggcccccag caaatcatca cctgaacgaa tagcttcccg gttggcaata acgttcagac
     2821 cattaaagtt cgccaccaca tcctgacgtt tttcatcaat ctgttcggta aagaaatcgg
     2881 cgggcctgac cacttcagca atgggtttac ccaaccattg ccgtccggga gagctcagcc
     2941 ccagcatctt tcttgcgtta cgattgatgg ctgtaatgta accatgcgga tccaccgcaa
     3001 tcagcccttc ataaaccgaa ctaaacagcg cctcttgctg acggaccacg cgtgcgattt
     3061 gctttggctc catgcccatc atttgtcgac ggatatgcgc ggccaggaac cacgacagca
     3121 acatcagaat ccctaacagc acgacaaaca cgcctgccat cggtaataaa aactcagccc
     3181 gccagctatc gattttactc accaggtagc caatcgacac cacgccgatg acttttccat
     3241 cgtcatcaaa gattggcgtt ttggcgcgca tcgccatccc cattgcccct ttcccggtaa
     3301 tgaagtagct ctcccctttc tccagcgcgc cctgtttggt gaactgcata ggataaccaa
     3361 ttttctccgg attaagatgg taaaggcgga tcgagtgccg gtcgccaatc accacataat
     3421 caaaatcggt atctctttgt aatttgttag cgatggtcgc cagccgtttg tagtcacgcg
     3481 ttttcaccgc agagatgaca ctgtcattgg aggcaataat tttcgcctga ttcattgcca
     3541 tgtcgcgtac atgaagcgtt aaatagtcct caaaactggc cgtaaaatat tgcgccaggg
     3601 ctgcaatgac aaatattgag aacaccagaa tcagcaaaaa gatacgcagc ggaaatgcca
     3661 gtctttggaa aaatgcaaac tgtttattct cgttaagctg caacattgtt ttccttgcgg
     3721 gttgtcccga gcctctttga cggatgtaat taaaatgttg aatcaaaata catagatatt
     3781 aattatgatc gtggcaaatt ttattcatta aatcaattaa atcacttaaa cctgcaggtc
     3841 atttcgtaat tacattaatt aaaaaactta aaaccattaa ataaataaaa aaccactaac
     3901 tctatgtgaa ataaatcaaa atttcacgct gcaatactcc ttagaatgta tagcgaaagg
     3961 agaaaaagat atacctcgat cacccccctt tctcccaagt gaaaataaaa ggttatcaac
     4021 ttgcaacatt gaacaacatt cgttgcaaat cgataacaac atgcaccttc aggatactat
     4081 ttattatgtt cggcaatgat attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg
     4141 cggaaatcgc ccaattcctg catgaaaatg atttgagcg
//
LOCUS       DAZPEP010000250         4165 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZPEP010000250 DAZPEP010000000
VERSION     DAZPEP010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.252"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            426..1592
                     /gene="sopA"
                     /locus_tag="ACHUT9_004182"
     CDS             426..1592
                     /gene="sopA"
                     /locus_tag="ACHUT9_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFR1350513.1"
                     /translation="MSLKKLLQDCVTRGQEMTQAIAIAQFGDDSPEARRITRRWGITE
                     VADLIGVTPQAIRDAEKSGRLPAPDFEMRGRIERRAGYTIDQINSMRAVFGNPNQRPQ
                     DKNPVVLAVMSHKGGVYKTSSAVHQAQWLALQGHRVLLVEGNDPQGTASMYHGYVPDL
                     HIHAEDTLLPFYLGERDNAEYAIKPTCWPGLDIIPSCLALHRIETDLMQYHAQGKLPH
                     PPHLMLRAAIESVWDNYDVIVIDSAPNLGTGTINVVCAADVIVVATPAELFDYASVLQ
                     FFTMLLDLLESVDLGGFEPTVRLLLTKYSLTTGNQSRWMEEQIRNTWGSMVLRQVVRV
                     TDEVGKGQIKMRTVFEQAANQRSTLNAWRNAVSIWDPVCREIFDDLIKPRWENE"
     gene            1592..2557
                     /locus_tag="ACHUT9_004183"
     CDS             1592..2557
                     /locus_tag="ACHUT9_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001728175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFR1350514.1"
                     /translation="MKQRSLLKNAPDINRIMSNNHHAPEHQPVSPMVGDLKRQLSSLT
                     GNSITLPVCGRNVNFKLETIPADKVEMATMVWLGNERDQDLLNESSLADLIPSFLTSG
                     QQNPAFARKVSGIVEVADGSRRRKAAILTGCDYRVLIGDLDDEQMHWLSQIGNDYRPI
                     SAYERGKRYLRKLNDFDGNVKALAEAENIDRNIITRCINTAGLPKDILAIFNHPGELS
                     ARAGDSLFKIYKKNMAAMSNAAHHLLTIKKNGEDLEASRIIQILSDSVLVGDEEKTKD
                     EKKYGEGITARYKGNFVTIKIDSRKISKEILSKIELLLEEQSTDN"
     gene            complement(2590..3165)
                     /locus_tag="ACHUT9_004184"
     CDS             complement(2590..3165)
                     /locus_tag="ACHUT9_004184"
                     /inference="COORDINATES: protein motif:HMM:NF014467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1350515.1"
                     /translation="MDFRMSEQPRTITIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDDASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEYQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     KSLLAAWKTYRVLLNRVDTSLSPDIEWPSQP"
     gene            complement(3174..4076)
                     /locus_tag="ACHUT9_004185"
     CDS             complement(3174..4076)
                     /locus_tag="ACHUT9_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HFR1350516.1"
                     /translation="MITLILSAPVTEMAEAFKRVFANADNVNIVGKPFETIREFDCMV
                     SAANSFGLMDGGVDAAITAFFGTQLQSRVQNHILREYLGEQPVGSAFVIETGHNHHPW
                     LVHAPTMRVPLTIDGTDAVYNATWAALLAIFQHNKHATTDRKIKTVVFPAMGAGCGQV
                     PFESVARQMKQAWDNFNKKTASINWEYAQSRQSAVFGTYAYCPGNSVCRYADTKYIGC
                     GDYRTYCSRSGQVCINPEHQADDVLIQHQANNRFRPGSHIHRINPENPVGNVISGAHS
                     HGSSIVIGAHTHTLNKQYSVSDIK"
     gene            complement(4073..>4165)
                     /locus_tag="ACHUT9_004186"
     CDS             complement(4073..>4165)
                     /locus_tag="ACHUT9_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1350517.1"
                     /translation="SHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT         1198 a          856 c          874 g         1237 t
ORIGIN      
        1 ttgattagtg ttctcattta gtgtaatatt ctcattttgt gtactgcttt atttaacatt
       61 tagctttaat tctttattta tgttctttat tatgtttgca tcattactta gtttttgtgt
      121 tctttatttg tgttcttgta ttggtaaggt tgggagaact aatagagaaa acgtatctca
      181 ttgatgattc ttatcatgtt tccctagttt tacatgattt cctttcttca gaaaatataa
      241 taaatatcaa ttacttatga ttatgttgtt gttcgtttta tgcatttcgg gaggggaaca
      301 aaagatgatt tagcttaaaa attctccttt gcgtttttct ttttgctttg cctttttcat
      361 gcaaatttgc tagaaataag caaagattta attattaagc aaagttacaa gctggagatg
      421 acagtatgtc tttgaaaaag ttattgcaag attgtgttac gagaggtcaa gaaatgaccc
      481 aagcaattgc tatcgctcaa ttcggtgatg atagtcctga agctcggcgt atcacccgcc
      541 gatggggtat tactgaagta gcagatttaa ttggtgtaac tcctcaagca attagagatg
      601 ctgagaagag cgggcgttta cctgcacctg actttgaaat gagaggaaga attgaacgtc
      661 gtgctggtta taccatagat caaatcaaca gtatgcgagc agtttttggc aacccaaacc
      721 aacgaccaca agataaaaac cctgttgttc ttgccgttat gtcacacaaa ggcggcgttt
      781 ataaaacatc gtctgcggta catcaagctc aatggttagc attacaaggt catcgtgttc
      841 ttttggttga aggtaacgat cctcaaggta cagcctctat gtatcacggt tatgttcctg
      901 atttacatat ccatgccgag gatactttac tgccatttta cttaggagaa cgcgataacg
      961 ctgaatatgc tataaagcca acatgttggc ctgggctaga cattatccct agctgcctag
     1021 ctttacatcg tatagaaaca gatctcatgc aatatcatgc tcagggtaaa ttgcctcatc
     1081 ctccacacct tatgctgcgc gcagctattg aatcagtttg ggataactac gatgttatcg
     1141 ttatagatag tgctccgaac ctcggtacag gaaccattaa cgttgtttgt gccgctgacg
     1201 ttattgttgt tgctacacct gcagaattat ttgattacgc ttctgttcta cagtttttca
     1261 caatgcttct cgatctattg gaaagcgttg acctaggtgg atttgagcca accgttcgct
     1321 tacttctgac taaatatagt ctcactacag ggaatcaatc acgatggatg gaagaacaaa
     1381 taagaaatac atggggatca atggtattac gtcaggttgt acgcgttacc gatgaagtag
     1441 gtaaaggcca gataaaaatg agaacagtat ttgaacaagc agcaaaccaa cgttcaacat
     1501 tgaatgcatg gagaaatgct gtttctatat gggaccctgt ttgcagagaa atttttgatg
     1561 atctaataaa acctcgctgg gagaacgaat aatgaaacag cgctctcttc taaaaaatgc
     1621 tccagatatt aatcgcatta tgagtaataa tcatcatgcc cctgaacatc agcccgtatc
     1681 acctatggtt ggtgatttaa aacgtcaact gagttcatta accggtaaca gcataacttt
     1741 gccagtatgc ggacgtaatg ttaactttaa gttagagaca attcccgcag ataaagttga
     1801 aatggcaaca atggtatggc taggtaatga acgcgatcaa gatttattaa atgaatcctc
     1861 tctagccgat ttaatcccat cttttctaac gtctggacaa caaaatccag ctttcgccag
     1921 aaaagtatct ggaatcgttg aagtcgctga cggctcacgt agaagaaaag cagcgatatt
     1981 aacaggatgt gactatcgcg tcttaattgg tgatctcgac gatgaacaga tgcattggtt
     2041 atcccaaatt ggtaacgatt ataggcctat tagtgcatac gaaagaggaa agagatactt
     2101 acgcaagcta aatgactttg atggtaacgt taaagcattg gccgaggctg aaaatataga
     2161 tagaaatatt atcactcgct gtattaatac agctggtcta ccaaaagata tattagctat
     2221 cttcaatcat ccaggagaac tgtcagctcg tgctggagat tctttgttta agatttacaa
     2281 aaaaaacatg gctgccatga gcaatgctgc tcatcatctt ctgacaataa aaaaaaacgg
     2341 cgaggattta gaagcttcac ggattataca aatattatct gactctgttc tggttggcga
     2401 cgaggaaaaa acaaaagacg agaaaaaata tggggaaggg atcacggcta gatataaagg
     2461 taattttgta actataaaaa ttgatagccg caaaatatct aaagaaatac taagcaaaat
     2521 tgagttgtta ctagaggaac aatctactga taattaagaa ttatcccctg cattgcaggg
     2581 gatattgatt tatggttgac ttggccactc gatatctgga gatagtgatg tatcaacacg
     2641 attcaacaac acccgatatg tcttccatgc ggccagtaac gatttctctt cctccgttgc
     2701 gatctcaaga tctaccgcat cctgaagcgg cgcaatatgc tcactggcta cctgcatcag
     2761 gctgtttttt gtttcttccg cctcccggat ccggaacagt ttttctgctt ctgcatcttt
     2821 cacccaggct gtgccgttcc acttctgata ttccccttcc ggcgataacc aggtaacatt
     2881 ttccggtaac ggaccgagtt cagaaataaa taacgcgtcc cctgatgcca cgtcataaac
     2941 tgttttaccc cgatggtctt caacaagatg ccacgatgca tcatcactgt tgaaaacagc
     3001 cacgaagcca gccggaatat ctggcggtgc aatatcggta ctgtttgctg gcagacctgt
     3061 atgaggcgga atgtatgcgt caccttcacc aataaattca ttagttccgg ccagcagatt
     3121 ataaattgtt atggtccgtg gttgttcact cattctgaaa tccatgttca cctctactta
     3181 atatcagaga cagaatattg tttattgagc gtatgggtgt gagcaccaat aacgatgcta
     3241 cttccgtggc tatgtgcgcc agagatgaca ttacctacgg gattttctgg attaatccgg
     3301 tgtatatgcg aaccagggcg aaaccggtta ttagcctgat gttgtatcag tacatcatca
     3361 gcctgatgtt cagggttaat acagacctgc ccggaacgtg agcaatacgt ccgataatcg
     3421 ccgcatccaa tatatttagt atccgcataa cggcaaacag aattacccgg acagtatgca
     3481 tatgtgccaa atactgccga ctggcgggat tgtgcgtatt cccagttaat tgatgctgtt
     3541 tttttattaa agttatccca tgcctgcttc atctgccggg caacactttc aaacggcacc
     3601 tgaccacatc cggcccccat tgcagggaat accaccgttt ttattttcct gtctgtcgtt
     3661 gcgtgtttat tgtgctgaaa gatggcaagc agagcggccc aggttgcgtt atatacagcg
     3721 tctgttccgt caattgtcag cggaacacgc attgttggcg catgtaccag ccaggggtga
     3781 tgattatgcc ccgtttcaat aacaaatgca gaacctacag gctgctcgcc tagatattca
     3841 cgaagaatat gattctgaac gcgggactgt aactgagtac cgaagaatgc ggtaatggcg
     3901 gcatcaacac cgccatccat caggccgaaa ctgtttgccg cacttaccat gcagtcaaat
     3961 tccctgattg tttcaaatgg ctttccgaca atattcacat tatctgcgtt tgcaaatacc
     4021 cgcttaaatg cttcagccat ttctgttact ggcgcagaaa gaatgagcgt aatcatgcaa
     4081 gcctcacaat atagttaaat gcgatgtttt tgacggtgtt ttccgcgtta ccagcagcgt
     4141 taacggtgat ggtgtgtcca tgtga
//
LOCUS       DAZPEP010000251         4087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZPEP010000251 DAZPEP010000000
VERSION     DAZPEP010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.253"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..877
                     /gene="adeQ"
                     /locus_tag="ACHUT9_004187"
                     /gene_synonym="yicO"
     CDS             <1..877
                     /gene="adeQ"
                     /locus_tag="ACHUT9_004187"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFR1350518.1"
                     /translation="GLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIIT
                     VLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELA
                     GIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSS
                     VTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMT
                     SSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCV
                     VVVASLFALKIILVD"
     gene            930..1382
                     /locus_tag="ACHUT9_004188"
     CDS             930..1382
                     /locus_tag="ACHUT9_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFR1350519.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1593..2773
                     /gene="nepI"
                     /locus_tag="ACHUT9_004189"
                     /pseudo
     CDS             1593..2773
                     /gene="nepI"
                     /locus_tag="ACHUT9_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHUT9_004190"
     CDS             complement(2814..3107)
                     /gene="yicS"
                     /locus_tag="ACHUT9_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFR1350520.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHUT9_004191"
     CDS             3329..>4087
                     /gene="nlpA"
                     /locus_tag="ACHUT9_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFR1350521.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFLHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          900 a          906 c         1076 g         1205 t
ORIGIN      
        1 tggattattt atcgccttaa tgggattaaa aaatactggc gttattgtcg ccaataaaga
       61 cacgctggtg atgattggcg atttaagttc tcacggcgtg ttgttaggta ttttagggtt
      121 ttttattata accgtgttgt catcacgtca ttttcatgcc gcggtgctgg tttctattgt
      181 ggtgacgtct tgctgtggat tatttttcgg tgatgttcat tttagcggcg tctattccat
      241 tccgcctgat attagcggcg tcattggtga agtagatttg agcggcgcgt taacacttga
      301 actcgccggt atcattttct cctttatgct gatcaaccta tttgattcat caggaacatt
      361 aattggtgta actgataaag cgggcttaat agatggtaac ggtaaattcc ccaatatgaa
      421 taaggcgctg tatgttgata gcgtcagttc ggtggcgggt gcgtttatcg gcacctcgtc
      481 tgttactgcc tatattgaaa gtacttctgg tgtggcagtc ggtggccgca cggggctgac
      541 tgcggttgtg gttggcgtta tgttcctgtt ggttatgttc ttctccccgc tggtggcgat
      601 ggttcctcct tacgcaaccg ccggagcgtt aatctttgtt ggcgtgctga tgacttcgag
      661 cctggcgcgc gttaactggg atgattttac cgaatcggtg cctgcgttta ttaccacggt
      721 gatgatgccc tttactttct cgatcaccga agggattgca ctcggcttta tgtcgtactg
      781 catcatgaaa gtgtgcaccg ggcgctggcg cgatctgaac ctgtgtgtgg tggtggtcgc
      841 atctctgttt gcactgaaga ttattctggt ggattagtaa ctggcgaggg tttccagata
      901 tcatgagttc tgataacgca ggagaactca tgatctggat aatgctcgcc acgctggcgg
      961 tagtgtttgt ggttggtttt cgggtgctga catccggggc cagaaaagcg attcgccgtc
     1021 tcagtgatcg gctgaatatc gatgtcgtac ccgtggagtc gatggtcgat caaatgggaa
     1081 agtcagccgg tgacgaattt ttacgttatt tgcatcgccc ggatgagtcg cacctgcaaa
     1141 acgccgcgca ggtgttgctc atctggcaaa ttgtcattgt cgatggtagc gaacagaacc
     1201 tgctgcaatg gcatcggatt ttacaaaaag cccgcctttc cgcgccgatt accgatgctc
     1261 aggtcaggct ggcgctgggt tttctgcgcg aaaccgaacc tgaaatgcag gatattaatg
     1321 cttttcagat gcgctataac gcgttctttc agcctgccga gggcgttcac tggctgcatt
     1381 aaaaccgctt cgtcttatca gcctacagac cgcgcctgac ctgtcgggtg gataagacgt
     1441 tcacgccgca tccggtaatc tttgccctgt tccgtttcga tcttaactcc agctcaagca
     1501 acaccgtaaa agttgcaaaa acgttaaatg cgtcacacat ttcaatgtta aagtggtcgg
     1561 cttttcccct gaaacatgcc acgggtaaca ccatgagtga atttattgcc gaaaaccgcg
     1621 gcgcggatgc catcacccga ccgaactagt cagccgtttt ctcggtggcg ttttgtgtcg
     1681 cctgtctgat tatcgttgag tttttgcccg tcagtttgtt gacgccaatg gcccaggatt
     1741 taggcatttc ggaaggggtt gccgggcaat cggtgaccgt gaccgccttt gtggcaatgt
     1801 ttgccagttt gtttattacc cagacaattc aggctactga ccgccgcaac gttgttattt
     1861 tgtttgccgt tttgctgacg ctctcctgct tgctggtttc ctttgctaac tcattcagtt
     1921 tgcttttaat cggtcgtgcc tgtctggggc tggcgctggg cgggttctgg gcgatgtcgg
     1981 cgtcgctgac catgcgtctg gtgccgccgc gtacggtgcc gaaggcgctg tcggtgatct
     2041 tcggcgcggt ttctattgcg ctggtgattg ccgcgccgtt gggcagtttt ttaggcgagc
     2101 ttatcggttg gcgcaatgtc tttaatgcgg cggcggtgat gggcgtgctg tgtattttct
     2161 ggattatcaa atcattgcct tcactgccag gcgaaccctc gcatcagaaa caaaatactt
     2221 tccgcttatt acaacgtccg ggtgtgatgg cagggatgat cgccatcttc atgtctttcg
     2281 ccgggcagtt tgctttcttc acatatattc gcccggtgta tatgaacctg gcgggattca
     2341 gcgtggatgg cttaacgctg gtgctgttga gttttggcat tgccagcttt atcggtacgt
     2401 cgctttcgtc attcattctt aaacgttcgg taaaactggc cttagcaggc gcgccgttaa
     2461 tactggctgt gagtgcgttg gtgctgacgt tgtggggaag cgataaaatc gttgctaccg
     2521 gcgtggcgat tatctggggg ctaacttttg cattggttcc cgtcggctgg tcaacgtgga
     2581 tcacccgctc actggccgat caggcagaaa aagccgggtc tattcaggtg gcggttattc
     2641 agcttgtggc gcggcaatcg gcggttatgc gctggataat attggtctga cttcgccgtt
     2701 gatgttttct ggcacattga tgttgctgac tgcattgttg gttactgcaa aggtgaaaat
     2761 gaagaaatcc tgatatcagg taaagaagcg ttggtcagta gttgttgctg ttcttacata
     2821 tccgggcatt ctaccttacc caacgcttcc caataattgc tctgattatt acgttccatc
     2881 gccacgatgg cctcgcgtat atgttgttta ttgttttcat ctgacaacat taacttttcc
     2941 cacgcttcat ctgataatga cgcctgtggc gtgcaaattt tacgtaaatc ttgtaatacc
     3001 gtggttttct cttttgcgga ctgcagtgag ctaaaaggcg actcggcgta tacgatgcct
     3061 ggcatagcga aaaaagtgaa aataagcagt agtgtcgttg gtttcatgga ttcaaccttt
     3121 tacaaatgtg tatcgaatga agcataagtg tagtttgctt tcatcgctct catcatgctg
     3181 tacgaaacgc gcttgcgtgg tcagtaagaa gtgccagact ttatattcca cttttattcc
     3241 tttttattct tatcgatagc gtttcgtttt ttaaaccgca gcgaccttac cgctatagtc
     3301 aggtaatcat taataaaagg ataaaaaaat gaaactgaca acacatcatc tacggacagg
     3361 ggccgcatta ttgctggccg gaattctgct ggcaggttgc gaccagagta gcagcgatgc
     3421 aaaacacatt aaagttggcg ttataaatgg cgcagaacaa gatgtcgcgg aagtcgctaa
     3481 aaaggtggca aaagagaaat atggcctcga tgttgaactg gtaggattta gcggttcgtt
     3541 gctaccgaac gatgcgacaa atcatggcga acttgatgcg aatgtcttcc tgcatcgacc
     3601 ctttcttgaa caagataatc aggcgcatgg ttataaactg gtagcggtgg gaaatacgtt
     3661 tgtcttccct atggcgggtt attccaaaaa aatcaaaacc gtggcgcaaa taaaagaagg
     3721 cgcgacagtg gcgatcccca acgacccgac caaccttggt cgcgcacttt tactgttgca
     3781 aaaagagaag ctaattactc tgaaagaggg aaaagggtta ttacctaccg cgctggatat
     3841 aaccgataat cctcgtcatt tgcagattat ggaactcgag ggggcgcagc tcccgcgcgt
     3901 actggatgat cccaaagttg atgtagcgat tatcagcacc acttacattc agcagacagg
     3961 gctttctccg gtgcacgaca gcgtatttat tgaagataag aattcgccgt atgtgaatat
     4021 tttggtggca cgggaagata ataagaatgc agaaaacgtg aaggaatttc tgcaatctta
     4081 tcaatca
//
LOCUS       DAZPEP010000252         4075 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZPEP010000252 DAZPEP010000000
VERSION     DAZPEP010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.254"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUT9_004192"
     CDS             <1..784
                     /locus_tag="ACHUT9_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1350522.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1756
                     /locus_tag="ACHUT9_004193"
     CDS             797..1756
                     /locus_tag="ACHUT9_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFR1350523.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECADLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFESDGKKIEL
                     PGRSSYNLEEMLSAINFTIPYSIASVSDSSNTADIFPGTPFPLLFEIEVSSCTGNNTN
                     CNSVRFNYNLNTILQVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQG
                     ELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSN
                     YYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHUT9_004194"
     CDS             complement(1794..2693)
                     /gene="nhaR"
                     /locus_tag="ACHUT9_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFR1350524.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHUT9_004195"
     CDS             complement(2759..3925)
                     /gene="nhaA"
                     /locus_tag="ACHUT9_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFR1350525.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
BASE COUNT         1224 a          952 c          861 g         1038 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaatagagc ttccaggaag gtcatcttat aatttagaag aaatgctatc
     1141 tgcgataaat ttcaccattc catactctat cgcatctgta tcagatagtt ccaacaccgc
     1201 agatattttt ccaggaactc cttttccttt gttgttcgaa attgaagtga gttcatgtac
     1261 agggaataat actaattgta atagcgtccg ctttaattat aatttaaata ctattttaca
     1321 ggtaaattta atgacctgtg gttttgaaga tcaatccatc gacactggac gatttgtact
     1381 gtctcgtatc aataatgaaa attatcagtt tcatcatata agatttgact gcatccaggg
     1441 cgagcaaggt gaattagttt ttgaacccag tgatgttgaa tattactttg aaccagtgac
     1501 gatacaggag tcaggaacca gcatattaaa aaatgagtta gaaaacagtg aggacggtgc
     1561 gggtcaagta ggctttcaac tctccaatga tggtacgaat gaaatccagt atggtaaaag
     1621 caattactac tcttttcagc atccccatga aggaagtaat cagattcctc tatttattcg
     1681 cccgcgtaca tatggaaata atgtctcaag cgggcaaata atgagccgag tcaaaatcgt
     1741 ggttatgtac aactaatcta tctcccccac cttaactttg ggggaggtgc cgattaacgc
     1801 gccgctggac taaaaagcgc agaataatcc gtattgcaga ttcgctgtac cgccgggtgc
     1861 tgaatcatcc gctcagcaaa aatagcatgg tactcttcca tcacattctc gacgcgacca
     1921 atttctacga cagttttatc ggcataaaag tcatatgcat aaagcgttgg ggcaacgaag
     1981 attgcattgt gcatcgcacc aaaagctttc atcaacgcgg catcatcaaa ctcgccgagg
     2041 atttctacgt ttaatccctg ggagttaaac cagttaagca atttgcgccc taacattgaa
     2101 cgtcgcccag gaatcaaaag tcgccgttct tccagacaag ccgggaacga tttttctggt
     2161 ggtggatttg tacaccagaa actcacgcca cattcgccaa ttctcacgga gaacaggcct
     2221 tcctgctgcg tagagtctat cggacagtca gaaatgatca tatccagttt atgctgactt
     2281 aattgctcca gcagcatttc gtgggtggat tcgaagcagc gaagatgaat gggctcgcct
     2341 tctaccactg cggcgttaag tacgctactg accaggcgtt tggaaagtgc atcagccacg
     2401 ccaacgtcaa acaataaatt ggattctttg cgatagttca caatatccag catttcctgg
     2461 cttaaggtga acattttatc ggcatagcga tagaccagtt ctcccagctc gctgggttcg
     2521 agaccacgtc ccttgcgttt aaataatttg ccttgcaggc gctcttccag cgctcgaatc
     2581 tgtccggtaa tggtttgtgg agttaaataa agcgcctccg ctgcgccaac cacggaacct
     2641 tctttataga catgccagaa gtaatacaag tggttgtaat tgatatgaga catgctcatt
     2701 tctctccctg ataacaatga aaagggagcc gtttatggct ccccggtaaa ccgtcctgtc
     2761 aaactgatgg acgcaaacga acgcgtaacc agctgtatcc aattaccgcc gaagagatag
     2821 aaccgaccag gataccgagt ttcgcccagt taatcagttc tggatctacg ctaccaaagg
     2881 ccaggctggc aataaagata gacatagtaa aaccgatacc gcacaggatc cccaccgcca
     2941 taatttgctg ataagtcgtt ccctcaggca gatgcgccag tttcaaacgc agcgccaacc
     3001 agcagaacag actaatcccc agcggtttgc caatcagcaa gccagcgatg atccccaatg
     3061 gcagaatgga ggtcaagcca tccagcgtga cgccttgcag tgaaacgcca gcattagcaa
     3121 atgcaaacag cggtaaaatc agatacgcca cccacgggtg caatacatgc tccagtcgct
     3181 tcgctggaga acgcccatgc ttctctttca aaggaataaa gaagccgaca attacccccg
     3241 ccagggttgc gtgaaccccc gatttcaaca ccgcagtcca caacaccacg ccaacaagaa
     3301 tatagacgcc cgtgcggcgt acaccacaca gattcaatac cgcgagtacc gcaattgcta
     3361 cagccgcgac gccaagagag gccatcgata agtcattagt gtagaacaat gcgatgataa
     3421 tgatggcccc aagatcgtcg ataatagcca gagccatcaa aaagatcttc agtgctaacg
     3481 gaacacgact tcccaacagc gccagcacac caagtgcaaa ggcaatgtca gtagccgccg
     3541 ggatcgccca cccttcgcgg gtaatcggat cggcatagtt aaaggccaga tagagtaatg
     3601 ccggaacaat catcccacca atagcggcga taaccggaaa tgcggcctgg cgtaagctgg
     3661 ctagcgatcc ttgcatcagt tcacgtttaa cttccagacc gactaacagg aaaaataccg
     3721 ccatcagcgc gtcatttatc cataacagca tgtttttgtt gatttcgagt gaaccaaccc
     3781 ggagctgaac cggcgtctcc agaaagtcgt gataccatcc actggttgcg ccgctgttgg
     3841 ccatcatcat cgccaggata gcggcaatga taagaataat gcctcccgag gcatcactgc
     3901 taaagaatcg atgcagatgt ttcacttttt atttctcttt caggtgaata gatcgttttt
     3961 actattttac cccgcacaaa tcatcgttaa aatcagatta tagtgtagga ataactcgtt
     4021 ttttacgagc aattaaagga tttgcgccat cagcggtgag atgagttttt gacgg
//
LOCUS       DAZPEP010000253         4061 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZPEP010000253 DAZPEP010000000
VERSION     DAZPEP010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.255"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUT9_004196"
     CDS             <1..784
                     /locus_tag="ACHUT9_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFR1350526.1"
                     /translation="FSVSAPGHHSWKRDFDPDSPEIKFSDRREALKLMTQIESTVTSL
                     HREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARLEDEGFTVKITFPAVSQ
                     LQTRIAVKTNLSALMEERTETVTRRRRQSGFWGKICGAFGTSDWGWENYKENVSRSVI
                     NINSVRKEVMSLTRAYFGELQASIEQNINQPVRQEIDDFFCTFREKVEQLRNTLIQSS
                     EDHKRDQQAQEQLTERLQALNERVPELITDSKALREELETLL"
     gene            781..1686
                     /locus_tag="ACHUT9_004197"
     CDS             781..1686
                     /locus_tag="ACHUT9_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFR1350527.1"
                     /translation="MTSPFIQQIADNRVCQVLSCLPEKFVVDFANGIDVAQEHNRTAG
                     GRTFFRRLKEGLTGKGAVRQNAINASLAQGVEASLRWLTELTTSLATTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLAEQVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     NGGSATVRLNTLDWLGGQSREQADNEWHEAVNWLGDWCSEERHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGEA"
     gene            1683..2753
                     /locus_tag="ACHUT9_004198"
     CDS             1683..2753
                     /locus_tag="ACHUT9_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFR1350528.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGAQISMVSGASIG
                     ALNGAIITASPDLSEAALRLEALWDHLGNNQVLSVNRSVYFSLLKKLVQAMNLCQIPG
                     RAGALLTTLFRHISTINGFDNPMIQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGAGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMYGDGGMGGWRNRQGNTPVTPLVDAGCNMVIVTHLSDGSLWDRRAYPDTTILEIRPR
                     KRLKQIGDEGKSGGLLSFTSAHTDAWRQQGYEDTMLTMEHIRKPLAARQALTRSETVL
                     QKSLEITEGADSALRNAMARIK"
     gene            2990..3343
                     /locus_tag="ACHUT9_004199"
     CDS             2990..3343
                     /locus_tag="ACHUT9_004199"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350529.1"
                     /translation="MFSIGNVSINCTINPEDNNLLPHYVWIYGHTSTQLNQYDSDIIF
                     IINDKKYPAPSVDGTRMNKNAWVYFIDAISEATQFDVLVNGKKVDSYTANIKNVKKTL
                     GNKFYDSCWNTWFQE"
     gene            3469..4008
                     /locus_tag="ACHUT9_004200"
     CDS             3469..4008
                     /locus_tag="ACHUT9_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HFR1350530.1"
                     /translation="MNWHYEKNGVRYDNVTEEDIIERIRRGELNASTLVWQQGMLEWQ
                     PVSATSLAEILKQSEVPPALPGHRIPGGVVWTLAFAPLIGYILEMWTAGLNGMSFERA
                     YDAVSEGQYWFISLILNIALGYLDERKLRKSGVDTATFGWLAWLIPVYLWRRAKILGQ
                     KPAYFWVWLVMLILVLFTV"
BASE COUNT         1077 a          900 c         1101 g          983 t
ORIGIN      
        1 gtttagtgtc agtgccccgg gccatcattc atggaaacga gactttgatc cggacagccc
       61 cgagataaaa ttcagtgatc gcagggaagc ccttaaactg atgacgcaaa tcgaatcgac
      121 cgtgaccagc ctgcaccgtg aggctgaagc acagttccgg cctgaactgg aaaaaatcgt
      181 caggggtatc gaaacaggtt ttcgtggtac ggccctgtat gccacagaaa atattgccgg
      241 acgtatcaat gcccgcctgg aggacgaggg cttcactgta aaaatcactt tcccggcagt
      301 cagccagtta cagaccagga tcgcggtaaa aacaaatctg agtgcgctta tggaggaaag
      361 aactgagaca gtcacccgtc gccgtcggca gagtggtttc tggggaaaga tttgtggagc
      421 gtttggcacc agtgactggg gctgggaaaa ctataaggag aatgtgagcc gcagtgtgat
      481 caatatcaac tcggtcagga aggaagtgat gtcactgacc cgggcatatt tcggggagtt
      541 gcaggcatcc attgagcaga atattaacca gcccgtccgc caggagatcg atgatttttt
      601 ctgtacattc agggaaaaag tcgaacagct acgtaacaca ctcattcaga gctctgaaga
      661 tcataaacgc gatcagcagg cacaggagca gcttacggag cgccttcagg cattaaatga
      721 aagggttccc gagctgatta ctgacagtaa ggcgttaagg gaagaactgg agacactgct
      781 gtgacctcac catttattca acaaattgct gataaccggg tatgtcaggt actcagttgc
      841 cttcctgaaa aatttgtggt tgattttgca aacggtattg atgttgcaca ggagcataac
      901 cgcacggccg gggggcgcac gtttttccgg cgtttaaaag aaggtctgac tggcaaaggc
      961 gctgtacgcc agaatgccat caatgcttcg cttgcacagg gcgttgaggc gtccctgcgc
     1021 tggctgacgg agctgacaac gtcgcttgcc accacaaatt acgcgattac ccgggtaaac
     1081 gacagggtca gttcactggt cagtgatact gccaggctgg cgcattattc ggcagacacg
     1141 cgggagcagt tactcaccct ggcagaacag gttcatcaga aactgaatca tcttgaagaa
     1201 aaactccacc gtgttgacca ggttcagcgg gcacagttac atcttgagca gatattctca
     1261 tggtggagcg ccgggcgata cgcgtcattt tcccctgccg gacgttgtta tgtggcgctt
     1321 gaagagcttc gctggggcgc gtttggtgat gtgatacgtc agggtgaaac aggccaggtt
     1381 aaccagttac tggatatcct cagacataaa gcattaacgc aaatggcaca ggagaatggc
     1441 ggtagcgcaa cagtacgtct gaacacactg gactggcttg gtggtcagag ccgggaacag
     1501 gctgataacg aatggcatga agcggttaac tggctggggg actggtgcag tgaagagcgg
     1561 catcctgtga tctggtcaac cacacaggct gcagaacact taccggttcg tatgccccgt
     1621 ctctgctctg cagaacgcct ctctgaaagt atggttgatg aaatatttca gaagggggag
     1681 gcatgagtac agaaatgaaa acggggctgg tgctgtccgg cggcggggcg gtgggcgctt
     1741 atcaggcggg agtggttaag gcactggcag agtgcggcgc acagatcagt atggtctcag
     1801 gagccagtat cggggctctc aatggtgcca ttatcacggc ctctcccgat ctgtcagaag
     1861 ccgccttacg cctggaggcg ctctgggatc atctggggaa taatcaggtt ctgtcggtaa
     1921 acagatcggt ttacttttca ttgctgaaaa aactggttca ggccatgaac ctctgccaga
     1981 tccccggacg tgcgggcgca ctgcttacga cgctttttcg ccatatatcg acaatcaacg
     2041 ggttcgacaa tccgatgatt cagccgctgt tgtcagatga gcccctgaca gcgctgatgg
     2101 atcattatct tgatacagat gctctggccg acgggctacc gctgtacgtg tcgctgtacc
     2161 ccacagaagg aggcatgcag gatattattg actgcattcg tgctgaactg ggtgccggaa
     2221 ccacgaaaaa cgctgttttt cagcatatcc agagtctgcc ccgcgggcag cagaaagaag
     2281 ccttactggc gtcagccgcg ctgcctctgc tgttccgtcc ccgcgaggtt cagggaacaa
     2341 tgtacggtga tggtgggatg ggcggatggc gaaacaggca ggggaatact cccgtgacgc
     2401 cactggtgga tgccggatgc aatatggtga ttgtgacgca tctgagtgac ggttctttat
     2461 gggatcgtcg ggcttatccg gacaccacaa tccttgaaat ccgtccccgg aaaaggttga
     2521 aacaaatcgg ggacgaaggc aaaagtggcg gtctgctcag ttttacatcg gcacataccg
     2581 acgcctggcg tcagcagggc tacgaggaca cgatgctgac gatggagcat atccggaaac
     2641 cgctggcagc acgtcaggca ctgacccggt cagagacggt attgcagaaa agcctggaga
     2701 taactgaagg tgcagattcg gcactgagaa acgcgatggc ccggattaaa taaagacgct
     2761 ccggagaaac gccaccgcca ctgtggtggt gtttttcctg tctggtatta ttgaaaatat
     2821 tttcagatat atattacctg gatgatggtt aagattaatt tatttaattt tggggagaaa
     2881 actcatgaaa ctgttaaaca ttcttttagc tgttgtttta actgtaagtg caaccgtagc
     2941 acaaacagaa tcggcattgt agacgcgtaa cagtattgct ggcactgata tgttcagtat
     3001 cggtaatgtt tctattaact gcacgattaa tccagaagac aacaatttgc taccacacta
     3061 tgtgtggatt tacggtcata cctccaccca gctaaatcag tacgattctg atattatttt
     3121 tatcatcaac gataaaaagt atccggcccc atctgtcgac ggaacacgaa tgaataagaa
     3181 tgcatgggta tactttatcg atgctatcag tgaagccacg caattcgacg ttttagtaaa
     3241 cggtaagaaa gtcgacagct ataccgcaaa tatcaaaaat gtgaaaaaga ctttgggtaa
     3301 caaattctac gacagctgct ggaacacctg gttccaggaa taattgataa cgtggtgtaa
     3361 aacacctcag tttaatttaa tacaatgagt gaaaatgaat attataaaaa tattttttgt
     3421 ttcagatgtc atatatttta ttaactgaat gattaacagg agaatcagat gaactggcat
     3481 tatgaaaaaa atggtgtacg ttatgataac gtaacagagg aagatattat cgaacgtatc
     3541 cggcgtggtg aactgaatgc atcaacactg gtgtggcaac agggcatgct ggaatggcag
     3601 ccagtttcag ccacatcact ggcggaaata ctgaaacaaa gtgaagttcc cccagccctt
     3661 ccgggtcacc gcattcccgg aggcgttgtc tggacgctgg catttgcccc tttgattggg
     3721 tacatacttg agatgtggac tgcagggctg aacggtatgt catttgaacg ggcttatgat
     3781 gcagtcagcg aaggccagta ctggtttatc tctctgattc tgaatattgc tctgggatat
     3841 ctggatgaac gaaaactccg taaatctggt gttgatactg caacatttgg ctggctggca
     3901 tggttgatcc cggtttatct gtggcgtcgg gcaaaaatcc tcggacaaaa acctgcgtat
     3961 ttctgggtct ggttggttat gctgatactg gtgttattta cggtctgata ttctttatcg
     4021 cagagtttac taaataatca ggttattagt gttcttaaca g
//
LOCUS       DAZPEP010000254         4049 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZPEP010000254 DAZPEP010000000
VERSION     DAZPEP010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.256"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="ACHUT9_004201"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="ACHUT9_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFR1350531.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUT9_004202"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="ACHUT9_004202"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFR1350532.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUT9_004203"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="ACHUT9_004203"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFR1350533.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUT9_004204"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="ACHUT9_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFR1350534.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1142 c          972 g          965 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat cgtcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caacccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacccgat
//
LOCUS       DAZPEP010000255         4043 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZPEP010000255 DAZPEP010000000
VERSION     DAZPEP010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.257"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            926..1642
                     /gene="nanC"
                     /locus_tag="ACHUT9_004205"
     CDS             926..1642
                     /gene="nanC"
                     /locus_tag="ACHUT9_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFR1350535.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            1662..2768
                     /gene="nanM"
                     /locus_tag="ACHUT9_004206"
     CDS             1662..2768
                     /gene="nanM"
                     /locus_tag="ACHUT9_004206"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFR1350536.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2833..3813
                     /locus_tag="ACHUT9_004207"
     CDS             2833..3813
                     /locus_tag="ACHUT9_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFR1350537.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
     gene            3923..4043
                     /locus_tag="ACHUT9_004208"
                     /pseudo
     CDS             3923..4043
                     /locus_tag="ACHUT9_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         1219 a          845 c          915 g         1064 t
ORIGIN      
        1 ggagataagg gggaacgcag gagacgcttt ttcatctaca ttcgtgggta aatatggctt
       61 tggtcactgc gacttaaacc ttcctcaatc acagctattc gccgggcgat acggcaaaca
      121 aactaagtga aacatcatcc tgaaaacacc aacatcaaca agcctctcta gatcgacttc
      181 agaagcgacc agttacaagc cacaaatcag gaaacatatt tcatggtgag aattatgatc
      241 agcctgatgg cgggtaaaca cttgttttca ccacgctcac tgagttagct tgcaaagctt
      301 cccttgttta gaccctaaat aaatttttta taaaacaaac agttaaaaga aatcctttag
      361 tttttgaaga ccacctcaag tgttcgtctg gcttcacatg gcatcttcct cttagaaaaa
      421 gatcgacata ttttgtgaca cgaattgcaa atctcgtttt gttgtatggc ttgcgtgatt
      481 tttgatctgg tataacaggt ataaaggtgc accgcgatag tcaatgagac agggcatctc
      541 gcaatctatg gcaaacatca cttcagttct ttctcatcgg gtgatgaaaa cgcacttcag
      601 tctgaaagga atatgaaaat gagatcaaca gacactctat tttatgactc tgggtaaaat
      661 ggattgagta agtgatatag cttacgaaca ttcaaatcaa ttaaacatca gaagagattt
      721 tatactcagg tatttaatct gattctctgt ttatttaaat aatgtggaaa gagaattttc
      781 acaggagacc ttatacaaaa atataaaata agctacccgt tgccaaagac actataagcc
      841 tggcaaaaaa tattacacaa cataaatgct aattgtttat gcgggctttg tattgctttc
      901 tgtatcctac aaatgagtga aatttatgaa aaaggctaaa atactttctg gcgtattatt
      961 actgtgcttt tcgtcaccat taatttctca ggctgcgaca ctggacgtac gtggtggata
     1021 tcgtagcgga agccatgcct atgagactcg actcaaagtc agtgagggat ggcaaaatgg
     1081 atggtgggca agcatggaaa gtaatacctg gaataccatt catgataata aaaaggaaaa
     1141 tgccgcactc aatgatgttc aggttgaagt taattacgcg attaaacttg atgatcaatg
     1201 gacggtgcgc ccgggaatgt taacgcattt tagcagcaac ggcacacgct acggacccta
     1261 cgtaaaactg tcctgggacg cgacaaaaga tcttaatttt ggcattcgct atcgttacga
     1321 ctggaaagct taccgacaac aagacttatc cggtgatatg tctcgtgata acgttcatcg
     1381 ttgggatgga tatgtcactt accatattaa tagtgatttc accttcgcat ggcaaacgac
     1441 gctatacagc aaacagaatg attatcgcta tgcaaaccac aagaaatggg cgacggaaaa
     1501 tgcctttgtt ctacaatacc atatgacgcc cgatattacg ccatacatag aatatgacta
     1561 ccttgaccgc cagggtgttt acaacggcag agataattta tcggaaaaca gttatcgcat
     1621 tggtgtgtca tttaaactgt agtagacagg agacagtcac aatgaataaa actataatgg
     1681 cgcttgctat catgatggct tcatttgccg caaacgcgtc tgtattaccg gaaactcctg
     1741 tgccatttaa aagtggtacc ggagcaattg ataacgacac tgtctacatt ggtttaggta
     1801 gcgcaggtac agcatggtac aagctagata cacaggccaa agataaaaaa tggactgcgt
     1861 tagctgcatt ccctggtgga ccgagagatc aagcaacctc tgcatttatt gatggcaatc
     1921 tatatgtgtt tggcggcatt ggcaaaaaca gcgagggatt gactcaggta tttaatgacg
     1981 tacacaaata caaccccaaa accaatagct gggttaaatt gatgtcgctc gcgccgatgg
     2041 gcatggcggg tcatgtgact tttgtacaca acggcaaggc ttatgttact ggcggtgtta
     2101 accagaatat cttcaatggc tattttgaag atctcaacga ggctggaaaa gattcaaccg
     2161 ctatagataa aatcaacgcc cactattttg acaaaaaagc agaagattat ttcttcaata
     2221 agtttctgtt gtcttttgat ccctcaacac agcaatggag ttacgctggc gaatcgccct
     2281 ggtacggaac ggctggtgcg gcggttgtga ataaaggtga taaaacctgg cttattaatg
     2341 gcgaagccaa accaggattg cgaacggatg ccgtatttga acttgatttc accggtaata
     2401 atttaaaatg gaataagctt gatcccgtct catcaccaga tggcgtcgct ggcggttttg
     2461 cggggataag caatgattct cttatatttg ccggaggggc cggattcaaa ggttcacgag
     2521 aaaattacca gaacggtaag aactatgcgc atgaaggcct gaaaaaatca tatagcactg
     2581 atattcatct ttggcataac gggaaatggg ataaatcggg tgaattatcg caaggtcggg
     2641 cctacggagt atcattgccc tggaataata gtttattaat tattggcggt gaaactgcag
     2701 gcggcaaagc ggtgacggat tcagttttaa tttctgtgaa ggataataaa gtcacagtac
     2761 aaaactaacg cttcagggcc ccggtaaagg ggccctgcta tcaatttgtc atttgtatta
     2821 cgcgaaaatt atatgaacgc aataatatcg cccgattatt actatgttct taccgttgct
     2881 ggtcagtcta atgccatggc gtatggcgaa ggactgccat taccggacag ggaagatgcg
     2941 cctcattcca gaattaaaca attagcgaga tttgcgcata cgcatcccgg aggcccgcca
     3001 tgtcacttta acgacattat tccactgact cactgcccac acgatgttca ggatatgcag
     3061 ggttatcacc atcctctggc aacgaatcat caaacacagt acggcaccgt tggccaggca
     3121 ctgcatattg cacggaaatt actgcccttc attcctaata atgcagggat tcttatcgtt
     3181 ccgtgttgcc gtggcggatc ggcttttacc gcgggcagcg aagggacata ttcagaacgg
     3241 cacggagcca gccatgatgc ttgtcgttgg ggaacggata ctccgctgta ccaggattta
     3301 gtcagcagaa cgcgagccgc actggcaaaa aatccgcaga acaaattcct cggcgtatgc
     3361 tggatgcaag gcgaatttga cttaatgacc agtgactacg cgtcacaccc tcaacacttt
     3421 aatcatatgg ttgaagcctt tcgtagggat ctaaaacaat accattctca gcttaataat
     3481 attactgacg cgccatggtt ttgcggcgat accacctggt actggaaaga aaatttcccg
     3541 cattcgtatg aagctattta tggcaattat caaaataatg ttttagccaa tattattttc
     3601 gtcgacttcc agcaacaagg tgaaagagga ctgacgaacg cgcctgatga agacccggac
     3661 gatttaagca cgggatatta cggttcagcg taccggtcac cggagaactg gacgacggca
     3721 ctgcgaagca gtcatttcag cacggcagcc cgtcggggga ttatttctga cagatttgta
     3781 gaagcaattt tgcagttttg gcgcgaaagg tgagcgggga gtttattcat gggggatgtc
     3841 cttgttcgtt ctcagagtag acaacaaaaa acaaataaga tcaaggtaat atctcttcac
     3901 ttgcgggctc gaacagcagg caattgccaa tataataatc tggcacaagg gtgaaaaacg
     3961 cgcaccgcat aaaccgttgt tattgcttac agattagccg gatgccttaa tagacatccg
     4021 cgccttttcg attatggctc gga
//
LOCUS       DAZPEP010000256         4041 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZPEP010000256 DAZPEP010000000
VERSION     DAZPEP010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.258"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            376..483
                     /locus_tag="ACHUT9_004209"
     CDS             376..483
                     /locus_tag="ACHUT9_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1350538.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHUT9_004210"
     CDS             complement(570..2249)
                     /gene="bcsG"
                     /locus_tag="ACHUT9_004210"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFR1350539.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHUT9_004211"
     CDS             complement(2246..2437)
                     /gene="bcsF"
                     /locus_tag="ACHUT9_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFR1350540.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHUT9_004212"
     CDS             complement(2434..4005)
                     /gene="bcsE"
                     /locus_tag="ACHUT9_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFR1350541.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1032 a         1011 c         1052 g          946 t
ORIGIN      
        1 cgtgtcatgc gggcgtcagg ctgccgtaat ggcaatttgc gcccggacca ggccgcaggg
       61 gtgaaactct gcggcctttt tcgtaagaaa aatgcaacga ttgcacgcgg ataatattct
      121 gcggaatgcg cagcacagag tgaagacgtg acggattccg gaacagaaaa acagtcgtag
      181 aatcagcggc aagccggaaa ggttccggtg aggcgcaatg ttgcgggggc tttatccctg
      241 gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgca cctgacaaca
      301 tcacgggggc taatcttgaa cccaaacaac tcaagaatag ccgcgaaacg ttggcattac
      361 aacacaggcg gctatatgac gctcgcagaa ctgggcatgg ccttctggca tgatttagcg
      421 gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa taagcggaag
      481 taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt tgcgcccgga tcaggccgca
      541 gaggttaaac tctgcggcct ttttcgtgct tactgcgggt aaggcaccca gtcgccgccg
      601 ttcaggcgaa cgtacggttt atcctggtat tgaataacta ctgcatttga gttctcggag
      661 accggtgctg tttgtggcaa cccactggtg agttttttcc agtcaacatt gtcttcggtg
      721 aaaatcttgc catcaagaac gcgaaccacc agatcggaga tagctaggaa gctgctcggt
      781 tggtcaatga caatcggtgc cccctgatgt ggtgccttca tgccgaagaa tttcacccca
      841 acggggacgt ctgtgataga cgggctaggg atatcacgta ggccagatac ctgcattctg
      901 tcgcccttca gcgcgccgcc gtgttccggc accacgacca ccatcacttt acgacccgat
      961 ttctccagtt cagtaaagaa ggcgtccagt tcatcaaaga atttctgcgc ccgcgctttg
     1021 taatccgctg ttttgctgac ccccggataa tggttgccgt catgcagtgg aagcgtgttg
     1081 tagaacgtgg cactacggct gtttttatct ttttcggtaa cgtccagcca gcggttaagc
     1141 acggcggtat cgtcataaac cggcgaacca tcaaagccca gcaaaataac cggcagattt
     1201 gtttgatcca tcaattcagt ctgcatgccg ccattttcgc gaacttcttt caaaaaaccg
     1261 ccgaactggc cgttatgtcc catcatcagg tgctgggtaa agcccagttt cgaaaggtta
     1321 tcaaacagat agcagtcgtt atttgccggt tgatacagat tagtgtgcga agtctgcccg
     1381 cagctggcgc gcagtaaacg gatcgccgcc gggccactgt aggaggtggc ggagttaaag
     1441 ttcttgaact caatatcgaa atgcgaccac agtggatgcg acatcaaccc ggcggcttct
     1501 atatccgacc aggaaagcga acagatgtta atcaccagta gttcaaatgg ctgagcatca
     1561 gcgggcagcg aagacgggaa ggtcgattta cgtttcgcct ccgcgttata gaaattattc
     1621 agccaggcgt taaggttcgc cgttgttggc ggtgcagttt gtgcgggcat atcacccact
     1681 accgatgcgc cacccgtcgc cgcaacggtt gccgctgcgt taccacccgt cgttgttaca
     1741 gtggtcgtcg gttgtccggc tggccacaag gagaaacttg gtcccgccag ggtaagtacg
     1801 ttcagccata gcagtatggc aaccacaaaa acggtaatgc gaatccattg tgacaggaat
     1861 aaccaggcca ctaataaaac aaaaatggcc ccaatcatct gccagttaat aaagcgtgtg
     1921 acaaggtcga ttaaataatc ggtactgaac cccgccacct gcgaaccctg gctcattatg
     1981 ctttccgggc caggcaacca ggtgtcatgc cagaacaaag caaagccgat cggcagggca
     2041 atccagtggc gcaagcgatg caggctgtag cgcggaatgg gcatcagcag aaacgtggca
     2101 aacaccaaat tgaggagcgg atggaagtta agatatcccg cccacaacag gccgaactta
     2161 accagaaaat agaagttcca gccggaaagg ccgcgccagt attgccagag ggaagaaggc
     2221 atggcggtat tttgcgtaaa ttgagtcatt ttttggttgc cctggctttt tccgtgcggc
     2281 gtaacgtccc ggccggttta acataacgag gtttagcaaa gaataaacgt aaggtgtcgc
     2341 gaatgcgtcg caaagagtgc cgcgccagat agcccagcgg gaaaaatatc agtgcgcaaa
     2401 cgacaataat ttcaatgata tcgctgatgg tcatcatgat gagcgctcca cagcatcatc
     2461 taacagtcgc atgggttctg gtattcgtcg ccagtggcga ccatcgtgct cggcattgat
     2521 gaccggttta gaactttgcg ttaaaggcag cggcatgccc cattgttctg gggcaagcaa
     2581 gcgcatctgc accagctcgg cactgatttg atcatcttca aaccagacca tacggtttga
     2641 gaaaatgtcg ccagtaggca atgggaaaat atgattcaac gcggtatcca gatcgttaat
     2701 ccgacagaat gagagaaaca gcaccagccg attaccgcca atggtcatga tatcgccggt
     2761 acggttaggg cgacacagcg tcagggcttg ttcaacgcgg atacccggta ccggacgtag
     2821 ggcaaccaga acgcctttac cgtgggcagg taatagaggg ttattcatca tgttgttgac
     2881 ggcattacag aacacatccc acttctggaa accacgcagt ttgagcggct gggtcattga
     2941 cagcaaggta gtgatatctt ccggcacata gcgactaaac ttctgccctt gcacgctttc
     3001 gatcatcgtc agacaacggg agagtggcgc attccacgga ataaccatat ttgcaccgca
     3061 ggccaataac aaacgttcat cggtggcgcg caggctagcg gtattttccc gcacgaggat
     3121 tttcatcgca ctaccgcgct ggcgacgcag ggtatgaatg ctgcgggcca gtggctcgat
     3181 ttgcgcattt tgctgtaaag aaaagaccac cgtcgccgcc tgagcggtac gggcttcatt
     3241 gaacaggact tcgttattgt tgaacagttg ccagtgttcc gatagcggcg gcgcaccttc
     3301 cagtacagca acattactca gaatgcgttt ttcgtcgctg cgtggttgga tctccgcctc
     3361 ttcgctttga actaatgtcc agataccatt ttgttgctga acgctaagct gctgacgggc
     3421 gctgacccct ttttcgttgc accagaaggc aatatccagc aaatgttggt caccctgaaa
     3481 acgcaaactg gcaagaccaa aaagtgaacg gtattcctca agcaacaatg aaaattgttt
     3541 atcgttatta tttccgggat taattaccaa aagcgaacaa tggtttaacc tgctccattt
     3601 attcatttta tccaaccatg agcgaagccg ctcggcagga atgttttgcc atgcgttatt
     3661 tgcgcaaaca agaataaaaa ggtaattatg gggatcaata gaacactgca aatcacgggc
     3721 caaatagtat agaccttttt catgatttag cattgaaaat aattttattt tttccggccc
     3781 ttgagaatca tctaattgaa agattttcgc cggatcgctg tccatgctaa tgaccgcgac
     3841 gtgtgcggtt tcagcctggg atgcaattgt ttgattcgcc agactgatag catcttcatg
     3901 gcgatcgacg ttaaaccacc agacgccgcc tgctggcata tgtcgcagct catcccataa
     3961 tgatgagata ccgatagaga atacagggtc cacaatgtcc ctcatgtcac cattttgtct
     4021 gattatcagt ttactagcga a
//
LOCUS       DAZPEP010000257         4012 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZPEP010000257 DAZPEP010000000
VERSION     DAZPEP010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.259"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..657
                     /locus_tag="ACHUT9_004213"
     CDS             <1..657
                     /locus_tag="ACHUT9_004213"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFR1350542.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPH
                     FLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHD
                     VFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDG
                     VVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK
                     "
     gene            complement(602..739)
                     /locus_tag="ACHUT9_004214"
     CDS             complement(602..739)
                     /locus_tag="ACHUT9_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFR1350543.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(712..810)
                     /locus_tag="ACHUT9_004215"
                     /pseudo
     CDS             complement(712..810)
                     /locus_tag="ACHUT9_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(799..1038)
                     /locus_tag="ACHUT9_004216"
                     /pseudo
     CDS             complement(799..1038)
                     /locus_tag="ACHUT9_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905063.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1164..2006
                     /locus_tag="ACHUT9_004217"
     CDS             1164..2006
                     /locus_tag="ACHUT9_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350544.1"
                     /translation="MTGELKIGTVNALRIFNDTFGLIFRRSEDFLHFIPTAEGQGENG
                     NIGPLRPFAINLRTGAISVSHGAKIKGGLAIGATDNALGENSIVLGDNDTGFRQDGDG
                     IISFYSNGSRIGHIDELGLHLYKDIESNGSNFRLKSNYRHHITFANEDGRIRMFLWKD
                     NGGDGVHINNGSDGGGDFIFKTDGGFALGSGAQVASSGDIYGSVWENNWLSTWLHNHV
                     VRDIRLGSIEYKNVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYN
                     VGSV"
     gene            2008..2535
                     /locus_tag="ACHUT9_004218"
     CDS             2008..2535
                     /locus_tag="ACHUT9_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1350545.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2564..3097)
                     /locus_tag="ACHUT9_004219"
     CDS             complement(2564..3097)
                     /locus_tag="ACHUT9_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1350546.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(3100..4012)
                     /locus_tag="ACHUT9_004220"
                     /pseudo
     CDS             complement(3100..4012)
                     /locus_tag="ACHUT9_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230582.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber protein"
BASE COUNT         1144 a          884 c          861 g         1123 t
ORIGIN      
        1 atgaaaaaaa acacagatga tggggctaaa atttacacac cacttaccct aaagctttat
       61 gactggtggg ttttgggagt atcaaatcgg cttgcatggg gatgtcctac aaaggaacac
      121 cttcttccac actttctgga acatttaggt aacaaccatc tggatattgg tgttggaact
      181 gggttttacc ttactcacgt acctgagagt agtctgatat ctttaatgga tttgaacgaa
      241 gctagcctga acgcggcatc tacaagggct ggggaatcaa aaattaaaca taaaattagc
      301 catgatgttt ttgaacctta tcccgcggcg ttacatggtc aatttgattc catttccatg
      361 ttttaccttc ttcactgcct gcctggaaat atatctacaa aaagctgtgt aatacgcaat
      421 gcggcgcagg ccttaactga tgatggaact ctatacggag ccacaattct tggcgatgga
      481 gttgtgcaca atagcttcgg tcaaaaactg atgcgcattt acaatcagaa aggcatcttt
      541 tcaaacacaa aagattccga agaaggctta acacatatac tctcagagca tttcgagaat
      601 gttaaaacca aggttcaagg tactgtagta atgttttccg cttcagggaa aaaatagcat
      661 ccaaccgcag cacgttcttg cttaagacgt gctgcggcat aatcccaatg attactccct
      721 gacagggttc gtaggccact caatatcagg tgcagttgat gtatcaacac ggttcagcaa
      781 cacccgatac tttttctagg cttccaccac cagcacgaca agatgccgca tacagtgacc
      841 cagtcagtcc agttttcaga caaccagtgc gtcacctttt tgaaggcgct ttaaagcacg
      901 ttttaatcca ggtcggcctg tccttgttcc gcttaattta tcttcaaata tttgttcata
      961 tcctgcacaa acaagagcgt ttcgttgcag gtctgtattc tggtcatttg ttgataccct
     1021 tacatagcca atcagcacgc tgaatctccc gtccaaaagc acaaatcatg ccatgcaggc
     1081 cagaaaccgc cattatctaa aacctcggtt tacaggaaac ggtaaacaag gctggtaacg
     1141 ccgttcagcg ttccggcgat aaaatgaccg gagaactgaa aattggcacg gtgaatgcgc
     1201 tgcgaatttt caatgatacc ttcggtctaa ttttccgtcg ttcagaagat tttcttcatt
     1261 tcattccgac ggctgaagga caaggcgaaa acggtaatat cggcccatta aggccattcg
     1321 ctataaatct gagaacaggt gctatatctg tcagccacgg ggccaaaatt aaaggaggtc
     1381 tagctattgg agctactgat aacgcactgg gtgaaaactc cattgttctg ggagataacg
     1441 acaccggatt taggcaagat ggagatggta ttattagctt ctattcaaat ggttcgcgca
     1501 tcggacatat tgatgagtta ggattacatc tttataaaga tattgaatct aatggcagca
     1561 attttagatt aaaaagtaat taccgccacc acattacatt cgccaacgaa gacggaagaa
     1621 ttcgtatgtt tttgtggaaa gataacggtg gtgatggtgt tcatattaat aacggttcag
     1681 atggtggtgg tgatttcatt tttaaaacag atgggggatt tgcgctggga agtggtgcgc
     1741 aagttgcgtc tagtggcgat atttatggtt cagtgtggga aaacaactgg ttaagcacat
     1801 ggctgcataa tcatgtcgtt cgggatattc gtcttggcag cattgaatat aaaaacgtat
     1861 ggcgcgacta cggctttggc gatgcgtcag gttatgtttt aacagccgca attaacagca
     1921 atgcggatga tattgtcgac actgttgcca gaaggccaat tcagaaattg attgggggaa
     1981 tatggtacaa cgtggggagt gtttaagatg atgcacctga aaaatattaa ggccgggaat
     2041 ccgaaaacac cggaacaata ccagctaact aaaaaagcgg gcgtggtatg gctcttttgc
     2101 gaagatggca aaaactggta tgaggaacaa aagaattttc agccagacac cataaaaatt
     2161 gcttacgatg agaacaacat catcgttgct gtcagtaaag acgtttcaac tattaatcct
     2221 gaagggctaa gcatagttga agttcctgat attacggcaa atcgtcacgc agacaataac
     2281 ggaaaatgga tgtttctgga tggtaaagta gtaaagaggg aatatacaga acaggaactg
     2341 caacaacagg ccgaatcaca aaaggccgca ttgctttccg aagctgaatc agtcatccag
     2401 ccgctggaac gcgctgtcag gctgaatatg gcaacagacg aggaacgcac acgactggaa
     2461 gcatgggaac gctacagtgt tctggtcagc cgtgtggata cggcaaatcc tgaatggcca
     2521 caaaagcctg aataaaaatt aaggcccgat agcgggcctt ctctcattct ggttgttcgg
     2581 gaaacgttac tggcaggctg gaggtgtctg tggattcaac cttctgcgca tagagcatcc
     2641 actcggttaa tttttgttta ttctcatcgg aaatgatgcc cagctgtagc tgagagtccc
     2701 acagctgggt tttatccctg acgagctgca gcaggctctg cttttcattt tccgcttgtt
     2761 gcctttgctc ttcctcggta taagttcgct ttatcaccgc accatctttg aacatccagt
     2821 tgcccgaaat atcagcccgg cgatttgctg taatatcagg caactcaacg acacttaaac
     2881 cttctggatt aattgctgat acatccttgt ctatagcaat aattattccc tcaactgtgt
     2941 aagccagttt tattgtatcc tcctgaaagt tttttaattc ctcatgccaa ttttttccat
     3001 cttcggacca taaccagata acatcaaaat tctttgttag ctggtattgt tcaacggttt
     3061 ttggattacc tgcgacaatg ttttttaaat gctgcatgat tatacctgcg atacgttata
     3121 ccatgttccg ttaattaact tttgtattgg tctgaatact gccggatcat caccatcgac
     3181 ttcaccgata ataccaagcc ccgtgattac atggcctgat ttttcataca tcacgccttt
     3241 cttcatggtc taaacaactc gtgtgccaag tctgacatct ctcacatagc gggaatcaaa
     3301 gttaccgtaa tccgagggat taacacgtcc cgtaatattt atggttttat tactttgaat
     3361 gcttccggag acaaagcgca taacatggac gttattagca taaacatcca gattaccatc
     3421 gcctttttgt ttaaagcccg tgtcattatc acccaataca atcgaattac cgccaagagc
     3481 actggatgtt ccgataccca gtgcaccatt cagttgacca ccagataacg gcagtgcacc
     3541 aacatctcct gctgaaggtt ttctggtggt gtaaaattcg gaccagtcgg cttcaaaacc
     3601 ataaccatca cgggctgaac gataaaaaat accgccattt ttataattaa tccgaaactg
     3661 agctgcagga caacttcctt ctcccatata aaaatgaata attaacgttg atgcaccact
     3721 aatagttgcg ttataggctc cgctactcca gttccatcca actgctttat cattcgcaac
     3781 ggtgcttcct gttttcccta aggcaaacgc accaacatga cttgctttta atgtgatatc
     3841 ggaggaacca tcaaaagcca cattgcttat tttcctggca gtttttaatt ttgcagctgt
     3901 agaagcattg ccggatagtt caccagaaag gccaccgctg aatgtttgtc gattagtcca
     3961 ggtattcgct gtactgagta acggtatttt ctccccgctt gtgccgagtt ct
//
LOCUS       DAZPEP010000258         3959 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZPEP010000258 DAZPEP010000000
VERSION     DAZPEP010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.260"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..242)
                     /locus_tag="ACHUT9_004221"
     CDS             complement(<1..242)
                     /locus_tag="ACHUT9_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1350547.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHUT9_004222"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHUT9_004222"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFR1350548.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHUT9_004223"
     CDS             2255..3904
                     /locus_tag="ACHUT9_004223"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFR1350549.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT         1031 a         1001 c          927 g         1000 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtaacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccacc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 tagacgccga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaagatt tcgacgccat ttcaatcggg ttcaacggcg gtccaccagc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tccgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gaagcgcggt ataacgcgtt ggcggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggcg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaaacctaa aaccttatta ttttcactga cagcggatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagtgagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc ccggacagta aaacctttcc cgactgcgca cctaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctac tggcgctgcc gcagtcatat attgtcccag gcggccgttt
     2761 tagcgaaacc tactattggg actcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttactggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggggcggcc tccggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaacggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagg
//
LOCUS       DAZPEP010000259         3946 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZPEP010000259 DAZPEP010000000
VERSION     DAZPEP010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.261"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            30..968
                     /locus_tag="ACHUT9_004224"
     CDS             30..968
                     /locus_tag="ACHUT9_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFR1350550.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDNPPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            1024..2313
                     /locus_tag="ACHUT9_004225"
                     /pseudo
     CDS             1024..2313
                     /locus_tag="ACHUT9_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            2310..2603
                     /locus_tag="ACHUT9_004226"
     CDS             2310..2603
                     /locus_tag="ACHUT9_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFR1350551.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            2768..3946
                     /gene="fadK"
                     /locus_tag="ACHUT9_004227"
                     /pseudo
     CDS             2768..3946
                     /gene="fadK"
                     /locus_tag="ACHUT9_004227"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
BASE COUNT          929 a         1003 c         1088 g          926 t
ORIGIN      
        1 cctgaactga agcctacgga gaagaaacga tgagtcaatt aaacagcgtc tgggtcttta
       61 gcgataatcc tgaacgttat gctgaactgt ttggcggcgc gcagcaatgg ggccaacagg
      121 tgtatgccat tgtacaaaat accgaccagg cgcaggcagt tatgccttat ggtccaaaat
      181 gtatttatgt tcttgagcaa aatgatgcgc tgcaacgtac tgaaaattac gccgaaagca
      241 ttgctgccct gctgaaagat aacccccccg ctatgttgct gctggccgcg acgaaacgtg
      301 gtaaagcgct ggcagcacgg ttaagtgtgc aactgaatgc ggcgctggtg aacgatgcca
      361 cggcagtgga tattgtcgat ggtcacattt gcgccgaaca ccggatgtat ggcgggttag
      421 cgttcgctca ggaaaagatc aacagcccgc tggcgatcat tacccttgca cccggtgttc
      481 aggaaccgtg caccagtgat acctctcatc agtgcccgac agaaacggta ccttatgttg
      541 ccccgcgtca tgaaattctc tgtcgcgaac gccgtgcgaa agcggcaagc agcgtggacc
      601 tgagcaaagc aaaacgtgtg gttggcgtcg gtcgtggtct ggcagcgcag gatgacctga
      661 aaatggtcca cgaactggcg gcggtgctga atgctgaagt cggctgttca cgtccaattg
      721 ccgaaggcga gaactggatg gagcgtgaac gttatatcgg tgtctccggc gtgttgctga
      781 aatccgatct ctacctgacg ctggggatct ccgggcagat ccagcatatg gttggcggca
      841 acggcgcaaa agtgattgtc gccatcaata aagataaaaa tgcgccaatc ttcaattatg
      901 ccgactacgg tctggtgggc gatatctaca aagtcgtccc tgccctgatt agccagttga
      961 gccgctaatt cccctttcaa ccacatctcc cgctgtaaac gcagcgggaa gggagcataa
     1021 cgcatgtcgg atgacaaatt tgatgccatt gtggtcggtg cgggcgttgc tggtagcgtt
     1081 gccgcactgg tcatggcgcg agccgggctg gatgtcctgg tgatagaacg cggcgacagt
     1141 gccggatgta aaaacatgac cggcgggcgt ctttatgccc acacacttga agcaatcatt
     1201 ccaggctttg cagtatcagc gccggtagaa cgcaaggtca cacgcgagaa aatctccttc
     1261 ttaaccgaag aaagcgccgt taccctcgat tttcaccgcg agcagccaga tgttccgcaa
     1321 cacgcatctt ataccgtatt gcgtaatcgt ctggacccgt ggttgatgga acaagccgag
     1381 caggcgggcg cgcagtttat cccaggcgtt cgcgtcgatg cgctggttcg tgaaggaaac
     1441 aaggtaactg gcgtgcaggc tggggatgat attctcgaag cgaatgtggt gattctggct
     1501 gatggcgtta actcgatgct tggccgctcg ctgggaatgg ttcccgcttc cgatccgcat
     1561 cattacgctg ttggtgttaa agaggttatt ggcctcacac cagaacagat caacgatcgc
     1621 tttaatatta cgggcgagga aggtgccgcc tagctgtttg ccggttcccc ttctgacggc
     1681 ctgatgggtg gcggattcct ctataccaac aacgattccg tatcgttggg gctggtttgt
     1741 ggattgggtg atatcgccca tgcgcaaaaa agcgtgccgc aaatgctgga agattttaaa
     1801 caacaccccg ccattcgccc actaattagc ggcggcaaac tgcttgaata ttccgcgcat
     1861 atggtgccgg aaggcggtct ggcgatggtg ccgcaactgg ttaacgaggg cgtgatgatc
     1921 gttggtgacg ccgcaggctt ctgtctgaat ttgggtttta cggtccgcgg catggattta
     1981 gccattgcat cggctcaggc tgccgccaca acggtgatcg ccgccaaaga acgcgaggat
     2041 ttctccgcca gcagtctggc gcaatacaag cgtgagctgg aacaaagctg cgtcatgcgt
     2101 gatatgcagc attttcgcaa gatcccggcg ctgatggaaa acccgcgcct gtttagccaa
     2161 tacccacgaa tggtagccga catcatgaac gagatgttca ccatcgacgg caaaccaaac
     2221 cagccggtac gaaaaatgat catgggacat gcgaagaaaa ttgggctgat caacttgctg
     2281 aaagatggca ttaagggagc aaccgcgcta tgagccagaa cgctacggtt aacgtcgaca
     2341 tcaaattagg cgtcaataaa ttccatgttg atgagggcca cccgcatatc attctggcgg
     2401 caaatcccga catcaatgaa ttccgtaaat taatgaaagc ctgccctgcc ggactttata
     2461 agcaggatga cgccggcaac attcattttg attctgccgg ttgtctggag tgcggcacct
     2521 gtcgggtgct gtgcggtaac actattctcg aacagtggca atatcccgca ggcaccttcg
     2581 gtattgattt tcgctacggc taatcatgca tcccacaggc ccgcatctcg ggcctgatgt
     2641 tctgtttcga gagtccaaca tgaaagtgac attaacgttc aacgaacaac gtcgtgcggc
     2701 gtatcgtcag caagggctat ggggcgatgc ttcgctggcc gattactggc agcagaccgc
     2761 tcgtgcgatg ccagacaaaa ttgccgtggt cgataatcat ggtgcatcgt acacctatag
     2821 cgcgctcgat cacgccgcga gctgtctggc aaactggatg ttagcgaagg gtattgaatc
     2881 aggcgatcgc atcgcatttc aactgccagg ctggtgtgaa tttaccgtta tctatcttgc
     2941 ctgcctgaaa accggtgcgg tttccgtgcc gctgttgcct tcctggcggg aagcagaact
     3001 ggtgtgggtg ctcaataagt gtcaggcaaa aatattcttt gcaccgacgt tgtttaaaca
     3061 aacgcgtccg gtagatttaa tcctgccgct gcaaaatcag cttccacaac tacaacaaat
     3121 tgtcggcgtg gacaaactgg ctcccgccac ctcttccctc tcattaagtc agattatcgc
     3181 cgacaatatc ccactgacca cggcgataac gacccacggc gatgaattag ctgcggtgct
     3241 gtttacctcc ggaaccgagg gtctgccaaa gggcgtaatg ctaacgcata acaatattct
     3301 cgccagtgag cgggcttatt gcgcgcggct gaatctgacc tggcaggatg tctttatgat
     3361 gcctgcgcca cttggtcacg caacgggatt tctgcatggc gtaacagcac catttttaat
     3421 tggtgctcgc agcgtgttgt tagatatttt cactcctgat gcatgtctcg cgctgcttga
     3481 gcagcagcgt tgcacctgta tgctcggcgc aacgccgttt gtctatgatc ttttgaattt
     3541 actagagaaa caacccgcag acctttcagc gctgcgcttc tttctttgcg gcggtaccac
     3601 aatccccaaa aaagtggcgc gcgaatgcca gcagcgctgg cattaaatta ttaagtgttt
     3661 atggttccac agaaagttcg ccgcatgcgg tggtgaatct cgatgatcct ttgtcgcgct
     3721 ttatgcacac cgatggttac gctgccgcag gtgtagagat taaagtggtc gatgacgcac
     3781 gcaagacctt accgccaggt tgcgaaggtg aagaagcctc gcgtggcccc aatgtgttta
     3841 tggggtattt tgatgaacct gaattaaccg cccgtgcgct ggatgaagaa ggctggtatt
     3901 acagcggcga tctctgccgt atggatgagg ctggctatat aaaaat
//
LOCUS       DAZPEP010000260         3908 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZPEP010000260 DAZPEP010000000
VERSION     DAZPEP010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.262"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            24..590
                     /locus_tag="ACHUT9_004228"
     CDS             24..590
                     /locus_tag="ACHUT9_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFR1350552.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="ACHUT9_004229"
     CDS             594..1169
                     /locus_tag="ACHUT9_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFR1350553.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="ACHUT9_004230"
     CDS             complement(1211..2263)
                     /locus_tag="ACHUT9_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFR1350554.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUT9_004231"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="ACHUT9_004231"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFR1350555.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3765
                     /locus_tag="ACHUT9_004232"
                     /pseudo
     CDS             3580..3765
                     /locus_tag="ACHUT9_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
BASE COUNT          971 a          987 c          952 g          998 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acggcgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta tgcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAZPEP010000261         3840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZPEP010000261 DAZPEP010000000
VERSION     DAZPEP010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.263"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            69..1010
                     /locus_tag="ACHUT9_004233"
                     /pseudo
     CDS             69..1010
                     /locus_tag="ACHUT9_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            1010..1108
                     /locus_tag="ACHUT9_004234"
                     /pseudo
     CDS             1010..1108
                     /locus_tag="ACHUT9_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            1108..2429
                     /gene="hipA"
                     /locus_tag="ACHUT9_004235"
                     /pseudo
     CDS             1108..2429
                     /gene="hipA"
                     /locus_tag="ACHUT9_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2613..2714
                     /locus_tag="ACHUT9_004236"
                     /pseudo
     CDS             2613..2714
                     /locus_tag="ACHUT9_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            3075..3612
                     /locus_tag="ACHUT9_004237"
                     /pseudo
     CDS             3075..3612
                     /locus_tag="ACHUT9_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            3654..>3840
                     /locus_tag="ACHUT9_004238"
     CDS             3654..>3840
                     /locus_tag="ACHUT9_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350556.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAF"
BASE COUNT         1070 a          790 c          936 g         1044 t
ORIGIN      
        1 taggtgggca ttgcacatat ttccgacgaa tagatcacaa tttatgctat tttgattttc
       61 acggttgcac atcaacagtt aatttgcgta aatcctatgg actgacaacg aaattcagta
      121 gattgacgct gggtgttttg ctggcactaa gcggttcagc gtctggtgcg agtctggaag
      181 ttgataatgg tcagattacc aatattgata ctgatgttgc ttatgatgcc tacctggttg
      241 gctggtatgg cactggagtt cttaatattt tggctgacgg taatgcctac ttaaccacta
      301 ttactaccag cgtcattggc gctaatgagg attcggaggg taccgttaat gttttgggtg
      361 gcacctggcg attgtatgat agcggaaata atgcaaggcc tttaaatgtg ggtcaatccg
      421 gaacggggac gctgaatatt aaacagaaag gtcacgttga tggaggctat ttaagactag
      481 gttcctcgac aggaggcgtc gggacggtca atgttgaggg agaagactct gttctgacga
      541 ccgaattatt cgaaataggg agttacggta cgggctcatt aaatattacg gataagggtt
      601 acgtcacgag ttcaatagtc gccattttag gatatcaagc gaacagtaat ggtaaggtta
      661 tcgttgaaaa gggtggcgag tggctaataa aaaataatga ttcctcaatt gaatttcaaa
      721 ttggtaatca aggagctggg gaggcgacta ttcgcgaggg tgggctgatt acggctgaaa
      781 atacgattat cggtggcaat gccaccggta tcggaaccct gaatgtgcag gatcaagact
      841 ctgtcatcac ggtacgcaga ctctataatg ggtatttcgg taatggcaca gtcaatattt
      901 ccaataatgg tctgattaat aacaaagaat attcattggt gggcgttcag gacggttccc
      961 acggtgtcgt caacgtgacc gataaagggc attggagttt cctcggaact tttttaagat
     1021 tttacagtcg cttgaactct caatgacgct atgcgacgcg aaaaatgcct cgccagaagc
     1081 aacagaacag caagatctgg agtggtaatg cctaaacttg tcacttggat gaacaaccag
     1141 cgggtaggcg agttaacgaa gttagccaac ggcgcgcaca cctttaagta tgcaccggag
     1201 tggttagcca gccgttatgc cagaccgttg tcactttcgc tgccattgca gagggggaat
     1261 atcacctctg atgccgtatt taacttcttc gataacctgt tacccgatag cccgattgta
     1321 cgtgaccgga tcgttaaacg ttatcatgcc aaatccagac aaccgtttga tttgttgtca
     1381 gaaatagggc gagacagcgt tggtgccgtg acgttaatac ccgaagacga aaccgtaacg
     1441 cgtccgataa tggcatggga aaagcttact gaagccagac ttgaagaagt attaacggct
     1501 tataaagcag atatcccgct aggcatgatt agagaagaaa atgactttcg catctcggtt
     1561 gctggcgcac aggagaagac agcactgctc agaataggca atgactggtg cattccgaaa
     1621 ggaataacgc cgacgacgca catcattaaa ttaccgattg gcgaaatcag gcagcccaat
     1681 gcgacgctcg atctcagcca aagcgttgat aatgagtatt actgtctgct gctggcgaaa
     1741 gaacttgggt tgaatgttcc ggacgcagaa atcattaaag cgggaagggt gcgcgcgtta
     1801 gcggtcgaac gttttgacag gcgttggaat actgagcgaa cggttttact tcgcttgcca
     1861 caggaggata tgtgtcagac attcggttta ccttcatcgg tgaaatatga atcagatgga
     1921 ggcccaggca tcgcgcggat catggcgttt ttgatggggt ccagcgaggc gctgaaagat
     1981 cgctatgatt ttatgaagtt tcaagtgttc cagtggttga ttggcgcaac ggatggccat
     2041 gcaaaaaact tctccgtatt tattcaggct ggcggcagtt atcgactcac gccattttac
     2101 gacatcattt cagcatttcc ggtccttggc ggtacgggaa taacatcagc gatctcaaac
     2161 tggcaatggg gcttaacgca tccaaaggca aaaaaacggc aatcgataaa atttatccgc
     2221 gacatttttt ggcgacagca aaggtgctga gattcccgga agtgcagatg catgaaatcc
     2281 taagtgactt tgccagaatg attccggcag cactggataa cgtgaagact tcattaccga
     2341 cagattttcc ggagaacgtg gtgacggcag ttgaaaccaa tgtgttgagg ttgcatggac
     2401 ggttaagccg agaatacggt agtaagtgag atatggggtt tggtcgttgt taatgagcaa
     2461 tcatgaccgg ccaacgactg gattctccct tggtttatgt ttgtgcgcat aatgtatatt
     2521 atgttaaatc atgtctgagt gatgcgaagt gcttttctgg tagtcgttat tcgttcagat
     2581 taggtgtcct tgtgatttaa agatgttatc gtggcacgaa tatactgcca ttccaggctc
     2641 gttatattgc tctcgggcag tccgtagctg gtactgcaaa cgctgatgcg accttcaaag
     2701 ttcaatatct ataattcaat cttaaggtgg ctatacgtca tctgaaattg aaacagggtg
     2761 tgtgtggcat ggctacgcac acccgagcaa ttcaccaatg tgaatctcat tattaatgag
     2821 gagtggtttg tatttggagc gctatgaaaa atcacataat atctaaacta tcaataaaca
     2881 acagtagtta aacttactgt tatttaaaat atttactttg tagcaaggtt ttcctattaa
     2941 aagagaacca acatttatta caaacgcatt aagtctggaa acccggcgac tcgccaatgt
     3001 attagcaaca ttaatcgatt gtctgatgta acggttttcc cgcttatcct cttaacacat
     3061 ggagaatgca tcagatgcaa actacgcgta ccccctactc tatttccttt atggcgacag
     3121 tgttactttt attgctcttt gcttgccata gcaccgttgc caacgcagct gtcgcactgg
     3181 aatacgctgc ggataatcaa cgctactaat catcagttac caggcgaccg tgaaagcctg
     3241 ttctgggtga atgttaaagc tattcctgct atggaaaaag accagaagaa tgagaataca
     3301 ttacagctgg caattattag ccgcataaaa atgttttatc gccccacgaa cctggcaatg
     3361 gcacctgaag aagcgcctgc aatgcttcgg tttcgtcgta gcggtagcaa actcactctt
     3421 attaatccaa cgccctattt cattaccgta acaaatatga aagcaggaaa tagcaatctc
     3481 ccaaacacca tggtgccgcc aaaaggtgaa gtatcggtgg atatcccgca tgctgcaacc
     3541 ggggatatca gttttcagac gatcaatgat tacggtgcat taactccgcg tattaaggca
     3601 accatgcagt aagccagtga cctaattaat aataaactca atcggaatta ataatgacag
     3661 cctttcgtgc cgcatttaag gcatatcgta tgcatcaggt gttaatactg cctcgctttg
     3721 cacggcttac cattgccctt ggcttagcaa ctgcggtatt tcccgttgac gcagagtatt
     3781 attttaatcc acggttttta agcaatgatc ttgctgaatc tgtcgattta tccgccttta
//
LOCUS       DAZPEP010000262         3834 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZPEP010000262 DAZPEP010000000
VERSION     DAZPEP010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.264"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..266)
                     /locus_tag="ACHUT9_004239"
     CDS             complement(<1..266)
                     /locus_tag="ACHUT9_004239"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFR1350557.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="ACHUT9_004240"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="ACHUT9_004240"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFR1350558.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="ACHUT9_004241"
                     /pseudo
     CDS             complement(726..998)
                     /locus_tag="ACHUT9_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHUT9_004242"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="ACHUT9_004242"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFR1350559.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3734)
                     /locus_tag="ACHUT9_004243"
     CDS             complement(2694..3734)
                     /locus_tag="ACHUT9_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFR1350560.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMTSISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
BASE COUNT         1000 a          932 c          916 g          986 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt acagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcca cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaagg cacaataact ttcgctatct tcgatacccc attgattctc taaagactcg
     3241 cgtcttttac tgatgatatc gatcgagtca aaaggaagca catgatattg gaaggtatct
     3301 ttaccaggtt caggctttcg cggccagaac tctagcgttt cagaccattg cttatgatag
     3361 aatcgataag gtgcgatcaa ttgtagcgcc tgtaacttct cgatactgag cggctcaata
     3421 cctttagcct gataataatg cagttgttct ttttttgctt taaaaccggc ccgaacaata
     3481 agccccatca taattaatag ataaagaaaa gagcatcccg cggtaatcag gcctctttca
     3541 ttcaaaccgt tggatgttat cgctgcgaac acaaacatta cagcgacaac acatgttaaa
     3601 taaaaccccc acttacaaag cagcatggcc ttattttctt taatcatccg ttcaaaatta
     3661 ctattaaata tttcccagcc attaaaagaa tacttctcgc tcccaggatg gttttgtaat
     3721 aaaacttttt tcatcacatt ccctgttatt acatacttta gaggcgataa ttttatccat
     3781 gcaaaaaaaa tatccaacga attttcttga tccgctggat atttcagaat atga
//
LOCUS       DAZPEP010000263         3827 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZPEP010000263 DAZPEP010000000
VERSION     DAZPEP010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3827
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.265"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..196
                     /locus_tag="ACHUT9_004244"
     CDS             <1..196
                     /locus_tag="ACHUT9_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350561.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            207..374
                     /locus_tag="ACHUT9_004245"
     CDS             207..374
                     /locus_tag="ACHUT9_004245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350562.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            371..715
                     /locus_tag="ACHUT9_004246"
     CDS             371..715
                     /locus_tag="ACHUT9_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350563.1"
                     /translation="MTTFTREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLTWQLRKAKPDCKLPDKAMSYLE
                     RNGLISVEDILR"
     gene            1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUT9_004247"
     CDS             1007..1162
                     /gene="hokD"
                     /locus_tag="ACHUT9_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1350564.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1383..1671
                     /locus_tag="ACHUT9_004248"
                     /pseudo
     CDS             1383..1671
                     /locus_tag="ACHUT9_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1710..1988
                     /locus_tag="ACHUT9_004249"
     CDS             1710..1988
                     /locus_tag="ACHUT9_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350565.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1990..3048
                     /locus_tag="ACHUT9_004250"
     CDS             1990..3048
                     /locus_tag="ACHUT9_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFR1350566.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMSAFHNGRILVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIALLPHVHQQQNPPQEKTVVSIAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            3049..3429
                     /locus_tag="ACHUT9_004251"
     CDS             3049..3429
                     /locus_tag="ACHUT9_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1350567.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3426..>3827
                     /locus_tag="ACHUT9_004252"
     CDS             3426..>3827
                     /locus_tag="ACHUT9_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1350568.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT         1034 a          852 c         1046 g          895 t
ORIGIN      
        1 atcaccgaaa aaaattggct tttacaccat tggcgataaa agcatttacc tgtatgacct
       61 acgccgcatg gatgaaatca tggaggctct tgataatcgt tcgtcgatgg attggtgtgt
      121 tgctgttcat gatatgaatg cagggtttga tgaaaagatt ttgttcccct catcagttga
      181 aagcactgcg ggttaaggag taacacatga ccactattac caaagaacgt attgaattgt
      241 tcattaaaaa cccgcttgaa aacgggctta cccgtggtga acaaatggaa ctggcacgga
      301 ttgcgctggc atcgctggaa gcagagccgg ttgtgttctg gtttgaaaaa tatcaagaag
      361 gggctacggc atgacgactt ttaccagaga gcagttaata gctcacgcag aggagactat
      421 tgaagcacag agactgtgca taccgggcac aatcgaccat gacatcatcc gcacatataa
      481 gatggatatt gctgttctgg aaatcgcact ggtatcgctg gcagcagagc cagccggtaa
      541 attgcatgaa tacaaaccag tgggatatca gcgtctggtc gatgagttaa ccatgctggt
      601 aaagcagtta acctggcaac tgagaaaagc gaagccagac tgcaaattac cggataaggc
      661 gatgagttat ctggagcgga acggactgat aagcgtggag gatattttac gatgacctgg
      721 cctgaagcat taacaacggt aggaattgcg atggcggtgg cgctggtggt gtattcgatt
      781 tgccgctggg gataaaaacg gtttgcggga aaaggagagt taagtagaat tgcagcgggt
      841 gcttgaggct atctgtctca ggcatgaaca ccaaaaggca gatagagaaa agccccagtt
      901 aacattacgc gtccggcaag acgcttaaca ttaatctgag gctcaatcta tgaacggcaa
      961 atctaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     1021 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc atagtgacgg cactggtaac
     1081 gaggaaagac ctctgcgagg tacgaattcg aaccggccag acggaggtcg ctgtcttcac
     1141 agcttacgaa cctgaggagt aagagaccag gcgggggaga aatccctcgc cacctctgat
     1201 gggtcaggca tcctcaatgc acccacactt aacccgcttc ggcgggtttt tgtttttatt
     1261 ttcaacgcgt ttgaagtttt agatggtgcc ggaatagaat caaaaatact taagtagcgc
     1321 gcagggagaa gagggatgga ccccgaacag gggagtgcta tttatctgga aggattctgt
     1381 tgatgaaaat cgaagaatta cgtgaaattt ttagtgaaga tggcctctat actgtgcgcg
     1441 ttgagaatgg cgctattgtc agccactgcc gtattaaatg tttacagtct caacaaagga
     1501 agagtggagc tgcgttaatt cattttgtgg atgggcttgt gacggatggt tttatttcgc
     1561 gtgcaaatga atttgtcaca tcgttgccgt ctctgaaaga agctgggatt aaggctggtt
     1621 tttctgcttt tgaagatgag tgaattcatc tacaattcag cgcagggctg aacccctgtt
     1681 gagtaacact gtgccaccgg agaaagccga tggcgcaaaa ttccagacta cacaattctg
     1741 ataattcagc cgtctttgcc agcaggcacg ggcggcgctc tcacgcattt aaatctgatt
     1801 ggtaccagca tcccccatgc actgaagaac aggccgaatg gctcattcag tgttaccgca
     1861 ggcgcggatg cgaggttaaa aaagccctta gcctcgacta ccgtcactgg ataatctccg
     1921 tcaggctccc ttactccgaa cggccaacgc gtccgtcccg cacattccag caacggatct
     1981 ggaggtaatg tgcgggtatt acttcgacct gttctggtac cggaactcgg tctggttatc
     2041 gttaagccag gccgtgaatc aatgtcagca ttccataacg gcagaatact ggtggagccg
     2101 gaaccaaaaa gcatgcgtaa tctgccgtcc ggggtcgttc ctgccgctcg ccagccgctg
     2161 gtggaagaca aaacattgct gccgtttttc agtaacgcac gggtgattcg tgctgctggt
     2221 ggtgctggtg cattgtctga ctggctgttg cgccatatta aatcctgcca gtggccacac
     2281 ggcgattatc atcacagcga aaccgtcatt caccgttatg gtaccggcgc aatggtgttg
     2341 tgctggcact gcgacaacca gctgcgtgac cagacatccg aatcactcga gcaacttgct
     2401 catcaaaacc tgtcagcatg gatgattgac gtcatcggtc acgcaataag cggtacgcag
     2461 gagcgtgaat tatctctggc tgaattatcc tggtgggcgg tccgcaatca ggtggcggac
     2521 gcgctaccgg aagcggtatt acgtcgttcg ctggggttgc gtgcggaaaa aattcgctct
     2581 gtgtacagtg aaagcgacat cataccggga gagcagacag ccaccagcat actgaagcag
     2641 cgcacaaaaa atattgcgtt actgcctcac gtccaccagc aacagaaccc accacaggaa
     2701 aagacggtgg tcagcattgc cgttgatccg gagtcaccgg ctcagtatct ccagcgccag
     2761 aaatcacaac gggaagagat gcctgtatac acgcgctggg taaaaacgca gaaatgcatg
     2821 acgtgtggca atcaggcaga tgatccgcat cacatcattg gtcatggact gggagggatg
     2881 ggaacaaagg ctgatgattt gtttgttatt ccgttatgcc gtaaatgccg taacgaacta
     2941 catgccggag taaaagattt tgaagagaaa cacggcagtc agctgttgtt gctgattcgt
     3001 tttttaatgc acgcgagaaa ttcgggtgtt ctgaagtgga aagcataaat gaccgagcgc
     3061 atagaatttg ttttacctta cccgccgacg gtgaacactt actggcgtcg tcgtggcagc
     3121 acatattttg tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc
     3181 cagcagcggc tgaaattaag cctgtccgga aggttggcaa taaaaattat tgcagaacca
     3241 ccggataagc gccgccgtga cctggacaat attctgaaag cgccgctgga tgcgctgacg
     3301 catgcggggt tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag
     3361 ccagtatctg gtggacgtct gggggtgaag atttatccca taatgcttga agggcaggtc
     3421 aaaaaatgaa actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg
     3481 atgcatcggc ctcaacgtcg aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg
     3541 gcatgacaca gaatcgggct gagatggggg tttctgcgtt ccttgggaaa atgggcatta
     3601 gtatgaatga cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg
     3661 atcgcgtggc ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta
     3721 tggcttcgta tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct
     3781 gctgtcacgg aaaaaaattt attgaaagcg aggtttttac aaacaag
//
LOCUS       DAZPEP010000264         3798 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZPEP010000264 DAZPEP010000000
VERSION     DAZPEP010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.266"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..82
                     /locus_tag="ACHUT9_004253"
     CDS             <1..82
                     /locus_tag="ACHUT9_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350569.1"
                     /translation="REWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHUT9_004254"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="ACHUT9_004254"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFR1350570.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="ACHUT9_004255"
     CDS             1028..1486
                     /locus_tag="ACHUT9_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFR1350571.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="ACHUT9_004256"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="ACHUT9_004256"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFR1350572.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHUT9_004257"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="ACHUT9_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFR1350573.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="ACHUT9_004258"
     CDS             2692..3369
                     /locus_tag="ACHUT9_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFR1350574.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHUT9_004259"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="ACHUT9_004259"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFR1350575.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          903 c          990 g          954 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 ttcacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAZPEP010000265         3733 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZPEP010000265 DAZPEP010000000
VERSION     DAZPEP010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.267"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..181)
                     /locus_tag="ACHUT9_004260"
     CDS             complement(<1..181)
                     /locus_tag="ACHUT9_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HFR1350576.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            425..682
                     /locus_tag="ACHUT9_004261"
     CDS             425..682
                     /locus_tag="ACHUT9_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFR1350577.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHUT9_004262"
     CDS             complement(732..1682)
                     /gene="uspE"
                     /locus_tag="ACHUT9_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFR1350578.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHUT9_004263"
     CDS             complement(1834..2586)
                     /gene="fnr"
                     /locus_tag="ACHUT9_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFR1350579.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHUT9_004264"
     CDS             complement(2781..3296)
                     /gene="ogt"
                     /locus_tag="ACHUT9_004264"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFR1350580.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3307..>3733)
                     /locus_tag="ACHUT9_004265"
     CDS             complement(3307..>3733)
                     /locus_tag="ACHUT9_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HFR1350581.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1002 a          886 c          893 g          952 t
ORIGIN      
        1 taatgctacc aacagcactg ataattatgg tgtagattaa aaattgcacc gcatggaata
       61 aaaaaccttt tcttttacga tttccgcggc gaaaccaaaa gctcatcaga atcaatgctg
      121 cgcagctacc gaaaataatg accagattaa gcgcattatt tgtaaacagt tcagcgatca
      181 ttgttttatc aggctcctcc agataattgt cgtcatgccg gaaacccctg gcggggctat
      241 tttaccgcga caattcattc agatcatcaa tagtcaggga aggaagtagc aacattagct
      301 aactaatcaa tgcgttgatt gtaaatccag ctaagaggtg aggttttcag agcagacaac
      361 ggtgaaatgt catggtattg ttacgtttag gtaacaagaa atttgtctgc acaaggatta
      421 catcatgatt atggcgaaac tgaagtcagc gaaagggaaa aaatttctct ttggtttgtt
      481 ggcggttttc attattgcgg cgtcggttgt gactcgcgcg actatcggcg gcgttataga
      541 acagtacaat attccgctgt ctgagtggac gacatcaatg tatgtgattc agtcatcgat
      601 gatttttgtt tatagcctgg tctttactgt gttgctggca atcccgttgg gaatttattt
      661 ccttggcggc gaagagcagt aagtaaaaaa taggcccgat aactcgggcc ttgtcagtta
      721 ttgaagagtc gttaatcgtc ttcttcgtca tccagttcaa cgggtgtctg atactggtca
      781 ggtttaatga ccaacagatc gcagcgaaga tgatcaatca cctgttccgc cgtgttgccg
      841 aggaatgctg ctgaaatacc ggtgcgtcct accgtgccca gaaccacaat ccccgcctgt
      901 aagtgctccg ccaaatcagg aataacctct tccggcagac ctttttctac gtgcgtcatg
      961 ttttcattaa tgccgaattt ctgccgcaga gctttcattg ccagcaaatg ttgcccacga
     1021 atggcatcgt tataaacgct cggatcaaat tccggcagtt caatcgcgat attaattggc
     1081 gttactgggt aagcgccaac cagatgaact tcggtatggt tgacttgttc tgccagttcg
     1141 atcgtctctt tgaccagttt ttcattgagc gcattatgat acggctcttc actggcgaga
     1201 ttcaccgcca ccagcgcctt acctccttcc ggccacggct ggtctttcac catccacacc
     1261 gggcttgggc atttgcgtaa cagatgccag tccgttggcg taaaaatcac cgcttccaga
     1321 cggtcatgtt ggtgcgccat ttttagcacc aaatcgtgcc cgccgctgat cacttcctga
     1381 atgatggctt cgaagggacg gttatgccag accactttaa tttcaatggg aacgccagca
     1441 ttgagataat attttgcctg ctcgtggatc caggctgtac gctggctgat gacgccctga
     1501 cgcatagcgg tacgttcgtc cggggagagc agggtggtca tttcgtatga gaagtcatag
     1561 atcggcaaaa aggctttaat tttgccacca atccgttgat gtaaataaac agctcgccgc
     1621 aatgctggtt ggtcgtcctg gttaggatcg ataacaacga gcatgttctg atacatagcc
     1681 atacagggtc tccttacaac aactgtcaac gcagtttgta attaaaagat taacccatat
     1741 ctggtgaatg aaacagtgat gaaccttctg ccagatcaat aaatcagaaa aatttaatga
     1801 tatgacagaa ggatagtgag ttatgcggaa aaatcaggca acattacgcg tatgaccagc
     1861 aagctgggcc agcgcatcgt tattttcgat ggtgatgtat ttacctttga ctgccagcat
     1921 accgcttttc tggaagcgac ctagcagacg gctgatggtt tctaccgtca ggcccagata
     1981 gttaccgata tcgccacgag tcatcgtcag gcggaattca cgaggggaga agccgcgttg
     2041 ggcaaaacga cgggacaggt tgtagatgaa tgcagccaga cgttcctcgg catttttctt
     2101 cgacaacagc aagatcatgt cctgatcgcc tttgatttca ccgctcatca gacgcatcat
     2161 ctgctgacgc agattcggca ttttaccgga caaatcgtcc agcgtttcga acgggatttc
     2221 acataccatc gaggtttcca gcgcctgagc gaagctcggg tgatggccgc tgccgatggc
     2281 gtcaaatccc accaggtcgc cagctaaatg gaaaccagtg atttgctcgt cgccttgctc
     2341 agtgatggta taacttttaa tcgtaccgga gcggatggca taaagcgatt taagttcatc
     2401 accagcctta aacagcgtct ggcctttctg aataggcttc ttccgctcaa tgatattatc
     2461 aagctgatca agctcatgtt cgttgagtgt gaacgggatg caaagctggc tgatgctgca
     2521 atcctggcaa tggatagcac aaccgccaga ctgaatgcgc cgtataattc gcttttccgg
     2581 gatcataggt ctgctcaagc cgtaattgat atttgtcaat tttaacatct ttttagggag
     2641 caagtaagtc taagcaaacc ttaacagcag agaattccga tattagatgt aaatatatgt
     2701 ctatctattt gaaaaccctt aagttgttaa gggtaacttt acataaaagt gtgaacaagc
     2761 tggcacaaat tgtttaatgt ttacagcaaa agataacctt cgtggcgcaa taaccactct
     2821 tttcgctgaa ctccgcctgc atatccggtc atggtgccgt ttcggccaat aacccgatgg
     2881 caaggtacga cgatgctgat gggattcgat ccgtttgccg caccaacggc acgcgccgcg
     2941 ccagggcggc ccaattgttc agccagttgg ccgtaatgca ttacctgtcc gcaggggata
     3001 gtgcgtagag ttttccagac ttcgcgctga aatggcgtcc cccctgtggc agtgggaagc
     3061 gtatcaataa tgctaagatt accggcaaaa taatcacgaa gcttgtcgct taaaccgcct
     3121 gggttggtgg cagaaatgcg ctcatagcct tctttgcgat aatggatgtc cagcagctgc
     3181 accatgcgtt cgctgtactc ttcccattca accgcccgca ggcgaaattg ctcatcgcaa
     3241 atcacccaca gtggacccag tggcgtggca attttttctt caagtaatct cagcatccgt
     3301 tctctcttaa gacaaacgtg ggtaaatccc cggacctatc ggcagaccaa caagatacca
     3361 cgccagcaac atcagcagcc ataccaccaa aaagataagc ggatagggca agactaacga
     3421 atagtaagta cccagtttcg cgtctggttt gtagcgttgc aggaatccaa gaaacagtgg
     3481 aacaaaagga gataccggcg ctaaaggcaa tacggatgag tcggcaatac gaaagaggat
     3541 ttgcgcaaat gccgggtgaa agccaagtag cataaacatt ggtacgaaaa tgggggccag
     3601 aatcgaccag attgcggaac cgctggcaat aaacatgcat aagaaagagg aaagcaacgc
     3661 cagaccgaca aacgccggga tgccgctaag ccctgaactt tccagtatat cggtcagccc
     3721 cacggcgatg aat
//
LOCUS       DAZPEP010000266         3674 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZPEP010000266 DAZPEP010000000
VERSION     DAZPEP010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.268"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..95
                     /locus_tag="ACHUT9_004266"
     CDS             <1..95
                     /locus_tag="ACHUT9_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFR1350582.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="ACHUT9_004267"
     CDS             244..1491
                     /locus_tag="ACHUT9_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFR1350583.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHUT9_004268"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="ACHUT9_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HFR1350584.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="ACHUT9_004269"
     CDS             complement(2343..2687)
                     /locus_tag="ACHUT9_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350585.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..3531
                     /locus_tag="ACHUT9_004270"
     CDS             2929..3531
                     /locus_tag="ACHUT9_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350586.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
BASE COUNT          919 a          740 c          845 g         1170 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctaggat tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatggg gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac actaacttat
     3301 tttttgcctg gagagacatc gtcttatgtt gcaccgtatc ggtatacttc cggtagttca
     3361 aaaagttgtt ctggagctga ggtggatgac cccgatctac atgagaatat tcgcatttgc
     3421 tatccgtatg gcaattatga gtacgataat attatctatg ttgaaaagaa aattaataca
     3481 ttaggtgcgg tagtgacata tgcacagacc gcgcgtgatg atactgaatg agatagtata
     3541 tagcgggcaa gttttagtta atttatcgag gtaatataat ttacctcgac tcgtttattc
     3601 tggtatgaat atttcgcttt acgatcgatt tttatctgat gatatcatgc ggttttcata
     3661 tactgactta ctgt
//
LOCUS       DAZPEP010000267         3670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZPEP010000267 DAZPEP010000000
VERSION     DAZPEP010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.269"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..119)
                     /locus_tag="ACHUT9_004271"
     CDS             complement(<1..119)
                     /locus_tag="ACHUT9_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350587.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLA"
     gene            complement(127..522)
                     /locus_tag="ACHUT9_004272"
     CDS             complement(127..522)
                     /locus_tag="ACHUT9_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1350588.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            complement(519..1097)
                     /locus_tag="ACHUT9_004273"
     CDS             complement(519..1097)
                     /locus_tag="ACHUT9_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350589.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1109..1462)
                     /locus_tag="ACHUT9_004274"
     CDS             complement(1109..1462)
                     /locus_tag="ACHUT9_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1350590.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1474..1872)
                     /locus_tag="ACHUT9_004275"
     CDS             complement(1474..1872)
                     /locus_tag="ACHUT9_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1350591.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1914..2939)
                     /locus_tag="ACHUT9_004276"
     CDS             complement(1914..2939)
                     /locus_tag="ACHUT9_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1350592.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(2995..3327)
                     /locus_tag="ACHUT9_004277"
     CDS             complement(2995..3327)
                     /locus_tag="ACHUT9_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1350593.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            complement(3337..>3670)
                     /locus_tag="ACHUT9_004278"
     CDS             complement(3337..>3670)
                     /locus_tag="ACHUT9_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1350594.1"
                     /translation="VVQATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGR
                     EEQARVLAETPGMTVETARRILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAV
                     NDLLNTPV"
BASE COUNT          767 a         1124 c          959 g          820 t
ORIGIN      
        1 gccagacgcg accagtcaac gtctgaaagc gggttcgcat aagggtcacc gctcccctta
       61 taaacccaca gggtggtccc ggcacctttc accggcattg taggatttgg tacaggcata
      121 gcgtcctcac atttcataga taatgacata agtcagatcg gctgaactcc acaggcccgc
      181 atcatcgtcg cgccggtagt catagccgct ggccaccata ctggtgatca aatctgacag
      241 tgccgggata tcgctcatca ccggataaat ccgggactcc atccacgaat ccagctctga
      301 atccggcacc tgagcaggca ggaaaacttc aatatgcagc tccgcctgcc aggtatcgct
      361 gtccagctct tcgcccgtgt attcagcgcc ggtgagataa acggcaattg ccggaaaatc
      421 ttcctcatca aaaacagcgg ggcgaccatc aaaaagcgtc gccccggtgt catgcttctc
      481 cagtgcatcc agtacggctg cacggagttc agtatgtttc atcgctttat taccattctc
      541 agttgatgct gcagcgcata gcccagctct ttcggaagac gttcacgccg tatccgttca
      601 atattctgtt taaacgccgt ggtcagcggc accgccatcg ggattttcac cacatcaatg
      661 gggtaacggt ttttcccggc cacacgctgc ataacatgcc accggccgtt tttcagttgc
      721 tgaataaacg cgccgggaat acgacggtta cccaccacaa gcacgctgcc gccacctttc
      781 agggatgaac gctgcccctt tttatgacgc ctgcggcggg acaggacaac ccgcgcatta
      841 cccagcttga ttacgggcaa atccccccgg ttaactttga ttctggcctg cggatttttg
      901 accgtggccc ttttcagcct ggccctttcc tttaccagtt tccggcgtac ctttgtctca
      961 cgggcaacct gtgacgcaga ctgcgatatc gcggatgaag caacgcggtt aatggccatt
     1021 gcggcggcac cgggcaccgc cgttttgctg atacggctga ggttttcaac ggcctgctca
     1081 agacctttta tggccataca tccccctttc agcggcgacg gttaacggca ggcggtacgc
     1141 cccgtccaag ccagagatga caacttccgc catcatccgg cgaaacccga tctacccaga
     1201 aattttcctc accgatggtc agcgtgtctc cacgccgcag ctgccgcacc tcatcagtcc
     1261 ggacaaacag ggacgggctg gagccttcaa cgcgcacgcc ctgtccggca tagctgatat
     1321 tttcagggtc atcaaaaaca ccacgtatca ccgcacctga ctgctcaccg gatgtcatgg
     1381 tggctgacgt tcccatgtac ccgcgtatcg tttcatcggc gcgggcaatg gcagcatcga
     1441 acaggttatc gaaatcagcc acagcgcctc ccgttattgc attctggcca ggccgcgctc
     1501 tgtcatttcg gctgccacac cggcagagac acgaaacgcc gttcccggca gcacaaatgc
     1561 cacaggttta tcccgcgtgg cgtgaagtgc atcagtatgc agcgtcacca gtgccacgac
     1621 cgtgaccagt tcagccgtat cctgaatcac ggtatccggc tgcgctgata ccacctcatt
     1681 ttcatgcccg gtcagcacat tttcccggct gagaggggtg tcctgaccgg cagtttcatc
     1741 cgtgtcatca agctcttctt ccagctctgc cacacgaagc gccagttctt ctttcgttcc
     1801 cgtcaaactg acatcgcggt tcaattgctc acccagctcc tgaagacggg caatcagttc
     1861 atctttcgtc atgaactcct ccacagagag aaaatggccc cgaagggcca tgattacgcc
     1921 agttgaacgg acacgaactc atcaggatca gccagcagca tcagcggtgc tgactgaatc
     1981 atggtgaact cacgcgccgg atcgccggtg gtcacccagt ttttcgggta acgggcagag
     2041 gcgttaatgc cttcgcgctg tgcgtccgca tcctgaatgc agccataggt gcgcagaccg
     2101 cgagcctgag tgttccccag caccatcgtg ttgtccggca ggaagttctt tttgacgccg
     2161 ttttccacgt actgtccgga atacacgacg atggccacat cgccatacat tcccttatag
     2221 gacaccgctt tgcccaggtc ttttaccgct gtctccagct cggaatgaga gccgcgacgg
     2281 gtatccagct tctccttgac ggctttgaag gaacggaaca gcgcccagcc tttcgggtca
     2341 aacacgatga tattcaccac accgctggcg ttcagcgcgt aggcttcgat atcgtcggtc
     2401 gggtcatacg tggacttgtc acgcttgctc cactccgtga cgccggactg tgtgatgttg
     2461 ttcgccgcac tgcggcccat atccacctca accggatcga aggcttcacc ggtcatggtg
     2521 tatttgccct tgagcacggc agaaactgcc tgcatctctt cgacctgagc aatggccagc
     2581 tcttcgtcac gcatgttctg catgatgatg cgacggcggc ggtaagccgg gtccgccaga
     2641 ttctgcggat cttcatccgg caggcgacgc agggtcatct gcggattcac ctcatgcttg
     2701 ggtttgacat atcccggcgt aaattcagag gtggagccgc cacgggaacg gataacctca
     2761 ccggaaacaa tcggcgaaac gtacagcgcc atgtttacca gtcccggaat ttgtgagaga
     2821 tagactttct ccgtagtgaa gggatagctc tcacggaaaa agagacgcag aaacagcgga
     2881 tcaaacttaa atttctgctc atttgccgcc agcagctggg cggttgtgta catcgacata
     2941 aaaaaatccc gtaaaaaaag ccgcacaggc ggcctttagt gatgaagggt aaagttaaac
     3001 gatgctgatt gccgttccgg caaacacggt ccgttttttc gtctcgtcgc tggcagcctc
     3061 cggccagagc acatcctcat aacggaacgt gccggacttg tagaacgtca gtgtggtgct
     3121 ggtctggtca gcagcaaccg caagaatgcc aacggcagca ccgtcggtgg tgccatccca
     3181 cgcaaccagc ttacgggtgg aggtgtccag catcagcggg gtcattgcag gcgctttcgc
     3241 actcaatccg ccgggcgcgg ttgcggtatg agccgggtca ctattgccca gcggctggta
     3301 atgggtaaag gtttctttgc tcgtcataaa catcccttac actggtgtgt tcagcaaatc
     3361 gttaacggca tcagatgccg ggttacctgc agccagcggt gccggtgccc cctgcatcag
     3421 acgatccagc gcagtgtcac tgcgcgtctg tgcactctgt ggtgctgcgg ccagaatacg
     3481 gcgggccgtt tccacggtca taccgggggt ttcggccagc acgcgtgcct gttcttcgcg
     3541 tccgtgagcc tcctcacagt tgaggatccc cataatgcga ctgttttctg ccgcaaccgc
     3601 tgcggtgatc tgcgcgttca cgtccggctg cgccgcgctg gcgttctcgc cctccgtcgc
     3661 ttgcaccacg
//
LOCUS       DAZPEP010000268         3667 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZPEP010000268 DAZPEP010000000
VERSION     DAZPEP010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.270"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..119)
                     /locus_tag="ACHUT9_004279"
     CDS             complement(<1..119)
                     /locus_tag="ACHUT9_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350595.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYKGNGDPYANPLSDVDWSRLA"
     gene            complement(127..522)
                     /locus_tag="ACHUT9_004280"
     CDS             complement(127..522)
                     /locus_tag="ACHUT9_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFR1350596.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            complement(519..1097)
                     /locus_tag="ACHUT9_004281"
     CDS             complement(519..1097)
                     /locus_tag="ACHUT9_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350597.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSAAQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIEQIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(1109..1462)
                     /locus_tag="ACHUT9_004282"
     CDS             complement(1109..1462)
                     /locus_tag="ACHUT9_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFR1350598.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1474..1869)
                     /locus_tag="ACHUT9_004283"
     CDS             complement(1474..1869)
                     /locus_tag="ACHUT9_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFR1350599.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDTAGQDTPLSPENVLTGHENEVVSAQPDTVTDTAELVTVVALVTLHTDALHAIRDEP
                     VAFVLPGTAFRVSPGVAAEMTERGLARMQ"
     gene            complement(1911..2936)
                     /locus_tag="ACHUT9_004284"
     CDS             complement(1911..2936)
                     /locus_tag="ACHUT9_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFR1350600.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVIDIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(2992..3324)
                     /locus_tag="ACHUT9_004285"
     CDS             complement(2992..3324)
                     /locus_tag="ACHUT9_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFR1350601.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKTPAMTPLMPDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(3334..>3667)
                     /locus_tag="ACHUT9_004286"
     CDS             complement(3334..>3667)
                     /locus_tag="ACHUT9_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1350602.1"
                     /translation="VVPATEGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGR
                     EEQARVLAETPGMTVETARRILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAV
                     NDLLNTPV"
BASE COUNT          761 a         1127 c          967 g          812 t
ORIGIN      
        1 gccagacgcg accagtcaac gtctgaaagc gggttcgcat aagggtcacc gttcccctta
       61 taaacccaca gggtggttcc ggcccctttc accggtgcca gaggatttgg tgttggcata
      121 tcgtcctcac atttcatagg taatgacata agtcagatcg gctgaactcc acaggcccgc
      181 atcatcgtcg cgccggtagt catagccact ggccaccata ctggtgatca aatctgacag
      241 tgccgggata tcgctcatca ccggataaat ccgggactcc atccacgcat ccagctctga
      301 atccggcacc tgagcaggca ggaaaacttc aatatgcagc tccgcctgcc aggtatcgct
      361 gtccagctct tcgcccgtgt attcagcgcc ggtgagataa acggcaactg ccggaaaatc
      421 cgcctcatca aaaacagcgg ggcgaccatc aaaaagcgtc gccccggtgt catgcttctc
      481 cagtgcatcc agtacggctg cacggagttc agtatgtttc atcgctttat taccatccta
      541 agttgatgct gcagcgcata gcccagctct ttcggaagac gttcacgccg tatctgctca
      601 atattctgtt taaacgccgt ggtcagcggc accgccatcg ggattttcac cacatcaatg
      661 gggtaacggt ttttcccggc cacacgctgc atgacatgcc accggccatt tttcagttgc
      721 tgaataaacg cgccgggaat acgacggttt cccaccacaa gcacgctgcc gccacctttc
      781 agggatgaac gctgcccctt tttacgacgc ctgcggcggg acaggacaac ccgcgcattc
      841 cccagcttga ttacgggcaa atccccccgg ttaaccttga ttctggcctg cggatttttg
      901 accgtggccc ttttcagcct ggccctttcc tttaccagtt tccggcgtac ctttgtctca
      961 cgggcaacct gtgccgccga ctgcgatatc gcggatgaag caacgcggtt aatggccatt
     1021 gctgacgcac caggcaccgc cgttttgctg atacggctga ggttttcaac ggcctgctca
     1081 agacctttta tggccataca tccccctttc agcggcgacg gttaacggca ggcggtacgc
     1141 cccgcccaag ccagagatgg cagcttccgc catcatccgg cgaaacccgg tctacccaga
     1201 agttttcctc accgatggtc agcgtgtctc cacgccgcag ctgccgcaca tcatcagtcc
     1261 ggacaaacag ggacgggctg gagccttcaa cgcgcacgcc ctgtccggca tagctgatat
     1321 tttcagggtc atcaaaaaca ccacgtatca cagcacctga ctgctcaccg gatgtcatgg
     1381 tggctgacgt tcccatgtac ccgcgtatcg tttcatcggc gcgggcaatg gcagcatcga
     1441 acaggttatc gaaatcagcc acagcgcctc ccgtcattgc attctggcca ggccgcgctc
     1501 tgtcatttcg gcggccacac cgggagagac acgaaacgcc gttcccggca gcacaaatgc
     1561 cacaggttca tcccggatgg cgtgaagtgc atcggtgtgc agagtcacca gtgccacgac
     1621 cgtgaccagt tcagccgtat cagtcacggt atccggctgc gctgatacca cctcattttc
     1681 atgcccggtc agcacatttt ccgggctgag aggggtgtcc tgaccggcag tgtcatccgt
     1741 gtcatcaagc tcctcttcca gctctgccac acggagcgcc agttcttctt tcgtccccgt
     1801 caggctgaca tcacggttca gttgctcacc caacgaccgg agacgggcaa tcagttcatc
     1861 tttcgtcatg gactcctcca cagagagaaa atggccccga agggccatga ttacgccagt
     1921 tgtacggaca cgaactcatc agggtcagcc agcagcatca gcggtgctga ctgaatcatg
     1981 gtgaactctc gcgccggatc gccggatgtc ttccagtttt tcggataacg gggagacgca
     2041 ttaataccct cactcaatgc atccgcatcc tgaatacagc cataggtgcg cagaccgcgt
     2101 gcatgagtgt tacccagcac catcgtgttg tccggcagga agttcttttt gacgccgttt
     2161 tccacgtact gtccggaata cacgacgatg gccacatcgc catacattcc cttataagac
     2221 accgctttgc ccaggtcttt taccgctgtc tccagctcgg aatgagagcc acgacgggta
     2281 tccagcttct ccctgacggc tttgaaggaa cggaacagcg cccagccttt cggatcaaac
     2341 acgatgatat caatcacacc gctggcgttc agcgcatagg cttcgatatc gtcggtcggg
     2401 tcatacgtgg acttgtcacg cttgctccac tccgtgccac cggactgtgt gatgttgttc
     2461 gcggcactgc ggcccatatc cacctcaacc ggatcgaagg cttcaccggt catggtgtat
     2521 ttgcccttaa gcacggcaga aactgcctgc atctcttcga cctgagcaat ggccagctct
     2581 tcgtcacgca tgttctgcat gatgatgcga cggcggcggt aagccgggtc cgccagattc
     2641 tgcggatctt catccggcag gcgacgcagg gtcatctgcg gattcacctc atgcttcggc
     2701 ttgacatatc ccggcgtaaa ttcggaggtg gagccgccac gggagcggat aacctcaccg
     2761 gaaacaatcg gcgaaacgta cagcgccatg tttaccagtc ccggaatttg tgagagatag
     2821 actttctccg tggtgaaggg atagctttca cggaaaaaga gacgcagaaa cagcggatca
     2881 aacttaaatt tctgctcatt tgccgccagc agctgggcgg ttgtgtacat cgacataaaa
     2941 aaatcccgta aaaaaagccg cacaggcggc ctttagtgat gatgggtaaa gttaaacgat
     3001 gctgattgcc gttccggcaa acgcggtccg ttttttcgtc tcgtcgctgg cagcctccgg
     3061 ccagagcaca tcctcataac ggaacgtgcc ggacttgtag aacgtcagcg tggtgctggt
     3121 ctggtcagca gcaaccgcaa gaatgccaac ggcagcaccg tcggtggtgc catcccacgc
     3181 aaccagctta cgggtggagg tgtccggcat cagcggggtc attgcaggcg ttttcgcact
     3241 caatccgccg ggcgcagttg cggtatgagc cgggtcactg ttgcccagcg gctggtaatg
     3301 ggtaaaggtt tctttgctcg tcataaacat cccttacact ggtgtgttca gcaaatcgtt
     3361 aacggcatca gatgccgggt tacctgcagc cagcggtgcc ggtgccccct gcatcagacg
     3421 atccagcgca gtgtcactgc gcgcctgtgc actctgtggt gctgcggcca gaatgcggcg
     3481 ggccgtttcc acggtcatac cgggggtttc agccagcacg cgggcctgtt cttcgcgtcc
     3541 gtgagcctcc tcacagttga ggatccccat aatgcggctg ttttctgccg caaccgctgc
     3601 ggtgatctgc gcgttcacgt ccggttgcgc cgcgctggcg ttttcgccct ccgtcgctgg
     3661 caccacg
//
LOCUS       DAZPEP010000269         3632 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZPEP010000269 DAZPEP010000000
VERSION     DAZPEP010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.271"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(94..657)
                     /locus_tag="ACHUT9_004287"
     CDS             complement(94..657)
                     /locus_tag="ACHUT9_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFR1350603.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(761..979)
                     /locus_tag="ACHUT9_004288"
     CDS             complement(761..979)
                     /locus_tag="ACHUT9_004288"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350604.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1138..1524)
                     /locus_tag="ACHUT9_004289"
     CDS             complement(1138..1524)
                     /locus_tag="ACHUT9_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFR1350605.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1514..1885)
                     /locus_tag="ACHUT9_004290"
     CDS             complement(1514..1885)
                     /locus_tag="ACHUT9_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1350606.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1885..2142)
                     /locus_tag="ACHUT9_004291"
     CDS             complement(1885..2142)
                     /locus_tag="ACHUT9_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350607.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2139..3530)
                     /locus_tag="ACHUT9_004292"
     CDS             complement(2139..3530)
                     /locus_tag="ACHUT9_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFR1350608.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3527..>3632)
                     /locus_tag="ACHUT9_004293"
     CDS             complement(3527..>3632)
                     /locus_tag="ACHUT9_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1350609.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1016 c          812 g          954 t
ORIGIN      
        1 ccgctcatcg gcgggtttaa gctgtgtggc gtagtaacca ctcttaacag gatattcaac
       61 tttttacgat cgtaaagttt tcggaaaaaa tttttaaaac cgcatcaacc gctccaccag
      121 ttgctcttta cgggcaacga tccacccgtg ttgctccaga taaaatttaa accgttccag
      181 agtacatacc atcgcatcgg cgggtacctt ttccgtgaac tcaacctgac cgtgtttatc
      241 gaagtggctc agtaatgcgc atccatcatt ttcgatagat gtgttttgtg ccgctggtgg
      301 ctgtttctgg ttgaaataac agtcttccag tttttcgaac acttcccacg cctgatcggt
      361 ttcgagcatt ttggcgtgac gggctgcgcc acgttctgtc cagaggatga gggagcgaac
      421 gttacgggca attttcacag aaactcttaa agagttttcg ttcttcaact ctcgtaacgc
      481 atcaccttct atcaggaaaa aatgtttacc ttctataaac cgttctttgt ttcgattgaa
      541 attgtttgtc aaacgctggc gctctgttcc gtacaaatca gccagcagct ctgttgtcat
      601 gacaggaaga tggttatgcg taagtgacgg gagtttttca atagacgtgc gattcataga
      661 catgtccttt cgaatatttt gatttacccc ttttgagagg gtgaccgggc gctcaaaacc
      721 gtcgaaagtc ggcgggcgta tttccattgc tggtattgta ttagccgcac acccggtcat
      781 aaaccaagaa ttctggacac aaaaaaacca catggctatc gggtgtggat accgctttcg
      841 aggtgttttg agcaccgcgg ggaaatgtaa actgcaaacc tgaaactgtc aacaccgtca
      901 ggttccaatc atcgggtggt gagacgcaca gggttggaac taccgggaaa ccgaccggcg
      961 agcttttcag ctcccccatg cgccccacca taattcagat gtgcgcgtgc atacgacaat
     1021 aaaaaacacg ctcgcggcgt gtgtctgtcg cggtctctat ccggggttcc aatcccgacg
     1081 ccagattttg ctggcgcgtg aggaatatag ccccggacaa tgtgtctggt caagcaccta
     1141 cataattcgt tctacgtatc tgtccatctc cagccggata tcaagcatca tcaacatgcc
     1201 atcaataacc ccttccgctt tctgcaggcg cttgccaata caggtatccg aacacccatg
     1261 tttccgtgcc agactcataa aagtcattcc acctacgtaa taatccacca gcaaatcgtg
     1321 caaatcctga tttttcttgt tcaaccgggc catacagcca caaattatca ttgcatcatc
     1381 atcagaacac tgagggcgtg atttcacttt cgacggaatt aaccctttaa aaccagcagc
     1441 gatcgacgcc caggatacat cttcatgagt gtttgcagcc caagcccccc accgttccat
     1501 aacctgctga atatcacgcg ccatcgttat cacctgtgat ttcataaatc ttcacgccca
     1561 gtcgcccacc aggaacgagc tgaccgcgca caatattgat ttcatcaaac tgctcgtcgt
     1621 ctatgagtag tccggcatgc gtcagcgcat ccagtggtgc tttcaggata ttgtccaggt
     1681 cacgacggcg cttatccggt ggctctgcaa taatctttat cgccagcctt ccggacaggt
     1741 ttaatttcag ccgctgctgg cgaacaatta gcgtcacatc acggcgataa cgctcaccaa
     1801 cttttgatac aaaatatgtg ctgccacgac gtcgccagta agtattcacc gtcggcggat
     1861 aaggtaaaat aaactcatgg cgcatcagcg cagcacctcc tgcaccattt tttcaaactt
     1921 tccaactttg gtttccagct ctgccacaca atcaacgagc tcatccaccg ctttttgtgc
     1981 gcgatgtttc gcctgcatca gttccctgag cgctggcacc atatcccgac gaatggcatc
     2041 ttttgttaca cctgtttttt ccagttgttc cgcctgtcgc aacatttcct gcgcgtgttt
     2101 acgcaattgt tcagaggtaa aagtcattgt ctggttgttc aaaagaaacg ctccatctta
     2161 ctgctgtcgg tacgtgtatt gctgtatctg cgcggctggg gctgccgcat tggggtggaa
     2221 agaacctgtg cgctttcctg gtccacaggc agaaaatgtc cgttataaaa acgccggtaa
     2281 atcgtcccca gagaaccgtt acgttgtttc gtgatattaa tttctgctat gcctgtagcc
     2341 tgtgtatccg ggttgtacac ttcatccctg taaagcatca gaatgatgtc tgcatccgcc
     2401 tctatttctc cggaattttt caggtctgag ttcatgggac gtttattggg tctggactcc
     2461 acaccgcggg agagctggct cagcgcaatc aacggaaaac caccggattt tgccaggcct
     2521 ttaagcccct ttgagatttc acccacggca aggtcatgac gccccgtggt tcgggttttt
     2581 atcagcccga gataatcaac caccaccagc gccgtttccg gatgtttaat cagatggtgt
     2641 ttcgttgttg cgcatatctc atcaatggtc aggttcgcct ggtccaccat ccagatattg
     2701 cgcccggtca tccgccccac cccttgtgag aaacgcgccc agtcttcatc ttcaaagtga
     2761 gccacagatt tcaggcgtga tactggcatc cctccagccg cagacaccat acgttcacca
     2821 atctggatgt tcgccatctc catggtgaac agaagcacac catgcccctg ctcagtcacc
     2881 ttgtcgatga tgtccagcgc aagttcggtt ttccccatcg aaggacgggc ggcaatgaat
     2941 accaggtctc cgggctccat accgcctgtt tttgcgtcca gttcatcaat accggtcatc
     3001 aacgtcctgg atttctccag cccctgattc cggcattcaa cacgctcaac cacttccgga
     3061 agcacatcat caatatgtac cggctgaatg acgccctttc ctgtcgacag tgtgaccatc
     3121 atgttctgcg catccttcag ggcatcttca gctgcttcac aggtatgcgc atcacgtaat
     3181 ttctgcagcg cctcattcag tgttttttct gcatcgcgca gtgcggcatt gcgccgcaac
     3241 gctgcaacat agtgctccag tgaagacttc acccaggttt tacgcccggt atcagtaatc
     3301 accggggcaa gttccggcat ctcattacac aacagcacgg ggtcaatcac tcctgaaaca
     3361 cgggcctgtc tgcagatgcc tgtgtagata tcccgatacg ctcgtacaga aaaaatgtcc
     3421 gccggtagtg tgatcagaat atccatcact tcatgatctg ccccacgcag aaagaatgcg
     3481 ccaatgacag cgccttccag gtcatcatta cgccagactg gtgttgtcat gcagccacac
     3541 ctctgataca agaacggtag ctgggccagt tgaacgacaa ccagttgcgc cccccgtctg
     3601 tgatcctgtc ggcaatgcgg gggctgatga ac
//
LOCUS       DAZPEP010000270         3628 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZPEP010000270 DAZPEP010000000
VERSION     DAZPEP010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3628
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.272"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            948..1634
                     /locus_tag="ACHUT9_004294"
                     /pseudo
     CDS             948..1634
                     /locus_tag="ACHUT9_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1967..2842
                     /locus_tag="ACHUT9_004295"
     CDS             1967..2842
                     /locus_tag="ACHUT9_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFR1350610.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2850..3626
                     /locus_tag="ACHUT9_004296"
     CDS             2850..3626
                     /locus_tag="ACHUT9_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFR1350611.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT         1010 a          826 c          842 g          950 t
ORIGIN      
        1 tatattaagg tctgttttct tcttagaaaa ataattcttt gttctaatta ttatagatgt
       61 aatcccccaa acaataaaaa gaatggagct gcttgtaatt atttgatata tcaaaattgt
      121 tttcctctat ttttgtgtag atagatgtta atggcactgg gtgcaatcaa ttaagaaaat
      181 tgacgccaga ctaattatta ataatccagt tatcttttta ttcatatcat ctcctttcca
      241 ttggttaatt tatcggaaat tcatccccac taactaaaac gatccacaca ataaaactaa
      301 atctaattgc aggcaatcaa ttccagtcga tatatcatct gtatggaaat catcgctcat
      361 cactcatcgc agcctgaaag cccagagcaa cggggcaggt ctgcatataa gtggttacac
      421 ggcagaattc cagagggttc tgtcactaaa ttcagtcact gacaaatttg catgatgttg
      481 aatagacgag attttgtgtc gaaatagttg tttcagtaac tgtcactcaa tcggtggttt
      541 atgtgactat attcgcaaaa actggtcgca gaaaagtcct gttttcagct tggaaacccg
      601 gtaatcagaa taatggatag gggaacaaaa atctctgtat agaacgctcc attgagggag
      661 agaaaacgtg tcattttgac ccgttcggta ttcagaacac acaaaaccga tcgcgggtcg
      721 ctttcgtgca tagttatgca acgcgctaaa aacgattctg acgcattttt tatgtttacc
      781 ctgttttttc ttgtcttttt gagttttttg cgtcagagag cgtctgagag ggcacgtaca
      841 acgggagtta tggtaaatgg atcgggtttt cgggaaggtt cgacaggatt tgccgttggg
      901 tgtagtgtaa gcgactgaaa aacaaacgcc cctgaaatca tgtccagatc cggcagaacc
      961 agcctcatgc gcagttctgg ctggtttttg acgttgatcg cgagggggca gcgattgact
     1021 ggagcgaccg gaacgctccc gcgccgaaca tcaccgtaaa aaatcctgtg aacggtcatg
     1081 ctcacctgct ctacgcgctc aatatcgccg tgagaaccgc gccggacagc tcggttaagg
     1141 cgctgaaata cgccgctgcg attgaacgtt cgctgtgtga gaaactttgt gctgatgtga
     1201 actacagcgg actgatttgt aaaaatccgt tccacctgga atggcaggtg atggagtggc
     1261 gagaggaagc ctataccctc gatgaactgg ctgattatct cgatttgagc gcctcagcgc
     1321 gccgtagcat cgataaacat tacgggatgg ggcgaaactg ccacctgttc gaaatgacgc
     1381 gcaaatgggc ttacagggcg attcgtcagg gctggcccga attctcacag tggcttgatg
     1441 ccgtgattca gcgtgtcgaa atgtacaacg catcgcttcc cgttccgctt tcaccgcctg
     1501 aatgtcgggc tattggcaag agcattgcga aatacacgca caggaacttc acgccggaaa
     1561 ctttcgcaca gtatgtggct gatacgcaca cattaacgta cgttttcgtt ccattggccc
     1621 tcaccccccg ataacgaccc aggtagcgcg ccgtattccc cccgccggcc gttttcttcg
     1681 acatgtacat caggcgcgga actcatcggg gcgcgggaat gaaatcccat tcagactccg
     1741 gatagattca agcaagccca cttatcgtca aaatcggtgt tgcaaaaacg ggagtgacca
     1801 tagatccgtt ttccaagcgg accccataat atcggctggt aaacatcact cgctagtgca
     1861 ttctttttgt tggtaatgat aatcatttac attcattctt tgatgtttta tcataattaa
     1921 tgttttgtca ataaatatat ggaaacatat tgtttcagat gggaatatga gaaagttcat
     1981 ccttatttct atgataacgt tagctagtgt tagtctggga gcctgcagac agaatgtcac
     2041 cataaagcag gatgcgccag ggcagaaagc tttcctgact gacgggcaga tttacgattt
     2101 gcacagtggc aaaattattt ctagcagcga gcttttggcg gatcttgcta ccgctcgaca
     2161 tcttattatt ggtgaaaaac acgataatgc cgagcatcat cagattgagc tttggcttat
     2221 tcaaaatttg ctcatccagc gaccacaggg gagtgtgttg cttgagatgt tgacgtcaga
     2281 gcaacagccc cgagttaacc aagttaaatg ttggctgaaa gataatcctg tggttcgaga
     2341 tagccgtgtt caggaactac ttaactggca aaaagggtgg tcctgggaga tgtatggcga
     2401 catcgtcatg cagctattac gtgggcctta cccgctgctc aatgccaaca ttggccggga
     2461 gcagatgttg gcactgtaca aaaaaaacga atttcctaaa ggaaaaaaat ccactgcacc
     2521 tgtggtacaa gaagctctgc gtgaaaccat catatctatg catgaaggca accttgaaag
     2581 ccagcaactg acgtcaatgc tctctattca gcagcagcgg gatcgctaca tggcccgcca
     2641 gcttctgagt gccccggtac cgtcgttgtt gattgcaggt ggttatcacg cgtcaaaaag
     2701 catgggcgta ccgttacaca tggaagatct tgccaccggc acccatccag tggtgttgat
     2761 gcttgcagaa aaaggaatga acatcacagt agatcatgct gactacgtgt ggtttgtagc
     2821 gccagataca acaaaaaggt aaaccatgga tgatgaatat tctccacttc tcacagtcgg
     2881 taaaatggag cagctggttt atctgttctc tactgcttca tggactgatt ttccttgcgt
     2941 ttatctggcg tttttccgaa gttcagcccg ctatgtctcc tgcaccggcg atcatgctgc
     3001 aatgggcaga aattatagag gctccttctt ctccactttc gcttccggtg ggaatcgctc
     3061 aacaggagtc cgcagtgaca gaagaaaagc aacaaactga ggacagacaa caacgtcctg
     3121 tcactgaaga cagtgatgca accattgaaa taactaggaa gaaaaagtca tccgatggag
     3181 aaaagaaaaa gacacgtcca cctcgtaaga taaaggctca aaccagcgac agcaacccca
     3241 cagccgtttc cagtaatgct gcacctcagg ctctggtcga atcgtcccgt attgctgcgc
     3301 cttttaacag tgactcaaca aagcgagata attctgaggc atcttgggaa agcagagtaa
     3361 aaggccacct aaaccgttat aaacgttacc ctggcgatgc cagaaaacgt gcacgaaccg
     3421 gcacagccgt cgtgactttt accgtaaaca cggaaggcac tattgtcagt agtttcttgg
     3481 aaatctcttc aggaaccctt tctcttgacc gcgaagctat tgctgtttta gaacgcgctc
     3541 aaccacttcc caaaccaccg cctgaaatat tggagggagg attatttaaa gtaaaaatgc
     3601 cgattacctt taagttgaag gagtaaag
//
LOCUS       DAZPEP010000271         3624 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZPEP010000271 DAZPEP010000000
VERSION     DAZPEP010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.273"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1363)
                     /locus_tag="ACHUT9_004297"
     CDS             complement(<1..1363)
                     /locus_tag="ACHUT9_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFR1350612.1"
                     /translation="MNRTSPHYCRRSVLFLLISALIYAPPVMAAFTTNVIGVVNDETV
                     DGNQRVDERGTTNNTHIINHGQQSVYGGVSNGSLIESGGYQDVGRNNNYMGQSNNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNSHISGGTANGTIIDGGGQ
                     TVTTQGHVDGTTINKSGYQDITQGSMATNTIINGGRQYVEQSTVGTTTIKNGGEQRVY
                     ESHALDTTIEGGTQSLNNKSTAKNTQIYSGGTQIVDYTSSSDVIEVYSGGVLDVSGGT
                     ATNVTQHDGAILKTNTNGTTVSGTNSEGAFSIHNHVADNVLLENGGHLDINAYGSANK
                     TIIKDKGTMSVLTNAKADATRIDNGGVMDVTRNATNTIINGGTQNINNHGIATGTNIN
                     SGTQNIKSGGKADTTNISTGSRQVVEKDGTATGSNISAGGSLIVYTGGIAHGVNQETG
                     SALV"
     gene            complement(1428..1547)
                     /locus_tag="ACHUT9_004298"
     CDS             complement(1428..1547)
                     /locus_tag="ACHUT9_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFR1350613.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1771..1929)
                     /locus_tag="ACHUT9_004299"
                     /pseudo
     CDS             complement(1771..1929)
                     /locus_tag="ACHUT9_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2274..>3624)
                     /locus_tag="ACHUT9_004300"
     CDS             complement(2274..>3624)
                     /locus_tag="ACHUT9_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
                     /protein_id="HFR1350614.1"
                     /translation="AASGTYRGALETLQKMMSECIYHEGNAFVIMGSGEQLKRIKYDV
                     DENNLKVFNVYFDNNEELVTDGEPDVVCLSKQVWEDLLIKLKLENKENAVSETDLSSN
                     KNNVDQFIECAKRNEQTLFDNIRKSDFHVDSFKPGSTRSVILETQPNVCMEPHNLYDN
                     QIDKVSPVTKNSQQVKGHYGDKFKEMQMFLNQMSNALQQTPSLSESKEHTIDIQKITD
                     AFVKEFRGVLIDKNGNCSERLFNFYECCYKFLPRAQLQDKIESYNSALQAFSIFRSST
                     LNNNDVGFNFKLFPEVKLSGENLETVFKYKKGSFVREIARINITLQKEEDGLYNLGGL
                     DFKGCFFSGQNFSNYDIQYVNWGTSLFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSG
                     VLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         1006 a          820 c          644 g         1154 t
ORIGIN      
        1 caactaaagc actgcccgtc tcctggttaa ccccatgtgc tataccgccg gtatagacaa
       61 tcagcgagcc tccggcgcta atattgctgc cagttgccgt accatctttc tcaacaacct
      121 gccggctccc ggtggaaata tttgtcgtgt cagctttccc gccgctcttg atattttgtg
      181 ttccgctgtt gatattggta cccgtggcaa taccatgatt attaatattc tgtgtaccac
      241 cattgattat ggtattggtc gcgtttctgg taacatccat taccccgccg ttatctattc
      301 gggtcgcatc agctttagca ttcgttaaaa ctgacattgt tcctttatct ttaataatcg
      361 tcttatttgc cgaaccatat gcgtttatgt ctaaatgacc accgttttcc agcaacacat
      421 tgtctgccac gtgattgtgg atggagaatg caccttcact attcgtaccg ctcaccgtcg
      481 taccgttagt gtttgttttt aaaattgcac catcgtgctg ggtaacattt gttgccgtac
      541 caccactaac atcaagcacg ccaccggaat aaacttcaat aacatccgag gagctggtgt
      601 aatcaacaat ttgcgtacca ccagaataga tctgagtatt ttttgccgtt gacttattat
      661 tcagagactg agttccacct tcaatcgtcg tgtccagcgc atggctttca tatactcttt
      721 gctcaccgcc atttttaatg gtggttgttc ctactgtgct ctgttcaaca tactgtcgtc
      781 caccatttat gattgtgttc gttgccatac ttccttgcgt gatgtcctga taaccggact
      841 tatttatcgt tgtaccatca acatgcccct gagttgttac tgtctgccct ccaccatcaa
      901 tgatggttcc attcgcagtc cccccactta tgtgtgaatt accaccctta attgtcgttc
      961 cattgctgat accacctgta taaacgtcct gattgccact gtcgattatc gtaccggtgg
     1021 aaatgccccc gtcatgaatt gtctgtcttc ctccgttaat ggttgtatta ttagactgcc
     1081 ccatataatt gttattcctt ccaacatctt gatatccacc agattcaata agacttccat
     1141 tagatactcc gccataaaca ctctgctggc catggttgat aatatgagtg ttatttgttg
     1201 tacctcgttc atctactcgt tggttgccat ctacagtctc atcgtttacc acaccaataa
     1261 cattagtagt aaaggccgcc atcacgggcg gggcatatat caaggcagat atcaataaga
     1321 aaagtactga gcggcgacaa taatggggac tggtcctgtt cataaatttc atcctctgaa
     1381 aagtgaatac tgagtagcgt ttaagcgact ttagctctgc tgcaacatca gcccgcaggc
     1441 accagaccgg ggggttcatc ctgaagagac agcgcaagtg tattgtgttc accgctcatc
     1501 aaagacatca tgatgaaatg atgatgttcc acataagaaa gaggcatttt ttaaacacag
     1561 tgcgctgaag tgtgattgga taaaaaagtc aatctattca ggaaatacgg gcgtattctt
     1621 ttctttcgac aatcaggccg tcggcaaaat aaaatgattt acataatcgt ttctgatgaa
     1681 tatcttctgc tcacataaaa atcacacaat aactttgaga tcgcagattc ttttactttt
     1741 acagcattcg tccccccatt gttgggcaaa tatagattgg gccagagcac gaaagttaat
     1801 accacgtttg cacagctcct ccaacagcac gacaagatgc cacatactgc gccccagtcg
     1861 gtgcggttta cagactaccc gtgcgtccac cgccgataat gtcctgagca gttttttcag
     1921 tccggacctc ctgccacggt tttacgttga agaggtaacc ctgagcacgc agttcttcag
     1981 tcaggcgtgg tgcaccgtaa cgctgttatt gctgggtaag atcaaaaaac tttcaggcag
     2041 ctaaggaaag ttgaaccaga cattagatga aatatttcaa ccaattacag caccaattta
     2101 aactcagtaa atcacagcac agatcaaggg gttacgtgaa agcgtagccc ctttttcttt
     2161 ggtagtggca gcaaaatggt tgtagtgtaa aaaataatcc cgtttaatca atcaataata
     2221 catattgttt caatctacgt tattatctct ttgtaaaaat agccatttat taatcattga
     2281 aaactgcttt tagaacttga tacaacggga ctagtcacaa caggactatt ctcaacggga
     2341 tcatcctcag aggaactatc atcaaagtca tcatccataa ataaaatatc atcgaatggt
     2401 gccacgcccg tgatgagttt tattttatta ttacgatcag tcaagactcc acttaaaccg
     2461 ttttcgctca caggttttaa tgatttttca ttactcttgt tgtaagcagg cgcattaaaa
     2521 atacacggag tatcaagatc aaacaatgac gttccccagt tcacatattg aatatcatag
     2581 ttactgaagt tctgtccaga aaagaagcat cccttaaaat ccaatccacc taaattatat
     2641 aaaccatcct cttctttttg gagagtaatg ttaattctgg ctatctcccg gacaaaactt
     2701 ccttttttgt atttgaatac cgtttcaaga ttttcaccag acagcttgac ttctgggaat
     2761 aatttaaaat taaaccctac atcattatta ttcaaagtag atgaacgaaa gatggaaaaa
     2821 gcttgtagtg ctgaattata gctttcgatt ttatcttgaa gctgcgctct tggtaaaaac
     2881 ttatagcagc actcataaaa attgaataaa cgttctgaac aattcccatt tttatcaatt
     2941 aatacccccc ggaattcttt cacaaaggca tccgttattt tttgaatatc tatggtgtgt
     3001 tcctttgact ctgacaaaga tggagtctgt tgaagtgcat tgctcatctg gttaaggaac
     3061 atctgcattt ctttaaattt atctccatag tggcctttaa cttgctgaga gtttttagtt
     3121 acaggtgaaa ccttatctat ttggttgtca tataaattat gtggttccat acagacattt
     3181 ggttgtgttt ctaaaataac actacgtgta ctacctggct taaatgaatc aacatgaaaa
     3241 tcactttttc ttatattatc gaatagggtc tgttcatttc tcttagcgca ttcaataaac
     3301 tgatcaacat tatttttatt cgatgacagg tcagtttcag aaaccgcatt ttctttgttc
     3361 tccagcttta gtttaatgag aagatcttcc cagacctgct tgcttaaaca tactacgtca
     3421 ggctcaccat cagtaactaa ctcttcatta ttatcaaagt atacgttgaa tacttttaag
     3481 ttattttcat caacatcata tttaatacgt tttaattgct ctccagatcc cataatgaca
     3541 aaggcgttgc cttcatgata tatacattca gacatcattt tttgtaaagt ttcaagagca
     3601 ccgcgatacg tccctgaggc agct
//
LOCUS       DAZPEP010000272         3572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZPEP010000272 DAZPEP010000000
VERSION     DAZPEP010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.274"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..188)
                     /locus_tag="ACHUT9_004301"
     CDS             complement(<1..188)
                     /locus_tag="ACHUT9_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350615.1"
                     /translation="MTNKENKDKYINKFKKELSKYIQPTAGVDIQLFNSKDGGGVIKA
                     TLNRSGKRKSSIAGNFTK"
     gene            453..908
                     /locus_tag="ACHUT9_004302"
     CDS             453..908
                     /locus_tag="ACHUT9_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HFR1350616.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            908..1078
                     /locus_tag="ACHUT9_004303"
     CDS             908..1078
                     /locus_tag="ACHUT9_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFR1350617.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1071..1361
                     /locus_tag="ACHUT9_004304"
     CDS             1071..1361
                     /locus_tag="ACHUT9_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1350618.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1358..1720
                     /gene="rusA"
                     /locus_tag="ACHUT9_004305"
     CDS             1358..1720
                     /gene="rusA"
                     /locus_tag="ACHUT9_004305"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFR1350619.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1717..1857
                     /locus_tag="ACHUT9_004306"
     CDS             1717..1857
                     /locus_tag="ACHUT9_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFR1350620.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1943..2326
                     /gene="quuD"
                     /locus_tag="ACHUT9_004307"
     CDS             1943..2326
                     /gene="quuD"
                     /locus_tag="ACHUT9_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFR1350621.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(2515..>3572)
                     /gene="ompC"
                     /locus_tag="ACHUT9_004308"
     CDS             complement(2515..>3572)
                     /gene="ompC"
                     /locus_tag="ACHUT9_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1350622.1"
                     /translation="AVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADD
                     GDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYG
                     SIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGL
                     NFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLN
                     ASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQFDFG
                     LRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKA
                     SGVATDDIVAVGMVYQF"
BASE COUNT          969 a          797 c          857 g          949 t
ORIGIN      
        1 aatttagtaa aattaccagc aatagatgat tttctttttc cacttctatt caaagtggct
       61 ttaattacac caccaccatc cttagagtta aataattgaa tgtccactcc agcagttggt
      121 tgtatatatt ttgaaagctc tttcttgaac ttgttaatat atttatcttt gttttctttg
      181 ttggtcattg gttagatcca tagttctggc tgtgattagg tgaaagtata caaggttttt
      241 aaagcgaaga tcactgtttt ggcttcaaga tagttttgat ttatcgtaat caatccgcca
      301 taatcctctc atcggagcct gaacaactcc ggtgacttct gcgcatttaa ggggacttaa
      361 atgcgaccac aatctgaact catcaccttg tcacagatgc agaaatgcac ctgcgatttt
      421 ctgcattctg cggtttccgt tagggaggcc gtatgactct gccagtagac ggcatcaaac
      481 tccatcgcgg taacttcgcg gccatcggcc agcagattca gccattgctg gatgccgggc
      541 aatgcttacg cctgcaggtt aagccgtggc gcgagaagcg cagcctgtcg cagaacgcgc
      601 tcagccacat gtggtacacg gagatcagcg aatatctcat cgcccgcggc aagaccttcg
      661 ctacgcctga gtgggtcaaa gacgcgatga agcacaccta tctcggctac gaaagcaaag
      721 accgtgtaga tgtcgtgtcc ggcgaggtca ccaccgtcca atccctccgc catacgtcag
      781 agctggaaac cggcgagatg tacatcttcc tgtgcaaagt cgaagcctgg gcgatgaata
      841 tcggctgcca cctgacaatt ccgcagagct gtgaatacca gcagctgcgc gataagcagg
      901 aggcgtaatg gctacaccgc ttattcgtgt catgaacggg cacatctaca gagtatcaaa
      961 tcgtcgtaag cgtaagcctg agctgaagcc atccgaaata ccaacactgc tcggatatac
     1021 cgctagcctg gttgataaaa aatggttgcg actggcagca aggaggaatc atggctgatt
     1081 tgagaaaagc agcgcgtggt cgggaatgcc aggtaagaat ccctggcgta tgtaatggca
     1141 atcctgaaac gtcagtactg gcacatatcc ggctggctgg attgtgcggt accggtatca
     1201 aaccgccaga cctgattgcc accattgcat gttctgcctg tcacgacgaa atcgaccgcc
     1261 gcacatattt tgtcgatgct gagtatgcaa aagaatgcgc gctggaaggt atggcgagaa
     1321 cgcaggttat ctggctgaaa gagggggtta ttaaggcgtg aatacctaca gcatcacatt
     1381 accctggcct ccgagcaata accgctatta ccgccataat cgcgggcgca cacacatcag
     1441 cgcagaaggg caggcatacc gcgataacgt cacccgaatc attaaaaacg caatgctgga
     1501 tatcggcctg gctatgccag tgaaaatccg tattgagtgc cacatgccgg atcgccgtcg
     1561 ccgtgacctg gataatctgc aaaaagccgc ttttgacgca ctcaccaaag caggtttctg
     1621 gctggatgat gctcaggtcg ttgattaccg cgttgtgaag atgcctgtta ccaaaggtgg
     1681 gaggctggaa ctgaccatca ccgaaatggg gaatgaatga tgtttgagtt ttatatggca
     1741 gaacttcttc gccaccgctg ggggcatctg cgcttatatc gtttccccgg ttctgttttg
     1801 accgattacc gaatactgaa gaattacgcc aaaaccctga caggagcagg agtatgaagt
     1861 cagagataac aatcaactaa tactgttttg ttgatttttg cttgtaattg gcgttctggt
     1921 ctgatttttg tggagtaagt tgatgcgtga tattcagatg gttcttgagc gttggggagc
     1981 gtgggcggct aataatcatg aagatgtgac ctggtcgtcc attgccgccg gttttaaggg
     2041 attaattact tcaaaagtaa aatctcgccc gcaatgttgt gacgatgacg cgatgattat
     2101 ttgcgggtgc atggcccgtc tgaaaaagaa caacagcgat ttgcacgatt tattagtaga
     2161 ttattatgta gtcggtatga cattcatgtc actggcaggt aagcattgct gctctgatgg
     2221 ttatatcggg aaaaggttac agaaggctga gggcataatt gaagggatgt taatggcatt
     2281 agatatccgg ttagagatgg atatcgttgt taataactct aattaatatg ccaattgttt
     2341 actaaaaatt attaaaaatg gggcgttgca acgcccccaa aaataaaggg taatatataa
     2401 cagaaggttt atatagttag aagcaaggtt gtgctcctaa aggaagtggc ttgagggagc
     2461 cacttatatg ttggggaggc aaagcctccc gcaacatatc ttttagtaat caaattagaa
     2521 ctggtaaacc atacctacag caacgatatc atcggtagca acgccagatg ctttcgtgaa
     2581 atcgctctta tcaatcaggt tgattttgta atcaacaaaa gtggacatat ttttgttgaa
     2641 gtaataggtt gcacctacat caatatattc aaccaggtcc tgatcacccc acgcacccaa
     2701 gtcttttcct ttagattgca ggtaagcaac ggacggacgc agaccgaagt cgaactgata
     2761 ttgtgcaact acttcgaagt tttgtgcttt gttggcaata tggttattac caaaaacagt
     2821 catgttctgg gtttcagaat aggtggtagc cagatagatg ttgttcgcat catatttcag
     2881 accagctgcc catacttcag cattttgacc agatgcattc aggctgttgt taccgtagat
     2941 aacctgatta ttagtgcggt cagatttagc ataggttgca cctacaccga atccttcata
     3001 ctcataagta gtggagaaac cgaaaccatc accattagct tcagttacgt cagtgcggtc
     3061 atttttaccc tgatactgag cagcaaagtt cagaccatca accagaccaa agaagtcgtt
     3121 gttacgataa gttgcaacac cagtggtgcg accagtcatg aacacatctg tttgagtcca
     3181 agtgtcacca ccgaattctg gcaggacgtc agtccacgca ccgatgtcgt atgctacacc
     3241 gtagttacgg ccgtaatcga tggagccgta gtcaccgaat ttcaggccag cgaaggcaag
     3301 acgggtttta tctttggagg aaccttgaga ttcagcgcgg ttgcctttga attcatattc
     3361 ccactgaccg aaaccagtca gttgatcgtt gatttgggtt tcacctttga agccaagacg
     3421 ggcataagta gtatcaccat catctgcatc attagaggag aagtagtgct tagcattaac
     3481 tttcccgtac agatccagct tgttactgtc tttattataa atttcagctg cctgagcaga
     3541 catcgccatc agtactgatg cagctacagc ag
//
LOCUS       DAZPEP010000273         3543 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZPEP010000273 DAZPEP010000000
VERSION     DAZPEP010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.275"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..140)
                     /locus_tag="ACHUT9_004309"
     CDS             complement(<1..140)
                     /locus_tag="ACHUT9_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HFR1350623.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAV"
     gene            complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUT9_004310"
     CDS             complement(186..527)
                     /gene="yiaB"
                     /locus_tag="ACHUT9_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFR1350624.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHUT9_004311"
     CDS             complement(696..2150)
                     /gene="xylB"
                     /locus_tag="ACHUT9_004311"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFR1350625.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUT9_004312"
     CDS             complement(2222..>3543)
                     /gene="xylA"
                     /locus_tag="ACHUT9_004312"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFR1350626.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          925 a          982 c          783 g          853 t
ORIGIN      
        1 acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag ccctaacaga
       61 taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc cggtgaatag
      121 gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta agagctttat
      181 gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa aaaggcgatg
      241 gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat aaacaagaga
      301 ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc cagttgcccg
      361 agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa cacgccaaga
      421 aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag ataaaccagc
      481 gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac gatctccata
      541 tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat gccattaaca
      601 aatgattttc agaataaatt catactaaat attaattaat tagtgagata tatagatgtg
      661 aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg cagaagttgc
      721 tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg ctgcgcatct
      781 ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga tttctctgga
      841 ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc cccccccgta
      901 cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta ctcactacgc
      961 gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg cacgacatcc
     1021 atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag ttcattgggg
     1081 ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt gtgtggtgta
     1141 cgctcgccgg aaagataagg cagaaaccaa actggctcga cactttcatc agcctgttga
     1201 gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc ccaatccaga
     1261 cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag cgcatggcaa
     1321 aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc aaaatagacc
     1381 cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac accaactgca
     1441 ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc ccacgctttc
     1501 gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc gggcatctgg
     1561 tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt tgcgacatcc
     1621 agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt catacgcaga
     1681 cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc cggctcatgc
     1741 cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt gccggtaatc
     1801 actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca gcgcccgtcg
     1861 ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt tgctccatgc
     1921 atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc gcccagagct
     1981 ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga ccagagtgga
     2041 tgcgggcgcg aaacggtcag cttttccgtt tgcgaatcaa ccacctcacc ctgctcgttg
     2101 agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat atcgatcgtt
     2161 ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc acagttagcc
     2221 gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg cgaccactct
     2281 gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc agtgacattt
     2341 ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc tgcgcgatgc
     2401 gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc agtgccatcg
     2461 tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt tgacgacgta
     2521 ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga atttcataca
     2581 tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag cccagttgcg
     2641 catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg gtggctattt
     2701 catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc agtttaatct
     2761 ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg taatcatatt
     2821 gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctgg aagccgattt
     2881 tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc tcctgacgca
     2941 agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca tagttttcac
     3001 cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc cagctgaaga
     3061 cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta aagcagttag
     3121 cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg acatcaacca
     3181 tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa acatccacat
     3241 cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg acatctgctt
     3301 tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta aacgccccca
     3361 caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg gcaaaacgca
     3421 agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga cggaatgcta
     3481 acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca aaataggctt
     3541 gca
//
LOCUS       DAZPEP010000274         3533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZPEP010000274 DAZPEP010000000
VERSION     DAZPEP010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.276"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..638)
                     /locus_tag="ACHUT9_004313"
     CDS             complement(<1..638)
                     /locus_tag="ACHUT9_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1350627.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLADAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTL"
     gene            complement(645..953)
                     /locus_tag="ACHUT9_004314"
                     /pseudo
     CDS             complement(645..953)
                     /locus_tag="ACHUT9_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
     gene            complement(1332..1844)
                     /locus_tag="ACHUT9_004315"
                     /pseudo
     CDS             complement(1332..1844)
                     /locus_tag="ACHUT9_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdaU family protein"
     gene            complement(1857..2279)
                     /locus_tag="ACHUT9_004316"
     CDS             complement(1857..2279)
                     /locus_tag="ACHUT9_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFR1350628.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVIVH"
     gene            complement(2276..2518)
                     /locus_tag="ACHUT9_004317"
     CDS             complement(2276..2518)
                     /locus_tag="ACHUT9_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1350629.1"
                     /translation="MNKISTYRKQLGLSQRQLAVQLGWIQSRLANYEANFRTPGLEEC
                     RKIVSTLNRLGAHCGLDDVFPPDGKHSENSIGAVDS"
     gene            2615..3034
                     /locus_tag="ACHUT9_004318"
     CDS             2615..3034
                     /locus_tag="ACHUT9_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1350630.1"
                     /translation="MEKENEKIAASRLNDKIAMRLKERRQKLGLSQGKLAEICGWTQS
                     RIGNYEAGSRNVGVHDAVVLGKALGISPPELLFGEQESSELWLNESQRKLLELFNQLP
                     GSEQQRMIELFEVRLKEIDEYVEKYLRGRIKDNPPPE"
     gene            3340..3495
                     /locus_tag="ACHUT9_004319"
     CDS             3340..3495
                     /locus_tag="ACHUT9_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1350631.1"
                     /translation="MNTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
BASE COUNT          893 a          928 c          794 g          918 t
ORIGIN      
        1 agtgtattca tcgctgcatg gttaatattt gtcagcatcc ccacaccgcg taacgatgcc
       61 gtccggcgat caacaatctg gttcagtacc acctgctcgt ttttcgtatc ccgctgcatg
      121 ccaatttcat ccaggaccag caggtcaaca ccacaaagct cctgtaaaaa tttttcaccg
      181 gatttgccgt tgtcgtagct ctcatgcaac acgctcatga catcggacac ggtgacgata
      241 atcacgctac gccccttcgc catcagccgg ttgccaatcg cgtctgcaag atgatttttt
      301 ccggtgccgg gcttaccgct gaacacaaaa ttcgtacagc ctgtcatcag ttcgtcagct
      361 atggatttcg cctgactcag tgcatgtcgc tgaccgtcgt tctgcgcccg gtaattcgca
      421 aacgaacact tccggtgcaa cggctggatg ccggagcggt tcaggatttt ttccacccgc
      481 aactgacgat tcaggcggtt gatctcctcg caacgtttct ggccttcagc aagttgccac
      541 tcgcgccact ccgcaaccgt tctgaatggg gcggttacat gtggtggggt cagtctgcgg
      601 atacgctcca gaacgcctcc tgtcgcaata tttttcatgg tctgttaccc cctgaagcct
      661 ggcgggatcg cactgtccgg caacgagacg gtgttaacct gtcggagcaa cgtctcaggc
      721 cgaacacctt tcggcgcgaa cagaccctgg tattcattgg cgatgctgtg tcgaatcacc
      781 tgctcaggtg taaaaccctg ctgacggaat ttttccagtt cccgtatcgc cccgttagcg
      841 ccctgctccg ttcgaatcgg ttttcgcaat gcctgcctga actggaccca ctcatgccag
      901 agtgtttctg gcaaccagtc gggcagatca actgacaacg ggtcaaattt tttctggcga
      961 acagccccac cgataaccag aattccctgc tcggtgtgcc ttgcgatatc actgcgccgc
     1021 aagacacata tcccggtttc aacagcaccg attttgagca gtgatttctg tcgcggagaa
     1081 tttcggtcaa gctcgctccc acaccggaca ccgttcctgt cagtcacgac gatatcaatc
     1141 cgccctccgc aaccgtcacc acgctctggc accggatact cacgacagac atccaggcca
     1201 gcagcctgca acgcaaccac cgcagaatta caaaattccg ctgcagtacc gccagataat
     1261 ctcccctcaa gcactccagc cacaaaatcc cgaaactccc ttccggaagg gaagggtttg
     1321 ggatgggtta gatctgtttt tagatcttta tctgtatctt tattagttgc ctttgtgttg
     1381 gcatcatgtt cgaacatcac tccaacatct gtttgaacac ctgttaaatt tctctcttgt
     1441 tttgtttgaa catctgcttc ctttctgctt cttctggcct gaacagatgc ttttcctgcg
     1501 gctgattttt tggttaattt ttctctgaca gatgccagat cttcctcaat ccgaagatgc
     1561 atccattcgt cgccgttatc acaaaaaaac tcccgcaagg atggttcaac atcaacccat
     1621 cgctcgttag tcagacgggc aatttttgcc aacctgtttt tgggtattgg ctttcctgtt
     1681 tgccagtaat tgaacatcag caacaaatac gcgccgtgct cctctgctga caaatgcatg
     1741 gtgtcagcca ggtaatcagc tatgtacagt tgcatgtatg gtaatgcggc cataattgcc
     1801 ccgtatgatg ctgcccggtt gcttagaata agcacaaaca gcatggaaac ttttgcttaa
     1861 tgaacaatga cagaatcgtc ggaagacccg ccgccgctga aatgcgcttt ccggtaaacg
     1921 gcctggactg catcatcatg cgcatcaatt gccgtactca acgcttcctg cgccgccagt
     1981 aatgcacggc gttccagggt atcgaagatg cagagtcggt gacgcagctc gcgcggaaga
     2041 attgccagaa ccgcagggat cagtttctga attttttccc tttgcgcttt cgtttcacct
     2101 ttcaaccaac ggtgatagat attctgctga ttgttccagt ccttgcctgg tacaaggggc
     2161 aattcgccgc ccccctggcg cagatattct tcagtaattg cgttagcgac ccacgcctgc
     2221 cctttttcgg ctgccagggc taacaacact gattcgatgt gctcatgcct gattttcatg
     2281 aatcaaccgc tcctatgctg ttttcgctat gcttaccgtc tggggggaat acatcgtcaa
     2341 gtccacaatg agcgccaagc cgattaaggg tagaaacaat ttttctgcac tcctctagtc
     2401 caggggtacg aaaatttgct tcgtaatttg ccagtcggct ttgtatccac cctaactgaa
     2461 cagcaagttg tctttgagac agcccaagct gttttcgata tgttgaaatt ttgttcattg
     2521 aaaacctccg atgacaattt taaacacacc ttgtgttata tagccaagct gttttgtgtt
     2581 ttatgtaaat cacgattcgt gatacaagga tgcaatggaa aaagaaaacg aaaaaattgc
     2641 cgctagtagg ctcaatgaca aaattgcaat gcgtcttaaa gagcgcaggc agaagcttgg
     2701 tttatctcaa ggaaaacttg ctgaaatctg cggatggacg caatcgcgta taggtaacta
     2761 tgaggcgggc agcagaaatg ttggagtaca tgacgctgtc gtattgggaa aggcacttgg
     2821 catatctcct cctgagctcc tctttggaga acaggaatct tctgaattgt ggttaaatga
     2881 atcccaacga aaacttcttg agttgtttaa ccagctaccg ggctcagaac aacaacgaat
     2941 gattgagcta tttgaagtcc ggctaaaaga aatcgatgag tatgtagaaa aatatttgag
     3001 aggcaggatt aaagataatc ccccaccgga ataatgatct tgctatcaca gtaatatgcc
     3061 aatcagcccg ctatcagcgg gcttttttgt agcatcatca tatgacactc accataaaac
     3121 acattgcgtg ttgacacaag aaaacaaatt gtgtttaata agcatatcca aacaacgccc
     3181 caccagagaa cggcaggaca atacctcgag ttatccagtc actgaacagg gctaagtagc
     3241 cagcctgagg catacgaaca tgacggcagt tgttgattga tacaaagcgc agtagataaa
     3301 acgttccgcc acccggcgtt aaggggaaat ggggtcaaca tgaatactat cgatcttggc
     3361 aacaacgaat ctctggtatg tggcgtgttt cccaatcaag acggcacatt caccgccatg
     3421 acgtatacca aaagcaaaac gtttaaaacc gaagctggcg cacgtcgttg gttgggaaga
     3481 cattcaggtg agtaaaatga acgagacaga attaaaacac gttatcgctc tac
//
LOCUS       DAZPEP010000275         3440 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZPEP010000275 DAZPEP010000000
VERSION     DAZPEP010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.277"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="ACHUT9_004320"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="ACHUT9_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="ACHUT9_004321"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="ACHUT9_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFR1350632.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHUT9_004322"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="ACHUT9_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFR1350633.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3440)
                     /locus_tag="ACHUT9_004323"
     CDS             complement(3352..>3440)
                     /locus_tag="ACHUT9_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="activates the production of the major fimbrae
                     protein FimA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbriae biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR1350634.1"
                     /translation="AHKSNIYGKLGLHSIVELIDYAKLYELI"
BASE COUNT          860 a          768 c          879 g          933 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcctggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctggctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccacccgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttcagttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc tcgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac
//
LOCUS       DAZPEP010000276         3343 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZPEP010000276 DAZPEP010000000
VERSION     DAZPEP010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.278"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(342..417)
                     /locus_tag="ACHUT9_004324"
     tRNA            complement(342..417)
                     /locus_tag="ACHUT9_004324"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Asn,seq:gtt)
     gene            complement(518..2005)
                     /gene="yeeO"
                     /locus_tag="ACHUT9_004325"
     CDS             complement(518..2005)
                     /gene="yeeO"
                     /locus_tag="ACHUT9_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFR1350635.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            2166..2241
                     /locus_tag="ACHUT9_004326"
     tRNA            2166..2241
                     /locus_tag="ACHUT9_004326"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Asn,seq:gtt)
     gene            complement(2279..3229)
                     /gene="cbl"
                     /locus_tag="ACHUT9_004327"
     CDS             complement(2279..3229)
                     /gene="cbl"
                     /locus_tag="ACHUT9_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFR1350636.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          983 a          865 c          701 g          794 t
ORIGIN      
        1 gaaataatat ttattaaaat tccagttctt ctttttctga ttacagaagg caaagtggca
       61 attacgcata gtttcccgat aaagacgcga tagcgacatc ccgcataagg catttttctc
      121 tttatctttg tacggtactt catggaacag agtttttgac cttgcgaatc gtgatgtctg
      181 ttggggaggg gcaatttgct cactgaagcg tgagactcga ttaagcgcac gaaacacaga
      241 aatcaaaaaa cccggtcact tttttacaag gtaaccgggt aaaaataatt tttatttttt
      301 aactgttttg agactcatag agatgtctca aaactaaaat ttggctcctc tgactggact
      361 cgaaccagtg acatacggat taacagtccg ccgttctacc gactgaacta cagaggaatc
      421 gtgagaacga ggcgaatatt agcgatgccc acccacaatg tcaaagcctg ttttttaaat
      481 ttgaaatcgt ttgctgaaat aatctgcatt ttgtcgttta ttccgacaca actggctttt
      541 tttcacactt ttgcggttcg gatcgagggt atttccacag ccaacgtcca gtaaccattc
      601 gccagtaaaa cagcacggcc cgcacagccc agtcggcaaa cattcccatc cagacaccaa
      661 ccacacccca gccaagcatg atgcccagca cataaccgac gacaacccgg caaccccaca
      721 tgctcaacat agaaacccac atggcgtaac gggcatcgcg agcaccttta aatccagcgg
      781 gtagcaccca tgaggcggac caaataggca taaataaagc atttagccaa atcagaatca
      841 caacgacatg tttaacctgt ggatcctggg tgtaaaacga tgccataacc ccggcaaagg
      901 gagccgttag ccaggcgatg gccgttaatc caagagtgga aagccagaac acatgccgca
      961 actgaatctc tgcttgcgct atctgcccta cccccaacct tcggcctgta atgatcgtag
     1021 aagcagagcc gagcgcactt ccgggtaagt tgataagagc cgcaattgaa aacgcgataa
     1081 aatttccggc aataacactg gtccccatcc cggcaacgaa catttgggtt aataaccgac
     1141 cactggtaaa taacactgat tcgacactcg cgggaatacc aatccccatg acttcccaga
     1201 taatgctaaa attcagcggt ttaaaatagc tctttaacga aatccttagc gcaggattaa
     1261 aaccaatcgc cagcacccac aaaattgcaa ctgcgccaat ataacgagaa atggttaaac
     1321 ccagccctgc cccgacaaat cccagtcccg gccaggagaa aaggccgtaa atcaatatgc
     1381 cgctaataat aatattaaga atattcaggc taccgttaat caatagcggt attttcgtat
     1441 tccctgcacc acgaagtgcc ccgctaccaa taagagtgat ggcagctgct ggataactga
     1501 gtaccgtcag ctccagataa gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg
     1561 cgacgaaatc aataatttgt tcgccaaaat gatgaataag cgttgccaac agtacggcaa
     1621 acaacgtcat gatcaccaat gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct
     1681 taccgagact aaatgccaca acgacagtag taccaagatc gatagcagca aaaaaagcca
     1741 taatgaccat attgaagctg tccgccaatc ccacgccggc catcgcatct tttcccagcc
     1801 agctgaccag aaaagtgctc agaaccccca tcaacaggac acaggcattc tccatgaaga
     1861 taggaacagc aagcggggtt atctcgcgcc agaacaacac tttgtagctc ttgcgtttag
     1921 cgtgccagcg agtgccgtga acaacctggc gtaaagcaga ggagatattc aaagccgacc
     1981 ttaattgcag aaagtgaaac cacatttcaa ataatgaggg agaatcagca aagctgcaaa
     2041 gattttcgcc aacaaattgt ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt
     2101 tggggaattt aaaaatcggg tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca
     2161 gcgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     2221 cgagtccagt cagaggagcc aaatttctga aaagcccgct tttatagcgg gctttttgct
     2281 atatctgata atcaatttcc tcttcactgt tttccatcac ctgccgcttg atatcctcaa
     2341 ctgacagtcc tgcattacaa agttccagaa agcgccagac atagttacgc tgaagttgtc
     2401 ctcgcttcag tcccaaccag acagtattag catcgaaaag atgccgcgta tccagacgga
     2461 ttaaattctc ttcctcttgt tcgccgctgg attgttcggc aactaatccg atcccaagcc
     2521 caagagcaac ataggtttta atgacatcag aatcctgcgc acttaataca atatctgcca
     2581 gcaaaccttt gcgggcaaat gcgtcatcaa tacgtgagcg ccccgtaatc ccctgtcggt
     2641 aagtgattaa cggccacttc gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg
     2701 gatgatcgag tggaacaagc aaactatggt gccaacgaaa ccacgggaag gcgacgagct
     2761 gcgggtcatt actcaaacgc tcgctggcga taccaatatc agcttcgcca ttttgcaaca
     2821 acgtcgcaat ttcctgtggc gtcccctgga ttagctcgag ccgaacctcc gggaaaagtt
     2881 cgcgaaaagc tttaatgacc tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa
     2941 tagtgagaac gccagacgta tcgttggtaa acaggtctgc aagccgacga acattactgg
     3001 cttcattcag aatacgttct gcaatgacca gtaatgcttt gcccggttca gtcatgccca
     3061 gcagtcgctt acctcgtcga acaaatattt cgatgccaag ttcatcctcc agttcccgaa
     3121 tatgacggct gacgccggac tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt
     3181 aatcctgacg tgcagcctcg cggattatct ttagttgttg gaaattcacg gtaaactccg
     3241 ggcagttcag atttcccgtt attgttaaag tctaatgccc ggcataacaa ataataaaaa
     3301 cccgcatctt attccatccc gatataacgc ttagctcacc aat
//
LOCUS       DAZPEP010000277         3322 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZPEP010000277 DAZPEP010000000
VERSION     DAZPEP010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.279"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHUT9_004328"
     CDS             complement(<1..320)
                     /gene="fliZ"
                     /locus_tag="ACHUT9_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFR1350637.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHUT9_004329"
     CDS             complement(366..1085)
                     /gene="fliA"
                     /locus_tag="ACHUT9_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFR1350638.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1250..2824)
                     /locus_tag="ACHUT9_004330"
     CDS             complement(1250..2824)
                     /locus_tag="ACHUT9_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFR1350639.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3079..3322
                     /locus_tag="ACHUT9_004331"
                     /pseudo
     CDS             3079..3322
                     /locus_tag="ACHUT9_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
BASE COUNT          852 a          789 c          713 g          968 t
ORIGIN      
        1 accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc gataatatcg
       61 aaaaaatcac tctgcgtttt gcagtgtaaa tcaccacaaa atcggcacaa tgccgcccat
      121 gatttttgtt ccacttgcca gccattttca tcaagcagca tgtccaggcg ggaaatctcg
      181 attttattca ctattttgcc gtcgcgaacg agggtaatgc ggtcgagtaa tttacggcaa
      241 tgcgcgcaat gagtctggct gtgtttaaag tctttaagat agcggcttaa tggccgtctt
      301 ttcaggtgct gcaccatcat taagaactcc tggtagtcaa aattaaagtg cggcatttac
      361 tgacgttata acttacccag tttagtgcgt aaccgtttaa tggcctggct gtgtaactga
      421 ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt cagctcttcc
      481 tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat ggcttccatc
      541 acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg ctgatgatca
      601 tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta ggagaagagc
      661 tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat ccctaaacgt
      721 tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg ccctattgcc
      781 tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg gctgcgaagt
      841 tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc cgttccttgt
      901 agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg tagcagatcg
      961 tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg acgcaccagc
     1021 gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt atagagtgaa
     1081 ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt ttctgcaggt
     1141 tttttaatcg gacgattagt gggtgaaatg aggggttatt taggggttac cggtaaattg
     1201 cggacagaaa aaaccccgcc ggtggcgggg tttgagcgat aagtgtaaat taacgcagca
     1261 gagacagtac gttctgcgtg gtctggttag cctgtgccag aacagaggta cccgcttgtt
     1321 gcaggatctg cgcacgagac atgttagaca cttcggttgc gtagtcagca tcttctatac
     1381 ggctacgggc agaagacagg tcgtttacgg tgttgccaag gttggtgatg gcagagtcga
     1441 aacggttttg tactgcaccc aaagaactgc gcaacttatc aacttttgcc aaagcttcgt
     1501 ctaatttgga taatggattt gtggttgcta aggctttcaa atctgaggca ctgagttcag
     1561 cacctttggt tgccgtctta ttcgtgatat tggctgtata aaccttatca tcttcacgca
     1621 taattgcctg tgcagaacca tcatttttta ctgcccattc aactgcctta ccattaggat
     1681 cagtaataac agacgtgtta cctgtagcgg catctacatt tactgcaatg taggtgccgt
     1741 ctgatgtttt agcagcaaat gaattaggta caggattccc ttgggtatct tgtacttggt
     1801 ggacatctag gttttttccc ccaaaataaa cagacattgg tgcatcatta taagctaatg
     1861 ctttacccat tttacccgca gctaaatcaa ctgatgtggc agaaccatta tcttcaacaa
     1921 atgcagttac acgaccacca aagtttgcta actgaacatc cttaccaccc acctgaactt
     1981 tttcataacc tgtagagttt tgtttcaata atgtaaaacc gttcgtgttg gtattattat
     2041 cttgaccaac aaaataagtc tgcccttcca cctgtacttg ataaactttt gttcctgcag
     2101 taccggtcgc atcagttgta tcctttgtat cttgtacatt accggtacca aaataagttt
     2161 caacatcagc atttttataa ttgctaccga atgctttagc acctcccaca ctaccatcag
     2221 ttgcatttac cttagttaag tcaggagcgg cttcgctctt aagtgcaacc gcagaggaca
     2281 ttggaacttt tcctagtgct acgctaaagt tatcaagccc caaggtttta gcatcaattt
     2341 tatcaagggc aatctcaatg gtttcaccat cgttcgcacc tacttgaatt ttcatagtct
     2401 gatcagaagc cagaactttt acaccattaa attgtgtctg gttagaaaca cgatcaattt
     2461 cactaaggcg ttgtgtaatt tcatcctgaa ttgaattcag gtcggagtca gagtttgtac
     2521 cattagtggc ctgtactgac aattcacgaa tacgctgtaa gttattgttg atttcagaaa
     2581 gcgcaccttc agtggtctgc gcaacagaaa taccatcatt cgcgttacgg gaagcctgag
     2641 tcagaccttt aatatttgct gtaaaacggt tagcaatcgc ctgacctgct gcgtcatctt
     2701 tagcactgtt aatacgcagg ccagaagaga gacgttcaat ggcggagctc agagaagact
     2761 gagatttgtt caggttattc tgggtcaaca gcgacaggct gtttgtatta atgacttgtg
     2821 ccatgatctt ttccttatca attacaactt gatgttattg ggctgttgcc cacggtttct
     2881 caccgtaccc cttgtatcgg cacctgaatt tcgaacttta gaaaatttta gtttctctcc
     2941 tgttttttct taacggctga aatatccgta atatacaact ctactctgcg catcatcttc
     3001 gtgaatcaac tattaaactt tgcgcacaaa ttgccgataa cccgcttatc tactgttttc
     3061 aatcaaaagg aattaggtat ggcttcattt acatctcttg gcgttggttc gaatctgccg
     3121 ctggatacat tgctaaacaa cctgaccatc gctgaaaaaa aacgcttaaa cccaattact
     3181 cagcaacaga gtgacaatac cgcacgtctg acagcctatg gcacattaaa aatgtgcatt
     3241 agaaaaattc cagactgcaa atactgcgtt aaataaagcc gatctgttca aaagcactac
     3301 cgttaccagt agcaatgaag ac
//
LOCUS       DAZPEP010000278         3224 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZPEP010000278 DAZPEP010000000
VERSION     DAZPEP010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.280"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHUT9_004332"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="ACHUT9_004332"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFR1350640.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHUT9_004333"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="ACHUT9_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFR1350641.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHUT9_004334"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="ACHUT9_004334"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFR1350642.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHUT9_004335"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="ACHUT9_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFR1350643.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          755 a          864 c          770 g          835 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtct
//
LOCUS       DAZPEP010000279         3216 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZPEP010000279 DAZPEP010000000
VERSION     DAZPEP010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.281"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..495
                     /locus_tag="ACHUT9_004336"
     CDS             <1..495
                     /locus_tag="ACHUT9_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFR1350644.1"
                     /translation="SRAPFIITPPLFRIEAGDDSSLRIIKTADNLPENKESLFYINVR
                     AIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRSDHSLNIYNP
                     TEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAINDYGGSSGTET
                     RPLQ"
     gene            525..3041
                     /locus_tag="ACHUT9_004337"
     CDS             525..3041
                     /locus_tag="ACHUT9_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1350645.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3052..>3216
                     /locus_tag="ACHUT9_004338"
     CDS             3052..>3216
                     /locus_tag="ACHUT9_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1350646.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          939 a          771 c          730 g          776 t
ORIGIN      
        1 agccgtgccc catttattat aaccccaccg ctatttcgta ttgaagctgg cgatgactca
       61 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      121 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      181 acgctggtat ttaaaacacg gatcaaaatg ttttatcgcc ccgcacacct gaagggacgg
      241 gtaaacgatg cgtggacatc actggaattt aaacgtagtg accattcact caatatatat
      301 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
      361 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc tgcatctggc
      421 ggaaagaacg tgaaatgggc tgcaatcaat gattatggcg gcagttccgg gacagaaact
      481 cgtccactgc aataacaaat ataaaaaaca caggtcatca gggaatgcca caacgacacc
      541 accagggaca taaacgcaca ccgaaacagt tggcgctcat catcaaacgt tgtttgccga
      601 tggtgctcac tggcagcggc atgctttgca ctaccgctaa cgccgaagag tattatttcg
      661 accccattat gctggaaact acaaaaagtg gtatgcaaac aaccgatctg tcacgttttt
      721 caaaaaaata cgcacaacta ccaggaactt atcaggttga tatctggctg aataaaaaga
      781 aggtttcaca gaaaaaaatt acatttaccg ccaatgcaga gcaacttctg cagccacagt
      841 ttacggtaga acaactacgt gagctgggta ttaaggtgga tgaaatcccg gcgctggctg
      901 aaaaagatga cgatagcgtg atcaactcgc ttgaacaaat cattcccggt acagcagctg
      961 aatttgattt caatcatcag cgacttaatt tgagcattcc ccaaattgca ctgtaccgtg
     1021 atgcaagagg ttacgtctcc ccttctcgtt gggacgatgg tataccaacg ctgtttacca
     1081 actactcgtt tacaggttct gataaccgtt accgccaggg caatcgtagc caacgacagt
     1141 acctgaatat gcaaaatggt gctaattttg gcccctggcg attacgcaac tattccacat
     1201 ggacacgcaa cgatcagaca tcaagctgga ataccatcag tagttattta caacgtgata
     1261 tcaaggcatt gaagtctcag ttgcttctgg gagaaagcgc caccagcggc agtatttttt
     1321 ccagctacac ctttactggc gttcaactcg cttccgacga taatatgttg ccaaacagcc
     1381 agcgcggatt tgccccaacg gtacgcggta tcgcaaacag tagtgcaatc gtgactatca
     1441 ggcaaaatgg ttatgtgatc tatcaaagca acgtgccagc gggtgccttt gaaattaacg
     1501 atctctaccc ctcttccaac agcggcgatt tagaagtcac gattgaagaa agtgacggta
     1561 cgcaacgtcg ctttatccag ccttattctt cattacccat gatgcagcga cctgggcatc
     1621 taaaatatag cgcgaccgct ggacgctatc gcgctgatgc aaacagtgat agcaaagaac
     1681 ccgaatttgc tgaagccacg gcaatatatg gtttgaataa tacttttacg ctgtatggtg
     1741 gcctgctcgg ttctgaagat tattatgcgc tggggatcgg tatcggcggc acacttggcg
     1801 cactgggcgc gttgtcgatg gatatcaaca gagctgacac ccaattcgat aaccagcact
     1861 cttttcatgg ctatcaatgg cgtacgcagt acatcaaaga tatcccggaa accaacacca
     1921 atatcgctgt cagctactat cgctatacca acgatggcta ttttagtttt gatgaagcca
     1981 atacccgtaa ttggaactat aacagtcgcc aaaaaagtga aattcaattc aacatcagcc
     2041 agacaatatt tgatggggta agtctgtatg cctccggttc gcagcaagac tattggggca
     2101 ataacgataa aaacaggaat atctcagttg gggtttccgg tcagcaatgg ggaattggtt
     2161 acagcctgaa ttatcaatac agtcgctaca cggatcaaaa taatgaccgc gcactctctt
     2221 tgaatctcag tattccgtta gaacgctggt taccgcgtag ccgggtttcc tatcagatga
     2281 ccagccagaa agatcgccca acccaacatg aaatgcgtct taatggctca ctgctggatg
     2341 atggtcgcct gagctatagc ctggaacaaa gtctggatga cgataacaac cataacagta
     2401 gcctgaacgc cagttaccgt tcaccttatg gcaccttcag tgccggatac agctacggta
     2461 atgacagcag ccaatacaat tacggcgtta ccggcggcgt ggttatccat actcatggcg
     2521 tgacgctctc gcaatatctg ggcaacgctt ttgcgcttat cgatgctaat ggggcttctg
     2581 gcgtgaggat acaaaactat ccggggattg ctactgatcc ctttggctat gcagtggttc
     2641 cttatctcac gacttaccag gaaaaccgtc tctcggtaga tactacgcag ctgcccgata
     2701 acgtcgatct tgagcaaaca acacagtttg tggtgcccaa cagaggtgca atggtagcgg
     2761 cgcgtttcaa cgccaatatc ggttatcgcg tacttgttac agtcagcgat cgcaacggta
     2821 aaccgttgcc ctttggcgct cttgccagca acgatgagac gggacaacaa agtatcgtcg
     2881 atgagggcgg catactatat ctctctggga tatcgagtaa atcacaaagc tggactgtac
     2941 gctggggaaa tcaggcagat caacaatgtc agtttgcttt tagtacaccg gattcagaac
     3001 caacaacctc tgtattacaa ggcacggcgc agtgccatta aggataaaaa aatgaaaaga
     3061 gcgcctctta taacaggact tttgttgata tccacatcct gcgcttatgc ctcctcagga
     3121 gggtgtggag ctgacagcac tagcggtgcg acaaattaca gcagtgtggt tgatgatgtt
     3181 acggtgaacc agacagataa cgtgacagga cgggag
//
LOCUS       DAZPEP010000280         3185 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZPEP010000280 DAZPEP010000000
VERSION     DAZPEP010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3185
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.282"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUT9_004339"
     CDS             complement(9..635)
                     /gene="dsbA"
                     /locus_tag="ACHUT9_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFR1350647.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUT9_004340"
     CDS             complement(652..1638)
                     /gene="srkA"
                     /locus_tag="ACHUT9_004340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFR1350648.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(1715..1984)
                     /locus_tag="ACHUT9_004341"
     CDS             complement(1715..1984)
                     /locus_tag="ACHUT9_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFR1350649.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUT9_004342"
     CDS             2054..2638
                     /gene="mobA"
                     /locus_tag="ACHUT9_004342"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFR1350650.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUT9_004343"
     CDS             2620..3147
                     /gene="mobB"
                     /locus_tag="ACHUT9_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFR1350651.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT          832 a          772 c          769 g          812 t
ORIGIN      
        1 acattgaatt attttttctc ggacagatat ttcactgtat cagcatactg ctgaacaaaa
       61 acatccatat tgctggtatc cataccctgc ggattcagct gatatttacc gttaacaaac
      121 atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt tttcctgctg agcgaccaga
      181 gatttgacca cgaagctgtt ccacgccgcg tcgtactctt cacctttaat acctgcgttg
      241 ataaatacat cgcggatatc agatgctgaa cgaatggtct gggttttctg tacgccttca
      301 aacagcggaa cagtgacttt gtcttccacg cccagcgcca tcgccacagc ccatgcctga
      361 gtcagatctt tgcccaggtc accacccatg aagttgacgt ggtatttagt catcttcacg
      421 ccttccggca gttttttctt cacattatca gaaatatgca gaacttcttc aaactgatag
      481 cagtgcggac agaagaaaga gaaaaactcc agcacttgcg gcgcgccagc taccggtttt
      541 tccagggtag tgtactgttt accatcttca tactgcgccg ccgatgcgct aaacgctaaa
      601 actaaaccag ccagcgccag ccaaatcttt ttcatgatct actctctccg attaatacat
      661 aggtgttaat tgtaaagggg gttcttgtag aacttttgcc tgttctataa aagtcgccgt
      721 ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa tttttcggga acgcgggatc
      781 agcccaacgc cgcattagcc aggcaagata ataaaccaaa cgcatggcgc gtaaaggttc
      841 aatcagtccg atttcagcgg tgtcgaactc gccaaattct tcataagctt caataatagt
      901 ttccagttgc atccgctgct cggctttatc gccattgagc aacatccaca aatcctgaat
      961 agctggacca ttacgtgcat catccagatc aacaaacatt ggaccatcgc gccagagaat
     1021 attcccggcg tggcagtctc catgtagccg cagaacggtg aaatcttccc gccagtgtgc
     1081 tgtaacggcg gcgatcagct catctgtcgc tttcaggaat gccgctttca acccggaagg
     1141 tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg agatattcat tcagaccgat
     1201 ggtcgggcga tggataaaaa gctgtttgtg ccccgtctgg tgcatacgcc ctaaataacg
     1261 cccaaccgct tccgtctgat cgatattatc agcttcgaac tggcgaccac cgacgcttgg
     1321 aaaaacagcg aaataaaatc cctgatgatt caataaagtc tgaccgttaa aggccacagg
     1381 tgctgcgacc ggaacttcat catttaccag ctgcaacgca aattgatgtt cttcgaggat
     1441 ttgatcggct gtccaacgtt cagggcgata aaatttgacg acaaaacgtc gacgctcttc
     1501 gtcctgaaat tgatagacac ggttttcata gctgttaagc ggggtaagac cggaatccac
     1561 ccggatccct tgctcaaaca gagcgtccat gatggtatcc gggtgtagtg tctggaaagt
     1621 aaaagcgctg ttgttcatcc catcatccgg aaaacattac gaatgatgaa ggatatcatt
     1681 tcacgatgat tttggtggcg ccgcttacaa gcttttactc tttaattacg ccgcgagcgc
     1741 gtaacagcgc cgttttgaag tcttcttcat aatctttctg caaacccggg atcaccgcat
     1801 ctttcgcgga atcacgcatt ttcaggtgat agatcagaat gtcatccgtc aaatccgcca
     1861 gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa aaattgcaac aggttaaggt
     1921 ctggctcttt ttgccaggct ggctggagga gttcaataac ttcattcaga cgtttacatt
     1981 tcatagtgat gctccttact cttgagacag acacgttagc agggtcaatc ccacaataaa
     2041 agaggcgata tcggtgaatc tgatgacgac gataacaggc gttgtgctgg caggcggtaa
     2101 agccagacga atgggcggcg tagataaagg attgcttgaa ttaaacggca aaccattatg
     2161 gcaacatgtc gctgacgcgc ttatgacgca gctctctcac gtcgtggtta atgccaatcg
     2221 tcatcaggaa atctatcagg caagcggtct gaaagtgatt gaagattcac tggcggatta
     2281 cccaggccct ctggcaggaa tgctttcagt aatgcagcag gaagctggtg agtggttttt
     2341 gttttgtccg tgcgatacgc cttacattcc ccatgtttta gcttcccggc ttaatcatca
     2401 gcgcaaagat gcgcctgtcg tgtgggtcca tgacggtgaa cgcgatcacc cgactattgc
     2461 tctggtaaac cgtgctattg agcctttatt actagaatat ctgcaagcag gagaacgccg
     2521 ggtaatggca tttatgcgtc tggctggcgg ccatgcggtt gatttcagcg atcataaaga
     2581 tgcatttgtt aacgtgaata cgccagagga gcttgcccga tggcaggaaa aacgatgata
     2641 ccgttactcg cctttgccgc gtggagtggt accggaaaaa ctacgctttt gaaaaaactg
     2701 atcccggcat tatgcgccag agggatccgt ccagggctga ttaagcatac gcaccatgat
     2761 atggatgttg ataagccagg caaagatagc tatgagctgc gcaaggctgg cgcggcacaa
     2821 accatcgttg ccagccagca acgatgggcc ttgatgacgg aaaaaccaga cgaagaagag
     2881 ctggatctac agtttctcgc aagtcgaatg gatacctcaa agctggattt gattctggtc
     2941 gaagggttta agcatgaaga gatcgcaaag attgtgctgt ttcgcgatgg agccggacat
     3001 cgacctgaag aattagtgat agacaggcat gttattgctg tagccagtga tgtgccgctt
     3061 aatcttgatg tcgcattact ggatattaat gatgttgagg ggctggctga ttttgtggtg
     3121 gagtggatgc aaaagcagaa cggataagca agagtaggct ggataagacg cgccagtgtc
     3181 gcatc
//
LOCUS       DAZPEP010000281         3154 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZPEP010000281 DAZPEP010000000
VERSION     DAZPEP010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.283"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUT9_004344"
     CDS             <1..122
                     /locus_tag="ACHUT9_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1350652.1"
                     /translation="GTVNKSKVPLGLSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            456..2735
                     /gene="ydeP"
                     /locus_tag="ACHUT9_004345"
     CDS             456..2735
                     /gene="ydeP"
                     /locus_tag="ACHUT9_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFR1350653.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2983..>3154
                     /gene="safA"
                     /locus_tag="ACHUT9_004346"
     CDS             2983..>3154
                     /gene="safA"
                     /locus_tag="ACHUT9_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFR1350654.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTH"
BASE COUNT          864 a          713 c          746 g          831 t
ORIGIN      
        1 tggggacagt aaataaaagc aaagtacctc tgggtttaag cgcaacctat ggacaaacag
       61 gaaacaaggt ttcagccgga accgtccagt cagtcattgg tgtcactttt atctatgaat
      121 aattgtgatt ttgaccttag tctatgcgct ccattcacga aaattgcgca gataattatt
      181 ttgtgaaggc tattatccta cacctgtaag aaaatccgcg cacaattgaa gccagagtct
      241 tattttttat attgaaaaat atgttaattt aaacactatg agagatgatg cttaacttat
      301 cgtatttaat ctattgttta acgataatag attaaaaata gaaagagagt agtcccctac
      361 tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat cgctattaca
      421 aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt gaatcctacc
      481 agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta cgtaagcaga
      541 tggatatacg ccatgatgtt attgccatgt ttgacatgaa taagccagag ggctttgact
      601 gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt tgtgaaaacg
      661 gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct ttctttgctg
      721 agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct gcagggcgtc
      781 tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta agctggcaac
      841 aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat caggttgaat
      901 tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt tttgcccgtg
      961 aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg acaagcgtgg
     1021 gtttggcagc gagtatcggt gtaggtaaag ggaccgtgtt gctggaagac tttgagaagt
     1081 gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc atgctgactt
     1141 cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct ctacaggaac
     1201 gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg aactctgaga
     1261 ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg ttgctcaagg
     1321 ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt cggccctcat
     1381 tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta cgccgtgacg
     1441 ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag acacaaatcg
     1501 ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac ggaatgggga
     1561 tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg ctgttgatga
     1621 aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac tctaatgtac
     1681 agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg gctcgcctgg
     1741 gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt gccagcatgc
     1801 aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac tttgcactgg
     1861 caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatttg gcggtacacg
     1921 tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat attctgccgg
     1981 ttctggggcg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta accgttgagg
     2041 attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt gtaatgctga
     2101 aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccac agcgtggtag
     2161 cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa gctgtgttac
     2221 cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac ctgataaatg
     2281 cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt accagcaaag
     2341 ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg acagtacgta
     2401 gccacgatca gtacaacacg acgatttatg gtatggatga tcgctatcga ggggtattcg
     2461 gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg
     2521 gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg
     2581 atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc tattttccag
     2641 aatcgaatca catgctaact cttgataacc acgatccatt aagtggcatt cctggttata
     2701 aaagtattcc ggttgaatta gaaccatcaa attaattcgc ctcctcattt cttctgctgt
     2761 catccgcaca gcagaagaat ttctcattga ctattatcgc gcaatttgct cacattgatt
     2821 aaattaaacc acacactata agatataaac ctctgcctac agctgtaaga aactccgctc
     2881 agtactgaag tgccagtcct atttcctctt ttctccagcc tgttatatta agcatactga
     2941 ttaacgattt ttaacgttat ccgctaaata aacatatttg aaatgtatgc gaccacagtg
     3001 aaaaacaaaa tcacgcaaag agacaactat aaagaaatca tgtctgtaat tgtggttgtc
     3061 ttattactga cccttacgtt gatagccatt ttttcggcaa ttgatcagct gggtatttca
     3121 gaaatgggtc gcattgcaag agatcttaca catt
//
LOCUS       DAZPEP010000282         3136 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZPEP010000282 DAZPEP010000000
VERSION     DAZPEP010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.284"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            2..1197
                     /gene="bluF"
                     /locus_tag="ACHUT9_004347"
                     /pseudo
     CDS             2..1197
                     /gene="bluF"
                     /locus_tag="ACHUT9_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
     gene            1402..2133
                     /gene="bluR"
                     /locus_tag="ACHUT9_004348"
     CDS             1402..2133
                     /gene="bluR"
                     /locus_tag="ACHUT9_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFR1350655.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2354..2758
                     /locus_tag="ACHUT9_004349"
     CDS             2354..2758
                     /locus_tag="ACHUT9_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFR1350656.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2811..2921
                     /locus_tag="ACHUT9_004350"
     CDS             2811..2921
                     /locus_tag="ACHUT9_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350657.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHQANTIKLIFEM"
BASE COUNT          935 a          651 c          654 g          896 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgccg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtagca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattgaa tggtatccct tccatagcgt ggccagagaa aaaataattt
     1201 tcagcacatt ctttcacatg atttcagtaa atcatcgtga aaatgaatac gttatgttta
     1261 aatttaatcc acattaaata taatgtttca tccaatttag ctgaaatctt atttatactt
     1321 tgtacaattt tagcgtatat tgtacaaagt attattgggt cgtgtacagg cggcggagat
     1381 ttatgaccgc aaggaggaat tgtggcttat tacagcattg gtgatgttgc tgaacgttgc
     1441 gggattaatc ctgtcactct ccgggcctgg caacgccgct acggtttgtt aaaaccacag
     1501 cgcagtgaag gcggacaccg actctttgat gaagaagaca tacaacgcat cgaagagatc
     1561 aagcgttgga taagtaatgg cgtccctgta ggcaaagtta aagcattact ggaaaccacc
     1621 agccaggata cggaagatga ctggaaccgc ctgcaagaag agatgatgtc aattcttcgt
     1681 atggctaatc ctgccaaact acgcgcgaga attatttcac tgggtcgaga gtacccagta
     1741 gatcaattga ttaatcatgt ttaccttcct gttcgccagc gtctcgtgct tgatcacaac
     1801 acctcccgca ttatgagcag tatgtttgac ggcgcattaa ttgaatatgc agcagcctca
     1861 cttttcgaaa tgcgccgtaa gcccggtaaa gaagccattc tgatggcgtg gaatgttgaa
     1921 gagagagcac gattgtggct ggaagcatgg cgtttatcat tgtcaggatg gcacatttct
     1981 gttcttgctg atcccattga atcgccgcgc ccggaactgt tcccgacgca aacattgatt
     2041 gtctggacag gcatggcacc aacgagaagg caaaacgaac ttttacaaca ttggggtgag
     2101 caaggttata aagtcatctt tcatgccccc taatttcacc tttatcccgc cccttagaca
     2161 gaatgttata atctgaaccc acaacagcat ttgtaccgga gctggtccgt tgcaaaatgc
     2221 tcaccggaag aattcaggta atccctctat cacactctgt acatatcaca gccaaaggac
     2281 agaccagatt gaagtggtaa aaccgatcgt gcacactacg cagtctcaca actaacaaga
     2341 ggttattatc ctgatggatc aggtcgttgt ttttcaaaag atgtttgagc aagtgagaaa
     2401 agagcaaaac ttctcctggt tttattcaga attaaaacat caccgtattg cacattacat
     2461 ttattatctg gctacggata acatcagaat tattactcac gatgacacgg ttttgttatt
     2521 aagaggaacc aggaacctgt taaaagttag tacgaccaag aaccctgcta aaataaaaga
     2581 ggccgcattg cttcatattt acggaaaatc tacatttcgg gaatactgtt caacactggc
     2641 aggcgcgggc gttttccgtt gggttactga tgttaatcat aacaaacgca gttactacgc
     2701 cattgataat acgcttttat atattgaaga tgtagaaaat aataaaccat taatctagct
     2761 aaagttggat gcttaagaaa tgcttcataa ttcagtaagg cattagcata atgggaataa
     2821 aagtacagag actatcccta tggatgataa atactgtcct tttattgcta cccataaata
     2881 atcaccaggc caatactatc aaattgatat ttgaaatgtg atcacttgac tttctatacg
     2941 ttattttata acggttaata tatttataaa aacaacgggt gtgccatacg cccgtttcaa
     3001 tacttaacgc acatgtgttt tggtttagtc atcatccagt tatatgtatt ttagccagga
     3061 acaggttaaa cctttcctat ataactcaaa aattgaaacc ttattctcat gtcatgttta
     3121 tattcattat tatcgt
//
LOCUS       DAZPEP010000283         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZPEP010000283 DAZPEP010000000
VERSION     DAZPEP010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.285"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..424)
                     /locus_tag="ACHUT9_004351"
     CDS             complement(<1..424)
                     /locus_tag="ACHUT9_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1350658.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHR"
     gene            complement(430..1128)
                     /locus_tag="ACHUT9_004352"
     CDS             complement(430..1128)
                     /locus_tag="ACHUT9_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1350659.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(1128..1457)
                     /locus_tag="ACHUT9_004353"
     CDS             complement(1128..1457)
                     /locus_tag="ACHUT9_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350660.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPRWEATALESFLAEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            complement(1454..>3112)
                     /locus_tag="ACHUT9_004354"
     CDS             complement(1454..>3112)
                     /locus_tag="ACHUT9_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1350661.1"
                     /translation="ARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKKADDIWNLRKDD
                     YFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEASRLKYTEEAQK
                     AYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQSGVKV
                     SAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAENQFAVLEEAAQRR
                     QLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFAQQQRAKRASIE
                     AKSRGLTDRQAAREATEQRLKEEYGDNPLALNNVMSEQKKTWAAEDQLRGSWMAGLKS
                     GWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILL
                     KQAMVGIVGSIGSAIGGATGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIV
                     HRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASWTFEQNNHVVI
                     NNDGTNGQIGPQALKAVYDMGRKGARDEIQAQMRDGGLFSGGGR"
BASE COUNT          602 a         1038 c          750 g          722 t
ORIGIN      
        1 cgcgatgaaa atccggcatc tcaatccccg ccagatgata agcatcccgg aacagcgtgt
       61 aacagtccgt caccccgtgc tcaaagcgcc gcccggtgag atgcggcaca cagcggaact
      121 tatgaatcgt cccccggcag accagccacc acggcaaatc actctgcacc tgcagccgcc
      181 ggtcggcctc actcagccag ggcagaccac cggggtgact gtggaccagc gccacaatct
      241 caccctgcat ttctgcctgc agccagtctt ccggcgacat acggaaatac gcctccggct
      301 caccggagat attcacgcag gggaaatatc tttccccctc cggcgtgctt accacgaagc
      361 cgcacgactc cgctggcgca catcgccggg cgtgcgccag aatcgctgat tctgtctgtg
      421 tcatgggatt tactgcgaaa gtttgttaat ggaaaggaag ccgccaaagt tgccgacgtt
      481 attgcggaac ttacaaccgc tcaggcattt gctgcattta tccttcgtga tatcggacgt
      541 tggctggtca tattcatccg cgaccgccgg accgtgataa ccgcactcat caccgcgata
      601 ggtccaggtg caggtgttgg ccagcatgat acgtcccgga aaaacagcgc cgtccgtttc
      661 cgtcggcgtg gacagtacaa aagaggcact caccgcgctc agttcgctgc actgctcaat
      721 gcgccagcgg ctgatcacct cctgctccgg atcggcgtca ctgtttccgt tgacgaagtt
      781 caccgcatcc agaaaacggg cgtaaacctt acgccggacc accgttccgc cgaccagact
      841 ctgcagatct tccgccatcc cggtgaccat accgtacagg ttagaaaccg tcagcgtggg
      901 gcgcgtactg gtgcctttgc cattcagttc aaaaccactc ccctgaatgg gatacggctg
      961 atactgtcgc ccctgccagg tgaccggctc acctttttcg ttctgctcat tacagaaaaa
     1021 ataacgttct ccaccaacct ctgtcagatc gatttcccag agcaccacgc tggcagactg
     1081 ctccgcacgg gtgcattcat tcagtgtttc ctgccggata tcctgcatca gttcaccacc
     1141 tgttcaaact ctgcgctgaa ctcaacacgc agcatactga cccgcgacga ccattttgcg
     1201 caggtcacct ttatctgccg ccactcataa ggcggcgtcc acagaaagga tttccagccc
     1261 ccgtgctcag ccagaaacga ctccagcgcc gtggcctccc aacgggggac agaaagcgtc
     1321 acgctgtacg ttttcaggtt ggcattcagc ccggcaggcg ctcgctggga atagccatca
     1381 ccaaagcgca cctttcttac agaaggggcc gaagccacat ccatacctgg tttcactttc
     1441 cagcggaagg ttttcatcgt ccacctccgg agaacaggcc accatcacgc atctgtgcct
     1501 gaatttcatc acgggcaccc ttgcggccca tgtcatacac agccttcagc gcctgtggtc
     1561 caatctgccc gttcgtgccg tcgttgttaa tcaccacatg gttattctgc tcaaacgtcc
     1621 aggacgcctg cgaccggctg tctgccatgc tgcccggtgt accgacataa ccgccggtgg
     1681 catagccgcg catcagccgg taaagattcc ccacgccaat ccggctggtt gcctccttcg
     1741 tgaagacaaa ttcaccacgg tgaacaatcc ccgctggctc atatttgccg ccggttcccg
     1801 taaatccccc ggtcgcaaaa tggaatttcg ccgcagcggc ctgaatggct gtaccgcctg
     1861 acgctgatgc gccgccaccg gtagccccgc caatggcgct gccgatactc ccgacaatcc
     1921 ccaccattgc ctgcttaagc agaatttctg tcatcatgga cagcacggaa cgggtgaagc
     1981 tgcgccagtt ctgctcactg ccggtcagca tcgccgccat attctgcgca ataccatcaa
     2041 aggtctgcgt ggctgcactt ttaacctgcg acatactgtc cgtggcgctc tcttcccact
     2101 cactccagcc ggacttcagg cctgccatcc agctcccgcg aagctggtct tcagccgccc
     2161 aggtcttttt ctgctctgac atgacgttat tcagcgccag cggattatcg ccatactcct
     2221 ccttcaggcg ctgttccgtg gcttccctcg ctgcctgccg gtcagtcagc ccccggcttt
     2281 tcgcctcaat ggaggcccgt tttgcccgtt gctgctgtgc aaatttatcc gcctgctgcg
     2341 ccagcgcgtt caggtgctcc tgatacgtaa ccttgtcgcc aagtgcagcc agctggcgtt
     2401 tgtactccag cgtctcatct ttatgcgcca gcagggattt ctcctgtgca gacagctggc
     2461 gacgttgcgc cgcctcctcc agtactgcga actgattttc cgccttccac aaatcccggc
     2521 gctgctggct gattttctca ttcgctccgg catgcttctc cagcgtccgg agttctgcct
     2581 gaagcgtcag cagggcagca tgagcactgt cttcctgacg atcgcccgca gacaccttca
     2641 cgccggactg cttcggcttt ttcagcgtag cttcataatc ctttttcgct gccgccatca
     2701 gcgtgttgta atctgcctgc aggattttcc cgtctttcag tgccttattc agttcttcct
     2761 gacgggcggt atatttctcc agcggcgtct gcaggcgttc gtaagccttc tgcgcctctt
     2821 cggtatattt cagccgtgac gcttcggtat cgctctgctg ctgcgcattt ttgtcctgtt
     2881 gactctgctg ctcagccttc tttcgggcgg cttcaagcgc aagacgggcc ttttcacgat
     2941 catcccagta acgcgcccgc gcttcatcgt taacaaaata atcatccttg cgcagattcc
     3001 agatgtcgtc tgctttctta aacgcagcct ctgccttaat cagcatctcc tgagcggtat
     3061 caggacgacc aatatccagc accgcatccc acatggattt gaatgcccgt gc
//
LOCUS       DAZPEP010000284         3112 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZPEP010000284 DAZPEP010000000
VERSION     DAZPEP010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.286"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1659
                     /locus_tag="ACHUT9_004355"
     CDS             <1..1659
                     /locus_tag="ACHUT9_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1350662.1"
                     /translation="ARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKKADDIWNLRKDD
                     YFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEASRLKYTEEAQK
                     AYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPKQSGVKV
                     YAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQFAVLEEAAQRR
                     QLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFAQQQRAKRAAID
                     AKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQLRGSWMAGLKS
                     GWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTRSVLSMMTEILL
                     KQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTGTGGKYEPAGIV
                     HRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVI
                     QNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            1656..1985
                     /locus_tag="ACHUT9_004356"
     CDS             1656..1985
                     /locus_tag="ACHUT9_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350663.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            1985..2683
                     /locus_tag="ACHUT9_004357"
     CDS             1985..2683
                     /locus_tag="ACHUT9_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFR1350664.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            2689..>3112
                     /locus_tag="ACHUT9_004358"
     CDS             2689..>3112
                     /locus_tag="ACHUT9_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1350665.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHR"
BASE COUNT          743 a          738 c         1030 g          601 t
ORIGIN      
        1 gcacgtgcat tcaaatccat gtgggatgcg gtgctggata ttggtcgtcc tgataccgcg
       61 caggagatgc tgattaaggc agaggctgcg tttaagaaag cagacgacat ctggaatctg
      121 cgcaaggatg attatttcgt taacgatgaa gcacgggcgc gttactggga tgatcgtgaa
      181 aaaaaacgcc tggagcggga tgcagcccaa aagagggtag atcaacagcg tcaacaggac
      241 aaaaatgcgc agcagcagag cgataccgaa gcgtcacggc tgaaatatac cgaagaggcg
      301 cagaaggctt acgaacggct gcagacgccg ctggagaaat ataccgcccg tcaggaagaa
      361 ctgaacaagg cactgaaaga cggaaaaatc ctgcaggcag attacaacac gctgatggcg
      421 gcggcgaaaa aggattatga agcgacgctg aaaaagccga aacagtccgg cgtgaaggtg
      481 tatgcgggcg atcgtcagga agacagtgct catgcggccc tgctgacgct tcaggcagaa
      541 ctccggacgc tggagaagca tgccggagcg aatgagaaaa tcagccagca gcgccgggat
      601 ttgtggaagg cagaaagtca gttcgcggta ctggaggagg cggcacaacg tcgccagctg
      661 tccgcacagg agaaatccct gctggcgcat aaagacgaga cgctggagta caaacgccag
      721 ctggctgcac ttggtgacaa ggttacgtat caggagcacc tgaatgcgct ggcgcagcag
      781 gcggataaat tcgcacagca gcaacgggca aaacgggcag ccattgatgc gaaaaaccgg
      841 gggctgactg accggcaggc agcgcgggaa gccacggaac agcgcctgaa ggaacagtat
      901 ggcgataatc ctctggcgct gaataacgtc atgtcagagc agaaaaagac ctgggcggct
      961 gaagaccagc ttcgcgggag ctggatggca ggcctgaagt ccggctggag tgagtgggaa
     1021 gagagcgcca cggacagtat gtcacaggtt aaaagtgcag ccacgcagac ctttgatggt
     1081 attgcacaga atatggcggc gatgctgacc ggcagtgaac agaactggcg cagcttcacc
     1141 cgctccgtgc tgtccatgat gacagaaatt ctgcttaagc aggcaatggt ggggattgtc
     1201 gggagtatcg gcagcgccat tggcggggct gttggtggcg gcgcatccgc gtcaggtggt
     1261 acagccattc aggccgctgc ggcgaaattc cattttgcaa ccgggggatt tacgggaacc
     1321 ggcggcaaat atgagccagc ggggattgtt caccgtggtg aatttgtctt cacgaaggag
     1381 gcaaccagcc ggattggcgt ggggaatctc taccggctga tgcgcggcta tgcggaaggt
     1441 ggttatgtgg gcggtgccgg aagtccggcg cagatgcggc gggctgaagg cattaatttt
     1501 aatcagaaca atcacgtggt gattcagaac gacggtacga atggtctgcc aggtccacag
     1561 atgatgaagg ccgtgtatga catggcccgc aagggtgccc gtgatgaaat tcagacacag
     1621 atgcgtgatg gtggcctgtt ctccggaggt ggacgatgaa aaccttccgc tggaaagtga
     1681 aacccggtat ggatgtggct tcggcccctt ctgtaagaaa ggtgcgcttt ggtgatggct
     1741 attcccagcg agcgcctgcc gggctgaatg ccaacctgaa aacgtacagc gtgacgcttt
     1801 ctgtcccccg tgaggaggcc atggtactgg agtcgtttct ggaagagcac gggggctgga
     1861 aagcctttct gtggacgccg ccttatgagt ggcggcagat aaaggtgacc tgcgcaaaat
     1921 ggtcgtcgcg ggtcagtatg ctgcgtgttg agttcagcgc agagtttgaa caggtggtga
     1981 actgatgcag gatatccggc aggaaacact gaatgaatgc acccgtgcgg agcagtcggc
     2041 cagcgtggtg ctctgggaaa tcgatctgac agaggtcggt ggagaacgtt attttttctg
     2101 taatgagcag aacgaaaaag gtgagccggt cacctggcag gggcgacagt atcagccgta
     2161 tcccattcag gggagtggtt ttgaactgaa tggcaaaggc accagtacgc gccccacgct
     2221 gatggtttct aacctgtacg gtatggtcac cgggatggcg gaagatctac agagtctggt
     2281 cggcggaacg gtggtccggc gtaaggttta cgcccgtttt ctggatgcgg tgaacttcgt
     2341 caacggaaac agtgacgccg atccggagca ggaggtgatc agccgctggc gcattgagca
     2401 gtgcagcgaa ctgagcgcgg tgagtgcctc ttttgtactg tccacgccga cggaaacaga
     2461 tggcgctgtt tttccgggac gtatcatgct ggccaacacc tgcacctgga cctatcgcgg
     2521 cgatgagtgc ggttatcacg gtccggcggt cgcggatgaa tatgaccagc caacgtccga
     2581 catcacgaag gataaatgca gcaaatgcct gagtggctgt aagtttcgca ataacgtcgg
     2641 caactttggc ggcttccttt ccattaacaa actttcgcag taaatcccat gacagagaca
     2701 gaatcagcga ttctggcgca cgcccggcga tgtgctccag cggagtcgtg cggcttcgtg
     2761 gtaagaacgc cggaggggga aagatatttc ccctgcgtga atatctccgg tgagccggag
     2821 gcgtatttcc gtatgtcgcc ggaagactgg ctgcaggcag agatgcaggg tgagattgtg
     2881 gcactggtcc acagccaccc cggtggtctg ccctggctga gtgaggccga ccggcggctg
     2941 caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca caagttccgc
     3001 tgtgtgccac atcttaccgg gcggcgcttt gagcacgggg tgacggactg ttacacgctg
     3061 ttccgggatg cttaccatct ggcggggatt gagatgccgg attttcatcg tg
//
LOCUS       DAZPEP010000285         3046 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZPEP010000285 DAZPEP010000000
VERSION     DAZPEP010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.287"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..172
                     /locus_tag="ACHUT9_004359"
     CDS             <1..172
                     /locus_tag="ACHUT9_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350666.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="ACHUT9_004360"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="ACHUT9_004360"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFR1350667.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="ACHUT9_004361"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="ACHUT9_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3046
                     /locus_tag="ACHUT9_004362"
     CDS             2190..>3046
                     /locus_tag="ACHUT9_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFR1350668.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          710 c          836 g          805 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggatt
//
LOCUS       DAZPEP010000286         3005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZPEP010000286 DAZPEP010000000
VERSION     DAZPEP010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.288"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(10..165)
                     /locus_tag="ACHUT9_004363"
     CDS             complement(10..165)
                     /locus_tag="ACHUT9_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFR1350669.1"
                     /translation="MNTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(368..775)
                     /locus_tag="ACHUT9_004364"
     CDS             complement(368..775)
                     /locus_tag="ACHUT9_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1350670.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            852..1079
                     /locus_tag="ACHUT9_004365"
     CDS             852..1079
                     /locus_tag="ACHUT9_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFR1350671.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPWKVIHENQA"
     gene            1063..1484
                     /locus_tag="ACHUT9_004366"
                     /pseudo
     CDS             1063..1484
                     /locus_tag="ACHUT9_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
     gene            1562..2361
                     /locus_tag="ACHUT9_004367"
                     /pseudo
     CDS             1562..2361
                     /locus_tag="ACHUT9_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2368..>3005
                     /locus_tag="ACHUT9_004368"
     CDS             2368..>3005
                     /locus_tag="ACHUT9_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1350672.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKLGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVERRTASMRSVGMLTNLNYEVMKT"
BASE COUNT          792 a          649 c          829 g          735 t
ORIGIN      
        1 tcgttcattt tactcacctg aatgtcttcc caaccaacga cgtgcgccag cttcggtttt
       61 aaacgttttg cttttggtat acgtcatggc ggtgaatgtg ccgtcctggt tggggaacac
      121 gccgtacacc agagattcgt tgttgccaag atcgatagta ttcatgttga ccccatttcc
      181 ccttaacgcc ggggtagcgg aactgtttgc tgagaacacc gtgcggtgtc ttgatgaatg
      241 aaatttagaa taacctaagg tgggcggtca agatttttgt gtagaaaaac ctaagttttt
      301 tgatgtaaaa aacacaagtg tttgaaagtt tgtgcttttt attacagggt gtggagaaaa
      361 aaggggatta tttgtttgcg cttcttttgc gagctttgag tagttcttca aaaagtttgt
      421 tgaaattttc aactcgagca cgcatctctg acaacagggc cttttgctct gactcaggca
      481 gtgcgtcgaa cagttgaagt aactcttttt gatcttctgt cagaatggct ggctgattat
      541 ccgggatcgg ttcgcctggt tgtttatctt catccccaaa aagaagccaa gtcggtgagc
      601 actgaagcgc ttggctcagt gcgaataatc ttttccccgc cggctgtgtt tcatctcttt
      661 cccattgaga aattgttacg tgagccactt tgaccagctt acctaatgcg gcctgagaca
      721 gttttaattt tttacgccta tgtaagaggc gagcaccgaa ggtttcgttt ttcatattag
      781 ggaattctaa tttttcttga cttaggtttc tctacaatct agtttcctta ggaaaatcta
      841 aggagctcga tatgttgaaa attgatgcta tagcgttttt tggcagcaaa acaaagcttg
      901 ccaatgccgc aggagttagg ctggcaagca ttgctgcatg gggggaactg gttcctgaag
      961 gtcgcgcgat gcgcctgcaa gaggcatctg gcggggaact tcagtacgac cccaaagttt
     1021 atgacgaata tcgtaaggca aaacgacctt ggaaggtgat tcatgaaaat caggcatgag
     1081 cacatcgaat cagtgctgtt gccctggcag ccgaaaaagg gcaggcgtgg gtcgctaacg
     1141 caattactga agaatatctg cggcaggggg gcggcgaatt gtctctggta ccaggcaagg
     1201 actggaacaa tcagcagaat atctatcacc gttggttgaa aggtgaaacg aaagcgcaaa
     1261 gggaaaaaat tcagaaactg atccctgcgg ttctggcaat tcttccgcgt gagctgcgtc
     1321 accgactctg tatcttcgat accctggaac gccgtgcatt actggcggcg caggaagcgt
     1381 tgagtacggc aattgatgcg catgatgatg ctgtccaggc cgtttaccgg aaagcacatt
     1441 tcagcggtgg tgggtcgccc ggcgattctg tcgtagtgca ttgattgaaa ttaatcgtgc
     1501 cggattgttt tgttcggtat cagttaaatg taacgctgcg agcgttacaa ggtgaaaaca
     1561 aatggcttca aactggataa agctcgaggt tattacgccg gataagccgg aaatattcag
     1621 gcttgctgag attctgaata ttgatccaga tgccgcatta gggaaggtta ttcgcttctg
     1681 ggcatgggcg gatcaacaaa tgatagacgg taatgcagat tgtaacgctc gcggcgttac
     1741 aaaaagtgca atagatcgca tcacttttat gtctggtttt gctgatgcgt taattcaggt
     1801 tggatggctg gtcgaaaatg acggtgggct ttctctacct aactttgaac gtcataacgg
     1861 aaaaagctct aaaaaaaaac gggcggttac aaacgagcga gtaacaaaaa tacgcgaact
     1921 gaaacgaaaa ggtaacgctg ccagcgttac acaaacggat caaaaagcgt taccagagga
     1981 agaggaagag gaagaggaag aggaagagga agatctaaat actgatctcc ccctcgccaa
     2041 aaacgagcgt ctaaaaaatt cgagccggag gctattgagc tgcccgattg gttgccggaa
     2101 acactctggc atgagtgggt ccggttcaga caggcattgc gaaaaccgat tcgaacggag
     2161 cagggcgcta acggggcgat acgggaactg gaaaaattcc gtcagcaggg ttttacacct
     2221 gagcaggtga ttcgacacag catcgccaat gaataccagg gcctgttcgc gccgaaaggt
     2281 gttcggcctg agacgttgct ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc
     2341 ccgccaggct tcagggggta acggaccatg aaaaatattg cgacaggcgg cgttctggaa
     2401 cgcatccgcc gactgacccc gccacatgta accgccccat tcagaacggt agcggagtgg
     2461 cgcgagtggc aacttgctga aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc
     2521 cagttgcggg tggaaaaaat tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc
     2581 tcgtttgcga attaccaggt gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa
     2641 tccatcgccg atgaactgat gaccgggtgt acaaattttg cgttcagcgg aaaacttggt
     2701 accgggaaga atcacttagc ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca
     2761 gtgattgtgg ttaccgtggc tgatgttatg agcgccctgc acgccagcta tgacgacggg
     2821 cagtcaggcg aaaaattttt gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa
     2881 attggcattc agcgcgagac gaaaaacgag caggtggtgc tgcaccagat tgttgagcgc
     2941 cggacagcgt cgatgcgcag cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa
     3001 acatt
//
LOCUS       DAZPEP010000287         2992 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZPEP010000287 DAZPEP010000000
VERSION     DAZPEP010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.289"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUT9_004369"
     CDS             <1..1076
                     /gene="bglB"
                     /locus_tag="ACHUT9_004369"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFR1350673.1"
                     /translation="RLFDEMAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEH
                     YARTVFTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARA
                     VKACHSLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYM
                     QRFFRDHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPN
                     PHLKSSEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIA
                     YLNDHLVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLT
                     RTRKKSFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUT9_004370"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="ACHUT9_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFR1350674.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="ACHUT9_004371"
     CDS             2805..>2992
                     /locus_tag="ACHUT9_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HFR1350675.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAZPEP010000288         2938 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZPEP010000288 DAZPEP010000000
VERSION     DAZPEP010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2938
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.290"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            37..264
                     /locus_tag="ACHUT9_004372"
     CDS             37..264
                     /locus_tag="ACHUT9_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFR1350676.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="ACHUT9_004373"
     CDS             complement(265..639)
                     /locus_tag="ACHUT9_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFR1350677.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUT9_004374"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="ACHUT9_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2099..2674)
                     /locus_tag="ACHUT9_004375"
     CDS             complement(2099..2674)
                     /locus_tag="ACHUT9_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFR1350678.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLINHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          761 a          694 c          689 g          794 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt actgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataaagattc
     1921 ttgtgcagtc tgaatagata acttagcgtt atgtctagtc gtttccctct ccattcctaa
     1981 aaagtgctgt atactggcct atacaacgct ataaagacct ctacatagcg ccttgatagc
     2041 tatgtcacag gtacaaaaaa gccccctata aaggaggctg tgtagtctat taagggtctt
     2101 acagggtctt ggtccagtgt ctgctaacag tccttacaga gaggttaaga accttagcta
     2161 cctgagactg agatagtccc ctttccttgt actctaagac cgctctagtt gtgtcactcg
     2221 ctataggtct tccaagtacc ttaccttgaa ctctagcacg ctctagaccc tgtttagtgc
     2281 gctctctgat acggtttctt tcaaactctg caaaggtact aaatagctgt agaaccaact
     2341 taccctctga gcttgaaagg tctttaacgg gtaaatctaa acagatgagc ttcacaccct
     2401 tatcattaag aagggtcact gtattctgta ggtcgatgtt atcccttccc agacggtcaa
     2461 gtttaagaac aacaagggtg tcaccttctt ctagcttatg gttgatcaga ttcgcaaatc
     2521 cgtccctatc catagctgac actgaaccag agacaacctc ctctataact cgtgacttag
     2581 gaatatcata gccagccttc ttaatggctt gaatctggtt ctctgtggtg gaagtctcta
     2641 ctgatacacg gcagtaagca aaggttcgtg acatagatat ccctctgggt agtcattaaa
     2701 cggttagttt gtcttgtcta tagacattaa agcaaatggt aggtttgttg tctatagcta
     2761 tagtgactct tctatgtaga tattaaacgg acgtttcttg tcgtgggtca catagatact
     2821 tttagggctt ctgtcaagtg atattcgtag attttgcaag ggggaggtgt aaggctgaaa
     2881 cagggctact aagaacacat acatctaagg ctggaaagta cctgagcaga tattcttc
//
LOCUS       DAZPEP010000289         2900 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZPEP010000289 DAZPEP010000000
VERSION     DAZPEP010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.291"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1199)
                     /locus_tag="ACHUT9_004376"
     CDS             complement(<1..1199)
                     /locus_tag="ACHUT9_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFR1350679.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="ACHUT9_004377"
     tRNA            1492..1586
                     /locus_tag="ACHUT9_004377"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1858..2900
                     /locus_tag="ACHUT9_004378"
                     /pseudo
     CDS             1858..2900
                     /locus_tag="ACHUT9_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          845 a          691 c          665 g          699 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa
//
LOCUS       DAZPEP010000290         2878 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZPEP010000290 DAZPEP010000000
VERSION     DAZPEP010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.292"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHUT9_004379"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="ACHUT9_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFR1350680.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="ACHUT9_004380"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="ACHUT9_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFR1350681.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="ACHUT9_004381"
     CDS             1650..2099
                     /locus_tag="ACHUT9_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFR1350682.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHUT9_004382"
     CDS             complement(2100..2333)
                     /gene="ydcY"
                     /locus_tag="ACHUT9_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFR1350683.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHUT9_004383"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="ACHUT9_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFR1350684.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHUT9_004384"
     CDS             2787..>2878
                     /gene="yncL"
                     /locus_tag="ACHUT9_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFR1350685.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          757 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgccag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggtc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttat
//
LOCUS       DAZPEP010000291         2875 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZPEP010000291 DAZPEP010000000
VERSION     DAZPEP010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.293"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            8..511
                     /locus_tag="ACHUT9_004385"
     CDS             8..511
                     /locus_tag="ACHUT9_004385"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFR1350686.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(557..973)
                     /gene="rraB"
                     /locus_tag="ACHUT9_004386"
     CDS             complement(557..973)
                     /gene="rraB"
                     /locus_tag="ACHUT9_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFR1350687.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1135..2139
                     /gene="argF"
                     /locus_tag="ACHUT9_004387"
     CDS             1135..2139
                     /gene="argF"
                     /locus_tag="ACHUT9_004387"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFR1350688.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2211..>2875)
                     /locus_tag="ACHUT9_004388"
     CDS             complement(2211..>2875)
                     /locus_tag="ACHUT9_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1350689.1"
                     /translation="MQYFGDSEKIKYACSVINHIDFSCDQSKDFSCDQSKIKIAEALF
                     FNLDKEPYKNSRKLQELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSI
                     LVNDIFLKDFFMKQPDFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPG
                     YVVKIFEEKMGNELKIRTRMMKILRNGKINIYGYINEEQLGKLNPPEDLRIAIKKLGW
                     NN"
BASE COUNT          780 a          667 c          626 g          802 t
ORIGIN      
        1 ttaaactatg aataacattg cgccgcaatc acctgtaatg cgtcgcctga cgctgctgga
       61 taatcctgct atcgcccgcg tcattcgtca ggtatccgcc gaatacggtc ttaccgctga
      121 taaaggctac accgtcgccg atccgaatct tgacgagctg tatcaagtgt atagtcagcc
      181 tggccatgca tattgggtgg ttgagtacga gggtgaagtg gtcggcggcg gtgggattgc
      241 gccattaacc gggagtgagt cggacatttg cgaactgcaa aagatgtatt ttcttccggc
      301 tattcgcggt aaagggctag cgaaaaaact ggccttaatg gcgatggagc aggcacgaga
      361 gatgggcttc aaacgctgct atctggaaac gaccgctttt ttaaaggaag ccattgcgct
      421 ttatgagcat ttgggctttg agcatatcga ctatgcgctt ggctgcacgg gccatgtcga
      481 ttgtgaagtg cggatgctac gcgaactcta aaaaaatgcc cgctagtatg tgctatcggg
      541 cattttaatc aattaattag tggcgaaccc cgtcatcgtc ttcatcgaca aaatcttcat
      601 cgtcgccatc ttcgccgtta ggatcttcaa agtaagtgcc ccaaccgtcg tactcaacgt
      661 caaatttctc cgccagcgtc atcagttgtt caacctgggc atcgatcaga tcggcattca
      721 acgcgcactc gctgaggatg tcgcagcaaa tcacgatatc accatcttca acttccagct
      781 cttctggatc ggtcacttcg taaccgagtt taaacgcttc aactgccgct ttttccagag
      841 tttccagatc gtctgcggaa agatggtgtt cgatggtgta caacgcgtcc ggatcgctgc
      901 catcttccag taattcttca ataatcaaac gtgtttcttc acgctgttct tccagttgtt
      961 ccgggtttgc catggctcat tcctcttaat gtcctgccga tactcttatt gtcacacacc
     1021 actgacattg cctccacctt tgtgataaag atttatgctt tagacttgca aatgaataat
     1081 catccatata aattgaattt taattcatta aggcgttagc cacaggaggg acctatgtcc
     1141 gggttttatc ataagcattt cctgaaatta ctcgatttca cgccagctga actcaacagc
     1201 ctgctgcagt tagccgcgaa gctgaaagcc gataagaaaa gcggtaaaga agaagccaaa
     1261 ctcactggta aaaacatcgc gctcatcttc gaaaaagact cgacccgtac ccgatgctct
     1321 ttcgaagttg ccgcatatga ccagggggct cgtgttactt atctcggccc aagcggcagc
     1381 caaattggtc ataaagagtc gattaaagac actgcccgcg tgcttggtcg catgtatgac
     1441 ggtattcagt atcgcggcta tggtcaggag attgtcgaaa cactggcgga atacgctggc
     1501 gtgccggtat ggaacggcct gaccaatgag tttcacccca cgcagctgct ggcggatctc
     1561 ctcaccatgc aggagcattt gcccggcaaa acgttcaacg aaatgacgct agtctatgca
     1621 ggtgacgcac gtaacaacat gggcaattcg atgctcgaag ctgcagcgct taccggtctg
     1681 gatttgcgtc tggtcgcgcc acaggcatgc tggccggaag ctgcgctggt tacggaatgc
     1741 cgcgccctgg cacaacaaaa tggcgggaat attacgctga cggaagatgt agctaaagga
     1801 gttgaaggtg ctgactttat ctataccgat gtatgggtgt cgatgggtga agcaaaagag
     1861 aaatgggctg aacgcattgc attgctgcgt gattatcaag tgaacagcaa gatgatgcag
     1921 ttgaccggta acccggaggt caaattcctc cactgcctgc ccgcgtttca tgacgaccaa
     1981 acgacgcttg gcaaaaaaat ggcggaagag tttggcctac atggcggaat ggaagtgact
     2041 gatgaggtct tcgaatctgc cgccagcatt gtatttgatc aggcggaaaa ccgcatgcat
     2101 accatcaaag cggtgatggt cgcgacgctc agtaaataat tgatatcgct ataaagatgc
     2161 cgggaaatac aaaaacatcc cggcgtcaca ttattttatg cagtgatagt tcaattattc
     2221 caccccaatt tttttattgc tatacgtaaa tcttccggag gatttaattt tcctaactgt
     2281 tcttcattaa tatatccata tatattgatt ttgccattcc ggaggatttt catcattctt
     2341 gttctaattt ttaattcatt acccatcttt tcttcaaaaa tcttaacgac ataacccgga
     2401 agtttattta caatttcttt tgttttttct tcatcaccat aaacatatcc taactcatta
     2461 agatacagct gttcgccctc ataacactta agtaattgat agaagtacca cttagcaaaa
     2521 tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat ttactaaaat gctgacaggc
     2581 acttcattaa atagtaactt aacatcatca aataacctta gtattaagcg tgatttttct
     2641 tgattgctta agctaaaatc attgacatat gcaaccaact tatcccaaat caattcctgt
     2701 aatttacggc tatttttata gggttcttta tcgagattga aaaataaggc ctcagctatt
     2761 ttaattttag attgatcgca gctaaaatct ttagattgat cgcagctaaa atcaatatga
     2821 tttatcacgg agcatgcata ctttatcttt tcagaatcac caaaatattg cattc
//
LOCUS       DAZPEP010000292         2835 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZPEP010000292 DAZPEP010000000
VERSION     DAZPEP010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.294"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            188..772
                     /locus_tag="ACHUT9_004389"
     CDS             188..772
                     /locus_tag="ACHUT9_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1350690.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            877..1617
                     /locus_tag="ACHUT9_004390"
     CDS             877..1617
                     /locus_tag="ACHUT9_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFR1350691.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1652..2835
                     /gene="htrE"
                     /locus_tag="ACHUT9_004391"
                     /pseudo
     CDS             1652..2835
                     /gene="htrE"
                     /locus_tag="ACHUT9_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          817 a          591 c          605 g          822 t
ORIGIN      
        1 aagaaaattt aatttgttca aaagccctta aagtgcacaa taggcgtcct aatgaacaat
       61 ggttgtaaat tttggtaaca aatatcattg ctcattaata tctagcatct gcttataaca
      121 tttgtgtcat gaatagtcat tcttcaaacc agtagtctga ctaatggaat tttattcaag
      181 gatttttatg tctaaaaaat taggttttgc cctgtctggt cttatgctgg caatggttgc
      241 aggtactgct tctgctgata tggatggtgg tcagttaaat atcagtggtc tggttgttga
      301 taacacctgt gaaacccgcg ttgacggcgg taataaagat ggtctgatcc tgctgcaaac
      361 tgcaactgtt ggtgaaatcg atgctggcgt gctgaatgac accgttggcg ctaaagccaa
      421 acctttcagc atcaccgttg actgcagcaa agcaaatccg aatccaggta gtacagctaa
      481 aatgaccttc ggttctgttt tctttggtaa tagcaaagga acgctgaaca acgacatgtc
      541 tattaacaac ccgtctgacg gcgtgaatat cgctctgcac aatattgatg gttccactat
      601 caaacaggtt caaattaaca accctggcga tgtgtatact aaagccctgg atgcaacgac
      661 aaaatctgct gtttatgatt ttaaagcgtc ttacgttcgt gccgtcgcag accaaacagc
      721 aactgctggt tatgtaaaaa ctaacactgc atacaccatt acttatcagt aagtattcac
      781 caattaaatt gattgaatga atatacaggg aatagtgtta ataatttcta tttttatatt
      841 attccctgtt ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca
      901 acagctttat gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggacattgtc
      961 atttcgggta ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa
     1021 aataaaggga ataacccgtt gcttgtccag agttggttag atactggcga taacaacgct
     1081 gagcctggca gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc
     1141 aaacgtgggc aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga
     1201 gagagcgtgt tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc
     1261 gcgaatcaaa gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg
     1321 gatggattga agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt
     1381 tcagaaggta aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc
     1441 agtggtgatt tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca
     1501 tttagtgatg aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta
     1561 catttttacg ccattaatga ttttggtggc gccattgaag gtaatgccag tctgtaatca
     1621 ggcaaggata taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca
     1681 taatcatctg acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg
     1741 tgctgaactt gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga
     1801 tctcagccgg tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta
     1861 tgtcaacgat caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa
     1921 aacgcacaag cctgtatcac acttaagaat ttattgcagt ttcatataaa ttctcccgat
     1981 ataaataacg aaaaagccgt tctgcttgcc agggatgaaa cactcggcaa ttgccttaat
     2041 ttgacggaaa ttatccctca ggcttctgtt cgttatgacg ttaacgagca acgtctggat
     2101 atagacgttc ctcaggcatg ggtaatgaaa aattaccaaa actatgttga tccatcgtta
     2161 tgggaaaatg gcattaatgc ggccatgttg tcatacaacc tcaacggata tcatagtgaa
     2221 actcctggtc gaagaaatga cagcatttat gctgctttta acggtgggat gaatttaggt
     2281 gcatggcgac tgcgtgcctc aggcaactac aactggatga ccgattctgg cagtaattat
     2341 gattttaaga atcggtatat acagcgtgat atcgcctcgc tgcgttctca actcattctt
     2401 ggtgagtctt atacaacggg cgaaaccttt gattctgtca gtatccgcgg cattcgttta
     2461 tacagcgaca gccgcatgtt gcctccgaca ttagcgagtt ttgcgcctat cattcatggc
     2521 gttgctaata ccaacgccaa agtcactatt acgcaaggtg gctataagat ttatgaaacg
     2581 actgtgccgc caggcgcttt cgtcattgat gatctgagtc cgtcaggtta cggcagcgat
     2641 cttattgtta ctgtcgaaga atccgatggc tcaaagcgga cattctcgca acctttttca
     2701 tcggtcgttc aaatgttacg ccctggcgtt ggacgttggg atattagcgg cggccaggtc
     2761 ttaaaagatg atattcagga tgagcctaat ttatttcagg ccagctacta ctacggtctg
     2821 aataactatc taacg
//
LOCUS       DAZPEP010000293         2729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZPEP010000293 DAZPEP010000000
VERSION     DAZPEP010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.295"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(423..680)
                     /locus_tag="ACHUT9_004392"
     CDS             complement(423..680)
                     /locus_tag="ACHUT9_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350692.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="ACHUT9_004393"
     CDS             complement(677..1174)
                     /locus_tag="ACHUT9_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFR1350693.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="ACHUT9_004394"
     CDS             complement(1376..1834)
                     /locus_tag="ACHUT9_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1350694.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="ACHUT9_004395"
     CDS             complement(1831..2328)
                     /locus_tag="ACHUT9_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFR1350695.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUT9_004396"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="ACHUT9_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFR1350696.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          649 a          708 c          621 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatgtct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataaccaa
//
LOCUS       DAZPEP010000294         2643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZPEP010000294 DAZPEP010000000
VERSION     DAZPEP010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.296"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..656
                     /locus_tag="ACHUT9_004397"
     CDS             <1..656
                     /locus_tag="ACHUT9_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1350697.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLAIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSHS"
     gene            614..1291
                     /gene="bglJ"
                     /locus_tag="ACHUT9_004398"
     CDS             614..1291
                     /gene="bglJ"
                     /locus_tag="ACHUT9_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFR1350698.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHUT9_004399"
     CDS             complement(1329..2117)
                     /gene="fhuF"
                     /locus_tag="ACHUT9_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFR1350699.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2258..2494
                     /locus_tag="ACHUT9_004400"
     CDS             2258..2494
                     /locus_tag="ACHUT9_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFR1350700.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2533..2619)
                     /locus_tag="ACHUT9_004401"
     tRNA            complement(2533..2619)
                     /locus_tag="ACHUT9_004401"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2583..2585),aa:Leu,seq:cag)
BASE COUNT          681 a          592 c          671 g          699 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc aattcacagc aacgctgaaa
      541 tgtatgcatg gataaatagc gcgcagggtg caagagaact taacttgcct tctgtttatg
      601 gagatgccgc agaatggaac acagccgaat taagaagaga aatgtcgcac tcatagaaaa
      661 atgcgtcatg agcagtatcg gtattgagag tttattcaga aagtttgcgg gtaaccctta
      721 taagctccat acctatacca gtcaggagtc atttcaggat gccatgtcgc ggatctcatt
      781 tgcggcggtc attttttctt tttctgccat gagaagtgag cgcagagagg gattatcttg
      841 cctgactgaa ctggcgatta agtttccgcg tacccggcgt ttagttattg cggatgatga
      901 tattgaagct cggctgattg gttcattgtc gccatcaccg ctggacggtg tattaagtaa
      961 agcgtcaacg ctggagattt ttcatcagga actctttttg tcattaaatg gtgtacgtca
     1021 ggcgaccgac cgactgaaca atcagtggta cattaaccaa agccggacgc taagcccgac
     1081 ggagagagaa atattgcgct ttatgtcgcg tggctactca atgacacaaa ttgccgagca
     1141 gcttaaacgc aatatcaaaa cgatccgtgc acataaattt aatgtgatgt cgaaactggg
     1201 cgtcagttct gatgcagggt tgttggaggc cgcagatatt ctgttatgta tgcggcattg
     1261 cgaagcaagt aatgtgttgc atccctatta atccgcatga tgccgggttt acttccccgg
     1321 cagtgctttc atttcagcgt acaatcgcca cattgctgca catccggtaa gcgataacgc
     1381 tggcagcagg ttcggcgcac cagcaggcca tcgcgcagca cgacggtacg ccagagtgga
     1441 ttatcttcac cgttcgtgag cgttttctca aaaaagaggg catggcgcag cgattcaacc
     1501 gtcgcctcgc cgagcagttg cttcatctca gtgagatacc agttgatcaa ataaccggta
     1561 ttactccaga taagtttgcc gttgatctct ccggtcgctt ctagtgcctg cacaaccgga
     1621 accagcgcct ggctgattaa cgtttccatt cgctgctgcg gcgaatgtgg tgttgcgttt
     1681 ttatcttcac acacatcgac ccagaaacag gcgacgcgtc cggtttcgtg aaactcagca
     1741 tggaaatgtt ccggcgacac atctaatgcc ttttcctgcg tcagtagcgc cagcattaat
     1801 ggtggcacca tcaggccgat atacaattgt gcccatagtg agatcagcgg tttgttctcg
     1861 cggatcatcg ttggttggtt gcgatagata tgatcggaat agaccgcgag tagagaactt
     1921 agcgcattcg gtgatgacca ttgcgccagc gtcatggcgt taagcggagc gggttcatcc
     1981 aggcggataa actcgagcaa atgttcacga tgtttggcga tcgtcgcacg tacggcttgt
     2041 gcaagcccag catcctgcgg ctggagatgc gttcgccaga tgatatcttc atagagcggt
     2101 gcggaacgat aggccataat cgggatagta atctaaatga taatgattgc taatcctagc
     2161 gataggttta cccgatagca agggatttct ctggcttgca aatgataaaa attatcatat
     2221 gatattggtt atcattatca atgaaagaga tgaaaccatg ttgcaacgta cgctgggcag
     2281 tggctgggga gtgttgctgc cgggattgct gattgcaggg ctgatgtatg cggatttatc
     2341 gccggatcag tggcggattg tcattctgat gggattagta ttgacgccgg tgatgctgta
     2401 tcacaaacag ttacggcatt acgttttgct accatcgtgc ctggcgctta ttgctgccat
     2461 catgctgatg ataatgaatt tgaatcaggg atgaaaaatc aaggaagaaa caagaaagga
     2521 agtaaagata attggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     2581 acctgaagct agcgcgtcta ccaattccgc caccttcgca cagtcatctt actttttttg
     2641 ata
//
LOCUS       DAZPEP010000295         2587 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZPEP010000295 DAZPEP010000000
VERSION     DAZPEP010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.297"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            196..1149
                     /locus_tag="ACHUT9_004402"
     CDS             196..1149
                     /locus_tag="ACHUT9_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFR1350701.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHUT9_004403"
     CDS             complement(1190..2185)
                     /gene="pgl"
                     /locus_tag="ACHUT9_004403"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1350702.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2481..2576)
                     /locus_tag="ACHUT9_004404"
                     /pseudo
     CDS             complement(2481..2576)
                     /locus_tag="ACHUT9_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
BASE COUNT          714 a          637 c          578 g          658 t
ORIGIN      
        1 tatttttttc ataataatgc cctcaattta aaaacataac ttattgcgat atggttacat
       61 taagggcaaa gcatctctaa tgactttagc tcaacgatta ataacatttt ttcattaatt
      121 taagttggct aaatgcagtt aaaattttta acggccagcc acccaaaatc gtcgcttcca
      181 ttttccggag taataatgaa gcatgaatta tcaagtatga aggcatttgt catactggca
      241 gagtccagtt cgtttaacaa tgccgctaaa ttactcaata ttacgcaacc tgcattaacg
      301 cgaagaataa aaaagatgga ggaagattta catattcaac tatttgagcg tacaactcgc
      361 aaggttacgt taacaaaagc aggaaaaagg ttgctcccgg aggcgcggga attaataaag
      421 aaattcgatg aaacgctttt taatattcgt gatatgaatg cttatcatcg tggtatggtg
      481 acgttagcgt gtattccgac cgcagtattt tattttttac cgctggcaat cggtaaattt
      541 aatgagctgt atcccaatat taaagtgcgg attctggaac aaggcacgaa taattgcatg
      601 gaatcggtgc tgtgtaacga atcagacttt ggcataaaca tgaacaacgt cacaaattca
      661 tccattgatt ttaccccact ggtcaacgag ccgttcgtgc tggcctgtcg gcgtgaccat
      721 ccattggcca aaaagcagct tgtagaatgg caggagttag tgggttacaa aatgattggc
      781 gtacgctcct ccagcggcaa ccgactgttg atagaacaac agctggccga caagccctgg
      841 aagctggact ggttttacga agtgcgtcat ctttcgacgt cattaggact ggtagaggcg
      901 ggactgggga tttcggcgct tcctggtctg gcaatgcccc atgcgcccta ttcttcgatt
      961 attggtattc cactggtgga acccgttata cgtcggacat tggggattat tcgccgcaaa
     1021 gatgccgtgc tttctccggc agcagaacgc ttttttgcct tgctgattaa tttgtggact
     1081 gacgataaag acaatttgtg gaccaatatc gtcgaacgcc agagacatgc gttacaggag
     1141 ataggttaat acaccggcgc aggagagact gcgccgggta aatcagcggt tagtgtgcgt
     1201 taaccaccac ccacattggt ccctgcccga ccgcatagcg gcctttttca tgcagtagcc
     1261 cctgctcgcc aacaatttcg tataccgaga tgtggtgaga tttttgcccg gcggcaatca
     1321 gatacttgcc gctgtgatca acattgaagc cgcgcggctg ggtttccgtt ggctggaagc
     1381 cttctttact caacacgctg ccatcttccg aaacgctgaa aacggtaatc agactggcgg
     1441 tacggtcgca ggcgtataaa tgacgaccat ccggggtgat atgaatatca gccgcccaac
     1501 aggtgtcgga gaagttttcc ggcatcatat ccagcgtctg gacacattca atattgccgt
     1561 gtggatcttt cagttcccag acatccactg agctgtttaa ctcattgacg caatacgcat
     1621 attgttcgtt tggatggaat accatatgac gcgggccggc cccttcaacg gtagtcactt
     1681 ccgcagggtc ctgcgccacc agatgaccat catcgctgac cgtaaacagg caaatgcgat
     1741 cctgctttaa tgccggaacc cacagcgtac ggttgtccgg tgagatattg gcggaatggc
     1801 aaccgtccag cccctcgacc acatcgacga cgcccactgg caggccatct tccagacgcg
     1861 ttacgctcac gttacccgca ttgtaagaac ctacaaagac aaactgcccc tggtgatcgg
     1921 tggaaatatg cgtcggacta cccggcagcg cagactctgc ggcaaaggtc agtgcgccat
     1981 cgtccggggc aatacgatac gccaggacgc gaaactcagg gcgaacacca acatagagat
     2041 aacgtttgtc cgggctgacc accatcggct gcacctgccc cggcacatcg acaacctgtg
     2101 tcagcgtcag tgcgccttca tgattcaggt tccagacgtg aatttgctgg ctctcagggc
     2161 tggcgatata aactgtttgc ttcatgaaca cacctcaaaa atgataaatc ccttatcttt
     2221 cacacacata acaacactta ctggataaaa aacagacaac aaaacacgat aaaatacaat
     2281 atcgcacatc aattaaatca ataagttagc actccatttc gaatggcata ttactaaaat
     2341 attagtcacc gacacgaatt tgacaatgcc acagatattt tcgcgactat tcagggagta
     2401 tgactctccc cgcttttgtt tacctgtatc aacgaccgta aacactatgc cataacaact
     2461 tctggccaac ttcccattcg ctcaacaatc aactcacttc cggcacaaat aaacgcatta
     2521 cgttgtagat cggtattctg gtcatttgtt gatacgcgta catagccaat aagcatggta
     2581 aatcccc
//
LOCUS       DAZPEP010000296         2557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZPEP010000296 DAZPEP010000000
VERSION     DAZPEP010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.298"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUT9_004405"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="ACHUT9_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFR1350703.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="ACHUT9_004406"
     CDS             complement(390..1178)
                     /locus_tag="ACHUT9_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFR1350704.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSWIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUT9_004407"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="ACHUT9_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFR1350705.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUT9_004408"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="ACHUT9_004408"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFR1350706.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2557)
                     /gene="pagP"
                     /locus_tag="ACHUT9_004409"
     CDS             complement(2092..>2557)
                     /gene="pagP"
                     /locus_tag="ACHUT9_004409"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFR1350707.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          653 a          659 c          580 g          665 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cagctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgg attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgtt
//
LOCUS       DAZPEP010000297         2548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZPEP010000297 DAZPEP010000000
VERSION     DAZPEP010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.299"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..82
                     /locus_tag="ACHUT9_004410"
                     /pseudo
     CDS             1..82
                     /locus_tag="ACHUT9_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415662.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            829..1674
                     /locus_tag="ACHUT9_004411"
     CDS             829..1674
                     /locus_tag="ACHUT9_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1350708.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGIATPKSFTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQSQDWPGSVMTGCSNPVRLTTHEIATSGGELIKFIAEAAIMATT
                     LSHPCVTVENGRAVTTSVAVAEFFHKRHDNVLRAIANIECSDKFTALNFEASEYTDSI
                     GRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPADKMIPG
                     DGRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQL
                     LKIHC"
     gene            1685..1873
                     /gene="dicB"
                     /locus_tag="ACHUT9_004412"
                     /gene_synonym="ftsT"
     CDS             1685..1873
                     /gene="dicB"
                     /locus_tag="ACHUT9_004412"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR1350709.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            1861..2061
                     /locus_tag="ACHUT9_004413"
     CDS             1861..2061
                     /locus_tag="ACHUT9_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR1350710.1"
                     /translation="MCTMNTVFALVLTVFLVSGEPVDMVTGVYSSMKECMTAAAKQKI
                     PGDCYPVDKVIHHDNNEIPAGL"
     gene            2146..2421
                     /locus_tag="ACHUT9_004414"
     CDS             2146..2421
                     /locus_tag="ACHUT9_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350711.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          745 a          557 c          599 g          647 t
ORIGIN      
        1 agatgcaaaa cgcctccagc aactggagcc aaatgcaggc actggggcac gcatctggct
       61 ggctaaagaa gcgctggaat ctggcgatta tgatagcgaa gaagccttct acaaagcaga
      121 aggccgtaca ggatattcac cgggtcttgg cggggtataa ataccatgcg cattgactga
      181 attcgcaaac aaaaacagac gcgcgatacc tggatagtcg gtctgcatag tcagatatct
      241 ggctgcgaat ttgataaata tcaccgccct tttcggaaac ataagttccg tccgggagtt
      301 gattatacga accatctcca tgggggatcg ttctcctgaa tcctagtgag agcatatatt
      361 tatgaagccc ttcgtaatcc tctggctcag cattatatag ttctactctg gcgagatacg
      421 ttggcatatt catttcctta ctggttgtgt gagaacttca gtaaagatac caccaaagcc
      481 cggaggtggt gaaataaaac cgggcacaac acgaaggcgc atttccggta tccataaaga
      541 gtcggtcttg tctgttaaat ttaaatggtg ggagtgcgcc tccggttgta aataacgaca
      601 ttgctgtgtg aagtaccagt tggcggcatc ggtttaattg ctggctgatg tccgcccttt
      661 ttaaagtgaa ttttgtgatg cggtgaatgc ggctcagcgc acgcggaaca gttaaaacag
      721 taaggaaaat cccttattcc ggcgttaatt gttaactggt taacgtcacc tggaggcacc
      781 aggcaccgca tcaacaaagt tcacttcggt gatgaaagga aagagaaaat gttgaatgta
      841 gctattgaaa accagaacgg gtggaattat agtgcacctg cacctcataa aacgggtgcc
      901 gggattgcta ccccgaagtc attcacggcg cataaccgcg ctcaggcggt ttttttatgc
      961 gtaatgcaca gccacattca aattatggtg gggcgtgcgg ggcagtcgca agactggccg
     1021 ggttccgtga tgaccggttg tagcaaccct gtacgtctca ccacccatga gattgctacc
     1081 tccggtggtg agttaatcaa attcatcgcg gaggctgcca tcatggctac taccctttct
     1141 cacccttgtg taaccgtaga aaacggtcgc gctgtcacta cgtctgttgc ggttgcagag
     1201 ttcttccaca aacgacacga caatgtgtta cgtgccatcg caaatattga atgctcagat
     1261 aaatttactg ccctcaattt tgaagcgagc gaatacaccg actcaatcgg gcgcaaactc
     1321 ccaatgtacc aaatcaccaa aaacggcttc gttttcctgg ttatgggctt cactggcaaa
     1381 aaagccgctg cattcaaaga agcctacatt gctgagttcg accgcatgga ggccgaactg
     1441 cgccagaata acaccccacc tgctgacaag atgattccgg gtgatggtcg cactctggtt
     1501 gttcacttcg acaaattcgg caatgtcgaa ttcaccgaaa ccgttcctga tggcgcactg
     1561 gtctgtaccc tggagacttt ccggttttat ctggaaaaac agggatggac tcttgttaac
     1621 cggggcgcaa ttaaaaatat gactgtggaa caattactca aaattcattg ttgaggacgc
     1681 aataatggag acgttattac caaacgttaa tacgtctgaa ggttgttttg aaattggtgt
     1741 cactatcagt aaccctgtat ttactgaaga tgccattaac aagagaaaac acgaacggga
     1801 gctattaaat aaaatatgca ttgtttcaat gctggctcgt ttacgtctga tgccaaaagg
     1861 atgtgcacaa tgaatacagt atttgcactc gttctgacgg tttttcttgt ttccggtgaa
     1921 ccggttgaca tggttactgg cgtatacagc tcaatgaaag aatgtatgac tgccgcagcg
     1981 aaacagaaaa ttcccggtga ctgttatccg gtcgataaag ttattcacca tgataataac
     2041 gaaatcccgg caggtcttta aaacagttcc gtaataaata tccggtttca ttcttatatg
     2101 ccagcaatgg caggggttcg ctcaacctta attaaggaga aaaacatgat taccagttat
     2161 gaagccactg ttgtaactac tgatgacatt gttcacgagg ttaatctgga aggaaagcgt
     2221 attggctacg tgattaagac agaaaataaa gaaaccccat tcactgtggt tgatatcgac
     2281 ggtccatcag gcaacgttaa aacacttaac gatggtgtaa aaaaaatgtg tctggtgcac
     2341 ataggaaaga atctgcccgc agaaaaaaaa gccgaatttc tggcaactct gattgcaatg
     2401 aaattaaaag gtgaaatctg aaaaaaagaa agcctgcaca ctgtgcaggc ctgagtgaag
     2461 aacctgggac atttattcat cactcgcagt aattttaatc tgagttgagg ttaaaaaaca
     2521 atgagcaccg ataaacaagt ttacccac
//
LOCUS       DAZPEP010000298         2546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZPEP010000298 DAZPEP010000000
VERSION     DAZPEP010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.300"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..275
                     /locus_tag="ACHUT9_004415"
     CDS             <1..275
                     /locus_tag="ACHUT9_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350712.1"
                     /translation="ITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLR
                     LQDQQEETSVTLFQGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(665..799)
                     /locus_tag="ACHUT9_004416"
                     /pseudo
     CDS             complement(665..799)
                     /locus_tag="ACHUT9_004416"
                     /inference="COORDINATES: protein motif:HMM:NF024729.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(812..1705)
                     /locus_tag="ACHUT9_004417"
     CDS             complement(812..1705)
                     /locus_tag="ACHUT9_004417"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1350713.1"
                     /translation="MSHIRPDFPEPYGLPVDGLRGKTSIFSHPVYLFLREFSLQDSRG
                     GSLSKKAESLSSGKITIGTKEKVIIINELRQCHPLSQLLVIADLPRSTFYYHVKRLNA
                     PDPYQLVKQVILRIYHQHKGRYGYRRIRLACRNEGILLNGKTIRKLMKELGISSLIRR
                     KKYRAYRGEQGRTCNNLLKRQFYADRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEI
                     VSYNLTERPLASMVKSMLLDAVEQLNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQS
                     MSRRGNCLDNAAMESFFSTLK"
     gene            1672..2546
                     /locus_tag="ACHUT9_004418"
                     /pseudo
     CDS             1672..2546
                     /locus_tag="ACHUT9_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611469.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1902..2018
                     /locus_tag="ACHUT9_004418"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          665 a          578 c          598 g          705 t
ORIGIN      
        1 ccataacaga ggccaccctg tacgaaaacg ggctgtatac gcgccacatc agactcagtc
       61 gcccagtatc tgacagcaaa taccccttgc cacccggatc tcagctcatt aaaaatatga
      121 ccttacgact gcgcctgcag gatcagcagg aagaaacatc cgtgacatta tttcagggaa
      181 aaatgagtac cgacggaaat aactattaca tcatcaaagg taaagtaagc tcagtacttc
      241 tgttattaaa aatgctgcag attaatcacg cctgatctct gtcccggtat tttagcccga
      301 ggtatattct gaaatatcat tcatcatgac cggcgtttat acaaacgccg gtcattcgct
      361 aacagcttca ccgttccttc ccccatccag gttccgcatg agcgtgttgt aatgttcata
      421 agcgtattat caggattgtc tggaaaattc ccgatacttt ggactattac tcctgaacaa
      481 ttcgaaattc agcagattaa aggtaagcgt taacggaggt aagcgttaac ggaggtaagc
      541 gttaacggag gtaagcgtta acggaggtaa gcgtaaaccg actgccgtat gtagccatta
      601 agccagtatt ggtaacgtag gtgcccacct tttcaaatga tctgcgcccc aaatcttgga
      661 catcctatga aaaatgttta agtcgatatt gaaccggact catgccatcg agtttctctt
      721 taatgcgatg gtgattgtag tattcaatgt aatcaattat atcttgcttt agatgatgta
      781 acttattgta tttatttagg tagtaacatt cttattttaa agtactaaaa aagctttcca
      841 ttgctgcatt atctaagcag ttaccacgtc gggacatgct ttgtgttatg ccattatcac
      901 tcaaccaacg ttgccaacga ggcatctgat attgccaccc ctgatcacta tgcaacaagg
      961 gcttatcgtc tttattgagt tgttctaccg catctaatag catgctcttt accattgatg
     1021 ctaatgggcg ctcagtaagg ttatatgaga ctatttctcc attatacagg tccatgattg
     1081 gtgataaata gagcttcctg ccatcgacct taaactctgt gacatctgtt acccactttt
     1141 gatttgggcg gtcagcataa aattgtctct tcagtaaatt attacaagtt cgcccctgtt
     1201 ctccacgata ggcacggtac ttcttgcgcc tgataagtga gctgataccc agttccttca
     1261 ttaactttct gatggtttta ccattaagta aaatgccctc gttacggcag gcaagtctga
     1321 tgcgtcgata accataacgc cctttatgct gatggtatat tctcaatatt acttgtttaa
     1381 caagttgata tggatctggc gcattaaggc gtttcacgtg atagtaaaat gtactacgtg
     1441 gcaaatccgc gataaccagt aattgtgata atggatgaca ttgccttaac tcattaataa
     1501 tgattacttt ttcttttgtt ccaatcgtaa tttttcctga gctaaggctt tcagcttttt
     1561 tagataatga accgccccgg gaatcctgga gactaaactc cctgagaaag aggtaaacag
     1621 gatgactaaa aatactcgtt ttcccccgaa gtccgtcaac gggcagtccg tatggttctg
     1681 gaaagtcagg gcgaatatga ctcacaatgg gcggtaattt gttccattac tccaaagatt
     1741 ggctgtacac cggagactct gcgtgtctgg gttcgccagc atgagcggga taccaggggc
     1801 ggtgatggag ggctcaccac cgctgaacgt cagcgtctga aagagctgga gcgtgagaat
     1861 cgtgaactgc gctgcagtaa cgatatcctt cgccaggctt ccgcttattt tgcgaaggcg
     1921 gagttcgacc gcctctggaa aaaataatgc cactgctgga taagctgcgt gggcagtacg
     1981 gggtcggacc ggtatgcagt gaactgcata ttgccccgtc aacgtattac cactgttagc
     2041 aacaggacat catcctgata aacgcagtgc ccgtgcgcag cacgatgact ggctgaagag
     2101 agagatacag cgcgtatacg atgaaaatca tcaggtgtac ggtgtgcgta aagtctggcg
     2161 ccagttgtta cgggaaggaa tcagggtggc cagatgtaca gtggcgcgcc tcatggcggt
     2221 tatgggactt gtcggtgttc tccggggtaa aaaggtccgc actaccgtca gccggaaaac
     2281 cgttgccgca ggtgaccgcg taaaccgtca gttcgtggca gaacgtcctg accagctgtg
     2341 ggtggctgat tttacttacg tcagcacatg gcagggcttc gtctatgtgg cgttcatcat
     2401 tgatgtgttt gccggataca tcgtgtggtg acgagtctca tcgtctatgg aaacgacatt
     2461 cgtgctggat gcactggagc aggcgttgtg ggcccgtcgt ccgtctggca ccatccatta
     2521 cagcgataaa ggctctcagt atgtgt
//
LOCUS       DAZPEP010000299         2545 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZPEP010000299 DAZPEP010000000
VERSION     DAZPEP010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.301"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(1..787)
                     /locus_tag="ACHUT9_004419"
                     /pseudo
     CDS             complement(1..787)
                     /locus_tag="ACHUT9_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
     gene            complement(840..1436)
                     /locus_tag="ACHUT9_004420"
     CDS             complement(840..1436)
                     /locus_tag="ACHUT9_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFR1350714.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(1463..2065)
                     /gene="yadL"
                     /locus_tag="ACHUT9_004421"
     CDS             complement(1463..2065)
                     /gene="yadL"
                     /locus_tag="ACHUT9_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFR1350715.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGLANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            complement(2080..>2545)
                     /locus_tag="ACHUT9_004422"
     CDS             complement(2080..>2545)
                     /locus_tag="ACHUT9_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1350716.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          707 a          551 c          508 g          779 t
ORIGIN      
        1 taagcatagc ggtaaaacaa gtgggattag tcaacttgac attgagcaga tcgaaatcga
       61 ttttgataaa ataactatgc tcatttatac cttggcctgc gccataggct ttaaatgaat
      121 acagataatt tgatctgttg agttactgtt ggatcaaagt ttgtatctgt atagaattca
      181 attgtaaggg tctgataaat accacctaca gcccagtatt tacgggtgct attcgcatta
      241 ttgcaactcc aatataacac gtcttcgctt ggattatacc acgaaaaata ttgttctgca
      301 gagtcaccaa tgtatatccc aggtgaacta acgttagtta cggtaccgta agctgaccac
      361 atgtttgaaa ttaataatgt gtaatacaaa ccaggaactg atgtattaaa taatttatgg
      421 gagccataca ttttacctga gtcaaccatt gcattttcaa tacgcattgc gctaccactt
      481 gtgctatcac ttctgttgca ataaagaaaa ccaccaccag aagcggtatc taactgactc
      541 cactgtaatt ttacacctgt aggtccaagc tgcgtcggtt tgtagaaaac taaattattt
      601 gtacttgttg catatctgaa tatgacagta gcatcgccat tcggccccgt ttctctccat
      661 ttttgatctg acggaccaac gccgtaacta ttcccaacaa tatatcctac gttatcatca
      721 gtgccagcac tcgcggtata acaactcaag cacagaatta aaaaaaatgt ctgaagaatt
      781 tcttcattat cactccacca tctactgctg taacagtcgc agttatttgt tttgatctat
      841 tattcgtagt acacatcaac taatatatga ctcattactt ggccagatat gacatccagc
      901 gcgggatcga ttttttgcat tctggcataa aatttccatg aagaattatg cagggcagaa
      961 gtcattactg gatacacgga acgagaattt cctgctgaac taataacatt gaatatattg
     1021 gtcgggtcct gaatggaaaa gagaacgaca ccgacatttt tagccccggt tgcgacttca
     1081 ttggcaaata tttgccttga acctgcagcc atgacaccgc taagggctct aaaatcaata
     1141 tgtaactgat taagcacatc gccagttccc tgtaatgcac agtctgatac tgtgacagtg
     1201 aactcttttc cacctggttg atagctttca ggagtaacat tatctgaaaa atatcccacc
     1261 ccaacgacgc caagatcaat attaccctgg ttactgattc cgcttttgca agtgctgttt
     1321 tttacattgg caacaaggtc aatatcctgc ccagcgatgg ctggataggt aatgggaaac
     1381 tgtagtgaca gaaacagaat tattctcttc atcagcttac gttgagtggg atgcatatat
     1441 agttcctgga tctgtcagtc tgttagttat aggttactgt aaatgtggca ggtgcagaaa
     1501 atttacccgc agtgacattg cttacggttt tatttttttc gacgaccagt cgagcactca
     1561 tcggataata aacaaccgcg ttggctgcat tggagatagt gacctcttga gatgctggtg
     1621 tcacacaact aaattgtgtt gcgctccctg ttgccggagt ttcggtgctc cagacttcaa
     1681 cggcgacggc acttgcttta tctgcggctg tggatccatt tgcgagcccc gcaccgtcat
     1741 tagcagtacc ttcgcatgtg gctcgcttgg ttaattttat ttgtgctttt ttattgggga
     1801 tacccgcaca gtctttgaat ttgagtttga atgttttaac tttggtcttg gcattgattt
     1861 cagaaatata tacatcgcca aagtcaacaa cagaaatgtc ctgatcagag tcatttacta
     1921 atgtagcggt acaagtacct atttctacgg tagtaatcac ggtcagccca atgctgtctg
     1981 ttgccgcata agagggtacg ctgaacaatg aggcagccaa aacgacactg ctggataatc
     2041 cataacgtaa atttttaaat gtcatcttaa ttatccttct tattgataga tgaaattaaa
     2101 cgttgccagt gcacgaaaat tccccggtac gccttgccct gcatctgttt cacgtagcgc
     2161 aacggtcatt tcaagaccct tatcgggctg catctcgtct gtgctccagc gtatcttttc
     2221 cgcactgtta ggtttaagga aagtggcatc atcagtagtc cgttttttga aacccatacc
     2281 gatattactt gtcgtcgaag atgaatcacc agactgcggt ataataagct taggtgagct
     2341 tgatgatgca tttccggtca gagtggtatc aatccaactg atgccactgc tgcacccact
     2401 agcaaccagt ttaaaatcag cctgagattc tgtcgtttta ttcgcgatct tatccagacc
     2461 catcttaggg attctcaatg tgtagttatt attgccatca ttcgtgacgt tattaccagt
     2521 aagtgtgatg ttacaggttg tcgct
//
LOCUS       DAZPEP010000300         2542 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZPEP010000300 DAZPEP010000000
VERSION     DAZPEP010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.302"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(108..1526)
                     /gene="phrB"
                     /locus_tag="ACHUT9_004423"
     CDS             complement(108..1526)
                     /gene="phrB"
                     /locus_tag="ACHUT9_004423"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFR1350717.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1523..2032)
                     /locus_tag="ACHUT9_004424"
     CDS             complement(1523..2032)
                     /locus_tag="ACHUT9_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFR1350718.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(2459..>2542)
                     /locus_tag="ACHUT9_004425"
     CDS             complement(2459..>2542)
                     /locus_tag="ACHUT9_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350719.1"
                     /translation="AGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          642 a          660 c          580 g          660 t
ORIGIN      
        1 tcaggcctac gtatttcctg caatttattg aatttgcaca aatttgtagg ctggataagg
       61 cgttcacgcc gcatccggca tctgggctcg attgcctgac gcgtctgtta tttccccttc
      121 cgcgccgcct catacgctgc caacgtttgt actctcgcct ctttgtgctc gactatcggt
      181 tgcggataat ccagcttcac acctgctttc tgcgcccact tccacggctc atgcaccgat
      241 ttccccggta cattgcgtag ttccggtagc cactggcgga taaactcacc ttcgagatca
      301 aatttctcgc cctgggttgt cgggttgaaa atacgaaaat acggcgctgc atcggttccg
      361 gttgaagcgg cccactgcca gccaccgtta ttggctgcca aatcaccatc aatcagctgc
      421 gacatgaaat atcgctcgcc ttcgcgccag tcgatcaata aatctttcac cagaaaactg
      481 gctgtaatca tccgtagcct gttatgcatc cagccagtgc tgttaagctg acgcatagcg
      541 gcatcaacaa tcgggtatcc cgttttgcct tcctgccagg cctgtaaatg tgcgggattg
      601 ctctgccact gtacacgatc cgtccaggca ataattggac gatgtttaca caacgagggg
      661 tgatacgtta tcaggtgacg gtaaaactcg cgccagatca gctcattaag ccagacacta
      721 ccgaccccac cgtccagcgc ctgcggctgt tcagccaaca agcgatgcaa gcactggcga
      781 ggcgataacc cgcccgttgc caggctggcc gacaaacggc tggtgccttc cactgccgga
      841 aaatctcgtt gttgctcata ttctccggca ccgttctggc aaaactggcg taattgcgca
      901 atcgccgctt tttcttccac cggaaaatgc gcagtatcga aagactgacg aggataattc
      961 agcgtaatgg atggcgaggg ctctatcgat ccgctactac gaacttttgg cgcagcgacg
     1021 cactccggca tcccttcccg cagccgtttc agccaggcat tcttaaaagg cgtaaagact
     1081 ttgtacatct cgtgattacc ggtcatcacc gcgccaggcg gcaggatcac gctgtcatca
     1141 aatccttcac acaccacgtt acgcagcgct ctttcaactt ccacatcccg cgcccgctca
     1201 ttcacttcat actgatagtt ataaaacagg tgggtaacgc tgttttccgc gcacacctgt
     1261 ttaactattt cgacactggc gacaaagtca tccacttcac ggaacaataa aggaatacct
     1321 ttttccgcaa gcgctatttg tagcccattc agttgagcat tgataagttc agcctgacgc
     1381 ggcgacatgt tatgcgtcgc ccactggcgt ggtgtagcga tatacaacgc cagcacgcgt
     1441 gcagacgaat tgcggcaggc ggcagccagt gcgagattat cgtgcagacg taaatcttgg
     1501 cgaaaccaga ccagatgggt agtcataaaa ctcctgaatg gcgcaagtta atcacacgcg
     1561 gccaaagttc gaaataacgc tgccctgaaa gccaggcgtc atgataaagc gccatatagt
     1621 gtttgatacg catcagcggc gcaagaagtg gttgctcgcc acggcgataa ctgtcgataa
     1681 gcgcagccag ctgctggcgt tctgtggaat caatatgcgg gcgaaaataa ccctgaacgt
     1741 gcatcaaaac attggtgtga tcgcgcgggt tggcggggtg agaaagcaaa acaactacgc
     1801 gttggcggta ctggttgtaa tagtcatcca gattttgcca ctggtgaata tcagccacga
     1861 acgggccaag tttgcggtac tccggctgag aatgcgccag aaagacgagt ttatagcggc
     1921 tgtgataatc gagaagcgcc ccgcgcgtta agccatgttc tttcagtcga tgtaactcat
     1981 gaagggcaaa gatacggatt agggtgctgt catcaaatcg ttgtagattc attactgctc
     2041 ctgaacggta taaaggagta agtgtagacg atggtaaatg aattttatgg ggcagagttt
     2101 ttgcggggaa gtattttgtt ggtaaaaata ttatcaactt catctgagtg agaattattc
     2161 tactatttcc cgagctgcat tcattttttg tattttttat ttttagaaaa taacagttct
     2221 caaagcttaa ttacctcact attaattgta tttcaaacta acaatcgcac tccattttct
     2281 ctttttaaaa aacataaaca ctgttaaaac aaatacttat ttcgttatct taaatgctat
     2341 ttctacatca ctcgtcctta taatcaaatt attcttattc atagctattt ttgcaggttt
     2401 aaaatagcgg ctttcaccaa agtcagccat gaaaattaga ctacaagtca gggcaagatt
     2461 acgaaagccc gctccccgca aggactgacg ccagatagtt tctgtccatg gctgcttttc
     2521 gcatcttacg tcttaaccct gc
//
LOCUS       DAZPEP010000301         2541 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZPEP010000301 DAZPEP010000000
VERSION     DAZPEP010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.303"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(14..88)
                     /locus_tag="ACHUT9_004426"
     tRNA            complement(14..88)
                     /locus_tag="ACHUT9_004426"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Gln,seq:ttg)
     gene            complement(112..196)
                     /locus_tag="ACHUT9_004427"
     tRNA            complement(112..196)
                     /locus_tag="ACHUT9_004427"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(160..162),aa:Leu,seq:tag)
     gene            complement(205..281)
                     /locus_tag="ACHUT9_004428"
     tRNA            complement(205..281)
                     /locus_tag="ACHUT9_004428"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(245..247),aa:Met,seq:cat)
     gene            complement(661..2325)
                     /gene="asnB"
                     /locus_tag="ACHUT9_004429"
     CDS             complement(661..2325)
                     /gene="asnB"
                     /locus_tag="ACHUT9_004429"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFR1350720.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            complement(2279..>2541)
                     /locus_tag="ACHUT9_004430"
     CDS             complement(2279..>2541)
                     /locus_tag="ACHUT9_004430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350721.1"
                     /translation="PVKYNHSIKPAKNYRFINKYILTPAPFSPIDNHHYVFYFFTAKS
                     LAHSAALRHFFKQANTTSNKYQVNGEGYVFNFWRIRYQNRRS"
BASE COUNT          610 a          639 c          630 g          662 t
ORIGIN      
        1 attgtttctt cgatggctgg ggtacctgga ttcgaaccag ggaatgccgg tatcaaaaac
       61 cggtgcctta ccgcttggcg ataccccatc cgtacaacgc tttctggtga atggtgcggg
      121 aggcgagact tgaactcgca caccttgcgg cgccagaacc taaatctggt gcgtctacca
      181 atttcgccac tcccgcaaaa aagatggtgg ctacgacggg attcgaacct gtgaccccat
      241 cattatgagt gatgtgctct aaccaactga gctacgtagc catctttttt tcgcgatacc
      301 ttatcggcgt tgcggggcgc attatgcgta tagagccttg cagcgtcaac ctctttttca
      361 aggaaaattg ctcgaaagtg actgtttggt taggttgcga acagcgtggc gctatattcg
      421 tcaattattg tttactttgt gttttttccc caccctacag ccattctttt gtcatacagg
      481 atgaaattcg gaattcaaca atggtggtgg gaaattaatc tatgaaatac tggcctacag
      541 tgatgagttg tcaaacagtg atgtggcaaa cccggaacat ttccttactg catatcagaa
      601 tcaacaagca cctcaataac tgaaacagcc ccggatttca ccggggctgt ttcgcatttc
      661 ttacttatac gccgactggt gaacgccaac cgcacgacca gacggatcgt ccattttctt
      721 gaacgcctca tcccactcaa tcgctttagc ggaagaacac gcgacggacg ggccgcccgg
      781 cacgcactca gcggcgctcg gaagcgggaa cagttcttca aagatctccc ggtacagata
      841 cgcttctttt gaggttggcg tgttgtacgg gaagcggaag cgggcagttt ccagttgctg
      901 atcagaaacc tgctgcgccg ccacttcttt cagggtgtcg atccaactgt aaccgacgcc
      961 atcggagaac tgctctttct gccgccaggc cacgcttgcg ggcaggtatg actcaaaaca
     1021 ttcacgcagg atgtgttttt ccattttgcc gttaccgcac attttatcct gcgggttaat
     1081 gcgcatcgcc acatcaagga attttttgtc gaggaacgga acgcgtgctt ccacgcccca
     1141 ggctgacatc gctttgttgg cgcgcgcgca gtcatacata tgcagggcca gcagtttacg
     1201 caccgtctct tcatgcagtt ctttagcgtt gggcgcttta tggaagtaaa gataaccgcc
     1261 aaacacttca tcagaacctt caccggacag caccatttta atgcccatcg ccttgatctt
     1321 acgcgacatt aaatacatcg gtgttgaagc gcgaatagtg gtcacatcat aagtttcgat
     1381 gtggtaaatc acgtcgcgga tggcatccag accttcctgt acagtgaagt gaatttcgtg
     1441 atgcaccgtg cccagatggt ttgccacttc ctgggctgct ttaagatccg gtgaacccgg
     1501 cagacctaca gcaaaggagt gtaactgggg ccaccaggct tcagagcgtt cctgatcttc
     1561 cacgcgacgg gctgcgtatt tcttggtgat agcggaaata attgaggaat ccagaccacc
     1621 agaaagcagc acaccgtaag gcacatcaga catcagatgg cttttaacgg aatcttccag
     1681 agcctgacgc agctcgtttt tgtcggtcac gttatctttc actgcatcaa agtcgaacca
     1741 gtcgcgatga taataagaac ggatttcgcc gtcctggctc cacaaatagc tccccgccgg
     1801 gaactcttta atcgtgcggc aaactggcac cagcgatttc atttctgagg ccacatacag
     1861 ctgaccgtgt tcgtcatacc ccatatacag tgggatgatc cccagatggt cgcgaccaat
     1921 caggtaggca tctttttcgc tgtcgtacag ggcaaaggca aacatgccct gcaagtcgtc
     1981 gagaaattcc ggcccttttt cctgatacag cgcgaggatc acttcacagt cagaccctgt
     2041 ctggaactga taacgatcgc catattcagc gcgcaatgcc tggtggttgt agatttcacc
     2101 gtttaccgcc agtacgtggg ttttttgttg gttgtagaga ggttgcgccc ccgcgttaac
     2161 gtcaacaatt gacagacgtt cgtgagcgag aatggcgtta tcgctggcat aaataccgga
     2221 ccagtccggg ccacgatgac gcatcaggcg tgacagctcg agtgctttct tacgctgctc
     2281 aactgcgtct gttttgatat cgaatacgcc aaaaattgaa cacataacct tctccgttaa
     2341 cctggtattt gttgcttgtt gtgtttgctt gtttaaaaaa atgccgcaaa gcagcactgt
     2401 gcgcaagcga tttggcggtg aaaaaataaa aaacgtaatg gtgattgtcg attggtgaaa
     2461 aaggggctgg tgtgaggatg tatttattga tgaatcgata atttttagcg ggttttattg
     2521 aatggttata ttttacgggg g
//
LOCUS       DAZPEP010000302         2439 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZPEP010000302 DAZPEP010000000
VERSION     DAZPEP010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.304"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHUT9_004431"
     CDS             complement(<1..458)
                     /locus_tag="ACHUT9_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1350722.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTSDTALLKSFEAFREWVAIQAGFYTEHFYPDGSRGCRAKSIA"
     gene            complement(497..784)
                     /locus_tag="ACHUT9_004432"
     CDS             complement(497..784)
                     /locus_tag="ACHUT9_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350723.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHUT9_004433"
     CDS             complement(1034..1189)
                     /gene="hokD"
                     /locus_tag="ACHUT9_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFR1350724.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1417..2082)
                     /locus_tag="ACHUT9_004434"
     CDS             complement(1417..2082)
                     /locus_tag="ACHUT9_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFR1350725.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2251..>2439)
                     /locus_tag="ACHUT9_004435"
     CDS             complement(2251..>2439)
                     /locus_tag="ACHUT9_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350726.1"
                     /translation="CGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQ
                     IERNAKARIRRATKSRTE"
BASE COUNT          608 a          640 c          543 g          648 t
ORIGIN      
        1 gcgatagatt tcgcccgaca cccacggcta ccgtccggat aaaaatgctc ggtgtaaaat
       61 ccggcctgaa tggctaccca ctcgcggaaa gcctcaaacg actttagcaa tgccgtatca
      121 ctggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc ggcctactga ttcacacagg taatcaacga aaccggacac cagttttcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaaacccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cacacacgct taaagtctgc gtgtatccac
      361 tcacctattt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgac cagttgtata tgtgccatcg gctttctccg gtggcacggt
      481 gttacacagc aggagttcaa tcctgctcaa gattgtagat gagtttattc ttcagcaaaa
      541 gcagaaaacc agccttaaaa ccaatctctt tcaaaacccg taatgatgtg acaaattcgt
      601 cctcacgcaa aataaaaccg tccgtcagaa gtccattaca aaaataaaat aacacagcac
      661 cgctcttcct ttgttgagat tgcaaacatc taatgcggca atggctgaca atcgctccat
      721 tctcaacgcg cacagcatag aggccatttt cactaaaaat ttcacgcaat tcttcgattt
      781 tcatcttcag aatccttcca gataaatagc tctcccctgt tcggggtcca tccctcttct
      841 ccctgcgcgc tacttaagtg agtcgattct atctgcgaag gtgcgcgaat caaattcacc
      901 ggaaataaac aacaaaaaac ccgccgaaac gctgagagat cacagcgatg ggcgggttaa
      961 gtgcgggtgc gttgaggatg cctgacacat cagaggtggg cggggatggg atcagctccc
     1021 cgcccggtca ctcttacttc ctggattcgt agtctacgaa gacagcgacc tccgtctggc
     1081 cggttcggat tcgtacctca cagaggtctt tcctcgttac cagtgccgtc actatgacgg
     1141 ttaaacagat gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct
     1201 ccttgccttt cggcatgtaa gaggctaacc tacatgtgca aagcatgaaa ttggcctcag
     1261 attaatgtta agcgtcttgc cggacgcgta atgttaactg gggcttttct ctatctgcct
     1321 tttggtgttc atgcctgaag cagatagcct caagcacccg cagtcattct acttacctcg
     1381 ccaatatgaa atcaatcaga aaggtgcccc ataaaatcac tccttctctt ctttaccgta
     1441 gtggagttga ccaattttga taagagggcg tccctgagat ttgcggtgta gattggtatc
     1501 gcgcagagaa tacacacagc cacaatattc ctgctgatag aatttttcgc gcttgctgat
     1561 ttcaatcata cgggacgagc cgccctgctt gcgccagtta taatcccagt acaccatgcc
     1621 cggataatgc gcgacggctc gccgcccaca gtcgttaacc tgctgcatat ttttccagcg
     1681 tgaaatgccc agtgaactgc tgatcacact gaaaccattt tcagcggcgt acagcgctgt
     1741 ccgctcaaaa cgcatgtcaa aacacatggt gcaacggatc cctcgttcgg gctcccattc
     1801 cattcctttg gctcgttcaa accagttgtc tgtgtcgtaa tcagcatcaa taaacggcac
     1861 gccgtgttgt tcagcaaagc gaatattctc atccttacga attaaatact ctttctgagg
     1921 atgaatgttc gggttgtaga aaaagatggt gtagtcgatt cccgaggcct gaagcgcctc
     1981 catcacctca ccggaacatg gagcacagca agagtgcagt agtagtttgt ttgccccgtt
     2041 tgggagctcc aatttgggcc gtttgaaatc agcaactgtc ataaatatgt ttattggggt
     2101 cataaaaata tcaaagagtg tagcattaga acggggctat cggaaacaga tgtgtgactg
     2161 ctccccgccc tttcgggtgg tctcctgatg atttgagggt gcagaaatcc ctccggttaa
     2221 ggattaaatt tttaacagtg ctaaatttaa ttattcagtt ctggattttg tcgccctgcg
     2281 tatccgcgct ttcgcgttac gctcaatctg tatcagcttt tctatatttc tccgcctttc
     2341 ctgttcctcc tggcgcaata gccttacatc atctgccagt ctggtttctc tttttgccac
     2401 agagagcatc cagtcaaacg gctccacaac cgcaccgca
//
LOCUS       DAZPEP010000303         2331 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZPEP010000303 DAZPEP010000000
VERSION     DAZPEP010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.305"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(25..315)
                     /locus_tag="ACHUT9_004436"
     CDS             complement(25..315)
                     /locus_tag="ACHUT9_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFR1350727.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHUT9_004437"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="ACHUT9_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFR1350728.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="ACHUT9_004438"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="ACHUT9_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFR1350729.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHUT9_004439"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="ACHUT9_004439"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2331)
                     /locus_tag="ACHUT9_004440"
     CDS             complement(2171..>2331)
                     /locus_tag="ACHUT9_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1350730.1"
                     /translation="VKLSMPELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLG
                     TIRAFDVT"
BASE COUNT          643 a          621 c          516 g          551 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc t
//
LOCUS       DAZPEP010000304         2330 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZPEP010000304 DAZPEP010000000
VERSION     DAZPEP010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.306"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            20..238
                     /locus_tag="ACHUT9_004441"
     CDS             20..238
                     /locus_tag="ACHUT9_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFR1350731.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="ACHUT9_004442"
     CDS             240..605
                     /locus_tag="ACHUT9_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFR1350732.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="ACHUT9_004443"
     CDS             602..1267
                     /locus_tag="ACHUT9_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350733.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="ACHUT9_004444"
     CDS             1267..1632
                     /locus_tag="ACHUT9_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFR1350734.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="ACHUT9_004445"
     CDS             2087..2242
                     /locus_tag="ACHUT9_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFR1350735.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          495 c          628 g          580 t
ORIGIN      
        1 ctcgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggttt
//
LOCUS       DAZPEP010000305         2308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZPEP010000305 DAZPEP010000000
VERSION     DAZPEP010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.307"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..185
                     /locus_tag="ACHUT9_004446"
     CDS             <1..185
                     /locus_tag="ACHUT9_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350736.1"
                     /translation="TKIKTTIITICALSYNLTAQASPSYRPRDRVNKTMIELQTNGVE
                     RQLLDNSCGISSGVFK"
     gene            667..1014
                     /locus_tag="ACHUT9_004447"
     CDS             667..1014
                     /locus_tag="ACHUT9_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFR1350737.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1034..1624)
                     /locus_tag="ACHUT9_004448"
     CDS             complement(1034..1624)
                     /locus_tag="ACHUT9_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350738.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(1621..1881)
                     /locus_tag="ACHUT9_004449"
     CDS             complement(1621..1881)
                     /locus_tag="ACHUT9_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350739.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            1979..2283
                     /locus_tag="ACHUT9_004450"
                     /pseudo
     CDS             1979..2283
                     /locus_tag="ACHUT9_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          569 a          608 c          566 g          565 t
ORIGIN      
        1 acacaaagat aaaaacaact atcattacaa tatgtgcatt atcatataat cttacagcgc
       61 aagcttcccc gtcatacaga cctcgtgatc gagttaataa gactatgata gaactacaaa
      121 ctaacggagt agaacggcaa cttttagata attcttgtgg cataagttct ggggtattta
      181 aataaaatat aagacaggct gtctatctta cagacagcct ttttataata acagaaagaa
      241 tatatatcat acttaacgga agagataata tgaaaacagt acccgtataa acagcaagca
      301 atcccatttt tccaggtgta tcagcaaaaa aaccgctgtt ctagaaatca ggccgggtaa
      361 atttcagagc tgtatcttca atataccatt ttgcaaccgg atacaaaacc attccgcaga
      421 gagaaataca ccagaacagt aatctgtatt taaaatcata atcccatgac atatacagca
      481 tatatcctcc cgtcacccat ccccaccaca tattattaaa ataatatttt ctgttcatct
      541 gccagttttc ctttcttttg cacttttgtc agtgtactga tgcatgacaa cataaccaca
      601 gcatgataat aataatcaat aacaataagc tgtgtcacgt ttacatcatg gaacggaggt
      661 gctcctgtga cttttgagca gaaaaaagcc cgggccattg ccctgatgga cagcaaaaag
      721 atgtggcgga gcaattatgc ccctcctctt ctgcgtattc tctggcggct gggtatccgg
      781 cttccgccat taccctttat gccgttctgg caggtcacgg tgctgacggg gggcttatgg
      841 ggcatttcct ggggatgtgc aatgtggttt atctactggg ggccgtcagg aatggtggca
      901 ggtgaggcca ttattataag tatcacgggg ggttttttgt ccggactgct catggcctct
      961 ttccactggt ggcgcaggaa agttaaccga ctgccaccat gggatgatgt gtaaaccgtg
     1021 cacaggcgtg ttcttacgga gcggcacttt catttatctg tacgcgaacc tccattcctg
     1081 catcgtgaca ggcctgcagc cactgcgcca cttccagcgg atcgccctcc cggcgtacca
     1141 cccagccttc tttattccat aactgcagac aggtgctgcc gtcgagacgc accacaaaat
     1201 ccccacggca ggcctgatac gttatgccgt tcagctccac cgccagatca gtggcagaag
     1261 tgccgcctgc gggcagacct gctgccggga ccagcggcag tacgacgact tctgccgcac
     1321 gctgttctgc ccggatgcgg tcctgttctg ccagcagcag aggctgtgcc acggcccggc
     1381 cggcgtccgt cagctccact gccagctgca ggttcggagc gcgcagggta cgcagccagc
     1441 cggcatcctc cagacgacgg caggaggcgc gcaggtttgg cccgtagacc ggggcttccc
     1501 cgctttgctc cagcacccgt tctatgtccc gcgtcagcac gggtcctggc cgtttttcat
     1561 caagggcagc cagcacaatc agcactctcc gctgcagtgg cgacggtcgg cggctctgag
     1621 tcatttccag tcatcctccg gccagatgag ctttctgagg cgctgatggt cggcgtcaat
     1681 gatctcctgt ctctttatgg cattcattga ggtatagcca tttatggcca gttcatgcca
     1741 gaagatcagc acggctccgg ctttggctga ctcgcagaga taagttacgc cgtggctgtc
     1801 cagattgcat tcccggatgc gtttttccgc aaaccgggtc agttgttcat agctgagtga
     1861 aaacgtcatt tcctgatcca tcctgcacgt tccatctgat aatgttctga caggtaaaaa
     1921 tcattgaacc gccccgggaa tcctggagac taaacttcct gagaaagagg taaacaggat
     1981 gactaaaaat actcgttttt cccccgaagt ccgtcaacgg gcagtccgta tggttctgga
     2041 aagtcagggc gaatatgact cacaatgggc gacaatttgt tccattgctc caaagattgg
     2101 ctgtacgccg gagactctgc gtgtccgggt tcgccagcat gagcgggata ccgggggcgg
     2161 tgatggaggg ctcaccaccg ctgaacgtca gcgtctgaaa gagctggagc gtgaaaatcg
     2221 tgaactgcgc cgcagtaacg atatccttcg ccaggcttcc gcttattttg cgaaggcgga
     2281 gtttgaccgc ctctggaaaa aatgatgc
//
LOCUS       DAZPEP010000306         2200 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZPEP010000306 DAZPEP010000000
VERSION     DAZPEP010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.308"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..448)
                     /locus_tag="ACHUT9_004451"
     CDS             complement(<1..448)
                     /locus_tag="ACHUT9_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFR1350740.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDF"
     gene            complement(533..1558)
                     /locus_tag="ACHUT9_004452"
     CDS             complement(533..1558)
                     /locus_tag="ACHUT9_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HFR1350741.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            complement(1584..>2200)
                     /gene="rfaY"
                     /locus_tag="ACHUT9_004453"
     CDS             complement(1584..>2200)
                     /gene="rfaY"
                     /locus_tag="ACHUT9_004453"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFR1350742.1"
                     /translation="EILAYNFKTVKVLRNINDTKVSLIDTKYGRYVFKVFAPKTKRNE
                     RFLKSFVKGDYYQNLIVETDRVRSAGLTFPNDFYFLAERKIFNYASVFIMLIEYVEGV
                     ELNDMPIIPEDVKAEIKASMEKLHALNMLSGDPHRGNFIVSKDGVRIIDLSGKSCTAE
                     RKARDRLAMERHLGIANEIKDYGYYSVIYRTKLRKFIKKLKGKA"
BASE COUNT          690 a          442 c          354 g          714 t
ORIGIN      
        1 aaaagtcgaa aagtaaagat ccttcattaa actctgtatt tataggtgag taagtaacac
       61 catcttttat ataatggcca taaagcccac aataaatcgc atcccctccc gtctcatgtt
      121 gtctggtata catcttcagc agatagttag gatctatctc atcatcatca tccataaaac
      181 atataaactg tccttgcgcc ttgcggatac ctgtattcct ggcagacgaa acaccgccat
      241 tttctttatt gataataatt aatcgagagt gggttaaccc atactctctc aaaacgtttg
      301 atgtgttatc tttagaacca tcattaacaa taacaatttc aaaatttggg taagtttgtt
      361 tttctaattt cgttagagtt tcagtaatgt aatcagatga attatacgtc ggtattatta
      421 tggatactaa agggtaatca ttgctcatcg tgttctcttg cctgaactaa taaaatatga
      481 tagtactcat ccttaattat tattgtaact cagacatcca tgatctttat tattatccaa
      541 atagttttct tttatactta tttaatttaa atttaataaa gtaaataaaa gcgttaaacg
      601 tatcaccatt tttctttgca tgttgatatt tcacttgata ctgcttttca ttcgtggctg
      661 gaataaacgc cacatcattc cagcaagaag cctgataagc ctcattaaaa tacttctggc
      721 agggataatt cgtccagtcg ttccagggct tcgttacgcc aacataatga atcagcttag
      781 tatcatcggt aatggtcttt ttataatctt catcgctttt attttttaac tcataatcga
      841 tgccataaaa gcaatcataa tcgccgctaa taaaaataca atgaccaaag aacagaatat
      901 ttaaaatatc ctgatcaagg catttatact tcgtgtgctg cgggtcactt gccagcatag
      961 caatggcttt attcataaaa tcatttttcg cccatgcttc aagctgcaga tatactacac
     1021 cggcgttgaa ataattccct ggaagtccct ctatagccag acgcttcgct ggtttttcct
     1081 gcataaaagg cacatcttta ataacagctg caaacttatc cccatcaaaa ttaatatcaa
     1141 gcaatccagc taatgagcct ttacagatga catcagcatc gatatataac agattttcaa
     1201 tagtttcact cagatactgg aacgcaacta aacggaagaa tgttgcataa gaccactgtt
     1261 tcaggctggg gaagatatca ataaaggcac tggagacttt ataaattctt acttcaacgt
     1321 tagcattttt gctggtaaaa gcattaacac gttgtagata atcttcatca atgtagtcgg
     1381 taaagagatg aaacttaacc gcaaggtccg gattatgcat aacaactgat tgcatagata
     1441 ctgccgcacc aaataaaaag ttacggtcaa taccgtaagc gatatttaac acacgatcgg
     1501 tgttagcaat ttccccagag acagcgactt cagtaatact ctcagctaat aaatccatgt
     1561 tggttccgtt ttgactgtgt ggtttacgct ttgcctttta attttttaat aaatttgcgt
     1621 agtttggttc tgtagattac tgaatagtag ccataatctt ttatttcgtt ggcaataccc
     1681 aggtgacgtt ccatggccag acgatcacga gctttacgct ctgccgtaca gcttttaccg
     1741 gataggtcaa ttatcctgac accatcttta ctaacgataa agttaccccg gtgtgggtcg
     1801 cccgatagca tatttaaggc atgcaatttt tccattgaag ccttaatttc tgctttaaca
     1861 tcttctggaa tgatcggcat atcattgagc tctacgccct cgacatactc gataagcatg
     1921 ataaaaacac tggcatagtt gaaaatttta cgctcagcca gaaaataaaa atcattagga
     1981 aaagtaagac ctgcacttct gacacgatcg gtttcaacga tcaaattctg atagtagtca
     2041 ccttttacaa aagattttaa aaaacgttca ttccttttcg ttttcggggc aaagacttta
     2101 aaaacatagc gaccatattt cgtatcaatc aatgatactt tggtatcatt aatattacgt
     2161 aatactttta ctgttttaaa attataggcc agtatttcat
//
LOCUS       DAZPEP010000307         2136 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZPEP010000307 DAZPEP010000000
VERSION     DAZPEP010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.309"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..784
                     /locus_tag="ACHUT9_004454"
     CDS             <1..784
                     /locus_tag="ACHUT9_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR1350743.1"
                     /translation="KSRRRGAIHVISAFSTMYSLVLGQIKTDEKSNEITAIPELLNML
                     DIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPEH
                     DSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPE
                     MTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGI
                     RHITINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1042..>2136
                     /locus_tag="ACHUT9_004455"
     CDS             1042..>2136
                     /locus_tag="ACHUT9_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1350744.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVMDGAGRRFHLVLTTQAQRAEEARKPHTASLSSPDSPCPL
                     SAPSFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVY
                     DRSGMQVRGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          543 a          496 c          651 g          446 t
ORIGIN      
        1 taagagtcgt cgcaggggag cgattcatgt cattagtgcg ttctcaacaa tgtacagtct
       61 ggtcctcggg cagatcaaga cggatgagaa atccaatgag attacagcca ttcctgaact
      121 tcttaacatg ctggatatta aaggaaaaat catcacaact gatgcgatgg gttgccagaa
      181 agatattgca gagaagatac aaaaacaggg aggtgattat ttattcgctg taaaaggaaa
      241 ccaggggcgg ctaaataaag cctttgagga aaaatttccg ctgaaagaat taaataatcc
      301 agagcatgac agttacgcaa tcagtgaaaa gagtcacggc agagaagaaa tccgtcttca
      361 tattgtttgc gatgtccctg atgaacttat tgatttcacg tttgaatgga aaggactgaa
      421 gaaattatgc gtggcagtct cctttcggtc aataatagca gaacaaaaga aagagccaga
      481 aatgacggtc agatattata tcagttctgc tgatttaact gcagaaaagt tcgccacagc
      541 aatccgaaac cactggcacg tggagaataa gctgcactgg cgtctggacg tggtaatgaa
      601 tgaagacgac tgcaaaataa gaagaggaaa tgcagcagaa ttattttcag ggatacggca
      661 cattactatt aatattttga cgaatgataa ggtattcaag gcagggttaa gacgtaagat
      721 gcgaaaagca gccatggaca gaaactatct ggcgtcagtc cttgcgggga gcgggctttc
      781 gtaatcttgc cctgctgaag agtttgaaag ggcacggatc ctttcaaaag aatacattga
      841 gaaatatata agctaaagat gattagctgt atttcatgat aataatctaa tgagggattt
      901 ttctgaacat caaattgcat cgaagttagt tagagtgctg cgattgtctt gcacgtaata
      961 aattcagaga gaaaatatag ttgtgggtat gagaaaataa ccaacctaat gtaaatcaat
     1021 tcagatgaag aggaataaac catgagcgga aaaccggcgg cgcggcaggg cgacatgacc
     1081 cgcaagggac tggatattgt gcagggttca gcgggtgtgc tgataggtgc gccgacggga
     1141 gtggcctgtt cggtgtgtcc gggagggatt acctatgcta acccggtgaa cccggtgctg
     1201 ggtgcgaagg tgctgccggg cgagacggac cttgcgctgc cctgcccgct gccgtttatt
     1261 cttttccgcg cctacagcag ctaccggacc agaacacccg cgccggtggg ggtgtttggt
     1321 cccggctgga aagcgccctt cgatatccgc ttacagatac gcgatgaagg cctgatactc
     1381 aacgacagcg gcggcaggag cattcacttt gagccgctgt ttcccggcga gataagctac
     1441 agccgcagcg agtcactctg gctggcgcgg ggcggagtag cggcgcagca cagttcgcag
     1501 ccgctaagcg cgctctggca ggtgctgccg gaagatgttc gcctgagtcc gcatgtgtac
     1561 ctggcgacaa acagccttca ggggccgtgg tggatactca gctggccgga gcgggtgccg
     1621 ggggcggacg aggtgctgcc gccggagccg cccgcatacc gggtgctgac gggcgtggtg
     1681 gacggcttcg ggcggacgct ggcctttcac cgggcagcgg agggtgacgt ggcgggcgcg
     1741 gtgacggggg tgatggacgg cgcgggccgc cgttttcacc tggtgctgac cacacaggcg
     1801 cagcgggcgg aagaggcccg taaaccgcac accgcttcgt tatcttcccc tgacagtccc
     1861 tgccctctct cagctccttc cttccccgac acgctgcccg ccggtacaga gtacggtgcc
     1921 gataacggta tccggctgga ggcggtatgg ctgacacacg acccggcata cccggatgaa
     1981 cagccgactg cgccgctggc gcgctacacg tacacggccg gtggtgaact gcgggcggtg
     2041 tatgaccgca gcgggatgca ggtgcgcggg tttacttatg atgcggagca cgccgggcgg
     2101 atggtggcgc accattatgc ggggaggccg gagagc
//
LOCUS       DAZPEP010000308         2135 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZPEP010000308 DAZPEP010000000
VERSION     DAZPEP010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.310"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..503
                     /locus_tag="ACHUT9_004456"
     CDS             <1..503
                     /locus_tag="ACHUT9_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1350745.1"
                     /translation="HNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNN
                     KILKHIKSHLIMLSAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTID
                     GLKEFDKYKTRILDSGINCKKYPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSY
                     REVNRD"
     gene            496..978
                     /gene="yqeJ"
                     /locus_tag="ACHUT9_004457"
     CDS             496..978
                     /gene="yqeJ"
                     /locus_tag="ACHUT9_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFR1350746.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(1012..1092)
                     /locus_tag="ACHUT9_004458"
     CDS             complement(1012..1092)
                     /locus_tag="ACHUT9_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350747.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(1128..1552)
                     /gene="yqeK"
                     /locus_tag="ACHUT9_004459"
                     /pseudo
     CDS             complement(1128..1552)
                     /gene="yqeK"
                     /locus_tag="ACHUT9_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            1982..>2135
                     /locus_tag="ACHUT9_004460"
     CDS             1982..>2135
                     /locus_tag="ACHUT9_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350748.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLG"
BASE COUNT          783 a          352 c          354 g          646 t
ORIGIN      
        1 tccataatga cattaacata caaaatcatg taataaactc gtcaacagac gcacatacac
       61 acaatgcccc acctgccata aaagttaatg cgggatacaa agagagcatt ggtggcgcaa
      121 agaatttcaa taacaaaatc ctcaaacata taaaaagtca tctaattatg ttgagcgcat
      181 ttgtcatagg cgcatattct gcatattggc tatggaataa taatcaaccg aagccatttt
      241 tcaaggatta caagactgtt gcggaaatta atggctgcca ttttaatgta acagaagata
      301 caattgatgg gttgaaagag ttcgataaat ataaaacacg gatactggat tcggggatta
      361 actgtaaaaa atatccgtgg ttatacttcc ctcttgcaaa atcctcaccg gggatgattg
      421 ttatggcatg caataaaaac tataaccaac atgaagtggc agactgttta accctgtctt
      481 accgtgaggt taatcgtgat tgattataaa aaaaacttat tatttatttt agtttttatc
      541 agtgggttta ttctcttcac tgtctattct tacactgcag aaaaaatgat atataacgaa
      601 acatgcactg caaattgggt aatttttaat gaccagggac gagcaaatct taccatcgac
      661 tttatgtata acaaaaaaaa taaaacagga actgttgcgt tgagtggaac ctggcaacaa
      721 ggtaacagag aaagtaaatc aatacggcga aatattgaat acacatggat tgaaaactat
      781 gacacagccc atttaacatc aaagaaagtt aataagtttg agattatgga tcaagttgat
      841 gatgatagac ttgcacaact tattcctgat ttttatgtct ttccagaaaa aagtgtaagc
      901 tataatatac taaagcaagg taagcatgct tttattttga gcattggtaa cagagcaata
      961 atgcattgtg caaggtaaac taaaatagat aacaaaaaag atggaacctc gttaatcata
     1021 aaacaataaa attaaatatt ctcgcagtat atggcagtct aaagcatcaa agttttgatc
     1081 aacatctttc attttagaca tctccttgca atgtaaaata tcatgtatca acaacatctg
     1141 gtgcaaatcc atgagtcgaa ctcgacattc atcttagctc gattattatt ccttcgtaca
     1201 gtcgatgtaa acaatacaga aagaggatta ttaagaacca gtttactcag gaatcaatag
     1261 aggaaaggac taacgtttct ttaaaagaat tgatttcatc atctgttaaa ctaaactcat
     1321 cattgacaga tcgtgagata taactgtttt taactttact ctttacgttg actttattga
     1381 cagaattaac attcacatat cttgaattta atgtccatga tgttgtttgc gtgagaacat
     1441 tctcagcatt aaggaatttt tttaacggaa tccttggttt ctttttcgag tccgaattta
     1501 caatatcatg catatagacc atttcatgaa tctgcgaaaa ctcaatgtcc attgtatacc
     1561 tcacattttt accgtgactc gatgttactg ttcaataatc accttccatc aatactaaaa
     1621 ttaatacccc taatgtgccg ataacaaata tagtcattct acgtaacgtc tccataaggt
     1681 gataaccttg atcaaatgaa tttcgaatat aataaaggaa atcttttgag atgtttatca
     1741 cgaataaagc tatttatgtt accaatatca tttcgagtgc gcacactatc tatctgtttt
     1801 ttcgagatat aaaaaacaac gtgtaagtta tgtaaattgc cgatgctact gagtaacatt
     1861 atggtaaaaa aagtaaaaat gagcgatttt tgatagaata aagcatgaga aaaggtgata
     1921 tttgacatta tcagaagctg cgaattcgga ttttgctcta atcaaatgag agaaatgtaa
     1981 aatgaagcca cgaaatatta ataatagcct accactgcaa ccattagttc ctgatcagga
     2041 gaacaaaaat aagaaaaatg aagagaaatc cgttaatcca gttaaaatca caatggggtc
     2101 tggtttaaat tatattgaac aagaatctct tggag
//
LOCUS       DAZPEP010000309         2093 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZPEP010000309 DAZPEP010000000
VERSION     DAZPEP010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.311"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..123)
                     /locus_tag="ACHUT9_004461"
     CDS             complement(<1..123)
                     /locus_tag="ACHUT9_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFR1350749.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPY"
     gene            384..719
                     /locus_tag="ACHUT9_004462"
     CDS             384..719
                     /locus_tag="ACHUT9_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1350750.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1000..1131)
                     /locus_tag="ACHUT9_004463"
     CDS             complement(1000..1131)
                     /locus_tag="ACHUT9_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFR1350751.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(1631..1707)
                     /locus_tag="ACHUT9_004464"
     tRNA            complement(1631..1707)
                     /locus_tag="ACHUT9_004464"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1671..1673),aa:Arg,seq:tct)
     gene            complement(1808..1883)
                     /locus_tag="ACHUT9_004465"
     tRNA            complement(1808..1883)
                     /locus_tag="ACHUT9_004465"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1848..1850),aa:Ile,seq:cat)
     gene            complement(1986..>2093)
                     /locus_tag="ACHUT9_004466"
     CDS             complement(1986..>2093)
                     /locus_tag="ACHUT9_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1350752.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTRLIFPESVVNSS"
BASE COUNT          677 a          522 c          370 g          524 t
ORIGIN      
        1 ataaggggtt atggccaccg gactaccaaa cggctgccag ccctctttca gtttatgtgt
       61 cagcttttcc gcaagatctg acggcggcgc cgccctgaca acatcatagt gtttaaatgc
      121 catggttctt tccaccatct gaaaaataat tctttaaaat acctgacatg taatacagaa
      181 aaaacacaaa accatacctt aaataaaaac ctgattatca agcagatatg catggataaa
      241 ctacaagacg agatataaac caccctgcat ttaaataaac aataaacaac atcagaaaaa
      301 taattctgct ctatggttta cattcaaaaa tatcatttat acttttcaga acatcaccag
      361 caaggcataa acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat
      421 catgcggaat atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat
      481 cggatgcttt cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca
      541 ataacaaagt gctacctgta accatttaca acgtagtacc attcaataaa acattctgga
      601 attcaatcaa aaatagccag gaatgcccta caaatacaga taacgtattg aatgaatgct
      661 ttaataaccg ttgcactctg caaatatgtc cttatggact aaaacaacaa agtccataag
      721 gagtttactc acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag
      781 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
      841 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
      901 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
      961 tgaagtattt aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt
     1021 tttcagcaac gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccagcac
     1081 tgacattatc cggctgctgc aatgccattc acaaaaacag taagcaatca ctttttaccg
     1141 taacaggtga taatccagat atgtatctac cccagatgag taatccgaag ttcatccata
     1201 ccacaggtcc tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca
     1261 accatgaact catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt
     1321 cccgaaagac gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt
     1381 gctgcattga ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata
     1441 ttcactctac ccacaccata tgaatgataa atatttaatg ctgaaaaaac cattagaccg
     1501 tataacaaac actcaatcaa tacttaacag aacttttatt tttgacaaac ataaaatatt
     1561 ttcaacaata tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga
     1621 agaatgaata tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat
     1681 tgctctgtcc ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc
     1741 gcccccgcac caacaaccct gttatcatgt cgtctgctct tcctgataag ctaatggcgg
     1801 tttgtgatgg tggcccttgc tggatttgaa ccagcgacct ggcgattatg agtcgctcgc
     1861 tctcaccact gagctaaagg gccgggagca gaataataac ggtccgtaat taattccgca
     1921 ataaaaaacc cgctcggcgg cgggttgtag aaactcttct aacgtcaggc ataaaacgcc
     1981 catagttatg acgaatttac cacagattcc ggaaaaatca acctagttac cttttttaac
     2041 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ata
//
LOCUS       DAZPEP010000310         2089 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZPEP010000310 DAZPEP010000000
VERSION     DAZPEP010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.312"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(60..1025)
                     /locus_tag="ACHUT9_004467"
     CDS             complement(60..1025)
                     /locus_tag="ACHUT9_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFR1350753.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1879..>2089)
                     /locus_tag="ACHUT9_004468"
     CDS             complement(1879..>2089)
                     /locus_tag="ACHUT9_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350754.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          577 a          550 c          436 g          526 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gcaggataga cagagcacta gagattgcat gcaatctcgt acctatcacc tcagcctgcc
     1861 gctggctctt ttccagaact aagaatgcgt tcttaaaaat ccaaacttga taccgtttaa
     1921 atctattttc acaaaaagag tttttaccct ctcgtttgtt ttctgaatat ataaaattaa
     1981 ttcatcctca aaaccttcgt gcggatgaaa accaaatgtg ccaaaactta atccatcaat
     2041 aagcaaaata aggtttttaa acctgtcagg aatcaccgct tcacttctt
//
LOCUS       DAZPEP010000311         2028 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZPEP010000311 DAZPEP010000000
VERSION     DAZPEP010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.313"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..271)
                     /locus_tag="ACHUT9_004469"
     CDS             complement(<1..271)
                     /locus_tag="ACHUT9_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1350755.1"
                     /translation="MGLDWVPFSFQKEPMDIAQERSTKKIVLQSCSQLLKTTVLQSIA
                     FNLMANDPCNFAFGSSSESEVKKFKDGKFLPAIETSSVLKPLVTDK"
     gene            complement(315..860)
                     /locus_tag="ACHUT9_004470"
     CDS             complement(315..860)
                     /locus_tag="ACHUT9_004470"
                     /inference="COORDINATES: protein motif:HMM:NF019114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFR1350756.1"
                     /translation="MDISLRSLARQYGYDESTVRTWVEKGMPTDTDSNARSWIVDNVL
                     KPLRDTNTKEQIEQERLKKLSAERQLAELELAEKNGLVVSTEYIEQVLTEYLFQVKTA
                     VRAIPSKTYLELFAQSDAKDLRDVLRTHIDKTLYQLGSMEFELPDDMEVIEDGNKQEE
                     INEDTEESTTDDTTAEDTENE"
     gene            896..1459
                     /locus_tag="ACHUT9_004471"
     CDS             896..1459
                     /locus_tag="ACHUT9_004471"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350757.1"
                     /translation="MSDRNNDFSLLVKLLNGFLKKDEIKKRLYSFVKKETNDWEKWLQ
                     IEFAHYLTSKRKYELHREVTIYLDPIDYPDSKLAKVDLILREKSTPSDDYIFIEFKCT
                     KGVVPLVRGLNADKNKISAIRQCNYKMRNYLGVGFHLFCEPDDIEFMEKYVTEELKGS
                     YAVFKLCECPVRYNCQCEFNEIGVVLF"
     gene            complement(1605..1907)
                     /locus_tag="ACHUT9_004472"
     CDS             complement(1605..1907)
                     /locus_tag="ACHUT9_004472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350758.1"
                     /translation="MEKLILLLLLPLTTSAAIPDFKCWDIHNDSFSTDMSFYVTRYLG
                     KMPPPYGAFHYETAPDEKFYRELVITNEAFDDGYNYGSRYLNKEGTLYDDIKCKRL"
BASE COUNT          615 a          371 c          342 g          700 t
ORIGIN      
        1 tcttatcggt tactaacggc tttagtactg aactggtttc aatagctgga agaaatttac
       61 catccttgaa tttcttcact tctgattcag aactactacc aaaagcaaag ttacagggat
      121 cattagccat taggttaaat gcaattgatt gtagtactgt agttttcaaa agctgggaac
      181 aagattgaag tacaatcttt ttagtacttc gctcttgagc tatatccatt ggttcttttt
      241 ggaaactaaa cggaacccaa tcaaggccca tattcggccc gtccacaaat ttaactacac
      301 cattactaat ccactcactc gttttctgta tcttcggcgg ttgtatcgtc ggtagtactt
      361 tcttcagtat cctcgttaat ttcttcttgt ttgtttccat cctctattac ttccatgtca
      421 tcgggtaact caaattccat tgagcctaat tggtataatg ttttatcaat atgcgttctt
      481 aatacatcgc gtaaatcctt tgcatctgat tgtgcaaata attcaagata agtttttgat
      541 ggtattgcac gcactgctgt tttgacctgg aatagatatt ccgttagtac ctgttctata
      601 tattcagtac tgaccaccag cccattcttt tctgctaatt ctaattctgc taactgccgt
      661 tctgctgaaa gcttcttcaa acgctcttgt tcaatttgtt cttttgtatt agtatcacgc
      721 aacggcttta gtacattatc tacaatccaa cttcttgcgt ttgaatcagt gtctgtcggc
      781 attccctttt ctacccaagt tcgaactgta gattcatcgt atccgtactg tctcgctaat
      841 gaccttaatg agatatccat atttgttcct ttttatttta tttatagggt ttacaatgtc
      901 agaccgtaac aatgattttt ctctgttagt aaaattgctg aacggatttt tgaagaaaga
      961 tgagataaaa aaaagactat attctttcgt gaaaaaagaa actaatgatt gggaaaagtg
     1021 gctgcaaata gaatttgctc attatctaac ttcaaaaagg aagtacgagc tacatcgtga
     1081 agttacgata tacctcgatc caatagatta tcctgatagt aaacttgcta aagtagattt
     1141 gatactgcgt gaaaaatcca caccctctga tgattatatt tttattgagt ttaaatgtac
     1201 taaaggtgta gtaccattag ttaggggctt aaatgctgac aaaaataaaa ttagtgctat
     1261 aagacagtgt aactacaaaa tgcgtaatta tcttggtgtc ggatttcatc ttttttgtga
     1321 accagatgat atcgagttta tggaaaagta cgtaactgaa gaacttaaag gctcttacgc
     1381 cgtgtttaaa ttatgcgagt gtccagtaag atataattgc caatgtgaat ttaatgaaat
     1441 tggtgtggta cttttttaaa tgcggtgcgg gtatgatcat tttgttcaca cacaattgat
     1501 taaacccgct gccgaaaacc ctcgatagta ccgccagtat ggagggaccc attgaatgca
     1561 atggttaata tactttgtaa gctatataca atgaatcatg agtatcaaag ccgcttacat
     1621 ttaatatcat cgtacaaagt tccttctttg tttagatatc tcgaaccgta attatatcca
     1681 tcatcaaatg cttcattggt tattaccagt tccctgtaaa atttctcatc aggtgctgtt
     1741 tcataatgaa atgcaccata aggcggcggc attttaccga gatatcttgt aacgtagaaa
     1801 ctcatgtcag tactaaatga atcgttatga atatcccagc atttaaagtc tggaatagct
     1861 gcacttgttg ttaaaggtaa taataatagc aatatcaatt tttccataag agataccaat
     1921 gcaaatgatt taattgcatg ataacaccta caaagtctca ataccaatta acaagcgata
     1981 tggctgattt tgtttcaatt acgtaaaata atttaaaata tcgttaat
//
LOCUS       DAZPEP010000312         2016 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZPEP010000312 DAZPEP010000000
VERSION     DAZPEP010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.314"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..351
                     /locus_tag="ACHUT9_004473"
     CDS             <1..351
                     /locus_tag="ACHUT9_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HFR1350759.1"
                     /translation="DIKFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNG
                     KYFDSGYIPCSRADESIQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFG
                     QTILRKVTFSNIES"
     gene            388..2016
                     /locus_tag="ACHUT9_004474"
                     /pseudo
     CDS             388..2016
                     /locus_tag="ACHUT9_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
BASE COUNT          635 a          380 c          398 g          603 t
ORIGIN      
        1 gacattaaat ttatagctga tatacagttt aatactgact tatgtggtga cacttatatt
       61 tccagaggtc ccgttttata tgcaattgag cttgagagcg atattttaat caaaaaaaac
      121 ctgctgaacg ggaaatattt tgactcagga tacataccct gtagtagagc tgatgaatct
      181 atccagttct cttatgctga tatggaatct ttttcatatt caacctcacc agagggcaaa
      241 caatacattg aaggctgttt ttatttaaac aagactaaaa tagtcagaaa acttatacca
      301 tttggtcaaa ccattcttcg caaagtaact ttctccaata tagagagtta atattttcga
      361 gtgttataat taattaaagg gatttctatg taccttactg ataatattat caaactgtcg
      421 aataaaatag gtcagcgtgt ttatactgtt gtctctgaaa tgtcgattga agcatggata
      481 acaaaagaac cgcagccttt tgtttatcgc cgtagcggcg cttaccagaa actcaccatt
      541 ggctcagact ggggtcaact gtttgactgt gcctggatgc gagtttatgg taaacgtcca
      601 gcagataaat ttagacacaa actcgtcgct ctcgttgata tcggtggcga aggtcttatt
      661 gtggataaga atggttcacc catctgcggc atcacgaata aagcatcatc ttatggcgta
      721 ccgccagata aaccaggtaa atgggtggtt gacctttctc tcgttagtga aaatgacgag
      781 gttgagtttt ggattgatgc tgcctgcaac gatttatttg gctatgttac taatggaggg
      841 attattactg atgttcatat cgcgacacgt aatcaactac tgaaatccct ttattatgat
      901 attgaggtgc ttttcgactg gattaatgat ggtcagaagt ttgaatccat acatcctaaa
      961 ggtatcaccc cagaacaatt tattgccaaa agaagtgagc gtactgacga aatcataagt
     1021 actctcgaat atattgataa tacgttaatt acctttagca atgaagaaat aattaaatgt
     1081 caaattgcaa tccaatccat aatcaataaa aacaataatc cttcagagtt caggattatg
     1141 gctactggtc atgcccattt agatatagcg tggatgtggc ctttaagaga aggacgcaga
     1201 aaagcaataa gaacatttgc caccgccctt gcaaacattg aaaaatatcc tgattacatt
     1261 tttggcgcta gtcagtacca actttttcac tggataaaaa aagattatcc ctatttcttc
     1321 gaaaagttaa aaaaacaaat cgccgcaggc cgctttgagt tacaaggttg cttctgggtc
     1381 gaatgcgatt taaatctggt aagtggtgaa tcgctgataa ggcaaatcat gcatgggaca
     1441 cgattcacac tgcagaattt ttcaaaaaag ataaattatg tctggcaacc ggatgtcttt
     1501 ggattccccg caacgcttcc acaaattctc aaaaaatcag gcattaacta tattgcatcg
     1561 caaaagctct cacaaaacaa gattaataaa tttaataatt accttttccg ctggcagggc
     1621 ctggatggct cagaaattct gatgcataac tttcctgaag acacttatga tagccgagca
     1681 cgtgctcgct ctttagaata tattgaacaa aactataatg aaaaagagat ctgtccctat
     1741 gcgcttatgg tgtatggtgt atggtgtcgg tgatggcggc gctggccccg gtgaggaaca
     1801 tatagaacga ttaacacgta tacgcaatat tgatggctta cctcatgttg atttcagccg
     1861 tgttgataag ttcttcactt acgccgacgc ctttcgcgaa agcttgccca ttatttcagg
     1921 tgaactttat ttcgaggctc atcaagggtg ctttacatca gagtcagcaa ctaaagctca
     1981 taatcgaaat atggaaaata aattacatga tgctga
//
LOCUS       DAZPEP010000313         2005 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZPEP010000313 DAZPEP010000000
VERSION     DAZPEP010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.315"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..389
                     /locus_tag="ACHUT9_004475"
     CDS             <1..389
                     /locus_tag="ACHUT9_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350760.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUT9_004476"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="ACHUT9_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFR1350761.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUT9_004477"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="ACHUT9_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFR1350762.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="ACHUT9_004478"
     tRNA            complement(1806..1881)
                     /locus_tag="ACHUT9_004478"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="ACHUT9_004479"
     tRNA            complement(1890..1966)
                     /locus_tag="ACHUT9_004479"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAZPEP010000314         1993 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZPEP010000314 DAZPEP010000000
VERSION     DAZPEP010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.316"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..314
                     /locus_tag="ACHUT9_004480"
     CDS             <1..314
                     /locus_tag="ACHUT9_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350763.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            416..1870
                     /gene="amn"
                     /locus_tag="ACHUT9_004481"
     CDS             416..1870
                     /gene="amn"
                     /locus_tag="ACHUT9_004481"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFR1350764.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIDIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          477 a          494 c          475 g          547 t
ORIGIN      
        1 tggcgcttga agcacaatct attttctgga tagttttctt ctccataatg ctggcaaaca
       61 ttgcgcatga catggtggtg tgtgtgcaac aaccgatgtt taccgaaatg tttggtgcca
      121 gttatcgcta tagtggcgct ggagtcggtt atcaggttgc cagtgtggtt ggcggtggat
      181 ttacaccttt tattgccgct gcactcatca cttactttgc cgggaactgg catagcgtcg
      241 ccatttattt gctggctgga tgcctgattt ccgcaatgac cgctttgttg atgaaagaca
      301 gtcaacgcgc ttgatagcct ggcgaagatc atccgatctt cgccttacac ttttgtttca
      361 catttctgtg acatactatc ggatgtgcgg taattgtatg gaacaggaga cacacatgaa
      421 taataagggc tccggtctga ccccagctca ggcactggat aaactcgacg cgctgtatga
      481 gcaatctgta gtcgcattac gcaacgccat tggcaactat attacaagtg gcgaattacc
      541 tgatgaaaac gcccgcaaac aaggtctttt tgtctatcca tcactgaccg taacctggga
      601 cggtagcaca accaatcccc ccaaaacgcg cgcatttggt cgttttactc acgcaggcag
      661 ctacaccacc acgattactc gccctactct ctttcgttcg tatcttaatg aacaacttac
      721 gttgctgtat caggattatg gtgcgcatat ctctgtgcaa ccctcgcagc atgaaatccc
      781 ttatccttat gtcatcgatg gctctgaatt gacacttgat cgctcaatga gcgctgggtt
      841 aactcgctac ttcccgacaa cagaactggc gcaaattggc gatgaaactg cagacggcat
      901 ttatcatcca acggaattct ccccgctatc gcattttgat gcgcgccgcg tcgatttttc
      961 cctcgcacgg ttgcgccatt ataccggtac gccagttgaa cattttcagc cgttcgtctt
     1021 gtttaccaac tacacacgtt atgtggatga attcgttcgt tggggatgca gccagatcct
     1081 cgatcctgat agtccttaca ttgccctttc ttgtgctggc gggaactgga tcaccgccga
     1141 aaccgaagcg ccagaagaag ccatttccga ccttgcatgg aaaaaacacc agatgccagc
     1201 atggaattta attaccgccg atgctcaggg tattactctg gtgaatattg gcgtgggacc
     1261 atcaaatgct aaaaccatct gcgatcatct ggcagtgcta cgcccggatg tctggttgat
     1321 gattggtcac tgtggcggat tacgtgaaag tcaggccatt ggcgattatg tacttgcaca
     1381 cgcttattta cgcgatgacc acgttcttga tgcggttctg ccgcccgata ttcctattcc
     1441 gagcattgct gaagtgcaac gtgcgcttta tgacgccacc aagctggtga gcggcaggcc
     1501 cggtgaggaa gtcaaacagc ggctacgtac tggtactgtg gtaaccacag atgacaggaa
     1561 ctgggaatta cgttactcag cttctgcact tcgttttaac ttaagccggg ccgtagcaat
     1621 tgatatggaa agtgcaacca ttgccgcgca aggatatcgt ttccgcgtgc catacgggac
     1681 actactgtgt gtttcagata aaccattaca tggtgagatt aaacttcccg gtcaggctaa
     1741 ccgtttttat gaaggcgcta tttccgaaca tctgcaaatt gacattcggg cgatcgattt
     1801 gctgcgcgca gaaggcgacc gactgcattc gcgtaaatta cgaaccttta atgagccgcc
     1861 gttccgataa taagttctga accttcttat cagaaataga tgtgaaggag taagtaagac
     1921 acctggcaaa tagcctgcaa taatcgtggg ctgtttgctt ccttgggcgg atacgagttt
     1981 tattatcgtc tta
//
LOCUS       DAZPEP010000315         1855 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZPEP010000315 DAZPEP010000000
VERSION     DAZPEP010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.317"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1129
                     /gene="istA"
                     /locus_tag="ACHUT9_004482"
     CDS             <1..1129
                     /gene="istA"
                     /locus_tag="ACHUT9_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HFR1350765.1"
                     /translation="QGAYIVDIATQIGCSERTVRRYLKYPEPPARKTRHKMVKLKPFM
                     DYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKMRPSKRTVRFETQPGY
                     QLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDAEHTYESLVRAFRYFG
                     GCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRACRPRRARTKGKVERMV
                     KYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKETPEQRFALEQEHLQPL
                     PDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRISLDDELRIYSNEKLV
                     ASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1129..>1855
                     /gene="istB"
                     /locus_tag="ACHUT9_004483"
     CDS             1129..>1855
                     /gene="istB"
                     /locus_tag="ACHUT9_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HFR1350766.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRK"
BASE COUNT          473 a          455 c          535 g          392 t
ORIGIN      
        1 gcagggcgcg tacatcgtcg atattgcgac tcagattggt tgctctgaac ggacggtcag
       61 acgctacctc aaataccctg aaccgccagc cagaaagacc cgccacaaaa tggttaagct
      121 gaaaccgttt atggattaca tcgacatgcg cctggcagag aatgtctgga atagcgaggt
      181 tatcttcgcg gagattaagg caatgggtta tacgggcgga cgttccatgc tgcgttacta
      241 catccagccc aaacgtaaaa tgcgtccgtc aaaaagaaca gttcgcttcg aaactcagcc
      301 tggataccag ctccagcatg actggggcga agttgaggtg gaggttgccg ggcaacggtg
      361 caaagttaac tttgcggtta atacgctggg gttctcccgc agcttccatg tcttcgccgc
      421 accaaaacag gatgctgagc atacctacga atcactggtt cgcgccttcc gctacttcgg
      481 tggttgtgtg aaaacggtgc tggttgataa ccagaaggct gcggtgctga agaataacaa
      541 cgggaaagtc gtgttcaact ccggattcct gttgctggct gaccactata acttcctgcc
      601 acgggcatgc cgtccacgca gggccagaac aaaaggtaag gttgagcgga tggtgaaata
      661 cctcaaggag aacttcttcg tccggtaccg caggttcgac agcttcactc atgtcaatca
      721 acaactggag caatggatag ccgatgtggc tgacaaacgg gagcttcgcc agttcaaaga
      781 aacgccggaa cagcgcttcg cgctggagca ggaacatctg cagccgttac cggatacaga
      841 cttcgatacc agttacttcg atatccgcca tgtgtcctgg gacagctata tcgaggttgg
      901 tggtaatcgt tacagcgttc ccgaagcgct gtgtggtcag ccggtatcga tacgaatatc
      961 gctggatgac gagttgcgga tctacagtaa tgagaaactg gtggcctcac atcgcctctg
     1021 ttcagcatcg tctggctggc agacagtgcc ggagcatcac gccccgctct ggcagcaggt
     1081 cagtcaggtg gaacatcgac cactgagtgc ctatgaggag ctgttgtgat gcatgaactg
     1141 gaagtcctgc tgagtcgcct gaaaatggag catctgagtt atcacgttga aagcctgctg
     1201 gaacaggcag ctaaaaaaga gctgaactac cgggagttcc tgtgcatggc gctacagcag
     1261 gaatggaacg gcaggcatca gcgcggtatg gagtccaggc tgaagcaggc tcgtctgccg
     1321 tgggtcaaaa cgctggagca gttcgacttt accttccagc cgggcatcga ccgtaaggtt
     1381 gtccgggaac tggctggtct ggcgttcgtg gagcgcagcg aaaacgtgat cctgctgggc
     1441 cctcctggtg tcggaaaaac tcatctggcc atagctcttg gcgtgaaagc ggtggatgcg
     1501 ggacatcggg tactgtttat gccactggac agactgatcg cgacactgat gaaagcgaaa
     1561 caggaaaacc ggctggagcg tcagctacag caactgagtt atgcccgggt gttgatcctg
     1621 gatgaaatag gctatctgcc gatgaacaga gaggaagcca gtctgttctt ccggctactg
     1681 aaccgtcgat atgaaaaagc gagcatcata ctgacgtcaa acaaagggtt cgcagactgg
     1741 ggagaaatgt tcggagatca cgtactggca acagcgatcc tggatcggct gctacatcac
     1801 tcaaccacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg taaag
//
LOCUS       DAZPEP010000316         1851 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZPEP010000316 DAZPEP010000000
VERSION     DAZPEP010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.318"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..139)
                     /locus_tag="ACHUT9_004484"
     CDS             complement(<1..139)
                     /locus_tag="ACHUT9_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFR1350767.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RS"
     gene            892..1347
                     /gene="csgB"
                     /locus_tag="ACHUT9_004485"
     CDS             892..1347
                     /gene="csgB"
                     /locus_tag="ACHUT9_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFR1350768.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            1388..1840
                     /gene="csgA"
                     /locus_tag="ACHUT9_004486"
     CDS             1388..1840
                     /gene="csgA"
                     /locus_tag="ACHUT9_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFR1350769.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          581 a          341 c          406 g          523 t
ORIGIN      
        1 gagaacgttg aatattatgt aattttcctg taattgccag cgattgttta aggtgctgca
       61 agagagccgt cgcctgcaaa gaaggtttag tgatcaacaa taatgtatga ccatgaatac
      121 tatggacttc attaaacatg atgaaacccc gcttttttta ttgatcgcac acctgacagc
      181 tgcctctaaa atagaagcac cagaagtact gacagatgtt gcactgctgt gtgtagtaat
      241 aaatcagccc taaatgggta aaatataaaa ctaatggatt acatctgatt tcaatctagc
      301 cattacaaat cttaaatcaa gtgttaaaca tgtaactaaa tgcaactcgt tatattaaaa
      361 tgttaatctt aaggttttat taagtttaga aatgatagaa aagttgtaca tttggttttt
      421 attgcacaat tttaaaaaat catacaaatg gtgataactt actaataatg catataaaaa
      481 atatttcggt gtagtccttt cgtcatgtaa aaagttgttg ttttttctcc acacctccgt
      541 ggacaaattt ttactccaaa aagacgaggt ttgtcacggc ttgtgcgcaa gacatatcgc
      601 agcaatcagc gatgggcaag aagaatgact gtctggtgat ttttgatagc ggaaacggag
      661 atttaaaaga aaacaaaata ttttttgcgt agataacagc gtatttacgt ggcttttaat
      721 actttggtat gaacaaaaaa agaaaaatac aacgcgcggg tgagttatta aaaatatttc
      781 cgcagacata ctttccatcg taacgcagcg ttaacaaaat acaggttgcg ttaacaacca
      841 agttgaaatg atttaatttc ttaaatgtac gaccaggtcc agggtgacaa catgaaaaac
      901 aaattgttat ttatgatgtt aacaatactg ggtgcgcctg ggattgcagc cgcagcaggt
      961 tatgatttag ctaattcaga atataacttc gcggtaaatg aattgagtaa gtcttcattt
     1021 aatcaggcag ccataattgg tcaagctggg actaataata gtactcagtt acggcaggga
     1081 ggctcaaaac ttttggcggt tgttgcgcaa gaaggtagta gcaaccgggc aaagattgac
     1141 cagacaggag attataacct tgcatatatt gatcaggcgg gcagtgccaa cgatgccagt
     1201 atttcgcaag gtgcttatgg taatactgcg atgattatcc agaaaggttc tggtaataaa
     1261 gcaaatatta cacagtatgg tactcaaaaa acggcaattg tagtgcagag acagtcgcaa
     1321 atggctattc gcgtgacaca acgttaattt ccattcgact tttaaatcaa tccgatgggg
     1381 gttttacatg aaacttttaa aagtagcagc aattgcagca atcgtattct ccggtagcgc
     1441 tctggcaggt gttgttcctc agtacggcgg cggtaaccat ggtggtggcg gtaataatag
     1501 cggcccaaat tctgagctga acatttacca gtacggtggc ggtaactctg cacttgctct
     1561 gcaaactgat gcccgtaact ctgacttgac tattacccag catggcggcg gtaatggtgc
     1621 agatgttggt cagggctcgg atgacagctc aatcgatctg acccaacgtg gcttcggtaa
     1681 cagcgctact cttgatcagt ggaacggcaa aaattctgaa atgacggtta aacagttcgg
     1741 tggtggcaac ggtgctgcag ttgaccagac tgcatctaac tcctccgtca acgtgactca
     1801 ggttggcttt ggtaacaacg cgaccgctca tcagtactaa tacatcattt g
//
LOCUS       DAZPEP010000317         1845 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZPEP010000317 DAZPEP010000000
VERSION     DAZPEP010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.319"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1456)
                     /locus_tag="ACHUT9_004487"
     CDS             complement(<1..1456)
                     /locus_tag="ACHUT9_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFR1350770.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFT"
     gene            complement(1545..>1845)
                     /locus_tag="ACHUT9_004488"
     CDS             complement(1545..>1845)
                     /locus_tag="ACHUT9_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350771.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
BASE COUNT          578 a          363 c          332 g          572 t
ORIGIN      
        1 tagtaaaagt aacaccatta ataatcatca tttttgccca gccggggtaa cggaaactga
       61 gagcaaaacg aacagatgac tcaggctcaa tgagacattt tacgcttaaa tcatgagggt
      121 attcagttaa ttgtgtaatt ttaattgcaa cattattaaa tgtgccataa aactcacacg
      181 ggccataaaa taatgcagta aaaccattgt tattttcttg aaacatattt tcgacaaaag
      241 ccggtatggt tcttgctgta ttcggtacgc agcagacagc agcctcctca tgagtagggg
      301 agtatttata tctggtatta aactgattac tgtcaggatg tttcctttca tttgcttcat
      361 aacaattatc agtcttacag tacattatcg cgtgaagtat gggatgctgc atgccaaaac
      421 tagcattata gaatagccac tcagcatcat cggccagacc aatattagtg gtcagtttta
      481 ttaacataac ataagtgcta aatagttcat gaacagaaca atattcatag cctgtataag
      541 tggcattagc tgtgcgtcct gtaatccact catcaccaat gggagcgcca gatggtgtaa
      601 gataatattt caactgggat aaaaaaatat ctaatgcagt ttgatactgc ggccgttggc
      661 tcgctgcgat ggctaatgcc ctaatatgct catacgtatg tacgccatga cctttaagaa
      721 ggtaaccagg gtctatgaca tttttatatt gcagatcttg ctccgacacg catcctgcgg
      781 aataatcttc atacagccac aagcaatatg agagatattc aatgttattg gttatttcat
      841 aaaggcggta gaaaacatct gttattgtta atccgtggct atgtccagca aaaccctgtt
      901 ccacaacgaa aggatgtgac gtttctttag ggtagccagc cataatgcat tgtgcggctt
      961 gggtaagaga agttaataaa ctctcattac gggtggactc ataacatcca agcacaaaac
     1021 gcaaggcagt agcttgtgcc cagaactctc cgttttctgc tttacagtta aattttaatg
     1081 atgaatcata aattccaatg tagccatcat catcttgcgt atcgataagt tccaaacata
     1141 aatcgctcgc tttttcctgc cattctttat tatctaacaa gtaggcactg cggcaatatc
     1201 catccttcca attcgattga gtttcactat cccaccaata atattgcatc atgttatcat
     1261 cgggattatt ctgtttttta ataacgccat cttcagcaga tacagtgcgt ggagttatac
     1321 gatctcttcc atatatttta tgctccgtaa taagatgggg aactaattta tccagttctc
     1381 ctatatgacc gttcatatac cattccatct tattctttaa ccaaccagtg ggctttatat
     1441 cgttaaaaaa aatcataaat acacctttgc ataaaaaagt ttagacgtta taaaagacaa
     1501 atagattgtg atcgcctaca gacgatcaca atgaaaggaa aatattaacg gtttttgcta
     1561 tcactcaaat gtttttcatc ctgcaccata tcattgccag attcatgcaa agtatttatt
     1621 aatgcattgg catctggata ttgttgctga tacttggtta gatatatatc actatggata
     1681 tgttcggcaa ttatattgat aagcaagcga ttattcgagt ataaccccag tgattcagct
     1741 cgttgtgaaa aaaccattac tgtcttcata tcaacaacaa gcataaaacg gtgagaaggg
     1801 taatattctt cttcatggtt aaagcggtaa tacagttcta cacgt
//
LOCUS       DAZPEP010000318         1734 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZPEP010000318 DAZPEP010000000
VERSION     DAZPEP010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.320"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..493
                     /locus_tag="ACHUT9_004489"
     CDS             <1..493
                     /locus_tag="ACHUT9_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFR1350772.1"
                     /translation="ADPDYVIWAKEVQGINRAWTFRHWKGTGTVGVMVATNDPERPAP
                     DESVINAVRDHILPLAPVAGSGLYVFGATEKVIPMTIALSKDTPQIRTAIKAELNALM
                     LRDGVPEGRMYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQAYDPA
                     RSK"
     gene            493..1083
                     /locus_tag="ACHUT9_004490"
     CDS             493..1083
                     /locus_tag="ACHUT9_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFR1350773.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1070..>1734
                     /locus_tag="ACHUT9_004491"
     CDS             1070..>1734
                     /locus_tag="ACHUT9_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350774.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTG"
BASE COUNT          433 a          381 c          511 g          409 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaacggt tggcgtgatg gtggcgacaa acgatccgga
      121 acgcccggcc ccggatgaaa gcgtgattaa cgcagtcagg gaccacatcc ttcctctggc
      181 acctgttgcc ggaagcggat tgtatgtatt cggtgccaca gaaaaagtca tcccgatgac
      241 gattgcatta tcgaaagaca caccgcagat caggactgca ataaaagcag agctgaatgc
      301 actgatgtta cgggatggtg tgccagaggg gcgtatgtat ctgtccagaa tcagtgaggc
      361 catcagtttg tctgctggtg aagtggccca ccggttaatc gtcccttcat cggatattga
      421 cctgggggag accgagcttc cggtgctcgg cgagatcacc tggcaggctt atgacccggc
      481 aaggagtaaa taatggattc gttacaggat gattatacaa agctgctgta cggactgatg
      541 ccgccggggc ctgcatggtc agataccgac ggtgtacttg acggtctggc accatcgctt
      601 gtgcgtgttc accagcgggc tgatgaactg gtgattgaaa tcgatcccgg tcagtccaca
      661 gagctgattg aacgttatga agaattgtat ggtttacctg attcctgttc ccctgtcgga
      721 acccagacat tacgccagcg tcagcaacgt cttgaagcaa aagccaatgt ggctggtggc
      781 ataaatgagc agttcttcct ggatcagctt gaggcgctgg gatataccgg cgtaacgatc
      841 gaacagttcc agcacttgga tgcaagcccc gatccggaat ggggagatcg ctggcgttat
      901 ttctggcgtg tgacgttgcc gatggatgcc ggtgctcagt ggcagacatg cacggacgcc
      961 tgcaacacac cgatccggac gtggggggat acggttgcgg agtgcgtgat taataaatta
     1021 tgtccgtcac acaccgtcgt tttattttcc tatccagatg aggatgaaga tgcacaggat
     1081 tgatacgctg actgcggtaa aagataagtt tggtccgggt aagaacggat ttactgacgg
     1141 aaatcttcgc acaggacgtc ttgctacctg gctgaacagt gccatgtgga atgccattca
     1201 ggaggaaatc tgtggtgtca ttgagaaggc cgggatagaa ctgaataaag aagaacacga
     1261 ccagctatat aaggctatat tattgctggt gggcggtgca attaacgaag aggcattgct
     1321 gataaaaaat aacctttcgg atgtggaaga cagggatgag gctgttgaaa acctcggatt
     1381 aaaacccacg gtggataagg caaaaaatgc cgttcagcgt gatggcgaca ccatgaccgg
     1441 agagctgaaa atccgtggtg ttaatgcgct gaggattttc aacgaagctt ttggcctgat
     1501 ttttcgtcgt tcggaagagt gcctgcacct tattcccacc agtgaaggtc agggggaaaa
     1561 tggcgatatt ggtcccctgc gcccgttcac cattaatctg cggacgggtg aaatatccat
     1621 gtcgcataaa gtgtctgttg gcggtggttc gcaggtcaat ggtgcgctgg gtatcggcgt
     1681 tcagaacgcc ctggggggga attcaattgt tcttggtgat aatgacaccg gatt
//
LOCUS       DAZPEP010000319         1730 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZPEP010000319 DAZPEP010000000
VERSION     DAZPEP010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.321"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(80..1030)
                     /gene="corA"
                     /locus_tag="ACHUT9_004492"
     CDS             complement(80..1030)
                     /gene="corA"
                     /locus_tag="ACHUT9_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFR1350775.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1182..1238)
                     /gene="ysgD"
                     /locus_tag="ACHUT9_004493"
     CDS             complement(1182..1238)
                     /gene="ysgD"
                     /locus_tag="ACHUT9_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFR1350776.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            1400..>1730
                     /locus_tag="ACHUT9_004494"
     CDS             1400..>1730
                     /locus_tag="ACHUT9_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350777.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          456 a          439 c          429 g          406 t
ORIGIN      
        1 ttttcaatcc gcatgacttt aaataagaca atttttagaa ataagccgga taaaaaaata
       61 gctttatccg gcactcatat tacagccagt tcttgcgctt aaagtacaga tacggtgcca
      121 ggcccgcgag gatcataaag ataatcgcgc ctgggtagcc gaaactccac ttcagttctg
      181 gcataaactc aaagttcatc ccatagctgg aagcaacgag cgtcggcggc aggaatacca
      241 cggataccac cgagaagatt ttgatgatgc ggttctgctc gatgttgata aaacccatcg
      301 ccgcctgcat caggaagttc accttctgga acagggattc gttatgcggc agcagggatt
      361 cgatatctcg caggatttca cgcgcctgct ccagttgccc acccggtaaa cgcgctttac
      421 gcaccaggaa gttgagcgcg cgctgggtat ccatcagaca caggcgaact ttccagccga
      481 tatcttccag ttccgccaga gtggagagcg cctcgtcgta ctcatcgccc tgatgccctt
      541 ccataatcac ccggctcaac tgctccaggt cgctatagat attttcaatt tcatctgcca
      601 actgttcgat tttggtttcg aacagatcca gcagcagctc gtaggcgtta ccgtctacca
      661 tcgactggct acgggcacgc atacgataca gacgaaaagc gggcagttca cgctcacgca
      721 gagtaaacag acgaccatca cggatggtaa atgccacagt ggagttaccg gcgtgatctt
      781 ccgcatcttc aaagaagaag aaggagtgaa tatgcaggcc gtcgtcgtct tcaaagaaac
      841 gtgccgatgc ttcgatgtct tccagttccg ggcgggttgc caggctctgg ccaagttcag
      901 attgtacgcg cagtcgctcg tcgtcgtccg gttcgacaag atcaatccat actgcattta
      961 caaggggttg tgactcttcg acttccagcc gggtcagtcg gttattttcc agttgaaatg
     1021 cgctcagcat gaccgggact cccaatgctt aaaatatcgg acagttcggt gggcacacag
     1081 aaacaaattg ggtttcagac cattaaacag cctgactcag cgcgacggga aaaattgagg
     1141 tcgctgacaa ccgctaaggc tatcagcaaa aagggatagc cttaggagtt gatcctggat
     1201 gacaggatag tgagccagta tctactgggt gtgtccaagg cgaatgtcct cttagagtga
     1261 tcgtgcgcgc atgttacgcc agcaaaattt tgccgtcaac acgcaacaga acaccacaga
     1321 acaatagttg aacttcgcgc tttttggtac tcgtcaaggt cgctaacagg ataaaaaaat
     1381 ccaacatcca ggacctataa tggcgcatcg gctactcatt ggtaaaggaa tgatcacctt
     1441 gaatctcaaa cggatttttc tcgccctcac cttacttccc ttgtttgctg ttgccgctga
     1501 tgattgcgca ctctcagatc cgatgctgac cgtacaggcg tataccgtta atcctcaaac
     1561 agagcgggtg aaaatgtact ggcaaaaagc caatggcgaa gcgtggggaa ctttacatgc
     1621 tctgctggtg gatatgaata gtcagggtca ggtgcagatg gcgatgaacg gcggcatcta
     1681 tgatgaaagc tatgcgccgc tcggtttgta catcgaaaac ggtcagcaga
//
LOCUS       DAZPEP010000320         1728 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZPEP010000320 DAZPEP010000000
VERSION     DAZPEP010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.322"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..356)
                     /locus_tag="ACHUT9_004495"
     CDS             complement(<1..356)
                     /locus_tag="ACHUT9_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFR1350778.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLA"
     gene            complement(356..850)
                     /locus_tag="ACHUT9_004496"
     CDS             complement(356..850)
                     /locus_tag="ACHUT9_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFR1350779.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            1208..1384
                     /locus_tag="ACHUT9_004497"
     CDS             1208..1384
                     /locus_tag="ACHUT9_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350780.1"
                     /translation="MRDKPANHKRGKNPVCIVFDYSRTLAQKEFPVGLRSWLIREYGD
                     DTAHDVSGLNTFIR"
     gene            complement(1526..1678)
                     /locus_tag="ACHUT9_004498"
     CDS             complement(1526..1678)
                     /locus_tag="ACHUT9_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350781.1"
                     /translation="MPSMIAIILLIILHIWLCRQGGAHFWSESRLNISLLMLDIEHFA
                     RGKGLR"
     gene            complement(1666..>1728)
                     /locus_tag="ACHUT9_004499"
     CDS             complement(1666..>1728)
                     /locus_tag="ACHUT9_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFR1350782.1"
                     /translation="IRDQTALRTLQEYIRTQCLR"
BASE COUNT          441 a          476 c          399 g          412 t
ORIGIN      
        1 ctggcgagtc gttttttgga gtgttcgcgg gctttttcct ccgtcatctg aataatcagc
       61 atatcagcag gatcgcaaat cacgttgtaa atcacccagc cgtcaatcag gccgatagtc
      121 ttgccggttc gtgccgggcc aacaaatatc actgcgtcgt attcacgcga ggccaggcag
      181 ttcatcggct caataacata cggtgccacc agcggatccc acgggactga gttccctgcc
      241 cccatgggca cccgcatata ctgagcaacg gcatcagcaa cccgcattcg tctcggtgcg
      301 cgaaggatat aacctgaatc ggttcgtgct gcctttgcgg tttcctgatt cagcattact
      361 cctcctgctg taattcctcc tcatcatccg cacctgcttc agtcacccgc agggctatct
      421 gatcgcgcag atcatcaata atggactgaa cacggctcac agcggcaggc tgcagaccgc
      481 agtcacgttc aagaatatcc ggtaatgtct ccagcacctg cacgaccgct tttgcccaga
      541 tggcaaactc ccgtctgaca tcactggccg gaatgagttg tgccgtttcc tgttcgaact
      601 taagacgctc acgttcagac tgataccagg ctttgcgctc atgcgcgtcc atttcgcctt
      661 ctgcaaccgg cggtggtaat gccagaaatg ccgacacaat atcaaccacc cgataaagct
      721 tgaggttgct ttcatgcccc cctgcaacgg gtagattttg cagccttgcc gcagcagtct
      781 ggcgatgtac acctgacagt gccgccagtt gactgatatt cagcgtcaga ttttttaact
      841 ctcgatccat acccgctcca gaatgtttta aacatgcatc ttgcgaacaa ctttaggcaa
      901 acggtgttag tgatgaacaa aaaacaatca aaatcgacac cataaaaata aaaccactgt
      961 aatatcaatc cattacagta gtggtgatga cgaatgaaat ttcaaaaact agcctttttc
     1021 cgcgccgctc ccgccccgtg gcaggccacc ccaccgggag gacccgtcag cctgacagcc
     1081 atgacgaacg tctgatacag cgccctgcat gaatgacatc gggataatcc agaaaggaat
     1141 agcatcgtac ccacaagaat cagtgtaagt gtcctgtttc ttccaccccc gcacaggact
     1201 ggcgagcatg agggacaaac ccgcgaacca taaacgcggt aaaaacccgg tgtgcatcgt
     1261 ttttgattat tcccgcacac tcgcgcagaa ggagttcccc gtcgggctac ggtcatggtt
     1321 aatacgggaa tacggcgacg atacagcgca tgatgtgtca ggcttgaata cctttatccg
     1381 ttaaaaggga tatcagttaa gttatcccgt gtagggtata agccattatc aaagccactc
     1441 tgtaggaagt ggcttttgta atggcaataa aaagccccgc gaatgcgagg ctaaatcctg
     1501 gtatttgtaa tgactggctc ttatctcaac gcagcccctt accgcgcgca aaatgctcaa
     1561 tatcaagcat cagcaatgag atgtttaatc tggattcact ccagaagtga gcaccaccct
     1621 gtctacagag ccagatgtga aggatgatga gtaaaattat cgctatcatc gaaggcattg
     1681 cgtcctgatg tattcctgaa gcgttctcag tgctgtttgg tcgcggat
//
LOCUS       DAZPEP010000321         1710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZPEP010000321 DAZPEP010000000
VERSION     DAZPEP010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.323"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..711)
                     /locus_tag="ACHUT9_004500"
     CDS             complement(<1..711)
                     /locus_tag="ACHUT9_004500"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1350783.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIACLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQS"
     gene            complement(692..>1710)
                     /locus_tag="ACHUT9_004501"
     CDS             complement(692..>1710)
                     /locus_tag="ACHUT9_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1350784.1"
                     /translation="NDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEP
                     VEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILG
                     ANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFE
                     QSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQMFL
                     CWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAG
                     LSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSD
                     SRAA"
BASE COUNT          333 a          556 c          458 g          363 t
ORIGIN      
        1 ggactgcaat gtttcccgga catcatccgg tagatggctg taggggttgc catccacctt
       61 atggctgccg ctgtaaatca gcgtgatttc cacgccctgt ttctccagtg cagcgccgta
      121 attactgtga gccatcatga cgccgatgga gcctgtccgg gcggtctgcg tgaccagacg
      181 ccgggaggcg gcactggcaa gcagctgacc tgcgctgcag ttcatgtcat tggccagcgc
      241 ccataccggc tttatgtcac gcacacgggc gatgatgtca gcgcagtcaa atgctcccgc
      301 caccattccg cccggtgtgt ccatatcgag cagaatgccg tccaccatcg ggtcgctggc
      361 agcctgttgc agacaggcga taatgccgtt gtaaccggtc atccccgagt acggctgcag
      421 cgcccgcgtc cggctgacca gcgtgccgga caccggcagc acggcgatgc cgttcattac
      481 ctgataactg cgggcctgtc gtggtccgtc atcatcaccg gataacgcca gcgccgcggg
      541 tgcctctccg gcagtcaggc tgtcgccgga tactgcatcc gtcaggcagc tgatcccaag
      601 ctggcctgca agcgcacaaa agaaaacccg cgcataggcg ggttcaagca tcagcggctc
      661 attaaaagcc atgctggcaa tatgcgggag attacgcagc tctgctgtca ctcttctcct
      721 cctctgttga ttgtcgcagc ccggattcaa atgccgcagc cgcccaggcg ggcggtttaa
      781 gaccagctgc gcggcgctcc atcgtttcac ggacctgctg ggcaaaaatt tcctgatagt
      841 cgtcaccgcg tttcgcgcac tctttctcgt aggtgctcag tccggcttct atcagcatca
      901 ccgcttcctg aacttctttc agaccatcga tggccatacg accggagcct atccagtcgc
      961 agttccccca ggcactgcgg gcttcctgaa agctgaagcg cgcttttgaa ggtaacgtca
     1021 ccacgcggcg aacgatggcc tcttccagcc agcacagaaa catctggctc gcctgacggg
     1081 atgcgacgaa ttttcgccgc cccataaagt acgcccacga ctcgttcgca ctggcccgtg
     1141 ccgtggagta gctcatctgg gcgtaattcc gggaaagttg ctcatacgag acacccagcc
     1201 cggcagcgat ataccgcaac agtgactgct caaacacgga gtagccgtta tccgtgtcct
     1261 gagccgtctg caggttcagt gagtcccccg gcatcaggtg cggcactttt gcgcctccca
     1321 gacggaccgg tgctgcggcg taatacgcgg caatttcacc aatccagccc gtcagccttt
     1381 cccgctgctc cttactgttc gcgcccagaa taaaatccat cgctgactgc gtatccagct
     1441 cactttcaat ggtggcggca tacatcgcct tcacaatggc gctctgcagc tgcgtgttct
     1501 gcagcgtgtc gagcatcttc atctgctcca ttacgctgta aaacacattt gcaccgcggg
     1561 tctgcccgtc ctccacgggt tcaaaaacgt gaatgaacga ggcgcgcccg ccgggtaact
     1621 cacggggtat ccatgtccat ttctgcggca tccagccagg atagccgtcc tcgctgacgt
     1681 aatatcccag cgccgcaccg ctgtcattaa
//
LOCUS       DAZPEP010000322         1710 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZPEP010000322 DAZPEP010000000
VERSION     DAZPEP010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.324"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..711)
                     /locus_tag="ACHUT9_004502"
     CDS             complement(<1..711)
                     /locus_tag="ACHUT9_004502"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFR1350785.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPATLALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQS"
     gene            complement(692..>1710)
                     /locus_tag="ACHUT9_004503"
     CDS             complement(692..>1710)
                     /locus_tag="ACHUT9_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFR1350786.1"
                     /translation="NDSGAALGYYVSEDGYPGWMPQKWTWIPRELPGGRASFIHVFEP
                     VEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKAMYAATIESELDTQSAMDFILG
                     ANSKEQRDKLTGWIGEIAAYYAAAPVRLGGAKVPHLMPGDSLNLQTAQDTDNGYSVFE
                     QSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANESWAHFMGRRKFVASRQASQMFL
                     CWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLIEAG
                     LSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPPAWAAAAFESGLRQSTEEEKSD
                     SRAA"
BASE COUNT          331 a          555 c          465 g          359 t
ORIGIN      
        1 ggactgcagt gtttcccgga catcatccgg tagatggctg taggggttgc catccacctt
       61 atggctgccg ctgtaaatca gcgtgatttc cacgccctgt ttctccagcg cagcaccgta
      121 attactgtga gccatcatga cgccgatgga gcctgtccgg gcggtctgcg tgaccagacg
      181 ccgggaggcg gcgctggcaa gcagctgacc tgcactgcag ttcatgtcgt tggccagcgc
      241 ccataccggc tttatgtcac gcacacgggc gatgatgtca gcacagtcaa atgctcccgc
      301 caccatcccg cccggtgtgt ccatatcgag cagaatgccg tccaccatcg ggtcgctggc
      361 agcctgttgc agacgggcga taatgccgtt gtaaccggtc atccccgaat acggctgcag
      421 cgcccgtgtc cggctgacca gcgtgccgga aaccggcagc acggcgatgc cgttcatgac
      481 ctgataactg cgggcctgtc gtggtccgtc atcatcaacg gataacgcca gcgtcgcggg
      541 tgcctctccg gcagtcaggc tgtcgccgga taccgcatcc gtcaggcggc tgatcccaag
      601 ctggcctgca agcgcacaaa agaaaacccg cgcataggcg ggttcaagca tcagcggctc
      661 attaaaggcc atactggcaa tatgcgggag attacgcagc tctgctgtca ctcttctcct
      721 cctctgttga ttgtcgcagc ccggattcaa atgccgcagc cgcccaggcg ggcggtttaa
      781 gaccggctgc gcggcgctcc atcgtttcac ggacctgctg ggcaaaaatt tcctgatagt
      841 cgtcaccgcg tttcgcgcac tctttctcgt aggtgctcag tccggcttct atcagcatca
      901 ccgcttcctg tacttctttc agaccatcga tggccatacg accggagcct atccagtcgc
      961 agttccccca ggcactgcgg gcttcctgaa agctgaagcg cgcttttgaa ggtaacgtca
     1021 ccacgcggcg aacgatggcc tcttccagcc agcacagaaa catctggctc gcctgacggg
     1081 atgcgacgaa ttttcgccgc cccataaagt gcgcccacga ctcgttcgcg ctggcccgtg
     1141 ccgtggagta gctcatctgg gcgtaattcc gggaaagctg ctcatacgag acacccagcc
     1201 cggctgcgat ataccgcagc agcgactgct caaacacgga gtagccgtta tccgtgtcct
     1261 gagccgtctg caggttcagt gagtcacccg gcatcaggtg cggcactttt gcgcctccca
     1321 gacggaccgg tgctgcggcg taatacgcgg caatttcacc aatccagccg gtcagcttgt
     1381 cccgctgctc cttactgttc gcgcccagaa taaaatccat cgctgactgc gtatccagct
     1441 cactctcaat ggtggcggca tacatcgcct tcacaatggc actctgcagc tgcgtgttct
     1501 gcagcgtgtc gagcatcttc atctgctcca tcacgctgta aaacacattt gcaccgcggg
     1561 tctgcccgtc ctccacgggt tcaaagacgt gaatgaacga ggcacgaccg ccgggtaact
     1621 cacggggtat ccatgtccat ttctgcggca tccagccagg atagccgtcc tcgctgacgt
     1681 aatatcccag cgccgcaccg ctgtcattaa
//
LOCUS       DAZPEP010000323         1707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZPEP010000323 DAZPEP010000000
VERSION     DAZPEP010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.325"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>1707
                     /locus_tag="ACHUT9_004504"
     CDS             <1..>1707
                     /locus_tag="ACHUT9_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015690144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350787.1"
                     /translation="AGVEKHIEQIYIDNEPVLAVPITAEGVVPKESIAAKYQNYLQLE
                     VRFGGDYTSTKTLAAKYAGSKWTNKFLGKGIVSISTVILKTQDSLENNILVNDQFALT
                     VEMKGQVIYDFVDGTYKASSNPPSIIYDYLTNSIYGMGIDPSLINTDTFAETAAYCDA
                     FEYYANGAISYQKTYKDNIENICQSFGGIMYVHAGQICITTDRKSVSVASFDERNMVG
                     AVQVSTSGGTDYFNVVDCKFTNPESMYTTDVVRIPSDITTDEAVQHDGQVIALSRDYS
                     WSYDQEVIAKMANVDVLKAKYALRTISFTTSEGWDLEVWDAINVSNEELAISGKFKVL
                     NKDISTDQENVGYVTITAVEAPDAMYDGIDPGIWSPGGVINFPELQVLPPTNLQAVRK
                     GNTTSGSIVDLTWDASPDPYLRGYYVYYRLNGSSTWTYAGQTGVQKLDYELFGLSDTA
                     SYDFSVEAYNNLGLVSKKLSLTGIVPTFNFALPAVTRVVLTNKTESAYVTDSPDFNIA
                     WDSQKDIRVNNRSFSEYFKYYVIKIYDGTKLVDTFYTQSNAFNFTLEMNRLKVRKPTI
                     GI"
BASE COUNT          527 a          323 c          361 g          496 t
ORIGIN      
        1 atgctggtgt agaaaagcat attgagcaaa tctatattga taatgagcca gtacttgcag
       61 taccgattac agcagagggt gtagtaccta aagaaagtat tgcagcgaag taccagaact
      121 atttgcaatt agaagtacga tttggtggtg actatacgag tactaaaact cttgctgcaa
      181 aatatgctgg ttcgaaatgg acaaataaat ttcttggtaa gggtattgtt agtatcagta
      241 ccgttatctt aaaaactcaa gattcattag aaaataatat tcttgttaat gatcaattcg
      301 ctttaactgt agaaatgaaa gggcaggtga tttatgattt tgttgatggt acttataaag
      361 caagtagtaa ccctccttca attatctatg attacttaac aaattcaatc tatggtatgg
      421 gtattgatcc atcattgatt aataccgata cgtttgcaga aaccgcagca tattgtgatg
      481 cttttgaata ttatgctaat ggtgctatca gttatcaaaa aacctataaa gataatattg
      541 aaaatatctg tcagtcattt ggcggcatca tgtatgtaca tgccggacaa atctgtatta
      601 ccacagaccg caaatcggta tccgtagcct catttgatga gcgtaacatg gtaggggcgg
      661 tacaggtttc tacttctggc ggtactgact acttcaacgt agtcgattgc aagtttacta
      721 accctgaatc gatgtacacc actgacgtag tacgtatccc ttccgatatc actacggatg
      781 aagcagtaca gcatgatgga caggtcatag cattatcacg cgattactca tggtcatatg
      841 atcaagaggt gattgcaaaa atggctaacg ttgatgtact caaggctaag tatgccctgc
      901 gtactatcag cttcaccaca agtgaaggtt gggatctcga agtatgggat gcgatcaatg
      961 tcagcaatga agaactcgct atctcaggca aatttaaagt actcaataaa gatatcagca
     1021 ccgatcaaga gaacgttggc tatgtaacca tcacggcagt agaagcacct gatgcaatgt
     1081 atgatggtat tgatccgggt atctggtcgc ctggtggggt aattaacttc cctgaactac
     1141 aggtactacc gccaacgaac ctacaggcag tacgtaaggg gaatactact tctggttcaa
     1201 tcgtggatct tacctgggat gcttcaccag atccgtatct gcgtgggtac tatgtttact
     1261 accgtcttaa cggttccagt acctggacgt atgcggggca gactggcgta cagaagcttg
     1321 attatgaatt atttggattg tctgatactg caagctacga cttttcggta gaggcttaca
     1381 acaacttagg gctggtatcc aagaagcttt ccctaactgg cattgttcca acattcaact
     1441 ttgcactacc tgcggtaacg cgtgtagtac tgacaaataa aacagaatca gcgtatgtaa
     1501 ctgactcacc agatttcaat attgcctggg attcacaaaa agatatcaga gtgaataacc
     1561 gatcatttag tgagtacttc aagtattacg ttattaaaat ctatgacggt actaaattag
     1621 tagatacatt ctatacgcaa agtaatgcct ttaatttcac tcttgaaatg aaccgtctga
     1681 aagtacgtaa gcctaccatt ggtatta
//
LOCUS       DAZPEP010000324         1705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZPEP010000324 DAZPEP010000000
VERSION     DAZPEP010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.326"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1138
                     /locus_tag="ACHUT9_004505"
     CDS             <1..1138
                     /locus_tag="ACHUT9_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1350788.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFAHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            1150..1599
                     /locus_tag="ACHUT9_004506"
     CDS             1150..1599
                     /locus_tag="ACHUT9_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFR1350789.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEIRDLYAGKIIDLLQMKYFRKEKYNNIRWGGSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIKKEIPLLSQKIGGL"
BASE COUNT          512 a          281 c          490 g          422 t
ORIGIN      
        1 gacatggtac gggtgggacg gtgacaggct gacgacgata cagaccggca ccacgcgtat
       61 ccagacggta taccagccgg ggagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggtgt
      181 gacgctaccg gcggagctgt cggtgatgct ggggaggctg gagcgggaac tgcgcgcggg
      241 agcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg cggagcagat
      301 ggccgcgcag ctggaggcgg aatacatccc ggagaggaaa cttcatcttt accactgcga
      361 ccaccgggga ctgccgctgg cgctcatcag cccggaaggg gaaacggcgt ggcaggggga
      421 gtatgacgaa tggggaaacc tgctgggcga aaccagcgcg cagcacctcc agcagtcact
      481 ccgtctgccg gggcagcagt atgatgagga gtcggggctg tactacaacc gcaaccggta
      541 ctatgacccg ttgcagggaa ggtatatcac ccaggatccg atagggctga gaggtgaatg
      601 gaatctgtat aaatatccat taaaccctgt tcggtttata gattctttgg gtttaaaatt
      661 ccatgttaac ggtgatccaa gtgattttaa tcaggctgtt gagtatttaa aacaagattc
      721 acaaatgaaa gaaactatag attttttatc aagctctgaa gaaactataa atatagagta
      781 tattgaagga accaatgtta gatttaattc gaataatatg acgatatatt ggaatagtag
      841 agcatcttta ttttgttcaa ctgaattaaa ctcgaaatca caatcgcctg cattaggttt
      901 gggacatgag tttgctcatg cgcagtattg ccttcttgat aaggaaaatt tcatggctct
      961 tttaagccga acagataaga aatatgagaa taaagaagag gcaagggtta ttacaataat
     1021 tgaatcgcgt gcggcgaaaa ctttaggtga gtgtaccaga ggagctcata gtggactgcc
     1081 tttttatcgg gtggatggac cgttgcagac gatgaaaata acgggtacgc cagaatagtg
     1141 aggtttttta tgatgaaata tttgatggtt ttattgtctc tattttctgg ttcggtattg
     1201 gggatggggc gagtgaatga attgtgtgga atagatagcg tgaaaacgat agagataatt
     1261 aatcttccat cgtatgttac cacgttagtt ccgcttagca aggaaggtct taatgaaata
     1321 tacagatata aagtggtggt gaatgaaatt agagatcttt atgctggtaa gataattgat
     1381 ttattgcaaa tgaagtattt cagaaaagaa aaatataaca atataagatg ggggggtagt
     1441 atcatttcga aaggtaataa taaatgtgaa atttattttg atgcttttgg tgaatgtgga
     1501 agtgtgaatg gcattaacgt gtgttttgaa aaaaatgaaa tgataggttg gattaaaaaa
     1561 gaaatccctt tgctgtcgca aaaaataggt gggctttaaa tggtagggca atattcaaag
     1621 acagtatagg taactatttg tatggccatg taaggtaaaa gaaaaggaat aaaccatgag
     1681 cggaaaaccg gcggcgcggc agggc
//
LOCUS       DAZPEP010000325         1696 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZPEP010000325 DAZPEP010000000
VERSION     DAZPEP010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.327"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..547
                     /locus_tag="ACHUT9_004507"
     CDS             <1..547
                     /locus_tag="ACHUT9_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350790.1"
                     /translation="QIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQVEKVGIIRALT
                     VGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFSVGKTMKENTG
                     QTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAPVINWNCGATQ
                     PVPDAPVPKDLPPGMPDMRQF"
     gene            623..>1696
                     /locus_tag="ACHUT9_004508"
     CDS             623..>1696
                     /locus_tag="ACHUT9_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005148713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFR1350791.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLS
                     ALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAA
                     EGDVAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPD
                     TLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQV
                     RGFTYDAEHAGRMVAHHYAGRPES"
BASE COUNT          349 a          443 c          576 g          328 t
ORIGIN      
        1 ccagatccag aacattggcg ttaatcagat acagacggtt ggtgtcaacc aggtggaaac
       61 ggtgggcagt aaccagatta tcaaagtggg atcaaaccag gttgaaaagg tggggatcat
      121 tcgtgcgctg acggtgggtg tggcttacca gacgacggta ggcggcatta tgaatacctc
      181 ggtggcgttg ttgcagtcct cacaggtagg gctgcataaa tcactgatgg tgggaatggg
      241 ctacagcgtc aatgtaggga ataacgtcac cttctcggtg ggcaagacga tgaaggaaaa
      301 caccggacaa acagcagttt attctgccgg tgagcatctt gaactctgct gtggtaaggc
      361 aaggctggtg ctgacgaagg acggaagcat atttctcaac ggtacgcaca ttcatctgga
      421 aggggagtcg gatgtgaacg gtgatgcgcc agtgattaac tggaactgtg gtgccacaca
      481 acctgtaccg gatgcgcctg tgccgaaaga tttacccccc ggaatgccgg atatgcggca
      541 attttgagtc aggtgcaaaa aattaaaacg gaactggtgc agtgaccaga tgaaccatat
      601 taaaagcaga aggaattgat tcatgggcgg cggaaaaccg gcggcgcggc agggcgacat
      661 gacccgcaag ggactggata ttgtgcaggg ttcagcgggt gtgctgatag gtgcgccgac
      721 gggagtggcc tgttcggtgt gtccgggagg gattacctat gctaacccgg tgaacccggt
      781 gaacccggtg ctgggtgcga aggtgcttcc gggcgagacg gaccttgcgc tgccctgccc
      841 gctgccgttt attcttttcc gtgcctacag cagctaccgg accagaacac ccgcgccggt
      901 gggggtgttt ggtcccggct ggaaagcgcc cttcgatatc cgcttacaga tacgcgatga
      961 aggcctgata ctcaacgaca gcggcggcag gagcattcac tttgagccgc tgtttcccgg
     1021 cgagataagc tacagccgca gcgagtcact ctggctggcg cggggcggag tggcggcgca
     1081 gcacagttcg cagccgctaa gcgcgctctg gcaggtgctg ccggaagatg ttcgcctgag
     1141 tccgcatgtg tacctggcga caaacagcct tcaggggccg tggtggatac tcagctggcc
     1201 ggagccgccc gcataccggg tactgacggt cgtggtggat ggcttcgggc gctcactgac
     1261 tttccaccgg gcggcggagg gtgacgtggc gggcgcggtg acgggggtga cggacggcgc
     1321 gggccgccgt tttcacatgg cgctgagcac gcaggcgcag cgggcggaag cgtcccgtaa
     1381 acagcgcgcc tcttccttat cttccccggc cagtccccgt tctgtttcct cttctcaggt
     1441 tttccccgac acgctgcccg ccggtacaga gtacggtgcc gataacggta tccggctgga
     1501 ggcggtatgg ctgacacacg acccggcata cccggatgaa cagccgactg cgccgctggc
     1561 gcgctacacg tacacggccg gtggtgaact gcgggcggtg tatgaccgca gcgggacgca
     1621 ggttcgcggg tttacttatg atgcggagca cgccgggcgg atggtggcgc accattatgc
     1681 ggggaggccg gagagc
//
LOCUS       DAZPEP010000326         1680 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZPEP010000326 DAZPEP010000000
VERSION     DAZPEP010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.328"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..472)
                     /locus_tag="ACHUT9_004509"
     CDS             complement(<1..472)
                     /locus_tag="ACHUT9_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1350792.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCG"
     gene            480..981
                     /gene="tnpA"
                     /locus_tag="ACHUT9_004510"
                     /pseudo
     CDS             480..981
                     /gene="tnpA"
                     /locus_tag="ACHUT9_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(999..1667)
                     /locus_tag="ACHUT9_004511"
                     /pseudo
     CDS             complement(999..1667)
                     /locus_tag="ACHUT9_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          429 a          400 c          384 g          467 t
ORIGIN      
        1 taccgcagga ctggctgaca gtgacatttt tcacaacacc cgtgacctga cggctattcc
       61 ggtagcgcat ccagcccagt ttcggcagaa aaatacggct gttttcctga tcgagcttaa
      121 cgccctgcgg gtagcggaat gcatcattct gtccccgctt tttgaatcgg ggaaaagcag
      181 cccgcttccg gaagaagttt ttgtaagccc gctcaaggtc tttcagtgac tgttgcaatg
      241 gctgtgaggg agaatcttta agccattgcg tttcagtggc atttttccac tcaaccagcc
      301 aggaagccat tttcccgtaa gggatgtatt tattaccggc ctcatgattc tcattctgac
      361 gtgccagagc acgattgaaa acgaaacgac atgcgcctgc gaagcgcctc atttcacact
      421 cctgttgacc acctggtctt aactggaatt taaatgcctg taatcgtttc acaggtcaaa
      481 tgatactttg gtctgtgaaa aaagaaaccg atatccggcg tggaaggcat tgtgttttcc
      541 tgatgcatgt tcacctggtc tttgtcacta gataccgacg ccagattttt tgacgcgaca
      601 gaaaaactac gcacttactt ttcaaatgta tgtgcttatt ttgaagctga actggttgaa
      661 atggatggcg aaccagatca cgtccatttg ttaattaact aacctcccaa actggcgata
      721 tccagtctgg taaacagcct caaaggcgta tcgggtaggt tactgcgacg agatcgacca
      781 gatattgcag tcaggtatta ctacaaaggc gttttgtgga gtcctggcta ttttgccagt
      841 agctgcggag gtgtgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct
      901 ggtcaggtgg aaaaccgcgc cttatatccc cgccctgaag gatggggttt tacggcgcac
      961 cggataaaaa gcgccatcta acgatggcgc gtctggaact actttttgtt aacgtcaaac
     1021 atggtggcat cggtcgccat gtcatcaata acttgtttaa tattttcgaa agccattggc
     1081 gtactttcgt tattcaggtc tttaccttcg ccctgacgaa cgactttgat aaccggttta
     1141 ttagtcgctg catcaatcag ctcaccttca aaatagaggc gagtatccat ggtacggtgg
     1201 cctgtagcca tttgcgtccc cgcaaccact aatgcaacag gaaccacttc atagaattgc
     1261 agcccttctt tgctggtatc tacaccggta atggccccac ggaaaatcag actacgcggc
     1321 ccagcggtgg taaccagtgg tttacgctgc gctatcgctt ctttcatttc ggtgttggta
     1381 tagttcaaaa ttttatccag aactttctgc cctacctagg tagaaggttt tggcaccgga
     1441 taataagtga ttgggttcca gacgatgctg tcatatttgc tttgatcaaa actcgggtct
     1501 acccaacgta aaacaggttt acctgttgct gaggttgttt cttttaaatc agagtaattg
     1561 tttaaaaaac cagaatattt atctggctgg gtgattttag aggcacaacc agacagagcc
     1621 aacaagccgc ataaagctgc aactttcgca aatgatgtgg tacgcatgaa tagtttcctg
//
LOCUS       DAZPEP010000327         1669 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZPEP010000327 DAZPEP010000000
VERSION     DAZPEP010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.329"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            110..364
                     /gene="bssS"
                     /locus_tag="ACHUT9_004512"
     CDS             110..364
                     /gene="bssS"
                     /locus_tag="ACHUT9_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFR1350793.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            479..1597
                     /gene="solA"
                     /locus_tag="ACHUT9_004513"
     CDS             479..1597
                     /gene="solA"
                     /locus_tag="ACHUT9_004513"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFR1350794.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPEQPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT          434 a          411 c          413 g          411 t
ORIGIN      
        1 ttaaaaattc ggtgaatacc cttacttatt ggtatactga agttgcttca gataaaaacg
       61 cattgaacct cgaataacgt tgtctagtaa cacgaattag ggggccatga tggaaaaaaa
      121 taatgaagtc attcagactc atccgctcgt agggtgggac atcagcaccg ttgatagcta
      181 tgatgcgctg atgttgcgtt tgcactacca gaccccaaat aagtccgagc aggaagggac
      241 tgaagttggt caaacgctct ggttaaccac tgatgttgcc agacaattta tttcgatatt
      301 agaagcagga atcgccaaaa ttgaatccgg tgatttccag gtaaacgagt atcggcgtca
      361 ttaacatgac atgaccatcc acccaaaagg cacctcaccg gtgcctttac catttctggg
      421 ttgtatatct gcattcagtt aattactctg cttaaaccat gagagcagag agaaaaaaat
      481 gaaatacgat ctcatcatta ttggcagcgg ttccgtaggc gctgccgccg ggtattatgc
      541 aacccgcgcc ggtttaaacg tgctaatgac cgacgcccat atgccaccgc atcaacacgg
      601 cagccaccac ggcgatacgc gattaattcg ccatgcttat ggtgaaggcg aaaagtatgt
      661 cccgctggtc ctccgcgcgc aaacgctgtg ggatgaactt tcccgccaca acgaagatga
      721 tcccattttt gtacgctctg gtgtcattaa cctcggcccg gctgactccg catttctcgc
      781 caacgtcgcc cacagcgccg aacagtggca actcaacgtt gaaaagctcg atgcgcaagg
      841 gattatggcc cgctggccag aaatacgcgt cccggacaac tacatcggct tatttgagac
      901 tgattccggt tttttgcgca gcgaactggc gattaaaacc tggatccaac tggcgaagga
      961 agcgggctgt gcgcaactgt tcaactgccc ggtcaccgca attcgtcatg acgatgatgg
     1021 cgtaactatt gaaacggttg acggtgagta tcaggcgaaa aaagcgattg tctgcgcggg
     1081 aacatgggta aaagacctgc tcccggagca gcctgtccag cccgtacgta aagtatttgc
     1141 ctggtatcag gccgatggcc gctatagcgt gaagaataaa ttcccggcgt ttaccggtga
     1201 actgcccaat ggcgatcaat attatggttt tccggcagaa aacgacgcgt tgaagattgg
     1261 caaacataac ggaggccagg ttatccattc agcggatgaa cgtgttccgt ttgcggaagt
     1321 ggtcagcgat ggttcggaag ccttcccgtt cttgcgcaat gtattgccgg gtatcggttg
     1381 ctgcctgtac ggcgctgcct gcacctatga taattcgcct gacgaagatt ttattatcga
     1441 taccctaccc ggccacgata atacactgct cattaccggc ctgagtgggc acggttttaa
     1501 atttgcgtca gttttagggg aaatagctgc cgattttgcg caagacaaaa aaagcgattt
     1561 tgatttgacg ccattcaggc tttcccgctt ccaataatca tcattacggc ctccagaatg
     1621 aggccgtttt tacaggagta tttaatgcgt cggctgttgc actatctca
//
LOCUS       DAZPEP010000328         1657 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZPEP010000328 DAZPEP010000000
VERSION     DAZPEP010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.330"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            211..>1657
                     /gene="istA"
                     /locus_tag="ACHUT9_004514"
     CDS             211..>1657
                     /gene="istA"
                     /locus_tag="ACHUT9_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HFR1350795.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTRAERFRMLDAPALSPLPLEPYEYTEYKAVKVGPD
                     YHVEYARHWYSVPHELVGQRLSLKVGQSVVQLWHKGQCVAQHPRSTHEYKHTTNPLHM
                     PERHRRHGTWTPERLIEQGNRTGPSTGRVVESMLKAKPHPELAYRAVLGLLALQKKYG
                     PERLEKACYVALHYNAPDRRFIDNLLRHHRDN"
BASE COUNT          419 a          463 c          414 g          361 t
ORIGIN      
        1 atcgcagttt ggcattcgaa tcgatcatga tttggcatcc atctccgatc acagtttggc
       61 atcacaatcg atcacgattt ggcatacttc cgatcattga ttggaattcg ccagtcactc
      121 cgggaattaa ctcttttcac cacagtctcc attgccgtgt ttaaaccagt ggagacgtca
      181 atgtccaaaa agagaaaatc caggagcact atggaaactt gttttaagat ccttcagctc
      241 aagttcgata agaagctgac taaccgttgt atcggactga cgttacacat cagcgcctca
      301 accgtttttg aggttcttgc ccgcttcaaa gcctcttctc tgtcatggcc attacctgcg
      361 gatatatccc atgacacgct ggagaaactc attttcccgc ccaaagacac ctctgcgtca
      421 gagcttgtta tgcctgacat gctgtacttc gatacggaaa tgagaaaacc tggcgtgacg
      481 cgccaactgt tgtggatgga atacaaggca caggcgggtg acaaagcgat gggatactca
      541 cacttctgcc gttgctaccg gaagtggaaa aaaacgcgac gcctctccat gcgacaggag
      601 catcgtgctg gcgaaaagct ctttatcgac ttctgtggtc ctacggtccc tgttattaat
      661 ccggacaccg gagagatacg ccgcgtggca atcttcgtgg ctgtaatggg ggcgtccaac
      721 tatacctatg ttgaagcgtg cgaaggtcag gacatgatgt catggttaaa tgcccacagc
      781 cgttgcctga ccttcctggg cggcgtaccg aagctgctca tccccgataa cctcagaagc
      841 gcagtgaaaa aagccgatcg ctatgagcct gtcatcaacg acagttatca ggcgctggct
      901 gagcactatg gcacggttat tatcccggcc cgcccgcgca aacctaaaga taaaccaaaa
      961 gcagaaaacg gcgtcctcat cgtcgagcga tggcttcttg cccgcatccg taatgaaacc
     1021 ttccacacgc tcagggcgct gaatgcacgc cttcgggagt tgctgacgga catgaataac
     1081 cgaccaatga agggatacgg taatcaaacc cgcgcagaac gcttccgcat gctggatgca
     1141 ccagcactct ctccgctgcc tctggagcct tatgaataca cggaatacaa agccgtgaag
     1201 gttggccctg actaccacgt tgaatatgcc cgtcactggt actccgttcc acacgaactg
     1261 gtcggtcagc gtctgtcact gaaggtgggg cagtcggtgg tacagctctg gcacaagggg
     1321 cagtgtgtgg cgcaacatcc gcgcagcaca catgaatata aacacacaac caatccgctt
     1381 cacatgccag aacgccaccg gcggcatggg acatggacac cggaacgcct tatcgagcag
     1441 ggaaaccgta caggtccgtc cacaggccgg gtggttgaat ccatgctgaa agccaaaccc
     1501 catccggagc tggcttaccg tgcagtcctg ggtctgctgg cacttcagaa aaaatacggc
     1561 ccggaacggc tggaaaaggc ctgttacgtt gccctgcact ataacgcccc ggacagacgc
     1621 tttatcgaca atctgctgcg tcatcatcgg gataacg
//
LOCUS       DAZPEP010000329         1629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZPEP010000329 DAZPEP010000000
VERSION     DAZPEP010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.331"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHUT9_004515"
     CDS             <1..93
                     /locus_tag="ACHUT9_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFR1350796.1"
                     /translation="RFYLEEQGWTLVNRGAIKNMTVEQLLKIHC"
     gene            104..292
                     /gene="dicB"
                     /locus_tag="ACHUT9_004516"
     CDS             104..292
                     /gene="dicB"
                     /locus_tag="ACHUT9_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFR1350797.1"
                     /translation="METLLPNVNTTEGCFEIGVTISNTVFTEDAINKRKHERESLNKI
                     CIVSMLARLRLMQKGCWR"
     gene            289..480
                     /locus_tag="ACHUT9_004517"
     CDS             289..480
                     /locus_tag="ACHUT9_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFR1350798.1"
                     /translation="MNTAFALVLTVILNTGEPAELVTGVYNSMKECVAAAAEQKIPGN
                     CYPVDKIIHQDNKEVPAGL"
     gene            574..>1629
                     /locus_tag="ACHUT9_004518"
     CDS             574..>1629
                     /locus_tag="ACHUT9_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFR1350799.1"
                     /translation="MSKVFICAAIPDEKATKEEGAKPVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESEPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMCPEIKLHAIGWIKHKCKPGAKWPEIQAEMRIWKKCREGERKETGKYTSVVDLARA
                     RANQQHTENSTGKISPAIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATD"
BASE COUNT          498 a          361 c          391 g          379 t
ORIGIN      
        1 cggttttatc tggaagaaca gggatggact cttgttaacc ggggcgcaat taaaaatatg
       61 actgtagagc aattactcaa aattcattgt tgaggacgcg ataatggaaa cgttattacc
      121 aaacgttaat acgactgaag gttgttttga aattggtgtc actatcagta acacggtatt
      181 tactgaagat gccattaaca agagaaaaca cgaacgggag tcattaaata aaatatgcat
      241 tgtttcaatg ctggctcgtt tgcgtctgat gcaaaaagga tgctggcgat gaatacagca
      301 tttgcactcg ttctgaccgt tattcttaat acgggcgaac cagctgaact ggttaccgga
      361 gtatacaact ccatgaaaga atgcgtggct gctgcagctg aacagaaaat tcccggtaac
      421 tgttatccgg ttgataaaat cattcaccag gataataaag aagtcccggc aggtctttaa
      481 agcagccacg taataaatat ccggtttcat ttttatatgc cagcaatggc agggatttgt
      541 tcacccttaa atctgtaatg aggttaaaac aaaatgagta aagtctttat ttgcgccgcc
      601 attccggacg aaaaggcaac aaaagaagaa ggcgctaaac ctgtagccac tgccattgaa
      661 gccggcgacg aacgccgcgc ccgcgcaaaa tttcactggc aattcctgga acattatccg
      721 gctgctcagg actgcgctta taaatttatt gtctgcgagg ataaacctgg tataccccgc
      781 cctgccctcg attcctggga tgctgaatat atgcaggaaa accgctggga tgaggagtct
      841 gcttcctttg tcccggttga gactgaatcc gaaccgatga acgtcacttt tgacaagctg
      901 gcccctgaag tacagaacgc tgtcatggtt aagttcgaca catgtgaaaa catcaccgtt
      961 gatatggtga ttagcgcgca ggaactgttg caggaagaca tggcaacatt cgacggacat
     1021 atcgttgaag cgttgatgaa aatgccagaa gttaacgcca tgtgtccgga gattaaactg
     1081 catgccatcg ggtggattaa gcataaatgt aagcctggtg caaaatggcc agaaattcag
     1141 gcagagatgc gcatctggaa aaaatgtcgc gaaggtgaac gcaaggaaac cggaaaatac
     1201 acgtctgttg ttgatctcgc ccgtgccaga gccaatcaac agcacactga aaattcaaca
     1261 ggaaaaatca gcccggccat tgctgccatt catcgcgaat acaagcagac atggaaaaca
     1321 ctggatgacg aactggccta cgctctctgg cctggtgatg tggatgccgg aaacattgac
     1381 ggcagcatcc atcgctgggc aaaaaatgaa gttatcgaca acgaccgcga agactggaag
     1441 cgtatctcgg catcaatgcg caaacagcct gatgcccttc gctacgaccg ccagactatt
     1501 tttggccttg tccgtgaacg tccgatcgac attcacaaag accctgtggc actgaacaaa
     1561 tacattactg aatacctgac tacaaagggc gtgtttgaag atgaaggaac aaatcagagc
     1621 gcaactgat
//
LOCUS       DAZPEP010000330         1572 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZPEP010000330 DAZPEP010000000
VERSION     DAZPEP010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.332"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            140..1572
                     /gene="rhsC"
                     /locus_tag="ACHUT9_004519"
                     /pseudo
     CDS             140..1572
                     /gene="rhsC"
                     /locus_tag="ACHUT9_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          391 a          453 c          476 g          252 t
ORIGIN      
        1 tccctgagga aaaatccccc gacagtggaa agcagcgaaa aaacgagtta aaaagcggta
       61 gaaaaagtta agttcccggt tgatcgaatg gatgcgaatc gctgatcgaa cttcgatgcc
      121 aatccgtgat cgaatagagc ggaatacgca gctggtactg accacgcagg cgcagcgggc
      181 agaagaagcc cggcagcagg ccatttccgg cgggacggaa ccgtccgctt ttcctgatac
      241 cctgccgggt tacaccgaat atggccggga caacggcatc cgtctgtctg ccgtgtggct
      301 gacgcacgac ccggaatacc cggagaattt acctgccgcg ccgctggtgc gctatggctg
      361 gacgccgcgc ggcgaactgg cggcagtgta tgaccgcagc aatacacagg tgcgcagctt
      421 tacttacgat gataaatacc ggggccggat ggtggcgcac cgtcacacgg gccggccgga
      481 aatccgttac cgttacgaca gcgacgggcg ggtgacagaa cagctaaacc cggcaggctt
      541 aagctacacg tatcagtatg agaaggaccg catcaccatc accgacagcc tgaaccgccg
      601 tgaagtgctg cacacgcagg gtgaagccgg gctgaagcgg gtggtgaaaa aggaacacgc
      661 ggacggcagc gtcacgcaga gtcagtttga tgcggtgggc aggctcaggg cacagacgga
      721 tgccgcaggc cggacgactg agtacagccc ggatgtggtg acgggactca tcacgcgcat
      781 caccacgccg gatggcaggg catcggcgtt ttactataac caccacagcc agttaacgtc
      841 agccaccggg cctgacgggc tggaaatgcg ccgcaaatat gatgaatccg gccgtctgat
      901 tcaggaaact gcccctgacg gcgatatcat ccgctaccgt tatgataatc cacacagtga
      961 cttaccctgc gcaacggaag atgccaccgg cagccggaaa accatgacgt ggagccgtta
     1021 cggtcagttg ctgagcttca ccgactgttc cggttatgta acccgttatg accatgaccg
     1081 cttcgggcag atgacggcgg tgcaccgcga ggaagggctg agtcagtacc gcgcatacga
     1141 cagccgtgga cagttaattg ccgtgaaaga cacgcagggc catgaaacgc ggtatgaata
     1201 caacgccgcc ggtgacctga ccaccgtcat tgccccggac ggcagcagaa acgggacaca
     1261 gtacgatgcg tggggaaaag ctatctgtac cacgcagggc ggtctgacgc gcagtatgga
     1321 atacgatgct gccggacggg tcatccgcct gaccagtgaa aacggcagcc acaccacctt
     1381 ccgttacgat gtactcgacc tgctgataca ggaaaccggc tttgacggcc gcacacagcg
     1441 ttatcaccac gacctgaccg gcaaacttat ccgcagcgag gatgaggggc tggtcaccca
     1501 ctggcactat gacgaagcag accgcctcac gcaccgcacc gtgaatggcg aaaccgcaga
     1561 gcggtggcag ta
//
LOCUS       DAZPEP010000331         1547 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZPEP010000331 DAZPEP010000000
VERSION     DAZPEP010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.333"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            88..531
                     /gene="cmtB"
                     /locus_tag="ACHUT9_004520"
     CDS             88..531
                     /gene="cmtB"
                     /locus_tag="ACHUT9_004520"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFR1350800.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            559..>1547
                     /gene="cmtA"
                     /locus_tag="ACHUT9_004521"
     CDS             559..>1547
                     /gene="cmtA"
                     /locus_tag="ACHUT9_004521"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFR1350801.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
BASE COUNT          358 a          345 c          390 g          454 t
ORIGIN      
        1 ttccctgctc aattgctttc attaaaccgc acgagtattt tatttttacc gccggacggt
       61 accctaaaaa taaaataagg ataatttatg cggcttagtg actattttcc agaatcatca
      121 atctcagtta tacattcagc aaaagattgg caggaagcta tcgatttctc gatggtatca
      181 ttgctggata aaaactatat cagcgagaat tacattcagg caattaaaga ttccaccatt
      241 aacaatggcc cttattatat tctcgcacca ggcgtggcaa tgcctcatgc gcgaccggaa
      301 tgtggggcgc ttaaaaccgg gatgtctttg acattacttg aacaaggtgt ttattttccg
      361 gggaatgacg aaccaataaa attactcatc ggcctttctg ctgccgatgc cgattcgcac
      421 attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag aagaaatact cgaacaactc
      481 ttaacagcag catcagaaaa acaattagcg gacataatca gccgcggata attactctct
      541 cctctacagg ataatattat ggaaaacaag tctgctcgtg caaaggtcca ggcttttggg
      601 ggctttttga cggcaatggt catccccaat attggtgctt ttattgcctg gggttttatt
      661 actgcgttat ttattcccac cggttggctg cctaacgaac atttcgccaa aattgtcggc
      721 ccgatgatta cctatttatt gcccgtgatg attggttcta ccggtggtca tctggtcggc
      781 ggtaaacgcg gcgcggtcat gggcggaata ggtactattg gtgtgatcgt cggcgcagag
      841 atcccgatgt tccttggctc aatgattatg gggccgctcg gtgggctggt cataaaatat
      901 gttgataagg cactggaaaa acgcatacct gccggttttg agatggttat caataacttc
      961 tcattaggta tcgcggggat gctcctttgt ctgctgggtt ttgaagttat cggcccggcg
     1021 gtgttaattg ccaatactct cgtcaaagag tgtattgagg cgctggtaca tgcgggttat
     1081 ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc ttttcctcaa taatgcgatc
     1141 gatcagggcg tctattatcc gctgggaatg caacaggctt cggttaacgg taaatccatc
     1201 ttctttatgg tggcgtccaa tcctgggcct ggcttagggc tgctgctggc gtttaccttg
     1261 tttggtaaag ggatgagtaa acgttctgcg cccggggcga tgattattca cttcctcggt
     1321 gggatccacg aactgtattt cccgtatgtg ctgatgaagc cgctgaccat tattgccatg
     1381 attgcgggcg gtatgtctgg cacctgggtg tttaacttac tggacggtgg tctggtggct
     1441 ggcccaagtc cggggtctat ctttgcttac ctggcactga cgccgaaagg ctcgttcctg
     1501 gcgacaattg ccggtgttac ggtagctacc ctggtgtcct ttgctat
//
LOCUS       DAZPEP010000332         1546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZPEP010000332 DAZPEP010000000
VERSION     DAZPEP010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.334"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(918..1394)
                     /locus_tag="ACHUT9_004522"
     CDS             complement(918..1394)
                     /locus_tag="ACHUT9_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFR1350802.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          351 a          415 c          384 g          396 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gttcaacagc tcgatcatgt ttttgccgtc cgttcaccgt taaaacgcct
      241 cagaaatcgg gacaggatgt gggaatgtcg gaccgcgcac gcgcactcag acattcccca
      301 cctggtcagg gcttcacccc gacaccccgc atccgtgcgt aagtgctcaa tgtaacttcc
      361 ggttctcttc cgcttcctcg tcgattcgct cgctacgctc ggtcatcgac ttgcggcgag
      421 cgggatcacc tctcgcatgt tgtcggtgat cccgttgttg ttctttcggg gatgagtcgt
      481 cagatagccc ttcaaacacg ctcagaagct ctcagacgca cgaaacgggt ttaaatgccc
      541 gccagggcat ggaaggcgcg aaaaacgctt acagaggctc tcaggaggtc aggcgcgccg
      601 atacccctaa ccaggggctt tcaggtcgct aggaaccgcg tcagctccct gcaaagccta
      661 tttgcacggc acaggcagct attcggctgg ttggtggtcg tagaccgtcg gtgttacagc
      721 gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg ctgccggaac
      781 cgtggcggcg cggtcgttct ggggatcggg gagcaaaaac acgcaaggga ttttgaggcg
      841 gcaaaagagc aatgcaggat agacaatcat ggtcaaaacg ttaacacgta gcccctgatt
      901 gtccgcctgc cgctggctca atttgaaaac atggccatga ttcggcgctg aatatcaccg
      961 tctttgttga ccagtgtttt agtgacaaac gctgcatggt ttttcgtagt gttgtatccc
     1021 ttccagactt catgtggatc gagggcatac gcacaaacct taccagcaac ggggattcta
     1081 actaatgcat tttttgattc taagtaattg agccaccgaa taattgtgct tctgctaact
     1141 ccaagttttt cagctaggaa ttgctgatcc gcaacgacag cgccacacgt aggatcgata
     1201 tgctccgcta aaaatgagta cagcttcagt gctgatatgc cttgactatc cttcagcagc
     1261 tctcgaacac gttcccaacc ttttggggat acctgtgtaa accttgaatt cttctttgct
     1321 tcttcgagat cctcggtgtt tttctttgcc tcatctgaaa gttgtttcag tcgtttttct
     1381 gaatcaataa acatgatttt ttccctctga aaatcccatg cactaacgtt ggttcaaatc
     1441 taagccatca tgacacatct gtgcaacacc ttgttgcgtg ttgtcgcatc ctggatgcga
     1501 caacgttgtg tcaccctgga tgcgacaggt aaacaaaaaa cacact
//
LOCUS       DAZPEP010000333         1533 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZPEP010000333 DAZPEP010000000
VERSION     DAZPEP010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.335"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>1533
                     /locus_tag="ACHUT9_004523"
     CDS             <1..>1533
                     /locus_tag="ACHUT9_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFR1350803.1"
                     /translation="EAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGAVAIDDAGYDED
                     DFKKPVRVNLPVVGDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSPSVQDKPASTDN
                     TNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEELP
                     AVTALAVSHKTSLLDPLEIRDLHKLVRDTDKVFPNPGNSSLGLITAFFEAYLNADYTD
                     RGLLTKEWMKGNRVSHITRTASGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSM
                     DLDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGI
                     EVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPS
                     CAMADEQATAETVEPDATEHHQDTQPLDAQSQVNAVDAKYQKLRTELHEARKNIPPKN
                     PVDADKLLAASRGEFVEGISDPNDSKWIPGHHISSNEVKKTENKVRQTEDKQHQNSEP
                     EE"
BASE COUNT          439 a          400 c          371 g          323 t
ORIGIN      
        1 acgaagcaaa aaatgacaaa gtaagaaaac gcctcggtat taaaggcggt ttttactggg
       61 ctgaagcgaa aaaattatcc attgccatct cccgtggtgc tgttgcgatt gacgatgctg
      121 gctacgatga agatgacttt aaaaaacctg ttcgcgtcaa tttgcccgtt gttggtgacc
      181 tcccaccaga aggcgtgttt gatacggaat tctgcaaccg ttacgaaaaa ggcggggaag
      241 atggcatcac aatggtattt atcgcatctt ctccctctgt tcaggacaaa ccagccagca
      301 ctgacaatac caacgtcaac ggcgaagaca tgacagaaat tgaggagaac atgctcctgc
      361 cagtttccgg tcaggaactg cccattcgct ggcttgctca acacggcagc gaaaaaccgg
      421 taacgcacgt ttcacgcgac ggactccagg cattacacat tgctcgggct gaagaactac
      481 cggctgttac tgccctggct gtttcccaca aaaccagcct gctcgacccg ctggaaattc
      541 gcgatctcca caaactggtt cgtgacactg acaaagtttt ccctaatcct ggtaattcaa
      601 gcctgggact gataactgct tttttcgaag catacctgaa cgctgactac accgatcgag
      661 gactgctgac aaaagagtgg atgaagggta atcgtgtttc acacatcact cgcacggctt
      721 ccggtgctaa tgctggcggc ggaaacctca ccgatcgcgg cgaaggtttc gtacacgatc
      781 tgacgtcact ggcgcgcgac gtagccactg gcgtactggc ccgttcaatg gatctggaca
      841 tctataacct tcatccggca cacgctaaac gcattgagga aattatcgct gaaaataaac
      901 cgcccttttc tgttttccgc gacaaattca tcaccatgcc tggcgggctg gattattccc
      961 gcgccatcgt ggttgcgtcc gtaaaagaag caccaattgg gatcgaggtc atccccgcgc
     1021 acgtcactga atatctgaac aaggtactga ctgaaaccga tcatgccaac cctgatccgg
     1081 aaatcgtgga tattgcctgc ggtcgctcct ctgccccgat gccgcagcgt gtaacagaag
     1141 aaggaaaaca ggatgatgaa gaaaaaccgc aaccatcttg cgcaatggca gatgaacagg
     1201 caacggctga aacagtggaa ccggatgcaa ctgaacatca tcaggacacg cagccgctgg
     1261 atgctcagtc acaggtaaat gctgttgatg cgaaatatca gaaactgcgg acagaactcc
     1321 atgaagcccg gaaaaacatt ccgcccaaaa atcctgtcga tgcagacaaa ttactggctg
     1381 cctctcgcgg agaatttgtt gaagggatta gcgacccgaa tgattcgaaa tggattcccg
     1441 gtcatcatat ctcctcaaat gaagtcaaaa aaacggaaaa taaagtgcga cagactgaag
     1501 ataaacagca ccagaacagc gaaccggagg agc
//
LOCUS       DAZPEP010000334         1525 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZPEP010000334 DAZPEP010000000
VERSION     DAZPEP010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.336"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            97..360
                     /locus_tag="ACHUT9_004524"
                     /pseudo
     CDS             97..360
                     /locus_tag="ACHUT9_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701870.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            complement(397..954)
                     /locus_tag="ACHUT9_004525"
     CDS             complement(397..954)
                     /locus_tag="ACHUT9_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1350804.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(951..1226)
                     /locus_tag="ACHUT9_004526"
     CDS             complement(951..1226)
                     /locus_tag="ACHUT9_004526"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HFR1350805.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1412..1525
                     /locus_tag="ACHUT9_004527"
                     /pseudo
     CDS             1412..1525
                     /locus_tag="ACHUT9_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
BASE COUNT          424 a          325 c          367 g          409 t
ORIGIN      
        1 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
       61 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      121 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      181 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      241 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      301 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      361 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      421 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      481 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      541 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      601 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      661 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      721 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      781 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
      841 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
      901 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
      961 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1021 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1081 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1141 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1201 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1261 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1321 caggcagtta aaaacacgga aaaacggtat aaaaaagcgc ctcaaaatcc tgttttttct
     1381 ttcagataca atgtcttatt gcatttttta aaacatgtca gtgtggtaca tggatatcga
     1441 taccatcgcc aattataaac tgggcggtgc acagcaacat gatagcgtac gcctcgatcc
     1501 gtgggtgttt atgttctcag cagga
//
LOCUS       DAZPEP010000335         1483 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZPEP010000335 DAZPEP010000000
VERSION     DAZPEP010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.337"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHUT9_004528"
     CDS             complement(<1..1012)
                     /gene="cadA"
                     /locus_tag="ACHUT9_004528"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1350806.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHUT9_004529"
                     /pseudo
     CDS             complement(1092..1460)
                     /gene="cadB"
                     /locus_tag="ACHUT9_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="antiporter protein responsible for lysine import
                     and cadaverine export; member of the lysine-dependent acid
                     resistance system 4 (AR4); inner membrane protein;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
BASE COUNT          414 a          324 c          371 g          374 t
ORIGIN      
        1 tggtgtaagg cacccacgcg gagtcaaagt ggatggattt cacatccagt gttttcttga
       61 tgaagtcggt gttgtacagc agaccatcat aggtagagtt ggtaattaca gcatgtaccg
      121 gccaggttgc gtttggtgtt tctttcacgc gcttagcaat ggtagcgtgc tggaattcac
      181 tctgtgggat accaccaaga ataccgtaag cgttacgggt cgggcggaaa tagattggcg
      241 taacatcgct catcatcatc aggtgggtca gcgatttgtg gcagttacgg tcaatcagaa
      301 tggtgctgcc tgccggagca gagtacatgc caacaatttt gttcgcagtg gaagtaccgt
      361 tggtcaccat gtagctgcgg tctgcgttaa agacgcgagc gatatactgt tctgcttctt
      421 tgtgtggacc actatgatcc agcagagaac ccagttcaga tactgaaatg gaaatatcag
      481 atttcatggt attcggacca aagaaatcat agaacaggct acctaccggg cttttctgga
      541 atgcagtacc gcccatgtga ccaggagtac agaaagtata tttaccttca cgaacatatt
      601 taaacagtgc tttagtcagc ggaggcagaa tagtgttgat atattcgtca gtggtctgct
      661 tgatcttatt agcaatatct tcagcagcac ccagcgcata ttcaaagaag ctaatctgta
      721 aacgcaggtc attcaggctt acatcgagag tggaatacgt attagcgaac gcgtacaacg
      781 gcaggttctc gttcattttg ctaatttctt cgcacagctc gagattatat ttatcccagt
      841 caaaaataac gccgcacaga cgcgcattgt tttcgatcag ttttaataag tcgtcacggt
      901 cgttcgggta aacaatctgg aagttcagac gttcaagcgc gcgatgaagt tcacggatgg
      961 gttcttcttt aaaataaacc cccatgtgat tcaatattgc aataacgttc atagtcatat
     1021 ctccaggtaa aaaaggcccc tcccaacaca tgggacaaaa tgaaaggagg agcctcggaa
     1081 aatactttta attaatgtgc gttagacgcg gtgtggttat ccattgagtg gctctggcgc
     1141 tcgtgcattt tgcgagcgta gaacatcagg ataatcaggc tgacgatgaa ggtacctgcc
     1201 agctcgaagg agcttgcgcc catcagcgcg atgaagcaga acacacaacc cagtacagag
     1261 cagatcaggc tgacaaagtt gcggatgtta acgccttcaa aacgaatcag gtcaacgcaa
     1321 gagtagaagt acggcagcat agtcagcagt actgcgatac cggtcagttc accgaacagg
     1381 tcagatgctt taccaccggc agagttcatc agagtgatga ggatcatcag ggcagtcatt
     1441 ttcactgcag ccagcagcag taatgccgat cagttaagga tca
//
LOCUS       DAZPEP010000336         1473 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZPEP010000336 DAZPEP010000000
VERSION     DAZPEP010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1473
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.338"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..264
                     /locus_tag="ACHUT9_004530"
     CDS             <1..264
                     /locus_tag="ACHUT9_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1350807.1"
                     /translation="AEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSG
                     IRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            275..712
                     /locus_tag="ACHUT9_004531"
                     /pseudo
     CDS             275..712
                     /locus_tag="ACHUT9_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1089
                     /locus_tag="ACHUT9_004532"
     CDS             712..1089
                     /locus_tag="ACHUT9_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350808.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
BASE COUNT          486 a          214 c          373 g          400 t
ORIGIN      
        1 gcagaaaagt tcgccacagc aatccgaaac cactggcacg tggagaataa gctgcactgg
       61 cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa cgccgcagaa
      121 ttattttcag ggatacggca catcgctatt aatattttaa cgaatgataa ggtattcaag
      181 gcagggttaa gacgtaagat gcgaaaagca gccatggata gaaactatct cgcgtcagtc
      241 cttgcgggga gcgggctttc gtaatctttc cctagggagt cagccgaatg ttgggaagga
      301 tttgacggat gaagagaaga actgcttggc cggcggcagt acgggaagcc cgggaggatg
      361 gggaccagaa gatgagaata gcgcaaggga tcgtatgcag catgaacggt ataaagatga
      421 attgcgacag aaaatgggaa aacccgacgt tacagattca aatttgaaaa atattataga
      481 cgatctttat cggccaaatg caaaagtagg aagtggaagc acagcagatg ctgttcgtta
      541 tgaattggcc acaggtgaaa aagttggtgg tagaggacat gtattgaaag cacaggatta
      601 tacaagggct ttacaggatt ggttggataa gaatccaaca gcatctccag gggatcgagc
      661 agcagcagaa aacgttcttc aggatttaca gaatgctttg ggaggcaagt aatgaaccaa
      721 tatattgcta gttatataga aaaaatggca tcattttcca ctgttttgtc tgagacagtc
      781 tcattagcta atgaatactg gcatccagaa acgccaccgg tggttatgtt gttctctcag
      841 gttggaaaaa cgattgtaaa aaatttttat gaaatggggg atattgaaag agaaaaaata
      901 tttaaacatg tcgaagatgg tatggtctcg cttgatgatg aattggccac agttgtagct
      961 acagggctta tagaggcaat agtcactgcg accgatagca attctttact ttggcaagat
     1021 ttaagtaaat ttctgggtga caaatcaaag tctcatgcat tagtgtggaa aaattttggt
     1081 caggagtgat attattcaag aagtattaag ctcgctattg agatcttaat gcttcttgag
     1141 attaaattaa agttaggacg aagtaattgc aggtggaaat aaatccatag tgacgtgtgg
     1201 caatggctat tagatgttat ggtgtgatgg gctttggtga tttaagagct aagttagatt
     1261 gccttatgaa aaagtatggt tatatattta atgatgtgta tcatatattt aaataaaaaa
     1321 cggttctctc ttgtcaagat agtaatagga tagactattg tgttgataaa tataattaag
     1381 agtctacttt gttgatttta gaagactttg gagcagggaa agatcatcgg aagtacactt
     1441 tttgtactaa ataattcgcg ctaaatgttc aat
//
LOCUS       DAZPEP010000337         1462 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZPEP010000337 DAZPEP010000000
VERSION     DAZPEP010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.339"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1104
                     /locus_tag="ACHUT9_004533"
     CDS             <1..1104
                     /locus_tag="ACHUT9_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFR1350809.1"
                     /translation="LAWAVFKIFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTF
                     TAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTA
                     THNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNF
                     LAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGY
                     VTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPG
                     QVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYA
                     QVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          358 a          330 c          390 g          384 t
ORIGIN      
        1 ctggcgtggg cggtctttaa aatctttcgt attccggtga atcagtggac gctggcgacg
       61 gcggcgctgg gaggcgtgtt tctggtgagt ggtttgattt tgttgatgaa ctacaaccac
      121 ccttacactt ttaccgcgca aaaggcagtg atagcgatcc ctatcacgcc acaggtgacg
      181 ggaattgtta ctgaagtcac tgacaagaat aatcagctta ttcaaaaggg cgaggtgctt
      241 tttaagctcg acccggttcg ttaccaggcg cgagttgaca gacttcaggc tgacctgatg
      301 acggcgacgc ataatataaa gacgctgcgt gcgcagctca ctgaagcgca ggccaacacc
      361 acccaggttt cagcggagcg cgaccgtctg tttaaaaatt atcaacgtta cctgaaaggc
      421 agccaggcgg cggtgaatcc gttctcggaa cgtgacatcg acgatgcgcg gcaaaatttc
      481 ctcgcgcagg atgcgctggt gaaaggctcg gtggcggagc aggcgcagat ccagagccag
      541 ctcgacagta tggttaacgg cgagcaatcg cagattgtga gcttaagagc gcaacttact
      601 gaagcaaaat ataaccttga gcagactgtc attcgcgcgc cgagcaatgg ctacgttact
      661 caggtactga tccgcccagg tacatacgca gctgccttgc cgctgcgtcc ggtgatggtc
      721 ttcatccccg agcaaaaacg gcaaattgtc gcccaatttc ggcaaaactc gctgttacgt
      781 ctgaaacctg gcgatgatgc ggaagtggtg tttaacgcgc tacctgggca ggtgttccac
      841 ggcaaattga ctagtatttt acctgtcgtg ccaggcggtt cttatcaggc gcagggggta
      901 ttgcaatcat taacggtcgt tcccggcacg gacggcgtgc tgggaaccat tgaactggac
      961 cctaacgatg atatcgatgc cttacccgac ggcatttacg cccaggtagc agtttactcc
     1021 gaccatttca gccatgtttc ggtgatgcgg aaggtgctgc taagaatgac cagttggatg
     1081 cattatttgt atttggatca ttgaggggga agggggagat tatggaactt tgctccctgc
     1141 aagggctaat tatttttgca tattacttca ttggctgcga tgatttttct ttaatattca
     1201 ttatgttaat aagttctgga tgagtgtgct ctcattggat tttttatccg tcttgttctg
     1261 ccgcaggatg agactataca tcgtcgagat cgcaatgatg atatgagttg ttcctcctgc
     1321 gacggctcta atcaaaaaag agcaggagtg cttttccatc tattgcgatg atggctttat
     1381 tatctgaagg atgacagtca catttaaaaa atatttaatc tatagatcac aatgtagggt
     1441 catggaatat atgtatacaa ct
//
LOCUS       DAZPEP010000338         1457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZPEP010000338 DAZPEP010000000
VERSION     DAZPEP010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.340"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(268..1011)
                     /locus_tag="ACHUT9_004534"
     CDS             complement(268..1011)
                     /locus_tag="ACHUT9_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFR1350810.1"
                     /translation="MPSIPSFVLSGLLLLCLPFSSFACATTSHISFSYAARQRMQNRA
                     RLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLKASDRRWQALA
                     ENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAK
                     LPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGE
                     TIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            1227..>1457
                     /gene="dinJ"
                     /locus_tag="ACHUT9_004535"
     CDS             1227..>1457
                     /gene="dinJ"
                     /locus_tag="ACHUT9_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFR1350811.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDA"
BASE COUNT          394 a          353 c          347 g          363 t
ORIGIN      
        1 tgttctcctt aattatgtaa ggagtatttg atcatgcata agcattaaaa aacagtttca
       61 agtaatgagg aatatctcaa attttaaaca taaaatgcga attatttagt acaaaaagtg
      121 tacttctgat gatctttccc tgggtgatgg aataacagga aagcggctcg caaaaacgca
      181 cagatttgta ggcatgataa gacgcgtcag cgtcgcatca ggcatctgcg caccaccgcc
      241 ggatgcggcg taaacgcctt atccgtccta caaaatcgtc tcttccgtca atatccgccg
      301 cgcgcccaaa aaatgatcct gccagaaagg ttcagctaac cgacttaccc gaatggtttc
      361 cccggtgcgc ggtgactcaa taaactgacc gtcgcccaga tacaccccca tatggtcggc
      421 aatctcgcgg ctgtggatat ggaaaaacag caaatctccc cggcgcaggt cgttattcgc
      481 cacaatggtt gcccgacggt agtgatacat ctcattggcc gtacgcggga gcttcgcctc
      541 gaggatcttg ttgtaggcat aaaaaactaa cccactacaa tcgaagcctt gatcgggccg
      601 cgtgccgccc cagacgtaag gcttgccaag ctgctgctcc agacggtgaa tcgccacttc
      661 ggtgatgtta tgcaggtgat cgctgcttaa aaagtggttg ttttccgcca gcgcctgcca
      721 gcgtctgtca ctcgccttga gcggcgcagg aaaccattct ggatgctgtt ttatctgctc
      781 ccggttgtgc tggcgcagcg cccttctgct ttgggcattg ccttccacaa tgtagctggc
      841 ctgttttttc agatgggttt ggtactgttt taataaacgc gcacggtttt gcatccgctg
      901 gcgggcggcg tagctgaaag agatatgtga tgtggtggcg caggcaaatg aagaaaacgg
      961 caagcaaagc aacagtaatc ccgacaacac aaaagaggga atcgacggca aagacattct
     1021 caataatggc tggtcatgca ttgatgttgc agtgtatcct ggcccgtacc tgaacaaact
     1081 taataaacca ttattataaa attaataaat atatttcagt ttattatgta aaaacatccg
     1141 tttattaaat tactcatgga ttgtcgctgt tgctcatttg agctacaatt caagctgaat
     1201 aaatatacag cacaggagat cccccaatgg ctgctaacgc gtttgttcgc gcccgaatcg
     1261 atgaagatct gaagaatcag gcggcggacg tactggccgg gatggggctg accatctctg
     1321 acctggttcg cataaccctc acaaaggtcg cgcgtgaaaa ggcattgccg tttgatttac
     1381 gcgagcctaa tcaattaacc attcaatcaa tcaaaaacag cgaagctggc gttgatgttc
     1441 ataaggccaa agacgct
//
LOCUS       DAZPEP010000339         1426 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZPEP010000339 DAZPEP010000000
VERSION     DAZPEP010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1426
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.341"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUT9_004536"
     CDS             complement(<1..118)
                     /gene="rutC"
                     /locus_tag="ACHUT9_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFR1350812.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUT9_004537"
     CDS             complement(130..822)
                     /gene="rutB"
                     /locus_tag="ACHUT9_004537"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFR1350813.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(822..>1426)
                     /locus_tag="ACHUT9_004538"
     CDS             complement(822..>1426)
                     /locus_tag="ACHUT9_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFR1350814.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          397 c          396 g          310 t
ORIGIN      
        1 tatgttgatc aaaagccagc gtaccggaga catacaccac gccatcagcc agcgtgccgg
       61 gaacgaaggg ggccagcggt gcgctgctgc cagcgggaat aattacggat tttggcatcg
      121 ttaaactcct taagcgatac gagcaaagga cgtgggagaa agcgcgtcgc agaacgtttc
      181 gacgtcgctg acccagccaa aaaaggtttc gatattgaac aacgcggctt tctgtgcaaa
      241 ttcaggcccc gcctggtgtg ttgcgtcttc cagcaccacg ccgaaatact ccagaaaaaa
      301 gccatcgcgt agcgtcgatt cgacgcagac gttggtagcg ataccggtga aaaccagatg
      361 gcgtattcca cggctgcgca aaatgctgtc cagcggcgta ttgaagaaac cgctgtagcg
      421 cggcttcggc agcacaatat cgccaggctg cggcaccagt tcatccacca gttgataatc
      481 ccaggagcct ttcgccagca atttcccctg cagctgcggc tgcttacgca tggttttcag
      541 ggcgttcgat ttatgaaaat tcggtgagcc gggtccgcca gcttcgacat actgttcatc
      601 ccagccattt tgaaaccaga tgatcagcat ccctgccgct cgcgctgcgg tcacggcggt
      661 ttgaatgttg gcaatgaccg ggcgagtggt tgagacatca aacccggcga gatctaagta
      721 gccgcctggc gtggcataag cgttttgcat atccaccacg atcagcgcac tttgctgcgg
      781 atcgaaggta atggcttccg gtcgagcggt taaggtcgtc atcatgccac ctcctgagtc
      841 agcgcaggga gatgggcgcg gcactgcatc agtggttgaa tgcgctcgcc gaaggtttcg
      901 attcccgaca gaaaatcatc gaaggtcaac agcacgcctt cggcaccagg cacgcttgcg
      961 acttcatcta acatgcgcgc gacactggcg taagaaccga ctaatgtccc catattgatg
     1021 tttaccgccg aagtgggatc ggccatctga cggacgttgg tgtcagtacc ggagcgggta
     1081 tctttctgac tttgttcggt tagccagctt aacgcctctt catccgcgcc cgctttgtag
     1141 tgttcccatt tggcgcgagc ggcatcgtcg gtttcatcgg caatcaccat aaacaacaca
     1201 taagagccaa cgtcgcgccc ggtttgctct gcggcctgtt tcatccgcac agcggtcggg
     1261 gcgaaagccg tgggtgtatt tacgcctttg ccgaaacaga agttgaaatc ggcatactga
     1321 gcggagaacg ccatgccagc gtcgctttgc ccggcgcaga tcactttcat ggggacactc
     1381 ggttgcggac tgacgcgaca atcattcatg gtgaaaaaat cgcctt
//
LOCUS       DAZPEP010000340         1419 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZPEP010000340 DAZPEP010000000
VERSION     DAZPEP010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.342"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            123..614
                     /locus_tag="ACHUT9_004539"
     CDS             123..614
                     /locus_tag="ACHUT9_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1350815.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            949..1404
                     /gene="ygeH"
                     /locus_tag="ACHUT9_004540"
                     /pseudo
     CDS             949..1404
                     /gene="ygeH"
                     /locus_tag="ACHUT9_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
BASE COUNT          531 a          219 c          230 g          439 t
ORIGIN      
        1 tgaaaaatat accataagca atgcaagctt aaaactaata atttaaaaaa gataaatgta
       61 aaaatattaa tgagtcaatc aataataatt tagcatcacg agacacatca cagaggaata
      121 ttatgagcac agaaacaatt gaaatattca ataatagtga tgaatgggca aatcaactaa
      181 aacacgcatt atcgaaagga gaaaatctgg cattactaca tggtttaact cctgatatcc
      241 ttgatagaat atatgcatat gcattcgact accatgaaaa gggtaatata acggacgcag
      301 aaatttatta taaatttttg tgcatttatg cgttcgaaaa tcatgagtat ctaaaagatt
      361 ttgcatcagt atgtcagcca aaaaagaaat atcaacaagc atatgacctt tacaaactaa
      421 gttacaatta ctccccgtat gatgactatt cagttattta tcgtatgggt caatgtcaga
      481 ttggggctaa aaatatcgat aacgcaatgc aatgtttcta tcacattatt aacaattgtg
      541 aggatgatag tgttaagagt aaagcgcagg catatattga actcttaaac gataattcag
      601 aagataatgg ctaagcatta caatcatttg gttaaatttg ctggttatag tgcagttaac
      661 tactataacc agcacctctt atgttttctt aaatcagcat cttaaaacag ttaagagcat
      721 actataaatc attatttttc aatgcgttag agaacaatct actttataaa aattgaataa
      781 ggctatttca gattacatca ctgaattcct aagataaatt agtgaacaca accatagata
      841 tgtcgaatgt attttcctgt agcattaatg acacataaat atgaatagcc ataatttcta
      901 ttgcttaagc aggatgcaag aaaccaattt tttcatagag gttaactaat ggacttagaa
      961 aataaattct catatcattt tcttgaggga ttaacgctca cggaagatgg aattcttact
     1021 caaggaaatg agcaagttta tattccacag aaagagttag gtgtattaat agtattactt
     1081 gaatccgctg gtcatgttgt actgaaagat atgatcatcg aatcagtatg gaaaaatatt
     1141 attgttagtg acgagtccct gacaagatgt atctattctt tgcgctgcat ttttgaaaaa
     1201 attggctatg atcgttgcat agaaacaatc taccggaaag gttatcgttt cagcgggcag
     1261 gttttcaaaa ctaaaattaa tgaagataat acttcagact attccatagc tatattccct
     1321 ttcactactt cattgaatac actggatcca ttaatactta atcaggaatt agtgcaaatc
     1381 atttcaaata aaaaaatcga tggttagact ggccccctg
//
LOCUS       DAZPEP010000341         1394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZPEP010000341 DAZPEP010000000
VERSION     DAZPEP010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.343"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..459
                     /locus_tag="ACHUT9_004541"
     CDS             <1..459
                     /locus_tag="ACHUT9_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1350816.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="ACHUT9_004542"
     CDS             477..1115
                     /locus_tag="ACHUT9_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFR1350817.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          308 g          360 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac actg
//
LOCUS       DAZPEP010000342         1384 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZPEP010000342 DAZPEP010000000
VERSION     DAZPEP010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.344"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1339)
                     /locus_tag="ACHUT9_004543"
     CDS             complement(<1..1339)
                     /locus_tag="ACHUT9_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFR1350818.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQAR
                     QRLGSEAVRRVFTKTAQLWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTH
                     AGNPALYPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGY
                     YSLGLLNAWSLAGEHRHWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGN
                     EVTARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSR
                     LTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLM
                     TLQGASPGRIPELMRDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          281 a          391 c          357 g          355 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctc gtcggtaatt tcacaagttg tcccatactt gcaagatcgc
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcactt catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgagctctt
      601 catattgcgc tccctttctg agaggtatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttatccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cagtgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZPEP010000343         1377 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZPEP010000343 DAZPEP010000000
VERSION     DAZPEP010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.345"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..984
                     /locus_tag="ACHUT9_004544"
     CDS             <1..984
                     /locus_tag="ACHUT9_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1350819.1"
                     /translation="LVVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGMGEPLPVLYFYHSFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            999..>1377
                     /locus_tag="ACHUT9_004545"
     CDS             999..>1377
                     /locus_tag="ACHUT9_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFR1350820.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          360 a          315 c          362 g          340 t
ORIGIN      
        1 ttagtggtgg gtgttccggt tccggacaat attcacccga tgatcaaaga cgaagcagga
       61 gtctggtcgt ggcgcacgcc tgtcctgaaa ggcaacctgt atgagtattt tttcaatgtt
      121 gatggtgtac gcagcattga tataggcacc gcaatgacta agcctcagcg ccaggttaac
      181 tccagtatgg ttctggtgcc aggcagttat ctggatacgc gttctgttgc gcatggtgat
      241 ttgatcgcca taacttacca ctccaacgca ttgcaatctg aacgtcagat gtatgtctgg
      301 accccgccag gatacaccgg catgggcgag cctttgccag tgctctattt ctatcacagt
      361 tttggtgata ccggacgttc cgctatcgat caggggcgta tcccgcaaat catggataac
      421 ctgctggctg aagggaaaat taaaccgatg ctggtggtga tcccggatac cgaaaccgat
      481 gcgaagggca ttattcccga agatttcgtg cctcaggaaa gacgtaaagt cttttatccg
      541 ctgaatgcta aagcggcaga tcgcgaactg atgaacgata ttatcccgct gattagcaag
      601 cgttttaatg tccgtaaaga tgccgatggt cgcgcgctgg cagggctttc acaaggcggg
      661 taccaggcgc tggtttccgg aatgaatcat ctggaaagct ttggctggct ggccacattc
      721 agtggtgtta ccacgacaac cgtaccggat gaaggtgtcg cggcccggct gaacgatccg
      781 gcagctatta accagcaact acgtaatttt actgtggtcg ttggtgataa agatgtcgta
      841 accggcaagg atatcgccgg gctgaaaact gagcttgagc agaaaaaaat taagtttgat
      901 taccaggaat atcccggcct gaaccatgaa atggatgtct ggcggcctgc ctatgcagcc
      961 ttcgtacaga aattatttaa ataaaataag gcattaagat gaaattaatc attaccgaag
     1021 attaccagga aatgagccgt gtcgcggcac accatctgct tggttatatg tcgaagacgc
     1081 gtcgtgttaa cctggcaatt accgccggta gcacgcccaa aggcatgtat gaatacctca
     1141 ccaccctggt taaaggtaag ccctggtacg ataactgcta tttctataat ttcgatgaaa
     1201 ttccctttcg cggcaaagag ggagaaggcg taacgattac caatctgcgt aatctgtttt
     1261 tcacccctgc ggggatcaaa gaagagaata tccagaagct cactattgat aactaccgcg
     1321 agcatgatca gaaactggcg cgtgaaggcg gcctggattt ggtggtgctg ggattag
//
LOCUS       DAZPEP010000344         1366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZPEP010000344 DAZPEP010000000
VERSION     DAZPEP010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.346"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..1261)
                     /locus_tag="ACHUT9_004546"
     CDS             complement(<1..1261)
                     /locus_tag="ACHUT9_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001493460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFR1350821.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQSSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYT"
BASE COUNT          381 a          348 c          305 g          332 t
ORIGIN      
        1 aggtgtaacc tattgcgttg tttgtattac ggtattcggc aacgacttta atcattccct
       61 ccatcatact tgccacttct gtttcctgcg gcgagatcat gcggacattt ttcatgacct
      121 gggattgcat caccgtctgg ctaccagagt cttccgggcg ttgccaggtc tggatctcct
      181 gatcgttacc gccaacagta cgccagttgg taattgcacc actgaagatg tcacgcacct
      241 gttgttcagt cagggaatta accggattat ccgcattgac gatgaaaaca aacgcttcac
      301 gggcaaaagg cgtgtacagc aaagtaacgc ccgattcctc agcgcgtttt ttctgcccac
      361 cggaagattg cgccacaaaa ataatatcag catcaccttt aacaatcctg ttatacgcat
      421 cgggggtacg agagctctcc agatattccc tggtgtgaaa atcttccggt ataacgctta
      481 aagcataaaa tgcagaggca taaattgggt acgccgccgt agcaccatcg agtcgcggcc
      541 agttttgcgt aaactgaatt tgcggtttac cacgcagtgg tgtcagttga ttattgagtt
      601 tatcgggtcg ccaggcccag gtatcaatat tttcgcgcac accgacaccg ggattgagct
      661 gtttatataa ataaccctga taaccagcga caaaaccaag catgagaagg ataaacagga
      721 gggaattacg gcaacgtagc ccggatgtac cggttaaagg atgtctggtt ggccggccgt
      781 accccagcga aaaactgata tgcgcgccaa cgggtataat gactatccac cagtactcac
      841 ctccgactac tgagactaac aaaacgccca gccaggggag cataagatca gccaaccaac
      901 ccgatcccat ctggtgaaat tgataatctg ccagatgcag cgcgattatc cagccaacca
      961 ggattaataa accgggtact aaaaaaagac attgtcgcca gacggctatt tctctttttt
     1021 ctggtgcact atcgcctgcc catactcgcc ccgagaaaaa tagcattaat gcgcccagcg
     1081 ccagcataaa aatgttgagc agaaggtaac ttgttggcgt gataaggata ttacaaactt
     1141 caataatacc ggcaagtccg acacccagca tagcaacaac cgccgccaaa aattgcgtca
     1201 gtatggggat gccgaaaaaa gtcattacca gcgaggacaa aatccatttt ctgttttgca
     1261 ttattctttc cattcttttt gaatggtgaa attatactcc ccgagtcccc ttgccccttc
     1321 tggacacttt tccgaaatga tggcggaaaa aaacgggacc ctttgg
//
LOCUS       DAZPEP010000345         1341 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZPEP010000345 DAZPEP010000000
VERSION     DAZPEP010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.347"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..184
                     /locus_tag="ACHUT9_004547"
     CDS             <1..184
                     /locus_tag="ACHUT9_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350822.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUT9_004548"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="ACHUT9_004548"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFR1350823.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAZPEP010000346         1320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZPEP010000346 DAZPEP010000000
VERSION     DAZPEP010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.348"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            142..813
                     /gene="queE"
                     /locus_tag="ACHUT9_004549"
     CDS             142..813
                     /gene="queE"
                     /locus_tag="ACHUT9_004549"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFR1350824.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          374 a          266 c          290 g          390 t
ORIGIN      
        1 cataatacgt tactcccttt aattattaac gatatgtttc gcatttttct ttaaatctgc
       61 ctgtaacaga atgttaataa gacatgattg ttgtggcagg atctgcaata attaacaatt
      121 atccacttca cagagaatgc tatgcagtac ccgattaacg agatgttcca gaccctgcaa
      181 ggtgagggtt actttaccgg cgtccccgcc atttttattc gtttacaggg atgcccggtt
      241 ggctgtgcct ggtgcgacac caaacacacc tgggaaaagc ttgaggatcg ggaagtctcc
      301 cttttcagca ttctggcgaa gaccaaagag agtgataagt ggggggcggc gagcagtgaa
      361 gacttgttgg ctgtcattag tcgccaggga tacaccgcgc ggcatgtggt gattacgggt
      421 ggtgagcctt gcattcatga tttgctacca ctgactgatc tgctcgaaaa gaacggtttt
      481 agctgccaga tcgaaaccag tggtactcat gaggtacgct gcacaccgaa tacctgggtt
      541 accgtatcgc caaagctgaa catgcgcggc ggctatgacg tgttgtcaca ggcactggag
      601 cgagccaacg aaatcaagca tccagtgggg cgcgtacgcg atattgaagc actggatgaa
      661 ctattggcaa cgctgaccga tgataaaccg cgagtcattg cactgcagcc gattagccaa
      721 aaggatgatg ccacacgttt gtgcattgaa acctgcattg cgcgtaattg gcgtttgtcg
      781 atgcaaacac ataaatatct aaatattgcc tgattaaaca tttataagcg ttataaatgg
      841 gtggaacctg taaagacttc tactcattta tattgtttgt cgcctctgaa aactcctcca
      901 ttttacccat ccagggctaa tcattagcat tctctacaaa ttctgtggca ttaatttttc
      961 gctggagtga aaattattgc ggtaaagttt ggtagatttt aatttgtata gagttatttt
     1021 aaatatttac ctttttaatc aatgaattaa gtactcttta acataatgga tgtgttgttt
     1081 gtgtgatact gtaaagttgg tagattgtga ctggcttaaa aaatcattaa ttaataatag
     1141 gttatgttta gagtgttccc cgcgccagcg gggataaacc accatataac ccgttatctc
     1201 tttctcaagt ttttatatta gcagtacttg taataagcaa aatatccacg taacacctca
     1261 tgttcaaaat agttctccat gccagagagg ttcacaatta tcgatacaaa aaattaaatt
//
LOCUS       DAZPEP010000347         1320 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZPEP010000347 DAZPEP010000000
VERSION     DAZPEP010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.349"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..346)
                     /locus_tag="ACHUT9_004550"
     CDS             complement(<1..346)
                     /locus_tag="ACHUT9_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350825.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERA"
     gene            complement(349..1059)
                     /locus_tag="ACHUT9_004551"
     CDS             complement(349..1059)
                     /locus_tag="ACHUT9_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350826.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1075..>1320)
                     /locus_tag="ACHUT9_004552"
     CDS             complement(1075..>1320)
                     /locus_tag="ACHUT9_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1350827.1"
                     /translation="FRYYATQMNADKNIKLLAINGIAPTAENIRNGKYPYIVDAFMVT
                     RENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT          351 a          317 c          293 g          359 t
ORIGIN      
        1 ttgcccgttc atccgcacgc cagttttctt tgttggcaag gttgatacgg cgcagagaaa
       61 aaactacgtg aatattgtca aaatgcccct gtgcaacaaa ttccagtaaa cgcggcagaa
      121 aatagcggac ctcatcgcta ttttgcggat caggccctgg gctactattc caggcattga
      181 ttaaggaata cgggatcgct cgtaacgatg tactccgtaa tgccttttgt tcctgctgac
      241 tcaggcaata ttcacaacaa accacaaatt tctgcggcac ccgataacga cgaaaggtgt
      301 gataaatctg ctcaattaat gcctgcatgg cggggctggg ttgcatagtt accccctcgc
      361 ccgctgatcg cgtgtaaacg gttgatagac aaaacgagca ttcgccgcgt tcaggcgaat
      421 aagggcgcta cgcgaaaggg aaaagctggg gcgaatgcaa ctattgcagt ctcgacagct
      481 ttcctctgcg caattttcca ctgagtaatg acgcgtgtag agtttcagtt cggcctgctt
      541 atcgcgaaat ccgcgtaaac tgttattgtt atgaaatttc caggcatctt taaagacctg
      601 aaagatatag acatcctcaa tccaggagtc acatttttta agcgggcgct ttgctattat
      661 cacccgctca tccggattta gcatcgccgt aaattgtacg gtgacgtcat ttgtcgtgtt
      721 caggtcacat tgaacatctg gtacatcact gatatagggg tcaaatccaa aatcagcacc
      781 acaggcacaa agacggctaa tggtggcggt atcgagggaa aatcccggcc ataccttgct
      841 ggaggttaag ccaatattta atgtgacatc atatcgaagc aatccctcaa gaccgtcagc
      901 accgacgaac ttttcccctt catccaaggc ctggttaata agttgctgaa caatgccttc
      961 atcccagtat gccgatttgc agttggcaat cgtaatatcc acgcctgtga agggatcagt
     1021 accggcaagg cttgcaaata atgtattaac taattccatt tttattatcc agctttaagg
     1081 tagcgtctta tacatcggga cataacctac atcttccacc aaactctgtc cctgcggcgt
     1141 taaaaaccac tccaacaatt tttgtgtctc tgacgtcgta ttttcccgcg ttaccataaa
     1201 ggcatcgacg atatagggat atttgccgtt gcgaatgttt tcggcagtcg gtgcaatacc
     1261 gttaatcgct agcagtttta tatttttatc ggcgttcatt tgtgttgcgt aataacggaa
//
LOCUS       DAZPEP010000348         1302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZPEP010000348 DAZPEP010000000
VERSION     DAZPEP010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.350"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            102..1076
                     /gene="cysB"
                     /locus_tag="ACHUT9_004553"
     CDS             102..1076
                     /gene="cysB"
                     /locus_tag="ACHUT9_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFR1350828.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          342 a          300 c          294 g          366 t
ORIGIN      
        1 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
       61 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
      121 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
      181 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
      241 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcaggg caagaaataa
      301 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggagagc
      361 acacctggcc ggataaaggc tcactgtata tcgccaccac gcatacccag gcacgctacg
      421 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
      481 accagggctc gccgacacaa attgctgatg ccgtctctaa aggcaatgct gatttcgcta
      541 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
      601 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
      661 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
      721 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg
      781 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
      841 cggtggatcc ggtcgccgat cccgaccttg tgcgtgttga tgctcacgat atcttcagcc
      901 acagtacaac caaaattggt tttcgccgta gtactttctt gcgcagttat atgtatgatt
      961 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
     1021 gctctaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
     1081 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tcttttgtta
     1141 cttatttcta taccgtgcac aatattttta tccctcatat ttatagggta aattacctgt
     1201 aaagtagtgt ctaattaccc gcctcgcaaa ctccagtttt caaatatcta tttcaggtca
     1261 aaagattgaa ttattcactg gagacgattc gtaaattcgc tg
//
LOCUS       DAZPEP010000349         1299 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZPEP010000349 DAZPEP010000000
VERSION     DAZPEP010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.351"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..1117
                     /locus_tag="ACHUT9_004554"
     CDS             <1..1117
                     /locus_tag="ACHUT9_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1350829.1"
                     /translation="TWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELAVMLGRLERELRAGAVSAESEAWLAQCGLTVEQMES
                     QMEAEYIPERRLHLYHCDHRGLPQALISPEGETAWRGEYDEWGNQLNEENPHHLYQPY
                     RLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGINLYTYPLAPIRYTDPLGL
                     ERVISVYGPPAPDRAGAETPLVLTDMTGGVTIYYDPETGDSMTFDSSNRIDRRSQRGA
                     GDPYTGEVVGCETNGSGISAAYGTTKIYTTDTRARWLHGGGSSLRDPYAPRQGWKPTM
                     GCTRAQNEDVDELCKKVTSWMYSHPGERIRYERFKTR"
     gene            1095..>1299
                     /locus_tag="ACHUT9_004555"
     CDS             1095..>1299
                     /locus_tag="ACHUT9_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350830.1"
                     /translation="MNVLRHVNAINMGVCILILVIGCANAKVLKRCILPAPDGGVEKL
                     SKPNLHGVIKKVDLNTNEAEIKIK"
BASE COUNT          356 a          263 c          400 g          280 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacact gatccactgg ggttagaacg
      661 agttataagt gtatatgggc ctcccgctcc agaccgggca ggtgctgaaa ctcctcttgt
      721 tcttactgat atgactggag gagtgactat ttattatgat cctgaaacag gtgattcaat
      781 gacttttgat agtagcaata gaattgatcg tcgttctcaa cgtggtgctg gtgatccata
      841 tacaggtgaa gtcgttggtt gtgagacaaa tggatcagga atatcggcag catatggcac
      901 aacgaagatc tatactacag atactagagc aagatggcta catggtgggg gatcatcgtt
      961 gcgcgatcct tatgctcctc gacaaggctg gaaaccaact atgggatgta cccgtgcaca
     1021 gaatgaagat gtagatgagt tgtgtaagaa agtgacaagt tggatgtatt cgcatcctgg
     1081 agagagaatt agatatgaac gttttaagac acgttaatgc gattaacatg ggagtgtgca
     1141 ttctaatttt ggttattgga tgtgcaaatg ctaaagttct taaacgatgt atattacccg
     1201 ctcctgatgg tggcgtagaa aaactatcaa aacccaactt acatggagta attaaaaaag
     1261 ttgatttgaa cacaaatgaa gcggaaatta aaataaaag
//
LOCUS       DAZPEP010000350         1286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZPEP010000350 DAZPEP010000000
VERSION     DAZPEP010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.352"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            255..773
                     /locus_tag="ACHUT9_004556"
     CDS             255..773
                     /locus_tag="ACHUT9_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFR1350831.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            983..>1286
                     /locus_tag="ACHUT9_004557"
     CDS             983..>1286
                     /locus_tag="ACHUT9_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFR1350832.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWR"
BASE COUNT          317 a          314 c          330 g          325 t
ORIGIN      
        1 cattttatgg aggcaatacg gactttgaac agttgggtat ggttatccag atatatatta
       61 ttaagttatt gctgaataag agatttaatg attttaaaaa tagtttttac gacaaccgta
      121 aattctagct cggtttgtat gtaaaaaata ctgaaaaatg atgcggttcc gtgaagtgag
      181 ctgcatctgc ctgtctggga ttaacggcag gtcattgtaa gtagtaatta accacgttaa
      241 caaggagttt acaaatgcca acaccgtgtt atatctccat cactggtcag acccagggaa
      301 atattactgc aggtgcattt accgctgatt ctgtcggcaa catctatgtg caggggcacg
      361 aagacgagat gctggtgcag gaattcctgc ataacgttac tgtcccgacc actcctcagt
      421 ccggtcagcc tgcgggtcag cgtgctcaca agcctttcat ttttaccgtg gcgctgaaca
      481 aagcggttcc gctgatgtac aacgcactgg cctccggcga aatgctgcca accactgaac
      541 ttcactggtg gcgtacctct gtggagggca aacaggagca ttatttcact acccgcctga
      601 ctgattcgac gattgtcgat atgaaactgc atatgccgca ttgccaggac ccggcgaaac
      661 gcgagttcac ccagttgctc gaggtttcac tggcctaccg caaaattgaa tgggaacacg
      721 tcaaatctgg cacctccggt gccgacgact ggcgcgcacc actggaagca taagttaagc
      781 caacagcatc cggctatcag gccggatgct gtttttgtat tacgccgtag aaagaaaggt
      841 tgtcagtaag gggttacgtt cacgggagat ggcggatgcc ggaaacttcg gcggtagcgt
      901 gcctgaacgc aagcgtaaca ctgtactgtc acagccgctt taacagcgaa ctgtctgttt
      961 ttccttaacc ccggaggttg ctatgtcaac cggattacgt ttcacgctgg aagtggacgg
     1021 cctgccaccg gatgcttttg cggtggtctc ctttcatctg aaccagtcac tctcttcgct
     1081 cttttccctc gagctctctc tggtcagcca gcagtttctc tcccttgaat ttccgcaggt
     1141 actggacaaa atggcctacc tgacgatatg gcagggcgat gacgtacagc gccgggtgaa
     1201 aggtgtggtg acctggtttg aactggggga gaacgacaaa aaccagatgc tgtacagcat
     1261 gaaggtatgc ccaccgctgt ggcgca
//
LOCUS       DAZPEP010000351         1253 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZPEP010000351 DAZPEP010000000
VERSION     DAZPEP010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.353"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            212..1087
                     /gene="nadA"
                     /locus_tag="ACHUT9_004558"
                     /pseudo
     CDS             212..1087
                     /gene="nadA"
                     /locus_tag="ACHUT9_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
BASE COUNT          340 a          299 c          305 g          309 t
ORIGIN      
        1 caggcatcct caatttcact ttgtaaacct gatgacatcg tcagagctta ctgtgcaagc
       61 aactctacgt cggtggaatt aggcgtaatt cgagtgactt ttccccacca ttcgactatc
      121 ttgtttagca tataaaacaa attacgccga tagtagcgaa tattacgcta atgtcggttt
      181 taacgttaag cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc
      241 gatttatcct ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg
      301 cgagaagata aaacgtctgc taaaagaacg taatgcggtg atggttgccc actactatac
      361 cgaccccgaa attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga
      421 aatggcgcgc ttcggtgcaa agcatcccgc ttctacgttg ttagtcgctg gggtgagatt
      481 tatgggagaa accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc tgacacttca
      541 ggctgaatgt tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc
      601 ccatcccgat catactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc
      661 agattgggtg gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagttttgg
      721 tgaaaaaatc atctgggcac ccgacaaaca tctggggcgt tacgtgcaaa aacagacggg
      781 cgcagacatt ttatgctggc agggtgcctg tattgtgcat gatgaattta agactcaggc
      841 gttaacccgc ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc
      901 acaagctatt gtcgatatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc
      961 gaaaacattg ccacatcagc ggcttatcgt ggcaaccgat cggggcattt tctacaaaat
     1021 gcagcaggcg gtgccagata aagagttact ggaagcacca accgcaggtg agggtgcaac
     1081 ctgccgcagg tgtcagcgcc agtgatataa gacggtaatt cgccatttgg attgtccgct
     1141 ccacccaaca tgttgtttcc ttaaggttct cacaccagaa aggacatcaa catgctgagc
     1201 agagaggact tttacatgat aaagcaaatg cgccagcagg gcgcgtacat cgt
//
LOCUS       DAZPEP010000352         1240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZPEP010000352 DAZPEP010000000
VERSION     DAZPEP010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.354"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            116..799
                     /gene="tssD"
                     /locus_tag="ACHUT9_004559"
                     /pseudo
     CDS             116..799
                     /gene="tssD"
                     /locus_tag="ACHUT9_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            779..>1240
                     /locus_tag="ACHUT9_004560"
     CDS             779..>1240
                     /locus_tag="ACHUT9_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350833.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPELAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTGFAPIFPDICYHLTHYKPAA"
BASE COUNT          385 a          243 c          266 g          346 t
ORIGIN      
        1 gtgaaacatt ttgtttttca cattttagtt catcactttt aggcgtaaat aaaggtaaaa
       61 aaaacaacaa tactatttct ctttcacaaa agaattaaca aaaggttttt caaggatgag
      121 tgatattatt tacctgacca taaccggaga acaacaagga tgtatttcct ctcgttgtgg
      181 aacgagcgca tcaataggta atcgctggca gattggccat gatgatgaaa tcttcgcgtt
      241 ctcgctctct aatagtatta cgaatacagg caaagggtca taattgcatg gccttagttt
      301 ctgtaaattg attgacaaaa gctccccgtt acttattaac gctattaaca ataacgagca
      361 gttgttcatg gagtttgact tctatcggat caataggttt ggtcgatggg agaaatatta
      421 caacattcaa ctccgcggcg cgctcttatc agcaatcaat cacctgttta ctgagaacaa
      481 tctggataca gaagccatca ctgtcagtta ttaatatata ctctccaggc atctgattgc
      541 taatactgaa tttagttatt gggcatttcc cgataactac aaccgtctgt ttataccacg
      601 gccaaaaact aaagctggta atggtttcaa aacacttaat agtaaagcgg ttggcagact
      661 gcttgctgct gtcggcatat acaatgggaa tatcgaagga ttcagggaaa cagcagacaa
      721 gttgggggga gatgcacctg ctggttatga tcaatttatg gataacaaag ggttactcat
      781 cgctggtgct tcaatagttg ctggagcagt tctggggaga atgagactcc ctgatttgga
      841 atcacttgag catttcggag ccagaggagc agtatcagga agaccattta atcccgagct
      901 tgccggtggt ccaatagaaa acctgacgac agatggcgta acaatcaaca gggaaggtat
      961 cgctattgtt gaaaaacata tagccagatt tggtcatgat cctgtaaatg aagtgatgat
     1021 caatagactg aaagacattg agaaaggaaa aatcccccca gaacaagtag atcttaactt
     1081 ctatactcat gagtgtcggg aatatcagcg ttactgtaat cttggttggg aaacaggaca
     1141 acctgatggt gacgcaggct atgcactctg gaatcatact ggatttgccc ctatatttcc
     1201 agacatctgt tatcacttaa cccattacaa gcccgctgcc
//
LOCUS       DAZPEP010000353         1234 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZPEP010000353 DAZPEP010000000
VERSION     DAZPEP010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.355"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..761)
                     /locus_tag="ACHUT9_004561"
     CDS             complement(<1..761)
                     /locus_tag="ACHUT9_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFR1350834.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     DAKGEKRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTPPTDKI
                     IPGDGRTLVVHFDERGNIRFTETVPDGARVCTMET"
BASE COUNT          307 a          299 c          310 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcgagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aacg
//
LOCUS       DAZPEP010000354         1228 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZPEP010000354 DAZPEP010000000
VERSION     DAZPEP010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.356"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(140..>1228)
                     /gene="ompC"
                     /locus_tag="ACHUT9_004562"
     CDS             complement(140..>1228)
                     /gene="ompC"
                     /locus_tag="ACHUT9_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFR1350835.1"
                     /translation="IVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYFTDDK
                     RDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAGLKIS
                     EFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDFFGLI
                     EGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKSDRTN
                     AQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNKAQSF
                     EVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYVDYKI
                     NLIDESDFTRAVDIRTDNIVATGITYQF"
BASE COUNT          344 a          279 c          268 g          337 t
ORIGIN      
        1 tgtccccgga ggatatggaa taatattaat tgaggtgaat gaccagaaat atcaaaaatt
       61 gctcattttt acttaaggag tcatacagca gcacttaaat caaaaaccag cccagagaag
      121 ggctggtcag gagaggacgt tagaactgat aggtaatgcc cgtagcgacg atgttatcgg
      181 tccgaatatc tacggcacgg gtaaagtcgc tttcatcaat caggttgatt ttataatcaa
      241 cataggtcga catatttttg ttgaagaaat acgtcgcacc aacgtcgata tactcaatca
      301 tgtcctggtc gccgtagcgg cccagatctc tgcctttcga tttcaggtaa gcgagtgatg
      361 ggcgcaagcc gaagtcgaat tgatattgcg ccaccacttc aaaactttgt gctttgttag
      421 agatataacc atccccatag gtggtcatgt ttaatgtttc agcgtaatta gctgcaaagt
      481 agatgttgtt ggcatcatac tttatacctg tggcccatag ttctgcattc ttaccggagg
      541 cattgagagg attagcagcg gctctttcct gcgcatttgt ccgatcggat ttggtatata
      601 ccgcaccgat accaaagcca tcataaacat aagttgagga gataccgaaa ccgtcaccgt
      661 tggcacgcgt gtagtcagca ccataaagat gactgttgtc agtacgttca tttttgcctt
      721 gatattgcgc ggcaaagttc agcccttcaa ttaagccaaa gaaatcgtag ttgcgatagg
      781 ttgctagacc ggtggtacgg tatgtcatga agacatcttt ctgactccag gtatcgccac
      841 caaattctgg caacatatcg gtccatgaac caatgtcgta gccgacaccg tagttacgac
      901 catagtccag agagccaaat tcactaattt ttaaaccagc ataggccaga cgggtcttgt
      961 tgccgcgcga gccgttggct tcatcgttat agcctttaaa atcatattcc cagtgaccaa
     1021 aaccaatcat ttgatcattg atttgcgttt ctcctttaaa gccgagacgg gcataagttt
     1081 tatcaccatc gtcacgctta tcatcagtaa aataacgtag agcggtaacc ttgccataaa
     1141 ggtcgagttt attaccatcc ttgttataga cttcggcagc gtgtgctacg ttcgcagcta
     1201 acaaaccagt tacaaccacc gcaactat
//
LOCUS       DAZPEP010000355         1187 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZPEP010000355 DAZPEP010000000
VERSION     DAZPEP010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.357"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..755)
                     /locus_tag="ACHUT9_004563"
     CDS             complement(<1..755)
                     /locus_tag="ACHUT9_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFR1350836.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTIGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFSEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINMPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELVEHLKKA"
     gene            1071..>1187
                     /locus_tag="ACHUT9_004564"
     CDS             1071..>1187
                     /locus_tag="ACHUT9_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350837.1"
                     /translation="MYQRCFDNASETLFVVGKTPRLSRFAFSDDPKWESGHHV"
BASE COUNT          290 a          286 c          289 g          322 t
ORIGIN      
        1 gctttcttca gatgttccac cagctcggca atggcctccg gcgaatcgtt atcgagaatg
       61 atgtgcttac gttctgtttg cggcggtacg cgaatgccgg tcagttcagc aagtggcgcg
      121 ctctggctcc agccaatatc acttgcctgc cactgattta ccggtttttt acccgcgccg
      181 agaatggctt tcatcgaagg aatacgtggc atgttaatat cggaggtgac gcagagcacg
      241 gctggaacag agagctcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt
      301 gtattgccct gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct
      361 ccgaccagca agccaacctg ctgggcataa aggtcgccgg aaccttcact aaagatcagt
      421 aaatcgaagc cgatcttttc aactgctgcc gccagcgcct ttgcggtatc gagaggcagt
      481 gcatgttcaa gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg
      541 tctttgcgca ctttcgagtt ctgcaacaat gagccaccaa tggtcagcgc ggctatctca
      601 tcgtcatctg ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt
      661 ttggcgtcgg cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct
      721 tcaggcacca gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg
      781 aaccatctcc gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt
      841 tttcgacgga gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa
      901 aacaaaagtg caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt
      961 gtaaataatc ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat
     1021 tttatccggc atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac
     1081 gctgttttga taacgccagt gaaacgctgt ttgtcgtcgg taaaacgcca cggctttcac
     1141 gttttgcatt tagcgatgat ccaaaatggg agtccggaca tcacgtt
//
LOCUS       DAZPEP010000356         1176 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZPEP010000356 DAZPEP010000000
VERSION     DAZPEP010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1176
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.358"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>1176
                     /gene="fimD"
                     /locus_tag="ACHUT9_004565"
     CDS             <1..>1176
                     /gene="fimD"
                     /locus_tag="ACHUT9_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFR1350838.1"
                     /translation="SHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKWQHIN
                     TWLERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVIHGIA
                     RGTAQVTIKQNGYDIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFTVPYS
                     SVPLLQREGHTRYSITAGEYRSGNAQQEKTRFFQSTLLHGLPAGWTIYGGTQLADRYR
                     AFNFGIGKNMGALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQLVGY
                     RYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTVTQQL
                     GRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRDQMLA
                     "
BASE COUNT          332 a          262 c          285 g          297 t
ORIGIN      
        1 agccattatg catatttaaa cctacagagt gggctaaata ttggtgcgtg gcgtttacgc
       61 gacaatacca cctggagtta taacagtagc gacagttcat caggtagcaa aaataaatgg
      121 cagcatatca atacctggct tgagcgagac ataattccgt tacgttcccg gctgacgctg
      181 ggtgatggtt atacccaggg cgatattttc gatggtatta actttcgcgg cgcacaattg
      241 gcctcagatg acaatatgtt acccgatagc caaagaggat ttgccccggt gatccacggt
      301 attgctcgtg gtactgcaca ggtcactatt aaacaaaatg ggtatgacat ttataatagt
      361 acggtgccgc cggggccttt taccatcaac gatatctatg ccgcaggtaa tagtggtgac
      421 ttgcaggtaa cgattaaaga ggctgacggc agcacgcaga tctttaccgt accctattcg
      481 tcagtcccgc ttttgcaacg tgaagggcat actcgttatt ccattacggc aggagaatac
      541 cgtagtggaa atgcgcagca ggaaaaaacc cgctttttcc agagtacatt actccacggc
      601 cttccggctg gctggacaat atatggtgga acgcaactgg cggatcgtta tcgtgcgttt
      661 aatttcggta tcgggaaaaa catgggggca ctgggcgctc tgtctgtgga tatgacgcag
      721 gctaattcca cacttcccga tgacagtcag catgacggac aatcggtgcg ttttctctat
      781 aacaaatcgc tcaatgagtc aggcacgaat attcagttag tgggttaccg ttattcgacc
      841 agtggatatt ttaatttcgc tgatacaaca tacagtcgaa tgaatggcta caacatcgaa
      901 acacaggatg gtgttattca ggttaagccg aaattcaccg actattacaa tctcgcttat
      961 aacaaacgcg ggaaattaca actcaccgtt actcagcaac tcgggcgcac atcaacactg
     1021 tatttgagtg gtagccatca aacttattgg ggaacgagta atgtcgatga gcaattccag
     1081 gctggtttaa atactgcatt cgaagatatc aactggacgc tcagctatag cctgacgaaa
     1141 aacgcctggc aaaaaggacg ggatcagatg ttagcg
//
LOCUS       DAZPEP010000357         1166 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZPEP010000357 DAZPEP010000000
VERSION     DAZPEP010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.359"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            196..>1166
                     /gene="cadB"
                     /locus_tag="ACHUT9_004566"
     CDS             196..>1166
                     /gene="cadB"
                     /locus_tag="ACHUT9_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFR1350839.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT          256 a          253 c          287 g          370 t
ORIGIN      
        1 atatcaactt gcgatcctga tgtgttaata aaaaacctca agttctcact tacagaaact
       61 tttgtgttat ttcacctaat ctttaggatt aatccttttt tcgtgagtaa tcttatcgcc
      121 agtttggtct ggtcaggaaa tagttataca tcatgacccg gactccaaat tcaaaaatga
      181 aattaggaga agagcatgag ttctgccaag aagatcgggc tatttgcctg taccggtgtt
      241 gttgccggta atatgatggg gagcggtatt gcattattac ctgcgaacct agcaagtatc
      301 ggtggtattg ctatctgggg ttggattatc tctattattg gtgcaatgtc gctggcgtat
      361 gtatatgccc gactggcaac aaaaaacccg caacaaggtg gcccaattgc ttatgccgga
      421 gaaatttctc ctgcatttgg ttttcagaca ggtgttcttt attaccatgc taactggatt
      481 ggtaacctag cgattggtat taccgctgta tcttatcttt ccaccttctt cccagtatta
      541 aatgatcctg ttccggcggg tatcgcctgt attgctatcg tctgggtatt tacctttgta
      601 aatatgctcg gcggtacctg ggtaagccgt ttaaccacta ttggtctggt gctggttctt
      661 attcctgtgg tgatgactgc tattgttggc tggcattggt ttgatgcggc aacttatgca
      721 gctaactgga atactgcgga taccactgat ggtcatgcga tcattaaaag tattctgctc
      781 tacctgtggg ccttcgtggg tgttgagtcc gcagcagtaa gtactggtat ggttaaaaac
      841 ccgaaacgta ccgttccgct ggcaaccatg ctgggtactg gtttagcagg tattgtttac
      901 atcgctgcga ctcaggtgct ttccggtatg tatccgtctt ctgtaatggc ggcttccggt
      961 gctccgtttg caatcagtgc ttcaactatc ctcggtaact gggctgcgcc gctggtttct
     1021 gcattcaccg cctttgcgtg cctgacttct ctgggctcct ggatgatgtt ggtaggccag
     1081 gcaggtgtac gtgccgctaa cgacggtaac ttcccgaaag tttatggtga agtcgacagc
     1141 aacggtattc cgaaaaaagg tctgct
//
LOCUS       DAZPEP010000358         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZPEP010000358 DAZPEP010000000
VERSION     DAZPEP010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.360"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            248..493
                     /gene="yahM"
                     /locus_tag="ACHUT9_004567"
     CDS             248..493
                     /gene="yahM"
                     /locus_tag="ACHUT9_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFR1350840.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(510..>1162)
                     /locus_tag="ACHUT9_004568"
     CDS             complement(510..>1162)
                     /locus_tag="ACHUT9_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFR1350841.1"
                     /translation="FMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRR
                     AGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQS
                     TPQMSTLQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWA
                     GIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYEGVTQR"
BASE COUNT          352 a          277 c          275 g          258 t
ORIGIN      
        1 gtttcgcagc catgcggtgt ataaaaaatg atctcatgca gatgttttgt gaatgtgtgg
       61 gttgacattc atatgaaaaa aatcataatt ccatcatgtt tgtgtggggt cttttctgta
      121 ccttacgcat cgcactcaag cctgacagaa aatatgctgt aaggctcata tcaaaaaccg
      181 ccattagctc atcaggaaga gcagacgaca accaggattg ttgtatggta cggggttcga
      241 ggcctcgatg gcggtccaac ttttcaaaac tcttctgaat cagatcccct tattatcatc
      301 cctgcaaagt ggaacattgc cattgtttgg ctattcagga cgggtcagac caatgaaaaa
      361 agcccacaca ggggagagtg ggctgaaatg ggaagctaaa gactcaagta aacttatcgg
      421 aaataaggac cacgcattac gggggctatc atcgccgata gtcgggattc ggcaaatcag
      481 gttgattacg tagagagtaa attatctgct caccgctgcg tcaccccttc gtaaatcagg
      541 cgtagcgcga atacaccaat aattgcacca ataacccgac tggcaacgcg ttgcatacgc
      601 ccataagcac gacgcacagc gggcaaagaa aacgcctgac taagaaagac tcgccagata
      661 actgatgcga gcacaatccc cgcccaggcc attaaacgtg cccatgttgg tgtttcggca
      721 tttaatgtta ctgagaaaat actgataaaa aataaaacgg tttgcgggtt agagagatcg
      781 gtaattaatc cgcggcgaaa aaagacatac caggaggcgc taatcggttg ttgtagtgtg
      841 ctcatttgcg gtgttgactg gcggcgcatg ctgcaccacg caaaccataa gagataagcg
      901 ccgccgacga ttctgataag cgaaaaaatc tcctcacact gcgttattag cgttgcaaga
      961 ccaaacaaac ccaacccgga ataaaatgca tcgcccagcg ccacgcccag tccggtcagc
     1021 acccctgcgc gtcgaccgga agccaggctg gtttgtacta ccacaaagag attggctccc
     1081 ggattaaaaa aagtaatcac gaacagtcct acggtcaggt aaacggcgtg caaaggatcc
     1141 atagtgattt catccataaa ta
//
LOCUS       DAZPEP010000359         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZPEP010000359 DAZPEP010000000
VERSION     DAZPEP010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.361"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..365)
                     /locus_tag="ACHUT9_004569"
     CDS             complement(<1..365)
                     /locus_tag="ACHUT9_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1350842.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVISASPDVA"
     gene            complement(347..769)
                     /locus_tag="ACHUT9_004570"
     CDS             complement(347..769)
                     /locus_tag="ACHUT9_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1350843.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLFLMKRQAEQADSDSN
                     QQVALADLIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPAEAISHAEN
                     VVYRLSGMYEFVVNDAPEQTEDAGPAEPVSAGKCSTVS"
     gene            complement(785..>1162)
                     /locus_tag="ACHUT9_004571"
     CDS             complement(785..>1162)
                     /locus_tag="ACHUT9_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350844.1"
                     /translation="GWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTAATGMT
                     VTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKGVAAGK
                     VNIPVVSGNGEFAAVAEINVTDS"
BASE COUNT          246 a          355 c          295 g          266 t
ORIGIN      
        1 tcagccacat ccggggaagc ggagataact tcattcccgt ccggaccaaa gcggacgcct
       61 ccggcaagcc ctgccgcttt ctgcatcagc acatcatctt caggctcttc gtcagcctcg
      121 cgccggttca gcagactgaa atccagcgga tgcatatccg gatcgctgaa aaacaggctg
      181 agtacggtgt acgtcagccc ggaaaagtgc atatccagca gaacatcatg aaaataatgg
      241 gtactgtaaa agcggtgcca gtcggcgtac tccgtggatg acatcccggc aagcatggcg
      301 cgccagtcgg gtcgccccat ctctcgcgcc agtttcaggg caaaactcag ctcaccgtcg
      361 aacactttcc cgcagaaaca ggctctgcag gcccggcgtc ctctgtctgt tcaggggcat
      421 cattcaccac aaactcatac attccggaca gccggtacac cacgttttca gcatgagaaa
      481 ttgcctccgc gggccaggtg gtaagcactt cctgctcaat ctgtttaacg gcttcattca
      541 tggacggcat ctgcgtcttc ttcggatggt tatgccacag ggacatcgcc accacaaaag
      601 caccggttct gataaggtct gccaaagcaa cctgttggtt gctgtcggag tccgcctgtt
      661 ctgcctgtcg tttcatcaga aaaagatgct caatacgctg cagggctgac agttcagaaa
      721 gcgtgacggt cacgccgtta tgttcaaatg attcggtttt caggaacatc gctgactctc
      781 cggattagct gtcggtgacg ttgatttctg caaccgcagc aaactcacca ttaccggata
      841 caaccggaat gttgaccttg cctgcagcaa cacctttcac ggtgatggtc ataccactga
      901 ccgacacggt ggcttttgtt ttatccgcag acaccgcacg gaagctcttg tcggttgcgc
      961 cctccggctg gaatgccacg gtcagcgtcg tgctctgccc tttcaccacc gaggtgctgg
     1021 caggcgtcac ggtcataccg gttgccgctg ttaccgtgct gcgatcttct gccatcgacg
     1081 gacgtcccac gttggtgact ttcaccgtgc gggtgatcac ttccttcgct gtcaccgcct
     1141 taccgatgct gctgacccag cc
//
LOCUS       DAZPEP010000360         1162 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZPEP010000360 DAZPEP010000000
VERSION     DAZPEP010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.362"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..365)
                     /locus_tag="ACHUT9_004572"
     CDS             complement(<1..365)
                     /locus_tag="ACHUT9_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFR1350845.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVA"
     gene            complement(347..769)
                     /locus_tag="ACHUT9_004573"
     CDS             complement(347..769)
                     /locus_tag="ACHUT9_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFR1350846.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            complement(785..>1162)
                     /locus_tag="ACHUT9_004574"
     CDS             complement(785..>1162)
                     /locus_tag="ACHUT9_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350847.1"
                     /translation="GWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTATTGMT
                     VTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKGVAAGK
                     VNIPVVSGNGEFAAVAEINVTAS"
BASE COUNT          247 a          363 c          291 g          261 t
ORIGIN      
        1 tccgccacat ccggggaagc ggggataact tcattcccgt ccgggccaaa gcggacacct
       61 ccggcaagcc ctgccgcttt ctgcatcagc acatcatctt caggctcttc gtcagcctcg
      121 cgccggttca gcagactgaa atccagcgga tgcatctccg gatcgctgaa aaacaggctg
      181 agcacggtgt acgtcagccc ggaaaagtgc atatccagca gaacatcatg aaaataatgg
      241 gtactgtaaa agcggtgcca gtcggcatac tccgtggatg acatcccggc aagcatggcg
      301 cgccagtcgg gtcgccccat ctcacgcgcc agtttcaggg caaaactcag ctcaccgtcg
      361 aacactttcc cgcagaaaca ggctctgcag gcccggcgtc ctctgcctgt tcaggagcat
      421 cattcaccac aaactcatac ataccggaca gccggtacac cacgttttca gcatgagaaa
      481 ttgcctctgt gggccaggtg gtaagcactt cctgctcaat ctgtttaacg gcttcattca
      541 tggacggcat ctgcgtcttc ttcggatggt tatgccacag ggacatcgca accacaaaag
      601 cgccggttct gatggcgtct tccacagtaa acttccggtt gctgtctgac tccgcctgtt
      661 ctgcctgccg tttcatcagg tcgagatgct caatacgctg cagggctgac agttcagaaa
      721 gcgtgacggt cacaccgtta tgttcaaatg attcggtttt caggaacatc gctgactctc
      781 cggattaact ggcggtgacg ttgatttctg caaccgcagc aaactcacca ttaccagata
      841 cgaccggaat gttgaccttg cctgcagcaa cacctttcac ggtgatggtc ataccactga
      901 ccgacacggt ggcttttgtt ttatccgcag acaccgcacg gaagctcttg tcggttgcgc
      961 catccggctg gaatgccacg gtcagcgtgg tgctctgccc tttcactacg gaagcactgg
     1021 cgggtgtcac cgtcatgccg gtcgtcgccg tcaccgtgct gcgatcttct gccatcgacg
     1081 gacgtcccac attggtgacc ttcaccgtgc gggtaatcac ctccttcgcc gtcaccgcct
     1141 taccgatact gctgacccag cc
//
LOCUS       DAZPEP010000361         1157 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZPEP010000361 DAZPEP010000000
VERSION     DAZPEP010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.363"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            6..407
                     /locus_tag="ACHUT9_004575"
                     /pseudo
     CDS             6..407
                     /locus_tag="ACHUT9_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="ACHUT9_004576"
     CDS             572..>1157
                     /locus_tag="ACHUT9_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFR1350848.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          322 a          260 c          276 g          299 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc caggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAZPEP010000362         1155 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZPEP010000362 DAZPEP010000000
VERSION     DAZPEP010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1155
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.364"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>1155)
                     /locus_tag="ACHUT9_004577"
     CDS             complement(<1..>1155)
                     /locus_tag="ACHUT9_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1350849.1"
                     /translation="ASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELSTWDTIYLHL
                     ILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDNEVEIKAGN
                     ERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHTIGSKIANV
                     IFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIALMNTFYDS
                     DNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTRFSAFQDTV
                     FNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMVREDASNLV
                     NLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENGS"
BASE COUNT          367 a          219 c          175 g          394 t
ORIGIN      
        1 actgccgttt tcaaaacaac attttttcaa tatgctttca ttcacaacag tgtgtgataa
       61 gtagagatta cttatgtttt gctttattaa tgccacgtca ttaaaatgac tatatttaat
      121 gaaaaaacca tcattgagta gtgttttaag attgactaaa ttacttgcat cttcacgtac
      181 catcgtggca actggaacag gcagagacat gaaaacttct tcattcacca tcaagtgaat
      241 catgcattgc gttccctttg tcatatccac acatacgcta caaatatcgt ggtaatgcga
      301 agagcgcatt tcaggaaaca gattaaacac ggtatcttga aaggcactaa atcgtgttaa
      361 taaatcctga taatgaacag gttttgtttc aaaattatca tcagcaaatc caagcactat
      421 tgtatgctct acatctaacg cagtttcaaa tggctcttct tctgtaaaat cattatttaa
      481 aacctcacct gacgtattat cactgtcata aaaagtgttc atcaatgcga tattatttgt
      541 tacagcgtct ttttcgatat tctcactaat tgcatatcgc ctgctataat cataaaaatg
      601 aataaacgcg ccctttataa aggatatata ttgttctttg ttaaagtcaa tacggctgta
      661 taatttttca aaaatcacat tcgcaatttt actaccaatg gtatgcctgt ataataatgg
      721 gtgcgattca atcgtaaaat gcttgtctaa ttgatcttgt aatttattct catagtccag
      781 agggaaaaga aactgttgtc tttcaagttt cccattcgat acattacgta ttccaataga
      841 gattctttca ttacccgcct ttatttcaac ttcgttatct tctgatttac ccagatattt
      901 tttaacatca gtctcgaaag cattactaaa aaaagtatta aattggtaat tcgctgatgg
      961 catattttcc gcgagcaaca catcattttt tatgagatca ctgtcagcat ttattgctaa
     1021 tatcagatgc aagtagatgg tatcccatgt agacaactca cccttacgac tggaatttaa
     1081 tatcgcgaaa aattccctca gtgaatccag gaaattatca ctttgattta gttgtgtgcc
     1141 tggaaatgat gatgc
//
LOCUS       DAZPEP010000363         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZPEP010000363 DAZPEP010000000
VERSION     DAZPEP010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.365"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..122
                     /locus_tag="ACHUT9_004578"
     CDS             <1..122
                     /locus_tag="ACHUT9_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350850.1"
                     /translation="HFLPVDQESARVLSTPMKPGNPRRYSNKRTDSSKMERFF"
     gene            119..376
                     /locus_tag="ACHUT9_004579"
     CDS             119..376
                     /locus_tag="ACHUT9_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350851.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            376..750
                     /locus_tag="ACHUT9_004580"
     CDS             376..750
                     /locus_tag="ACHUT9_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFR1350852.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            747..>1148
                     /locus_tag="ACHUT9_004581"
     CDS             747..>1148
                     /locus_tag="ACHUT9_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1350853.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNK"
BASE COUNT          325 a          251 c          317 g          255 t
ORIGIN      
        1 gacattttct gcctgtggac caggaaagtg cacgggtgct ttccactccc atgaagcccg
       61 gcaatccgcg cagatacagt aataaacgaa ctgacagcag taagatggag cgtttctttt
      121 gaacaaccag acaatgactt ttacccctga acaattgcgt aaacacgcgc aggaaatgct
      181 gcgtcatgct gaacaactcg aaaaaacggg cataacaaaa gacgctatcc gtaaagatat
      241 ggtacccgcg cttcgggaac tgatgcaggc gaagcatcgc gcacagaaag cagtagatga
      301 gctggtggac tgtgtggcag agctggaaac cagagtcgga aagtttgaaa aactggtgca
      361 ggaggtgctg cgctgatgcg ccatgagttt attttacctt atccgccgac ggtgaatact
      421 tactggcgac gtcgtgacaa cacatatttt gtatcaaaag ccggtgagcg ttatcgccgg
      481 gatgtggcgc ttattgtccg tcagcagcga ctgaaattaa gcctgtccgg aagactggcg
      541 ataaagatta ttgcagagcc accggataag cgccgtcgtg acctggacaa tatcctgaaa
      601 gcaccactgg atgcactgac acatgcggga gtgttaatgg acgatgagca gtttgatgaa
      661 atcaatatcg ttcgtggtca gccagtatct ggtggacgtc tgggggtgaa gatttacccc
      721 ataatgcatg aagagcaggt caaaaaatga aactggaaga tttaccgaaa tactactccc
      781 caaaatcccc ttgcctgacc gatgcatcgg cctcaacgtc aaaagatgcg ctgagtatca
      841 ctgatgtgat ggccgcgcag ggcatgacac agaatcgggc tgagatgggg ttttctgcgt
      901 tcctggggaa aatgggcatc agtatgaatg acagggcgcg ggcaacagaa ttactggcag
      961 attatgcact cagtcggtgc gatcgtgtgg cggcgttgag aaaacttccg gcagaaataa
     1021 aaccggtagt gatgcgcatt atggcttcgt acgcttttga ggattatgcc cgcagcgcag
     1081 cgagtaaaaa gcagtgccct tgttgctatg gggaaaaatt tattgaaagc gtagttttta
     1141 caaacaag
//
LOCUS       DAZPEP010000364         1148 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZPEP010000364 DAZPEP010000000
VERSION     DAZPEP010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.366"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..187
                     /locus_tag="ACHUT9_004582"
     CDS             <1..187
                     /locus_tag="ACHUT9_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFR1350854.1"
                     /translation="REYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNN
                     YRIALRKYQHYQRQTCT"
     gene            320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHUT9_004583"
     CDS             320..1120
                     /gene="tcyJ"
                     /locus_tag="ACHUT9_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFR1350855.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          306 g          276 t
ORIGIN      
        1 gcgtgaatat gtcgttcgtc tgcgccgttt gggtaatcat ctacacgagc aaaatatttc
       61 cctcgatcag ctgcaggacg gtttccttga tgaaatcctc gccccgtggc tgcccaccac
      121 cagcaccaac aattaccgca ttgcgttacg gaagtatcaa cactatcagc gtcaaacgtg
      181 tacctgactt gtgcagaaat cctccagtct gcctgcttct gatatatatt aaataagaat
      241 aagatgtagc ggagttgttt ttgtgtttac aaacaatggc tctacactgc aaacagacat
      301 aacaacattc ggggtgaata tgaaattagc acatctggga cgtcaggcat tgatgggtgt
      361 gatggccgtg gcgctggttg cgggtatgag cgttaaaagt tttgcagatg aaggtctgct
      421 taataaagtc aaagagcgcg gcacgctgct ggtagggctg gaaggaactt atccgccgtt
      481 cagttttcag ggggatgacg gcaaattaac cggttttgaa gtggaatttg cccaacagct
      541 ggcaaaacat cttggcgttg aggcgtcact aaaaccgacc aaatgggacg gtatgctggc
      601 gtcgctggac tctaaacgca ttgatgtggt gattaaccag gtcaccattt ctgacgagcg
      661 caagaaaaaa tacgattttt ctacccctta caccatttcc ggtattcagg cgctggtgaa
      721 aaaaggtaac gaaggcacca ttaaaacagc cgatgatctg aaaggcaaaa aagtgggtgt
      781 cggtctgggc accaactatg aagagtggct gcggcagaat gttcagggtg tcgatgtgcg
      841 tacctatgat gatgacccga ccaaatatca ggatctgcgc gtaggccgta tcgatgcgat
      901 cctcgttgac cgtctggcgg cgctggatct ggtgaagaaa accaacgata cgctggcagt
      961 aaccggtgaa gcattttccc gtcaggagtc tggcgtggcg ctgcgtaaag gaaatgaaga
     1021 tctgctgaaa gcagtgaatg atgcaattgc ggaaatgcaa aaagatggca ctctgcaagc
     1081 cctttccgaa aaatggtttg gtgctgatgt gaccaaataa tcagcataat gacaaaaaag
     1141 ggcgcttt
//
LOCUS       DAZPEP010000365         1140 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZPEP010000365 DAZPEP010000000
VERSION     DAZPEP010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.367"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>1140
                     /locus_tag="ACHUT9_004584"
     CDS             <1..>1140
                     /locus_tag="ACHUT9_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFR1350856.1"
                     /translation="EREIDHLQAQLDKLRRMNFGSRSEKVSRRIAQMEADLNRLQKES
                     DTLTGRVYDPAVQRPLRQTRTRKPFPESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTA
                     EQLELMRSAFRVIRTVREKHACTQCDAIVQAPAPSRPIERGIAGPGLLARVLTSKYAE
                     HTPLYRQSEIYGRQGVELSRSLLSGWVDACCRLLSPMEEALHGYVLTDGKLHVDDTPV
                     PVLLPGNKKTKTGRLWAYVRDDRNAGSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVRQ
                     ADAYAGFNELYRDGRITEAACWAHARRKIHDVHVRTPSALTEEALKRIGELYAIEAEI
                     RGMTAEQRLAERQLKTKPLLKSLESWLREKMKTLSRHSELAKAFA"
BASE COUNT          250 a          323 c          362 g          205 t
ORIGIN      
        1 ggagcgcgaa atagaccatc tgcaggcgca gctggataaa ctccgccgga tgaacttcgg
       61 cagtcgttcc gaaaaagtct cccgccgtat cgcacaaatg gaagccgatc tgaaccggct
      121 tcagaaagag agcgatacgc tgactggtag ggtgtatgac ccggcagtac agcgtccgtt
      181 gcgtcagacc cgcacccgta agccgttccc tgaatcacta ccccgtgacg aaaagcgact
      241 gttgcctgcg gcgccgtgct gcccgaactg cggcggttca ctgagctatc tgggcgagga
      301 taccgccgaa cagctggagt tgatgcgtag cgccttccgg gttatccgga cggtacggga
      361 aaaacatgcc tgtactcagt gcgatgccat cgtgcaggca cctgcacctt cgcggcccat
      421 cgagcggggt atcgccggac cggggctgct ggcccgcgtg ctgacctcga agtatgcaga
      481 gcacaccccg ctgtatcgcc agtcagaaat atacggccgg caaggtgtgg agctgagccg
      541 ttcactgctg tcgggctggg tggatgcatg ctgccggctg ctgtctccga tggaagaggc
      601 gcttcatggc tatgtgctga ctgacggtaa gctccatgtt gatgacacgc ctgtcccggt
      661 gctgttgcca ggcaataaga aaacgaagac cgggcggttg tgggcgtatg ttcgtgacga
      721 ccgtaacgcc gggtcaacgc tggcgccggc ggtgtggttc gcttacagcc cggacagaaa
      781 aggcatccat ccgcagaccc atcttgcggg gttcagtggt gtacggcagg cggatgcata
      841 cgccgggttc aacgagctgt accgggatgg ccggataacg gaagccgcct gttgggctca
      901 cgcccgccgt aaaatccacg atgtgcacgt tcgcaccccg tcagccctga cggaggaagc
      961 gctgaaacgg atcggcgaac tgtacgccat cgaggcagag ataaggggaa tgacggcgga
     1021 acagcgcctt gccgaacgtc agttgaaaac gaaaccgctg ctgaaatccc tggaaagctg
     1081 gctgcgtgaa aagatgaaaa ccctgtcgcg acactcagaa ctggcgaaag cgttcgcata
//
LOCUS       DAZPEP010000366         1123 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZPEP010000366 DAZPEP010000000
VERSION     DAZPEP010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.368"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..988)
                     /locus_tag="ACHUT9_004585"
     CDS             complement(<1..988)
                     /locus_tag="ACHUT9_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1350857.1"
                     /translation="MNDRLCFEVHDNKGYFVFSDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWP"
BASE COUNT          297 a          262 c          248 g          316 t
ORIGIN      
        1 agggccagat atattcaccg ttcatatttc tgcatttctg tacaatttcc tcagaaagtg
       61 tcttgtcaat tcttttccac aggtgctcct gctgtcttaa aatactttcg catatttcga
      121 aatcatcttc ctgaatcagc atttcggcgc atttaatgat ttctgatcgt tcatgcagca
      181 cggaggaatg gttatagagc cagtttacaa acagcaatgc ttccggatat tgtccccata
      241 atggtgagta tgttgcatag taatcttctg ccgtatccag agagccggag aagtcatgcc
      301 acacagcaag tggaaatgga tgcagatttc cgcacagcat ttctgcgatg taagtgtttg
      361 tggcaaagcc atggcgaaaa gctgtggcgg ccttaacatg ttccccgttc agaaaatgca
      421 ggagaccgag ttcataccag taaggtgaaa attcatcagc atgctctttc agtacactga
      481 aggctcgctc atgatcgccg gtgcgtaaca gctctgaacc caacaaccag cgagccccct
      541 gattatcttc aggattgtat gccagtattt tatcagtgag cataaccgca tcctgatggc
      601 gctgtaagtg cacatttgcg agaattgctg cataaagcgc gcgcagataa ggccggtttt
      661 cgggatgcat ccagattatt tcaccacaga aactttccgg gatgatacga ttacctgccg
      721 ccagtccttt aagagaggcg ttcagtgcct tacgcggggc attctgttcc agaaatgcat
      781 atgccagatg agcgtgtata tcaataaagt ccggctcccg ttgagcaagg cgtcggagtt
      841 tgttgatgta actcgtctcg ctgatttcat cagcgtcgta tgcatcaagc acttcttcaa
      901 actcccccag caatgggcca aaccaggtat ccgaaaatac gaaataaccc ttgttatcgt
      961 gcacttcaaa gcataatcta tcgttcattt ctgagcctgt aaaacatgtg gtgatatagt
     1021 ctgagagcat aaaagttgag attctgtgta tcaagtcctg tcttggcacc gaatgaatat
     1081 tgcttacgaa gccccttcat gctttccttg agaacaataa ttt
//
LOCUS       DAZPEP010000367         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZPEP010000367 DAZPEP010000000
VERSION     DAZPEP010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.369"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..906)
                     /locus_tag="ACHUT9_004586"
     CDS             complement(<1..906)
                     /locus_tag="ACHUT9_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HFR1350858.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          325 a          245 c          216 g          331 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagtgcg tcataattca agttaataac cttaagggat accagttata
     1081 tttaaactaa attatagtaa tgaataattt tcttata
//
LOCUS       DAZPEP010000368         1117 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZPEP010000368 DAZPEP010000000
VERSION     DAZPEP010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.370"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            84..1115
                     /locus_tag="ACHUT9_004587"
     CDS             84..1115
                     /locus_tag="ACHUT9_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HFR1350859.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAZPEP010000369         1113 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZPEP010000369 DAZPEP010000000
VERSION     DAZPEP010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1113
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.371"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..452
                     /locus_tag="ACHUT9_004588"
                     /pseudo
     CDS             1..452
                     /locus_tag="ACHUT9_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            567..>1113
                     /locus_tag="ACHUT9_004589"
     CDS             567..>1113
                     /locus_tag="ACHUT9_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1350860.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAH"
BASE COUNT          420 a          214 c          160 g          319 t
ORIGIN      
        1 taaaaaataa tgccagttat caacttaata acgcattacc taacaaagaa accatctcca
       61 gcaatttttg tgaacgacta gaacaatggg gtaataaatc ggttaataat ggtgaagaaa
      121 gagcaattgc cgtggagcga attaaagaag cttacaattc gaacatggca tctcttgatt
      181 tgtcatatct tgatttgagt gaattaccgc ctatcccctc gacagttaat acgttaaatc
      241 tggaaaacaa ttgtctaact tgtcttgact tagctgacaa tgccagtctc gttaatatca
      301 acctcagcgt taataaaatt aacacgataa cctttccaaa agaatcaaaa ctggaaaaca
      361 tttatattga tcataacaat ctggaaagtt tagatttaaa aaatcagcat tcattggtta
      421 atctggaagc gcaaaataac aaactgacaa aaattaatat ttctgatagt tataaactga
      481 aatttcttaa tcttgattat aacaagctag catcgctgga tgtctcccgg caagaatctc
      541 tgattgagtt aagtgcccat cacaatatga tcactgacct tatattacac aatcatccca
      601 ggatgaaaac aatcacttta aacgacaacc atattgcaca tttaaacgcc aaaaacacta
      661 caaaactgga atatttaaac ttaagcaata acaatttact gccaaccaat gacattgatc
      721 aactaatatc atcaaaacat ctttggcatg tattagttaa cggcatcaac aatgatccac
      781 ttgcacaaat gcagtactgg actgcagtaa gaaatataat tgatgacact aatgaagtga
      841 ccattgattt atcaggactt aatttaacca ctcaaccacc agggctgcaa aacttcacat
      901 ctatcaatct tgataataac caactcacac attttgatgc aaccaactac gatagactcg
      961 taaagctaag tctgaatagt aatgctcttg agtcaataaa ttttcctcaa ggcagaaatg
     1021 taagtattac acatatatct atgaataata atgctctcag aaatattgat atagataggc
     1081 tttcatcagt tacttatttt agtgcggcac ata
//
LOCUS       DAZPEP010000370         1088 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZPEP010000370 DAZPEP010000000
VERSION     DAZPEP010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.372"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            529..>1088
                     /gene="fimB"
                     /locus_tag="ACHUT9_004590"
     CDS             529..>1088
                     /gene="fimB"
                     /locus_tag="ACHUT9_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFR1350861.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTTSNAGRFYG"
BASE COUNT          333 a          189 c          233 g          333 t
ORIGIN      
        1 tccaggataa aaaggaatgt aacaatctca tggcgtaagc tgacgaacca ggcaggaata
       61 atcgctaggg acctaagcat tagcatgata atagccacta agaaattact gtgttccatg
      121 aaatagccat tttgtggcaa atggagttga ctaataatgt catatgtgag acggctagtt
      181 gaacgaatat taaattttgc tgaatttttt atgttgattt tacttgttac agaacatatc
      241 acatgatata tagataagat tagttgcatt aatgatgagg gttattatta gattcgtatc
      301 aggttgataa atatataaag gtacatagca tgcaagagca tggcgtttgt atggcaaagt
      361 tattataagt aactatcgct actccattta agttcgctca gaagaactgg tccacttacg
      421 ttagttatta agcaaacgtt cgcttttata aacataatca ggattaaaat gttggattat
      481 tgctaaccca gcacagctag tgcgcgtctg taattataag ggaaaacgat gaagaataag
      541 gctgataaca aaaaaaggaa cttccttacc catagtgaaa tcgagtcact ccttaaagca
      601 gcaaataccg ggcctcatgc agcacgtaat tattgtctga ctttgctttg ttttattcat
      661 ggtttccggg cgagtgaaat ttgtcgattg aggatttcgg atattgatct taaggcaaag
      721 tgtatatata tccatcgatt aaaaaaaggc ttttcaacaa cgcacccgct attgaacaaa
      781 gaagttcagg ctttaaaaaa ctggttgagt atccgtactt cttatccgca tgctgagagc
      841 gagtgggtat ttttatcacg taaggggaat ccgctttctc ggcaacagtt ttaccatatt
      901 atctcgactt ccggtggtaa tgccgggttg tcactggaga ttcatccgca catgttacgc
      961 cattcgtgtg gttttgcttt ggcgaatatg ggaatagata cgcgacttat ccaggattat
     1021 cttgggcatc gcaatattcg tcatactgtc tggtatacca ccagcaatgc agggcgtttt
     1081 tacggcat
//
LOCUS       DAZPEP010000371         1087 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZPEP010000371 DAZPEP010000000
VERSION     DAZPEP010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.373"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            35..190
                     /locus_tag="ACHUT9_004591"
                     /pseudo
     CDS             35..190
                     /locus_tag="ACHUT9_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
     gene            294..>1087
                     /locus_tag="ACHUT9_004592"
     CDS             294..>1087
                     /locus_tag="ACHUT9_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFR1350862.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSL"
BASE COUNT          315 a          223 c          254 g          295 t
ORIGIN      
        1 ataacagatg tctggaaata taggggcaaa tccaggtact gccgaaaata ttcagataga
       61 gctgagggat gaccaggatg ctgcgttaaa aaatggcgat agcaaaacgg ttattgttga
      121 tgagatcact cgtaatgcac agtttccact taaggcaaga gctatcacgg tgaatggaaa
      181 cgcaagctag ggaacgatcg aggcgctaat caatgtgatc tacacctggc aataacgtcg
      241 attcgatttt cttagccttc tttttaaatc aatttttaac cttaggatat atcatgggta
      301 aaacaatcag tataaaggtc ctgttcggta tttatcttct attaatggca ggcaaagttt
      361 ttgccttcag ttgtaatgtc gatggcgggt caagtattgg agcaggtaca acctctgttt
      421 atgttaatct cgaccctgta atacagccgg gccagaatct ggttgtagac ttgtctcagc
      481 atataagttg ctggaatgat tacggcggct ggtacgacac tgatcatata aacctggtac
      541 aaggctcagc tttcgcaggc tccctacagt catataaagg atcgctgtac tggaataatg
      601 tgacctaccc cttcccgttg acgacaaata ccaacgtatt ggatattggt gataagacac
      661 cgatgccatt accgttaaaa ctctatatta ccccagttgg ggcggccggt ggagttgtca
      721 taaaagcggg ggaagtcatt gcccgtattc atatgtataa aattgcaaca ttaggaagcg
      781 gaaatccgcg taattttacg tggaatatta tttcaaataa cagtgttgtt atgccaacag
      841 gcggctgcac cgttgatagt cgtaatgtca ctgtggatct cccggacttt cctggaagcg
      901 cagaaatacc ccttggcgta tattgctcaa gcgaacaaaa gctctcattt tatctttcgg
      961 gtgccaccac tgacagtagc cgacaagtgt ttgctaacac agcacctgat gcaacaaaag
     1021 catcgggagt gggtgtcact ctaatgcgta atggaaaaat actggctacg ggagaaaatg
     1081 tctccct
//
LOCUS       DAZPEP010000372         1086 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZPEP010000372 DAZPEP010000000
VERSION     DAZPEP010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.374"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(506..>1086)
                     /locus_tag="ACHUT9_004593"
     CDS             complement(506..>1086)
                     /locus_tag="ACHUT9_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1350863.1"
                     /translation="RLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCLPQNSELIY
                     CWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRCYNLMLSEP
                     GTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIA
                     RENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          343 a          207 c          190 g          346 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacggt
//
LOCUS       DAZPEP010000373         1085 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZPEP010000373 DAZPEP010000000
VERSION     DAZPEP010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.375"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..643)
                     /locus_tag="ACHUT9_004594"
     CDS             complement(<1..643)
                     /locus_tag="ACHUT9_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1350864.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHUT9_004595"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="ACHUT9_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFR1350865.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAZPEP010000374         1073 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZPEP010000374 DAZPEP010000000
VERSION     DAZPEP010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.376"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..1073
                     /locus_tag="ACHUT9_004596"
                     /pseudo
     CDS             1..1073
                     /locus_tag="ACHUT9_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          416 a          212 c          157 g          288 t
ORIGIN      
        1 cggcgatatt tataactgca caacaaatac cgataatgct aaaacgccag acacaccgaa
       61 gtggccttgc gataattggg aagaacaaca acccattaat tccacattct ctggagaagg
      121 atatatctct gatcaatatg atttagtgca acaccaactt caacaaatta atgcgtgcca
      181 cacgaacact acatatacaa atgcagacta ctcaaaagtt gtggctcaac ttgtaagtct
      241 tattactaac attgaaacga taagttcgac acaacttacg caacagacac aatcaatact
      301 taatcagatt aataacatta gatatgaaaa aaacaaaagt gctgaatgtc gaatcattgt
      361 tgtcgctaat cctaaacccg acaaggcaat cataactaaa attagcgtag aagagggaat
      421 acccataaca ttcagtgtcc aaaccatgtt ttcagatact aattttattg ctgaacaacg
      481 agctgactta ccaactaata taaaagatat tcaaagcctg tatcaaaaaa tgacgaagtt
      541 atatattgag cacagtgaaa ataaaaacag gatgaaagtc tttgccggag ccaactttat
      601 tgatttcaat atgacaggac agaacctgtc cgggttcgtt ctaaccttgt caaggtttta
      661 tttcgaagat ctacttaata taaatttcac tgacgcaaat ttaggtgaca ctatttttta
      721 cataaagaac accctacccc caaattatat aaagatggac aatatcttga caaacaaatc
      781 gaaggtttat tttcaacatt attaaccatt aatgacaact ttttacgagc aaaagcggaa
      841 attgcttcga caataattaa atttttagag gccaggataa ccaatctttc atacaatgac
      901 atattaaaat atcagcaaga attccaaaaa caatgttata agcaagttaa agcctttacg
      961 acactctctc gatacaataa aattcaaaca tgggcagaaa tgtctgaata tcagttcgaa
     1021 gtatttcaat acgagacgct taaccccaaa aaaatgtcac acacgcctta ttt
//
LOCUS       DAZPEP010000375         1070 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZPEP010000375 DAZPEP010000000
VERSION     DAZPEP010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.377"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(145..>1070)
                     /locus_tag="ACHUT9_004597"
     CDS             complement(145..>1070)
                     /locus_tag="ACHUT9_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFR1350866.1"
                     /translation="DTIARVVSCISPAKFHECFINWMRDCHSTDDKDVIAIDGKTLRH
                     SYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDA
                     MGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSEKSHG
                     REEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYISSAD
                     LTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINVLTND
                     KVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT          283 a          246 c          192 g          349 t
ORIGIN      
        1 tccattttgt tctccttaat tatgtaagga gtatttgatc atgtataagc aataaaaaac
       61 agcttcaggt aataaggaat atctcaattt ttaaacataa aatgcgaatt atttagtaca
      121 aaaagtgaac tcctcagggc aagattacga aagtccgctc cccgcaagga ctgacgcgag
      181 atagtttctg tccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt
      241 atcattcgtc aaaacattaa tagcaatgtg ccgtatccct gaaaataatt ctgctgcatt
      301 tcctcttctt attttgcagt cgtcttcatt cattaccacg tccagacgcc agtgcagatt
      361 attctccacg tgccagtgat ttcgaatcgc tgtcgcaaac ttctcagcgg ttaaatcagc
      421 agaactgata taatatctga ccatcatttc gggctctttc ttttgctctg ctattattga
      481 gcgaaaggag actgccacgc ataatttttt cagccctttc cattcaaacg tgaaatcaat
      541 aagttcatca gggacatcgc aaacaatatg aagacggatt tcttctctgc cgtgactctt
      601 ttcactcatt gcggaactgt catgcgctgg attatttaat tctgtcagcg gaaatttttt
      661 ctcaaaggct ttatttagcc gcccctggtt tccttttacc gcgaataaat aatcacctcc
      721 ctgtttttgt atcttctctg caatatcttt ctggcaaccc atcgcatcag ttgtgatgat
      781 ttttccttta atatccagca tgttaagaag ttctgggata gctgtaatct cattagattt
      841 ctcatccgtc ttgatctgtc cgatgaccag actgtgcatt gttgagaacg cactaatgac
      901 atgaatcgct cccctgcggc gactcttgtc ataagagtgc cggagcgttt ttccatcaat
      961 tgcaatgacg tctttatcat ctgtagaatg gcagtcacgc atccagttaa taaagcactc
     1021 gtgaaatttt gcaggactga tacaggatac aactctggca atggtatcgt
//
LOCUS       DAZPEP010000376         1045 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZPEP010000376 DAZPEP010000000
VERSION     DAZPEP010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.378"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            108..257
                     /locus_tag="ACHUT9_004598"
                     /pseudo
     CDS             108..257
                     /locus_tag="ACHUT9_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            284..388
                     /locus_tag="ACHUT9_004599"
                     /pseudo
     CDS             284..388
                     /locus_tag="ACHUT9_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            728..985
                     /locus_tag="ACHUT9_004600"
     CDS             728..985
                     /locus_tag="ACHUT9_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071468 - cellular response to acidic pH
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development regulator YmgB/AriR family
                     protein"
                     /protein_id="HFR1350867.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT          357 a          180 c          198 g          310 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tatcgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct gcttcctgtt cttacaataa tcagtattat gtattttatt tatatagcaa
      661 aatatgcttg ttacacctgt cataattagg catggtaaaa ttattgactg aaccctgggg
      721 gaggaagatg cacagtgatt attataatat ggtttttggt gaaaaattgg ccaatatttt
      781 atatgaggct aactctcaat ttttttacga gagaaatgtc atagaagagg cagtcaacgc
      841 tttattctgt gagcgtgaaa taataaacaa caagaacata ataaagaagc tgatgttctt
      901 tttgtcagat gttaatcaca ccaagaaaga tgttgttcag agcgcactta atattatcat
      961 tgacattaca tcaggtgata tttgagattt tacataaaag ataatctctc aaggagtctt
     1021 atctgaaaac gaaatatata gtccc
//
LOCUS       DAZPEP010000377         1042 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZPEP010000377 DAZPEP010000000
VERSION     DAZPEP010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.379"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            81..296
                     /gene="xisR"
                     /locus_tag="ACHUT9_004601"
     CDS             81..296
                     /gene="xisR"
                     /locus_tag="ACHUT9_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFR1350868.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            298..>1042
                     /locus_tag="ACHUT9_004602"
     CDS             298..>1042
                     /locus_tag="ACHUT9_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFR1350869.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKG"
BASE COUNT          352 a          192 c          243 g          255 t
ORIGIN      
        1 aaaacttcat agctgattat taataatcaa catcgggcgt caatttcagc ctaacattgg
       61 cgcctgccag aggtgatgcg atggcacaag taatctttaa tgaagagtgg atggttgaat
      121 acggcctgat gcttcgcact ggtctgggtg ccagacaaat tgaagcatac cgccagaact
      181 gttgggtgga aggcttccac ttcaaacgag tatctccttt agggaagcca gacagtaagc
      241 gagggattat ctggtacaac tatccgaaga taaatcagtt tatcaaagac tcatgatatg
      301 tctaaattac caacaggtgt cgagattcga ggtaaataca ttcgcatctg gttcatgttt
      361 cgaggaaaac gatgtcggga aacattgaaa ggctgggaga ttactaacag taacattaaa
      421 aaggccggga atttaagagc gttgatagtt catgaaatca gttccggtga atttgagtat
      481 ttaagacgtt ttccccagtc cagcactggg gcaaaaatgg tgacaacgag ggtcataaaa
      541 acattcgggg agctttgtga tatctggaca aaaattaaag agacagagtt aacaacaaac
      601 acaatgaaga aaacgaaatc acaattaaaa acactcagga taataatttg tgagagtacc
      661 ccgatatcgc atattcgtta tagcgatatc ttaaactacc ggaatgaact gctgcatgga
      721 gaaacgcttt acctagataa tccaagatcc aacaaaaaag gaagaaccgt gcgcacagtt
      781 gataactata tcgccctgct ctgttcgttg ttacgttttg cgtatcagtc gggatttata
      841 tcaaccaaac catttgaagg agtaaaaaaa ttacagcgaa acagaataaa gcctgacccg
      901 ttatctaaaa cagaattcaa tgcattaatg gaaagtgaaa aaggacagag ccaaaacttg
      961 tggaaatttg ccgtttactc cgggcttcgt cacggggaac tggcagctct ggcgtgggag
     1021 gatgtggatt tcgagaaggg aa
//
LOCUS       DAZPEP010000378         1035 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZPEP010000378 DAZPEP010000000
VERSION     DAZPEP010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.380"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..617
                     /locus_tag="ACHUT9_004603"
                     /pseudo
     CDS             1..617
                     /locus_tag="ACHUT9_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
     gene            614..>1035
                     /locus_tag="ACHUT9_004604"
     CDS             614..>1035
                     /locus_tag="ACHUT9_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFR1350870.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTE"
BASE COUNT          368 a          177 c          157 g          333 t
ORIGIN      
        1 tccggaacaa aaatatcaca acgtgaatgc actgatattt tatttgcaga aatgaaagaa
       61 ctagcaaaaa tgttttcctt ttttggacaa tataaaacac ttatcgaaga tctgattgaa
      121 cattttagat atggaaatgg cagcaatttt catagccaac aattaaattt atcttttcat
      181 gaaaaaataa atatggttat aatagtccaa tacgaataat caaagaatgt atcgaaaatg
      241 gcattaattc aactccctcg acaggatacc aacctcttat cctgcaaagc attaaaacca
      301 aattactttc atcaagacta aataaattta atgacttcga ggacagtttt aatggcttag
      361 gaatcagtgt tcatgatatt tcagctcaaa aaatatctct tttaagtttt caaaaatacg
      421 ctataggatg gagtgcaaca attcattttg tagcacagga ccacttcggt cttgatgtaa
      481 ctgatatcaa aaacaagact tacagcaaat atagattttt tcgcatatgg ttctttttac
      541 aacggcataa agactttgct ttcaaacctt ttttcaccaa ttttaacact atcgaaagaa
      601 tcgaaaatta tctatgaaaa gcaaactcca gaaaattata ttatgtttat ttcttctatg
      661 ctgcatctat aatttatgga cattgcgtcc tgtacagatt ttgtatacat actctgatgc
      721 tggcaattca gtctttctgg tcgttgatca tctgccatgg actgacagcg ataaaataaa
      781 ttggtatctg aagcatcaga atgaaattaa aaatcaacat cccctaccag aaggtagttg
      841 gcatacctgg tatgtcattg atatcggtaa tggcttcact gactataaaa aatatatcga
      901 agggccgtat gaagatctct actgtttccc gacaattaag agcaatgata attgcatcgt
      961 gaaaaattac ttaatggtta ttaatgaata cccttatcgt aacactcata ttggtattaa
     1021 cgattttact gagta
//
LOCUS       DAZPEP010000379         1033 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZPEP010000379 DAZPEP010000000
VERSION     DAZPEP010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.381"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..120
                     /locus_tag="ACHUT9_004605"
     CDS             <1..120
                     /locus_tag="ACHUT9_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1350871.1"
                     /translation="EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            120..410
                     /locus_tag="ACHUT9_004606"
     CDS             120..410
                     /locus_tag="ACHUT9_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFR1350872.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            407..961
                     /locus_tag="ACHUT9_004607"
     CDS             407..961
                     /locus_tag="ACHUT9_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFR1350873.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          294 a          197 c          294 g          248 t
ORIGIN      
        1 gagtttcagc aggtttataa atctgttctg aatgtgctgt ggaactggat tctgttccgt
       61 aaattttcct ctccggaaca agtcgaaaat gtggccgcgc agctgctgga gtttgcgtaa
      121 tggtggattt acgtaaagcg gcgcgggggc agatgtgcac cgtcagaatt cctggctact
      181 gcaatcacga tccggaaacg tctgtgctgg cgcattaccg actggcggga acgtgcggaa
      241 cagcgataaa gccacacgat atgcaggcag cgattgcctg tagctcgtgc cacgatttaa
      301 tcgacgggcg ggtaaaaacc agcgattaca ccaaagaaga attacgcctg atgcatgcag
      361 aaggtgtttt tcgcacacaa gaaatctgga gaaaggaggg atatttatga tttacccaac
      421 gaatacagga aaaagcggag aacaccttcg tctcaccacg ctggaaagtg tctggattca
      481 gggaaaacta cgtatgtggg ggcgctggtc gtatattggt ggcggtaaga cggggaatat
      541 gtttaacctg atgttgacct ctaaaaagct gacaaaaacg gcaattaacg aggcgctccg
      601 gaggatgaaa aaagcaggtc tgaacaagtc tgaacttgag gcttttttgc gggatatgat
      661 taacggtaag caaaagagct ggctggcgca ttgtactgat gcagaggcgt tatgtattga
      721 tcgggtcata agtgaggtgc tggcagagca tccaggattg attagcgtcc ttcggcaacg
      781 gtatgagggg cgggggatga ccaaacgcaa aatggctgaa ctgctgaatg atgcacaccc
      841 gaaatggagt ttaagaacct gtgaaagacg cattgagcat tggctaaagg tggcagaatt
      901 tattttgtac aaaccaatgg ttatggcttt tggtatagag aaaaaagtta ttgctttttg
      961 acgtaaaaac tgcttcaatt cttgtacgct tcgcaaagct gtaccgcgag gcgaatagca
     1021 gacatggaca ttt
//
LOCUS       DAZPEP010000380         1024 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZPEP010000380 DAZPEP010000000
VERSION     DAZPEP010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.382"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..228)
                     /locus_tag="ACHUT9_004608"
     CDS             complement(<1..228)
                     /locus_tag="ACHUT9_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFR1350874.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            409..561
                     /locus_tag="ACHUT9_004609"
                     /pseudo
     CDS             409..561
                     /locus_tag="ACHUT9_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
BASE COUNT          335 a          169 c          192 g          328 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgga
      961 tgatttgaac atttttacat tgttttatat atataattgt tttgaatata tattacctga
     1021 atta
//
LOCUS       DAZPEP010000381         1023 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZPEP010000381 DAZPEP010000000
VERSION     DAZPEP010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.383"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(77..865)
                     /locus_tag="ACHUT9_004610"
     CDS             complement(77..865)
                     /locus_tag="ACHUT9_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFR1350875.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="ACHUT9_004611"
     CDS             complement(905..>1023)
                     /locus_tag="ACHUT9_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFR1350876.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAZPEP010000382         1017 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZPEP010000382 DAZPEP010000000
VERSION     DAZPEP010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.384"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(1..441)
                     /locus_tag="ACHUT9_004612"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="ACHUT9_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197506.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
     gene            complement(458..>1017)
                     /locus_tag="ACHUT9_004613"
     CDS             complement(458..>1017)
                     /locus_tag="ACHUT9_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1350877.1"
                     /translation="RCKTRWTRCTRRREKIGLKGGWNFYQYPLNPITDIDPLGLATCL
                     YSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNNMQCKNNPGCTHLQNRGPIPQGV
                     WSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLTQSCLNAFGPSLGPRFCSEGCIT
                     GSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          294 a          203 c          171 g          349 t
ORIGIN      
        1 atatttttat catcactaca aatattaact gaatttatat caatgatttt tttattctta
       61 tcttttattt actgtttcaa tgcaatcagc ggcaagttgg agtggattta gatttttcag
      121 atatggtctt atgatctttc ccttacttaa taaatagcat gtataggatt cggaaagtcc
      181 agcatctatt ttgtacctca ttatattaga taaggatgtg agaaattcct tgttatcatt
      241 cgcagccata tatgccagtc cgagttcagc ctctgcggcc ttacaggcaa aatagcttag
      301 tgaacacgct tttcgtccct ctttagtact ggctgatatt agtgcccata atattgcttt
      361 gtctgccaaa tctaactctc tctgaaattg tcttgcagta actgtactcg cagaaataga
      421 gaaaaaaata caattgttaa taactttgcc atatttttta gtccgtaact gttaatgtgc
      481 tatctggctc tgaaaatatt aactcattta atttttgcat gtcattagat gaaccagtta
      541 tacaaccctc ggaacagaat cgaggtccta aagacggacc gaaagcgttt aaacaacttt
      601 gtgttaaaaa tccatcacga ttatatgttt ctgtatttgc tgacggtact aaacgtatac
      661 cattaggttt tctattggtt gctccaggac cattaacatt ccagctccat accccttgag
      721 gtattggacc tctattttgt aagtgtgtac aaccaggatt atttttacat tgcatattat
      781 taccattatt accagaggcg acaggaatag taagcacatc ataagagtta tcatcatttt
      841 gcgtatctga aacgcatgaa agcatactta aagttatgct gtataaacag gtcgctaatc
      901 ccaacgggtc aatatcagtt atcggattca acggatactg ataaaaattc catcccccct
      961 tcagtccaat cttttcgcgc cggcgtgtac accgggtcca tctggttttg catctgc
//
LOCUS       DAZPEP010000383         1009 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZPEP010000383 DAZPEP010000000
VERSION     DAZPEP010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.385"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..164
                     /locus_tag="ACHUT9_004614"
     CDS             <1..164
                     /locus_tag="ACHUT9_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFR1350878.1"
                     /translation="SLCIDHLQRHGANKQAISHAFDDDVEFQERMAEHIRYMAETIAH
                     HQVDIDLED"
     gene            170..>1009
                     /locus_tag="ACHUT9_004615"
     CDS             170..>1009
                     /locus_tag="ACHUT9_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFR1350879.1"
                     /translation="MALFQRAINTQAYLKAGIMGFAGDGKTYTASELAIGLVMLMRQR
                     GIEAGNRPVMFLDTETGSDWVKPRFDAENIELFTAKTRSFVDLLEAINEAESSGSVMI
                     IDSISHFWTGLCDEYARRRNRKRGLEFSDWAWLKQEWRRFTDRFVNSQAHIIMCGRAG
                     YEYDFFEGDDGKRQLAKTGIKMKAETETGYEPSILIQMEKQMNIETGQVWRTARILKD
                     RSTRIDGQVFSNPTFKNFLPHIEFLNLGGEHLGIDTSRDNSELFANDGTPTWLKEKRA
                     KEIA"
BASE COUNT          306 a          229 c          246 g          228 t
ORIGIN      
        1 aatcgctctg catcgaccat ttgcaacgcc acggtgccaa taaacaggca atcagccacg
       61 catttgatga tgatgttgag tttcaggagc gcatggcaga acacatccgg tacatggctg
      121 aaaccattgc ccaccaccag gttgatattg atttggagga ctaaaatcta tggcgctttt
      181 tcaacgagca ataaacacac aggcctatct aaaagcagga attatggggt ttgctggcga
      241 cggcaaaacc tacacagcaa gcgaactggc tattggtctg gtaatgctta tgcggcaacg
      301 gggaattgaa gctggtaacc gcccggtaat gtttctggac acagaaacag gctctgactg
      361 ggttaaaccc cgctttgatg cggaaaatat cgaattattt acggcaaaaa cccgctcgtt
      421 tgttgatctg cttgaagcca ttaatgaagc ggaaagcagc ggttccgtaa tgatcatcga
      481 ctccatcagt cacttctgga ccggattatg cgacgagtat gccagacgcc gaaaccgtaa
      541 acgaggactg gaattttcag actgggcgtg gctcaaacag gagtggcgac gctttaccga
      601 tcgatttgtt aacagtcagg cgcacattat tatgtgcggg cgtgcaggat atgagtatga
      661 ctttttcgaa ggtgatgatg gcaaacgcca gttggcaaaa accggtataa aaatgaaagc
      721 agaaacagaa accggttatg aaccctccat cctgatccag atggagaagc aaatgaacat
      781 agaaaccggt caggtgtggc gcaccgccag aatactaaaa gatcgctcca cccgtattga
      841 tggacaggta ttttcaaacc caacatttaa aaactttctt cctcatatcg agtttcttaa
      901 cctgggaggt gaacacctcg gcatcgatac ctcacgggat aacagtgaat tatttgccaa
      961 cgacggtaca ccgacatggc taaaagaaaa gcgtgcaaaa gaaatcgca
//
LOCUS       DAZPEP010000384         1002 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZPEP010000384 DAZPEP010000000
VERSION     DAZPEP010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.386"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..120
                     /gene="rcbA"
                     /locus_tag="ACHUT9_004616"
     CDS             <1..120
                     /gene="rcbA"
                     /locus_tag="ACHUT9_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break reduction protein RcbA"
                     /protein_id="HFR1350880.1"
                     /translation="SKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLLSS"
     gene            225..449
                     /locus_tag="ACHUT9_004617"
     CDS             225..449
                     /locus_tag="ACHUT9_004617"
                     /inference="COORDINATES: protein motif:HMM:NF019442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1350881.1"
                     /translation="MYLTLKEWNARQLRPRSPETVRRWVRECKIFPPPIKDGREYLFH
                     ESAKKITPQTTGGLLQRIRNGRTKKKLKHT"
     gene            340..>1002
                     /locus_tag="ACHUT9_004618"
     CDS             340..>1002
                     /locus_tag="ACHUT9_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFR1350882.1"
                     /translation="MNIYFMSQQKKLHLKRLAGFCRESEMAGRRRNSNTRDLPPNLYN
                     RGGYYSYRDPRSGREFGIGRDKRIAVNEAIAANMELLSTQHISLLDRIKGNSVTLFCE
                     LILNFRNEIERRQLSTNTMKRHNQRLKIISEYFGGVPVKNIGIREVYSFLEIRAAGSK
                     FAIANQYRALLSDIFKTAIASGLAEEDPASATRPFRTEVKRSRLLIDEYLLIRKIADV
                     QNE"
BASE COUNT          353 a          183 c          219 g          247 t
ORIGIN      
        1 tctaaactaa cgaaatcaga atgcgaaaaa atgctctcag ggaaaaaaga agcaggcgtt
       61 tccagagagc agaaagtaaa actgataaat tttaattgcg agaaacttct gtcctcgtga
      121 gttgcattat atcaaaatta gaacttcata gctgattatt aaaaatcaac cacgcccgcc
      181 agtattttgt atatttactg gcggtcatat tacaggaata tgacatgtat ttgacactca
      241 aagaatggaa cgcccgtcag ttacgtccac gaagcccaga aacggttcgc cgctgggtgc
      301 gagaatgtaa gatttttcca ccaccgataa aggatgggcg tgaatattta tttcatgagt
      361 cagcaaaaaa aattacacct caaacgactg gcgggctttt gcagagaatc agaaatggcc
      421 ggacgaagaa gaaactcaaa cacacgtgat ctaccaccca atctttacaa tcgaggtgga
      481 tattatagtt atcgtgaccc acgttcagga agggagtttg gaatcggaag agacaaaaga
      541 attgcagtta atgaggctat tgcggcaaat atggaattat tatccacgca acatataagc
      601 ttactggata gaataaaagg taatagcgta acattattct gcgagctgat attaaatttc
      661 agaaatgaaa tagagcgtag acaattaagc acaaacacca tgaaaagaca caaccagagg
      721 ttgaaaatca taagtgaata ctttggtggt gttccggtta aaaatatagg cataagggag
      781 gtgtacagct ttcttgaaat cagagcagct gggagtaaat ttgctattgc taaccagtac
      841 agggcgcttt tatcagacat ttttaaaaca gctatagctt ctggtcttgc cgaagaagat
      901 ccagcttctg caaccaggcc attcagaact gaagtgaagc gcagtcgact gttaattgac
      961 gagtatctgt taatcaggaa aatagcagac gtccagaacg aa
//
LOCUS       DAZPEP010000385          975 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZPEP010000385 DAZPEP010000000
VERSION     DAZPEP010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..975
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.387"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..975
                     /gene="espL4"
                     /locus_tag="ACHUT9_004619"
                     /pseudo
     CDS             1..975
                     /gene="espL4"
                     /locus_tag="ACHUT9_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
BASE COUNT          346 a          187 c          172 g          270 t
ORIGIN      
        1 ctcgtagttc tttctctgca aatattaata atacagccca gacaaatgaa caccaaaccc
       61 tgagtgaatt gttttataaa gaactcgagg ataaattttc tggcaaggag ctggcgacgc
      121 ctctattaaa aagcttctca gagaactgta gacaaaatgg tcgtcatatc tttagcaaca
      181 aggattttgt cattaaattt tccacgtccg tcttacaagc tgataagaaa gaaattacga
      241 taattaataa aacgaaaaca cgacactcac tcaaaccatt gctccaatat ttgaagaata
      301 cctaatggaa attttacctc aacgctcaga cactcttgat aaacaagaat taaacctaaa
      361 atcagataga aaagaaaaag aattcccaag aattaaactt aatggtcaat gttattttcc
      421 ggggcgaccc caaaaccgta tagtatgccg acacattgct gcacaatata ttaatgatat
      481 ttatcagaat gttgattaca aaccccatca agatgattat tcttcagctg aaaaatttct
      541 cactcacttc aacaaaaaat gcaaaaacca gactttggcg ttgatttcca gccgtcctga
      601 ggggcgttgc gttgctgcct gcggtgattt cgggctagtt atgaaagcat attttgacaa
      661 gatggaatca aatggcatca gtgttatggc agccatatta ctggtggata accatgcttt
      721 gacggtccgg ctaagaataa agaacacaac tgaaggatgt acccattacg tggtttcggt
      781 ttatgatcct aatgtaacta acgacaaaat aagaatcatg agcgaaagca aagaggatat
      841 taaacactat tctctgatgg attttatgaa tgtagattat agcctcctga aatggtcaaa
      901 tgatcatgtt attaaccaat ctgttgcaat aattccagca cttccgaaag aacagctatt
      961 gatattaaaa ggatc
//
LOCUS       DAZPEP010000386          974 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZPEP010000386 DAZPEP010000000
VERSION     DAZPEP010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.388"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>974
                     /gene="tssI"
                     /locus_tag="ACHUT9_004620"
     CDS             <1..>974
                     /gene="tssI"
                     /locus_tag="ACHUT9_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFR1350883.1"
                     /translation="CISQFRYSAQIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRT
                     QYEVYDYPGRFKGAHGQNFARWQMDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQAN
                     LNREWQVVASELHGEQPQAVPGRSGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQS
                     AVVTGPAGEEIFCDEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRV
                     GQEVIVDFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFD
                     DATGKEQVYIHVQKNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQ"
BASE COUNT          250 a          261 c          298 g          165 t
ORIGIN      
        1 tctgcatcag ccagttccgc tacagcgcac aaatccgtcc ttcttccgtg gtgaccaaag
       61 actacacctt taaacgaccc ggctgggcag ggcgttttga tcaggaaggc cagtaccagg
      121 attaccagcg cacacagtat gaagtgtatg actaccccgg acgtttcaag ggtgctcacg
      181 ggcagaactt tgcccgctgg cagatggatg gctggcgcaa caacgcagaa gtggcgcgcg
      241 gaacaagccg ttctccggag atatggccgg gacggcgaat tgtgctgacg gggcatccgc
      301 aggcgaacct gaaccgggaa tggcaggtgg tggcaagtga actgcacggc gagcagccgc
      361 aggcggtgcc gggacgcagt ggttcaggaa ccacgctgaa taaccacttt gcggtaatac
      421 cggcagaccg gacatggcgg ccacagccgt tgctgaaacc gctggtggat ggcccgcaga
      481 gcgccgtcgt gacgggaccg gcaggcgagg aaatcttctg tgatgaacat ggccgcgtgc
      541 gggtgaaatt taactgggac cgctataacc cgtcaaacca ggacagttca tgctggatcc
      601 gtgtggcaca ggcgtgggca ggcaccggat tcggtaacct tgcgataccg cgcgtgggtc
      661 aggaggtcat tgtggacttc ctcaacggcg atccggacca gccgattatt atggggcgta
      721 cctaccacca ggaaaaccgc acacccggta gcctgccggg gacgaagacg cagatgacca
      781 ttcgttcgaa aacctataag ggcagcgggt ttaatgaact gaagtttgac gatgcgacag
      841 ggaaagaaca ggtctacatc cacgtgcaga agaacatgaa caccgaggtg ctgaataacc
      901 gcaccacgga tgtgataaac aaccatgccg aaaaaatagg taacaaccag gcgatcaccg
      961 ttaccaataa ccag
//
LOCUS       DAZPEP010000387          973 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZPEP010000387 DAZPEP010000000
VERSION     DAZPEP010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.389"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..695)
                     /locus_tag="ACHUT9_004621"
     CDS             complement(<1..695)
                     /locus_tag="ACHUT9_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFR1350884.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          291 a          160 c          206 g          316 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac attaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagctct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatattctg
      721 ataaaatgtg atactgggaa ataataagct gaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaaga gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa cgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccg
//
LOCUS       DAZPEP010000388          970 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZPEP010000388 DAZPEP010000000
VERSION     DAZPEP010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..970
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.390"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..93
                     /locus_tag="ACHUT9_004622"
     CDS             <1..93
                     /locus_tag="ACHUT9_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFR1350885.1"
                     /translation="SHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            93..609
                     /locus_tag="ACHUT9_004623"
                     /pseudo
     CDS             93..609
                     /locus_tag="ACHUT9_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          320 a          172 c          224 g          254 t
ORIGIN      
        1 tcacatggac acaccatcac cgttaacgct gctggtaacg cggaaaacac cgtcaaaaac
       61 atcgcattta actatattgt gaggctcgca taatggcatt cagaatgagt gaacaagcac
      121 ggaccataaa aatttataac ctgctggccg gaactaatga atttattggt gaaggtgacg
      181 catatattcc gcctcataca ggtctgcctg caaacatcgt ggcatctcgt tgaagatcat
      241 cggggtaaaa cggtttatga cgtggcttcc ggcgacgcgt tatttatttc tgaactcggc
      301 tcattaccgg aaaatgtcac ctggttatcc ccggaagggg agtttcagaa gtggaacggc
      361 acagcctggg tgaaggatac ggaagcagaa aaactgttcc ggatccggga ggcggaagaa
      421 acaaaaaaca gcctgatgca ggtagccagt gagcatatag cgccgcttca ggatgctgtg
      481 gatctggaga tcgcaacgga gaaagaaacc ttgttgctgg aagcctggaa aaagtatcgg
      541 gtgttgctga accgtgttga tacatcaact gcaccggata ttgaatggcc agtagcacct
      601 atagggtaaa ttcgtaatga ttacctaaat acgttattct tttgttaaaa aagtgatttt
      661 gctataggta gggagtatgt tcttggatta agaataaaaa atcgccatga aagagcttca
      721 tggcgagtac ataaacgtac aaaatgtgat gaaagtagag tggcgtaagc cactatatta
      781 agatatgaca atagcgaaga aaagtaaata acattttatg gacgtttaat tgttaattat
      841 aaaaactaga gtgcccgata ttgtttatct tgctttaaaa taatgtactc aaaccaaatt
      901 tttgagctaa aagctcaaat aatataaatg caataataat gtttaataag acaattagct
      961 tgctttgtat
//
LOCUS       DAZPEP010000389          958 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZPEP010000389 DAZPEP010000000
VERSION     DAZPEP010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.391"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            6..958
                     /locus_tag="ACHUT9_004624"
                     /pseudo
     CDS             6..958
                     /locus_tag="ACHUT9_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
BASE COUNT          236 a          279 c          293 g          150 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggta
//
LOCUS       DAZPEP010000390          935 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZPEP010000390 DAZPEP010000000
VERSION     DAZPEP010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.392"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(197..>935)
                     /locus_tag="ACHUT9_004625"
     CDS             complement(197..>935)
                     /locus_tag="ACHUT9_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFR1350886.1"
                     /translation="GPTLTFLFVSPLLNPIIVGLMWMTFGWKVTLLYAIIAAGVSVLA
                     SIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKVSCCSPAAKAIENPVVTCC
                     STKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPF
                     AIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALIIGSAGASLTEVILLKSMF
                     RMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          279 a          198 c          199 g          259 t
ORIGIN      
        1 ttgcttctcc gataaaatgt cagagaagca tgattacgtg gttattgtct gttgatgact
       61 tgttgaccga gccgtcatca ttctcaatgc attagttaaa tcatttgtga tttttaagga
      121 ttatcctgtc tatgattttc tacttagaag gatatggcac taactatgca tcttatagtt
      181 tactgctggc atctatttaa aatagcaatt gagtcaaata gcccatcaag atagccatac
      241 ctaatataac cgtcaggaat gcagctatca tcggcattct gaacattgat ttaagtaata
      301 tcacttccgt caggcttgcg ccggcactgc cgatgattaa tgccattaat gctcccagac
      361 ccattccttt tgtcatcaga acagatgcca gagggataac cgcctcagcc cggatataca
      421 gcggaatacc gacaacggcg cttaatggga tggcgaaggg attatctgct cctgcatgag
      481 cggcaatcca cgctgaggga ataaagccat aaataaaaga tcctattaat actcctaaca
      541 aaagatatgg cagtacatct ttaaactgtt gcaacgcatc tttggtggct agttttatgg
      601 gattaatact tactacagct ttggtactgc aacaggtaac tacggggttc tcaatggctt
      661 tagccgctgg actacaacaa ctcactttta tgggcgtata tgtcgttccc ggcgaagttt
      721 ttgctggagc acaacaattt gctgatgagc ttttactggc aatgatatga cgttcaaacc
      781 ccagagaatc caggataata ctggcaagaa cggagacgcc ggctgcgata atcgcgtaca
      841 acaaggtgac tttccaacca aaggtcatcc acattagtcc gacgataatg ggattaagta
      901 atggtgaaac aaataaaaaa gtgagagtcg gacca
//
LOCUS       DAZPEP010000391          929 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZPEP010000391 DAZPEP010000000
VERSION     DAZPEP010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.393"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            75..860
                     /locus_tag="ACHUT9_004626"
     CDS             75..860
                     /locus_tag="ACHUT9_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350887.1"
                     /translation="MNLRRVCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          307 a          172 c          204 g          246 t
ORIGIN      
        1 tcaagtggag tatataatta aaaatcgtta gctaaacatt accatgatta tgacaggcga
       61 gggagtgtcc gattatgaat ttaagaaggg tttgttgtat atgtgtatta ttctcgctgc
      121 ttgcaggatg tgcctctgaa tcttctattg atgaaaagaa gaaaaaagct caagtcacac
      181 aaagtaatat taataaaaac actccccagc aactgacaga caaagattta ttcggtaatg
      241 aaaccactct ggccgtatcc gaagaggata ttcaagctgc gcttgatgga gatgagttcc
      301 gcgttcccct taattctcct gtaatacttg ttcaatccgg caaccgcgca ccggaaacca
      361 ttatgcagga ggagatgcga aaatattata ctgtttccac attctctggt atcccggaca
      421 ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa tgagaacgag gatgttgcca
      481 gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt ggctgcaaaa gggcatcaga
      541 aagcgattat tgttttccag gatacgttgc agacaggaaa atatgactct ggattgaaat
      601 caatagtttg gtcagattat aaaaatcaaa aattgactga cacgatgtca ctgcgctatc
      661 tggtacgatt cacgctggtg gatgtagcta caggtgagtg ggctgcctgg tcgccggtga
      721 attatgaata taaagtgctt ccaccattgc ccgacaagaa tgaagccagt acgactgata
      781 tgacagagca gcaaatcatg caacttaagc aaaagaccta taaagcgatg gtaaaagatt
      841 tggttaaccg ctatcaataa taaattatat ctgccgccag gaatttctgg cggaaataat
      901 acaaaattct ttgcataaga aatttttct
//
LOCUS       DAZPEP010000392          897 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZPEP010000392 DAZPEP010000000
VERSION     DAZPEP010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.394"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            44..>897
                     /locus_tag="ACHUT9_004627"
     CDS             44..>897
                     /locus_tag="ACHUT9_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1350888.1"
                     /translation="MPQLKGVIKTPTGEPLDGATITLTSIHNRAGILKSVFSHVTTQN
                     GEYDFPVLPGVYSVRLTQSTRRLSEIGVIRVYEDSTDGSLNDFLGATNIDLRPESLKK
                     FEELAQQAQQSAGSAAGNARQTAQDVTAAATARDDAQRFAEKARQDATVTAENRKATA
                     EDVKSTGKNAVLSGQRAQAAAGYARAAEQAKNDIDAALTGTLKMANHLSEIAAAGEKA
                     QQKSRDNLRLKSAATMEAQSDIYDRTKGRLAIPGAFGFGRAFLPEDVIRFDTKSDFLA
                     WVRNALPG"
BASE COUNT          244 a          237 c          257 g          159 t
ORIGIN      
        1 gataaagcca catataacgc catcaactgg ccggagcgtc ccgatgccgc agcttaaagg
       61 tgtgattaaa acgcccacgg gagaaccgct ggacggcgca accattacac taacctccat
      121 acacaaccgt gcagggattc tgaaaagtgt tttcagccat gtcaccacac agaacgggga
      181 atatgacttc cccgttctgc cgggtgtgta cagcgttcgc ctgacacaaa gcacacggcg
      241 cctttcagaa atcggtgtca tccgcgttta tgaagattca acggacggtt cgctgaatga
      301 ttttctgggc gcaactaata ttgacctgcg tccggaatcc ctgaagaaat tcgaagagct
      361 ggcgcaacag gcgcagcaga gtgccgggag cgcagccgga aacgcacggc agacggcgca
      421 ggatgtgacg gcagccgcaa cggcccgtga tgatgcacaa cgttttgcgg agaaagcccg
      481 acaggatgca accgtcacag ctgagaacag aaaggccaca gcggaagacg tgaaaagcac
      541 ggggaaaaac gccgtcttat ccggtcagcg tgcacaggct gccgcaggtt atgcccgcgc
      601 agcagaacag gccaaaaatg acattgatgc tgcgctgact ggcactctga aaatggctaa
      661 ccatctgtca gaaatcgcag cagcaggcga aaaggcacaa cagaagtccc gggataatct
      721 gaggctgaaa agtgcggcca cgatggaagc acagagcgac atttacgacc ggacaaaagg
      781 ccgtctggcg atacccggcg cattcggctt tgggcgtgct tttctgcctg aagatgttat
      841 ccgttttgac actaagagtg atttcctggc ctgggtaagg aatgcgctgc caggtga
//
LOCUS       DAZPEP010000393          888 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZPEP010000393 DAZPEP010000000
VERSION     DAZPEP010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.395"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..568)
                     /locus_tag="ACHUT9_004628"
     CDS             complement(<1..568)
                     /locus_tag="ACHUT9_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HFR1350889.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGL"
     gene            complement(568..>888)
                     /locus_tag="ACHUT9_004629"
     CDS             complement(568..>888)
                     /locus_tag="ACHUT9_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFR1350890.1"
                     /translation="AAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAGSVI
                     VTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLKQPA
                     QEAS"
BASE COUNT          297 a          210 c          193 g          188 t
ORIGIN      
        1 caagacccat taagatccct ggcaaatatc ccgccataaa tagcgatgct acagaaacgc
       61 cgccagctgt cagggaaaac acaattaaca cgtttgaggg cggtatcagt aatccagtaa
      121 tacaagatga gacgtttaca gcggtagaaa atgccggatc ataaccttgt ttggtctgaa
      181 tgggattcag tgtgccacca acggcagcag ctgccgcaac ggctgaacca gaaatggaac
      241 caaacatcat attcgccagg acgttcacat gaccaagtga accaggaacc cgaccaacca
      301 ttacctgggc gaggttaatc aaacgtatcg caataccgcc gctgttcatc agcgttccgg
      361 caaagatgaa aaaggggatt gccaataggg caaagttatc cagtccatta gccagtcgct
      421 gcgaaacagt gattgccgcg atatcccagg gaaacatcaa aagcattgaa gctacagtgg
      481 caataccgat tgagaacgaa atgggcacac caataaagac cagcacaaaa aaacagccaa
      541 acagtgtaat cgcgatataa ctatccatta actggcctcc tgcgcaggtt gtttcagttg
      601 ttgaatgctt tgattaataa aacacaaggc ataaaacatc atcaccaatc cacttaatgg
      661 cagaatgata tatacgtatc ccatcggtat ttgcatcgcg gcagatacct gtgatgttgc
      721 cagcgtttta tcaattaatt tcaggccacc agtcacaatg accgaaccag caaaacctaa
      781 aataagaacg ttaatgacta tactaagaag taattgcttg cgcttattca gggcgagagc
      841 aaataaatcg atagagagat gtctctgcgc gccgacggta tatgcagc
//
LOCUS       DAZPEP010000394          884 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZPEP010000394 DAZPEP010000000
VERSION     DAZPEP010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.396"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            462..809
                     /gene="tnpB"
                     /locus_tag="ACHUT9_004630"
     CDS             462..809
                     /gene="tnpB"
                     /locus_tag="ACHUT9_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFR1350891.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
BASE COUNT          223 a          239 c          228 g          194 t
ORIGIN      
        1 agcctgaacc gtctggtcag aatctgacga attagacaaa gtggtgtcca ccaaataagt
       61 agtgggaacc aaagtgtcag atatgcagaa aaatgtgact cccggcaggc gaaaaggctg
      121 ccctaattat cctcccgaat ttaaacagca gctcgttgct gcctcctgtg aacccgggat
      181 atccatctca aaacttgctc ttgaaaaaat ggcattaacg ccaatctgtt gttcaaatgg
      241 cgacaacaat ggcgcgaggg aaagctgcta ttaccttctt cagagagccc ccagctactt
      301 cctgtgactc tcgatgcagc tgccgaacag ccagaatcgc tcgcagagga cccgaaaacc
      361 ctcagtatca gcagtgaggt aacgttccgg cacgggacgc tccgcttcaa tggcaatgtc
      421 agcgaaaagc tcctgactct gctgatacag gaactgaagc gatgatcccg ttaccttccg
      481 ggatcaaaat ttggctggtt gccggtatca ccgatatgag aaatggcttc aacggcctgg
      541 ctgcgaaagt acagacggcg ctgaaagacg atcccatgtc cggccatgtt ttcattttcc
      601 ggggccgcag cggcagtcag gttaaactgc tgtggtccac cagtgacgga ctgtgcctcc
      661 tgaccaaacg gctggagcgt gggcgcttcg cctggccgtc agcccgtgat ggcaaagtgt
      721 tccttacaca ggcgcagctg gcgatgctgc tggaaggtat cgactggcga cagcctaagc
      781 ggctgctgac ctccctgacc atgctgtaaa tctctttatc ctggttgtca cagaataagc
      841 ccggtaaaat acgggcttat gaacgacatc tcttctgacg acat
//
LOCUS       DAZPEP010000395          877 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZPEP010000395 DAZPEP010000000
VERSION     DAZPEP010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.397"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>877
                     /gene="cadA"
                     /locus_tag="ACHUT9_004631"
     CDS             <1..>877
                     /gene="cadA"
                     /locus_tag="ACHUT9_004631"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFR1350892.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELH"
BASE COUNT          238 a          221 c          212 g          206 t
ORIGIN      
        1 caacttctca ccgatttacg aaggtaaatg cggtatgagc ggtggccgtg tagaagggaa
       61 agtgatttac gaaacccagt ccactcacaa actgctggcg gcgttctctc aggcttccat
      121 gatccacgtt aaaggtgacg taaacgaaga aacctttaac gaagcctaca tgatgcacac
      181 caccacttct cctcactacg gtatcgtggc gtccactgaa accgctgcgg cgatgatgaa
      241 aggcaatgca ggtaaacgtt tgatcaacgg ttccattgaa cgtgcgatca aattccgtaa
      301 agagatcaaa cgtctgagaa cggaatctga tggctggttc tttgatgtat ggcagccgga
      361 tcatatcgat acgactgaat gctggccgct gcgttctgac agcacctggc acggcttcaa
      421 aaacatcgat aacgagcaca tgtatcttga cccgatcaaa gtcaccctgc tgactccggg
      481 gatggaaaaa gacggcacca tgagcaactt tggtattccg gccagcatcg tggcgaaata
      541 cctcgacgaa catggcatcg ttgttgagaa aaccggtccg tataacctgc tgttcctgtt
      601 cagcatcggt atcgataaga ccaaagcact gagcctgctg cgtgctctga ctgacttcaa
      661 acgtgcgttc gacctgaacc tgcgtgtgaa aaacatgctg ccgtctctgt atcgtgaaga
      721 tcctgaattc tatgaaaaca tgcgtattca ggaactggct cagaatatcc acaaactgat
      781 tgttcaccac aatctgccgg atctgatgta tcgcgcattt gaagtgctgc cgactatggt
      841 aatgactccg tatgctgcgt tccagaaaga gctgcac
//
LOCUS       DAZPEP010000396          840 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZPEP010000396 DAZPEP010000000
VERSION     DAZPEP010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.399"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            115..831
                     /locus_tag="ACHUT9_004632"
     CDS             115..831
                     /locus_tag="ACHUT9_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFR1350893.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          276 a          143 c          175 g          246 t
ORIGIN      
        1 gaatattttt atttctaata gacgagattt ttcctgttat gatataatat gccgaattaa
       61 cacatgttaa cgatttacca gtaatgtaaa taaattttcg aggagatcat tccagtggga
      121 cgtaaatggg ccaatattgt tgctaaaaaa acggctaaag acggtgcaac gtctaaaatt
      181 tatgcaaaat tcggtgtaga aatctatgct gctgctaaac aaggtgaacc cgatccagaa
      241 ttaaacacat ctttaaaatt cgttattgaa cgtgcaaagc aggcacaagt tccaaagcac
      301 gttattgata aagcaattga taaagccaaa ggcggcggag atgaaacgtt cgtgcaggga
      361 cgttatgaag gctttggtcc taatggctca atgattatcg ccgagacatt gacttcaaat
      421 gttaaccgta cgattgctaa cgttcgcaca attttcaata aaaaaggcgg caatatcgga
      481 gcggcaggtt ctgtcagcta tatgtttgac aatgctggtg tgattgtatt taaaggatca
      541 gaccctgacc atatttttga aattttactt gaagctgaag ttgatgttcg tgatgtgact
      601 gaagaagaag gtaacattgt tatttatacc gaacctactg accttcataa aggaatcgcg
      661 gctctaaaag cagctggaat cactgagttc tcaacaacag aattagaaat gattgctcaa
      721 tctgaagttg agctttcccc agaagattta gaaatctttg aagggcttgt tgatgccctt
      781 gaagatgacg acgatgtaca aaaagtttat cataacgtcg caaatctcta attatctttt
//
LOCUS       DAZPEP010000397          832 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZPEP010000397 DAZPEP010000000
VERSION     DAZPEP010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.400"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(354..461)
                     /locus_tag="ACHUT9_004633"
     CDS             complement(354..461)
                     /locus_tag="ACHUT9_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFR1350894.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          168 a          251 c          195 g          218 t
ORIGIN      
        1 caacgtttcg cggctattct tgagttgttc aggttcaaga atggcccccg tgatgttgtc
       61 aggtgcatac ctgcaacgtg cgggggtttt ctctctccag caaccaatgc caccagggat
      121 aaagcccccg caacattgcg cctcaccgga acccttccgg cttgacgctg attctacgac
      181 tgtttttctg ttccggaatc cgtcacgtct tcactctgtg ctgcgcattc cgcagaatat
      241 tatccgcttg caaccgttgc atttttctta cgaaaaaggc cgcagagttt cacccctgcg
      301 gcctgatccg ggcgcaaatt gccattacgg cagcctgacg cccgcatggc acgttacttc
      361 cgcttgttca gccagttcac gatcatactg gcaagaatac cagcaatgac cggagccgct
      421 aaatcatgcc agaaggccat gcccagttct gcgagcgtca tatagccgcc tgtattgtaa
      481 tgacaacgtt tcgcggctat tcttgtattg tttggatgca agaatggccc ccgtggtgtt
      541 gtcaggtgca tacctgcaac gtgcgggggt tttctctctc cagcaaccaa tgccaccagg
      601 gattaagccc ccgcaacatt gcgcctcacc ggaatccttc cggcttgacg ctgattctac
      661 gactgttttt ctgttccgga atccgtctca tcttcactct gtgctgcgca ttccgcagaa
      721 tattatccgc ttgcaaccgt tgcatttttc ttacgaaaaa ggccgcagag tttcactcct
      781 gcggcctggt ccgggcgcaa attgccatta cggcagcctg acgcccacat ga
//
LOCUS       DAZPEP010000398          815 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZPEP010000398 DAZPEP010000000
VERSION     DAZPEP010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.401"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(246..>815)
                     /locus_tag="ACHUT9_004634"
     CDS             complement(246..>815)
                     /locus_tag="ACHUT9_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFR1350895.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          259 a          203 c          174 g          179 t
ORIGIN      
        1 agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat catacactaa
       61 atcagtaagt tggcagcatc acccgctttg gcttgataac cggataacaa cgtgcgcttt
      121 gttgatgccg gatgcggcgt gaattcctta tccggtctac aaaatttgca aatccaaagc
      181 attacattaa gacgtggcct gataaacata tcgcatcagg ccattttcgt tagctattgt
      241 ccacgtcagg ctttcacatc actcactgca ccgaccggaa tatcagcatg aacaggctcg
      301 ggcggtactt tgcgatattt atccagcaga tgaatctgga tcctgcgcag cgtgtcgcca
      361 ttgaggcgat agaaacggaa gtagagaatc agcgttacca tgaaaaacag agttggtaga
      421 gcaatcataa taaactgcat acctaatagg gcttgcgtag actgttcaac attcggtaca
      481 tagccaatca tccctaacac aaccgcaatg aaaaaagccg caaaggctga accgcccttc
      541 accaccatag tctgcacgga gtaagcgata ctttcacagc gtacgtgcag tttatattca
      601 ccgtaatcaa cggtatctgc caccatgatg acctgtaata cccagaaaag cgccgttccc
      661 acattcagca aaatacccgc aatcacaatg aggacgacgt tgtgatagct cattaatgcc
      721 attaacagga gaacaccaca gcttaacacc ggaagaatag atgctccggc ccataaaatg
      781 cgtcgggata atgatttaac taagcgtggg aagaa
//
LOCUS       DAZPEP010000399          812 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZPEP010000399 DAZPEP010000000
VERSION     DAZPEP010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..812
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.402"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            13..>812
                     /locus_tag="ACHUT9_004635"
     CDS             13..>812
                     /locus_tag="ACHUT9_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFR1350896.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTF
                     SDNPGDDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLK
                     FGEMGSFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFG
                     LVEGLNFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGA
                     AYSNSDRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLA"
BASE COUNT          232 a          160 c          199 g          221 t
ORIGIN      
        1 agggaataca taatgaaaag aaaagttctg gcaatgctgg tcccagcgtt attagttgct
       61 ggcgcagcaa atgcggctga aatttataat aaaaatggca ataaactgga tctgtacgga
      121 aaagttgatg ctcgtcatac tttttccgat aaccctggcg atgacggcga tgaaactatc
      181 atcgagttag gcttcaaagg tgagacccaa attacagatc agttaactgg ttatggtcaa
      241 gcactgacca aaacaaaggc tagcgacact gaaggttctg ataatactta tgtcaaactt
      301 gcttttgcag gtctgaagtt cggcgagatg ggctcctttg actatggtcg taactatggt
      361 gtgatttacg acgttgaagc ctggacagat atgcttcctg tattcggcgg cgactcctat
      421 acctggactg ataacttcat ggcaggccgt gcaaatgggg tagctactta ccgcaatagt
      481 gatttcttcg gtctggtgga aggtctgaac tttgcattgc agtatcaagg taacaacgaa
      541 ggcagcaatg caggagaaga tcaagaaggt actaaaaatg gtcacgaaga cgttcgcttc
      601 cagaacggtg atggttttgg tctttccact tcatatgact ttgacttcgg tttgagctta
      661 ggtgccgctt actcaaactc tgaccgtacc aattctcaag tagctcttgg cgggtatcac
      721 tacaatgaat acagcaaatt tgctggtggt gatacagctg aagcatggac atttggtgca
      781 aaatacgacg ccaacaatgt ttatcttgca at
//
LOCUS       DAZPEP010000400          811 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZPEP010000400 DAZPEP010000000
VERSION     DAZPEP010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.403"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..811
                     /locus_tag="ACHUT9_004636"
                     /pseudo
     CDS             1..811
                     /locus_tag="ACHUT9_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          224 a          199 c          217 g          171 t
ORIGIN      
        1 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
       61 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
      121 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcaac
      181 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
      241 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
      301 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
      361 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
      421 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
      481 cgctgtaagc agaaacgcaa gttcagagcg actacgaact cgaaccacaa tctgccagtt
      541 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
      601 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
      661 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
      721 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
      781 gatcgaggtt cacagtactg cgcatacgat t
//
LOCUS       DAZPEP010000401          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZPEP010000401 DAZPEP010000000
VERSION     DAZPEP010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.404"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            48..>803
                     /gene="gspA"
                     /locus_tag="ACHUT9_004637"
     CDS             48..>803
                     /gene="gspA"
                     /locus_tag="ACHUT9_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFR1350897.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITC"
BASE COUNT          218 a          181 c          199 g          205 t
ORIGIN      
        1 attattttta tgattaagga taatcaataa ttaaaacagg aagttctatg tctacgagaa
       61 gagaagttat tctttcctgg ttgcgtgaga aacgtcagac ctggcgtctg tgctatttgt
      121 tgggtgaggc tggaagtgga aaaacctggc tggcgcagca actgcaaaaa gataaacatc
      181 gccgtgtgat tactttaagc ctcgttgttt cctggcaagg taaggccgca tggatcgtta
      241 ccgacgataa cgcggctgaa cagggctgcc gtgacagcgc ctggacgcga gatgagatgg
      301 cggggcaatt actgcatgcg cttcatcgaa ctgatagtcg ctgccctctt attattattg
      361 aaaatgctca cctgaatcac cgccgcatac ttgatgatct ccaacgcgcg atatctctta
      421 ttcctgacgg tcaatttttg ctgatcggca gacccgatcg aaaagtcgaa cgtgatttta
      481 aaaaacaggg cattgaactt gtctcgacag gacgtctgac ggagcacgaa cttaaggcaa
      541 gcatccttga aggtcagaat attgaccaac ccgatctcct tttaacctcc agagttctga
      601 aacggatagc tttattatgt cggggcgatc gcagaaagct ggcgcttgcc ggcgaaacaa
      661 ttcgcttatt acagcaagcc gagcagacca gcgttttcac tgcaaaacaa tggcgaatga
      721 tttaccgcat attgggtgat aaccgccccc ggaaaatgca gcttgccgtg gtaatgagtg
      781 gaaccattat cgctattacc tgt
//
LOCUS       DAZPEP010000402          803 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZPEP010000402 DAZPEP010000000
VERSION     DAZPEP010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.405"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..494)
                     /locus_tag="ACHUT9_004638"
     CDS             complement(<1..494)
                     /locus_tag="ACHUT9_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350898.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWGFSTNCPGKAKANDATNPWRLKRTATAAEISQFGNTLSCEKSGSSSSSNSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAVYKNAWWVASSNC
                     PGDA"
BASE COUNT          265 a          169 c          145 g          224 t
ORIGIN      
        1 ttcgcatcac ccggacagtt agaagaggct acccaccaag catttttata taccgcacca
       61 ttaaagacga cataccaggt ctgaccgccc tgctgtttat tccaggcaac tacagaagaa
      121 ttagacggaa cagtgccctg tgctggtgta gccgaaccgc cattagccgg cgtattggat
      181 gcaggcgtat ttgaatttga agaagatgag ctgccgctct tttcgcagga aagtgtattg
      241 ccaaactgac taatttcagc agctgttgcg gtacgcttta aacgccacgg gttagttgca
      301 tcatttgctt tcgcttttcc cgggcaattt gtagaaaaac cccaccaggc attttcataa
      361 attttgccat cgaaaataac ctgatattta ttaccacctt gttggttatt ccatgcttcc
      421 attgccaatg ctggcagagc ggaacacacc agccccatac caatcataga tttagtaaat
      481 atatttaatt tcattataat cccttgtgac gtaaaaactg caaaacaaaa gtcctacgca
      541 ggaattattt acgttttacg agaacccttc aatatgaatt aaagtgaggc attaaaaata
      601 agaaaaaagc ctgacaaatg aagaatttta aaaacagaaa cattcatatt taaaatatta
      661 aattgaattg atattttaaa tatgaataat ttattcgttc tgacagtacg aataagatat
      721 gcagtcaaca aatgcaaaaa gggcgccgaa gcgccctttt caattcaaaa ctaattaacg
      781 tgtaattagc ccagaacttt agc
//
LOCUS       DAZPEP010000403          799 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZPEP010000403 DAZPEP010000000
VERSION     DAZPEP010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.406"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..741)
                     /locus_tag="ACHUT9_004639"
     CDS             complement(<1..741)
                     /locus_tag="ACHUT9_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFR1350899.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLC"
BASE COUNT          268 a          137 c          161 g          233 t
ORIGIN      
        1 acataaacag gtaaagacca taaatatggt gaaagtaaaa aagaaggaaa atacagcaat
       61 ggaaaaaatt ttatcggaga acaggaataa atctaaaaga tagattgctg aaatacagac
      121 tccactttta catgcgcgac aaaaattact gccttgatga aaaaaccaga aagggaagaa
      181 catcactgca aatacccaaa aaatagcaaa gtaataatga tcactggaag acgctgattc
      241 actttctgaa ggattagtcg gatattggtg ttgtgaagtg agttggttat tcaaagcgtt
      301 tatagctagc tctaatccct gagctaattt ttgttgttta aatgcgggta tcatgttgct
      361 acggatgata tctccagcca gagcatcggt taccttttct tccagcccat aacctacctg
      421 gatgcggaca gtacgatctg accaggcaac aatgatcagt atcccatcat tacgtttggc
      481 atctcctaaa cgccaattgt caaaaactct tgtagcatat tgttcaattg tctcgtcttt
      541 ggttgtggga acaattaata cagcaatttg cacttgggta ttattgaaaa tagattctaa
      601 cttgtgcgtc agttctttct tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg
      661 ttgctgtaat tgtgggacaa tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa
      721 cattagtatg aaatagcgca ttataaattt ctcattaaat ctggataaat aaattgacgg
      781 cggcatcggt aattttaga
//
LOCUS       DAZPEP010000404          790 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZPEP010000404 DAZPEP010000000
VERSION     DAZPEP010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.407"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            52..790
                     /locus_tag="ACHUT9_004640"
                     /pseudo
     CDS             52..790
                     /locus_tag="ACHUT9_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAZPEP010000405          789 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZPEP010000405 DAZPEP010000000
VERSION     DAZPEP010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.408"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..458)
                     /locus_tag="ACHUT9_004641"
     CDS             complement(<1..458)
                     /locus_tag="ACHUT9_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFR1350900.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIA"
     gene            complement(530..742)
                     /locus_tag="ACHUT9_004642"
     CDS             complement(530..742)
                     /locus_tag="ACHUT9_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350901.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          203 a          193 c          173 g          220 t
ORIGIN      
        1 gcgatggatt ttgcccgacg cccgcggcta ccgtccggat aaaaatgttc ggtgtaaaat
       61 ccagcctgaa tggttaccca ctcgcgaaaa gcctcaaacg actttagcaa tgccgtatcc
      121 cgggttctgc gtgtcgcaac tgtattcaga tattgctctg cggcttcgct cagagctggc
      181 gtatgttccc gacctactga ttcgcacagg taatcaacga aaccagacag cagttctcgt
      241 tcgcgaggcg tgatcgcccc accgaccgga gtccagtaat cgaatcccag ttgcaggagt
      301 ttgaaaaaac gcttgtggaa tgcgtagtta cgcacacgct taaagtctgc gtgtatccac
      361 tcacctgttt tgatttgatg cagaaaatcg caactctccg gcgtcgccgg gagaagtaat
      421 ccggaagagg tttgtttgat cagttgtata tgcgccatcg tagttctccg ctggcgcagt
      481 agaatgggtg ttcagcccgt tatgtagtat accagaatta atgccaatac taacaggatg
      541 ctctgactcg caattcatcc agcagtttat catttcccat aatgtcactt accctcatcg
      601 gtaaaaaaat tgcctttcga ccattacgat acatcattga ttttttggtt tcagggaagt
      661 aatccatttc gactataact gacaggtcat cacgacgtat gactgcgtat ttactactaa
      721 atagtttctt tattttttcc acgatgcctc caggtttata agtacaaacg gttatatcaa
      781 catagagac
//
LOCUS       DAZPEP010000406          788 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZPEP010000406 DAZPEP010000000
VERSION     DAZPEP010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.409"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..788
                     /gene="yfdF"
                     /locus_tag="ACHUT9_004643"
                     /pseudo
     CDS             1..788
                     /gene="yfdF"
                     /locus_tag="ACHUT9_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530666.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
BASE COUNT          294 a          139 c          146 g          209 t
ORIGIN      
        1 ctacttgagc ttataaaaaa cgccatacga gtaaacgacg atccttaaga tttaatatta
       61 ataattcctc agttacttat attgatattg gctccaatga tacagaccat attactattg
      121 gtatcgacaa ccaagaacca atagaattac ctgcgaacta taaagacaaa gaactcgtcc
      181 gtactatcat taatgacaac atagttgaga agactcatga tatcaataac aaggaaatga
      241 tcttcagcgc attaaaagaa atatatgatg gagatcctgg ttttatcttc gataagatat
      301 cacacaaact cagacatacg gtaacggaat ttgatgagag cgggaatagc gaaccaacgg
      361 acttatttac ctggtacggt aaagataaaa aaggcaactc tctcgctatt gtaattaaaa
      421 ataaaaacgg aaatgattac ttatctctcg gttactacga tcaggacgac taccacattc
      481 aaagaggaat tcgtattaat ggtgatagtc tcacccaata ttgtagtgaa aaagccagga
      541 atgcttcagc aatgcttcag cgtggtttga aagcagtaaa gctatcatgg cagaatcatt
      601 tgcaacaggt tccgatcatc aggttgtaaa cgagctcaac ggggaaagac tgagagaacc
      661 taacgaaatt tttaaacgtt taggtcgagc aataagatat aattttcaag tggacgatgc
      721 aaaatttaga cgcgacaatg taaaagaaat aatttctaat ttattcgcta acaaagttga
      781 cgttgacc
//
LOCUS       DAZPEP010000407          775 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZPEP010000407 DAZPEP010000000
VERSION     DAZPEP010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.410"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..343
                     /locus_tag="ACHUT9_004644"
     CDS             <1..343
                     /locus_tag="ACHUT9_004644"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFR1350902.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(405..>775)
                     /locus_tag="ACHUT9_004645"
     CDS             complement(405..>775)
                     /locus_tag="ACHUT9_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HFR1350903.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          175 g          200 t
ORIGIN      
        1 tgcagatggt catttttgtg gcaatttgcc gaatacgacc catttccatg agcaaaccgt
       61 ggagttcccg atccagggag agatggttga tattgtggcg catggtgagt tgggcggcga
      121 cttttcgtta gtgcctgaca gctacgtgac gatggggccg aaaagcatca tggcggcgaa
      181 aaacttgctg ataattgtca gtggcgcagg taaagcgcag gcactgaaaa atgtgctgca
      241 aggtccggtt accgaagatg ttccagcttc cgtgttgcag ctgcatcctt cgctgatagt
      301 aatcgctgat aaagccgctg ccgcagaatt agcgctcggt taagctgaag aaataaggcc
      361 ggatgtatga attacgctcc ggcctgttct cattatttaa ataactacga aaaatcaccc
      421 cacagtctca gcttgccgca gtggagacag cggaagagat aaccctgaca atcaccaccc
      481 ttttgcaggc atttagctag atcagaattt ctaataccga aattctcaca atcttcttca
      541 aggttggcaa attcatcgag gcgatctttt atatcattcc agcccacata gccgataaaa
      601 gcacagaaat cgccacaatg cgcaagccag aattcctgtt gccatccatg ataacctggc
      661 gtgcgttcaa ccaactcttt cagcatttca tcaggatatg tgttcttaat accggcaaat
      721 tcgtcctctt catcatactc aaattcaaca ccttctatgc tggcatcatc ctgaa
//
LOCUS       DAZPEP010000408          769 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZPEP010000408 DAZPEP010000000
VERSION     DAZPEP010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.411"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            192..>769
                     /locus_tag="ACHUT9_004646"
     CDS             192..>769
                     /locus_tag="ACHUT9_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFR1350904.1"
                     /translation="MTESSDYESVQVFIGVDVGKDTHHAVAINRSGKRLFDKALPNDE
                     NKLRSLISDLKQHGQILLVVDQPATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAG
                     EAKTDARDAAIIAEAARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIR
                     GLLTQIHPALERVLGPRLEHPAVLDLLQRYPSP"
BASE COUNT          168 a          235 c          199 g          167 t
ORIGIN      
        1 ccagccagac ccggcatgat tactgccccc agtcgtccat gatccggggg gtgatgtcac
       61 cgggtctggt ggggcgctgg taaccgctaa taggggtcag gtcaggcact tttgccggga
      121 ccgtctgtaa cgtggatgcc ggtacctgct ccccgtggtt atctggttaa cccatataca
      181 agggagacag aatgaccgaa tccagcgatt acgaatccgt ccaggtcttt atcggcgttg
      241 atgtcggtaa agatacgcat cacgctgttg ccattaatcg ttcaggtaaa cgcctgttcg
      301 ataaagcatt acccaacgac gaaaacaaac tcaggtcgct aatatctgac ctgaaacaac
      361 atggtcagat actgctggtt gttgatcagc cagctaccat cggtgcgtta cctgtcgccg
      421 ttgcccgctc agaaggagtc cttgtcggat acctccctgg actggccatg cgccgcatag
      481 ccgacttaca cgccggtgaa gctaaaactg atgctcgtga cgctgccatc attgccgaag
      541 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
      601 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      661 accgtatccg cggccttctg acccagatac atccggcact ggagcgcgtt ctcggtccga
      721 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacc
//
LOCUS       DAZPEP010000409          745 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZPEP010000409 DAZPEP010000000
VERSION     DAZPEP010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..745
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.412"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            63..>745
                     /gene="istB"
                     /locus_tag="ACHUT9_004647"
     CDS             63..>745
                     /gene="istB"
                     /locus_tag="ACHUT9_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005149187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family helper ATPase
                     IstB"
                     /protein_id="HFR1350905.1"
                     /translation="MSDNLLNKLTQLKLPAMAGSLIRQRETPQTYDELSFEERLTLLV
                     DDELLSRENSRVARLRKNACLKYQATPEGLRYPASRGLRAEQMRELLNGHYIIHRKNL
                     LITGPTGCGKSWIANALGEQACRQKYSVRYCRTGRLLEQLAQGRVDGSWLKYLKQLQK
                     IQVLILDDLGLEQLSNAQCNDLLEITEDRYGQSSTIVVSQFPVDKWHGLMENPTTADA
                     ILDRLVHNS"
BASE COUNT          224 a          171 c          209 g          141 t
ORIGIN      
        1 tgcttcagag catgaaaacc tgcgtggtcc tggctactac cactgacaga aggaacaacc
       61 gtatgagtga taatctgtta aacaaactga cccaactgaa actcccggcg atggccggga
      121 gcctgatacg gcaacgggaa acgcctcaga cttacgacga actgtcgttc gaggaaaggc
      181 tgacactgct ggttgatgac gagcttctga gcagagaaaa cagtcgtgtg gcacggttga
      241 gaaaaaatgc ctgcctgaaa tatcaggcaa caccggaagg gctgcgttat ccggcctcaa
      301 gaggtctgcg ggctgaacag atgagggaac tgctgaacgg ccactacata atccacagga
      361 aaaacctact gataaccggt ccgacaggat gcggtaagag ctggatagcc aatgcgctgg
      421 gcgaacaggc atgtcgacag aaatacagcg tccggtactg tagaaccggg cgattactgg
      481 aacagttggc tcagggacgt gttgatggca gttggctgaa atatctgaaa cagttgcaaa
      541 aaatacaggt tctaatcctg gacgatctgg ggctggaaca gttaagtaac gcccagtgta
      601 atgaccttct ggagatcaca gaggatcgtt acggacaaag cagcacgata gtggtaagcc
      661 agttcccggt agataaatgg catggtctga tggagaaccc aacaacagca gatgccatcc
      721 tggacagact ggtacataac tcaca
//
LOCUS       DAZPEP010000410          743 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZPEP010000410 DAZPEP010000000
VERSION     DAZPEP010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.413"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..396)
                     /locus_tag="ACHUT9_004648"
     CDS             complement(<1..396)
                     /locus_tag="ACHUT9_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFR1350906.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRL"
BASE COUNT          233 a          173 c          108 g          229 t
ORIGIN      
        1 caacctggac catgctaaat tcacaccgtg ctgcgcggcc tgattaaata gtgcatacgc
       61 tttggcataa tccacccgca cctgctcacc gtcgtagtag atacccccca gcgctaacgg
      121 agcttctctg tcaccttgtt tagccgctaa ctcgaaccat ttaatcgctt cctgaacatc
      181 aggtttggta tcataacgag gctgataata ttcccagcct aacaggctct gcgcgtcgat
      241 atctcccgat tcagccattt tatgcagtgt atttataaca tcgggagcag gtttaccacc
      301 gtatatgctg tcatacaatt catgcgcttt ctcgctggcc accacgcttg gtgaaactat
      361 cagcattaca gaaaataata ttcttttagt taacatgtat acctttatgt cctcactgtc
      421 atatatttcc ataataatta accaccaaat atccatttaa tgaataaata cagtgccata
      481 aaggtccaag taataagcac acatttagcc tgaaacatta tcatccatgt caccaaatcg
      541 tttatttagt gagataaaac tagcatattc gtaagataaa aaatgcacat ggcaatcaag
      601 aaatgatatt attacttttt tgatgtaaaa ctatttagtt ttaaaattca ttttttcaac
      661 atatagagca ttcattttat gaatgcaaaa aaacatacct gacacacaaa ttactctcct
      721 ctgtaatatt ttctcattaa cac
//
LOCUS       DAZPEP010000411          738 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZPEP010000411 DAZPEP010000000
VERSION     DAZPEP010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.414"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..78)
                     /locus_tag="ACHUT9_004649"
     CDS             complement(<1..78)
                     /locus_tag="ACHUT9_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350907.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNA"
     gene            complement(88..>738)
                     /locus_tag="ACHUT9_004650"
     CDS             complement(88..>738)
                     /locus_tag="ACHUT9_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350908.1"
                     /translation="DTLAWLVNASKLMTEFDDNGKIIFNINALKYTKASYFEILGEKY
                     IKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIWFSVAWRTIE
                     FIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVILVGGAILLT
                     GIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT          239 a          153 c          121 g          225 t
ORIGIN      
        1 agcattgaat gcagggaaaa ataatatggc cataaaaaac atcgaaagaa actcttttaa
       61 tttaacatgt aaacgcatgg ttaatcctca tatcacgggt ggagtgttaa gaacatacat
      121 aaatggagtc atgttttccc ttttccattt atcaagttcc tgttgccgtt ttagtccatc
      181 tctaattgca tattttaatt tttctgataa atggcattga gcatcgattt catttaaaac
      241 aactgtacac actatccctg tcagtaaaat tgctcctccg acaagaatta ctggaaagcc
      301 aaagctaaca caggcaaaag aagccaggct tccaatagcg gttgcaacac caccggctat
      361 aactgctttg gccacatcca ttgaaaaatc gccaagaaaa ttaaccagat ctcgctctga
      421 actcataata aattctatgg ttctccaggc tactgaaaac cagatacaga acttaactcc
      481 ttttattgat tcagataaag ctcctcgcca gccaatagcg agttccagaa cttgaggcgc
      541 tcgtgaagag aaaatatatt taccgagctt ttctaaaagc caggggctgg atgttgtgat
      601 ttttatatat ttctctccaa gtatttcaaa gtaacttgct tttgtatact ttaaggcatt
      661 aatgttaaat ataattttcc cattgtcatc aaactcagtc attaatttac tcgcgttaac
      721 taaccaggcg agtgtatc
//
LOCUS       DAZPEP010000412          736 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZPEP010000412 DAZPEP010000000
VERSION     DAZPEP010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..736
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.415"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(1..736)
                     /locus_tag="ACHUT9_004651"
                     /pseudo
     CDS             complement(1..736)
                     /locus_tag="ACHUT9_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          195 a          195 c          158 g          188 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccgacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg ataggcaaat ggtgtattgc cagtgacatc ataaccagca
      661 ccgtgcagac caataccata agtatacgca ggcgcatacc agccaaactg cggaccattt
      721 accatgattg ctttcg
//
LOCUS       DAZPEP010000413          729 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZPEP010000413 DAZPEP010000000
VERSION     DAZPEP010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.416"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..443
                     /locus_tag="ACHUT9_004652"
     CDS             <1..443
                     /locus_tag="ACHUT9_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFR1350909.1"
                     /translation="VSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
                     DQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGY
                     TANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(483..>729)
                     /locus_tag="ACHUT9_004653"
     CDS             complement(483..>729)
                     /locus_tag="ACHUT9_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HFR1350910.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          190 a          205 c          195 g          139 t
ORIGIN      
        1 ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc aacatgtcaa
       61 agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa tcaggtttaa
      121 accgttcgat actggatcag ggctggtatg aaatgcaccg ccagcttgag tacaagcagc
      181 tctggcgtgg cggtcaggtg cttgctgttc cgccagcgta tacaagccag cgttgcgcgt
      241 actgtggtca tacagcgaaa gagaaccgcc tgtcacaaag taaattcaga tgccaggtat
      301 gtggatatac agcgaacgcc gatgtaaatg gtgctcgtaa cattttagcg gcggggcacg
      361 ccgttcttgc ctgtggagag atggtgcagt caggccgccc gttgaagcag gaacccaccg
      421 aaatgattca ggcgacagcc tgaacgtagc agggatccac gtccttcagg gcgtggagga
      481 tgtcaatatc ggtttctgtc agcgattaaa ttcaacacca cgtaacaaac gcctccggca
      541 attaatcccc agaacgcaga accaatcccg accagcgtca atccacttgc cgttaccaga
      601 aatgccacca ccgccgcgtc gcgctcacgc tcacgctcat tatgcagcgc ctgatacaaa
      661 ctgccgccaa tggtactcaa cagcgccaga cctgccagca tctggatcca acttacgggc
      721 agggcagcc
//
LOCUS       DAZPEP010000414          726 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZPEP010000414 DAZPEP010000000
VERSION     DAZPEP010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.417"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            35..>726
                     /locus_tag="ACHUT9_004654"
     CDS             join(35..284,284..>726)
                     /locus_tag="ACHUT9_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
                     /protein_id="HFR1350911.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDSLRKTVVAHVFGERTMATLGRLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHY"
BASE COUNT          167 a          183 c          205 g          171 t
ORIGIN      
        1 atttagtgta tgatggtgtt tttgaggtgc tccagtggct tctgtttcta tcagctgtcc
       61 ctcctgttca gctactgacg gggtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcactgcc gtaaaacatg gcaactgcag ttcacttaca ccgcttctca
      181 acccggtacg caccagaaaa tcattgatat ggccatgaat ggcgttggat gccgggcaac
      241 tgcacgcatt atgggcgttg gcctcaacac gattttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acagccgggc agtgacgtca tcgtctgcgc ggaaatggac
      361 gaacagtggg gctatgtcgg ggctaaatcg cgccagcgct ggctgtttta cgcgtatgac
      421 agtctccgga agacggttgt tgcgcacgta ttcggtgaac gcactatggc gacgctgggg
      481 cgtcttatga gcctgctgtc accctttgac gtggtgatat ggatgacgga tggctggccg
      541 ctgtatgaat cccgcctgaa gggaaagctg cacgtaatca gcaagcgata tacgcagcga
      601 attgagcggc ataacctgaa tctgaggcag cacctggcac ggctgggacg gaagtcgctg
      661 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
      721 cactat
//
LOCUS       DAZPEP010000415          709 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZPEP010000415 DAZPEP010000000
VERSION     DAZPEP010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.418"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..87
                     /locus_tag="ACHUT9_004655"
     CDS             <1..87
                     /locus_tag="ACHUT9_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFR1350912.1"
                     /translation="RMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            102..524
                     /locus_tag="ACHUT9_004656"
     CDS             102..524
                     /locus_tag="ACHUT9_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1350913.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            525..>709
                     /locus_tag="ACHUT9_004657"
     CDS             525..>709
                     /locus_tag="ACHUT9_004657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350914.1"
                     /translation="MKLAIRLQLPLAFASKERSMEIKPEDELSNIVLFPVKEDDPRNQ
                     VNFLYEPSERPYCHHASV"
BASE COUNT          189 a          145 c          198 g          177 t
ORIGIN      
        1 cgcatggtca tgaacggcgg gcgctgggtg aattttaact gggagagctg gcgtccgaat
       61 gttagccatt cgagggttgt taagtagttt caggaggatt tatggcgaaa ccttttactc
      121 ccgaacagcg ggaagaactg aagacgcgaa ttgtggaact cgtgcatcag gacggtcggg
      181 tcacgattcg gcagttgtca gatgaaacag gtatcagtcg tgcgtctgtc ggtcgcttat
      241 gcatagaact ggtcgcaagt ggtgatgtat ataattctgg ctacggctta ttcccgtctg
      301 aacaggctcg caaggactgg caaagcgccc gcaaaaaact ctcgagagta aaggtgagga
      361 aaccggttgt tgttgatccg gaccttatct ggtcattacc tgacggagaa atacgccgct
      421 acgacaggcg cctgaatata atctgtcgcg agtgccggaa gagcgaagct atgcagcgtg
      481 tactggcttt ctatcagggt aattttcagg aggcgatact gtgaatgaaa ttagctatca
      541 ggcttcaatt accgctggca ttcgcatcaa aggagaggag catggaaata aaaccagaag
      601 atgagttaag caatatcgtt ttatttccgg taaaagagga tgaccctcgt aatcaggtta
      661 attttcttta tgagccatcg gaaagaccat attgccatca cgcctctgt
//
LOCUS       DAZPEP010000416          707 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZPEP010000416 DAZPEP010000000
VERSION     DAZPEP010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.419"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            282..686
                     /gene="stpA"
                     /locus_tag="ACHUT9_004658"
     CDS             282..686
                     /gene="stpA"
                     /locus_tag="ACHUT9_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFR1350915.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT          213 a          140 c          158 g          196 t
ORIGIN      
        1 tcttttattc tgttatattg catttttatt cacttcggtt ttgtcaggct tgcggaatta
       61 gcgagcagag agcgccgttc tgttcaccaa taatctaaat tactcatcaa aatggctaat
      121 atataatctt gaaattattc tgagagtttc agaaaataac agctgaaata atctcgcgca
      181 agactgtaaa tagattaaat tttgtggaaa tataataagt gatcgcttac actacgcgac
      241 gaaatacttt tttgttttgg cgttaaaagg ttttctttat tatgtccgta atgttacaaa
      301 gtttaaataa cattcgcacc ctccgtgcga tggctcgcga attctccatt gacgttcttg
      361 aagaaatgct cgaaaaattc agggttgtca ctaaagaaag acgtgaagaa gaagaacagc
      421 agcagcgtga actggctgag cgtcaggaaa aaattagcac ctggctggag ctgatgaagg
      481 ctgacggaat taacccggaa gagttattgg gtaatagctc tgctgctgca ccacgcgctg
      541 gtaaaaaacg ccagccgcgt ccggcgaaat ataaattcac cgatgttaac ggtgaaacta
      601 aaacctggac cggtcagggc cgtacaccga agccaattgc tcaggcgctg gcagaaggta
      661 aatctctcga cgatttcctg atctaattac tgaggccgga tgtcgct
//
LOCUS       DAZPEP010000417          705 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZPEP010000417 DAZPEP010000000
VERSION     DAZPEP010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.420"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..213
                     /locus_tag="ACHUT9_004659"
     CDS             <1..213
                     /locus_tag="ACHUT9_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HFR1350916.1"
                     /translation="STSKTPSYTKSVSWQHHPLTIPCKSDPDLCMQLDAWDAETSIPA
                     LLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            318..509
                     /locus_tag="ACHUT9_004660"
     CDS             318..509
                     /locus_tag="ACHUT9_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFR1350917.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(510..>705)
                     /locus_tag="ACHUT9_004661"
     CDS             complement(510..>705)
                     /locus_tag="ACHUT9_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HFR1350918.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
BASE COUNT          179 a          179 c          154 g          193 t
ORIGIN      
        1 agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca cccgttaacc
       61 attccttgta aatctgaccc cgatttatgt atgcaactcg atgcctggga tgctgaaacc
      121 agtatccctg ccctgctaaa tggcgaacac tctgtccttt accgtacccg ttacgatcaa
      181 caatctgatg cctggattat gcgtcttgcc tgatccaaaa agaacccgtc gggatggcgg
      241 gttatttgtc ctggttattc ccccgttgta aaatctctcc taaacttaac ggtacggcac
      301 cacacttcgg ggatgaaatg ttcgcgctgg tactttttgt ttgctacctg gatggcggtt
      361 gtgaagatat tgttgtggat gtctacaaca cggaacagca gtgtctttat tctatgagcg
      421 atcaacggat ccgccatggc ggttgttttc cgattgagga ttttatagat ggtttctggc
      481 gacctgcaca ggagtacggt gatttttaat tattgcaatt gcacaagagt cagttcgccc
      541 ccaaagacag caccggtatc aatataatgc aggttgccaa tatccacgcg atgtcgcaac
      601 ggtgtatgac caaaccagaa atgatcagca cctgtaattc cctgcccttt ttggcgttca
      661 cctaatcgcg agcggctcca caagacctga tgcaaatcaa cgtcc
//
LOCUS       DAZPEP010000418          702 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZPEP010000418 DAZPEP010000000
VERSION     DAZPEP010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.421"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>702
                     /locus_tag="ACHUT9_004662"
     CDS             <1..>702
                     /locus_tag="ACHUT9_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFR1350919.1"
                     /translation="SRHTDDTDVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAI
                     NAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLR
                     VTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCY
                     RANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLA
                     EAFEHYNEWHPHSALG"
BASE COUNT          172 a          169 c          216 g          145 t
ORIGIN      
        1 cagtcgtcac actgatgata cggatgtgct tctccgtata caccatgtta tcggagagct
       61 gcccacgtat ggttatcgtc gggtatgggc gctgcttcgc agacaggcag aacttgatgg
      121 tatgcctgcg atcaatgcca aacgtgttta ccggatcatg cgccagaatg cgctgttgct
      181 tgagcgaaaa cctgctgtac cgccatcgaa acgggcacat acaggcagag tggccgtgaa
      241 agaaagcaat cagcgatggt gctctgacgg gttcgagttc tgctgtgata acggagagag
      301 actgcgtgtc acgttcgcgc tggactgctg tgatcgtgag gcactgcact gggcggtgac
      361 taccggcggc ttcaacagtg aaacagtaca ggacgtcatg ctgggagcgg tggaacgccg
      421 cttcggcaac gatcttccgt cgtctccagt ggagtggctg acggataatg gttcatgcta
      481 ccgggctaat gaaacacgcc agttcgcccg gatgttggga cttgaaccga agaacacggc
      541 ggtgcggagt ccggagagta acggaatagc agagagcttc gtgaaaacga taaagcgtga
      601 ctacatcagt atcatgccca aaccagacgg gttaacggca gcaaagaacc ttgcagaggc
      661 gttcgagcat tataacgaat ggcatccgca tagtgcgctg gg
//
LOCUS       DAZPEP010000419          701 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZPEP010000419 DAZPEP010000000
VERSION     DAZPEP010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.422"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            132..>701
                     /locus_tag="ACHUT9_004663"
     CDS             132..>701
                     /locus_tag="ACHUT9_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1350920.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELD"
BASE COUNT          196 a          159 c          196 g          150 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga c
//
LOCUS       DAZPEP010000420          698 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZPEP010000420 DAZPEP010000000
VERSION     DAZPEP010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.423"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>698
                     /locus_tag="ACHUT9_004664"
     CDS             <1..>698
                     /locus_tag="ACHUT9_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350921.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGI"
BASE COUNT          238 a          138 c          182 g          140 t
ORIGIN      
        1 attgctcaac agaaagctaa aacagaaaag cagaatcaaa gagcacaagc tgccgcagca
       61 agagcacagg caaggcaaca gcaacaaata gcagcacgta aagagaaagc agaattaaaa
      121 ctccttgatg tatcaagctc tatcagtgca atgcaccgtc tctctgtagc ggaacaatac
      181 aaagctattg cacaggctag agagatagca aaacaatatg agcaaggggc tatcagcctg
      241 gcgcgtatga atagtcagat gaaacgccta caacagcaac aacgtaagat taatggcaac
      301 agaaaagctc aatttgcccc cgttaaggga ggatcaggga attccgccac gatgggcgct
      361 ctgttatttg gaggcgctac ggctgctgct ggcgtgatgg ctgttagtaa aacctctgaa
      421 tttatggcta acagctttgc taatgctgaa acagaagggg agctaatcca acgtgcaagg
      481 cttggtggag ttgattacaa cttacttaat aatcttgagc gttgggcgta tgacaacggg
      541 atcgatagcc tccagggtga tccgggacgc agaaaaatac tagatgccat cggtaaagat
      601 gtaaaggaac gcgctaccaa aagctatgac gaggctgaat atgtagttga taagaaaaca
      661 gggaaaggca tatggaaggg cggtgattct ggtattaa
//
LOCUS       DAZPEP010000421          695 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZPEP010000421 DAZPEP010000000
VERSION     DAZPEP010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.424"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>695
                     /locus_tag="ACHUT9_004665"
     CDS             <1..>695
                     /locus_tag="ACHUT9_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFR1350922.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEVY"
BASE COUNT          162 a          167 c          205 g          161 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg tacat
//
LOCUS       DAZPEP010000422          693 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZPEP010000422 DAZPEP010000000
VERSION     DAZPEP010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.425"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>693
                     /locus_tag="ACHUT9_004666"
     CDS             <1..>693
                     /locus_tag="ACHUT9_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFR1350923.1"
                     /translation="PTSQFWSYATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLT
                     EIIFKDEFAAVIPDNVSTQAACAKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLL
                     TLLRGETKYGSLKYLDQDALNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTIT
                     DKTVLIHYTGITKPWHSWAGYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLF
                     AHGEYIKGITSL"
BASE COUNT          215 a          135 c          139 g          204 t
ORIGIN      
        1 gccgacgtca cagttttggt cgtacgcgac atacttcagg gtattgtcct ttgaatatct
       61 gagtgaaagt atttccacac tgctgtatct ggatgccgat gtcgtttgta aaggaagtct
      121 gaagcctctc acagaaatta tatttaaaga tgagtttgct gcggtcattc ctgacaatgt
      181 tagtactcag gcggcatgtg caaaacgcct caacattccc gagatgaatg gacgttattt
      241 caatgcaggc gttatctatg tcaatcttaa aaaatggcat gaagcaaatt tgacaccgta
      301 tttactcacg cttttacgag gggaaactaa atatggctct cttaaatatt tagatcagga
      361 tgcgttgaat atcgcattta atatgaataa tatctacctc gcgaaggatt ttgatactat
      421 ttataccctg aaaaacgaac ttcatgatcg tagtcatcga aagtatcagc aaaccattac
      481 cgataaaaca gtattgattc actatacagg gataactaaa ccatggcata gctgggctgg
      541 ttatccgtct gcatcatact ttaatatcgc gcgtgaacaa tctccctgga agaaatatcc
      601 tcttaaagag gcgcggactg ttgcagaaat gcagaaacaa tataagcatc tgtttgccca
      661 tggtgagtat attaaaggca taacttcatt aat
//
LOCUS       DAZPEP010000423          690 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZPEP010000423 DAZPEP010000000
VERSION     DAZPEP010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.426"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
BASE COUNT          241 a          105 c          143 g          201 t
ORIGIN      
        1 agtctatttt ataaaaaaat taagtaggct aagaagtacc ttagaaatca attttaaggg
       61 gtctaaaacg ctctgtaacg agctaaactg tataagtggc ataagatgcc ttttagactg
      121 acactcgctt agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac
      181 agagacacag tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac
      241 taagataaga agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta
      301 atagactgtc ttaatagata acttaaaagt cttaaaggat atcttccaag aagagaggaa
      361 cgaagaacca gaggaagtga ccgttgaccg tttgaacaga ctgtttaagt aaagcctata
      421 aagtatctat taattaaggg ctatgtctca atactagcag tttgtactag cagtttgtca
      481 atacctttgt ctttagcaat tcgtgctatt tattaaatat agctgttaaa acagagtatg
      541 ctttttaacc taaaaccttg tcgcttcgct ctgggtcaca tgttcttggg ttcttagtga
      601 gagttacact atcttctaag gtactcaata aaaagacgag taacaaccga agaacgagga
      661 tagaggtatt gtaataactt agaaggtgtc
//
LOCUS       DAZPEP010000424          679 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZPEP010000424 DAZPEP010000000
VERSION     DAZPEP010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.427"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>679
                     /locus_tag="ACHUT9_004667"
     CDS             <1..>679
                     /locus_tag="ACHUT9_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFR1350924.1"
                     /translation="QMQSQMDPVYTPARKIHLYHCDHRGLPLALISTEGTTAWCAEYD
                     EWGNLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPVQYIDSMGLASKYGHLNNGGYGAKPSKPPTPDPSKLPDIAKQLRLPYPI
                     DQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCMPQDDPQYPGMETKTASDYLPQ
                     TNWSTTQL"
BASE COUNT          195 a          170 c          171 g          143 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaatta
//
LOCUS       DAZPEP010000425          670 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZPEP010000425 DAZPEP010000000
VERSION     DAZPEP010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.428"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(301..507)
                     /locus_tag="ACHUT9_004668"
     CDS             complement(301..507)
                     /locus_tag="ACHUT9_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFR1350925.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
BASE COUNT          179 a          156 c          130 g          205 t
ORIGIN      
        1 acaatgctac cgccatactg cgtcatgtcg ccctgacgcg ccgccggttt tccgctcatt
       61 tctcatcctt gttttatcgg gttatttgtc cgctgacagg caagacccta cagatttata
      121 agaaagcctt aagtgagata tccgtcagat agaaacaatt aggaaaatat ttatatatat
      181 ttcatgaata tatgtttcga ttggtataac ggtgtctaaa tattcctctg aacatcttcc
      241 tgatacaaaa aaccgccagc gtttccgctg gcggttttca atgtattcag aactcagcaa
      301 tcagtaaaaa tcaccggttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt
      361 tttcgaccag acgcggtgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat
      421 gaaaggcagc gccagaacgc ccgctgcaac cgcatatgta cggcgtaaaa agataagcca
      481 tgcaggatat tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa
      541 tagcaccgga aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt
      601 tactactcat ccgaccactt atttttgctt attgatggtt tatttacatt cactctgtaa
      661 ttaagttaca
//
LOCUS       DAZPEP010000426          663 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZPEP010000426 DAZPEP010000000
VERSION     DAZPEP010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.429"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>663
                     /locus_tag="ACHUT9_004669"
     CDS             <1..>663
                     /locus_tag="ACHUT9_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1350926.1"
                     /translation="TFIADDNNAELIPCLSTDLLVSLGIKKIALLDNKEHSAEKHVPD
                     NSACTPLQDRLADASTEFDVGQQHLSLSVPQIYVGRMARGYVSPDLWEEGINAGLLNY
                     SFNGNSINNRSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSDSNKWQ
                     HINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDRQHGFAPVIH
                     GI"
BASE COUNT          201 a          128 c          145 g          189 t
ORIGIN      
        1 ttacttttat tgcagatgat aataacgcag aactcatccc atgcctgagt acagatcttc
       61 ttgtcagtct tggtataaaa aaaatagcgt tattagacaa taaagaacat tcggcagaaa
      121 aacatgtgcc ggacaacagc gcatgcacac cacttcagga tcgtttggcc gacgcatcaa
      181 ctgagtttga tgttggtcag caacatctgt cactctccgt tccgcaaatt tatgttggca
      241 gaatggctcg cggctatgtt tctcctgatc tgtgggaaga aggaataaat gcagggcttc
      301 taaactatag ttttaatggt aattctatta ataatcgtag taaccataat gcaggaaaat
      361 caaactatgc atatttgaat ttacagagtg gcatcaacat tggtagttgg cgactacgcg
      421 ataactcaac gtggagttat aacagtggga gtagcaattc atctgacagc aataaatggc
      481 agcatatcaa tacgtcggct gaacgtgaca ttattccctt acgctcacgg ttaacggtag
      541 gtgatagtta taccgatggc gatatttttg atagtgtgaa ctttcgtggc ctcaaaataa
      601 attcaacaga agcgatgttg cccgatagac aacatggttt cgctccggtg attcatggta
      661 ttg
//
LOCUS       DAZPEP010000427          649 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZPEP010000427 DAZPEP010000000
VERSION     DAZPEP010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.430"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(26..330)
                     /locus_tag="ACHUT9_004670"
                     /pseudo
     CDS             complement(26..330)
                     /locus_tag="ACHUT9_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            384..587
                     /locus_tag="ACHUT9_004671"
                     /pseudo
     CDS             384..587
                     /locus_tag="ACHUT9_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558123.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          158 a          175 c          153 g          163 t
ORIGIN      
        1 gcatcatttt ttccagaggc ggtcaaactc cgccttcgca aaataagcgg aagcctggcg
       61 aaggatatcg ttactgcggc gcagttcacg attttcacgc tccagctctt tcagacgctg
      121 acgttcagcg gtggtgagcc caccatcacc gcccccggta tcccgctcat gctggcgaac
      181 ccagacacgc agagtctccg gcgtacagcc aatctttggg gcaatggaac aaattgccgc
      241 ccactgtgag tcatattcat cctgactttc cagaaccata cgaatcgccc gctgacggac
      301 ttcgggggaa aaacgagtat ttttagtcat cctgtttacc tctttctcag ggagtttagt
      361 ctccaggatt tccggggcgg ttcagacatc tagaatttac tgtcagcacc ggcgttaaag
      421 tttacttctg cgatcctcag agtccttggc agcggggaac aaatgagaat acaaatgggc
      481 taattcggca gtactttcct aaaaagacat gtcttgccca atatactcaa catcgagtag
      541 gcagcctgac ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt
      601 cattgtcttt caggtggtta tatggcccgt tcagctaaac cccgtaaac
//
LOCUS       DAZPEP010000428          647 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZPEP010000428 DAZPEP010000000
VERSION     DAZPEP010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.431"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..248
                     /gene="tnpA"
                     /locus_tag="ACHUT9_004672"
                     /pseudo
     CDS             1..248
                     /gene="tnpA"
                     /locus_tag="ACHUT9_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          212 a          146 c          113 g          176 t
ORIGIN      
        1 aaatggatgg cgaaccagat cacgtccatt tgttaattaa ctatcctccc aaactggcga
       61 tatccagtct ggtaaacagc ctcaaaggcg tatcgggtag gtgactgcga cgagatcgac
      121 cagatattgc agtcaggtat tactacaaag gcgttttgtg gagtgttggc tattttgcca
      181 gtagctgcgg aggtgcgcca atatccgtca tccgccaata cattgaacaa cagcaaacac
      241 ctggttaggt ggaaaaccac gccttatatc cctgccctga aggacggggt tttacggcgc
      301 accggataag cctggagatt atctacacca gcatggacat cattgtacta tgccccgtac
      361 atgccccatc acatcctcaa catcaaagta ttagagcctt gcaaaccatt atgcgtctca
      421 gtttgtctca tctatcttac gcaaaaaaaa tagaactaat atcaagcggc gctatattgc
      481 gtattaaata aaaccacctc aattacatta ataaatctaa acaaacatca tgcaaataat
      541 ttccatggtt atttctaggc tatttaaatt aaataaataa attatgagtc aatcataaat
      601 aacaaccttt aatataattt aactaccaaa tttatattcg ttcacaa
//
LOCUS       DAZPEP010000429          644 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZPEP010000429 DAZPEP010000000
VERSION     DAZPEP010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.432"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>644)
                     /locus_tag="ACHUT9_004673"
     CDS             complement(<1..>644)
                     /locus_tag="ACHUT9_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350927.1"
                     /translation="RNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFTD
                     CSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDL
                     TAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYDP
                     VDRLTEQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPA"
BASE COUNT          107 a          211 c          179 g          147 t
ORIGIN      
        1 gccggttcac cgttcaccgt gcgccgggtg atacggtccg acgcatcgta atgccagagg
       61 gtgataagcc cctcatcttc actctgcgtg agttttccgg tcaggtcata ctggtaacgt
      121 tgcgtccggc cgtcaaagcc gcgctgctcc gtcagcctgt cgaccgggtc ataccggaac
      181 gtggactggc tgccgttctc gttggtcagc acagtgatgc gcccggcggc gtcatacccc
      241 atgcttcgcg tcagaccgcc ctgcgtggtg ctgacggcct ttccccacgc atcatactgt
      301 atctcactgc ggctgccgtc cggggcaacg atagcggtga ggtctcctgc ggcgctgtac
      361 tcataacggg tttcacgccc ctgcgcatcc ctctgactga ccagttgacc gcgcgggtta
      421 taactgctgt aagtgctgat gccttcttcc cggtgaacgg cgatttgttg accgtaccgg
      481 tcatactcat accgcgtcgt gtaccccgag cagtccgtaa acgcctgaag ctgaccgtac
      541 cggtttcgcg ttattctccg cctcaccccg gttccgtctt ccaccgcaca gggcaggcca
      601 ctgcgggaag aatcataaaa ccagcgcgta atgttaccgt tgcg
//
LOCUS       DAZPEP010000430          643 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZPEP010000430 DAZPEP010000000
VERSION     DAZPEP010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.433"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>643
                     /gene="fliD"
                     /locus_tag="ACHUT9_004674"
     CDS             <1..>643
                     /gene="fliD"
                     /locus_tag="ACHUT9_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFR1350928.1"
                     /translation="KVSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDE
                     SSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLV
                     LTASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSS
                     NKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          219 a          146 c          139 g          139 t
ORIGIN      
        1 aaagtttcca ccgaagcagg tgctgccccc ggtatctata ccatcagcgt aacccaactg
       61 gcgcaggcgc aatctttacg aactgattcc ccgacaatca ttgcatcaac aaaagatgca
      121 ctgggtgatg agtcatcaga tacacgcaca ataaaaatta cccaagacgg tcgtaaagag
      181 ccgctggaaa tcaagctcaa taaagatcag acgtcattgg atgagatcag caaagccatt
      241 aacgatgccg atagtggaat ttccgctagt atcgttaaag tcaaagatgg taactatcaa
      301 ttggtattga ccgccagtga aggtctggcc aataaaatga ccatatctgt tgagggtgac
      361 agtaaattaa atgatctgct ggcttatgac agcaagacca acaccgggaa tatgaaggaa
      421 ctggttaacg cgcaaaatgc acagctaaac gttaacggta tcgatattga acgttcaagt
      481 aacaaaatta ccgatgctcc tcagggagtg actctcgatt tgaccaaaaa agtcactgac
      541 gtccgggtca cggtgacaaa aagcaacgat aaagcaaccg aagcaattaa aggctgggtg
      601 gattcatata actccctcat tgacaccttt aacaccctga caa
//
LOCUS       DAZPEP010000431          640 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZPEP010000431 DAZPEP010000000
VERSION     DAZPEP010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.434"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(1..607)
                     /locus_tag="ACHUT9_004675"
                     /pseudo
     CDS             complement(1..607)
                     /locus_tag="ACHUT9_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
BASE COUNT          190 a          160 c          145 g          145 t
ORIGIN      
        1 tactaccaat ccgattctgt acactatttc cgctgaaatt ataattgagc aagccggcat
       61 taataccggg atcccacaac tcaggaggaa tatatccacg cgcgcgatta ctcataaatg
      121 cctgagggat cgtcaggttg agtcgctgct gaccaacatc taaatgcgca gtagcgtcat
      181 ggatcattga ggttaatggc acgcaggcat catccaccag cagattcata ccggagacag
      241 aagccgtatt cagccccata ctggcaagct gcgcgcgtgt caggcaggga acaatccctt
      301 gttcactgtc gcccgtgtta aatgtgacat cacgcgttgc catataacca ttattcaaat
      361 agatatcgac gcgatacgtc cctggcggta attcttgccc attttcaaaa cgcgataaat
      421 cggccacagc ctagggatca tccgctaaaa agcgcggatt aaaatagagt tcggcagatg
      481 acaaaggtgc ctgtgcggca aaagcacagg cgacaaagag ccggacaaaa agaccagcca
      541 aacgatgctt acgaatatgc aagcattgtg tgtttcgctg gtaaagtctt aaattcagat
      601 atgacattat tcctccggtt taaaatcacc cggcagtcaa
//
LOCUS       DAZPEP010000432          629 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZPEP010000432 DAZPEP010000000
VERSION     DAZPEP010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.435"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            98..526
                     /locus_tag="ACHUT9_004676"
     CDS             98..526
                     /locus_tag="ACHUT9_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350929.1"
                     /translation="MINITTKDGTQVSGEVRDLVILLETDGKTVSDYLQPIPPKKDTH
                     WSMIFIPVAIYILLNLVYIIAHPLFPEKYLKTSTSIFLFISFILSAIVAYFVFHKHKS
                     ATLSICSLVFLVLIVGANVGFISYSDLTQKATQKLDSLSK"
BASE COUNT          207 a          115 c          102 g          205 t
ORIGIN      
        1 gagatacaat atttttaatt aaatcacctg attcagagga gtggtcatca caaaaagctt
       61 ccaaagacgt agagggcatc gtatttggag ggaaaaaatg attaacatta ctactaagga
      121 tggcactcaa gtcagtggtg aagtaagaga tctagtaatt cttcttgaaa ctgatggaaa
      181 aacggtgtct gattatttgc agccaatccc tcccaaaaaa gatacacact ggagcatgat
      241 tttcattccg gtagcaattt atatactact taatcttgta tacattattg ctcacccatt
      301 atttcctgaa aaatatctaa aaacatcaac atctatattt ctattcattt ccttcatatt
      361 aagtgcaata gtagcatatt ttgttttcca taagcataaa tctgccactc tatccatatg
      421 cagcttagtg ttcttagtgt taattgtagg tgctaacgta gggtttatca gttacagcga
      481 cctaacacaa aaagcgacgc aaaaattaga tagtttgtct aaataattat gttgccatgc
      541 gaagtatgca tggctgcatg tctgccttcc atttaaaatg ggctaagacc tataacccta
      601 aatattattc tttattatct tctttacca
//
LOCUS       DAZPEP010000433          626 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZPEP010000433 DAZPEP010000000
VERSION     DAZPEP010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.436"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..493
                     /gene="espX1"
                     /locus_tag="ACHUT9_004677"
     CDS             <1..493
                     /gene="espX1"
                     /locus_tag="ACHUT9_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFR1350930.1"
                     /translation="SNITIEKSNLSYGYYFCCVLTNISCFESDLSNTIFSNGEINNLF
                     IKKSNIFGTSFTNTMIKNLRCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSR
                     FEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSKMRKYLQSINECMLLIKNIV
                     HQD"
BASE COUNT          242 a          110 c           96 g          178 t
ORIGIN      
        1 atcaaatata acaatagaaa agagcaacct ttcatatggg tattattttt gttgtgtatt
       61 aaccaatata tcatgttttg aaagcgactt atcaaacacc atcttttcaa atggagaaat
      121 aaacaacctc ttcattaaaa aaagcaacat atttggaaca tcgttcacta ataccatgat
      181 aaaaaatttg cgctgtgaag atattatgcc tggccgatgg accactcaac ttgtcaataa
      241 gcacttaggt tacagataca caggagtatt caaaaccctg gcaagtattg atgacaaacc
      301 gtcccgtttc gaaattttga taccattggt acaaacttta gttcgcgata atgtaaagct
      361 aaataatgat gtgtataaag aacttaataa gtttatgcac gattacgaca aaacatcatc
      421 aaaaatgaga aaataccttc agagcatcaa cgagtgcatg ctccttataa aaaatatagt
      481 tcatcaggat taaaacactc aaagaaagag gtaattttag aatgaaatta tctgtgatga
      541 ttattaatac actgcagaga aataaaaaac aatctgaagc atgctctgaa acatcctact
      601 ctgtcactct aatgatggca ccttgc
//
LOCUS       DAZPEP010000434          619 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZPEP010000434 DAZPEP010000000
VERSION     DAZPEP010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.437"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..424
                     /locus_tag="ACHUT9_004678"
                     /pseudo
     CDS             1..424
                     /locus_tag="ACHUT9_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          212 a          107 c          119 g          181 t
ORIGIN      
        1 tgataaagaa aattcactta agcgtgtcat taatagtttt aattctgaac tcatgggata
       61 aatgtcgaat aataatataa aagttcattt aaatgaacca gagataatat ttttacatgc
      121 cgttcttcag caatacttaa gccaaagttg cggtgcgttt gtgtgcatgg cagcccagga
      181 agtgattgaa caaagggaaa gcaattctga cagcgccccc tatacgttat taaaaaacta
      241 tgctgacaga tttaaaaaat attcagcaga agagcagtac gaaattgatt tttaacatcg
      301 actggtaaac aggaattgtt atttagataa atatggcgat gcaaatatca atgattatta
      361 tagagactta gaaataaaac actcacaacg caaaaataga gcatcaggaa aaagagtaag
      421 ttaacatcat tgttaattaa ttcgaccagt cagcaaatat gctgactggt cgaacatagt
      481 ttgctaaaca gtcatcactg actgatgtca gtcacaccat ccgccagttc aatcttcggg
      541 tatttccagc gtatctcggc acgcgattgc tggtctgttg atgtagtgga ttgttgttgc
      601 tgacttttct taccctcag
//
LOCUS       DAZPEP010000435          593 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZPEP010000435 DAZPEP010000000
VERSION     DAZPEP010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.438"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..91
                     /locus_tag="ACHUT9_004679"
     CDS             <1..91
                     /locus_tag="ACHUT9_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350931.1"
                     /translation="GLENAPQTFIGLLKGKNFGKVVIRVAGDD"
     gene            289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUT9_004680"
     CDS             289..>593
                     /gene="mcbR"
                     /locus_tag="ACHUT9_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFR1350932.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAV"
BASE COUNT          172 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tga
//
LOCUS       DAZPEP010000436          591 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZPEP010000436 DAZPEP010000000
VERSION     DAZPEP010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.439"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(4..>591)
                     /locus_tag="ACHUT9_004681"
     CDS             complement(4..>591)
                     /locus_tag="ACHUT9_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HFR1350933.1"
                     /translation="LAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAAVVLPRL
                     ALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACRAFASAA
                     HLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAYYTRKMS
                     QGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS"
BASE COUNT          119 a          162 c          196 g          114 t
ORIGIN      
        1 atgttatgac ccctgcgggg tataaaaagt cccgtcgcgc atcatggcga acagaacgtc
       61 gcagcgtcgt ctcgccaggg cgataagcgc ctgattgtgt cgttttccct gactcatttt
      121 gcgggtgtag taagccctgg agagcggatc cctgagcgcg gcgaaggccg acaggaacaa
      181 cgcccgtttg agagctttat taccccgtcg cgagggatgc tcaccgcgta ttgacgagcc
      241 ggatcgccga gttaccggcg caaggccagc ataagcagcg agatgtgcgg cagaggcgaa
      301 ggcgcggcag gcgacctcgg tgaggagtct ggctgcggtc ctgacaccga ctccgggcat
      361 actggtcagg accgggtaaa gagggtgagc aagaactcgc tgttctacct caagcgccac
      421 ctcgtctctt tgcttacgca gcgtgatgag ctggagtgcc agacgtggca gtactacggc
      481 agcggcattc gtgccgggaa cgacgacggt ttgttcggcc agtgcctgag ctatgtctgc
      541 tgcaaggcgt ttacccagac gaggcgcaag tttgcagagc tgggctgcca g
//
LOCUS       DAZPEP010000437          581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZPEP010000437 DAZPEP010000000
VERSION     DAZPEP010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.440"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..581
                     /locus_tag="ACHUT9_004682"
                     /pseudo
     CDS             1..581
                     /locus_tag="ACHUT9_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          186 a          113 c          112 g          170 t
ORIGIN      
        1 aacattaatc taaataataa caacctgatc acataatcgg gtacgatttt atccgataac
       61 tataatgcta taactctggg aaatggttgg aatacaccgc tgggtgccat ctctttttac
      121 gctgcccgat cgagtagtaa gttaaataat gataccagac acgaaggtac gagctatcag
      181 gtcgcctata ataaatatct agtacaaaca gccactcgat ttagcgtggc cgcctggcgt
      241 tatgcttcgc aggattacag gacattcagc gaccatcttt acgaaaatga taaacactat
      301 catcagagtg attatgatga tttttatgat attggcagaa aaaatagcct ttctgccaat
      361 attatgcaac ctttatccaa taatctggga aatgtatcat taagtgcgct ttggcggaat
      421 tactgggggc gaagtggaaa tgctaaagat taccaattca gttactccaa taactggcaa
      481 cacattagtt atactttctc tgccagccaa tcttatgatg aaaataataa agaagaggag
      541 cgttttaatc tgtttatctc cattcctttc tactgggggg a
//
LOCUS       DAZPEP010000438          581 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZPEP010000438 DAZPEP010000000
VERSION     DAZPEP010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.441"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>581)
                     /locus_tag="ACHUT9_004683"
     CDS             complement(<1..>581)
                     /locus_tag="ACHUT9_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFR1350934.1"
                     /translation="ISGAEGWEDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCI
                     SPAKFHECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLV
                     LGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKG
                     NQGRLNKAFEEKFPLKELNNPEHDSYAISEKSH"
BASE COUNT          155 a          137 c          102 g          187 t
ORIGIN      
        1 gtgactcttt tcactgattg cgtaactgtc atgctctgga ttatttaatt ctttcagcgg
       61 aaatttttcc tcaaaggctt tattaagccg cccctggttt ccttttaccg cgaataaata
      121 atcaccgccc tgtttttgta tcttctctgc aatatctttc tggcatccca tcgcatcagt
      181 tgtgatgatt tttcctttaa tatccagcat gttaagaagt tcaggaatgg ctgtaatctc
      241 attggatttc tcatccgtct taatctgccc gagaaccaga ctgtgcattg ttgagaacgc
      301 actaatgaca tgaatcgctc ccttgcggcg actcttgtca taagagtgcc ggagcgtttt
      361 tccatcaatt gcaatgacgt ctttatcatc tgaagaatgg cagtcacgca tccagttaat
      421 aaagcactcg tgaaattttg caggactgat acaggataca actctggcaa tagtatcgtg
      481 aacaggaata ccattttcaa aatcaccata ttgcttcaaa aaatcgagat gtgtttcacc
      541 aaaatcctct atatcttccc aaccctctgc accagaaatg a
//
LOCUS       DAZPEP010000439          573 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZPEP010000439 DAZPEP010000000
VERSION     DAZPEP010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.442"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>573
                     /locus_tag="ACHUT9_004684"
     rRNA            <1..>573
                     /locus_tag="ACHUT9_004684"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          134 a          130 c          189 g          120 t
ORIGIN      
        1 gtgcaatggc ataagccagc ttgactgcga gcgtgacggc gcgagcaggt gcgaaagcag
       61 gtcatagtga tccggtggtt ctgaatggaa gggccatcgc tcaacggata aaaggtactc
      121 cggggataac aggctgatac cgcccaagag ttcatatcga cggcggtgtt tggcacctcg
      181 atgtcggctc atcacatcct ggggctgaag taggtcccaa gggtatggct gttcgccatt
      241 taaagtggta cgcgagctgg gtttagaacg tcgtgagaca gttcggtccc tatctgccgt
      301 gggcgctgga gaactgaggg gggctgctcc tagtacgaga ggaccggagt ggacgcatca
      361 ctggtgttcg ggttgtcatg ccaatggcac tgcccggtag ctaaatgcgg aagagataag
      421 tgctgaaagc atctaagcac gaaacttgcc ccgagatgag ttctccctga ccctttaagg
      481 gtcctgaagg aacgttgaag acgacgacgt tgataggccg ggtgtgtaag cgcagcgatg
      541 cgttgagcta accggtacta atgaaccgtg agg
//
LOCUS       DAZPEP010000440          561 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZPEP010000440 DAZPEP010000000
VERSION     DAZPEP010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.443"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>561)
                     /locus_tag="ACHUT9_004685"
     CDS             complement(<1..>561)
                     /locus_tag="ACHUT9_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFR1350935.1"
                     /translation="VWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGISGFI
                     GRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVRPSAD
                     LFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIREDLW
                     QDEHLLRLCLRQALQLADVVKLSEEE"
BASE COUNT          146 a          167 c          128 g          120 t
ORIGIN      
        1 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
       61 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag ctgacaaaac
      121 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
      181 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
      241 tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt tccccttgat
      301 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
      361 tatcgactcc ctcagttagc agcgttcttt gcattaacgc cccaaaagga tcatcaccga
      421 cccgacctat aaatccactt attccgccta atctggcgat tcccaccgca acgttagctg
      481 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctacgaccg
      541 catcccctaa aacccatact t
//
LOCUS       DAZPEP010000441          559 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZPEP010000441 DAZPEP010000000
VERSION     DAZPEP010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.444"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..428
                     /locus_tag="ACHUT9_004686"
     CDS             <1..428
                     /locus_tag="ACHUT9_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HFR1350936.1"
                     /translation="QLTSVARQNETCKRLLDIPGVGPLIATAAVATMGEASAFKSGRE
                     FAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKK
                     RRPASVAIVAMANKLARTVWAITAHDRKYDRNHVSIRPY"
BASE COUNT          163 a          124 c          156 g          116 t
ORIGIN      
        1 aacagcttac cagcgtggcg aggcagaatg aaacctgtaa gcggttgctg gatattcctg
       61 gcgttggacc acttattgcg acggcagcgg tggccaccat gggggaagca tcagcgttta
      121 aatcggggcg agagttcgcc gcatatgttg gtctggttcc aaaacaaact ggctccggag
      181 ggaaagtacg tctgctgggg ataagcaaac gtggtgacac ttatctcagg acattattta
      241 tccacggtgc aagagcggtg gcattagtag ctaaagagcc tggcccgtgg ataaccgaac
      301 tgaaaaaacg tcgtccagcc agtgtggcaa tcgtcgccat ggcaaacaag ctggcacgaa
      361 cagtatgggc gataaccgcc catgaccgta agtatgacag gaaccacgtc agtatcagac
      421 catattaatc gctgatacca ttaaacaatg aactcttaac aaaagggtga atgctgaaag
      481 gttgctatgg cggccagagt gatgacaaag acaggtaaga ccgtgactca ctaaacctga
      541 acagtatttt gggcttgaa
//
LOCUS       DAZPEP010000442          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZPEP010000442 DAZPEP010000000
VERSION     DAZPEP010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.445"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUT9_004687"
     CDS             <1..>557
                     /locus_tag="ACHUT9_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1350937.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLK"
BASE COUNT          115 a          160 c          157 g          125 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZPEP010000443          557 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZPEP010000443 DAZPEP010000000
VERSION     DAZPEP010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.446"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>557
                     /locus_tag="ACHUT9_004688"
     CDS             <1..>557
                     /locus_tag="ACHUT9_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1350938.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICAGRPDEGIPAVCFKLK"
BASE COUNT          111 a          162 c          159 g          125 t
ORIGIN      
        1 gacgaaaata ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccgcaac cgctgcacga tgcactggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgccgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca caaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctcggtggt cagattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtgat tgcacagtac tatgaattcc tgcgccttgg tcgtgaaggc
      421 tataccaaag tacagaatgc ttcctaccag gtcgctgcct atctggcgga tgagatcgcc
      481 aaactggggc cgtatgagtt catctgtgcc ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaaga
//
LOCUS       DAZPEP010000444          553 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZPEP010000444 DAZPEP010000000
VERSION     DAZPEP010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..553
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.447"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>553
                     /gene="feaR"
                     /locus_tag="ACHUT9_004689"
     CDS             <1..>553
                     /gene="feaR"
                     /locus_tag="ACHUT9_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFR1350939.1"
                     /translation="WQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDAS
                     RPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNN
                     PALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWI
                     AGETGMSVRSLYRMFADKGLVVAQ"
BASE COUNT          140 a          125 c          146 g          142 t
ORIGIN      
        1 tggcaggaag taaaaggcag cgacgatgcc tggttttaca ccgtttttca gcttagtggt
       61 caggcaataa tggagcagga tgagcgtcag gtgcagattg gcgctggcga tattacgtta
      121 ctcgatgcct cacgcccctg ttcgctttac tggcaggagt cttctaaaca gatttcatta
      181 cttttgccac gcactctgct ggaacaatat tttccccatc aaaaacctgt ctgcgcagaa
      241 agactggacg ctgacttacc catggtgcaa ctcagtcatc gcctgttaca ggagagcatg
      301 aataatccgg cactttctga aacagaaagt gaagctgcgc tacaggcgat ggtgtgtctg
      361 ctgcgcccgg tacttcatca gcgggaatct gttcaacctc gccgtgaacg tcagtttcaa
      421 aaagtggtta cgttgataga cgataatatt cgcgaagaga tattacgccc ggagtggata
      481 gccggagaga caggtatgtc agtacgtagt ttgtaccgaa tgtttgccga taaaggtttg
      541 gtagtcgcgc aat
//
LOCUS       DAZPEP010000445          548 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZPEP010000445 DAZPEP010000000
VERSION     DAZPEP010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.448"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..516
                     /locus_tag="ACHUT9_004690"
     CDS             <1..516
                     /locus_tag="ACHUT9_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSso6 family transposase"
                     /protein_id="HFR1350940.1"
                     /translation="LESALKEAVTEWRLYPVVEALQAMRGIQFITAVGLISELGDLTR
                     FEHPRQLMSWFGITPSEYSSGGSRHQGSITKAGNSYARKLLVEAAWSYRHPARISPAI
                     QKRQENLPRPVIDRAWDAQLRLCKRYRKLQAKGKNVNITIVAVARELAGFIWDMGRIA
                     MSVAQQPQCHK"
BASE COUNT          159 a          122 c          144 g          123 t
ORIGIN      
        1 ctggaatccg ccctgaagga ggccgttacc gaatggaggc tgtatccggt agtagaggcg
       61 ctgcaagcca tgcgagggat tcagtttatt actgcggttg gattaatttc tgaactgggt
      121 gatctgaccc gttttgaaca tccacgacaa ctgatgtcat ggtttggtat cacgccatca
      181 gaatattcat cgggtggaag cagacatcag ggcagcatta ccaaagccgg aaacagctat
      241 gccagaaagc tattggtcga ggcagcgtgg agttaccgtc atccggcacg catcagtccg
      301 gcaatacaaa aaaggcagga aaatttaccc cgccccgtca ttgacagagc atgggatgct
      361 caactcaggc tttgtaagag gtatcgaaaa cttcaggcca aaggaaagaa tgtcaatatt
      421 acaattgttg ctgttgcacg tgagctggcg ggttttatct gggatatggg ccgaatagca
      481 atgtctgtcg cacaacaacc acaatgtcac aaataaaaaa tgagaataac cagttccctg
      541 aaggcgac
//
LOCUS       DAZPEP010000446          546 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZPEP010000446 DAZPEP010000000
VERSION     DAZPEP010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.449"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..546
                     /locus_tag="ACHUT9_004691"
                     /pseudo
     CDS             1..546
                     /locus_tag="ACHUT9_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAZPEP010000447          531 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZPEP010000447 DAZPEP010000000
VERSION     DAZPEP010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..531
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.450"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
BASE COUNT          159 a          127 c          105 g          140 t
ORIGIN      
        1 gacagacctg tgctatcgga aagaaaaaag gccatcagca aaactctgat ggcctgaatc
       61 acctttacca atattgtatg aaaaaacacg cacgactaat tgacaataat tttcatttcc
      121 attaaaaaag atcgcgtagc attcttaatt catgaggaac tttacccgcc agcaatctga
      181 gtagcgaaag ctgttcatcc ccaatgtttt tgctggcggg tccttttttc tacggtcctc
      241 ctccccggag gggggcattt cattattttt ctaatcgtta ctggtgagaa ggcggaagaa
      301 aaagccagtt cttcgcccct aaataacagc atgattccag tccatgcaaa atctgatttc
      361 cagctcacac ataccgagca ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt
      421 tcccggggtt tgcttagaat attttgtaag ttgtccgaag agatatttac aacaccagaa
      481 tgatgcatta tcagtcccct gccagaggtt cactacgtaa cctgaccgac a
//
LOCUS       DAZPEP010000448          529 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZPEP010000448 DAZPEP010000000
VERSION     DAZPEP010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.451"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>529
                     /locus_tag="ACHUT9_004692"
     CDS             <1..>529
                     /locus_tag="ACHUT9_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1350941.1"
                     /translation="GGFVLHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTID
                     RWGYGVTSALSPYHENRIALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSA
                     IMNITRSDGKNIPFAADIYDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVS
                     CLAHYQQSSEAEKIAQ"
BASE COUNT          154 a          102 c          134 g          139 t
ORIGIN      
        1 cggtggtttt gtattgcata gcggcggcct gaccttcagt aatgacagtt ttagcgactc
       61 cgatacactg gcggtagttc aggctccagg tgctcaagga gcgcgaataa attatggcaa
      121 cagtactatc gatcgatggg gttatggtgt caccagcgct ctttctcctt atcatgaaaa
      181 ccgtatcgcg ctggatatca acgatcttga gaacgatgtt gaattaaaaa gtaccagtgc
      241 agtagctgta ccgcgtcagg gttcagtcgt ctttgctgat tttgaaaccg tgcaagggca
      301 atcagccatt atgaacatca cacgaagtga tggtaaaaat attccatttg ctgcagatat
      361 ttatgatgag caaggcaatg tcattggtaa tgttggacag ggtggacaag catttgttcg
      421 tggcattgag cagcagggaa atatcagcat taaatggctc gaagaaagta aacccgtaag
      481 ttgtcttgcg cattatcaac aaagttcaga agcagaaaaa atagcgcaa
//
LOCUS       DAZPEP010000449          522 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZPEP010000449 DAZPEP010000000
VERSION     DAZPEP010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.452"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>522)
                     /locus_tag="ACHUT9_004693"
     CDS             complement(<1..>522)
                     /locus_tag="ACHUT9_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350942.1"
                     /translation="FLGVTTWIFMAAGGAESVAVYVNDVKGGSKSFVKVIILAGIFIG
                     VLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAYFGLPEALMNRFVGLVSFTAMFGS
                     LLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPARAAWIQFLIVIPLMIIPMLGSNT
                     VQDLMNTIINMTA"
BASE COUNT          143 a          141 c          133 g          105 t
ORIGIN      
        1 cggcggtcat attaataata gtattcatca gatcctgcac ggtattggaa ccgagcatcg
       61 ggataatcat cagcgggatg acgatcagga actggatcca cgctgcgcgc gccggaacgc
      121 cgttttcgtt cagttcgacg gttttcttac caaagatgcc ttccgggatt tcggagaaga
      181 aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacatcgcg gtaaaggaca
      241 ccagaccgac aaagcgattc atcaacgctt ccggtagacc aaaatacgcc gccatgccgt
      301 ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg aagacgttaa
      361 tcagcaccga ggagacggaa tacagtacgc cgataaaaat tccggcgagg atgatcactt
      421 taacgaacga tttcgaaccg cctttgacgt cgttaacgta cacagcgacg gactccgcgc
      481 cacctgcggc cataaagatc caggtggtaa cgccgaggaa cg
//
LOCUS       DAZPEP010000450          511 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZPEP010000450 DAZPEP010000000
VERSION     DAZPEP010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.453"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>511
                     /locus_tag="ACHUT9_004694"
     CDS             <1..>511
                     /locus_tag="ACHUT9_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350943.1"
                     /translation="EGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSE
                     QGLATRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYEL
                     ATAWNTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTT
                     AANLDIDIP"
BASE COUNT          109 a          147 c          187 g           68 t
ORIGIN      
        1 gtgaaggcca ccgtgtggcg gtccactacg gctatgacga taaaggccgt ctgacgggcg
       61 aacggcagac ggtggagacc ccggagacgg gggagatgct gtgggagcat gagacggggc
      121 acgcgtacag cgaacagggg ctggcgaccc gtcaggagcc ggacggcctg ccgccggtgg
      181 agtggctgac gtatggcagc ggttatcttg cggggatgaa gctgggcgga acaccgctgg
      241 tcgagtacac gcgggaccgg ctgcaccgtg agacggcccg cagcttcggc ggggaggcat
      301 atgaacttgc caccgcctgg aataccagcg gccagctccg gagcaggcac ctgaaccttc
      361 cgcagcttga ccgtgactac gactggaacg acaacggaca gctaatccgc atcagtggcc
      421 cgcaggagag ccgggagtac cgttacagcg acacggggag gctgacgggc gtccacacca
      481 cggcagcgaa cctggatatc gatatcccgt a
//
LOCUS       DAZPEP010000451          494 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZPEP010000451 DAZPEP010000000
VERSION     DAZPEP010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.454"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>494
                     /gene="fimZ"
                     /locus_tag="ACHUT9_004695"
     CDS             <1..>494
                     /gene="fimZ"
                     /locus_tag="ACHUT9_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFR1350944.1"
                     /translation="MSIEVLLQKNSELQIVLKTDDYRITIDYLRTRPVDLIIMDIDLP
                     GTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQ
                     MILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGLSNKEIADKLLL
                     SNKTV"
BASE COUNT          160 a           92 c           93 g          149 t
ORIGIN      
        1 atgtctattg aagttctgtt gcaaaaaaac agtgaattgc agattgtcct gaaaacggat
       61 gattaccgca taaccatcga ttatctccga acccgtcctg ttgatttaat cattatggat
      121 atagacttgc ccggaacaga cggttttacc ttcctgaaaa ggatcaaaca aatccagagc
      181 acagtgaaag tgttattttt atcatcgaaa tcagaatgct tttatgctgg cagagcgata
      241 caagctggtg ctaacggttt tgtcagtaaa tgcaatgatc agaatgatat ttttcatccc
      301 gttcagatga tcctctccgg atacacgttt tttcctagcg aaacgctcaa ctatataaaa
      361 agcaataaat gtagtacgaa tagttcaacg gtcactgtgc tatctaatcg tgaagtgacc
      421 atattacgtt atctggttag cggattatct aataaagaaa ttgccgataa gttattactt
      481 agcaataaaa cagt
//
LOCUS       DAZPEP010000452          489 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZPEP010000452 DAZPEP010000000
VERSION     DAZPEP010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.455"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUT9_004696"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="ACHUT9_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HFR1350945.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAZPEP010000453          472 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZPEP010000453 DAZPEP010000000
VERSION     DAZPEP010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.456"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            30..206
                     /locus_tag="ACHUT9_004697"
     CDS             30..206
                     /locus_tag="ACHUT9_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350946.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          168 a           55 c          109 g          140 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcaggcca
      421 gggaaagatc atcagaagtt cactttttgt actaaataat tcgcatttta tg
//
LOCUS       DAZPEP010000454          469 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZPEP010000454 DAZPEP010000000
VERSION     DAZPEP010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.457"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>469
                     /locus_tag="ACHUT9_004698"
     rRNA            <1..>469
                     /locus_tag="ACHUT9_004698"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          129 a           99 c          145 g           96 t
ORIGIN      
        1 tcctgactga ccgatagtga accagtaccg tgagggaaag gcgaaaagaa ccccggcgag
       61 gggagtgaaa aagaacctga aaccgtgtac gtacaagcag tgggagcacg cttaggcgtg
      121 tgactgcgta ccttttgtat aatgggtcag cgacttatat tctgtagcaa ggttaaccga
      181 ataggggagc cgaagggaaa ccgagtctta actgggcgtt aagttgcagg gtatagaccc
      241 gaaacccggt gatctagcca tgggcaggtt gaaggttggg taacactaac tggaggaccg
      301 aaccgactaa tgttgaaaaa ttagcggatg acttgtggct gggggtgaaa ggccaatcaa
      361 accgggagat agctggttct ccccgaaagc tatttaggta gcgcctcgtg aattcatctc
      421 cgggggtaga gcactgtttc ggcaaggggg tcatcccgac ttaccaacc
//
LOCUS       DAZPEP010000455          468 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZPEP010000455 DAZPEP010000000
VERSION     DAZPEP010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.458"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            85..444
                     /locus_tag="ACHUT9_004699"
                     /pseudo
     CDS             85..444
                     /locus_tag="ACHUT9_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
BASE COUNT          114 a          109 c          137 g          108 t
ORIGIN      
        1 gtggtgatgt ctatctcaca ccgatgaaca tgaccacgaa accctccgat ggcagtaagg
       61 ccggtaagca gaaggataac gccaatgcag acgaaacaac gtcttgatgt accgctgagt
      121 ctgaaatctg tcagtgactc cggtgagttt gaagggtatg gctccgtctt tggtgtaaag
      181 gacagccacg atgatgtggt gatgtccggg gcatttgctg cttccctgcg ggcgtggagt
      241 gacagaaaag cgttacctgc gctgctctgg cagcaccgca tggatgaacc catcggtgtt
      301 tacaccgaaa tgaaggaaga cgatgtcggg ctttacgtca ggggacggtt gcttattgat
      361 gatgatcccc tcgcaaaacg tgcacatgca cacatgaagg ccggttcgtt aaccggcctt
      421 tctattgggt acgtcctgaa agactgaggt agcctgagtt taacggac
//
LOCUS       DAZPEP010000456          467 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZPEP010000456 DAZPEP010000000
VERSION     DAZPEP010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.459"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>467
                     /locus_tag="ACHUT9_004700"
     CDS             <1..>467
                     /locus_tag="ACHUT9_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1350947.1"
                     /translation="VKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTG
                     GFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTA
                     VRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALG"
BASE COUNT          119 a          108 c          152 g           88 t
ORIGIN      
        1 gtgaaagaaa gtaatcagcg atggtgctct gacgggttcg agttccgctg tgataacgga
       61 gaaaaactgc gagtcacgtt cgcgctggac tgctgtgacc gtgaggcact gcactgggcg
      121 gtcactacgg gcggcttcaa cagtgaaaca gtacaggacg tcatgctggg agcggtggaa
      181 cgccgcttcg gcaacgagct tccggcgtct ccagtagagt ggctgacgga taatggttca
      241 tgctaccggg ctaatgaaac acggcagttt gcccggatgt tggggcttga accgaagagc
      301 acggcggtgc ggagtccgga gagtaacggc atagcagaga gcttcgtgaa aacgataaag
      361 cgtgactaca tcagtgtcat gcccaaacca gacgggttaa cggcagcaaa gaaccttgca
      421 gaggcgttcg agcattataa cgaatggcat ccgcatagtg cactggg
//
LOCUS       DAZPEP010000457          457 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZPEP010000457 DAZPEP010000000
VERSION     DAZPEP010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.460"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..118
                     /locus_tag="ACHUT9_004701"
     CDS             <1..118
                     /locus_tag="ACHUT9_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFR1350948.1"
                     /translation="KLTKSECEKMLSGEKEAGASREQKVKLINFNCEKLQSS"
     gene            225..>457
                     /locus_tag="ACHUT9_004702"
     CDS             225..>457
                     /locus_tag="ACHUT9_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFR1350949.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPL"
BASE COUNT          161 a           93 c           99 g          104 t
ORIGIN      
        1 aaaactaacg aaatcagaat gcgaaaaaat gctctcaggg gaaaaagaag caggcgcttc
       61 cagagagcag aaagtaaaac tgataaattt taattgcgag aaacttcagt cctcgtgaat
      121 ttcattatat ccagattaga acttcatagc tgattattaa aaatcaacca cacccgccag
      181 tattctgtat atttactggc ggtcatatcg tgagaggtat ggcaatgaat cttgtgacac
      241 tcaaaacgtg gggaaaactc agatatccgg ataacccacc atcaatatca acgctgagac
      301 gatgggcaag gaatggaaac atttatcctg cacctgaact acacgggagg agttacaggg
      361 tggttccgga ggctttctat atcaacccaa ataaggttga taccgatata acacaccatc
      421 agcctaatgg gcgacaaggg agagacagtc cgttaat
//
LOCUS       DAZPEP010000458          456 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZPEP010000458 DAZPEP010000000
VERSION     DAZPEP010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.461"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            2..388
                     /locus_tag="ACHUT9_004703"
     CDS             2..388
                     /locus_tag="ACHUT9_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFR1350950.1"
                     /translation="MKGQVVELVRLSGRETLRQLEAKTGATRYLMSVLARELVASGDV
                     YNSGYGLFPSEQARKDWQNARKKLSRANLKKPSVVDPDLIWSLPDGEIRRYDRRQNII
                     CSECRKSEAMQRVLAFYQGNFQKVLL"
BASE COUNT          135 a           95 c          122 g          104 t
ORIGIN      
        1 aattaaaggg caggttgttg aacttgtacg tctgagcggt cgcgagacgt tgcggcaact
       61 ggaagccaag acaggtgcga caagatatct gatgagtgtt ctcgccagag agctggttgc
      121 cagtggcgat gtatacaact ctggttacgg gttattcccg tctgaacagg cgcgtaagga
      181 ctggcaaaac gcccgcaaaa aactctcaag ggcaaatctg aagaaaccat ctgtggttga
      241 tcctgacctt atctggtcat taccagacgg agaaatacgc cgctacgaca ggcgtcaaaa
      301 cataatctgt agcgagtgcc ggaagagcga agctatgcag cgtgtactgg ctttctatca
      361 gggtaatttt cagaaggtgc tgttgtgagc caaattaaca atcggaactt cgtgaagaga
      421 aagcataatc caaaatctga ataattaagt tcagca
//
LOCUS       DAZPEP010000459          448 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZPEP010000459 DAZPEP010000000
VERSION     DAZPEP010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.462"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..339
                     /locus_tag="ACHUT9_004704"
     CDS             <1..339
                     /locus_tag="ACHUT9_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFR1350951.1"
                     /translation="NYREKITLEQLSKKFRASVSYICHEFTKEYRISPINYVIQRRMT
                     EAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQFKNCFAEQEILS
                     EFPQPVSLAG"
     gene            complement(382..>448)
                     /locus_tag="ACHUT9_004705"
     CDS             complement(382..>448)
                     /locus_tag="ACHUT9_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350952.1"
                     /translation="GTDEIMIYVAGRQILKDYQNK"
BASE COUNT          125 a           95 c           90 g          138 t
ORIGIN      
        1 aattatcgcg aaaaaatcac tttagaacag ttatcgaaaa aatttcgtgc cagcgtcagt
       61 tatatttgcc atgaatttac caaagagtat cgtatttccc ctattaacta tgttattcaa
      121 cggcgtatga cggaagcaaa atggtcactc actaatactg aattatcaca ggcagagatc
      181 tcctggcgtg tgggttatga aaatgtcgat cactttgcca aactgttttt gcgccatgtc
      241 ggttgttcgc ccagcgatta ccgtaggcaa tttaaaaatt gttttgcgga acaagaaatc
      301 ttatctgaat ttcctcaacc ggtaagtctt gccggataaa tctggcggcg cactttgtta
      361 aaaagctgcg ccgcctgcag attatttgtt ctgatagtct ttcaggatct gccgacctgc
      421 tacgtaaatc ataatttcgt ctgtaccg
//
LOCUS       DAZPEP010000460          442 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZPEP010000460 DAZPEP010000000
VERSION     DAZPEP010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.463"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>442
                     /locus_tag="ACHUT9_004706"
     CDS             <1..>442
                     /locus_tag="ACHUT9_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1350953.1"
                     /translation="HRLVHYTRTQYAEPLVESRYLYDPLGRRVAKRVWRRERDLTGWM
                     SLSRKPQVTWYGWDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGELAKTQRRSL
                     ADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL"
BASE COUNT           94 a          127 c          153 g           68 t
ORIGIN      
        1 caccggctgg tgcactacac gcggacacaa tatgcagagc cgctggtcga aagtcgctat
       61 ctttacgacc cgctgggccg cagggtggca aaacgggtat ggcggcgtga acgggacctg
      121 acgggctgga tgtcgctgtc acggaaaccg caagtgacct ggtacggctg ggacggcgac
      181 cggctgacca caatacagaa cgacagaacc cgcatccaga cgatttatca gccggggagc
      241 ttcacgccac tcatcagggt cgaaactgcc accggtgagc tggcgaaaac gcagcgccgc
      301 agcctggcgg atgcccttca gcagtccggc ggcgaagacg gtggcagtgt ggtgttcccg
      361 ccggtgctgg tgcagatgct cgaccggctg gaaagtgaaa tcctggctga ccgggtgagt
      421 gaggaaagcc gccgctggct gg
//
LOCUS       DAZPEP010000461          436 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZPEP010000461 DAZPEP010000000
VERSION     DAZPEP010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.464"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>436
                     /locus_tag="ACHUT9_004707"
     CDS             <1..>436
                     /locus_tag="ACHUT9_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFR1350954.1"
                     /translation="WHIAANIQFPPNFDESKQYPAIISVHPFGSCKEQTSGNIYGKAL
                     AEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLPYVNAERIGVLGV
                     CGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
BASE COUNT          108 a           84 c          123 g          121 t
ORIGIN      
        1 tggcatattg ctgccaatat tcagtttcca ccaaattttg atgagtcaaa acagtatccg
       61 gcgattatca gcgtccatcc ttttggcagt tgtaaggagc agacgtctgg caatatctat
      121 ggcaaggcat tggctgaaaa aggctatctc gttctcgctt atgacgccag tttccaggga
      181 gaatctggtg gggagcctcg ctggatagaa gatccgacac agcgcgtaga ggatattagc
      241 cgtgttatcg attatgcagt aactctgcca tatgttaatg ctgaacgtat tggcgtgctg
      301 ggagtgtgtg ggggtggtgg ctatgccatt aatgccgcgc tgacagaaaa acgaattaaa
      361 gcagtagtga gtattacggg tgttaatatc gggcgtcttt ttcgggaagg tttcagcaat
      421 tacgatccga ttggcg
//
LOCUS       DAZPEP010000462          432 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZPEP010000462 DAZPEP010000000
VERSION     DAZPEP010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.465"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..166
                     /locus_tag="ACHUT9_004708"
     CDS             <1..166
                     /locus_tag="ACHUT9_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350955.1"
                     /translation="SEEFLFDNSLEKDENSSDNCFEVEKYNAYEAYNSENLYFTREEY
                     TYDYDLLNAI"
BASE COUNT          147 a           91 c           78 g          116 t
ORIGIN      
        1 ctcagaggaa ttcctttttg ataatagcct cgagaaagac gaaaattctt cggacaattg
       61 ctttgaggtc gaaaaatata acgcatatga agcatataac tcagagaatc tatattttac
      121 cagagaggaa tatacgtatg attacgacct tttaaatgca atatagacat aataacttct
      181 tacaaaatct ataattaata ccgtagatat tcattcatct taccctgtgc accaaaagca
      241 aaaactcact gacgcgggtt tgaaactgat ggcaaccgca aaaatgcctg atgcgctacg
      301 cttatcaggc ctacgccatc tctacaatat attgaatttg cgtgcttttg taggcaggat
      361 aaggcgttca cgcagcatcc ggcatgaata aagcgcactc aacaatctga aacccgccgg
      421 aacggtttat ta
//
LOCUS       DAZPEP010000463          429 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZPEP010000463 DAZPEP010000000
VERSION     DAZPEP010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.466"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>429
                     /locus_tag="ACHUT9_004709"
     CDS             <1..>429
                     /locus_tag="ACHUT9_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFR1350956.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLST"
BASE COUNT          123 a           92 c          103 g          111 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcac
//
LOCUS       DAZPEP010000464          420 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZPEP010000464 DAZPEP010000000
VERSION     DAZPEP010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.467"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            41..116
                     /locus_tag="ACHUT9_004710"
     tRNA            41..116
                     /locus_tag="ACHUT9_004710"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:74..76,aa:Ile,seq:cat)
BASE COUNT          117 a           79 c           98 g          126 t
ORIGIN      
        1 tttattgcgt ggtttataag tattgttatt gcacactcaa ggccctttag ctcagtggtt
       61 agagcaggcg actcataatc gcttggtcgc tggttcaagt ccagcaaggg ccaccaaatc
      121 attatttacg tatcaggaag agttaagggt acaaggggta ctcgatggcg gtctatattt
      181 catgactaac gccatttcaa ggaagaagtg cggtgggtgg tttcgaatga gggctggtgt
      241 tttggtacgt ggcacgccac tgcaatgtat ttatccatca gtactcattc aaaagtacct
      301 ggaaaaaatg cctgctatgc caagcgggca aattgcttac atgtggaatt aagtaattaa
      361 cgataaagcc agagtattat catttattat accccttcat ttaatcaggt taattactca
//
LOCUS       DAZPEP010000465          408 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZPEP010000465 DAZPEP010000000
VERSION     DAZPEP010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.468"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            54..130
                     /locus_tag="ACHUT9_004711"
     tRNA            54..130
                     /locus_tag="ACHUT9_004711"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="ACHUT9_004712"
     tRNA            173..248
                     /locus_tag="ACHUT9_004712"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
BASE COUNT          115 a           86 c           97 g          110 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcg
//
LOCUS       DAZPEP010000466          400 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZPEP010000466 DAZPEP010000000
VERSION     DAZPEP010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.469"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
BASE COUNT          111 a           95 c           68 g          126 t
ORIGIN      
        1 ttccgcagca gggatgaaac attcaagcat ccagtatcag tagatattgc attagtgtaa
       61 ctttaaaaca agtaataatt tattatccaa taataacact gtgttccttc cccaggtgat
      121 taccctgggg ttattttctg aaagtttcct ttgcaaaagg atagcgttgt aaattacttt
      181 aacgaattac tccatgaact cattaaagtt ctttcgtaat actttatcgt caaacacgtt
      241 atagctggca tcaaaaacca cagatttacc ctgccagcgg gtaatcccct cctctggcgt
      301 tttcggttga actggtatcc ccgtggattt gcccctatat ttccagacat ctgttatcac
      361 ttaacccatt acaagcccgc tgccgcagat attcccgtgg
//
LOCUS       DAZPEP010000467          397 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZPEP010000467 DAZPEP010000000
VERSION     DAZPEP010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.470"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>397)
                     /locus_tag="ACHUT9_004713"
     CDS             complement(<1..>397)
                     /locus_tag="ACHUT9_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1350957.1"
                     /translation="DVTWLFSEDGKNWYEEQKNFASDTIKMVYTGDGRVVWVGKDVTG
                     IEPRNASVIEVPDITANRRITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVSARTGE
                     WEEDLLLGLISDEDREKLKAYRIYAKSLQA"
BASE COUNT           86 a          115 c           89 g          107 t
ORIGIN      
        1 gcctgcagcg atttcgcgta aatacggtac gctttcagtt tttcccggtc ttcgtcgctg
       61 attagcccca gcagcaggtc ttcttcccat tcccctgtcc gggcactgac ctgagccaga
      121 agggcatcac gctcatcttc cgctttgagt ctgtagtcaa agacaaattc atcattgcgg
      181 taaaaccagt aacccggcgc ggtaatgcgg cggttggcgg taatatcagg aacttcaata
      241 acactggcgt tacggggttc aatgcctgtc acatccttac cgacccacac cacgcgcccg
      301 tctccggtgt aaaccatttt tatggtgtca ctggcaaagt tcttctgttc ttcataccag
      361 tttttgccgt cttccgaaaa aagccaggtg acatcat
//
LOCUS       DAZPEP010000468          395 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZPEP010000468 DAZPEP010000000
VERSION     DAZPEP010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.471"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>395
                     /locus_tag="ACHUT9_004714"
     rRNA            <1..>395
                     /locus_tag="ACHUT9_004714"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          106 a           93 c          119 g           77 t
ORIGIN      
        1 cgggaactca aaggagactg ccagtgataa actggaggaa ggtggggatg acgtcaagtc
       61 atcatggccc ttacgaccag ggctacacac gtgctacaat ggcgcataca aagagaagcg
      121 acctcgcgag agcaagcgga cctcataaag tgcgtcgtag tccggattgg agtctgcaac
      181 tcgactccat gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg gtgaatacgt
      241 tcccgggcct tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa agaagtaggt
      301 agcttaacct tcgggagggc gcttaccact ttgtgattca tgactggggt gaagtcgtaa
      361 caaggtaacc gtaggggaac ctgcggttgg atcac
//
LOCUS       DAZPEP010000469          394 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZPEP010000469 DAZPEP010000000
VERSION     DAZPEP010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.472"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..63
                     /locus_tag="ACHUT9_004715"
     CDS             <1..63
                     /locus_tag="ACHUT9_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350958.1"
                     /translation="GKKLVAGMDVNIDDEIGDDE"
     gene            63..>394
                     /gene="yhaV"
                     /locus_tag="ACHUT9_004716"
     CDS             63..>394
                     /gene="yhaV"
                     /locus_tag="ACHUT9_004716"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFR1350959.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          119 a           72 c          101 g          102 t
ORIGIN      
        1 ggaaagaaac ttgtcgctgg catggacgtc aacattgatg atgagattgg cgacgacgaa
       61 taatggattt tccacaaagg gttaatggtt gggcgctata tgctcatccc tgttttcagg
      121 aaacctacga cgctttagtt gccgaagtcg agacattaaa gggaaaagat cctgaaaatt
      181 atcagagaaa agccgccaca aagttattgg cggtagtcca taaagtgatt gaggagcata
      241 tcacggtcaa tccatcatca ccggcattcc gtcatggcaa gtcgttaggc tctgggaaaa
      301 ataaagactg gtcacgggta aaatttggtg caggtcgtta tcgtctcttc tttcgttata
      361 gtgaaaaaga gaaagtcatc attctgggat ggat
//
LOCUS       DAZPEP010000470          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZPEP010000470 DAZPEP010000000
VERSION     DAZPEP010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.473"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>389
                     /locus_tag="ACHUT9_004717"
     CDS             <1..>389
                     /locus_tag="ACHUT9_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HFR1350960.1"
                     /translation="HDNETELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVAS
                     DVNDPATTCTCALYGFQFQGAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQS
                     KNSQTSSLWDAFAYTLAILYYENFKNA"
BASE COUNT          110 a           80 c           94 g          105 t
ORIGIN      
        1 ttcatgacaa tgaaaccgag ttgatttacg tcaagaaagg ggtagcaagg tttaccatcg
       61 attcttcgtt atatgtcgcg cacgccgatg acattgtggt gatagaacgt ggcaggctgc
      121 atgcggtggc ctctgacgtt aacgatccag caacgacgtg tacctgtgcg ctgtacggct
      181 ttcagtttca gggggctgag gaaaatcagc tactgcaacc gcattcttgc ccggtaattg
      241 ccgcagggca gggaaaagaa gtcattaaaa ccttatttaa tgagctaagt gtgattttgc
      301 cgcaaagtaa aaatagccaa acatcttcgt tatgggatgc atttgcctat acattagcaa
      361 ttctttacta cgaaaacttt aaaaatgct
//
LOCUS       DAZPEP010000471          389 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZPEP010000471 DAZPEP010000000
VERSION     DAZPEP010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.474"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>389)
                     /locus_tag="ACHUT9_004718"
     CDS             complement(<1..>389)
                     /locus_tag="ACHUT9_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFR1350961.1"
                     /translation="KMPADIRLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLW
                     LVRGGVAKLDEGHRLAALWQALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEV
                     LPAPLPPYRVLTGLVDRFGRTQTFHRE"
BASE COUNT           71 a          134 c          112 g           72 t
ORIGIN      
        1 ttcgcggtgg aacgtctgtg tgcgcccgaa gcggtccacc agcccggtca gtacccggta
       61 cggcggcagc ggcgcaggca gcacctcatc cgcttccggc acccgctcac accagccgag
      121 cagccaccac ggcccctgcg gactgtttgt cgccagataa cgatgcggac ttaagcggag
      181 ttcttccggc agcgcctgcc agagtgcggc cagccggtga ccttcatcca gtttcgccac
      241 gccgccgcgc accagccaga gtgattcgct gcggctgtaa ccgtcctcac cgggaaacag
      301 gtgctcaaaa tacaggcttc tgccgccgtt atcactgagt atcagtgtgt tatcgcgcag
      361 ctgtaagcgg atatccgcag gcattttcc
//
LOCUS       DAZPEP010000472          385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZPEP010000472 DAZPEP010000000
VERSION     DAZPEP010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.475"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>385
                     /locus_tag="ACHUT9_004719"
     CDS             <1..>385
                     /locus_tag="ACHUT9_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350962.1"
                     /translation="RVTEQVNPEGLDYRFEYGESRVIITDSLNRREVLYTEGEGGLKR
                     VVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGKLTSVILPDGRTVRYG
                     YNSQRQVTSVTYPDGLRSSREYDEKG"
BASE COUNT           87 a           87 c          158 g           53 t
ORIGIN      
        1 gtcgggtgac ggagcaggtc aacccggagg ggctggacta ccgctttgag tacggagaga
       61 gccgggtaat catcacggac agcctgaacc ggcgggaggt gctgtacacg gaaggcgagg
      121 gtggcctgaa gcgggtggtg aagaaggagc atgccgacgg gagcatcacc cgcagcgagt
      181 atgatgaggc ggggaggctg aaggcgcaga cggatgcggc gggacggcgg acggagtacc
      241 gactgcatat ggcgtctgga aagctgactt cagtgatact gccggacggc aggacggtac
      301 ggtatggcta taacagccag cggcaggtga cgtcggtgac gtacccggac gggcttcgca
      361 gcagccggga gtatgatgag aaggg
//
LOCUS       DAZPEP010000473          385 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZPEP010000473 DAZPEP010000000
VERSION     DAZPEP010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.476"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(218..>385)
                     /locus_tag="ACHUT9_004720"
     CDS             complement(218..>385)
                     /locus_tag="ACHUT9_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFR1350963.1"
                     /translation="LHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
BASE COUNT          128 a           66 c           71 g          120 t
ORIGIN      
        1 tatctgaatc aacttttcct ttgctaaagg tatcgaaata actgcaaggc tgtattttgt
       61 aattgccaat ttaaaaatgt caatttgttt ttaattatgt ttttgtaata agtttgaaga
      121 cgcaaaaaaa taatataata acaaaaatcc tccagccttg taggccagag gataaaatac
      181 ttaattgagg aaaatatcag ggaaaagtat ttctgactta actttcccgg ctggagagtg
      241 aatcaatgta atcagcgtac cattgcatca tttcacggcg gccatcgaga tacagggcat
      301 gattataggt acccctgatg gaatttttat ccacatgagc taactgcatt tcaatccagg
      361 cggaattaaa gccttgttca tgcag
//
LOCUS       DAZPEP010000474          383 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZPEP010000474 DAZPEP010000000
VERSION     DAZPEP010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.477"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..354
                     /locus_tag="ACHUT9_004721"
     CDS             <1..354
                     /locus_tag="ACHUT9_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFR1350964.1"
                     /translation="LDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQ
                     RNGGVQDVRYGYEMYYTPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRP
                     LQKLINGTWYNVASI"
BASE COUNT          105 a           82 c          100 g           96 t
ORIGIN      
        1 ctggatgttt atgccaatag cgtgcatgtg ttgcgttttc agagtggcag tatccagagt
       61 aataaagctg taaacgttac aggacgggta acaccgtcag actacggaaa ctttgatgcc
      121 cgttaccagc agcgaaatgg tggcgtgcag gatgtgcggt atggttatga aatgtattac
      181 acccccggca gtaacaccgt ttcgtggaca tttcgctcac cttcgggaca cgggctgtca
      241 gggatatcga tatcggatac cggccgtaac tcagcggata acgtcaacgg cgtgtattac
      301 cgaccgctgc aaaaactgat taatggcacc tggtataacg tagcgagtat ttaacaatgt
      361 tgcatttaaa aaatattacg gca
//
LOCUS       DAZPEP010000475          380 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZPEP010000475 DAZPEP010000000
VERSION     DAZPEP010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.478"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            50..373
                     /locus_tag="ACHUT9_004722"
     CDS             50..373
                     /locus_tag="ACHUT9_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFR1350965.1"
                     /translation="MKWVVIDTVIQPTCGISFSAIWGDMKMIIWYQSTIFLPPGSIFT
                     PVKLGIILKEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT          138 a           63 c           65 g          114 t
ORIGIN      
        1 tgaacatcca aatgaactat attaaatacc gtgggataag acataacaaa tgaagtgggt
       61 agtaattgac acggtaattc aacctacatg cggcatatct ttttcagcca tatggggtga
      121 tatgaaaatg atcatctggt atcaatctac tatatttctt cctcctggaa gtatatttac
      181 accggttaag cttggtatta tccttaagga aaaagaatat cctattacta tttataacat
      241 tgcaccattc aacaagaatt tatggagttt actcaaaagc agtcaagagt gtcctccagg
      301 agaaagcaaa ataacaaata aatgtttaca taatagttgc attataaaaa tatgcccata
      361 tgggctcaag taatgggctt
//
LOCUS       DAZPEP010000476          367 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZPEP010000476 DAZPEP010000000
VERSION     DAZPEP010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.479"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>367
                     /locus_tag="ACHUT9_004723"
     CDS             <1..>367
                     /locus_tag="ACHUT9_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFR1350966.1"
                     /translation="SRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQ
                     IRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYSALRPH
                     EYNGGLPPNESENRYWKNSN"
BASE COUNT          106 a           85 c          107 g           69 t
ORIGIN      
        1 agcaggctca ccatgaaagc gctggaaatg gcatgggaaa cccgtggtaa gcccggcggg
       61 gtgatgttcc acagcgatca gggcagtcat tatacgagca ggcagttccg gcagttattg
      121 tggcgatacc agatcagaca gagtatgagc cggcgcggaa actgctggga taacagccca
      181 atggaacgct tcttcaggag tctgaagaac gaatggatgc cggtggtggg ttacgtaagc
      241 ttcagcgagg cagctcacgc cataacggac tatatcgttg gatattacag cgcactaaga
      301 ccgcacgaat ataacggtgg gttaccccca aacgaatcgg aaaatcgata ctggaaaaac
      361 tctaact
//
LOCUS       DAZPEP010000477          366 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZPEP010000477 DAZPEP010000000
VERSION     DAZPEP010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.480"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            47..>366
                     /locus_tag="ACHUT9_004724"
     CDS             47..>366
                     /locus_tag="ACHUT9_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFR1350967.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQVSIVYEAGPCGYGLYRRLVKSGFDCMVCAPSLIPKKPGERVKTDRRD
                     AIRLV"
BASE COUNT           93 a           86 c           90 g           97 t
ORIGIN      
        1 gggtgagctg ttgctacacc ccggtatttc caccacggag gccagcatga acaacaataa
       61 cacattgtac gtcgggttag atgttcacaa agaatcgatt actgtcgctt atgctatcaa
      121 ttcagaacct gttgaactga tgggtaaaat tggcacatca cctactgata ttcagaatct
      181 ttgtaaacgt ctcaggtcaa agtcatcgca ggtcagtatc gtttatgaag cggggccctg
      241 tggttatgga ctttatcgtc ggctggtgaa atccggtttt gactgcatgg tctgcgcccc
      301 ctcacttatc ccgaaaaaac cgggggagcg ggttaaaacc gatcgccgtg atgccatcag
      361 acttgt
//
LOCUS       DAZPEP010000478          360 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZPEP010000478 DAZPEP010000000
VERSION     DAZPEP010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.481"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(16..240)
                     /locus_tag="ACHUT9_004725"
     CDS             complement(16..240)
                     /locus_tag="ACHUT9_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFR1350968.1"
                     /translation="MQQEPNGWKTVTTQFYIQEFSEYIAPLQDAVDLEIATEEERSLL
                     EAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
BASE COUNT          103 a           90 c           62 g          105 t
ORIGIN      
        1 gcataatccc aatgattact ccctgacagg atttgcaggc cactcaatat caggtgcagt
       61 tgatgtatca acacgattca acaatacccg atatttattc catgcctcca gcaacgatct
      121 ttcttcctcc gttgcgattt ccagatctac agcatcctgc agtggcgcaa tatactcact
      181 aaattcctgg atgtagaact gtgtggtgac ggtcttccag ccattcggct cctgctgtat
      241 cgaagcatac taggctattt caatatcgct atgctgcggc agcatttaac cccttgtaat
      301 tcatcgccat aattgattta attcacaaat aaaactataa catggtgaaa tcaatgaaaa
//
LOCUS       DAZPEP010000479          352 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZPEP010000479 DAZPEP010000000
VERSION     DAZPEP010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.482"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
BASE COUNT          126 a           53 c           69 g          104 t
ORIGIN      
        1 tgaccctaac aatagatcgg aattttctta gagtcaatgg tcactgtttt tgcttgcaaa
       61 gcaaatgttt tggtggttat aagttgtctt aatatagtca cgcatgcaac ttgttccatg
      121 cggagattaa agattggaat ataacgcgaa gatggaatta aattacattt gcaataatca
      181 atttattatt actcaataat taccgatatt attattgtaa attaatcatt agagtgcaaa
      241 aagaaatgta acggcaaaat aagaggacga tagctgagaa aaaagcgtgc tttcccaatc
      301 tggggcaaat cacgtctgaa tgacacctga aaacaggtgg tagcctcaag aa
//
LOCUS       DAZPEP010000480          342 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZPEP010000480 DAZPEP010000000
VERSION     DAZPEP010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.483"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>342)
                     /locus_tag="ACHUT9_004726"
     CDS             complement(<1..>342)
                     /locus_tag="ACHUT9_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFR1350969.1"
                     /translation="GLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGVEVD
                     KVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAATKG
                     FDDQTRRLKEN"
BASE COUNT           58 a          119 c           91 g           74 t
ORIGIN      
        1 tgttctcttt caggcggcgg gtctggtcat caaacccttt cgttgcggcc tcgttcgccg
       61 cctgcaatgc cccggcttca tcgccggaac gctgcaactg agcaacatac gcaatctgct
      121 ccgccgtcac gttatggaac tggcgtgcca tcgccgtcag ccccgacgtc gggtctgtgg
      181 tcagcttccc gaaggcttca gcgaccttgt ccacctccac gccggatgca gaggagaaac
      241 gcgccacact ctggctgatg gacgcaatct gagcctcacc gcttaccccc gccttaacca
      301 gtgcgctgag tgactcgctg gtctggttaa acgtcagccc tg
//
LOCUS       DAZPEP010000481          338 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZPEP010000481 DAZPEP010000000
VERSION     DAZPEP010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.484"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>338
                     /locus_tag="ACHUT9_004727"
     CDS             <1..>338
                     /locus_tag="ACHUT9_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350970.1"
                     /translation="LQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSYSTTGRLT
                     GVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDHHGR
                     LTEKTDLIPE"
BASE COUNT           82 a          124 c           86 g           46 t
ORIGIN      
        1 gttacagagc cagcacctga acagcctgct gtctgaccgc gattacacct ggaacgacaa
       61 cggcgaactc atccgcatca gcagcccgcg ccagacccgg agttacagct acagcaccac
      121 cggcaggctg accggcgttc acaccaccgc agcgaatctg gatatccgca tcccgtatgc
      181 cacagacccg gcggggaacc gcctgcccga cccggagctg cacccggaca gcaccctcag
      241 catgtggccg gataaccgta tcgcccgtga cgcgcactat ctttaccggt atgaccatca
      301 cggcagactg acagagaaaa ccgacctcat cccggaag
//
LOCUS       DAZPEP010000482          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZPEP010000482 DAZPEP010000000
VERSION     DAZPEP010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.485"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..332
                     /locus_tag="ACHUT9_004728"
                     /pseudo
     CDS             1..332
                     /locus_tag="ACHUT9_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           85 a           83 c           99 g           65 t
ORIGIN      
        1 gctatgcagc gaactgcata ttgccccgtc aacgtattac cactgtcagc aacagcgaca
       61 tcatccggat aaacgcagtg cccgtgcgca gcgcgatgac tggctgaaga aagagataca
      121 gcgcgtatac gatgaaaatc acaaggtata cggtgtaaag tctggcgtca gttgttacgg
      181 gaaggtatca gagtggccag atgcactgtg gcacgtctca tggcggttat gggacttgcc
      241 ggtgttctcc ggggtaaaaa ggtccgtacg accatcagcc ggaaagccgt tgccgcaggc
      301 gaccgcgtaa accgtcagtt cgtggcagaa cg
//
LOCUS       DAZPEP010000483          332 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZPEP010000483 DAZPEP010000000
VERSION     DAZPEP010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.486"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHUT9_004729"
                     /pseudo
     CDS             complement(30..279)
                     /gene="ymgC"
                     /locus_tag="ACHUT9_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
BASE COUNT          112 a           63 c           57 g          100 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaagttc taagagagca cggattccct gtcattaata
       61 atgatatccg ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga
      121 tcagaaagaa tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata
      181 tgactatcaa catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca
      241 cattgaaaaa ataaatctct ctgggattga attattcatg tcagcctcgt agaaatatta
      301 acctaataaa attatgctgt gtatagtcta aa
//
LOCUS       DAZPEP010000484          326 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZPEP010000484 DAZPEP010000000
VERSION     DAZPEP010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.487"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            79..258
                     /locus_tag="ACHUT9_004730"
                     /pseudo
     CDS             79..258
                     /locus_tag="ACHUT9_004730"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            206..>326
                     /locus_tag="ACHUT9_004731"
     CDS             206..>326
                     /locus_tag="ACHUT9_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFR1350971.1"
                     /translation="MVVGCSSKENALCHPQPKPPAPPAWAMMPPSNSLQLLDET"
BASE COUNT           92 a           86 c           84 g           64 t
ORIGIN      
        1 tcatgggctg ttaatcatta ccgtgataac gccattacct acaaagagca gcgcgacaaa
       61 aacgcaagag aactgaagct ggcgaacgca accattactg acatgcagca gcgccagcgt
      121 gatgctgatg cactcgatgc taaatacacg aaggagttag ctgatgcgaa agctgaaaat
      181 gatgctcttc ggcgcaagct tgataatggt ggtcgggtgc tcgtcaaagg aaaatgccct
      241 gtgccatcct cagccgaaac ctccagcgcc tccggcatgg gcaatgatgc caccgtcgaa
      301 ctctctccag ttgctggacg aaacgt
//
LOCUS       DAZPEP010000485          324 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZPEP010000485 DAZPEP010000000
VERSION     DAZPEP010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.488"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(1..48)
                     /locus_tag="ACHUT9_004732"
                     /pseudo
     CDS             complement(1..48)
                     /locus_tag="ACHUT9_004732"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27..>324)
                     /locus_tag="ACHUT9_004733"
     CDS             complement(27..>324)
                     /locus_tag="ACHUT9_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFR1350972.1"
                     /translation="QNLYLDNLEATGLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCG
                     DGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWHASAFTGICNDLAAASTFV"
BASE COUNT           70 a           98 c           90 g           66 t
ORIGIN      
        1 cgcccggatg gcttcggccc ccgttttcac acgaaggtcg atgcggcggc caaatcgttg
       61 caaatccccg taaaggcaga tgcgtgccat gcccggtgac gccagaggga gtgtgtgcgt
      121 cgctgccatt tgtcggtata cctctctcgt ttgctcagtt gttcaggaat atggtgcagc
      181 agctcgccgt caccacagta aatggcggca tgattcggca ccgatgaacc aaaacagcac
      241 agcagcacat cgcccggctg cgccgctgac aacggcacct gatacagccc tgtggcctcc
      301 agattatcca gatagagatt ctga
//
LOCUS       DAZPEP010000486          318 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZPEP010000486 DAZPEP010000000
VERSION     DAZPEP010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.489"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>318
                     /locus_tag="ACHUT9_004734"
     CDS             <1..>318
                     /locus_tag="ACHUT9_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFR1350973.1"
                     /translation="WARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYAS"
BASE COUNT           90 a           77 c           90 g           61 t
ORIGIN      
        1 tgggcccgtc gaccgtccgg cacggtccat cacagtgata aaggttctca gtatgtatcg
       61 ctggcctaca cacagcggct taaggaagcc ggattactgg catcaacagg aagtacaggc
      121 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
      181 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccacactcac gtgggtggac
      241 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      301 aaagcttatt atgcttcc
//
LOCUS       DAZPEP010000487          316 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZPEP010000487 DAZPEP010000000
VERSION     DAZPEP010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.490"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            81..>316
                     /locus_tag="ACHUT9_004735"
     CDS             81..>316
                     /locus_tag="ACHUT9_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFR1350974.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVY"
BASE COUNT           82 a           65 c           85 g           84 t
ORIGIN      
        1 gataaatcct catataacgc cattgaatgg tccgtctctc cggaaggttc ttcctgattt
       61 aatttatcgc gagaaaaact atgtctgtag tgatatcagg tgcgctgact gatggcgcag
      121 gcatccccat gtccggatgc cagattattc tgaaatcccg tgtaaacacc tcagaagtgg
      181 tgatgcgtac cagggctgat gtagtgaccg gaaataacgg tgaatattcg tttgaggcac
      241 aggtcggaaa atattgcgtg tatctgaaac gggactggcg cgacgagtac tgtgttggcg
      301 acattgctgt atacga
//
LOCUS       DAZPEP010000488          314 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZPEP010000488 DAZPEP010000000
VERSION     DAZPEP010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.491"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>314)
                     /locus_tag="ACHUT9_004736"
     CDS             complement(<1..>314)
                     /locus_tag="ACHUT9_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350975.1"
                     /translation="DYGTKSTNNTGAHTHSLSGSTSSAGAHQHSQTGPRTNSGSQPTG
                     MFPAGSTQVSGTNQVGISGSLTSGTSQWVGKSSSEGNHTHSLSGTAASAGAHAHTVGI
                     GA"
BASE COUNT           62 a           83 c           84 g           85 t
ORIGIN      
        1 tgagcaccaa taccgacagt atgcgcgtgt gcacccgcag atgctgctgt gccggacagt
       61 gagtgtgtat ggtttccttc tgaagatgat ttacctaccc attgggaggt gccactggtt
      121 aatgagccac tgataccaac ctgattagtc ccgctaactt gagtactccc tgccggaaac
      181 attccagtag gttgactccc gctattcgtt ctgggcccag tctgactatg ttggtgggcc
      241 ccagctgaac ttgtagagcc gctcagactg tgcgtatgcg ccccggtgtt attcgtggat
      301 ttagtgccgt aatc
//
LOCUS       DAZPEP010000489          308 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZPEP010000489 DAZPEP010000000
VERSION     DAZPEP010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.492"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>308
                     /locus_tag="ACHUT9_004737"
     CDS             <1..>308
                     /locus_tag="ACHUT9_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFR1350976.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     P"
BASE COUNT           67 a           82 c           92 g           67 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggaccc
//
LOCUS       DAZPEP010000490          307 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZPEP010000490 DAZPEP010000000
VERSION     DAZPEP010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.493"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>307)
                     /locus_tag="ACHUT9_004738"
     CDS             complement(<1..>307)
                     /locus_tag="ACHUT9_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFR1350977.1"
                     /translation="RQKLSDGWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVA
                     GAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVA"
BASE COUNT           77 a          100 c           80 g           50 t
ORIGIN      
        1 tgagccacac cgccgagcac catactggca ccgagagaaa acagaatgcc ggtcatacca
       61 ccggcaccaa tggcagcccc ccatgctgca agggtggctc cggcggtaaa gaatgatccg
      121 gcaatggcgg ctgctcccag gacaatctgg aatacaccac ctgacttggc cccggcgact
      181 ctgggaacaa tatgaatcac agcgccgtca ggcagagcct catgtaactg cgccgttaat
      241 ccggacgtgc tgacatcctg tccggcaata cgcacctgat accagccgtc gctcagtttc
      301 tgacgaa
//
LOCUS       DAZPEP010000491          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZPEP010000491 DAZPEP010000000
VERSION     DAZPEP010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.494"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUT9_004739"
     CDS             <1..>301
                     /locus_tag="ACHUT9_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFR1350978.1"
                     /translation="PVWAKCTKGVYPSYWEEWKKVREVVKVACPECGGKGEVSTACKD
                     CRGRGVAIHREESVKRGMPVIRDCQRCGGRGYERLPSTEAFNAICEVTNQITRAS"
BASE COUNT           76 a           56 c          100 g           69 t
ORIGIN      
        1 cgccggtatg ggcaaagtgt acgaaaggtg tgtatccgtc ttactgggaa gaatggaaaa
       61 aagtcaggga ggtggtaaaa gttgcctgtc cggagtgtgg cggaaagggt gaggtttcca
      121 ccgcctgtaa ggattgccgt gggcgtggtg tcgccattca tcgtgaagag tcggtaaaac
      181 gtggtatgcc tgttatcaga gactgccagc gttgtggtgg tcgtggctat gaaagactac
      241 catcaacgga ggcatttaat gctatatgcg aggtgacaaa ccagataaca cgcgcgtcat
      301 g
//
LOCUS       DAZPEP010000492          301 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZPEP010000492 DAZPEP010000000
VERSION     DAZPEP010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.495"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>301
                     /locus_tag="ACHUT9_004740"
     CDS             <1..>301
                     /locus_tag="ACHUT9_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350979.1"
                     /translation="DISHLSEGHRVTVHYGYDEKGRLTGERQTVHHPQTEALLWQHET
                     RHAYNAQGLANRCIPDSLSAVEWLTYGSGWLSGMKLGDTPLVEYTRDRLHRETLRS"
BASE COUNT           67 a           91 c          101 g           42 t
ORIGIN      
        1 gacatcagcc atctcagcga agggcaccgg gtgacggtgc attacgggta tgatgagaaa
       61 ggccggctga ccggtgagcg tcagacggtg catcacccgc agacggaagc actgctctgg
      121 cagcatgaga ccagacatgc gtacaacgcg caggggctgg cgaaccgctg tataccggac
      181 agcctgtccg ccgtggaatg gctgacctac ggcagcggct ggctgtcagg catgaaactc
      241 ggcgacacac cgctggtgga gtacacccgc gaccgcctgc accgggaaac gctgcgcagc
      301 t
//
LOCUS       DAZPEP010000493          298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZPEP010000493 DAZPEP010000000
VERSION     DAZPEP010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.496"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..199
                     /locus_tag="ACHUT9_004741"
                     /pseudo
     CDS             1..199
                     /locus_tag="ACHUT9_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT           92 a           67 c           68 g           71 t
ORIGIN      
        1 ccttcacgat aaaaaacttt tcattaaagg taaagcggat caatatccgg cacttcattc
       61 tctgatatct ccgacccgca ttaatctgaa agaaatagaa attcactggc gtgaagtgct
      121 acgtctggcg acctcaataa aacagggaac agtgacagca tccctgatgc tgagaaaact
      181 tgccagttac cccaaacaaa agtaaggcag tatgactttc cgtgagacag atgctggcca
      241 acgtgggata tttaacgcgg gttaacatcg ttgtcagagc cgcatgggtg agctgttg
//
LOCUS       DAZPEP010000494          298 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZPEP010000494 DAZPEP010000000
VERSION     DAZPEP010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.497"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..207
                     /locus_tag="ACHUT9_004742"
     CDS             <1..207
                     /locus_tag="ACHUT9_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350980.1"
                     /translation="DAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIELEGV
                     EYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          105 a           57 c           70 g           66 t
ORIGIN      
        1 gacgccaaac gcgcaaaagc tgatttgctg gaaaaaacat tcacctcccg ctcatgggaa
       61 agaatcaaag gtatggactg gccgacaatc aaagctggtc gtaatgcact atggattgaa
      121 ctggaaggcg ttgaatatgc attcccggat ccacaaacac aaaacgaaac gcaacaagca
      181 ggatacgatg agaatattcc ggtgtaaaac caagtgcatt ttggacacgg tttttgaaaa
      241 tattaatgga ggtgttcatg tataaaatta cagccactat tgaaaaggaa ggtggttc
//
LOCUS       DAZPEP010000495          295 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZPEP010000495 DAZPEP010000000
VERSION     DAZPEP010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.498"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..279
                     /locus_tag="ACHUT9_004743"
     CDS             <1..279
                     /locus_tag="ACHUT9_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HFR1350981.1"
                     /translation="LTYYADDGWAEADNNIAENALRMVSLGRKNYLFFGSDHGGERGA
                     LLYSLIGTCKLNGVEPESYLRYVLDVIADWPINRVGELLPWRVALPTE"
BASE COUNT           70 a           77 c           89 g           59 t
ORIGIN      
        1 ctgacgtact atgcagatga tggctgggct gaggcggaca ataacatcgc tgaaaatgcg
       61 ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct tcggttcgga tcatggagga
      121 gagcggggag cgctgctgta cagcctgatc gggacgtgca aactgaacgg agtggagcca
      181 gaaagctacc tccgctatgt ccttgacgtc atagccgact ggccgataaa ccgggtcggc
      241 gaactgctcc cctggcgcgt agcactgccg actgaataac acattcccgt caata
//
LOCUS       DAZPEP010000496          292 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZPEP010000496 DAZPEP010000000
VERSION     DAZPEP010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.499"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(56..>292)
                     /locus_tag="ACHUT9_004744"
     CDS             complement(56..>292)
                     /locus_tag="ACHUT9_004744"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350982.1"
                     /translation="TKVFAKIFDIKSSNILCRIFSFLYFILYMAITFLVTNFIFTTAM
                     SLHGGDGGLRACFAIGCFVVAVEQTRKFYSLLIK"
BASE COUNT          106 a           60 c           40 g           86 t
ORIGIN      
        1 atttgatgaa agtacttacc cttcatcaaa tacacatttc tttagtactt gccctttact
       61 tgataagcaa actatagaat tttcttgtct gctcaactgc aacgacaaaa cagccaattg
      121 caaaacatgc tctcaaccca ccatccccgc cgtgtaatga cattgcggta gtgaatataa
      181 aattagtcac caagaaagta atagccatat acaagatgaa atatagaaat gagaaaatgc
      241 ggcaaagtat attactgctt ttaatatcaa atatctttgc aaatactttc gt
//
LOCUS       DAZPEP010000497          290 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZPEP010000497 DAZPEP010000000
VERSION     DAZPEP010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.500"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..241
                     /locus_tag="ACHUT9_004745"
                     /pseudo
     CDS             1..241
                     /locus_tag="ACHUT9_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365007.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          116 a           52 c           39 g           83 t
ORIGIN      
        1 tttcgataac agatgattta atgagtatgg ctgtaaatga actcataaaa ctgaccaaaa
       61 tagagaataa tgtagacctg aataaattca ctactatatg cacagacgtt ctatcccccc
      121 tcgtcaccag acataataaa gaaaaaaaac aaacgacatt ctacccttct caaaaatccc
      181 ctttttaatt ttcattgaaa aaatggcaat tgatactatg aatccaggtc atacacatta
      241 aggcatttat gtaatgtcgt aattaagata actaataagg tgaatattag
//
LOCUS       DAZPEP010000498          286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZPEP010000498 DAZPEP010000000
VERSION     DAZPEP010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.501"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
BASE COUNT           97 a           67 c           58 g           64 t
ORIGIN      
        1 ctgtacaaaa aaccgccagc caaggcagtt aagagtgtgt tcccggggtt tgcttagaat
       61 attttgtaag ttgtccgaag agatatttac aacaccagaa tgatgaatca tcagtcccct
      121 gccagaggtt cactacgtaa cctgaccgac aatgcaccat tctggtgctg aaaaaagagc
      181 actggaactg caacaaaaca acatcacaga acaggagatg gtaaggagtc agacattccc
      241 acacaatatt gtgtcagtgc aaataacaac cttcgtctca aatctc
//
LOCUS       DAZPEP010000499          286 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZPEP010000499 DAZPEP010000000
VERSION     DAZPEP010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..286
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.502"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
BASE COUNT          118 a           49 c           37 g           82 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagccaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacga
//
LOCUS       DAZPEP010000500          284 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZPEP010000500 DAZPEP010000000
VERSION     DAZPEP010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..284
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.503"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>284
                     /locus_tag="ACHUT9_004746"
     CDS             <1..>284
                     /locus_tag="ACHUT9_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HFR1350983.1"
                     /translation="RLISGKTQNDQDICALAKEYEIAMLLVTKGAEGVVVCYRGQVHH
                     FAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRIIDLAQRCGA"
BASE COUNT           72 a           55 c           88 g           69 t
ORIGIN      
        1 gcgacttatc agtggaaaaa cacagaacga tcaggatata tgcgccctgg caaaagagta
       61 tgagatcgcc atgctgttgg tgactaaagg tgcagaaggg gtggtggtct gttatcgagg
      121 acaagttcac cattttgctg gaatgtctgt gaattgtgtc gatagcacgg gggcgggaga
      181 tgcgttcgtt gccgggttac tcacaggtct gtcctctacg ggattatcta cagatgagag
      241 agaaatgcga cgaattatcg atctcgctca acgttgcgga gcgc
//
LOCUS       DAZPEP010000501          270 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZPEP010000501 DAZPEP010000000
VERSION     DAZPEP010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.504"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>270)
                     /locus_tag="ACHUT9_004747"
     rRNA            complement(<1..>270)
                     /locus_tag="ACHUT9_004747"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           49 a           82 c           62 g           77 t
ORIGIN      
        1 gcgtgccttc tcccgaagtt acggcaccat tttgcctagt tccttcaccc gagttctctc
       61 aagcgccttg gtattctcta cctgaccacc tgtgtcggtt tggggtacga tttgatgtta
      121 cctgatgctt agaggctttt cctggaagca gggcatttgt tgcttcagca ccgtagtgcc
      181 tcgtcatcac gcctcagcct tgattttccg gatttgcctg gaaaaccagc ctacacgctt
      241 aaaccgggac aaccgtcgcc cggccaacat
//
LOCUS       DAZPEP010000502          263 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZPEP010000502 DAZPEP010000000
VERSION     DAZPEP010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.505"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            41..>263
                     /locus_tag="ACHUT9_004748"
     CDS             41..>263
                     /locus_tag="ACHUT9_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFR1350984.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPA"
BASE COUNT           63 a           58 c           89 g           53 t
ORIGIN      
        1 ctgatgacag tatcagaagg gatcgcagga ggagtccggt atggctgaac cggtaggcga
       61 tctggtcgtt gatttgagtc tggatgcggc cagatttgac gagcagatgg ccagagtcag
      121 gcgtcatttt tccggtacgg aaagtgatgc gaaaaaaaca gcggcagtcg ttgaacagtc
      181 gctgagccga caggcgctgg ctgcacagaa agcggggatt tccgtcgggc agtataaagc
      241 cgccatgcgt atgctgcctg cac
//
LOCUS       DAZPEP010000503          258 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZPEP010000503 DAZPEP010000000
VERSION     DAZPEP010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.506"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..161
                     /locus_tag="ACHUT9_004749"
     CDS             <1..161
                     /locus_tag="ACHUT9_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFR1350985.1"
                     /translation="VKLSMPELEELAAAMVQAVVERNDEIYRRQREMKEELSSLDDLA
                     SIRAFDVK"
BASE COUNT           64 a           53 c           86 g           55 t
ORIGIN      
        1 aggtgaaact gtcgatgccg gaactggaag aactggcggc agcaatggtg caggctgttg
       61 ttgaacgcaa tgacgagatt tatcgccgtc agcgtgaaat gaaagaggag ctgagcagtc
      121 tggatgattt ggcttcaatt cgggcgtttg acgttaagta atgaataagc cgcaactggc
      181 ggaatcacag aagaccgctt tgcttaccga ggcggagtct gtcatccggc cgccggggtg
      241 tgcggtcagg ctgaacag
//
LOCUS       DAZPEP010000504          251 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZPEP010000504 DAZPEP010000000
VERSION     DAZPEP010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.507"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>251
                     /locus_tag="ACHUT9_004750"
     rRNA            <1..>251
                     /locus_tag="ACHUT9_004750"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           67 a           57 c           81 g           46 t
ORIGIN      
        1 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
       61 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
      121 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
      181 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
      241 ctgtaagcct g
//
LOCUS       DAZPEP010000505          250 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZPEP010000505 DAZPEP010000000
VERSION     DAZPEP010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.508"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>250)
                     /locus_tag="ACHUT9_004751"
     CDS             complement(<1..>250)
                     /locus_tag="ACHUT9_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350986.1"
                     /translation="TLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVIRMHDER
                     THHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT           35 a           78 c           80 g           57 t
ORIGIN      
        1 cccgtccggc ggcccagcgg gtcatagagg taccggctct ccacctgtgg ttcgccgtgc
       61 tgtatccgcg tgtagaacac caggcggtgc tggctgtcgt agtgatagtg gtgggtgcgc
      121 tcgtcgtgca tccggataac cccttccggg atacggtccg tcttctccgc cagcctgccg
      181 tattcatcgt agcgatagac atagtgcgca tcttccgcga tgcggttatc cggccacgcc
      241 gtgagggtgc
//
LOCUS       DAZPEP010000506          247 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZPEP010000506 DAZPEP010000000
VERSION     DAZPEP010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.509"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>247
                     /locus_tag="ACHUT9_004752"
     CDS             <1..>247
                     /locus_tag="ACHUT9_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001388905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1350987.1"
                     /translation="LMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGSRL
                     EDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTET"
BASE COUNT           86 a           49 c           56 g           56 t
ORIGIN      
        1 tttaatgaat gaaggattct taactaaaaa ggatttgaaa gactttgcca ataactctgc
       61 tggtttaatt tcaaaagctg tcaacggaat ggtgaaaaag ggctataccg acgcacagat
      121 cggtagtcgt ctggaagatt tagcggacga cctcctctta acttctaaat attggcaaag
      181 atcagccaga gaggtgcagg caagcattac ccagcaaaaa gccgccggac aatggctaac
      241 agaaaca
//
LOCUS       DAZPEP010000507          244 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZPEP010000507 DAZPEP010000000
VERSION     DAZPEP010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.510"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>244
                     /locus_tag="ACHUT9_004753"
     CDS             <1..>244
                     /locus_tag="ACHUT9_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFR1350988.1"
                     /translation="VLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITYPARFQL
                     VAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLD"
BASE COUNT           59 a           74 c           54 g           57 t
ORIGIN      
        1 gtgctttttc ttgatgagct acctgaattt gaacggcgta cactggatgc cttgcgagag
       61 ccgattgaat ccgggcagat ccatctttca cgcacacgag caaaaataac ctatccagcc
      121 cgtttccagc ttgttgcggc gatgaatccc agccctaccg gacattatca gggaaaccat
      181 aaccgctgca cgccagaaca gacattacgt tatctcaacc ggctctcggg gccctttctc
      241 gacc
//
LOCUS       DAZPEP010000508          242 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZPEP010000508 DAZPEP010000000
VERSION     DAZPEP010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.511"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..220
                     /locus_tag="ACHUT9_004754"
     CDS             <1..220
                     /locus_tag="ACHUT9_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFR1350989.1"
                     /translation="RKGNCYDNAPMESFWGTLKNESLSHYRFNNRDEAISVIREYIEI
                     FYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT           75 a           52 c           58 g           57 t
ORIGIN      
        1 gcgtaaaggt aactgttacg acaacgctcc gatggaaagc ttctggggaa cgctgaaaaa
       61 tgagagcctg agccactatc gttttaataa ccgggatgaa gccatctcag taatacggga
      121 atacattgag attttctaca atcgtcagcg tcgtcactct cgtctgggga atatctcccc
      181 ggcagccttc agggaaaaat atcatcagat ggctgcttaa aaaaagaaca aatggtagtg
      241 tc
//
LOCUS       DAZPEP010000509          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZPEP010000509 DAZPEP010000000
VERSION     DAZPEP010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.512"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(<1..>241)
                     /locus_tag="ACHUT9_004755"
     CDS             complement(<1..>241)
                     /locus_tag="ACHUT9_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFR1350990.1"
                     /translation="ECQIRNSNFDNCYLYASHFTRAEFLSAKEISFIKSNLTAVMFDH
                     VRMSTGNFKDCITEQLELTIDYSDIFGNEDLDGYIN"
BASE COUNT           83 a           44 c           35 g           79 t
ORIGIN      
        1 attgatataa ccatcgagat cttcattccc aaatatatct gaataatcaa tagttaattc
       61 caattgttct gtaatgcaat ctttaaaatt ccctgtcgac attcgcacat gatcaaacat
      121 aacagctgtc aaattcgatt taataaatga tatttctttg gcagacagaa actctgctct
      181 ggtgaagtgc gaagcgtaaa gataacaatt atcgaagttt gaatttctaa tctgacattc
      241 g
//
LOCUS       DAZPEP010000510          241 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZPEP010000510 DAZPEP010000000
VERSION     DAZPEP010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.513"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>241
                     /locus_tag="ACHUT9_004756"
     CDS             <1..>241
                     /locus_tag="ACHUT9_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFR1350991.1"
                     /translation="DLLEKPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEID
                     DLNAVNNYLLNNKKATDNEINDIKVNLSHILIDSLD"
BASE COUNT           94 a           33 c           37 g           77 t
ORIGIN      
        1 gatttattag aaaaaccatt gaacaatgca ttattagcaa taaggaaaga acatctgcta
       61 ttaatgcctt atgtatgtaa tgaatcgatt tcatacttgc ttggtgaaaa agggatactg
      121 aaagaaatag atgaccttaa cgcagtaaat aattatcttc ttaataataa gaaagctact
      181 gacaatgaaa ttaatgacat caaagttaac ctgtctcata ttcttattga ttccttagat
      241 g
//
LOCUS       DAZPEP010000511          240 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZPEP010000511 DAZPEP010000000
VERSION     DAZPEP010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.denovo.514"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            <1..>240
                     /locus_tag="ACHUT9_004757"
     rRNA            <1..>240
                     /locus_tag="ACHUT9_004757"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           61 a           55 c           80 g           44 t
ORIGIN      
        1 ggacgaagac tgacgctcag gtgcgaaagc gtggggagca aacaggatta gataccctgg
       61 tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt ggcttccgga
      121 gctaacgcgt taagtcgacc gcctggggag tacggccgca aggttaaaac tcaaatgaat
      181 tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac gcgaagaacc
//
LOCUS       DAZPEP010000512        10197 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.guided.144, whole genome shotgun sequence.
ACCESSION   DAZPEP010000512 DAZPEP010000000
VERSION     DAZPEP010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10197
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.guided.144"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            1..652
                     /locus_tag="ACHUT9_004758"
                     /pseudo
     CDS             1..652
                     /locus_tag="ACHUT9_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHUT9_004759"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHUT9_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFR1350992.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUT9_004760"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHUT9_004760"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFR1350993.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUT9_004761"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHUT9_004761"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFR1350994.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUT9_004762"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHUT9_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFR1350995.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHUT9_004763"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHUT9_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFR1350996.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHUT9_004764"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHUT9_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFR1350997.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6673..7776
                     /gene="proB"
                     /locus_tag="ACHUT9_004765"
     CDS             6673..7776
                     /gene="proB"
                     /locus_tag="ACHUT9_004765"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFR1350998.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7788..9041
                     /gene="proA"
                     /locus_tag="ACHUT9_004766"
     CDS             7788..9041
                     /gene="proA"
                     /locus_tag="ACHUT9_004766"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFR1350999.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9156..9231
                     /locus_tag="ACHUT9_004767"
     tRNA            9156..9231
                     /locus_tag="ACHUT9_004767"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9189..9191,aa:Thr,seq:cgt)
     gene            9837..9995
                     /locus_tag="ACHUT9_004768"
     CDS             9837..9995
                     /locus_tag="ACHUT9_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFR1351000.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2530 a         2466 c         2650 g         2551 t
ORIGIN      
        1 acaccccatt ataaggggta cgaataaagt aacggggcag tctattttac agcggcatat
       61 tgcctcgttt tcgcatcatg gtttgcctga aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgcg tattaatcgc cagctgatct ctttacgcat
      181 aatgcaacag gttaaggcca ccggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcact cggtgattac tcctagcttg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggct gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacagcgacg cagctctcac gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggct ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggag
      721 cgattaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcagct ctggtttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg cggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagtg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gcgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gccagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcaga
     1681 gaacggatca gacagtgaat ttctacgtta ttatcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tctgagttgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc tgcatgaccc gctaggaagc gcaccagcag tttgttggca
     2101 ttacccaggc caacgtggat ttccccgccg gagtgacccc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccga acgcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc gggccgtgaa ccacgttttc gtcagccaga
     2401 accgccagcg cagacgccat accaatgccg ttatctgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccga tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgcgagctt aaagtgctaa aacgcgcaga aggcgatggc gaaggcttca tcgttattga
     3301 tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt gacccacgtg
     3781 aaatcctcga cgtccaggcg cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt
     3841 tatacgatac ggtgattggc taccgtggtg gcaactggat ttatgagtgg gccatccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgtcact
     3961 ggctgcatgc ggctacgttg tacaacattg ctgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttgtcaaacc gtgcctatga agaagccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcatcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gttttaaagg ctctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctgggg cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcttgtgtt aaaaatttgc taaaatttgc caatttggta
     4861 aaacagttgc atcacaacag gagattgcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgagttctg gggctggtgg atggagcttg aagcgcagga atcccgtttt acctacagtt
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagacactg
     5161 aagtggttga acgactggag cacaccctac gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgatgaa ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgta tcacatccgg caatatccat taaaactgat
     5341 aggtcatgcc aaccgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaacgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattgacc agatcttcat caccgatacc ttcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgtgtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gcctgtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcccc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttctgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttcctgattt taattaacgc gcgaatattc
     6421 agcgggagag ccccgttgaa aacaggaaag tttttaacct gagattgtta aagatatatt
     6481 acagattaat aatattctta aaatgtggta atttattaaa tctgtaataa aagcgtaaac
     6541 aactgccgct aggcttgctg atcccgcgca acaaaacgcc atgctttgct cgcagatggt
     6601 tggcaaccga cgacagtcct gctaaaacgt tcgtttgata tcatttttcc taaaattgaa
     6661 tggcagagaa tcatgagtga cagccagacg ctggtggtaa aactcggcac cagtgtgcta
     6721 acaggcggat cgcgccgtct gaaccgtgcc catattgttg aacttgttcg ccagtgcgcg
     6781 cagttacatg ccgccgggca tcggattgtt attgtgacgt cgggcgcgat cgccgccgga
     6841 cgtgagcacc tgggttaccc ggaactgcca gcgactatcg cctcgaaaca actgctggca
     6901 gcggtagggc agagtcgact gattcaactg tgggaacagc tgttttcgat ttatggcatt
     6961 cacgtcgggc aaatgctgct gacccgtgct gatatggaag accgtgaacg cttcctgaac
     7021 gcccgcgaca ccctgcgtgc gttgctcgat aacaatatcg ttccggtaat caatgagaac
     7081 gatgctgtcg ctacggcaga gattaaagtc ggcgataacg ataacctttc tgcgctggcg
     7141 gcgattctgg cgggtgccga taaactgttg ctgctgaccg atcaaaaagg tttgtatacc
     7201 gctgacccgc gcagcaatcc gcaggcagaa ctgattaaag atgtttacgg cattgatgac
     7261 gcactgcgcg cgattgccgg tgacagcgtt tcaggcctcg gaactggcgg catgagtacc
     7321 aaattgcagg ccgctgacgt ggcttgccgt gcgggtatcg acaccattat tgccgcgggc
     7381 agcaagccgg gcgttattgg tgatgtgatg gaaggcattt ccgtcggtac gctgttccat
     7441 gcccaggcga ctccgcttga aaaccgtaaa cgctggattt tcggtgcgcc gccggcgggt
     7501 gaaatcacgg tagatgaagg ggcaactgcc gccattctgg aacgcggcag ctccctgttg
     7561 ccgaaaggca ttaaaagcgt gactggcaat ttctcgcgtg gtgaagtcat ccgcatttgc
     7621 aacctcgaag gccgcgatat cgcccacggc gtcagtcgtt acaacagcga tgcattacgc
     7681 cgtattgccg gacaccactc gcaagaaatt gatgcaatac tgggatatga atacggcccg
     7741 gttgccgttc accgtgatga catgattacc cgttaaggag ctggccgatg ctggaacaaa
     7801 tgggcattgc tgcgaagcaa gcctcgtata aattagcgca actctccagc cgcgaaaaaa
     7861 atcgcgtgct ggaaaaaatc gccgatgaac tggaagcaca aagcgaaatc atcctcaacg
     7921 ctaacgccca ggatgttgct gacgcgcgag ccaatggcct tagcgaagcg atgcttgacc
     7981 gtctggcact gacgcccgca cggctgaaag gcattgccga cgatgtacgt caggtgtgca
     8041 acctcgccga tccggtgggg caggtaatcg atggcggcgt actggacagc ggcctgcgtc
     8101 ttgagcgtcg tcgcgtaccg ctgggggtta ttggcgtgat ttatgaagcg cgcccgaacg
     8161 tgacggttga tgtcgcttcg ctgtgcctga aaaccggtaa tgcggtgatc ctgcgcggtg
     8221 gcaaagaaac gtgtcgcact aacgctgcaa cggtggcggt gattcaggac gccctgaaat
     8281 cctgcggctt accggcgggt gccgtgcagg cgattgataa tcctgaccgt gcgctggtca
     8341 gtgaaatgct gcgtatggat aaatacatcg acatgctgat cccgcgtggt ggcgctggtt
     8401 tgcataaact gtgccgtgaa cagtcgacaa tcccggtgat cacaggtggt ataggcgtat
     8461 gccatattta cgttgatgaa agtgtagaga tcgctgaagc attaaaagtg atcgtcaacg
     8521 cgaaaactca gcgtccgagc acatgtaata cggttgaaac gttgctggtg aataaaaaca
     8581 tcgccgatag cttcctgccc gcattaagca aacaaatggc ggaaagcggc gtgacattac
     8641 acgcagatgc agctgcactg gcgcagttgc aggcaggccc tgcgaaggtg gttgctgtta
     8701 aagccgaaga gtatgacgat gagtttctgt cattagattt gaacgtcaaa atcgtcagcg
     8761 atcttgacga tgccatcgcc catattcgtg aacacggcac acaacactcc gatgcgatcc
     8821 tgacccgcga tatgcgcaac gcccagcgtt ttgttaacga agtggattcg tccgctgttt
     8881 acgttaacgc ctctacgcgt tttaccgacg gcggccagtt tggtctgggt gcggaagtgg
     8941 cggtaagcac acaaaaactc cacgcgcgtg gcccaatggg gctggaagca ctgaccactt
     9001 acaagtggat cggcattggt gattacacca ttcgtgcgta aataaaaccg ggtgatgcaa
     9061 aagtagccat ttgattcaca aggccattga cgcatcgccc ggttagtttt aaccttgtcc
     9121 accgtgattc acgttcgtga acatgtcctt tcagggccga tatagctcag ttggtagagc
     9181 agcgcattcg taatgcgaag gtcgtaggtt cgactcctat tatcggcacc attaagaaaa
     9241 ttaataactt agcttaactt ctctaagaaa ttgtatcttc tgttatcact tacaaacgcc
     9301 tatacgttgt tatgggcttt ggtagtgtaa cacgtatggg cgagtagttc ccaggctcaa
     9361 cctcgtaaca agttcctttg aagctggaag acgtgccacc gtctctaaac cttctaccaa
     9421 aactcttagc aagatgttcc acacctggcg cggtgtccaa tgatgctaca cgtcgtgaga
     9481 cgttgcagag gataaacagg ctaaatgatg ggaagagttt taagtgcaat agtggattaa
     9541 agcccacctc ttcaaaacct aacttgatcg ggaaagaatc aagctctagc acggtttcgt
     9601 ccaccggagt acaacgggtg cctgataaga gaagaactgc taagacttca tagcttttgt
     9661 gaaaaagccc tttttagtgg atacgcctac ggcgcttatt ttttggtata accatcaaag
     9721 gcgctttatg gctcctattg tcttgaaaat gtatctttac ttgtattgtg ttttatgcgt
     9781 agtctccctg tgtagtatgt aacttgttaa ttttcatatg aatacagagg cacacgatga
     9841 aaaacattgc agcaatcaaa cgtaataacc gcaagattca cgctcgtaag ttcctgtcta
     9901 cgccagaagg aaaagcctgg ctagagcgta aacagagaga gaacgaagaa agaaaactcc
     9961 ttagtgagtt gaaatggctt agagaggatt tctgaaaatc tccagaacgc aacaggacgc
    10021 attctaacgc tgttttcatt actcggtata gggataggtg atgtgtgttt aatgcgtctg
    10081 tgcgcttgtt ttgtgcctta ttttgcattt tcataatgcg gaagagaaca cggaaaacct
    10141 taaaattaat tattggggta tttaaggcta ttttcaggtg atttaagggt attaaac
//
LOCUS       DAZPEP010000513         6302 bp    DNA     linear   BCT 24-OCT-2024
DEFINITION  TPA_asm: Shigella sonnei strain M1801013
            SAMN43277190-rid23488123.guided.205, whole genome shotgun sequence.
ACCESSION   DAZPEP010000513 DAZPEP010000000
VERSION     DAZPEP010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43277190
            Sequence Read Archive: SRR30421716
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 27-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002396376.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002396376.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/24/2024 09:03:32
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 4,775
            CDSs (total)                      :: 4,689
            Genes (coding)                    :: 4,419
            CDSs (with protein)               :: 4,419
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 67
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 270
            CDSs (without protein)            :: 270
            Pseudo Genes (ambiguous residues) :: 0 of 270
            Pseudo Genes (frameshifted)       :: 123 of 270
            Pseudo Genes (incomplete)         :: 90 of 270
            Pseudo Genes (internal stop)      :: 88 of 270
            Pseudo Genes (multiple problems)  :: 30 of 270
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6302
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43277190-rid23488123.guided.205"
                     /strain="M1801013"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /geo_loc_name="Australia: Queensland"
                     /collection_date="2018-02-01"
                     /collected_by="FSS"
     gene            complement(34..204)
                     /locus_tag="ACHUT9_004769"
                     /pseudo
     CDS             complement(34..204)
                     /locus_tag="ACHUT9_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUT9_004770"
     CDS             complement(423..1400)
                     /gene="epmA"
                     /locus_tag="ACHUT9_004770"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFR1351001.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUT9_004771"
     CDS             1725..3533
                     /gene="frdA"
                     /locus_tag="ACHUT9_004771"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFR1351002.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUT9_004772"
     CDS             3526..4260
                     /gene="frdB"
                     /locus_tag="ACHUT9_004772"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFR1351003.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUT9_004773"
     CDS             4271..4666
                     /gene="frdC"
                     /locus_tag="ACHUT9_004773"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFR1351004.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUT9_004774"
     CDS             4677..5036
                     /gene="frdD"
                     /locus_tag="ACHUT9_004774"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFR1351005.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5099..6232
                     /locus_tag="ACHUT9_004775"
     CDS             5099..6232
                     /locus_tag="ACHUT9_004775"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFR1351006.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT         1600 a         1629 c         1626 g         1447 t
ORIGIN      
        1 ccatcataca ctaaatcagt aagttggcag catcaccatt aaggcgaatg gaataaagaa
       61 taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt aataagccga
      121 tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc ctatcagact
      181 catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg tataacacgt
      241 gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat acagaattga
      301 tacccggatg atatcaaata tccagtctta aattgtggcc tttaacggaa aaatgaaaac
      361 atatgctaca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt aatttttcag
      421 aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc
      481 aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat accgactttc
      541 aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta
      601 cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc atggaaacca
      661 ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg atgatcttcg
      721 gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt
      781 tctttgccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa cgtgtcgcgg
      841 tcttcttcgg tatcagcaac gttactcaaa tccagtttcg ctgcgacttc ccgcagttgc
      901 gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg ttgataagaa
      961 aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc
     1021 aaccggtaca tatcatagtg cggtcgatac cactcaagca tagtgaactc agggttgtga
     1081 taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg cccacaaccg
     1141 gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag attcatcccc
     1201 tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc
     1261 gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc aaagaaacga
     1321 cggatctccg ccataatcgc cgcacgtttt aataagttag gaatggatgc gctcggctgc
     1381 caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac gaagtctact
     1441 cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg cagtgactaa
     1501 aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat aatttagccc
     1561 tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca gactatactg
     1621 ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca ggcacttacc
     1681 ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag
     1741 ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt gctgccgcgc
     1801 aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg cgtagccata
     1861 ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc
     1921 actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc gtggattatt
     1981 tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtgggggtgc ccatggagcc
     2041 gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct
     2101 ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag acctctctgc
     2161 aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg gttgatgatg
     2221 gtcatgttcg cggcctggta gcaatgaaca tgatggaagg cacgctggtg cagatccgtg
     2281 ctaacgcggt cgttatggct actggcggtg cgggtcgcgt ttatcgttac aacaccaacg
     2341 gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg
     2401 acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc ctgatgaccg
     2461 aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag
     2521 attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat atggaactgg
     2581 gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc aacaccatct
     2641 ccacgccgcg tggcgatgtg gtttatctcg acttgcgtca cctcggcgag aaaaaactgc
     2701 atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta
     2761 aagaaccgat tccggtacgt ccgaccgcac actacaccat gggcggtatc gaaaccgatc
     2821 agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc
     2881 tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc ttcggccgtc
     2941 tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac gaagcggcaa
     3001 ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctggttaac caggatggcg
     3061 gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta
     3121 tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac
     3181 gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac ctgctctaca
     3241 ccattgaact gggccacggt ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac
     3301 gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg
     3361 tcaacttcct caaacacacc ctcgccttcc gcgatgctga tggcacgact cgcctagagt
     3421 acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt ggcgaagcgg
     3481 atgcagccga taaggcggaa gcagccaata agaaggagaa ggcgaatggc tgagatgaaa
     3541 aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc gcatagcgca
     3601 ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg ctacatcaaa
     3661 gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc
     3721 tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac
     3781 accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga tctggtggtc
     3841 gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat cggcaactcc
     3901 cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa gtatcaccag
     3961 ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac
     4021 ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga agatagccgc
     4081 gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggcgt atggagctgt
     4141 actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt
     4201 cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa accacgctaa
     4261 ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg
     4321 gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt
     4381 gtggttcagc attgaactga ttttcgggct gtttgccctg aaaaatggcc cggaagcctg
     4441 ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc tgatcactct
     4501 ggcggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag cggccaatat
     4561 cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct gggcggtaac
     4621 tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag cctgagatga
     4681 ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc ggggccggtg
     4741 gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt ctgctgccac
     4801 tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc gcgcagagct
     4861 tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc
     4921 gtatgcacca cgcgatgcac gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct
     4981 acggtctggc tgctatcctg acagttgtca cgctgattgg tatcgttaca atctaacgta
     5041 tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac cagaccttat
     5101 gttcaaaacg acgctctgcg ccttattaat taccgcctct tgctccacat ttgctgcccc
     5161 tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc aacaaaagat
     5221 ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta cctggggcta
     5281 tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt taggttcggt
     5341 cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg aaatcaagtt
     5401 aagcgatccc gcaacaaaat actggcctga acttactgct aaacagtgga atgggatcac
     5461 actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc cggatgaagt
     5521 gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat gggctccagg
     5581 aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg ctgtgaagcc
     5641 gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac tcaaactcaa
     5701 ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg gatatcgcga
     5761 aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg gtgtgaagtc
     5821 gaccattgaa gatatggccc gctgggtgca aagcaattta accccccttg atatcaatga
     5881 gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa ccggcgatat
     5941 gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca gcatcattaa
     6001 cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta agcccccaac
     6061 tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat ttggtagcta
     6121 tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca aaaactatcc
     6181 caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt aaaatttcat
     6241 cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac acttagaaaa
     6301 aa
//
